LOCUS       DAJSVF010000001        90965 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSVF010000001 DAJSVF010000000
VERSION     DAJSVF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.001"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(43..1083)
                     /locus_tag="ON550_000001"
     CDS             complement(43..1083)
                     /locus_tag="ON550_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3953120.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1360..3162)
                     /locus_tag="ON550_000002"
     CDS             complement(1360..3162)
                     /locus_tag="ON550_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953121.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3176..3928)
                     /locus_tag="ON550_000003"
     CDS             complement(3176..3928)
                     /locus_tag="ON550_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953122.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3937..4299)
                     /locus_tag="ON550_000004"
     CDS             complement(3937..4299)
                     /locus_tag="ON550_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3953123.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4475..5347)
                     /locus_tag="ON550_000005"
     CDS             complement(4475..5347)
                     /locus_tag="ON550_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3953124.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5398..5886)
                     /locus_tag="ON550_000006"
     CDS             complement(5398..5886)
                     /locus_tag="ON550_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3953125.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(6024..6815)
                     /locus_tag="ON550_000007"
     CDS             complement(6024..6815)
                     /locus_tag="ON550_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR3953126.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6870..7478)
                     /locus_tag="ON550_000008"
     CDS             complement(6870..7478)
                     /locus_tag="ON550_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR3953127.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7471..7788)
                     /locus_tag="ON550_000009"
     CDS             complement(7471..7788)
                     /locus_tag="ON550_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3953128.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7944..8672)
                     /locus_tag="ON550_000010"
     CDS             complement(7944..8672)
                     /locus_tag="ON550_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR3953129.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8831..9712
                     /locus_tag="ON550_000011"
     CDS             8831..9712
                     /locus_tag="ON550_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3953130.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9949..10992)
                     /locus_tag="ON550_000012"
     CDS             complement(9949..10992)
                     /locus_tag="ON550_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3953131.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10995..11663)
                     /locus_tag="ON550_000013"
     CDS             complement(10995..11663)
                     /locus_tag="ON550_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3953132.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11805..14141
                     /locus_tag="ON550_000014"
     CDS             11805..14141
                     /locus_tag="ON550_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3953133.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14256..14567)
                     /locus_tag="ON550_000015"
     CDS             complement(14256..14567)
                     /locus_tag="ON550_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953134.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14748..16223
                     /locus_tag="ON550_000016"
     CDS             14748..16223
                     /locus_tag="ON550_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR3953135.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16270..16854
                     /locus_tag="ON550_000017"
     CDS             16270..16854
                     /locus_tag="ON550_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR3953136.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16856..17275
                     /locus_tag="ON550_000018"
     CDS             16856..17275
                     /locus_tag="ON550_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR3953137.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17556..17840)
                     /locus_tag="ON550_000019"
     CDS             complement(17556..17840)
                     /locus_tag="ON550_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR3953138.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18192..18902)
                     /locus_tag="ON550_000020"
     CDS             complement(18192..18902)
                     /locus_tag="ON550_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR3953139.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18899..20371)
                     /locus_tag="ON550_000021"
     CDS             complement(18899..20371)
                     /locus_tag="ON550_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3953140.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20509..20982
                     /locus_tag="ON550_000022"
     CDS             20509..20982
                     /locus_tag="ON550_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3953141.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21135..21686)
                     /locus_tag="ON550_000023"
     CDS             complement(21135..21686)
                     /locus_tag="ON550_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3953142.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21791..22321
                     /locus_tag="ON550_000024"
     CDS             21791..22321
                     /locus_tag="ON550_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR3953143.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22804..23247)
                     /locus_tag="ON550_000025"
     CDS             complement(22804..23247)
                     /locus_tag="ON550_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953144.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23263..24423)
                     /locus_tag="ON550_000026"
     CDS             complement(23263..24423)
                     /locus_tag="ON550_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3953145.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24930..26630
                     /locus_tag="ON550_000027"
     CDS             24930..26630
                     /locus_tag="ON550_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953146.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26732..27565)
                     /locus_tag="ON550_000028"
     CDS             complement(26732..27565)
                     /locus_tag="ON550_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3953147.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27869..30034)
                     /locus_tag="ON550_000029"
     CDS             complement(27869..30034)
                     /locus_tag="ON550_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3953148.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30294..32588
                     /locus_tag="ON550_000030"
     CDS             30294..32588
                     /locus_tag="ON550_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3953149.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32581..33246
                     /gene="pgmB"
                     /locus_tag="ON550_000031"
     CDS             32581..33246
                     /gene="pgmB"
                     /locus_tag="ON550_000031"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3953150.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33239..34246
                     /locus_tag="ON550_000032"
     CDS             33239..34246
                     /locus_tag="ON550_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3953151.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34268..35287
                     /locus_tag="ON550_000033"
     CDS             34268..35287
                     /locus_tag="ON550_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953152.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35568..36935)
                     /gene="nhaC"
                     /locus_tag="ON550_000034"
     CDS             complement(35568..36935)
                     /gene="nhaC"
                     /locus_tag="ON550_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3953153.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37044..38465)
                     /locus_tag="ON550_000035"
     CDS             complement(37044..38465)
                     /locus_tag="ON550_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3953154.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38708..40585)
                     /gene="tdc"
                     /locus_tag="ON550_000036"
     CDS             complement(38708..40585)
                     /gene="tdc"
                     /locus_tag="ON550_000036"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3953155.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40876..42132)
                     /locus_tag="ON550_000037"
     CDS             complement(40876..42132)
                     /locus_tag="ON550_000037"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR3953156.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42244..42449)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42712..44013
                     /locus_tag="ON550_000038"
     CDS             42712..44013
                     /locus_tag="ON550_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3953157.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44082..44681)
                     /locus_tag="ON550_000039"
     CDS             complement(44082..44681)
                     /locus_tag="ON550_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3953158.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44935..45816
                     /locus_tag="ON550_000040"
     CDS             44935..45816
                     /locus_tag="ON550_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953159.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45829..47439
                     /locus_tag="ON550_000041"
     CDS             45829..47439
                     /locus_tag="ON550_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR3953160.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47516..48175)
                     /locus_tag="ON550_000042"
     CDS             complement(47516..48175)
                     /locus_tag="ON550_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3953161.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(48458..49123)
                     /locus_tag="ON550_000043"
     CDS             complement(48458..49123)
                     /locus_tag="ON550_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR3953162.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(49270..49875)
                     /gene="dhaL"
                     /locus_tag="ON550_000044"
     CDS             complement(49270..49875)
                     /gene="dhaL"
                     /locus_tag="ON550_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR3953163.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(49903..50913)
                     /gene="dhaK"
                     /locus_tag="ON550_000045"
     CDS             complement(49903..50913)
                     /gene="dhaK"
                     /locus_tag="ON550_000045"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR3953164.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(50888..51334)
                     /gene="dhaM"
                     /locus_tag="ON550_000046"
     CDS             complement(50888..51334)
                     /gene="dhaM"
                     /locus_tag="ON550_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR3953165.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            51437..51757
                     /locus_tag="ON550_000047"
     CDS             51437..51757
                     /locus_tag="ON550_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR3953166.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(51869..52738)
                     /locus_tag="ON550_000048"
     CDS             complement(51869..52738)
                     /locus_tag="ON550_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3953167.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(52834..54138)
                     /locus_tag="ON550_000049"
     CDS             complement(52834..54138)
                     /locus_tag="ON550_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3953168.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(54135..54644)
                     /locus_tag="ON550_000050"
     CDS             complement(54135..54644)
                     /locus_tag="ON550_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953169.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(54819..56954)
                     /locus_tag="ON550_000051"
     CDS             complement(54819..56954)
                     /locus_tag="ON550_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3953170.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(57037..58692)
                     /locus_tag="ON550_000052"
     CDS             complement(57037..58692)
                     /locus_tag="ON550_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3953171.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(58694..59008)
                     /locus_tag="ON550_000053"
     CDS             complement(58694..59008)
                     /locus_tag="ON550_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3953172.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            59178..59438
                     /locus_tag="ON550_000054"
     CDS             59178..59438
                     /locus_tag="ON550_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR3953173.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            59452..59766
                     /locus_tag="ON550_000055"
     CDS             59452..59766
                     /locus_tag="ON550_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR3953174.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(60496..61050)
                     /locus_tag="ON550_000056"
     CDS             complement(60496..61050)
                     /locus_tag="ON550_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR3953175.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(61176..63105)
                     /locus_tag="ON550_000057"
                     /pseudo
     CDS             complement(61176..63105)
                     /locus_tag="ON550_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(63411..64127)
                     /locus_tag="ON550_000058"
     CDS             complement(63411..64127)
                     /locus_tag="ON550_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3953176.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(64128..65951)
                     /gene="glpO"
                     /locus_tag="ON550_000059"
     CDS             complement(64128..65951)
                     /gene="glpO"
                     /locus_tag="ON550_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR3953177.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(65965..67461)
                     /gene="glpK"
                     /locus_tag="ON550_000060"
     CDS             complement(65965..67461)
                     /gene="glpK"
                     /locus_tag="ON550_000060"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR3953178.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(67597..69057)
                     /locus_tag="ON550_000061"
     CDS             complement(67597..69057)
                     /locus_tag="ON550_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953179.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(69047..70342)
                     /locus_tag="ON550_000062"
     CDS             complement(69047..70342)
                     /locus_tag="ON550_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3953180.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(70674..71351)
                     /locus_tag="ON550_000063"
     CDS             complement(70674..71351)
                     /locus_tag="ON550_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR3953181.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(71381..72472)
                     /locus_tag="ON550_000064"
     CDS             complement(71381..72472)
                     /locus_tag="ON550_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR3953182.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(72573..73007)
                     /locus_tag="ON550_000065"
     CDS             complement(72573..73007)
                     /locus_tag="ON550_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3953183.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(73591..75048)
                     /gene="ascB"
                     /locus_tag="ON550_000066"
     CDS             complement(73591..75048)
                     /gene="ascB"
                     /locus_tag="ON550_000066"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3953184.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(75045..76940)
                     /locus_tag="ON550_000067"
     CDS             complement(75045..76940)
                     /locus_tag="ON550_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3953185.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(76998..78773)
                     /locus_tag="ON550_000068"
     CDS             complement(76998..78773)
                     /locus_tag="ON550_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3953186.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(78823..80319)
                     /locus_tag="ON550_000069"
     CDS             complement(78823..80319)
                     /locus_tag="ON550_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3953187.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(80513..82135)
                     /locus_tag="ON550_000070"
     CDS             complement(80513..82135)
                     /locus_tag="ON550_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3953188.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(82463..83776)
                     /locus_tag="ON550_000071"
     CDS             complement(82463..83776)
                     /locus_tag="ON550_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3953189.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(84021..84245)
                     /locus_tag="ON550_000072"
     CDS             complement(84021..84245)
                     /locus_tag="ON550_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953190.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(84265..84687)
                     /locus_tag="ON550_000073"
     CDS             complement(84265..84687)
                     /locus_tag="ON550_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3953191.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(84829..85539)
                     /locus_tag="ON550_000074"
     CDS             complement(84829..85539)
                     /locus_tag="ON550_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953192.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(85557..86054)
                     /locus_tag="ON550_000075"
     CDS             complement(85557..86054)
                     /locus_tag="ON550_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953193.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(86091..86543)
                     /locus_tag="ON550_000076"
     CDS             complement(86091..86543)
                     /locus_tag="ON550_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3953194.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(86678..87283)
                     /locus_tag="ON550_000077"
     CDS             complement(86678..87283)
                     /locus_tag="ON550_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3953195.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(87733..88353)
                     /locus_tag="ON550_000078"
     CDS             complement(87733..88353)
                     /locus_tag="ON550_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3953196.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(88355..89224)
                     /locus_tag="ON550_000079"
     CDS             complement(88355..89224)
                     /locus_tag="ON550_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR3953197.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(89247..89654)
                     /locus_tag="ON550_000080"
     CDS             complement(89247..89654)
                     /locus_tag="ON550_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3953198.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(89673..90962)
                     /locus_tag="ON550_000081"
     CDS             complement(89673..90962)
                     /locus_tag="ON550_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3953199.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        26616 a        19082 c        15820 g        29447 t
ORIGIN      
        1 aaaaggggat gtgacactgt cacactccct ttttaattta ttctatttct ttatatttat
       61 tttgtattcc gttacaaact cgcgtacttg gttaggttcc agagtgacat ctccaaaact
      121 ttcaagtcgc tccgctcctg gcagttcttg agtctcaaat gtgataccgc catgatttac
      181 taatttattc ccgcgcattt ccggtccatc ttctccaaaa tttgctgtaa aaatcacgat
      241 tcctgaagca tctgttttta cttggatcga tactttttca tctgggcttg tcaattccgc
      301 agcatttagt aaaccagatt gttttttcaa caaaaatgga tgatcgatgc cgcccactat
      361 cattgattgg cgaaaatcac tggcaaagac ctcacctaat tttttaggac gttggaagtc
      421 aaatgctgtg ccacttactg aaactacttc tcctgtaggc gttgtatctt tattgagcgt
      481 agcaaataat cggctattca accaaagtat atgttcgtcg attgactgtg ctggatcacc
      541 ggttaaattg aaatagacat gatttgtagg attgaatact gttttgcggt cactgatccc
      601 ttggaccgtc actcgccatg tatcatcgtt cgtcagcgta taccgtactt ctacatctaa
      661 agttccagga aatccgtttt ccatatctgg gctagtctga tagaaaatga ccgatgcttc
      721 gttttcacca ttgatgactg tataatgcca ctgcttggct tcaaagctag gtgcaccacc
      781 atgtaaacaa tgaccagtgt ccgggtctgt ttttacttcg aacgtttcat catctaattt
      841 gaatgtgcca ttcgtaatcc gtccagccat acgtccgatt gaagcaccga tataagggtc
      901 tttttctaga tattcttttg cttcatcaaa acctaagatc aactctcgtt tttcattatc
      961 gattattgtt tgaaaatgaa caacccgtgc cccaagatca ctgacagaca aagatgtccc
     1021 attttgattc gtgatcgtaa tcaagtgata gttttcgcca aaattttgtt cattgatttc
     1081 catgttcctt gcaccctctt ttctttcatt tgcactgatt ccagtataca tggaaaccgt
     1141 tttcttaaca gcttattccg tgagtaacga gaaaaaattt actatattct aataaaagac
     1201 cgtgaaaaaa aggttgtgac aaaaatattg tcacaacctc tttttccgaa caaacggtgt
     1261 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag gagctatttt
     1321 tgcttggctg ttcttattac ttgatgcagg acagcttttt cacaatctct ttttcaatac
     1381 gattccagta tataaccatg tcgtcagtac atagtagatg aaatacaata cgaagaatat
     1441 cgtaaacgga acccaaatat ctttgtatgg attttgcatg aatagaccaa acatttgtaa
     1501 tccgaataag acatggacta cacctaagat tcctggtgct aagaataaca cgcctacttc
     1561 tctacggatc gattgtttaa gcaacccgcg tcgtgttcct atcttttcaa gcatttcata
     1621 acgagcaatg tcactcttgc ttcctgacaa gatcttgaac atcaaacagc tagcaagcat
     1681 ggtcaagaaa gctaaaccta ggaagaatcc cataaattca aaaccagaga atagaccatt
     1741 atacacttca tacatttcat atttctggtt gctgtactct actttttgca aagatggatt
     1801 gttcatttta ttttgagcaa caagtgtttc gattggtttc aggtttgctt ggaagtcttt
     1861 tacctgaact agctgtaaag tggattgtgg caagttcagt tgatcaaatt ctgattgact
     1921 gactaccttg atttcttttt cttgctgttc cggcaattca tagcttcgaa gttcctcttg
     1981 tacagtcagt tcattcattt ttttagcagg aactttttct ttcgtgatct tcggcggtgt
     2041 tccatcttca tgttttatca tgatcaatgg ttgagccgca aattcacttg cattatagta
     2101 aatcgttccc gcatcttctt tttgagtata agaagcagaa agtgttggtg aaagttcttt
     2161 gattttttgc tgatcttctg cccgaggatt attcatcact agatcatatc cagaattgtt
     2221 ttctgtcaag tctgcaattt catttttaaa acctaatcct accgtcaaag cgcccagtgc
     2281 caaggcaaac agcatagcga ccattgagag catttgtgta tattcacgaa tacgaaagtt
     2341 taactgtgac aatgtgaaat tgttcaattt tttcaatgaa atctgttctg atttctttag
     2401 taatgataaa aagaaaatga tcacagaatg gaaaatgaaa taagttccta atacgatcgt
     2461 aactaaggct acccctatac caaagacttg ataggtcccc acgtgtttca tcatatagta
     2521 accgcagcct agaaaagcaa ttcccagtac tacttcaagg aaaaacaaga atttatgctg
     2581 cttgattctt gttggtgtct gatccgcacg aatcaatgct aaaatcggtt ttttgacaat
     2641 cgaaaaagca ttgacaacag cagctaataa aaacaaaaca gcaaaaaaca aaacagtgat
     2701 caacaacccg tgaatactaa aaggtgtaaa atgcgaaata ttgagatcta actgatccac
     2761 taataatcgg tggacgatac ttgtcaatcc tacaccgaca gctgaaccca ctaaagtagc
     2821 tgcgatccct actaagaatg tttcagaaaa aatcatctgt gcgatttttc tgccttttgc
     2881 tcccagcatc ataaacattg catagtcttt ttgccgcatc gtcatcaaaa aggaatttgc
     2941 atataaaata taaacgaatg tgatgatccc tagtaaaaca gaaccgaatc ggaaaatgat
     3001 aactgtagcc gcaacgatcg tattactttt caaaaaagct tcgttgcttg ccattgattc
     3061 aaacatgtaa aaaatagcag atgccataac taatcctgag aataacacca agtagtctct
     3121 taaacggctc ttgatcccag taagtgataa tttccaaagc atagtcaatc tccccctact
     3181 gttccaagtt tccaagaata gatagtattt ctcgatagaa ggaatcacgg ctttgatctc
     3241 cacgtttcac ttcttgatgg ataccgccat ccttgatgaa taaaatacgt tggcaataac
     3301 ttgcagaaaa cggatcatgc gtcacaagaa gaatcgatac atgatctttt gtattcaatt
     3361 cgtccatcgt atctaaaaga tcccgggcac ttttggaatc cagcgcacct gtaggctcat
     3421 cccctaatag aatcgttggt tgtgtgatca atgcacgtgc ggcggcgacc cgttgttttt
     3481 gtccaccgga aatctcagaa ggatagcttt ccaaaatatg agagatggat aaacgttctg
     3541 caattttttg gacacgttgc ttgatttctt ttggtctgac gccttgtaaa gaaagaggaa
     3601 cagcgatatt ttcttgtgct gtcaagcttt ctaataaatt gaaatcttgg aaaataaagc
     3661 caatctcatt cgcacgaaaa tctgctagtt gatttccttt taatgtggtc acatcttttt
     3721 gattgattcg gatatgccca tctgttggtt tatctaatgt ggataaaata ttcaaaagtg
     3781 tcgacttacc agaaccagaa gcgcccatga tcccaataaa ttcgcctttt tctacatcga
     3841 agctgatgcc gcttaatgct tgcgtctttt tctcattgtt ttttccgtat acttttgtta
     3901 cattctgtac ttcgactatt ttgttcattt ctttccctac cctttaatta gtttttccag
     3961 tgctgttttc ggcacgtctt ttttttcgac ttcttcatac gaagtgacac ctttattttt
     4021 gttccaggtg atttttaaat atgctccttt acgaagcggt cgagtcaatt gtcccttgaa
     4081 gtccagttct ttttccttac cctctttcgt atagccaggc aggatatatc tatagttgac
     4141 tgtttcttgt ccagagtctg attttgaaac tgtttttgta ccatctgtcg taatttgtac
     4201 atagtattct tctccaccca taactacgta atctgcgatt ttcacccctg caagtaccac
     4261 aacgatgaaa acaagtagac cgatcagaag ttttttcact ttttcccctt ctctcttact
     4321 ttatatactg attatagaaa aaagcgaaca attctgcctt cgaatcaact atcattcttc
     4381 tttcaatttt gtcattttct ttaaaacaac taaaaaaaga gttcaagaca ataatccgtc
     4441 ctaaactcct tcttcctatc ttacaaaaag tatgtcactt acggtctttc atgtcgcctg
     4501 tattgataac aggttgcgtt cctttgtctg taatgattga gacaagtaaa ttattgatca
     4561 attggacttt ccgatcatcg gtgaaattga tttcttgtcc atcttcgatt tgctctaagg
     4621 ccatttgagt gattgtgaca gctccttcaa caatggtttg tcgagcagac aaaatggctt
     4681 ttgcttgttg acgttggagc atggcactag cgatttctgt tgcataggct aaatggttca
     4741 agcgtgtttc gattacttcg acacctgcta ctgctaaacg ctcttgcagc tctttttgca
     4801 actcgtcaga cactgccgtc gtgtttcctc gaagcgtcaa atcattctca ttgaatgtat
     4861 catacgggta ttggctagcg atatgccgaa tggctgtctc actttggatt tctacaaaat
     4921 cttgataata cgctacatcg aataatgcct ttgctgtatc aacgacttta aagactacta
     4981 ctgctgagat ttcgatgggg tttccatcca aatcattgac ttttaaaaca gaactattga
     5041 aattccgaac tttcaacgaa acagtcattt tttgtgccaa aggtatcgta aggaaaaagc
     5101 cattttctcg aatcgtacct aaatattgtc caaaaaacaa aattactttt gcttgattag
     5161 gactgactac agttgccgaa cttaacaaca ataacgtgat caaccaaaga aaaatactta
     5221 aaaaaagtgc ccaaactttc gcttgccaca tacctaaata gaagaaaaac aatccaacta
     5281 aagctaatac gaccaatccg acaatccctg cataaccatt gatataaaat gctctttttt
     5341 ctctcatgct acttcctgcg ctcctttatc cttcaataaa tatctaatgt tactttttta
     5401 attttttttt cgaatacgcg ccattacacc ttctagttct aactctttgt cttcagtgat
     5461 gtaccaaccg cttgcctgtg tccctccggc tgcctgcggt aaataatcca attttgttcg
     5521 aacccagtgc ttttcttctg taaaatagtc ttctaccaag acctctagat ccatccctgc
     5581 tgaaagaggc aaatcttccc gaacagattt aaagataaat cgttccttct tcccatcata
     5641 cttcaatatt cctgtatcga taatcggtaa agaataataa tccagctctt tcttcaccgt
     5701 caataaatgt ttgaaaactt catggtacac ttgctctaat tggcgacctt ctgaattgtt
     5761 cgggtaatta accatcactt tttctttgtc tggatcatag aaggtttggc ttagttgagt
     5821 gatataagta tgtaatcttt ccatttctgt ttttaacttt tctctttgac cttcttctac
     5881 cttcatatac tctttcaccc tgttttcttt ttagatattt tctttcttat tttactatag
     5941 ttatgtgcgt taagcgatga aaaacagcaa agattggaag gatctttgct gttttttctt
     6001 aaaatatcca gttgagtaca ggatcaatct gtttcattgt cctcttgatc cgtcgtaata
     6061 ttgatcgatg tgtctttagc agtgatcttt agctttttct gtccttgttc taacaaagta
     6121 tttgttattg gctggtagtc tttttcaata taagattcac cagacgtttg gatagtatag
     6181 ccactgtttt ccatctcatg caaagataaa ttgccaccgt ttaggatgac ttttccttct
     6241 ttgctgatca aactgtcttc tagatggaag ttgctgtttt cacctgtgac gatcaagtta
     6301 tccacattgg cctcatctaa tcgaagtgag cctaattcat ctttcgcagt tattgttcct
     6361 cctgtaaatt cattgaacga tacccagcca tcatgtgtct tagtattcac ttttttgatt
     6421 gatacttcgt tgatatcaat tgcactatta ttcccttcga ttgtcaattc agataattca
     6481 tgatctgcag ggacaacaat agtgatttgt gcacttgtgt cttctaaact aatgattgca
     6541 cctgttgatt ttttgactgc attcacagtc aacacgccat ttttctcctt tatttctggc
     6601 ttttctgtct tatcataagt cgtttttatc tcaaaatcca atccgcgtat gaccgaaact
     6661 gtttgatttt ctgcatggat gacgattttg tttgcatcct tgattttttt tgtctcagta
     6721 accatatcga tcaaccgagg accattatcc caagtcagac tcttttgagc gcctaatgta
     6781 taagttgcac ctgctaggag gccaccagta agcatcagac caataccggt aatcaaaaaa
     6841 tgtttcaatc tcaatttatg tgtcctcctt caaacgcccg ctgaatgcag atctcttttt
     6901 gtaataaaac gtcgtcccat ccatttaaca aagttcatca tgacataaaa aagccctttt
     6961 gttcctttca gcaacagtgg cgaaataaat actgcgcttc ctgtggcgaa cagaccgaca
     7021 ccgataaaga ataatccact cagaatcgat tgtccaaaca cactgattcc tgagatgatc
     7081 atgaataggc caacacctaa gatactgata cataaaacat agatactaaa aatgagcgct
     7141 agtaaagcaa ccaatagtcc cccaataacc aatataaacc ccagtgctag tggaatgagg
     7201 acaggagaag caaataaacc gataatgatt aaccaaatca ggcgaatttg tctttttgga
     7261 tttttcacta actcgttttc tgcttgttct tcttctgaaa agtaactgat ttttaatttt
     7321 aaggcaaact gctttggtgt accatattca tcaatgatct gatctaatgt ttttccagaa
     7381 tctaaaaaat attcctcata atactggatc aaatcaaaac gatcgtcttc aggaatttct
     7441 tgaagatgat ccacaacttc attcaaatat tcattcattt gtgctctcct ccaataaccg
     7501 gtctaaagaa tctcgaaaaa caatccattc atcaataatt tcttctaaat gacgacttcc
     7561 cattggtgtc aacgagtaat atcgacggtt tctgccttca aaaggttcat cataggtctc
     7621 aagatatcct tcttttttca atcttctcaa aaccggatat agtgtagatt cactgacaga
     7681 gagactttct tgtacgattt ttgttaaggc atacccatac atatcttcct tgcttaatgc
     7741 tgctagcaca gtaccatcaa gaagaacagt tggaatctgg atgtgcatta cgtcaccttc
     7801 tattctcaag tgtaatattt tatttcaatc tatattatac aacatataat atagtttcaa
     7861 aatcagtttg tcaaatacaa aaaaactgac taaaagattt gatccttttt agtcagcaaa
     7921 aaatggcttc tctttgattt ttattataac agtggcataa tccaatttgt ccacatccag
     7981 ctcagtggca taataacgat cattgcaggt atcatgtcag ctactgggaa cattttcacg
     8041 ttgatcattc taaaaccggt agcgaccatc aagaatccgc cgcaagcttt aaaatctaag
     8101 atcatgtctg gtgtagtcaa tggataaata aattttgcca ataagaacaa aatataaaaa
     8161 ataatgaatt ggggaattgc tatcacagaa acgacatacc ctaaattaca cgcaaaaata
     8221 gctgctgtga aaaaatcaag gacagattta gaaatcaaaa tcgtactgtc tcctgtcatc
     8281 ccagaatcta aacttccata gatccccgta ccacttgcac aaaatagaac aatgaccgtc
     8341 actaatgtgc tgataaattc atcgtgggtc ataccaagct tttcactagg gaagatttta
     8401 gaaataggta cttgcatcag cgcagccccc ttatttatcc cattacctaa atgaacaatc
     8461 aaaccaatcg ctgttcctat cactaaagca aaaataacag ccggcatatt tttcatcggc
     8521 gcaatggaat agattcccat ccccatcgaa cacacaccaa aaaccatcgt cagctgtgct
     8581 ttaaaatgtt cacttaattt atttcctact aatccgccaa ttagtccgcc aaacaaaacg
     8641 gaacctacat tgatcaaaac tccagttggc atcaaaaaac tcctctcttt ttctacgaag
     8701 cgaaaaaaat cagaaaaatt tctgtaatta ttgctttcgc tgcaagaaag attgtaacag
     8761 acagagaaga ttacaccaat tcattttttg tgttaaagta ttctttattg gaataagtag
     8821 gaggaatatg atggatatcc ataaattcga agtttttctt gatttagccg aaacaatgaa
     8881 tttcaccaaa actgctgatc gacaattcac cacccaagga aatatttcta aacagattct
     8941 ttcgttagaa acagaattag acgtcaaatt attcgaacgc gcccatagaa aaatcgaatt
     9001 gaccgaagct ggaagcttat tgctcccata tgtaaaaaat gtcgtagaac aataccatgc
     9061 tatgcaagaa gtgcttgaaa cctatacgaa agacaacaat ctttcattaa atatcttaac
     9121 gataccgaca atgatcaatt ataaaggttt ttccaaaatc accgagtttt tgaaacgcca
     9181 tcctgaattt acagtccagt tgaaagaagt ggaaagtgtc gaattaactt acaatgacgc
     9241 caatgaacat agtgacacga tccactttgc tagaagtttc caggccccac caagttcgat
     9301 tgagtggttg cctactgaaa cagacgattt tgtagcagtt ctacctaaaa atcatccttt
     9361 agctagtaaa aagaaattgg atctaagcga actgaaaaag gagaactttt tgctccttgg
     9421 ccccactacg aacttgtatc aacctgtact agatctgtgt cgcgaagctg gatttgaacc
     9481 aaaaatctct tataaaggag caagaatcga cttgatcatc aatatgattg caaatgacat
     9541 ggggattgct attgttatgg aaaaaacagt caagaatcta ttaaaagaaa atactgtgat
     9601 tctccctatt tctccaacaa cagagagtta tcttgcattt actcgtaaag tcggtgagca
     9661 ttctttggct tcagatacat tttgggagta tttaaatcaa gaagaaaact aagccctaca
     9721 ttttctgtcc atctatccac ccaaaaaaag agagcgacgc atgactttcg ctctctttct
     9781 tgcatcatgt ccttgtttca atgacacctc taacatttct cgtagttcaa taagaaagca
     9841 gcgattttta tgccaaaaga accgtaagaa catttcccta atcacaaaaa aacgactaaa
     9901 aacccctaaa aaccttcaat agaattttta ggggttttat tctttttcct attgtttact
     9961 taaccgtatc attgctgtcg ttcctactcc ttcgatactg ctataatcaa tacgtccatc
    10021 atgcagttct agaatttttt taaccagtga aagtcctagc ccattcccag aaatttgacg
    10081 acttgtatct ccttgataaa atttttcaaa cattcgatct ctggtctctt cattcattcc
    10141 tacacctgaa tcggtcactt ccagtacaat ctctgtggct gtctcaaata gtttgatatg
    10201 aatctgtcca ttgatttgat tgtactttat ggcgttatcc atgatattca gccacacttg
    10261 gtacaaaaac tcttcattgc ctgtgtaatt tactgcagca agttcgatat ccaattctag
    10321 ctgttctttc tcccatttag gctgaaaaaa aacgattact tctctgattt gttcatctaa
    10381 tcgatactct ttcttttcca gttggatacg ctggttttcc agtttgttca attttaagat
    10441 gttctctgtt aattgtgaaa gctgcgtgat actttcgata atccgatata aaaatatttg
    10501 gcgttcttcg tcagagagat ttggggcttg taacaattga acatatcctt ggattgttgc
    10561 tagtggtgtt ttgaattcat gggatacact ggacacaaag tcattcctca gtgtctcgat
    10621 actattgagt tcttggacca taacgttgaa atctttatac agttgctgta cttctgctac
    10681 tttctgctgt tcatccattc gaatcgaaaa atctcccgtt gctaccagac tcatatttgt
    10741 ccctagcgca ctgattggat gcaggatacg ccgcccaaca aaaatcgata ttcctgtacc
    10801 cacgaccata ctgaaccccg caaagacgaa taacgggtat aacgttggtt ttccagccga
    10861 ttcagcaggc gagatagaat gtcgagacaa taagaaaata aaaccgagaa aacacagcag
    10921 tgtcactaag atcgaaatga agacaatact agcaaaatag atccataatt gtgaaaatag
    10981 cttttttttc atctttaaat aaccgcctta tagcctagtc cccgtactgt gatgatttga
    11041 aaatcttccg tcttttcaaa acgggtcctt aaacgtttga tatgtacatc gatcgttcgt
    11101 tcttctgtat tcgtatctaa tccccaaata tcatccatca actgttggcg agtgaagatc
    11161 ttgttgggat aagacagtaa tttatacaga agcagaaatt ctttttgagg cagaatcaac
    11221 gaatgctgat cttgtaacac tgtataagca tctagctcta attttgtttg tccgattatc
    11281 aagctactgc tgtaattgat ctttgaacgt cgaagcaatg cttccacacg taggatcatc
    11341 tcattgacat cgatgggttt tgtcatataa tcatctgctc ctaatttgaa cccgcgtttc
    11401 ttatcctcaa aatcggcctt tgccgtaatc attagaacag gtgtgtcaaa tctggtgcta
    11461 cgtaatgagt ctagcaagtc atagccatcc attttcggca tcatgatatc tgtaatgatt
    11521 aattctatac gtgtcgcttc tagttttttc agcgcatcct ctccattcaa tgcggtgatg
    11581 actgaaaagc ccgctcgttc caacacagaa cggtaaagca actgtaattg cggatcatct
    11641 tctactacta aaatacgatt cattgatctc cctctgtttt ttgaaaaatt tctagatttt
    11701 ttcttgtagt tcatcttcag ttaacattta tttcgtatac tacgatcatt gttctgatat
    11761 acttattagt ataccgaata tttcaatacg ggagaagggt gattatgctt caattaaaag
    11821 atataaaaaa atattataaa gtcggcgaaa ccaccaccaa agcattggat ggtgtgtctg
    11881 tcgctttcag acaaaaagaa ttcgtagcga ttttaggtcc tagtggttct ggtaaaacga
    11941 ctatgctgaa tgtgattggt ggactagata attatgattc cggtgatatg gtaatcaacg
    12001 gaaagtctac taaagacttt aaggacagtg actgggatgc ctaccgaaac aactctatcg
    12061 gtttcatttt tcaaagttac aacttgattg gtcatctagg cattattgaa aacgttgaac
    12121 ttgggatgac attaagtggt gtttctaaag acgaaaaacg aaaacgggca gaagatgcat
    12181 tgcaccgagt cggcttgaca aatcatatgc ataaaaagcc aaatcagtta tctggtggac
    12241 aaatgcagcg agtggccatc gctcgagcac tagccaatga cccagatatc cttttgtgcg
    12301 atgaaccaac cggagcgtta gatacagaaa caagtatcca gatcatggaa ttgatccaag
    12361 agttatcgaa agaaaaatta gtgatcatgg tcacacataa tcctgaactg gcccatcaat
    12421 acgcagatcg aatcattgaa ttttctgatg gtaagatctt aacagattcc catcctcaca
    12481 tcgaacgacc aaaagatgat caatttaact tacgcagaac aaagatgagt ttttggacag
    12541 cgttaaagct ttccttcaac aatatccgaa caaaaaaagg acgtactttc ttgacctcct
    12601 ttgcttccag tattgggatc atcggaatcg cgatcgttct gtccctttct tctggattcc
    12661 aaaaacaaat cgataacacg caagctgaaa cgatggcgaa atttccgatt actatttcta
    12721 aggtaacgac taaccagact cgtgacgatg ccggattggg tgcaagcaaa gcggattatc
    12781 cagacagcaa aacgattact gcaaaagtca gcgaagaaga taaagcacag cacacgaata
    12841 agattgatca aacatacgtc gattacgtaa cagacatcga tcctaactta agtaataata
    12901 tcggatttac ccgtactaca ggaatcaatc ttttacgtga tgtaaatggc aaagtacagc
    12961 ctgtcagctt ctcgaatcaa aatcctgata cggaatctct ttctttatct agtacgatgt
    13021 cagcaatgac aggtgtggga gtgtcaagtt tccctacaca gcttgacact agcaaagaaa
    13081 actttttaaa agacaattat tctttacttg ccggaagtta tccagcctca gcaacagatg
    13141 tcgtgctgat cgtagacggc aataataata ctaatatcaa cgcattgaag aatcttggat
    13201 ttgatgtgaa agaagacgaa aaacttgatt ttgatgatat cgttggtaca acattcaaat
    13261 tagtaaacaa caatacttac tatacaaaac ttccaactgg gaattttatc cctaatacag
    13321 attatgatgc gatgtatcag aatgcttctg atgaattgaa gatctcgggg atcctacgcg
    13381 tgaaatcttc ttctacaatg aatctcttat ctccaggaat cgcttatagc gatcaattaa
    13441 cgactcaaat cgtcaacgaa aacaaagaat cagaaatcgt caaagctcaa agagacagtg
    13501 gtgtaaatgt cttaactact gaaaaagttg acgaaaatgc caaacaaaca ttgcttagtt
    13561 atttaggtgg agacagcttg ccttcaagta tcatgattta tccgaacaat tttgaagaca
    13621 aagaaaagat cttggattat ctagatgatt acaataaagg caaatcagat gaagataaaa
    13681 tcatttatac tgatttagct ggaacgatga ctgaactgac tggcggattg atggatgcta
    13741 tcacttatgt ccttattgct tttgcaggga tctctttagt aacaagtatg atcatgatca
    13801 gtatcattac ttatacatct gtgattgaac ggacaaaaga aatcggtgta ctaaaagctt
    13861 taggtgcacg taaaaaagac atcactcgcg tatttgacgc agaaacgtgt attttaggta
    13921 tttcttctgg gatattaggt gtgtttattg catggctagc tacgttccct atcaatagca
    13981 tcttatacaa catgacggat ctaaaaaatg ttgctcaact aaatcctgtc catgcgatta
    14041 ttctagtgat cgtctctact atattgacga tgcttggcgg tcatcttcct gcacgaatgg
    14101 cagcgaaaaa agacgccgct atcgctttgc gtgcagaata aggttgtgac aaccacattt
    14161 agatttgagt cataagagcg ctgaaatagt agaaaacagc aagaaacgag gaagtaatat
    14221 gttccttgtt tcgcgctgtt ttttttaatt attttttata attctggtat acttttcttt
    14281 cctttgtacc acaactcttc tggcgtatac ctgtcttttc catttatgaa tactgttcca
    14341 tctgcttcaa tcgttacttc agtcccaatc cacatattac gagaaagtgc gagaagattt
    14401 tttctttttc ctcttccttt ttttccaaat tgcaaggcat cggttatctc ttctgtccaa
    14461 tgagatttca cgtattttac ttctccgttt tcaaatgtaa ctttcaaaat attcgctata
    14521 ttttccactt ttactgcttt aggaaatgac tcctccattt ttttcacttt ccttcctcca
    14581 acactaagta tgcgctttct tttagtgata agtatatcat agtttattct attatcaaaa
    14641 aaacgcatcc attccattgt agaatacttt accgaacaag ttgaattgtt ttcctctttt
    14701 tcacctttta ctatagatag gtaaagacaa ctaaggagga atctctcatg acagatcaac
    14761 cgtatgattt aagaaaagtc atcgaagaac taaaaacact tccaggtcaa tatcacgaaa
    14821 cagatattga aatcgatcct gaagcagaat tatctggtgt ttaccgttat atcggtgctg
    14881 gcggtacagt aaaacgtccg actcaagaag gaccagctat gactttcaac aatatcaaag
    14941 gtttccccgg cacacgtgtg aacatcggaa caatggctag tcgtaaacgt gtaggtgaaa
    15001 ttttgcacca tgattatcga acacttggtc gtttcttgaa agacgctgta gaaaatccaa
    15061 tcaaacctgt gaaagtagac aaagcagacg ctccagcaca agaagtcgtt catctagcta
    15121 ccgatgcaga cttcgatatt cgcaacattt tgcctgcacc tacaaatact cctgaagatg
    15181 ctggtccata catcacaatg ggtgtagtct taggatcaga tccagaaaaa acaatgagcg
    15241 atgtaacgat ccatcgtatg gttctagaag ataaagatac gatcggtatg tacatcatgc
    15301 ctggcggacg ccacattggt cacttccaaa aacaattcga agccttgaat aaaccaatgc
    15361 ctatcactat caatattggt ttagatccag caattgccat tggcacgact ttcgaaccac
    15421 ctaccactcc acttggctac aatgaactat ggtgtgcagg agctttgcgt aacgaaccta
    15481 ttcaaatcgt tgatggtata gcagtcgatg aagtgggtat tgctcgtgct gaattcatca
    15541 ttgaagcaga aatcatgcca aatgaaacaa tgcaagaaga tatcaacacg aactcaggaa
    15601 aagcaatgcc agaatttcct ggctatactg gggatgctaa tccagctgta caagtagtga
    15661 aagtcaaagc agttactcac cgaaaagaca atccaatcat gcaaacaacg attggtccca
    15721 gtgaagaaca cgtatctatg gccggtattc ctacagaagc aagtattctt gccttggtcg
    15781 ataaagctat tccaggaaaa gtagtaaatg tctacaatcc tcctgcaggt ggtggtaaat
    15841 tgatgaccat catgcaaatc cgtaaagaaa acgaagcgga tgaaggtatt cagcgtcaag
    15901 cagctattct tgctttatca gccttcaaag aattgaagac agtcatttta gtcgatgaag
    15961 atgttgatat tttcgacatg aacgatgtag tttggacttt caacactcgt ttccaaggag
    16021 ataaagatat catcgtgatc ccaggaatga ggaaccatcc attggaccca tcagaattac
    16081 cagaatataa tcctggctct attcgtttcc gtgggataag tgcaaaaact atctttgacg
    16141 gtacagttcc tttcgatatg aaagaacatt ttgtacgtgc aaaattccaa gaagtagaag
    16201 attgggaaaa atatttgaaa taatgaatga tctgcaactg ttttgacatc ataaaaagga
    16261 gtgcggaaaa tggaacaatt ggcacaacgg aagaaaaaaa tcgtgattgg tgtaagtggc
    16321 gcttcaggca ccatttatgc catcaaccta gtgaagaaac taaaagaata tccgatgata
    16381 gaaacacacg gaatcatcag tccatgggca aaacagaact taaagctgga atccgactta
    16441 tcactaactg aattcgaaag cctgtttgat taccattatt caaacaaaga tttaggtgct
    16501 gctgtcgcca gcggatcatt tttgaccgat ggaatgatca tcgtaccggc aagtatgaaa
    16561 acagtcgcag gtatctctgt tggtttaggc gacaacctga ttgcacgtgc tgctgatgta
    16621 acaatgaagg agcaacgaaa gttagtcatt gttcctcgtg aaacgccatt aaatgccatt
    16681 cacttggaaa acttgactaa attagcacga ctaggtgtgc aaatcatacc accaattcct
    16741 gctttttata atcagcctca aacgatccaa gatcttgtta atcaccatac aatgaaactt
    16801 ttggatcaat ttgaaattgt aaatgagcat tcaaaacgtt ggcagggtga ttaatatgga
    16861 aaaagaaacg atagttgaag taaccaaact aggtttcacg atccaagctg ctgttaaagt
    16921 aatcggtaaa gacttattga tcactttaac aggcggagat acaccacata tcggaacagt
    16981 cactacattt tctcgtgata cagagatgca aactgtacgt ttccctagcc acgatggtcg
    17041 gttccataaa gacgatgtct tagccgaaaa aatagctgtg atcatcaaaa ctgccgttcc
    17101 gggaagttgt acgatcactt ccggtatcca tgttgaccat atctccaatg cacaaatcaa
    17161 cgcctctttt ccgatgtcag aagagttagg ttacaagatt ttagattggt tggaaaaaat
    17221 agatttccat atcaaagaac cagtttatta taaagatggt gaaaaaccat tatgaaagaa
    17281 gaggttgtga aatcagcgtt atgacctgag tatgagagaa aaaaatggaa aaatagcttc
    17341 tcatattttg ccgtttttga gctaataccg agggacagct gattgaacgc cgtttatcaa
    17401 gaggttgtga taaagccgct tcgtttcgag tagtaagaaa aattttgtaa acatgggttt
    17461 ccacattttt gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc
    17521 aagaggttgt gacacgtctt ttgtcacaac ctcttttatt tttgcttcac tgtaactgct
    17581 acaatatcac cgatttcttt tccgatgttt ttacgaatat cttttcgtag tccaaggatg
    17641 tagcagactg aaccatctgg atttttcacg cccatattca cgatactgcc ttcatacggc
    17701 acattgtcaa aagttgcttg gactttaact cttccagcac cgaattcttt tcttatatca
    17761 tacggaaaaa taatatatgc cccgcccttg ccgacttcgg aagcatagat ttttgattca
    17821 aaagaataaa tttccctcat tttatttctc ctatttttta catctagtat agcatagcaa
    17881 aaaaaagaag gaactgtgac aaaagtcttg tcgcagttcc ttttttcgaa taaacggtgt
    17941 tctcctgcca actcctcggt cacaagtcac aatagtcaac aaacatgaga agctgttttt
    18001 tgaaattgct ccttgtgact cagagttaaa cggaaggctc acaacctctt ccttaaacgg
    18061 tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat
    18121 ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc tcctacatgg
    18181 tgttcaaaat attattcgcc ttttgcttct ttcactaatt ttgcgaatgc ttgttgttgg
    18241 aattcagcag ctaaggcttt cgtacgttgt tcttgctctg ctttgctcat ttttggattt
    18301 tcagtcattg cttttaccat accgtcttga acataagctt ttgcttcact ttccatcgta
    18361 ctttgctgtt tcttcactaa ttcagctaac ttggctgctt ggtccgtact taattctacc
    18421 catgtctctg ggattttgaa tgtattcact cggctgtcat attcgtgatt gttatcagca
    18481 atattgaacc agaatttata catgtcgttt ttgtattccc aataagtagt tttcgtcact
    18541 ttttcagcat cgccgtctgt cttttctact ttatttgttt cgttatccgt accagttgct
    18601 tttggttttt cttgtttttc atttgttcgg taaagataca ctttttcttt tccattccct
    18661 aaagctttat acaaaagcat atctgctcct tgggaatcag cactggaaac aattttttct
    18721 gtttttgttt ccgttacttt ttccattcca aagtgattgg acaaattagc gacgatcaaa
    18781 cttagtgaag cgacgaagat tagtccgaaa atcaaagata aaaaggtttg ccatgttttt
    18841 ttagcgaaca cgttcgttaa agcaaaagca agcaagctga caatcaaaac tccaataatc
    18901 atgcattatc ccccgctttc attagaacag gttctttttt cttcaataaa aaggcaataa
    18961 taaagccaag aatcccaaag atgatcgcta cgataaacgc ggcatggtaa cctgacagtg
    19021 tggcattgat tgcctgatct ttataagcta atggcgtatt atccaataca gatttgcttg
    19081 ggatatttgc ttttgtcaca ttcgtcaaca cactgatcaa aatagctgta ccaatcgagc
    19141 ttgcgatttg acggaatgta ttatttaccg ctgtcccatg actgatcaag ttatttggca
    19201 atgcattcat accagaagtc gttaccggca tcatgaccat ggagatccca aacatacgga
    19261 tcgcataaag gatcacgata tataaaactg gagtcgtttt tgtcaagaaa gcaaatggtg
    19321 cagttgccgc tgtcaaaatg aacatccctg tgatggctaa acgtttcgcg ccgtatttat
    19381 cgaaaatcgc acccgtcaca ggcatcatag cccccatgat caatgcaccc ggcaacaaca
    19441 tcaagccaga atggaatgct gattctccac gaatattttg aatatacaat ggtacgacca
    19501 tttctgcccc taccatcgcc atatttgtta caccagctaa aacagctgca atcgtgaatg
    19561 tagatgattt gaatacacga agttccaaga atggatgttc caaatgtaat tgacgccatg
    19621 agaacaaggc gatgaaaatc acaccaacaa ctaagaaacc aattactttc aagcttcccc
    19681 agccgtcatc tcctacacta gaaaatccgt aaagtaaact accgaatcca atcgttgata
    19741 aaatagcgga taaaatatca atgctaggat ttgacaatgg aataacactt ttcattagga
    19801 agaatgctaa aaccaagaca agaacaacga ttggcagtac cataccaaac aagtcacgcc
    19861 aatggtagtt atcaatgata taaccagata atgtagggcc taaagcagga gcaagtccaa
    19921 taacgatccc tgacatcccc attgctgatc ctcttttttc tggcgggaaa atagatagca
    19981 tgatattttg caataatggc atcgatactc ccacaccgca agcttggatc aaacgaccga
    20041 ttagtaatgt actaaaatta ggtgctgtaa agcaagtaat cgttccgatc aaaaagatcg
    20101 tcatggcaga aagatataaa tttcttgaac tgaatttatt gatcagccat gctgttattg
    20161 ggatcatgat cccgttgact agcaagaaac cagtcgttaa ccattgcaca tcagacgctg
    20221 aaatatcgaa agctttcatc aatgtaggaa aagctgtcgt caataaggtc tgattcaaaa
    20281 cggtacaaaa tgtaccaatt aataagacaa caactaaaag tgtccgatta tatggttttc
    20341 cgtaaatatc tacgggttgg ttgttactca tatttctcct tctttctgaa ttttttctga
    20401 aactactaga atactttagt cctaaattcg ctatttgtca acctacttga cacataagtt
    20461 aatacggata taattaaggg aagcaagagg acaaaaggag tgacaaaaat ggttggggct
    20521 tcccttaaac gtttgttgga aaatgatcaa ttggttgttg gcatcagtga attaagtgat
    20581 atcgtaggtg tgtctccacg tcaattaagg tattgggaac aaaagggatt cattcgttcg
    20641 atcgaagctg atgcgaattg tcctagaaaa tatcgtttac ctacagtcat aaaagtagaa
    20701 ctgatcaaaa aatatttaga tgacggattt actttgtcaa aagctgcaga aaaggcagaa
    20761 atccgccaaa agaaaatgca ccatgtccgt aaagtttttt caaaagcaat caaagatatc
    20821 gaattgatcg acgaacgcta tacgatttta caaatcggta acttcaaaga agagaatgct
    20881 aagatgtaca tcattcatga caacgagaca gacgaattaa cttacaagct ccttcctgtc
    20941 gatgaaaaag ctgactacga acagttgtta aaagaactct aaacgccact aatctcctaa
    21001 gtaccaagct ttttaataag tagatgctac ataaatactt gaaaaaaggc ctgtttgaac
    21061 ataagaatat ccgaacggtg atctgcgttt ctttcatata gaaaggaatt ctccatcact
    21121 gttcggatat ttttttattt ttttcggtta ctttcttcta atacatctgt tagccaattt
    21181 gcatattctg tttcccgatt gatcgcatgc gctaaaatca ggtagtgtcc gtaattttca
    21241 tgtttttctt tttcagtagg aaagatcgtt ttttttcgct ctaatagatg aagtcgtttt
    21301 tcttcatgaa ggtcacgctg ctgctgaata atttctacta atgccggatc attcacgtct
    21361 ttgacgaaat acaatttcag aacaaattcg tctcgattaa ccggtagttc attggtgggt
    21421 gtatgaatcc actctgccaa ttgttccttc ccttcttctg ttaattcata aactttcttt
    21481 tccaattttt cacctgtgat ctctacttga tgctgaatga ttccttgctc ttccattttt
    21541 gcaagttcag gataaatctg actatgtttt gcttgccaaa attctcctat ttcatttttg
    21601 aacgattgat tcaattcata tcctgtcatc ttcttcttac ttaataaccc taacaaaata
    21661 tatcgaagtg tattttttct tgccatcagt tttccctcct tgacaaatct ttaaatccat
    21721 tctatcataa atgagtaatc gaaaacatgt agtttattac atgttttaat ttataaggag
    21781 atgcttaaac atgaaagaat ttaaagaatt ggaagatttt attggaactc actttatcta
    21841 tacgtatgac aatggttggg aatatgaatg gtatgccaaa aatgaccata ctgttgatta
    21901 ccgtatccat ggcggaatgg tcaaaggccg ttgggtaaaa gaccaagaag ctgatatcgt
    21961 taaattgacg gacggcgtat tcaaaatcac ttggacagag ccaacaggga cagacgtggc
    22021 attggacttt atgccaaatg aaaagaaact tcacggaatg atctttttcc ctaaatgggt
    22081 tgaagaacat ccagaaatca ctgttacttt ccaaaacgaa catatcgcag aaatggaagc
    22141 agctcgtgaa aaatatgaaa cttatccaaa attacttgtt cctgaatttg ctacgatcac
    22201 ttatatgggt gatgcaggta tagacaatga agatgttatt agcgaagaac catacgatgg
    22261 aatgccagat gatatccgta gcggaaaata cttcgatgaa aattataaac gagtaaaata
    22321 aggttgtgaa agagaggttg tgaaatcagc gttctgacct gagtattaga gaaagaaacg
    22381 gaaaaatagt tcctcatgtt ttgccgtttt ttgagctaat accgagggtc agctgattga
    22441 acaccgttta tcaagaggtt gtgaaatcag cgttatgctt tgagtaataa gaacgctagt
    22501 tagaaaatag cttttcatat tttctaggct agtgggctta ttaccgagaa gcagccgttt
    22561 gaacaccgtt tatcaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    22621 atggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    22681 tgaacaccgt ttatcaaaga ttgtaacaac tgcatattca cacagttaaa ttagtgactt
    22741 caaaatgaaa taatgctccc agcaaaaaat taggctggga gcattatttc gttattctgt
    22801 tcatcaaaaa tcagcgtttt gattccattt catcacaagt tcctcctcat ttactgcttc
    22861 atctttttgc tgtgtttgaa ttgtaaaacc ttgtgaaaga tagaaatgaa tagctgattg
    22921 atttttttga tagactgtca aagtcagtgt tttattggtt tcttttattt ttttcattag
    22981 ttgcgtaccg attctttgtt gccggtattc ccttttcaca aagattcctg cgataaactg
    23041 atcaatcacc ccagcaaaac caataatttc ttgatctatt tctgctaagt aaatcactgc
    23101 atctggtaaa gctttcctaa cagattctac ttgattctcc caataagttg atggaatgaa
    23161 ttcatgagaa tccaagttag catgcagcca aatcgataga acagattcca gttctttggc
    23221 agacaatttt gttgttgtgc ggatcatttt ttctctcctt acttattcat tgattttaaa
    23281 ataaaaacca tttttttgaa gctcttttac aagttttcgc ccctcaaaaa agtatatctt
    23341 ctcttgtttt acttttctaa taccaaaaga aacagcaact ttaggatcat cagctgaaac
    23401 gattcgataa gttccaccag gcaattgatc cagcttgact ttcccatgat ggtcgctttg
    23461 tactgaaaac ggcacaccat ttctagtaag tgtttttttc ttatgtttca aaatgaatga
    23521 actaccattt accggtttcc ctgacgaatc actaataata aatgaaaaat caagcacttt
    23581 tttacttaat ctatactgat agattagtcg tccgataaac actaacagta tgactacagc
    23641 tattatcgtt cctgcaagaa taaaccattg ccgcatggtt ctttcctgat ttccttttcc
    23701 ttcttctttt tttaccgatt cgttataagg aatccgatgt cctgtcacta gcaaacgatg
    23761 cgtgttgatc atatacggcg tacatgtaag caaggtaact aagtcttctc cttctgaaat
    23821 cgttagaaca gacgtgtcat ctggcagtac cacttgtata tttttaactt gataagccaa
    23881 tttctccccc aaaacagtca aaataaacgt gtctccgttt tctaaacgat ctaattcggt
    23941 aaataaccgt cgattaggta aaccgctgtg agcagagatc actgaatgcg tcgaatttcc
    24001 accaagcggg aaagaggttc cttgcaagac gcctgctcca ttttcaagga tcctaccatt
    24061 gagccgatca taaagtgaaa tatttaattg gatcttaggt atattgatac ttccgatcag
    24121 ccattcctct aattggctgg atgttgtact ttcatttctg ttcttttcat tgaaaggatc
    24181 agtaccagga ttgaatcctt ggtcagttaa tcgttggttt ttctcctcca ttttctttcg
    24241 ctgcacttca atttcagctg cggtccgttt ttgtacttgt tcgatacgtt tttgatcaat
    24301 aaaactattt aaagaatcca cataaaatgg atataaagcg atcagtaacc ctacgacgat
    24361 acatattaat aaaaacaaac gaaacaaaag tcgacgagac tgtttttgct tactctgctt
    24421 cacatgtgtc ctcccttgca agtttttttg ttctttcttt tattatatgg gttcaggaaa
    24481 aaaatagaaa atgaaagccg atgaggttgt gaaaaagctg tcctgcatca agtaataagt
    24541 acagcccaaa aaaatagcta ctcatatttt ttgttgggtt gggcttatta ccgcagatgc
    24601 aagcttttga acaccgttta aggaagaggt tgtgaaaact gcgttatgct ttgagtaata
    24661 agagcgctag tcagaaaata gcttctcata ttttctaggc tagtgagctt attaccgaga
    24721 agcagctgat tgaacaccgt ttaccgagag gtcatgaaaa agctactttg tttcgagtat
    24781 taagaaaaat tctgtttcaa aacaaggaga tgcaacaaaa cttatgtcgc atcctctctc
    24841 cttagaactt tcacttatgt ttttcttgta aaattcataa atattttcat gaattttcta
    24901 atttcttgcc tacctctcgc ataggatata tgatgaattt atctaaagtt tgcaaaggag
    24961 ttcgacaatt gaaaaaatat ggcccatttt tagcaatttt tttcattgct ttggcatttc
    25021 tcatgcctca tttattcaca cataaactaa ttttgggtgc tgatgcccca ttccattaca
    25081 atcggttcta tgatgccgcg caacaaatta aatatgggga tttccattat tttccttccc
    25141 tttatggatt ccagcaaagt ggcaggatcg tgaatgccgt ttacggccca tttttcgctt
    25201 atttacaagg attgatcgtg ttgcttgcag gtagctggtt tcattatcag attctttcaa
    25261 atttcattct ttatcttatt tcaggatttt ctatgctgat tttactgaga tatgtcaatg
    25321 ttcgaaattg ggtaagtgta ccagtagccg ttttattcat gagtacgtat tctattcaat
    25381 actggtctat caaccaagga ttcactagct gggggacttg tttttatcct ttatgtatga
    25441 tccctattgc tgatttcgtc cttcataaaa agttccctgt tatcaaagtc gctgtctcag
    25501 tgggacttat gacacagatc catttactgt cttcgatcat gttagctttg atgtatatcc
    25561 ctttttatct ttattacttt tttaaccaga ctgaaaaagg aaaagcactg cttcaactga
    25621 ttaagtcggt cttgctttat tttgttctga cagcaaatat ttgggcagga ttagctattg
    25681 tttacagtgg gaataagatt cttcctccct ttgttaacag agaaatgagt caaaaggcga
    25741 tagatctgga tggcagttat tggctccatt accctaaggc ctttcctata ttgatattat
    25801 caggtttttt gctttttatt ttctttcaac aaaagaacac cttattccaa aaaatcgtgt
    25861 tgtacacaag tcttgttttc ttttttcttt ctactagctt gattccatgg acgacattga
    25921 tcgaaaaaaa tgtgcctttt attagtttga tccagtttcc atttcgattt ttcgtgccat
    25981 ttacgctatt gcttctactt taccttgccc tttcactgaa ccagtggtct gaacaaaagt
    26041 ggttccgtct tttaagtata tttggcattc ttattttttc agcacagacg atccagaact
    26101 tataccagca tctggaaaag tgggaaaatg aaacatttgt cagtcgacat acttatctat
    26161 tcgatacgcc ggaagaagca aggaaaacat tcttcagcaa agatatgtct accagcttgt
    26221 tcgttttcca aaaaaccaca cctgactatc tgccaatcta taaggagaca aaagacaata
    26281 aatacgaccg gtataacgaa gaaatactag aaaacgaaac aacttttata aaaacacatc
    26341 caggcggtct tttgacaatc aagtggaaaa acactgggga caaaactgtt catatccctg
    26401 ttatcgtcta cgatcggact gtgctacagc aaaatggaaa aacattgact gattatgaag
    26461 taacagatat cgggacccca atcgtgaagc agcaaaaagg aatcaacgag ctcacgcttc
    26521 attatcaaac gccaatctat ttctatttca ttctttctct tactttgatt ggctggttta
    26581 ccttgctatg tttatttatt tatcatagat acaagctttt acgtgcttag gcaaaaggga
    26641 taaatacatc cgatcaaaaa actctccaaa cggtataatc gtttggagag ttttatttta
    26701 atcagttttt ttatgttata tgttatattt tttattcgtt cacatctaat tcgatttcca
    26761 ccccgtagtg atcactgatg attggactat cgatcccatc aaaaatccgt tgatattttt
    26821 taacctgcat tccttcaggg acgaaaataa aatctatacg caatttttct gtattttgtt
    26881 cccaaccatc tattttcttt tccacagtag ctgcttttcc tttttcattc gcgacaacat
    26941 acgaatcttg aagaagaagg caactttctc ttacagtata ataaccttga gagtctactg
    27001 gattattgaa atcacctagg aaaaaaagtg gtacgcgagt ttccaaaaag tagttttcca
    27061 agtttttcca ctcaaaataa aagccacttt ttttatctgt ccaccaagaa aagtggcagc
    27121 tcgagacaaa aatgtcctga tcatctatct ttgttttccc aatcaagatc ttgcgtgtct
    27181 gataatcttc tttcttttga aattgggaga cggtaaggac ctgactttcc aatgtttttt
    27241 tcgtaagcag tgcatttcct tcttcatatt tatcataacc gatgtggctc atttcccaac
    27301 tccaaaaata ctcttttccg tgttccttta aaagctgaac taaacggtag gcaaaattat
    27361 ctttacggat aggtacttga ttctttaccg gacagaactt cattagcttc gcaggttcta
    27421 cttcttcaga ttccagtaat tggttgattt cttgtaaagc aatcacttca taatcttcta
    27481 acaggatctt atctgctaat tcttgcaatt ttttctctgg ttctggctca agccagctat
    27541 gcgtgttgag tgtcatgaat ttcattggtt ttctccttgt cttcatttcg aaacaaaagg
    27601 gttgtgacaa aatttttgtc acaacctctc cagcaaatag tgttctcctg ctaactcctc
    27661 agtaataagc tcaatggacc aggagctatt ttttgtttgg ctgtttttat cacttggtgc
    27721 agggcagctt tttcacgacc tctccactaa acagtattca aactgctgct tctcggtaat
    27781 aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tatgacttga
    27841 tgcaggacag ctttttcaca acctctcctt acattttaat ggagccgatc actttaccag
    27901 ctgtcactgt acctaattgc ttgatcacta cttcttctac tttatcgttt gtaaatacga
    27961 tcatgatcgt tggatctttt tcttcgtttt ggatcacatc aagatccatc tcagctaatt
    28021 tcgttcctgt tcctacttct tggccttctt tgacagcaat ctcaaaagct ggctgattca
    28081 tttggactgt atcgattccc atatggatca aaacttcgat accatctttc gtttccagcc
    28141 caattgcatg tttgctaggg aaaatacttg tcacttttcc agcgatcggc gcaacaactt
    28201 ctcttgatgt tggaataaca gcaaagccgt cacccatcat tttttgtgag aataccggat
    28261 cttttacttc agataaagga atcacttctc cagaagctgc ggaaactagt tcttgttcta
    28321 tacctaagta ttgcttacta cctgcttgtc catttcctgt agaaaccatt tctggctctg
    28381 gaatctctgc tcctgattgc agcagatctt cgatatctga tttcaagaca tctgcttttg
    28441 gtccataaac tgcttggact ccattgtctt tgacgatcaa tcccatcgcc cctgctcgtt
    28501 tccaggcttc ttcagtccct actttttctc gatcatttac acttacgcgt aaccgagtca
    28561 tacaagcatc gacatctttg atattttctt ttcctcccag caaatgaatg atctggacca
    28621 cttgctgatc tgctgttcct gttccgctgt tagatacaac ggtttcttca ctgtcattgt
    28681 cgtaatttcc gttacgtccc ggtgtagcaa agttgaattt tttgatcaag aaatttgcta
    28741 agaaatacgt aacaacacca aaagcgatca cacacaacac gaagttgata agatctccac
    28801 ctagacccgc tttgatagct aacggtgtcc gagtcagcaa ttcgatattc ccaaatgaat
    28861 ggacccgcaa tggcaagata tctgccatcg caaatgcagc cccttggatc actgaataaa
    28921 tgacatataa tggcacagca gcaaacataa acatgaactc caatggttct gttacacctg
    28981 ttagaaagac agccaacgct gctgaaaaat acattgattt atatcttgct ttcttatcag
    29041 gatcgacatt acgatacatc gcaaaagcca ttcccatcaa gatacctgaa gaaccaatca
    29101 tttgtcctac tttgaaacga gccggcgtcc agttatgcaa cacaaactca tattgcgaca
    29161 tatcccctgc acctttaaga ttcactaaat cagtggccca agccaaccat aacggatctt
    29221 gcccaaatac ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat ccgccaagct
    29281 gtgtatagtt gataggaatc gtcagcatat gatgcaatcc aaatggcaat agtaaacgtt
    29341 ctaatgttcc aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat tctgcaatcc
    29401 ataaaccaaa attattgatt ccagcctgta cgtttggcca caccacagca agaatcaatg
    29461 ccacgattgt tgaccataag ataacaacaa acggaacaaa gcgctttcca ttgaaaaatg
    29521 acaaagcatc cggcaatttt ctataattat agtacttatt ataagccatt gccccgacaa
    29581 atccagcgat gatccctacg aatactccca tattcaaagc tggtgcttcc aatacacttg
    29641 taaaaaatcc tttaaccatg atcttcgtac cgaacagagt gtgggtaaat gcatcttcat
    29701 tcgcaagcat gtcacttgtg acaccaaaga tagccccagt gatccgattg attaaaataa
    29761 atgagatccc agctgcaaat gctccgcctg ctctttcttt tgcccaactt cctcctattg
    29821 ctaaagcaaa taaaatatga agattaccaa taattgccca accaatattt tcaattactc
    29881 cgcccgttgt gacaagcggc gtccaatcag gattgatcaa gggaattgat ttccctatac
    29941 tgatcatcaa cccagctgcc ggcataactg ctaccacaac cattaaagcc tttccaaatt
    30001 tttgccaaaa ctcaaaacta aatatctttt tcattccgtt tagctccttt acgcaatcgg
    30061 tttcgttaaa cgtattatag cgtgcgtttt cttttttgta aatgctttat tttttatttt
    30121 tctgtctata ccattctata aaacctataa tacgtgtgtt cccttctata attttctcct
    30181 aaaaatttat ttttttaagc ttttttctat gaaatatttt cttttgtcta ttgacttgtg
    30241 caatcggttg cgtttattat gaacgtataa aacagctcat taggaggaga aaaatgaaac
    30301 agatcaaacg actatttgct attgatccat ggaaaatcag cacgacacaa ttagataaaa
    30361 aaaatcttcg actgcaagaa tctttaacaa gtattggaaa cggttatatg gggatgcgcg
    30421 ggaactttga agaaacttac tcaggggacc accatcaagg tacttatttg gcgggtgtct
    30481 ggtatcccga taagacacgt gtcggctggt ggaaaaatgg ttatccagaa tactttggaa
    30541 aagtgatcaa cgccttgaac tttatcgcga tagatatttc gatcaacgat cagctgatcg
    30601 acttggcttc tttggaacca gaagactttt actgggaatt agatatgaaa aatggtgtat
    30661 tatctcgtac attcacaatc actacttcta ctaataaagt acgcatttcc ttcgaacgtt
    30721 ttctgagcat tgtcaaaaaa gaatcagctt atatctattt aactatagaa atgcttgaag
    30781 gtagcggaac agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac gaagacagca
    30841 actatgatga acatttttgg aatgagattg atcgtggaac tatagaagaa atcggctatt
    30901 tgacgactaa aactattcca aacaactttg ggattgaaca atttactgtt actgcagcaa
    30961 tgcgccattt cgttaatgga aaatcagtca tccctgacta tcacgaagat actttagcat
    31021 tacatggttg cttgtctgca gaattatctc aaggtgagac atggtcagtg gaaaaagaaa
    31081 ttatcgttct tactagtaga gatattccag aagcgcagca agcagaaaaa gctgtctcag
    31141 aattaacgaa ccagacaaac aattatatcc aagcaaaaga agagcaaaca gcggcttggg
    31201 cagaacgatg gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca caacaaggta
    31261 ttcgattcaa tttgttccaa ctattctcta cttactacgg cgaagatgaa cgattgaata
    31321 tcggccccaa aggattcaca ggagaaaaat acggcggagc cacttactgg gatacagaag
    31381 cgtatgctgt ccctctttat cttgctttag ccaaacctga agtaactaag aatctgctga
    31441 aatatcggca taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa ggattaaaag
    31501 gcgcgcttta tcctatggtc accttcactg gtgtcgaatg ccataacgag tgggaaatca
    31561 cattcgagga gattcaccga aatggcgcga ttgcctatgc aatctacaat tatacgaatt
    31621 acaccggcga tacggaatat ttgaaaaatg aaggactgga agttttagca gaaatcgcac
    31681 gcttctgggc agaccgtgtc catttttcaa aacgagcaaa taaatacatg atccacggtg
    31741 tgaccggccc aaacgaatat gagaacaata tcaataacaa ttggtataca aatacgatcg
    31801 cagcttgggt cttgcgttac acgcgagaaa attatttaac ttatcaaaaa gaagctgcta
    31861 tcactatcag cgaagaagaa ctagccaaat ggaaagatat cgcagagaac atgtacttcc
    31921 cagttgacga agaattagga atctttgtcc agcatgacac attcttagat aaagatctca
    31981 tgcctgtatc cgatattccc acaagcgatc ttcctctgaa tcagcattgg tcttgggaca
    32041 aaatccttag atcttgcttc atcaagcagg cagatgtact acaggggatt tatttcttca
    32101 atgatacttt tacaaaagaa gaaaagcaaa aaaactttga tttctatgaa ccaatgactg
    32161 tccatgagtc ttccctttcg ccaagtatcc attcgatttt agcagcagaa ttaggaatgg
    32221 aaagcaaagc tgtagaaatg taccagcgaa cagctcgctt ggatttggat aattacaata
    32281 acgacacaga agacggcttg cacatcactt ccatgactgg tagctggttg tcgattgtcc
    32341 aaggatttgc tcaaatgaaa actgcccatg atcagttgag attcgcacca tttctgccaa
    32401 aaacttggaa agcttattct ttccatatca attatcgcgg acgtttattg ttcatctcag
    32461 tcacagatga acaagttact ttcgagttat tagaaggttc tcctctagaa atttacgtct
    32521 atgaacagtc ttattcttta gcggatcgtc tagaaataac gctaagaaag gaggaagcaa
    32581 atgtttaaag gggtattgtt tgatttagac ggtgtcatta ccgatactgc tgaattccat
    32641 tatcgtgctt ggcgaaagct tggtcaggaa atcggcatta cgatcgacca cacattcaac
    32701 gaacagctaa aaggcgtcag ccgagaagac tcactctctt tgcttttagc ttatggcgga
    32761 aaagaacact ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa aaacgattat
    32821 tatttagaga tgatccagac gatcgaacca aaagatgtct tccccggggt cgtgccttta
    32881 ctggattctt taaaagaaga aaaaatcaag attgctcttg cttctgcaag taaaaacggg
    32941 ccattcctac ttgaaaaaat gggtcttaca ccttactttg atgctattgc cgacccggca
    33001 gaagcaacaa acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa agctgtcgga
    33061 ctaaaagcag aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca agccatcctc
    33121 tcaagtggcg ctcaaccaat tggcgtggga cgtgctgaag accttggaga ggagatcccg
    33181 cttgtccctg atacgacttt tcttacgatc gattacttga aagaaaagtg gcatgaccat
    33241 ggataatcca gagatctccg tcgtaccagt tccagagact gaaataaaga aaatcacatt
    33301 gaagaacgac tacttagaag tcgttcttct caattacggt gcccgcctgc accaaatctt
    33361 cgcaccagac aaagaaggaa aatcagaaaa tgttttattg tcttacgatc gtttcgaaga
    33421 tgttctaaca gacaaaagct tttttggagc aactgtcgga cctgtagccg gaaggatccg
    33481 ggatgcttct tggggtgagc atagattgga gaaaaactgc ggatcacacc atatccacgg
    33541 tggcacgaaa ggctggtctt tccaattttg ggaagtagaa gttttcaaaa ctccatcatc
    33601 aataggtgtg gtcttttact taaaagatga attttctacc tatccaggac caatcactgc
    33661 tacgatcact taccgtttaa caaaaaacga attagagatg acaacctctg ctagtagcct
    33721 tgttgaaacc atctgcaatc cgaccaacca tgcctacttc aacctttcag gaaatggaac
    33781 aagagacatc tatgaacatc agttaacggt ctttttagat ggtgtgcttg aattagatca
    33841 agaaaaactg cctactggaa attggacaaa aaaagaaaaa ctgccaatcg actttagaaa
    33901 atccccaaca ctgcaagaga tccttgcatg ttatccagat ggactagatg atgtatttct
    33961 tttgcatcat cctaggcttt ccaaaacttc cttacaatta ttcgagaagc attctggtcg
    34021 ccaaatgact atagcaactt ctaataaaag tatggtatta ttttctacga ctggattcga
    34081 agctgatttc tctgtcaatg gaaaacagat gcattcaaac tacggattag caatcgaacc
    34141 gcaagaaatc cctgatatcg ttcatttccc caaatttgga tcgatcaacc tccatcctgg
    34201 tcaagaacgg atcagtcaga cgatttatcg gttctctgca caataacaag agaaaggtgt
    34261 ttttatcatg gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg ccacctcgac
    34321 cgtctctcgt gtcatcaacg atcatcctag tatttccgag acaacaaaga agaaagtccg
    34381 aaaaattatg gatgaactag gttatgttcc taacatgact gcccgcaatc taggaaaacg
    34441 catttccagt gccatcggcg taatcttacc ccctcttgat tcaaaagagc ggatcggtaa
    34501 cccattttat cttgagatca tggaagcaat caacgaagaa gcgcggaatt atgatatgac
    34561 tacagcaatc gctactgcta aaaattttga attgctgtta gagaacgtcc agcgtatgca
    34621 tttacaaaaa caagtagatg gttttattct agtgtattca gatagtcaag atactgtcat
    34681 tgattattta taccaaaacc acatcccttt cactttgatc ggacagcctt atcatcatga
    34741 gaacgaaatc gtttatgtag ataatgataa ccagcttcta ggtaaacaag caacagaatt
    34801 tttgatccag aatggacata agaatatcct gtttgtgaca aacacgactc atgaaagttt
    34861 gtatttcgaa cgatatttcg gttatcaaaa agcaatgatg atggctggtc ttcctgcgtt
    34921 atccagtgta gtactagagc atccggagga ttatctgtcc tttgagaaaa cgttgaaaga
    34981 aaacgaagcc acagcattgg tggtcattga cgatatcttc gcccttcgca cgatgcagtt
    35041 ggcgcaaatg tatggctatg tcattccaga gacactctcc gtcattagct tcaacaactc
    35101 gatcttctca acattaacac acccttacct tacaagtatc gatatcgata tctccgaatt
    35161 aggacgtgtc gctatgcaaa agctgcgcga attgatccat gaagaattga gcagtggcgt
    35221 acgtttggtc atccctcatc gcttgatcaa aagagagaca gtcgttcctt tagaaaaccc
    35281 atactaaata gctgactcac taaatacgct gaccatttat cctatggtca gctttttatg
    35341 taaaagaaga tactacttct aacacattgt ttatctgtaa aaaagagcgt gggacaaaag
    35401 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
    35461 cttcggaaat aagctgaaat tcacaaaaat ttaaaaagca attttcttga gtttcttctt
    35521 atttcttgga gttaaacgct tctgtcccag cctctttttt ccttccttca attttttaca
    35581 cgtcgacgat tcaataaaaa accgcctaat attgtaaaga caggtgcaag aatcgagaaa
    35641 aaggcgaatg gcaagtaatg caacgcgcta actttcatcg ttcccataat gaatgtgccg
    35701 ctgactcccc acggtacgag agaattgacg gtcgcacctg aatccgctag tgttcgtgtc
    35761 aaataggttt ttgataagcc aacgtcatca aaagaacgct tgaatgtttc tcctggaaga
    35821 atgatggaca gatactgttc tccaacgatc agattgattc cgatacagct caaggcagta
    35881 aacccgacta aacgagaagg tcggtccatc ttttctttta attcttgaac gatggtatca
    35941 acgatcagat atttgatcag taacccacct aatgccaatg caagtaaaat caaactagca
    36001 gaacctaaca tactcatgat cccgcctcga gaaagcagtt catctagctt tggatcaggt
    36061 gtatttgcga cataaccatt catcaagatg tctgctgctt ttccgaccgt caaacttttc
    36121 tcatggataa aagaaagaac gagagcagct gtcgatcccg ctagtaatgt agggatagcc
    36181 ggaatttttt tgattgcccc cccaaataaa ataactagtg gaatcaatgt ccaaggagag
    36241 atccaaaagc tcgctttcat gttttggatc atttcattga tactagctag atcggctgaa
    36301 tgactgttat gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc tgggaaatcc
    36361 gtataacgca taccagcgat atgttcgaat agattaactc ctccaatacc cgctgccaag
    36421 ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa aggcaccgga aacgactgcg
    36481 cctgtagtca ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa agcaattcca
    36541 atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc cgacaattgc acaaacaaga
    36601 aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa atccataaac cataatcgta
    36661 gggatggttc cagataaaat ccatgaagca actaagacac caatcaaaag aaaaatcagg
    36721 attggaatga tccctggagt gattcctttg atgatcccgt catggatctc atcccatgtg
    36781 aactttttaa atctgccata aaacaacaaa cacatgaaag ctgcaaggat cgggatatgt
    36841 ggcgagagct ggaatccgat gatcagtact cctaggagtg ccaacaacac gatcaataca
    36901 gcgatacttt cttttaaaga aatattttta ttcatcgtcg atctcctatt tcaaatgaaa
    36961 aggccctgcc atgaaaaaac tattcacttg gcaggacctc ctactaaatc agcgacaaaa
    37021 cgtcggctga aagacttatt tttttatttt tcttctaaac ttttttctac ctcaatcccc
    37081 caagaatctt tcttgaagtg attgatgatc aatggaataa cgaacatgac cactgtgata
    37141 gctgctacta ataatgcatc actcaatgtc gctgtaccta atgtagcaaa tacgacaacg
    37201 atgatagaga agatcagcat gcagctgact acccaagcta atccgtttcc gtttttacca
    37261 attcggtaag gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa tgaaacagca
    37321 atcaaaatgt acacgataca ataaataaca gttgtagcat ttgtaagtgt caagaataca
    37381 gcacttacgt catccattac accataaagc aaagcaaata atgaaataac gattgattgt
    37441 gttagaacga catttctaga tacgccgtat ttgttttctt tatggaatcc gaattttggt
    37501 ggaaggaatc cttcacgagc tacttggatg attgtttttg atggtccagt aacccaagct
    37561 gataattgta aaagcacacc aataaatacc atgaagctga agatatttcc gatcacttct
    37621 ggtaagccta ggatttgaca gtcaatcaag ataggctgcg tgatatttgc taattccatt
    37681 tttccatctg gtacgacatt tgctacaagc atcgcattga tgacattcaa taaaactaat
    37741 ccgatcaaag cgataaatac gccttttgta taattttttg ttgcgtcttt caaacgaggc
    37801 atgtaaacag aactcatttc gatcccaaca aaaataaatg tgataccagc taagtatttc
    37861 aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc acctagatag
    37921 ccgtttgaga ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa catgatgact
    37981 actgggatat atacccctag ccatacaccg atgttcccgc cgacttttgc catatcgaat
    38041 ttcaagttca agatcgtgat gatccagtag ataactaaaa tacaacctaa aacgaaccag
    38101 tgattattgc caagttctac attaccaaat gtgtttccta acaatggtcc taaagtagaa
    38161 gcgaccatta ccatacctgg gaacatttgc acccataata gccatgcaac gacaaatccc
    38221 catttgctgc ccagtgctgt ttttacccat aactgaggac cgccttcttg aggaagcatc
    38281 gtggaaagct caccggaaac cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt
    38341 gaataaaaaa tcaatgtcca accgactgcg gctaacgtag gaatactccg aaccgttgca
    38401 cacagtgcca ttgtcagacc gataaaggtc ccaagaccca ttttacttac ttcagattta
    38461 ctcaccgttg acactctcct tgattttttg gataaaataa cggtgtcttt gaagtagttt
    38521 cagcaataaa cgaaacaatt cagcgaatat ttgaggggat tctttgagtt tattgcttga
    38581 agggtttctt caaagacaac ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc
    38641 tcttcagatc caaacatgcg tcagaaacag ttagtattca agctgttttc taacatgatt
    38701 accttgctta ttttgcttcg cttgccaata attggtctgc gtagattttt tccaattttt
    38761 cttgaagagc tgctttgatt ttttcagcat attcttcaaa gtgttcttct ttgttcatgt
    38821 atggtgtcat tacgctggca cgcaatacag ttacttttcc ggcacggttc cattcttcat
    38881 ctgagaatcc tagttggttc acaaattgta atgggctgtt gccatagtct gggatagcaa
    38941 agtcagtatg tgaagttaag aactcattac cgtaaatgct tcctttcacg tatgaagaat
    39001 aatcatatac gtcatggttc aatttattca ttgcaactaa gtcatcgttg cctttttctt
    39061 tgaatacata gtctaccata ttgaaatctg gataagttaa tggatgaact tcgatttctt
    39121 tgtctcctac tttaaatgac aagtcattca agaagttgta gaaacgatgt gcaccttcga
    39181 ttgatgcacc cattaattta ccatatcctg tgacattcaa aggtaagaca tggtgtgctg
    39241 cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc caagatataa gccccaagta
    39301 atgctgggat atctgcgcct ttttcaaata catatgtcgc aaagtaagag ataacgtctc
    39361 tcatgcggat atctttgatg acgataccac cggcagaata tggaacatag cccattttat
    39421 gtgggtcaat cgttacagat tctgcttctt cgattgcttt atatgcactg tgtacttctt
    39481 ctaagatata atctttgttt tctgtaaaga cattgtattt gtaatgaaca tcttttaaat
    39541 cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat
    39601 aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg
    39661 caacgatttt atcgataccg tcgattgctc cttcttctgt tgatcctaca acaccgacta
    39721 cacctaggat tggtgttttt tcagctgcta gaccacgaac gattttttca agttcgttga
    39781 tgtccatgcg atagttgtgg tcaactggca ctggaatcac ttggtctaaa ccgataccga
    39841 tgatgtcggc agcttttagc catgaatagt gtttagtttg tggaaccaac cattttccta
    39901 atttttctaa atgttttccg ctacgtgctg agtgagcttt gatttcgtcg attttttctg
    39961 ggacgctgtc taacagattc atgatttctt ctgttgacaa gttcattaat tcccaatcgc
    40021 tttttccagc aactagttca ggtgtcactt ctttcattgc aagcggcaat gatttgatgt
    40081 tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg atgtggcccc
    40141 agccatcttt atagctcatt aattttgcaa attccattcc tacttcttct tccatttggc
    40201 ttgttgcagg agaagattca taggcaacgt tattaccatt ccatagcata gcaaaattgt
    40261 acgctagtaa agatggcatc aatgtttcag agttcatatg accccagtaa cgtccggcat
    40321 tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat ttctgataga acatcctttg
    40381 ttttattcac agtgtgttca aaactagcac tgcttttttc ttctggcgta ataataggca
    40441 tgtcttgagg catgtagttt tgacgccagc ctaaatgttc atccaccaat tcattcaaca
    40501 aagctttata gatttggccg ttctcagctt tgtccccaat gaatagggca tttaggttta
    40561 gatctttcga caatgattca ctcatagtga aaccctccta aaaaaatatt taaattttgt
    40621 cgagccctac attacacctg ttcttctatt ctgtaaacta agttttggcc tgatttttaa
    40681 tcatatttta atatgtatca attatccgaa ttgttcacac ttttgaaata attaaaaaaa
    40741 acgagggaat ttacaaatgt aaactccctc gcaaagattg tattaacgac aaccagcgtt
    40801 ctattttagg atgggtttca aaattggaat gttgtaatag tatttccctt tttcaaggta
    40861 aaaacttact aaattctaat caattacttt ggccaaaaag taatttttct taccttttcg
    40921 aataaccaca aacttaccat cgaattcatc ggatggattt atgacaaaat ttaaatcggt
    40981 aacgcggtca ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt
    41041 agatggttca atttttgtgg aaacaagcag ttccacgata ttttcaggtg tgcttgaaat
    41101 ttcaacgttc ggcattttac cgaatccttg cgcgatttct tccgcattta gttctttaat
    41161 atttccagag aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt cttcactatg
    41221 cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct caggttctgt
    41281 ctctactttt ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa agaatttcaa
    41341 gtatttcact acatcgcgat catcttgatt caaccagaat tggtagaact caaatggtga
    41401 agttttcttc ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc
    41461 agccttcagc atcaatggga tcgttaaacc gaacgcttct gcttctggtc cttctaattt
    41521 tcggattagg tctaatccag ctgtgatatt tccccattgg tctgctccac cgatttgcaa
    41581 ttgcacatcg tatgttttat gcagatgcaa aaagtcgata gattgcagga tttggtaagt
    41641 aaactctgta aatgagattc ctacttccaa acggctggca acgatatctt ttgctaacat
    41701 cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag agatttttga
    41761 caaccagtcg tagttgttca caaatgtgat atttgcatct ttaccaaaca attttctcat
    41821 ttgattggaa agtgcgtcca cgttatgttg cacttgttcc atcgtttgca acacacgttc
    41881 tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca gaatatatgg
    41941 atgatggcct gccaattgga atcttttcat catcataaat ggaatcaaat gtccgatatg
    42001 catcgaatca ccagttggat caacgccgca ataaagtgaa atgctctttt cttcaacgag
    42061 ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag ttaattcatc
    42121 aataatattc attttactga cctccgtgta gtttttttat gaaagcagga acctgctagc
    42181 atatgtaatt aattatttct tcaggatagg ataggaaata aaaaaagtcc cttggcatat
    42241 aagccaaggg acgaaatctc gcggtaccac ccaatttgta tgtctagata tccaacatac
    42301 ctctcgaaca tttaacgctg tcgcgtggca atcactgcca aagactcctg aaatgtaatt
    42361 cgctttaaag catgtactgg tttgcagcga ccaccagctt tctgaaacat tggctttaac
    42421 aactacttcg tttcgatctt cgtcttgtta ttattaatat gtgtctatca tagagcgcaa
    42481 ctttttcttt gtcaacagaa aaaggatatt ttttttaaat tcctcatttt ttcttttttg
    42541 aatccgctct aattccgaac attttatttt caattattgt taaaagaaat aactttttct
    42601 gtattttttc tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt
    42661 acaataagga atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa
    42721 aagcaaaaaa ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt
    42781 ttgttttttg tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata
    42841 acagaaaaag caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat
    42901 ctggctcaaa gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac
    42961 ggatctactg aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc
    43021 gactggtctg atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg
    43081 caaaaagcct tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa
    43141 gaattcaacc gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat
    43201 gatttgatcc aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa
    43261 ggaaaaaaac cattggttct ttcacaagat gatgtcagct attatgaata tatggataat
    43321 tccggttttc ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa
    43381 gagaaagata aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc
    43441 aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac
    43501 aacggagttt taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa
    43561 aaagagatta aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt
    43621 gcttcccata catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa
    43681 gacaatgaat tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt
    43741 tatccatttg gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt
    43801 gcttacttga agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct
    43861 tggggacaat taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt
    43921 tttcaatctg atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat
    43981 gtctatgaca aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag
    44041 ccgaactgac attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg
    44101 attcccttga tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc
    44161 tccggcttag aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa
    44221 tatttcaaat ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg
    44281 aaactttctt gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta
    44341 tagtatgtca tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg
    44401 ttttcactca tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc
    44461 gcttcttgcc ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg
    44521 ctgccaaaat atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc
    44581 attgtcactc cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc
    44641 tctttctttt tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat
    44701 tttaaacttt tataatatat atagttcaaa tttattctga ggtttattat aactatctat
    44761 tgagaaagta tcaagctagc aattccttgt ttatgaattc attgacagaa atttataaaa
    44821 agaatattat gtactaaaat aataaaaaaa gttttataaa aaggtataaa acctttttat
    44881 taaactattt aatcttgttt agtatatatt tttagaaaaa ggagtggggt ttttatggca
    44941 gaaatcgtca aagtcacaca tttgaaaaag agctttggtc gtttcgaagc tttaaaagat
    45001 gtctctttca ccgtacattc aggtgaagtc gttggattta tcggaccaaa tggttctgga
    45061 aaatccacga cgattcggac acttttaggg attataaaaa aagacggcgg aacggcaacg
    45121 atttttgggc aagatgtatg ggaagatagt ttatctatcc atgaacgtct ttcttatgtt
    45181 ccaggcgata tctctttgtg gggaaatttg actggaggag cgatcatcga tctcttcatg
    45241 aagcttcatg gaaaagggag tgctcaaaaa aaagatgaac tgattcaacg tttctctctt
    45301 gatcctaaga aaaaagcaaa aaactattca aaaggaaatc gccaaaaagt gggcttgatt
    45361 gctgctttgg ctgttgattc ggatctctat ttatttgatg agcctacttc tggtttagat
    45421 cctttgatgg aaacgatttt ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc
    45481 atcctacttt cttctcatat tctcagtgaa gtcgagcgac tagcggataa agtcgtgatc
    45541 atacgcgaag gaacactcga tgaagaagga acactcgatg aattgcgtca tttgactcgt
    45601 tcgaccatca ctatagaaac agaagcagag aatagtcact tagcacaatt gaacggtgtc
    45661 catgactttg tgcaaaaagg tcacctcgct caatttgctg ctgataacga agctttcgat
    45721 gtgattttag ctgaagcgtc caaattaggt gtgaaaaaat ttgaatctat tccaccgact
    45781 cttgaggatt tattcatgag acattatgaa ggatgaggag gaagcgaaat gactgataaa
    45841 ttttcacgct ggtccaccct tttttctcaa aaccttagcc gtgactggaa aaagatcctg
    45901 atatggctag tcggtgtcac tgctttttct ggtggttttg tccctgcgtt tgaagaattg
    45961 acttccggac aaggattagc tggattatac gaaaccttgc aaaacccggc aatgattgca
    46021 atggtcggac caactccagc ttcgaatgct gctgagtata ctttaggagc gatgtatgca
    46081 aatgaaatgt tgcttttttg cagtttgttt gctgctgtga tctctgtttt acacgtggtg
    46141 agccaaacga gaaaaaaaga agaacttggt ctgattgaac ttattcgttc ctttcgagtc
    46201 ggacgtcaag caaactcttt agctgtcatc gctgaaagta ttctgatcaa tttgctttta
    46261 gcaatatcga tcagctgtac gatgattttt ttccaagcgg attcgatcac tgcagaaggt
    46321 gcttggcttt ttgggctatc tgtaggtagt atggggattt tggggagtgc cgttgcactg
    46381 tttgctgcac aactgatgcc aagttcagcc agttcgaatg gtctgtcttt agcgttttta
    46441 ggtggacttt accttctacg agcaataaca gatgtagctg attcctccct ttcgatgtgg
    46501 aatcctttag gctggtctta tttgacctat ccatttacaa ctaacaactg gattcctttg
    46561 ctttatttgt ttttgttcag tctaatcttg ataatcagtt catttgtttt agaggaacat
    46621 cgagatttga acaccggcta tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct
    46681 cttttatcct tgcgtggttt attattctat ctaaataaag ggatgacaat cagttggctt
    46741 atcacttttg gtttgatggg ggcggcatac ggctctatct acgggaatat gcaacagttt
    46801 ttagaaagta acgacttaat gaaacagatg tttatccaat ctggcgtctc aattgaaaaa
    46861 aactttacaa gcacgatcat ggtagtcttg atttgtctag ctgctatttt accaattgct
    46921 agcatcaatc gtttatttac agaagaaacc cacggacgta tgaatcagct gttcgcaaca
    46981 aaaatttcta ggaaccaact ttattggacc aatatgttac tagctattct atttgctata
    47041 ttggcaatcc tgattagttc tggtgcacta gggctttctg ctttggcttc gatgaaggat
    47101 gcgaaagaca tggatctatt gatgtttctt aaagccgggt tcaatttcct gcctgccgtt
    47161 ttagtgttta tcggaattag tggctttcta ttcggatggc ttcctcgaat cggtaaactt
    47221 gcttatatct acttagctta ctcctttgca ctcaattact ttggcggaat tttggattta
    47281 ccagaatggt tttccaaaac agctgtccct agctgggttc cacggatgcc aaacgattct
    47341 tttgatcttt ccgtgttcct tacgttatca tgtatcagcc ttgcactgtt cgtccttggt
    47401 tctattggtt ataggcgaag agacttacta gaaggataat aaaaaagcat gtacaatttc
    47461 atgctagcct ccaaatctgg gtcaaaaatc caacatttgg aggtcatttt tttatttaca
    47521 agtcttctaa ctgctcttta tcaatgatcc agacttctcg attctttctt tttacataac
    47581 cattctcctc taataattta aattttctag ataaagtttc gggggtcgta ccgataaatg
    47641 cagcaagttc tttcattttc attggcagga aaaagtgatc cttccctgtc gctttagcta
    47701 agtcaagcag atagacagcg actcgttctt ctactttttc catcatcaaa aaagttgtct
    47761 gctgctgtac ttttgttaat ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg
    47821 gataatgcag taagagttcc gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt
    47881 ctagtgcttc gccaaataaa gactcatttg gaacgcctag caactggctc tctccctcat
    47941 atccgcccgg ctctattact cgtaaaagct gttcctttcc gctagcagat aattgataca
    48001 ctttcatact gccggaggcc acgatcacta attgaggttc tccattcggc gcaagaatct
    48061 gttctccttt ttccacatag tgatgtttga caagtgcgtg tactttttct tgatcttgct
    48121 gtactaagtg attgaacaaa ggaacaaatg aaacacataa atgatcatga ctcataatct
    48181 ctcctccttc taaacagcta tttctaataa agaaacataa cttcccttat tccaactgat
    48241 aattttcttt tatcatagca taaacgtcaa gcgaaccaga taccagcaaa tagagtggat
    48301 ttctttttgt cccttgcttt attttccttc ttttgagacc ttaagtaatt gattcctatc
    48361 aattttttaa atatttactt tttgaataat caaaaacaaa ccttcactcg caagccgaga
    48421 gtaaggtttg tttttcgcat tgcttatttc gtgttgtcta ttgaaatttt tccgcaccta
    48481 gcttttgata tagtggtgct agttcttgta aggtttcagc ggttatttgc tcaacgtgtt
    48541 cgatttcttg cggatagaag aatagtttac ccatttgaaa ggttcggtct ctcatcgctt
    48601 ttttcaactc ttcttgtttc tttgtatcgg acaaatagtc agacaaagct aagtggtcgt
    48661 caagttcatg ttctggttgc ggccatatgt aataatcctt gatatcgact gttttgctcc
    48721 actctttcag tatcgcaagc aactggttat acgctgcaat gctaccatat cgttcttcgc
    48781 ttttataatg ttggtacatc aaatagattt ggtattgttt ttgattcaac aaaactgcta
    48841 gacaggcatt ctcgtcttga cgagattcgc ttcggtacgc gctccaaaaa tgactgcgta
    48901 aattccagcc attcgtccag ctttcgattt taggtttgct catgccgtac atatcaggca
    48961 aaagtgcagc tgtctggagc tgaatctctt tccatttttg ccagtgttct ttatattctt
    49021 tcttgatttg ttcaatctta tctgttggat ggctctcttt gagctgtttg aatgcgtaga
    49081 ttggtcgatc aaacaactga taatctgatt ttccaagttc cattgtttcg cctctttccg
    49141 ttatcttttg tattctacct gaattttgcg atatggacaa atagaggttg tgaaaaagct
    49201 gttctgcatc aagtcataag aacagccaaa caaaaaactg cgatgagcat caccgcagtt
    49261 ttattgaact taaggttgat tgatcacatc taaaagcgtt gaaaataaaa tggcactaga
    49321 agcagctcct gggtcgatat gtccgattgc acgctctcct agataggaag ctcgtccttt
    49381 tttagctacc aagactttgg tgtgttcttt tgcctggtca atgatttctt ttgttaaatt
    49441 ccctgccttc aatgcctctg tgactggcag ccaaacatct aacatcgttt tatcgtcagc
    49501 ttctgccttt cctcggcttt gaatacccgc tgttccctgc tcgatcactt cgcctaattt
    49561 ttcttgagaa tcaatagaat cgacctcttt tgtcgctttc atcatgttca tgaatgcaga
    49621 tccgtataac ggaccagaag ccccaccgac tttcgatatc atcgccatag agaatacttt
    49681 gaatgtatcg ctgattgttt caggatgatc ggcttgaaaa gcttcttcat aagctgaaac
    49741 ccctcttccc atgttatttc catggtctcc atcgccaatc ggggtatcta gatcactgag
    49801 atattgtttg ttctgattga ttgcctctgc aaattgcgtg agccattctt ggatttcttg
    49861 aacggttagt ttcatattat caccctttta tcttgagatt ttttaccatg aaatcgtctg
    49921 tacattgctt tctaaagaat ctttccattg tgtctccgtc aagtcgatca gtgttagaga
    49981 aagaccttgc atatctagtg aggtcataaa attaccgact tttcggaaaa ctacattgac
    50041 gcctctttct tccaacagag ctaaaacatc attcatgaac acgaactgtt ccatcaaagg
    50101 cgtcccgccc attccgttga ctagtacacc tgcttctttt ggtaaattcg agtaatcatc
    50161 taaaatctta gtaacaagtt cctttgccag tactttagaa ggctgcatct tttctcgacg
    50221 atagcctggc tcaccgtgga taccaacacc atactcgatc tcatcttctc ctagttcgaa
    50281 tcctggtttc cctacttctg gtacagttgc tgctcgaagc gctagaccga tcgtcttagt
    50341 tgcctgtacg accttttctc ccagttcttt caactcagct agggaagcac cgtttcttgc
    50401 tgcatcgccc acgattttgt gtacaaggac agtacctgca actccgcgtt ttccagcagt
    50461 atacgtacta ttttcaacgg cgatatcatc atctacgatc acactttcta cttcaatatc
    50521 gtccatagca gctaattcct ttgccatatc gaagttcaat gcatcccctg tataattctt
    50581 aacgatcagc aaaactcctt ttccactatc ggcttcctta atagccgtct gaatctgatc
    50641 tggagttgga gaagtaaaca catcaccaag caccgctgca cttaacatcc cttcccctac
    50701 aaacccggca tgggatggtt catgtccgct tcctccacca gaaaccaaac caacttgttc
    50761 cgctttctga ttcttggcta caacacggga agattctaca cgatgaacaa gttctggata
    50821 gctttttact aatccttcca gcatttcttc gacaatattg cttggatcat tcatgatttt
    50881 tttcaattta ttcccatcct ttctttccgg cattttgtgc ttctgttaag atttgctgag
    50941 tatcatctgt tataccggca gtgatggctg ctgcaaaagc tccttccact aaaggcgcat
    51001 ccaccagatg ataacgttgc tgctgctctt cttccaacat gtcttttgcc agttccgagt
    51061 tcaataccgc gctaccgata tccgcaaaaa caaaataact agctgcatcg cttgattcat
    51121 tgactgcttc tacgattttc atcgggtctg atccgatctc gccatttatt ccgccgagag
    51181 aaaaaatttg cacctcttcg gatttttgca tctgctcaat catttcttta ataccatctg
    51241 ttactttttg actatgagaa ataagcaaaa tacttttttt cacaagaatc ttcctttctt
    51301 ttacatagtt gctaacgatt ggtttcgctt tcattaatga acaattcaag tatataataa
    51361 atttctaatt ttgggaaaat atgtgtagtg cttcttgaag tttgttatac tttaattaaa
    51421 agagaggagt gatcgaatga ttacaacaac aacgaacagt gtagagggaa aagccgtagt
    51481 agcttataaa gggatcgttt ttggtgaggt cattactggg atccatgcat ttaaagactt
    51541 ggaggcgggg ttgcgaaata tctttggcgg ccgctcaaaa agctatgaaa acgaactaat
    51601 gggtgcaaga gaagaagcac ttgccgaaat ggccgaacgt gcaaaagcat taggagctga
    51661 tgcgatcatt ggcgtaaaaa tggactatga agtattaggc agcgacaatg gcatgctgat
    51721 ggtcacatgt agtggaacag ctgtctccac gagataagta ggggacatct gttttaataa
    51781 ctaaagagag atgggacatg tagtggcact caagaattag atttttcagt ctaacttcga
    51841 ggtactctac cttcccatcc tctctttttt atttcttttt gcggatgatt ccgtaagctg
    51901 ctgcgactaa cacacccgcc cctgctaagg aataactgac agaagctgtt ttttcatttg
    51961 tttttggcaa agtctgactt gttgatttgg atggctctgt cgtttgctga actgtttctg
    52021 tcgtgcttcc agaggtcact gatttttctc ctgccgaatg caaaatatct tttattcgtt
    52081 ggaaactagc tgctaagtca gctttttttg cttcaaaatc attagcaact gtttgtgctt
    52141 cgctgatctt tccgtggatt gcttgactgg ccgctgtgat atccgcttga gtttgactta
    52201 gctttccaga agctgtttga gcaacctgat ttaattgctg attgaattct tttgcttgtt
    52261 ggatcttccc gtcaagatct gtttggacag cttccgcttt tgctttcgct tcggcaagct
    52321 ttttctcaag ttgtgcacgg tcatttccca ctttattttt aagatcagct aaatcttgat
    52381 cgattgctgc ttggtcttct ttcaatttgt tttttgcagc ttctaatgct tcttcgagtt
    52441 cttttctttt attttccaag tcttgcaaat gatcgatttg gtcagaagag gtagaactat
    52501 ttattgatgt atcttctgaa gatgaaatcg tttctcctga attatctgtc ggtacattgg
    52561 aagtcatttc tgtttccgag gcaccgtttt cagcaccaga agagcttatg gttgttgaag
    52621 gttctgtttg agcaggttca gaagtttccg caaaagctgt ctgtcccaat aagaaaatac
    52681 tggaaacagc aattggccag attaacggct tcattctttt tttcatcact tttcccatcc
    52741 ttactacttt ttaactaatt tcttattata agcaaaaatg tttggaagaa ccataaacac
    52801 aacaggctgt ttgtctaaaa aacggtgcat cttttatttt tttgtacgtt ccgggctttt
    52861 ccatgtcccg cttccataac ctaaaatcgt tttgactgct ggaatgaaag tcgtcacgat
    52921 cgttatcgcg ttcactatcc agaacaaaag catatatagt ggtgcgaaaa gaaggtattt
    52981 caattttctt cgtcgatcat ctacaatcag cgaagctagc aactggaaaa atcctgcgac
    53041 catttcaaag cagatgaaca caaaactgaa tgtaaaagta atataaatct gttcgtagtt
    53101 tccttgatat acataatgcc cgattaatcc agcaaaaaga actacggaaa tacaaaagaa
    53161 tattgaccag acaatactca atgtttgatc aatgaagatg atcgttcttc caatatgttt
    53221 aaaaggatgg agcatcactt ttttgaaatt cgtcagccag acttctgttc caccttttgc
    53281 ccaacgtttt ctgtgtcgat atagcatctt caacgtcaca ggtacttcca tgaaaaagat
    53341 aatctcagga gcaaaaacag ataaccagtc atttaactga tgatcccaag caatactgat
    53401 gtcttctgtc gctcgatctt gtctgaatcc gcccacatcg atcaaggctt cttttcgata
    53461 catcgtattt gctccactgt acgcataaag accaccgaaa acagctgact gtgttcgttt
    53521 aatgatgccc acgatacttg agaattcaac cgtttgagat tttgcaatca atttggtacg
    53581 attttggaca tccatatttg cagtgaccgc agcaatatgt cttgcaccag ggcgaatgaa
    53641 ataattcaca tacctaatca gtgcatctgg ctccggtacc gtgtctgcat cattgcttaa
    53701 aatcaatttc ccttttgcaa aggcaagtcc gatattaaac gcgtgggctt ttcctttatt
    53761 tttttcgatc cgaaccacac gaagattagc gtattttttc atcagtctag ctaatatttc
    53821 tggtgtttgg tccgttgaac catcgtctgt cactaatacc tcatagttgt gataattcaa
    53881 tttcgtcatc aaatactcga ttgtatattc gatcacaatt tcttcattat gggctggcac
    53941 catgatcgtg atgaacggtt cgacactcaa gggaacatct acccattctt tttgcttatg
    54001 tttgtataaa aacacataac accagacacc aataaaccat acaaatccgc ctaaaatcgg
    54061 ataagaaacg agaaaaaaat acataataga caaacaagta tctaagaata aattacctgt
    54121 atgggtgatt gagatcattt cagttccacc ccattttctt tatacaaatc tgcaatgaat
    54181 gtcgtatcta gatttttctc ttcggagaca gaataaaaac ggaccgtttc tctctcttct
    54241 tttgttccaa aacgttccgt atacacttct tcaagtaatt cttgtctttt tttcaatttt
    54301 tcatcttcat attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta
    54361 gtcaatacaa atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag
    54421 aaatacagca ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct
    54481 ggtctcgtta atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa
    54541 agagccacta acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct
    54601 ttcaccaaaa ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt
    54661 tttttgttac gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct
    54721 aaatttttcc taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt
    54781 ctttttatca gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg
    54841 acaatgcttt tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca
    54901 tctccatcgt tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga
    54961 ctcaaaggaa tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt
    55021 agcgtttggg tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa
    55081 accaaaatct cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg
    55141 ggttcttttc cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag
    55201 gttttgaaaa gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga
    55261 tggatcggca gatggtagtc gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa
    55321 ttaaagattg cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg
    55381 atcaatagac ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga
    55441 aatgctggta catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct
    55501 tcatcaacag tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt
    55561 ctttcaaaca actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt
    55621 cgatattttt tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca
    55681 gaaatcaatt taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc
    55741 tgcacagcag gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc
    55801 tttttcgttg cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt
    55861 ttttcgatcg tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa
    55921 ttcggatgat ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta
    55981 cgcaaacgtt ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct
    56041 tcaccttcta tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt
    56101 ggatttcttt cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta
    56161 ggtgatagaa tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac
    56221 gcaatacgct gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata
    56281 ttttgacttt tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata
    56341 gccttcattt cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata
    56401 gctacagacg tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt
    56461 tctttaataa ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg
    56521 ttattgtaaa aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca
    56581 tcatctgtga aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag
    56641 tctacaacga cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga
    56701 ttttctgcgt ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc
    56761 atcgaaaaag tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc
    56821 atttctttca acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg
    56881 ttttttttat caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt
    56941 tgtgtgttga ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc
    57001 tcttttaaga gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc
    57061 ttcaggtcgg acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc
    57121 agactgcttc aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat
    57181 atattcttgt tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg
    57241 tttggcttcc ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat
    57301 ttttcctttt gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc
    57361 tgcaatgttc attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg
    57421 cggtgcatac gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc
    57481 tgttgctaaa atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata
    57541 aatctcgcca gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata
    57601 atagcttgca tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt
    57661 ggctgttctt tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc
    57721 tgttccaata ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct
    57781 accttgacga ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg
    57841 ttttacaacg atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc
    57901 gtctactagg atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt
    57961 atcaggacga acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc
    58021 cagtcgcaat cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc
    58081 tactccttga tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg
    58141 aagaatatgt ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc
    58201 tgccatttcc aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc
    58261 taatgcatgc atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc
    58321 ttctgttatc cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc
    58381 atcgtactgt tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg
    58441 aaagatggct gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga
    58501 ttctggcgtt tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg
    58561 caagccgcaa tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc
    58621 ttccattaat cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat
    58681 aacgattttc atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac
    58741 gtacattcac tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca
    58801 tccctgattg acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac
    58861 tttgtaatgg aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc
    58921 taacatcaag taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg
    58981 attttattga ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac
    59041 ctcatggggt atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt
    59101 ttttcactac tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa
    59161 atagaggaga atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta
    59221 cagagggaca aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg
    59281 tcatcactca attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg
    59341 cagataatct gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta
    59401 aactagaaca agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc
    59461 gttttccata ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg
    59521 ataaaagctt ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt
    59581 tatctagact ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc
    59641 gccaatgcta tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt
    59701 gtaccagcag gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag
    59761 ccttagtcag tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt
    59821 cgaagaagct acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca
    59881 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt
    59941 accgcagatg caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga
    60001 cctaagtatt agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct
    60061 aataccgaag gtcagctgat tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta
    60121 gtttcgagta ttaagaaaaa ttttgtgaaa atggatttct acatttttgc ataaatttgg
    60181 cttaataccg agaaactggc ttttgaatac cgtttaagga agaggttgta aaatcagcgg
    60241 tttgacctga gtattagaga aacaaacgga aaaatagctg ctcatatttt gccgtttttt
    60301 gagctaatac cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca
    60361 tttaactccg aatcacaaga aataatttca aaaacaactt ctcctgtttt tggttcggtt
    60421 ggcttattat cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata
    60481 ggtcacaccc cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac
    60541 aaaagattgt aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg
    60601 taattctggg aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt
    60661 gccataccac ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac
    60721 aaacataaag ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata
    60781 ccatgccata gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac
    60841 cttccctaag tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga
    60901 caaacctgag acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata
    60961 aagataaatc ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat
    61021 ttctttacgc cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt
    61081 ctttttattt tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt
    61141 tacagacact gatattatga gctaatttga gataatcact tgctgttttc atcgtttact
    61201 cttctttctt cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat
    61261 cctcgctcta ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa
    61321 tcgtaacctg tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc
    61381 acgtggatta tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa
    61441 actaacgctt ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctga
    61501 cgttcttttt tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc
    61561 tgtgtccgat aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac
    61621 atatccattt tatgagcaat ccacaaagct tggctttctc ctgtttcttc caacccgcaa
    61681 ctgatattta ggacgaagtg cttcccgatc gaaagccata gcagccggca caaaatcttc
    61741 atgatctccg gcgaatttct tgatttctcc ataatgggtc gtggcaatga tcaatgcccc
    61801 ttgctcatac atagattcca tgattgcgat cgctagtgca gccccttcat ttggctctgt
    61861 tccactgcca atctcatcta aaagtaccaa cgtattcctt tttatctttt tcaaaatagc
    61921 agcgatgttt tgcatatgac cagaaaatgt actcaacgca ttttctaaat tttggtggtc
    61981 accgacatcg acgaaaatct catcaaagac agcaagctct gtcccttctt ttgccggaac
    62041 ttgcataccg aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt
    62101 gcctccggca tttgctccgg tgataatcaa gcctcgatag tctttaccga tttcaacatc
    62161 caatggtacc gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc
    62221 cgatttattg attctaggtg tgcttccatt gatttctcgg ctaaattttg cccgagcaaa
    62281 gatgatatct aacaccgtga tcgtttcgat caataaatcg atcacttctt cgttttctgc
    62341 gatagcacca gtcaatgccg ccaggacttg atattcttca ctgatttctt cagcttttaa
    62401 caactgatag tgttcattca atttagaaat cacagttggt tcaataaaaa ccgtcgttct
    62461 tttatttgat tcatcgataa tcgttccatc tattttattt ttatagctgg ctttgatagg
    62521 aatcgtatag tattcgcctt tttgaacaat cattgcttct tggatcattt ctttgttttt
    62581 cggatgacgc aagaatttca gtaattttga ctggatttct ttttcgatta gttgtaattg
    62641 tttgcgtact ttacgcaaat tacgtgacgc gtcgtcactg actttattat tttttatttt
    62701 ctggtcaatc aactcttcta catttatcaa atcaggaaga tgtttgctat aagcaaacaa
    62761 caagggtgtc tggtattgat tcttatcaaa aaactttgtg atcatccgac tgcttctaag
    62821 aaaatccgca tattcgatca aatcggttgg ctgcagaatc aacccttttt tgacttgttc
    62881 tgtcagtgca tcgatcctcg gcaaccccat aaatggaaca tgctgactgc tttctaagat
    62941 caagcgtgct tctcttgttt cttcttgacg atccaagaca gtttgtaggt ttgtcgagac
    63001 cgtcatatcg ctgattcgtt ttttgctata gtttccaatt gctcttgctt ggatttcctt
    63061 tttgatccga tcaaattgta attgactaat catttcgttg tccatattct ctccttttct
    63121 tgaagagata cctgtcccaa aataaataag ggcctagaga taaataaaag gatagaacac
    63181 agcttaaaaa agtcatgtcc tatccttatt agaatacaaa gatattgttt tcctgacagg
    63241 taccgtttca taagcacacc aaataaactt tgtttattgg ctattcgact tactgattag
    63301 ataccagcta cactcacaaa acatacccca ttcttctagg tcttatttat tttccactta
    63361 actatagcaa gacacatttt ggttgtcaaa ctttagtatg ttttatagat ttacaccatt
    63421 aatgaataga cacctaccgc gataattgct ccgatcattg gacctgcgat tggtacccat
    63481 gaatatgccc aatctgaagt tcctttatta gctattggca ataccgcatg tgctaatcga
    63541 ggaccaaagt cccgagcagg gttgattgca tatcctgttg gccctcctaa tgaaagaccg
    63601 atggctaaga tcaatccacc tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt
    63661 ccaaaagcta gcaacccaag aactaataca aatgtaccaa ttaattctgt gatcacattt
    63721 gctggataat tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct
    63781 ttcgttgcat tccaatgtgg caaataagaa agccaaacaa gaatggcccc tgcaaaagct
    63841 cctaaaactt gcgcaatgat gaaaggaacg acaagattcc aactaattgc ccctgtcatc
    63901 gccatagcta gagagactgc aggattcaaa tgagctgggc ccatgaatcc agaaatatag
    63961 acagccattg ttacagcgag tccccaacca aatgcgatga caatccagcc agaagcctgt
    64021 gctttacttt tgtttaaatt cacagccgca cacacaccat ctccaagcaa aactaggatc
    64081 agtgtcccaa taaactcccc catgatttga gtcatatcac tgttcattta tttctccccc
    64141 tttagttccc gcagatctga ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc
    64201 ttctcctctt ctgtccattc aaaatatcca gcaattgcat cgatcacagg ttgcttgatc
    64261 tcatcaagct gatctcgttc aaacaacaga tgatttgtac gacgaatgag ataatcgcct
    64321 ggggccaaaa ccatttcttc ttctaaacca taacgtaacc gagctgactc agcgagactc
    64381 aaaccaggat aaggtgccat ttcttttgct aattcaaaga tacgtcgggc attagtcccg
    64441 taaaaatcag cgatataagt agcatcttct tcctctaaac cggctgctac tccttctttt
    64501 gctaattctg tcactgtttc ttctaattta gttggatcga agtttccacc tgaaatctga
    64561 tattttttag agtcgatttc tttcgtctct attccgtatt cttcttttaa taattggcgt
    64621 atcaatcgca aagctccttc tgccatctta cggtaatcgg tgattttccc accagataaa
    64681 gtcagcaaac catctggttc tctttctaaa gaactacctc tggaaatcgt agaaggtgct
    64741 ttttcatcac ggctgttttc caaatggttc aacacgtctt ctacttcagc acgagaaact
    64801 ttgttttcct tatattcact tactgtatcg acaactttat tgaagctctt gtctgaaatc
    64861 gatccattat ctccaccatt gtaatcagaa ccagaattac caatcaacag tggacgaagt
    64921 cctgcccagc ttgcttcgat atcattcaat gtgatatttg cttctggata gcgatgattg
    64981 atcacatcca atagataatc tacatcttct tgtgtgactt tcggatccgt aaaatctcct
    65041 tggtaatccg tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc
    65101 atccgcccat cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg cagttttttc
    65161 gcatctacaa ctaaatggat ccctttagtt ggacgcattt taggagagac tggacgcgta
    65221 aaattcaagt ttcgtacttt atctacccaa ggaccactcg tattgatcac taattttgct
    65281 ttgatttcga tcacttcatc tgtcagcagg tcacgagctt tgacgccaac gatttgatcg
    65341 ccatcatata aaaagccaac cgctttcatt ttacttacta gataagcccc atcttcggca
    65401 gcctttttga tattatcaat cactaaacgg gcatcattgt tgcggaaatc tagatagaca
    65461 cccgcgcctt ttagcccttc ttttttcaaa aacggttctc tttccaatac ttcttctggt
    65521 gtcagcgtat aattctcata tttggttcct gtcacatttg ccagtttgtc ataaaggtcc
    65581 attgctactt tgaccgagaa catattgaag gttgttgctc cttcgccctc atagattggc
    65641 aaaagcattg gatctggttt tgggatgtgt ggggcaatac tttgtacgac tgcacgttca
    65701 ccaactgtgt cagctactac ttccacatca aatgtcttca gataacgaat accgccatga
    65761 acaagtttgg tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc gatcaatcct
    65821 gttttgattc ctgatgctgc tgactggact gccacaccgg caccagtgat tccgccaccg
    65881 atgatcaaca gatccaattc ttcttgctgc attttttgaa tgctatcttg tcttgtcttg
    65941 tttgaaaaca tatggatttc ctccttattc aaatgcttgg gtagctttta ctgctttttt
    66001 ccaaccgttg taaagttttt ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc
    66061 agcttcataa aattcgcgga tctcgtctgt atctttccaa taaccgacag ctaatcctgc
    66121 taaaaatgct gcccctagcg ccgttgtttc caagttttct gctcgttgga tcggtacatt
    66181 caagatatct gtttggaatt gcatcagata ttcattgttt gctgcaccgc catctacttt
    66241 caatacagga atcttgattc cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg
    66301 ataagcaatc gactgtaaag tagctttaat gatatcttct tttgtcgtac cacgtgtcaa
    66361 gccaaacata gcccctcgtg ctgcctgatc ccaataagga gcacctagcc caacgaatgc
    66421 aggaacaaca taaacctcat cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct
    66481 agctttttgc aacatttgaa gtccatctct cagccattga atcgaagaac cagccacaaa
    66541 aatacttcct tctaaagcat agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa
    66601 taaattattt ttggataatt gcggtttttc accggtattc atgacaataa atgaacctgt
    66661 accataagta ttcttgacca tgcctggttc gaatgccatt tgtccgaata atgcggcttg
    66721 ctggtctcct gccatccctg aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt
    66781 ataaccataa acttctgagt tagaagtgac ttttggtaac atcactcgag gaatatttag
    66841 taaatctaaa atctcctgat cccaatctag ctgatggata ttgaacaaca tcgtccgact
    66901 ggcattcgag taatcagtaa catgtgcttg tccatcggtc agtttccata ctaaccaaga
    66961 atcgatcgtt ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc cttctacatg
    67021 atcaagaatc cagcgtatct tcgttgctga gaaataggca tcgatcacta accctgtttt
    67081 ttcgtggaaa aattcttgat agccttcatt ttttaagtta tctgcaattc cagctgactg
    67141 tctggattgc caaacaactg catgatagat tggacgtcct gtatcttttt cccatattac
    67201 tgttgtttca cgctggttcg ttatgccaat cccagcaatc tgatttggtt tgatccctga
    67261 ttcgatgaac gctccggcaa taactgactg tacagaattc cagatttcat ttgcatcatg
    67321 ttccacccaa ctttcttgcg ggaaatattg cgtgaactct ttttgagaac tgccgatgtg
    67381 tcttcctttc ttgtcaaaga taattgctcg tgagctagtt gtaccttggt caatcgccat
    67441 gatatacgtt gattctgtca ttgtgattcc ctccattata tgcaaacgct ttctttaact
    67501 actcttatca tactactgtt ttaaaaaaag aaactgtcaa aattttattc tttttttctt
    67561 tttttgacag ttttttatta tgaagctatt ttttaatcaa ttcttttctt ttttcagcgt
    67621 ctctattctt cttttcaatc gaataatgtc gtaaggtgac gcaaattctg acagataata
    67681 ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt ctttaccttc
    67741 ttcataactt ccaatctcaa taccgccgat tcccctcagt tctctgatca gatattgttg
    67801 aaaagcaata cgataaatcg gcagactttc taaatcgatt ccaatcacta ctggttttgc
    67861 aatataaaaa tcgatcatca aaagaacatt ggcatactgg atcatcaggt aactaagctc
    67921 actatcttca gctcttttcc tcggcaattg ctgcaccgct gtttcctgta atttcgccag
    67981 taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc gatcacgctc
    68041 atagacttcg atctttccaa caaagaaaaa ccattcattg ttgatctggg aaatttggta
    68101 gcctactgcc ttttcttctt tgattttcaa acgacgagga cgataatgcc ataacatcgt
    68161 ttcccgaatg taagtatcta aaacagcggt tggtaacttt ttgctccgtg tcaaatcata
    68221 ctgataaaat atttcttcgt ctactatcga aaaagaaaca aaaaagctgt aaaacatcat
    68281 tgtttcgtat acattcagtt ctgctgctgt tcgactaaga tgcatcacga tgattggatc
    68341 gattgcttga taaagccggt ccgactgata gagtaatttc ttttctttca aagttgcgta
    68401 cgtacttttt tcattcaata gtcttttttt cgtaatcccg agccagcaag caatctgtgc
    68461 ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa tgaacggtct
    68521 tttttctggt gtattttgca aaaaaagcgg tcgctgatct tccgggatat attggaatag
    68581 ttcataataa aaatatcgta tctgtagctc ttctccgtat aattgaccgt ttttgatttg
    68641 taatccaaat tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa cagaagactc
    68701 actgatatta aacgctgtcg ctaatcgaac gatggatact tgctgatgtt ctaataaata
    68761 gacgagcatc ttaaacttca agctttcctg caataaaaac aatagaattt tttccaaact
    68821 tacatcttcg tccaacacaa gcagcgcctg atactcattt cgctgtactt ctactttttt
    68881 ttccatcatt cggccaatca attcaatatc ttccaaatat tgatcaaacg aggcttttga
    68941 cagttggaca tgctctctca aaccattgat agaggctgtt cccccttgca gaacaaggtt
    69001 tcgcaaaatc aggatctgta actgctcttt tctttctaat aatgcttcaa tcttcatagg
    69061 cagtattcgc cacctttgac cacaccatat ctggcacacg gtattctggc tggaaaaata
    69121 gctcatgttc ttctaactgt tcaaacgtcc atccttcttc tactgcaatt gtcagttggt
    69181 caagaaggtg gaacatctct tttttgcttt ggagctgtgc tccaagaatc tttttttcag
    69241 aatcaaatag aaatttgagt ttaactttgc tttgatttat tgctgtaagc ttgtagtcta
    69301 cgtatcctgt ttcaattttt tggggataaa aaatgctctc ttcttctgtc aaacctacgc
    69361 ttcctaagaa atagccaaac cactgattgc cgactggacg ataggttccc tgatcctttg
    69421 tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt acggacagcg ttatttacta
    69481 gagaaacata cattttttca tttgttggac gaaaagttac ttggatcgca tcgccaatcg
    69541 cataaacgtc tttttgagac gtttgcaagt agtcatctac ccagatcgtt ccatcatcat
    69601 tcaatgttaa ttgttcttcc cacatctgat tgtcgggacg agaatgatta gccagcaaaa
    69661 caagatcata gttctccttt cttttttccg tctctacaat agcggtttca ttttcggtca
    69721 aagacacaac ttgttcttct aaaaaaagtg aaagcccttg atttttcatt tcctttacta
    69781 acggttctgt catttcagga tctagataac gaaaaagaat tgactttcgg ctttcgtaaa
    69841 gatggatttg tttttttctt gatgccaacc cttcagctat ttcgattcct gcttgtcctg
    69901 caccaataat tgcaattttc tttgcattca tgatcttttg ctgaagagcc gttacgtttt
    69961 gtatcttttt aactgggtga ataagcgttg attctgcaga aatatttcga aacttttgat
    70021 ttgaaccagt cgctagaatc aaacgatcaa aatccaattt ttttcctcct gctaaaacga
    70081 cttttttttc tttcaatcct attactgtct tttctgtgta aacatggatt cgatagcggt
    70141 cagtcagttc ttctttcgtg atccaagaaa gttgtttttc actggtaacc tttcctttta
    70201 atatcaaatt gaacccgctg ggaatccatc ctaacttcga ttgcttttca atcaaatata
    70261 tttcacttgt tggcatcaat tggcgaagat aaatagccgc atgaatccct gcaaacgacc
    70321 cgccgacaat tacaatgttc atctgcattc ctctttctta taaggtgttc tactgctaac
    70381 ttctcaatca gaaaacacag tattcataac tcgctttcaa ccttatgata catagcgatg
    70441 cagcagaatc aaccctctcc atcataccgt gttctaggtc tgcttctcaa taatgtacta
    70501 gtggacctaa ttgaagagta tcatttctga agagatgttt caaaaaatgt actcttataa
    70561 aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag gaaacagaaa agaacgaaaa
    70621 gacgagtttt agcgtgtgta atgtaatagt tagaatagac acatcattta tttttaattc
    70681 tttaatgctt caacaactgc ttcgcctaat tgatgagcag attgaggatt ttgccctgta
    70741 agaagccgcc catcaatttg aaccacttct gaaaaagctg ctccagactg atagtttgct
    70801 cctttttctt tcaaagcatc ttctgctaag aatggtactt cattcgttgt accatttgct
    70861 tcctcttctt cattactgaa cccagtcact gtacgtccag ctaaaataga ttttccagac
    70921 tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac cagcccttca
    70981 tttcgataga tttgtgaagc gactttccca agatctattg cttctggaaa atcccacatt
    71041 gttccgtggc caccagcata ataaaccgct gcataatcat ttgcatccac atcatcagga
    71101 cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat cttcataata ggcccaatcg
    71161 atttcatcca tattcaaaac actttgcggt tctaaaggta catatccgcc tttaggactg
    71221 acaaaatctg catcgatccc attttttact aatacatcat aaaaatgagt cagttcactt
    71281 aaccacaaac ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt taatacgatc
    71341 aatactttca ttttcccatc ctctttctat aaaaattgcg ttattgataa cgtgctttca
    71401 agaaattata taatgggctt ggcaaataaa gctctttata tgcttcttca aaaggtttcg
    71461 ttacaaattc atcctcacgg ttttccaata atttctctgc ccagtcagga tcgacgatca
    71521 tttgctgtcc aattgctgca agttctgcat atttcaatac ctgctctaca tcttcgcgat
    71581 tgcggatccc accgacacca actaaaggga tctttccttg aatcgtgtta tggaccactt
    71641 caagaatcga tttatcgttg tatttatcag aacgagctat tcgatcatac atatttaatg
    71701 aaacatgcag ataatctagt tttgactcac gtaatcgttc aagaagccag atcgtgtctt
    71761 caaaacggat acctggtgtt tcatattctt ctggtgagaa acgataccct acgatgaatg
    71821 gttttttagc atattgttca accgctgaga aaaccgttgt taataattct tcaataaaac
    71881 gataacgatt ttccaagcta ccgccccatt gatcctctcg tctattcgaa tgcggagaga
    71941 aaaattgttg gatcaaatac gtatttgctc catgcaactc gattccatca aatcctgatt
    72001 caatcgctct acgtgtggct ttgccaaatg cttggattgt ttcaacgatt tcttcttcac
    72061 tcattgctcg tggtgtttcc gcatcaggac gttttgctgc aactgcactt gcagatacag
    72121 gctgttctcc agataaagtt gctcgacttg tcattcgtcc accgtggaaa atctgaataa
    72181 tcgcttttgc gccttctcct tggattgctt tcgctagctc acttaatcga ggaatcattt
    72241 catcatgagc gatgctgagt tcacctggcc agccttttcc gatatcttgg acatttgcag
    72301 caccagtaat aacagcacca agacctttag aacgttgttt ataataagtg atctcatctt
    72361 cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc atcaatcggt
    72421 tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac attgtcattc
    72481 ctcccataat tcgctctgat taacctatcg agaatcatct atcattaaac cactcgatta
    72541 gttgtacatg caactaattt gcttaaaaaa ttttattttt tttgtaactt atctgttgca
    72601 tcattcattg tctgggctaa ttctttactt gcttcatttc ccaaaactgc tttgtatgac
    72661 tgatgaagtt cttcccagct accatcgact tccttagcaa attggcgtcc ttgttctgta
    72721 agataaacta atgacaagcg accttcgcta actgttttga cgtatccttt atattctaat
    72781 ttttcaacaa aacgagcaat cgttgatggg gcggaataga gcatttgtgc aatatctttt
    72841 tgtggtattc catctttttc ttttaatatc aataaaataa aaccataagt tggtgccaga
    72901 cctgtccttc taaacgcttc atcagctaat ttattcaaca tgcgatctag ctttttcacg
    72961 ctaaagaaaa gacattcatc taacgctttt tcaattggct tttccatttc atcccctcca
    73021 ttggcgtaca tatcatagta tgatttttat tttttgtcaa aaaacacgga ataatgtccg
    73081 cctcctctgt ctagatttga aaaaaagaag tagccatctt caaagaattt cggcttgttt
    73141 tcgacaaaac tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc gtttagtttt
    73201 gagtatgaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa
    73261 taccgaaaaa ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa gccgtcctgc
    73321 atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttgtcgt tttttgagct
    73381 aatgccgatg gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc tgccgttgaa
    73441 ctccgagtca caaggagcaa tttcaaaaaa cagcttctca tgttttatga ctatggtgac
    73501 ttgtgatcga ggagttggca ggagaatact gtttattcgt aaagaagggg ctgtgacaag
    73561 acttttgtca cagtccctta tttcctttta ttataggatt tcgccatttg attggatgac
    73621 ttgtctgtac caatcaaatg attttttctt taaccgtttc tttgtgccat tcccttcgtc
    73681 atcttgatca acatagatat atccataacg ttttgacatt tgtgaagtag aagcgctgac
    73741 gatatcaatg cagccccacg acgtataacc aaataaatct actccatctt tgatagcttc
    73801 cttcatctgc tcaatatgct gcttcaaata gtcaatgcgg taatcgtctt gaacggttcc
    73861 atcttcttct actttgtcta tagctccgag tccgttttca gctatgaaca gtggtttttg
    73921 atacctatca tataattgat tcaaagcaat tctcaaaccc ttaggatcaa tctgccagcc
    73981 ccattcactt gatggaagat acggattttt aattcctcca gttattaagt ttccttggac
    74041 cccttcccaa ttttctggat gagcactgac tgcactagac atgtagtagc taaaagaaac
    74101 aaaatcaaca gggtaggctg ctaagatttc ttcatcacct acttctattt ttaagttgac
    74161 acctttttct tcaaacattc gttgcgtata cgctggataa tatcctcttg cttgaacatc
    74221 actaaagaac aaagaagttc tttgctgctc aattgttttt tgaatatcat ccggatcaca
    74281 agaatacgga acaagaagct gatagctgat catacaaccg acttgtgaac ctttgataat
    74341 ttcatgacaa gcttttgtag ctaaagcact tgctataaac tgatggtgtg caccttggta
    74401 tttcgcttgt tccaaatggg gatggttttc ttcaattact cctacgctaa caaaagggtg
    74461 atgttttaca cagttgatct cattgaaagt catccaatat tttactttat ttttatagcg
    74521 tttgaatact gtttcagcga aacgaacgta aaactgaata agctttcgat taggccatcc
    74581 accatattct gtaaccaaat ataacggcat ttcataatgg gaaagagaaa cgactggctc
    74641 aatcccatat tttagacatt cgtcaaacac ttgatcataa aaaaataatc ccgcttcatt
    74701 gggtgtttct tcatcaccat gaggaaaaat tcttgtccat gcgatagaca tccgatagca
    74761 tttaaaaccc atttcagcaa ataaacggat gtcttcttta aagtgatgat agaaatcatt
    74821 tccgtgtctt ttaggataat aatacttgct gttgggatcc tgcgccgctt ttacttgctg
    74881 atgcgtcatc atatgccatt gatctgtcca tttttcttta ggtaaatcgc tgtcgaatgt
    74941 atacacatca gacacagata atcctttttc atctacatcc catgcacctt cacattgatt
    75001 ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat tttttcatat cgatccccct
    75061 aaacttgcta gttttcttgt ttgattattt ttcttatgac tagtaatgat atttttgaat
    75121 ctactactat ttgtaacaat aaccattgtg gtcggatcat atccctcttt gattaattct
    75181 tgcaaatcta cttccatcaa taaatcgcct ttacgtactt tttttccatc agatactgca
    75241 ctttgaaagt atttgccatt taaattcact gtatcaatac cgatatggat cagtatctct
    75301 gctccttctt cactgactac cccaattgca tgtttcgttg gaaaaactgt tgaaacttcc
    75361 ccgtttacag aactgtagat ttttccgtcc gttggaatga tcgcaaaccc ttctcctaaa
    75421 gatccactag caaatgttgg atcgtttact tcagataaat caattatttc acctgctgca
    75481 ggagtactta acactatatc ctttgaggat tcttcttcga tttcatcctt gtagccgaaa
    75541 agaaaagtta gcatacagga actgatcatc gctaagaaat aactagcaac tagccaaaca
    75601 aatccttcac caatataaat cgggaaagac aaaatacttc ttgtagcaac tgccaaagca
    75661 ccggctcctg cggcaccagc tactccacca aatatagctc cacatataca agcaatgtaa
    75721 aatggtcgtt tatatttaag agtcacacca taaacagcag gttcagtgat tccaaataat
    75781 gctgtgataa atgcagataa agataaactt tttactttct tatttttcgt tttcaaaaat
    75841 accccaagta ctgctcctgc ttgcgcagct acggcagtca tacatgctgg acctagcgta
    75901 tcacgaccgt agaaagaaag attgttggtc attactggta caagtcccca atgtaaccca
    75961 aaaatcacta acacttgcca aaatccgccg ataacagcac cagataaaac agggctaaag
    76021 ttataaacag ctaaatatcc agctgcaatc agctgacttg cataatctgc aattggacct
    76081 acaactaata aaataattgg gaccatcacg atgatacaaa tgaatggtgt caataaattt
    76141 cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag aacccataca
    76201 gctaaaattg ctggaataac cgtacttgta taccttgcta gaatcacggg catccctaga
    76261 aatcgcaagg ttatgctatc actatatgca ctgacaatcg taggataaac gagagctgct
    76321 gcaataacca tagcgatgaa acgatctgta ttgaactttt ttgcagcagt atatgccaaa
    76381 aatataggca agaaatagaa aaatgcatca gctgctgcat tcaaaatgat ataagttcct
    76441 gattcagtag ttaataaatt caaactcgta cataaggcga gtactccttt gatcaaacca
    76501 cttcctgcca agacgcctaa agtcggggca aaaacagcgg cgatcatgtc tataaaagag
    76561 tttaaaatgc cttttttctc agttcctctc tttccactag atgcatcatc ttctagtttc
    76621 atttgtttgg ataatacgtc atacacttca tttactgcat ttcctactac gacttgaaac
    76681 attcctccag cttcgactac agtgatcaca ccttctagtt gctctacctt ctccttatct
    76741 gccttcttat tatcttttag ggtaaagcgt aaccttgttg cacaatggta aacattgctg
    76801 acattttctt cgcctccgac aaatcggaca atatctttcg ctaattgtga atagtccatt
    76861 tttacttccc ccttgttaat taatgccgta gcaatccttc ttacaagata agtatagttc
    76921 ttagaaaacg gtttctccat tcaattagta ccccttctaa tagcgaacga aactgtacaa
    76981 tagtgtgtat ggtttactta gccgagcaaa tcactcactg cttgaaagaa aacaagtata
    77041 tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct ttgctgatgt
    77101 ataagtggta tctcaagctg atactctgtg atttttgttt ctacttggct tttgataaga
    77161 cagcacaaca aagtctcggc atttaacgga agcataacta ggtcagaact aggagagatt
    77221 agttgctgaa tggaatctat ctttttatgg atttttattt gattttcttc tttgtagtaa
    77281 tgatcaagca gttttgtttt ctttttttct tcaactatgg tcagcttttc acgcaaaatc
    77341 tgataaatct cccaagattc aaactcatct agaacactag gtagataaat caaatcatcc
    77401 cactcaaaaa taagctcttg ctcagtagtc aaaattgcat catattccgt tattttgttt
    77461 gattgatttt tgaaaagata cactggttct atccggcatt ctttgatcca catgttcata
    77521 ctttgttgaa tctgtttttt tagtgtattc aaaatactaa ttggctggtt actaacaatc
    77581 agtatctccc cttgttttcg aaaaggattc aacgcttcgg caagatacaa aacaaaataa
    77641 gcgctttcag ctaaattggg acgtaattga aaatgtattg ggaagaaacg ccgaattaaa
    77701 taaatttcta aaggataacg ggagatgatc ttttgtacaa aatgattcat ggtattatgt
    77761 ccggcatcca tcctcgtttt catttgtttg atatgggtta gcaaaaattc tgtttcatgg
    77821 aacaataacg aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa tgctcttaga
    77881 ttcttctcta tttctttgtt tttttcatca ttaatcatca aataatttaa ctctaatagt
    77941 cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat agggtagttc tgatgataac
    78001 ttttttgtga gggtctctac catcggaata aataatgtat acctgtttgt tttttctaaa
    78061 agttcttctc ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat gaagtcctct
    78121 cctgaaacaa tataattata tgatataaga acctctttta atgtcttttg gacagtcggc
    78181 atgatattgg aaaaaattgt actgatttgt tgaggaaacc ttgcgtgttg gatcaccgtt
    78241 tctgttccaa caagagataa gaaaattctt tttgaatttt cttcagcaat gatttttagt
    78301 ccttttgtcg acgaaacttc cagttctaat ttaggattgc gctcgatcca acgcaaaatc
    78361 gtttttattt gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc
    78421 atactgacat accctttttg aaacaataag tacgtcacaa tagttgaagc tcggaaatcc
    78481 atgctggaat agattttatg tttcgtcacc tcttgtatga tttgctgttt agtttcagat
    78541 tcaattgatt ctaaaaaata acctgaacgt ttatttgact ggataatgat tttcttctcg
    78601 tagatagcaa gcatattttt gatttcagtt tggatcgttt tcttgttggt acctaaagct
    78661 tcagctaatt cttcgcttgt tatccaatga ttcttatttc ttaatactac aagaatttgg
    78721 agttgtcgtt tatttaagtc catttgctta cacctcctct ttgttataaa catacaggaa
    78781 aaacctctca aatgacaata gtcacttgca gaggtttaaa aatcatttga agaaagatct
    78841 atattcaagg tttttaccat tagactggat aatttgttta taatattcaa aactatcttt
    78901 tttcaatctc ctaaagcttc cgctcccatc attttgtcta tccacataaa taaagccata
    78961 tcgcttcctc atttcccctg ttcctgccga tacgatatcg attggtcccc accataagta
    79021 accaatgatt tggcaaccat cttttattgc ctcagcaatt tcctgaagat gtatttttag
    79081 atactcacaa cgttttggat cgtggattcg attcaattca tctggtgctt catctagtcc
    79141 aatgccattt tcaactatga ataatggtaa ttcataacgg tctgtcaatt cattcatgac
    79201 ataacgaaac cctagaggat caattggcca actccaaggt tcgggagatt tttccttcaa
    79261 aaagggatta tcgaaaccat ttgctccgcc agtatctacg cgcatttctg cttctttttt
    79321 atagacagcg ctacgataat aactaaatgc aatataatct gccgtgttag cttgcaagca
    79381 ctgttcgtct ccatctaaca ttactggtgc acagtcttct tccttccatg tcctgtaaat
    79441 ataagctgga taatgccctc ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa
    79501 ttgcagtgaa ccaaatacat tctctggatc acagtcatat gcataagtcg ctaatgcaga
    79561 aaagctacac ataataccca tcttagcatt tggatcgatt ttctttaata attgagtagc
    79621 acgggcgttc gctagaaaca tatgatgaca ggcttgataa atcacttttt ttgttagtgt
    79681 agcaatgggt tcaagtttat ctctagcagg gaatgtaacc aatccagcag ctgcataagg
    79741 catacgaaaa gcattattga tctcattaaa ggtcatccaa tatttcactt tcccttggta
    79801 gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg attaattttc gattttgcca
    79861 ccccccatac tctcctacca atgctaaggg ggtttcataa tgactaagag taattactgg
    79921 ctcgatatca tatcgattta atgtattgat tagtctgtca taaaatacga gaccagcctc
    79981 atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa
    80041 agcttttaaa cctaactcct tcagtaaacg aatatcctct tcaaatcgat gataaaaatc
    80101 aatcgcttca tgagaaagat aatggagtga ttcgtctaaa gcaatctccc aaatcttttt
    80161 ttcttcattc cactgaatac taggatgctg gttcatgatc ccgataagcg tatctgtcac
    80221 attaggtaat ttgccgtctt ccaaccatgc accttcacat tgattagcag ctactgctcc
    80281 gccccataaa aaattttctg gaaatatttc ctgatccata actatctctc ctcgctatca
    80341 acaatttctt acttgaagta tagctcgatt catcaaaaca agatattcta actatttcgg
    80401 cagaagagaa gaaaaaaatt gaatgctact cttcattttt aatatttata tataacaaat
    80461 acataagacg atccccgaaa gaaaacactc gttaaccatc taagcaaacg atttatttta
    80521 cttccaacac aaatgcttca taaggtcgta aatcaacttt gccgtctggt aactgtttca
    80581 gaggataatt tgacacaata acccttaaag cttcacgtgg aatagtgaac gtctgctttt
    80641 cactggacat attacaaata acgagccatt gctggtcttc atatatgcga gaataggcaa
    80701 agacatgctc aggcgtcttt tccactaatt catagtctcc ccagacgatg atgtcatgtt
    80761 ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac agaatcttga tctgctaacg
    80821 cttcttctgc attgatttct ttataatttg gattgactcg aagccatgga attccagtag
    80881 taaacccagc atgttcacgg gcgttccatt gcataggcgt cctagcattg tctcttcctt
    80941 tcgccttgat tgattccaag atttcctctt ttgtaaatcc agaagaaatc ctttcgttat
    81001 acatattgat tgtctcaata tcttttgctt ccgcgatatc atcgattggg caattcgtca
    81061 tgcctaactc ttctccttga tagatataag gggtgccttt catcatatgg agcaagatag
    81121 caaacatctt ggcactttct tttcgataca catcgtcatt tccccaacga gaaacaattc
    81181 gcggaagatc gtgattattc caaaataaac tattccatcc ttcatcacct aaagatgttt
    81241 gccatttgga taatactttt ttcagttcag agatactcaa gggtttcaaa tcccatttgt
    81301 ctttgccttc ttgctgatct aatcctatgt gttcgaattg gaagaccatg gataattcat
    81361 ttcttttagg attggaataa agtttagcga tttcggtagt tgctccccat gtttcgccaa
    81421 cagtcatcag atttttgtca ccaaaagttg cttgattcat ttcctgaaga tactcatgca
    81481 gtttaggtcc attacttgtg atttctttat ccggttcttt gccaatcata tcgatcacgt
    81541 ccatacgaaa gcccccaata cctttatcga tccagaaatt catcatttga taaatctctt
    81601 ggcgaacctg tttatttttc caattcaaat caggttgacg cttgctatac aagtgcagaa
    81661 aatattgccc cgttttttca tccaactgcc aagcagaacc actgaatatt gagcgcaatc
    81721 tattaggctc ttttccattg accggatcac gccaaatata atagtcccga taaggatttt
    81781 ctttattttt acgggcctca atgaaccacg gatgttcatc agaagtatga ttcaccacca
    81841 gatccatgat gatacggata tttcttttct ttgcttcttc aatcagttct tccatttctt
    81901 ccatcgtacc gaattctgcc atgattgctt gataattact gatatcatac ccattgtcat
    81961 cattcggaga ctcgtatact ggagaaagcc aaattgcatc aatacctaaa tacgccagat
    82021 aatccaacct ggaaataatc ccccgcaaat ctccaatacc atcttgattt gaatcttgaa
    82081 aacttcttgg atagatttga taaacaactg aatggtgcca ccatttttgt tccatgattt
    82141 tcctcctata aatggatgtt tctatttttt gcttaatcat ttattttagt ctctctcact
    82201 aaaaattgta acgctttcaa atagctattg caccttgtta tcggtaacag aataaaacaa
    82261 aaaaacagct gaggaaattc cctcaactgt tttttgaaag gattgattta tgcagggaag
    82321 aatgctgatt tcacaacttc tttataaacg gtgttcactc agctgtccct tggtattagc
    82381 tcaaaaaacg gcaaaatata agaaactatt ttttcgtttt tttctctaat actcaggcca
    82441 gaacgctgat ttcacaacct ctttatttca cattcaatat ccacatgaat cccacaaata
    82501 caaagaacag accatacatg attggagata cttcttttcc tcttttagct gctaacatgg
    82561 taattggata gaagataaaa cctaatgcaa tcccatctga aatactataa gtcaaaggca
    82621 tgccgatcaa gatcaagaat gaagggatcg caatctctaa gtttccccat tcgatttgtc
    82681 gcaaagattg tgccatcaat actcctacca taatcaatgc tggtgcagtt acttgtgaag
    82741 taacaactga tagtaatgga gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa
    82801 tagctgtgat acctgaacgt cctccaacgg cgataccagc tgatgattct acaaatgcac
    82861 caacaggaga agtaccaaat aaggaacccg caagcatagc tgttgaatca gaagctaaag
    82921 ctttccctac acgaggcatc ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa
    82981 ctaaagttcc tgctgtatca aagaaagtca caagcaagaa tgtcagcact acgacccaca
    83041 tttgaagaga attgatgtcc cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac
    83101 ttggtgctgc ggcaacaatt tcagaaggca ttggaatcaa acctgtgacc agacctaaaa
    83161 tcgttgtagc tgccatcccg atgaagatgc cacctggtac acgccgaaca agcaagatag
    83221 cagttacgac taatccaaaa atcgtcaacc aagtagaacc aacgttgagt gatcctaatc
    83281 ctactaatgt agattcattc gaaacgatga tcccaccttc gctcagacca aggaaagcga
    83341 taaatagacc gataccgcca gaaatcgcat atttcaaatc tgctgggatc gcatcaatga
    83401 tcatttcccg cagcttgaag attgtgatca aaataaaaat caaagaagca acgaatacgc
    83461 cggctaatgc tgtttcccat ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag
    83521 cattgatccc aagagcaggt gccgttccaa ttggataacg ggctaatacc cccattaaaa
    83581 cacagccgat cgcactggct aatgcagtcg cagtaaacac tgctccttca tccattccag
    83641 aagcacctaa tacagttgga tttacaaata agatataagc cattgagata aatgttgtaa
    83701 atcctgccag tacctcacgt tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa
    83761 gtattttttc cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa
    83821 aatttcgatt tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa
    83881 acaaaaaatt cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct
    83941 caaagttcat gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt
    84001 cttcattcta gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct
    84061 tctttgatga attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta
    84121 tcgaccattt ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga
    84181 agcttctcac tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga
    84241 tccactgttt ttcctccttt ttattcaatc agacgactca gcttttccta taacaaaata
    84301 attgccttca gggtcagaaa aattgaaagt caagatgcca ttgttatctg tgatttctcc
    84361 tggttcttcc atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg
    84421 cggtggtcct aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa
    84481 ttctaattct tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt
    84541 ttcagaaatt tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt
    84601 gacataaagc atcacacgtg ttttttgtat catcatttga cccttctttc tatttgattt
    84661 tctttcctgt ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga
    84721 tactattcaa gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt
    84781 tacaaaaaaa aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc
    84841 ttctgattca tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat
    84901 tttttccttc aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga
    84961 ttcaatgacc agcaccgctg cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg
    85021 ccagttgaga cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag
    85081 tgccggagca tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc
    85141 taaatgcaga tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga
    85201 caaggaaata ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg
    85261 atttgcacgt ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt
    85321 ggtaaatgcc tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag
    85381 gtaatttctt gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc
    85441 ttttgttacg tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa
    85501 tacttcccag aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag
    85561 aataattgct tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt
    85621 tcgctgtatt tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc
    85681 caaacaccga gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct
    85741 agtaaaaatg taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt
    85801 tctaaaaaat gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa
    85861 cccgcaggct cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct
    85921 tctgtaagtt tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa
    85981 ttatcttttc caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca
    86041 tctgaaggcg tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc
    86101 cttttattgc cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt
    86161 aaaagatatt ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg
    86221 gagtagttgt tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag
    86281 agtttcttga tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct
    86341 aatttcttga ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt
    86401 tcttggatga ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt
    86461 aaatcgactt ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg
    86521 atgtctcgaa ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttca
    86581 cctctttttg ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac
    86641 tagtaaagta tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt
    86701 tgttttctaa aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact
    86761 cttctatact aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt
    86821 caccgtggat cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt
    86881 tttgtttatt cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt
    86941 cttcatagac ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac
    87001 caggaagtga ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt
    87061 tgatataggc acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg
    87121 cttttatttc ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc
    87181 ggatctcctc aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag
    87241 aaagtgcgag tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt
    87301 tagcaaatgt atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat
    87361 ataatgaaat ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga
    87421 cttctctggg gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca
    87481 ccaaacgaaa aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac
    87541 agctttttca caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc
    87601 acaatattca tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct
    87661 gaacgacagg ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa
    87721 gccatttctt tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt
    87781 gtaatccact tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt
    87841 gtcatatttt cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc
    87901 tttcgttcga tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg
    87961 acgatctgat tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt
    88021 taggcgaatc gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt
    88081 ttgcttatca ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa
    88141 atcaagtgct ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc
    88201 caaattgtta aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc
    88261 atacatatct ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg
    88321 actaccagcc agaagaatga aatacttttt catctcattt atttcctaag ttaattggac
    88381 tttccaagtt cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg
    88441 ctggaatcac attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag
    88501 ctactttcgt ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat
    88561 tgatcaattc aggataatct tcttctttca cacctgttcc tccatggata actaggggga
    88621 catcaactac tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga
    88681 attgtccatg attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa
    88741 atttcactgc gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt
    88801 cttctgtacc gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat
    88861 attctactac tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat
    88921 acatgacaga agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt
    88981 ggtctaagtg caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga
    89041 ctaacacatg catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg
    89101 aacccatttc ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa
    89161 aagctccaac tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat
    89221 acatctttta ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca
    89281 atttgtcgca tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca
    89341 cgagcagcat ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg
    89401 atcgtctgtt tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac
    89461 aattcagaaa atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg
    89521 gtagtggaaa tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct
    89581 ggcgataggg ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta
    89641 aaaaatccaa acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag
    89701 atccaggcat ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgttg
    89761 aatcccatcg aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca
    89821 tgagcaatac cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct
    89881 gctgtccctc cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac
    89941 atgggcaaag taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg
    90001 acagcatttg ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga
    90061 actaatttca tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca
    90121 ccagctagca atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg
    90181 atatagtttt gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg
    90241 atgatatata gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg
    90301 aaatgtgtcg gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa
    90361 agcttagcaa caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata
    90421 tcatttgatc cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg
    90481 aaacaagctc cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa
    90541 tagccaaaga ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg
    90601 tacttccatt ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc
    90661 agtgcaatat ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata
    90721 gaagggatga tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa
    90781 cttccaacga tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac
    90841 gtgccagaga tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc
    90901 aatgcaatgg tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc
    90961 atgtt
//
LOCUS       DAJSVF010000002        80886 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSVF010000002 DAJSVF010000000
VERSION     DAJSVF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.002"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            192..530
                     /locus_tag="ON550_000082"
     CDS             192..530
                     /locus_tag="ON550_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953200.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            624..854
                     /locus_tag="ON550_000083"
     CDS             624..854
                     /locus_tag="ON550_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953201.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            1254..1625
                     /locus_tag="ON550_000084"
     CDS             1254..1625
                     /locus_tag="ON550_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3953202.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(1899..2591)
                     /locus_tag="ON550_000085"
     CDS             complement(1899..2591)
                     /locus_tag="ON550_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3953203.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(2594..3379)
                     /locus_tag="ON550_000086"
     CDS             complement(2594..3379)
                     /locus_tag="ON550_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3953204.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            3616..5016
                     /gene="lacG"
                     /locus_tag="ON550_000087"
     CDS             3616..5016
                     /gene="lacG"
                     /locus_tag="ON550_000087"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3953205.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5041..5358
                     /locus_tag="ON550_000088"
     CDS             5041..5358
                     /locus_tag="ON550_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3953206.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            5371..7044
                     /locus_tag="ON550_000089"
     CDS             5371..7044
                     /locus_tag="ON550_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3953207.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            7058..8689
                     /locus_tag="ON550_000090"
     CDS             7058..8689
                     /locus_tag="ON550_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR3953208.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            9561..10751
                     /locus_tag="ON550_000091"
     CDS             9561..10751
                     /locus_tag="ON550_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCR3953209.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            10885..13074
                     /locus_tag="ON550_000092"
     CDS             10885..13074
                     /locus_tag="ON550_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3953210.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            13071..14864
                     /locus_tag="ON550_000093"
     CDS             13071..14864
                     /locus_tag="ON550_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3953211.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            15064..15378
                     /locus_tag="ON550_000094"
     CDS             15064..15378
                     /locus_tag="ON550_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3953212.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            15399..15776
                     /locus_tag="ON550_000095"
     CDS             15399..15776
                     /locus_tag="ON550_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3953213.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            15786..16559
                     /locus_tag="ON550_000096"
     CDS             15786..16559
                     /locus_tag="ON550_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953214.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            16581..17984
                     /locus_tag="ON550_000097"
     CDS             16581..17984
                     /locus_tag="ON550_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3953215.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            18166..19350
                     /locus_tag="ON550_000098"
     CDS             18166..19350
                     /locus_tag="ON550_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3953216.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            19347..19637
                     /locus_tag="ON550_000099"
     CDS             19347..19637
                     /locus_tag="ON550_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953217.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            19634..19855
                     /locus_tag="ON550_000100"
     CDS             19634..19855
                     /locus_tag="ON550_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953218.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            19897..20676
                     /locus_tag="ON550_000101"
     CDS             19897..20676
                     /locus_tag="ON550_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCR3953219.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            20743..21237
                     /locus_tag="ON550_000102"
     CDS             20743..21237
                     /locus_tag="ON550_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3953220.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            21293..21931
                     /locus_tag="ON550_000103"
     CDS             21293..21931
                     /locus_tag="ON550_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953221.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            22003..22398
                     /locus_tag="ON550_000104"
     CDS             22003..22398
                     /locus_tag="ON550_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3953222.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            22382..24835
                     /locus_tag="ON550_000105"
     CDS             22382..24835
                     /locus_tag="ON550_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3953223.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            24832..26859
                     /locus_tag="ON550_000106"
     CDS             24832..26859
                     /locus_tag="ON550_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953224.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            26856..27878
                     /locus_tag="ON550_000107"
     CDS             26856..27878
                     /locus_tag="ON550_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3953225.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            27895..28824
                     /locus_tag="ON550_000108"
     CDS             27895..28824
                     /locus_tag="ON550_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3953226.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            29069..29185
                     /locus_tag="ON550_000109"
     CDS             29069..29185
                     /locus_tag="ON550_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3953227.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            29201..30004
                     /locus_tag="ON550_000110"
     CDS             29201..30004
                     /locus_tag="ON550_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3953228.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            30239..30475
                     /locus_tag="ON550_000111"
     CDS             30239..30475
                     /locus_tag="ON550_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953229.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            30482..32434
                     /locus_tag="ON550_000112"
     CDS             30482..32434
                     /locus_tag="ON550_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR3953230.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(32506..33003)
                     /gene="dfrG"
                     /locus_tag="ON550_000113"
     CDS             complement(32506..33003)
                     /gene="dfrG"
                     /locus_tag="ON550_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR3953231.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            33365..34405
                     /locus_tag="ON550_000114"
     CDS             33365..34405
                     /locus_tag="ON550_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3953232.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            34503..34691
                     /locus_tag="ON550_000115"
     CDS             34503..34691
                     /locus_tag="ON550_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3953233.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(34749..35102)
                     /locus_tag="ON550_000116"
     CDS             complement(34749..35102)
                     /locus_tag="ON550_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953234.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            35633..36055
                     /locus_tag="ON550_000117"
     CDS             35633..36055
                     /locus_tag="ON550_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3953235.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            36052..36282
                     /locus_tag="ON550_000118"
     CDS             36052..36282
                     /locus_tag="ON550_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953236.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            36780..36980
                     /locus_tag="ON550_000119"
     CDS             36780..36980
                     /locus_tag="ON550_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3953237.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            37007..38200
                     /locus_tag="ON550_000120"
     CDS             37007..38200
                     /locus_tag="ON550_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3953238.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(38268..39833)
                     /gene="guaA"
                     /locus_tag="ON550_000121"
     CDS             complement(38268..39833)
                     /gene="guaA"
                     /locus_tag="ON550_000121"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3953239.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(39892..40815)
                     /locus_tag="ON550_000122"
     CDS             complement(39892..40815)
                     /locus_tag="ON550_000122"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3953240.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            41011..41616
                     /locus_tag="ON550_000123"
     CDS             41011..41616
                     /locus_tag="ON550_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3953241.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            41924..42940
                     /locus_tag="ON550_000124"
     CDS             41924..42940
                     /locus_tag="ON550_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3953242.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            43081..44382
                     /locus_tag="ON550_000125"
     CDS             43081..44382
                     /locus_tag="ON550_000125"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR3953243.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            44428..45090
                     /gene="deoC"
                     /locus_tag="ON550_000126"
     CDS             44428..45090
                     /gene="deoC"
                     /locus_tag="ON550_000126"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3953244.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            45111..45509
                     /locus_tag="ON550_000127"
     CDS             45111..45509
                     /locus_tag="ON550_000127"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3953245.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            45575..46657
                     /locus_tag="ON550_000128"
     CDS             45575..46657
                     /locus_tag="ON550_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3953246.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            46869..48431
                     /locus_tag="ON550_000129"
     CDS             46869..48431
                     /locus_tag="ON550_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953247.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            48424..49557
                     /locus_tag="ON550_000130"
     CDS             48424..49557
                     /locus_tag="ON550_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953248.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            49557..50513
                     /locus_tag="ON550_000131"
     CDS             49557..50513
                     /locus_tag="ON550_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953249.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            50612..51778
                     /gene="deoB"
                     /locus_tag="ON550_000132"
     CDS             50612..51778
                     /gene="deoB"
                     /locus_tag="ON550_000132"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3953250.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            51802..52620
                     /locus_tag="ON550_000133"
     CDS             51802..52620
                     /locus_tag="ON550_000133"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3953251.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            52637..53344
                     /gene="deoD"
                     /locus_tag="ON550_000134"
     CDS             52637..53344
                     /gene="deoD"
                     /locus_tag="ON550_000134"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3953252.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(53642..54328)
                     /gene="gpmA"
                     /locus_tag="ON550_000135"
     CDS             complement(53642..54328)
                     /gene="gpmA"
                     /locus_tag="ON550_000135"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3953253.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(54500..55081)
                     /locus_tag="ON550_000136"
     CDS             complement(54500..55081)
                     /locus_tag="ON550_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3953254.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(55214..55897)
                     /gene="rpiA"
                     /locus_tag="ON550_000137"
     CDS             complement(55214..55897)
                     /gene="rpiA"
                     /locus_tag="ON550_000137"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR3953255.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKGFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            56216..56629
                     /gene="rpsL"
                     /locus_tag="ON550_000138"
     CDS             56216..56629
                     /gene="rpsL"
                     /locus_tag="ON550_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR3953256.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            56713..57183
                     /gene="rpsG"
                     /locus_tag="ON550_000139"
     CDS             56713..57183
                     /gene="rpsG"
                     /locus_tag="ON550_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR3953257.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            57277..59364
                     /gene="fusA"
                     /locus_tag="ON550_000140"
     CDS             57277..59364
                     /gene="fusA"
                     /locus_tag="ON550_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3953258.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            59536..60723
                     /gene="tuf"
                     /locus_tag="ON550_000141"
     CDS             59536..60723
                     /gene="tuf"
                     /locus_tag="ON550_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3953259.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            61129..61437
                     /gene="rpsJ"
                     /locus_tag="ON550_000142"
     CDS             61129..61437
                     /gene="rpsJ"
                     /locus_tag="ON550_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR3953260.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            61468..62097
                     /gene="rplC"
                     /locus_tag="ON550_000143"
     CDS             61468..62097
                     /gene="rplC"
                     /locus_tag="ON550_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR3953261.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            62125..62748
                     /gene="rplD"
                     /locus_tag="ON550_000144"
     CDS             62125..62748
                     /gene="rplD"
                     /locus_tag="ON550_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR3953262.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            62748..63038
                     /gene="rplW"
                     /locus_tag="ON550_000145"
     CDS             62748..63038
                     /gene="rplW"
                     /locus_tag="ON550_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR3953263.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            63077..63910
                     /gene="rplB"
                     /locus_tag="ON550_000146"
     CDS             63077..63910
                     /gene="rplB"
                     /locus_tag="ON550_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR3953264.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            63953..64231
                     /gene="rpsS"
                     /locus_tag="ON550_000147"
     CDS             63953..64231
                     /gene="rpsS"
                     /locus_tag="ON550_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR3953265.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            64254..64601
                     /gene="rplV"
                     /locus_tag="ON550_000148"
     CDS             64254..64601
                     /gene="rplV"
                     /locus_tag="ON550_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR3953266.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            64615..65271
                     /gene="rpsC"
                     /locus_tag="ON550_000149"
     CDS             64615..65271
                     /gene="rpsC"
                     /locus_tag="ON550_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR3953267.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            65274..65708
                     /gene="rplP"
                     /locus_tag="ON550_000150"
     CDS             65274..65708
                     /gene="rplP"
                     /locus_tag="ON550_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR3953268.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            65698..65886
                     /gene="rpmC"
                     /locus_tag="ON550_000151"
     CDS             65698..65886
                     /gene="rpmC"
                     /locus_tag="ON550_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR3953269.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            65911..66177
                     /gene="rpsQ"
                     /locus_tag="ON550_000152"
     CDS             65911..66177
                     /gene="rpsQ"
                     /locus_tag="ON550_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR3953270.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            66235..66603
                     /gene="rplN"
                     /locus_tag="ON550_000153"
     CDS             66235..66603
                     /gene="rplN"
                     /locus_tag="ON550_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR3953271.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            66640..66948
                     /gene="rplX"
                     /locus_tag="ON550_000154"
     CDS             66640..66948
                     /gene="rplX"
                     /locus_tag="ON550_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR3953272.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            66976..67515
                     /gene="rplE"
                     /locus_tag="ON550_000155"
     CDS             66976..67515
                     /gene="rplE"
                     /locus_tag="ON550_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR3953273.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            67534..67719
                     /locus_tag="ON550_000156"
     CDS             67534..67719
                     /locus_tag="ON550_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR3953274.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            67755..68153
                     /gene="rpsH"
                     /locus_tag="ON550_000157"
     CDS             67755..68153
                     /gene="rpsH"
                     /locus_tag="ON550_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR3953275.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            68185..68721
                     /gene="rplF"
                     /locus_tag="ON550_000158"
     CDS             68185..68721
                     /gene="rplF"
                     /locus_tag="ON550_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR3953276.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            68882..69238
                     /gene="rplR"
                     /locus_tag="ON550_000159"
     CDS             68882..69238
                     /gene="rplR"
                     /locus_tag="ON550_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR3953277.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAKAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            69259..69759
                     /gene="rpsE"
                     /locus_tag="ON550_000160"
     CDS             69259..69759
                     /gene="rpsE"
                     /locus_tag="ON550_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR3953278.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            69774..69953
                     /gene="rpmD"
                     /locus_tag="ON550_000161"
     CDS             69774..69953
                     /gene="rpmD"
                     /locus_tag="ON550_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR3953279.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            69997..70437
                     /gene="rplO"
                     /locus_tag="ON550_000162"
     CDS             69997..70437
                     /gene="rplO"
                     /locus_tag="ON550_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR3953280.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            70437..71732
                     /gene="secY"
                     /locus_tag="ON550_000163"
     CDS             70437..71732
                     /gene="secY"
                     /locus_tag="ON550_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR3953281.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            71794..72441
                     /locus_tag="ON550_000164"
     CDS             71794..72441
                     /locus_tag="ON550_000164"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR3953282.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            72632..72850
                     /gene="infA"
                     /locus_tag="ON550_000165"
     CDS             72632..72850
                     /gene="infA"
                     /locus_tag="ON550_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR3953283.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            72883..72999
                     /gene="rpmJ"
                     /locus_tag="ON550_000166"
     CDS             72883..72999
                     /gene="rpmJ"
                     /locus_tag="ON550_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR3953284.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            73017..73382
                     /gene="rpsM"
                     /locus_tag="ON550_000167"
     CDS             73017..73382
                     /gene="rpsM"
                     /locus_tag="ON550_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR3953285.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILANAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            73410..73799
                     /gene="rpsK"
                     /locus_tag="ON550_000168"
     CDS             73410..73799
                     /gene="rpsK"
                     /locus_tag="ON550_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR3953286.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            73879..74817
                     /locus_tag="ON550_000169"
     CDS             73879..74817
                     /locus_tag="ON550_000169"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR3953287.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            74845..75225
                     /gene="rplQ"
                     /locus_tag="ON550_000170"
     CDS             74845..75225
                     /gene="rplQ"
                     /locus_tag="ON550_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR3953288.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            75497..76336
                     /locus_tag="ON550_000171"
     CDS             75497..76336
                     /locus_tag="ON550_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3953289.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            76312..77181
                     /locus_tag="ON550_000172"
     CDS             76312..77181
                     /locus_tag="ON550_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3953290.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            77171..77968
                     /locus_tag="ON550_000173"
     CDS             77171..77968
                     /locus_tag="ON550_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3953291.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            78171..78917
                     /gene="truA"
                     /locus_tag="ON550_000174"
     CDS             78171..78917
                     /gene="truA"
                     /locus_tag="ON550_000174"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3953292.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            79582..79819
                     /locus_tag="ON550_000175"
                     /pseudo
     CDS             79582..79819
                     /locus_tag="ON550_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(79963..80739)
                     /locus_tag="ON550_000176"
     CDS             complement(79963..80739)
                     /locus_tag="ON550_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3953293.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT        26545 a        14095 c        16723 g        23523 t
ORIGIN      
        1 ttaaggagga acttaaagta gaatgtaaat gaatagttgt agtcagtcga ttgtgatact
       61 agactcaaag ttgcaggaat aagtttttca atttgtgatt ggaagataaa aaatgaaaaa
      121 gaagggcttt tttttatact ttgttgattg tagtgtttat tttccctctg tttagccaag
      181 taacattttc tatgactcaa ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta
      241 cagaagagtt taagaaatat caggaaaata ttgttccgga gactccatta gctagtgagc
      301 caattactga tattgatgga aaccaaatag gatggtatat acagtacgaa gttatggtgg
      361 taaataatga ctcaatagta aaaaatacag taggcttaga gctaacaccg attgaaaagg
      421 tgagttcatt gttaacgtat atttatactg atgaaagtga cgagactgag atcattttaa
      481 cggattactc aaaaattgct actgataaaa agtatatatt gtggatgtag acactagtga
      541 aatactttat gatatagaaa ttgatgaaaa tttactagat gtagcacaag aaatcgatca
      601 tcaaaagcag gaagctattc gatatgctgg tagaaaaggg aaaagaaaat atttattacg
      661 tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa agaattcaag agtcgctact
      721 caattaactc gactccgaca tttacagttt acagagaagg atcgatagaa aaaactgttt
      781 tttggactaa agaaagtgga atgtctttag ctgaagtaga agagtttttg gattatgtta
      841 gtatgcaaca gtaagggagt tcttattatg aaaaattttg acacatatta tgttattgtt
      901 ttttcgatat ctttgctgtt aatattacta actttaatgc agtataaaga gtatccagag
      961 tattttattt taacttttgt atatttagta agtagctaca cacttctata taaattcaga
     1021 agttcttcta gagtttctaa agtttgtttc ttaattagct tattgattct ataatatttt
     1081 tgatttatat aggaaattaa aaatattata gaatcaacac tcagagaacg ggttggtttt
     1141 taatagattt aataagtact gataatgaaa tttctcctgg ttgggaaaac gaagttggtt
     1201 accctgtaga agaatttttt caagattact ttgcggagta tgccgatagg ataatgagag
     1261 attacgtaac agataaaact caagaagtca tcaatcaatc ccagtcatta aatgaattat
     1321 ctgataaggc gaaagaagaa gcagttgcca ttatttcttc agggattagt gactatattg
     1381 atatgagtgg tgaaatcaat tatggcgata aagacaaaat tgtacgcatg ataaaagctt
     1441 ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa tgatacttac ctagtaggaa
     1501 aattaattaa tatccttggg acagagaaag atacggaaac gtttatttcc tctttgtcta
     1561 tgttgtttac agataagcat ttttcaactc agcttggtgg agaggaacta gtggaaatct
     1621 tttaaccatt acgcccagca ttaataaaag tatatcaaaa aagtactcct gatgattttt
     1681 aagattggtt tgagaaataa tgtgaaaaca ctatttttaa tgttaattat agatagagat
     1741 gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat acactgctat atttcactaa
     1801 ggatatagca gtgtatcttt catgtcgttt cactggcagt tttttataaa aggaggtgtg
     1861 catttcagtt gcttttcttt ggtagttagt tgaatcgttt aagaattaac ggatgtggca
     1921 gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg ttccatcttt atttagctgt
     1981 gcctgttcaa atactgtttt gtttttaccg aatggatcaa ttgcaataat tgttccgatc
     2041 cccgcacgtt tggcagcaag taatcctgag taagcatctt cgataacaag acaatcagcc
     2101 ggagctaagc tcagtttgtc tgccgcttta agaaaaatat ccggttgcgg tttcccaggg
     2161 aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata
     2221 tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg caatcgttaa tggaatctgt
     2281 tggtcagtca gctggtcaag agtcgcttca agcccttttg tcaactgaag ctcgttttca
     2341 ttttgcaagc aaagcatacg ataatagctt tctttttctt ctgacaatac ggcgatctca
     2401 gaggcggtca aatgctctga aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga
     2461 atatttttta aaatatcctc gtctgataaa ccatcttttg tatgatcatg tatcatatgc
     2521 agccaagcag cttcatgcag atgagagtcc aaaaacattg ttccgttgaa atcaaatata
     2581 atccctttca tagttacgct cctatagtgg tattttcggt tgaatcagca atttgcagat
     2641 cgacatattg tgcatagtgc tcttgaacct cccgcgagat ctggcgatcc gtaatgagca
     2701 gatcgaaatc atgcaaatta taaaacacat aaaaatcatc tcgattgaat tttttattat
     2761 caacgagtag atatttctta cgggaattat ttagagcgat ctgctgtgct tctccttctt
     2821 cggtactgta agtagagatc gcttcgttgt gaacgccatt tgaactgata aatgcttttg
     2881 taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt
     2941 tccggaaatc accgccaatc agtagaattt ctggtccatc tgcttgagca agaatatcaa
     3001 aaactggata attatttgta atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata
     3061 gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg
     3121 ctgtggcagc aatttctttt ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa
     3181 gttcttgatc taaagtgtag gaaagacttt gagcccctcc atggacacgc agcagcttgc
     3241 ccgatttttc taattcatct aaatctctgc gaacggtcat atcagaaaca tctaattgat
     3301 tgatgatttc attaacggta aggattcctt ttttattgac catttctacg atagtaagca
     3361 atctttcttt tttcaacatt gtcttcacct ctaatttaag gataatctat catataaacg
     3421 gacaacagtc aatctaattc gaacaaaagt aaacatttag ggtggttttt atgtttgtta
     3481 caatagattt tattgctgtt ttctggtttt tatgattatt tgtgtttgtt tttgttgaca
     3541 aatgttttgg atgcgtttac aatgcaatca gttagaaaga gcgaaaactg attatagatt
     3601 tgaggagaga tgtacatgct taaattacca gaagatttta tttttggcgg agccacagca
     3661 gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac
     3721 tttttagagg aacaagggcg attcagtccc gatccggcaa gtgattttta tcatcaatat
     3781 gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg gtctaagatt atccattgct
     3841 tggagccgca tttttcctga tggagctggg aaaccaaatc cagaaggaat cgctttttat
     3901 catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat
     3961 tttgataccc ctaaacgttt gtttgaccat ggagattttt tgaatcgaga aacaatagaa
     4021 gcatttgttt cctatgccat tttttgtttt catgaattca aagaagtcaa ggtgtggagt
     4081 acctttaatg aaatctatcc agttgcaacc aatcaatatt tgttaggtgt tttcccacca
     4141 ggaatcaaat atgattttac aaagatcgtc gcctgtcttc ataatatgat ggttgctcat
     4201 gcccgtgtag tgaactattt caaagaaaac gaacttccgg gtgaaattgg tgtagttcac
     4261 tcattagaga cgaaatatgc ggcaacagac gcgccggaag ataagcatgc tgccttttta
     4321 gacgatgcat tgtcgatccg tttcttatta gatgctacct atttaggcta ttattcaact
     4381 gaaaccttga ctgctcttga tgagatatgt gaagcaaatc aggccagcta tcattttcct
     4441 gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta atgactactt agggatcaat
     4501 cactaccaat gccattttgt caaagcttac gatggagaaa atgcaatcca tcataacggt
     4561 acgggtgaaa aagggacctc tgtttataaa gtgaagggaa tcggagaacg tatttataaa
     4621 gaaggaattc ctcgaactga ttgggactgg ctgatttatc cagaaggtct atatgacttg
     4681 ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa tctatatcac tgagaacggg
     4741 atggggtata aagatcagtt tgaagatggc attattatgg atcagccgag aatcgactat
     4801 ttgagagtct atttggaatc cttaagcaag gcgataacag ccggggtaaa tgtgaagggg
     4861 tattttttgt ggtcgcttat ggatcttttc tcatggacaa atgggtataa caaaaggtat
     4921 ggcttgtttt atgttgattt tgaaacccag aagcgttacc caaaagaatc agcttactgg
     4981 tacaagttgg tcagtgaaac caaaacgatt atttaataag tgataatgga ggaagaaaag
     5041 atgaataaag aggaattaca aatgcttggt tttgagatcg ttgcatattc aggcgacgct
     5101 cgcagtacat tattgaaatt attgaaagaa gtaagacaag gaaactttga acatgttgat
     5161 tcggcgttga aagaagccga cgaaaattta acccttgcac ataattcgca aacaaaaatt
     5221 ttagcggaag aagcatctgg gaaagagatg gagatgggat tcatttttat ccatgggcag
     5281 gatcatttaa tgaccacttt attgctgaga gatctgattc aagactttat tgtactgtac
     5341 agacagaatc aaggatagga gaagaacgct atggatggaa tcattaaaca aatcgaaaaa
     5401 gggaaaccat tttttgaaaa aatctcacgt aatagttatt taggagccgt tcgtgacgga
     5461 tttttaacag cgatgccagc tattttattt tcaagcattt tcattttgat tgctgcggtt
     5521 cctgagattt ttggttacca atggcctgaa tctgtctcca catggctatg gaaagtctat
     5581 aattattcga tgggtgttgt agggttactc gtttcagcaa ccactgctcg ctgcttggct
     5641 gattcgatga atcgtcgctt accgaaaaac aaagtaatca acaccacctc tgtgatgctg
     5701 gcctctgtgg taagtttcct gttgttaagc gtctctccga ttgaaggtgg attttcaacg
     5761 gaatatatgg ggacaaaggg actactggct tcctttgtcg ctgcattttt aacagttaat
     5821 ttatataagt tttgtgtgct gaaagatatt accattaaaa tgcctaaaga agttcctgga
     5881 acgatttcgc aaaccttccg agatattttt cctttctctt tttcagtatt tgcggccgtt
     5941 ttaattgatt taggcattcg acaaatcttc caaatctcat ttgcagaagc aatcattacg
     6001 gtgctgcagc cgctatttac agcagctgac gggtacttag gaatcgctat tatctgggga
     6061 gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt
     6121 gcggcaatta tctatgccaa tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa
     6181 gcttctaaag tactgacggt gggtttaggg aattttgtag gaactatggg aggaactggg
     6241 gcaacattgg ttgttccttt tatcttccta ctctttgcta agtctaagca gttgaaggca
     6301 gttgggaaag cttcctttat tccagtaagt ttcgctgtca acgaaccatt gctatttgcg
     6361 gcacccatga ttttgaaccc ttattttttt attccatttt tgttaacacc tgttgtaaac
     6421 gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga acagctttat gtacgttttg
     6481 ccttgggcaa ctccagcacc gatcggtttg gttctaggaa cgggcatggg tatcttagct
     6541 tttgttctag cagcggttct aatcgtcgtt gacttcctga tctatctacc attttgcaaa
     6601 gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag
     6661 gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg cagcgagtca agttatcagc
     6721 gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg gcgcagggac aagtgcgatg
     6781 ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag caccgatcag tgcaatggcg
     6841 ggtgcttatg gttctcatta cgaaatgctg caggattttg atatggtggt cttagcaccg
     6901 caagtcaatt cttactatga tgatatcaaa gaagacaccg atcgcttagg aatcaagctg
     6961 gttgcgacaa aaggagcaga atatatcgga ttaacgagag atcctgaaaa agctgttcag
     7021 tttattctga accaatttca ataaaggagg agccataatg aaaatgggaa tttggttgcg
     7081 taaagctgtt ttatttgcag ctgtgttagg ggctttgggg gctgtttcaa aaactgttga
     7141 agcatctgcc agcgaattta ttcgcggagc agatatttcg atcctgtcag atatggaaaa
     7201 atctggtgca aaatattatg aagacgatat ttcaaaagat gccttagaga ttttaaaaaa
     7261 taatggtgtc aactatgttc gactgcgttt gtggcaggat ccttataata gtaatggaga
     7321 aagttatgga gcagggacaa atgatctgga aacgacgatt gaccttgcaa aacgagcaaa
     7381 aaatctggga cttaaggtgc tgctggattt tcactacagt gatttttggg ttgatcctgg
     7441 gaaacagaat cttcctaaag catggcaagg tctgaccttt gaagaaatga atacggcatt
     7501 gtacgactat acaaagaacg ttctgtcgga aatgaagcag ctggacgttt atcctgatat
     7561 ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg ccttatggca agtcttgggg
     7621 agaaggcggg ggagaatttg atcgattggc tgcttttttg aagtctggta tccaagctgt
     7681 tcgagacact caacctaaaa caactccagt aatgctccac ttagctgatg gtggcgatac
     7741 aggagcattt acttggtggt ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat
     7801 tggagtgtcg tattatccgt attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa
     7861 tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt gaaacagctt atgggcatac
     7921 aacagcgaat gccgatacta tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg
     7981 atatgaacca acaccgacag gacaagcaga gtatttgctg gatcttgccg atcggattca
     8041 ggcagtaccg aataatcgcg gggcgggctt cttctattgg gaacctcttt ggtacaacgg
     8101 aaatgtgagt tgggcaacac aagcaggtat gaattatctc agtgttcaat ccactatggg
     8161 aaacgaatgg gacaaccaag cgatgtttga ttttgctgga aacgccttgc caagcttgcg
     8221 tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga
     8281 tggaccaacg accagtcctt ccaattggca aacctggttt caacaaggaa ccgctaaggg
     8341 tacgatcaaa acggaagcgg gcactttttt tggggactac aaactaactt tttggcagga
     8401 tcaagcatac gaggcatcag cttatcaaaa tgttagtggc ataagcaaag gaacttacac
     8461 attgagtgcc tatgtaatgt ctgggggaga gtttgatact aatcagttat atatcaaaaa
     8521 ttatggtgga gcggaactaa atactgcggt tgttaaaaca agtgaatgga caaaaattac
     8581 aatcaaagac attccaatta caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc
     8641 aggaaatgct tggacgaacg ttgattttgt ttcattggtg aaacaataga ctgcggatcg
     8701 tttcgattca agatcggagg ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg
     8761 aacctgcacc tcgcctttgc caaaaataac cagaaacgat ttccagtcat ttttaccaaa
     8821 acgagtaaag caccagactt catctacact ttgtattatg ctaaagagag ttcaacgatt
     8881 tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga taacataaag tttgtttaag
     8941 aaagggtaac cttagaattc cgtcgtgaat atgatttgag cacttagatg gatagaaaaa
     9001 agttctcgga aaacagttcg tttttcgaga acttcttttt atgataagaa tgaaacaaat
     9061 taacttatat tatagtctct tttggtaggg aaccatctta tttttaatga aaatcaagca
     9121 ttttagctat tgaagaatgt tgatttatag ggattccccc tccatattat gttggctctt
     9181 tattcccact ctgttttcaa cgtaaagtgt ataaaacaga atgcgatact atcaagggag
     9241 ttaaaacaat atacagaaca gtaaaacact ataaataaaa ataaaagtgc aataaaagtg
     9301 caatttttgc acctaatcat actctgttta tatatgaaaa ctagattctg aatccaatat
     9361 ttggttaacg agaggcgttc gaatttgaac gtcttttata tattactttc aaataaatat
     9421 gaggtagaat actatctatt ttctttttaa cccaattgtt ataatggaga gtgtaaaata
     9481 ttttgtgtaa atagaaaaaa ggaagtccct tctgtagaat agagttacca caacacattc
     9541 acagaagaga ggacttccct atgaacgatt ttactacaga aattctaaag actctagcga
     9601 acaaaggcga tttgaatgaa ttattccgtg tccatttgga gaaagcagtc aatacgcttc
     9661 tcaaaacgga gttaacggct ttcctagatt acgaaaagta tgatcgcatt ggttttaaca
     9721 cgggtaattc tcgtaacggc tcctatgacc gtacggtcaa gaccgagtat ggggaacttc
     9781 atctccagat tccgcgcgac cgcaatggtg agttcaagca acagactgtt cctgcttata
     9841 gacggacgaa tgacacgtta gaggagaccg tcattcacct cttccgaaaa ggtattacca
     9901 tgtcggaaat cgcagacttg attgagaaaa tgtatgggca ttactacacg ccccaaacca
     9961 tgtccaatat aacaaaatca tttacagaag aggtaacggc gtttaatggg cgggagcttc
    10021 atgaccgtta tgctgctatt tatatggacg caacgtatat tccgttaaag cggaaaaccg
    10081 tcgccaagga agctattcat atcgcagttg gcattcgccc ggacggatca aaggaagtat
    10141 tgagctatgc gattgcaccg actgaatcca tcacgatttg ggaggaaatt ttattggacc
    10201 ttcaagagcg cggtttgaaa aatgtcctcc tgttcatcac ggatggctta aaggggatgg
    10261 taggagcgat tagtcggttc tatcccaaag ctcgttttca acattgttgt gtacacgttt
    10321 cccgtaatat cagtcataaa gtgcgtgtcg atgatcgtaa ggaagtctgt gatgatttta
    10381 aaatggtgta tcaagcctca tctaaagagg tggcgttgga agcacgtggt acttttgcgg
    10441 aaaaatggaa aaccagctat ccaaaagtgg ttgaatcgat tctttcgaac gatcacttgc
    10501 tcactttcta tgatttcccc ttggccatac gcaagagtat ttattctacg aacttgattg
    10561 aatcctttaa taaacaaatc aagaaataca gccaccgcaa ggaacagttc caaaatgaag
    10621 agtcgatgga acgtttctta gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc
    10681 gcagtcataa aggtttccaa caggccgaag gcgagctcga acaaatgcta agccaactga
    10741 ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt attgacgctc
    10801 ccttataatg agaactttta ctaacataat ttatgataaa cttactatat aatcatatta
    10861 attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg aaattttaga
    10921 aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat aggtgagaat
    10981 gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga tagttattac
    11041 tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa tgggtggcaa
    11101 gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga atttgacaat
    11161 gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga agaattgatt
    11221 tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa caaagctatt
    11281 agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa cgaatttaga
    11341 aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa tctaaatttc
    11401 tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat agctagtaat
    11461 cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga tattttcaag
    11521 catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat gaaaaacaat
    11581 cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc agataatgtt
    11641 gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt tcctgaaatt
    11701 aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc agtgtattct
    11761 cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc aaaatttctt
    11821 tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca tctaaatatg
    11881 atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga actacttaat
    11941 attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa aactctgttc
    12001 gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca ttctgtacat
    12061 ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa tggcaaatct
    12121 atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt aaaaaagaac
    12181 aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa tggattgctt
    12241 ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat tcctcaaata
    12301 gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat taatgttggt
    12361 agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca gagaaagtgt
    12421 gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt gtataaaagc
    12481 actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt taatgaaaac
    12541 aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt agatgtgaat
    12601 aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa agataaaaaa
    12661 acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag aaatttagcg
    12721 atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt aggtgaaaaa
    12781 attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt tgcatgtcag
    12841 gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga cttgtacgag
    12901 gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac agctgatttg
    12961 aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt tgtgtatcta
    13021 atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa atgacaaata
    13081 tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg gaaagtgaag
    13141 ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt acaaggaaaa
    13201 ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct atcacataca
    13261 ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata gatgatgata
    13321 ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt tatcctttta
    13381 aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat actcaaagag
    13441 cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat gaactgaaca
    13501 aaattgattt gtcactttct catgatttta aaattaatga agttcagtat actcaagttt
    13561 ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat atgattttac
    13621 tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt gctagtaata
    13681 taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac aatgtaatag
    13741 ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat attgaccaag
    13801 caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa atgctaacta
    13861 gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag agattagtag
    13921 acttctactc taattttcag agttgtcctt acgagattga aagcagaggg aataataaaa
    13981 acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta cccaacataa
    14041 taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat atttgtgtac
    14101 ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa gaattaccgt
    14161 attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat agtttatttt
    14221 tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga agaaagctaa
    14281 ttgcaaacga tattttaatt atattaaaaa atgaatttgg aatagaagtt aaagattatt
    14341 ttattgactt agatttatta cagctaataa acagtgtgaa aagaatgtat agtggagacg
    14401 acaacggtgt tgatttatac aaatttgtta ccaaacatat atttgttaat cttaatattg
    14461 acgagaaaaa ttacccgtct atttttaagc actatctatt ttttattaac gaagtagaag
    14521 atcggataaa aaataattca ttatctagta gtgtatctag ttttaagaag atgtttaaat
    14581 ctcgagatgg tatcacactt actacagcac ataaagttaa aggaaatgag tatgatactg
    14641 ttatcgctat taatcttcta aacgggaaaa ttcctcattg gaatgaaatt tttaatgttt
    14701 ctgataaagg aattagttca gcgagaaaac tactatacgt aatttgttca agagcaaaaa
    14761 ataatttatt tcttttctca gaggtgggtt attttacgag taaaggagac ttgctttatc
    14821 ccactcaatt attagataaa atagaatttg attataatat ttaaaatacc taggtagtcg
    14881 ttgttttaaa tatagtagga atcggacgct cagtacggag cgtctatttt tttacccaat
    14941 tttgaaagga agtgaactta tgaaaataaa aaatcaagaa tcaaaaggtc gctctccccc
    15001 ctttaaaacc atcacacaaa taagattcat tcaaacataa aaacagaaag gataggtaaa
    15061 aatatggaac ttaaatatgt cattcccaat atggaaaaaa catttggaca attagaatat
    15121 gctggtgaag ggaacattga acaacgtcga ataaatggca gaccaaccat tctctctcgt
    15181 agttacaatt tatactcaac gattcaacga gcagatgata ttgttgtcat tcttccctcc
    15241 gaagctggcg agaaacattt tgaatccgac gaaaaagtca aactcatcaa cccacatatt
    15301 accgcagaag gttataaaat tggcaatcga ggatttacca attacattat gcacgcagac
    15361 gatatggtga aagcataaga aaggaaaggt gaaaaaatat gagattagca gaaggtattg
    15421 tgattgaaaa agaccagaca tttggcacat tgaaattttc cgccttgcgt cgtgaggttc
    15481 gtatccagaa tgaagatggt acggtttccg aagaaattaa agaacgtacc tatgacttaa
    15541 aatcaaaagg acaaggacgc atgatacaag tcagtatccc cgcttctgtt cctttgaaag
    15601 aatttgacta caacgcagaa gtggaattaa tcaatcctgt ggcagatacc gtagcgacgg
    15661 ctacttttcg aggggcagac gttgattggt acgtgaaagc agacgatctt gtattaaaag
    15721 gacagacaaa acctcatcag aaatataaaa aagaagcgtc cgaaagtgaa aaataggtgt
    15781 tgcttatgac aaacacacca ttgattttga aagaaattcc gaaggatgaa gccatttctt
    15841 ttattcggca ataccattat tcaaagatac tccctagatt gtgtaaatac tttctaggga
    15901 ttttttcaga agaaaaatta ttaggcgttg tggaattagg ctggggtacg caacccctac
    15961 aaaccatacg caaactcttt cccgacagtt cccttcaaac cacagattat ttagaaattg
    16021 gaaagatgtg ttttcttcca gaaatgaatc aaaccaatta ttttggcagt caagctcttt
    16081 cagctctaat taagtggttg aaagaacata cggactgtca ttttttatat acactggcag
    16141 atggaattga gggaaaatgt ggctatgtct atcaagcgtc caacttcttt tactgcgggt
    16201 atttcaagac aagtgtctat cgtgataagc agtcatggga aaagatacac cctcgaagtg
    16261 ctcgcctttt attagaggaa aatgctcgct ttgaacaggt agagaaaaag cattggcttt
    16321 cccaggcatt ttgcgaatac aaagggattg agaaaatcaa tggacgcatg tttcgctatc
    16381 tctatccgtt gacaaaagaa gcgaaaaaac tgttaggaca cactctctat cgacgtcatt
    16441 attatccgaa agaaaaaaat ttacgctttg aaaagcgaat cgcttatcag aaatatgaag
    16501 ccatttctca accaactttt gataaacagg ctcgtatcta caatacacaa ttattttgat
    16561 aggtggaaag gaggattttt atgccattgc ttcatgacag aggagagcga atcaaagcca
    16621 acgataagca cttggtctat cattttgtac taatttcatt gctggtagtt tttctagtcg
    16681 tcctactccc ttttcattgg agagcgttac aatcggtgaa ctggcaagca gttcagttag
    16741 atacattttt ctatcaactt catacgccat acttttggag cagtattctc actgctcttt
    16801 ttgtatgcgg acttatcagc tggctttatt atcactatcg tatagattct ataaaacagt
    16861 tagagcaccg tcaaaagtta gctcgtatga ttttagaaaa tcattggtat gaagccgaag
    16921 aaactcaatc agagggattc tttaaagatt tacccagcaa gaaaaccaaa caacgaatcc
    16981 gccattttcc taaaatatat tatcgaatga aagatggact aatccatatt catgtagaaa
    17041 tcaccttagg gaaatatcaa gatcagcttt tgcacctaga aaacaaacta gaaacaggat
    17101 tgtattgtga attagtggaa aaaacgttga aagattctta tgtagagtat gtcctacttt
    17161 atgatactat tggcaatcga attggcattg aagacgtggt agctcaagat ggacgtgtca
    17221 aattgatgga atcggtctat tgggagttcg attctctccc tcacatgtta attgctgggg
    17281 gcactggtag tggaaaatcc tattttattc tcacattgat tgaatctctc ctgcatacaa
    17341 atgcaaagct ttatattctt gaccctaaaa atgctgattt ggcagactta ggggcggtca
    17401 tgaataatgt ctattaccgt aaagaagata tgttggcatg tatcaatcaa ttttacgaag
    17461 atatgattga tcgcagtgaa accatgaaac aacaccctaa ctataaaaca ggagaaaatt
    17521 atgcttatct tggtttgtct gccaattttc ttatttttga tgaatatgtg gcaatgatgg
    17581 atatgctagg tcgtgaaagt accaccgtta ttcataaact caaacaaatt gtaatgttag
    17641 gacgacaagc aggcttcttt ttaattcttg cctgtcaacg tccagacgca aaatatttta
    17701 gtgatgggat tcgggacaac tttaacttcc gtgtggcttt gggtcgtatg tctgaaatgg
    17761 gctttggtat gatgttcgga agcgatacac aaaaacaatt tttcttaaaa cccatcaagg
    17821 gaagaggtta cgtggataca ggaaaaagtg tgattagtga gttttataca ccccttgtac
    17881 caaaaaggta cgatttctta ggagaaatag gaaaagtcat tcaaaaaaag caatctgagc
    17941 ctgtaccgca cgaagtgaaa ggtacaggtt cagacttgct ttgattggtg tggcgttagc
    18001 cacgccaatc ctttagcccc ctctatctaa caggggggca caaatacaac tgaaaacagg
    18061 caattctcat caaccaatgt tgatggtaca aaggattgcg gaaaatatga aaggtgtaaa
    18121 ctcaagggtc atagtttaca tcttttttag attggaggtt tgcgaatgga aaacgaaaaa
    18181 atttggatta aggaattgaa agaaaagcga ctggcttatg gcgtttctca aaataaactg
    18241 gcggttgctt ctcatattac cagaccttac ctatcggata ttgaaacagg caaggcagtc
    18301 cctactcaac aggtgaaaga agatttactg aacgctttgg aacgcttcaa tcccgataac
    18361 ccattggaga tgttattcga ctatgtacgg attcgattcc ctaccaatga tgtggctcaa
    18421 attatcggtg aggttttacg tttgaatatg gattatatgc tacacgaaga ctttggctat
    18481 tattcttatc ccgaacacta tcgtttcggt gatattgttg tcctagtctc tcatgatgta
    18541 agtaaaggtg ttttattaga actgaaagga aaaggctgtc ggcaatttga aaactttcta
    18601 ttggctcaac atcggagctg gtatgatttc tttcaagatt gcatggaaca taaaggtatt
    18661 ttcaaacgct tggatttagc tatcaacgat aaaacaggga ttttgaatat tccagatttg
    18721 gcaaagaaat gtaaacagga agaatgcatt tccgtctttc gaagctttaa aaattatcgc
    18781 agtggtgaat tggttcaccg tgatgaaaaa ccatacatgg gaaacacact ttatattggc
    18841 tcattaaaaa gtgaagtcta tttctgtata tatgagaaag attatgagca atacgtgaaa
    18901 aatgatgttc ccttagaaga cgcagaagta aagaatcggt ttgaaattcg gttaaaaaat
    18961 gaccgagcca cccaagccat gaaagattta ttggcacatc aacaggcgga aaaaacagcc
    19021 tttgaaatta tcaatcgcta tatccgcttt gcagataaag acgacactaa acgtcggagt
    19081 gattggaaaa ctaatgaacg ttgggaatgg tttatcggta aaaatcgtgg agcattacgc
    19141 ctaaccacac agccagagcc ttattctttt gaacgaacat tgaactggtt acatcatcaa
    19201 gtcgccccta cattaaaaat cgcaagcatt ttagatgttc tcaacggtac aaccattatc
    19261 tccaccatga ttcaggaagc caaactgaca gaaaaacatg agaaattaat tgaacaacaa
    19321 catctagcca tggaggattt gattacatga gccttttaac aaaagaatta aagaagttag
    19381 ggtttcagtg tggcattgag tttcaagcgt atatacaaaa tacagggaaa tatacgtctc
    19441 tcattattga gggaaaacgg caagctggtg ataccatcta tacctatgat ttttacaagg
    19501 tgagatttta taataattat accaatcgag taaccgtcta tggagagcat ttaacccctt
    19561 ttcaattatt aaggcgtgta aaaagctata tctattatcg agaaaaatat cttaaggaaa
    19621 ggaggacaat cacatgaatt ttgggcagaa tttgtacaac tggttcttgg ataatgcaca
    19681 gtcattggta ttgttagcga ttgttgtcat tggactgttt ttaggtttta aacgagaatt
    19741 ttcaaaactc attggcttct tgattattgc tctaatcgcc gtcggacttg tgttcaatgc
    19801 agctggcgtg aaagatgtgt tattaaactt atttaaccga atcattggtg cttaacagat
    19861 atttttcatg ataaaatagg aagtatatat tttgttatga aaggatttat gaatatgttt
    19921 acaaatgatc aatttaaatc tcttttaatt aaaacattag aaagaaagag ctcttcaaat
    19981 tattatgaag gaggtaacga gattgtttct aaaatgaaaa tatcagaggt aactttagat
    20041 gagactgacg attttactta ttataaagga tataaaaagg gctggaatac gctttgcaca
    20101 tatcttaaaa taagagttcc ttttgatgat ttagactttt ttgaaagtca taaagatttg
    20161 attacccaga cagcaagctc catatatgat aaacaaggtg ataatgtatt agtggatact
    20221 attttagtac cacttccaga gaattacgaa gtaattaact ttagtcagtt gaaaattagt
    20281 gatgtggttt cacaagctat tgaagacgct gagtctttta tgagtaatgg tgaatatcaa
    20341 cgtgcctttg acagagttca cactgctttt catggatatt taattgaaat actaaaaaaa
    20401 tatgaaatta cagttcctag agacgaaaat ctatctaaat tatatagtag gatacaacaa
    20461 ttaattgaaa aagaaattca acctactgaa cttgctgata tagtgaagac aacgataagg
    20521 tcatcaaatg gaatgattag ctctcttaat gaagcaagaa ataggcattc acttgcacac
    20581 cctaatacaa atattattgg aaagagagaa gcaaaactaa taataggtat ttcaagtaca
    20641 gttacagatt atattagcgg ataccttgat aagtagcgtc attaactgat tgctactttt
    20701 tttatgacta tttttataaa aaggatgtga taagaattgg aaatgaaagt ctatgtggca
    20761 aatttaggac ggtataatga aggtgaatta gtcggagcat ggtttactcc accaattgat
    20821 gaggaagaaa tggcagaaag aatcggttta aatgaggact acgaagaata tgcaatacat
    20881 gattttgagc ttccttttga tgtggacgaa tacacgccga ttagtgaaat caaccgtcta
    20941 tgtgaagcca ttcaagaaat cgaaggtaca cctatttata atgaactcaa agaaattcaa
    21001 ggcatgtggt ttagtagctt ggaagaatta ctggaaaaca aagaggatat tcattgttat
    21061 agtgattgtg attcgatgga agatgtcgcc cgttattacg tggaagaaac aggacagctt
    21121 ggtgaagtcc cttccaattt acaaaattat attgactacc aatctttggg acgtgatatg
    21181 gaaatagagg gtaattacct agttacctct catggggtat ttgaatattg tcagtaagtt
    21241 taacaatccg tgttataatt aaattaataa atagcacgga gaggtataaa ttatggtaaa
    21301 tgtatttgaa aatcttaaac tattaaaact agacatgaaa gataaaggct gggtgataga
    21361 ttccttttac tttcgttaca aacagcaaaa ttatattgta cttgttaagc tatttgaaaa
    21421 agaggaaaaa gttccagaat atgctctgtt aaaactagaa tttttaaagg aaaacgattt
    21481 ttcagatatg ctagctgtct atgctaattc ggtaaaatta tatacagaca cgaaaacaat
    21541 aagagaatat tttggtatcg aatacagtag taacttggga gatgttttat ttcaatttag
    21601 ccaaactctt gctcgattta ttccgacaga agttagtgag aagaaaaatg aagaccaaaa
    21661 agaagccatg tgtttctcat taagtcaaag tgattcagaa gatcccagaa aaaaatattg
    21721 tttttcagtt agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac gaagtccttt
    21781 taatgacaat aaaacgagat tgtatagacc cagcttatat gaacgattag ggtcagatac
    21841 caatttaagt ttcagatatt caatgaatcc agatgatgaa gaaactgatg aaggtattat
    21901 tgcaaaatgg acaaaaaata aaattgaata gttaaagcat tgaaagagca atcacctatt
    21961 ttgattgttc tttttctatt tttacggaaa ggacgtgaag acatgaaaaa aataagaagt
    22021 tacaccagta tttggtcagt agaaaaagtg atttatgcga ttaatgattt acaactccct
    22081 ttcccaatta cctttacgca aatgacatgg tttatcgttt ctttgtttgt cgtcatactc
    22141 tttggcagtt taccaccact ttctctgatt gacggagcac tactcaaata cttagggatt
    22201 cccattgttc tcactgggtt catgtgccaa aagacctttg acggaaagaa accctatgga
    22261 tttctaaagt ctatcattac ctatgctcta cgccctaaag taacctacgc tggcaaacaa
    22321 atacgattaa aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag tgaacaacta
    22381 gatggcatat ccgattaaat atatggaaga taatctcgta tttaatcatg atggcgaagt
    22441 attcgcttat tatgaattaa ttccctataa ttacagtttt ttaagttccg atgaaaaaat
    22501 tcaagttcat gataatttct gtcaattgat tgcccaaaat cgtgatggca agattcatgc
    22561 tctgcaacta gcaaccgaat caagtattcg agccacacag gaacaatcga aaaaagaaat
    22621 cactggacga ttaaaagaga tagcttacga aaaaatagat gaacagaccg aagcgttaat
    22681 ttctatgatt ggtgacaatc aagtagacta tcgctttttt attggtttta aactctacct
    22741 caatgaacag gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag catttgcgga
    22801 attctttcat ggtgtgaatc acaaattaat gggcgatttt gtttctatgc ctatggcgga
    22861 aatcgaacgc ttttctaaga tggaacagtt gttagaaagt aaattatcca gacgctttca
    22921 gattcgtaaa ttggagaaag atgattttgg ttatattatt gaacatcttt acgggcaaac
    22981 aggtgtccct tttgatgact acgactacca tttaccgaaa cgatacgaag ataaaaaagt
    23041 gttggtaaag aaatacgata ttctcaaacc cactcgctgt ttaatcgaag aaaatcaacg
    23101 ctatttacgg attgaacagg aagataccac tacttatgtg gcgtatttta caattaatag
    23161 tattgttggt gaattggatt tccctaatag tgaaattttt tattaccagc aacagcagtt
    23221 tactttcccc gttgatacca gtatgaatgt tgaaattgtc gccaataaaa aagccttatc
    23281 taccgttcgc aataaaaaga aagaactgaa agatttagat agccacgcat gggaaagcga
    23341 caacgaaacc agtactaatg tggtggacgc tttagaatct gtcgatgaat tagaaagtaa
    23401 tttagaccaa accaaagaat ctatgtacaa actaagctat gtggtacggg tatcagcaaa
    23461 gaatttggac gagctgaaac aacgttgtga tgaagtaaaa gatttttatg atgatctgtc
    23521 tatcaaatta gtacgacctt ttggcgatat gctaggcttg cataatgaat ttattccctc
    23581 aagcaagcga tatatcaatg actatattca atatgtcacg tcggattttt tggctggtct
    23641 tggtttcggt gctacacaaa tgattggtga aaattctggt atttacgtcg gttacaatct
    23701 cgatacaggc agaaatattt atctccaacc tagccttgca agtcaaggaa ttaaaggctc
    23761 tgtaacaaat gcattagcgt ccgcttttat tggttcatta ggtggcggaa aatcattctc
    23821 taacaatatg ctggtctatt attcagtact ctttggcgga caggcaacca ttgttgaccc
    23881 aaaagctgaa cgtggtgaat ggaaagaaac cttacctgat attgcccatg aaatcaatat
    23941 tgttaatctc acgagtgaga acgacaataa agggttactt gacccttatg tgattttaaa
    24001 actcccgaaa gattcagaaa gccttgccat tgatattctc acgttcttaa caggtatttc
    24061 cagccgagat agcgagaagt ttcctgtcct gcgtaaagct attcggagtg taactcaaag
    24121 caaacaacga gggttgttat tggtgattga tgaattgcga aaagaagata cgcccatcag
    24181 caatagtatt gctgaccata tcgaaagctt tgtcgattat gattttgccc atctgttatt
    24241 cagtgatggc agtgtcaccc aatccattag cttggaaaaa caactcaata ttattcaagt
    24301 ggcagatttg gtcttacccg attccaatag ctcttttgaa gaatacacta ccatggaatt
    24361 attaagtgtg gcaatgctga ttgtcattag tacctttgct ttagacttta tccactctga
    24421 ccgttccatt tttaaaattg ttgaccttga tgaggcttgg agttttttac aagtggcaca
    24481 aggaaaagct ctatctatgc gattggttcg agcaggacgt gccatgaatg caggggtcta
    24541 tttcgttact caaaacgcgg acgatttact agatgaaaag atgaagaata atattggctt
    24601 gaaatttgcc tttcgttcca ctgacctagt ggaaatcaaa aagaccttag aattttttgg
    24661 tgtggacaag gaagatgaaa acaatcagaa acgcttacgt gaattagaaa atggacaatg
    24721 tttagtttca gacatctatg gtcgtgtagg ggttatgcag tttcacccga tttttgaaga
    24781 gttgcttcat gcctttgata ccagaccgcc tgtgcgaaat gaggtggaag aatgacacaa
    24841 aaacaaaaga aatttcttca ctatagttgg ataacgctcc tttgtgtggg cgttatttta
    24901 cttttattag gcacgctggg caatgcagta caagcgacag gcttagttga tgaaaccatt
    24961 gatacaagca atgaatactc caagtatggt ttgaatcaat atcagcttga ttattatgtg
    25021 gacaatagct ggggctggct tccatggaat tggagtgatg gcataggaca atcggtgatg
    25081 tatggattgt atgccattac taattttatt tggacaattt ctctttatct atcgaacgcc
    25141 acaggttatt tggtgaaaga agcctatgcc ttagatttca ttagtcaaac atcccaagca
    25201 attggcgaaa acatgcagac gattgcaggc attacaccga atggcttttc cagttcaggg
    25261 ttttatgtcg gtttcctcct tctttttatt ctcattgttg gaatctatgt tgcctataca
    25321 ggattggtta agcgagaaac gacgaaagtg atacgagctg ttttaaactt tgtcgtggtg
    25381 tttattctat cggcttcctt tattgcgtat gcacccgatt atattgcgaa aatcaatgat
    25441 ttttccaaag atgtcagtac cgccagtctg aatattggga caaaaattgt tcttcctaac
    25501 acggatagtc aagggaaaga cagtgtggac atgatacgtg acagcttatt ctctgttcaa
    25561 gtaaagcaac catggctatt gcttcaatat ggctccactg atgtagaagc gttaggggaa
    25621 gaacgtgtgg aaagtttatt gtctaccagt ccagatacaa acaatggtga agatagagaa
    25681 aatgtagtga ttgaagaaat tgaagatcat gacaatacct atttaaccct accgaagacg
    25741 atttccagat taggaacagt cttctttttg tttgtattca atattggtat ctcgattttt
    25801 gttttccttc taacaggaat catgattttt tcgcaagtgc tatttattat tttcgctatg
    25861 tttttgccaa tcagtttcct attaagtatg ttgccgagtt ttgatggcat gggcaggcat
    25921 gccattacca agttatttaa tgtgattatg acaagggctg gtattacctt gattattacc
    25981 gttgccttta gtatttccac tatgctttat tcgctgtcag caagttctcc ctttttcatg
    26041 attatgtttt tacaaattgt gacctttgca gggatttatt ttaaactggg cgatttaatg
    26101 agcctattca ctttacaaag caacgactct caaaacatgg gcagacaagt gtttcgcaga
    26161 cctcgtcaaa tgatgaatcg acaaaccaga agatttcaac gcagtatgag taagattttt
    26221 cgtggaaaag cagggtcaac gaagcaaaag acaatgacaa caaagcagaa tcaaaccaat
    26281 aaagctaacc atactcgtca aaatgaacgg actacaccaa agaaagaaac agcactttcc
    26341 aatgtgaaaa agcaacaagg cggtatcggg caacgattag gtgaaaagac agcccatgta
    26401 ttggacacga aaaatcgtct agtcaataaa agaaagcagg caaaacaaca gatacaggcg
    26461 acaccaaccc atgtcaaata tgcccttcat caaggaaaag agaatgtcac tcataatgta
    26521 acagacttta aacaatctat ggcagatacc aaagctcaac gcaaacaaca gcgtatgaac
    26581 cagcaggagc aaaaacgaaa gaacattgct caaaagcgtt tggagctgga taaagcaaaa
    26641 gaaaaaagac gaaaagaaaa cccatcatct cctgttcaga ataaccagca gcatgttcgc
    26701 aagcgtcagc aaaccgtcac gcctgttttg tcacaaaaca ctaaacgtga aaatcatcag
    26761 cgaccattga aaaaagagca aaaacaaaag acaccaccga agcaattcaa tcagaggaag
    26821 ctaaataaaa gaacggataa gcgaggacaa caaacatgaa gctaaaaata acagtgattt
    26881 gtagtgtggt cttctctctc ttcttttttc tcttattatt cctagtcatt ttctttgcag
    26941 atgatacgga cagtggcgaa aacaacaagg attcttctat tccacaaggc ggtgtgaccg
    27001 tttcaccaga agtgttggca caccgtccgt tgattgaaaa atatggtaag gaatatggga
    27061 ttgaagacta tgtttcttat atcttagcca ttatgcaagt ggaatcgggc ggtacagcag
    27121 aagatgttat gcaaagttcc gaatctttgg gtttaccgcc caacagtttg agtaccgaag
    27181 agtccatcaa acaaggggtt aaatatttca gtgaattatt aaccagtgca gaacaacaag
    27241 gagtggatat agattctgtt atccaatctt ataattatgg cggtggcttt ttaaattatg
    27301 tgaggagtca tgggaaaaaa tatacctatg aattagccga acagttttct aaagagaagt
    27361 cgggtggtca aaaagcagac tatcccaatc ctatagccat acctgtgaat ggtggttggc
    27421 gatataacta tggaaatcag ttttatgttc agttggtttc acaataccta accgacactt
    27481 ccccaacaga atttgatgat gaaaccgtgc aagtcattat ggacgaagcc ttgaaatacg
    27541 aagggtttcc ctatgtcttt ggaggggcgt cgcctaccac ctcttttgat tgtagtggct
    27601 taatacaatg ggtgtatgac aaagctggca tttctctccc tcgtgtcgcc caagatcagt
    27661 atgacgctac gcaagaaatc tctatggaag aagcccaagc aggcgacctt atcttctttc
    27721 attcaaccta taatgcaggc acatatgtga ctcatgtggc gatatattta gagggaaatc
    27781 gcttttatca tgcaggcgac ccaatcggtt atggcgactt aagcagtcgt tattggcaag
    27841 accatctgat tggggctaga cgtgttattc ataactaaga aaggaatgaa tcaaatgatt
    27901 ataaaaatcg agcgaaaaga aaagaaggag aaaccaaaga aatctaaaaa accgagcaag
    27961 caaagaaaag ttccgatgat taaagttggc acgcataaga aattaacctt tgttttatgg
    28021 gttttattga ttggtagtgt cggatttgga atctataaaa attttactgc cattgacact
    28081 catactgtcc gtgaaacaga aatcattaaa cagcagattg tcgataccaa ccaagtagaa
    28141 agttttgtga aatcctttgc gaaagactac ttttcgtggc aacaatccca agaagccatt
    28201 gataaacgaa atgaaaaatt gacccattat ttgacggaag aattgcaggt attaaatgaa
    28261 gaaatgattc gtaaggatat tcctactagt tctagtgtga acgatataca ggtgtggcag
    28321 gtttctcaag tgaatgagaa cacctttgag gtactgtttt ctgttgaaca agttattacc
    28381 gaagacaaag acaaggaaac tatatcgtct agctttcatg tggttgtcca tatagatgaa
    28441 tcagataata tggttattat caaaaatccg acaatgagta agaaacctca aaaatcggac
    28501 taccaaccca aacagctaga aagtgaccat acagtagata cagaaacgat ggacgaaatc
    28561 actagctttt tagaaacgtt cttccagctc tatcctaccg ccacagaaaa agaattgact
    28621 tactatgtaa gtaatcatgt gttacctatg attaacaagg aatatgtttt cgaggaattg
    28681 gtgaatccta tctttaccag aaaagataac caagtaatcg tgaatgtcgc tgtgaaatat
    28741 ttagatcaag aaacaaaggc aacccaaatc tcgcaatttg agttgatatt agaaaaacaa
    28801 gataattgga agattgtaaa ataacaaata ttggtacatg attacagata ctttgtaatc
    28861 atgtactctt tttgataaaa aattggagat tcctttacaa atatgctctt acgtgctatt
    28921 atttaagtga ctatttaaaa ggagttaata aatatgcggc aaggtattct taaataaact
    28981 gtcaatttga tagcgggaac aaataattag atgtcctttt ttaggagggc ttagtttttt
    29041 gtacccagtt taagaatacc tttatcatgt gattctaaag tatccagaga atatctgtat
    29101 gctttgtata cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga
    29161 tttttatgcc cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg
    29221 agttttagct catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag
    29281 tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct
    29341 tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa
    29401 tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg
    29461 ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc
    29521 acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat
    29581 caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa
    29641 actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac
    29701 gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga
    29761 gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag
    29821 atttcagaat tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat
    29881 tgataacctt atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc
    29941 tgaactttgc ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata
    30001 ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta aacctaaatg agtatcaaat
    30061 tgcaactgaa tttgatactc gttttttatt gttaaatcaa cgtttgttgt taatttacac
    30121 cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt gaaataatta ttgtaggaat
    30181 gcaaattggt gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc attttcctat
    30241 gcaatttctt gagccaaaaa ataagaacgc taagtctgtg gattgggaaa tatcagagca
    30301 agtaagggtg atagttaagc aatacgctga atatgcagag cgtactgaaa gcgaagcagt
    30361 ggatgaattc ttattgaaca ttttagacga taagaagttt attgaatgga ttgccaacaa
    30421 gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg gttgatagac
    30481 aatgaccaag cttaaaagat tagccacgaa ggaagatgaa attgtagatg ttaaaattcc
    30541 gatttcaaac gaagaattaa aagatagggc taaacaatat gatttattaa ctccaaaacg
    30601 atttgcaaca aggtataaca aaatgttgtt cttgccaacg tcttttaaat ggaacggttc
    30661 tgaatatccc attcaatata attactgcat caatccgttt tgttgtaatt ttgggaaaga
    30721 acagcacaaa ttcaaagatg taaaagggaa accaagtcga tataaaatga ctggtagttc
    30781 caaagataaa ggtcataaag gtatgtactg caatgacaat cctattggca gaggtgtatc
    30841 gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa ttaaacgcct
    30901 tatagaaatc aatagcatcc aagatgttga gccagattat caatttcata aagaaggctg
    30961 ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa gggggaaaag
    31021 taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta atgttctacc
    31081 aaaaagagaa gaaaccacta cttatcacca acaaaagaac accattcttc caatgttcgc
    31141 taagatggtt gttggaaggg tttcagtcag tcgtacttgt gatattttag gaattggagt
    31201 tggaacctac tatcataagc tggagtggct gtataggcgt tgtttggagt ttttagaacg
    31261 atatgagaca caaccccttc aaacaaagaa atttaacgag atgtggctta atacggacaa
    31321 gatgcactac tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga aatatacggg
    31381 gtttgaagac cttaatatgc aaacgtatat agttgtatct gctgaggtct tatcaagata
    31441 tgtgttccgt tctgatgttg cctatgattg gaatatttca atggatgaat taaatgaaga
    31501 tacaagaaaa ttcaaagaag accatctcaa tacttttagt cgtaaaaatg ataggttaga
    31561 ttggtcttat tatccacaag aaccatccgc taacgatagc gaaaatagga atgcttatct
    31621 tcacgaatta gggaaaatta ctaataggag tagatttgtc gatggtttaa atgttgatgc
    31681 tccttatact acaactgctc actattggtt aattaaacaa atggttaatg ccgacgaatg
    31741 gcgaatgatt agtgatgatg acttctctat tagaaacgcc ttttatagag tattcacgaa
    31801 agaattaagg ttgagcgatg cacatcattt tatttgccaa gttaacaaga cgaaaagcag
    31861 aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt ggggagatat
    31921 cagaggattc aaaacaaagt ttctacggac tttggcatct cattatctaa ctgaattact
    31981 aacaagtcat caattccacg aagaggctat tagtaaagat ggagaaagat atcgcaaata
    32041 tgccgataac cctatcaaac atccattagc cactaaggat aaaggatttt attcagttga
    32101 ttgtagaacg gatttatctg ctctggaacc aaacgaaatc gcaaagatgc tattaaatgt
    32161 taatgaccat tcgaccaata gctttattca acagataaga cgttatatat catctcttga
    32221 aagaccatta acaacagctc gtggtgataa gaagagctat atttacgcta acttcaatcc
    32281 gaagtatgct caatttgcga ttacaattct aagaacttac tacaactttt gtcgcccttt
    32341 taaatcagcg gataaaaagg ttttgacacc tgctcaaaga ttagggatta cagataaaca
    32401 atttgattgg aaagatataa tttatttcaa atgacaaaaa gagcttggag taatcccaag
    32461 ctcttgtgct ttatctttac tgttcttctt caattaacgc acccgttaac tcaataagtt
    32521 ttttctttca tatacatgaa aatagtagtt atacggattt ttatcattct ttatcccttt
    32581 ttgggcaaat acctcattcc attcctcata attcacttct ggaaaaaaag tatctccttc
    32641 gaattcatga tgtatttttg tgatgtacat tttctcaaca taagggaaaa acaaattata
    32701 aatctgttct cctccgaaaa taaaaatttc ttcttcgttt ttacataact caaaaacatc
    32761 ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat
    32821 aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat
    32881 tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg
    32941 aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt
    33001 catttataac acagcctttt taactaattt accatctaat ttcttatttc tatcaaaaaa
    33061 ccttttctca ttatcctgcc cgttagttga ataagcaatt ttttctataa gttatattat
    33121 cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc attttataaa
    33181 tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta
    33241 cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag
    33301 tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac
    33361 agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga
    33421 aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact
    33481 attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc
    33541 gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga
    33601 tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg
    33661 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
    33721 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
    33781 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
    33841 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
    33901 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
    33961 gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc ctgttagtac
    34021 tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta gaaaagctgg
    34081 aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg aatatctttc
    34141 acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa
    34201 taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt atcgcaatga
    34261 tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag gatatcaggt
    34321 taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag ataaagtaag
    34381 atatatgttc aataaaataa cttagtgcgt tttatgttgt tatataaata tggtttctta
    34441 ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag gaggagattg
    34501 ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca agactacgaa
    34561 tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa
    34621 ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac gccaagacgc
    34681 agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt ttatctgaat
    34741 tttactgact agccttcaat atttcttgct tcattgatac cttttgctag tgcttccatt
    34801 aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa ccttcgagtg
    34861 cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg gagtaatgat
    34921 acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat atttgttaaa
    34981 taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc ccgtttcatt
    35041 cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc ttttttacgc
    35101 atactaaatc acctctatac attttactgt tcctatataa ccataaacag gtatagaaaa
    35161 acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa tgcgatgaat
    35221 ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat aaaatttagt
    35281 catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc gccatgagaa
    35341 agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt atccacctgt
    35401 actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa aaatcaaagc
    35461 atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata aatatctttt
    35521 aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta gtatgaaggg
    35581 agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa gcatgaaacc
    35641 atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac aagttattga
    35701 agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag aagtctcgtt
    35761 ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt acgtcactga
    35821 tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt tattaagtga
    35881 agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt actttatgga
    35941 tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg tttatcgtca
    36001 tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag aatgaaaaca
    36061 gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga cgtggaagca
    36121 attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt acgtcgcatg
    36181 acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg cagaatggaa
    36241 acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt tttcgtggaa
    36301 gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca tcaaagcata
    36361 ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac aaccaaataa
    36421 aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc atgacctaca
    36481 aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga ttgctgccat
    36541 gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt tagaagcgtc
    36601 acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg tgagctttgc
    36661 tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat aaaactattt
    36721 gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa ggagtaataa
    36781 tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct ttttcagcag
    36841 acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac tatcaatctc
    36901 gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt cggttttcaa
    36961 atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg ttaaagaccc
    37021 aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt atgattctaa
    37081 agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag caagagaaga
    37141 atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg ataaaatgac
    37201 gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg taaagctcag
    37261 aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg ataaaatggc
    37321 tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat caaagaagtg
    37381 taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg atcgagcaat
    37441 tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga aaaagcaaaa
    37501 gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc taatttacaa
    37561 agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta tgactggaat
    37621 gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact caggagtact
    37681 aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg ttgaaccaaa
    37741 aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt atttatccga
    37801 atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct ataacggtat
    37861 tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc tggctggtat
    37921 ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa tcagtatggg
    37981 agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga cgactttagg
    38041 aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat taaatggagc
    38101 catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc aatttactgt
    38161 agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta aaaagataag
    38221 aagagccaga aacccttggt ataagaggat ttctagctct cctacaacta ttcccactcc
    38281 actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg ggctacttcg
    38341 tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc aaagtcagcg
    38401 gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta agtacgtccg
    38461 tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg ccagatatca
    38521 cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc gcgtacgatt
    38581 tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg accagggaat
    38641 ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc acggacttcg
    38701 tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc tggcagtcca
    38761 cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc gataacgtca
    38821 gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag ttttgttgct
    38881 tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg ttctgggtca
    38941 gatacacctt tcaacttgtc taagaagcga tctcttgcat cgactttgat gatgttcaag
    39001 ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg tagcagaccg
    39061 tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag aaccccaacg
    39121 acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc aacttgttca
    39181 cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc ttcacagtga
    39241 caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg acgtacttct
    39301 gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc aatcggacaa
    39361 tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg
    39421 ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg atcgcttgtt
    39481 acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga accgccaagt
    39541 ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc taaattgaag
    39601 atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc gccagaaaga
    39661 atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg gctcaataat
    39721 tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata ctgactaccg
    39781 tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt caaatttatt
    39841 acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat gctaatattt
    39901 tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta tatcggctct
    39961 tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt tccatacatc
    40021 ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat aataattctc
    40081 tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc atttttcgat
    40141 cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga cgtagatttg
    40201 ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat caggctgctg
    40261 gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa caggtacttt
    40321 tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct ccatatcata
    40381 actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac gatttggata
    40441 aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat
    40501 cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg cgatcccgat
    40561 aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca agaacaaaga
    40621 ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga tatctcgaac
    40681 atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat cttgtgtcaa
    40741 aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac ggtagtagtt
    40801 cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat tttaacacaa
    40861 aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata atctttataa
    40921 tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg atagactgtt
    40981 gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc tgaacgccca
    41041 gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac ctttcaattt
    41101 gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg tgtcctgatt
    41161 gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt gggatgcggg
    41221 tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga aatggtcgac
    41281 attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat
    41341 gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta tgcagctatc
    41401 gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt gacaggcgca
    41461 tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa acaaggagcg
    41521 ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat
    41581 aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata gtggagcgac
    41641 gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt tgttgagaag
    41701 tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt
    41761 tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa gtcggtagat
    41821 aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt cttgggggtt
    41881 ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc tgaccatcag
    41941 agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt tgaataacaa
    42001 aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga atgaacatca
    42061 ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga ccatcggaat
    42121 gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg tagagagcta
    42181 tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc aagaaaatga
    42241 gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc tggccacacc
    42301 aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa tgccaatcat
    42361 tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa aggaatatga
    42421 gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga tcggcatgct
    42481 gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc gtcatgcgct
    42541 gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt tgactgtcca
    42601 aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg ctattttttg
    42661 cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat
    42721 cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat acatgattcc
    42781 gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg cacgtttcct
    42841 gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt
    42901 aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta tattgcttat
    42961 gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca gtttactaaa
    43021 gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga ggatcaggca
    43081 atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa
    43141 atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca agtcagtgca
    43201 ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa cttaacattg
    43261 gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat
    43321 aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc cctagtagct
    43381 agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac aggtggtaca
    43441 ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga agaatttgta
    43501 cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt ggcaccggcg
    43561 gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct tccgctgatt
    43621 gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg
    43681 accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct ggcaaagacc
    43741 atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc agatatgtca
    43801 gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat cgaaacatta
    43861 caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag ccaaatggtt
    43921 gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca agaagctctg
    43981 gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca
    44041 gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga gcttcctgct
    44101 aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg
    44161 ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg attgaaactg
    44221 cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac
    44281 gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat tggtgaatcg
    44341 gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa agcggacttt
    44401 accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga ccatactatt
    44461 ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac
    44521 catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga acaattacaa
    44581 ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa cacacccgag
    44641 acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt cgatatggtc
    44701 atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga tatcgaagca
    44761 gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta
    44821 gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc
    44881 aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt
    44941 gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa
    45001 gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg agtagcaatc
    45061 gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc atggcagaac
    45121 tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat gttccttatt
    45181 cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat caaggcatca
    45241 acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct tttttcaaag
    45301 cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac acccccgatc
    45361 ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc cctgatatgc
    45421 cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc
    45481 cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct gactgttgaa
    45541 aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat tatttggttt
    45601 aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca acggctctac
    45661 tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga tcacagacac
    45721 aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag aaaaatgggg
    45781 gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat cttcaaatga
    45841 atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa cgatttttgg
    45901 tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata caggtacgaa
    45961 ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg caactttcaa
    46021 agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt
    46081 agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg ctggattcaa
    46141 agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata
    46201 tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta tgtatgcaca
    46261 aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat tccaagaagc
    46321 aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg accgcgacca
    46381 atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat tgacatctac
    46441 actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg acggtaaatt
    46501 ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc taacagaagg
    46561 acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg
    46621 tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata gccttgttag
    46681 acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt atccaagctg
    46741 ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt caaaagaaga
    46801 acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg ttaggatgtg
    46861 aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc aaagaatttg
    46921 gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt
    46981 tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc ggattattag
    47041 agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca
    47101 aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt gatgccttta
    47161 ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg cttgaccata
    47221 aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca gtcaacccag
    47281 atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac
    47341 tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg acccctcaag
    47401 aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag tcgatcattt
    47461 tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca gttatccgcc
    47521 gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa ttagccgata
    47581 tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag
    47641 tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa
    47701 acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc gatggaaacg
    47761 ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc ggacatatca
    47821 agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa catggtgtag
    47881 gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca ttatctgaaa
    47941 atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg ctgaattatt
    48001 cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga acaacgaatg
    48061 aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg
    48121 aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag
    48181 gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt
    48241 tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt gctgttatcc
    48301 atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat gaattaggtc
    48361 tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag ggggctggcg
    48421 cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact ttcagttgtt
    48481 ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc catccaaggt
    48541 tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga aacgttaagg
    48601 caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc agctgggttt
    48661 ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca
    48721 cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc
    48781 ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt tggaacaagt
    48841 gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac atttgcttta
    48901 catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat gatcagtcaa
    48961 aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg cgtaaactca
    49021 ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact
    49081 ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta tgcaggaatg
    49141 agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc agggcttggc
    49201 ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac ttcgctatct
    49261 attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat cggtattctt
    49321 ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca gacaggagca
    49381 ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt tgtcgggatc
    49441 aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca agaagagatc
    49501 gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga actataatgt
    49561 cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca acaccgctaa
    49621 ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac gtagggctag
    49681 agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg atgagtccat
    49741 cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta ggtatcctat
    49801 tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt atcagcggga
    49861 cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca tggtatggta
    49921 aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca ggattagcag
    49981 atattccggt cattggacca attttcttta aaaatacatt cttacctgct tattgtgcta
    50041 ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc
    50101 gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta tatgctatgc
    50161 gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct gtttttgctc
    50221 agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc atctccatgg
    50281 ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca ttgttctttg
    50341 gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct tcgattccgc
    50401 cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt gttttcttag
    50461 ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa taatctttta
    50521 gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt gttttaaagg
    50581 caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc tgatcgtcat
    50641 ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg ttggttctga
    50701 tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag agcagcttgg
    50761 tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc
    50821 gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact gggaaatcat
    50881 gggattgaat atcaaaactc catttcgtgt gtttccgaac ggattccctg atgatctgat
    50941 caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt atagcggaac
    51001 agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac
    51061 ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt tagaagaatt
    51121 atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata tgattggccg
    51181 aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa gcaatcgcca
    51241 tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag aagcaggaaa
    51301 ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa tcactgaatc
    51361 tgtccgcacg aaaagcaata tggatggcgt tgaccagcta ctgaaagtta tgaaaaacga
    51421 tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg gtcatcgtcg
    51481 ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc cagaattgct
    51541 tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa acgatccgac
    51601 gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat
    51661 gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa cgattgctga
    51721 gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc
    51781 tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga aacgacccag
    51841 tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg ttcaggatta
    51901 ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga gatcccgttc
    51961 tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc tctggcaggc
    52021 aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc catgcaaaca
    52081 gtgacctatc cagtacgtgt gatggccggg ctaggagccc attcagtcat tgtgactaat
    52141 gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat taccgatcat
    52201 atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat gggccctaga
    52261 tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa agcagcagca
    52321 gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg accaacttat
    52381 gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt agggatgtcg
    52441 actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg aatctcttgt
    52501 attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca
    52561 actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac attattgtaa
    52621 taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa
    52681 gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt ttttggaaga
    52741 tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat ataaaggaga
    52801 acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct atgcccatga
    52861 attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg gtgcattgtc
    52921 aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca gttcttcgat
    52981 gatcgaaaaa aatttccaag cctttcattt tcctccaatt agtgatttca atctattact
    53041 gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa atgttctttc
    53101 ggaagattct ttctacaaag atgatttgac agagacattc caattagctg aacttggcgt
    53161 tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat accatgtgca
    53221 gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa caacagctgc
    53281 agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag cgatagcaga
    53341 ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca agcaaaaata
    53401 gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt ttgaacaccg
    53461 tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca atttcaaaaa
    53521 acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc aggagaacac
    53581 cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt tatagtgttt
    53641 tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa acaagtggtt
    53701 gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg atatgtttag
    53761 ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg tctagtaatg
    53821 ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact ttcaagtttt
    53881 ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt gctgcagaac
    53941 cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc cagatgtgta
    54001 cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt aatttaccat
    54061 agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga tctgaatatt
    54121 ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa gcaacatcaa
    54181 attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg attccttctg
    54241 gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat tcactaagac
    54301 cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata ggtttatatt
    54361 tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc catcgaaaaa
    54421 ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca gtgcccacgc
    54481 aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca ggtaaattga
    54541 tatatttata acatgttcca actgaaacac cgcattttga agaaataaat tggatcgttt
    54601 cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg atttttggtc
    54661 ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta cgttctttga
    54721 tcaatgcgca ctccatattt gctaggtttt ccattaattg gaaatagatt tttcccattt
    54781 cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct ttgaataatt
    54841 gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg caaatgacta
    54901 gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca gtagaatcat
    54961 tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca gataattgat
    55021 caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca ataattttca
    55081 tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa tgtagcataa
    55141 agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta ttggatgatc
    55201 aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct ttttctgagc
    55261 cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc ccaacaagtt
    55321 catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc agatcaatga
    55381 tgtaatgacc accatctgtg gttaggactt ctccgttttc gtctgtacgc aagacaggtt
    55441 ggaatccttt ttcttcaaaa atatgaagta gctgttggga gccataaggg atcacttcaa
    55501 ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg atccaaatcg
    55561 tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg ccgcctttga
    55621 tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg actacgggga
    55681 cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt gtcgcatttg
    55741 aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca atctcttcta
    55801 ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc tttacgaatt
    55861 tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc tttttttagt
    55921 ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga taccattctt
    55981 ttttattcca ataaagcttt ttcaaaaaaa attgcatccc ttgttgacat gttttttcat
    56041 tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac gtgctgactg
    56101 tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat cacaaaaaaa
    56161 gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac aaggaatgcc
    56221 tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc
    56281 tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa actcacctca
    56341 aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga actcagcttt
    56401 acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt atatcccagg
    56461 gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga
    56521 cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg gtgttaacga
    56581 ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat tttaattatt
    56641 aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat atttcggaag
    56701 gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt accagatcct
    56761 atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga cggaaaacgc
    56821 gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc tacaggtaac
    56881 gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct tgaagttaaa
    56941 gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc tgaacgccgc
    57001 gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga acacacaatg
    57061 gaagaacgct tagcaaaaga aattatggat gctgcaaaca acacaggtgc ttctgttaaa
    57121 aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca ctatcgctgg
    57181 taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt ccttattttt
    57241 agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt ttctttacaa
    57301 aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac tgctactgaa
    57361 cgtatcttgt actacactgg taaaatccat aaaattggtg aaactcatga aggagcttca
    57421 caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc tgctgctact
    57481 acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca cgtagacttc
    57541 acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt tcttgatgca
    57601 caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac ttatggtgta
    57661 cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt atattcagta
    57721 tcaactttac atgatcgttt gcaagcaaac gctcacccaa tccaattacc aattggtgcg
    57781 gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat gtatacaaac
    57841 gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga attagctgaa
    57901 gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact tactttgaaa
    57961 tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg ccgcgcaaca
    58021 gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa aggggttcaa
    58081 ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc agctatcaaa
    58141 gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag cgctcctttc
    58201 tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac tttcttccgt
    58261 gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt
    58321 gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat ctctgaagtt
    58381 tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg ggacacacta
    58441 tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc agttatccaa
    58501 gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc tttacaaaaa
    58561 ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc
    58621 atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg tcgtgaattc
    58681 aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc
    58741 actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata cggtcacgta
    58801 tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc
    58861 ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa agatgctatg
    58921 gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact ttacgatggt
    58981 tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc tatggcatta
    59041 cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc tgtagaagtt
    59101 gttatccctg aagattactt aggtgatgtt atgggtcacg taactgctcg tcgtggacgt
    59161 gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat ggttccactt
    59221 gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg tggtacattt
    59281 acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga aatcattaag
    59341 aaaaacggcg gaaaagctga ataatttctt aaaaccggat agtcctgcaa agagattatc
    59401 caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga tatgggcatg
    59461 tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat tgtattttta
    59521 ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt
    59581 ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat cacaactgta
    59641 ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg tgctccagaa
    59701 gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac agatacacgc
    59761 cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat gatcactggt
    59821 gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc aatgcctcaa
    59881 actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg
    59941 aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat ggaagttcgt
    60001 gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc tggatcagct
    60061 ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca
    60121 gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat gatgccagtt
    60181 gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt tgaacgtgga
    60241 caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca
    60301 acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc tggagacaac
    60361 attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca agttttagct
    60421 aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa
    60481 gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt ctacttccgt
    60541 acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc
    60601 gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa
    60661 ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga aatcaaagct
    60721 taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa
    60781 ggtctcagga tctttgttcc gagctttttt atttattgca ggatcaggaa aaaagacagg
    60841 agtattataa tagaaggaac gcgaaataaa agagtcagta ttttctaaat gaaactttat
    60901 gaaaacaaaa tgttttttac aagaaaaatc cttgctttgt gagtttatat atattataat
    60961 gacaaaggtg cgtttacttt agtgaattgc acagacgtaa tcagaatgat ttcgtcggct
    61021 aagaaacgag aggttgcgac acgcccggac gctttgccac gaacgagcgt gacggaaatt
    61081 ttcgtggagc tatgtctact tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa
    61141 aaaattcgta tccgtttaaa agcgtatgaa caccgtattt tagatcaatc agcggataaa
    61201 atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg gtccaattcc attaccaact
    61261 gaacgcagtc tttacacaat catccgtgcg actcataaat acaaagattc acgcgaacaa
    61321 ttcgaaatgc gtacacacaa acgtcttatc gacattgtga acccaacacc aaaaactgtt
    61381 gatgctttga tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa attataaaac
    61441 taaaacaaaa ataatggagg tgtactcatg accaaaggaa tcttagggaa aaaagtggga
    61501 atgacacaaa tcttcactga atctggtgaa ttaattccag taactgttgt agaagctact
    61561 ccaaacgtag tactacaagt gaaaacaatg gaaacggatg gctacgaagc tattcaagtt
    61621 ggttaccaag atatgcgtga agttttatca aacaaacctg cgaaaggtca tgttgcaaaa
    61681 gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa atgttgagct aggagaatat
    61741 gaagtaggaa aagaaattac agtagatgtt ttccaagcag gagacattat tgatgtaaca
    61801 ggtactacga aaggtaaagg attccaaggg gttatcaaac gccacggaca aagccgcgga
    61861 ccaatggctc acggttctcg ttaccatcgt cgtcctgggt caatgggtcc agttgcacct
    61921 aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc
    61981 caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat
    62041 gttcctggag ctaaaaaatc tttaatcact atcaaatcag ctgtgaaagc taaataattg
    62101 taggaagaaa ggaggaacta aggaatgccg aatgtagcat tattcaaaca agatggaagc
    62161 caaaacggtg aaatcacact taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt
    62221 gtttacgatg caatcgtaat gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa
    62281 aaccgtagcg ctgttcgtgg cggtggacgt aaaccatggc gtcaaaaagg aacaggtcgt
    62341 gcacgtcaag gatctatccg ctcaccacaa tggcgtggag gtggcgtagt ctttggacca
    62401 acaccacgtt cttacagcta caaacttcct aaaaaagttc gtcgtttagc aatgaaatct
    62461 gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac
    62521 gcaccaaaaa caaaagaatt caaacaagtt cttgctaact tatctatcga ctctaaagta
    62581 ttggttgttc tagaaaaagg taacgacttt gctgctttat cagcacgtaa cttaccaaac
    62641 gtttctgtag taacttctga taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg
    62701 ttagcaacac aaactgctct tactcaaatt gaggaggtgc ttgcataatg aacttactag
    62761 acgtaatcaa acgcccagtg atcactgaaa aatcaatgct tgccatggat gacaagaaat
    62821 acacttttga agtggacact cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg
    62881 cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta
    62941 tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag
    63001 attcaaaaga aatccaatta ttcgaagctg ctgaataagc actaatagat aactcattaa
    63061 attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa
    63121 catgacaggt tctgattttg ctgaaatcac aacatcaaca cctgaaaaat cattgttaca
    63181 accattgaaa aaccatgctg gacgtaacaa caacggccgc atcactgtac gtcatcaagg
    63241 tggcggacac aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt
    63301 tgcaactgtt caaacgatcg aatatgatcc aaaccgttca gctaacatcg cgttagtaca
    63361 ctatgaagat ggagtgaaag cttacatctt agcacctaaa ggactagaag tgggtatgaa
    63421 acttgtttca ggtccagaag cagatatcaa agtcggaaat gctcttccat tagaaaacat
    63481 tccagttggt actgttatcc acaacattga aatgaaacct ggtaaaggtg gacaattgat
    63541 ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg tattgatccg
    63601 cttgaactct ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa tcggttctgt
    63661 aggtaacgaa caacacgaat taatcaacat cggtaaagct ggacgctctc gttggatgcg
    63721 taaacgcccg actgtacgtg gtagcgtaat gaaccctaat gatcacccac acggtggtgg
    63781 tgaaggtaaa gctcctatcg gacgtaaagc accagtttct ccttggggtc aaccagctct
    63841 tggttacaaa actcgtaaca aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa
    63901 aaataaataa ttactttgac catgtgtcgc acattttgag aggaggttca ccatgggtcg
    63961 tagtttaaag aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc
    64021 aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag
    64081 ttttgttgga tataccatcg cagtttatga tggacgtaaa catgtaccag tttacatcca
    64141 agaagacatg gtaggacaca aattaggtga atttgcacct acaagaactt atcgtggaca
    64201 tgctgctgac gataagaaaa cgaaacgcta atttgagggg aggataaatc caaatggcag
    64261 aacaaatcac atcagctaaa gcaactgcaa aaacagttcg cacttcacct cgaaaagccc
    64321 gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat
    64381 tcacaccaaa caaatctgct ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg
    64441 cagaaaataa ctttgactta gatgttgaaa acttggtagt aagcgaagct tttgttaacg
    64501 aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca
    64561 aacgtacaag tcatatcacg gtagtcgtat cagaaaaata aggagggata atcagtgggt
    64621 caaaaagtac atccaattgg aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg
    64681 tatgctgaaa aagagtatgc tgagttctta cacgaagatt taagaatccg taaatttatt
    64741 tcaactaaac ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc
    64801 gtaaacattt caattcacac agctaaacca ggtatggtta ttggtaaagg cggttctgaa
    64861 gtcgaaagcc taagaaaaga attaaacaaa ttaactggta aaagaatcca tatcaatatc
    64921 gtggaaatca aaaaaccaga tctagatgca aaattagtag gtgaaggaat cgcacgtcaa
    64981 ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt
    65041 gctggcgctc aagggatcaa aactcaagta tctggtcgtc taaacggagc agatatcgct
    65101 cgttcagaag gatactcaga aggaactgtt cctcttcata ctttacgtgc ggacattgat
    65161 tacgcatggg aagaagcaga tacaacatac ggaaaactag gagttaaagt gtggatctat
    65221 cgtggagaaa ttcttccaac taaaaaaaac actgagaaag gagggaaata atcatgttag
    65281 tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag
    65341 gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc tgttgattca cattggatca
    65401 caaaccgcca aatcgaagct gctcgtatcg caatgactcg ttacatgaaa cgtggtggga
    65461 aagtatggat taaaattttc cctcacaaat cttatactgc caaagcaatt ggggtacgta
    65521 tgggttctgg taaaggggca cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca
    65581 tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt ctagcttctc
    65641 acaaattacc aatgaaaact aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg
    65701 aaggttaaag aaatcagaga attaaccact gccgaaatgc ttgatcaaga aaaacaatta
    65761 aaagaagaat tgtttaatct tagattccaa ttagcaacag gtcaattaga aaacactgca
    65821 cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct
    65881 aagtaattat ggaaggaggc cattaagcgt atgactgaag gaagaaatca acgcaaagtt
    65941 tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca
    66001 aagaaaaacc accctatcta tggcaaacgc atgaactatt ctaagaaata caaagtgcat
    66061 gatgaaaaca acacagcaaa agttggcgac atcgtgagaa ttatggaaac tcgtccatta
    66121 tcagctacaa aacgtttccg tttgttggaa gtagtcgaag aagcagttat tatctaataa
    66181 cacgagattt tcctatatag attgaatgca aaatctgaaa ggaggataca caacgtgatc
    66241 caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat tttgacgatc
    66301 aaagtccttg gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg
    66361 gttaaacaag caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc
    66421 gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat atattaaatt tgatgaaaat
    66481 gctgcagtga ttattcgtga tgataaaagc cctcgtggaa cacgtatctt tgggccagtt
    66541 gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt cactagcacc agaagtttta
    66601 taatcttgag acacttatca aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat
    66661 aaagttaaag ttatcactgg aaaagacaaa aacaaagaag gcgttgtact agcagcgttc
    66721 ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct
    66781 tctcaagctg ctcctcaagg tggaattgtt gagatggaag cgccaattca tgtttctaac
    66841 gtgatggtgg ttgactctac tggtgtagct ggccgagttg gctacaaaga agtagacggc
    66901 aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag ataaataaaa gtttaaggaa
    66961 ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca
    67021 ttgatggaaa aatttaacta tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc
    67081 atcaacatgg gtgttggtga tgcggtatca aacgctaaaa acttagataa agcagttgaa
    67141 gaattaactt tgatcactgg tcaaaaacca atgattacaa aagctaaaaa atcaatcgct
    67201 ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga
    67261 atgtacgaat tcttagacaa attggtttct gtttcactac cacgtgtacg tgacttccat
    67321 ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata ctttaggtat taaagaacaa
    67381 ttgatcttcc cagaagtaga ttatgattta gtagataaag tacgtggtat ggacatcgtt
    67441 attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca attaggcatg
    67501 ccattccaaa aataattaaa ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa
    67561 aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt
    67621 ccacattcag tttatcgtaa gttccatctt tgccgtattt gcttccgcga acttgcctat
    67681 aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa aagtaaattc gcataagaag
    67741 gaggtaaaag atcaatggtc atgacagatc caattgcaga ttttctaact cgtatccgta
    67801 acgcaaacat ggttaaacat gagtctttag aagtacctgc atcaaaaatt aaacgtgaca
    67861 tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca
    67921 aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa
    67981 acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa
    68041 aagtattgaa cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt gtcactgata
    68101 aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg taatcaagta
    68161 gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct
    68221 gaaggagtaa cagttactca agacggaaac aacgttacag ttaaaggacc aaaaggtgaa
    68281 ttgactcgta ctttttcagc agatatcaaa atgaatatcg aaggaaacga agtaactttc
    68341 actcgtccaa acgacagtaa agaaatgaaa acaattcatg gaacaactcg tgcgaacttc
    68401 aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact aatcggggtt
    68461 gggtaccgtg cacaattaca aggatcaaaa cttgtattga acgttggtta ctcacatcca
    68521 gtagaaatca caccaccagc tggagtaact gttgaagtac cagctaacac acaagtaatt
    68581 gttaaaggtg cgaacaaaga agaagttggc gaattagctg caaacattcg tggaactcgt
    68641 cctccagaac cttataaagg taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa
    68701 gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat agaagccagc agcaggaaaa
    68761 aggcatttgg ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta
    68821 tttttcgaaa cgtatttact gcttgaaata gtagtacaat aaaaataaag aggtgacaat
    68881 tgtgattaca aaaccagata agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa
    68941 caatatctct ggtactgctg agcgcccacg cttgaacgtt tttcgttcta acaaaaacat
    69001 ctacgctcaa attattgatg acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga
    69061 taaagaaatt tcaggtggaa caaaaacaga agcagcaaaa gctgtcggta aactagttgc
    69121 tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt gaccgtggtg gataccttta
    69181 ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa aatggactag aattttagaa
    69241 gaaggaggaa taccattcat ggtttatatc gatccaaaac atttggaatt agaagaccgc
    69301 gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct
    69361 gctttagttg ttgtcggtga caaaaacgga cacgtaggat ttggtacagg taaagctcaa
    69421 gaagttcctg aagcaatccg taaagcaatt gaagatgcga agaaaaactt agttgaagta
    69481 cctatggttg gttcaacaat cccacacgaa gtaatcggtg cttttagtgg tggtagaatc
    69541 ctaatgaaac ctgctgtaga aggttctggg gttgctgctg gtggaccagt tcgtgccgta
    69601 ttagaattag ctggggtagc agatatcact tctaaatctt taggctcaaa tacaccaatc
    69661 aacgttgttc gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga agaagttgca
    69721 gaacttcgcg gcaaatcagt tgaagaatta atcggataag gaggacaaca ataatggctg
    69781 aattaaaagt tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag
    69841 ttaaagcgtt aggtcttaca aagattaaca gttcagtggt taagccttct aatgaagcaa
    69901 ttaaaggcat gatcaatact gtttctcacc tagtggacgt tgaagaaatt taatttagaa
    69961 aaatcataac tagaacatta aggaggtgcc aaacctatga aacttcatga attaaaacct
    70021 gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga
    70081 aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta
    70141 ggatttgaag ggggacaaac accattgttc cgtcgtttac caaaacgagg attcacaaac
    70201 gtgaaccgta aagactatgc agtcattaat cttgatgtat tgaaccgctt tgaagacgga
    70261 gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga aaaagctgga
    70321 atcaaagtat tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc
    70381 tctaaagcag ctcaagaagc aattgaagcg gctggtggat caatcgaggt gatctaatgt
    70441 ttagactatt aaaagatgct tttaaagtaa aagacattag atcgaaaatc ctatttactg
    70501 tatttgtgtt gtttgtattt cgcctaggtg cacatattac tgtaccaggc gtaaatgctg
    70561 cttcattgca aaacctttct tctttgccat ttttcaacat gttgaacttg gtaagtggta
    70621 gtgcgatgca aaacttttcc attttctcaa tgggggtttc accatacatt actgcttcta
    70681 tcgtcattca gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag
    70741 gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta gtattggcat
    70801 ttatccaatc tgtcggtatc actgctggat tcaatcaata cgctcaatta ggtttcgtga
    70861 ataatcctaa cgtagcaact tacgtaacga tcggtttgat tctaacagcc ggtacgatgt
    70921 ttgttacttg gctaggagaa cagatcacag aaaaaggtat cggaaatggc gtatcaatga
    70981 tcatttttgc aggtatcatt tctagattgc ctgaaagtat cagagaaatt tatgaagatt
    71041 actttataaa tattgatcct tcagacatat ggaaatctgc tatttttgtg gccattttac
    71101 tagtagcggt cttgttgatc gtgacattcg taacattttt ccaacaagct gaacgcaaga
    71161 tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac aagcagctat ctgccgttga
    71221 aagtaaatgc agctggagtt attccagtta tctttgctag ttcgtttatt gctacgccaa
    71281 atgctgtttt acaagcattg agcggtagat tcggttctga aaactggtac aaagtgatga
    71341 tggagatctt taactataat acagcacctg gtgctacgat ttatacgatt ctgatcgttg
    71401 ccttcacttt cttctacgcc tttgttcaag taaaccctga gaaattagcg gaaaacttgc
    71461 aaaagcaagg aagttatata ccaagcgtac gccctggtaa ggggactgaa gagtatgtct
    71521 caagattact gatgcgttta agtacagttg gtgcagtctt ccttggttta gtggcattgc
    71581 ttccaatcat tgctcaaatg gtttgggatc ttccacaatc aatcggatta ggcggtacaa
    71641 gcttactgat cgttatcggt gtagctttag aaactgcgaa acagttagaa ggcttaatgc
    71701 tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt gttgaggttt cccctcaaca
    71761 cacgttctca cacatttatt aggagggaaa acaatgaacc tcattttaat ggggctgcca
    71821 ggcgcaggta aaggaacaca agctgaaaaa atcattgatg cttatggtat tccgcacatc
    71881 tcaacaggag atatgttccg cgcagcgatg caaaacgaaa cagctcttgg cctggaagca
    71941 aagtcttata tggataaagg tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa
    72001 gagcgactag ctgaaccaga tacagagaaa ggcttcttat tagatggctt tccacgtacg
    72061 ctagatcaag caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct
    72121 gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc
    72181 tgcagcaatt gtggagcaac ttaccataaa attttcaacc ctacaaaagt tgaagacaca
    72241 tgtgatcgtt gcggtgggca tgaattctat caaagagaag atgataaacc tgagacggtt
    72301 aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa tcttggctta ttataaagaa
    72361 caaggtttgc taaacactat tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta
    72421 caaaaaatca ttgaaaaata gcacgattgc ccgtagccga cttgcattat gcagttactc
    72481 ttgtcaagtt caataaatta tgatagaata tgtcagtccg acttctagag aatcctctag
    72541 atgatccatt ttgagaattt tgaggtggga actgcagttt ccgccatttt atcgtgtgaa
    72601 aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga
    72661 aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag aaaacggaca
    72721 ccaagttctt gctactgttt caggtaaaat tcgtatgcat tacatccgta tcttacctgg
    72781 tgacaaagta acggttgaat tgtctccata cgatcttaac cgtggccgta ttacttatcg
    72841 ctttaaataa ttgtactccg taaaactaca ggaggtatta tcatgaaagt aagaccatca
    72901 gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt
    72961 tgcccagcaa atccaaaaca taaacaacgt caaggataat tggaggtgct ataaatatgg
    73021 ctcgtattgc aggagtagat attcctcgtg ataaacgtgt agtagtttca cttacttata
    73081 tctatggtat tggtaacact actgctaaaa agatcttagc taatgctggt gtatctgaag
    73141 atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc tatccgtgca gaagtagata
    73201 aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg
    73261 aaatcggttc ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca
    73321 cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat
    73381 aattcttaag tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc
    73441 gtgtgaagaa gaatatagaa gcaggtattg cacatatcca ttctacattc aataacacta
    73501 tcatcatgat cactgatact cacggaaatg cattagcgtg gtcatcagct ggttcattag
    73561 gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca aatggcagca gaaactgcta
    73621 caaaagcagc aatggaacac ggattaaaga ctgttgaagt aacagttaaa ggacctggtt
    73681 ctggacgtga agcagcaatt cgttcattac aagctacagg tttagaagtg actgcaattc
    73741 gtgacgtgac tccagttcct cataatggtg cccgccctcc aaaacgccgt cgtgtttaat
    73801 gagtgccgct cattttgagt tttatcagta acgtcattga acaagacact tttcgttttg
    73861 aaaggggtaa agataagaat gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa
    73921 gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa gagggtatgg gactacttta
    73981 gggaattccc tacgtcgtat tttattatct tctttgccag gtgctgccat cactaatatt
    74041 caaatcgatg gtgtactgca tgaattctct accatcaaag gtgttagaga agacgtcact
    74101 caaatcatct tgaacatcaa aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc
    74161 cttgaaatcg atattaccgg acctgctaca gtaactgctg gcgatattat cgttgatagt
    74221 gatgtagaaa ttcttaacaa agatatgtac atctgtagtg tctctgaagg agctacgttc
    74281 catgctcgct taacagtgaa acctggacgt ggttatgttc aagcagatga aaataaaaaa
    74341 gaagatatgc caattggcgt tcttccagtc gattcaatct acacacctgt tcgtcgtgtg
    74401 aactatcaag ttgagaacac acgtgttgga caccgtgaag attttgataa attgacgatg
    74461 gagatttgga cagatggttc aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc
    74521 atgactgaac atttggatat ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc
    74581 atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta
    74641 gacttgtctg ttcgctctta caattgtttg aaacgtgcag gaatcaatac tgtacaagaa
    74701 cttacaaata aatctgagcc tgaaatgatc aaagtccgta acttaggacg taaatcatta
    74761 gaagaagtta aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga agattaaaac
    74821 cttttacgaa ggagggaaac caacgtgagt tatcgtaaac taggacgcac atctagccaa
    74881 cgtaaagcga tgttgcgtga tttaacaact gatttattaa tcaacgaacg tattgtgact
    74941 actgaagctc gtgcgaaaga agttcgctca actgcagaaa aaatgatcac tttaggtaaa
    75001 cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga agttgccagc
    75061 gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt tacaaaaact gttcaacgat
    75121 attgcacctc gttacgcaga acgtcaaggc ggatacacac gcattttgaa gacagaacca
    75181 agacgcggtg atggtgcacc aatggttgtc ttagaacttg tctaaaatca cactaataac
    75241 atcactttat agatgattct tttagagtgt tatgatgttg gaagatattt ttccgagtct
    75301 agctcagcgc tgctcctcaa gcgaatatcg cttggggagt aagcactcat cataaagtgt
    75361 ttttttatac cttcaattac ctgtcatgac agatatgttt gaaggcataa aaaaacattt
    75421 taagcgagaa ataattgaaa attttgtctt tttttgatag tgttagtagg aactttagga
    75481 tgaagaagga gtaatcatgg agccaattat tgaattagaa aagataaatt ataaatacca
    75541 gccagatgat cttcgccccg cattgaaaga tgtctccttt acaattgata aaggtgagtg
    75601 gatcgctatt attggacaca atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg
    75661 attgcttctg ccagaatcag ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa
    75721 tatttggacg atccgacaga tggttggaat ggtttttcaa aatccagaca atcagtttgt
    75781 tggttcgaca gtagaagatg acgttgcttt tggtcttgaa aatcaaggga ttcccagaga
    75841 agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt agaatggctg agtttgcttc
    75901 acgcgagcct gctcgtttat caggagggca aaaacaacgt gttgccattg ccggcgtggt
    75961 tgccttacga cctgatatca ttattttaga tgaagcaacc agtatgctag atccagaagg
    76021 tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat
    76081 atctatcact catgatattg atgaagcagc taatgccaat cggatactag tgatgagaca
    76141 aggcgaactt gtccgtgaag gtacaccaaa agaaattttt tctgctggtc ctgaactgat
    76201 cgacttaggt ctagatctac catttcctga aaaactgaag agcgcactga aagaacgtgg
    76261 ggttgatgtg cccagtgaat atatgacaga agaaaggatg gtggattggt tatggacatc
    76321 cgttttgaac aagtagactt cacttatcag ccgaatacac catttgaaca aagagcctta
    76381 tttgacatca atatgacgat caaagaaaac agttatactg cattagtagg gcataccgga
    76441 agcgggaaat caaccttact tcagcatttg aatgcgcttg ttaagccaac aagcggtaca
    76501 gtccatattg gagaacgaga tattcagccg gatacggata ataagaattt aaagcccatc
    76561 cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac agctattcga agaaacggta
    76621 gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga agcactagtc
    76681 ctagcaaaag aaacattaga acaagttggg ctggatgaaa gctatttgga acgttcgcca
    76741 tttgaacttt caggagggca aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg
    76801 ccggaagtac ttgtattgga tgaaccaacg gcaggacttg atccccaggg tagaaaagaa
    76861 atgatggaga tgttttggcg tcttcataag gaacagcaga tcactattgt attagttacg
    76921 catttaatgg atgatgtagc caattttgca gattatgtct atgtgttgga aaagggacga
    76981 atcgttaata gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa
    77041 caactaggcg tacctactgc caccgagttt gcagaagaat taatggctaa agggatgaac
    77101 tttgctacct tgcctttgac agctgaagaa ttagcagacg ctatcgtcca ttatgctgga
    77161 ggaaatgccc atgatgaata aattgatatt tggtcgttat atgcctggag attccttgat
    77221 ccatcgattg gatccccgag ctaaattatt agccagtttt tattttatcg gaatcatttt
    77281 tttagcaaat aattgggaaa cgtacctttt gttggcattt ttcacattac tttctgtctt
    77341 cttgtcaaaa gtagatttga aattttttat ccgaggcatt cgcccattga tctggctaat
    77401 cctcttcact gtagctttgc aggtcttgtt tacaagtggc ggagaaactt attggagttg
    77461 gggtgttttc catatcactg aatttggtgt gatgaacggc ctctttattt tctgtcgatt
    77521 cgtgttgatc atttttatgt cgactctact cacgcttact acaccgcctt tggaattatc
    77581 ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta cggttcccag tccacgaaat
    77641 ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa
    77701 gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa
    77761 aatgaaagca gtcattcctt tgctgattcc tttatttgtc agcagcttca accgtgcgga
    77821 agatcttgcg actgctatgg aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata
    77881 tcgtgtattg cattggcata atagagacac gatggttatc attgcttttg tattattaac
    77941 tgtgctactg atctttttaa gaggataaac gttcagtggg ccgctatcta ttcttttctt
    78001 gctgatctgt ttgcaagaaa ggatgcgagc agtgtatgac aagaaatatt agggtctgtg
    78061 gcagactaag gtcacaggcc tctttctttt atctgaaaag aaagaacgaa aaaattttat
    78121 aaaaaaatca ttattccaga taacctttta ggaacaaagg agagaaaaac atggtacgtt
    78181 acaaagcaat cattgcatac gacggaacaa attttaatgg attccaaaaa cagccaaatg
    78241 ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa
    78301 tcacggtctt tggttcggga aggacagatg ccggagtaca tgcgattggg caagtaatcc
    78361 attttgatta tccagaggag cgtccgttag aacgaatgcg tttcgcgcta gatacccagt
    78421 caccagagga tattgctgta agacaagtag aaattgtttc agaagacttc cacgcaagat
    78481 atttggtcaa agaaaagacg tatcaatttc gagtggatat agggaaacca cgcagtcctt
    78541 ttcgacgcca ttatgcaagt tattttcctt accctcttga tttggaaaaa attcagcggg
    78601 cgcttcctga tttattaggg actcacgatt tcacctcttt ttgtgcatca ggaagctcga
    78661 tagaggataa agtacgtacg atctacgagg ctaagatgga ggtaaatgaa acaaaagacg
    78721 aattgttgtt tacatttagg ggaaatggct tcttgtataa gatgatacgg atcttagtag
    78781 gaacgctttt gaaaattggc aatggccgat tgcctgaaga tagtatccca gatattattg
    78841 cgaaaaaaga tcgtaatgca gcaggaccta cagcacatcc tgaaggactt tatttgtatg
    78901 aagtgaaata tgaataagag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
    78961 tttacgaaaa ttacttctca aaattttgca aaatttcggc ttattttcga aggagctact
    79021 tctggaacac cgtgaatcag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    79081 accaaacaaa aatagctcgt catatttttc gtttggttgg gcttattacc gcagatgcaa
    79141 gcttttgaac accgtttaag gaagaggttg tgaaatcagc gttttgacct gagtattaga
    79201 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat accgaaggtc
    79261 agctgattga acaccgttta agaaagaggt cgtgagcctg tcatttaact ctgagtcaca
    79321 agtaacaatt tcaaaaaaca gcttctcatc tttgatgaat atcgtgactt gtggacgagg
    79381 cgttgataag tgaatcatct ttttgttacc tattttttct gtaaccaaaa aacgatggaa
    79441 cgttgttttg attgccctta atgaggacag tcggaacagg tttgtcattt ttatatttaa
    79501 atggcacaaa tttttctaat tatttgtcat ttagcgctga aaatttttgc tatttatcta
    79561 aaaaagtgat aactacttaa gatgaagcag aatatttacg ccatacggca tacaaccgcg
    79621 aaaaatataa aaaaaagaaa acgattgaac gcgtttttgc agtcgcaaag gaaaagcatg
    79681 gaatgcgcta catcaagtat cgagacttgg aaaacaatca ggtgtatacg ctgattgttt
    79741 ttgctgcaat gaatctgaaa aagttgtcca cttggcagtt ggaacatatg tcttcatatc
    79801 ttttattata caaaaatagc aaaagcacca gtctacactc ttagtaaaga ggaaactgat
    79861 ccttttgtct acattcttaa agtacttaaa ctaagtactt tattttactc tttaggattg
    79921 atacagtagc atctatccct ttattaataa tcttttattt ttttaaataa agatgttgtc
    79981 tccatcttca ggcactaaaa ctttttcgga aattcctttt tctttattga aagactttaa
    80041 ttcttctctt gacagtcccc aatggtttac agcttccata tgaacggcaa taattgtgga
    80101 atctggtatt gctttatgca attcatataa atcgtctttc cccataataa gagattctcc
    80161 aactaaaaaa tgattatctc cagcatttgc tactatgatt tctggattat actgatcaat
    80221 agtttgttta acagcttcat accaaactgt atctcctgta aagtatagtg tcttctcagt
    80281 cgaatgtttg aaaacaatac cgcaaacatt acctacataa ttcaagattt ctcctctgcc
    80341 atgtttgcct tttgttttaa ccaattgtat agattcaaaa actgtgtctt ctcgtagtac
    80401 ctccacattt ttaaaaccat atagtcgaat ttcattcgca tcattttcat cttgcacaaa
    80461 taattttatt tctttaggta ggacctcttt cgctatatcg tcccaatgat ctagatgtaa
    80521 atgtgttaca ataacagcat caataccgtt gataatatct tcgactggca ttggcaaatc
    80581 aactagagga ttcatttgat cttttcttgg tgatggaaaa ggacctttcg aaataaatgg
    80641 tggaagtgtt cctttttccg ctaacattgg atcaattaaa aattttttac ctccatagtc
    80701 taagatagct gttgcattac gaatgtgatg tatattcata aacgttgtct cctttttgtt
    80761 gatatgaaat atttttagta taattcaatt ccatacaata aataaaccga acaaaaaaag
    80821 tcttttacta tttttactta atttatgaaa ggttgtttac catgttagac caaacagata
    80881 taataa
//
LOCUS       DAJSVF010000003        68316 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSVF010000003 DAJSVF010000000
VERSION     DAJSVF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.003"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(424..537)
                     /locus_tag="ON550_000177"
                     /pseudo
     CDS             complement(424..537)
                     /locus_tag="ON550_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
     gene            complement(682..1626)
                     /locus_tag="ON550_000178"
     CDS             complement(682..1626)
                     /locus_tag="ON550_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953294.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1847..3061
                     /locus_tag="ON550_000179"
     CDS             1847..3061
                     /locus_tag="ON550_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3953295.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3083..5098
                     /locus_tag="ON550_000180"
     CDS             3083..5098
                     /locus_tag="ON550_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR3953296.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5181..6554)
                     /locus_tag="ON550_000181"
     CDS             complement(5181..6554)
                     /locus_tag="ON550_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3953297.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6580..6864)
                     /locus_tag="ON550_000182"
     CDS             complement(6580..6864)
                     /locus_tag="ON550_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3953298.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6866..8881)
                     /locus_tag="ON550_000183"
     CDS             complement(6866..8881)
                     /locus_tag="ON550_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3953299.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9060..9365)
                     /locus_tag="ON550_000184"
     CDS             complement(9060..9365)
                     /locus_tag="ON550_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR3953300.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(9454..10485)
                     /locus_tag="ON550_000185"
     CDS             complement(9454..10485)
                     /locus_tag="ON550_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3953301.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10692..11567
                     /locus_tag="ON550_000186"
     CDS             10692..11567
                     /locus_tag="ON550_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3953302.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11719..12561)
                     /locus_tag="ON550_000187"
     CDS             complement(11719..12561)
                     /locus_tag="ON550_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3953303.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12653..14530)
                     /locus_tag="ON550_000188"
     CDS             complement(12653..14530)
                     /locus_tag="ON550_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3953304.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(14527..15948)
                     /locus_tag="ON550_000189"
     CDS             complement(14527..15948)
                     /locus_tag="ON550_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3953305.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15951..19340)
                     /locus_tag="ON550_000190"
     CDS             complement(15951..19340)
                     /locus_tag="ON550_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3953306.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19564..19965
                     /locus_tag="ON550_000191"
                     /pseudo
     CDS             19564..19965
                     /locus_tag="ON550_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(20171..21490)
                     /locus_tag="ON550_000192"
     CDS             complement(20171..21490)
                     /locus_tag="ON550_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCR3953307.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            21747..22217
                     /locus_tag="ON550_000193"
     CDS             21747..22217
                     /locus_tag="ON550_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR3953308.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            22439..23542
                     /locus_tag="ON550_000194"
     CDS             22439..23542
                     /locus_tag="ON550_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3953309.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            23763..24722
                     /locus_tag="ON550_000195"
     CDS             23763..24722
                     /locus_tag="ON550_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3953310.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            24892..25572
                     /locus_tag="ON550_000196"
     CDS             24892..25572
                     /locus_tag="ON550_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3953311.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            25662..26696
                     /locus_tag="ON550_000197"
     CDS             25662..26696
                     /locus_tag="ON550_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3953312.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            26710..27045
                     /locus_tag="ON550_000198"
     CDS             26710..27045
                     /locus_tag="ON550_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953313.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            27029..29509
                     /locus_tag="ON550_000199"
     CDS             27029..29509
                     /locus_tag="ON550_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953314.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            29565..31124
                     /locus_tag="ON550_000200"
     CDS             29565..31124
                     /locus_tag="ON550_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953315.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            31121..32485
                     /locus_tag="ON550_000201"
     CDS             31121..32485
                     /locus_tag="ON550_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3953316.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            32656..33726
                     /locus_tag="ON550_000202"
     CDS             32656..33726
                     /locus_tag="ON550_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953317.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            33741..34409
                     /locus_tag="ON550_000203"
     CDS             33741..34409
                     /locus_tag="ON550_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953318.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            34429..35007
                     /locus_tag="ON550_000204"
     CDS             34429..35007
                     /locus_tag="ON550_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3953319.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            35408..36319
                     /locus_tag="ON550_000205"
     CDS             35408..36319
                     /locus_tag="ON550_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3953320.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(36697..37614)
                     /locus_tag="ON550_000206"
     CDS             complement(36697..37614)
                     /locus_tag="ON550_000206"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR3953321.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            37816..38253
                     /gene="zapA"
                     /locus_tag="ON550_000207"
     CDS             37816..38253
                     /gene="zapA"
                     /locus_tag="ON550_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR3953322.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            38311..38859
                     /locus_tag="ON550_000208"
     CDS             38311..38859
                     /locus_tag="ON550_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR3953323.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            38974..41334
                     /locus_tag="ON550_000209"
     CDS             38974..41334
                     /locus_tag="ON550_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3953324.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            41421..41735
                     /gene="trxA"
                     /locus_tag="ON550_000210"
     CDS             41421..41735
                     /gene="trxA"
                     /locus_tag="ON550_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR3953325.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            41917..43695
                     /gene="uvrC"
                     /locus_tag="ON550_000211"
     CDS             41917..43695
                     /gene="uvrC"
                     /locus_tag="ON550_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR3953326.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            43840..44739
                     /locus_tag="ON550_000212"
     CDS             43840..44739
                     /locus_tag="ON550_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953327.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            44732..45994
                     /locus_tag="ON550_000213"
     CDS             44732..45994
                     /locus_tag="ON550_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953328.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            46155..48053
                     /locus_tag="ON550_000214"
     CDS             46155..48053
                     /locus_tag="ON550_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR3953329.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(48383..49102)
                     /locus_tag="ON550_000215"
     CDS             complement(48383..49102)
                     /locus_tag="ON550_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR3953330.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            49439..49771
                     /locus_tag="ON550_000216"
     CDS             49439..49771
                     /locus_tag="ON550_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953331.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            49758..51761
                     /locus_tag="ON550_000217"
     CDS             49758..51761
                     /locus_tag="ON550_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR3953332.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            51780..52250
                     /locus_tag="ON550_000218"
     CDS             51780..52250
                     /locus_tag="ON550_000218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR3953333.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            52271..52855
                     /locus_tag="ON550_000219"
     CDS             52271..52855
                     /locus_tag="ON550_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR3953334.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            52870..53856
                     /locus_tag="ON550_000220"
     CDS             52870..53856
                     /locus_tag="ON550_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR3953335.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            53846..54157
                     /locus_tag="ON550_000221"
     CDS             53846..54157
                     /locus_tag="ON550_000221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR3953336.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            54179..55960
                     /locus_tag="ON550_000222"
     CDS             54179..55960
                     /locus_tag="ON550_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR3953337.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            55953..57329
                     /locus_tag="ON550_000223"
     CDS             55953..57329
                     /locus_tag="ON550_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR3953338.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            57336..57971
                     /locus_tag="ON550_000224"
     CDS             57336..57971
                     /locus_tag="ON550_000224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR3953339.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            58001..59356
                     /locus_tag="ON550_000225"
     CDS             58001..59356
                     /locus_tag="ON550_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR3953340.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(59548..60942)
                     /locus_tag="ON550_000226"
     CDS             complement(59548..60942)
                     /locus_tag="ON550_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3953341.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            61034..62005
                     /locus_tag="ON550_000227"
     CDS             61034..62005
                     /locus_tag="ON550_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR3953342.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            62002..62784
                     /locus_tag="ON550_000228"
     CDS             62002..62784
                     /locus_tag="ON550_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR3953343.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(62953..63324)
                     /locus_tag="ON550_000229"
     CDS             complement(62953..63324)
                     /locus_tag="ON550_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3953344.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            63458..63901
                     /locus_tag="ON550_000230"
     CDS             63458..63901
                     /locus_tag="ON550_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR3953345.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(63944..64753)
                     /gene="yidA"
                     /locus_tag="ON550_000231"
     CDS             complement(63944..64753)
                     /gene="yidA"
                     /locus_tag="ON550_000231"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR3953346.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(64772..66139)
                     /locus_tag="ON550_000232"
     CDS             complement(64772..66139)
                     /locus_tag="ON550_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3953347.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(66123..66953)
                     /locus_tag="ON550_000233"
     CDS             complement(66123..66953)
                     /locus_tag="ON550_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR3953348.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            67116..67553
                     /locus_tag="ON550_000234"
     CDS             67116..67553
                     /locus_tag="ON550_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR3953349.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            67629..68249
                     /locus_tag="ON550_000235"
     CDS             67629..68249
                     /locus_tag="ON550_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR3953350.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        21565 a        12067 c        13926 g        20758 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacggt
      541 ttacacctat atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa
      601 aattcgctgc aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg
      661 ctttagtgaa agattttctt cttaatgttt atttctccat tcattgggag tacatgttga
      721 atacttcttg aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat
      781 ttcttccaac gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt
      841 tagaaccagt tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac
      901 cggattgtag ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc
      961 gatataatct aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct
     1021 ttgttgttct aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga
     1081 acggatctta tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt
     1141 caaacgactt gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt
     1201 tgatgcattt atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat
     1261 ctctgtagat aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt
     1321 gtattcgatt ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc
     1381 tatcctttct ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac
     1441 aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt
     1501 tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa
     1561 gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt
     1621 acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt
     1681 ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca
     1741 taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc
     1801 acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa
     1861 aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt
     1921 cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga
     1981 attattaacg actgctccct cttttatggt cactattttt gttttgatta gtagttttat
     2041 tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg
     2101 tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact
     2161 aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca
     2221 cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc
     2281 tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt
     2341 ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac
     2401 aactgaactt caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa
     2461 aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat
     2521 taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat
     2581 cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac
     2641 aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt
     2701 gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc
     2761 tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc
     2821 ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc
     2881 atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt
     2941 caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac
     3001 gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata
     3061 aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta
     3121 tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg
     3181 ttttgactgg ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag
     3241 atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt
     3301 agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc
     3361 aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga
     3421 cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg
     3481 ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga
     3541 atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg
     3601 agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct
     3661 atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat
     3721 aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga
     3781 ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca
     3841 agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc
     3901 cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg
     3961 gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat
     4021 caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac
     4081 tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa
     4141 agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat
     4201 ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga tatttattga
     4261 ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc
     4321 ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa
     4381 ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta
     4441 cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt
     4501 gacgcgtcat gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca
     4561 atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt
     4621 caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac
     4681 gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata
     4741 cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag
     4801 cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt
     4861 gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga
     4921 aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga
     4981 taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta
     5041 tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa
     5101 caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca
     5161 atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg
     5221 gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag
     5281 gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta
     5341 tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc
     5401 agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca
     5461 gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga
     5521 atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag
     5581 ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct
     5641 aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt
     5701 tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca
     5761 gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat
     5821 ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat
     5881 acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg
     5941 tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta
     6001 tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca
     6061 ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt
     6121 gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct
     6181 tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc
     6241 atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact
     6301 actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca
     6361 aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca
     6421 aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca
     6481 agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc
     6541 aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt
     6601 cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc
     6661 atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat
     6721 atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc
     6781 aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact
     6841 ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt
     6901 cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg
     6961 ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca
     7021 aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag
     7081 acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc
     7141 caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt
     7201 cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt
     7261 tttttggttg atccgataat tggatcattt cttctgttaa taattcagat agcataggac
     7321 gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct
     7381 tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt
     7441 gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct
     7501 gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag
     7561 taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta
     7621 agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac
     7681 tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc
     7741 tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac
     7801 caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc
     7861 ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta
     7921 aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca
     7981 ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt
     8041 ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg
     8101 atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc
     8161 cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt
     8221 ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta
     8281 attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca
     8341 aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa
     8401 atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata
     8461 ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt
     8521 ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct
     8581 gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat
     8641 aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa
     8701 atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat
     8761 attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg
     8821 ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca
     8881 cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt
     8941 ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg
     9001 atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat
     9061 caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca
     9121 accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct
     9181 acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca
     9241 gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct
     9301 tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt
     9361 gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg
     9421 cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat
     9481 gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac
     9541 tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc
     9601 aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac
     9661 accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt
     9721 cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac
     9781 gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt
     9841 tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg
     9901 tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg
     9961 acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac
    10021 ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct
    10081 ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg
    10141 gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc
    10201 tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc
    10261 ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag
    10321 atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg
    10381 ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata
    10441 gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt
    10501 ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg
    10561 gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt
    10621 aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg
    10681 gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag
    10741 agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg
    10801 aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa
    10861 tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga
    10921 ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc
    10981 agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc
    11041 agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct
    11101 ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca
    11161 agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca
    11221 atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg
    11281 aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc
    11341 agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg
    11401 attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt
    11461 tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac
    11521 aagaattgat gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca
    11581 tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc
    11641 tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat
    11701 tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata
    11761 tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt
    11821 gttctcggat actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg
    11881 ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac
    11941 gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat
    12001 ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa
    12061 agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat
    12121 aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc
    12181 tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg
    12241 gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc
    12301 ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt
    12361 tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg
    12421 ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa
    12481 tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag
    12541 tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa
    12601 aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct
    12661 gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac
    12721 cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg
    12781 gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa
    12841 atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt
    12901 acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg
    12961 ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat
    13021 atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt
    13081 ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg
    13141 acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt
    13201 tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag
    13261 gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag
    13321 tgacagaata atttactggg tcgatttctt tatttccatc atataaggca taagcatacg
    13381 ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg
    13441 ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa
    13501 tctcatattg aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt
    13561 tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca
    13621 ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta
    13681 cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa
    13741 tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt
    13801 tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt
    13861 ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt
    13921 cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt
    13981 tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt
    14041 gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt
    14101 cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag
    14161 ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca
    14221 aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa
    14281 attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga
    14341 attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct
    14401 ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc
    14461 tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat
    14521 gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc
    14581 gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa
    14641 tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc
    14701 agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg
    14761 tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc
    14821 atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt
    14881 ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa
    14941 tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga
    15001 gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt
    15061 gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc
    15121 atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat
    15181 gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa
    15241 cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac
    15301 aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg
    15361 ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata
    15421 caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat
    15481 attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt
    15541 atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt
    15601 aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat
    15661 ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt
    15721 gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt
    15781 ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg
    15841 taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt
    15901 aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc
    15961 cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact
    16021 agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc
    16081 gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct
    16141 tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga
    16201 ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt
    16261 tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt
    16321 ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc
    16381 gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc
    16441 tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt
    16501 cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt
    16561 gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga
    16621 taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct
    16681 ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt
    16741 tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc
    16801 gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct
    16861 ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga
    16921 agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat
    16981 ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag
    17041 tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg
    17101 tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg
    17161 tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca
    17221 atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat
    17281 ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga
    17341 tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg
    17401 actccgggag ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt
    17461 ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt
    17521 tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc
    17581 tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt
    17641 gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga
    17701 tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat
    17761 tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat
    17821 gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg
    17881 ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct
    17941 tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt
    18001 gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac
    18061 tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat
    18121 gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc
    18181 atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt
    18241 gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta
    18301 ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata
    18361 ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga
    18421 ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata
    18481 tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt
    18541 tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata
    18601 tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt
    18661 gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg
    18721 gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat
    18781 ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg
    18841 ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct
    18901 ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt
    18961 gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg
    19021 tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct
    19081 tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt
    19141 tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc
    19201 gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt
    19261 gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt
    19321 ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat
    19381 agtgaaaatt attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt
    19441 attatacgaa gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat
    19501 gaagttgatt ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa
    19561 catatgatca ggttgttgga acgaaactat agaaagatgg aaaaaatcat tcgaattctg
    19621 tccatttctg acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa
    19681 tctacatttg taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt
    19741 caatcttcac aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat
    19801 gttctgatgg aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc
    19861 aatccgggta aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt
    19921 acacgtaagt tgaaacaatg caatcaaatt ttagaaagct atttattcct gaataattca
    19981 tagctttaat tctttggcac attttattga aatatgtatc actaatatcc ctatcaactg
    20041 attttgacta aaaagttact tcaaacattc actgttcaaa aatattgagg aataaatttt
    20101 gggtgtaagg cattttgagc atacttctcc ctggataact tcccagtaaa aaaatcgtta
    20161 gattgttgga ttagatccat tgactggctc gcctcaggcg agcaaagacc ctgtagaatt
    20221 cattctgata cacatgataa ctagataatt tgagatagac tcgtctaccc gtttggacta
    20281 atttcccagc aactttcaga aacttcaatc gaatagaatg aatggtcatt cccttttgga
    20341 cttcttcaaa gccaatcgtt cttaagaagt tgactaagtt gtaagctatc aagctgagca
    20401 tcattcggac atgattctcc aaaaagcgag gactgtctgt tttgtcaaag taaaagccag
    20461 ctttcgcttc tttaatgaag ttctccattg tgccacgttt ggcatagata gaaaagatgg
    20521 tatcaggaga aacattttct gatagattcg tcacgataaa ttcatgtcga aagagtagtt
    20581 cgcccgcttc acgtgttgag cgtatacaca cgcgacgact ttgtgaccag gattgtgctt
    20641 gataagtggt cgagaagtac tgaacttctc gttcttccca cttttgatta tcgccataaa
    20701 gtactgattt ctcagctatt tgacctagtc tacgattatt cttcagtcga ataacataat
    20761 gactcttttt tgattcacac gaatcataca caccaggtgt agcgaacccg ctgtctccac
    20821 gaaccaagat gtcagtgttt ggtagagagt gattatagtg ctctaataaa ggtgtgagaa
    20881 actcctttac gccttttgat gtatattgat ttcctgaacg tagttcagct tttaagaagt
    20941 caccagtcaa tccgtcaaaa gctaccaatg gatggtaacc ataggtttgg tagtgtgtat
    21001 tataattcgt ttgttcttgg tgaccaaatg tatctgaatg ggtcgaatct aaatcaatga
    21061 ttaattccgt atcattacgg atgaggcgtg ctttatcgat aagttcttgg ttcaaagctt
    21121 gaagttcatg gatattctcc tcagatagtc gatctaaaaa tcgggaaagt gaagattgag
    21181 aagcgagttc tttcctatct aaaacagctt taaacacagg atcttgtctc aggagattag
    21241 ctgcagaatc agctgagtaa ccagcaatta attgcataat gaactgctca agtatggata
    21301 agttgtcatg agtaaaatac gctcgttcgt cttcaatgtg aatgtgttgc ttagccagtt
    21361 cagaaaaacc aaagatgttc atcagctctt tcactaggac gagacccgaa tcactcgata
    21421 attgaccacc tgtatgagaa atagtgatat ttgaattgaa ttttacttgg tttttgtgta
    21481 agctagtcat gaaaagaact cctttctttt ggttggttta gtcactttaa ccatagcaga
    21541 aaggggttct tttttcatca cttaacgggt gaagatgaaa aagtaattgt aagctgccgt
    21601 gaggcagttt cacatggttt taattaaaag tatgaattat tcaggtagaa tctctgaaga
    21661 ataaatcacg tgatcgtgac gataaaatac acttacgtca ggtgataaaa aatacataag
    21721 tagaaaacta agaggaggaa cagaaaatga aagaagttca attgatcaga aaaagcgaac
    21781 tatcagaagg tggttgcaat gcatgcggcg tagtagaagc aacaagctat acattaaagc
    21841 ttggtgccaa caaagcgata atctctgagt tgacagtagg cggtcttgtg gacagtctgg
    21901 cgcttgctga aggttttatt ggagaagata tttatgagat gttttcagaa atccgtcagt
    21961 taaaaaaagg ggaaaactgc atagaggttc atcatgagag cccgaatgtg agattcaaac
    22021 gtggtgataa tgagatgatc ttcaataatc atgtgtccga tcacaccgag ctatatggaa
    22081 tcgtgaatca aatactaact gagctttttg gattgggacc ttatgcgttc aaggaagaaa
    22141 acggtaatcc aaaattaaac gaagaatggc aagaaacgat tgaaatacaa agaaacaacc
    22201 cgcacttatt tcaataggca tccacagtat ttcgtaaaag ggactttgac acgacttttg
    22261 tcaaaatccc atcgtttttt atctatttgt gttctaaaca tttctttcag agtagtaaca
    22321 actggaatga ctgaaatgta aacagattat ttgcctgtat caatacggaa gagtaatata
    22381 aagtgtgtaa acgctatcat attattcatt caatttttta tagagggaag gcattgaaat
    22441 ggaaaaaacg tatcaagcag gtacaaatga aggaatagtg gattttatca atatggaaga
    22501 tttagaaata gccgcttcac aagtgattcc agctggtggt tatggttaca tcagcagtgg
    22561 tgctggcgac ttatttactt atcaagaaaa tgaacgggca ttcaatcatc agctgattat
    22621 tcctcatgta ttaagagatg tagaattacc cgatacaact acacattttg acaaagaaac
    22681 gctgactgca ccaatcatca tggcaccagt tgctgctcat ggcttagctc atgtgaaggc
    22741 ggagaaagct tctgcaaaag gtgtagcaga ttttgggacg atctatacag caagttctta
    22801 cgcttcttgt acattagaag agataagaga agcaggcggg gagaaagcac cgcaatggtt
    22861 tcaattttat atgagcaaag atgatgggat caatttggat attcttgaag tggccaagcg
    22921 taatggagca aaagcaatcg tattgacagc ggatgcaaca gttggcggaa accgtgaaac
    22981 agaccgcaga aatgggttta ccttcccact gccaatgccg atcgttcagg cttatcaatc
    23041 aggagtaggc caaacgatgg atgctgtgta caaatcttcc aaacagaagc ttagtccaaa
    23101 agatgtagaa tttattgctg ctcattctga tttgccagtc tatgtgaaag gtgtccagtc
    23161 agaagaagat gtctatcgtt cattagaatc tggtgctggc ggaatctggg tatctaatca
    23221 tggtggccgt caactagatg gtggtccagc agcgtttgat tcgttgcaat atgtggcaga
    23281 agcagtagat aaacgtgtac caatcgtgtt tgacagcggt gtacgacgcg gacagcatgt
    23341 tttcaaagca atcgcatcag gtgcagatct tgttgctatt ggcagacccg ttatttatgg
    23401 attgtcttta ggaggaagta caggagtcca tcaagtgttt gatttcttta aaacagaatt
    23461 ggaaatggtc atgcaattag ctggaacaca aacagtagaa gatatcaaaa aaataaaatt
    23521 gcgtgagaat cgttttattt aagaaacaaa tgacgaaaaa aataggagtg cggcagatga
    23581 tgccgtactc ctatttttat agttcaagga tgtcactaat agaataattt gaaacgaaaa
    23641 aataaaattg aaatattttt tcatacttcg ccgattgtaa aattaagcta gacaactaaa
    23701 aaaagtcatt tgtgctacac tcaaaatagt tccgacgaaa gaattataag gagtgagtac
    23761 aaatgaccta taaccatctt acaccaactg aactcgtaat gatagaagct tatttcaatc
    23821 aatcacaacc tgtttcaaaa gtagccaatc tgttacaacg ttctcgacaa actatttata
    23881 aggtctatcg ttttcttaaa tctggtggaa gcgcaatgag ctactataaa caatataaga
    23941 aaaacaaacg taactgcgga agacatccga ttgttttacc tgatgagcaa caggaataca
    24001 ttcaaaaaaa ggttgttcaa gggtgggctc ctgatgtcct tattggtcga gcagagtttc
    24061 ctattgcttg ttcggtacgc acactgtatc gaatgtttaa agataaaacc tttgacactc
    24121 acgatttacc aatgaaggga aaaagaaaac caaacggtca taaagaaaaa agaggaaaac
    24181 aagggtttcg ccggtcaatc cgagacagaa agacagacta tacccagttt gatcatgaat
    24241 ttggtcacct ggaaggtgac actatcgtgg gtggaaaaca taaaagcgcg gtcatcacat
    24301 tagttgaacg gctatctaaa gtcatcatta ccttgaaacc cgaaggcaga caggctaaag
    24361 acattgaaaa taggctgaat caatggtttc gttgtgtccc aaaaaactta tttaaatcca
    24421 tcacatttga ttgtggaaaa gaattctcaa attggaaaaa gattagtaat accaacgata
    24481 tttcaattta ttttgcggat ccaggaacac cttctcaacg aggattgaat gaacattcaa
    24541 atggcttatt acgtaaagat ggtttgtata aagctatgga ttttaatggt gtatctgaaa
    24601 cctttatcca atcggtggca tcgaaacgaa atcatattcc tagaaaatca ttacactatc
    24661 ggacaccaat ggaagtattt ttgagttacg tcacaaaaga tgatttgtct aacttaattt
    24721 gacaaacgaa acttaataaa aaaggaagaa tattcatgta aacgatttta ttgttttttt
    24781 ctcttttata caaaaagggt cataatttca agtaagcgga tgaattcatg aggatttttt
    24841 gcatactgtc tctactatac ttaggtcact aagaaaaagg agtgatttat tatgaaaaag
    24901 aacatgattg ggacattatt actttcaaca cttcttctag gcggcttggc aacaccagct
    24961 tttgctgagg gtcaagcgac ttcaaaagga gatattacct ttactgaacc aacaaatacc
    25021 gtggagcctc ttaaccctac tgatccttca aaaccagtag agccagcaga tccggaaaat
    25081 ccagcaactg gccagacagg tcctttaaca ctggatgtag ttcccgaatt gccatttggt
    25141 acacacgaga ttgaaagcgg aacaaaaacg tatcaagtag acgcttcaaa aaacgataca
    25201 ccttatctcc aagtatcaga tcgtcgaggt gtaggtgcag atggccaagc acaagggtgg
    25261 aacgtgacag tttcggtttc agattttgta aacggttcac aagttttgca aggcgctgaa
    25321 ttagattttg gaacatccac tgttaaaagt acgtcagata acgaatccac tggaccaact
    25381 agtcaaactg taactgggtt gagtaaagca agtgcagcga caccaatatt tacagcagca
    25441 aaagatcaag gtctgggaac atggctttct gtttatgatc cagcaaatat cacactgaaa
    25501 gtgccgaaag cagcagctgg aacattcacg gctgatttga cttggaacct cgtggctggc
    25561 ccagtagcat agagtatgaa ataaattcaa caaactgcgg tgaaaatact ataggtgaag
    25621 gattttttac cgcagttgtt gttgaaagga tgtggtggac aatgaatcaa agtaaacgac
    25681 tgtttgtttc gtttttttct attttaagtc tatttttcat ttttccgtca atcagccaag
    25741 caaaggattc agcaggagac tttggaatca aaccggtatt tccagagaac caaatagata
    25801 aagcaatcgg ttattttgat ttattagtgg caccggaaca aaatcagaca ctagaagtaa
    25861 ttatcagcaa ttcttcagat gaagaacgaa catttgaggt ctctgtcaat ccggcggtaa
    25921 cgagtgatgg tggaacgatc gattatagtc aaaaaaaccc aacgttagat gagacattgc
    25981 cgtttgatgt ccgtgacgtt ttgctcattg cgaaaaagga aatcaatgta tcagcacatg
    26041 cagaaacaac tgtacctata gaagtgaaaa tcccagcaaa aagctttaag ggacgtgtat
    26101 tagcgggtat tcatgtttca cctaaagaag aagctgaaac agaaaatgct acagaaggag
    26161 cacaaatcaa aaatcgtatt gcttataatc tcgcagtcgt tttacaagaa agtcaagaga
    26221 cgatagaacc ggatctgaaa cttttatcag gagatttgga cgaggtcaac gcaaagccaa
    26281 ctgttcagct tcgtttccaa aatcctcagc ctaggattat cagcaatttg atatttacga
    26341 gcaagatctt ttatgaaaat cagctttata ttgaaaatac gtctaatgca tttttagttg
    26401 caccaaattc gaattttcac ttgaatcttg atttagctgg tgataaagca aaagccggcg
    26461 attaccgcgc agaaatcata gcaaaatcag gcgacagtaa tgaatggcga tttacacaaa
    26521 actttacgat caaaaaagaa aaagcacaaa aagtaaatga aaattccgtt tttgcagtac
    26581 aagaacaatc tttcccatgg ctttatgcag gattggttgc tgcagttcta gtgttatggt
    26641 tgattttgct gtttttgtgg gtccacaaga aaaagaaaaa aggagaaaca acatgatgag
    26701 gtgatttcgt tgaaaacatc gatcttattg ggagggatta ttgcaagttt gctgttcttc
    26761 agctggcaag agccagtcca agcagatacc aaaagttcag gtacgattac attttatgat
    26821 ctaagtccaa ttccgcatcc aagtggttca gtcaatggcg catcaaaaca gcagacacag
    26881 ctaccgattt ataaagaatc taatcaacat ggcgtattcc cggagacgaa tgaacaaaag
    26941 atccgttttc tttatttgat cattggtata gaattatggc ttcttgcgtg gttgttgtta
    27001 tggagaagaa aggaaaagga ggatgaacat gttgctacaa aataaatttt cattttttcg
    27061 ctccttcttt tttacttcgc tttttgtttt aagtatattt atcttttcac cttcggcaga
    27121 agctgttgga gcaacgcctc ctgccatttc ttatgatcca tacacgtatg aaaatatcgt
    27181 acctccaaat ggtggacgta gtgcagtcaa tggcgggaag cttgtccaac taactggggc
    27241 taaccaattg cctaaattga ctgctactta tttgaacgat ggttcaaatg atccttggaa
    27301 aggtggcacg gtccgttttc attgggaaag ccatgtgaaa ctaacaaatg tacttgattt
    27361 ggtgaatatc actgcacaag gatttgcatt tgtggcgatc atcaatttgc cggaacatgt
    27421 atcaagcagt gatgtattaa aagcaattga ctggaacaac gcctacttgt atattgccgg
    27481 tgcccccatc aaggttacta gcggaagttt attatcagca ggaagccata ccttgcgttt
    27541 tgggatgggg tcgtgggata caaatactct attgacagat attattgcta ttattacgcg
    27601 aggcgaattc aacgtggacg atatcccgct tcaatttgat gttacagcta atgtcgcgaa
    27661 gatgacagaa aacggagata aatatgatac gagtccaaac aaaattttga ccaagggtaa
    27721 attacaacct tctatgtcta aaaaggccgt tttttctgtt gatttttatg attcgaataa
    27781 tattatccaa gatcgtatgt tcaattcgat tttagctcca gctgcaggta gattttatcc
    27841 taaattaact ggcggaaact tgaatccagg agcatatgca ttttctgcaa gtgcagagat
    27901 aaatacgtgg aatagttaca tctcaccaag tgattcaaca ggaaaatata atacatttgc
    27961 tgataccaca gaatacgtgg atggaaataa ccagaacctt ccaggctcta ccaatagtcg
    28021 gacattgacg atggggctga ctgaaggacg ttttgatcag ttgccattta gtcgctttga
    28081 tcgcgtagtc aactatttta caggaaaaaa cgttacacaa ggagcaatac tgactcatac
    28141 tccagcaagc acaagtggat tgaatcagac agccaatatc gtgtacagtg ggaaagatgc
    28201 gcaaggcgga ttgctttctc ctgttgcttt aaaggtattg gagcagtaca agctagacgg
    28261 aacggtttta ccaacaaact taacagccga tagtcagtgg ggaaagctcc tttcttctgc
    28321 atcgtaccaa gtcacggcca attggtcagc aactgaacta aaaaagggtt cgatccatta
    28381 caggctttat tcgaaaggaa cccaaaaatt agcgacagga tggacagatc aacttttcca
    28441 aacgatccaa tcaaataacg ggggtgtaaa tacagctact tctacactac ctgcattggc
    28501 ttcagggcaa tattattttg attatcgctt ggtggacgat gtacttacta acatgtatcc
    28561 gtcttttgcg tacaaatggc agtctgaaca agctggaatc accactcttc ctcaaataac
    28621 agtggctaat tttccatctg tgagtgcaga tagtcagcta aaaaacctta gcagacaacc
    28681 tgagacagga gatcccctgt ctgctctatc aggagatacg attcaagaaa atgtgacatt
    28741 cacgttaaca aaatctgggg atgcgataac cgacaaaaaa atcacgatct cacttcctga
    28801 aaatactact tatatcaatg gttccttaaa attaaatggt acaactatcg cagataccgg
    28861 cattcaacaa ggagtttctg tcccagcaga tctattgagc aaaataggag ataccattca
    28921 tcttacttac aactatcaat taaatactgt agatacttct ttacaatccg tcagcatact
    28981 gacaaaggca gctgtacttt ccagcaatat tacattagca gatggtgcta agcttcctaa
    29041 cccagttgtc caaacatctg ctaaaacaat tcttgtcccc aaacaagaat taacattagt
    29101 taatgtacca gatgatttta catttggtaa cgatctcccc aaaccgttga agacaagtta
    29161 ttatgaatca aaaggcgatt tttcttttga tgtgcgggat actcggcttc caagcacgtc
    29221 tccttggcaa ctgacaggta cattgacttc tttatttaag aatgatcaag gtcaagaatt
    29281 aagcggaaca aaattatact tcaatcattc tggatcaaag caattgattc agcaaggtca
    29341 aaacacgttg atttatgaga gtgatggaac agctaaagga gaggttctcg ttgactttcc
    29401 cgataccgat ggtttactct tagaggtaaa tagcagtacg aatgctcaac ctggcgcaac
    29461 gtatcaaggc atggtcacat gggaactgac agctgggcca accagctaag tgattagaat
    29521 ttgtatggat ctgtgataac tgtcacagat cctctttttc aaggatgaga aggaaacttc
    29581 tgctgttaaa atcagaaatg cttgaaggag tggacttgga acttttcagt ttgcttgaaa
    29641 aaaatgaaca acggcaaatc aaacttttcc aacttttatt agaggaaaaa ggagaggttt
    29701 ccgttaataa cgtacttacc cgcttatcag ttgatcgggc taccttgaaa gaagatctgt
    29761 tttatttaca agctaacttg gaaaatatca aacaattgga aattgttatc caagaaggga
    29821 atattttatt aatcagacat ggaaatatag cagcagtcga tgtttattat cattgttact
    29881 tgcgctattc tacaaaatat cagatacttc tttatctgtt ggaaaatggc tcatttgatc
    29941 ggcagaaact tgtcatggaa cttaatatca gttcggctac tttgtctcgc agagtgaagg
    30001 aattaaatga ttctttaaat gagtttcatc tgaaaataaa aagcgggcgg ctgaccggtc
    30061 cagaaagtca aattcgttat ttttatttcc aattattttg gtttggacgt ccctatcttg
    30121 aaaacaaaaa agaatacagt gatttatcag gaaacacttt tttagatctg ctgcaaaaag
    30181 agtttagttt tgctattaca gaagaaggac gattgaaatt tgaaatatgg ctttccatta
    30241 tgaagaagcg aatgcgctgt acgaaaaaat ccaaaccaga ttatagcaat cttcctgttg
    30301 gattgtttca aacgacacaa tttttattgg acctccatac atttttgacc cgttacttct
    30361 ttcaatatgc ctttccttgg aatcaaaatg aaacgttggt tttttatctt ttcatgatca
    30421 gtaactttac attagataca cataactcgc atgtccagga gtttttgata agcgaaaaat
    30481 acaaagacga cgtagtagct caattgaatc gaacgtttaa acaatcgata gaagaatatt
    30541 tctcttttta tgtttttcca gaagagattt cgcaaaagat tgcgttgaca gtgatgcaaa
    30601 atcacgctca ttttgcctat tttagaggat ggatgaatat ttttggccaa gaaggattaa
    30661 aacagcggtt aactgaagta gaagataaac aattcctggt tttatgtgaa agattggtca
    30721 gacaaacatt tgctattgtt gattttactg aagtagacga ggaaaatgcc cgctttgaat
    30781 tatttgctcg ctatgcatct attttacgga cgctgatcaa gcagttagaa ttacgtctgc
    30841 aaattgctta tgatcttcct tatgaaaggc taatggcgga tatcgtgaaa gaaatcatcc
    30901 aggaacaatt gctgaatacg atcaaatatg atttagtcga atacgagaaa gaaaagcagt
    30961 atgatatcat tttgactagc caattgaaag agtacccgtc acagaagtcc gctaaagttt
    31021 ttgtattcac tagcaacgaa tataattatg atttccccta tttgaaccaa tttttaaaag
    31081 aatgttattt agaaaaattg aatttaagga tgaataaaac atgaaaatcg tgatcattgg
    31141 tgcttcccat gaaggcatca cagctgccct aaatctaaga aaacttcaac cgaaagcaga
    31201 agttttactg atcgatgaag atcataaaga tggactaggc tatgtttcaa atggaatcaa
    31261 cctttattta aaaggaaaga tacgtagttt agctgaagtt gcccataaca tgcggactct
    31321 tcaagaaagt ggggcaaaac tgatcaccga atggagagta actgaacttg atccagacaa
    31381 acatcagctc atcctcgcat ccaaagaagg aaaaaacgag accataacct atgataaatt
    31441 aatcgtagcg acaggttcct ctcctgtcac cttgtataaa caaattgaag ctgaaaatgt
    31501 ctacacctat aaaaatctag tgcaatctaa gcaagtactt gccgctttaa aagaagcaaa
    31561 agaagtagtg atcttcggtg ctggttacat tggattggaa ttagcagatg ctttgagaaa
    31621 taaaggatac attattcatc tagttgatta tatgccgaat gttttatcaa gatatttcga
    31681 taaggatatg atcaacagtt ttcaaaatca gctgcaaact aagcaaataa atttttatcc
    31741 aaatgaattc ttgatcgatt ggaaaaaatc agaagagaaa gtagtatccg tacaacttct
    31801 gtctcaaaca ataaaggcag atatggtcat tttttctgcc caaactcgtc ctaatacgac
    31861 attgttaaaa gaaaaagtgg ctttgtatga ggatgagact gtgatagtca atgaatatct
    31921 gcagacaagt gatccggaca tctatgcgat tggagatatc gtcccagtca gttttgacaa
    31981 aaataaacgg catctctttt tacctttagt tactcgagct gtacatatgg caagagcagt
    32041 agcgctaact ttgagtggtc agccaaccgc ttacgacctc agacaaaaga taacagcagc
    32101 agtcattata gactactttt taggaactgt agggctgaca gaagatgaag cgccattctt
    32161 ggaacaaaat accggttcgt gttcaggaga atttgatcta tttccacaat acgacgaaga
    32221 aaacaaaaca gtaaatgcaa agctgatcta ccatccggat acattagaaa taattggcgg
    32281 acagctgata agccaagaat tcttattatc agatttgaac ctgcttgcgg atatcgtgaa
    32341 acacaaaact acgattccac agctagcagt tgaggatttt ggatttctgt ctgaatacac
    32401 ctctcgtttt cactacctca atgagttagc ttttaaagta ctagctaaga gagcaaatag
    32461 caatagagta gataaaagac tgtgagaaat atcacggtct tttattgtgt ggggatataa
    32521 taaatattaa tcaatcgaga ataaaaaatt catataatga aatagcggaa gaattttttg
    32581 agcataatat tcgccaaaaa tttatcaagt gataaaattg ttgtataaaa tgattatttc
    32641 aggaggagga gttaaatgtt tttagcatta aatgaaatta tgcattcaaa gttacgttat
    32701 gcgttagttg caggggtgat gtttttgatc gcctacctgg tatttttttt gactgggtta
    32761 gcttatggct tagctcaaga taatcgaaca gcagtcgaca agtgggaggc agattcgatt
    32821 gtgctttcga aagatgcaaa ttcaaatcta gggatgtcca tgattactaa gaagatagca
    32881 gaagaagtcg aaggtgggaa agtggcgtat cttgcccaaa cacctggagt cgtgactagt
    32941 aaagacagta cagaagaagg aaaaatcaat gttagcttct tcggtatcga taaaaatcaa
    33001 ttcatcatgc ccaatcttgt tgaaggaaaa gcttttgata acgatgatga ggcagtaggc
    33061 gatattagtt tgaaagaaga atatggtcta gccataggag atacagttaa gttatctggt
    33121 agtgacaaga cgtttaaact aactggcttt acggatcatg ccaaattcaa tgtctcacca
    33181 gttctctata caaccatcaa tgcttaccaa gaaatccgct tcgaaaaaga agatacaagt
    33241 gaaaatgcac ggattaacgc aatagttgtc agaggtaaaa tcagtaattt acctgaagat
    33301 ttagaacaga tcaaaatttc taaattcatc aatgaattac ctggatataa tgcgcaagta
    33361 ttgacttttg gatttatgat cggatttttg atcgttattg cggctattgt gatcgggatt
    33421 ttcatttatg tactgacgat gcaaaagatt aatatctttg gtgtaatgaa agcacaaggg
    33481 atcacaggag gatttattgc gcgttctgta gtggcacaaa cattcatttt atcatttgtt
    33541 ggtattcttc ttggtctatt aggaacagtg ggaacatccc ttgttctacc tgatgcggtt
    33601 ccattccaaa gcaattggct gttttttggc gtcattagct tgttgatgtt agtagtagct
    33661 gtgttaggcg cactattttc tgttcggaca atcgtaaaaa tcgatccgtt gaaggcaatt
    33721 ggataagaag gaggaagaac atgaaagcaa tagaatttaa agaagtagaa aaaagtttcc
    33781 aagatggcga ccaagtagtt aaagcattga aagaaacaac catgagtatc gataaagggg
    33841 aatttgttgc aatcatcggt ccttctggtt caggtaaaag tacttttttg actttggcag
    33901 gcggattgca gacgcctagc agtggctctg tatttatcaa tgatcaagaa ttcagtaatc
    33961 aaaaagaaag cgaacgcgtt aaactacgtt ttaaagaaat tggctttatt ttgcaagctt
    34021 ccaatctggt tccttttctg acagtaaaaa agcagttatt attagtagat aagattaaac
    34081 atgaacatcg tcaaaaagaa gcaaacgaat tatttgagca gcttggcgtg gataagttga
    34141 tcaacaagta tccagaagaa ctatctggtg gagaacggca aagggtcgca atcgctcgtg
    34201 cattgtacaa tgatccgtcc attattctag cagatgaacc aactgctagt ttggattcag
    34261 aaaaggcacg agaagtagtc gatatcttgg caaaagaatc aaaagacaaa aataaagcaa
    34321 cgatcatggt tactcacgat acacggctga ttgacaaatg cgatagaata tttatgatgg
    34381 aagatggtgt attgaaagaa aaagaataac caaaaagcag gtggaaaaat ggtccatcga
    34441 tacaacaaag atacacagca aaaaattttg aaagaagcga ctgacttgtt tatgtccaag
    34501 ggatatctcg gcacttcaac aagagaaatc gcacaaaaag ttgggatcac ccagcccaat
    34561 ctctatcatt atttcggtga taaagaaaaa ctatatacgg cagttttaga aaaccactta
    34621 aaagatgttg gaaaagcttt gagagaaatc gttcaaacaa gcgaaacggg atttcaagta
    34681 accttgacaa aaatggcgca gtttttgatt gagacacatc tggtcgattt gtttctgatg
    34741 cttcacgatc ttgaaagcaa tctttcaaag gaaacaagag atcatctttt ctttctatgg
    34801 aagaaaaatt atcgtgaacc ttttgaagaa atcttttcag caaaccaatc tgtgctaaga
    34861 aatgggattt caaaagaaat cgcagcaagg cattttttct tagtgttagc accttacatc
    34921 actcggtcga aaagacctgt ggaagaaccg ctaaatgtgg cgcaattgat cgatttatat
    34981 ttgaatggtg tattaggaga agcgtaaagc tgttatatag ggagaaaata gttaggtgaa
    35041 gaagcaagat atgacgaaga tcgttatatc ttgcttcttt atataggtag tttttcataa
    35101 atacaaattt gttattctct catgattatc agggtatatt tttatttatt atgtcggtgt
    35161 tccaggaagt gttttaattt aataacgaag aaattaattg tttgtagaat gaaggaaact
    35221 atttattggg tagatgaatc accacgaata gtcaaaagga gatttcagga tatgccaaaa
    35281 atgaaaaaaa tcattttagc agtaatgtta ttttctacgg ttatatcttt tacagcccct
    35341 tttttgctta cttaagctga ttttaagttt tggcccttag tctggatcta cgaggtgaga
    35401 gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca atcattatca ttattatctg
    35461 ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga caaacggtca gcagtacgaa
    35521 cagtacggtt acaaagacaa aggaatcatt tgatgataca gaacaagaga cattaatcaa
    35581 aatcggaaaa aatgaccgta cactatatac agatgactcg ctaactaccc cacttgcaac
    35641 tataaacggc ggagagctgg cggagtttct ttctgaaaca aaaaatgctt atcagatcaa
    35701 gacaaatgat ggctatactg gatatttagc cctcgaagat ggaaccaaag tcacaaagaa
    35761 tatacaaaca aaaccggaag agctatctga tgcagtgatt gtattagatc ctgggcatgg
    35821 cggaaatgat actggggcat taagcaatga tgaacaaaca gaagaaaaag atataacatt
    35881 aagtacagcc ataaaagtaa aaaaggcgct agaggatgct ggagcaacgg tttatttgac
    35941 ccatactaca gatgaattag tacagcttgg agatatatgc gactacagtg aagaaaaaaa
    36001 ggcagatgtc tttatcagtt tacacgcaga ctctacggaa tatgcgaatg aagcaactgg
    36061 aatcacgaca tattattact atggacaaga ggaaacatta gcacagacaa ttgctgactc
    36121 cttcactgat ctgcctctgg attcaagagg aatctccact gggaactatc aagttcttcg
    36181 agaaaatctt caaccttcta ttttaatcga gatgggttat atgaataatg atagtgattt
    36241 aaaggaatta gtaacagaca gttatcagca gcagattgct gcaagtttga cacaagcatt
    36301 gaccacttat tttcagtaat aaataagccc agccaaacaa aaatagctcc tcatattttt
    36361 cgtttggccg ggcttattat cgcagatgca agcttttaaa caccgtttaa ggaagaggtc
    36421 gtgagcctgc cgttcaactc tgagtcacaa ggagcaattt caaaaaacag cttctcatgt
    36481 ttgatgacta ttgtgacttg tgaccgagga gttggcatga gaacaccgtt taagaaaaag
    36541 gttgtgaaat caacgttttg ttctgagtat tagagaaaca aacggaaaaa tagttcctca
    36601 tattttgccg tttgttgagc taataccgag gatcagatga ttgaacaccg tttattcgga
    36661 acaagggact gtgacatgac ttttgtcaca gttccattat ttttttagta gcttcaaagc
    36721 tttttctgta ttggcaaaat gaagtttggc atatttttca agcataggca tgccgccttt
    36781 ctccaatagt tttgccgcta cttggtcaga tttcgttcca gctcctgacg gaagggtgat
    36841 tccagcttca gctgatgctt tgtctaactc ttccaaaaag ctggcacgac tgatgataga
    36901 agcagcagca acagctaaat gatattgttc tcctttagta acgaagtaca gtttttcttg
    36961 taattgattt ttttctaaac gcgcatattt tttaaaattt gcttccggcg taaactgatc
    37021 gatcaaaatc gcttctggtt tttctggctc gatttttttt agcagcaaat ggatcgcttg
    37081 attgtgcaaa gcgactttca tccgaacggc attgtatttt ggttggatct cattgtattt
    37141 ttctggagat aagacaagca gtttataggg gatggttttt tttaagatgt cagcaagctg
    37201 gatgatttgg ctatctttta actcttttga atctctaaca ccaagtttac gtaattctgc
    37261 aagctgatca cgtttcacat aagcagcaca tactgttagc ggaccgaaat agctgccatt
    37321 tccgacctca tcactaccta aaacggataa ttgactgata ttttttggca aagaagaggc
    37381 tgtttttgct ggcttggctt gtttatcctg tgcctccccc catttatcag cttctattgc
    37441 tgcattcgta ccttgaaaca tcacttttcc ggatgtataa gcggtaatgg tcgtcccgtc
    37501 tttttttgca gcaaagctag tataaggaat ctgtttattt aacaaataag agcgatagta
    37561 tgctttcatt tgactaagtt ggtcgtttgt tgctttgata atgacgttgc tcataaaatt
    37621 ctcctcgttt ttatacataa aactataccg caactcacga cttttcgttt tctaagagat
    37681 tgcgagacat agcatttttt taaagtttat catataatag ttaagtatgt gaaaaatatt
    37741 tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa ataaaacttt catcaaatag
    37801 gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca aagcggtgat tgccaaccaa
    37861 acatatacta ttatcggacg ggaaaccaag catcatatgg atatcgtaac gaagctgatc
    37921 aatgaacagt tagcagaatt gaaacaatta tcaccacaaa tggataatga acaagcagca
    37981 atcttgatgg ctgtcaatgc gctttcggat caattgaaga aacaagaaag aatattggag
    38041 ctggaagaag aaacagcaga attaaagaaa aaaatgatca aatttacaga gcttgaaaat
    38101 cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg aagtattgaa agaaaatggt
    38161 caagcagatc ataccattaa aaatcatgtg gaagcacaac agattttaaa cgagaaacga
    38221 aaaggacaaa ttaaacaaaa atcttctaat taagatcctt ttatcaaaat aagaaaaatt
    38281 taaaaaagtt agaagagaaa ggaacgtagc atgctgagtt tgctgatttt atttttattg
    38341 ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt tgcaaatcat attcgcgatt
    38401 ggatatgtat tgtcatttat tgctgcccag catttttaca aaccattagc aagtcatttg
    38461 gaattataca ttccataccc agctgtcacc ccaacatcaa agttagcatt ttttgatcag
    38521 gtatttgcct ttcacctaga tgaagctttt tatgcaggaa ctgcttttct gattattttg
    38581 ctaattggct ggctgcttac ccgatttgtg ggggtttttg tccatggatt gacgtatgtg
    38641 ccgatcttga gacaggtaga ctggattgca ggaggcatcc tgtcactaat catggcttac
    38701 gttactatat ttttgatttt gcagttactt gcctttgttc cattagatgt ggtccaaaat
    38761 caattcggcg agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac
    38821 aagatgtatg agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata
    38881 tttcgttttt tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag
    38941 ctcttttttc atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg
    39001 gaatttgaaa aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa
    39061 gaactggcag aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa
    39121 actgaggatg gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaaattgaa
    39181 aatatccgtc cccatatgaa acggattgag atcggagcag acttgaatgg agtggagctt
    39241 gcacaagttt ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt
    39301 tctgacagtg aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc
    39361 cctgttttga gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac
    39421 gcttctcctg agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt
    39481 gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc
    39541 accatgcgaa atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc
    39601 ggtgtagtcc acgatcaaag tgcttcaggt cagactttat ttattgaacc aaaacaagtg
    39661 gtggatttga ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt
    39721 attttaagtg aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatact
    39781 tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa
    39841 gccatcgtgc cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca
    39901 ctgatcgatc aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc
    39961 atcgtcatta caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg
    40021 ttacaactga tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt
    40081 atttttgagg aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg
    40141 accttttctt ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt
    40201 ctggtattat ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca
    40261 atcgcaatct tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat
    40321 cctgaattga aagtttatgg atataatcgg gcaaatacga ttaatgccag catggagttt
    40381 gatgtggata cattaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat
    40441 gcgtttgaaa tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag
    40501 ttgatgaatg atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg
    40561 aaaatggcag agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt
    40621 cacgatgatt tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa
    40681 aaagcaaaga aaaaagcaaa tgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc
    40741 attgctgata ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag
    40801 ttgatcgatg caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat
    40861 aaagtattga agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt
    40921 acgacttacg gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa
    40981 atcggtatat taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa
    41041 gagccgacac aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat
    41101 caattggacc ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg
    41161 gattctgcca ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc
    41221 gcacttcgca agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag
    41281 tttgcaccgg caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta
    41341 ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat gctatactag tcatagattt
    41401 taaaaatgga ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa
    41461 cagataaagg acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc
    41521 aagcaccgat tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta
    41581 aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa
    41641 cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag
    41701 aacaactacg tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag
    41761 ggtgggactg aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa
    41821 ttaatatttt ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct
    41881 ataataactt taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa
    41941 ttagctttac ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc
    42001 atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggtagc
    42061 cataatacga aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca
    42121 gaatcgaata tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa
    42181 tacaacatta tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa
    42241 tatccccgtt tggtgattac tcgtaaggta ctaaaagaca aagcactgta ttttggacca
    42301 tatcccgatg tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg
    42361 agaaaatgca aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgc
    42421 ctgtgtcctt attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg
    42481 aaaaactttt taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa
    42541 aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc
    42601 attgagacaa tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta
    42661 tttggttatg cgattgataa aggatggatg tgtgtccaag tgtttttcgt aagacaagga
    42721 aagctaattg aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc
    42781 ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg
    42841 attcctgata cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa
    42901 ccaaaacgag gagaaaagaa aaaattagtt gaattggcga gtaagaatgc ctcagttgct
    42961 ttgaatgaac ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa
    43021 cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct
    43081 aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca
    43141 agaaaagaat atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca
    43201 atgagagaag tcatctatcg cagatattcg cgagtgattc gcgaagggtt gcctttacct
    43261 gatttgatct tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa
    43321 aatcaattag gaatcgatat tcctattgct ggcatggtaa aaaacgataa acataaaaca
    43381 aatgagttgc tattcggtac agatttggct gtagtaccgc ttgagcgaaa ttcaccagag
    43441 tttttcttac tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg
    43501 cagctacgca gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca
    43561 aaacgaaaaa aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg
    43621 acagaagaat tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa
    43681 ttacatcagg aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat
    43741 gtacaacaaa aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt
    43801 ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt
    43861 gttaaaactt ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga
    43921 aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc
    43981 ttggattttt tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag
    44041 aaagattatg atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg
    44101 attcaagacc aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa
    44161 ccaaaaatcg acttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta
    44221 aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa
    44281 cctaaattga tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg
    44341 ctaaaagatg gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac
    44401 aatatggata atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg
    44461 gttttgaatg gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg
    44521 gaatctggtt taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt
    44581 catgaagaca atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt
    44641 actcgtgcga ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa
    44701 gagattttca aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga
    44761 tgtctattta aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat
    44821 catgctgggt atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa
    44881 aatcggcgta ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga
    44941 tgattttgag ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga
    45001 aaaaatcgat gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta
    45061 tagcgaaaat tcattaggac aaagtacaca actactgata caacaacaac tgactggctt
    45121 gcaatcgatg cttcgagcga gtcaattagg catttcacca gaagaagtag ctagcttgag
    45181 ccaacaagca agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt
    45241 cggagaagat aactcgacag tccaatacgt cgtaagttat gtagcaacga ttgtcttgtt
    45301 cattattatt ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac
    45361 gcggatcatg gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaatt
    45421 aacaggtgtt ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat
    45481 aggatacagt caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt
    45541 ggataacttg attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt
    45601 gattttttct gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc
    45661 aaaagcaatt ttgccagtga cttatctatc tctaggcgga tacatgcttg gattgatact
    45721 aggagcttca gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc
    45781 ttcatatatt atgccgattc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat
    45841 ttccttgttg gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta
    45901 caaatcgaat gtactcgttt atagcgaagg cggattatgg agttcattga aacaatcgat
    45961 ttcaattatg aggaatgaac gaaaaaaagc ataaaacaat cctaaaatac agaaggaagc
    46021 tggacatcta ttgttcccag cttctttttg tatagaccta ttttatttat tttactatat
    46081 tggatatata ttcattgaaa atagtgtata acaactttat aatattgagt ataccaattt
    46141 ttggaggcgt tttaatggac cgttatttag atttactttc agaaaaattc ccctcaacgg
    46201 aggatgtcat cacggaaatc atcaatctcg aagcaatttt gcagcttcca aaaggaacag
    46261 agttattttt gagtgatata catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg
    46321 gttcaggaaa tcttaaagaa aagatcaaag accttttttc tgaacaaatg acggaaacgg
    46381 atatcagcca attcactatt tttacggctt atccagaata tgcattaacg actgattggt
    46441 acaaagaaca agataaatcg cagctgatcc atcatttgat cgatctttta cggtttacga
    46501 cagtcaaata tacccggtca aaagttcgaa aaggattgcc gaaagaatat agctatatca
    46561 ttgaggaact cttgtatatc gatgaccgta tcaatggaaa aaaagactat gtaaaaaaaa
    46621 tcattgaaca acttgttttg atgaaggaag aagaaaaatt tttaacgaaa cttgcacaga
    46681 cgatccaaaa atctgtcatc gatcatttgc atatcgttgg agatattttt gaccgaggaa
    46741 ctcaagcagc caaagtgatg gatcgaatca tggatttttc ttctgtagat atccaatggg
    46801 gaaatcatga tattttgtgg attggtgcgt attgcggctc tgaggcatgt ttactgacac
    46861 ttttacggat tgcagcacgc tataattatt tatttgaatt agagaaagaa tatgggttga
    46921 atttacgtcc attgttttct tttgctcatc aaacttatca gaagaatcca gtatttgcgc
    46981 ctaaaaacag taaaaatcta tccgaaagag agcaagaaga attagaaaaa atccatcagg
    47041 cactttctat ccttcaattc aaatcagaac atcagttatt ggatcgtcga cctgaattta
    47101 aaatggacga tcgtaaattg ctggaaaaaa tcgactatga agcatcaacg attgatttag
    47161 aaggtcagct ctatgagttg aaaggaactt gttttcaaac gatccagcca gatgatccaa
    47221 aaaaattctt ggaagaggaa caaaaagtga ttgatagttt gatgtattca ttccagcact
    47281 ctttgagaat gcaaaaacat atgacgtttt tgatcgaaaa agggggaatg tatctcacga
    47341 acaatcagca tgtgctgttt catggctgta tacctgtaga cgagaaggga cagctcttag
    47401 catttgaatt agaccaatct tatcgcggaa aagaactgct gggctttttt gaaaaacaga
    47461 taactgaaag cgcaaaagat ctgactgcaa aagaagattt agcaaccgat ttgatttggt
    47521 atgcgtggag tgggccattt tcgcctttat tcggcaagag taaaatggcg acttttgaac
    47581 gttattttct cacagagcct catacgcacg tcgagaaaag caatccttat tatcacttgc
    47641 gagatgaaga gtggttcagt gaaaaaattt tagcagaatt cgacacaaaa gaagaagggt
    47701 cagcaatcat taatgggcac acgccagtaa aagtaaaaaa aggggagtca ccgatcaaag
    47761 caaatggaaa agtatttgtc attgacggag gtttatccaa agcttatcaa aaaacgacag
    47821 gaattgccgg ttattcacta ttatgcaatt cttatggttt ccaaatcgtg acacattatc
    47881 cgtttttgcc tattgaacaa caatttgcaa aaaaatccga ccaaacaaat gtgaaaaaag
    47941 taatcgaaca gcaactacca cgcaaactca atcgtgatac aacaaaaggt atggaactgc
    48001 agcagcagat tacccagctc aaaaggctgt tggactatcg aaaggacgat tgagaagaat
    48061 tccagtgaac attgttccat agactggaga ctgagacaaa agtcgtgtcg cagtctcttt
    48121 ttccggataa acggtgttct tctgccaact cctcggtcac aagtcacact attcaacaaa
    48181 catgaaaagc tgttttttga aattgctcct tgtgactcag agttgaacgg caggctcacg
    48241 acctcttggt ataaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca
    48301 aaaatgtgaa aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt
    48361 cacaaccccc tcagaaagtc tattaaaatt cattcacaaa gaaatcgaat aagcgctgtt
    48421 cagatggatg tacacggtgg gtaagttccg gatgccattg aacaccgagg attttatgca
    48481 tatcatcttt gctttgaacc gcttcaacga gtccatcgtt agaaaaagcg atggctttta
    48541 ataaaggtga aagatctttg actgcctgat gatggtaaga attcacaaaa tatggatcgg
    48601 ggagaaggtt gcttaacaaa ctgtctgaaa caatcttgac ttcgtgagtc gtaaattgcg
    48661 gggcagtggg ctgttgttca tgttttactg accactttgg atataatgaa agatcttgat
    48721 aaagtgtacc ttccaaaacg acattcaata gttggagtcc ccggcagact gcaaaaattg
    48781 gttttctttg tttcaatgct tccaagatca gtgcctgttc aaattggtct cgttgaatat
    48841 ttgtttccgt tagctttgga tgaggatctt ccatgtaaag ttgtggactg acatcttgac
    48901 cacctgcaag aataagtttg tcgatttgtc caatcagttg aggagctgaa tctggagaca
    48961 tgactggcag aataagaggc aatccttgag catctaaaac tgcatccacg aatccttgcg
    49021 gtgtatagct cacttgattt ccttggaaag tatctgttgc tcgaatcagt tgatttccgg
    49081 caatgccgat gattggtttc atttgtattc cccctaaagt agacttattg taacaaggat
    49141 ttaaaaaaaa ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac
    49201 tatctgtaaa gagaaatttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt
    49261 tgttataata aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt
    49321 cttcaagggg tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag
    49381 aggtacatac tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt
    49441 gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgaaaagcg
    49501 tgctcaaaca ctgcggcagc agctgaagat ctatgcaaca gaaaaagagc aggaattgga
    49561 aacgatccaa acaaactatc gacaacaact aaaacagttg gtggaagaaa aagaacaatt
    49621 tgaaatacaa gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca
    49681 agcaactttg aaagatcaat atcaaaagca tcagagtcaa gcgatcggtg cgatcattga
    49741 aagggtgaaa gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata
    49801 aaagaaatca ggagaggatt ttacaagcag tacaaggaat ccaaaacgtt gaaatccggg
    49861 atctgtatca aaatagaaca aacaaccagt gggtggagaa gtattttcca gataccacga
    49921 ttattgataa agaagcaaaa ttaagcgctt taggtaatcg gttgaaagac atccaagaag
    49981 ctatccaatt catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc
    50041 aagaactttc cttacaagat ttggaagcca attattccga agaaaatttc ataaataaat
    50101 tgaaagaagt ccttgattta aaagaaaagt gggaacaact aactgaagaa cgtgatcgtt
    50161 tagaagaaga ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt
    50221 atgcaagtaa gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt
    50281 tcaaggaaga actgagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa
    50341 aacaagttta tctttcgtat gttgctttaa aaaaatcagt tgctgttata gaagaaattg
    50401 ctgggaaata cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaac
    50461 tgcaagagac caagcagcaa ttatccattg ttcacgagca gcaaaagaag ataacgacag
    50521 cgttaggtca gtgcagtggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg
    50581 ccgttgcaga acgagaaaaa ataaaagaac ggtttatcca tgcgacttat ctgatcgttt
    50641 tgcaaggttg ggtagatgca gaagaaaaac agaatttgct gcatgtgtta aacgaaaccg
    50701 tatcagaaga agatatatac gtcagtttcg aaacaccaaa ctcagaagag attcagcagg
    50761 aagtccctac taaattgaaa aaccatccgc ttgtcgaacc ttttgaattg ctgactgaaa
    50821 tgtacagtct gccaaaatat gaagaagtag atccaacacc ttggatgaca ccattttatc
    50881 ttgtcttctt cgggatgatg gtcgcagatg ttggttatgg tttgctgatg ctcattggga
    50941 caatactagc tcaaaaacta cttgttctac cacgcgggat gaaacgtttc gtaaaattct
    51001 ttgagattct ctcgatacca tcgatcatct ggggattgat ctacagttca tttttcggac
    51061 agtcattacc aaaagaactg tttggcatcc gcttgccttt cccaatccta tctacgacgg
    51121 atgacgtcaa tacgattttg attttatccg tgattttcgg tctgatacag attttggttg
    51181 gattatttgt ggcagcaaag gaaaatatca ggagaaaaga ttacttgagt gctgtcagtg
    51241 atggatttgc ctggcaaggg atcctaattg ggatcgttat tgcgttggtt ggtgccatgt
    51301 tgataaaaaa tacagcattt gtttaccttg gcgctgggct atcgatcatt gctgccttat
    51361 gtatcttgat cgttccgatt atccaaacgc cgtctaaagt caaaggtgct gccaaaggtg
    51421 cgtataatct ttacggactg accggctata tcggtgattt agtcagttat acacgtttga
    51481 tggcattggg tatttcaggc ggaagtattg gagcggcatt caatatgcta gttgctttca
    51541 tgccgccagc tgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga
    51601 atatgttttt gacactgcta agtgcctatg tccatggcgc acgattgcag tatgtcgaat
    51661 tttttgggaa gttttataca ggaggcggac gtgcctttga tccgctgaaa acggcggaaa
    51721 aatacgtcaa catcaatcac aagaaaaaaa acaataaata attggaggat ttttatataa
    51781 tgatggatta cttaattagt caaaatggtg gaatggtatt tgcggtatta gcgatggcaa
    51841 cagcgacgat tttttcaggg atcggttcag ctaaaggtgt tggaatgaca ggtgaggcag
    51901 cagcagcatt gacaacaagt caacccgaaa aattcgggca ggctctgatt ctgcaattgc
    51961 ttccaggcac gcaaggtttg tatggattcg ttattgcctt tttgattttt atcaacttag
    52021 gaaatgacat gtcagttgta caaggactga actttctagg tgcttctctg ccgattgctt
    52081 ttacaggctt gttttcaggt attgcccaag gaaaagtcgc agcggcaggt attcaaattt
    52141 tagcgaaaaa acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat
    52201 acgcgatttt aggtttcgtt atttccttcc tattagtgtt gaatgcctaa gcataaagga
    52261 ggaaaagcag atgaacgcca ttgaaaaaat catctctcag atgaacgaag cagcagagca
    52321 ggaaagagcg gcgttagaac aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa
    52381 acgtacgcaa gttgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattactcga
    52441 aaaaaaatac cgtcagctaa ggaatcgcca gcaagtagaa gtacgacaag aaaatttgaa
    52501 tgcgaagcag gaatttttaa gacgattgtt tgcagatgcg gtaactgaaa tggaaaattg
    52561 ggatgaatct gaacaaatcc agtttatcaa aaatgcattg tattcattac ctttgacagg
    52621 aaaagtagca ttcattgctg gagaaaaatc agcagcctat ctttctcaga cgttgttgga
    52681 tgaatggaac aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca
    52741 agcgggtttt ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt
    52801 acaagatatc caaggaacga tgagttttga gattgccaat caattattcg aataaggagg
    52861 gatcagtgta tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt
    52921 ttgtccaatg agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa
    52981 atattgaaat ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa
    53041 cagaatcttt taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag
    53101 cctgaaatcg tttggatcta tacgatgcgt tatactttcc ataatctgaa ggttttgaca
    53161 aaagctgaga tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca
    53221 ttagaaacct tgaaggatgc cattcatacg caagtttcaa atgaacttcc tgaaagcatt
    53281 atggacagta tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt
    53341 gacgtgattt atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga
    53401 tatcaagaat tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg
    53461 gctcgaggaa tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg
    53521 ggaagtatcg aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg
    53581 aattttctat tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat
    53641 attgatctcg taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa
    53701 gcacaaaccc aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta
    53761 gaaagcaaaa accttcgttt actggttatc ggcaaaagaa gccatttcga atcagaaaca
    53821 atcagaaagc gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga
    53881 ttctgttttg ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac
    53941 cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga
    54001 gcaatgtgca gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc
    54061 tatcgtattg attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa
    54121 aaacgtagaa aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt
    54181 gcaaattgga aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga
    54241 tgccagtatc caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga
    54301 gatgcgtggc gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg
    54361 cgaaccggtt cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc
    54421 acaaatgttt gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa
    54481 tttcttaggg cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga
    54541 accaacagta gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga
    54601 aacaaaagtt ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac
    54661 aatcgaaaaa ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg
    54721 tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga
    54781 aaaattaaac ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc
    54841 agtgacaaaa ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt
    54901 tcagcatcaa attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga
    54961 gcgtggaaat gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac
    55021 aggagaatcg ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc
    55081 agctcgagaa gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg
    55141 ttatgacgtt gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat
    55201 gagcggacgt ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg
    55261 gcttgcggaa tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga
    55321 aggaagtata accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt
    55381 cacacaaaat acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca
    55441 aaaaagacat tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt
    55501 agggaagtat atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat
    55561 gcgtattttg caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc
    55621 cctttcagat aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt
    55681 gcagcaaaat gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat
    55741 gttgaagatg atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta
    55801 tttaagtgag atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata
    55861 tatcccggaa acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat
    55921 gcaaacgatc gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg
    55981 aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa
    56041 tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag
    56101 ataaagcaat ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg
    56161 tacgattttt aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat
    56221 tcgatggttt gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg
    56281 atattaacgg agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa
    56341 caggtatctc tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat
    56401 tttctggctc tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg
    56461 tactcgattc atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag
    56521 aagctgaatt tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt
    56581 ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga
    56641 ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca
    56701 tgaccaacta tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa
    56761 gaagaggata tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa
    56821 gaatccgcgg actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg
    56881 ataaaactca tccaatccct gacttgactg gttatattac agaaggacag atcatcttga
    56941 ctagagagct ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc
    57001 ggttaaaaga taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga
    57061 atcagctttt tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag
    57121 gtgaatctgc tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa
    57181 aagaatatgt caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag
    57241 ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc
    57301 tggacaaata tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga
    57361 cccgcatgga attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat
    57421 tattaaaaga taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca
    57481 accagctgcg tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg
    57541 caaatgcttc gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg
    57601 ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg
    57661 aatatgatga acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg
    57721 aactggatcg gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag
    57781 aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca
    57841 atgcattaga atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga
    57901 agctggaaga aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta
    57961 ccactgacta agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca
    58021 aataaagaaa cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt
    58081 agttggcggc ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt
    58141 tatcgatgcg cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa
    58201 tacagcagag cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg
    58261 cggtcttggt tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag
    58321 tctcagcaca cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat
    58381 gaagctcatg ctttacatcc taaaattggc ctttgtgatc caattgattg gtacggttgc
    58441 tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt
    58501 ccatgcaatc tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa
    58561 tcaccagcaa aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt
    58621 aggcttcatc gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca
    58681 ttcaaaaatc gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt
    58741 tttcacagag cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa
    58801 cacctttttt atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca
    58861 tatgagccat gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg
    58921 gtcgacggcc ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc
    58981 gatgttgaaa ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt
    59041 gttgcgggca cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat
    59101 tttatcggtg actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt
    59161 cttctctgcg tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat
    59221 agggaaattg atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt
    59281 cttctctctg atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag
    59341 tgtcatgatt ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa
    59401 ataaaataat ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg
    59461 aggcactacc cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa
    59521 gatttgattc ctttatttat caataggtta taattcatat ttttttattt tttcttttac
    59581 gatacttagt ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa
    59641 tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc
    59701 aagaaagtca ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata
    59761 gtaagggaca cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc
    59821 tctttttgta agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat
    59881 ttgagtgatg acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg
    59941 ttctatcttg ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca
    60001 aataaatttt gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg
    60061 aggaatgatc gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac
    60121 ccatagacct tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg
    60181 aaaccctata ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct
    60241 acattcctga atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat
    60301 ctctaaatga tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc
    60361 tattctatca cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc
    60421 ggaaacaata gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc
    60481 tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag
    60541 atttgggtta ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa
    60601 agaactaacc agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat
    60661 ggactcaaag acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc
    60721 tttctcaatt ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc
    60781 agctagctct ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc
    60841 ttgttctaat tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg
    60901 tcgccaagtt aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa
    60961 ttgtatacca tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag
    61021 gagggaattg tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga
    61081 agaagcgatt caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca
    61141 aaatcctcga ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat
    61201 gcctgcttat ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga
    61261 taatcatcag tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa
    61321 cggactaccc aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt
    61381 atctgcgctg gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg
    61441 agctggtcaa caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac
    61501 agtgtttgtt tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa
    61561 acagtatcct aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc
    61621 ggaaatcatt tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag
    61681 ggaagacgca catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac
    61741 atcgttgatg gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag
    61801 cggggacctg cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac
    61861 attaggcgaa ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat
    61921 tttcgacgca gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa
    61981 attagagaaa ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat
    62041 gaggtgttca aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt
    62101 acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa
    62161 aaccaagaat ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac
    62221 gcgacattag caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt
    62281 aaattttcta ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg
    62341 gattttccta gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt
    62401 ggggctaaga ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa
    62461 aaaacggtgg ctaaattgca gccagatttt acagctatca aaaattttga attaggaata
    62521 ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca
    62581 aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat
    62641 tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg
    62701 gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt
    62761 tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga
    62821 gtggcaacct tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg
    62881 aaaagtttgc gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat
    62941 gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa
    63001 gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccagcagc
    63061 tttatgttgc tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc
    63121 gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc
    63181 cccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct
    63241 ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg
    63301 aacacatgtg tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa
    63361 caaaaagaag taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa
    63421 tggatagaaa ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga
    63481 aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga
    63541 tcagcagatt gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat
    63601 tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat
    63661 tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg
    63721 tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg
    63781 agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa
    63841 attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg
    63901 aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt acatattttt
    63961 tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt
    64021 tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca atcatggata
    64081 agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg
    64141 cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg
    64201 gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg
    64261 catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca
    64321 tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat
    64381 aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac
    64441 tgccgacacg gcgtgccatt acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac
    64501 gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt
    64561 aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac
    64621 ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta
    64681 cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta
    64741 gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac
    64801 ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg
    64861 acggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta
    64921 atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa
    64981 tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt
    65041 agctagaatt cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag
    65101 tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt
    65161 tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt
    65221 gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc
    65281 ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt
    65341 gatacaactc gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa
    65401 caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg
    65461 catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata
    65521 ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat
    65581 agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt
    65641 aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa
    65701 tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg
    65761 tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg
    65821 ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt
    65881 ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg
    65941 aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt
    66001 taatgcgtcg tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct
    66061 ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat
    66121 atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg
    66181 atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt
    66241 aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt
    66301 acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca
    66361 ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact
    66421 tgcccacgtt tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt
    66481 ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt
    66541 ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat
    66601 ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt
    66661 tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct
    66721 gaatttctga tcactggagc gtaacctgca tcttccagtg tttggatccc gtcacttaga
    66781 aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt
    66841 aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg
    66901 gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg
    66961 gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt
    67021 tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga
    67081 acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga
    67141 tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg
    67201 atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc
    67261 aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac
    67321 agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag
    67381 aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc
    67441 atgttagttt gaaagaccgt cccacagcag ggaaggttcg aatcgattat gatctgttca
    67501 tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa
    67561 agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg
    67621 tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg
    67681 aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta
    67741 accagatcca aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat
    67801 tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct
    67861 tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag
    67921 aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg
    67981 atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag
    68041 atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt
    68101 ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa
    68161 atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg
    68221 aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga
    68281 gacgatcagc ctatcagaat aaggaggtgt gacacg
//
LOCUS       DAJSVF010000004        64930 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSVF010000004 DAJSVF010000000
VERSION     DAJSVF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.004"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ON550_000236"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ON550_000236"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HCR3953351.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ON550_000237"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ON550_000237"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HCR3953352.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ON550_000238"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ON550_000238"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HCR3953353.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ON550_000239"
     CDS             complement(4028..5044)
                     /locus_tag="ON550_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953354.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="ON550_000240"
     CDS             complement(5146..5343)
                     /locus_tag="ON550_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953355.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="ON550_000241"
     CDS             complement(5315..6634)
                     /locus_tag="ON550_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR3953356.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="ON550_000242"
     CDS             complement(7375..8355)
                     /locus_tag="ON550_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR3953357.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="ON550_000243"
     CDS             complement(8368..9246)
                     /locus_tag="ON550_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3953358.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="ON550_000244"
     CDS             complement(9262..10062)
                     /locus_tag="ON550_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCR3953359.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(10337..10486)
                     /locus_tag="ON550_000245"
     CDS             complement(10337..10486)
                     /locus_tag="ON550_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953360.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            11454..11780
                     /locus_tag="ON550_000246"
     CDS             11454..11780
                     /locus_tag="ON550_000246"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3953361.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11761..12696
                     /locus_tag="ON550_000247"
     CDS             11761..12696
                     /locus_tag="ON550_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3953362.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13325..13576
                     /locus_tag="ON550_000248"
     CDS             13325..13576
                     /locus_tag="ON550_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HCR3953363.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="ON550_000249"
     CDS             14118..14276
                     /locus_tag="ON550_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953364.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="ON550_000250"
     CDS             14348..15574
                     /locus_tag="ON550_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3953365.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="ON550_000251"
     CDS             complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="ON550_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3953366.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="ON550_000252"
     CDS             complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="ON550_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR3953367.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(17034..17501)
                     /locus_tag="ON550_000253"
     CDS             complement(17034..17501)
                     /locus_tag="ON550_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR3953368.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(17621..19159)
                     /locus_tag="ON550_000254"
     CDS             complement(17621..19159)
                     /locus_tag="ON550_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR3953369.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            19239..19925
                     /locus_tag="ON550_000255"
     CDS             19239..19925
                     /locus_tag="ON550_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR3953370.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(20126..20653)
                     /gene="msrA"
                     /locus_tag="ON550_000256"
     CDS             complement(20126..20653)
                     /gene="msrA"
                     /locus_tag="ON550_000256"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3953371.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(20737..21414)
                     /gene="pgmB"
                     /locus_tag="ON550_000257"
     CDS             complement(20737..21414)
                     /gene="pgmB"
                     /locus_tag="ON550_000257"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3953372.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(21411..22541)
                     /locus_tag="ON550_000258"
     CDS             complement(21411..22541)
                     /locus_tag="ON550_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3953373.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(22541..24724)
                     /locus_tag="ON550_000259"
     CDS             complement(22541..24724)
                     /locus_tag="ON550_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3953374.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(24721..25746)
                     /locus_tag="ON550_000260"
     CDS             complement(24721..25746)
                     /locus_tag="ON550_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3953375.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(25791..26618)
                     /locus_tag="ON550_000261"
     CDS             complement(25791..26618)
                     /locus_tag="ON550_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3953376.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(26615..27682)
                     /locus_tag="ON550_000262"
     CDS             complement(26615..27682)
                     /locus_tag="ON550_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR3953377.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(27708..28544)
                     /locus_tag="ON550_000263"
     CDS             complement(27708..28544)
                     /locus_tag="ON550_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3953378.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(28541..29410)
                     /locus_tag="ON550_000264"
     CDS             complement(28541..29410)
                     /locus_tag="ON550_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3953379.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(29480..30763)
                     /locus_tag="ON550_000265"
     CDS             complement(29480..30763)
                     /locus_tag="ON550_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3953380.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(30777..32462)
                     /locus_tag="ON550_000266"
     CDS             complement(30777..32462)
                     /locus_tag="ON550_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR3953381.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            32737..33762
                     /locus_tag="ON550_000267"
     CDS             32737..33762
                     /locus_tag="ON550_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953382.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(34357..38031)
                     /gene="rpoC"
                     /locus_tag="ON550_000268"
     CDS             complement(34357..38031)
                     /gene="rpoC"
                     /locus_tag="ON550_000268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR3953383.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(38089..41706)
                     /gene="rpoB"
                     /locus_tag="ON550_000269"
     CDS             complement(38089..41706)
                     /gene="rpoB"
                     /locus_tag="ON550_000269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3953384.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            42434..43402
                     /locus_tag="ON550_000270"
     CDS             42434..43402
                     /locus_tag="ON550_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3953385.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            43420..44325
                     /locus_tag="ON550_000271"
     CDS             43420..44325
                     /locus_tag="ON550_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953386.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            44322..45128
                     /locus_tag="ON550_000272"
     CDS             44322..45128
                     /locus_tag="ON550_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3953387.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            45264..46253
                     /locus_tag="ON550_000273"
     CDS             45264..46253
                     /locus_tag="ON550_000273"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR3953388.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(46570..47094)
                     /locus_tag="ON550_000274"
     CDS             complement(46570..47094)
                     /locus_tag="ON550_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR3953389.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            47381..49192
                     /gene="scm"
                     /locus_tag="ON550_000275"
     CDS             47381..49192
                     /gene="scm"
                     /locus_tag="ON550_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3953390.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(49347..50333)
                     /locus_tag="ON550_000276"
     CDS             complement(49347..50333)
                     /locus_tag="ON550_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3953391.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(50356..50922)
                     /locus_tag="ON550_000277"
     CDS             complement(50356..50922)
                     /locus_tag="ON550_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR3953392.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(50936..51347)
                     /locus_tag="ON550_000278"
                     /pseudo
     CDS             complement(50936..51347)
                     /locus_tag="ON550_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
     gene            complement(51804..53738)
                     /locus_tag="ON550_000279"
     CDS             complement(51804..53738)
                     /locus_tag="ON550_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3953393.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            54019..54975
                     /locus_tag="ON550_000280"
     CDS             54019..54975
                     /locus_tag="ON550_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR3953394.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            55065..56168
                     /locus_tag="ON550_000281"
     CDS             55065..56168
                     /locus_tag="ON550_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3953395.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            56181..57134
                     /gene="menA"
                     /locus_tag="ON550_000282"
     CDS             56181..57134
                     /gene="menA"
                     /locus_tag="ON550_000282"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR3953396.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(57948..59294)
                     /gene="gor"
                     /locus_tag="ON550_000283"
     CDS             complement(57948..59294)
                     /gene="gor"
                     /locus_tag="ON550_000283"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR3953397.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(59428..60681)
                     /locus_tag="ON550_000284"
     CDS             complement(59428..60681)
                     /locus_tag="ON550_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3953398.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(60687..61610)
                     /locus_tag="ON550_000285"
     CDS             complement(60687..61610)
                     /locus_tag="ON550_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3953399.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(61831..64323)
                     /locus_tag="ON550_000286"
     CDS             complement(61831..64323)
                     /locus_tag="ON550_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3953400.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(64330..64797)
                     /locus_tag="ON550_000287"
     CDS             complement(64330..64797)
                     /locus_tag="ON550_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR3953401.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        18649 a        13882 c        11766 g        20633 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt
    15721 tcgaccatac gtgcgtcacg agtaagcaag ccagcacgtt taagcgctaa acggaagtca
    15781 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    15841 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    15901 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    15961 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    16021 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    16081 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    16141 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    16201 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    16261 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    16321 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    16381 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    16441 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    16501 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    16561 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    16621 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tctttttctt
    16681 tcttttatat aaaagggtcg tttcctgtta aaataaaaaa caaaaagagg ctgtgacaaa
    16741 agtcttgtca caacctctta ttttaaataa acggcggtct cctgccaact cctcggtcac
    16801 aagtcacaat atccataaaa cttaagaagc tgttttttga aattgtttct tgtgactcgg
    16861 agctgaacgg caggtttacg acctcttggt aaacggtgtt caatcagctg accctcggta
    16921 ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca
    16981 ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg
    17041 tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct
    17101 gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg
    17161 attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt
    17221 tctggaatag tacgatccca gcggccaatt tcatcaggga ttttcgtgtt gtctgcaaat
    17281 tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct
    17341 aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct
    17401 ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca
    17461 actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat
    17521 tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct
    17581 gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta
    17641 attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa
    17701 caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct
    17761 ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttcgcc
    17821 agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt
    17881 aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag
    17941 cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga
    18001 gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac
    18061 cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg
    18121 gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat
    18181 catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc
    18241 ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct
    18301 gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa
    18361 aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta
    18421 accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca
    18481 aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga
    18541 cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga
    18601 attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc
    18661 caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata
    18721 attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg
    18781 gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt
    18841 cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat
    18901 ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg
    18961 aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct
    19021 gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg
    19081 ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta
    19141 atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt
    19201 aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg
    19261 gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta
    19321 ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga
    19381 ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc
    19441 ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt
    19501 tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt
    19561 tgatggattt attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt
    19621 tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta
    19681 tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt
    19741 tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg
    19801 ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg
    19861 aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt
    19921 attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac
    19981 caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc
    20041 aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg
    20101 ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac
    20161 ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat
    20221 cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg
    20281 gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt
    20341 cttcttcatc attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc
    20401 ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa
    20461 taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta
    20521 cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga
    20581 tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg
    20641 ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg
    20701 ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga
    20761 taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc
    20821 agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc
    20881 ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg
    20941 ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc
    21001 aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt
    21061 gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat
    21121 tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt
    21181 tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa
    21241 tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt
    21301 ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg
    21361 agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta
    21421 cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga
    21481 ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa
    21541 acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata
    21601 gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt
    21661 agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt
    21721 ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca
    21781 aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact
    21841 tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt
    21901 tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga
    21961 ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt
    22021 ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct
    22081 tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta
    22141 cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt
    22201 tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc
    22261 aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt
    22321 tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg
    22381 tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca
    22441 gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc
    22501 agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca
    22561 agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt
    22621 tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt
    22681 tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa
    22741 gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc
    22801 ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca
    22861 ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta
    22921 tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca
    22981 aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa
    23041 gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa
    23101 tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat
    23161 acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta
    23221 aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg
    23281 ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt
    23341 catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca
    23401 gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat
    23461 cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt
    23521 ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt
    23581 tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt
    23641 gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat
    23701 tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc
    23761 agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg
    23821 gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat
    23881 cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag
    23941 acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt
    24001 tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa
    24061 aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt
    24121 ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg
    24181 tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact
    24241 gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt
    24301 cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag
    24361 caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc
    24421 gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct
    24481 caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca
    24541 ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc
    24601 ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa
    24661 tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt
    24721 tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg
    24781 gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt
    24841 cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga
    24901 aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg
    24961 atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt
    25021 cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt
    25081 gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg
    25141 gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc
    25201 aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa
    25261 cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc
    25321 cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa
    25381 tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc
    25441 agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa
    25501 tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc
    25561 attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc
    25621 ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt
    25681 ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt
    25741 cttcatttat ttctctctcc tttgcttatt tacttattgt tattctttct ctattctttc
    25801 tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc
    25861 tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca
    25921 tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt
    25981 tggtagcgat gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat
    26041 gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca
    26101 tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg
    26161 tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta
    26221 tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa
    26281 accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg
    26341 cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc
    26401 cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg
    26461 atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg
    26521 tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct
    26581 cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa
    26641 cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc
    26701 tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca
    26761 ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg
    26821 gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa
    26881 gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc
    26941 cgatccctat taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat
    27001 ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat
    27061 ctagcgtgta atcggcaccg tgtttttctg ctaattctct acgctgcgct atgggatcga
    27121 tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag
    27181 cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac
    27241 ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa
    27301 gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat
    27361 aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg
    27421 taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt
    27481 cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta
    27541 atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac
    27601 gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt
    27661 ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta tccttttacg
    27721 ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact
    27781 ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg
    27841 aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa
    27901 gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca
    27961 gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag accatcgatc
    28021 attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc
    28081 gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa
    28141 cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa
    28201 acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg
    28261 tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa
    28321 ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt
    28381 tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc
    28441 ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa
    28501 atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct
    28561 ttcgtgagag caagacgacc gctgcaataa tgatgaacaa tagaatcgat atagctgctg
    28621 cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga
    28681 ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa
    28741 caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc
    28801 aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa
    28861 tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca
    28921 taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc
    28981 tatctactaa cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa
    29041 aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc
    29101 taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg
    29161 ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac
    29221 ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg
    29281 gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta
    29341 ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg
    29401 gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca
    29461 ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga
    29521 catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga
    29581 tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa
    29641 aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt
    29701 aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga
    29761 cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt
    29821 tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt
    29881 gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag
    29941 aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt
    30001 tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg
    30061 acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat
    30121 ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag
    30181 taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta
    30241 aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat
    30301 accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg
    30361 aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt
    30421 tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac
    30481 tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga
    30541 acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat
    30601 cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt
    30661 gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa
    30721 atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat
    30781 tttattattt tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg
    30841 caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct
    30901 gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata
    30961 aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct
    31021 ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat
    31081 ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt
    31141 cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga
    31201 atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt
    31261 ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt
    31321 atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg ggcaccctct
    31381 ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga
    31441 ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg
    31501 aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga
    31561 agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca
    31621 aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga
    31681 gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt
    31741 atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa
    31801 gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt
    31861 ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg
    31921 tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta
    31981 tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat
    32041 tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt
    32101 gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga
    32161 taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca
    32221 actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt
    32281 tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa
    32341 tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata
    32401 gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac
    32461 atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac
    32521 gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa
    32581 agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa
    32641 gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta
    32701 ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt
    32761 gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat
    32821 atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg
    32881 agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat
    32941 cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca atccatttct
    33001 gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa
    33061 gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta
    33121 agctctacga tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg
    33181 gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc
    33241 caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt
    33301 ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc
    33361 ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta
    33421 gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt
    33481 ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag
    33541 cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt
    33601 tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa
    33661 cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc
    33721 gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa
    33781 cagaaacact gaggataagt cagttactct gttctttttt caatcactca tccctcgtta
    33841 ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga
    33901 taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc
    33961 acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc
    34021 atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt
    34081 tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct
    34141 atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga
    34201 cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga
    34261 tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg
    34321 tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc
    34381 gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac
    34441 accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat
    34501 cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc
    34561 agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt
    34621 ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc
    34681 tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg
    34741 taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat
    34801 tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc
    34861 acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc
    34921 gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat
    34981 acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc
    35041 tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat
    35101 cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg
    35161 agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc
    35221 aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga
    35281 gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt
    35341 gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc
    35401 ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc
    35461 tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc
    35521 gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac
    35581 atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc
    35641 tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca agacggatag
    35701 tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat
    35761 caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc
    35821 agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct
    35881 gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag
    35941 accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc
    36001 ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga
    36061 atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa
    36121 tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa
    36181 ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg
    36241 tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat
    36301 gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc
    36361 agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac
    36421 atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc
    36481 ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg
    36541 agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat
    36601 acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc
    36661 gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac
    36721 taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa
    36781 tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc
    36841 gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat
    36901 cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt
    36961 caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa
    37021 gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg
    37081 gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc
    37141 ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa
    37201 acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt
    37261 tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat
    37321 aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc
    37381 caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa
    37441 ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc
    37501 catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt
    37561 caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa
    37621 ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg
    37681 aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg
    37741 ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt
    37801 ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat
    37861 ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg
    37921 tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg
    37981 catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca
    38041 aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt
    38101 atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc
    38161 agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac
    38221 agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat
    38281 atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga
    38341 ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac
    38401 acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc
    38461 gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt
    38521 acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt
    38581 gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc
    38641 aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc
    38701 ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac
    38761 gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt
    38821 catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag
    38881 gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc
    38941 ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat
    39001 gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac
    39061 gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc
    39121 gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt
    39181 gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat
    39241 ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc
    39301 aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta
    39361 tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc
    39421 ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga
    39481 tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg
    39541 ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa
    39601 tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc
    39661 atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc
    39721 aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat
    39781 caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac
    39841 tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc
    39901 actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct
    39961 gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc
    40021 agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt
    40081 atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt
    40141 ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc
    40201 gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc
    40261 gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt
    40321 gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc
    40381 ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg
    40441 gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc
    40501 gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc
    40561 tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg
    40621 ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac
    40681 accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag
    40741 tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc
    40801 tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt
    40861 tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg
    40921 agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc
    40981 gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt
    41041 ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga
    41101 accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg
    41161 tacataagag atgtcttttg catcagtttc catttctagc caagctccac ggttagggat
    41221 aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc
    41281 tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc
    41341 ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt
    41401 ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc
    41461 acgagcttct gctactgtat actttggttc tttcaattca taatctacaa attccaaaga
    41521 tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc
    41581 atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac
    41641 ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc
    41701 agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca
    41761 ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa
    41821 aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt
    41881 ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt
    41941 tttgcaactt catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt
    42001 ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat
    42061 tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa
    42121 cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta
    42181 gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta
    42241 tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt
    42301 ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt
    42361 ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa
    42421 ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc
    42481 tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat
    42541 tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga
    42601 aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc
    42661 ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga
    42721 tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat
    42781 cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga
    42841 aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg
    42901 cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga
    42961 agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa
    43021 agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc
    43081 taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt
    43141 tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt
    43201 caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg
    43261 gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc
    43321 gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga
    43381 cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt
    43441 ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc
    43501 actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca
    43561 gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc
    43621 tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt
    43681 cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg
    43741 atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca
    43801 ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt
    43861 gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc
    43921 gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt
    43981 tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc
    44041 tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc
    44101 gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt
    44161 gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat
    44221 tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa
    44281 aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat
    44341 tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag
    44401 gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg
    44461 gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt
    44521 tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc
    44581 gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg
    44641 ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc
    44701 gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct
    44761 cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat
    44821 tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt
    44881 ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta
    44941 tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc
    45001 aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat
    45061 tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat
    45121 cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct
    45181 ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc
    45241 tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca
    45301 aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc
    45361 aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct
    45421 atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa
    45481 aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga
    45541 cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga
    45601 agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg
    45661 ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg
    45721 aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa
    45781 ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct
    45841 gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca
    45901 ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa
    45961 aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca
    46021 aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata
    46081 aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg
    46141 tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga
    46201 agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag
    46261 atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag
    46321 actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa
    46381 tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat
    46441 cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc
    46501 atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta
    46561 gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg
    46621 catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca
    46681 gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc
    46741 aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg
    46801 ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa
    46861 ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg
    46921 gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc
    46981 tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc
    47041 aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt
    47101 tcctcctttt tatttatcct acttactttt tcctaattct aggataccta cttatatgtt
    47161 actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga
    47221 aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt
    47281 aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga
    47341 gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt
    47401 actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga
    47461 aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt
    47521 gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca
    47581 tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct
    47641 tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa
    47701 ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt
    47761 ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac
    47821 tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt
    47881 tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac
    47941 tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac
    48001 cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat
    48061 cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa
    48121 cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga
    48181 tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt
    48241 tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac
    48301 gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat
    48361 atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag atacatctac
    48421 cacaagtgat agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac
    48481 aagtgatagc acggatacat cagcttcaag cgacagtaca gatactacct ctaccacaag
    48541 tgatagcacg gacacatcag cttcaagtga cagtacagat actacctcta ccacaagtga
    48601 cagcacggac acgtcagctt caagcgacag tacagatact acctctacca caagtgatag
    48661 cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagcac
    48721 ggacacgtca gcttcaagcg acagtacgga tacatctacc acaacaagtg atagcacgga
    48781 cacgtcagct tcaagtgaca gtacagatac tacctctacc acaagtgata gctcagattc
    48841 atctaccact acaagtgata gcacggacac gtcagcttca agtgacagta cagatacatc
    48901 ctctaccaca agtgatagct cagattcatc aacttcaagc gatagtacag acacgtcgtc
    48961 taccacaagc gatacacgtc atgcaagtgt ctccttctca aaaacaagtt cggataacag
    49021 gacgaacttt aacttaacta ataaaacaag caatgacaat ggaaacaaat caaaaagagc
    49081 acttcctaaa actggaagtc aatcgaataa ttggataaca ctcgcaggcg ttatattact
    49141 tgtaatcggt ttaagaatca cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat
    49201 tcaagataaa aaagagcaac gtcgctaacg gacgttgctc tttatttttg ctttttatca
    49261 taaatacaag aactacaatt caatttgtca aaaaacacgt agatttcctt ctgagaaatt
    49321 tatttacgta tcgaaccttt ccctattcag tctttgcgat ccaaaatcag ttgggtaagg
    49381 gaaaagagat tctcttttgt tcgaagagag gtggcaggta atttctttat ttccttcaaa
    49441 gccttttctg tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc tgtatgttca
    49501 acgattttgc gtacggcgtc gacatcttct tctgtcattg ccgctttctt ctctaaataa
    49561 ggtagtagct ttggtttgtt tttttgcaaa gagtagatca acggtaaaga atagacccct
    49621 tgacgcatgt cttctagtac aggtttgccg atttcctggc tctcttttgt ataatctaaa
    49681 atatcatcga taatctgaaa ggccatcccg atagaaagtc cgatatctcc tgcttttttt
    49741 gcgaagcgct cgcttgttcc actttcaaaa gcgccaacag aacaacttaa ttggaacagt
    49801 tcagctgtct tacctgaaat attttccaaa tattctttta cagaaagttc ataattatag
    49861 cgatcatcca tctgccctaa ttcgccattc aatacttttt ccatactgcg agaattcaat
    49921 tggagacttt tcaaggaagt ggagtaatca gacaatagtt taaaacagca gacaaataaa
    49981 tagtctcctg catagacaga aacgacatta ccaaactgac tacggatatt aggctgacca
    50041 cgtcgcagat ccgcttcatc aacgatatca tcatggatca gtgtggccgt atggagcatt
    50101 tcgatagaag ctgccaaagc gatggctttg tctcgatctt gctctggtcc aaattgtgag
    50161 aacaatagct gataggcagg acgcaacagc tttccgccgg catggatcgt ttctttgatg
    50221 gcttgttcga caggtttgtt tttcaggcgg acagtttctt ccatccgttt tagtgtttct
    50281 gtcaattctt tacttaaagc aggatagttt tcccacattg gatgtagctt cataaaaaaa
    50341 ctccttgtgt gacgattatt ttttggtaaa cattgggctc catttttgtt tggttccctg
    50401 ctcataatct ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat aattcgctgc
    50461 gatcccgatt gcaatccctg ataaaatccc taaaactgaa aggactggca agtacaacat
    50521 gactgtccat gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat tatgcatgaa
    50581 accgccggcc gcactaattc cgatgatact gacacgtttt ggtcctaatt gcttgaccaa
    50641 taacattcca aaataactaa gcaaagcacc actcatacta taaaggaatg tggaaatcgt
    50701 tccgcctagc aaagtagtca ggaatagtcg tagccaaagt agtaagaagc tgtcttgttt
    50761 tttcatggta aaaatggcaa cgatcgtgat tagattggct aagcctagtt ttgcaccagg
    50821 cgcaaaagaa aaaggataag ggatcatatt ttccaataaa ccaatgatca ctccttgtgc
    50881 aacaagtagc gaaatataaa tcattttttg taatttactc atggtgtgtg aaggtctaat
    50941 aaatcagact gtcttcgtca cctccatctg ttgaacggac ttcgatcact aatttatgtg
    51001 gcaaacagac gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg cagatctggt
    51061 catcgcaatc tgcttctttt atccgtactc gatcgccatc tatctcgatc aagttataat
    51121 ctccgtgttc atctttgtaa gtatatgtgt tgctctcttc tccagccatc aaagggaatg
    51181 ttttgatttc tgtgccatcc acacgcagta ccgcttcttt ttcttgcggt gttttgctga
    51241 agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat aataaaaaca
    51301 tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt tgttctcctt
    51361 tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca cctgtgtctt
    51421 gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta attctttggg
    51481 cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt gacagaagtg
    51541 ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt ttgtgaaatt
    51601 tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga ggttgtaaca
    51661 gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt attcctttaa
    51721 ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg aaatcttgca
    51781 ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg tctaccgtac
    51841 caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa cccaatcgca
    51901 cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt tacccagtaa
    51961 aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact gcatagccat
    52021 gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac catgaatttt
    52081 tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt tgtgaaccag
    52141 tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact caaaccaaaa
    52201 tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc agctgtggtt
    52261 gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc ccatgtccaa
    52321 gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact tggtttgaac
    52381 acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa accatagaaa
    52441 atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc tcgagtcacg
    52501 ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat ataataacca
    52561 gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat agctaaaaat
    52621 ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc aatagaaacc
    52681 ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat cgaagccacg
    52741 atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt
    52801 gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt tttatcttcg
    52861 tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc tgattctaat
    52921 ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag tgtgtgagtc
    52981 ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac gattggtgca
    53041 ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc agcatcattt
    53101 cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt gatttcgttt
    53161 ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc cccaatcatt
    53221 tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt acgcagttca
    53281 tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg aatccttaca
    53341 gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt accaaagtca
    53401 ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc taatttggtc
    53461 ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag ttgtacattc
    53521 ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc tacacgtccg
    53581 ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc gatcagagta
    53641 attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga aacaccggca
    53701 tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct ccttatagac
    53761 tatctatata atgaatatat gataattctt ttagtttgat acttattaca caatctccat
    53821 atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt caaattttaa
    53881 aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt acatgggtta
    53941 tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg aatcacaaag
    54001 aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta
    54061 actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg cgcagatgac
    54121 aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac tgcaaaaagc
    54181 acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg cacatataaa
    54241 ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac
    54301 ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa
    54361 gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga aaaatacatt
    54421 cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact
    54481 ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat ccaagctgct
    54541 caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa agacggcact
    54601 tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga aatgacagtt
    54661 gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg taaatcaaaa
    54721 caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact
    54781 tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct
    54841 gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat catgtacgca
    54901 caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt agataatatc
    54961 gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg acgatagcca
    55021 gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac
    55081 tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc agcaactcag
    55141 caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat ccatattctg
    55201 atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat
    55261 cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc acgatcaatc
    55321 aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt
    55381 ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat tctcaaggcg
    55441 gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt gatgacgcca
    55501 gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat cataagatcg
    55561 aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc atcgatttag
    55621 gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa aaacaaggag
    55681 tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac agcccaagag
    55741 gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc ggatctgttt
    55801 taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac gagcgttatt
    55861 tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt tatccgttcg
    55921 acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt gacgggttgt
    55981 ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga
    56041 ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg accgatccaa
    56101 tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac cgttctgaac
    56161 taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt gaaatacgaa
    56221 caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc gctttttttc
    56281 atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt tttgatttag
    56341 cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa gtatataagt
    56401 atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga aacatgattt
    56461 tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat acgggttggc
    56521 ttttactttt gatgggattt atctgttgtt ttattggagt attctataca ttcggaccga
    56581 ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa
    56641 tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat ctgacagtca
    56701 acttttcatc tggacaattt gatttggtag gaaatctttg ggctatagca gcaatcgtat
    56761 tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat aatttgcggg
    56821 atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc ggacgtcctg
    56881 cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt ttaatggggt
    56941 tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg ccaattgtga
    57001 agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc agctattcca
    57061 tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta acaatcatta
    57121 tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc aagtaataag
    57181 aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat taccgcagat
    57241 gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt
    57301 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgag
    57361 aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg tcctgagtat
    57421 tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    57481 ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca caattttttt
    57541 cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag aactttcttg
    57601 gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa tttatttata
    57661 caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt ttaaacatcg
    57721 ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc ttcgtcagtt
    57781 acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat tatcgaagca
    57841 atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga ttttctggtt
    57901 ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta tcgcatcgtt
    57961 acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc ttctttagtt
    58021 gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc aacaccaata
    58081 gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat ttcgcatttt
    58141 tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata aactttcaat
    58201 tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt tgcaattggt
    58261 ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc tttctttcca
    58321 ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt cagatctaat
    58381 ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg gtatttatct
    58441 actttgataa accctttttt gtccaattcc acatttgtat tttccagacc taatttttcg
    58501 gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt ttcaccattt
    58561 tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat atgatgtgcg
    58621 taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag cattttatca
    58681 aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc taaaccacga
    58741 aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc tctcttaggt
    58801 aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc aggaatggtc
    58861 attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata ggtttcaccg
    58921 tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc ctcgacatga
    58981 ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt ttctcgtctt
    59041 tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta acttgaggca
    59101 tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt aggtacacaa
    59161 ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca
    59221 tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat tacgatgtaa
    59281 tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct ttatttaacg
    59341 atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg cgaagatgct
    59401 taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat ctccaggttt
    59461 caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc ggtcaatgga
    59521 ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga atcctggacc
    59581 ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg tagcaatctg
    59641 tgttgcctca tcataagata atacttcacc gacaaattta tatactggga ttttacgacg
    59701 ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg tagctaattc
    59761 tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat agttttcagg
    59821 atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag atacataatg
    59881 gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg
    59941 aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt ccacttcgcc
    60001 tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc catacttcca
    60061 gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat ggttggacaa
    60121 tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt cgatttcggt
    60181 atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag ctaaaacgac
    60241 acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg ctgaagcttg
    60301 agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga tcaaagctgg
    60361 atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa attcccctgc
    60421 tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct tcgcatcatg
    60481 ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac ctgcacggct
    60541 tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat ggatcgcggt
    60601 ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca attttctttt
    60661 tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat ataaaagaat
    60721 cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt cattttttcc
    60781 gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc gaatagtcga
    60841 gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca tgtacagtaa
    60901 tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct ggcaaaaata
    60961 tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt aggttccgag
    61021 ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt atagtaattc
    61081 tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag ggtttctaca
    61141 ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc acgagcaagt
    61201 actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct cgttactaat
    61261 gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat cgtataaata
    61321 atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa agccaaatat
    61381 tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat attcgctttt
    61441 ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca
    61501 ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc
    61561 aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat aattttctcc
    61621 tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc ttctatttaa
    61681 agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata tattctgtcc
    61741 aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa gtcttactat
    61801 tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt cctttttagg
    61861 tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt ggtcacctaa
    61921 acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt tttgaagtgc
    61981 gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga tcacatcgat
    62041 tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga taatcgcttt
    62101 gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga agacgacagt
    62161 ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt ctaagatacg
    62221 tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga catttttttc
    62281 ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat ttctaaagtc
    62341 cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca gaagtaaatt
    62401 gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat atggtttttg
    62461 acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg gaggagaccc
    62521 gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac ggatcagggc
    62581 atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt ttccgacacc
    62641 agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca atccgcttcg
    62701 tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa cgacacgttc
    62761 atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca actgctgtag
    62821 agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta cacgatcaga
    62881 ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt tacgtagtgc
    62941 tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc
    63001 cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg ttaagtgatc
    63061 ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg ctttatcggg
    63121 tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta cagcttcatc
    63181 tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa gaatcacttc
    63241 tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct ctaatgcaga
    63301 atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg tttgtaattc
    63361 tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt ctgctccacc
    63421 tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat cattatagat
    63481 ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg taccagcaac
    63541 caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg gcacttcacc
    63601 taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac caggttcacc
    63661 caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc gtttcacttc
    63721 tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg tcaaatcacg
    63781 tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt gtacatttac
    63841 atttctacga cgattcattc cttttccagc agaatcacca gctagcccca ttttttgttt
    63901 cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc gagtagccat
    63961 gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt tttgagaacc
    64021 agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg
    64081 aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat gttcaatttc
    64141 ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag caattccatt
    64201 ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat gtttcaaccg
    64261 ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa aaagttcttc
    64321 catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc ggagaatccg
    64381 agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa ttgcactcaa
    64441 catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt gaataatagc
    64501 aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct caagtaattg
    64561 ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac cacgtttgct
    64621 ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt tgatttgtga
    64681 aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca tttcactatt
    64741 ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat gtgccatctt
    64801 attcattccc tttgactaat attgacctta attataacga atttgataat agaaagaaag
    64861 aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac ggaatatact
    64921 attccgtatc
//
LOCUS       DAJSVF010000005        63752 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSVF010000005 DAJSVF010000000
VERSION     DAJSVF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.005"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(160..900)
                     /locus_tag="ON550_000288"
     CDS             complement(160..900)
                     /locus_tag="ON550_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953402.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1030..1977)
                     /locus_tag="ON550_000289"
     CDS             complement(1030..1977)
                     /locus_tag="ON550_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953403.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2106..2870)
                     /locus_tag="ON550_000290"
     CDS             complement(2106..2870)
                     /locus_tag="ON550_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3953404.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2874..3263)
                     /locus_tag="ON550_000291"
     CDS             complement(2874..3263)
                     /locus_tag="ON550_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953405.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3398..3940
                     /locus_tag="ON550_000292"
     CDS             3398..3940
                     /locus_tag="ON550_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR3953406.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4088..4720
                     /locus_tag="ON550_000293"
     CDS             4088..4720
                     /locus_tag="ON550_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953407.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4854..4979)
                     /locus_tag="ON550_000294"
     CDS             complement(4854..4979)
                     /locus_tag="ON550_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953408.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5113..6006)
                     /locus_tag="ON550_000295"
     CDS             complement(5113..6006)
                     /locus_tag="ON550_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR3953409.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6188..7114)
                     /locus_tag="ON550_000296"
     CDS             complement(6188..7114)
                     /locus_tag="ON550_000296"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR3953410.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7199..7960)
                     /gene="pflA"
                     /locus_tag="ON550_000297"
     CDS             complement(7199..7960)
                     /gene="pflA"
                     /locus_tag="ON550_000297"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR3953411.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8204..10435)
                     /gene="pflB"
                     /locus_tag="ON550_000298"
     CDS             complement(8204..10435)
                     /gene="pflB"
                     /locus_tag="ON550_000298"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3953412.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10712..13162)
                     /gene="parC"
                     /locus_tag="ON550_000299"
     CDS             complement(10712..13162)
                     /gene="parC"
                     /locus_tag="ON550_000299"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR3953413.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13174..15219)
                     /gene="parE"
                     /locus_tag="ON550_000300"
     CDS             complement(13174..15219)
                     /gene="parE"
                     /locus_tag="ON550_000300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR3953414.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15385..15819)
                     /locus_tag="ON550_000301"
     CDS             complement(15385..15819)
                     /locus_tag="ON550_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR3953415.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15946..16584
                     /gene="plsY"
                     /locus_tag="ON550_000302"
     CDS             15946..16584
                     /gene="plsY"
                     /locus_tag="ON550_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR3953416.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16716..17591)
                     /locus_tag="ON550_000303"
     CDS             complement(16716..17591)
                     /locus_tag="ON550_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3953417.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17673..18464)
                     /gene="codY"
                     /locus_tag="ON550_000304"
     CDS             complement(17673..18464)
                     /gene="codY"
                     /locus_tag="ON550_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR3953418.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18482..19882)
                     /gene="hslU"
                     /locus_tag="ON550_000305"
     CDS             complement(18482..19882)
                     /gene="hslU"
                     /locus_tag="ON550_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR3953419.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19896..20444)
                     /gene="hslV"
                     /locus_tag="ON550_000306"
     CDS             complement(19896..20444)
                     /gene="hslV"
                     /locus_tag="ON550_000306"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR3953420.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20481..21386)
                     /gene="xerC"
                     /locus_tag="ON550_000307"
     CDS             complement(20481..21386)
                     /gene="xerC"
                     /locus_tag="ON550_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR3953421.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21584..22960)
                     /gene="trmFO"
                     /locus_tag="ON550_000308"
     CDS             complement(21584..22960)
                     /gene="trmFO"
                     /locus_tag="ON550_000308"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR3953422.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22929..25007)
                     /gene="topA"
                     /locus_tag="ON550_000309"
     CDS             complement(22929..25007)
                     /gene="topA"
                     /locus_tag="ON550_000309"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR3953423.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25129..25986)
                     /gene="dprA"
                     /locus_tag="ON550_000310"
     CDS             complement(25129..25986)
                     /gene="dprA"
                     /locus_tag="ON550_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR3953424.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26042..26809)
                     /locus_tag="ON550_000311"
     CDS             complement(26042..26809)
                     /locus_tag="ON550_000311"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR3953425.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26802..27662)
                     /gene="ylqF"
                     /locus_tag="ON550_000312"
     CDS             complement(26802..27662)
                     /gene="ylqF"
                     /locus_tag="ON550_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR3953426.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27922..28320
                     /locus_tag="ON550_000313"
     CDS             27922..28320
                     /locus_tag="ON550_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3953427.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28599..29099
                     /locus_tag="ON550_000314"
     CDS             28599..29099
                     /locus_tag="ON550_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3953428.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(29182..29733)
                     /gene="lepB"
                     /locus_tag="ON550_000315"
     CDS             complement(29182..29733)
                     /gene="lepB"
                     /locus_tag="ON550_000315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3953429.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29885..30112)
                     /locus_tag="ON550_000316"
     CDS             complement(29885..30112)
                     /locus_tag="ON550_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR3953430.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30109..30627)
                     /gene="msrA"
                     /locus_tag="ON550_000317"
     CDS             complement(30109..30627)
                     /gene="msrA"
                     /locus_tag="ON550_000317"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3953431.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30647..31360)
                     /locus_tag="ON550_000318"
     CDS             complement(30647..31360)
                     /locus_tag="ON550_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR3953432.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31363..32259)
                     /locus_tag="ON550_000319"
     CDS             complement(31363..32259)
                     /locus_tag="ON550_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3953433.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32417..33259)
                     /locus_tag="ON550_000320"
     CDS             complement(32417..33259)
                     /locus_tag="ON550_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3953434.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33391..34044
                     /locus_tag="ON550_000321"
     CDS             33391..34044
                     /locus_tag="ON550_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR3953435.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34107..34634)
                     /locus_tag="ON550_000322"
     CDS             complement(34107..34634)
                     /locus_tag="ON550_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3953436.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34654..35601)
                     /locus_tag="ON550_000323"
     CDS             complement(34654..35601)
                     /locus_tag="ON550_000323"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3953437.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35613..37499)
                     /locus_tag="ON550_000324"
     CDS             complement(35613..37499)
                     /locus_tag="ON550_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3953438.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(37659..39737)
                     /locus_tag="ON550_000325"
     CDS             complement(37659..39737)
                     /locus_tag="ON550_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR3953439.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39750..40025)
                     /locus_tag="ON550_000326"
     CDS             complement(39750..40025)
                     /locus_tag="ON550_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953440.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40387..40842)
                     /locus_tag="ON550_000327"
     CDS             complement(40387..40842)
                     /locus_tag="ON550_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953441.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(40996..42204)
                     /locus_tag="ON550_000328"
     CDS             complement(40996..42204)
                     /locus_tag="ON550_000328"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR3953442.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42316..42648)
                     /locus_tag="ON550_000329"
     CDS             complement(42316..42648)
                     /locus_tag="ON550_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3953443.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42796..43671
                     /locus_tag="ON550_000330"
     CDS             42796..43671
                     /locus_tag="ON550_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3953444.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43753..44334)
                     /locus_tag="ON550_000331"
     CDS             complement(43753..44334)
                     /locus_tag="ON550_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR3953445.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44340..45599)
                     /locus_tag="ON550_000332"
     CDS             complement(44340..45599)
                     /locus_tag="ON550_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3953446.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45601..46647)
                     /locus_tag="ON550_000333"
     CDS             complement(45601..46647)
                     /locus_tag="ON550_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953447.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46882..47157)
                     /locus_tag="ON550_000334"
     CDS             complement(46882..47157)
                     /locus_tag="ON550_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR3953448.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47400..48710)
                     /gene="der"
                     /locus_tag="ON550_000335"
     CDS             complement(47400..48710)
                     /gene="der"
                     /locus_tag="ON550_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR3953449.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48892..50121)
                     /gene="rpsA"
                     /locus_tag="ON550_000336"
     CDS             complement(48892..50121)
                     /gene="rpsA"
                     /locus_tag="ON550_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR3953450.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50256..50936)
                     /locus_tag="ON550_000337"
     CDS             complement(50256..50936)
                     /locus_tag="ON550_000337"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR3953451.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(50985..51620)
                     /locus_tag="ON550_000338"
     CDS             complement(50985..51620)
                     /locus_tag="ON550_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3953452.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51686..53089)
                     /locus_tag="ON550_000339"
     CDS             complement(51686..53089)
                     /locus_tag="ON550_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR3953453.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(53076..54107)
                     /locus_tag="ON550_000340"
     CDS             complement(53076..54107)
                     /locus_tag="ON550_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953454.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54138..54359
                     /locus_tag="ON550_000341"
     CDS             54138..54359
                     /locus_tag="ON550_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR3953455.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54524..55261)
                     /locus_tag="ON550_000342"
     CDS             complement(54524..55261)
                     /locus_tag="ON550_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR3953456.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55414..56754)
                     /locus_tag="ON550_000343"
     CDS             complement(55414..56754)
                     /locus_tag="ON550_000343"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR3953457.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(56991..58514)
                     /locus_tag="ON550_000344"
     CDS             complement(56991..58514)
                     /locus_tag="ON550_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR3953458.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58892..59503)
                     /locus_tag="ON550_000345"
     CDS             complement(58892..59503)
                     /locus_tag="ON550_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3953459.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59626..59751)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59833..60549)
                     /locus_tag="ON550_000346"
     CDS             complement(59833..60549)
                     /locus_tag="ON550_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3953460.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60555..61124)
                     /gene="scpB"
                     /locus_tag="ON550_000347"
     CDS             complement(60555..61124)
                     /gene="scpB"
                     /locus_tag="ON550_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR3953461.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61108..61905)
                     /locus_tag="ON550_000348"
     CDS             complement(61108..61905)
                     /locus_tag="ON550_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR3953462.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61875..62246)
                     /locus_tag="ON550_000349"
     CDS             complement(61875..62246)
                     /locus_tag="ON550_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3953463.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62265..63152)
                     /gene="xerD"
                     /locus_tag="ON550_000350"
     CDS             complement(62265..63152)
                     /gene="xerD"
                     /locus_tag="ON550_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR3953464.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63248..63721)
                     /locus_tag="ON550_000351"
     CDS             complement(63248..63721)
                     /locus_tag="ON550_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3953465.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT        17477 a        13459 c        10858 g        21958 t
ORIGIN      
        1 ttttattttg ccattatttt cttacttatt gggagaaaaa attcgcttaa actcaaaaaa
       61 atttgaggat acgatataaa ctttcggata gagtataaaa taaggaaacg ccgcagcgtt
      121 tcctttccaa ctgtagccga atataaattt gtatatcact tatttaaaat agctattttc
      181 tcccaaagag tttccttgat catcgatcaa tccttgtaat gagcgttcta agataccatg
      241 gctttttttc gatttttttt cttctgactg ctttgtttcc tctaacgttt ttgttcgatt
      301 gaatgttcgt acagacggct caccatttct tttttcttga tacggcgtcg gttctgtatc
      361 gaacaacaga taagagtcat ccgttttttt catcgattcg attaaatgat cctcagaaat
      421 tttgctttct ctagggtctg ggataatcga tgcgggtaca tatttaggaa caaaataaga
      481 acgctcccgc accttcgtgt caacagatgc gtttcgtgta gtatgcttgg actctgtcct
      541 tgtttcactt tgtctgacag aacgagagga atcaccaaaa agtttttttc gtcctgtcga
      601 tgtgatttct gattggtaat ggccggtata atccggtaat tttcctttat gcttttccaa
      661 attttcctta cgcggagctg acttcaatcc atttgttgca cgacgcttgc gacggatttt
      721 ctcggtgttc gtgttcgtga aatgaaattc tttcatctgt tcaaacgggg actgattctg
      781 ttcagtcaaa agatcttctt gtccgtcaaa taatactcgc ttcttgtcgt tggttaattt
      841 tacgcctaac tcatcatcgt acgcaggaaa acggaaatgt tttcgtctga tttctttcat
      901 tcaaaccatc cttctcaaaa atggaacttt tctcaataat agcacagatt cattgttttc
      961 agtagcaatg cgctgattat tttaagaaaa tagcgatgaa tttcataaat atatatatag
     1021 taataagact taattcgttg tctctctgat aactagttca ggaaaaaaac gacgataacc
     1081 ttgactcgcc cctttttctt ctattttttt taacaacatt tctccacata cttcacccat
     1141 acgtataata tcttgcttga tcgttgtcaa tttaggtgaa ctgatttggt ctaaaaatac
     1201 cccatcaaat ccaatcacac caaattcttc gggcaagtgt cctccagcat ttataatccc
     1261 tcttactgct cctagtgcta gccgatctga actacataca aaacaagttt tatctgtaaa
     1321 atctgcccag ttttctgtca tatatctttc tacttctgtc gaatgatttg gaaaccgcaa
     1381 gattttacta ggaagatcaa cttccttcat cacagataaa taaccagttt cccttgaaat
     1441 ttcaaaaggc tcgtccactt cgataccgat ataaataatc gtttgataat ctctttctaa
     1501 ggcatattgc gttgcttttg ctgtaccata ttcattatcg ctgtctacat aatcatatcc
     1561 ttcagaattt tccccaaaca agacaactgg tttttccaaa cggtcgatcc aatcgaagtc
     1621 tttttttctg acacctgtga tgatgtatcc atcacaagaa ccgacgtcga aggcatcatt
     1681 tgtaactaat tggagagaat aatgcgcttt accgatccgt ttagcgattc ccatcagcaa
     1741 attcatgtaa tagggctcag ttgtgtcgat ttcttccagt ataaataatt tgatgatctg
     1801 cgaccgattc gtgacaagtg cttttgcagc aatatttgga cggtaatcca gttcagccat
     1861 tgctgagaaa actaactctt tcaattcgtc agtcacctgt tccggatgat tgatgactcg
     1921 agagaccgtc atttttgaaa cattggcacg ttttgcaaca tctgctaaag tgggcatgct
     1981 ctcttccttt ctaacatgtt gtcattctta ctatcaatta taccaaattt tcctaacaga
     2041 gtttattata gcccaaaata aaaaacggaa aatcctctat cgtcatgagt gattttccgt
     2101 ttcaattatt tatacttttt tatcaaattt tttgctaaaa aagtggaagc ttcttccatt
     2161 agtggtattt gtaccatatg tcgctgtgtt tttgaggaac gaaaaacaat ttcttttccg
     2221 taagacttat gccgattttt ttctacaaat cgttccgttt gtatataagg gattttttcg
     2281 tcagcttgtc catgccaaaa gaacagagga cgtccagcta acttttccgg ctgcaatgac
     2341 aaatcatagc tcttaaccca tgaaatcaaa tcaaaatagt cttttggtac gacataatta
     2401 tattccgatg cgcgccttac gatttctttt ccatattcca aaggtgcagg ggaacccatc
     2461 aaacacacgc ccgctactat atgaggatgt ttagccaaca aggcgcatgt tgtcattccc
     2521 cccatcgaaa ccccgccaac tgcaagctga tcatgtagaa gacctctttt ttctaaatgc
     2581 ttgataagtg tgtcaaattc aaacagattt ccgtagatac tatcccaaaa tgtataggat
     2641 ggaatagaag aaaccggctg tttgcgctcc ccatgattca tcgcatctgg caggatcaca
     2701 cgaatgccct gctgcgcaat ttttcttcct tgcgtcaaaa ctaactcctt acttgtctgc
     2761 catccgtgat aatagatgac aacgggaata cgttcctctc ttaattcttt cagaacaact
     2821 tctaacactg gaatatgatg gacagaacgt ttcaatataa ttggattcaa ttgttactca
     2881 cctattttca ttaaatttcc tgatcgccat agcaaccatg ctccaacagt tgtctggaac
     2941 aggttcagtg taccaaataa tataaatgca ggactgaatg gctcgggaaa taagcacaga
     3001 gaacaaagtc ccaccactcc taatgctagc gaaaggagtg gataaataga aaaattcaaa
     3061 aaactggcaa cgctaccgaa aatattacca ataaacagta tcaaaaagaa aaaaacgctt
     3121 aaaattaaac catctgtcag cgacatctga aaaacaatgt tctctacctc aattggctgt
     3181 tgaatcgaat agacacctaa gtatatactg agaaggagac ttagcacgtg gctggcacaa
     3241 aacacacgat tgaccgtttt cattttaatc acctaaccta tctttataat tctattataa
     3301 tcattttatc atttttttgt atgtataaag tttcaatttt tcttcactta cgattttttt
     3361 tgatattgtt gttatataaa tgaaaggagt tttacaaatg attgatccaa aaatgttggg
     3421 gatgatcttc ctcattaatt ttgcttatat cactctgaac acgttacgtt tcatgttgac
     3481 aatgaaaggc tatcgactga ttgctccact tgtcagtatg gtcgagatca cgatttatat
     3541 cgtcggctta tcccttgttt tagatcgttt agataatcca ctgaatttat ttgtctatgc
     3601 gttaggttac gcagtaggta tcagtgtcgg aattttgatt gaagacaaat tggcattagg
     3661 atacatcatg gtcacaacga ttttgccagc aaatagcgca gaagataaaa gtttgccggg
     3721 tatactgaga gaacacggtt atggtgtgac acagacttct gcaatggggc tggaaggtga
     3781 gcggctagtt ctcgaaatac tttctcctag aaaaagcgaa cgtgaactgt atcgcctgat
     3841 caaagaaatc gaagagcgtg cttttatcat ttcttatgag ccaaaatata tttcaggtgg
     3901 tttctggact aaaaaagtga gaaaacgaca aggaaaataa ctcacaaact taaaaactgt
     3961 ctaatatcta aattaaattt taaatgttag acagttttta tttattattt tgattaaaaa
     4021 acatcgacat atactcatca atttgttatt atactttata tacttatcaa tttaatttgg
     4081 agatattatg gaaattcaac gtagaaccac taatttttta catttaggct ctttttttgc
     4141 aactttgacg atcttatttt ttctatgtta ttcatacctt aagacctcac atgcgccttc
     4201 tagtgcatta ggctggttgc ttttggatca ttctgatatc acagaagccg aagaaaatcc
     4261 gttttttttg attttgtctt ctctttgcgg cttactgttt tctgtttatt tcggtgccta
     4321 tttttttcat cgacatcctg gtacgttaaa cgaagcttca agcgtattga ttgcatttaa
     4381 aacgttttct tttttagcaa ttctttttta ttcgtttaat cacaatacgc ttttcttctt
     4441 tatcatgaat ggtttagtcg tgattttatc catttttact tattttattt tttcactcat
     4501 ttttagccaa ttgaaatgtc cgctcttctt tcgattagtt cctgtcacct gttttattta
     4561 cagcttgttt tccttttttg tccttgcttt aatgccactg accaaccctt cgacgatcca
     4621 agtcgcagga aatctgttgg atatgctctt catcgtcagt ctaggtgtat ttatgtggca
     4681 aagaaaaaac aaacaaaata aaaagcattt tgaagcctga ctattacgac ttcataaatg
     4741 cttttttagt tttaaacact tcaatctaaa agaataaccc aagattggat aaattattaa
     4801 tgactcgcca ttcgtttctt agtgattctg gattatcaaa tggacttcct aatctaatca
     4861 gctagttctt cccataaaat gccatacttg actgaatcat aatattgatt atttaaaaat
     4921 ctaacttttc ttatcactac ttcctttgtc attccacatt tctcaccatt tttttgcatt
     4981 cctttgtttc cagaccatgt agtgaatcta ataggtggta gataggcaaa atactcaaat
     5041 aattctttta accataaagg aagagaaagg ctcctcctgc aagagtcagt ataatttttg
     5101 cttactgctc tcttaggttt tactcactaa aactgttcca tattctttga tataagaaaa
     5161 tgcatgcggt ataaatttct tgctcagctc agaaaagatt tgtcgtttaa gaaaggccat
     5221 ttcctccatt gtcaatgaag gatccaatgt tggtaattca atctcaaaat tgtttttttc
     5281 gacaataggt aattctaatg tgaatgattc ttcttccttt atagtattcg tcacaagtag
     5341 agactgatgg aaaatactag gccggcgatt tttatattta ttcttacgta tcctgtcaac
     5401 gataacttct atttgatcaa aaaagtacga ttcatttacg tctccaataa gttcggctaa
     5461 ttctttgaat gccttcgtta caggaagctg attcttctgt acaaaaaagt aattagccaa
     5521 agtaagcggg aagtgttcta tagaggaaag tgtgataaaa cggtacttct catttcctgc
     5581 agttgtcctc agcaattctg tctctcggct gtttcttact ccataaaaat acgcttcttt
     5641 ccgttcatct tcgaaacaat acgcccaaat aacttctccc atcgctaatt tttgttcatc
     5701 aacagagagt ctcttcaatt gttcagctat ggtttcagca gccgaagtgg cttgttgcaa
     5761 gtaatcttta ggatcaaata ttggaaagtt caaatgatat cttcgttctt ccctcttgat
     5821 taatcctgca tcgatcaata aatcaagaaa atgggaaaat ttattttgcg aaaattcttt
     5881 tttcaattct cttagaatcg tttcttgttc tcgatgttca tatagataat cggtcaatga
     5941 agaaaaaagc ggatcatcgg tcagtttttg ataaaagttt tccttatttt ttgaataata
     6001 gttcatatat gttctctttt ctatccgttt tattttactt aaaaggcttt tacgtaaaaa
     6061 ggaggaagaa caagagtcat aattgactgt tattctcctc catttcttcg gcctttagta
     6121 tgttctatgt ttctttttat atcaatcgct tatccattca ttttgaatgc ttcatactac
     6181 catttgatta gctaaataca gccgtcaatt gaggtacgac ttgtttttta cgtgaaacga
     6241 cacctttaag caaagcacgg ttgttggtca aagttgtttg gaaagcttct tctacttttt
     6301 cttttccttt accagcaaca agtaattctg aatcactgtc taagatgttt gtgacaacta
     6361 atacgaacag atcgtagcca tttgctttca tttcatcttc cattgctgct tctagctctg
     6421 cttgtcgatc catcacttca ttaaggtcca ctgtattgac ttgagcgata cgaacgttgg
     6481 aactgcccat tgggaaactt tttgcatcca aatcaagaag aaccgcagct gatttatcac
     6541 ttagatttgt tcctgctttc aacatttcaa gtccataagt gtttagatcg acttcagcga
     6601 ttgctgcaag ttcttttgca gctgcttcat cttcaggcgt acatgttggt gatttgaaca
     6661 acaatgtatc tgaaatgata gctgagagca tgattcctgc gatttcttta ggaattgtta
     6721 cttggttttc tttatatagt tttaaaataa tcgtacttgt acatccaact ggttctgcac
     6781 gatagtacaa tggattagct gtttcaaagt ttgcaatacg atggtggtct actacagcta
     6841 aaatatttac ttcgctgata tctgaaatac tttgttggaa ttcattatga tccacaagca
     6901 tgacatctgc attttctgct ttagctgact ctactactcg tggtgcagat agctgaaagt
     6961 ggtctaatgc gtattgtgtt tcttcattag gtgtacctag agcaactgct tctgtttctt
     7021 tacctaattc attttgcaag tgtgaaaacg caatggctgc cccgattgca tctgtatctg
     7081 gattttggtg accaaaaact aaaatttttg acatgtcatt cgctcctttg tgatttctcc
     7141 tatttaataa tagcaaaaaa acggaaagaa gcaatactta tcttctttcc gatgcgacct
     7201 atcgcgttaa atattttgta tactcttctg tatgaagcaa tcgtttagca ttttcgacac
     7261 gttcttttgt aggtggttcg atcccttcta atggatattt caaaccaagt gtttcccact
     7321 tgtattttcc catcgtatga taaggaagaa tctctactct gtctacattt gacaaggttt
     7381 gaataaagtt gtctagtcgg atcaaatatt catcataatc acttcgctca ggaactagta
     7441 catggcgaat ccaaaccggt ttgttgattt ttgacaaata ttgagccatt tctaaaatat
     7501 tttcattgtc ccaatgcgtt agttttttat gctcttcatt atcgatgtgt ttgatgtcaa
     7561 ataacagtaa atctgtatac ttcatcaatt cttgaaaacg tgaaaagaaa ggttcttcat
     7621 acgtaaaagg ttttccgcat gtatccaatg tagtatgcat cttccgctct tttgcttttt
     7681 taaatagttc gatcaaaaaa tcgatctgca atagaggttc tccaccacta acagtaatac
     7741 cacctttatc tccccagtaa ggtctgaagc gttctgcttt atctaaaagt tcatctgctg
     7801 tgtattcttt ccctccgcca atattccaag tatctggatt gtgacaaaat tggcaacgca
     7861 tgcggcatcc ttgcataaac acgacaaagc gcagtccagg tccgtctact gaaccgaaag
     7921 tttcgataga atgaacatag ccgattgttt tttcttccat ttaagttcac atcttttctt
     7981 tgatttcatt ttaagtacaa gttagctgtc gaagcgaagc tttctgaaca aaatttttcg
     8041 caattgccgc ttcgttatgt gtaaaattta cagggacgga acatacaagt cctgcccctg
     8101 taaatcgttt ctttcaaata agcatactga ttattcgatt ttgatgcgat ggctattcaa
     8161 cgtcctttcg acgctaagtc gaaaaacttt tttcacgaac tttttacatt ctgtcgtgtg
     8221 aagttctcat caagacatct aattgttgtt ctcttgtcaa gtcacggaat ttaactgcat
     8281 aaccagatac ccggatcgtc aagttcggat atttcattgg attttccatt gcatcacgca
     8341 ataaatcatt actgaataca ttgacattca aatgataagc tccttttgag aagtatccat
     8401 ctaagacatt acgcaagttt tcgattcttg tatcttgttc ttttccaaga cctgaaggat
     8461 tgattgtttg ggtatttgaa attccatcca atgcacagcc gtaattcatt ttagctgttg
     8521 agttcaggct agctaataac ccactttgtt ccgcattgta acttggattt gctcctggcg
     8581 caagtggttt tccagcttca cggccatcag gagtatttcc tgtattttta ccgtaaacaa
     8641 catttgaagt aatcgtcaat aatgaagttg ttggttttga gttgcggtaa gttttatgac
     8701 gtttgatttg tcccatgaag tattccaaga tccatgaaac taattcatct gcacggtcat
     8761 catcatttcc gtatttaggg aagtcgccat ccacttcaaa atcaactgct attccttttt
     8821 cattacggat tgcttttact tttgcatatt tgatcgctga caagctgtct gccgcatgag
     8881 agatcccagc aatacctgta gcaaatgtgc gttgcaagtc agaatccatc aaagccagct
     8941 gagctgcttc ataagaatat ttatcatgca tataatgaat gatgttcaac gtattgacat
     9001 atagttctgc taaccaatcc atcatattca tgaagttatg tttcacttct tcaaagtcta
     9061 gtatttctga tgtgattgga cgatattctg gtccaacttg ggcgccagtc acttcatcga
     9121 ctccgccatt gatggcatat aatagtgttt tagccaagtt agcacgagcg ccaaagaatt
     9181 gcatgtcttt tccgacaact gttcctgata cacagcaagc aatcgcacaa tcatcagaac
     9241 cccattgttc acgaagctga ttatcatttt cgaattgaat cgaagaactt tcgatagcga
     9301 ttctgctagc atatgttctg aatccttctg gaaggtgttc agaatacaaa acagtcaagt
     9361 ttggttctgg tgaaggtccc atatttgtta atgtatgaag cagacggaaa tcattctttg
     9421 taactaatga acgaccatct aatcccatac ctgcaatgga taaagtagcc cagataggga
     9481 atcctgagaa taattggtta tattcaggtg tacgcgcgaa tttgaccata cgtaatttca
     9541 ttactaagtg gtcgatcaat tcttgtgctt ctttttctgt gattactcca ttatccaaat
     9601 cacgttggat atagatatca aggaatgctg agatacgtcc gattgacatc gcagcaccat
     9661 tttgtgattt gattgctgct aaatatccga agtatagcca ttggacagct tcttttgcat
     9721 ttgcagctgg tttagaaata tcatagccat atttcgcagc catttctttc atttgcgcta
     9781 atgcacgata ttgttcagtg atttcttcac gtaaacgaat cacttcttca ctcattgtat
     9841 gataaccagt catatcatga tcttttttct tttgttccat caaatagtcg attccataca
     9901 atgccacacg acgatagtca ccgatgatac gtccgcgacc gtaagcgtct ggaagccctg
     9961 taatgatttt gtttttacga gcagcacgca tttcagcagt ataagcgtca aatacacctt
    10021 gattatgtgt tttgcgccag tcagtgaaaa tatgtgtcaa ttcatcgttt ggtgtatagc
    10081 cattgctttt tagcgcattg ttagccatat tgattccgcc gaacggcatg aatgcttgtt
    10141 tcaaaggttt gtctgtctgt agaccgacga ttttttccaa atctttatcc aaatagcctg
    10201 catcgtgtga agtaatagtt gatacgattt ctgtgtccat atcatagaca ccgttacgct
    10261 cgaattggat atcatttaac ttttgcaatt catcccataa tttttctgtt gcttctgttg
    10321 ggccttccaa aaagctgtca tcaccgcgat attcagtgta gttattttga ataaagtctc
    10381 tagtgtctac gccttctttc catttggtac ctttaaatcc attccattgc tccattgtat
    10441 ggacctccta aatttctgtt ttatgtaaca gtttttacgt gattagtata acacagcacc
    10501 ctatctttgc aagggttttc ttgtttttta tttaacaatt tttatgtttt atttacatat
    10561 atttaaatca acgttgacat accaacattt aagcattgtg aacactttca tctttttttg
    10621 caaacgtata aggatttatt aaaaatgaat gaaaaagaga cgccacctgt attaatacag
    10681 atggcgtctc ttcattaaaa tactacaaca gttacgactc gatgaaaatc tcaggagcat
    10741 cgataacttc gatgacttct ccaccttttt tctcatccat cgcaaatgat ccattcgatg
    10801 ttcgatcacc tatagggaaa tcagttgggt caatcgttaa ctgctgccct ttttgattca
    10861 tcaaggttat ttcttttgat cttccttccg acatatagat aatacggtgc ggattttttt
    10921 tcagctcacg catgaccgtc actccccgct tcgcacgtga agtttggctg atttcttgag
    10981 caagcatgcg tttaacagct cctcgctgag taacgattac gatcggcgta tcaccttctg
    11041 ctaacacaag cagcccgttg ataatcctat ctccatcttt caaattcata gacttcactc
    11101 cagctgcctt tgctccaaca acgggaacat ctgttaaagg atagcgcaaa cccattcctt
    11161 gatgcgttac taagaaaaca tctaagcctg gctgatcttc tttcagatac acagcaatca
    11221 gttcatcttc ttccgccttt agcttcatac agctgagcgg acggctctta taggtgcgcc
    11281 atggttcgaa ctctgtcatt cgtgtttgct tgatcaatcc attcttgctg atgaatacaa
    11341 atgttttctc tgcttgaatt ttttgatatg ggaaaacagc taaaatcgat tcgtctacag
    11401 acagattgga gattgtttga gaaatgtgct caccagcatc tttccatttc aaatcgggta
    11461 gttcatggac agggcggtag atcacatttg ctttatttgt gatcagaaga acatggtcca
    11521 atgtactcaa ttcacctgag tatagcaagt agtcgccttc tcgcatcccg atttcttctg
    11581 gtttggaagc actataagaa cgaaggcttg ttcgcttcac atagccttct cgcgtcacac
    11641 tgacaattac gtcttcttgt gccactaaga cttgcgtatc aattttgatt tcttcgattt
    11701 cgtcttcgat tgtcgttaga cgagcggaag aataattttt tttcacttct cgcaattctt
    11761 ttttcataac agagaaaagc tctttgtcat ttgaaaggat tttattcaac tctgtgattt
    11821 gcgcaatcaa gctttcggat tccttttgaa gctctgtgat atccgtgttc gtcaaacggt
    11881 ataattgcaa tgtgacaatt gcttctgctt gttcttctgt aaattgaaag acgtcgacta
    11941 aattcttttt ggcgtctttt ttgtctttac tttcacgaat cgttgcgatt acttcatcca
    12001 gaatcgataa agctttcatc aatccttcta cgatatgctg tcttttttga gctttggcta
    12061 aatcgaattg actacgtttt gttatgactt gctttcgatg ctcgatataa cttcttaaaa
    12121 tgtctttcag tccaacttga tgcggagtca tatggtcgat agcgaccata ttgaaattgt
    12181 aattgatctg tagctcagtg tttttgaaca aataattcaa aattccttga gcattggcgt
    12241 cttttttcaa ttcgacgaca atttgcagac ctgttctatc actttcatct cgtacttcag
    12301 ctattccatc gattttctta ttcaaacgga tctcatccat ttttttcacc aaggtcgctt
    12361 tatttacttc atatgggatt tctgtgatca cgatttgctg tttattaccc ttcattgcct
    12421 ctatctttgt tttcgaacga agaataactt ttcctcgtcc tgtttcataa gcttttttga
    12481 tttcatcttt tccttgcaag atccctcctg ttggaaagtc aggtccagga atatattcca
    12541 tcaatttttc aaggctcgct tgtggatgat cgatcatata gattgtgcca tcgatcactt
    12601 cagctaagtt atgagtcggg atttctgttg catatccagc agatatacct gtcgatccat
    12661 tgacgagaag gtttgggtat cgagctggaa ggactgttgg ttctttttct gtatcatcaa
    12721 aattccacac gagatcgact gtttctttct cgatatctgc tagcatctct ccacttaact
    12781 tggataatct ggcttcagta taacgcatcg cagcaggtgg atcaccgtcc atactcccgt
    12841 tattcccgtg catttcgatc agtacttccc gcaacttcca gtcttggctc atgcggacca
    12901 tcgcttcata aatactgatg tcaccatgag gatgataatt ccccatgata tttccgacag
    12961 attttgctga tttacggaat cctttgtcat acgtattgcc atctttgttc atggcaaata
    13021 atatccggcg ttgcactggc ttcaatccat cacggatatc aggcagtgca cgttcttgga
    13081 taatatattt ggaataacgt ccgaagcggt cacccatcac ttcttctaaa gttaattctt
    13141 gtacttcttg tcgattttcc atcaggtgac ctcctattct acttcaaata actgattgtt
    13201 ttcattttta tctgctcgtt cttcatctaa taaatcatta gaaactgaag gagagatttc
    13261 agtatcctct tttttatcta agatactgcc atcttcttcg agcgtaaatt gcacgtgatt
    13321 ttcgatccat tttcttcgag gttctacttt gtcacccatc agtgtggtta ctcgacgttc
    13381 agcttgcgca gcatcatcga tacggacgcg gatcaacgta cgggaagtcg gatccatcgt
    13441 tgtttcccac aattgttccg cattcatctc tccaagtcct ttatatcgct gcagcatata
    13501 tcctttgccg acttttttaa tcattgctgc caattcgtca tctgtccacg catattcaat
    13561 gacttgcttc tttccttgtc ctttggatac tttatataaa ggcggtaacg cgatatatac
    13621 tttcccagct tcaatcagcg gtttcatata acgataaaag aaagtaagca gtaatacttg
    13681 aatatgggcg ccgtctgtat ccgcatcggt catgatgatt actttgtcgt aattacagtc
    13741 ttcgatagaa aactcaggac cgactcctgc accaatcgta tagatcatcg tattgatttc
    13801 ttcatttttc aagatatctt gcattttagc tttttcggta ttgatgactt ttcctcgtaa
    13861 aggtagaatt gcttggaact ttctgtctct tccttgttta gcagatccgc cagcagaatc
    13921 cccttcgact aaatacaatt catttttctt agggtttcgg gattgtgcgg gtgtcaattt
    13981 tcctgaaaga agagattcgc cttttttacg cttcttccca tttcggcttt cttctctggc
    14041 tttacgtgct gcttctctcg cttctctggc tttgattgct ttacggacca acatctggct
    14101 catttcgcta ttttcctgca aataaaagcc catttgttca ctgatgacgt tatctactac
    14161 ggtacgagca actggcgtac caagtttttc ttttgtttgc ccttcaaatt gcagaagatt
    14221 ttcaggtaca cggatagaaa gtacagcagc taacccttct ctgaaatcac taccttccag
    14281 attcttgtcc cgttctttca gcaaaccaac ttttctcgca tattcattat aagatttcgt
    14341 catcgccgct ttcataccag cttcatgggt tcccccatct tttgtccgaa cgttattgac
    14401 aaaagataaa acattttctg aataaccgtc attgtattga taagcgacct ctacttcgat
    14461 accttctttt tctcctgaaa aataaacaac tggtgtcaat gtatcttttt cttcattcag
    14521 ataatcaaca aattctttga ttccttcttc gtaatgaaaa atttccttga ctggttcttc
    14581 tccacgaaga tcagaaagag agattttaac gccttttaga agaaaggcag attctcttaa
    14641 ccgttcggct aaaatttcat atgaaaattt cgttgtactg aatattgtat catctggtaa
    14701 aaaatggaca gtcgtcccgt ttgctttttt tgtttttcct atctttttca aagtaccgtc
    14761 aggtttaccg ccgtttttaa atttctgctg atactctacg ccatctctaa cgatcgttac
    14821 aattagccat ttcgataagg catttaccac actggctcct acaccgtgca gtccaccaga
    14881 agttttgtac cctccttgac caaactttcc gccagcatgc agcacagtaa aaatcacttc
    14941 aactgttggt attccggaag catgcattcc tacaggcatc ccacgtccgc tatctgcaac
    15001 agttatacta ttgtcttcat ggatggtcac atcaatctca ctgccataac ctgataatgc
    15061 ttcgtcaact gcattatcga cgatttcata gactaaatga tgcaagcctc ggctgtcagt
    15121 ggatccaatg tacatccctg gtctttttct aactgcctct aatccttcca atacttggat
    15181 ggacgagtca ttgtattcgt tgtttacttt ttttgccaag gcaaacgctc cttcaataat
    15241 actttgcggc aaaaccgcac cacgattatc atactagaaa aggtcgtgaa aaaaaagagg
    15301 gtagacgaga aatcaatttt aattatctcc taaccgcttt ttcgacattc ccctctatcg
    15361 atcatctctt ctttgtggtc atttttattt ttggttttct ttcaacttac caagttcaat
    15421 cttaatacaa cgatccatta cgatatctgt acgtcctgct tttcttagca tctcagctgc
    15481 ttctttgttt tcaatcccta attgtgccca gaacacttta gcatcagttt tcaaaaaatc
    15541 acctgctatt tcaggtaaaa attcactccg tctaaataca tctacgatat cgattggatc
    15601 atcgatttct gtcaaattcg catagacttt ctcgcctaga atttcttcac cagccaataa
    15661 tggatttact ggtatgattt tatatccatt gtcttgcaat acctgtgcaa ccatgtaact
    15721 tgttcgttct ggtttattgc tcaagcctac gacggcaata ttttttgctt cttccaaata
    15781 gttgaaaatc gtttcttgtg ttggattttt tacagacatt tctgccaccc cttgcttttc
    15841 tttttattcc atctattata cttgtatctt tcattttttc aattgttcct gttacctatt
    15901 catgctaaaa taaaggatag ttttcaactt gaaagagggt aaaaaatgaa attgatctta
    15961 ttacttattt tagcatatct tttaggttct attccttccg gtgtctggat tggcaaattg
    16021 tttttcaaaa aagatatccg acaacacggg agcggtaata ctggaacgac caatacattt
    16081 cgtgtattag gcaaaacagc cgggattgtt gtcctgctta tggatatttt aaaaggtaca
    16141 ttggctactt gtttgccgat gatcttccac ctttctggga ttaaccctct ttggtttggg
    16201 gtatgcgcca ttcttggcca tacatttccg atttttgctg ggttcaaagg aggaaaagct
    16261 gttgcgacaa gtgcaggaat gcttttagga tttaatcctg tattttttgt ttattcttgt
    16321 atcatcttta tcgttagttt attttgtaca agtatggtga gtttgagcag tatgatcagt
    16381 gcagtattga tcactttatc tacgatcgtc cttccttatg tagctcctgt gattttggct
    16441 aagcccaatt ggttactgac gatcattgcc tttttagttt cttcctttat cttttatcgg
    16501 cataaagaca atatcaagcg tatcagaaac catactgaaa gtcgaatccc gtttggtttg
    16561 aatagcagca agaaaaaatc ctgatacttt ttcattccaa agagataaaa cgggagtgtg
    16621 acaaatcgat gtcacactcc cttattactt acaggatagc ttttttacac accttggtaa
    16681 acggtgttcc ctgctaactt ctcggtcaca acctcttact tcactgtgat ggaatatttt
    16741 gtcttgaaga tttcatgggc acctagttga ttgatcccta ctttttccat caagtttcca
    16801 ctactgttca ccgtgtcagc gattccgcac cacggttcga tacaaacgaa aggcgcttct
    16861 tttggataag gagaccagat ccctacataa ggcatatctt tatagcttaa agttacgcta
    16921 tgggaagatt tttcacttcg aatggcgtac gcatttagac cccttgtttc aaaaaccaag
    16981 gcatcttgct caaaaagttc acgattcaaa gccagattcg tatttgtctg acctaacgtc
    17041 ttttgatcca tatcgatgaa tgggcctttc aatggtaggc gtaatcttga tttcaatgga
    17101 gaaaatgcca gatagaaatc ttcaaatgac aatccttctt caagaggtac attgaaagca
    17161 ggatgtccac cgatagaaaa gaacatttct ttttcaccgg tatttgccac ttgataatga
    17221 accgtgatgc cgtctcctcc taattcatag ctgatcacta attcaaaatc aaatggatac
    17281 ttttctaatg tctccggcgt actccttaag atgaaactag ccatttcttg tccatgttca
    17341 atgacttcaa aatccatatc ccgagcaaat ccgtgttgcc ccatttgata tgttttccct
    17401 tgataaacat actgatcatc ttttaaacgt ccaacaaaag ggaataaaac cggtgcatgt
    17461 ctgccccaat acgctggatc accttgccag atatactcta aaccatgttc ttttgatttc
    17521 agactgatca attccgcacc gtgttcagcg atttcggcga tcagctggtc attttgaata
    17581 ataacagtca ttgccattcc tccaacttcg acttactttt taaaatggga aggctggaac
    17641 aaacatttct atctgcccca gtccccttac ttctaataac tgtgttttgc taactcgtct
    17701 ttaaaacggg aattcaatac ttttaaataa gttcccttca tgcctaacga acgagattca
    17761 atgattccag ctgattccaa ttttcgtaaa gcattgacga tcacagaacg agtgatgccg
    17821 atactatcgg caatattaga agcagtcaga cgaccttcat ctccatccaa tgcttcaaag
    17881 atagcttgta cagccttcaa ttcgctataa gataatgtgt tgattgccat ttggacagcc
    17941 gttgcactcc gtacatctgc ttcgatgctt cgagacttct gataaaggat ctgcataccg
    18001 acaaccgtag ctccatattc tgctaagact agatcgtcat ctgcaaacga ttgttccata
    18061 cgagataaga taatcgtacc caaacgttcg ccagctccaa agatcggaac gacagtcgtc
    18121 agaccaaatg gatatttctc tcttgactct accggaaatg cagtaatatc actagtaatt
    18181 gggatattag cttcagtttt catcaactgt gccactgtat cagtataaga ttcaggaaat
    18241 tgtttctcaa caaacattgt tttgattcgt tcattgttca catcgtgacg ttcattataa
    18301 cctagtaata ctccgtcact tcctatgata taggtgttgc tgtcaagaat accacctagt
    18361 gtgacagcca ttttatcgta aggcaaatct gcttgcatgt caaaagtatt cttttgctgc
    18421 aaaagtgtgt ttatcttgcg ggttttcgtt agcaagtctg tcattgtcgt cctccgattt
    18481 tttataaaat atagcgactt aaatcttcat ttgccgcaat tgagccaagt ttgtcatcca
    18541 catatgcttc tgtgatcgtt atttctccca tttgcatatc tggtgcttca aataatagat
    18601 cttctagtag tttttccaaa atggtatgca gtctgcgtgc gccgatatta tctgtgtcac
    18661 ggttgacatc gaaagcgatt tgagcgatcc gttcaattgc ttcttttgta aacgtaactg
    18721 acacattttc tgtaccgatc aacgcgacgt attgttttac taaagcatta tttggttcgg
    18781 tcagaatctt cacaaaatct tcagctgtca aatcatctaa ttctacccgt atcgggaagc
    18841 gtccttgaag ttctggaatt agatcacttg gtttagacaa atggaaagct cctgaagcaa
    18901 tgaataggac gtgatctgtt tggatgatcc cgtattttgt gtttacttgt gaaccttcga
    18961 caattggcaa tatgtctctt tggacgcctt ctctggatac ttctccagat tgctgtgatt
    19021 tagaagtgat cttatcgatt tcatcgataa aaataattcc actttcttcc gctaatttga
    19081 ttgcctcact atggatatca gcatccttga ctaattttgc tgattcttcc ttgatcaata
    19141 actctaaagc ttctttgact gttaccgtcc gttccacttt tttcttcggt gtcaaagcac
    19201 ctagagtttc atttaggtcg atccccattt gctccaatcc attgttcatc attggtgcag
    19261 gtttcttttg gtcttcaact tcaatggtta cttcacgact atccagtaaa cccttttgta
    19321 gttgttccaa gactgtacgg cgattgattc gaatatcttc tgtcacttct tcttgcggtt
    19381 cagcggtatt ttgctgacta gcattaaaaa gattcatcat ttgttcaaat tgattagtgg
    19441 attgtttctg ttcttttttg ataccaggta ccaagatttt taccaaacgt ttttctgcat
    19501 tttttttagc acttgtgtat acacggctgt actgctcttt ttctacgatt tggatcgcat
    19561 tttctactaa atcccgaacc attgattcta catctcttcc gacataaccg acttcagtaa
    19621 attttgttgc ttcgactttg acaaatggtg cattgacgat ttttgccaaa cgtcgtgcga
    19681 tttctgtttt accgacccct gtaggtccga tcatcagcat attttttggt gtaatatctt
    19741 gttgcatttt ttcatctaac tgcaaacggc gatagcggtt acgcaaagca acggcaacag
    19801 atttttttgc tgcgtgctgt cctacgatat attcatctaa ttcacttacg atttgtcttg
    19861 gtgtttgttt taattcatac atatctactc acctcttaca gttcttctac gataatatta
    19921 tgattagtaa agatacagat atctgcggca atatttaacg cattttctgc aatttctttc
    19981 gcactcatgt cgttttgagc atagtttttc attgctcggg ctgcagccaa cgcaaaattc
    20041 cctcctgatc cgatagctaa gataccatca tctggtgtga taacttctcc tgtacctgaa
    20101 acaagaagca tttctttatc attcattacg atcagcatcg cttcaagttt ttgcatagat
    20161 tgctgtgtcc gccattcttg agctaattcg acagctgcgc gttgtagatt cccgttatat
    20221 tcgtttagtt tgccttcaaa tttttcttct aaagtaaacg catctgcaac acttccggcg
    20281 aaacctacaa caacttctcc attgtagata cgtcgtactt tctttgcagt gcctttcatg
    20341 acaacagatt cacccattgt tacttggcca tctccagcca tagcaaattt cccgtctttt
    20401 tcgacggcac agatagtggt tgaatgaaat ggtgattcga ccatgtttgt ttcctcctaa
    20461 atttgttctt ttccttattt ttacgctctt ggatggaaag aacgatagtt tttttgtaaa
    20521 ctttcttttg ttacatgggc atagatctgg gtagtcgata aattggcatg tcctagcaat
    20581 tcttggactg tccgcaaatc tgccccatta tttaataaat gagtagcaaa tgtatggcgc
    20641 agcatgtgag gatggatatc actatccaaa ctgctttttt tgatcatttg attcaagaca
    20701 tattcaattc cagtaggcgt gattggtttt ccatgatgat tcacaaaaac gatatcatgc
    20761 tcttcgtgat attttgtcat caatacctgg cggccttccc tgaaatattc ttgcagagca
    20821 tcttgagcaa aagaacctag aggagcatat ctctctttgt tccctttacc atggatcaac
    20881 atgacctctg aatcaaaatc aatgtctgat accgacaaac tgctacactc acttaaacgg
    20941 atgcctgacc cgtaaaggac ttccagtaaa gcgcggttcc tcaaatccaa aggactatct
    21001 cctgctacgc tgtcaaaaag tgcttgcatt tcattttcat aaaaaaaacg tggcagtctc
    21061 aaatttttct ttttgagatg aacgtaagaa aatggatttt cttttatcac ttcttctttg
    21121 agaagatact gataaaacga acgtaggctg gctatcttgc gactgataga atttctgcta
    21181 tagctaggac gatgatcagc taatgcactc aaatagacac gaaccgtaaa atattccgcc
    21241 ttcagcagat cagcgtctcc agattcctcc aaaaaatgtt tgaaatggag aatgtcttct
    21301 tcatatgctt ctttcgtttt ttctgaatat cctctttcga caatcaaata acgcaaaaat
    21361 ttttgttccc acgtggcttc tgtcattttc tcaccttcct tttgtcccag agaataattt
    21421 agcacagcgt ttataaaaag acaaataaat tgtcagaatt taaccaatat ttctaagttt
    21481 gttcacaatt ggcgcaattc ctcacatttt gtttgagaaa ttcttaagaa aaacgcaaga
    21541 aaaagagcga gactatccgt ctcaccctct tcatggagtt tccttactca tttgggattg
    21601 cagttttttc taattctgtt tttattgcct cattagcttt taaagctcgt tgtgcaattg
    21661 cttcatatct tgcttttttg tctcgaatgc gctctggcag ttcaggaaac agaccatagt
    21721 tggcattcat cggttggaaa tgttttcctt ccgcatgagt aatgtagtaa gccatacttc
    21781 caatagctgt ttcttgtgga aactcgactg gttccatttc ttttgctaag cgtgcggcgt
    21841 tgattccagc aagtagtccg ctcgctgcac tttcaacata gccttctaca ccagtcatct
    21901 gacctgcaaa gaacagatct tcgcgtttcg ttgattggta agtcggtttc agcaattctg
    21961 gcgaattcat aaagctgttt cggtgcatca caccatagcg aacgaattca gcattttcta
    22021 acccaggaat catccggaaa acacgttttt gctcgcccca tttcaagtga gtctgaaagc
    22081 ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg cagctggata acagcataag
    22141 gacgtttgcc agttttagga tcttccaatc caactggttt cataggtccg aaaagcatcg
    22201 ttttgaatcc tcgctgtgcc attacttcaa ttggcataca tccttcaaaa tatttttctt
    22261 tctcaaaaga tttaagttct gccacttcgg cattgattag tgcctcatgg aaagctttga
    22321 attcctcttc tgtcatcgga cagttcaagt aagctgcttc tcctttgtca tagcgggact
    22381 tcaaatatac tttatccata tcgatcgtag ctttgtctac gattggtgca gctgcatcat
    22441 agaaataaaa accatctgaa ccattgaatg ctttgatgct ttctgctaat ggatgagaag
    22501 tcaaaggtcc tgttgctact acggtgattc cttccggtat ttcagtaatt tcttcgttta
    22561 tcactgttac taatggatgc tctttgactt ttcgggtaat tgtttcagaa aaagaatcgc
    22621 ggtcaacagc taaggcacca ccagctggca ctgctgtttc atctgccgaa gtgattacta
    22681 ccgaattcaa tcggcgcatt tcttctttca ataaccctac tgcattagcc aaactgtttc
    22741 ctcttaatga attggaacat actagttcag caaaattact tgtgtgatgc gcctcagtcg
    22801 atttgactgg acgcatttca tataaacgga cagggactcc tgcttgtgcg atctgccaag
    22861 cagcttcact tccggcaagc cctgctccaa caacatttac cattttcaat gaaagaacct
    22921 cttttctatt atttttggat gtcttcttcg taatctccat ttacacagac tacttgttta
    22981 ccgcctttga ctttcttctc gaccagatat ccgtcacatt ttggacaatt tctgcccact
    23041 ggtttatccc aagaagtgaa ttcacattct gggaaacggg aacaaccata gaatagtctg
    23101 tttttcttag attttcgttc aatgacttgt ccttcatgac atactgggca agtcacaccg
    23161 atttctttta cgattggttt cgtattccga caatcaggaa aattactgca agcataaaac
    23221 ttcccatatt tccctaattt tatcaccatc ggatgtccac agacatcaca atcgaatcct
    23281 gctggttcat ctttgatttg aattttttcg attttttctt ccgcattcgt cagttctttt
    23341 tcaaacggtt tatagaaacg atcaaccact ttgacccacg cttccgtgcc ttcttcaatt
    23401 ttatccaaat ctccttccat ttctgctgtg aaatggatat cgacgatttg agggaagaat
    23461 tcacagatta atgaattgac aatttctcct agttcagtcg gctcaaatct tttttgtgtc
    23521 aattttacat agtagcgtct ttggatcgta tcaagtgttg gtgcataagt ggaaggtcgc
    23581 cctaccccgt tttcttctag tgcacgaatc aacgtagcct cactaaaacg tgctggtggt
    23641 tgagtaaaat gttgttttgg ttcaatatca acagattgga ctacatcgcc ttcttcaagt
    23701 tctggaagaa tattttcttt ctcttctttg ccgtcatctc taccttccac atacacttgc
    23761 atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga agatgacacc attttgttct
    23821 aatgtcactt tcattgtatc taaaatagct ggtgtcatct gactagccac aaaacgtgac
    23881 cagatcaatg tataaagttt caattgatct ttatctaaat attgtttgag ttcatctggt
    23941 gtacgaagca cactcgttgg acgcacagct tcatgggcat cttgtgcgcc ttgagcattt
    24001 ttgatctttc ggtgatcatg tctagaaaat tcttttccat acgtatcttc aataaatgct
    24061 gcggcctctg cttttgctgt atcggcaatc cgggtagaat cagtacgcat gtatgtaatc
    24121 aagccgactg ttccttgttt acccaaagtg attccttcat ataattgctg tgctaccatc
    24181 atggtttttc ttgttcgaaa gtttaactta cgggcagctt cttgttgcag gctacttgta
    24241 gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt ctactttttt aacttcgaag
    24301 tctgttccat caattctagc agtgacttct tttacacttt gtgcatctgg taatttcttt
    24361 ttcttaccgt cgattcccca gaaatttgct ttgaatttct tgcgagcttt tttaaaatta
    24421 ccgtcgatgc tccaatattc ttctggaaca aattctcgga tctctttttc acggtcaata
    24481 ataattttta aagcgacaga ttggacacgt cctgcactta accctttctt gaccttacgc
    24541 cagagaatag gactgatcga ataaccaacg attctgtcta aggtacgacg agcttgctgt
    24601 gcatctacta aatctacatc gatcgttctt ggttccttaa aagctgcttt gactgcgtct
    24661 tttgtgattt cattgaagac cacccgattt ttttctttca gatccaaccc aagtaggtac
    24721 gctaaatgcc aagcaatcgc ttctccttct ctatccgggt cacttgccaa gtaaactttt
    24781 tgagcttttt ttgctgcggc tttcaggctt ttgatgacat cgccttttcc gcgtatagaa
    24841 atataatggg gttcataatt gttttcaaaa tcgataccca ttttactttt cggtaaatct
    24901 cttatatggc caacactggc cattacttta tagtttcgtc caagatattt ctcaattgtc
    24961 ttggcttttg caggtgattc tacaatcact aaatatttat acgccattac tgaatggact
    25021 cctttccatt gtcgtgttcc tactattaaa agaggattca tacaaatcgt agctaatcat
    25081 atccattcag aaaacgtttg tcaagaaaag caaatgaaaa gtatggtgtt attctttaaa
    25141 ttctgccaag atatcttcac ccgaaaccgt gcaaactgca ccgtcttgga tcaaatgatg
    25201 acatccggta gaatgttctg ttaaaatatt tcctggaaca gaaaaaactg ttcttccaag
    25261 atccaatgct tgctgtgctg ttatcagaga gccgcttttt ttcttggctt caaagacaca
    25321 tgttccttta gacaaagctg cgataatccg atttctcatt ggaaaacaat gctttttcgg
    25381 gccggattga ggaggatatt cactcaaaag aagatgatca tttgcaatca cctcttgcaa
    25441 gtcacgattt tcagcaggat agacttgctc tatccctgtt ccaatgactg cgattgtccg
    25501 tccaccatga gagattgctg cttggtgacc gtatgtatct atccctttcg cgcagccact
    25561 gacgatcaca tatccagctt ctatcactgg ttttagtaaa taataaactg tttttttggc
    25621 taaagcagaa gtgtgtcttc caccaacaac gcttaacatg ttacaagaaa gtaaagatag
    25681 atcaccatta taaaacagta caattggcgg ataggtcaag tgaagcagct gagggggata
    25741 tttgggatca aagcaagtga taaacgactg tttcgattgg attttgcccc agttagcgtg
    25801 aatttcctgc caacttttgc gaaacagctc ccggtgtttg gttattttac ctatcttaat
    25861 gatgtcttca aatccgattt ctttataagg atactgcatc atccagttag caagtgccca
    25921 ttttccttga ctactgattc cttttgtata agcaagtttc aataaatttt cttctatttg
    25981 atacatcata aaaccgcctt tcgtctctct ttctaataag atacgtaaaa gcggctgttt
    26041 tttattgtaa atattttttt acaggtgcaa aacttttacg atggattggc gtaattcctt
    26101 gtcgttcaag tccttctaag tgttcctttg ttccataacc tgcattatgc gagaaaccat
    26161 atccaggata aagcacgtca tattctttca tcaattggtc tcgatatact ttagcaatga
    26221 tacttgcagc agcaatcgac acagatcgtg catccccttt gattattttt tcctgctgaa
    26281 taggcaaatc caaggtcatc gcatcgatca acaaatagct tggtacaatg gacaattttt
    26341 ctactgcttg gatcatcgct aatttacttg cttcataaat attcacatca tcgattgtct
    26401 gctgatcaac gatcccgatc ccgattgcca gggctttttc ttgaacttgt tgataaagaa
    26461 gttctcgctt tttctctgat aattgtttgg agtcattcag ccctaatatg atctcatttt
    26521 tcggcaagat aactgcagct gccacgacag gtccagctaa tggtcctctt cctacttcat
    26581 caatgccggc aatccattgg tgaccttgtg cgtacccttg cttttcaaag caaaacattt
    26641 cttcatattt ttccttaatt aatgcttcct ttttcaatcg tcgttcaaaa gaagccagtg
    26701 cttgctgcac gcctttgcgt ggatcatttc ttaatgattc tagtgtagga tcatctattc
    26761 tctgtatgct tttcaatcgt tctttgatac tttggatgga ttcagtcatc acttttttgc
    26821 tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt ttccttgacg aatatcatgc
    26881 acgactaatt cgctgccacg atcatagtct tctttatacc cacgttttgc tgtaatatcc
    26941 atcagtaatt ccggtgccaa tttttcttct tgctcgattg ataaaccata acgatcgctg
    27001 attcttcctg aataatatcg ggcaaaaaag tctaatccgt aaatcgctaa atcatctaaa
    27061 tggacaagct ggtctttgat cgcgcctgtc agcgctagtt ttttgccgat ttcttcatct
    27121 tcaaatttgg gccataaaat ccctggtgtg tctaacaatt ccaactcatt acctaaacga
    27181 agccactgct gaccttttgt tacgccaggc ttatttcctg tctgtgcaat tttcttgcct
    27241 actagtcgat tcatcaacgt agatttccca acatttggaa tacctaagac cattgcccga
    27301 atagcgcgag gcttgatccc ccgagcagca tctcttgccc gtttttcttt caatgcttct
    27361 ttagccgcag gtactagacc tttaattcct ttactttctt gtgccgtgat agctaatgca
    27421 agatggcctt gtttacgaaa atattcttgc cacagctttg tctgttgtgg atctgccaga
    27481 tccgctttat tcaataaaac aaggcgaggt ttttgctgaa tgatctgatc tagcattgga
    27541 ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa atacgatgtc cacatatttc
    27601 attttttcag aaacttctcg tcgggctttt gccatatgtc ccggaaacca ttggatggtc
    27661 attgttctct ccattccttt cataccggaa tattccccgg ctttacttgt ttttttcttt
    27721 ttcaatataa attcactttt ttattataaa gaaattccat ctattagtta agtgaaagtg
    27781 aaccagtcct cattctatca catcttcact tttttgatga tgcaaatttt tattttggtt
    27841 tctatatatg aatctaaatt tttgacagcg tttcctaaat aagagatgat agacgaacaa
    27901 aacagaaagg agacgaaaca aatgaatagt atggtttttg ttaattttcc tgttgctgat
    27961 gtcaatcgtt ccgttgcttt ttatgaaaag ctgggtttta aaaaaaatga agaattttct
    28021 actgaagaag ctggttcaat ggtatgggat gacacttttt ggataatgct tctctctcat
    28081 gatttttata aaaaatttat aaaagataaa gaaatagctg ataataaaaa aacaagtggt
    28141 gctttgattt cgtttagtgt tgaaaatgcg gatgaagtaa aaaaatttgc tcaaattgca
    28201 aaagaaaatg gtggtacttt ccacgaagta gatatgggaa tgcctgaaga agaaatgttt
    28261 tcattagaag tccaagatct agacggaaat acattagaac ccgtatggat gaagatgtaa
    28321 gcagataact aatctgacgt tttttataac aaaaaaacaa aggcggatga tacgataagt
    28381 attctccgcc tctatttttt ttattcgtac aatctctttt ttacagttta taaaagcagt
    28441 cttctcaccc tttttttaat tgttagatgc cagtgtatgt aaatcaccaa tgtgtcatga
    28501 cacattggtg attttatggt atccttttat tattaactga caaaggagag aatattttga
    28561 acactttgat tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga ttgttgtcga
    28621 cctctgctct tttattagcc cttgatgtca tcttatacaa gatagcgatt ggtcctgctt
    28681 attttcaaat cacattcgga tttttaagta tggcgcttat gggttatcta tatggaccga
    28741 tctgggctgg tttgcttgaa atgttgtcga acactttaaa tttcacgata tttggttcag
    28801 gaacgtttca agctgctttt cttatcacgg ctttccttgg tggatttatt aatggcttat
    28861 ttttatatca taaaaagata aacctgcctt ccgtcatcgc tgcccaatta ttgattatga
    28921 tcccagtcag cctttttatc aattcttggc tgatgtctgt tttgtttggt gcagatttca
    28981 aagtaatttt tctaggtaga ttcatcagga atatgatcca aatccctatt caaataatgg
    29041 ttgtttattc atttatcaaa actttagagg ctagaaaagt gtttaaacga gctgtttgat
    29101 tttacgtact aaggtagtaa actgattttg gattaaacaa taaagcgtgc agtctcaaca
    29161 gactgcacgc tttattgttt ctcactggat ccattttatt tcatcaagtg gataatagac
    29221 aaatcttgct ttacctagaa tatcagtttt attgatcgtg ccaaacgaac gactatcttt
    29281 tgaaactctt cgattatctc caagtacaaa ataattatct tccggaagct tggattgtcc
    29341 cgttagctct tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat ctgatttcag
    29401 gtttttagtt agataaggtt ccttaactac tttcccattg atcttcaatt gatcattttg
    29461 gtagctgaca ttttctcctg gaagaccgat tactcgtttg atataaatcg tgccatctgc
    29521 taattgaaaa acaacaatat caaatcgtct gatctccgaa aatttttcca tcacaaccat
    29581 atcaccttgc ttcaacacgt tttccattga attgccttcc actggtaccg ggatgaataa
    29641 aaatccccgc aacatgaacg caaggaatac agaaacaagt aagtatttga atacaagcca
    29701 gaagcgatta atatagcgtt gtttttttgt caatctctat caatccatcc ctcttttttt
    29761 cattctttct ttattataag aaaaatatca aaaaaatgcg actagatagc tgaaataaaa
    29821 ataattcttt caattaggct attggacaaa aaactttttt gatcataaag tacaaaacta
    29881 tctcttatat gtcatgatta ttttctaaat atttttccca tgcttcgtca aagatatcca
    29941 tggacggcaa atagtcgaca ttcagttcca gataattaga aatttcttcg tagctagagc
    30001 tgtgcttagg aaattggatg tctttaccgg cttctgtagc gaaaacagat aaagcatcat
    30061 gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt
    30121 cctccaattt tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa aattttcagg
    30181 atgcttttta taatactctt gatggtcttc ttctgctaga taaaatggtt gggctggttc
    30241 gatttttgta acgattggct gttcgaaacg tccactttca gctaactgtt tcttactttt
    30301 ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc accgggcgat aattgtcacc
    30361 gcgatcctcg aattgtccga aagcgtcagt tggatcagtt tgctgccagt aaagttctac
    30421 taattgttca tatgaaatca atgttggatc atagataatc tccactgctt ctgtatgtcc
    30481 agtcgtttgg ctttttactt gttcataagt cggatctggt acatgccctc cagtatatcc
    30541 tgacatcact gtatggatac caggaagcgt gtcgaaaggc tggatcatac accagaagca
    30601 gcctcctgca aatattgctc gttccattat gattccccct ctaatactac tcatccgatg
    30661 attttttatt ttcttttgtc ttgctttcag tactgctggt gctagttttg ttggtatttg
    30721 agttgtttga agcatcagag gtatcttttg gcaaatatag actaaaccga atgtcatcat
    30781 ttactaagtc gattttatct gctttaatat acatcccgtt ttgcattcgg aattgatcca
    30841 acctaagcat gatagtcaga tcatctgtat tgacttcgat ccaatctggt aatttatagt
    30901 ttctcttgat catattcatc acatctttga ttggcaaact caatgttccg atagaaagac
    30961 ttctagcttt tagttggaca tttccgtttt ccatcacata cggatcaaaa tataaataga
    31021 aatcgatagg aaaacctagt acttcgaaag taccattgag tagtgcttca ttttccaaat
    31081 aaaatttata ttcaatatca ctatcttttt gatactcact aaggaaaaag ctgatgattt
    31141 gattgacttt ttctttattt gaattgatcg ttaagacagg atctccttgt ctttccgtaa
    31201 gagctggtat ttctgggagt tctggttccc gattagcaaa aatacggctg cctaaaaata
    31261 cggtcgaacc aagtactacc ccaagaagga taaggaaggc gaatttccag taatttgttt
    31321 tctttgtttt gtttgaagat acgtcagatc ttgttttcat gtttattgat catcctcctt
    31381 ctctaaccat tggttttgtg tctcgatcag ctgatctctc actgcatttg ccatcaattg
    31441 ataacctaaa ttatttggat ggaaatgatc ttcttcatag agcacattat ttttgatgcc
    31501 atttagattt tctgtcgtgc tagttgtttg atctgttcct tcttctgtga cacctacttc
    31561 ctgattcaat ccttgataaa gcaaatcatt gattgggata aagtagcagt tcggcatttt
    31621 ttttgtcacc gtttctgttc ctttgttcca attgttaaca attgtctgca tgtcagtgat
    31681 ctcaggaaag ttcaaataaa atggattgta gatgcctaaa atgtaaatag gtgcggtttt
    31741 attgagggat cgaaattctt ccaatagttc tgtgacattt tcctgatagt ttttcagtgg
    31801 ctttttaaag gaattgaccg ttaacccgaa taaattattt tggataactt tcatcaaatc
    31861 attaccgcca acagtcagtg tgataaaatc agctgttttg atattgtttc gaatttcttc
    31921 ttttgttttt actcgcttta agatctggtc acttcgttca ccatttacac catagttttc
    31981 tgtttcaacc gcggtcaaat tataacggtc ttttagatca tttgcgacaa ggggaacgaa
    32041 tcctccttgc ttggtttgat ctcctactcc ttcagtcagc gaatctccga tagctatgta
    32101 gtgaagtttt tcttttttat ttggatcttc tctcacagta gttactggtt tcattagtgg
    32161 gccagctttc ggtatagaag cagctaaacc tgcatagatg atgatcgcaa ttaataatgg
    32221 gaacaaaacg aggatcaaat gtcgtttcgt taatttcatt ttttcacctc ttgtattcga
    32281 ttggttctaa tgttatcaaa aattttatca aaaaggtagt ctttatgaaa aaagaaaaca
    32341 attattttcc taattttata aagaaaaaga gaataagctg caagcctttc aggctgaggg
    32401 cttatcctct tttcgactaa tctacataat acatgattgc aaacgcgtct ttaccagtat
    32461 gcgtagcgat aaccggattc gtatgcagta cagggatatg catgtccggg aataattctt
    32521 gcaaaccctc tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt
    32581 gtcgcacatt tggcaattct ttcagttcat ttttcaaatt attaaaccat ttatcgaacg
    32641 ttttctttcc tcgcccctta gttacaggga ttaattcaga atgggcgaaa ttcatcacca
    32701 cgcgtatgtt caaaacacta gacagcaaac cagttgttcg gctgattctt ccacctttta
    32761 ccaagttatc tagtgtggat attccgatgt ataattttgt attgtcttgt acttttttga
    32821 cttcttcaag gattttctct actgttttac cttcttttgc taattcagct gctcgaatta
    32881 cctgaaaaga taatccttgg tcagtggtat cactgtcgat aaccgttact ttcgtttttg
    32941 ttagattact ggcttgtctt gctgcttcta ctgttccact caagcctttt gtcatatgga
    33001 tcgaaatcac ttcactgccg tcttctccta gctcatcata catcgagatg aattctccaa
    33061 ttggtggttg gcttgttttg ggcagatttt ttgctttcgc catcatttcc ataaatcttt
    33121 cgccgtctag ttggtcatca tcgggatata ctgttccgtc gatcatgatg gatagcggca
    33181 tgacacgaat gcctaaagca tcgcgtacat ttttttccat cgtacatgaa gaatctgtta
    33241 cgatttttac atttgtcatt attttctctc tctctttctt taacaagatg ccttattcat
    33301 gttaaactac atgtaagaca atttctattt ttagtataac agacaaataa tgatttttca
    33361 ttaattacac ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat
    33421 gaaatattaa atgaagtgtt taatgcagtc acccatggaa ttgggactgg attgagtatc
    33481 gcgggactag tggttctttt agtaaaaggt gcccacttgc attcctccct ccatattgtc
    33541 tcttatgcta tctatggatc gatgatgatt cttttatttt taacgtctac tttgtttcat
    33601 tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt
    33661 cttttgattg caggaagtta tactcctttt tgtttgctga gtatccgagg atggctaggt
    33721 tggactttat ttgtattaat ctggttgttt gcgattcttg gcattatata taaagcgttg
    33781 acgttgcata aacgggagag agtatcaaaa gtatctacga tcatctatat ttttatgggg
    33841 tggctctgtg tcgtggctgc tgttcctctt tatcactctc ttggaatgac tggtgttatt
    33901 ttgatggtag caggcgggct ttcttacacg ataggcgcat tcttttatag cttgaattct
    33961 atccgctata tgcatgttgt ttggcatttg tttgttatgt taggtgcagg ttttatgtac
    34021 ttctctgttt tattctttac ttaataaaaa aaataaagct atggatggga cagctctaaa
    34081 taacagctag tctatccata gcttttttat atcgtcaatt cgcctgtccc gctttttaca
    34141 ggattctcat tacgctgata cgtttcaaat tgatgcggat aacgattttt ttcatccatt
    34201 tctccttgac tagtattgat cagtgaccaa tcagaccagt caacaggtgg aaaataagca
    34261 tctccatcga atacatgatg gatgactgta cggtataata tcgtacaaaa tggcagaaat
    34321 tcttgataaa cagctgaccc tccagcgatg aaaaagatac cgtctgtttc tttttccaga
    34381 gcaagcacgt cttccacact atgcatgaca tgcacgcctt ctgctttata ggaagaatct
    34441 ctcgtcaaaa caatcgtctg acgattcggc aacggacggc tacccatccc ttcgaatgtt
    34501 tttcgtccca tcactagtgt atttgcttct gtcatttgtt tgaagaattt cagatcattt
    34561 ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa
    34621 attgcagcta acattttctc atcctttctg attctaaaca gcgataggag ctttgatcgc
    34681 cggatgtggg tcatatcctt ctaattggat atcttccatc tcaaaatcaa atactgactg
    34741 cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca attgttcttt
    34801 catttgttcg atatgattga tataaagatg agcatctcct aatgtgtgaa caaattcgcc
    34861 aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt
    34921 gaacggaacg cctagaaaca catccccact gcgttgatac aattggcagc ttagccgtcc
    34981 ctcttgaaca taaaattgaa acatcgtatg acaaggaggc aaagccatgc taggaacatc
    35041 ttccggattc catgcagaaa cgatcaatct tctagaatca ggagtctttt tgattgcttc
    35101 gatcacttgt ttgatttgat cgataaagcc accgtctttt gtttcccagt gacgccattg
    35161 atagccgtag atgtgtccaa gatcgccgta tgtttcagca aaggaatcat cggttaggat
    35221 cttctcgcaa aaaagcttgc tctgcttttt atattcttca ttgaattcag gatctgtcaa
    35281 acatctatgg ccaaaatcag tcatatctgg tccattatag tctggacttt tgatgtagcg
    35341 ttcaaatgcc cattcatccc aaatatggtt tttattttgt aatagatagc ggatattcgt
    35401 atctcctttt aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt
    35461 tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt tggtatccga ataagcttaa
    35521 tgtgcctgtg cctgttctgt cttctttacg atgcccttct tccaacaatt ttcttcctaa
    35581 gtcaagatac gcttgttcca tcctttttcc tcctaatctt ctacccattc actcaagtat
    35641 tcccaccgtg ccattttttc ttcaagttgt gcttctgttt cagatacgtc gttttgcaat
    35701 tcttgtaatc gagtgaaatt atctccttga tgattcatct cttcttggag caatgagatt
    35761 ttttcttcaa gttctgcaat ctcatcttca atcgtttccc attctttttg ttccatgtaa
    35821 gataatttct tcttttcagc aggttccttg ctctgtattt ttggtttcgc tttttcagca
    35881 ggctgctggg tctctttttg cttcagtaaa taactggaca tgtttccgta ataaagttct
    35941 tgttttccgt ttccttggaa tactaataac ttatctgcta ccttgtccaa gaaataacgg
    36001 tcatgggaaa cagtgatcac agcacctgga aaacttctaa aataatcttc caaaatcgtc
    36061 aatgttgcta tgtccaaatc attggtcggt tcatccaata ataaaacatt aggttgctga
    36121 atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt gcctatcagt
    36181 gtcccatgca taaatcttgg aaataaaaat cgttcaagta attctgctac tccgatttga
    36241 ctgccatctg ctgtttttgc ttcttcagca gcttcttgta agtaagcaat catccgttta
    36301 tctggtgcca tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata
    36361 gacattgtac cgctatccag gggaattctg ccagctagaa tattaagcaa tgtcgatttt
    36421 cccgcaccat ttttaccagt aatacctagt ctttcccgag attgaatcaa taaatctaaa
    36481 tgttctagca gtgtttggtc atttatacgg taactgccat ctttgatttc cagtactttt
    36541 ttccctaaac gttgtgtcgc taggttcagc tctaaatcat cttcttggtt tacttggtac
    36601 aagttctcct ttagatcttc gaaacggttg atccgtgctt gctgcttggt gccacgtgcc
    36661 tgtacgccag ccctcatcca agctaattcc tgtttataaa gccgcttgcg cttttcttct
    36721 tgttctactt ctacacgatc acgttctgct ttttctaaga cgtaagcttc ataatttcct
    36781 ttatactcgt ataattttcc aaatgaaagt tcgaagattc ggtttgctac tctatctaga
    36841 aaataacgat cgtgggtaac catcaatagt gcgccacggt attgtttcaa atagttttcc
    36901 aaccactcga tcgcttcata atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg
    36961 ggttcatcaa tcaacacttg cgccagacta actcgttttt tctgaccacc tgataaagta
    37021 ctgatttttt tatctaattc actgattcct aatttttgca aaataatctt tgcgttggta
    37081 tctgtagtcc aagcatcttc tttattcatt tgttcttctg ctttagcata acgtttttgc
    37141 acatcagggt cttctccgtt ttcccctagt tcgatcaatg cttcctcata tgctcgaaca
    37201 gtttggatca aggggctgtt tccttgaaag actgcttgga gaacagttaa ttcatcggaa
    37261 aattcagctg cttgagaaag ataaccaata cggtaatcag ttggataaaa cactgtttgc
    37321 cgatcaccgt cgctgctatc gataccggca ataatattta agagacttgt tttacccgtc
    37381 ccgttcgtcc caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga aatctggtca
    37441 aacaaatctt tttcaccata agtttttttg aggtcagtta cttttaattc tttcaacatg
    37501 ttccctcctt atcttggata ttgtatcata aaaatagtga acttcaggaa aatttttttg
    37561 gtatttgttc tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata
    37621 tgttttgttt tttataaaac ttgaaaattt ttaccttttt attttattgg actcgttttt
    37681 tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt gttcatcgta
    37741 taagattcta acaaattgat attcctgcga tagctgttcg cagaaaacgt cgtgattttt
    37801 tcagcaactt cgtattcatc aatgattttt ctttcaagat aataacctct tagcattttt
    37861 ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa tcaaattccc cgagttcatt
    37921 tttattcctt caagtttgcg ttgctggata aaaaattgaa ccatcgtttc atcataaata
    37981 tcagtcaagt tatctaaact ttcaataatc aactgagtat ttttttgata tgtctctctg
    38041 atttcttgga tatcttcatc actaataaat gtctgacgat cagcatatct tctttgccaa
    38101 aacattttcg gatggagaac gattcgcatt aacctacgga caacaatgaa ccaaaagccg
    38161 ataaaagaca aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa acgggagtaa
    38221 aaacgataac cattagaaga aagcttgccc gaacgataac tttcatccaa tccatcccgt
    38281 tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt gcttttcatc atcggttaaa
    38341 tcttccaagt acagatcacg tatccgttct tgataatttt ctatcactgc ttcagtggct
    38401 aaaaattctt ttgtattacg atcaatttcc tttatttctt ttcgcaaaac ttcaacgacc
    38461 tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt ttccgtcagc
    38521 ataggcaaga caatgatccc tatgaccaaa gtcactaaga tgacacccgc cgtcagaaac
    38581 aaaagcaaag accgctggta aaacatcata ttattgattg aaaatggcaa aataaaaatc
    38641 gtagccaaac tgactgttcc ctttacaccg ccaaatgtta agatcaagat ctcgttcaac
    38701 tgttttttga actttttact gccatctttg aatacataaa aaagactgat agaaatgaat
    38761 ctgatcacaa aaagcatgac agagattaat acgataatcg ctaataaaag accgtttgga
    38821 tataaaccgt ctccccatac tggcgagaac acttgcgtca attcgattcc taaaaatata
    38881 aatactaatg cattcagtgt aaaagcaata gttgtccatg tactgtggga caagctcgac
    38941 aattctgcat caaacacaga gattttccta aaaccagaag cttggagaat acctgctgcc
    39001 actgcagcaa tgattccaga tacatcaaat gcctctgcca atacataggc tagaaaagga
    39061 agcaaaagtt cgatcagcag ataccctgtg acatcttgtg ctgatgcttt ttcgatcaaa
    39121 tgaattattt tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc cccaatacta
    39181 gaaactacta gactcatccc cgcgtttact gctgaaaaag atccggtgat cagcgcacca
    39241 agagcaaact ggaaggcagt aacaccagat gcatcattca aaagaccttc tccttccaaa
    39301 atatgcatcg cctttttagg gatattcact cgtccagaaa gagaactgac tgctaccgca
    39361 tctgtcggac ctaacgctgc tccaaaagca aagcaggcag caagtgggac acttggcaac
    39421 aggaatgata acgtcgcacc taccccgaca agggtcaaaa tgactcctcc aaatgccaag
    39481 aacaaaattg tcccgaaatt tttgagaatc gaagaaatat ctgctttttc tccttctcta
    39541 aataacaatg gtgcaataat cataactaag aacaattcag gttcaaaatc gattgattgt
    39601 ccccactctg tcagacctaa taccactcca aggaaaattt ggatcagtgg agctggaatc
    39661 attggcaaaa tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa tacaataact
    39721 aactcagcaa attccatcgt ccatcccctt tattttttat tttgtgtaag catttttaaa
    39781 aattgttctt ttttctttaa aattttggtt tcgactactt caacctgtgt ttggttcccc
    39841 tctaattcac actgtacacg ttttgactcc aaacgaagca gatcatgttt cagacgttcc
    39901 aactgacgat acatcaatat atttctttta cgcaacttac aaagtatttg acggcggtca
    39961 tccatcgaaa gggcgccata gcgatcatgc aacagttttt tttgttgttc aaaactctct
    40021 ctcattttcc agatctccta tacacaaact tccatcatta tagcaaagtt ctttttggat
    40081 gaaaaatatt tcattgtttc atcgtttatt gctagttaga aatttccata aatttttcaa
    40141 cgcctcattc tattccaaat agtatcagat atcaagtaaa acttaagaaa gataaaagtt
    40201 tctgacaaaa ggatgtttac tttcccattc ctgcctttct tgttctttta tcacaacaaa
    40261 aaaatggggc tgcgacaaaa atcttgtcac agtcccttat tctgaataaa cggtgtggaa
    40321 gagacagcct atcagtagta ataagacaca tctctttcac agcctcattt ttgcgtaaaa
    40381 atcgatttat tcgaaaaatt ccccgtatac atcttctaca cgttgatctg gattgatcac
    40441 gatatacaag ttacctgttt tttcgctgat ttttgttgat tgatattgat tgtctaatcc
    40501 ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat ttcggtaaag
    40561 gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc ccatttccag
    40621 tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt
    40681 aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt
    40741 tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc
    40801 atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga ctcctttatg
    40861 atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg gtcacatctt
    40921 atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag ctgagcagat
    40981 acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa cctgtttttt
    41041 atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag cttcactaac
    41101 ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta ctgctaattc
    41161 gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca tctgaggtgt
    41221 ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc ctaagaagta
    41281 caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga caagtgcttg
    41341 aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac gaatctcatt
    41401 ttcttttaga tccagttggc tgatcaataa tgcccatgct tggctctctt ctttcaatgg
    41461 gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca aaccgggaca
    41521 gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt catttcccaa
    41581 gagcatcttg atgaattcca catttatacg ttctacagat attttaccta atagctcgtg
    41641 gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt gactgacaaa
    41701 acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag gattgcctac
    41761 agctcgcaag atctgttctt ctagatcttg cagaccatcg aacaaatcga tgatttctcc
    41821 atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat cttctgccaa
    41881 cgaacgaacg aattcgacat gatctggtct acggtagtcc tgataagttg attccgtccg
    41941 aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc catgttcgat
    42001 cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg cgcttgttgc
    42061 gatatccaca tcatggattg gctggttcag caagatatca cgaacacttc cacctacaaa
    42121 ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct tttggtattc
    42181 cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct tgtcctgaga
    42241 taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt caaggccagg
    42301 accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc tttgattgaa
    42361 tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat tagccatgat
    42421 catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg gttcagaagc
    42481 ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac ctacttcttc
    42541 agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt gctggatata
    42601 ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct ttttccctcc
    42661 aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa
    42721 tttccatgct acatttagga aggaatttct aaatggaaat tctgtgaaaa tacataattg
    42781 aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt tatcattata
    42841 ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca tttagcagaa
    42901 gggggagtct cagggatcac cttgatctta agagctttat ttggtattga tccagcttat
    42961 acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct cggtaaacgt
    43021 tccttggcct atactgttct aggtacactt tctttgtcat tctttttatg gatctggcaa
    43081 agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct acttgcgggt
    43141 ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac aggcggcagt
    43201 gatattatcg ctcggatttt agaacaaaat tacggcatca ctatggggcg ttccctattg
    43261 gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa acgtatgatg
    43321 tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt agatggcgga
    43381 tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc accactactg
    43441 atgaccggtc tgcaaagagg tgtgactttc ctttctggag aaggcgcatt ctctggtaat
    43501 tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc taagaggatc
    43561 gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga agtggaaggg
    43621 caaggcttta cttatttgaa gccacagaca agattactga aaaaactata agaaaaaaga
    43681 caaagatagg ctatgaggaa agattccaca tccagctgac ctttgtcttt tttagtacta
    43741 tactattttt aactaattgt tatgttcact tacccggtta cttttctttt gtgtctgcaa
    43801 aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat ctccatcttc
    43861 tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt ctttttcttg
    43921 aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc aagaagaaaa
    43981 tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt cgaatataca
    44041 ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct ggtctgtgtc
    44101 cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag atagatcact
    44161 gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat tgcttaaaat
    44221 cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc taaaagatac
    44281 atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta gcatttttct
    44341 cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg ttcaagtgtt
    44401 aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct taagaaaaag
    44461 gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg ttcatagtgt
    44521 acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt ccattctgca
    44581 taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc atatctttca
    44641 tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc tcctacttct
    44701 aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa agcattttcc
    44761 gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc ttctaacact
    44821 gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata tagatataac
    44881 gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc ctgctgattt
    44941 tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt cctttcttct
    45001 ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat acttaaggat
    45061 tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct tgccaacaat
    45121 gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc
    45181 gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa ttttgcttca
    45241 ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa gctttgcgga
    45301 tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc gatctcatta
    45361 tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg gtattggttg
    45421 atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag caattcttcg
    45481 cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa aaagatttgt
    45541 gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt tgtattcatt
    45601 tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc tcaataattc
    45661 tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa caagtatttt
    45721 tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg aaatctgttc
    45781 cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt ttagttggaa
    45841 atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta aaaatttttt
    45901 catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt caaacagttt
    45961 accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt cttcgatcat
    46021 cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat acagattttg
    46081 caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt caatctgctt
    46141 tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg gcagattgat
    46201 ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg
    46261 gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg ttgtatgttt
    46321 ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga taaacatgga
    46381 ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg ttaaatgatt
    46441 gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa ataacgggtt
    46501 gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga ttggctgatc
    46561 aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt tcgtcaacgc
    46621 atcttctact tgataatcaa gttccatacg catccctcct cttcttattt ttattttacc
    46681 attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact tgcaaataaa
    46741 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
    46801 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
    46861 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
    46921 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
    46981 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
    47041 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
    47101 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
    47161 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
    47221 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
    47281 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
    47341 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
    47401 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
    47461 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
    47521 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
    47581 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
    47641 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
    47701 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
    47761 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
    47821 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
    47881 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
    47941 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
    48001 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
    48061 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
    48121 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
    48181 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
    48241 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
    48301 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
    48361 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
    48421 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
    48481 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
    48541 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
    48601 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
    48661 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
    48721 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
    48781 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
    48841 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
    48901 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
    48961 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
    49021 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
    49081 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
    49141 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
    49201 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
    49261 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
    49321 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
    49381 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
    49441 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
    49501 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
    49561 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
    49621 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
    49681 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
    49741 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
    49801 aactttttta gcatctaaac ggcgttttga aagtagatag ctgccgtttt ctttatcttt
    49861 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
    49921 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
    49981 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
    50041 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
    50101 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
    50161 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
    50221 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
    50281 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
    50341 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
    50401 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
    50461 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
    50521 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
    50581 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
    50641 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
    50701 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
    50761 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
    50821 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
    50881 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
    50941 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
    51001 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
    51061 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
    51121 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
    51181 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
    51241 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
    51301 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
    51361 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
    51421 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
    51481 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
    51541 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
    51601 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
    51661 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
    51721 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
    51781 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
    51841 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
    51901 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
    51961 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
    52021 ctgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
    52081 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
    52141 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
    52201 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
    52261 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
    52321 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
    52381 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
    52441 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
    52501 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
    52561 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
    52621 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
    52681 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
    52741 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
    52801 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
    52861 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
    52921 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
    52981 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
    53041 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
    53101 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
    53161 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
    53221 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
    53281 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
    53341 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
    53401 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
    53461 aaaaggtgga tacatctgct ttgttgcaga taaaccgctg actcatcatt gttttttgcc
    53521 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
    53581 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
    53641 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
    53701 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
    53761 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
    53821 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
    53881 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
    53941 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
    54001 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
    54061 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
    54121 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
    54181 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
    54241 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
    54301 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
    54361 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
    54421 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
    54481 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
    54541 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
    54601 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
    54661 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
    54721 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
    54781 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
    54841 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
    54901 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
    54961 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
    55021 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
    55081 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
    55141 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
    55201 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
    55261 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
    55321 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
    55381 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
    55441 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
    55501 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
    55561 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
    55621 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
    55681 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
    55741 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
    55801 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
    55861 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
    55921 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
    55981 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
    56041 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
    56101 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
    56161 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
    56221 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
    56281 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
    56341 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
    56401 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
    56461 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
    56521 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
    56581 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
    56641 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
    56701 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    56761 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    56821 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    56881 agaaaaaaca ctcactgaat ggagctattg acttatccac gtgcttatcg atagccgttt
    56941 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    57001 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    57061 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    57121 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    57181 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    57241 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    57301 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    57361 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    57421 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    57481 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    57541 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    57601 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    57661 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    57721 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    57781 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    57841 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    57901 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    57961 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    58021 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    58081 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    58141 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    58201 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    58261 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    58321 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    58381 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    58441 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    58501 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    58561 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    58621 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    58681 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    58741 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    58801 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    58861 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    58921 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    58981 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    59041 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    59101 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    59161 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    59221 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    59281 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    59341 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    59401 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    59461 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    59521 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    59581 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    59641 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    59701 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    59761 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    59821 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    59881 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    59941 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    60001 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    60061 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    60121 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    60181 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    60241 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    60301 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    60361 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    60421 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    60481 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    60541 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    60601 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    60661 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    60721 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    60781 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    60841 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    60901 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    60961 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    61021 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    61081 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    61141 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    61201 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    61261 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    61321 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    61381 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    61441 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    61501 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    61561 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    61621 gtctgtgacg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    61681 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    61741 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    61801 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    61861 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    61921 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    61981 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    62041 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    62101 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    62161 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    62221 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    62281 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    62341 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    62401 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    62461 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    62521 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    62581 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    62641 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    62701 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    62761 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    62821 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    62881 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    62941 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    63001 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    63061 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    63121 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    63181 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    63241 tagacactca gacggactgg tttctttttt ctttttcata gcattcctga cagattccat
    63301 ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact acttgctcca
    63361 cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag catagtaaat
    63421 gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat ccatcattaa
    63481 aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga taaacagtag
    63541 caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct gcagataaat
    63601 gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa
    63661 accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca
    63721 tttactcaac cccaacaatt tataataatt ac
//
LOCUS       DAJSVF010000006        60567 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSVF010000006 DAJSVF010000000
VERSION     DAJSVF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.006"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(71..919)
                     /locus_tag="ON550_000352"
     CDS             complement(71..919)
                     /locus_tag="ON550_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3953466.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1143..3083)
                     /gene="thrS"
                     /locus_tag="ON550_000353"
     CDS             complement(1143..3083)
                     /gene="thrS"
                     /locus_tag="ON550_000353"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR3953467.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3420..3599)
                     /locus_tag="ON550_000354"
     CDS             complement(3420..3599)
                     /locus_tag="ON550_000354"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR3953468.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3646..5469)
                     /gene="cadA"
                     /locus_tag="ON550_000355"
     CDS             complement(3646..5469)
                     /gene="cadA"
                     /locus_tag="ON550_000355"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3953469.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5747..8026
                     /locus_tag="ON550_000356"
     CDS             5747..8026
                     /locus_tag="ON550_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3953470.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8119..8766)
                     /locus_tag="ON550_000357"
     CDS             complement(8119..8766)
                     /locus_tag="ON550_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953471.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8945..9664)
                     /locus_tag="ON550_000358"
     CDS             complement(8945..9664)
                     /locus_tag="ON550_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953472.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9795..10946)
                     /locus_tag="ON550_000359"
     CDS             complement(9795..10946)
                     /locus_tag="ON550_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR3953473.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11163..11903)
                     /locus_tag="ON550_000360"
     CDS             complement(11163..11903)
                     /locus_tag="ON550_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3953474.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11907..12230)
                     /gene="rsfS"
                     /locus_tag="ON550_000361"
     CDS             complement(11907..12230)
                     /gene="rsfS"
                     /locus_tag="ON550_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR3953475.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12287..12880)
                     /gene="yqeK"
                     /locus_tag="ON550_000362"
     CDS             complement(12287..12880)
                     /gene="yqeK"
                     /locus_tag="ON550_000362"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR3953476.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12864..13514)
                     /locus_tag="ON550_000363"
     CDS             complement(12864..13514)
                     /locus_tag="ON550_000363"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR3953477.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13524..13835)
                     /gene="yhbY"
                     /locus_tag="ON550_000364"
     CDS             complement(13524..13835)
                     /gene="yhbY"
                     /locus_tag="ON550_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR3953478.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13851..14960)
                     /gene="yqeH"
                     /locus_tag="ON550_000365"
     CDS             complement(13851..14960)
                     /gene="yqeH"
                     /locus_tag="ON550_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR3953479.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14957..15484)
                     /locus_tag="ON550_000366"
     CDS             complement(14957..15484)
                     /locus_tag="ON550_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR3953480.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15664..16449)
                     /locus_tag="ON550_000367"
     CDS             complement(15664..16449)
                     /locus_tag="ON550_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR3953481.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16442..17311)
                     /locus_tag="ON550_000368"
     CDS             complement(16442..17311)
                     /locus_tag="ON550_000368"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR3953482.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17312..18691)
                     /locus_tag="ON550_000369"
     CDS             complement(17312..18691)
                     /locus_tag="ON550_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR3953483.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18699..19118)
                     /gene="fabZ"
                     /locus_tag="ON550_000370"
     CDS             complement(18699..19118)
                     /gene="fabZ"
                     /locus_tag="ON550_000370"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3953484.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19143..19619)
                     /gene="accB"
                     /locus_tag="ON550_000371"
     CDS             complement(19143..19619)
                     /gene="accB"
                     /locus_tag="ON550_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR3953485.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19622..20857)
                     /gene="fabF"
                     /locus_tag="ON550_000372"
     CDS             complement(19622..20857)
                     /gene="fabF"
                     /locus_tag="ON550_000372"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR3953486.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20876..21613)
                     /gene="fabG"
                     /locus_tag="ON550_000373"
     CDS             complement(20876..21613)
                     /gene="fabG"
                     /locus_tag="ON550_000373"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR3953487.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21625..22542)
                     /gene="fabD"
                     /locus_tag="ON550_000374"
     CDS             complement(21625..22542)
                     /gene="fabD"
                     /locus_tag="ON550_000374"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3953488.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22649..22873)
                     /locus_tag="ON550_000375"
     CDS             complement(22649..22873)
                     /locus_tag="ON550_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3953489.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22926..23888)
                     /locus_tag="ON550_000376"
     CDS             complement(22926..23888)
                     /locus_tag="ON550_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR3953490.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23898..24338)
                     /locus_tag="ON550_000377"
     CDS             complement(23898..24338)
                     /locus_tag="ON550_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3953491.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24644..25609
                     /locus_tag="ON550_000378"
     CDS             24644..25609
                     /locus_tag="ON550_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3953492.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25666..25896)
                     /locus_tag="ON550_000379"
     CDS             complement(25666..25896)
                     /locus_tag="ON550_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR3953493.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25982..26869)
                     /locus_tag="ON550_000380"
     CDS             complement(25982..26869)
                     /locus_tag="ON550_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3953494.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26958..28181)
                     /locus_tag="ON550_000381"
     CDS             complement(26958..28181)
                     /locus_tag="ON550_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3953495.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28198..29244)
                     /locus_tag="ON550_000382"
     CDS             complement(28198..29244)
                     /locus_tag="ON550_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3953496.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29408..31135)
                     /gene="ptsP"
                     /locus_tag="ON550_000383"
     CDS             complement(29408..31135)
                     /gene="ptsP"
                     /locus_tag="ON550_000383"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3953497.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31135..31401)
                     /locus_tag="ON550_000384"
     CDS             complement(31135..31401)
                     /locus_tag="ON550_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR3953498.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31880..34114
                     /locus_tag="ON550_000385"
     CDS             31880..34114
                     /locus_tag="ON550_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3953499.1"
                     /translation="MLCQNCGKNEATIHLYATINGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34262..34582
                     /locus_tag="ON550_000386"
     CDS             34262..34582
                     /locus_tag="ON550_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3953500.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34787..35011)
                     /locus_tag="ON550_000387"
     CDS             complement(34787..35011)
                     /locus_tag="ON550_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCR3953501.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35209..36789)
                     /locus_tag="ON550_000388"
     CDS             complement(35209..36789)
                     /locus_tag="ON550_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR3953502.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37190..38566)
                     /locus_tag="ON550_000389"
     CDS             complement(37190..38566)
                     /locus_tag="ON550_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3953503.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38694..39812)
                     /locus_tag="ON550_000390"
     CDS             complement(38694..39812)
                     /locus_tag="ON550_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3953504.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39954..40388
                     /locus_tag="ON550_000391"
     CDS             39954..40388
                     /locus_tag="ON550_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953505.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40466..40999)
                     /locus_tag="ON550_000392"
     CDS             complement(40466..40999)
                     /locus_tag="ON550_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR3953506.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41093..42271)
                     /gene="mutY"
                     /locus_tag="ON550_000393"
     CDS             complement(41093..42271)
                     /gene="mutY"
                     /locus_tag="ON550_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR3953507.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42264..43061)
                     /gene="recX"
                     /locus_tag="ON550_000394"
     CDS             complement(42264..43061)
                     /gene="recX"
                     /locus_tag="ON550_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR3953508.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43168..44550
                     /gene="rlmD"
                     /locus_tag="ON550_000395"
     CDS             43168..44550
                     /gene="rlmD"
                     /locus_tag="ON550_000395"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3953509.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44724..45302)
                     /locus_tag="ON550_000396"
     CDS             complement(44724..45302)
                     /locus_tag="ON550_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR3953510.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45624..46094
                     /locus_tag="ON550_000397"
     CDS             45624..46094
                     /locus_tag="ON550_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR3953511.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46202..47428)
                     /locus_tag="ON550_000398"
     CDS             complement(46202..47428)
                     /locus_tag="ON550_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3953512.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48182..48382)
                     /locus_tag="ON550_000399"
     CDS             complement(48182..48382)
                     /locus_tag="ON550_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3953513.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48516..49124)
                     /locus_tag="ON550_000400"
     CDS             complement(48516..49124)
                     /locus_tag="ON550_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR3953514.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49209..49679)
                     /locus_tag="ON550_000401"
     CDS             complement(49209..49679)
                     /locus_tag="ON550_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR3953515.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49727..50641)
                     /locus_tag="ON550_000402"
     CDS             complement(49727..50641)
                     /locus_tag="ON550_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3953516.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50787..51590
                     /locus_tag="ON550_000403"
     CDS             50787..51590
                     /locus_tag="ON550_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR3953517.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51768..52715
                     /locus_tag="ON550_000404"
     CDS             51768..52715
                     /locus_tag="ON550_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR3953518.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52783..53076)
                     /locus_tag="ON550_000405"
     CDS             complement(52783..53076)
                     /locus_tag="ON550_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR3953519.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53104..53769)
                     /locus_tag="ON550_000406"
     CDS             complement(53104..53769)
                     /locus_tag="ON550_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR3953520.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53909..54541)
                     /locus_tag="ON550_000407"
     CDS             complement(53909..54541)
                     /locus_tag="ON550_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3953521.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54548..55615)
                     /locus_tag="ON550_000408"
     CDS             complement(54548..55615)
                     /locus_tag="ON550_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3953522.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55612..56343)
                     /locus_tag="ON550_000409"
     CDS             complement(55612..56343)
                     /locus_tag="ON550_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR3953523.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56512..56991)
                     /gene="greA"
                     /locus_tag="ON550_000410"
     CDS             complement(56512..56991)
                     /gene="greA"
                     /locus_tag="ON550_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR3953524.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57127..58302)
                     /gene="mltG"
                     /locus_tag="ON550_000411"
     CDS             complement(57127..58302)
                     /gene="mltG"
                     /locus_tag="ON550_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR3953525.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58506..59675
                     /locus_tag="ON550_000412"
     CDS             58506..59675
                     /locus_tag="ON550_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3953526.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(59942..>60567)
                     /locus_tag="ON550_000413"
     CDS             complement(59942..>60567)
                     /locus_tag="ON550_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953527.1"
                     /translation="APWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAI
                     SILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTED
                     GQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTV
                     KSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        17618 a        12672 c        10194 g        20083 t
ORIGIN      
        1 atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta tataaaaaat
       61 agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt gtttttttca
      121 ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata tcgatcgtag
      181 caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct tttggtctag
      241 atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat tgcgtcaacg
      301 caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca tctgcacttc
      361 tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca agttgcggga
      421 gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac catttttgca
      481 gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca gtctctgccg
      541 attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct actgtttctt
      601 ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag gactcatttg
      661 cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa atcgtgattt
      721 tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact gtcggcgcac
      781 tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc tcaatattac
      841 cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc atactttcaa
      901 tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat gtaagtatac
      961 catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac aatccgccca
     1021 acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt ctttgaggaa
     1081 tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag gaaatttacc
     1141 tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat aaagatattc
     1201 aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt tgcatcttcc
     1261 aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga ggcacgtatc
     1321 ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt catttgcaaa
     1381 cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt
     1441 gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt taaataagcg
     1501 acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg atgcgtattt
     1561 tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa gtctaattgt
     1621 atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc tagttttggt
     1681 ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc atccatagcg
     1741 gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata tttatctgta
     1801 ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa gtcagcatat
     1861 actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc aggacgaaca
     1921 aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga caaagcacct
     1981 gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa ttcacgataa
     2041 gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg gcgaagaaca
     2101 agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata atgatcccag
     2161 tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac atgttggtaa
     2221 cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat tgttgcccct
     2281 tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa caagtcaagt
     2341 tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg gatatattct
     2401 ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat ttgattgttt
     2461 gagtttccac gccaataagc acctgccact gataataatt taaatacttg tatacggcca
     2521 gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg ataagcagta
     2581 atcacttctt cttctggcaa ttcactaatc agttctactt tgtacggatc acttgcaaaa
     2641 atttctaatg cttcctgttt tgaaagaaca cggcgttcga tcggcagatt ttctttgacg
     2701 attttcatca tttctgcttc gattgccggc aaatcttctg cagttacttg gttttgtcca
     2761 ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa gtggatattt
     2821 ggatagaggc gacgcatggc ttgggccatc aaatgagctg ctgaatgacg gactaagcca
     2881 agtgcatctt catggtctgg tgtgatgatc tcaatacttg catcttcctc gattggacga
     2941 gtcaaatcaa cgagttttcc gttcacttta ccagctaaag ctttttttgc taagcttttt
     3001 gaaatgcttt cagcaattgc caatgttgtt gttccagact caaattcttt gactgcacca
     3061 tctggaaaag taatttttat cataatagtt tcctccttga aatgttttga ttgatcagat
     3121 catcaagctg aaaaacaaaa aaatcctagc atctcttgaa aagagatact aggacgaaaa
     3181 gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg
     3241 accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa
     3301 gtttcagcct agcttttcct ctcttatcga agactctcaa attcagttgg ctttctcatc
     3361 aatcaattat gtgtttattt aaccactaat aaatagaaaa atcaagtggg aaactaaact
     3421 tattttttcc attccccatt tacaccgtat gttccttttt tcatctcatt gatgatgaca
     3481 tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat atctttcatc
     3541 atattttcta attgttccgg actgcgacct tctactaatt cgacatgtac gaatggcata
     3601 atgctctctc ctctttctga tcgttctttt ttcaactcga ttctatcata ctatgtttgt
     3661 cttgggtact gaatcaggaa aaatggctgg atcttttttt gctctcaagg cactcaaaat
     3721 agagaccgta tccacgactt cttgcaaaac agctccgaat aatgcgggga ttagtcccgt
     3781 acttgcaatt aacatcaaaa aaatacaaat aaagatgcca atcagtacag actgtttcgc
     3841 tattctcatc gtatctcttg aaatcactac tgcttttgct acacgcaaga gatcatcttt
     3901 caagatgacc gcatctgcac tttcactagc agctgtagaa ccatgagccc ccattgcaat
     3961 accgatatct gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac
     4021 aggtctttga tctttaggga tactttctaa aatggtcagt ttatcttgag gcaagctacg
     4081 ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg cagatccatc
     4141 cccggtgatc atcatcaaat ttttcacatc aagttttctt agctccttga ttgtctttgc
     4201 tgcttctggg cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt
     4261 tacatagatt gctgtattgg acgtttctat ttcttcagct gaaacaaaag aggcacgtcc
     4321 aatctttact tcatgattat tgactactgc ttggattcct tgaccggtca cttctttcag
     4381 ttgactaaca ggatacagtt ggctgttttt cacgttgttc actaacgaac gagctaagat
     4441 atggctagat tcctgttctg cactagctgc atacagaagt agctcttctt tttttattcc
     4501 attgactggc tgaatcgcct ctacttccaa tatccctttg gtaagcgttc ctgttttatc
     4561 aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat
     4621 cccgttttta cttgaacggc tcattcctgc aactaaagca atgggcgcag ctagaatcag
     4681 tggacaaggt gatgccacta ccagtacctc agcaaatcgt aaagggtctt ttgagaacca
     4741 ccaagaaata ccaccgatca aataagcaat caacgtgaaa gggactgcat aacgatcagc
     4801 taaacggaca aatcgggcag gcttttcttt tgattcttcg actagtttaa taatcatttg
     4861 atactggctg tctgcggcac gtttttcagc gatcatttgg atcgatccgt ctccatttaa
     4921 agaccctgac ataatctgat ctccaggttt cttggtttcc gggcgggact cacctgtcaa
     4981 agaagattca tcgaccgaag attctccttt ggtcactcga ccatctgcgg gtacaagttc
     5041 gccaggcttg actagtaact ggtcatcaat atttatttca tcgattacta tatcctgcaa
     5101 ttttccttct ttgaatcgat gggcaattct tggggtgtta tctaacaaag actgcagttc
     5161 tttacccgct cgtttattcg cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa
     5221 tatcatcaaa ctagcccagt attctttgac ggctaatgtt gcaaggattg cggtaatcgc
     5281 taatacatct acgccatatc ttcctgaacg taacgtcttg atcatttcca aaaacatcaa
     5341 gaaggcgaga atccctccag taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa
     5401 aacaaattca cttaataaag ctatcccacc aataagcaaa gtaagtaaaa attttttgaa
     5461 atgtgtcatt gttttttctc ctcttttatg tgacttagag aggttagtaa taaatttcca
     5521 gctgcctctt tcatcttaag catagtcctt gactagcact ttttaaaaca ataggcctta
     5581 attgaaaaca actatcacta tcagcgtttc caaaattcat cagttctaca aacaaaaata
     5641 ttcagcaagg attacttttt cgattcatca gttaaaaagt accttttttc gaaaaactat
     5701 gctatacttt taattcgtta tttacggcgg aaggagcaaa catgcgatga atgagaaaca
     5761 actagaacaa acgcacgtgg atgaaacgat cgaactaatc cagatcgaac aaaatatgct
     5821 ggatacaaaa caacaagaat tgactcagga aatgaatcat tcgacaaaag acacagctaa
     5881 tcacaagatt cgagctggtt ccaatgaagc tttttatgaa tctgctgttg aatatcgcca
     5941 acatgagcag gaattgcttt tgaaatacca tactcttgaa gcacagcaaa aacgcctgca
     6001 aacgttgtct gtcatgaaag atagtcctta ttttgcccgt atcgacttta aagaagaccg
     6061 tgaaacagaa acattatatc taggtatcgc ttctttaaga gatctggcag aagaaacaat
     6121 cgttattgac tggcgcgcac caattgccaa tctttattat gaaggtgaga ttggtcccgc
     6181 attttatgaa actgaaacag atagaataga tgtcgatctg cagttgaaac gacaatttaa
     6241 aatccaagaa ggacgtattt tatctatggt agatacgtcg gaagtaatca atgatgactt
     6301 tttgctagag atattggatg aatcatcaag caaccatatg aaaaacatcg tctctacgat
     6361 acaaaaagca caaaatgcaa tcattcggga cacaacaagt aaagtaatgc tgattgaagg
     6421 aatcgccgga agtggaaaga catccgcttt gcttcaacgt attgcttttc tgttgtatca
     6481 taatcgcaag tggctcgagg ctgaaaacgt tcttctattt tcacccaacc atttgttttc
     6541 tgattacatc tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta cacagacatt
     6601 caaaaattat ttggtgcagc tgctgccaga gttttcttta ctagatgaac agcaacaaga
     6661 aagtggtttt ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg gattgacctt
     6721 agtcgatcag attgatcgct atatccaagg catcacaaac tacggtccct tgttccgtga
     6781 tatgaaaata aatggccgta cgatcctatc aaaggaatcg attcgccaat ggtataaaga
     6841 aacaaatgaa caattacctt tacatcatag actttctcta ttacaaacga agctcctgaa
     6901 aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag atctggcgga
     6961 agaacagctc caagaactat acgcaacaga tccaaatctt gaatatacag aacaaaagga
     7021 gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc gtcgaatcca
     7081 tcgagggatc atgaattatc aatttgtcaa tataacgaaa caatatctcc actttctaca
     7141 aactgtgcca ctttctcttc taacacgaca taaaatcagc gagaccaggt ggcaggaaca
     7201 tttgacaact gttcgtacac acttgcgtca aagagaattg cgtcaagagg atgctgtact
     7261 attcttttta ctgatgcgcc gaacatatcc agtgacggtt acacacaaag ctcgctatat
     7321 ctttattgat gaaatgcagg atttcgcacc atcacaagtg gcattgctgc aagccttgtt
     7381 cccaaaagca aatttgactt tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac
     7441 gatcgttgga tcattggaag accttttcgt ggaccgtgaa gtcactcgtt tccaattaac
     7501 gacaagctat cgctccacaa aagaaatcac tgattttgct aatcatttta ttgtgaaaga
     7561 acatcaagta gaaacaacag caagaaaagg agaccttcct ttgctcatcc aaggaacaac
     7621 agatgatatc aaattgaatt ggttagcaga taccattcga acgactcagg gaaaagcccg
     7681 gtattggcgt acagcaatca tagctaaaac acaagaagaa tgcgaagctc tttatgctca
     7741 actgccagac gagttgaaac aacaagtcca gctgatttta aatgagagtg actttatgaa
     7801 gcgttccatt attttacttc cagccttttt agcaaaaggt ctggagtttg accgagtttt
     7861 cttgtgggat gtagatgcag aacatttttc ttctcagcaa gacaaattga tcctttacac
     7921 catgtgtaca cgtgcgatgc atgaattgac cctttttacc tccaataatg aaagtccttt
     7981 gttaaacaat atggatattt catcttatga gaaaaaagta ctgtaagtaa aaagacaact
     8041 gctatcatta atgttagcag ttgtcttttt atttacaata ataattgcag aaaaagtaat
     8101 tcaatcattt aaggtcattt attctatata taaatccaaa tctgtttcag gatattttaa
     8161 tttgacaagc gaaataatca ttttcagttc ttccatgttt tctggatatt ctgggtcaaa
     8221 taccttgcta tgacggccaa tcccgccatt gtcgatcaat cggtaaattc gattccattc
     8281 aatgaattgg tcaattgatt ggaaattgct gattggatag ccgccacaat ccaaaatctc
     8341 tgatacacga aatgctaggt atgcactatc gatcacttta ttttttttcg tgtctatctt
     8401 ctcatttctt tcgataagaa ttttttcagg acgatgtttc aagctccact ctaatagtag
     8461 aaagaaagcc tccatccgat actcttcttc aaataaccga aaggctgaat cagaacgacg
     8521 aaaaagcagc ggaatcagat aattttgtct ataaatcagc attggtgcgc ggtccaccat
     8581 acactctaaa taacggttca gataccctgt accgacatag tttcctaatc gatagccttc
     8641 aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa tcagttacgt tcacagctaa
     8701 ctcttctact ttgcggtaac cgcttaagat acgttcaaaa ggttctaaag tgatttttgc
     8761 ttccatattc ttccccgtcc atcttccaca tacttcatat catttcacat gatacttatg
     8821 ctctcatcat accacaggtt gaattagaaa agaaaataaa tcacacaaaa aagcaagaca
     8881 atctgttagt gagattgtct tgcttttcga aaaagactga atacttttcg ttaaaactgg
     8941 ttgcttagtc ttctaatgcc acattatgga acacatgctg tacatcttct agatcttcca
     9001 gtgcatcaat cattttttca aatgtttctt gatcttctgg agatagtgtt acctcgtttt
     9061 gagggatcat ttggatctca gcgattgaaa aatcagtgat tcctttttct tttaatgctt
     9121 cttgtatact atggaaatcc gctacttcac cgtaaacgat gatttgtcct tcttcttcca
     9181 ttacatcgcg aacatcgatg tctttttcca ttaggtattc aaggatctcg tcagcgtctt
     9241 ctcctgcaaa agcaaagatt gctgtattgt caaacatgta agaaaccgct ccggaaacac
     9301 ccatgttccc gccatttttc ccaaatgctg cccgtacgtc tgctgctgtt cgattcacat
     9361 tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg tccaaatcct tcatagcgca
     9421 attcagagta tgtttcatct cctgcacctt ttgctttttc gattgctcgg tcaataatat
     9481 gttttggtac attataagtt ttagcgcgat caatgacgaa acgtagtttt tggtttgcat
     9541 gcggatcagg atcacctgat tttgctgccg cgtagatttc aatcccaaat tttgcataaa
     9601 tccggctatt attagcatct ttagcagttt tctttgctac gatatttgcc catttacgtc
     9661 ccatattttc acttcgcttt ctgatttgat agtttcataa cgcaaaaacg tcctgttcca
     9721 ttatacttgt gaatcacgat tctgtgaagc tttttttctt tcttccttca ttcttctcat
     9781 ttttagtcaa ttatctatac tcggattggg atttgcccga aattctgttc cggaatattg
     9841 cctcccaatt gatagatttg atcacttcta actaacagtt ccgcttcttg tgcctctttt
     9901 tttccgattc gggaataaat agaaatctct tttttttctt ttaagaatct tcttccagca
     9961 tcggtaaatc caagtagtcg aagataattt ttattttgtt ggacaggaat ctcttgcttt
    10021 ttgatattca gcaagacata acaagataat cgttggatcc gtgtccatgt atatcttttt
    10081 gtttttacct ttgacacaaa atcctcaaaa ctatcagcag ttgcagctgc tgttttcatg
    10141 cgcgattcta ttccttcggt catctgatag atatctgtca gttccagcaa agtagaagat
    10201 aacaaccgat aacgcaaata cggccaataa gattcccaag tctgcagcga acaagttgaa
    10261 agatcttctt ctgtctgagg cggcacaagt ccagctattc tttttccttc ttctaacgca
    10321 tggcgaatgg ctgttgcact ggctatcctc tgatctgtga tgattttttc atgatagcct
    10381 gcacttttac gtcggatcgg atgtaattcc atagggtgga catacttggc attttccttt
    10441 gcataactta atcccaagat atgatttggc gaagaaaaat caagcgtaat atcgggatac
    10501 agactgcgaa aaacctcagt cattttttgc ggataactat acgtttgatc agacagttct
    10561 tgaaatgcct cttctatcct atcttgattc tcttggacaa agcgtccgaa actgctgtaa
    10621 tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt cacagtccag tgtttgcaag
    10681 atttttactg cacctttagc aaaatagtca gctgactgaa ccgcccattc gacgggaagc
    10741 tccactacta aatccacacc attttgtaaa gcttcattcg cgcgatgcca tttatcgatg
    10801 atagccggtt cgcctctttg caaaaaattc ccgctcatta cagcaatgac gacctctgct
    10861 cctgttattt ttcgtgccat ttccacatga tagcgatgtc cattgtgaaa aggattgtac
    10921 tctacgataa tcccgcagct tttcatgcgc atcctccttc atagttatcg ataaaaaaga
    10981 aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata cccgaactat ataaactatt
    11041 ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc aagagcaatg cctatatcgt
    11101 tcggatatat aatttatgtt gaagatttat tcttttcttg ttaactcgag caggcaaaac
    11161 cgctagtctt tgtatgctac aaagaaccag cgctttgatt tctctgatgc tgcttcgtct
    11221 gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt tttccagcat tcttaaataa
    11281 ctttccaatg gataggtacg ctcttgatgt agttcatctt cacgaataaa tcgaccagac
    11341 ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga tgctgtgttt ctcgcttcct
    11401 ggataactat cccacaaaaa agcaaaatta tccccttgat aatgatagct atattctgga
    11461 aaaacctcat ctacttgata ggtggagtgt acgtcaaata aaaatcggcc attttcttct
    11521 aacgcttgat agacttcatc aaacacttgc tgtacctctt gtctattttt catatagcac
    11581 agcgaatcag aaaaacaggt aattgcctga tattgtccga tttctgataa atcgagcata
    11641 ttgccttcca caaattggat cgatgcgtct tcttcatatg ctcgctggct ggcaatcatc
    11701 aacatctctt ctgaaaggtc taaccctgtc actttatatc catcttttgc aaaggcaacc
    11761 gctaatgctc cagtaccgca tgccagttcc aaaatatttt ttgtgtcttc ttgtaaatga
    11821 cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt ccatcacttc atcgtaaatg
    11881 aaagcaaatg tttcataagc catatgttaa tccaaccatt cactaagatc gactagcgga
    11941 gcatctgacc atagtttttc cagattatag aaatttcgtt ctggcgcatg gaaaacatgt
    12001 acgatcacat ctccaagatc gatcaatacc cattttccgc cttcttttcc ttcaatacgt
    12061 tttacttcat atttgttttc ttcttcttta tcaatgattt cttctgtaat tgcgttgatt
    12121 tgacgttcgc tatttgctga acagatcatg aaataatctg ccaacaagga aacatttcga
    12181 acgtctaaag cgataatgtc ttctgcccgt tttgaatctg ctgcttcaat cgcaattttt
    12241 aattgttctt tactatctat gatgatttcc tcctaatatg agcagcttat ttttttgcta
    12301 cccagtaatt atatgtttcg attgtttttg gataaatctg ttgttcttgc gcaagcaaat
    12361 gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc tagatcagcc catgcaatgg
    12421 ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc aatatagtct gctacataaa
    12481 tgattttgtc aaggagactc atcttagcag ccccagtcgt atgcacccgg attgcatgaa
    12541 gaatctctgc atcggtgatt cctaattccc gctctacaaa cgaagcgcca acaagaccat
    12601 gccagattgc attattataa ttcaataact ccggatcaaa accgtctctt acgattacca
    12661 ttttgaactc ctcatctggt cgttcttttg cataatcatg tgtcaatgca gcaatactag
    12721 ctttttcagg agaagcaccg tattttttgg caagagccac ggctgtttct tctactccaa
    12781 gcacatgctt gaagcgttgt tcgctcattc tcatctgtat tttttgcatc aggttttctc
    12841 tagaataagc agtatattca ttactatagt ccatattcat acaacccttc attttgaata
    12901 taatcaatta ctttatctgg aacaaggtag cggatagaac agccttcttt gatcttttga
    12961 cgtattttcg tggaactgat gtcgatctca ggtacatcta cccaaattac aggatatgga
    13021 gtatccgttg tgtatcctgg tcgacgtata cccacaaagt tgaccataga agtcagctca
    13081 tcgattttat accattttgg caaatattct accatatccc caccgataat aaagtaataa
    13141 tctgtatccg gattgttttg cgtcaattcc ttcatagtat cataggtgta acttttccca
    13201 ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat tgtcttccac cgctaattcc
    13261 aacatgttga gtcgatgttt tgcatcaatc gtctgtttct catcaacatg aggtggctga
    13321 taagaaggca tcaaaaatac gcggtcaaga cctaaattcc ggccagcttg ttcagccatc
    13381 actaaatgtg caaggtgaac gggattgaat gttccgccca agatccctac ttgctttttc
    13441 ttctctaaga attgaggcat ttcttcagga aataattgtg aacgaacaaa tgtcttagct
    13501 tgcgtattca tcatggtcac tccttatatt gcttttactt cttttgatag cctttgatac
    13561 ttttcttttg tggaaggttt gtataataca ataacacgtc caatgatttg gacgatttcg
    13621 caatctattt ctttttctaa tacttgagct gcttcttctg caatctcatc tgtattttga
    13681 agtaacgata ctttgatcag ttctcttttt tctaaagctt cttcaatttg aacgatcatt
    13741 gctgaattga tcccaccttt tccgatttga aaaatcggct gaagatgatg cgctttgcta
    13801 cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt tccttcttcc ttaaatcaat
    13861 gctttgcgtt tcaatacatc tacacccttt ggcgcccagc ccgcaattac tactggcgct
    13921 gttacagtga tccagcctag accggcaaaa acgatgtctg ttttttcttt cacagaaaac
    13981 tcaaaacgaa caagctccgg aaatgcctcg acttcatcag gacgaggagg ctgtaataat
    14041 ccacctacat gtttttgata aaattcgtct gctttttcca gtttcgttcg atgaatattt
    14101 acatcatttg acacataagc aataaatgaa gcacgattgc cgctgataaa atcaaatctt
    14161 gcaagtccgc ccaagaaaag tgtttgccct tcattcagct gataaacttt aggtttgatt
    14221 tcttttaacg gtgcaatgat ccgcaaatct tttttaccta agtagtgcgc catttgatgg
    14281 cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt catctaaagg aatctcgatt
    14341 ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta catcttgtac tcccgctgta
    14401 tttttgatga tttgattgat aagcgtcgat tttccgacat ttgttactcc gacgatatag
    14461 acatcccgtc catcgcggta ttcttcgatt ttttctaata gttccgtcat ctcatgagat
    14521 ttcttagcac tagttaataa tacatctact ggacgaattc ctgcttcgta agcacgttcc
    14581 ttcatccatt gtgttaattt agtttttttc aatgatttag gtaatatatc cactttgttc
    14641 ccaactaaaa gtactggatt gtcaccgaca aaacggtgta gtcctgggat tagtgaacca
    14701 ttgaagtcaa aaatatccac cacattgacg atcaatgcat ctttttgccc taattcattc
    14761 agtaagcgca aaaagtcgtc atccgtcaat gagacatctt ggatctcatt ataatggcgt
    14821 aatctgaaac aacgctgaca atagacttcc ccggaagcta aacctttttc caaagccgtt
    14881 tgcggcgtgt atcctaattc accgggtttg tttgtttgta taacggcacc acagccgata
    14941 cattgtaatt cttctgtcat tcgattccac ctcgccagcc catatcaggg tgttttgcta
    15001 atagataggc cataattctt ttttcccaaa aacgattaaa ttgtgtcttc caactatctg
    15061 ttgcgataat gggttttaca aggatgctac gtatccctgc acgattggct ccacgtatat
    15121 cagtcataat ctgatcgcct accattacta tctcatcatc tgctaaatcc aattgtttct
    15181 ttgctatgtt gatccctctt gctaaaggtt tcattgctcg tgcaacatac aaaagatcaa
    15241 atttttcaat cgctcgcgcg acacgttttg aattattgtt tgaaacaaca acaacaggga
    15301 ttcctgcatt tttcatttct aaaatccaag taagcaactc ttctgttcca tctggattat
    15361 tccatgcaat caacgtattg tctaaatcgg tcaatactgc tttgatgcct agattcttta
    15421 attgggcagg tgtaatctga tagatggctt ctaccatcca tgttggtttg taatttgaaa
    15481 acatgctgtc tcctcatctg caaaaaatga aggagcacat aaagtactcc tcaaccccgt
    15541 attatactgc atatcagcaa aaaaaactac ctctatactc gttcgtcttt cagaaaaagc
    15601 atttctttac aaaatgacca aaaataaaga ttgatcacag aaaaatttag aaacaaaaaa
    15661 caactaatat tggcggaacc tcttgtatcg tttttccagt aatttatgct gatctaatcg
    15721 aacaagttcc gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg
    15781 tggcaactgc tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg
    15841 tagttctggc gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt
    15901 ccacaaaatc gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat
    15961 ccagacttca tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat
    16021 gatggcgatg atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc
    16081 tccttcgccg cgctcttctg ctcctacacc gcaatatgca cctgctgtat tgatcaaggt
    16141 aatcaccggt ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc
    16201 ttcaggatgg ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg
    16261 aataccgatc actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc
    16321 gtcatctgca aataaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa
    16381 aaaatccaag cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt
    16441 cttatccacg atctatccat cctttctgtg tatgcaattc aagaagcgta tggattttct
    16501 gtttcaattc catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag
    16561 ccttttggaa atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag
    16621 caaaaccgat caagctttgg ggttctgcta gaataatatc gccttccatt gcaaaactgg
    16681 cagttactcc tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc
    16741 tatgccgttt gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt
    16801 gcatccgtgc gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc
    16861 gttcgaataa tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat
    16921 taggatccat cactccaata atgaagggaa tcccctgaat ggttgcttta ccagtgagga
    16981 ctgcttcatg aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa
    17041 atgctagcgg atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa
    17101 taatcaaagc tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata
    17161 ctttttcttc accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc
    17221 acatgttatc aggaacagat ggcgagttag caggtttgct ctctctatta gggttgatcc
    17281 gaatatattt ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg
    17341 tccaattcgg taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg
    17401 caggatgact aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa
    17461 attcacttaa ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga
    17521 ttatttttgc aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac
    17581 tgtctactcg cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag
    17641 aaggagcaaa atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt
    17701 ggaatactac ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt
    17761 tttttactaa atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag
    17821 tattcatttc catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg
    17881 cattttcata attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt
    17941 ttgtttgtga gatcactaca gaaggagatt cttccagtac tttttgattg ttgcgttgta
    18001 aagaacaatc tcgctcgcct aaatgaatga catgaccaaa tcgatcacct aagatctgaa
    18061 cttctatatg gcgagcagga taaatgattt tttccaaata catatcatca tttccaaatg
    18121 ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa
    18181 ctttccgaat gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa
    18241 ttctgtctgc tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa
    18301 tgactgggac atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat
    18361 cgatcgttgc acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa
    18421 atcggctatt ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga
    18481 ttgcagcgct cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa
    18541 tgcagattgc ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata
    18601 tcgctacggt ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac
    18661 cgcggttagc tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat
    18721 tccgcttcgg ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa
    18781 gctttcactt tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta
    18841 cggaattttg ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga
    18901 gaaagtaatg caactgctcc tgcttgagcc attgcttcaa caatcaatac ccctggcata
    18961 acaggttctt ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta
    19021 gcaacgatcc gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa
    19081 cgatgtggaa tgatttcttt tatttcttgt atgttcattg ttttccctct ttcctgcttt
    19141 gcttattttt gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct
    19201 acaaggattt ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc
    19261 gcttcaataa tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct
    19321 ggtttgtcgg gcgctggttg cagataaacg attcccacga ttggcgaaac aacctcgatt
    19381 ccatcaaata ctgccgattc tttttctttc attatttctg gagcagttgt tgcttgttca
    19441 gatatgacat tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg
    19501 cctcgacttg ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat
    19561 tccgttagcg aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa
    19621 attattcctc ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat
    19681 tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa
    19741 tctcaggatc ttgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca
    19801 gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt
    19861 tcgtgctgct gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg
    19921 cttctgcctg gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt
    19981 ccggctgtac accagcttct tccatcgcaa gcttcattgc tttagatgca ccagaaccat
    20041 ctggagtagg agctgtcata tggtaagcgt cacagtttgc accgtatcca acgatttctc
    20101 catagatttt ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacgcctg
    20161 ctccttcacc catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa ccacgattag
    20221 gatcagttga ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa
    20281 tcgttgcttc tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga
    20341 atgcttcccc aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac
    20401 cttttgcacc tacacgcaaa gcaatatttc cagctgccat gttggcaatc gccattggta
    20461 cgaacattgg agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga
    20521 ttgttggaag accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt
    20581 tttctttatc taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg
    20641 aaaacatatc cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt
    20701 tgacttcacc cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga
    20761 tgccattcgt cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg
    20821 aagtgactcc atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc
    20881 cgtgcatgac caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg
    20941 ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt
    21001 gcttttccgc ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga
    21061 atcctggcgc aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt
    21121 ttgttaatcc tactacgcca gctttactag ctgcataatt tgcttgacca atatttccaa
    21181 tcaacccaga tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt
    21241 tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt gatatccaga cattttttga
    21301 aatcttctgc atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta
    21361 acacatggat cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat
    21421 aatcagagat gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta
    21481 tcttttcttt tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa
    21541 aagctaaggc catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc
    21601 cttttacatc cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt
    21661 attctcaaca cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac
    21721 ttttccgggg ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact
    21781 ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt
    21841 cttcattatt tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat
    21901 ctgacttagg tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg
    21961 cccgctgaca tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc
    22021 ttcgtctact gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc
    22081 aggagatacg atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg
    22141 cgcattcatc actgcaacca ttttcccgct tccagctggg gtagcttctg tcatataagc
    22201 gcctcttttt gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa
    22261 tgccgaatat tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt
    22321 caataagcga taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg
    22381 atccaaacgt tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact
    22441 tgcttcatca aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg
    22501 atattgtgca ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc
    22561 cagaatatat gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat
    22621 gtcccagccc ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta
    22681 aatcttgaac cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa
    22741 tttcattgat gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag
    22801 tagtcaattt gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt
    22861 tttcgaatac cataataata tattcctccg atattgtttg ctttttattt ttataattta
    22921 acagtctaca actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat
    22981 attactttct gctgttttcc taaggtgatc tttccactag aaactgcttc atccaataag
    23041 atagggatgc ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct
    23101 tgcggcatct ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa
    23161 agcagataat caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt
    23221 acggcaaaat caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct
    23281 tctgaataga acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta
    23341 ccgtcagctg ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc
    23401 ccacctgctc catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta
    23461 gatagtgttt caccaccaat caccagtccc ttagttgagc cgcagcggat cattttttca
    23521 gccatgctta acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca
    23581 tgagttgctc ccaatttccc ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc
    23641 gacattgttg ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc
    23701 aatttttccg ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt
    23761 tcacgaatac ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga
    23821 tcattattag taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat
    23881 tgttccatta aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca
    23941 tccagcaagg catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca
    24001 cgatgaaaat gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt
    24061 acaacgcggc ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg
    24121 ttaatagcag tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt
    24181 gtttttttct tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct
    24241 ttgaattgag attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta
    24301 acaagataat catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt
    24361 ttgattatca aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt
    24421 aaaagatcat ttttttacta tatgagcttt ttttaagatt ttactcattt aaaacaactg
    24481 ataaatcagc atttataatt tcttttatta gaaaaatcat ctatctcaca tattttgatt
    24541 atcaaagtat tttctgtgaa cagaactata gaattcccta aaacttgtcc attatttcaa
    24601 aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat
    24661 gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgagatg gtaaggtcat
    24721 ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa
    24781 acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt
    24841 agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc
    24901 tttttataac cgacaaatga attatttcag taattataat tatgtacata ttaccaataa
    24961 gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa
    25021 aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga
    25081 aattagtttg ttagctttgc gagtccagct tcctgaccta caggaaatga cttttgaaca
    25141 gaaaaaaaag gaactccaag ggctgtttca aggccgaaat aaaaagctac tgacttactt
    25201 gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca
    25261 aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa agcagttttt
    25321 aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca
    25381 ctacctcgtt tgttatggtc aacttgcaga aacaatgatt acctctggtg acgtttctcc
    25441 tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga
    25501 agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtt
    25561 tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg
    25621 cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt ggattaagtc
    25681 atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat gagtatctgt
    25741 ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact tctctacgcc
    25801 ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca tagcctccag
    25861 tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc
    25921 tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa aaactcgttt
    25981 tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc aaagcttggg
    26041 ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat gcttgggtag
    26101 ttgctggcaa tacaagatcc attttttctg cttcttctaa tgcaatcttt aaatctttga
    26161 taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga ggagaataat
    26221 tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact ttctttaaat
    26281 caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc gtacctgcaa
    26341 tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca tgcaatgtaa
    26401 atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct tcttctccac
    26461 cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat accggtgcat
    26521 ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc aggcttggtg
    26581 tacttgtagt caaatcaact aaaatatgtc ctgagatatc tgcagaaaaa atccccgact
    26641 gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag agaatattac
    26701 tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt actaaaggat
    26761 cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct agtagatggc
    26821 gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt ttttcgacct
    26881 cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag gattttttca
    26941 taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat tcgttcaatc
    27001 gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat gtcttgataa
    27061 aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt agcttctaaa
    27121 acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg tgcaaagtct
    27181 tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga acaatcggtt caaataagca
    27241 tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt aagcccttgt
    27301 gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag tgctacatca
    27361 ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc tgagatcaat
    27421 tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc gttaggataa
    27481 gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttctc ataagaaatc
    27541 ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc ccttaatttg
    27601 ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat accagtagga
    27661 atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg tatcaataac acgttcactt
    27721 ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt tacatgggat
    27781 tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat cgtatggtaa
    27841 gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat ccctgctccg
    27901 aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa ataagcatac
    27961 cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac acttggcatt
    28021 cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa aatatagact
    28081 tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc aactccgctg
    28141 acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta
    28201 gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca gtacactttc
    28261 gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg ctgtttgata
    28321 accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca gtcggataaa
    28381 ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc cattatacac
    28441 tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa caacgatccc
    28501 ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg caccttctat
    28561 gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt cttttacaat
    28621 tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa taaatgtgta
    28681 ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa
    28741 accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc tgaacttttg
    28801 ttctttcatt ggatcagggt gatatttctc taagtcgatc ccattggaaa tagcttgaat
    28861 cggcacacgg ataccatagc tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag
    28921 atagtccgct ttttggtaca atcctaccag atattttttt acgataggag aaagctggtt
    28981 cgatccgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt
    29041 ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt atgtattgat
    29101 gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga cttccgtcag
    29161 agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca tcttttcact
    29221 ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt ataacatagc
    29281 ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga caaaaacgtt
    29341 ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc agcttttgat
    29401 acgttattta ttttacatat tcatgaacaa gttcaacgac ttcttccatc atatcgcatt
    29461 ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt ttcattaaac
    29521 tacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc atacctacta
    29581 gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc cattttcctt
    29641 cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg ttatatggtt
    29701 ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat
    29761 cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct aagactgctg
    29821 ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc ttgccttcac
    29881 ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg
    29941 tagctaccat agggaacatg atacgtaaat taccatgaac agatgcacga agcaatgcac
    30001 gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc aatgcacgat
    30061 atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct ttatctccac
    30121 cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat acagctttgt
    30181 aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac aggaattcag
    30241 tacggtacag accaactgct tctccaccat tattatggac accaactaga tcttttggtg
    30301 ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt
    30361 ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt tcaaattcag
    30421 ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg acagccaaaa
    30481 tatcgccttc tttgacttta gcagtaattt cttttgtccc aacgattgct ggaatttcta
    30541 aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt
    30601 ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt gcaacaacga
    30661 ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag tgcgctaaaa
    30721 tacgtttagc aacgtcacga atatctgcag cacgctcttg catataagca ttatcttcca
    30781 ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat tcagcattta
    30841 cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct gaaagaacca
    30901 tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct gctttttcac
    30961 gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga gcttcttcag
    31021 ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact agcaggtaag
    31081 cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca accattattc
    31141 agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag cttcgtcagc
    31201 accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca taacgcccat
    31261 gattgatttc aagtttactg atttaccttt atactctaag tttacatcag agttaaattt
    31321 gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt ctgctactac
    31381 gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg tttttatgtg
    31441 agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt catctgtaaa
    31501 gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat aaaagtattc
    31561 aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaac gtgtttattt
    31621 cctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt gaccctcttt
    31681 gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat aagagtcaaa
    31741 ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta
    31801 ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt
    31861 gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca
    31921 cttgtatgcc actatcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca
    31981 aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg
    32041 atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa
    32101 ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc
    32161 acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc
    32221 acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga
    32281 aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa
    32341 aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt
    32401 aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg
    32461 aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt
    32521 gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa
    32581 tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg
    32641 tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat
    32701 gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt
    32761 gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc
    32821 cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt
    32881 gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat
    32941 cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt
    33001 cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca
    33061 gcttactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca
    33121 acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt
    33181 agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa
    33241 agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt
    33301 atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact
    33361 attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag
    33421 tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt
    33481 aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc
    33541 tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc
    33601 acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac
    33661 tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt
    33721 attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat
    33781 cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga
    33841 tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga
    33901 aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat
    33961 ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga
    34021 gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt
    34081 ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta
    34141 tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta
    34201 taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc
    34261 catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa
    34321 cactgatgcc catctagtca aggtctacac agccggaaat accactattg tctacactga
    34381 agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga
    34441 aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga
    34501 tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt
    34561 aggcgctaca ttagccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa
    34621 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcgaaa gcagaagcaa
    34681 ctccttcgga aataagctga aattcacgaa aattgctttt caaatttttg tgaattccag
    34741 cttatttctt ggagttaaac gcttctgtct cagcctcttt ttctttctag ctttttgatt
    34801 tttctctttt cttttgtact tttctctttt cattttcagt tctacgcgtg tctttatttt
    34861 taactttttc ctctttatgg atgattgatc gtactttttg tgcaatttgc tcgtttttgt
    34921 cccagtcttt tacatctaat aatcgacata atcgtaacat ctcttctttt ttctgttttt
    34981 ttctttcacg tctttgccat tctgtttcca tgtgtcacac tctcctttct ttaatcatat
    35041 gtacgcaaaa taatttttat atttttttga ttagacattt ctatgatttt aatatagtaa
    35101 aaagagccgt cacggaaaat ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt
    35161 ttggctattc ttattacttg atgcagggca gcttgtgtca agacttcttt acatcaaact
    35221 ttttaatttt acattgggat atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa
    35281 taagaacaac ggttggtcga aacggtcacg agccaagata ttgcgacttg aactcatctt
    35341 ctcatccaaa tcttctggat cgatccagcg agcgattttg tgtcccattg gagacatgat
    35401 tacttcggcg ttatactcat ttaacatacg atattggaat acttcgaatt gtagctgacc
    35461 aacagctcca atgatatatt cttccgttag atatgtttta taaagctgaa tcgctccttc
    35521 ttgaactaat tgatagattc ctttgtggaa ggatttttgt ttcatgacat tttttgcagt
    35581 cactttcata aacagctctg gagtaaatga agggagttct tcataagcaa cttttagttt
    35641 tccttcgaac aatgtatccc caatctgata attgcctgta tcatatacac cgatgatatc
    35701 gcctgctact gctttttcga cgttttctct cgaatctgcc ataaattgtg tgacattgct
    35761 taatttcagt tttttatttg tacgttccaa ccagacatcc attcctcgtt cgaatgttcc
    35821 agaacagatt cggacaaaag cgattcggtc acgatgggct ggattcatat ttgcttggat
    35881 cttgaatacg aaacctgaaa attcttcctc ataaggactt accatctgat catcttgtgt
    35941 tttgtgtcca taaggagctg gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc
    36001 gaagttagtc aatgcagaac caaaaaatac tggtgtctga tcgccacgag caattttttc
    36061 ttgattaaat gcatctccag cctctttgat tagttctact tcttctaaga cttgttcata
    36121 taaggaattt tcagttagag gatgattttc gggaagcagt ccatcttcat ccaattctac
    36181 taatcgttca tcctgataat tttccggacg ataaaactct acacgattat ggtagatatc
    36241 ataaagacct tctagccctt tacccatacc gattggccaa ttcatcggat aagattcgat
    36301 atctaatagc tcttctaatt cttctaacaa ttccaatggt tcacgaccat cacgatctag
    36361 tttgttgata aaagtaaata tcgggatgcc acgttttttt acaacttgga ataatttctt
    36421 tgtctgtgct tcgatccctt ttgcactatc gattaccatg accgcactat ccacggccat
    36481 caaagtacga taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca agatatttac
    36541 tcgtttccct tgataatcaa actgcataac agaactagta acagaaatcc cacgttgttt
    36601 ctcgatttcc atccagtctg atttggcaaa attacctgtt tttttgcctt ttacggtccc
    36661 ggcttgacgg atagccccac caaacaatag taattgttct gtaatcgttg ttttcccagc
    36721 atccggatgg gaaatgatcg caaaagttct gcgattgtcc acttgttgct ttaattttgg
    36781 attattcata acctaactcc atttcttcca ttcaattatt tatattttct ctaggcacag
    36841 attgtccgat ctcttaaaga aagacaattt catatagggc tgtgacaaaa gccgtgtagc
    36901 agtctctttt tccgagtaaa cggcgttcta gagacagcct atcagcaata agcccaccca
    36961 agccaaaaat acgaaaagct gtttttgctt ggctgctctt attacttaat gcaggatagc
    37021 tttttcacaa cctttgtcaa ctactacaac tgaatcagta tagcatggat tcagcgaatg
    37081 aaaaagccac caaaccataa gagtcaaata tttttttgtc agctgactaa aagaaaagac
    37141 gtgttgcttg tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg
    37201 gctcgtcatc ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg
    37261 tttcttccgg atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt
    37321 cagaaaccag cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca
    37381 tgcctaatgc tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac
    37441 tgtcaaaagc ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt
    37501 catcgatttt ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga
    37561 tttcttctaa aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata
    37621 aaatagccat ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg
    37681 tttcaggaac gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga
    37741 atttatgggc agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt
    37801 cttcgttata aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt
    37861 ccacagtgtc gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg
    37921 caactttcgt atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata
    37981 cgccttcgtt ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat
    38041 caaaagtcat cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga
    38101 agggcttagc tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt
    38161 ctgatttatt taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg
    38221 tcaaaatagc cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata
    38281 ccggagctaa ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca
    38341 aagtgattcc tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa
    38401 gcttttgaga tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg
    38461 aaacaacagc aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata
    38521 ataaaatctg cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc
    38581 cttataaaat taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa
    38641 atggtatcac aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag
    38701 gtcgtagcaa ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata
    38761 taggaaatga tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat
    38821 ccggcaatat ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt
    38881 gatttcccaa ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca
    38941 accacacaac aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt
    39001 gctagtccta gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca
    39061 aataagaaag cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg
    39121 aataaacatt cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc
    39181 aataactcca caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact
    39241 agtttttctc cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg
    39301 gttgccgaag cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat
    39361 tgctgaatga ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact
    39421 tccttgaaca aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa
    39481 tcaggcatat tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt
    39541 aaaatccaaa taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt
    39601 ttgatttttg ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct
    39661 gcaatcagta caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag
    39721 tttatttctg atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac
    39781 attagtttag actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt
    39841 atatattata ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac
    39901 tttttatttg ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag
    39961 ttgtacaaac aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc
    40021 aagtttttgt agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc
    40081 attgtatcca cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt
    40141 tgcctttaga aaacggaaaa atgaaattac agcggatggc gattctttct gaatacagac
    40201 accaagggtt aggaaaaata ttgattgaag aagcagaaaa ctttgctaag aatcaaggct
    40261 ataatacgat tttattaggg gcacagtcca cagctgaaac tttttacgaa aaactaggat
    40321 atacagctta cggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga
    40381 ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc
    40441 actggcttac tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc
    40501 aaatatcaat atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa
    40561 tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc attttgctgt
    40621 gcatctcata ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat
    40681 ccaagtcata gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat
    40741 tacaataata ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt
    40801 tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga
    40861 ctaatgtgtg atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat
    40921 ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc
    40981 cttcttttgg aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg
    41041 gtgttcatta caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag
    41101 gattcctatt gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa
    41161 aaacgaccga atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt
    41221 cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac
    41281 taaaaatatg agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt
    41341 caaatgggag attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct
    41401 ttgtttccca ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa
    41461 atgtccacat attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag
    41521 aatgattttg aagagctgca ctgatataat aaacgtcttt tggctttgct ttttttgttt
    41581 ttacaggaaa actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac
    41641 aagtctcaca ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt
    41701 ggttgaattc acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt
    41761 tcctgctaga agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca
    41821 tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc gatacttgca atcgcgcctg
    41881 tcgtatatgg tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc
    41941 catcaaattc tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat
    42001 aaccaaggcc ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa
    42061 ttgtaggaaa ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt
    42121 gctgaagcat gatctcagag atccagatac gataaggatc acgattatag cgccaaggca
    42181 ggtttctttt ttcttgttca taccattgaa tgaactgatc ttgaaattct tttgtttctt
    42241 tatctgtcca tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc
    42301 tggatcatat ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt
    42361 tttttaggtt caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa
    42421 tcatattcta tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg ttggatcact
    42481 tcttgagaaa agcctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg
    42541 ctttttccgt gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg
    42601 tcttcaaatg tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc
    42661 tgcatctgct gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca
    42721 taactttccc catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt
    42781 ttatttcgat cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc
    42841 gatcgcaact gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta
    42901 ctcttcttga tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag
    42961 cgtactaata catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag
    43021 aaagggcctt tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat
    43081 atattctcta tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc
    43141 acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg
    43201 gttcctttga aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg
    43261 ttgatcatat ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa
    43321 gcaactcctc gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc
    43381 gtagtggcac catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct
    43441 tatcaagctc aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa
    43501 ccagcaggct ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat
    43561 tatagaaaca aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct
    43621 ggtttatacg aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa
    43681 cctcgaacac aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc
    43741 atctatgatg aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt
    43801 aaaactgggg agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac
    43861 gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc
    43921 caggcacaaa aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa
    43981 gccattgaag aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa
    44041 ctcaatcctc agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag
    44101 cctaatgaaa cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca
    44161 aaacaagcca aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa
    44221 aaaaatgcgg aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa
    44281 gttctgccta aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca
    44341 cgtacgggtt tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt
    44401 gtttatatct cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag
    44461 ttcaagattg aatatttaca atccgtcgac atgtttcctc agactgcacg ttgtgaagtt
    44521 gtcgtgaaga tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg
    44581 tatcaagtaa taagaacagc caaacaaaaa tagctcttca tgttttatga atactgtact
    44641 tgtgaccgaa gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt
    44701 tttgtcacag ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca
    44761 tttgtcgtat cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc
    44821 ggaagtgtga tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt
    44881 gtgatcaatt caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta
    44941 atgtagtaat cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat
    45001 gttcctggat taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca
    45061 aagccgctga ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg
    45121 atactttgcg gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat
    45181 gttcctaggt atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg
    45241 ctaaaaccga acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc
    45301 atgtagaaac ctccttcatt tgataaccac attgtaaacg ctttattgta ttaacacaaa
    45361 cattatgctt ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt
    45421 ataacagaag tagacgagaa aagtacaaat agaaaacatg agaatcaaat catatgatct
    45481 ttttcaaaaa catcattcat ataaacaaat gttcaacttt ttaataacaa caattatcaa
    45541 tttttttctt acttcacaaa ctaaatacag gtcttgctgt gagccttcat ggtaaactaa
    45601 tattatagaa aggagttagt aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg
    45661 ttataggaat tgtcatatta ggctttttat cagggttgct tagcggaaat cccggaaaat
    45721 attactattc tttgcaactt ccaccatttg caccacctag ctggatattt gggccgatgt
    45781 ggaccttact gtatatattg atgggtattt ctctttattt gttacttaat cacaacaaca
    45841 aaaaacagcg aaacaatctt gtcggtcttt ttgtgattca gttcattttt aattttatat
    45901 ggtctgctct attttttaat ttaagaaata ttttcattgc agcaatcgat attacacttt
    45961 tagttatttt cttgtcagtg cttatgtatc agttgtggct gcatcatcgc ctggcaatgt
    46021 ggctcatgat tccttattat ttatgggtac tattcgcaac tctattgaat tattcaattt
    46081 attttttaaa ctaagcgcta aaaagagacg tcctgtaatt taacacatag aaaaagtgcc
    46141 acctaagtta gattttttgt ctaatttagg tggcactctt tattaaccaa actatttttt
    46201 tctagtaccc acttgttgtt ccatactcat tattctggtc ataaccataa ccattttgtt
    46261 cctgtccagc gtaaggatca taactttgag tatcttgact tggtgtatag tactgacttg
    46321 tatcttctgt ttgctgataa taggtattcg gatcttggta ctgatcatat gtgttttctg
    46381 aataggaaga ataatcggtg tacccttcac tattcgatcc atcaccgttt gtcgaatcgc
    46441 catcttccga atcatcaaag aacatcattg aagcattctc atttttcaaa ttaggtaatg
    46501 tatcgcttgt ttttatattt aattgtttct tcagttcatt ttggatcgaa agaagatcat
    46561 tttttcctaa aatttgatag tagatatcat tgatcatttg tccttcgcct tgcaactggt
    46621 cttgtttgat tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc atgtcatccc
    46681 aatctaagtc cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta taattgctta
    46741 cgccatctaa acttaaaact ttattgacga ttttcgtcac tacttcccgc tgacgacgtt
    46801 gtcgaccgat atcgccttct ggatcttcgt gtcgcattcg agaataggct aaaccttttt
    46861 ctccattcaa tgtttgttca cctactggta catggacacc atccaaagta aagtcaattt
    46921 tattattcac tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca tccaaattga
    46981 tcgttacata atgatcaata ggaatatcga gcagttgctc aattgaatcc atggccattt
    47041 cgacaccacc aaatgcgtag gcatgattta atttatctac cgttccataa cctacgatat
    47101 tcgtataaat atctcggtcc aaactgatga tcgttgattt tttttgtttc ggattaactg
    47161 ttactaccat catcgtatca gaacgtcctt gttccgttct tcctaatcca ccggtatcta
    47221 aacctaatag caaaaccgaa aaaggttctt tgtcatcaat gcttactctc tcagaacgtt
    47281 tggaggatat tcgattagag tttttagaaa ttttgtttac tgtttggctc gcttctccca
    47341 taaatctgat tccgtatgct gataatccgg caatcagcaa aacgaatacc cctaacaagg
    47401 ttaatagaac tttttgccat cgcttcataa tacacctctg tttcacattt tctaaaatat
    47461 tgtctcatat aactgttcaa tcagcaaatt cactttttaa tcataagaaa actttaaaaa
    47521 acatttttgt gtaacgatta ttatttttta aaatttctca aaattcttaa ctatcatacc
    47581 ataccgtgaa aaagatcgaa agaccgaata cacgcagttc gcaatcgttt tatctttttt
    47641 tatgtatttt ttccagaaaa agcgaacaac aaggtatttt ccaaataatc gtgcagtaaa
    47701 atgatcgcac atacctttcc catcttttta acagtctttg cctaccgttc agattatcat
    47761 tcgatttcac gacaatttct tcctatgact ggtccttttg ttaaacccat cttctattcg
    47821 ttttatttcc agctcactgc atggctctta aaatgactcg aactagtgat tgcattacca
    47881 tcatctttta atggatagat atgctcctct tcaaagtaaa acgattttct tttaaagtaa
    47941 tagaattgac atggtaatga gtatgtatac tagtggtttc acaaatggtc ttttattcaa
    48001 catccaaaaa ctgaaatttc tttaaaatcc attactaacg aagaaaatgt tccttgcttg
    48061 ataattagtt aggacatgtc aagtgaacaa atgcatttac ttagccaaaa aatctaaaaa
    48121 cttcataaat agacaaaaag acacgactct ctaagaagtc gtgtctttat tcaacaaatt
    48181 attataattt agtcacttca gcagcttgag gtccgcgtgc accttcaaca atcgtgaatt
    48241 ctacttcttg accttcttct aggctcttga agccgtctcc ttgaattgct gagaaatgaa
    48301 caaatacgtc atctccgccg tctactgtaa taaacccaaa tcctttttca ttgttgaacc
    48361 atttaaccgt tccgtgttcc ataaatgtat atcctccaaa aaagttcttg caagtaacct
    48421 tgcaagaaca ttatacccaa tttagaaaac gctagcaagc tttcgctctt ttattttcca
    48481 aattattttt aagactgata tgacggagga aatggttatt ttgctgcttc gaaacgtttg
    48541 tttacttcat cccaattaat aacattccag aatgctgcaa tataatctgg tcggacattt
    48601 ttatatttca aataataagc atgttcccaa acgtccaaac ctagaattgg tgtctttcct
    48661 tccatcaatg gagaatcttg atttgcagta gaggtaatag ctaattttcc attttccatt
    48721 acaagccaag cccatccaga accaaatcgt ccagcggctg cttttttgaa ttcttcttta
    48781 aaagaagaaa aatcaccaaa agcttcatta atcgcttctt ttatttctcc tgtaggttcg
    48841 ccaccagcat ttggtgccat gatttcccag aaaaatgaat ggttagcatg tccgccacca
    48901 ttgttacgta cagctgtctt gatatctgtt ggaatagcgt ccatatcaga taataattct
    48961 tctattgttt tttcgcctaa ttctgggtat ttctcaattg ctgaatttaa attcgtcaca
    49021 taagtattgt gatgcttatc atgatgcaga tgcatcgttt cttcgtcaat ataaggttcc
    49081 aatgcgtcat atgcataagg tagatctggt aatgtgtaag tcataaaaaa attcctcctc
    49141 aatgttttct taataatgtc tacattacaa agagtaacaa agagaaggaa attcttcaat
    49201 tgttatgcct agtcgtcttt ttcactttga cctacttctt cttcaaaaac ctcagtttct
    49261 ttaaaaataa acaacttgct gaaaatatag ttcgcaagga taacaataat ttgagtgatg
    49321 attttcgcca catactcatt gctgttcata acattgatca acaataccat tgcccccatg
    49381 tccaatccgt aagaaaggat acggtagaag aagaacttac cgcattcaat aagtgcatgt
    49441 gtaaaattcg gtgattttga atggaatacc caaagtttgt tcgttacaaa agcaaacaat
    49501 acagaacaaa accacgaaat agaattggat aaggccatac ttaaatcaaa atattggcgt
    49561 gcgagagcaa aactaacaaa atttacaatc gtagccaaac caccaaaaaa gagatagact
    49621 agtatctccc aatatccttt tgcttctaaa tatcctttta actgtcgata ctgcttcatg
    49681 agccaaccta ccttattaaa aattcttatt tatcatactc tatatttcaa taactttcat
    49741 ctttatttaa aaaaattaat tttttttgat cctttgcctt tgtctctgct tggatcgtca
    49801 catgtgtaat accatatttt aattttaata aagaaattat acgaggatat atcttttcaa
    49861 tctcgcttag tagctgatct tcaaaatcaa tatgtccgtc gaaaataatt atcttttcgc
    49921 tatactgcca aatatggaca tgatgaacat cctttacccc atcgattgct tcaatttctt
    49981 gttccatttt ttcatagtcg atagcaggtg cactttgtat caaaatagcg gcagcttttt
    50041 tgatcaccat tatcgcttct atcaatatat atatcgaaat gatcagtgta ataactggat
    50101 cgatccagta gatatcgaaa aaacggatca aaattgctcc gataatcaca cttaccgaag
    50161 acagtgtatc acttaacaaa tgcaaataac tagattttat attcaaactt tcatctgctt
    50221 cttgtcgtaa taataatact gagaacaaat tcgcgagcaa tccaataact gctactgtta
    50281 gcatcaaatg actatttatc ttttctggtg aagtgaaccg cttgaatgct tcaacggtta
    50341 aaaacaagga taatatgatc aatatgaatg agttgatgaa agcactcaat atttctaaac
    50401 gctggtagcc atacgtacgt tttttatttg cttcttttcg ggccattttt tgtgccccgt
    50461 aagaaaaaat aattgccaat gtatcactaa ggttatggaa agcatctgaa acaagagaca
    50521 aactccctga cataatccct ccaataaatt ctgataaagt gatcaccgca ttcaaaacca
    50581 ctactccaaa tagttttttt gatgatctct gtggctgtgt aggcactatt ccttcctcca
    50641 ttttatttat attggatagt ctttagacaa gaataacatt tttttgatat ttcaccaatc
    50701 attcagctgt ttccttaaag cacttgtatt tcacttgata cttcgctaga attgcaagtg
    50761 atgacactac tagatgagga gtacttatga caacaaaaca attaattatc agttccttta
    50821 tacgattcaa agacctaaag gaggcgagaa atgtcccatt ttggaaagcc attgtctatc
    50881 ttttagtttt gagtataatt atggctttgc caatctcttt tcaagttttt caagtcttaa
    50941 acaacattca attggatggg caaaagattg ctactcgcat tcctgatttt acaatccaaa
    51001 acgggcagct tcaaaccgaa gaaaaagaag gattcattta ccaaacaaac tccattatct
    51061 tcacatttga tccagaggga aaacgaacag aacaggacat ctctactgat ttgatgggga
    51121 attttgttag tgtcgggatg ttaaaaaaca aacttatcat agctcttcct aatacaggca
    51181 cgacttctgc tttgttgaac aataaccaat tagaacttcc ctacaccaat gaatctttaa
    51241 aaaacttaac aggtaagcag ctccgttctt ttttaagtga agcatctata ccactttgga
    51301 taaaagtcct gacatttata ttttctattt atcctagttt tctgaatctg ctgatcactt
    51361 tgctatttac caacattgct gcttttctct atgcacgctt tcgactaaca aaagctactt
    51421 ttcttgattg tttaaaaaca ttgatctatt caatttcatt gcctactata attgcaacaa
    51481 tcttgatggt ctttcttcct tcttttgaca ctagcgcctt cattgctttt gcaggaatct
    51541 ttatttttgc tcaagctgtc aaaggatggg caaaaatatc gatcaattaa gaaataaaga
    51601 caactcgtta tttctacttc aatagaccct tttagaggtt gtgacatttt tttgtcacag
    51661 cccctttttt atttgaaaaa tatttctttc tcaaaaaaca agcaaggcgc ttcaaagttc
    51721 ttcttgtatg cgctacactt agagtaaaga aaggcgggaa attaactatg tcagaaagaa
    51781 tcatttatgc agatcgtcca tttcgtgatg aatttatcga agaaatccat actatagcgc
    51841 ctggttatat ctttaaaaca gaattacaac cagaggatct acaaaatgta gagatcagtt
    51901 tgggctggaa ctcagactac caagaaaaac tattagcctc ttctaccttg aaatgggtcc
    51961 aatctatctc agctggtgtt gatcagctac cccttgagac tttttcaaag aaacatattt
    52021 tactttcaaa tggtagtggc atccacagtg aatcgattac ggagcatgtc ttaggaatca
    52081 tccttgggta ttctcgtggt ctttttcaag cacaaaaggc acagagtgac atgaaatggt
    52141 taggttcatc ggtccattac caatctatcc aagggaaaaa aatactaatc attggaactg
    52201 gccatattgg aaaaatgatt gcaaaaaaat gcaaagcatt agacatgaac tgctatggaa
    52261 tcaatacatc cggtcatcca gcagaaggaa tggaaaagac tttttcttta aatcaactaa
    52321 aggaagttct tcctgaagta gatttcgtag tcaatatctt acctctaacg gaggaaacaa
    52381 ctgggctctt tgatcagcat atgtttgaac actttgatcc aaaatctgta ttcatcaatg
    52441 ttggccgtgg tgcttctgtt aagacacaag atctggtgca ggctttaaat aaccagcaac
    52501 tttcctttgc agcattagac gtttttgagg aagagcctct tccacaagat catcctcttt
    52561 ggaaaatgga caatgtactg attacttccc acattgcagg tttgacacct gattttcaaa
    52621 agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc catgagcttc
    52681 aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata ttttcaaaag
    52741 aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt ttttaaagtg
    52801 ataggttggc tcctccaggt tgggatcata atcaacaagt aaatcatatc ctttaaagaa
    52861 ccattcatct gcttcctcga taaaaaaaag tgtcccatcc atttccactt tctttacagg
    52921 atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct ctgttttgcc
    52981 gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt ctgagtcaaa
    53041 ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact ttctcttttc
    53101 tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc caataaccag
    53161 tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa ccgtttgatc
    53221 tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta attcagctaa
    53281 tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat atttatctga
    53341 caactctagg taatctaaaa tattctttga cactaaatta tgcgcaatcc ttattcccat
    53401 gtctcgctcg ggatggacga cgcgatcagc tccgatcttt tcaagtactc tggcatgata
    53461 ctcattttgg gcttttgcta aaatatttgg tacgcccatt tcctttacca tcagagtaac
    53521 taagatactg gcttgaatat cttctccaat agcgacaatt acatgatcga aatttcttat
    53581 gcctaatgat cgaagtgtca tttcatcctg tgcatttgcc acaacagcat gagtggctat
    53641 attcatatat tcattgaccc gatcttcatt actatcaata gccagtactt cttgaccagc
    53701 ttcgatcagt gttcggcaga tacttccgcc aaatcgtcca agacctacaa ttgcaaaatt
    53761 ttgtttcacc gttttcctcc tttagtatct tcttttctca gtataacaaa gcctttacaa
    53821 aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt attttctcag
    53881 cttttgatta tcctctttat catcgttttt atttcaccaa accatgtttg aatgcataga
    53941 tagcggcttg tgtacggtct tccacttcta gcttagccaa tatattggaa acatgtgttt
    54001 ttactgtttt cagtgtaata aacaattcgt ccgctatttc ctgattactt tttccttcag
    54061 aaatcagcat taaaatttca ttttcccgat tcgttagttc ttcatgtaat acaggctcat
    54121 ttcgccgact cattttttcc atcatttttg ttgtcacttc tggttctaat actcgctcgc
    54181 cacgttgcgt tgcccttata gcatcagcaa tttcatgtgc ggtagaggtc ttcaacaaat
    54241 accctgctgc acctgcttca atggcaggat aaactttttc gtcatcaata aaactggtta
    54301 cgataatgat tttagcttct ggccaatctt ttaagattgc ttttgttgat tcgataccat
    54361 ccatttcttc catgactaag tccattaaaa tgacgtccgg tcgaagtgtc atcgccttct
    54421 catagccttg tcgtccattt tcagcttctc caattacttc aatgtcttct tgaatagata
    54481 aataagaaga gacgcctaag cgcaccattt catggtcatc tactaataaa acttttatca
    54541 tccttatcta tgcctccttc atcaaaggga ccttgatttc tacacttgta ccttgacctt
    54601 taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg atattgttca
    54661 atccataact tcccgttttc gtttcattca tattaaaccc tgttccgtcg tcaataatac
    54721 gtaacaaaag attattgtct attttgtgca aatatacctc taattcattg gctttagcat
    54781 gtcttaatgt atttgataac aattcttgaa cgattcggaa caagtgatcc tcaatggaac
    54841 tagatagttt tacatcttct acatcccatt tcagcgaaat ctggatttta ttttgcaatt
    54901 ccttcaataa ctgctcgata ccttgcttta aacttttctc ttctaaattg actggacgta
    54961 gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct gctaccattt
    55021 tcaactgctt gcgaaacatt tctggtgtct cgcttttttc tgcttgctcc gtcaatgccg
    55081 acatcatcat cattgctgca aacaattgtt gcgaaactga atcatgcagc tcacgggcta
    55141 atcgatgtct ttctaattct aaaatttctt ctttggtttg tccatctaca tattggggac
    55201 ggctcgttac aatttgtaat tcactagata cttcaatcat tttttctttt atcttagtga
    55261 tttccttatc aatatctttg atgtataaat cgtccgatgc acttgggatc ggaagaagca
    55321 acaatttgga ttcataattg ccagaagaaa gtgcacgcag cttttcttcg attttcccgt
    55381 attttgtctt ttgaataagt gataataata gaaagacaat taatcctaca cctaaggaaa
    55441 tggctaacaa atggaagata agcggaacat ataataaacg tgcacgcagt aattctcccc
    55501 accaatgact ttgattgctg gcataaaaat aagtgaaaag tgaaaataga ataataagga
    55561 aagccgcaat ccccgaataa acagctagca tcgctctgga tatttttccc atcatattcg
    55621 tatcacctcg acatctccaa tcaaggtatt agtaataatc ttcaatctac gagggttttc
    55681 atcataatca ctgctgtaga ttttgatttg ttcatttttc aaagaaaatt gctcttcttc
    55741 aaacaagaca tttcctacta atgtcgcatg ctctaatcgg atggctacgc ccaaaggtac
    55801 taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat cttttggcaa
    55861 aagcgtattg cccaaatcta tgatagtatc accagaaatg acggcgatgt tgatatcgtc
    55921 ccattcatag acgtcgtttc caatacgttg gttaccgaat aattgctgtc ttttccgctc
    55981 attattatgt gtttttaatt gctctgtttg gatcatcatg atctgtttct ttcgccaaaa
    56041 agcatttttg gttaaatcta tccctgaaat ctctacacct ttcagaccaa taaataaaac
    56101 ggcaaataca gccatcatcc ataaagcagg actgttgact aagctaaaaa aaatgaccaa
    56161 acttcctaat atcaattgaa aattttgaaa tttagtacga ggatgtttgc gcatagctag
    56221 atatatattg aaaataccga atatcactaa aagcaaaagt ccagtattgt ttacgatttg
    56281 ccaaacagca aataacagca gtagtgcttc aactaccaca aaaaaacgcc aagaactatt
    56341 catcgctctt tctccctcct gacacattct catttcatca tacctatttt tttaactgtt
    56401 ggctatagga ctttagatgt aagatcgttt gttgaaatct aaaatacaca aagagaaaaa
    56461 gatctagagt gtatcatcag taagatgaac tctctagatc tttcagtcag actacgcttg
    56521 ttctactttg acgattttaa ctaacatatc gccacctgga gtagcaatag ttacttcatc
    56581 atttttacgt ttgccaatca atgcttgcgc aataggagaa tcattggaaa ttttgccaga
    56641 aaacggatct gcttcagcgc ttccgacaat cgtgtactct tcttcttctc catctggtag
    56701 ttcgatgaat gtaactgttt taccaattga tacctcatct gcatctacac cgtcgttatc
    56761 gatgatttga gcaaaacgaa tcatattttc aattgttgtg attcttcctt caacaaatgc
    56821 ttgttcatct ttcgctgatt catattcaga gttttcggaa agatctccga aaccgcgggc
    56881 aattttgatt cgttcaatga tttctccgcg tttgactgtt tttagttctt ctaattcttt
    56941 ttctaatttt tctttacctt ctagagtcat tggataaact ttctctacca tgtgtaacgt
    57001 ctcctttttt cgataaaata ccgcaaaagg taatcttttg aggaatatag tccaaaaaag
    57061 attaccacgc ttttcttcaa atgtaaatgt ttttctaaaa atgatcaatg tctagagcct
    57121 gtttttctaa gaattattta catatttttc cactaattcc atatgttctt cataagtttt
    57181 agcgaaataa acatttccag tgctcgtatc tgctacgaaa taataatagt cattttctgc
    57241 tggttctaat actgccttga ttgcctcttc gcttggattg tcaaaaggtc caggtccata
    57301 gccggtattg gtatacaaat tatatggtga atctacagct gtatcttcat acgtcactaa
    57361 ttctttgtgt tcacctaatg cgtataaaac agagatatct gactgaagtg gcatatttgc
    57421 tttgattcgg ttaaagaaga cttgtgcaat gtttttacga tcattttctt ttacgccttc
    57481 tttttcaact agagaagcta gagttaaaac ttcttgtaca gtcaaatttt tctgcttgat
    57541 agctgaataa tagttttgca tgaccgtatt cgtcttgttc accatttcaa tcactagatc
    57601 cttaagcgaa gtactcgtgt aataatcata tgtggctggg aataagtaac cttccaaacg
    57661 gtattttgtg ttctcagctt tagatgcact ttccaacagt tctgggaatt tttggtgcaa
    57721 ctcgttaaag aaagtctcat cattcactaa atctaaaaat tctttcttgt ctttaccagt
    57781 taccttcgct acccgttcag caatctgatc gatatcgtac ccttctggaa tggcaatttt
    57841 cccatctgca acttttgttg gctcagaagt accgccttct tgcaactgtt tgccaatctc
    57901 gtcaagagtc atattaggcg ctaactgata atagccagct tgaaagccag ttaaattttt
    57961 aaattttgta taataattga aaacaatccc gcttttaata atattgtcct tctccaaaat
    58021 ctcaccaatc tgcttgttag aagagccact cgggatctcg acttggacta attggtcatc
    58081 tgatttatct aaaggcttca acccgctatc tacataacga tacacggtaa atccaagaaa
    58141 tccgccaata atcaataatg taagagcaat cactaaaatg atcttcctca caatcttatc
    58201 ttctttttta cgcagatttc tccgcttgtc gattatatct gctgtagctg ccttactggc
    58261 ttctttttta tctttacttg ggtgttgttt gtttcctgac aaaacaactc ctccttacta
    58321 tcaagttcgt aactttattc attatacatg atatttgaat tggcgtgaat gattttattt
    58381 aaatattcta tcaaaaatgt aagtagtaaa tccattacta taagattaaa ataaatgtta
    58441 tcaagataca agctatttta gctttattct ttctgtaaaa aagaaaggag actaaataaa
    58501 ctattatgaa aaaaggaaaa catgctaaaa aaagaaaata ttcattttta atgatcgttg
    58561 tcgtgttgtt tagccttagt gcgttctcta caggaactat tcttggatgg caattttaca
    58621 ataatgaaca ctctcagata gttgcccatg ctcaatcagc tcaaaaaaag ttgatactga
    58681 ctaccaatga acaacagctc gttgaaaaaa tcgatcaact attaagaaaa aatgattata
    58741 ttggctcaat ttacgtaaag aaaaataatc gcatcatcat ggaaaaaggc tatggctatg
    58801 cgaataaact cacgcaacaa ccaaatgatc cttctcttta ttatcagatt ggttcggttc
    58861 aaaaagcatt gactactctt ttgattttaa aacaagtcga gcgtgggcac ctttctttag
    58921 atactaagct ggctcaattc tatccacaaa ttgctggcag ccagcaaatt actatccgtg
    58981 acttgctgta tatgcgctca ggcttgcacc gaactgcttc tccaaccata ccgatgactg
    59041 atgaagaagt cgttcaattt gcgattcagc acttaaaatt tacagattat cagacctctc
    59101 attatgattc attgaacttt accatattga ctggaatttt gatcaaatta acaaatcaac
    59161 cgtatgaaaa attattgacc gatgatatca tcgaaccttt aggattacag catacaaaat
    59221 tttatgacca agtcaaaaat tcatccaatc atgcactatc ctatcaaatg tctaaagagg
    59281 atgattactt taaaccactg acagaatctg aaacagagat ccataacgaa ttagggactg
    59341 gtaatatcag tatgtccgtc tatgatttaa atacgctgtt tacacaagtt ttatctggaa
    59401 aactagtttc caaagactta ctcttctcat tatggcgtga aaatgcgaat ggacatccat
    59461 atagtggtgg ggtatatagc ggagataatt atatcctgtc gcaaggaaat atcgactctt
    59521 ttgatacatc tgctatattt agcaaagatt cacaggatgc tatcatcatg gaaagcaata
    59581 taaaagctga caaaactatc aaactgccta ctgctgatct aagaaaccaa atctatgacc
    59641 tgatggaagg aactactaag ttggcaaata gctgatatga tgaaaagata gcaaatacta
    59701 cttctttgaa tagcatttgc ttaaaaaaag tactcgacta aacgtaggaa agagtgtggg
    59761 acaaatctaa aaatcaattt ttgctacaga ctttaattcc aaataaacag aacagaaaga
    59821 ggtgttcttc tttctgttct gtccaaatta ttctaagatt tctctttacc aacattattt
    59881 gacaaagggc ctattaaatc taaaatagta tctttagatt gctaaaatat gtgctagcaa
    59941 atcattcatg gcttgttctt atttttaatt ttatgcaata aaaaagaaga acaattagcg
    60001 tgattataga aataaataat gagattttag aaaaaaatgt gtatgaatac ctgattgtta
    60061 ctttatttat tccactttca ctttttactg taacaaaagg acctccttgt tttagtactg
    60121 tagcattttt tgaattaaca gtaaccgtta atcctttata ccggaataca ggtaaaacta
    60181 ttgttttgcc ttttgaagat ttaaacgtaa atgtaatttc atctttattt ggagaaaagc
    60241 tactataaat aacttgacca tcttctgtaa cttgatggtt tttcaaaagc aattgatctt
    60301 ttttatagcc tataggtaca taatcaaaat tctcaaattc ttcaagttta ttttccattt
    60361 gttcattaga ataagttcct tgaaaattca tttctttcac catgttaact actgatgaat
    60421 aatttaaaat tgaaatagct aatattaata atagtataaa atttcttctc cttgtataag
    60481 aaaatttttc caactgtgga gaaataaata gagatactga aaaagatatg aacaatgttg
    60541 aaaatatcat aaaacgccaa ggagctt
//
LOCUS       DAJSVF010000007        57674 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSVF010000007 DAJSVF010000000
VERSION     DAJSVF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.007"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..391
                     /locus_tag="ON550_000414"
                     /pseudo
     CDS             1..391
                     /locus_tag="ON550_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            391..1320
                     /locus_tag="ON550_000415"
     CDS             391..1320
                     /locus_tag="ON550_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3953528.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1317..3053
                     /locus_tag="ON550_000416"
     CDS             1317..3053
                     /locus_tag="ON550_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3953529.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3116..3952)
                     /gene="pheA"
                     /locus_tag="ON550_000417"
     CDS             complement(3116..3952)
                     /gene="pheA"
                     /locus_tag="ON550_000417"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR3953530.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPACLRALFRKQV
                     DLAVVPVENSLEGSVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIIGDRKMTSQQPAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(3949..4458)
                     /locus_tag="ON550_000418"
     CDS             complement(3949..4458)
                     /locus_tag="ON550_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR3953531.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQRLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4451..5746)
                     /gene="aroA"
                     /locus_tag="ON550_000419"
     CDS             complement(4451..5746)
                     /gene="aroA"
                     /locus_tag="ON550_000419"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3953532.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFMTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5759..6850)
                     /locus_tag="ON550_000420"
     CDS             complement(5759..6850)
                     /locus_tag="ON550_000420"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR3953533.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAI
                     EQQIVDYVANDFETGAEQADVILLAVPVRTTLAYLAVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADDKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(6867..8033)
                     /gene="aroC"
                     /locus_tag="ON550_000421"
     CDS             complement(6867..8033)
                     /gene="aroC"
                     /locus_tag="ON550_000421"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR3953534.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            complement(8035..9099)
                     /gene="aroB"
                     /locus_tag="ON550_000422"
     CDS             complement(8035..9099)
                     /gene="aroB"
                     /locus_tag="ON550_000422"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR3953535.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKNLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9132..10154)
                     /gene="aroF"
                     /locus_tag="ON550_000423"
     CDS             complement(9132..10154)
                     /gene="aroF"
                     /locus_tag="ON550_000423"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR3953536.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(10813..11787)
                     /locus_tag="ON550_000424"
     CDS             complement(10813..11787)
                     /locus_tag="ON550_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR3953537.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(11768..13651)
                     /locus_tag="ON550_000425"
     CDS             complement(11768..13651)
                     /locus_tag="ON550_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR3953538.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13669..15894)
                     /locus_tag="ON550_000426"
     CDS             complement(13669..15894)
                     /locus_tag="ON550_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3953539.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16008..17042
                     /locus_tag="ON550_000427"
     CDS             16008..17042
                     /locus_tag="ON550_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953540.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17109..18122)
                     /locus_tag="ON550_000428"
     CDS             complement(17109..18122)
                     /locus_tag="ON550_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3953541.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18200..18388)
                     /locus_tag="ON550_000429"
     CDS             complement(18200..18388)
                     /locus_tag="ON550_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR3953542.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(18828..18956)
                     /locus_tag="ON550_000430"
     CDS             complement(18828..18956)
                     /locus_tag="ON550_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3953543.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(18959..20332)
                     /gene="rlmD"
                     /locus_tag="ON550_000431"
     CDS             complement(18959..20332)
                     /gene="rlmD"
                     /locus_tag="ON550_000431"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3953544.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRAILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20443..21498)
                     /locus_tag="ON550_000432"
     CDS             complement(20443..21498)
                     /locus_tag="ON550_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3953545.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21508..22938)
                     /gene="gatB"
                     /locus_tag="ON550_000433"
     CDS             complement(21508..22938)
                     /gene="gatB"
                     /locus_tag="ON550_000433"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR3953546.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(22935..24407)
                     /gene="gatA"
                     /locus_tag="ON550_000434"
     CDS             complement(22935..24407)
                     /gene="gatA"
                     /locus_tag="ON550_000434"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR3953547.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24407..24712)
                     /gene="gatC"
                     /locus_tag="ON550_000435"
     CDS             complement(24407..24712)
                     /gene="gatC"
                     /locus_tag="ON550_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR3953548.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(24818..26854)
                     /gene="ligA"
                     /locus_tag="ON550_000436"
     CDS             complement(24818..26854)
                     /gene="ligA"
                     /locus_tag="ON550_000436"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR3953549.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(26871..29105)
                     /gene="pcrA"
                     /locus_tag="ON550_000437"
     CDS             complement(26871..29105)
                     /gene="pcrA"
                     /locus_tag="ON550_000437"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR3953550.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29260..30819
                     /locus_tag="ON550_000438"
     CDS             29260..30819
                     /locus_tag="ON550_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR3953551.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(30930..32861)
                     /locus_tag="ON550_000439"
     CDS             complement(30930..32861)
                     /locus_tag="ON550_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3953552.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(32879..33793)
                     /gene="pfkB"
                     /locus_tag="ON550_000440"
     CDS             complement(32879..33793)
                     /gene="pfkB"
                     /locus_tag="ON550_000440"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3953553.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKKDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(33790..34542)
                     /locus_tag="ON550_000441"
     CDS             complement(33790..34542)
                     /locus_tag="ON550_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3953554.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(34702..34911)
                     /locus_tag="ON550_000442"
     CDS             complement(34702..34911)
                     /locus_tag="ON550_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953555.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(34922..36250)
                     /locus_tag="ON550_000443"
     CDS             complement(34922..36250)
                     /locus_tag="ON550_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953556.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            36386..36541
                     /locus_tag="ON550_000444"
     CDS             36386..36541
                     /locus_tag="ON550_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953557.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(36615..37202)
                     /locus_tag="ON550_000445"
     CDS             complement(36615..37202)
                     /locus_tag="ON550_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR3953558.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(37285..38535)
                     /gene="clpX"
                     /locus_tag="ON550_000446"
     CDS             complement(37285..38535)
                     /gene="clpX"
                     /locus_tag="ON550_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR3953559.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(38782..40071)
                     /locus_tag="ON550_000447"
     CDS             complement(38782..40071)
                     /locus_tag="ON550_000447"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR3953560.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(40225..41157)
                     /locus_tag="ON550_000448"
     CDS             complement(40225..41157)
                     /locus_tag="ON550_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953561.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(41314..41973)
                     /locus_tag="ON550_000449"
     CDS             complement(41314..41973)
                     /locus_tag="ON550_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3953562.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(41976..43001)
                     /gene="sppA"
                     /locus_tag="ON550_000450"
     CDS             complement(41976..43001)
                     /gene="sppA"
                     /locus_tag="ON550_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3953563.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(43014..43304)
                     /locus_tag="ON550_000451"
     CDS             complement(43014..43304)
                     /locus_tag="ON550_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953564.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            43713..45518
                     /gene="glmS"
                     /locus_tag="ON550_000452"
     CDS             43713..45518
                     /gene="glmS"
                     /locus_tag="ON550_000452"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR3953565.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(45590..46945)
                     /locus_tag="ON550_000453"
     CDS             complement(45590..46945)
                     /locus_tag="ON550_000453"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR3953566.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(46969..48138)
                     /locus_tag="ON550_000454"
     CDS             complement(46969..48138)
                     /locus_tag="ON550_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953567.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(48135..49022)
                     /locus_tag="ON550_000455"
     CDS             complement(48135..49022)
                     /locus_tag="ON550_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR3953568.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(49211..52903)
                     /gene="nifJ"
                     /locus_tag="ON550_000456"
     CDS             complement(49211..52903)
                     /gene="nifJ"
                     /locus_tag="ON550_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR3953569.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINERNTGYIQEIIEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            53102..53623
                     /locus_tag="ON550_000457"
     CDS             53102..53623
                     /locus_tag="ON550_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3953570.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(54322..54879)
                     /locus_tag="ON550_000458"
     CDS             complement(54322..54879)
                     /locus_tag="ON550_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953571.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(55058..55669)
                     /locus_tag="ON550_000459"
     CDS             complement(55058..55669)
                     /locus_tag="ON550_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953572.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(55782..56039)
                     /locus_tag="ON550_000460"
     CDS             complement(55782..56039)
                     /locus_tag="ON550_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953573.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(56636..56914)
                     /locus_tag="ON550_000461"
     CDS             complement(56636..56914)
                     /locus_tag="ON550_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953574.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(56948..57199)
                     /locus_tag="ON550_000462"
     CDS             complement(56948..57199)
                     /locus_tag="ON550_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953575.1"
                     /translation="MKEKPVNKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        15879 a        12469 c        10252 g        19074 t
ORIGIN      
        1 ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc
       61 tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat
      121 cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacca
      181 aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag
      241 aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa
      301 cacaccttgt tttttctgtt atataccgaa aaacggccat tcagttcaaa acaaggctct
      361 aaacaaaaac agtcgatcac ccattcattg atggatagcg acgattcgtt tactatttat
      421 cctgtttcta ccttttcgga agaatccggc tatgtaatca atactgattc caaagataca
      481 cttaggaagt ttgttcaaaa gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt
      541 tctgattctt ttgcaatcca agatatcaat gaaatattcc aagagatcag aaattgcacc
      601 aaaggacaac gtttctatca taagggaaag aaccttgctt atcagcatga acttcttccg
      661 ttattaacca accacgagca gcaagcaagt agttatttaa caaaaatcgt tcataaagac
      721 tacgctttag ctgtcaaaga acttgagaat ttcttcacga cgatattgga acagcctatt
      781 gatccagctt atttgaaaca tgaggcaatc aatcgtttct atgcattact caacgctttg
      841 gctgacgagc atgttgggaa tgaagaactt tctttgctca agatcgccct tccttcttta
      901 ttagccgatg ctgagtattt cgaagatttt tctattcttg tcaaagaaac acttgagaaa
      961 ctcacccact tttctgcaaa tgcaaaaaaa gacaatttcg atatcttgac tttgatctac
     1021 gagttcgtcc aggaaaatta tgagaaagaa atcaatttac aacttttatc cgaaaaatat
     1081 catttttcat atagctatct ctcagccatc tttactgaaa aatttggtat caacttttca
     1141 aaatatctaa aaaaagtccg tatcagtaaa gccaagctct ttttgacgga aacacggctt
     1201 actttgacgg aaatctgtga gaaagtcggc tataccgaac tgggttattt ctccagagtt
     1261 tttaaagaag agacaggaat gacaccttct caataccgta aaggaacttt tgtaaaatga
     1321 gaaaaaaaat caaagagcat gaactctttg ccaaacttct gttcattttg ttcatcggtc
     1381 tttttctgca agccatcgtc atttctttgt ttatttatca tcgatcaagg gatgcctata
     1441 tccagctgtt caatcaatcg aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa
     1501 gtttgaatga tacaatcgag aatacattag ccgcttttga ttctaatcca gctgtcaaaa
     1561 gctatttttc caatgatcca gcccaacata tggaacagct tcagcaacta agaacaatcc
     1621 aaaaaatgaa tgactctctt tcaaaaatcc atccaatgat cgattacgat gtattgatct
     1681 ttggagaaaa tggaagaact tttgtcggaa acgacatgct cactgccgta tctgccgatt
     1741 ctttttttca atctgcgatt gctcagagag tgaatgaacg tgctgctgat acacaaatgc
     1801 tttttgcaca ccacggattg actcttcgcg ataagaaagc acctagcgtc ttctttgtca
     1861 aaaaactgaa aaatacattg aatcacgttt acggatatgc tgttttatcg atctcatcca
     1921 agcaactagc gaatcttttt caatctgtag taaatcctga gatatcgaag atctccttca
     1981 tgaacgaaga acagacgatc atcgcatcaa atgaagaaaa tacaattggg agccgatcaa
     2041 tgattttcaa aaaactaaca gtaggtgaaa caacgatttc aaatggacag cagttgaccc
     2101 gtcttcctct gtaccgccag gattgtacat tactcagtca agtcgatctt cattctttat
     2161 ctaaccaaat gggcgtgatt tttccgatta ttttgtataa tatcgtgaca cttgcttttg
     2221 ctggctcatt ggtatttatg tatttggatc gacacacaaa atcgatctac cgcttgattg
     2281 attcgctaaa acggctagag gaagatggct taaaagctac tgttcctgta caaggaagcc
     2341 atgaagtcca attgctaggg aatacgatca atcaattgct aagtaatgct cagcatcatt
     2401 atgaacagac gatccaagac gaaaagaaga aacgttcgct tgaaatccaa actatgcaag
     2461 cacagataca gccccatttt atttataata cgctgactag cttgaaattt ttgatttggc
     2521 agaaaaaaac agatgaagca gttcaaggaa tcgacagttt cattacttta ctaagaagta
     2581 cgattgggaa aaaagaagaa gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt
     2641 acatcgatct tttgagtatg cgatatggcg aaagaatctc ggctaaaata atgattcctg
     2701 atgaactgca gtcattatct atgccgaata tgatccttca accaattatc gaaaacgcct
     2761 atcttcatgc ttttcacaat aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa
     2821 atcagcttct tttattcgaa gtgattgata acggcgatgg cttcgatact gaaaatacca
     2881 aacaaaaaaa tgatcatttt tcaggaatcg gcatcagcca tgtagatgag cggatacgcc
     2941 taatgtatgg cgatgcatat ggcttaacga tccaatcagt gattgggaca ggaacgactg
     3001 taacgatttg tctaccgctt cttcctaaaa atcaatcaga atatcaaaaa taaagaatag
     3061 ccaagcactg atttttttat tttaagttaa ataaatcaat cagtattctt gactttcatc
     3121 tacgcgtctc ttctactgtc agcactggat aacagcccaa tatttgactt tctcctccga
     3181 gcatcttgat ttcttggagc gcattctcta ctaatgtcat tgggcggtcc aacaacaaat
     3241 cgatcacgaa aaaatattct cccaaactgg ttttcaatgg tcgggattca atcttactta
     3301 agttgatctc ccgccatccg aaagctgcca gcattttatg aagcatccct ggccgattag
     3361 caggtagtgt caagatgaca ctcattttta ctggagctgg ctgctggctg gtcatctttc
     3421 gatcacctat gatccaaaaa cgtgtctgat tcaactcatt atcttggata ttctcagcta
     3481 agatctccaa tccaacatgc tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt
     3541 tcggatgttc tgctacatac attgctgcag cagttgttga ttctgttgct actaaaggaa
     3601 cattaggata atgcgtttct aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa
     3661 ggatttttgt aatctttgtt gctggattgc ctaaaagctg ctgcttgata ggaagaacga
     3721 tttcactttt aacttcgact tctggatgtt tggaaagaag atcgattgtg tgatgaacag
     3781 atccttccaa tgaattctct acaggaacga cagccagatc gacttgtttg cgaaacaaag
     3841 cgcgcaaaca agcagggatg gaagcatacg cagtcaattg ttctgtagga aatagctgac
     3901 aagccgcctg ataagtgaat gaactttctg gccctagata gctgacgttc atatgctttt
     3961 gatcctttct ataatggtat cgatgatctt ttctggtgtt tgattggtcg tgttgattcg
     4021 atgtgtcgca ctttcttcat accaagaaag tcgttgtgcc aataatgctt ttatttcttc
     4081 tcttgttttt ccaagagcta aaggtcgaat agacgtttca tcttgttgga tacgatcaac
     4141 tagacagtcc gcttctgcct ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg
     4201 attttctgca ctttggacaa tccctccacc agtcgcgata attttatctt catttgacag
     4261 ctttcttaaa atatctgact ccacttttcg aaagcttttt tcaccgtaat agtgaaaata
     4321 ttcggcaatc gacaattgga tatgttcctc aatctttgta tctaaatcta catactcttt
     4381 ttgtaaacgt tgagctaacc ctttaccgat tgttgttttt ccagcgccca tgaaaccaat
     4441 cagcaaaatg ctagccatga gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa
     4501 atgccggata agagattgaa acagcttcag ctccttctag ttcgcaaggt ccatcagcta
     4561 tcaaagcagc tacttgcagc atcataccga ttcgatgatc gcctctgctg gacacttttg
     4621 cgccatgcag aggtgtcggc ccatggatga tcagaccgtc tgctgtcgct tttatctttg
     4681 ctcccatttt ctgtaattct tctgccaccg catcaattct gttggtttct tttactttta
     4741 attcttctgc gtctttgatg atcgtgattc cttctgcttg agtagccact aaagctagaa
     4801 tgggcaattc atcaatcaga cgtggaatga tttctccacc aatcgttgct ttcttcaaat
     4861 gactcgtctt gacacttaaa tcagccgatt ggttctgttc attgtgattc gtctgggtaa
     4921 tcgctgcgcc catctcctcc agcacatcta agataccggt tcttgttgga ttgaccccga
     4981 cattttttaa taacagttgg ctttccggaa caagaagccc tgctgctaga aagaaagcag
     5041 ccgatgaaat atcgcctgga acagttatct gttgaccagt taatttttgt ggtcctgtta
     5101 ccgtgattgt tttaccttct actgagatcc ttccgccaaa ttgacgaatc atttcttccg
     5161 tatggttacg ggaacgttct ttttcaacga ttacggatgt gccttctgct tgtaatgccg
     5221 caaacagcaa agcagatttg acttgcgcac tggctacagg catattgtat cggataggtg
     5281 ataaagagtt ggttcctgtc acagtgattg gtagatactg ctcattctct tttccttgca
     5341 agtcagcgcc catctcgcgt aaaggttcca tcactcgccc cattggacgt ttagacaacg
     5401 aagcatcccc gaacagagta gtcataaaag gctgtccagc caaaataccc atcaataaac
     5461 gtatggtcgt accggagttt cccatgtcga ttgattcggt tggttggaca aatgagtggc
     5521 taccgcgacc atgaatcttg atcatctctt cctcttcttc gatttcaacg cctaattgct
     5581 ggaaggcatg taaagtactc agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg
     5641 ttccttccgc aattgcccca aacatgatgc tgcgatgaga aattgatttg tctgctggta
     5701 tcctaacagt tcctcttaat ccatgtattt gttgtaataa ttgcatttat ccagcctcct
     5761 atttgatcca gcaatgataa ctggtattag acataatgat tctttttgct tgtaacaaat
     5821 cctgttggtt tttaaaactg atttgcagta ttcctaaaat atcttccctg gtctcttgga
     5881 tcttcaaatt aatcaaagaa actcccgctt ttccgatcag tccagttact tctgcaataa
     5941 cgccaggttc atctggcaca tcgataaata aatcaaagaa ggcaggaatc gcgccttgct
     6001 tatggatagg caactggtct cgactttctt ttgcccgatt gaaaaaagaa aagatttccg
     6061 attggttctc agtcatgatc cattctgcta tttgtgccat ttcttgctgc caatccgcca
     6121 atcgttccaa taatatctgc ttattgctca acaaaatatc tgtccacata acaggatccg
     6181 aggaagcgat cctagtgata tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat
     6241 actcttgatt gaattgttct gcctgattga ctaagccagc tgccagaata tgtggaaaat
     6301 gacttagcat tcctgttata cggtcatgtt ctcttgctgt aagagttacg tattttgctt
     6361 tggttccgca aaatagttct tgcagttttt caacatcttt tttcgtcttt tccaaaggtg
     6421 taaagatgta ataggcattt tcaaatagtt tatcatctgc tgcctgtata cctgatttat
     6481 gagagccagc cattggatgt ccaccgacaa accgaagatt tttctgctca gcggatgtca
     6541 cgacttgctg cttcgtactg ctgacatccg taatgatgac gtgatcagac aagttgattc
     6601 tttcaaggac agcaagataa gctaaggttg tacggacggg aacggctaat aagataacgt
     6661 ctgcttgttc tgcacctgtt tcaaaatcat ttgcaacgta atcaacgatc tgctgttcta
     6721 ttgcttgctc acgggttttt gactgactat cccaaccaac tactatcagt tctggattgg
     6781 tctgacgaat acaaagtgcc atagaagcac caatcagtcc aagccccacg ataaaaacat
     6841 gttgtgccat cttatttgcc ccctttttaa taatgtttca agtagtcacg gtactccttc
     6901 actgcaactt tcaactcaac aagcgaatca cttgggaatt ttgacaatag ctctgtcgct
     6961 accacagtgg ctacaacatt ttccgcaacg acgctagctg caggaactgc tgtactatcc
     7021 gaacgttcta cacttgcttt gtaaggttct tttgtatcga tatcaacact ttgaagtggc
     7081 ttgtacaatg tagggatggg tttcatgacc ccgcgaatga caagtggttc gccattggtc
     7141 attccgcctt cgaagccgcc tagattattg gtacgtcgga aatagcctct ttcttcttcc
     7201 cataggatct catccatcac ttctgagcct ggtaatgaac cagaagtgaa tccgacccca
     7261 aattctacgc ctttaaaagc attgatacta accacagctt gtgcgatttt ggcatctagc
     7321 ttcgtgtccc attgtacgta gcttcctaat ccagctggaa cacctccaac gagcacttct
     7381 acaattcctc cgatcgtatc tccatttttc tttgtctgat cgatcaaatc ttttattctt
     7441 tgctcaactg ctggatcaac aactcgtact tctgacgctt cagaaatttc tttgatttct
     7501 tgaatcgaga tatttccagg tatttctgct tggattccac caagacaaat cacgtgtcct
     7561 gctacgatga tgtcaagttc ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc
     7621 atggtcgtct ctcttgcaga ggaccgttca agcacattcc gaagatctcg atgatggtat
     7681 ttcataccgc ctaccaaatc cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat
     7741 tggtcttttt caggcacttc tgctacagac atgacagttg tccaattttt ccaatcttta
     7801 ttttctacca ctaaagtgac aggtgaacca attgttttgc cgtgacggat gccagacgtg
     7861 atccgaacac gatctttttc gatcttcatc cttcctcctc tgccgtaacc agcttgtctg
     7921 cgagcgagtt cgtgattgat ttcttcttct gacagaggta atcctgctgg tagtccttca
     7981 ataatcgctg ttaattctgg tccatgggat tctcccgcag tcatataacg catcttacag
     8041 tcctcctaag taatctttga atgtctctgt gggaacagga acgatttttg cttgcccaat
     8101 cttttctagc aagatgatat tcaattggct tcctcttgct ttcttatcat gggttatcgc
     8161 gtggaataat tcatctggat gacttagatc atacgtaatc ggcagatgga atttttgaag
     8221 tatcctttct aaagcagctg tcgatccagt tggagacaag ccgatttttt ctgcatgaga
     8281 agtgatggct accattccaa tagctactcc ttctccatga ctgactaccc catatcctgc
     8341 tgtattttca attgcatggc caattgtatg accaaaattt aaaataagtc gatttccttg
     8401 gtcaaagaca tcttcttcca ctacttttcg tttgatttcc aacgtagcag taatgattgg
     8461 aacagcatgt tccagaagat ttttttcgtc tttcagctcg ttcagctgtt tccaaagcgt
     8521 tgcatctgct atagctgctg atttgatgat ttcagcgatt ccttctcgta aacgacgtac
     8581 cggcaaagtt tttagaacct caggatcaat caatacacca gctggctggc taaatgttcc
     8641 gacaaggttt ttcgctttgt ttgtattgac tgctgtcttc ccgccaatac tagaatctac
     8701 ttgtgcaagt agcgtagtcg gaatttgaac gaaatggatc ccacgcatga acgtcgaggc
     8761 cacgaatgcg gctagatcac ccaccattcc tccaccaagt gcaagtatcc catcgctgcg
     8821 ggtaaaatca ttatctgcta aaaaatcgta taactcagct gcttgttcta aagatttact
     8881 ggtctcaccc gccggtacga ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa
     8941 catgattttt tctgcatata gtggagcaac gtttgtatca gtgatcacag ctactttttg
     9001 aggtctccaa atcgtttgaa gccatgatcc agcctgttct agtgcgcctt tttcaatatg
     9061 aatatcgtat ttttggttat caacatgtac agtaagcatt catttcactt ctttctttta
     9121 aaaagctgga attaatcgag tgtctgaatc ttttccattg cttctttcat gatcgcaact
     9181 tctttcatca tgcgtgagta agtcttttca ttcaacgatt ggggaccgtc tgaccaagca
     9241 tttgcaggat caggatggat ctctacgatc atgccatctg ctccgcttgc cacacccgca
     9301 cgagccattg gtggtacaag ttcccaaatt cctgtaccat gacttggatc aacaatcaca
     9361 gggaaatgag ttaatttttt catcaatggg acagcactta aatcaaacgt attacgtgtc
     9421 gctgtttcgt aagtccgaat cccacgttcg ataaaaatga caggagattt gtcttcgact
     9481 gcaatgtatt ctgcagcatt aagccattca ttgatcgtac cggaaattcc acgtttcaat
     9541 ccaacaggtt tacctgtttt accgacagca gatagcagtt taaaattttg catattacgt
     9601 gcgccaatct gtaggatatc gctgtactca gccaccatgt cgatatgccc ttcatccatc
     9661 acttcagtaa tcactttcat tccaaattca tcagccgctt gacggatgta tttcaatcct
     9721 tcttcttcta aaccttggaa agcatacgga gaagtacgtg gtttgaaggc accacctcgc
     9781 agaatttttg cgcccccggc tttggccatt cgcgcacatt ctctgatttg gtctaatcct
     9841 tcgatagaac aaggaccagc catcgtcacg aaactgccgt caccaatttt tactccgtct
     9901 acatccacga ctgtattgcc aggatggaat tctcgagaag tcaatttgta tgtgtttaaa
     9961 atacgcacag cattttctac tccttcatag cgtgtaaatg ctccttcttg taaacttcgc
    10021 gtatcacctt ttagtccaat cactactcgt tctgttcctt gatcgattga gactttgaat
    10081 ccttcttttt cgatatgatc gacgactttt tttatttgtt cttccgttgc ttcttttttc
    10141 ataattacta tcattgtttt tcccgctttc ttttgtttac ttatctatct tggctgattt
    10201 tctctacttt ttcaaggtgg ttttacatta attcctacta aatgaccgtg tcctgttgtt
    10261 ctcattttcg ctccttcttt cctacttatg acccagataa tcaacaaaaa aagatgtccc
    10321 tgtaggaaat tgcctctaca gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat
    10381 ataaaatagc cactatagat gaatttctct acgtggctgt aagtcgtgtt taaaaatctt
    10441 tagccatcat agatacaaac ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta
    10501 tctaaaatag ctaaagtaat acgtattcag ttttgtaagt tttctagatt tcataaaaaa
    10561 tcgctccaaa ctgatgtaag aattgtacct agtatagaca taaaaaaatc gactgtcaat
    10621 catttttatg aaactttttt taattttctg atagttaact ttgtttttca gaaaaataac
    10681 aattggtttt attgaagaaa atagtcatga acaaaagtat ctgagaactt ttttactctc
    10741 ttgttcatga catatagaat aaagcagctt tgtcttgtaa actccgtttt tcaaataata
    10801 atctaaagat tgctactgaa taataaagcc gatttcatgg tcctcttcgc tgctgattcg
    10861 gtaaggtgac tcaacatccg ctccccagct attgataccc ccgactcctc ttacttttgc
    10921 aaaaatcgta agaaccgttc gtctttctgg cggtagttct tctatatgtg ttgcctgttc
    10981 cagttcttct gctttgaatg gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa
    11041 tcgcaacgaa aaatcttctt gatctttatc cgtattatcc aacgtttgat ttcttgttag
    11101 ttctacccat ttcgtttcca tatgcatgcc gcattcttgg ggaacaaggt atggggaaac
    11161 ggggagtcct gccactttat aaataccttc gacaccacca gccatccgat ccggatatgt
    11221 ttcccctgat aggccttcat actcaaaaaa ttttgctttc gttggcataa tgaaccttaa
    11281 acccaatgcg ggtaattccg gtaatccttt ttgtccataa taatgcatat tgacaagcat
    11341 tttaccctct ccagcgattt ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat
    11401 ttccagtgta aaaatgattt caaccgtttc agcgtattca ttacttgaat attgattatt
    11461 tactggagca atcggcaaat cgatcttttt attgtccacc agtacttcta tttctttgac
    11521 ttttggatac aatccggctc ctaaccacat accggattta aatccaaatc cgctacctct
    11581 gtcattatcg gtcgttgctc gccaaaaaac tggaagtgtt tcccggtata gccattcttt
    11641 tccgttttta ttgagcgatt ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt
    11701 ccccgatttg acacctaatg tacagtcgcc gaagacgatt gcaacttttt ccgtatccat
    11761 atgactttta tttactgtat ttttcataat aatatcttac ctcctgcatg gcgggttttt
    11821 cttgtccatc tgcaaaaacg atcccattcc ctgagaattc atagtcactt ggtttatcgt
    11881 caaagtcacc gccatagcgc atcacttctc tgcctgtgat tggatcagtc acttggatcg
    11941 cctgatcttt aaaatcccat atatagcctc cttggtacat ttcataacga tcaagcaacg
    12001 tcatgtaaga atccattcca cctaacgaat tccccatgtc atgcatatat tcacataaga
    12061 tgaacggttt ttccgcttta ttttcaaggt agtcaatgat ctcttctggt ggcgcataca
    12121 tgcagctttt tacatctgaa atcgaatctt cgaatgctgg atttggaaaa ttcccttcat
    12181 aatggacaag tcgatcgggg tctacttttt tgaagtaatc gttcatttgt cgaatgtttt
    12241 cttccgcata agattcattt ccaagagacc aaaaaagaat acttggatgg ttttttaatg
    12301 tttcgaaatt cgtttttgct cgatcaagaa cagccgcctg ccactgaggt actgcacctg
    12361 gtacgttgta agaaggctca aatgcgccca ttttttgcca tgagccatgg gattcgaggt
    12421 tcgtttccgc catcacatgg attcctgctt ggtcgcataa ataataccaa gtgatctggt
    12481 tggggtaatg agatgtccgt acgccgttga tctttgcatt ttttatgata gccaaatctt
    12541 tttccatatc ttctatagtg atggcgcgtc cttttttcgg atgccactca tgtcgattta
    12601 ccccgttgat aatcatccgt tttccattga agtagatgat tttatctttg atttcgattt
    12661 tacgaaaacc gaaggaatag tcaatatact ccgttactct tttttcttcg tctatcgtct
    12721 ccaattcaag cttatataaa ttgggctgaa aatgccccca cagtgccaca ttttcaaatc
    12781 catcgatgcg aatcgtcatt tgctctttta acggatattg ctgttgatag atttccttcc
    12841 catcaggatc tagtatcgct acattcagct gactgttttt ggctacttgc tcaaaagaca
    12901 aatctacttc aagtttgcca ttttgacatt tgtcatctaa gcgaggtttg atccaaagat
    12961 cttctaaatg gttttgtcct cgagcaacta agaagacatc tctgaagatc ccaaaaaatc
    13021 ggaaaaagtc ctgatcttcc aagaatgcag ctgtacttct tttgtaaacc tccacagcta
    13081 gaatattttc ttcttccaaa taaggtgtta aatcaaattc agacggagtg aacgaatctt
    13141 cggcataccc gataaaatga ccattcaacc aaataaacat tgcttgttct acaccagcaa
    13201 aatgaatcga gatttgtttt ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg
    13261 aaccaaccgg attgtcttta gcagctgaaa agatcccttc cgcctctttc tcagggcttg
    13321 tcgtgaaagg gggacggcga aatacatgtc cctcccacgg atacatcgta ttgatgtaat
    13381 ggatcttgtc ataatttgct aattcaatat gactaggaac agtgatttca tcaaaagaac
    13441 aggcatcaaa atctttttta taaaatgttt ttggtcgtac cgaaggattt ggcgaataac
    13501 aaaaacgcca tgatccattt aaagactgtt taagggagga tgtggtttct acttcttccc
    13561 ttgttgcata acaaacatga tcgctatgtg catttgattg gttcactcta aaaacttgtg
    13621 gatcatctaa ccattttata tttgctttca tacttttttc cttctctctc ataacttttt
    13681 gaaatgaagc agttctgttt ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt
    13741 catcaattcg gcccccgaaa atatccgctt cgaggtctgt tcctgataaa gtgcctcatc
    13801 atctaacccc atcagatata cttcatggaa cgctggttga gctgaagcta gtatcctccc
    13861 taaaaacaca atagcttcat cttgtttagg agacacaaac atccatgcgg caatattttc
    13921 ttcaaaagga ctcgccaaac gataataatg gccatactgg atcaccggac gaatcgtttg
    13981 ataggatata atttggtctt ttacttgctt tttttcttca ggagacagct ttgtcaaatc
    14041 cagttcataa ccaaaaacag cgctcatagc tgtttctcct cttgttttca acgaagtaga
    14101 tctgtcggtc tgatgatttg gtacagctga tacatgagct gtgatggacg aaatcggata
    14161 agcaaggctt gttccatatt ggattttcat acgctccact gcatccgtgt tatcacttgt
    14221 ccatgactgc ggcatataat aaataaaccc ggcatcaaac cgtccacctc caccagaaca
    14281 gccttcccat aagatttccg gataatcagt tgtcaaatgt tcaagtagtt cataaagccc
    14341 gagcatataa cgatgcgcta cttctccttg tctctgtggg tcaagggtaa tcgaatagac
    14401 atctgaaagc gaacggttca tatcccattt gatgtagtca agaggaatcg tatctaaaat
    14461 ctttctcatt tgtttctcaa tcgtctgccg aacatctaac cttgtaaaat ccagtacatg
    14521 ttgtgatctg gaagcagaag gcattctacc tggctcctgc ataagaaaat caggatgtgt
    14581 tcgatacagt tcagaatcaa ccgagatcat ttcaggttca aaccataaac caaatttcaa
    14641 tcctttgcta tgagcataat ctgctatttc acgtaatcca tttgtcaact tgccttcata
    14701 ctcgaaccag tctcctaatg aagaattgtc cgcatctctt tttccaaacc agccatcatc
    14761 taatacgaac atttcgatgc ctagctcctt tgcttcatca atgatcgcct tgattttttc
    14821 actattgaag tcaaagtaag tggcttccca gttattgacc agaatcggcc gttcagcaaa
    14881 ttgatgacga cctcttgcga ttctttcacg tagcaactca tggtggatct gactcatttt
    14941 gttcaatccc tgacttgagt aagaaaggat catttcaggt gtttgaaaag actggcctgc
    15001 attcaattcc cacgaaaaac gatggctgtt gatcccaccg acgactctca attgattgat
    15061 ctgatctttt tcaagttcga aagaatggtt gccggaataa acaaattgaa gtccgatcgc
    15121 ttctccagta ttttctgtcg tatgatgatg aactaaagca ataaagttat tcatatggtg
    15181 actgctcgtt cctcgacggc tttcaaatac ttttgttcca gataaaacag attgccgtga
    15241 aatctgtctt tcattgacat gtgctcctgg caaagcaatg acttcatcaa atcgcctagt
    15301 atgggcaaag tctagctgga aactagccgc tttttctagt ttgattgttt ctccggtttc
    15361 atttcttatc tttacagatc gtgtgatcac tgctctatca gtaaatatgg tatagaacaa
    15421 atcgaaataa atcgctgttt ctctatcctt aagtgaaatc accaacgttt gtgcctctga
    15481 cttgtccatc acataagttt gaggcaagcc ttcgatctct ggcttcccat ctgtaatata
    15541 atggctgtca tatctcagat ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc
    15601 gggcgttcga aagtccatgc tgccatgaga agagtattct tgcggtagtg tatctttcga
    15661 cagagaccgg tcaggattta agggaacatt tcctgaaaat ccacgatctc ttcggggata
    15721 atttttattg tctttgtact gcttgactgg tttgccaaaa taaatatgcg ccaatacatt
    15781 gctttcttct atctttatca gataagaaag aaatgtattt cgtaaatgga agactcggct
    15841 cttttcatca aataagatga aatttttatc tgtttttcta tgcgtaatat ccatcatttc
    15901 actcccaaca aaacggaaac cgtttacttt ttttataatt cctgtatact gtttactaaa
    15961 caattttgtc aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta
    16021 gagatgtcgc acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg
    16081 atcagacgat ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt
    16141 tggattatga catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga
    16201 tctctacgat cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag
    16261 ggatggaaga agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg
    16321 ataatcctaa acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg
    16381 ttacgaaaga atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata
    16441 accctgacat ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa
    16501 ccgtactgga attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata
    16561 aagtcgatat ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg
    16621 cctatcagca ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg
    16681 gctggacaga aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc
    16741 ttcctactgc cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga
    16801 aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga
    16861 caatcgcaaa cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc
    16921 aagcagctgt tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt
    16981 tgacttatcc tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt
    17041 gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc
    17101 acaacctctt acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat
    17161 tcttttagcg tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa
    17221 aacaaggatt gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc
    17281 tcctggaatg agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca
    17341 tggtcagcca cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc
    17401 aacgaccacg caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt
    17461 gctgttagct tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt
    17521 ggaagtagct gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca
    17581 tattgcaatg cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct
    17641 agcaatttgt cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga
    17701 acttgattag ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg
    17761 ttggcgtctg aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg
    17821 aatcctccac catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag
    17881 tagatgtcat aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc
    17941 acgttttttt ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac
    18001 atatggttga tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca
    18061 aacgcgatcc caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc
    18121 ataaggctcc ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta
    18181 tcaagctctc ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca
    18241 aaagaaattt tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc
    18301 cacattgagg acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc
    18361 ggtcaatcac ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag
    18421 acaactaatt tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta
    18481 tcgtattcta aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc
    18541 gtcatccccc ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt
    18601 agtgttgaat catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa
    18661 ttgaaataac gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc
    18721 acaactcaac agtccttatt ggcaaatcgc caaaataatg tagactaact aaaaagacta
    18781 agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta tttattaaag
    18841 attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat ttgtttggat
    18901 cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg gtccattttt
    18961 attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg aaaagatcta
    19021 caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca tctcttgcaa
    19081 aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg attgctgatt
    19141 caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca gatggtttga
    19201 tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca taatgaacat
    19261 tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct ggaataactt
    19321 ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta cctattcctg
    19381 aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg gctgtttggt
    19441 acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag attcgatagg
    19501 tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt tctttgtctc
    19561 ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc acttctggca
    19621 attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct ttacgagtaa
    19681 ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg tgacgaagct
    19741 gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag atagctctga
    19801 ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg atagggacta
    19861 gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata cggcgaacag
    19921 ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt ggaagtacag
    19981 gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt tgtttgaaga
    20041 caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga agtaatgcat
    20101 tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact tttccatatc
    20161 caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg agtgcatttt
    20221 cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa ctcaagtcga
    20281 cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc atattttgct
    20341 cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa ttacacaact
    20401 aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct tttgattagg
    20461 tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac gttcgacttc
    20521 ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg tgattgcttc
    20581 atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat tttggaaatg
    20641 catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc gattgatatc
    20701 atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg gatcatcgac
    20761 atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga tatttgctgt
    20821 cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg cgatctgttc
    20881 gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc cgatatattc
    20941 cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca tctctgctcc
    21001 tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa cttcataagt
    21061 caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat cagaaaggcc
    21121 gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt taatattatc
    21181 tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga tgatggccat
    21241 tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg tgccatcgcc
    21301 accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt cattcttggc
    21361 tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt cttctaatgc
    21421 ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg ggttataaat
    21481 tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt taagtagctt
    21541 attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac cgactaggaa
    21601 gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt tgttatccaa
    21661 aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc aaccattttt
    21721 ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga cttttttggc
    21781 gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta agttctcagg
    21841 tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag aaacttcacc
    21901 catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag cttcaaaaaa
    21961 atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa gtccaaactc
    22021 acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt ttactttttg
    22081 aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt aacgataatc
    22141 actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt caaaacggcg
    22201 tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt tttcttcaaa
    22261 agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg ctttcgtacc
    22321 gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca tagagccttc
    22381 ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg cttctagata
    22441 agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga tcaaaggcgt
    22501 gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt tttttccagc
    22561 atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt ctacttcgat
    22621 ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat atgcttttgg
    22681 attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga tttcacagtt
    22741 taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta ctggcagcac
    22801 gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac caaaatgagc
    22861 tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt ctagtccaat
    22921 gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg tttatggaat
    22981 tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc aaaataatta
    23041 ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac agacatacct
    23101 ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat tgtgattgga
    23161 tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc gatgatcaag
    23221 tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac ttgtcctgct
    23281 tttttgaagt aagcgtcata ataacccgcg cttaaggaga atgtacctaa catgatacgg
    23341 cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc atctaatgat
    23401 ttcacatttt cagagcgata accgtagcgg ataccatcga atcgttgcaa attggaactt
    23461 gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg aggcaagctg
    23521 acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa tacagcttct
    23581 tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat cttcattcct
    23641 ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga agaagtaccg
    23701 tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt caccgtacgg
    23761 gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa acgtgagaca
    23821 cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg gcggatactt
    23881 cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc tgctgcagaa
    23941 ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt tttcttgaaa
    24001 taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt accaacaggg
    24061 atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg ttcaaaattg
    24121 taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt atccttgata
    24181 ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc taaagctttt
    24241 gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cctcgacctc aggctctgtt
    24301 tctttgattc gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc tttcgataca
    24361 agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat gcgccagcct
    24421 ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc ggcacatttt
    24481 tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc acgtttactg
    24541 tttcaaccac attcgacgta aatggaacac cttccgtatc tacttcttcc agcatttcaa
    24601 ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca tctgcgaacg
    24661 caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc atactacccc
    24721 ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg ccgtgtctga
    24781 aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact gagcgacttt
    24841 tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat tcttgtgctt
    24901 tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt ttcgatacgc
    24961 taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca cgagtaaatc
    25021 gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg acttcttgca
    25081 attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt aattcattca
    25141 tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca atgatatcac
    25201 cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt tctagatcac
    25261 cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt cctaacccga
    25321 aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa atattatttg
    25381 ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat ttgtaaagat
    25441 cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc ggccccaacc
    25501 catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc atttgagcag
    25561 ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca agttcacttc
    25621 cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt cgtttatcta
    25681 acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga actgtatctt
    25741 tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca cggcttactg
    25801 tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc cctgttcgac
    25861 cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga aacttgtaag
    25921 ctgctgccca tctcggtgct ttgatagtaa aaccaagttg atcttgtagc gagaattcat
    25981 tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg tcatgatatt
    26041 cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc cgattcgtgt
    26101 ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct tgcataggac
    26161 cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg attttagaat
    26221 ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata tcttttccct
    26281 ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa cattcgcctc
    26341 ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca gtttttaggt
    26401 tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc acgaaatttc
    26461 catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag caatagtcaa
    26521 ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct tctttgctga
    26581 aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa ccttctaata
    26641 cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga tattgttcct
    26701 cgattgctac taattctgcg tattctttat cataaacata gtcttccact gttggtttat
    26761 cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct tctgctcgtt
    26821 tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta ctaagctttt
    26881 tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa agccacatct
    26941 agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc ccattttttg
    27001 tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc acttttagag
    27061 ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc gaatacaggc
    27121 gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc gatttcttct
    27181 acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga gaaagcattt
    27241 gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg gcgttcttcc
    27301 tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc ttctagacca
    27361 atcaggaata caactggaaa ttctaatcct ttggctgcat gcaaagtcat caatgtcact
    27421 tgggaagcat cttcttctaa attgtcaatg tcggaaacaa gtgccagatc atttaagaag
    27481 accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc gaattgtttg
    27541 tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc ttgtgcttct
    27601 aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac ctctttcgtc
    27661 aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat ctctccaaat
    27721 gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt ttgcgctgct
    27781 tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat agatcctggt
    27841 ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc ttgaggattt
    27901 accagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta gaacttgtgc
    27961 cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat tacacgagat
    28021 tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt gcttcgaatc
    28081 tcttcttgca tccggtctac gataaaacgg gtttcgtctc gttcattatc tgcacggtag
    28141 taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt tcgattgcta
    28201 ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg atagttttgt
    28261 tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat gttctgcata
    28321 tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca caggttccta
    28381 aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc ttggtactca
    28441 tctacatgaa tataatggaa tttgttttga taatacgtga gtgaatcagg atgttcttca
    28501 aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt atattgaagc
    28561 tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact gccttgcatt
    28621 tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt tcctaatata
    28681 cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg tttcatcaag
    28741 gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat aaaatcaaca
    28801 tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac atcttcgcct
    28861 ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt attggtaaaa
    28921 gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata ggcaatgcgg
    28981 tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa tggtccgtca
    29041 gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc agctttggat
    29101 gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt cttggctttc
    29161 attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct tgtcaattcg
    29221 ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa aggaaaaatc
    29281 aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt tggactgctg
    29341 acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga tttagttcga
    29401 agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc tatttgctgc
    29461 atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat caagggaagc
    29521 gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg gttctctgtt
    29581 ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt tctaggtaga
    29641 gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca attgtttcgg
    29701 agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg acttgcagca
    29761 gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca tcactccttt
    29821 tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt cgtctctctt
    29881 tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt gccgttgaaa
    29941 tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt tatttatcag
    30001 aatgtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc ttattttcac
    30061 gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag tactctaggc
    30121 ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat tatgattatc
    30181 ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggggg tcttccaggg
    30241 gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta tggaaacatt
    30301 atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat tgccatggct
    30361 ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt gacagaaatg
    30421 gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc ttatatcgtc
    30481 aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct acttggaaat
    30541 actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt aacggcagag
    30601 agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat gctgctaatc
    30661 tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat gaatgtagga
    30721 gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca ggaaatcgag
    30781 gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa aaactatttt
    30841 acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa agaagaggct
    30901 gggaaaaccc agcctcttct tttttatttc tatgcttctg ctactgcagg agcttttctc
    30961 aataaaccat aaatcacacc agatacgact gctccgatag caataaacac caaatacatg
    31021 accgggtgac tcagtaagaa tacaacgaaa atcccgccgt gaggagccag taatttgatt
    31081 ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact tggaatggca
    31141 cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga tagtcccatc
    31201 acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt aaacaaacgt
    31261 gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga agccatcacg
    31321 acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac ataagcggct
    31381 ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc accaagtaaa
    31441 gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt gattgctttc
    31501 atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa cactggataa
    31561 aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa tttacgtaaa
    31621 aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa tcctgcaccg
    31681 cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg gtcaccgata
    31741 cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc ttgtccgatt
    31801 tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg atcatgtggc
    31861 acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc accaataacg
    31921 aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg tccgatagta
    31981 ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa tactggtgca
    32041 gtaccactaa gggctagatt gatcaactct tctggtttac ggataccatc actgacagga
    32101 cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc tgctgcaacg
    32161 attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat tccttctgaa
    32221 ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt caaagcatct
    32281 tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac aacgaatttt
    32341 ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt ttcttctgct
    32401 tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt atttgcttct
    32461 tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc taatgcttgc
    32521 aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata agttggctgt
    32581 ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac tgttgcttct
    32641 ttaactgcac tgtttttcgc gtgaggcatg gcgattccgt caccaagacc tgtagaagtt
    32701 tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat ccggccggca
    32761 tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc ttgcaaatcc
    32821 atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct ccttgaaact
    32881 aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg cgagctgcca
    32941 agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag gcttcgattg
    33001 gatctttcgt acggctgtaa gccccaataa agccagcaat cattgaatcc cctgcaccta
    33061 ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt tccgtgaaga
    33121 gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca gctagtaatt
    33181 ttttcccata cggaatgatt tcttctacag attggaaggt tgtgttgtaa agctcagcta
    33241 attcgtgatt gtttggtttg atcaacaacg gtttcttaac aagtgcattt tttaggtcat
    33301 cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca atcagttctt
    33361 gataaaatcc ttttcgcagg ctagctggcg cgcttcctga caatacgacg atatctcctt
    33421 gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa atagatgggc
    33481 ctaagccatt gatctcagtt tcagtgtctg attttagttt gatattgatt cttgtatctt
    33541 tttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc gaaataaatt
    33601 cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc cgtttcaaaa
    33661 tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc attcggttca
    33721 aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga ttaagtgtta
    33781 ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt cattaacgaa
    33841 ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa cagaggtgaa
    33901 ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg aatttttcat
    33961 ggctaaacgt ttaacagctg cctcttctgg atccggagtc gtgaatcctt gttcaagatg
    34021 ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt gttctatcgc
    34081 attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa taatggtagc
    34141 aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta ctgtgatctg
    34201 ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta aataaatgac
    34261 atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt cgtgaacgtt
    34321 tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt tcttagctcc
    34381 gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac gtcttatcgt
    34441 tgattcagaa gcattcaaaa ggtttacaag atcttgtgat ttcacgattt tctgctgttc
    34501 aagcaagtct aggatttttt gtctgcgttc ctctgtaagc attttcacga tcacctcaat
    34561 acaaataata gcatattttt tttctactag caatataaat cagtcaaaaa cgttcataaa
    34621 aattataaat cagtcaaaaa aaaaataaaa aaccagtcag aaagactggt tttttatttt
    34681 tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt ttttcatata
    34741 aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca tcccgccaaa
    34801 catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa agagacagac
    34861 ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca ttttcttcac
    34921 ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa aattgcacac
    34981 gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt ttctttattc
    35041 gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat tctgacaggg
    35101 attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt tcatctgtta
    35161 cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca ttatccaaag
    35221 caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg tcaacccaag
    35281 gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa tctccttgtt
    35341 ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca ggttgcattt
    35401 gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat tcaacgaatg
    35461 gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca agctggtccc
    35521 taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg tctcgataaa
    35581 gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt tggatgtcga
    35641 gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt tcccaacacg
    35701 aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg caatcgacta
    35761 ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc tttttctcaa
    35821 acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta taagctcgca
    35881 tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga atcaaaaatt
    35941 gcttttcttt gaaaacagag atagacaata cattttttat caataattga ttatcttcca
    36001 agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg tctcttaccg
    36061 atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct tgttgtgtga
    36121 aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt
    36181 cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat aatttatctt
    36241 cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa ccaatgataa
    36301 agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga tagactaagt
    36361 aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag ataatttaga
    36421 taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat
    36481 cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta
    36541 agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg actggtaagt
    36601 cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc ttcttctttt
    36661 ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa atccaacgct
    36721 tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc tgccttagtg
    36781 gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg gatatcttct
    36841 tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg gtctctagtc
    36901 gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga tacttttgca
    36961 tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata aaaattcaat
    37021 gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc aatcaatgtg
    37081 ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc tggcaattgt
    37141 gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg atggactttc
    37201 atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat
    37261 gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt tatagattac
    37321 tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt cgatgttttc
    37381 atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg aacgaagtcc
    37441 tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg cttcaggttc
    37501 gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga ctagcgcatt
    37561 tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta aagctgccat
    37621 aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat cttcaggaat
    37681 gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac caaatccaat
    37741 cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg caccacctac
    37801 gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat gtttacgtcc
    37861 gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg cttgttggac
    37921 accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg caattttatc
    37981 gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat cagcagattg
    38041 aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt ctgtcaaact
    38101 tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt gtgctaaaaa
    38161 tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca gttcaacatc
    38221 atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata cggcaacagc
    38281 taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt caagaatttc
    38341 tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt cttcatcaat
    38401 gatttcttta cataggtcga tacattcatt acagatgtag acccctggac cagctacgat
    38461 ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct cattgctact
    38521 tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag aaactatcaa
    38581 agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag tgtttaacaa
    38641 aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa ggaactgtga
    38701 caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta gcttcttcag
    38761 aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg tgatcaattc
    38821 gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca cgcgtttgat
    38881 ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat tgatttcttc
    38941 ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat ttgttcttgt
    39001 gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag aaccagttaa
    39061 ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga attcgtccat
    39121 tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc
    39181 aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc
    39241 tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt cgtcatcaac
    39301 atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt catggattgt
    39361 cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt ctgggaaagt
    39421 tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt caaaacctgg
    39481 aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc catcaaacgc
    39541 ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt cagctgcagc
    39601 atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac gtgcatccac
    39661 gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat attgtcctaa
    39721 ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac ctttttccat
    39781 gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt tgacagcagc
    39841 ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt cttcaccgta
    39901 catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta cgttcaagct
    39961 ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt
    40021 aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca tttaattccc
    40081 tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct ttaaatagta
    40141 cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt ttcataacat
    40201 ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca aaattttttc
    40261 tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg tttttcctgt
    40321 acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg tatcatagac
    40381 aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac taagcagcgc
    40441 atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg cctgctgctg
    40501 cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg tcaaatagaa
    40561 aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc ttgctaaaat
    40621 atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat gataaggaag
    40681 ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag gaagttcctc
    40741 cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata attcttctgc
    40801 aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg tacttgctat
    40861 caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat aaaattcagc
    40921 aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca atgcttcttt
    40981 tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa agctttcttt
    41041 caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt ttgctagtgc
    41101 ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg gcttcacgca
    41161 gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg
    41221 acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga cttttagaag
    41281 gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct ggttgaacgc
    41341 tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca gatgttgctt
    41401 tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt ttagaatgaa
    41461 ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt catcaaagct
    41521 gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga atttcgtcaa
    41581 taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac tcagataaga
    41641 atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac gcgtaatagc
    41701 tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa
    41761 atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat actttttcca
    41821 tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa aagagctcag
    41881 taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct tatctttttc
    41941 ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta ataatacatt
    42001 gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg actagtctca
    42061 gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact agcaaaccca
    42121 gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc ctttcgtata
    42181 gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc ttttgcttgg
    42241 acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga tttattccgt
    42301 ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc
    42361 gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt
    42421 ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt caatcctgac
    42481 atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt atctgcttga
    42541 gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata cattgggata
    42601 tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc gtagacaccg
    42661 ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc ttcaatcgcc
    42721 ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa acctgaagac
    42781 cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt actgtctccc
    42841 tcttccaaca catttggagc tagttcttca tccccatata accaactgtt caatccctca
    42901 atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat
    42961 cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc tttctaatac
    43021 aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg ctctgctttt
    43081 tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac cgtaaactca
    43141 cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc ccagccagta
    43201 tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt ggacaattgt
    43261 ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac tcctcctcta
    43321 tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa acccattatt
    43381 ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta ttttttattc
    43441 tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc cagataaaaa
    43501 acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa acagatgacg
    43561 aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc tacagaaaat
    43621 ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg aagcattcat
    43681 acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga atgattggat
    43741 taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac cgtggttatg
    43801 attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa gcacaaggca
    43861 gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata ggtattggtc
    43921 atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca catacttccc
    43981 aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa
    44041 ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt atcgctcatc
    44101 taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa gctttgcggg
    44161 tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat gttatttacg
    44221 tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat gtgattgcca
    44281 gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa gatgaagaga
    44341 tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac atcgtagaac
    44401 gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc tatccttttt
    44461 atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct caaaaatacg
    44521 taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg tctgatagcg
    44581 atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca ggtaaacaga
    44641 ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa tttggctata
    44701 acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt ggcgaaacag
    44761 cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca cttacgatta
    44821 caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta ttgcatgctg
    44881 gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct gtactaactt
    44941 tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta ttcgatatca
    45001 ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa aaagattatt
    45061 tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac attggacgtg
    45121 gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc agttatatcc
    45181 aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg atcgaagaag
    45241 gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact cgaggaaacc
    45301 tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag ggattagaaa
    45361 aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact ctaaccacag
    45421 tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc gacgttgaca
    45481 aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag gaccagtgtc
    45541 agacatctga cactggtcct tgttctttta ttttttatga aaatatctct taatccagtc
    45601 cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact ttctcatcag
    45661 taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga actaggattc
    45721 ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt gccggaacat
    45781 ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga tagattgtta
    45841 cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc agtaactgaa
    45901 ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg tgcccagact
    45961 gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa
    46021 cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat cctaagttac
    46081 tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc aagtatttag
    46141 cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc accgcaataa
    46201 tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca acgaccatct
    46261 cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac ccgtctggat
    46321 ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta acacttgttg
    46381 aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca ccgttgatcg
    46441 tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct acagttccca
    46501 atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat gcttcaattt
    46561 ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg attccattat
    46621 cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg
    46681 tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg atacccaccg
    46741 ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt gtatcccgcc
    46801 ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca taacctccgc
    46861 accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca ccgcgtacgc
    46921 cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt aattttctga
    46981 ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt ctgtacttgt
    47041 ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg atggttccgt
    47101 tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg tccgactgct
    47161 cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca cactaactga
    47221 ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag ttgttcgatg
    47281 attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg caagcaaatc
    47341 gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc tttctacccc
    47401 ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg gcttgatcga
    47461 atcagctctc actgttactt gcactggatc agagacgata cttaatactt cccctgctgc
    47521 attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca tcgaagcgtc
    47581 cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag tgatagagac
    47641 ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga acgttgaagg
    47701 aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta ctgtaatagt
    47761 cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta gcggaacttc
    47821 atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc
    47881 gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag tcactgcatt
    47941 ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg gcacccccgt
    48001 aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact ggccatttgc
    48061 attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg
    48121 tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag gaaagattga
    48181 atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg caagattgcc
    48241 aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat gctgacgtcc
    48301 ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat accgacagct
    48361 gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag aggaagatag
    48421 gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg cagcggtgta
    48481 ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa agcaatccct
    48541 gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc accgattttt
    48601 cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc atcttcctgc
    48661 tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg
    48721 atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac ttggttcatc
    48781 agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac aataaaaata
    48841 gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat cagtttgtat
    48901 acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag agagaacaag
    48961 tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac
    49021 ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg ttttaatttt
    49081 agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag aaaattgcaa
    49141 gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat tattcattat
    49201 attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt ttacgtcgtt
    49261 ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt
    49321 cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt ttcttgattt
    49381 tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag
    49441 tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa
    49501 cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag tccaaagtca
    49561 ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac caataaccag
    49621 aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc aagccatgtg
    49681 tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct gcttcttcaa
    49741 atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca taaacatttc
    49801 catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca ctagccgaaa
    49861 atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta ttggaataga
    49921 cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca tgatcgatcc
    49981 cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg ctgtttttta
    50041 cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat cgttcgcttt
    50101 cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca aacatgtgta
    50161 ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat acttctttga
    50221 ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca taaccaaatt
    50281 cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat tcattggtcg
    50341 tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc gcattcgcga
    50401 tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt tcgccacagc
    50461 ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac aagacagtat
    50521 tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc caattcacag
    50581 cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt gcgggacaag
    50641 cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata cggtatttta
    50701 cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct tccatttcct
    50761 cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca cattcattac
    50821 acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc cgcttttcca
    50881 tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca agtgtaccaa
    50941 cagagagttg atttccttcc tgtgcattca ctggttcaat tatttcttgt atataaccag
    51001 tgttcctttc attgattttt tcaggcatct ccaacgttct ccaatggtct gggatcgcga
    51061 cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct acgatagcca
    51121 ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc acctcatcaa
    51181 aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc
    51241 ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta atgttatttt
    51301 cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg tcgctccaaa
    51361 gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca tagctatgca
    51421 ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac gctgaacgta
    51481 tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt ttcgaatcat
    51541 aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg atagctgatt
    51601 tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg aatgtttgcg
    51661 gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca tcatccacga
    51721 tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg
    51781 ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca ccaataacag
    51841 ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga ggttctccac
    51901 ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta tgaggcaact
    51961 tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa aaacctgttt
    52021 tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca actgatccca
    52081 tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag tcataagaag
    52141 ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt aatttatcat
    52201 aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg ttcgtaccac
    52261 taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat ttctcctgat
    52321 tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc tcgtgactcg
    52381 tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg
    52441 caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata gcaaatcctg
    52501 tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca tttgtcgtca
    52561 ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc ttgtacatat
    52621 tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt gcaccagttt
    52681 tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct actactttta
    52741 ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact aattcagcca
    52801 tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca taagaaatat
    52861 atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat catccttgtt
    52921 catattctct cttattatct gttccattat aacgtaaaac atgccatttt tttcttttat
    52981 ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta gacaaacagt
    53041 ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga ggtgttttaa
    53101 catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa tcctttatgg
    53161 ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc cgtattgccg
    53221 acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg ggcaattgca
    53281 gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga acgtgatgca
    53341 ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac aaatattcga
    53401 tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg cagtagataa
    53461 actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca acgaagctga
    53521 gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct ttgatgaatc
    53581 tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata aggagcgtgg
    53641 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga
    53701 agcaactcct tcttatttct tggagttaaa cgcttctgtc ccagcctcct tattccgaat
    53761 aaatggtgtc caaaagcttg catctgccag attatctcat ttcttatgac aagctaagca
    53821 aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa ctaattctta
    53881 aattttcata caaaatttct caatttccct taacgagtaa tatttatgta cggtgattct
    53941 cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc aaaaatagtt
    54001 gcttttatgt atgctgttag ttgattagat ggtcatttgt tgagctaggt cgtttatttt
    54061 aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga tggcaaacac
    54121 aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa ctattaactc
    54181 ctctcagcta atagttgctc tcttttttat tcagattcat ttttgctgat cacagcctag
    54241 tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca ctggctgatg
    54301 aaatggatta gtagatttcg attattcaaa gcgattttct attggtggat cgtgttccca
    54361 actaataaga cggattccaa acctatcgta cagatcttct aaaccactta taaattttgc
    54421 acctatttca ggtgttttat tgcgtggata ttctattgtt acgccattaa atggatacca
    54481 attaggatta ggctctcttt ttttctgtac atctcccata gaatagttag cactttctgg
    54541 gacataatat attcttcgac catcctctaa ttcatctaat aagatatttc gtgatcgtct
    54601 atcacttgct aaatgtagac gccacgtgcg ataccatgcc atgctacctg catctgcaga
    54661 aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc catctacttc
    54721 atattctaat ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg gcttaggacc
    54781 caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta taacaacaat
    54841 aatgattaat attttgtttc tatttgataa atttctcatt attcgcacat cacctctaca
    54901 ttctcacata ttagttcgta atactccttt aatatcaaaa tatctactgg tcgtaataat
    54961 attcgctatg aattcctact actcgtataa gaaaggccaa atcatcagct tttacttgct
    55021 gatgatttgg cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa ttgttaacaa
    55081 tcggttcaga tatttcccag ttgattaagg taattccgta ttgtgtcaaa acttcttgcg
    55141 cttctccgaa attaagtaca gttcttatgt atccactttc tcctaatata aagtctacct
    55201 ctcttttaaa accttcctcg agatttctaa gggctatatg tggttttaat gacattgatt
    55261 gtacattttc ccccatataa tattgcgcac ttcctcgact aaaatagatt cctagatcgt
    55321 ccccaaaagg tagatcttct ctaccactag ctaatctact tctccatctt cgttctcttt
    55381 tcatagaacc tgcgctgaac tcacacccgt taaactcagc tatgacagta tcttcaatta
    55441 tgttaatctg tccatctagt tcgtattcaa tcctaaatgg aaattctcca taggtgattt
    55501 ccggttcggg tattttaggt gagattaatg caaagaaagg aaatattaag cttggcaaca
    55561 tcaaaaaaac tcctcctatg atcacaattc ccgatgtaat ccaaaatata atttgtacaa
    55621 ttctcattga tccattgtat tccattttat tccccctatt actattcata tccgcctatc
    55681 tatatcgatc ttccaatgca gagataattg cttccatacc ttgatttttt actgagttac
    55741 ctatgcgaga tatcattctc cactaaggtg catcatattg tctatctaac atgattaatt
    55801 ctccaatagg tgtatactcc tccaaaaaca acatttaatg catctccctc gacaacataa
    55861 gctaccatgt ttgctgtata tgtgtttgag tctaattcat aagcagataa atttactgtc
    55921 gctggattaa atataatcgc attcgtattt atagctactg aatttgctgc ttcagcacca
    55981 ccttttgaat gtccgatcat tgtgatttca atagtaggat ttacgtggac agccctcacc
    56041 gtttaccctg accattatag aaatcgctta cttttttgaa ctacgcaaca aaggatacaa
    56101 tgctcctaac ttcactgtct ccatttttgt ccactaagga atataaaaaa taggcattaa
    56161 ttcatttaat tcggtcacaa cacgttacct tttaaatcat tatactgtat ctcatacatt
    56221 attatcaata aactattata aaaatgcaaa aaatgcttta tcaatctatt ttgtaattgg
    56281 tcataatatc attgaaatca taccctgtat cgatttttcc tatgaatctc gtctatttgt
    56341 gattgacgaa aaagaggcaa gacagacact ggaatggtaa ttttacccac tattttgctc
    56401 caactatttg taatcgtacc cacttttgaa ataccgagat gatcatttta attgatatgc
    56461 tgattcccaa gcaaaacccc aagaaattaa atgctctatc gcatctagct tcttgggttt
    56521 tgcttatata aattccaaca tttaaatttt ttatgctgtt cggtctcaga aatgctgttt
    56581 ttgcttatat cacaatatca agatgtcaaa ttattcgtgt tgccgatgac tttgattaat
    56641 ttttttcaaa ttgtttatta agactaatgc tgtctttttg cagataccaa tgttcgaact
    56701 ttttaacatt atcatatgat cctgcaagtt ttaacttctt accattagat gttatcaaag
    56761 ttataattaa ccttgccttt ttcccattag gatcttttat tttatgaatt ttctgcaggt
    56821 aatctctcct aatggcagct atttttacct catcagtgct gataaaccat tgatcatcca
    56881 catacatact gcctgttatg ttgtgttttt ccatttcttc cgagaacgaa aagtttaagg
    56941 ctcgttctta cttccaaagt tgaatgagtc catggattgg acttagtgtg agaattgaga
    57001 acaacaataa gaatccatat cctcccgcaa acatccctaa ggctatactg tctctccatc
    57061 cataagtagg attagaagag aaaagagtca ccgtttgtaa taatatgaaa aggaataatc
    57121 cagcacaaac accaaagata acaactccaa acattgaatt tttatacaat gaccatttat
    57181 ttacaggctt ttctttcatt ttacttcccc ctactgttct gttcacacgg aatgaattca
    57241 aagttcataa gccctcctct ttgaacatat gaatacgact tgctaactat actttaaaaa
    57301 agcttgtttt aagcggttat aggcgagttg taatctctat tcatctcatt agcaaataac
    57361 ttgccgaaaa gtctactgaa attaaagcag atagtggaag aattatttag ccaggataga
    57421 cactatttgt gaaacacatg tctttacttt tgaaattcta tgatgaaaag ccttggaata
    57481 cagtagctct taattggtaa tactttttcc attataaata acctcttttc attttatatt
    57541 tatcccctct aaaaataata tctaatctaa tattttaaac ttatatttcc ctcgctgtgc
    57601 taatagagca ttactaaata aatagaaact agaaaaaagc attacgcctg ccagaataaa
    57661 ccaattattt gtat
//
LOCUS       DAJSVF010000008        55963 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSVF010000008 DAJSVF010000000
VERSION     DAJSVF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.008"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(94..390)
                     /locus_tag="ON550_000463"
     CDS             complement(94..390)
                     /locus_tag="ON550_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR3953576.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="ON550_000464"
     CDS             746..1102
                     /locus_tag="ON550_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3953577.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="ON550_000465"
     CDS             1112..2011
                     /locus_tag="ON550_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3953578.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGKLLDHYSNIKQVVF
                     GHTHRRFEDQLIHGAIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2528..3259
                     /locus_tag="ON550_000466"
     CDS             2528..3259
                     /locus_tag="ON550_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3953579.1"
                     /translation="MMKKEIVLTATTMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3404..5431
                     /locus_tag="ON550_000467"
     CDS             3404..5431
                     /locus_tag="ON550_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3953580.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5539..6636
                     /locus_tag="ON550_000468"
     CDS             5539..6636
                     /locus_tag="ON550_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3953581.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMRQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVIIETKITKKGHSKVLYREEKEALQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6942..8410
                     /locus_tag="ON550_000469"
                     /pseudo
     CDS             6942..8410
                     /locus_tag="ON550_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8503..9237)
                     /locus_tag="ON550_000470"
     CDS             complement(8503..9237)
                     /locus_tag="ON550_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3953582.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            9563..9973
                     /locus_tag="ON550_000471"
     CDS             9563..9973
                     /locus_tag="ON550_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR3953583.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            9966..10667
                     /locus_tag="ON550_000472"
     CDS             9966..10667
                     /locus_tag="ON550_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR3953584.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            10667..12280
                     /locus_tag="ON550_000473"
     CDS             10667..12280
                     /locus_tag="ON550_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953585.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHTFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            12270..12491
                     /locus_tag="ON550_000474"
     CDS             12270..12491
                     /locus_tag="ON550_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR3953586.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDECDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            12488..13249
                     /locus_tag="ON550_000475"
     CDS             12488..13249
                     /locus_tag="ON550_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR3953587.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPEIFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            13629..14138
                     /locus_tag="ON550_000476"
     CDS             13629..14138
                     /locus_tag="ON550_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3953588.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKIAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(14209..14748)
                     /locus_tag="ON550_000477"
     CDS             complement(14209..14748)
                     /locus_tag="ON550_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR3953589.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(14888..15499)
                     /gene="rpsD"
                     /locus_tag="ON550_000478"
     CDS             complement(14888..15499)
                     /gene="rpsD"
                     /locus_tag="ON550_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR3953590.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            15761..16612
                     /locus_tag="ON550_000479"
     CDS             15761..16612
                     /locus_tag="ON550_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR3953591.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAYGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            16640..17275
                     /locus_tag="ON550_000480"
     CDS             16640..17275
                     /locus_tag="ON550_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR3953592.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLTLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            17295..18068
                     /locus_tag="ON550_000481"
     CDS             17295..18068
                     /locus_tag="ON550_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3953593.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHKDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            18476..19273
                     /locus_tag="ON550_000482"
     CDS             18476..19273
                     /locus_tag="ON550_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR3953594.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            19251..19664
                     /locus_tag="ON550_000483"
     CDS             19251..19664
                     /locus_tag="ON550_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3953595.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            19648..22293
                     /gene="mutS"
                     /locus_tag="ON550_000484"
     CDS             19648..22293
                     /gene="mutS"
                     /locus_tag="ON550_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3953596.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAADVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            22311..24419
                     /gene="mutL"
                     /locus_tag="ON550_000485"
     CDS             22311..24419
                     /gene="mutL"
                     /locus_tag="ON550_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR3953597.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKFVEEDEKIYEEELALHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            24441..25001
                     /gene="maf"
                     /locus_tag="ON550_000486"
     CDS             24441..25001
                     /gene="maf"
                     /locus_tag="ON550_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR3953598.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(25086..27074)
                     /gene="treP"
                     /locus_tag="ON550_000487"
     CDS             complement(25086..27074)
                     /gene="treP"
                     /locus_tag="ON550_000487"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR3953599.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            27297..28010
                     /gene="treR"
                     /locus_tag="ON550_000488"
     CDS             27297..28010
                     /gene="treR"
                     /locus_tag="ON550_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR3953600.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFIEFARRRKV"
     gene            28087..28533
                     /gene="msrB"
                     /locus_tag="ON550_000489"
     CDS             28087..28533
                     /gene="msrB"
                     /locus_tag="ON550_000489"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR3953601.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            28703..29305
                     /gene="ruvA"
                     /locus_tag="ON550_000490"
     CDS             28703..29305
                     /gene="ruvA"
                     /locus_tag="ON550_000490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR3953602.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            29318..30319
                     /gene="ruvB"
                     /locus_tag="ON550_000491"
     CDS             29318..30319
                     /gene="ruvB"
                     /locus_tag="ON550_000491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR3953603.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            30348..30926
                     /locus_tag="ON550_000492"
     CDS             30348..30926
                     /locus_tag="ON550_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3953604.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            30978..31460
                     /locus_tag="ON550_000493"
     CDS             30978..31460
                     /locus_tag="ON550_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR3953605.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIGENKITNNL
                     "
     gene            31577..31951
                     /locus_tag="ON550_000494"
     CDS             31577..31951
                     /locus_tag="ON550_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953606.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(32687..34039)
                     /gene="brnQ"
                     /locus_tag="ON550_000495"
     CDS             complement(32687..34039)
                     /gene="brnQ"
                     /locus_tag="ON550_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3953607.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVDQAPIQPNYQDHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPILMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLHVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            34186..34776
                     /locus_tag="ON550_000496"
     CDS             34186..34776
                     /locus_tag="ON550_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3953608.1"
                     /translation="MTKSRLEAFTDGVVAIVLTVLVLEIKIPNPSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(34904..36253)
                     /locus_tag="ON550_000497"
     CDS             complement(34904..36253)
                     /locus_tag="ON550_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3953609.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(36421..37161)
                     /locus_tag="ON550_000498"
     CDS             complement(36421..37161)
                     /locus_tag="ON550_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3953610.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(37174..38766)
                     /locus_tag="ON550_000499"
     CDS             complement(37174..38766)
                     /locus_tag="ON550_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3953611.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(39334..39630)
                     /locus_tag="ON550_000500"
     CDS             complement(39334..39630)
                     /locus_tag="ON550_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953612.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            39806..40531
                     /locus_tag="ON550_000501"
     CDS             39806..40531
                     /locus_tag="ON550_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR3953613.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            40524..41366
                     /locus_tag="ON550_000502"
     CDS             40524..41366
                     /locus_tag="ON550_000502"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR3953614.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEEPLEESLETLRQKIDTIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            41369..41890
                     /locus_tag="ON550_000503"
     CDS             41369..41890
                     /locus_tag="ON550_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR3953615.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(42133..42696)
                     /locus_tag="ON550_000504"
     CDS             complement(42133..42696)
                     /locus_tag="ON550_000504"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3953616.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            42947..43216
                     /gene="rpsO"
                     /locus_tag="ON550_000505"
     CDS             42947..43216
                     /gene="rpsO"
                     /locus_tag="ON550_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR3953617.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            43404..45530
                     /gene="pnp"
                     /locus_tag="ON550_000506"
     CDS             43404..45530
                     /gene="pnp"
                     /locus_tag="ON550_000506"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR3953618.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            46040..46306
                     /locus_tag="ON550_000507"
     CDS             46040..46306
                     /locus_tag="ON550_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3953619.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            46483..48441
                     /locus_tag="ON550_000508"
     CDS             46483..48441
                     /locus_tag="ON550_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR3953620.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            48795..50096
                     /locus_tag="ON550_000509"
     CDS             48795..50096
                     /locus_tag="ON550_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3953621.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALVAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(50226..51596)
                     /locus_tag="ON550_000510"
     CDS             complement(50226..51596)
                     /locus_tag="ON550_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR3953622.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(51778..52101)
                     /locus_tag="ON550_000511"
     CDS             complement(51778..52101)
                     /locus_tag="ON550_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3953623.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(52428..53036)
                     /locus_tag="ON550_000512"
     CDS             complement(52428..53036)
                     /locus_tag="ON550_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR3953624.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            53366..54082
                     /locus_tag="ON550_000513"
     CDS             53366..54082
                     /locus_tag="ON550_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR3953625.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTSADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            54095..54790
                     /locus_tag="ON550_000514"
     CDS             54095..54790
                     /locus_tag="ON550_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR3953626.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHAELYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            54874..55503
                     /gene="udk"
                     /locus_tag="ON550_000515"
     CDS             54874..55503
                     /gene="udk"
                     /locus_tag="ON550_000515"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR3953627.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        18334 a        10138 c        11541 g        15950 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaataaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ttggaacaaa ttaaatgcgt ttttaggatc agaatcattc ggaaaattac tagatcatta
     1741 ctcaaatatc aaacaagtag tttttggtca tacgcatcgg cggttcgaag atcagttgat
     1801 ccatggagca atttatagtt gtcgaccatt cggttattat tatgaatggc aactgactcg
     1861 tgattttgtt ttgaaagaac agttgttaga cagctatgat cctatgaaat tacgtgtact
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag tcacattttg
     2281 atagatagga gggggactta cgaattattt taaaggctat gacagctgta tttctagatt
     2341 gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa tcatttataa
     2401 ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa gtatcgtaaa
     2461 acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata aagaaagagg
     2521 aatgtcaatg atgaaaaaag aaatcgtatt aacagcaaca acgatgttat tcagcatggt
     2581 agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata acacagaagg
     2641 aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc cagagaatcc
     2701 tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag gaggggcatt
     2761 atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta ctgcggataa
     2821 aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga catatgtcca
     2881 agtgacagat aggagaggta ccttatctgg gtggaaatta agtgtctctc aaccagaaca
     2941 gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta cgaaagggca
     3001 ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg aattaacgtt
     3061 gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag gtgtggggac
     3121 ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt
     3181 acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt ggacattaga
     3241 agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac gtagtcatat
     3301 tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg tttggatacg
     3361 aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa agaggaggat
     3421 cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag ggtactcttt
     3481 ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac tcacttcgac
     3541 attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt taagtatttc
     3601 gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac aaggactgga
     3661 agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt ctatcgataa
     3721 aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga agttactgca
     3781 gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga agattagttt
     3841 actctcggat tttacattta aatcttattt atacttagga aatcaacgag atagagcggg
     3901 agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc ccagtcaagt
     3961 tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc aaccttacat
     4021 tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt ctgatcgaat
     4081 ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg tcacgcctaa
     4141 accaaataat aataattatt ctggagagca tactggcgtg acggtagctc ctacatttct
     4201 ttctaatggc acatggcgct tgttaataat taaatgggat gcggagcaaa gaagtcttac
     4261 ttatgatttg gaaggtgtcg gtcgctcttc tcggattata cctgatctca ttaggcagtt
     4321 tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa
     4381 tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg taaaagtcaa
     4441 cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa ctatcagaaa
     4501 tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta gtatcgacct
     4561 cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa
     4621 agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt ttattgctgg
     4681 agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg aaaaagtact
     4741 cactatgcat tttgaatacc ttgaaaacga gaatttgatt gagcaatcga atgaaataaa
     4801 cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag ataaagacag
     4861 tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga agtataaaga
     4921 aactcctatt gatattgaag ggtatgtctt tgactcggat tcagcgaata cagaaggcat
     4981 cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac tttatctttc
     5041 aactgctcct caatatgtca attttggaaa acataaaata tccagtagac cattaagtga
     5101 attcggacag ttttcaggtg gattgaaaat tatagatgaa cgggcttttg gaagttggaa
     5161 attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc cagaagctct
     5221 ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta cgattttcga
     5281 aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag ctcatcgtgg
     5341 gatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg ggaaactatc
     5401 ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa caaaattatt
     5461 gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac aagatattgt
     5521 tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa acaacaatta
     5581 tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt gtcagctagt
     5641 gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa gagaaaaact
     5701 tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga attgagaaat
     5761 gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac caattcaaat
     5821 gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat ctttgatctg
     5881 gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt
     5941 gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg tgggatcaca
     6001 ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag cttatcgatt
     6061 gagaatgaat atacctatat tattgctttg ttgatgcgac agacactgaa cgaagttgct
     6121 ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa tgtcatctta
     6181 gcaaacgttc aaaatgatca aaaaacttac atcaatcaag taattattga aacaaaaatt
     6241 acaaaaaaag ggcattctaa agttttatat cgagaagaaa aagaagcgtt gcagattgct
     6301 ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac cccaggtgaa
     6361 tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa
     6421 aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga ttttacaata
     6481 gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa agacaataat
     6541 tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg catacttttg
     6601 tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa atagtaaatt
     6661 tttattattg taggatggtt gggctctttt tataataata taaaatatac cattttatta
     6721 ataaaaatgc atactagtaa atgatataat tcttataaca ggtttgatcg cgagaaaggc
     6781 caaataaaac gctctccaaa atgttaaaga aagacaggac gatgtatatt atacaaacaa
     6841 gaatcgctga caacagtgac tcggatccag gaaatcagtt ttatccccag ctagttggtg
     6901 aatgcatgga aagtaatata cgtagaaaga gctgatgact aatgaattat gagttttttt
     6961 taagcaaaga agatcacaga agttattcat tacttaaata tttagaagca agctcaaatt
     7021 tatcagagtc aatatcgaat gttcaagaag agttgtcgct atcgacattt ctcttgaaaa
     7081 aaacgatcga taagctgaaa catgatttac aaaagttcga tctagagaaa aatttgcgag
     7141 taaccgtcag tgaattggat atatgtctgg aaattaatgg aaaatgttct agcaaagctc
     7201 tgttatctcg ttatataacg gattctttat ctatgaaaat ggtattggct ttctttcagg
     7261 aaaaatatat ctcagtagaa aattttgcta aacagcatca cgtgagttat tctgtggctt
     7321 ataaagtatt acaaagacta aaaagaaatc taaaaaaata tcaaattttt ttgaaaagag
     7381 aaagttgaca gggaatccta aaaacattcg gcttttttta tataatcttt ttaactcaac
     7441 agagataagt tttgtggaat tgtatacgct acctataatt ataaaaacag aaaaaatgtt
     7501 aaaagattta aaacagtatt atcctctgtc tgcttatgaa aaaaagaaat tattccactt
     7561 tttagcaatc aacctgttgg ctgaacaagc tgattcttta gatattgcta gcaatcagtc
     7621 gctatttttt tcggatatag gattttacca cttaaaagga gtattaggag aaaaatccgt
     7681 tatttttatc aataagacac tctcttggct ttatcttcat gataaattgg atcaaaaata
     7741 cttgagaaaa aatgaagatg aaacaatcga atggttgaat gaacgattca ttgaaagttt
     7801 tgaaaatcag tttaatctgt tgcgagaaga gacgagccaa ttgatccgaa gagagttaac
     7861 caaaatacat tttaaacttt tgcattacac tattaataaa gctgatgaat ttccacttga
     7921 tgtttctttt tttagagaaa actatccagc attttattgc tatttgatag agtatataaa
     7981 tgatctaacg tcaaaacgca aagagttgct tcaagataaa ttttttcttt ttttcagtta
     8041 tttattatta ttgatcaatc acatacccat tcaactgatt tctgaaccag taaagatcat
     8101 catcgatttt tcttatggtg ctgcctataa tcaatttatc aagaaaaatt taagcttgta
     8161 cattaatctg aacacggaag tgatagaggc aggaaagcct gagttcccgg atgttataat
     8221 cacgaatagg aataatctat atgaagaaag tgcatctcaa gtagtggttt ggctcgatcc
     8281 gccaagagca gtagactggg ggaatcttac aaagattttg ttgactatcc aagaagagaa
     8341 atacaaagac agcaaaattg acaagcaaat agacgatttt cctgaagaaa tcatcgacag
     8401 gatcgaatag tgaacttctt ttagaaagtc cgactgcttt ttctaaaaat aaaaaaactt
     8461 ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca taaataataa
     8521 tttatataag aatactgcta cgacaccagc acagataggt gctacaactg gtacccatgc
     8581 atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta caagacgtgg
     8641 agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg aagcaactaa
     8701 tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt ggaagaatgg
     8761 tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga attcattgac
     8821 gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta acacatggtt
     8881 cacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca attgtccaat
     8941 cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag ggaatagacc
     9001 attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga ttccaccaaa
     9061 aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca tccagtcact
     9121 gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac ctagaacgat
     9181 tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt aatccataaa
     9241 tacctccaaa agttataaaa atgcaaaaaa aaagaaacat caacacgaaa agcatcggtg
     9301 cagtgtttcg tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa aaaatgaatt
     9361 ccattactta taagaaacca gaccaacagt aaaactccaa ctctttttaa aagttctcca
     9421 caaatcacca agcttaacta taaatatacc aaaaaaaaaa cgtttttttt ctcaaaaatt
     9481 gtatcttaga aacagcatgt tttgaaactt tcagaaaagg tttgttagta aaatgaaagc
     9541 tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta ttattttctt
     9601 ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg gaagtatctt
     9661 aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg aaaaaatcgg
     9721 agcaactggt gattttttat tgaataatct agctatttta tttgttcctg ctggtgtagg
     9781 tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg gagcagtcgt
     9841 cgtgtgttcc atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg tagaagcttt
     9901 gctgggctat gtccgtaaga aaaaagcagg aagggtatct gtaaacgaaa tggaggaaaa
     9961 tcaaattgac tgagtttttt gtgaatccac tatttggctt aagtttgact ttaggagtgt
    10021 atgtattatc tgataaattt ttgcggaaat ataaactgcc tgttttgaat cctctcgtat
    10081 tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat aaagactata
    10141 atatcggggg caattttttg actatgctga ttactcctgc gacagtggca cttgccatcc
    10201 ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg gctgcgattt
    10261 tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc ttctctcttc
    10321 ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt tctgtcgatt
    10381 tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc tctacaggta
    10441 tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa agtccagttg
    10501 ctcgcggtgt ggcgttagga agtacttctc atgctattgg tacagcaaaa gcaatcgaga
    10561 ttggcgaaat agaaggaatc atttccggac ttgcgatatg cgtcaacgga atattgaccg
    10621 ttttattgct accgttattc tttcagcctt ttgctggctt gttttgatgc gaaaaccact
    10681 attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag acgtgtattt
    10741 gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc tcctcctaga
    10801 aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg ttcagctgga
    10861 tgcccgcagc gtgtcgaaat atctgaacga gctgtctaaa aactatcagc agtttagcgg
    10921 taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt atgatacgct
    10981 cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc tgaaattcca
    11041 gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg cgcaaaagca
    11101 ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc aactgcaaac
    11161 ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag aaatcattcg
    11221 actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc catttgaaac
    11281 gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt ttaatgttcg
    11341 tctgaacgag atcaaaaaaa gacgattgga atacatgcta ggtgcctttt ttttaagaaa
    11401 atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt ctgacaatct
    11461 gttgttccag cgattttgcc aagtcatgga gccggtattt cctaattatt ttcaagtaga
    11521 agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt atagtgatcc
    11581 taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc cctttacagc
    11641 cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc tgccagcaga
    11701 aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg cctatctttt
    11761 tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc atttagttat
    11821 tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac acaaacatcc
    11881 caatcatctg gcatttaaaa atcatacgtt tttgatgggt cggtatctga ctgtgtttaa
    11941 aacattagga gcctttactc cacagcttcc aaagatcacg attcttttaa tgacagattt
    12001 cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc gtaatgagta
    12061 tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc tgatttcgac
    12121 aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga ccgaagaact
    12181 aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa aaaaaactat
    12241 taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta ccagaattga
    12301 aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat gttgttctcg
    12361 tatccgatga tgaatgtgat ggctgtccag tgacacctta cttattgatc aaaacaagtg
    12421 acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat gattgggcga
    12481 tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta tcacaggagc
    12541 tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg cttttgtatt
    12601 tggattagat caaaagttcg gtaaaatgga agaactctct ttgcgatttc ctgaaatttt
    12661 tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg tatcgaaatg
    12721 ccaggatcgt tttggtgaag ttacgatttt actgaataca gcggggatat tggatgccta
    12781 caaaccattg atggaaactg acgaatcgct gtgggatctg atttatgaga cgaatgtgaa
    12841 aagcatgtat cagctgacaa aaatcattct tcctgatatg atccgccaaa aaagcggaac
    12901 gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga tcgcttatac
    12961 aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt acgcaggaca
    13021 gggtatccga gtaaaaggaa ttgctccagg agccattcaa acaccaatga atgcggctga
    13081 ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag tcaagcgatg
    13141 ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc aagcaagcta
    13201 catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac cttgtgtttt
    13261 tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat aaaaaaagtc
    13321 gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt tttaataatc
    13381 gttaatattc gaaactaagc ggcttttcac gaccttttga tataaacggt gttcaaaagc
    13441 ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt tttatttgac
    13501 tattcttatt acttgttgca gggcagcttt ttcactacct tctgcggcac ttcagttgga
    13561 attttcttcc catcctttgg tcgcggtcat taccaagata aaattatctt gcaaaggaga
    13621 aatagaacat ggaaaaaaat aatacccgtt cagccaaacg ctggacaacg acagatgtga
    13681 ctcggatggc gctagtcact ggtttatatg tggcagtgac gctggttttg tctgtcttta
    13741 gctttggagt gattcagatt cgtctctcgg agatgttcaa ctatctgtcc ttgtataaca
    13801 aaagatacat cgtcgcggta acagcgggag tagctttggc taatattgct tctcctcttg
    13861 gactgataga tgttgtagta ggaagcgtca gcacgctgct tgtattgctt atcaactata
    13921 aaatcactag tcgcatcaaa aacatgaaaa tcaaaatagc tgtgacagca tgcgtatttg
    13981 ctttttccat gtttacagta gctggtcagc taacgatttt atatcaacta ccttttttct
    14041 tgaattggtg ggtgattgct ttaggagaat tggcatcaat gattgtcggt gggataatta
    14101 tttattggat cggtaaaaaa gtggatttgt caaaataaag atctgtttaa tttaaaatat
    14161 ggcagcatgg aagaagactt tgagtcttct tccatgctgc ttttttgatt aatttgtatg
    14221 aagaaatcga tttagccgtt ttttcatcaa gataccgata taagctgctc cagttgcctt
    14281 gattgcttcc ggcagtaaaa acggcagtag tccactggca aatgcagttg taaaagtaag
    14341 atcaccactt attttcagcc agatcgttcc gcagatcaat gcagcaagag agcccattag
    14401 attggcaaag attgcataca agtaatgagc atccgttctt tcgatcatcc aaccagttat
    14461 ccacgtttgt aagagaaatc caatcaaata tccgccagtt ggcccaaata aaacagctat
    14521 acctccagtc atccctgaaa atacaggaag accaatcaag ccaagtaaaa gataaactgc
    14581 aacagatatc aatcctagtt ttcttccgag aaaagttgct gttaatccaa tagcaaatgt
    14641 ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc tgagaaagga cagaaataat
    14701 cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt cggttcataa cgccctcttt
    14761 tcgttaacta aatattttta tttggttaac gaaattgtaa aacaaagtga ttactttgta
    14821 aatagaaaat ataaaaaaag accagctatc caatagatag cggtcttttt caagaaagct
    14881 tttgaaatta taagctttgg ttgtagtatt caacgacaag agcttcgtcg atttctggat
    14941 atagttcgtc acgttctggt aaacgagtca atgaaccttc caatttttca gcgtcaaagc
    15001 ttacgaatgc tgggcgtcca acagttgctt ctacagcttc ttttactgtt acgatgtttt
    15061 gtgatttttc acgaactgag atcacttgac ccacttctac gtggtatgaa gggatatcaa
    15121 cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa ttgacgtgct tgacgacgag
    15181 tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg ttgttctagt aagatcatga
    15241 agttaacacc atgtttacct tctttgattt tgctagcttt tacgaacaag ttacggaatt
    15301 gacgttcgtt cattccgtac atgtgacgaa gtttttgttt ttcagttaat tgcatgccgt
    15361 attctgattg tttgccacgg ctgttaggtc catgttgacc tggtgcgtaa gggcgacgtg
    15421 ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg acgagaaatt ttccatgatg
    15481 gtcctgtata acgagacatt aaaatttcct ccaatgaata taagtttttg gagtaaaata
    15541 atccgttgaa aaattcataa tcatatagtt tgttcttcaa tcttcacctt tgcagccgcg
    15601 gttacacaat cgaacccgag acggggcaac aaactaggga tgagttatta tttttctgct
    15661 gcattatttt acacaaacta cagtatatga tagatcatcc atgaatgtca agataattag
    15721 tacataaaaa cgctttacaa acctaggctg tctttataaa atgagagggt atgtgcaaat
    15781 ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat ccggtatcac cattatttga
    15841 gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt gaaaacagct tttcacgtta
    15901 cgctgttcga gcggttctcg cctgtttgtt tttgacttta ggaacggctg tggcatttgc
    15961 gatcgcgatg aagggggaag atatcgcgta tggtctagga aagattttat acgcatttat
    16021 gttcagctgg ggtctggtca tgattctata catgaacgcc gaattaggaa catcgaatat
    16081 gctttatatg accgtgggtg tttttcggca gagacttgct ttaagtaagg cagccaagat
    16141 tctatttgcc tgtatttttt tcaatatgat tggcggtata ttatgtggat ttctaatttc
    16201 tatgactacg ccatttcaag atttaacgtt ggacaacttt atgttagaat caatagcagt
    16261 aaaactagca aaacctgcag caacgatttt ggtggaaggg atgtttgcga atatcgttgt
    16321 caataccgct gtactgatca gcatgcggat gaaagaggat gccggaaaag tgatcacagt
    16381 cgtattcatc attttcattt ttgctttttt agggtatgaa cacgtgatag cgaattttcc
    16441 tgcctttacg cttgcttact ttgcttctca cggacagatg gatggaatga cagtcagcaa
    16501 tgtgttacat aatttgtttt ttgctttggc aggaaattat attggtggcg gtttagtaat
    16561 gggactggga tatgcttggc tgaatcagtc aaaatcatcc tatgtggact aaaaagtcgt
    16621 tcattaaagg ggaagtgcca tggaagtttt agtttttcca aaaaatgagg aaaaagcgtt
    16681 acaaatggaa aagtacatga aaaatctttt ttcctttgct ggagtaccta agccggaacg
    16741 cgctctttta caacaaccgt tactggatga gagcaaaaat ttatctattt ctgaagtttt
    16801 agcttttgtt cattattatt atgaacaaat cgaaagagag tatcagtatg tagcgatcga
    16861 tttagtccag aaaaacatca aacggcttac atttgaagac ttagtcgtat tgttgacact
    16921 agttcagaaa aaagaatggt gggacagcat cgactcttgg agaaaagtat acagcacatg
    16981 gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa ttgtttgaaa aaaaggaaga
    17041 tttttggcta cgtcgaatag caatcacgtt gcaattaggt tataaacagc aaacagacac
    17101 aggactattg atccgagcaa tagacgaaga tcgtcaaaca aaggaattct ttatccaaaa
    17161 agcaattggt tgggcattac gcgattatag taagaccgat gccgattggg taaaaagaca
    17221 gctgacaaca gatttgagta agttagctgt tcgagaggca agtaagtatt tataagaaag
    17281 cgagacgaca agagatgaga cgcaaattat ttgcttttga tattgatgga acgttgttag
    17341 attcaaacaa acaagcttta gatagcacga gagaagcatt ggaaaaatta cgcaaagccg
    17401 gtcatctggt cacgattgct acagggcgga gccgatttca tgcgcaagag atcattcgcg
    17461 atcttgattt tacgaactac attttatgta atggagcggc cggtttttta gaccatgaac
    17521 aagtatataa gaatttacta gatcgcgacc agttggatgc gttcgttcaa gaagcgcatc
    17581 atgagcagat cgatacagct tttgtcagct tagacaatat caagcgttct tcttccaatg
    17641 atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc tgtcttgcct gaattagatc
    17701 agttttttgc agaggaacaa gaaatatacc aagcgttagc atttttcgat caaacatatg
    17761 atgggaagtt ctcttcatat gataaaattc gatttgtccg ctggcatgag aacagtgtag
    17821 atgtcgtacc taaaaatggt tctaaagcag cgacaattct gcacttagca gaacaagtag
    17881 gcatccctca taaagacatc atcagctttg gcgatggaca aaacgaccgt gaaatgttgc
    17941 gcatgtcagg aattgggata gctatgggga atgctgtgcc agaagtacaa gcagaagcta
    18001 agatggtcac tgatacaaac gatcaggatg gaatttggaa ggcattgaaa gagttaagcg
    18061 tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag taataagccc agccaaacaa
    18121 aaatagctcc tcctattttt cgtttttctg ggcttattac cgcagatgca agcttttgaa
    18181 caccgtttaa ggaagaggtc gtgacagaag tggtcagttt caagaaataa gaagggattt
    18241 acgaaaattg cttctcaaat ttttgtaaaa ttacggctta tttctgaaga agtagatgaa
    18301 acaacgttta ttcgggaaat gggactgtga caagatgttt gtcatagtcc cattttttta
    18361 ttttattttt caaaactaag aaataccagg aaagagttcc ttttgtttga ccttttcttt
    18421 cttattgaaa acaactataa tgtagagtga tgttaaaaga gaagaggaat ttagtgtgcg
    18481 ggtattgttt attggtgatg tagtaggttc aatgggacga gagatgatta ctgaatatct
    18541 tcctagattg aagaagaagt accgtccgca agtaacgatt gtaaatggtg agaatgccgc
    18601 ttctggacgt ggaatcacag aaaaaatcta taaaaaattt ttgcaagacg gcgtagatgt
    18661 cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc tttgagtttg taaatgaagc
    18721 taaaaagatg gttcgtccgg caaattttcc ggaagggacg cctggacaag ggatggtctt
    18781 tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg caggctcgtt cttttatggt
    18841 ggacttggat gatccttttc gtaaaatgaa tgagctggta gaagaagcca gaaaacgtac
    18901 gccgatcatt tttgtagatt ttcatggaga aacaacaagt gaaaaacaag caatgggctg
    18961 gtttttagat gggaaagttt cagcagtggt cggtacacat acacacgttc agacaaatga
    19021 tgcgcgaatt ttgccaagag gaacagcgta tctgacagac gttgggatga caggtcccta
    19081 tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa aagtttctga ccgcattacc
    19141 aaaacgtttt gaagttgtgg aacaaggccg atcgatttta agcggctgta tcttggaatt
    19201 ggatgatacg acaggtcatg caaaagaaat ccagctgatt cagatcaacg atgatcgacc
    19261 gttcatggag tagaaagaga ggcagttttt tgcagcagga gccggaaatt caaaaagaag
    19321 taaggaaact gacaaaactt ttaagagaga atgaaacgat cattcgctac aaagaacttg
    19381 aagaaaagat ccagcaaaac cagtatttag ctgagttgag agaaaaaatc aaacaagcgc
    19441 aaaaagatgc tgttcatttt gcccactatg acaaaccagc agcagaacaa gaagcaatca
    19501 aacaagcgga tcaattcatg caagaatttg atcagcatcc gcttgttgta gcttatagaa
    19561 aacagctgtt ggaagcagat gatctgcttc atcatttaac aactatgata caagaagaaa
    19621 tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa ctaaaaatac tcctatgatg
    19681 gaacaatatc taagcatcaa ggcccaatac caagatgctt ttttgttcta tcgtttagga
    19741 gacttttatg aactatttta cgaagatgcg atcaaagcgt cacagctttt ggaattaacg
    19801 ttgacgagca gaaatcgaaa tgcagaagaa cctattccta tgtgcggtgt acctcaccat
    19861 gcagcacaag gatatatcga tgccttgatt gaaaaaggct ataaagtagc gatttgcgag
    19921 caggtagaag atccaaagac gactaaaggg atggtcaagc gcgaagtgat ccagctagtc
    19981 actcctggta cagtcatgga cagcaaagga ttgtcggcaa aagacaacaa ttttttaaca
    20041 gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg ccgatttaag tacgggagaa
    20101 ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa atgaagcctc tgcattgcag
    20161 acaaaagaac tcgtattagg cagtgagatc actgataccc ttcgtgaaca gctaagcggg
    20221 cgtttagggc tagtcttttc tcaacaaaat gagatggaag aaaacgcgga atttagcttt
    20281 ttgacaagcg aactagtcga tccgctggaa aaagaagcaa ccggcaaact attgacttat
    20341 ttatcggtga cacaaaagcg gagtttagct catatccaaa aagcagtaga ataccaacca
    20401 gatcattttt tgaaaatgga ttattattca aaattcaacc tagaattaag ccaatctatt
    20461 cggactggga aaaagcacgg cactcttcta tggttgttag atgaaacaaa aacagcaatg
    20521 ggtggccgtt tactaaaaca atggctagac cgtccgctta tccaggcaaa acaaattaaa
    20581 gcaagacaag aaatggtcgc ttctttgcta gacagctatt ttgaacgtat cgatttgcag
    20641 tcagcattaa cgaaagtcta tgatctggaa cgcctagcag gtcgagtagc tttcggtaat
    20701 gtcaatgggc gagacttgat ccagctgaaa acttctttag aacaagtgcc aatgatcaga
    20761 gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc tgctttcaga aatgcagccg
    20821 atggaccatc tcgtccaatt gatcgaccaa gctatccaag atgatccgcc gttacaaata
    20881 acggaaggaa acatcatcaa agatggattc aatgagcagc tcgatatgta ccgttctgct
    20941 atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa aagagcgtca agaaacagga
    21001 atcaagaact taaaaatcgg atttaaccgc gttttcggtt attatataga aattacaaaa
    21061 gcaaatcttg gaaatgcaga actggagaag tatgaacgaa aacaaacttt agcaaatgca
    21121 gagcgtttca tcacaccaga actaaaagaa ttggagacac aaatcctcga agcagaagaa
    21181 aaatctgttg atttagaata tcagttgttt ttagctgtga gagaagaagt caaaaaggct
    21241 atccagccgc tacaagtact tgcaaaagca atcagtgcag cagatgtgct acaaagtttt
    21301 gcaacgatca gcgaacgcta tcagtatgtt cggccagaac ttgtcagtga caagcatcag
    21361 cttctgatca aagacggacg acatcctgta gtagaaaaag tattaggaca tcaggaatat
    21421 atccctaaca gcgtagaaat ggcagaagac gaaatgatct tgctgattac aggaccgaat
    21481 atgtctggta aaagtacgta tatgcgacag ttggcattaa ctgttctgat ggcgcaaatg
    21541 ggctgtttcg ttccggcaaa acaagcagtt ttacctattt ttgatcggat ttttacccga
    21601 atcggtgcaa gtgacgactt aattgctgga caaagtactt ttatggtaga gatgatggaa
    21661 gccaatcaag ccctgcgtta tgctacgcca aacagtttga ttttgtttga cgaactaggg
    21721 cgaggaacag caacctatga cggaatggcg ttggcccaag caatcatcga gtatatccat
    21781 cgtcatgtac aagcaaaaac actcttttct acccattatc atgaactgac ggtacttgag
    21841 aaagaacttc cgcagcttaa aaatgtccat gttggagccg ttgaaaaaga cggagaagtt
    21901 gtcttcttgc ataaaatgat ggatggacct gcggataaaa gctacggcat acatgtggca
    21961 aagatcgcag gattgccttc caatctacta gaacgagcag cagatatcct gcatacgcta
    22021 gaaaatggcg ataacggaca ccatactgtt ccagctgaag tagaagagaa aactaaaaca
    22081 gccaaacagg agcaaataaa accacctgca gaaaaagtaa atcaagacca tatacaagac
    22141 cgtgtaaaag aagaaaacca acagctttct ttattcagtg aattatccga aaatgaaaca
    22201 gaagtcattg aatcactgaa gcaaataaat ttactagaaa tgacgccaat ggatgcacta
    22261 aacacattat atgaattaca aaaacaactg taggaagaag gcggatatcg atggccaaaa
    22321 tccaagaatt atctgaacgt ttagctaatc agatcgctgc gggagaagta gtggagcgac
    22381 cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga cgccggcagt actcagattg
    22441 atatcttagt tgaagaagcg ggactgcgga cgatccaagt gattgataac ggagaaggca
    22501 ttttggcaga cgacgtagag aatgccttca agaggcatgc gacaagtaag atccataatc
    22561 gcgacgatct tttccgcata cgaacgcttg gatttcgcgg tgaagctttg ccaagtattg
    22621 cctcggtttc tgaaatgact ctggaaacgg caaatcagga agaaaaacaa ggcacctatc
    22681 tgtcgctgaa aggcggagaa gttattgagc atcgaccagc accactgcgt caaggaacga
    22741 agattaccgt cagcaatctg tttttcaata cgccagcccg cttgaaatac gtgaaaacac
    22801 tgcacacaga attagcgaac atcggggaca tcgtcaatcg actggcattg agtcatccat
    22861 cgattgcctt tcgtttagta catgatggaa acaaaatgct ggcaactgct ggaaatggcg
    22921 atctgaaaca aacaattgcg ggaatctacg gattatctac cgcaaaaaaa atgctgaaaa
    22981 tcgaaggaaa agatctagat tttgagttat ttggctatgt ttcattgcct gaggtgactc
    23041 gtgccagtag gaattactta tcgacgatca tcaatggacg ttttatcaag aacttttcct
    23101 tgaataaagc aatcgttgca ggttatgggt caaaattaat ggtcggtcgt ttccctattg
    23161 ctgtattgga aatcaaaatg gacccgcttt tagtagatgt aaatgttcat ccgacgaagc
    23221 aggaagtccg gctgtcgaag gaaaaagaac taacagagct aattagtaac gccattcaaa
    23281 aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga taatctacgg tttaagaaaa
    23341 aagtacccga tcagcctaaa acagaacaaa tcgaaatcga tttgtcctac gcttttgaag
    23401 aagaaaaacc taaaaagcca agtagtttga attttgatcg agagacagga acctttttcg
    23461 tagaagaacc aaagaacaca tttgttgaca gaattgttga taagtctgtg gaaaatgttg
    23521 aaaactattc tggtttttct gaagaaaaca ccacttatcc agaaaaaaca gcgaaaacgg
    23581 tggataagtt tggtgaaaat gttgataact ttgtagaaaa aacagtggaa aaatctgaaa
    23641 agaaagaatt tcccattgtg gataacatgg agaaaacaaa tgaagaacaa gcagataaat
    23701 tcgtagaaga ggatgaaaag atttacgagg aagaattggc gctccatcct gaatttgatt
    23761 tcactcaagg aaacgacaaa acattagaac aagccatcaa gaaattagaa acggaaaaac
    23821 cagtacaacg ttttcctcag ttggaatatt ttggacagat gcacggaacg tatctttttg
    23881 cccaaagtag cgatgggttg tacatcattg atcagcatgc tgcccaagag cgcattaagt
    23941 atgaatattt ccgggaaaaa attggggaag tatctgatga tctgcaagaa ttgcttgttc
    24001 catttgtgtt agactatcca aacagtgatg ctttgaaatt aaaagagcaa aaacacttgc
    24061 tagaagatgc agggatttat ttagaagaat ttggtcaaaa cagctttatt gtacgggccc
    24121 atcctacttg gtatcccaat ggagaagaag aatcgattat tcgggagatg atcgatatgc
    24181 tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga agcaacagca atcatgatga
    24241 gttgcaaacg atcgattaaa gccaaccatt atttgaatga agcacaagca cgtgtgctgc
    24301 tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc ccatggccgt ccagtattga
    24361 ttcactttac caattcggat atggaaagga tgttcaaacg aatccaagac ccgcattaag
    24421 tcaaagagaa gagggaaaac atgaagattg tattagcatc ccaatcacca agaagaaaag
    24481 agttattggc tcatatcgtt tcagaattcg agattgaacc tgctgatatc gatgaaacgc
    24541 catttccaga cgaacagccc gtcgattatg ttcgtagaat ggctgaagaa aaagcacgaa
    24601 ctgtctggga acaagatgaa aaagaagacc atttagtgat tgccagtgac acgactgttg
    24661 ttctcaatca agagatcatg ggtaaaccag aagatctggc tgaggctgaa ttcatgctga
    24721 aaaaactaag cggggaaaca catactgtat atacagctgt tgttttaaaa acaaaggaac
    24781 gagaagaacg gatactagca gaagcacatg tcacttttta tcctttgacc gacgaagaga
    24841 tcaaacgcta cttggagacg ggggattatg cggataaggc aggggcatac ggcatccaaa
    24901 atgctgcgag tgtattcgtc aaagaaattt ctggagatta ttacagtatc gtaggatttc
    24961 cgattggtgc tgtcaatcag gcactgaaaa cattcattta aacaaaaaag cggactatct
    25021 gattttcgat tgaaaatcca tgatagcccg ttttttttat tgaaaagatt acttatctac
    25081 tctgactaag attttacttc agctttaccg actgcttcgc ccgggcgaac aagtcctgat
    25141 tggtcaagag aaagtcctgc aactttttca ttattggtca aagcaacaat aatgtcagtt
    25201 ttcttgccag cttgttctat ttctggcaaa tccatttcag caatcagggt ctctggtgtg
    25261 acaaggtatc cttctttcac gaatacattg aaaggaacac cctttagttc aacagtatct
    25321 aatcccatgt gaacaagtac ttccagcccc tcattagaaa gaataccaat ggcatgtttt
    25381 gtttcaaaga cactcgtcac tttaccgttt actggtgcat agactttgcc gtttgacggt
    25441 tcgacagcat aaccgtcacc catcattttt tgtgagaaaa ctgggtcatt tacttcagtt
    25501 atttccttga tggtccctgc agcgactgcg aataatgttt cttttgttgc aactgccgta
    25561 ccagttgctg aaacaggttc gaaagactgt ggcgttgctg ttgcgccatt tgctgtttga
    25621 agttgtacat cagcagcatt ttcaggaaca gcaggatcaa tcttattcaa aattccctga
    25681 cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag caatgatcat tgcgatgatg
    25741 aaaggtaccc atgtttctgc ttggatagct agtattcctg gcaaaccgcc tactccgatc
    25801 gcgttagctc gaacacccat caagttagca aacatgccag ctaatccaga gccgaccatt
    25861 gcagcgacga aaggatagac gtatttcaaa ttgattccga acatagcagg ttcagttacg
    25921 cctaagtaac aagaaatcat tgctggaatc gagatttgtt cttctttttt attcccacga
    25981 tgtaagaaga tgatggctaa gactgcagag ccttgggcga tatttgataa agcaatcatc
    26041 ggccataaat tcgttgatcc gaaatctgca atcagctgca tatcgatggc attggtcata
    26101 tggtggagac cagtaatcac taaaggagca tacaggaatc cgaatacggc actaaacaac
    26161 cagctgatag aagacgtcaa tccagtattt acgatatttg agatccagct tccgactgtc
    26221 cagccgatag gacctaaaat aacgtgtgca gctaaaacag ttggtaacaa tgaaaacaat
    26281 ggaacaaaaa tcatcgagat cgactgaggg atatatttgc ggaagaagat ttctagatac
    26341 gctaacataa accctgccag catggctgga ataacttgcg cttgataacc aatcatctgg
    26401 acttgagcaa aaccaaaatc ccaaaatgga atatctgctg cggctgtgga agctacacta
    26461 taagcattta ataattgcgg agaaactaac gtaattccta aaacgattcc taatatttgt
    26521 gtagtaccca tcttttttgc aatactccaa gtgataccta ctggaagaaa atggaaaatt
    26581 gcttcacccg gtaaccacaa aaaggcattg acaccattcc aaaacttcga gtgttcaacg
    26641 attgttccac caagactttc aaactggata ccttcaagta catttctgaa tccaaggatc
    26701 aatccaccga caatgattgc aggaatcagt ggtgtaaaga tttctgctaa aacggcaata
    26761 gcacgttgaa cgggatgaag gttttgtttt gcagctgctt ttccttgttc tttggaaacc
    26821 ccttcaactc cagatactgc aacgaagtcg ttataaaaag tagaaacatc gtttcctatg
    26881 atgacttgga actgtcctgc attcgtgaac atccctttta cgctaggtat atcttcgatc
    26941 gctttttcgt ccgcttttcc tggatcattc aggacaaaac gcatacgagt cgcacaatga
    27001 ctaactgcag caatattttc ttttccgccg atatcatgta acagcttctc ggcatctgct
    27061 tgatattttg ccatatcaat aaaccccttt ctcttaactt gtctatgcaa gttgtttctt
    27121 catttactat actttttttc ggagagaatt gcaagcgttt tattaaaaag tacgagagaa
    27181 aatgatatcg ttttaaaaag cttaaaataa gcttttctta aaaataggaa cattgaaata
    27241 caaaaagagc tatagtatag tgaagaaatg atggaagaac gagtgaggga aaaaacatga
    27301 ataagttcaa tgaaattttt ttagatttgg aacagaaaat tttagcgaaa gaatatcctc
    27361 cgcattcgct tttacccagt gagaaccaat tgatcaaaat ttatggtgtt tcacgagaaa
    27421 caattagaaa agcgctgaat cttttgacta cggaaggtta tattcaaaaa aaacaaggaa
    27481 aaggttcgat cgtactggat cggaatcgct tcgactttcc tatttctgga ttgactagct
    27541 ataaagagct acaggagacc caacgtattc caagtgaaac gatcatccat acgttagaag
    27601 aaacagaagt gacaaaagca ttgaacgaga tcactggctg ggagatcggt gcgcctgttt
    27661 ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt tatattagac accgattatc
    27721 tattaaagga aatcgttcct attttaactc cagcacaagc acaaggatcg atctatgaat
    27781 attttgaaaa tgagctttct ttgacgattg cttacgcgca aaaggagatc acagtagaag
    27841 aagtaacgga aatcatgaaa acagcgatgg atcttcacga tgacacttat gcagtagtag
    27901 tcagaagcct cgttcatctg gaagataccc gctgtttcga gtattctgag tcggttcacc
    27961 gattagataa attccgtttc attgagtttg cccgacgccg aaaagtgtaa atagttcaaa
    28021 agaaagcaaa ttgttggcat ctgtccactt tggcgctatg ataggatcac aaagggggct
    28081 tttttaatgg ataaaccaat aagaccagaa aaagaagaac tgaaagaaaa gttatcaccc
    28141 atggcttatg cagttacaca agaaaatgca acagaacgac catttagcgg gaaatatgat
    28201 gatttttacg aaaaggggat ctatgtagat atcgtcagcg gtgaaccgtt attcagctct
    28261 gcagaaaaat atgatgcagg ttgtggctgg ccatcattca gcaaaccaat aacgaaaaga
    28321 caagtgcgcg aaaaagccga tttttctcat ggcatgcatc gaatagaagt tcgaagcaaa
    28381 gaagcagatt ctcatctcgg acatgtgttt accgatggac cagtagatca aggtggtcta
    28441 cgttactgca tcaattctgc ctcactgaaa ttcattccat atgaaaagat ggatgaatta
    28501 ggttatgggg aattcaaatc acttgtagaa taatggaaat acacaaagag cggtacaata
    28561 cggggattta ctcgatcgtc cgctcttttt ttattcaagc gattgctatt tgaaagaaac
    28621 gctccccaga ctcctttttt cacaaggtga aatgtgctac aataaaggga acgtatgtgt
    28681 gcaaaagaaa gaggactaga atatgtatga atatctgaca ggtgtggtta cctttatcaa
    28741 cccctattac atagtagtag aaaccaatgg tatcggctac cagatcgcac taggaaatcc
    28801 ttaccgctac agtagcaaac taaatcaaaa aataaaaatt tatgtccatc aggtgatacg
    28861 tgaagatgcg catcttctat atggatttga ttcgctagaa gaaaagcaac tgtttttgcg
    28921 attagtaagt gtttcaggaa tcggtccgaa aagtgccctt gcaatcatgg caagcgaaga
    28981 ccactccggt ttgattcaag cagtagaatc cagtgatgtc acttatttga cgaaatttcc
    29041 aggagtaggg aaaaagacag cacaacaaat gatcttggat ctaaaaggaa aattcggtga
    29101 gctgagtatc gatacacctt ttagtctatt tgatgaagca aacacaaaag acgctacagc
    29161 tctatctgaa gcaatggaag ctctctcggc tcttggttac agtgatcgag aaatcaaacg
    29221 tgtagaaaaa caattaaaag aagtcgataa tcagacaacg gatgagtatc tacgccaagc
    29281 actcaagcta atgatgaaaa aataaggagg agaccaaatg acagatgaat acttgttatc
    29341 accagaatct ggcgaaaatg aactgagttt ggaaaaatct cttcgaccac agctgctttc
    29401 tcaatatatc ggacagagaa aagtcaaaca agaacttcag atttacatcg aagctgccag
    29461 aaatcgggaa gaacctttag atcatgttct gttgtacggg cctcctggat taggaaaaac
    29521 gacgatggcc atggtcattg ctaatgagat gaccgtaaat attcgtacga cgagtggccc
    29581 tgctatcgaa cgtccgggtg acttagtagc aatcttgaat gaactggaag caggggatgt
    29641 cctgtttatc gatgagatcc atcgattacc tagagtggta gaagagatgc tttattcagc
    29701 tatggaagat ttctatgtag atatcatggt aggacaaggg ccaaccgcac atccggttca
    29761 tttccctctt cctcccttta ctttgattgg cgcgacgaca cgggcaggta tgctttctgc
    29821 acctttacga gatcgatttg gcatcatttc tcatatggaa tattatgacg aacaggatct
    29881 gcaggagatc gttttacgtt cttcggatat ttttcaaaca gagatcattg aagaaggggc
    29941 attggaaatt gctcgccgat cacgaggaac gcctagaatt gctaatcgct tgctcaaacg
    30001 ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc gatcgtccta tagcggatca
    30061 ggcacttact ttgctacagg tcgatcaggc tggattggat tatgttgacc agaaattgtt
    30121 gcgaacaatg atcgagttat atggcggcgg accagtaggg ttaagtacgc tttcagtaaa
    30181 cataggagaa gagcgtgaga cggtagaaga tatgtatgaa ccgtatctga tccaaaaagg
    30241 atttttaaag cgtacaccta gagggcgaat tgctactgct tatgcctatg aacattttgg
    30301 ttacgactat catatatgat ttttgctttt agattggagg aaaatgaatg agtgatacaa
    30361 cacgtacgaa acaattgatc aaaaacgtat tgattgattt gtgccaaacg caatcttttc
    30421 gaaaaatcag cgtccaaaaa atcgcaaatg aagcaaaaat caatcgacaa acattttatt
    30481 atcattttac agataaatac gatttgcttc gttgggtcta ttgtgaggat gcattgcatt
    30541 atttagatcc acaatgtctt tcacttgata attgggaaga gcaagcattg aaaatgctga
    30601 aagcaattgc tgagaagaaa gaattttatg caaatactgt gctttctgac ccagaaattt
    30661 tacaaaaaga gttttctcaa ctgatccaaa aatcatttat caagattttt gaacaaatgg
    30721 atgaagaaag acaactttct gcaagagaca aactatttta tgctcgtttt ttttcttacg
    30781 gttgcagtgg tgtattagtc aattggatca cgaatgaata tgaagaaaca ccgatggaaa
    30841 tcgctatgca gcttttccgt ttagccaagg atacggaatt atattcttat cgtctttatg
    30901 caaaagaaga gttggaagaa cagtaataga aaaatttttt gttggaaaag caatgatcgc
    30961 taaaaggaga tggggaaatg acaaaaattt tatttgtttg tttgggaaat atttgccgtt
    31021 caccgatggc agaaggcttg ctgagaaaac gaatagcatt agaaggaagg gaagcagaat
    31081 tttttgtgga ttctgctgct acaagcacgt atgaagttgg aaagacaccg caccctggaa
    31141 cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa tatggttgct cggcaaatca
    31201 caccccacga ttttgaaact tttgattgga tcattggcat ggatcaagaa aatgtagaag
    31261 aactgaaacg aagagcacca cgctcagcgc aggggaaaat ccatttgttt ttaagtagca
    31321 ttcctggaaa agaaaaagaa aatgttcccg acccatacta taccaataac tttgatgaga
    31381 catatcattt gatcacagaa ggcctgaatc attggtggaa tatttggatt ggcgaaaata
    31441 aaataacaaa caatttgtaa agttgacgct ttctttttaa ggcgatgtta gagcatttcc
    31501 ttgttttttt gtaaaatgtt aggtaaaata aaattaatta agtaacactt tcttagctaa
    31561 aggaggagtg cattcgatga acgaagagac agtatcgcca atgaagaagc ttacgaagtt
    31621 agttactcat acttcgtatc aactttttga ttcttttgta aatgacactg aagaacatgc
    31681 taagaatgta cgattgcaag gaaagtttat tcttgagctg caaaaagctg ctactgaaca
    31741 tagtctagtt atcttacaag taagagcaaa tcgtttgagt agcaagtatg aaacgatctc
    31801 aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg atgatccgcc tacaaaacga
    31861 agagcagcag atccggatgg ttcctgtaga acaaattgaa aaaataacaa tcctttcacc
    31921 agcaggggaa caggaacgat tatcaaaata atgtatacca gatagaaaag cacgtatgag
    31981 cttgtaccat caatcattac taatcaaaaa taaccgaatg ctttaagctg atatcagtaa
    32041 aacggaacgt tttaggatga gtatcacagc atgacagcat tcggttgatt agttgaatcg
    32101 attggatggt acaagttttt ttgataaaaa aaggtcctgc tagccgggga gctagcagga
    32161 taaggagtta aaaatgaaaa agtgttgtta gggttgtttg ttggtatgct tatataatag
    32221 tcattagttg tgaatatttt gtgacgaaag ttttcgaaag ttgttaagtt tttttgcgaa
    32281 aactattttt atatcatata tatgtctaaa atctatatta taaaagaaat gttgtttgat
    32341 cagcttctct tcggtattag ttcaaaacaa aacggcaaag tatatggagc tactttgccg
    32401 ttttgtttct ctagtactca ggtcagttta tagacgctca ataaacggtg ttcaaaagcc
    32461 agtttctcgg tattaagtca aatttatgca aaaatgtgag aagccatttt cacaaaatat
    32521 ttcttaatac ttgaaactaa gcggcttttt cacgacctct caataaacgg tgttcaaaag
    32581 tcagtttctc ggtattaagt caaatttatg caaaaatgtg agaagccatt tttcaaaatt
    32641 actttttatt gctcaaggct gaacgtctct ttcacaacct cttttatcaa tctgctactt
    32701 cttctttctt agcgaaactc caaatcagtc cgataacaaa accgataact gctggtacga
    32761 tccaccccat ccccaatttg aaaaacggca agtagctttc cgcaacatgt aaaagatttt
    32821 gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt caacccatca aaaatcgaag
    32881 caatcaatgt aaaataagta gtcattcgat aaacagccgt acgctgctta aacaaaggac
    32941 caactaacac tagtaaaatc agtgtcattg ctaatggata gatgaacatc aaaataggca
    33001 acgaaaattg aatgatattc gtcaagccta cgtttgccgc taaacaagga aggatactag
    33061 caagtgcaac aaagaatgcg taatttcttt gcggaaacaa atctgtgaat gtttctgcaa
    33121 aagctgtaat caaaccaata ccggtcttca aacaagctaa tataacgata aatgctaata
    33181 agatactgcc ataagtacct aaataatgat cagcaatctg agccagcgtg atcccgccat
    33241 tttcacttag agcaaagcta cctaagctca tagcacctaa ataagcgagc aaagtataaa
    33301 tgattcccat caatataatg ctgatcgcac cagatttgat cgtgtcttta gcgatttcag
    33361 caggttttgt tacacccatg ttttggatcg tcgtaacgat gatgatccca aatgctaaag
    33421 cagcaagcgc atctaatgtg ttatatcctt gagtaaatcc tgtgaaaaaa gcatggtctt
    33481 ggtaatttgg ctgaataggc gcttgatcga cagagcctaa tggattcaaa aaagcaagga
    33541 gaagcaaagc acctaataaa accaaaaatg caggattcag gaactttcca acataatcta
    33601 atatctttgt cggttttctt gaaaaccacc aagccagcac aaaaaataca atactgaaaa
    33661 ttgctagata tagcgtctgt ctgctaggat cgataaatgg tgctaaacca atctcgaaag
    33721 atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat aactaagtaa agcaagatgg
    33781 taaaaaccat cgcatatgtt tgatttatcc gttttgctaa ttcatataca cctgagcttt
    33841 tcgatacacc aatggcaatc acccctaaga agggaagacc gataccggta accaagaaac
    33901 ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc caaatgaacg ggaaagataa
    33961 gatttccagc accaaaaaat agaccgaaaa gcatggagcc tataaaaaga tagtctttaa
    34021 atgataattt ttttgtcata cataactcct ttagggtttt tttaaaagta acaaaatttc
    34081 gaaaaatatg caacaatttt cagaaaattt cctatataaa acgatggtta tttaataacg
    34141 taagtgataa aataaaagcg ctgaatcatc tgaggaggaa tcatcgtgac aaagtcgaga
    34201 ttagaagcat ttactgatgg agtggtagcc attgttttga cagttcttgt attagagatc
    34261 aagatcccta atccatctag ctttcattct ttatgggtca tccgaaatac attattagct
    34321 tataccatca gctttatatt tgttgcggtg atctgggtga accatcatcg tatatttcag
    34381 atggcagaac gaattaatta ccgagtggtc tggtcgaata tctttttgct cttttggctg
    34441 actttatgtc cttctgttac tagttgggtc gggcgtaacc ctgaagcttt ttggcctgag
    34501 atggcttatg tcttagtata cacgatgtgg agtttttctt ttggtatctt gatgaggcaa
    34561 atcatgaaag caaataaacc agattcccat gtcgtgagag ttttgtcaaa ggaccgtcga
    34621 agtcttatct ccatgatgat taacttatgc ttgattgccg gtgtcttttt tgtaccgttg
    34681 atcgggttat tcggacgttt ttttgtatct ggtatttgga ttttttccta taaaaaagca
    34741 gatgcctatt atcagcggtt gtttccgggg aaatagaaaa gaggctgaga cagaagcttt
    34801 taactccaag aaataagccg aaatttcaca aaaatttaag aagcaatttt cgtaaattcc
    34861 ttcttatttc tcgaagctga ccacttctgt cacaactact ttcttacttc ttacgttcat
    34921 atttcatatg atagattagt aatagaagca agaaccacac aggtgttagt aataaagcga
    34981 ttctcgtatc ttttcctagg aacaagacga cacacacaaa ggccatgtaa agaaatacca
    35041 gataattact aaatgggtac aacggcattt taaatgaatg agcttttttg ccgaggatac
    35101 ttttacgata tttcatgtgc gtgtagacaa gcatactcca gataaacaag aaacaagtag
    35161 tggcaatact gcttactagt gtgaatactt cactaggcat cacgtaattc aagataacag
    35221 aaatgaaaat gacgatagta gaaaatgtca aagcattccc tggcacatga tgtgttgtta
    35281 actttttgaa ttttttaggg gcactgcctt cttgcgctaa agaacgaagc atacgtcctg
    35341 tactatatat tgcactgttg caggcagaag ctgctgcact taatacgact aaattgatca
    35401 aggaagcagc aatcgtgatc ccaatttccg aaaatacttc tacaaatgga ctgttatctg
    35461 gattcaagct gttccatgga tagattgcca taatgacaaa aagagatcct aagtaaaata
    35521 agatgatcct gataggaata ttattgatag cttccggcaa cactttttca ggattttctg
    35581 tttctccagc gactaatcca actaattcta tcccagcaaa agcaaaggtt accatttgga
    35641 aagctaagat aaatcctttt gcaccagttg gaaagaatcc gccatgagac catagattcg
    35701 tgatactggc tgtgccaata tctgtcgtat agtgggtgag gatcatataa gctcctacga
    35761 ggataaaggc aatgatcgct acaactttga tcaatgcaaa ccaaaactct aactcaccaa
    35821 acaatgagac agccaccata ttcaagcaaa gaagaagacc taatgcaatc agttcaggaa
    35881 gccactgagg aacaccaggc gcccagtagc gtacatacat tccgatggct gttaaatcgg
    35941 ccattgcaat cgatacccag cagaaccaat acgtccagcc agtgatgaaa gccgcttttt
    36001 tgccaagata ttctgcaaca aaatccagaa aagaatggtt attggtgttt gataacagaa
    36061 gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc agtaataaga taggcgagca
    36121 atatcgaagg gccagctagc tggatagact ttccggaacc taaaaacaac cccgttccaa
    36181 tcgctccgcc aatagaaatc agttggacat gtctattttt cagtccacgc tgcagttctt
    36241 gctcttcatt catttttaaa tcatcctaac taaaaaagtt taattacttc catattaaga
    36301 gagttttctg aaaaacacaa ctaagattcc aaaattttca gaaatttacc ttttatgagt
    36361 aattcataaa aaagaactgc ggtaaacacc gcagctcttt gtcccaatca gctatttatc
    36421 ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt tcttctttcg gatggacaaa
    36481 gatatcttct ggtgtacctt cttctgcaat cacaccttta tccataaaaa tgacacggtc
    36541 agatacatct tttgcaaatt ccatctcatg cgtcacgata agcattgtca agccagtatg
    36601 tgctaagtct ttgatcgtat taagcacttc gccaaccatt tcagggtcta acgccgaagt
    36661 aggttcgtcg aatagcaata catctggatt cattgataat gcccgagcaa ttgcgacacg
    36721 ctgtttctgt ccaccagata attgtgctgg tttggcttcg atataacgat ccataccaac
    36781 tttttccaga ttttcgattg ctactttttt ggcagtttct ttatctcgtt tcaggacagt
    36841 tgtttgacca gacatacagt tttccaagac attcatattg ttgaacaggt tgaaggattg
    36901 gaaaaccata cctagattcg ttcgatattt aggtaaatta tatttaggag ctaatacatt
    36961 ttctccatta taaatgatct cgccgcctgt tggtttttcc agtaaattga tacagcgcaa
    37021 gaaagtagat ttaccagaac cggaagaacc gatgatcgtc acaacttcac ctttattgac
    37081 tgttacgctg atatctttca atacttcatt ttcaccgaag ctttttctta gatgtttgat
    37141 ttcaataatt ttttcattca tgattcttcc tccttattcc tgcaaatttg aattgtcgat
    37201 ttcttcaagc ttggtataag ctgaaggacc atcgattttc ttttctaccg cccgcaggat
    37261 tctggtgatc gtaaaggtca tgactaagta gatgatcccg acgatcgtaa atgtttggaa
    37321 gaattggaaa ttcgcaccgg aagcagagtt tccttggaag aacaaatcag ctacgccgat
    37381 tacacttaaa actgctgtat ctttgatatt gataacaaac tcatttcctg ttgctggcaa
    37441 gatattccgt aaaacttgtg gaataacaac ttttctcatt gtttggccat gggtcatacc
    37501 aatggcttgc gcagcttcga actgcccttc atctactgca aaaatccccc cacgaacgat
    37561 ctctgtcatg taagctccag tattgagaga gacaataaac aaagcggcaa tcgttcggtt
    37621 caatgaaatg ccaaaagcga gtgccagacc gtagaagatg accattgctt gtaccatcat
    37681 cggtgttccg cggaatactt cgacatagat agaaagaagt gcatttccga ttttttggaa
    37741 gaagcgagtc agtgcatttc ctgattcagg taccgaacgg aagacaccaa tcagtaatcc
    37801 aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac aaggtaagtc cagcgcctcg
    37861 taagaacata ttgccgtatt gtttccaaat cgtagtgaaa tcagaaatga tgccaggttt
    37921 ttcttcacta tcagttgcag caggctgatc tttgactgct tggtccataa ttttggttcg
    37981 ttcatcgtta gagatgccag aaagaatctg gtttactttt tggatatccg ggtcgccttt
    38041 acgcatacca acagaaatct gaacatcttc cgggtttgtc tggaagccat ctgcaccgct
    38101 gaattcaagc atttccaagt ctttgttcac actagttgcc gttacacctt ccggacgttc
    38161 gctgacataa ccatcgatca ttccagaagc taaagctgtt ctcattgctg agaaattatc
    38221 cattgcttgt tgtttctgca catctggtat ctgatcgatc acagtatagt ggaatgtatt
    38281 taactgagca gtgatctttg ctccagaaag atctgctaaa ctttttgctt ttgcatattt
    38341 gctatctttt tgaactacta ctactaattg tgattcgtaa taaggatcgg taaaatcaat
    38401 ttcttttcgt cgttcagcag tagggctcat tccggcgatg atcgcgtcga tcttgcctga
    38461 ttgaagggca ggagccagac catcccattt cgtttggacg atgactaatt ttttccctaa
    38521 accatctgct acttttttag cgatttgaac atcataaccg ccggcgtaac tattttggcc
    38581 ttgaattggc aaggcattat tctcatctgt ttgttgagac cagttgaagg gtgcataccc
    38641 agcctccata cctacgcgaa actcatcgct cggtgttttt tcatctgcat ttgccagaga
    38701 tcccacactg aatagtgaga agacaaaagc aaatagtacg gcaactgaaa ccatattttt
    38761 tttcattttt ttctcccttt ctgattatat gatacacgag cggtctagat gaaatgcggt
    38821 aaagctttat ctgaaaaaat aaaaagcgac cgatttatag atataaaaag gccgctaaaa
    38881 ttccataagg tgataaacct ctcacaaaac atagcgcaac ttagcatcac tgctaagaca
    38941 gtccgcaagc tcttaacttg cgtcccaaca tacactccaa gcaaaaaagt gcacatttcg
    39001 gcgatcctcc ctattcctgc ttctccatgt ttgcttcact ccacagggtt aatgattctc
    39061 gcgacctcta cctcattgat tgaggcatct cgtattcagt tatttataca aaaaaagttt
    39121 acgatactcg agaggctttg tcaatctata actgctgttt tttgaaaaaa tactgataaa
    39181 tatagaaatc tttactttta ccgaaaaaac aatcaaaatg tgaatcatct atccattgca
    39241 aacgatagta taattataca caaaaatgta tgtaatacaa taaaaaaaga actagaacag
    39301 aaaatttcct gtgtccagtt ccatctcgat catttaagct gccgctttct ttggctcacg
    39361 tcctaggatc gcttgcaaaa gaacaacgac gccataaatg gctaatatta gataaatcaa
    39421 acttgagtat tgggtgattc cccagcccca tgttcgatat acggctaatg taatggctaa
    39481 aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa agatttccat tttctttgcg
    39541 tacaagataa aaaacagaga aataaacgct taacgctaac caggcgatac aagccagaat
    39601 aacgccccaa taaataaaga atggtaacat atcgattcac ctcactttta tacagcagta
    39661 ttgtaacacg ttttcattct tttgtcagtg aacattgtga taaattgctc aaatgcccat
    39721 aacacttttt tattcccatt tttctgactt ttcaacaata aatttgttcg agtatgggct
    39781 ataatggaaa gtgaggtgac aaaacatgaa acaattaaca gtagacgaag cagcaaaaga
    39841 gatattagct tatcagcaaa ttacattttt gaccggtgca ggggtatcca caccttctgg
    39901 agtgccagac tatcgatctt tgtcaggaat ctaccacggg atggagcagc cggaatactt
    39961 gctgagtcat caagcaatgg tgaatgaacc ggataaattc tatcaatttg tcaaaaagat
    40021 ttatcatcct gaagcaaaac ccaatatcat tcatttggaa atagcacatc tagcgaaaga
    40081 aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg cacgcaaagg ctaaaagccc
    40141 gcaactcgtc aattttcatg ggagtctgta tcaatgtcat tgtcgaaaat gtcaacaaac
    40201 ggtagactgg aaagaatatc ttcacagcga caaacatcaa gcatgcggag gacaaatacg
    40261 cccagacatc gtgttatatg gtgaaggatt tcaagatgga gtcatggagc aggcggctta
    40321 tgccgtcagc caagcagagc tgatcgtcat tgttggaacg agttttcaag ttcatccgtt
    40381 ttgtgatctg gtacagtttc gccagccgtc agccaaaatc ttagtaatca atcaaacgcc
    40441 tgtttattta gcggagcctt atacgtttat tcaagaagac ggagcggttg ttttcaaaaa
    40501 aatacaagaa ttaggagcag attatgactg aattcaaaac cgaaaaagaa aaaatgattg
    40561 caggcgagct ttattttgcc aatgatcctg agctggttgc cgaccgtatg tttgcaagaa
    40621 gtcaaagcca aatcatcaac caagcagaat cagcggaact gcgatcccaa ttgttgaaag
    40681 agacttttgg ccgtacaggg aaaaaaatct atatggagcc agttatcaat tttgattacg
    40741 gatataatat atttgttggg gagaattttt atgcaaattt caattgtaca tttttagatg
    40801 tcagcacgat tgaaatcggt gataactgca tgttcgctcc gaatgtccag ctttacacgg
    40861 cgacgcatcc actacatcca gtgaaacgta acagcggtct ggagtacgca aaacccatta
    40921 aaatcggtaa taatgtttgg ttaggcggag gagttatcgt cacgccgggt gtcactctag
    40981 gggacaacgt agtagtcggc gcaggttcag ttgtgaccaa atcttttccc gacaatgtag
    41041 tgattgcagg aaatcctgct cgtatcatca agacagtaga ggaagaaccg ttagaagagt
    41101 ctcttgaaac attacgtcaa aagatcgata cgatcgatcg agagttggtt gctttattag
    41161 aaaaaagaat ggatacggta actaaaatag gacaagtgaa aaaagaagaa aaccaagcag
    41221 tttatgatgc aaaaagagag cagcaagtat tggataaagt tgttagtctg ctgaaaaata
    41281 agggatataa ggaaacaata acagacacgt atgttgattt gatgaaacac tcgcgcgaat
    41341 accaaaacaa aatgaaggag gaataagtgt gatcgaagga gagacacggc gaaaacaaat
    41401 catgcaaacc ttggcaaata cagaaaaacc agtgagtgct agcaagtttg cgaaaacttt
    41461 tggagtcagc cgtcagatca ttgtaggcga tattgcttta ctacgagctg caggtaaaag
    41521 tatcgtggca acagcaagag gttatatcct cgaaacagaa gaaaccaata ctgggcacat
    41581 cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa gaagaactga gactaatcgt
    41641 tgaaaatggt ggtgaaatca ttgatgtaat tgtggaacat cccctttacg gtgagttagc
    41701 aggaactttg cacatcaaaa cgccacaaga tatcgattcc tttatgagac gttatcaaaa
    41761 aagtaatgct actttgttat ccgaattaac aggcggcatc catctgcata cgatccgcta
    41821 cccagaaaag catatgctga aaaacatcaa acaaaaacta gctgaagcag gaatcctcta
    41881 cgaaggataa catcgtagct gctacttttt atcctagaaa ctattctatc atctctgaga
    41941 taaacaccca tatggaaaaa ataagaaaag atgcgtatcc aatacacaga attttttgga
    42001 attgcgattt ctagatgtct atctaaattt cgattagtgg agaaacataa aaaagggaag
    42061 tgcagctttt gagcactccc cttttttata aagctttctt gtagcatagt atcgaaggaa
    42121 cctgccaatt ctctactcga ttaccaatac gccttctttt agtgcaaatg gattttgatc
    42181 gttgatgtgg tcatagaaca tcacaccgtt gatatggtcg atttcatgtt gaacgacgat
    42241 cgattcataa ttcttcaaac gaatcttgtg tttttcacca ttcatatcat aataagacac
    42301 agtgattttg gcatggcgaa caacatatcc tggtacttct cgatcaacag ataaacagcc
    42361 ttcaccttcc cctaaacaag catcttgaac agaatggctc aaaattttag gattatacat
    42421 aacagtactt agagaaggtc catctgcctc tgggtcagga cttggtacgt gaacagcaat
    42481 gattctttta gaaatatcta attgaggagc agctaagcca acaccgcctc gtaagtgtag
    42541 ttcttcagct ttgattggat cttggctgtt ttttaagaac tcaagcatct cttttcctaa
    42601 agagatatct tcttcgctta atggaagaga aacttctttt gcaacttcgc gtaaggtcgg
    42661 atttccttca cgaatgatat catccattgt aatcatggaa tatgtacccc tttcttaaaa
    42721 gactgtgttt caagtctatt ttcgcattat agtttaccac aaaaatggaa aagtagtagt
    42781 aagattgttt gaaaactacc ttttcttaat gattaactac ttgattttca acccggtttc
    42841 aggtaaagta tagctgtgtg ctctaggcac atgacctgcc acttggttaa gcgaatcacc
    42901 aacgttttac tcagtcgcag gggaataatc gaaagaggtg aaagaaatgg caatttcaaa
    42961 agaacgcaag aatgaaatca tcaacgaata cgctcgccat gaaggggata ctggttctcc
    43021 agaagtacaa atcgctgtat taactgaaga aatcaaccac ttgaatgaac atgcacgcgt
    43081 gcacaaaaaa gaccatcatt cataccgcgg cctaatgaaa aaagttggtc accgccgtaa
    43141 cttgttagct tacttacgta aaactgacgt tcaacgttac cgcgaattga tcaaacgttt
    43201 aggcttgcgt cgttaatttt agtcaaaaag agtgggattc acatgaatct cacttttttt
    43261 actcgtatat caattaagta aaattccgtg atcaagctag atggactcct actaaccaaa
    43321 tgaaagctgc tcaaaagacg gctttcggca gttttcattt gtttagtagc gtcttgcttt
    43381 cacgagaagg aaaaggagaa gttatgtcag aaaaacaagt attcaaaacg acatggggcg
    43441 gccgtccctt acaaatcgaa gtcggtcagc tagcaaaaca agcaaacgga gccgttttag
    43501 tccgttatgg agatacagta gtcttaagtg cagctgtggc ctctaaagaa gcaaaagata
    43561 cagacttttt cccattaacg atcaattatg aagaaaaaat gtatgcagca gggaaaatcc
    43621 ctggaggttt catcaaacga gaaggccgtc caagtacgga agcaacattg actgcccgtt
    43681 tgatcgaccg tccaattcgc ccgatgtttg cagaaggctt ccgtaatgaa gtccaagtaa
    43741 cgaacatcgt gatgagtgta gaaacagact gctcgccagc tatggcagca atgctaggtt
    43801 cttctttagc actatcaatc tcagatatcc catttgacgg cccaatcgca ggcgtagaag
    43861 ttggtcgtgt aaatggcgag tacgtcttga atccaacagt agaacaagca gaacaaacag
    43921 atatcgaatt gactgttgcc ggaacgaaac aagccatcaa tatggttgaa agcggcgcga
    43981 aagaagtttc agaagaagac atgcttggtg cattgctgtt tggttttgac gctatcaaag
    44041 agttagttgc tttccaagaa gaaatcgttc aagcagtagg gaaagaaaaa atggaagtga
    44101 ctttactgca agttgatgaa gtattgaaaa aagaaatctt tgatgcttct tacgctacaa
    44161 tgaaagcagc agtaatgact gaagaaaaac ttgctcgcga agataatatc gagcaagtga
    44221 agatcgatat ccgcgaagct tatgctgaga aatttgcagg tcatgaagat gaagatcact
    44281 tgttgaaaga agttaaacaa atcacagaag atcttgaaaa agatgtcgta cgtgaattga
    44341 tcaccatcga taagatccgt cctgacgggc gtaaattgga tgaaatccgt ccattgtcat
    44401 cagaggtcag cttattgcca cgtgtacatg gatcaggttt gttcacacga ggacaaacac
    44461 aagctttatc agcttgtaca ctcgcacctc taggagagca ccaaatcatt gatggtttag
    44521 gggtggaagt cagcaaacga ttcatccacc actacaattt ccctcaattt tctgttggtt
    44581 ctacaggacg tgcaggctct ccagggcgtc gggaaatcgg acatggcgca ttaggtgaac
    44641 gtgcgttagc acaagtaatc ccttcagaag aagaatttcc ttatacgatc cgtttagtag
    44701 cagaagtatt ggaatcaaat ggttcttctt cccaagcaag tatctgtgct ggcacattag
    44761 cattgatgga tgcaggtgta ccaatcaaag cgccagtcgc cggtattgcg atgggacttg
    44821 tttctgatgg tgaaaactat acgatcctaa ctgatatcca agggctggaa gatcatcttg
    44881 gcgatatgga ctttaaagtt gccggtacaa aagatggtat cactgcttta cagatggata
    44941 tcaagattca aggaatcaca gaacaaatcc taactgaagc attaactcaa gcaaaacaag
    45001 cacgtatgga aatcttagaa gaattgactt caacgattgc tgctccacgt gaagaattaa
    45061 gccaatatgc accaaaaatc gaaatgatcc agatcgaacc tgctaaaatc aaagatgtta
    45121 tcggtaaagg cggcgatacg atcaacggaa tcatcgatga aactggtgtg aaaatcgata
    45181 tcgatcaaga tggaaaagta agtattgctt cagctgatgc agaaatgatc aaaaaagcta
    45241 tcaagatcat cgaagatttg accaaagaag tcaaagttgg tgaagtttat ctagggaaag
    45301 ttgtccgtat cgaaaaattc ggtgcattcg tcaacttgat caaaggaaaa gatggacttg
    45361 tccatatttc ccaattagca aacgatcgtg tcaataaagt cgaagatgtt gttaaactag
    45421 gtgatgagat ccttgtgaaa gtaactgaaa tcgataaaca aggacgcgta aatctgtcaa
    45481 gaaaagcaat gttgaacgaa gatggttcaa cgaaaaatga agaaaaataa tttaattcat
    45541 aggaataatg tacttgtgat cgtgaaggtg acagggggac aataattatt cgaaaaaagg
    45601 ggactgtgac aaaagccgtg tcacagtcct ttttttgcga ataaacggtg ttcaaaagct
    45661 tgcatctgcg gtaataagtc cagccaagtt aaaaacatta ggagctgttt ttacttggct
    45721 gttcttatta cttgatgcag aacagctttt ttacaacctc tccaataaac gatgttcaat
    45781 cagctgtccc tcgatattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
    45841 atttctctaa tactcaggtc aaatcgctga tttcacaacc tctgattcac ggtgttccag
    45901 aagcagcttc tacggaaata agctgaacac ttttgttaca acttcttttt ttctggagtt
    45961 tcttggcgca tatttttgga gtccttctga ataaagcgta aaataaaagt gagaaatatg
    46021 gataaaggag ggaaaggaga tgctgaattt tttatggtcg ttatttatcg gcggtatatt
    46081 aggtttgata gctggcgcaa ttttaggaaa ggaagtcccg ggaggcgtat ctggaaatat
    46141 cattatcggt tttttaggta gttggctcgg cgaattttta ctaggaccac tgggaccgga
    46201 actcggcggt ttttatttga taccagcatt attgggtgca attttttgcc ttgctcttta
    46261 ttcatttgtg gcaagacgta tcagagaacg tccaaagaaa gaataatttt attcaaataa
    46321 gttgacactt cagtgtgtta cttggataat tagaagcaat ttaggaaagc gggtgtcatg
    46381 gataagaata tcatctatca ttcaagctta cctaaaggat atccatataa caagaaatga
    46441 gggaaaaacg tggcaacaac gcacaatacc aaaaagatat caatggctgg cctgcttgta
    46501 gcgatgggag tcgtctacgg agacatcggg acaagtccgc tttacgttat gaaatcgatt
    46561 gttgaagata atggcggatt gtcaggaatc aatcctgatt ttgttatcgg atcagtctca
    46621 ttgatttttt ggacattgac tctactgaca acaatcaaat atgtcgtgat tgcattgaat
    46681 gcagataacc atggtgaagg tggaattttc tcactctaca cacttgtccg aaaaggtggg
    46741 aaatatttga tcattcctgc aatgatcgga ggagctgcct tactagcaga tggggtattg
    46801 acccctgctg tgacggtaac gactgcgatt gaaggcttgc ggggaattcc gcagtttttt
    46861 gaccggttcg gtaataacca gaacgtgatc gtcattatca ctttagttat tatttttgtt
    46921 ttattcatgg tccaacgctt tggtacagat attgtcggaa aagcattcgg accaatcatg
    46981 ttcgcttggt ttactttttt aggcgtaatg ggattaatga acttcagtca agactggaca
    47041 gttatccgtg cactgaatcc ttattatgct atccagctgc tttttagtgc agataacaaa
    47101 ttaggcttgt tcattcttgg aaatattttc ctggctacga caggtgcaga ggcgctttat
    47161 tccgatcttg gacatgtcgg caaacgaaat attcgtctga gctggcctta cgtcaaaatt
    47221 tgtttggtta tcaattattt tgggcaggct gcgtggttgt tgaatgttta tcaaaaccct
    47281 gaagcacagc aaatcaagaa cctgaatcca ttcttccaaa tgatgcctca aagttggatc
    47341 gtgatcggcg ttgcttttgc aacagtcgct gcagtcattg cttcgcaagc attgattaca
    47401 ggttcgttta cgctagtttc agaagcaatc aagttgaaac tgctgcctcg gttaaagatc
    47461 atgtatcctg ggaacagtat cgggcaaatg tatatcccag cagtcaatct gattttatgg
    47521 atcgcctgtt ctctagtggt tgtcacattc cgaacgtctc atcatatgga agctgcttac
    47581 ggtctatcga tcactgtgac gatgttgatg acaacagcac tgctttactt ttatcttatc
    47641 caaaatgggc attctcgttg gcttgcatat ttagttacct ttttcttcgg tgcgatagaa
    47701 gtcgttttct ttatctcaag tgttgtcaaa ttcttccgag gcggttttgt agccgttctg
    47761 attggtttga tcatcttagt cgtgatgttt atttgggaac aagggaacat tattcgcgaa
    47821 tcgtcaaccg aggatgtgtc tttgagggat tatatcccgc agcttagaga attacgtgat
    47881 gatacttctt taccaatcta tcagacaaat gtggtcttct tggttcctga tatggctgaa
    47941 gataaagttg gccgacagtt catctattcg attttagata aacggcctaa aagagcacga
    48001 gtctattggt ttgttcatgt ggaagtgacg gatgagccat atacgaaaga atatcaagta
    48061 gatatgatgg acactgattt tattgtccaa gtgaatctgt tcttaggttt cagggtccaa
    48121 caggaaatca acgtatatgt tcgccaagtc atccatgatt tgatgaaaca aggccgtttg
    48181 ccaaaacagc cacaaagata ctctctgaca ccagggcgag aagttggcga tttccaattt
    48241 gtcctgattg atgaagtagt ttccaatgtg acaactctag gaaaatggca acgtcaaatc
    48301 atgcaggcaa aactggcaat caaaaaaatc gctacctcgc cagaaacatg gtttgggcta
    48361 gagtacagcg aagtaaaaca tgaaactgtt ccgcttatca ttggaaatac tcggaaaaca
    48421 tggttgaaag agcggttgtg aaagtagcgc tttgacctga actaatgtcg aatgtcagct
    48481 acttgaacac cgtttatcag aggtcatgaa aaagctgtac tgcaacaagc agtaagaacg
    48541 agcagataaa aacagcttct catgtttttc atctgtttgg gcttaatgcc gcagatgcaa
    48601 gcttttgaat accgttttta tcagaggtca tgaaaaagct gtttagcaac aagtaacgca
    48661 atgattttga agtgataagg agatgtggct gaaaaatgcc gcatctcctt ttttattcct
    48721 aaatatgaat acgcattatt ttcaatcttt ttgtagaaaa agggtacaat agaaaaagag
    48781 aaaggcggga gaatatggtt atcaatttca ttattttatt tttgatgatt gcaattactt
    48841 cattttttgt agcaagtgaa tttgctttag tgaaaatcag acgatcaaga ttggaacaac
    48901 tagaaaagga aaatgtaaag aacgccaagc tagctcttca tgttacacac catttagata
    48961 actatttatc agctagtcaa ttaggaatta cgctaaccgg tttgatcatc gggtgggtcg
    49021 gtgaaggatc tgtcgcggct ttactagaac cagtgatcgg taaactacct tttagttcgg
    49081 ctattagcag tacaatctct gtggcgctgg gatttatttt agttacgtat atcgatgtgg
    49141 ttattggcga actgcttcct aagagctata gtatcgtcaa tacggagaag gtcgtcttgt
    49201 tcgtagttaa accacttcat tatttttata aagtaatgtt tccttttatt tgggtattga
    49261 atcattcagc tgcagggcta gggaagctat taggtgtgcg tctcgtatct gaaggagaag
    49321 agacattgtc tcaagaagag ctgacattag tcgcattgaa ttcttatgaa aaaggagagt
    49381 tgaccaaaga agaatatcat tatttggaaa acgtgtttga atttgatgat accttggcaa
    49441 aagatataca agtagaccgt acgtccatgc aagtattcga agcttcagaa accgtcaaag
    49501 aagcgataaa agcttcgatt gaacaaggac atactcgcta tccggttatc ttagaatcaa
    49561 aagacaatgt tttaggttat gtcaccttgc cagatttgat caagcaatct tataaagatg
    49621 accagctgac agtagaacag ttgattgaag agccgatcgt aacaactgaa acgatcccta
    49681 taaaaaaatt attgacgatc atgcgaaaaa agggaaagca tattgctatc ttaaaagatg
    49741 aatacggagg gacaagcggc ttagtgacga ttgaagatat tttagaagaa atcgtcggcg
    49801 aaatccgaga tgagacagat ttggacgaag cattggtagc ggaacagtca gatggctctt
    49861 atatcatctc cggcaaacta acattagatg atttccagcg ttattttcat gtagagattc
    49921 cagagtttga agaaacgaat tttactacac ttgcgggctt tgcttctagc cgatataaag
    49981 aaataaaagc aggaacaatc attgagattg catcattccg tttcacggta ttagaatacc
    50041 agcatgcaca tatcgattat ttcaaagtag agtccacgga aagaaaaaca gaataaaaga
    50101 aaaaaggctg tgacattttt tgtcacagcc tttttgttat ctaaaaaacc tgttttcggt
    50161 aaacgaagaa gatattttca ataaaagttt tcatttttag ccgcaacgat ttcttttaat
    50221 caaggctatc ttgcttctgc atgttctaat gtgaaatgtt cctcttcacc catgtgtttc
    50281 aattgtttca tcgaacgcat ataagagtaa agcaacacgg caacagaacc gatccaagct
    50341 aaaggagaag ctgctgctgc tcctgggtat ccccacattc ctgtaagaac gatagctgca
    50401 aaagaacgca taagcaattc catcataccg gcgatcgtag ggatcttgct ttgtccaagc
    50461 ccttgaagtg tgtagcgtaa aatgaacagg actgctaaga tccagtaaag agacccattg
    50521 atattgaagt aggtttgtgc taattcaaat acacgtgttt ctgatgggct aacaaacaat
    50581 tgaacaaaga aatgaccgaa gaaaatgacg atagcacctg ctaagagact aaatccgaca
    50641 ctcatcagta aagtttgacg taccccttgc aagattcggc catattcttt cgcaccatag
    50701 ttttgtgcag tgaaagttgc cattgcaata ccaaatgaca tcatcggctg atttgcaaat
    50761 tggtcgatac gaccagcagc ggcttgagca gctacgacat ctgtccctag actatttagt
    50821 gctgcttgta acacgatagc accaacagcg atgatcgaag attgaaacgc cattggcaaa
    50881 gcagcattta ggtgtacagc aaactcttct ttttcaaatt tgaaatcttt tttgcgtaac
    50941 tgtaacaaag gcattttttt cttgatatac actagacaca gtacgctgga agaaacttgt
    51001 gcgattactg tagcaatagc tgcaccttcc acacccatgc caaaatagat aataaagatc
    51061 aaatcaagta cgacgttgat gatgaccgca atgatcagaa agaatagtgg tgtgcggctg
    51121 tcaccaagtg cgcgaatgac attagaaagc aaattgaaac tgactgatgc aaagatccct
    51181 aataaaataa ttgaaataaa tgtttgtgct tgatcaagta tttcaggagg agtttgcatc
    51241 aattgaagca tcggacgaat aaacaggaga cttaggacag ttaaaatcac agtgacgaca
    51301 agactaatga caacactagc agcaaaactt ttcttcagac cccgataatc ttttgcaccg
    51361 taacgttgtg cggtaataat ggctaaacca gctgttaaac cttgggcaaa gccaatgatc
    51421 aaaaatgtca ggcttccagt tgcaccaacc gctgccagcg catttttacc tagcgtctga
    51481 cccacgatga tcatatcgat catattgtag aactgctgaa aaacatttcc gatcagtaaa
    51541 ggaacagtga acatcaaaat cagtttagcg gggcttcctt ttgttaaatc gcgcatcttt
    51601 taaactcctt atatctttaa aatttagtta gtagtatata gcgtttatcc tgagaatacc
    51661 aattagaaat caatgataat ttatgaaact ccataaaaag aagccaagaa ttgagtcaaa
    51721 gaatcaacaa cttggcctgg tctgtaaaat aagggacatc cacgtttatc tatcacctta
    51781 atattgtctg atctcacttg gtaaaaggat cgtttctctc gtaccgtcat cataggcgta
    51841 tacttgagca ggaaattggt tcgaataggc aaacggcaaa tcaatatcca tttccacatt
    51901 gcttcctgct tgagaaaaat gcatcgtcac ttttccacga ttttcgatca aatcaaaggt
    51961 taatatattt tctaatggaa tgactccctt aagatctagg tcaataataa accatatact
    52021 gtcaatcaat tcatctggca aacttgatac gacaccaact gaagcgtaac ggttgcggct
    52081 gctatcaaaa ctttcaaaca tctgtttaac ctccttcgaa tcaacgaatg ttttattttt
    52141 ataattaatc ctaggataaa ctagaaatac ttacttcaac gagggttcat tctaataaag
    52201 aatcaggaaa aatgcaacaa taattgaata ctttttttca aagtataaat ttttataaag
    52261 gtacgataaa agtatgcgag gaaaagcttg gtaaattagg atttttgcgt ttgcaaagct
    52321 atttgtaaaa gtggaagcct catatgaaaa taaacgatta gaaagaaaaa aaggcgtgtt
    52381 aaaagctgct gacccgccgc ttctaacacc acctgattgt ttcagtatta ttcttctgtt
    52441 gaaccgatta cttctggttc gccaccttca cctgtgcttt cttcttcttc actttgagcg
    52501 actacagcag cgatttgctc ttcactgtcg gtcacgaatt tgtaatcgtc gttttttgga
    52561 agatcggcta ctgtaataga atcacctaat tccaagtttg tcacgtcaac ttcaattcgt
    52621 tcaggcaatt tgtctggtgt agcagatacg actactgtat acaagttttg agctaacact
    52681 ccaccagatt tagcacccac tgattcgcca acaagaacga cttctgtttc gacttctgtt
    52741 tcttccgaca tgtttacaga tagaaattct gcatgttcga aatgattggt aaaagtgttc
    52801 gtttggatct tgtgaaccaa tgtattgact ttttgtccat cgatgtccat cgtgataact
    52861 gtgttcgctc catgttcacg caacaatttg cttagtgtca aaccatcgaa tgagataggg
    52921 gtactttcta ctttataccc ataaacaaca gctggaactt tcccttcgtg acgaagtttg
    52981 tttcttaatg aacggggacg tacttctctt ttacttactt ctaatgatac tgacataacc
    53041 aaaattcctc cttaaaatgt gactgtctgc tttggtaacg gacagatttt atttaagtga
    53101 attctaaagg ctaatctcgt gcacatcatc gaaggcaggg cgcttaaaaa agcgcaaaag
    53161 gagagatgcg cgcatctccc cttagaaagt caggattcaa cttttctcca ccaggtcatt
    53221 acgctgtgta tgagaaatcc aattcacttt actatttaac cgtaacgtga actgttaatt
    53281 aagtcaagga ataactatga tgatgtattt gcaagcaaga atattcaagg tataattaat
    53341 tagtagtaat aagaaaggaa acagcatgcg tttagataaa ttaatagaag aaaaattaga
    53401 aacgacacgc aaggaaatga agcggctgtt tgctttgaag caagtgatga tagatggcac
    53461 agtcgaattt cgacaaaatc gaaatgtaga tagtttactg catcaaatcg aagtagcagg
    53521 tgaacactta gaaacaaatg agtcctatta tcttctcaat aaacctgaaa aagctgttac
    53581 agctgtatca gatccagaaa agacgacagt aatcgatctg attgcaagtg ccgatcgttt
    53641 ccaaccatta tatccagtgg gaagactaga tagagataca acaggactat tgctgatcac
    53701 ttcgaatgga caattgggat atgaaatgct acttccagaa aaaaaggttt caaagactta
    53761 tcaagtagtt gtgaacgaaa gggtgacatc agcagacgta acagcatttg aaaatggaat
    53821 cgtattcgct gatgggtatc aatgtaagcc ggctgattta aaaatactca aagcagacaa
    53881 gcaccaaagt acggttttat tgacgatcca agaaggaaaa ttccatcaag tcaaaaagat
    53941 gttcttagcc caaggaaaga aagtgaccgc attgaaacgg ctaagcatgg gaccattgga
    54001 attagatgaa cagctaggag taggcgagta tcgatcgttg acattagcag agatgaaaaa
    54061 gttaagtatt tattttaaat aggaggtcaa aaagatgaaa tataaaactt tattgttcga
    54121 tgtagatgat acattgttag attttcaatt aactgaagcc aatgctttaa gtgctttgtt
    54181 tgcagaacaa ggaatcacat tgacaccaga aatcgaaacc agctataagg ctttgaatca
    54241 tcatttatgg caggagtttg aaaaaggcag actatcacga gaagaaataa taggcagtcg
    54301 ttttgggtta cttttccagc aatttgggaa aacagtagat agtgatcaaa tggaaaaacg
    54361 ctaccgccac tacttgaacc aaggtcatga tcttgttcca ggaagtctag aaatcctgaa
    54421 aaaggtccat ccgcatgctg aactttatat tgtgacgaac ggtgtgtcgc acacgcaaca
    54481 tcggcgatta acagatgcag gaatgcttga ttacttcaag gatatctttg tctcagaagc
    54541 tgtcggagcg caaaagccga tgaaagaatt ttttgatcat gtcttttccc acattcctaa
    54601 ttttgataaa aagcaaactg tgatcattgg tgattctttg acttctgata tcaaaggagg
    54661 aaatatggca gggatcgata cgatttggtt caatagaaat aaattaccag aaatcgaaga
    54721 aatccagcct acttatcgaa tcgattcatt agaggaactt tattcattat tggaaattga
    54781 aaaggattaa ttttcagaaa aatatagcat tttcgtgttt tttcgctata atagattaga
    54841 atgaaaaaac ggatacgaaa gaggttttag tcaatgacaa aaagcaagcc aattatcatt
    54901 ggtgtgacgg gtggatccgg aagcggaaag acaagtgtca gccgtgcgat tttcaacaac
    54961 ttcccggacc attccatcat gatgcttgaa caagattctt attacaagga tcagagccat
    55021 ttgagttttg aagaacgatt gaacacaaat tacgatcacc catttgcctt tgataatgat
    55081 ctgctgattc agcatgtagg cgacttactg aactataaag ccattgaaaa acccgtgtat
    55141 gactatgtag cgcatacaag aagtcaggca acgatcattc aggaaccaaa agaagtgatc
    55201 attctagaag gaatcctgat tttagaagac gaacgtctgc gtgacttgat ggatatcaaa
    55261 gtatacgtag atacagatga tgacattcgt atcattcgtc gtatcaaacg ggatatggaa
    55321 gaacggggac gcacattgga ctccgtgatc gaacaatatt tgacagttgt taaaccgatg
    55381 tatcatcaat ttatcgaacc aacgaagcgc tatgcggata tcattgttcc agaaggcgga
    55441 gaaaaccacg ttgctattga tttgattaca acaaaagtag ctagtttctt aaaccataaa
    55501 tagtgcgcag cgaaacagtt gaacggactg cgaccgtttg aaaagcaagg attctgcttt
    55561 ttgaacagtc accagccaac tgttttttta tttataacaa gaggttgtga aaacagcatc
    55621 ttgacctaag tattaaaaag aagaaacgga aaatagctgt ttatatttta ccgttttttg
    55681 agctaatacc gaaggacagc tgattgagca ccgtgaagta gaggttgtaa aaaagctgtc
    55741 tttgcatcaa gcgataagag catccaaata aaacagctcc tcacgttttt cgttgggttg
    55801 ggcttattat cgtagatgca agcttttgaa caccgtttaa cgagaggttg taacagaagt
    55861 ggtcagatcc aataaataag aagaaactta cgaaaattat ttctcgaatt tgtgtgagat
    55921 ttcgtcttat ttttttgaag aagctctttt gtcgcacccc cta
//
LOCUS       DAJSVF010000009        55304 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSVF010000009 DAJSVF010000000
VERSION     DAJSVF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.009"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            435..1565
                     /locus_tag="ON550_000516"
     CDS             435..1565
                     /locus_tag="ON550_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3953628.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1657..2412
                     /locus_tag="ON550_000517"
     CDS             1657..2412
                     /locus_tag="ON550_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3953629.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2470..4509
                     /locus_tag="ON550_000518"
     CDS             2470..4509
                     /locus_tag="ON550_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR3953630.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4618..5769)
                     /locus_tag="ON550_000519"
     CDS             complement(4618..5769)
                     /locus_tag="ON550_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR3953631.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5923..6453)
                     /locus_tag="ON550_000520"
     CDS             complement(5923..6453)
                     /locus_tag="ON550_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3953632.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6552..6902)
                     /locus_tag="ON550_000521"
     CDS             complement(6552..6902)
                     /locus_tag="ON550_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR3953633.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7059..8588)
                     /gene="ahpF"
                     /locus_tag="ON550_000522"
     CDS             complement(7059..8588)
                     /gene="ahpF"
                     /locus_tag="ON550_000522"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR3953634.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8598..9161)
                     /gene="ahpC"
                     /locus_tag="ON550_000523"
     CDS             complement(8598..9161)
                     /gene="ahpC"
                     /locus_tag="ON550_000523"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR3953635.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9305..9967)
                     /locus_tag="ON550_000524"
     CDS             complement(9305..9967)
                     /locus_tag="ON550_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3953636.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10151..11797
                     /locus_tag="ON550_000525"
     CDS             10151..11797
                     /locus_tag="ON550_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3953637.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11788..12870
                     /gene="uxuA"
                     /locus_tag="ON550_000526"
     CDS             11788..12870
                     /gene="uxuA"
                     /locus_tag="ON550_000526"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3953638.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12978..14531)
                     /locus_tag="ON550_000527"
                     /pseudo
     CDS             complement(12978..14531)
                     /locus_tag="ON550_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            14679..15820
                     /locus_tag="ON550_000528"
                     /pseudo
     CDS             14679..15820
                     /locus_tag="ON550_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15946..17025)
                     /locus_tag="ON550_000529"
     CDS             complement(15946..17025)
                     /locus_tag="ON550_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3953639.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(17128..17568)
                     /locus_tag="ON550_000530"
     CDS             complement(17128..17568)
                     /locus_tag="ON550_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3953640.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17625..18035)
                     /locus_tag="ON550_000531"
     CDS             complement(17625..18035)
                     /locus_tag="ON550_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3953641.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(18215..18649)
                     /locus_tag="ON550_000532"
     CDS             complement(18215..18649)
                     /locus_tag="ON550_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3953642.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(18749..19597)
                     /locus_tag="ON550_000533"
     CDS             complement(18749..19597)
                     /locus_tag="ON550_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3953643.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            19986..21752
                     /locus_tag="ON550_000534"
     CDS             19986..21752
                     /locus_tag="ON550_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR3953644.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21854..23113
                     /locus_tag="ON550_000535"
     CDS             21854..23113
                     /locus_tag="ON550_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3953645.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            23260..24561
                     /locus_tag="ON550_000536"
     CDS             23260..24561
                     /locus_tag="ON550_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3953646.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24564..25415
                     /locus_tag="ON550_000537"
     CDS             24564..25415
                     /locus_tag="ON550_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3953647.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            25428..26243
                     /locus_tag="ON550_000538"
     CDS             25428..26243
                     /locus_tag="ON550_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR3953648.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            26394..27560
                     /locus_tag="ON550_000539"
     CDS             26394..27560
                     /locus_tag="ON550_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3953649.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            27790..28437
                     /locus_tag="ON550_000540"
     CDS             27790..28437
                     /locus_tag="ON550_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3953650.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            28521..29465
                     /locus_tag="ON550_000541"
     CDS             28521..29465
                     /locus_tag="ON550_000541"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR3953651.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            29600..31051
                     /locus_tag="ON550_000542"
     CDS             29600..31051
                     /locus_tag="ON550_000542"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR3953652.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            31072..31908
                     /locus_tag="ON550_000543"
     CDS             31072..31908
                     /locus_tag="ON550_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3953653.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            31908..32567
                     /locus_tag="ON550_000544"
     CDS             31908..32567
                     /locus_tag="ON550_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3953654.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            32693..33382
                     /locus_tag="ON550_000545"
     CDS             32693..33382
                     /locus_tag="ON550_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953655.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            33388..34344
                     /locus_tag="ON550_000546"
     CDS             33388..34344
                     /locus_tag="ON550_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR3953656.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            34388..34900
                     /locus_tag="ON550_000547"
     CDS             34388..34900
                     /locus_tag="ON550_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953657.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(35156..35704)
                     /locus_tag="ON550_000548"
     CDS             complement(35156..35704)
                     /locus_tag="ON550_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3953658.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(35793..36974)
                     /locus_tag="ON550_000549"
     CDS             complement(35793..36974)
                     /locus_tag="ON550_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR3953659.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(37210..38718)
                     /locus_tag="ON550_000550"
     CDS             complement(37210..38718)
                     /locus_tag="ON550_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR3953660.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            38877..39068
                     /locus_tag="ON550_000551"
     CDS             38877..39068
                     /locus_tag="ON550_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953661.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            39271..40188
                     /locus_tag="ON550_000552"
     CDS             39271..40188
                     /locus_tag="ON550_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR3953662.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            40181..41254
                     /locus_tag="ON550_000553"
     CDS             40181..41254
                     /locus_tag="ON550_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953663.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            41402..41920
                     /locus_tag="ON550_000554"
     CDS             41402..41920
                     /locus_tag="ON550_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953664.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            41901..42293
                     /locus_tag="ON550_000555"
     CDS             41901..42293
                     /locus_tag="ON550_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR3953665.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            42369..43304
                     /locus_tag="ON550_000556"
     CDS             42369..43304
                     /locus_tag="ON550_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR3953666.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            43437..44216
                     /locus_tag="ON550_000557"
     CDS             43437..44216
                     /locus_tag="ON550_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3953667.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            44209..44526
                     /locus_tag="ON550_000558"
     CDS             44209..44526
                     /locus_tag="ON550_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3953668.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            44770..46380
                     /locus_tag="ON550_000559"
     CDS             44770..46380
                     /locus_tag="ON550_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3953669.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            46537..47385
                     /locus_tag="ON550_000560"
     CDS             46537..47385
                     /locus_tag="ON550_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3953670.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            47616..48095
                     /locus_tag="ON550_000561"
     CDS             47616..48095
                     /locus_tag="ON550_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953671.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            48128..50356
                     /locus_tag="ON550_000562"
     CDS             48128..50356
                     /locus_tag="ON550_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3953672.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            50485..52128
                     /locus_tag="ON550_000563"
     CDS             50485..52128
                     /locus_tag="ON550_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3953673.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(52201..52746)
                     /locus_tag="ON550_000564"
     CDS             complement(52201..52746)
                     /locus_tag="ON550_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR3953674.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(52769..53254)
                     /locus_tag="ON550_000565"
     CDS             complement(52769..53254)
                     /locus_tag="ON550_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3953675.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(53244..54056)
                     /locus_tag="ON550_000566"
     CDS             complement(53244..54056)
                     /locus_tag="ON550_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR3953676.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(54195..>55304)
                     /locus_tag="ON550_000567"
     CDS             complement(54195..>55304)
                     /locus_tag="ON550_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3953677.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        18318 a        10023 c        10705 g        16258 t
ORIGIN      
        1 cttatttgtt atttttttct aaaaaatatt aaagattgtt aaatttcact atttcatttc
       61 aaattccatt gcctaatcat taggaaaagt ctatcatgaa ctcgtaaata gaatatagta
      121 ataaccttgt taggtgaggc tcctatacaa acataggctg ttgcccagaa acgtcgaaag
      181 tcgccaatgg gtaaaacaga aactgttgga ttaaggcttt ttccaatagc taagaatgtt
      241 ttctttacgt tgtatagtgc taaaactcgg acgatgagat gatgctaggt cagaattctt
      301 gttcgaaaag gtttctgacc ttttctttat atttttgtga ctgacttatt tgatgagaca
      361 aactcgctcc tcacctgaca agtggattgc tgtgtgagga ggagatgcgg tgaaaaaaga
      421 tcgattggtg gattttgggt ccttatttga ctttgtccat gatcggtctt ttagaagtat
      481 acagtgccag ttcttatcgt ttattgcaag ccgatgaaaa tacaaaaagt ctattattgc
      541 gtcaactaat tttcatattt ttgagttgga gcgtgatctt cttagctcgt tcagtcaaac
      601 tacactattt acttcaccct aaaatagcag gatacggttt agccttatcg attttctttt
      661 tagtattagt aagaataggg atattcggtg tcactgtcaa cggcgcacaa cgttggatct
      721 ctctgtttgg cattcaattc cagccttctg aactggcaaa tctttttttg attttttatt
      781 taagctggtt ttttcgtgac ggaaatagta gcccaaaaga tctaaaaaaa ccattcctga
      841 ttacagtagg tataactttt ctgattttat ttcagccaaa gattgctgga gcattgatga
      901 tcctttcgat tgcgtgggtc atattttggg cagcggcggt tccatttaaa aaagggatct
      961 atctaatcgt tactttttct gcattgctga ttggagcagc aggcggggta ttatatttag
     1021 gaaataaagg ttggcttcca cagatgttta atcatgctta tgaaaggatc gcgactttaa
     1081 gagattcttt tatcgatagt catggtgcgg gctatcaaat gacccattcg ttttatgcac
     1141 tatataatgg tggcatattt gggcggggat taggaaatag tatcacaaaa aaagggtatc
     1201 ttccagagac agaaacagat tttatttttt cgattattac agaagagctt gggctaatcg
     1261 gtgcattgtg tgtgcttttc ttattgttct cattatgcat gagaatcttt tgtttaagca
     1321 gtcgctgtaa aaatcagcaa gctggattgt ttcttttagg attcggaact ttgctattcg
     1381 ttcagacgat aatgaacgtg ggaagtatcg cggggctgat gccaatgact ggtgtgccat
     1441 tgccatttgt tagctacgga ggaacaagtt atctgatcct ttctttagga atcggaatca
     1501 cgttgaatat ttcttcaaag atacaggccg aagagttggc gctctatcga cctgaaaaac
     1561 aataaagaga aactgagaaa ctctcagttt gaattatcat aaagtacaat cttttatttt
     1621 tcaagcgaga ggttctttta gaaagaggaa ttcgcaatga aagtatctca aaaaatcata
     1681 ttatctattt tgttactgct tacattagta gttggctatt tttcgattga gtttatccat
     1741 ggattttcgt ctgcaaaaca gagttcaact gtgaaaaagg tagagccgaa aagtatccct
     1801 accacactaa atgtggcttt gattggttca gatgctcgtt cgaaagacga aaacggtcgc
     1861 tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct
     1921 atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct
     1981 tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa
     2041 gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt
     2101 cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac
     2161 aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac
     2221 aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag
     2281 cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt
     2341 agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa
     2401 gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag
     2461 gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc
     2521 ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa
     2581 gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac
     2641 aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca
     2701 ttatctgaaa aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa
     2761 ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca
     2821 tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca
     2881 ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa
     2941 atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata
     3001 gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca
     3061 agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc
     3121 gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac
     3181 tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt
     3241 caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac
     3301 gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa
     3361 atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg
     3421 aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt
     3481 caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaac agcttttgac
     3541 agtctaggag gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt
     3601 gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa
     3661 gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt
     3721 tatgctcctg gatctacgtt taaaatgatt acagcagcaa tcggtctcga caacggcact
     3781 atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg
     3841 ggatcttatc aagcaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct
     3901 ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa
     3961 tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg
     4021 aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact
     4081 ggatatggac agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt
     4141 tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat
     4201 aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt
     4261 gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg
     4321 aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc
     4381 ttgtttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa
     4441 gaagatgatg attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat
     4501 tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc
     4561 cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca
     4621 cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa
     4681 taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc
     4741 aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa
     4801 tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt
     4861 caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa
     4921 atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc
     4981 ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa
     5041 aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca
     5101 aataactagc gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag
     5161 taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc
     5221 aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct
     5281 gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga
     5341 tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga
     5401 agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt
     5461 gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc
     5521 aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa
     5581 taagatcaca ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa
     5641 tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa
     5701 tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat
     5761 aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat
     5821 tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg
     5881 attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt
     5941 tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa
     6001 tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat
     6061 caacaacaga atactactat aaacactcca acttttagct tttgtattca tgctgaagaa
     6121 caactggaac aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac
     6181 gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa
     6241 cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa
     6301 caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga
     6361 agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta
     6421 tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt
     6481 aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat
     6541 ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg
     6601 atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt
     6661 acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca
     6721 aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa
     6781 cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct
     6841 tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc
     6901 ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga
     6961 gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc
     7021 cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg
     7081 aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga
     7141 ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg
     7201 gtcactattt catttcttgg attcatttcg atcgttccat ctagccaatc cgtattaggg
     7261 agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg
     7321 cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca
     7381 tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct
     7441 tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt
     7501 gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa
     7561 gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt
     7621 acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat
     7681 ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt
     7741 tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat
     7801 tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact
     7861 tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata
     7921 gctgcactac tagctgctgg tccaccgcca atcactaata catcataagg ttctttttca
     7981 aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg
     8041 cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta
     8101 tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt
     8161 aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta
     8221 acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca
     8281 ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca
     8341 tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa
     8401 aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct
     8461 atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa
     8521 aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg
     8581 tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg
     8641 ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca
     8701 gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc
     8761 atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc
     8821 tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg
     8881 tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta
     8941 tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt
     9001 aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata
     9061 ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg atgataagca
     9121 tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata
     9181 attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt
     9241 atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa
     9301 aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca
     9361 tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag
     9421 ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca
     9481 ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt
     9541 cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag
     9601 cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta
     9661 ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt
     9721 ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac
     9781 tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg
     9841 gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt
     9901 ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc
     9961 ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga
    10021 aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg
    10081 acatagcgat ttttattgca taaaattcac ttgaatacaa aaactaatat gttagtttag
    10141 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
    10201 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
    10261 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
    10321 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
    10381 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
    10441 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
    10501 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
    10561 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
    10621 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
    10681 tattcttgct gtgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
    10741 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
    10801 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
    10861 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
    10921 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
    10981 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
    11041 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
    11101 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
    11161 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
    11221 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
    11281 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
    11341 tccaaatatt cctaatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
    11401 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
    11461 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
    11521 aggaaaacca ttcatcccga gtccagaccc actattgaca gaccttcagc ataagctttc
    11581 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
    11641 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
    11701 agaaatgttc atcaagatgg tagcaggacc aaatgccgta cgaaaaacat tacaggatta
    11761 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
    11821 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
    11881 acattgatgg atataccagt aggagaggta tggcctaggg agaggatcaa agaactaaaa
    11941 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
    12001 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
    12061 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
    12121 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
    12181 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
    12241 gaatttggat tgccgggatg ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa
    12301 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
    12361 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
    12421 tactcgattt ttggattagc tcgaatcgta aagaatcgag aagatttaga ttggttgtgt
    12481 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
    12541 ccagaaaatg atgtatatgg gattttagca gaattttgcc aaagagatcg aatcccattt
    12601 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accacataaa
    12661 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt
    12721 aacggataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
    12781 tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc
    12841 gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat
    12901 aagaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt
    12961 tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt
    13021 gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct
    13081 tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc
    13141 aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc
    13201 gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca
    13261 cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt
    13321 actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt
    13381 tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaat
    13441 acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta
    13501 agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt
    13561 tcaggtataa acaaatcaaa tacagtacaa agggactcca ccatttctct tgaataaaga
    13621 acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat
    13681 tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa
    13741 attggcagat taggtcgcaa aatatctaca gagagatcaa taaaaaagtc aatatattca
    13801 tactctgtga attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat
    13861 atttttaata gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct
    13921 tttttaaaaa aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct
    13981 gtagcagtac ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc
    14041 tcttttccag aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtactat
    14101 gctaaaaatg cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt
    14161 tgctgactga aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga
    14221 taactgactg attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca
    14281 attccttcaa aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga
    14341 ttgagacgct caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac
    14401 atatggaaat gatctgctaa atattgtgta gtaataaaga aacgatgttc agataggaaa
    14461 agaaaaatat ctaattttgt attgcttttt ttcttcaaga taaacgtttt aatcgcttct
    14521 tgagtttgca tgaaagaggc tcctttttac ttaaattgtt aaaacttgaa aatattactg
    14581 tttttttcat aaatttagac aaaaaaacag taaacatata ggtagaatct ctactatctt
    14641 atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca accatcttaa
    14701 tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc cacacagagg
    14761 agacagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga attacctttc
    14821 aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga caaacagcga
    14881 ccatcaaagc aaaaattgac tattcgggag acttagcacc tatactacaa tcagctgtta
    14941 catttgagaa tgttgatccc actttgtcga ttggctatga cgagaacact ttgactatcc
    15001 aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg ttaaaaccag
    15061 cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcattta acgccgtata
    15121 gtcagcggca gacaattgag caagattcag ccatcgactc aggaggagat cttgttgaag
    15181 aaccaacaga taaaccagaa aatgaaaata agccagaagt accgcctaca gaaaatccag
    15241 acggagaaca gaaaccggaa atagaacctg gagaagaacc cgatacagaa actcaaccag
    15301 agccagacaa taaatcaaaa cctgaaataa cccctggaga aaaaccagat gtaactccag
    15361 aaccagatac ggattctgga aaccaaacag taccggaaac aaatccagac acagataatg
    15421 aaactgaaaa tccagaaaaa ccagaggtag atccagaaga aaaaccagat gtgactccag
    15481 aaccagatac ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa aaaacaattc
    15541 aagaggatgg aaaaaaagag agtaaaaaat caaatctagc aattctaaaa ataaatgaag
    15601 agcagctgaa taaaaaatca cgaatatttg atagcgccca atcagctgaa accttaaaaa
    15661 gtagcaagga tacaaccttt gctgtcctga aacgaaaaat aaacagctac caaaatccgg
    15721 agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta tagcaacaat
    15781 gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata atgtctgtga
    15841 acgcattttt caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc agattgaaat
    15901 ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag ggatatctgt
    15961 ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa agaaggtaaa
    16021 gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa caagtaagac
    16081 cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg gattcagcac
    16141 ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa catctcggat
    16201 gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa cacctgctac
    16261 tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat gaaagccaaa
    16321 tacagccaag gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa attgagcttc
    16381 taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat cttgaaagaa
    16441 ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt tcttttcaat
    16501 cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt ttcctatatc
    16561 ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa aatcgtttcg
    16621 ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct gatgctcgta
    16681 aaaattaata acggactgga tcatgtcaaa tgtttgatga ataagcatcg ggatatactt
    16741 tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag ctaaaacacc
    16801 tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca aataagtaaa
    16861 gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa caaaaacgac
    16921 aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct gataaacggt
    16981 caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat gatggaatat
    17041 gaaaataaga aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg agttggtttt
    17101 cctcataaaa tcgttcatct agcagtttca gccctttttc tggtcttgat gaaaattatc
    17161 tagcattttt cgggtcagat gttgcagtgt atccaattct tcctgttcaa gtggaagata
    17221 agacttgatc ttttgtggaa taccttctgc ttgtttttgc aacgcagcac cttgagcagt
    17281 cagcgtaaca tttacgattc tttcatcacc cgtttcacgc tgacgattaa tataattttt
    17341 ttctgctaac ttttttagta agggagtcag cgtaccagaa tccagacaca aatcatttcc
    17401 tagctctttc actgaagtct ttttcttttc ccataaaact aacatcacaa gatactgggt
    17461 ataggtaaga ttcagcggct tgaggagggg agtgtagtgt ctaatgattt ctttagaggc
    17521 ggcatataaa ggaaaacata cttgattaac taaatataga tcttccattt ttccacctcc
    17581 gctgttttac tatagtgtat catagcggat ttacacaaaa aagtctattc actaactgct
    17641 ttcagagtaa cttctatatt tccagctgta gcttttgaat acggacacac tttatgcgct
    17701 gcctctaata attcttgcgt ttcttccaga gaaagacctt cgatagatcc ttcaatctct
    17761 acacctaaga caaagctcgt ttcactttct gaataaaggg aaacttgtgc actgacagta
    17821 gaagccccag agatattttt ttgctgtttg atcaattcta atgcactgtt aaaacatgca
    17881 ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg tttaccagga
    17941 gctgcaatat tcaattcgaa tgtacgatcg ggattatgca cttctccaga gcgtccgcct
    18001 gtgttgatca ttttcgtaga atacattttt ttcataaaat taccaccttt aattatattt
    18061 tatacaattg aattttacac aatataattg tggaaagcaa agaaatggat tggcataaga
    18121 taaataagag aaagcacatg aaacagacgc gctttctcga atagcaaaca tatacaaaaa
    18181 atatcagaga caaaaaataa tctcatcata aacattacat atatttctca aaaagttgtt
    18241 ttctttcttc aaaaattact tcacgatgga cctgatgaag catccataaa taaccaaaag
    18301 gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct
    18361 ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat
    18421 tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt
    18481 catccagcat atggaatctc gtaccataca aggtaaaaat gacttcatta ttgcctctag
    18541 ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg
    18601 ctttcaaact atcatttaat acaaaatcga tttccgttcc tttatgcata aagatctcct
    18661 ctccttttca gtcgaacagt tactagattc ttttattata ccagctaaag aaaacgcatt
    18721 caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc
    18781 ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca
    18841 atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact
    18901 ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta
    18961 agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat
    19021 tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat
    19081 aaagaaactc ttctattcca aaaataaaca gattaagaga aacaacgaat gcgtctgtaa
    19141 aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg
    19201 tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg
    19261 cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg
    19321 caatgattaa aggaaaaacc aagcttccga aaagtacttt aaaaaagggt tttttaccta
    19381 aaaatagaag agctagtacc aacataacga tattcaacac taaaataact atagctcggt
    19441 caaagcctaa agcagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat
    19501 gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct
    19561 taatgtatgt ccatataaat tgtctcgttt ctttcattta ttcacttcct taattcatta
    19621 ataatactct tatcttatta taacttactt ttcaccttaa gttaacttta attaataaaa
    19681 aaattaggct agtctaaatt attatgtttc attcttctag aatatacttc tacgccaaaa
    19741 actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag ataaattcag
    19801 gacactaaat agtacaggaa gtagactaaa acttttaaaa gttttaagtc tatctatttg
    19861 gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca tttttgaaag
    19921 cgttgtcttt tgcttctcta tcacttatag tgatgacgtc atattaaaac acaaggagga
    19981 aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca tttctatata
    20041 cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg
    20101 tgctatgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa
    20161 tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg acgagcgaaa
    20221 caagtcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac tgtttttacg
    20281 gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat ttttattttc
    20341 gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta aaagaaacag
    20401 tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat gaccccgaac
    20461 acactttacc ttggggaagt aaagatccag accgagaaga ctttttcggg ggagatttgc
    20521 aaggtgtgat ggatcatttg gattacttga cggaccttgg catcaatggc atctattttt
    20581 gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat tatgaggtgg
    20641 atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca cataaaagag
    20701 ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca ccacagtggc
    20761 aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac atccgttttt
    20821 tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat atcccatatg
    20881 atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca gaagtgcaaa
    20941 aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat ggatggcgtc
    21001 ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa gcagttacag
    21061 aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa gcttggctgc
    21121 aaggggatga attccatgca gtgatgaatt atgcatttac agattcgatt aaagattatt
    21181 ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc
    21241 tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat gatactgcca
    21301 ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt
    21361 ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg actggtgaag
    21421 atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat ctagagctat
    21481 tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt atttctgaag
    21541 gttctactca gtggctaata gtaaatgatc gtgaagataa gctctatttc acacggaaac
    21601 tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg gtagtagaac
    21661 aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg aaagcagaga
    21721 tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca acaagaaagc
    21781 gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt tgaatacaaa
    21841 ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt taatctcttt
    21901 aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag aaggggaagc
    21961 cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt atataaacga
    22021 aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag aaaaggacat
    22081 gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac ctgacgttat
    22141 gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa
    22201 actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt atgatggaaa
    22261 aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca aagatttagt
    22321 cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca gtcgttttgc
    22381 ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg acttttatta
    22441 ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc
    22501 ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg caacagattg
    22561 gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag acttcgccag
    22621 ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc aagcgcaaac
    22681 atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa ataatggaca
    22741 gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa
    22801 agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag aaaaattctt
    22861 tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa
    22921 cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga tgccaaacat
    22981 cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg cagcatcagg
    23041 ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg aagccataga
    23101 acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa caggtgcttc
    23161 agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca ttatcttaaa
    23221 agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga taaaacaagc
    23281 aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg gcagtttgta
    23341 aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt cattggtcag
    23401 atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag tataccaatg
    23461 gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat tacaatcatt
    23521 ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa
    23581 gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg tgatgaaggt
    23641 tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat cattttccca
    23701 gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat tccaccagcc
    23761 agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt aagctcatat
    23821 cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg tgcaacaact
    23881 ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt tattaaattc
    23941 aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt tattactatc
    24001 atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca agtgattcct
    24061 ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac ggacccgaac
    24121 tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta tatctatgtg
    24181 atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc aaaaatcgac
    24241 ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt cctagtggca
    24301 gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat gatctatctg
    24361 tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga tattcttatt
    24421 tcctggatct ataaacttac gactggaact tctccgcagt attctatggc ttcagcaatc
    24481 acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa aaagacaaat
    24541 gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga ttccaaaaac
    24601 gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt atcatctatc
    24661 cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct tttagtttag
    24721 atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa acactttacg
    24781 ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta caagtgactg
    24841 tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc aaatcaagtt
    24901 tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact gccttttatg
    24961 tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg atctatatag
    25021 gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact gtaccaatag
    25081 atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt ttccagattg
    25141 tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt atgggacctt
    25201 ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag actgttgctg
    25261 tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt tttgctgctg
    25321 gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa aagaactttg
    25381 ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg caaacatttc
    25441 cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga cgcaaactat
    25501 tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg gtcatgcctg
    25561 tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt ctatccgttc
    25621 attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta tctgaaactg
    25681 ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta ggtgtgtctt
    25741 tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag gaacaatggg
    25801 tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat agcccatctt
    25861 tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa agagaatttt
    25921 atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt cttgtcctat
    25981 ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg acaagaaaaa
    26041 gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg aatgttatgg
    26101 gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt atcttgatgc
    26161 taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc aaaacaagat
    26221 tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa
    26281 aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt
    26341 cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa atcatgttag
    26401 agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc gaagtaaatt
    26461 attgggacgg tacaacgaaa caatacggag atacggacac tcctccccaa atcaaaatca
    26521 atatccatga agaaatccca atgaaagaga ttacaagtaa cgcatctttg gcactaggag
    26581 aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg atcaacgatg
    26641 cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg ctgccaaaga
    26701 aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat gacttaggta
    26761 acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct tatttcaata
    26821 cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc ttagacaaac
    26881 tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga acattgatgt
    26941 tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt gaagaacaat
    27001 acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc cgtgtattat
    27061 taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt
    27121 tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa gatttattga
    27181 aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt gcgacaaatg
    27241 cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact gaattagttg
    27301 ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt cgtgactacc
    27361 aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag
    27421 aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt gcagcttcat
    27481 ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga aacaatgaca
    27541 tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt taaaaagaag
    27601 ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga ttgtctctaa
    27661 agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta atttttaaac
    27721 atgacagaaa attttttctt cgttatactt gtaatagaat actattttat aacaaagggg
    27781 atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt gtttttagct
    27841 gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag tagcgaacag
    27901 cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc tagccagaca
    27961 aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc
    28021 gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt agatagtgat
    28081 tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga
    28141 attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga tcgagatgaa
    28201 caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat cacgattgag
    28261 gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga ttgggatttg
    28321 gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca
    28381 tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa
    28441 tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga caaaataact
    28501 tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat atctttgcca
    28561 atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta gatgaactgg
    28621 ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa cgaaaaggaa
    28681 atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg ggacttccaa
    28741 acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc
    28801 aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc
    28861 ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta tcttgtccca
    28921 atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa acactgaaag
    28981 aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat tttgattttg
    29041 ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat gtcaatgcta
    29101 tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta gtcatcaaac
    29161 caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct ttggcgaatg
    29221 ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca ggtgggattc
    29281 ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta cagattggta
    29341 ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa ttaacgcaga
    29401 ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta cgcaccattt
    29461 catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa aacactcgtc
    29521 tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg aaaataacag
    29581 aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa
    29641 agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga agcaaaatca
    29701 tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg agctattgat
    29761 tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa tgctcggtca
    29821 agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac accgagcttc
    29881 tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt ttggacaaac
    29941 caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg gcccagcaag
    30001 aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg ctgctgcagt
    30061 ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta ttagaatacc
    30121 cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc cagttggtcc
    30181 atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag tttctatgag
    30241 agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc catatcaagt
    30301 atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt
    30361 tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa aaaaagctgt
    30421 agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac tagatggtcg
    30481 taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa tgatccgctc
    30541 gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca tttcttggac
    30601 tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc tgcatgtatg
    30661 ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat tcctttcacc
    30721 aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg cgataagagc
    30781 acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg tttcttccat
    30841 gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga ctggacaacc
    30901 agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa tccaatttat
    30961 ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg aagcaaatca
    31021 tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag aatgaagaaa
    31081 aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg cggtacagct
    31141 gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga attagtagta
    31201 gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga attagttggt
    31261 tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat ctcctttaaa
    31321 gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg
    31381 aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga gactttcaaa
    31441 tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa
    31501 gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg tttagcggaa
    31561 aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta tttaagcgat
    31621 gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa aatggccgta
    31681 tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa
    31741 agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga tggcgtatta
    31801 aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt tcaaactgat
    31861 gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg tactttcctc
    31921 taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc ttggtataca
    31981 cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg cttttgacag
    32041 ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat ttatcgttct
    32101 ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta ccttatcaat
    32161 taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca tttattggtg
    32221 aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct ggtttagctt
    32281 ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct tttcgaatct
    32341 cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg ttagcagcaa
    32401 caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg acattcgatt
    32461 atttttctct ctatatttta gttgcactga tttattgggt catttgtatc gggatcgaaa
    32521 aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa atagaaatgg
    32581 tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc ctagtatttt
    32641 aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac
    32701 cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc aaggcggctt
    32761 ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc ttagctgctt
    32821 gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga caaaagcaga
    32881 ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg ttttaccaag
    32941 taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt tagtgtatcg
    33001 tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg cgaaaaaagc
    33061 agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca tggcttcttt
    33121 attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt ttccaacaga
    33181 tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt ctaacgaacg
    33241 atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt tgcaagaaga
    33301 agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga tccgtcggtt
    33361 gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg aaaacaattt
    33421 gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa aatccaacca
    33481 gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa
    33541 aagcaccatc gaatcgctgt gatccaaaat ccccaagtag gacaattcaa attattcgat
    33601 cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc acagatctta
    33661 acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt tttcgatgga
    33721 cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga ttcgaaattt
    33781 ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt
    33841 gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga tgactttttt
    33901 agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga tttcacgatc
    33961 gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat ccatctaacg
    34021 tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag taacgactct
    34081 gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa atggatcatg
    34141 cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc tctttaccag
    34201 caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca tcatttggaa
    34261 cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc aaaagttcag
    34321 gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat agaaaagagg
    34381 aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg tagaaatcat
    34441 taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa acgggcagaa
    34501 accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg tattaatagt
    34561 ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg tttacacaaa
    34621 aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc gtgtcgcttt
    34681 agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt tgttgacgat
    34741 tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta atgaaccgat
    34801 ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat ttccttttga
    34861 acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc atctgagcaa
    34921 agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca
    34981 gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca aatttttgtg
    35041 gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc gagaggtcgt
    35101 gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga tacatttata
    35161 tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg tttgacacga
    35221 ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct acataaaccg
    35281 ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc gattgcaatg
    35341 tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac tcatataaat
    35401 gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt ttctttttaa
    35461 tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct gcaaactcat
    35521 ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc tcttgatctt
    35581 tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa
    35641 tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca tagcaataat
    35701 tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa atatttccaa
    35761 agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt cacaatagca
    35821 cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt tgcgacaaga
    35881 tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat agaagagaag
    35941 ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt aaaatggaaa
    36001 ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt agtcgccatg
    36061 atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc aagaggggca
    36121 aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa gatagcattg
    36181 atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat cgccacatgg
    36241 aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt gttttcttca
    36301 atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga agccagaata
    36361 taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc gatcagtgcc
    36421 atataagatc cgacgatcga catagaaact gtagacatag ccgatacact catggtgaac
    36481 aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac ttccgattgt
    36541 cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc gttgatttta
    36601 cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata gcgtagaata
    36661 ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa aggtgcactt
    36721 cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga
    36781 tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt acgtaataat
    36841 acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa tttgattgct
    36901 tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc aataatacct
    36961 aataaataac tcaaataatc actcctattc caaattccgt tttattttac ctgttttgca
    37021 aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa cgcattcatt
    37081 ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa
    37141 gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt
    37201 aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc
    37261 gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc
    37321 ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac
    37381 tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg
    37441 aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat
    37501 gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc
    37561 catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac
    37621 agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg
    37681 gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg
    37741 attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg
    37801 acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc
    37861 tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt
    37921 ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac
    37981 tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt
    38041 ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt
    38101 ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa
    38161 ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt
    38221 tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac
    38281 tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt
    38341 gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa
    38401 taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc
    38461 ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc
    38521 agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag
    38581 tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat ataatttgtc
    38641 atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc
    38701 aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt
    38761 gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc
    38821 aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga
    38881 aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt
    38941 atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag
    39001 aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta
    39061 gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac
    39121 tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc
    39181 ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat taataagtga
    39241 aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat
    39301 gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga atcgtcttct
    39361 ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag
    39421 gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc
    39481 cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg
    39541 atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac
    39601 atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc
    39661 ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag
    39721 aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag
    39781 gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga
    39841 caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa
    39901 attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct
    39961 tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga
    40021 gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc
    40081 ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat
    40141 gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc
    40201 agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga
    40261 agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa
    40321 aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc
    40381 ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat
    40441 cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt
    40501 gatcgaatca gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga
    40561 accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa
    40621 accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag
    40681 acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt
    40741 accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga
    40801 gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt
    40861 ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt
    40921 agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc
    40981 acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt
    41041 tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca
    41101 agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt
    41161 cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga
    41221 attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc
    41281 attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc
    41341 ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg
    41401 aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag
    41461 gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt
    41521 actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag
    41581 tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct
    41641 gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg
    41701 agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct
    41761 gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat
    41821 gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta
    41881 caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca
    41941 gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg
    42001 aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt
    42061 cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt
    42121 ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga
    42181 aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag
    42241 agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat
    42301 aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg
    42361 aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg
    42421 ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca
    42481 tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg
    42541 gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa
    42601 aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg
    42661 tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg
    42721 ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga
    42781 aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc
    42841 gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct
    42901 tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca
    42961 gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt
    43021 atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt
    43081 ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg
    43141 agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg
    43201 tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat
    43261 acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa
    43321 ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg
    43381 aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga
    43441 acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa
    43501 gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc
    43561 tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat
    43621 ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat
    43681 tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt
    43741 ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt
    43801 tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga
    43861 ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg
    43921 ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga
    43981 aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg
    44041 tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat
    44101 ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag
    44161 atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta
    44221 tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg
    44281 atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta
    44341 ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg
    44401 gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa
    44461 acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg
    44521 ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc
    44581 tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa
    44641 agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg
    44701 tccttctatg attttttgct ataataggga agatgtttat tttatgaaag aatagttgag
    44761 ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt
    44821 ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat
    44881 caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc
    44941 ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca
    45001 aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt
    45061 gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc
    45121 agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg
    45181 attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg
    45241 ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt
    45301 acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca
    45361 gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga
    45421 aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac
    45481 cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc
    45541 attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt
    45601 ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat
    45661 aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata
    45721 ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt
    45781 tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca
    45841 ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg
    45901 ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat
    45961 caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa
    46021 gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc
    46081 ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta
    46141 gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta
    46201 tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca
    46261 atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc
    46321 ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag
    46381 gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc
    46441 gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat agttataagg
    46501 tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat
    46561 agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc
    46621 cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat
    46681 tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa
    46741 gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat
    46801 ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc
    46861 ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg acacatggta
    46921 gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc
    46981 gatcgaatcc gagataatac ctacgcttat ctgattgtag atgatttgaa caatttagta
    47041 cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg
    47101 atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa
    47161 gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc
    47221 aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg
    47281 cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca
    47341 catttaggtg aaaaagccat cggcattgcc atttcagcgc aataattcct tttattcaaa
    47401 aaacatacca aaaatattcc aacatcatct tggactttct acatgttact aatgagcgtg
    47461 taaaagaacg agatggtgtt tttatttcta tcaatgaagc tctttttctt tgcaatcctc
    47521 acttcaatcc tctataatgt cgcatatagt ttagtaaatg agtaaactac taatttactt
    47581 atttactaaa aataaaagga tgaggagtta aaataatgga aataccaaca aatttaaaac
    47641 ataaaccagt aatcgttgta gaagactatg ataaagttga tggaagaaat gcattaaata
    47701 cggatgcaaa agggctttca ctgggccttg ctcagtggaa tgatcgtgga aaagtcgata
    47761 tctcaggcaa ggtatggcga catactggag aaaaatggtc acgacagtcg gaagaattac
    47821 caatcacacg tattttagat ttagcgatct tagctgcaca aggaagttta tacttccaag
    47881 atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc agtcgtagac atcattggat
    47941 tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa tccaaagatc gatgaagaca
    48001 tcattttgta ttacgataca ctacaaaaag acggagaact gattggggaa cgattccgta
    48061 ttttaaaacg tcttttagat gagctaggat attaatagac agagatgatc aaaaggggaa
    48121 gagaagcatg gagaatttct cactaacaga aaaccgaacc ttttcacggc tagaaaagca
    48181 aatgatctgg aagaaaccga aaagccatcg gacaagtgca gaagaatggc ggatcgcctc
    48241 cgaaatcaaa gggaattggc aagatcctga aatgaaaatc agtaacgtac ttttggaagg
    48301 agatgccggg tcggggaaga cacaattagc caaagcacta tctgctgatc tgcaacttcc
    48361 ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca gatgttttcg gagccttatt
    48421 acctgtagta gattctaata aggaacaaga ccaagaatta ctagaagcta tttaccaaac
    48481 agatacactt gaagctgtac tgacactgat ccagaaccat tacggcattg atcctttttc
    48541 tgctaaagaa aaattagcag agctagttca aaggatcgaa gctgattatc cagatgcaat
    48601 ccattacagc tattaccctt ctgaaatcgt tcgagcgatt gaaaaaggat atttattaga
    48661 gattcaagag ccgactgtca tccgagatgc ctctgttctt gtggcactta actcagcgct
    48721 cgaaccaaat gggatgttga atttaccgac aggaatcatt cgtcgccatc cagattgcgt
    48781 cattgtgatc actacaaatc gaaattacca aggaaatcgc ccattaaatg aatctttaag
    48841 agatcggatg caacatgcgg aaaaaatgga tcttcctgcc ttagaagtca tggcagaacg
    48901 tgcgatggcg aaaactatga tccaaaaaca agaacttctc ttaaagatgg cagagattat
    48961 ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa ggtgtagcag gaatgcgttc
    49021 ttatttttat tgggcaaata cctataagca aggacaagat ttactccagt cgatttatcc
    49081 aaaagttttg tataaattat ccactgattc agatgaattg gcaattcttg aacaagcttt
    49141 gtctgacagt ggattgctac tggaacttca ggacatattg agaaaagaaa aatggggtca
    49201 tcctcaatct gagcatacaa aaggaagaac cattagcgcc gaagaagcaa atgaacgaaa
    49261 catcactgaa aatgctgaag aaattctgcg cttagatgaa aagaaatcgt tgtctgctga
    49321 agaaccaaat gagggagaag agaaacaaga agaaacaata gaaaaaaacg agattcctcc
    49381 tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa aatcaaaaag aacaacaaac
    49441 aggagatgat tctcaagaaa tgtctgcttc tgacatggaa gcacatgata aggaaatcaa
    49501 gaagcaactg aataaagaag cgcgacaaat catgaaggaa acgattcatg aaaaagaagg
    49561 tctcatcgtt caccgaccag ctttccaagg aaaaacgccg gaagcacgtg agctacacat
    49621 gcaagtaatg ccaatagtag aatcacttag ccggcaaatt ttagagttac tggataatga
    49681 acaaacagag acgtaccaaa aaggaaaata cgaaggtcag cggttcaatg caagcaaagt
    49741 agcatatggt gatttgagaa attttgacaa aaagaacccg ccacatgagc aaccttcttt
    49801 agcggtagct gtacggatcg atgaatctgg gtcaatgata cgggatgatc ggatcgaagc
    49861 ggcaaaaaaa gcagcgattg ccattgccga attttctaag aaagtcgata tcccattgtt
    49921 gatttacgga gatacagcag accgttcgtc aagagaaaag acttctttgt tttcttataa
    49981 agaatttgaa gacggatttc aatggttaaa tgaaaaatta gtcactatga aaccaaggca
    50041 aaacaatcgt gatggtgctg cacttcgttt agcagcagaa aaattaaaca gacaatccgc
    50101 aacaaccaaa ctgttgctta atattagtga cggtcagcca aaagcgttgc cggattatac
    50161 aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa gaatatgaac gtcaaggaat
    50221 cctatttgtc tccgcagcta ttggccaaga caaagaagaa atcaagtcga tctacggtga
    50281 aagtcgtttt gtagatatta ctgacttgaa tgaatttccc aagcaaatga ttcaactgat
    50341 tgcccgctat ctatgataaa ataaagctca acgaacgcac ttagcagatt cgttgagctt
    50401 ttcgtttcta gttaccggta acaaggatac ttctaaaata gaatcggtta taatcaagag
    50461 ggaccgttaa aggagtgaaa cgatatgata caagaacaaa aatggtggaa agaagcagta
    50521 ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg gcgatggtca aggagacatc
    50581 aatgggatcc gtgaaaaact taattatttg aaagatcttg gtatcggctt tatctggatc
    50641 aatccgatct atcaatctcc atttgtcgat aatggttatg acgtaagtga ctatcaagat
    50701 atagaagaga gattcggaac gatggaagat tttgatttat tgctaaaaga agcacatgaa
    50761 ttagggatca aagtgattat ggatcttgtc atcaaccata gttcggatca gcatcaatgg
    50821 tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg attattacat ttgggtcgac
    50881 ggggtagatg gcaaagaacc gaataattgg acatcgattt tcggcggttc tgcttgggag
    50941 tattctcatg aaactggaca gtattatctg catgtttttg caaaagaaca accagactta
    51001 aattgggaaa gcgagaaatt gaaagaagaa ctattcaata tgatccgatg gtggttggat
    51061 aaagggatag atggatttcg tttagatgcg atttctcatg taaaaaaaga tgaatattca
    51121 gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg tctcaggaat cgaagaacat
    51181 ttgaccgatt tgaaacatgt ttttgaagag tacgatatca tgactgtcgg agaagcaagt
    51241 ggtgtcactg cagaagaagg accacaatgg gtagggaaag acggttattt cgatatgata
    51301 ttcgaatttg atcatatcca tatctggcag caagaaaaag aagggcaatt agatgtcctt
    51361 aaattgaaac ttgcattaag cgcttggcaa acttcattgg atggtatcgg atggaacgct
    51421 ttatatatgg aaaaccatga tgttcctcgt gccgtttcag tgtttggaga tactcgtccc
    51481 gatttttggg ctatgtctgc aaaagcaatc gctatgatgt atttcttttt gcaaggtacg
    51541 ccatttattt atcaaggaca ggaaatcgga atgacgaata tgccctttga atcgatcgat
    51601 caagttgatg cagtagattc gaaaagactt tataaaaggc tgcttgcgga aggtaagaca
    51661 agagaagaag cattggatat cattcgtgaa acaacgagag acaacagcag gacacccatg
    51721 caatggactt cggaacaata tgctggtttt tctacacatg aaccttggct gatcactaat
    51781 ccaaatacca aaacgatcaa tgtagaacaa caagaatatg aaccagagtc ggtcctgcaa
    51841 ttctataaga acatgatccg cattagacaa accaataaag gtctgatata cggttcttat
    51901 aaagaatatc ttcatgaaca tccacaactt tatgtctatg aaagatattt agaagatgaa
    51961 caatatctta ttatggtaaa cttaacagaa tcacttgctg actatgaact tccaaaagaa
    52021 gcagatcagt cctggacatt actcttgtca aattcctcta gcggagaatt tgaagctaaa
    52081 ggcatactag caccttatga agctcggtta tataaaacaa acaagtaaaa cttcctcgtt
    52141 aaagttcatt agttggactt tgcgaggaag tttttcagat tgctctgtca attactcatt
    52201 tcaataattc gctctaggac caccgatata tttaggacga gagcgaaggg ctgtcacgtg
    52261 agcttgtcca agggttttca aacgaggtct gacaggtact tggacatgtt tgatatgcat
    52321 accgattgat gtgtcaccga tatcgatacc ggcttgtcca actacgtgtt ccacttcgac
    52381 aggatcattg aactggtcaa aagcagcaac agaacaagca cctccagcat gtagtgcagg
    52441 aacaacagag acaatttcga aattttttgc ttctgccact gttcgttcta ctactaatgc
    52501 tcggttcaaa tgttcacaac cttggacagc tagcgcaatt tctttaggat ccaacaactc
    52561 ttttaacgta cggatgatcc attgaccaac ttccgcactg gaattttttc caatatgtcc
    52621 tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga tcaccttttt tcaagtgtgc
    52681 ctcagccaat atgtcattaa ctatttcagt caattggtct tttaattctt tttctgacaa
    52741 cttcattttc taaaaccttc tttttcaatt aatattgctt ccgtgcttga atacgttgaa
    52801 gacttgcagt tataggaaag gcaagtccca ttccaatcag attttggaag atgtttcctg
    52861 gtatagaagc cactccagcc ggccaatcat ataaaatcca accggcgata ccatagccga
    52921 tgatcataac taagcttcct aataaaagcc ctaatcccca catacgagta gaagatttgc
    52981 tgaaagaacc agcaacatat ccttgtaacc cgtggatcac taatgaaaaa actgcccact
    53041 gcggatatcc tgaaatcaaa tcgatcaata gaccactgga agcaccaaca actaatccac
    53101 ccgttggacc ataaagaaaa gcagcggtat agatcccggc ctcgcataat gtcacaaatc
    53161 cattcgtagc cggtacaggg atcaaaattg ttaatgaaag ggcaactgtc aacgcaatca
    53221 gcatagcata gatcgttaat tgcttagttt tcatttctca ttcctccttt ttgaataccg
    53281 ccagaccaga cacttccggt atgatcgttc atcagcacgc cattagaaat acaatgggca
    53341 acaaattttt tactttctgc tactgctttg gtaaaatctg cccctaacac aagattcgct
    53401 gttattgcag aagaaaaggc acaaccggcc ccgtttactg ttgtagtgtc gattttttta
    53461 tgagaaaata cctcataatg atcaattgaa tagagaagat ccacagcttc atctccagcc
    53521 atccttgtgc cgcctttgat cacaatgttt tttgttcctg aagcagtgag ttctttagcg
    53581 agatggatca tttctgttaa cgaagaggga agagcttgac cagaaagtaa agcagcttct
    53641 gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt cttcgatgag ctgcgaaata
    53701 tacaattgat tgatcacagc ttcagtttct ttgaatgcca aaacagggtc taaaatgatt
    53761 gggatatttt gttttgtttt tatgaagtct cgaacaacca tgattgcttc acttgaattc
    53821 aataacccaa ttttgatacc atctaatgaa gtcattttat caatcgaaac taactgtcgt
    53881 ttaagcaatt ccaaagatat agattccagc ataaatcgct cattaatatc tagtgcccct
    53941 atagaagtca aggcgcttag tccgaataga ccaaaattct caaaagtttt taaatctgtt
    54001 tggattcctc caccagcaaa tggatcagaa ccgccaattg tcaagattct tttcataaca
    54061 ttttcctcca tgaataagag tataacgaaa ttttcaaaaa agaaaaacag ccaattggca
    54121 tatttttatc tcatccaatt taaaaaaaca aaaagaaaag aaagaccaaa atggatcttt
    54181 ctttccttaa atagttagga cttttgtgat tttaattctt tctcgatttg aagccgttgc
    54241 actttcattg tagctgttcg cgggattgca tcaaaatcac ggatgatagg gacgttcaag
    54301 cgaggaagtt catggacttg ctcccaccat cgattccaat ccatttcttt atcaggagct
    54361 aaagcaataa tcggttgtgg agaatgattt ttatccctta caatcactac ttctgccaaa
    54421 aaatctaacg aatctaataa aaagtcttca atagctaatg tactattgat cgtttcgatc
    54481 aaatccactt gccgatcttt caaaaatagg tggccatgct tatccataaa accgtaatct
    54541 ccgctatccc accaatcgcc ataaacattt tcctggaacc gtgcatcttc cttgtaataa
    54601 gtcaaagccc gtcctttaga cagtaattgt atatgaccat ctgtgttttc aggtaggaca
    54661 ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt ccagtccaac gcccatatct
    54721 cttgcatcac ttgttttcaa actctctagt gtatgagcct taagaatcat tggtccgcat
    54781 tcactttggc catatacttg aagaaatacc gcatcctgtg ccatcgacgt tcgcaaaaat
    54841 gtcttcattg tctggttgtt tatagcatca aaagtggagt gatagtatcg gatacccgaa
    54901 aaagcttctg gatgctcctt agcagtaaaa cgccactgaa caaagttatt cggatgggtc
    54961 tctagtgcaa ttggccggtg agcttccaac atatgaacaa cttttgaact ttgtgcgttt
    55021 gctaaaggca tcataggaaa tcccatggac atcaaagaag agataccaat attgaatctg
    55081 gaatgcactg gagagatgtg aaaagcgatt aatttctttt ccgaaatttt tgtgaaaact
    55141 gttttttgcc atttcgtccg ccatcccata gaatgagcag aatgacaaat aagtttaggg
    55201 ataccagttg taccagaagt atgggtcata taagaaattt catttttgtc taaaaatgat
    55261 tgagaaacag acaaagcagg ttgttccatt aaataaagaa tagg
//
LOCUS       DAJSVF010000010        55218 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSVF010000010 DAJSVF010000000
VERSION     DAJSVF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.010"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..644)
                     /locus_tag="ON550_000568"
     CDS             complement(<1..644)
                     /locus_tag="ON550_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3953678.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIR"
     gene            864..1952
                     /locus_tag="ON550_000569"
     CDS             864..1952
                     /locus_tag="ON550_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR3953679.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            2006..3532
                     /locus_tag="ON550_000570"
     CDS             2006..3532
                     /locus_tag="ON550_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3953680.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            3534..4739
                     /locus_tag="ON550_000571"
     CDS             3534..4739
                     /locus_tag="ON550_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3953681.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            4996..5973
                     /gene="guaC"
                     /locus_tag="ON550_000572"
     CDS             4996..5973
                     /gene="guaC"
                     /locus_tag="ON550_000572"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR3953682.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            6237..7661
                     /locus_tag="ON550_000573"
     CDS             6237..7661
                     /locus_tag="ON550_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3953683.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            8122..9231
                     /gene="pdhA"
                     /locus_tag="ON550_000574"
     CDS             8122..9231
                     /gene="pdhA"
                     /locus_tag="ON550_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR3953684.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            9234..10211
                     /locus_tag="ON550_000575"
     CDS             9234..10211
                     /locus_tag="ON550_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR3953685.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            10236..11879
                     /locus_tag="ON550_000576"
     CDS             10236..11879
                     /locus_tag="ON550_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR3953686.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            11887..13293
                     /gene="lpdA"
                     /locus_tag="ON550_000577"
     CDS             11887..13293
                     /gene="lpdA"
                     /locus_tag="ON550_000577"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR3953687.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            13455..13730
                     /locus_tag="ON550_000578"
     CDS             13455..13730
                     /locus_tag="ON550_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR3953688.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            13736..14503
                     /locus_tag="ON550_000579"
     CDS             13736..14503
                     /locus_tag="ON550_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR3953689.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            14671..16500
                     /gene="typA"
                     /locus_tag="ON550_000580"
     CDS             14671..16500
                     /gene="typA"
                     /locus_tag="ON550_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR3953690.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            16761..18533
                     /gene="recQ"
                     /locus_tag="ON550_000581"
     CDS             16761..18533
                     /gene="recQ"
                     /locus_tag="ON550_000581"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR3953691.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            18647..18949
                     /locus_tag="ON550_000582"
     CDS             18647..18949
                     /locus_tag="ON550_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR3953692.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            18952..20115
                     /locus_tag="ON550_000583"
     CDS             18952..20115
                     /locus_tag="ON550_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3953693.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            20141..23569
                     /locus_tag="ON550_000584"
     CDS             20141..23569
                     /locus_tag="ON550_000584"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR3953694.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            23590..24714
                     /locus_tag="ON550_000585"
     CDS             23590..24714
                     /locus_tag="ON550_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953695.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            24704..25141
                     /locus_tag="ON550_000586"
     CDS             24704..25141
                     /locus_tag="ON550_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3953696.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            25125..25463
                     /locus_tag="ON550_000587"
     CDS             25125..25463
                     /locus_tag="ON550_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR3953697.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            25582..26142
                     /gene="rsmD"
                     /locus_tag="ON550_000588"
     CDS             25582..26142
                     /gene="rsmD"
                     /locus_tag="ON550_000588"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR3953698.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            26139..26630
                     /gene="coaD"
                     /locus_tag="ON550_000589"
     CDS             26139..26630
                     /gene="coaD"
                     /locus_tag="ON550_000589"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR3953699.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            26620..27663
                     /locus_tag="ON550_000590"
     CDS             26620..27663
                     /locus_tag="ON550_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR3953700.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            27822..28550
                     /locus_tag="ON550_000591"
     CDS             27822..28550
                     /locus_tag="ON550_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR3953701.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            28666..28911
                     /locus_tag="ON550_000592"
     CDS             28666..28911
                     /locus_tag="ON550_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3953702.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRDRFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            28908..29126
                     /locus_tag="ON550_000593"
     CDS             28908..29126
                     /locus_tag="ON550_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3953703.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            29262..29801
                     /locus_tag="ON550_000594"
     CDS             29262..29801
                     /locus_tag="ON550_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR3953704.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            29794..32067
                     /locus_tag="ON550_000595"
     CDS             29794..32067
                     /locus_tag="ON550_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR3953705.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            32105..33151
                     /gene="holA"
                     /locus_tag="ON550_000596"
     CDS             32105..33151
                     /gene="holA"
                     /locus_tag="ON550_000596"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR3953706.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(33240..33494)
                     /locus_tag="ON550_000597"
     CDS             complement(33240..33494)
                     /locus_tag="ON550_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR3953707.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(33894..34163)
                     /locus_tag="ON550_000598"
     CDS             complement(33894..34163)
                     /locus_tag="ON550_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953708.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(34633..35088)
                     /locus_tag="ON550_000599"
     CDS             complement(34633..35088)
                     /locus_tag="ON550_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR3953709.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(35226..37154)
                     /locus_tag="ON550_000600"
     CDS             complement(35226..37154)
                     /locus_tag="ON550_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3953710.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(37351..38184)
                     /locus_tag="ON550_000601"
     CDS             complement(37351..38184)
                     /locus_tag="ON550_000601"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3953711.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(38376..39080)
                     /locus_tag="ON550_000602"
     CDS             complement(38376..39080)
                     /locus_tag="ON550_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3953712.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(39103..39729)
                     /locus_tag="ON550_000603"
     CDS             complement(39103..39729)
                     /locus_tag="ON550_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3953713.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            40055..42430
                     /locus_tag="ON550_000604"
     CDS             40055..42430
                     /locus_tag="ON550_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR3953714.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(42454..42945)
                     /locus_tag="ON550_000605"
     CDS             complement(42454..42945)
                     /locus_tag="ON550_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR3953715.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            43132..44052
                     /locus_tag="ON550_000606"
     CDS             43132..44052
                     /locus_tag="ON550_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3953716.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            44206..44550
                     /locus_tag="ON550_000607"
     CDS             44206..44550
                     /locus_tag="ON550_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR3953717.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(44740..45240)
                     /locus_tag="ON550_000608"
     CDS             complement(44740..45240)
                     /locus_tag="ON550_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953718.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            45768..45851
                     /locus_tag="ON550_000609"
     tRNA            45768..45851
                     /locus_tag="ON550_000609"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45802..45804,aa:Leu,seq:cag)
     gene            complement(46175..46813)
                     /locus_tag="ON550_000610"
     CDS             complement(46175..46813)
                     /locus_tag="ON550_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3953719.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            47148..47582
                     /locus_tag="ON550_000611"
     CDS             47148..47582
                     /locus_tag="ON550_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3953720.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            47605..47925
                     /locus_tag="ON550_000612"
     CDS             47605..47925
                     /locus_tag="ON550_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953721.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            48066..48257
                     /locus_tag="ON550_000613"
     CDS             48066..48257
                     /locus_tag="ON550_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953722.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(48328..48585)
                     /locus_tag="ON550_000614"
     CDS             complement(48328..48585)
                     /locus_tag="ON550_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR3953723.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            48743..49006
                     /locus_tag="ON550_000615"
     CDS             48743..49006
                     /locus_tag="ON550_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953724.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            49030..49218
                     /locus_tag="ON550_000616"
     CDS             49030..49218
                     /locus_tag="ON550_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953725.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(49301..49486)
                     /locus_tag="ON550_000617"
     CDS             complement(49301..49486)
                     /locus_tag="ON550_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953726.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            49701..50072
                     /locus_tag="ON550_000618"
     CDS             49701..50072
                     /locus_tag="ON550_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3953727.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(50222..50294)
                     /locus_tag="ON550_000619"
     tRNA            complement(50222..50294)
                     /locus_tag="ON550_000619"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50259..50261),aa:Gln,seq:ctg)
     gene            complement(50340..50768)
                     /locus_tag="ON550_000620"
     CDS             complement(50340..50768)
                     /locus_tag="ON550_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953728.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(50828..51586)
                     /locus_tag="ON550_000621"
     CDS             complement(50828..51586)
                     /locus_tag="ON550_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3953729.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            51881..52078
                     /locus_tag="ON550_000622"
     CDS             51881..52078
                     /locus_tag="ON550_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953730.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            52184..52666
                     /locus_tag="ON550_000623"
     CDS             52184..52666
                     /locus_tag="ON550_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953731.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(52724..53320)
                     /locus_tag="ON550_000624"
     CDS             complement(52724..53320)
                     /locus_tag="ON550_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3953732.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            53707..55173
                     /locus_tag="ON550_000625"
     CDS             53707..55173
                     /locus_tag="ON550_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR3953733.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        18229 a         9447 c        11516 g        16026 t
ORIGIN      
        1 cgaataaaat caccgacaat tcggtgaaca atagtcatcg gaggtttatg gtcgatggtt
       61 ccatcttttt ttagggttgg tttacgattg attgattcca aaaatccttc ggagcgtagc
      121 ttggtcggtg tggagttctc tgaaccagca agttctagga ttctgatttc ctgtttcttt
      181 gaatttgtga agtgattgct gacaaataaa cccgcccgat ttccttctgc tttgaaactg
      241 gggccaatgt gggtgacgaa aagctcggga tcattcgttt gaatatgtct gtcgacaagt
      301 atgacaggga tccctgctcg ctttgcttct tttaatacag agtcccaccc ggattcttgc
      361 aaaggagtaa aaatgatcat atccacttta taggcaatga acgtccgtat atcttggatt
      421 tgccgttcct gattcatgta gccatttcgg tagagaactt ggtaatcttc tttttcaagc
      481 gcagtgataa ttgattcagt atgctttttc cgccagctac tctccgttcc tgattgcgaa
      541 aaaccaacga ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc catcatttgg
      601 taagcgatta caacaagggc taaaaaaaat aaaacacgtt tcactttaaa tcactccatc
      661 tggatttgtt agtactatct tatcagataa aagggaaaat caaagtcaaa aggaatcaat
      721 cgaaaaaaat ccaagaaact taaaaaaatc caagacaaaa aaagtacacc taaaaaattt
      781 aaagtaaatc ccataaaaaa atcaatgtta acgctttctt tattagctat actcggtgta
      841 tcaattgaaa tgggggataa aaaatgaaaa acaaatttag aaagattgtt gggactatcg
      901 ctattttgac cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct gcagatgata
      961 gtacggggta tgtaggaatc gctatgccga ccaaatcagc agaaagatgg atcgctgatg
     1021 ggaacaacat ggtctcggaa ttagaaaaac tgggttataa aacagatctt caatatggag
     1081 aagataaagt agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa ggggtagata
     1141 cacttgtgat tgcttctatc gatggttcgg ctttaactga tgtgttagct aaagcaaaag
     1201 aagcagatat caaggtgatc gcctatgatc gtttactgat gaattcagaa aatgtcgatt
     1261 attacgcgac ttttgataat tttggagtag gtgtatcgca agcagcttat attgaagagc
     1321 acttaggatt gaaagaagga aaaggaccat tcacgattga attatttggt ggctcgcctg
     1381 acgacaacaa cgcactaatc aattacaacg gagtgatgtc tgttttacaa ccatatatgg
     1441 acaacggtca gttggtcgtg ccatctggac agacgagttt cagtcaaatc gctacgcttc
     1501 gctgggatgg ttctacggcg caagcaagga tggataatct gttaagcgca aattatactg
     1561 accaaacatt ggatgctgtt ttgtcgccat atgatccaat aagtttagga atcatttcgt
     1621 cattaaaagg cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt actggacaag
     1681 atgcgacagt tgctggcgtt aaatcgatca ttgccgggga acaaacacaa acgatcttta
     1741 aagacacacg tatcttagca aaaaatacga ttgaaatgat caaagcaatc agtgatggag
     1801 aagaggttcc ggtcaatgat actgaaactt atgataatgg ggtgaaaact gtccctacgt
     1861 atttagcaaa tacagtatct gtcgataaag ataattatca agcagagctg atcgacaccg
     1921 attattataa agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa ctaaagagat
     1981 ctaagtcaga gagaggataa gaattatggc ggattgtatt ttaaaaatgg cagatatcac
     2041 caaaaaattt tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg aacgtgggga
     2101 aatccatgca ttgtgcggag aaaacggagc gggaaaatca acattgatga atgtcttgag
     2161 cggcttgtat ccatatggct cctatgacgg ggaaatcttt tatgatggaa agctgtgtca
     2221 atttaaagat ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc aagaattagc
     2281 actaagtcct tatctttcaa taaaagagaa tatttttctc ggaaatgaac aagcagaaag
     2341 aggcgtgatt gattgggaac tgacagaaaa gaaaacatat gaactgctga ataaagtcgg
     2401 tttgtctatc gacccgaatc ttctagtatc ccagattggc gtagggcaac agcaactagt
     2461 tgaaatagct aaagcattat caaaatccgt ccgtctgttg attttggatg agcctaccgc
     2521 tgcattgaat gaagaagaaa gcgcgaattt gctgagattg atccgagagt tgaaagagca
     2581 aggtgtaacc tccatcatta tttcacataa gctgaatgaa atcgtggata tcgcagatcg
     2641 aatcaccatt ttacgggatg gacagacgat tgagacacta gaaaatgaaa tgatcagcga
     2701 agagcatatt attcgcggaa tggtgggcag agatttagtt gatcgttatc ctgaaagaaa
     2761 accggatatt ggagaagttt attttaaagt agaagactgg acggtctatc accctcttga
     2821 tcacgagcga atcataaacg accacatcaa cttgcatttg aaaaaaggag agattgtagg
     2881 aatagctgga ctgatgggag cgggcagaac tgaattcgct atgagtctat ttggacattc
     2941 ttatggaagc aagatatccg gacgtatatt aaaggaaggg gcagagatat cagttaagac
     3001 cgttccttca gccattgcaa acggactcgc ttatgtctcg gaggaccgaa aatctttagg
     3061 actgaatctg ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg ataaaataag
     3121 tcaaaaagga gtattagata aagaaaaaga agtaactgag gcagaaaatt tccggaaaaa
     3181 gatgcgtata aaaacaaact ccatccatca aattgtcagt agtcttagtg gtgggaatca
     3241 acaaaaagtc gttttatcca agtggctgat gacacaaccc gatattcttt ttttggatga
     3301 gccaacccgt gggatagatg ttggggcaaa atatgagatc tacacgatta tcgaagaaat
     3361 ggcagcaaat ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa tattagggat
     3421 gtgtgatcga atctatacga tgaacgaagg acgtattaca ggagaagtct cgcgagaaga
     3481 tgcagatcag gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt aagttggaac
     3541 aggcaaaaaa tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt agagcagctg
     3601 atctttttgg tcgctacagc atggtgatca ttttagcggg actacttatt gcgtttcaat
     3661 tgatgacgga tggtattttc tggcgtccat tgaatataac taaccttgtt ctgcaaaata
     3721 gccatatatt agtactagct gcagggatgt tattagttgt gctcttaggt catgtggatt
     3781 tgtcggtagg gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg atggtcaatc
     3841 gccaaatgaa cccgtggatg acagttatcg catgtcttgc agtaggcggg ctgatcggcg
     3901 catggcaagg tttttgggta gcttatgcag ggataccggc gtttatcgtg actttagcag
     3961 gattgctgat gtttcgtgga ctgacacaag ttgttctagg aggccaatct ctagcacctt
     4021 ttccagctgt tttccaaaaa atgtctacgg gttatttacc agacttaagc gaaggaagca
     4081 gccttcactt cttgacattg attttaggaa tgatcatcac gatcgtgtta gttgttggtc
     4141 aatggcgaac aagaaatcgt cggaaaaatc atttatttga agtggaatct ccaacagctt
     4201 tcttggtaaa ggcggtattt acagcagtcg ttgtactagg aataacttat atctttgcct
     4261 cttatcaagg gtatccgatt attttgatca ttcttggttt gatcgttgca gcatatgggt
     4321 ttttgacaaa taaaacagta gcaggtcgac agatttatgc gacgggcgga aatcgaaaag
     4381 cggctgaact atcagggatc aaaacaaagc gaatcacgtt ctgggtattc gtcaacatgg
     4441 gagtcatggc agctttagct ggattagttt tagctgctcg attgaatgcg gcaacacctc
     4501 aagcaggtac atcgatggaa ctggatgcaa tggcggcagt atattttggt ggtgcttcga
     4561 cttccggcgg cattggaaca atcatgggtg cgattgtcgg tggcttggtc atgggtgtat
     4621 tgaacaatgg catgtcgatt ttaggcgtcg gggtagactg gcagcaggca atcaaaggat
     4681 tgatcttgct tttagccgtt gtgctagact tgtataataa gaaaaaacga acaacgtagg
     4741 agaacgccgg ttattcagaa aaaagactgt gacaagacat ttgtcacagt ctttttttcg
     4801 ctaaaagcga acattcattg tttcgtttgt atagactaaa aataatatat gtttttttac
     4861 cattcgtata aattctcttt ttgctcttga tttttttgct taattgagtt aaattacata
     4921 gggcgctaaa aaaagccttg attaaattaa taatgttcgt gttttgctgg atgtgaacat
     4981 caggaggata atgtaatgaa agtatttgat tacgaagata ttcaattaat tcctaataaa
     5041 tgtatcgtaa atagccgttc tgaatgtgat acaacggtca cattaggtaa acatacattc
     5101 aaaatgccag tagtgcctgc caacatgcag acgattattg atgaaacaat cgcagaattc
     5161 ttagcggaaa atggttattt ctatatcatg catcggtttg atgaagcagc tcgaattccg
     5221 tttatcaaaa aaatgaaaaa gcgtggattg attacctcga tcagtgttgg cgtcaagaaa
     5281 gaggaatatt cctttataga gaagttggca gaagaatcgc taaatcctga ttatatcact
     5341 atcgacatcg cgcatggaca tgctaactca gttattgata tgattcagca tattaaaaaa
     5401 tatttgcctg aaacttttgt gattgccggt aatgttggta caccggaagc cgtccgtgaa
     5461 ttggaaaatg cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa agtatgtatt
     5521 acaaaaatca agacaggctt tgggactgga ggctggcaac tggcagctct tagatggtgt
     5581 gcgaaagcag ctcgcaagcc gatcatcgct gacggtggga tcagaacaca tggcgatatt
     5641 gcaaaatctg tccgttttgg tgcgacaatg gtcatgattg gttcattgtt tgccggtcac
     5701 gaggaatctc ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga atactttggt
     5761 tctgcttcag aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa aatctggatc
     5821 caacacaaag gatctttgaa agatacacta gtggaaatgc aacaagacct tcaatcatct
     5881 atttcttatg ctggaggaag agatctggaa gccattcgaa aagttgacta tgtcatcgtg
     5941 aaaaattcga tttttaatgg agacacaatc taaaaaataa ataaaatatt aggatgttgt
     6001 gaaaaagttg tcctgcatta agtaataaga acagccaaac aaaaatagtt ctttatatgt
     6061 tttgtttggc tggaattatt atcgcagata caagcttttg aacatcatta ttcagaataa
     6121 gggactgtga taagattttt gtcacagtcc cttatgtttt gcaaaaaaag tttttctttt
     6181 ttctcgtaaa ttcggtaaaa tcaaaagaaa aaatagacaa tagaaaagag aagcttatgt
     6241 gggaaaaagt agagaaaaaa caaggaccga tttatcgtca aattatggaa cagattatga
     6301 gagaaatcga aaacggaagg atcaatcctg gaagtcaact tgaatcagaa cgaaaactag
     6361 ggcaactttt tggtgtgaat cgttccaccg ttgtccatgc attggaagag cttagggagc
     6421 ttggggtact aacgagcaaa cgtggcagcg gatggtttgt taatcagact gaatggggga
     6481 aattcgctgt tccaagaatc gattggcggc aaatgatctc tcctcgttat gagcagacgg
     6541 actggtatga gcaaaaaata agagaagcaa agcagatcca aaaaacatcg tttcttgatt
     6601 tatacgctag cgagatgcca gatgagcttt tgccgaattt tgagtttcct tcttattctc
     6661 ttgaagaaat cttagcggaa gaaaaagaaa tggatctttt aggctatact ccgttacgta
     6721 acaaaatcaa acggtatttg gaaagtaaat ttgaattggc attttccccc aaccagcttc
     6781 ttgtgactgg tggcggtcag caagcaatct ttttgatttt acaaacgatt ctttcagctg
     6841 gagaggggat agctgtagag tcaccttctt ttttttaccg attacctctt ttcaaagctt
     6901 caggaacgcg tttatttggg ataccgatgg atgaagaagg gatcgactta gaaaaactga
     6961 aagaatcgat ccataaaaat aaattgaaag cagtgcttat caacccgaat tttcaaaacc
     7021 ctacaggaaa agtgatgagt caaaaacgac gagaaaagct agtagaattg tgtcgacagt
     7081 atcggcttcc aattattgaa gacgatgtat ttacggatct ttcatttcta aaccaagaag
     7141 aagtccttcc tatacgaacg attgatccgg ataatgtgtt atacgtcggt tcgttatcta
     7201 gggttttagg aaaaacaacg aaaatcggtt gggtgatagg acctgagact tttatctcgc
     7261 aactagctca agcgcaagaa atgatggagt tttcgatgaa tatccttaca caaatcacag
     7321 tggctaatgt atttaatgaa gcaattgatt tgaaaatgca agatgtaaga aaaaagttga
     7381 aagaaaaaag ccgactgata agcgcttggg gagattcaca atcgtttttc aagcttcatt
     7441 caattttagg tggttactat gcctggatta cttgggatgg gaaaaaacta gaccccgaac
     7501 tagcagaaaa agtagtagat atgggtcttg gaattgcacc aagtctactt tttgggcaag
     7561 atataaatgg aatcagaatc aattacagta ggctggatga aaaccaaata ccgttgtttt
     7621 attcaaagat gaaaattcta gaaaagtggt taacagaata atttcacaaa gtaaatagat
     7681 taaagaatat aacagcttgg atttaaattt caagctgttt cttttttttc ggaaaaagaa
     7741 tacgatataa agaaactaag aaaaaaccat tatttgtacg tccgttcacg atgtgtaaat
     7801 aaaataaatg tttgcaacaa aactgtataa caccaacgtt tttaaataga aacagatggt
     7861 tgtaagtttg attagaaaat agaaaaaatg cagatgaaat ataaaacgct ttccttattg
     7921 tgattttatt cactaatgct gttaatatgc ggattggcat aatttccttt catcgtgaaa
     7981 atttgtgctt ttagcttgta tttctcataa atccatgata atttattaat gaagacggtt
     8041 aaatagtgat acagttttat tcactgtttc aatcgcagaa tgaagagatt ttcttataga
     8101 tgagagagga atgtgtgaca aatggcaaac aaaaaagcac cgatcgactt ccaagcactg
     8161 ctagatgaag tcaatgcaga tttcccagta tatcaagcac ttgatcaaga tggaaacgta
     8221 gttaacaaag atttagtacc tgatttatcc gatgatgaac tcgttgaatt aatgactcat
     8281 atggtatggt ctcgtgtatt agaccaacgt tcaacagcgc tgaaccgcca aggacgccta
     8341 ggcttttttg caccaactgc tggacaagag gcaagtcagc tggctagtgc ttacgcattt
     8401 gataaagaag atgttcttct tccaggttat cgggatgtac ctcaactagt aaaacatggt
     8461 ttacctttat ctcaagcttt tttatggtct cgaggacatg ctgcaggtaa ttcttatccg
     8521 gaagaattga aagctctacc accacaaatt atcatcggtg cacaatatgt acaagcagcc
     8581 ggtgttgcat taggtttgaa gaaacgaaac aagaaaaatg tcgtcttcac ttatactggt
     8641 gatggcggtt catcacaagg tgatttttac gaagcaatca atttcgctgg cgcttaccat
     8701 gcaaatgccg tattttatat ccaaaacaac gggtatgcga tctctacacc tcgtgaaaaa
     8761 caaactgcag caaaaacact tgcacaaaaa gctgtcgcag ctgggattcc tggtattcaa
     8821 gtcgatggaa tggatccgct tgcagtatat actgtatcta aaatggcacg ggaatgggcg
     8881 atcagtggaa atggaccagt cctaatcgaa acactgactt atcgttatgg accacataca
     8941 ctatctggtg acgatccaac ccgctaccgt acaaaagaca cagatgacga ttggcagaaa
     9001 aaagatccat tgatccgctt ccgtaaatat ttgacggaaa aaggactttg gtctgaagaa
     9061 aaagaagaac aagtcatcga agcaactaaa gaagaaatca aagcagctat tgctgaagcc
     9121 gacaaagtac caaaacaaaa ggtttcagat ttcttgaaaa acatgttcga agtatctcca
     9181 caatcaatca aagaacaaat tgcaatttat gaagcgaagg agtcgaaata atcatggcac
     9241 aaaaaacaat gatccaagca atcacagatg ccttagcact tgaactagaa aatgacgaga
     9301 atgtcgttgt ttttggtgaa gatgttggga aaaacggagg agttttccgg gcaactgaag
     9361 gattgcagga aaagttcgga gaagaccgag tattcgatac tcctttagct gaatctggaa
     9421 tcgcaggatt gagtttcggt cttgcgttag aaggatttcg tccagttcct gaaatccaat
     9481 tctttggttt tattttcgaa gcaatggacg aagtagtagc tcaaatggct cgtacaagat
     9541 atcgaatgag cggcactaga aatctaccca tcacaattcg ttcgccattt ggtggcggtg
     9601 ttcatacacc agaattgcat tcggataatt tagaaggttt gatcgctcag tctcctggta
     9661 ttcgagtagt gattccttcc aacccttatg atgcaaaagg actattgatt tcttctatta
     9721 gaagtaacga tccggttgtt ttcctagaac acatgaaact atatcgctca ttccgagaag
     9781 aagtgccgga tgaagcttac gaagttcctt tggataaagc agcagttact cgtgagggaa
     9841 cagatgtatc tattatcaca tacggagcaa tggtgcgtga agcaatcaaa gcagcagaca
     9901 atcttgcaaa agaaaatata tctgttgaaa tcattgatct tcgtacagtt gcgccattag
     9961 atgtagaaac gatcattcaa tccgtggaaa aaactggtcg cgtagttgtt gtccaagaag
    10021 cacaacgaca agcgggcgtt gccgcgcaag tagtttctga gatctcagaa cgcgctatcc
    10081 tttcattaga agccccaatc ggacgagttt ctgcacctga tacagtcttc ccatttggac
    10141 aagcagaaaa tgtctggttg ccaaatgcaa aagatatcga agacaaagta aaagaaatcg
    10201 cggaatttta agaaaagaag ggacgaaaca aagtcatggc ttatcaattt aaattgccgg
    10261 atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa ccaggagata
    10321 cgatcaatga agatgataca ttactagaag ttcaaaatga caaatcagta gaagaaatcc
    10381 cgtcacctgt tacaggaacg gttaaaaatg tgattgttcc agaaggtacc gtagcaaatg
    10441 ttggggatgt attagtagaa atcgatgcac caggacacga agacaatgaa ggggacagtg
    10501 gtgtagccgc agaatcccaa actccagcta aaccggctgc tgaacccact gttgatacag
    10561 aatcagctgg atcgtcaagc gaaggagtct tccaatttaa gttaccggat atcggtgaag
    10621 gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag
    10681 atgatacatt actagaagtt caaaatgaca aatcagtaga agaaattccg tcacctgtca
    10741 cagggacagt taaaaacgtg attgttccag aaggcactgt agcaaatgtt ggggatgtat
    10801 tagtagaaat cgatgctcct ggacacaata gtgcaccgtc aactagtgcg ccatcagctg
    10861 aagcaccaaa agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa gctgccgatc
    10921 caaataaacg tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa aaagatgtag
    10981 atatctcaca agtaactgca actggaaaag gtggcagagt aacaaaagag gacatcgaaa
    11041 acttcttggc aggtggtcca agcagtgcac cagcgaaatc agaagcgcca gaagcggcag
    11101 caccgaaaga agcagctcct gcagcagaaa gcaaaccagc tgcaccagca aaaccattca
    11161 agtccaactt aggagacttg gaagaacgtg tagcaatgac gccaacacgt aaagctatcg
    11221 ctaaagcaat ggtcaacagt aagcatacag ctccacacgt gacattgcat gatgaagtag
    11281 aagtgtctaa actatgggat aatcggaaac gatttaaaga agttgctgct gcaaatggta
    11341 cgaaacttac tttcttgcca tatgtcgtga aagctttaac agcgactgtt aagaaatacc
    11401 cagtattgaa tgcatctatc gacgatgcaa accaagaaat cgtttataaa cattactaca
    11461 atatcggtat tgcaactgat acggatcacg gactatatgt accaaatgtg aaagatgcag
    11521 accgcaaagg aatgtttgct attgcagacg aaatcaatga gaaagcaaaa ttagctcatg
    11581 acggcaaact atctgcagaa gatatgcgca atggaacaat tacgatcagc aatatcggat
    11641 ctgtaggtgg cggttggttt acaccagtca tcaactatcc agaagtagct attttaggtg
    11701 taggaacgat tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt gtcggtcgtg
    11761 tgatgaaact gtcattaagt ttcgatcacc gtatcgtaga tggagcaact gcgcaacaag
    11821 caatgaataa tatcaaacgt cttttagctg atccagagct attaatgatg gaaggatgat
    11881 aaacaaatgg tagttggaga ttttgctgta gaattagata cagttgtaat tggtgcggga
    11941 cctggtggct atgtagctgc catccgcgct gctgaaatgg gacaaaaagt agcaatcatt
    12001 gaaagagagt atattggcgg tgtttgtttg aatgtcggct gtattccttc aaaagcactg
    12061 atagctgcag gacaccatta tcaagagtca ctagattctg aaatgtttgg agtaactagt
    12121 gaaaacgtca aactagattt tgcaaaaaca caagaatgga aagaaaacaa agttgtccat
    12181 acgttgactt ctggtgttgg tttcctatta aaaaaacata aggttgaaac aatcgaagga
    12241 gaagcgttct tcgtagatga tcacacacta cgcgtcattc atccagattc agctcaaact
    12301 tattcgttca ataacgcaat catcgcaaca ggttcccgtc caatcgagat tccaggattt
    12361 aaattcggag gacgtgtgtt agattctact ggcggattag cattgaaaga agtacctaag
    12421 aaatttgtga tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc gtacgcaaac
    12481 ttaggagcag aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac ttacgaaaaa
    12541 gatctagtaa aattagtaga agatgatttc aagaaaaaag gcgtaacagt cgtaacgaat
    12601 gcaatggcga aagaagcagt cgataacggt gacagcgtga cagtgaaata tgcagtggac
    12661 gggaaagaag aatccgttac tgctgattat gtgatggtca ctgtaggtcg tcgtccgaat
    12721 acagatgata tgggcttaga acaagcgggt gtcgaagtag gagaacgagg attgatcacg
    12781 gtggataaac aaggacgtac gaacgtacct aacatctatg ccatcggtga tatcgttcca
    12841 ggggcggcat tagcacacaa agccagttat gaagcaaaaa tcgctgctga agcaatctct
    12901 ggcaaaaaag tagctgtaga ttataaagca atgccggcag tagccttcac tgatcctgaa
    12961 ttggcttctg taggtatgac gatcaaagaa gcaaaagatg ctggtatcga agcaaaagca
    13021 tataaattcc cattttcagg gaacggtcgg gcattatcgc ttggtaaaac agaaggattc
    13081 atccgtctag ttacgacgat cgaagataac gtgcttattg gcgcgcaaat cggtggagtc
    13141 ggcgcaagtg atatggtctc tgaattagct ttagcaatcg aatcaggaat gaatgctgaa
    13201 gatatcgcct tgacgattca tcctcatcca tctcttggcg aaatcgtcat ggatgcttcg
    13261 gaattagcat taggtttacc aatccatatt tgatttgaat aatacaggcg aaacgcaact
    13321 gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa
    13381 aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa
    13441 taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg
    13501 aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa
    13561 acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg
    13621 aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag
    13681 tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga
    13741 acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc
    13801 tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga
    13861 tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat
    13921 attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat
    13981 cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct
    14041 cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga
    14101 acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc
    14161 agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa
    14221 taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc
    14281 tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag
    14341 cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg
    14401 tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg
    14461 tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca
    14521 ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt
    14581 actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat
    14641 aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc
    14701 gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa
    14761 tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta
    14821 gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt
    14881 cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc
    14941 atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa
    15001 acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac
    15061 aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc
    15121 atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta
    15181 aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt
    15241 tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa
    15301 ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta
    15361 tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt
    15421 aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa
    15481 gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa
    15541 acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca
    15601 ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta
    15661 accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga
    15721 gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg
    15781 tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa
    15841 gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat
    15901 acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg
    15961 caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc
    16021 ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac
    16081 catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg
    16141 gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa
    16201 cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa
    16261 aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta
    16321 cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa
    16381 gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc
    16441 ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa
    16501 tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt
    16561 gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg
    16621 ccgaaagacg tttaaaagac ttttaaaaaa cttatttcta gagagttttt gtttcataat
    16681 aagataatgt ttcacgaaag gagatcaact tgtccttcga agaaaatcga agtaaaatgg
    16741 caagagaaag gagcgggaag atgaacgact tattaaaaca atactttggc tatgatgagt
    16801 ttagacctgg tcaaaaagaa attatccaga aagtcattga tcaggaaaat gtattaggaa
    16861 tcatgcctac cggaagtggg aaatcaatct gttaccagct tccagcttta ctattagatg
    16921 ggcttactgt ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg gacgcggcga
    16981 atcaattagg tatacccgcc acttttatca atagctcgtt agatggttac gaaacagcta
    17041 gacgcttcca agaaatcgat agacagcagt accgtttact ttatatcgct cctgaacgtt
    17101 ttatcatgcc ggattttatc caagcaatga agcgatggaa cgtccgtatg atcgctattg
    17161 acgaggcaca ttgtatctct cagtgggggc atgatttccg cccaagttat ttacagatgg
    17221 cgaaccagct agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca gctacagcga
    17281 ctgtgcaagt agctgctgac ataaaaagac ttttaaaaat tccagaaaac aaccatattc
    17341 agactggttt tgaacgagaa aatctacgat ttcaagtaat taaagatcaa aaaaaagaac
    17401 aatatctaat cgaatattta aagatcaaca aaaatcaatc tggtatcatt tatgcagcaa
    17461 cgagaaaaga agtagaccgt ctctatcatt tgcttaaaaa attcgatttt tccgtgggta
    17521 gatatcatgg tggtctaaat gagaatgagc gaacagaaat gcaagaagcc tttttatacg
    17581 atcgcttgca gctgattgta gcgacaaatg catttggtat gggtataaat aaaagcaatg
    17641 tccgttttgt tatccattat caaattcctg gctctctaga agcatactat caggaagctg
    17701 gtcgggcggg aagagatggt ctttccagtg aagccatcct gcttttctct cctcaagata
    17761 tccaagtcca aaagtttttt atccagcagt cccaaagaga agaagggcaa aagcaaaaag
    17821 aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat
    17881 acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag
    17941 atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac
    18001 gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac
    18061 aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat
    18121 cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca
    18181 tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac
    18241 aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg
    18301 atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc
    18361 ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa
    18421 gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg
    18481 aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac
    18541 gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa
    18601 atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc
    18661 gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg
    18721 aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac
    18781 acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa
    18841 gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga
    18901 ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag
    18961 aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt aattttatgt
    19021 gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa
    19081 aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc
    19141 gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc
    19201 atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg
    19261 gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc
    19321 atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat
    19381 tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct
    19441 attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta
    19501 gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt
    19561 acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag
    19621 tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg
    19681 catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta
    19741 ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca
    19801 atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt
    19861 atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg
    19921 tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact
    19981 ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg
    20041 ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag
    20101 agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc
    20161 taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac
    20221 tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc
    20281 atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat
    20341 ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga
    20401 aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact
    20461 gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg
    20521 gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt
    20581 tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg
    20641 catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga
    20701 agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa
    20761 gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag
    20821 agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat
    20881 gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg
    20941 gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga
    21001 agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat
    21061 cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc
    21121 tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga
    21181 cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg
    21241 ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt
    21301 tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa
    21361 aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat
    21421 gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga
    21481 tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa
    21541 atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt
    21601 ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa
    21661 gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa
    21721 tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt
    21781 tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt
    21841 ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga
    21901 tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct
    21961 tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt
    22021 tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg
    22081 gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc
    22141 agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta
    22201 ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga
    22261 tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac
    22321 gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta
    22381 ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg
    22441 gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct
    22501 gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat
    22561 gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga
    22621 aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca
    22681 tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt
    22741 taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga
    22801 tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt
    22861 gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa
    22921 gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt
    22981 cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga
    23041 cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa
    23101 tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg
    23161 tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat
    23221 cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg
    23281 tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga
    23341 accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt
    23401 gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat
    23461 ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg
    23521 agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg
    23581 tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca
    23641 ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa
    23701 caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct
    23761 acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga
    23821 ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca
    23881 tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct
    23941 gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact
    24001 atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa
    24061 gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt
    24121 tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg
    24181 aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg
    24241 gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg
    24301 cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt
    24361 cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg
    24421 gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat
    24481 gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt
    24541 catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat
    24601 gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg
    24661 ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga
    24721 gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac
    24781 gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt
    24841 gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta
    24901 tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat
    24961 ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca
    25021 aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc
    25081 gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata
    25141 gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat
    25201 taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg
    25261 tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt
    25321 ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa
    25381 taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa
    25441 tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact
    25501 tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta
    25561 ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta
    25621 aaaagtttag caggggcgaa caccagacca actacagata aagtaaaaga atccatattc
    25681 aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt
    25741 ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat
    25801 taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt
    25861 accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa
    25921 ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat
    25981 aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa
    26041 acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc
    26101 ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag
    26161 gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat
    26221 tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag
    26281 aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc
    26341 atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca
    26401 gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc
    26461 atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga
    26521 gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc
    26581 cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg
    26641 aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg
    26701 ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat
    26761 gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct
    26821 acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa
    26881 gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat
    26941 tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg
    27001 gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt
    27061 tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt
    27121 atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat
    27181 ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga
    27241 ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat
    27301 tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa
    27361 gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt
    27421 gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct
    27481 ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat
    27541 cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca
    27601 atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa
    27661 tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg
    27721 tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga
    27781 agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg
    27841 atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac
    27901 tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa
    27961 gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag
    28021 aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag
    28081 aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg
    28141 gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat
    28201 tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag
    28261 tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct
    28321 cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaatatc aatacagcca
    28381 ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc
    28441 gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta
    28501 tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa
    28561 aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa
    28621 gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa
    28681 gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat
    28741 attatgaata cggctgtcag cggcaataac agtcaagctc tacgagatcg ctttgccaaa
    28801 gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca
    28861 gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc
    28921 tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga
    28981 caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag
    29041 tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga
    29101 ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta
    29161 ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga
    29221 gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa
    29281 aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt
    29341 gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt
    29401 tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag
    29461 cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct
    29521 tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga
    29581 cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt
    29641 tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc
    29701 aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt
    29761 tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt
    29821 cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact
    29881 gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa
    29941 ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg
    30001 ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca
    30061 gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa
    30121 caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac
    30181 ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag
    30241 cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg
    30301 ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag
    30361 agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta
    30421 tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt
    30481 tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat
    30541 ttagggtggc tgtattattt atttcgacgt atcggatggc cactacacgt cagtattatt
    30601 ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg
    30661 cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc
    30721 accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt
    30781 tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt
    30841 gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg
    30901 ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac attactgatt
    30961 gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc
    31021 tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata
    31081 atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg
    31141 atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac
    31201 ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct
    31261 tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt
    31321 cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg
    31381 gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag
    31441 gggataagtg taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa
    31501 ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga
    31561 cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt
    31621 gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt
    31681 gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg
    31741 cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat
    31801 cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac
    31861 acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat
    31921 cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca
    31981 tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat
    32041 gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt
    32101 gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact
    32161 tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga
    32221 aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat
    32281 ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga
    32341 tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg
    32401 aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt
    32461 attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt
    32521 gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata
    32581 tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt
    32641 cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct
    32701 ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac
    32761 gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc
    32821 tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat
    32881 tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca
    32941 gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca
    33001 ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat
    33061 ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt
    33121 gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga
    33181 aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt
    33241 tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt
    33301 agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg
    33361 ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt
    33421 gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat
    33481 tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata
    33541 attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt
    33601 tttttcctta acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg
    33661 aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta
    33721 tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa
    33781 gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt
    33841 tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct
    33901 ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc
    33961 aactggttcc gacggttcca tttgccactt acttccagca catatttatt taggggcaaa
    34021 cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca
    34081 agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt
    34141 aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca
    34201 ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc
    34261 cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa
    34321 ttctatagga agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc
    34381 ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca
    34441 aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc
    34501 gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca
    34561 cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag
    34621 acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga
    34681 agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa
    34741 aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa
    34801 gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc
    34861 taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat
    34921 gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc
    34981 tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc
    35041 taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc
    35101 tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc
    35161 cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac
    35221 tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt
    35281 gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat
    35341 attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta
    35401 gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc
    35461 cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag
    35521 acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa
    35581 gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa
    35641 agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa
    35701 tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc
    35761 atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt
    35821 ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg
    35881 gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc
    35941 gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt
    36001 aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa
    36061 tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca
    36121 agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt
    36181 cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct
    36241 aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga
    36301 gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt
    36361 cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt
    36421 agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa
    36481 gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca
    36541 gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct
    36601 gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac
    36661 tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta
    36721 gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca
    36781 ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt
    36841 tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac
    36901 attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat
    36961 aaatccacat tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta
    37021 tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg
    37081 tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt
    37141 ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg
    37201 taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct
    37261 tgatgttgtt tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt
    37321 tctcaaataa gagaagtgtt ctggtttttt ctacattgca ataaaccaat aaccaatcaa
    37381 aatgatacca aggataatac gataccagcc aaaagcggta aagtcgtttt tcttcaaata
    37441 gcttagtaag aatttgatag ctaagacgga aaccacaaaa gatacgaatg ttcctacaag
    37501 aagaacaaca gtttcttcca ttttgaatga attcccttca gctaaatatt tgacgatctt
    37561 caagccgctt gcgccaaaca tgacaggaat ccctaagaaa aatgaaaact ctgtagcgac
    37621 aaaacgcgaa gcaccaatca agatagatcc taggatcgtt gcgccagaac gggatgttcc
    37681 gggaacaagt gccaagactt ggaataaccc aacaagaaga gctgcttggt acgtaaagtc
    37741 attcagagaa gtccaacgag gtgttttgtt tttgttgcgt ttttcgacaa taataaaggc
    37801 aataccgtaa acgatcaaaa caatggcaac tggtaagaac ttgtggaaat ttgcttctaa
    37861 ccaatcatcc aaaggcaaac caatgatgat ggctggtatg caagcaacga tcactttaaa
    37921 ccaaagtgtc cacgtatctt ttttttctac cgttgattta cttggagaaa atgggtttaa
    37981 cttgtgaaaa taaagaacga cgacagccaa gatcgcccct aattggataa cgacattgaa
    38041 catcgacata aattgtgaac tggcatccag tttgatgaat tcatctgcta ggatcaaatg
    38101 accagtacta ctgatgggca gccattccgt tattccttca ataataccga gaatcactgc
    38161 tttgataata tttgcaaaaa acataaattc cttccttccc aattaagata catcttatag
    38221 tataccgtta ttttttgaga aacccaatct gttttttaat attaagaaaa acttgagaaa
    38281 taaagcagat aagtacacga agagaaaaaa gaagacaaag ccctcgttca gactttgcct
    38341 tttatgattc tctggtaaaa attaaaatat ccccattata gaaacgagtc gcgactatgt
    38401 gttttcccgt tatcgatata agacgtatca ttgatgccgt ggatagaaaa tcgaccgttt
    38461 gcataagtga cctttgttac gctgccattt aaaagcccag cacgaacagg ctgtttagga
    38521 tcgatcagat taagcaaaaa ggcgatcgtc aaaccatgag atacgactaa aacatttcca
    38581 ccaccatttt tttcgcagtg atgggcgata tcttcaaatc ctgtccatac acggtctcgc
    38641 aatgtttcat aagtttccgc ccacccagcg gtatctaagt tgtatacagc attagcaatt
    38701 tgttcaaaag aaacttctgt ggtaaacatt tcatcatatg ttttaaaatt caatacacga
    38761 gggatcacac cccacatttc catgtcatat ccaccttcaa gagacccgaa acaccattct
    38821 ctaatccttt tttcaataaa ataaggaatt tcttttccct tagaatgttc tcctagaata
    38881 atacgggcag tttcgattgc acgaccactg tcactagaat aagcaaatga aaagtcgaca
    38941 ttttttaacc cgcgacctaa ataatgaatg ccttcttgtc cttgctttgt gaggggtgta
    39001 tcacaccagc cttgtactcg ttcaattgta ttgaacatcg tttttccgtg tcgtacgata
    39061 taaagtgtag tttctgtcat tttattctcc tcctaagtcg ttttaacgat tgaaaaaagg
    39121 atttgtcctt ttttcctgtt caatcgtcgt tgcatcgcca tgtcctggat agactgcaaa
    39181 ttcatcaggt aatacaaaca gctgggtccg aatactatga agtaattgtt ctaaattccc
    39241 tgtatacaga tcggttctac cgatacttcc tctaaacagg gcgtcgccag aaacgacaaa
    39301 atcatcaaaa ataaagctga cgcttcctat agaatgccct ggggtaggga cgacgcgaaa
    39361 agacatgttt cctaaagtat aagttttcat ttcaaattca tattctgcag gcgagacaat
    39421 aacatcagca atatcatcat gtctaccgag tccagaaaga ttcaaaatcg gatcacctag
    39481 ccatttttgc tctagtgggc tgacataaac tgggatttga taatacgcgc gtaaaggttc
    39541 cactgcaccg atatgatcgt agtgagtatg ggtaagcaaa atcgctaccg gctgttggtt
    39601 tgtttttttg atttcttgtt tgattcgttc cgcatcttct ccagggtcaa tgatcaaaag
    39661 tgcttcttcg ttgtagacca aataacagtt ttcttcaatc attcctgttt ttaatctttc
    39721 gatatgcatg tgcattcctc cttgatagcc aaaacttctt tcatccatta gtataacgca
    39781 ttcattgcta ggaaacaaaa acaggtactt atttctcttt acttatctga attatcagac
    39841 tatatgaaaa gtatttggat attctaaaga gaaggaaagt aagttttcat agcattttaa
    39901 tatgtccttg ttacattaga ttcatccaaa aatccaaatt ctatatccta tatccctgcc
    39961 taatcagcag ggtgtttttt tatgtttgga aagagagctc tttttgctaa actaggtttt
    40021 ttcaggtaat cttagagaga aagaggtggt ggaaatgatc aaacagtcaa aaatatctgt
    40081 gcggcactta gtagaattta ttttgcgacg tggaagtatc gacaatcgta aaaaaagtaa
    40141 ccatactgca ttagaaggag cgagaatcca ccgcaagctc caaaaagatg cgggagaaaa
    40201 ctatgaaaaa gaagtctttt taaaaacaac agttcaatta gatgaatatt acctgatcgt
    40261 tgaaggccgt gcagacggta ttttcaaaag agacggtatt tattatatag atgaaatcaa
    40321 aacctccgaa ccacgatttg aagatttaga accagaacaa gtggaacttt tctttcatca
    40381 agcaagggtg tatgcctata tctatagtca agaacatgat ttacaagaaa taaatctgca
    40441 attgacttac ttccagacgg cagaagaagt gatcacgaga aaggttgaac atcagacaag
    40501 agagcagtta gaaacttttt ttaaaaagct gactgaagat tataaagaat ggcttgtttt
    40561 tcaagaaaac tggcgcactg tacgcaatgc ctctttgatg gctttgaaat tcccccatga
    40621 tgagtatagg aaaggtcagc gtgaactagc ggtagctgct tataaaacga tccgtacgaa
    40681 acagaaattg tttgtagaag cacctaccgg aacaggaaaa acaatctcta cgttattccc
    40741 cgctttgaaa gctgtagggg aagaagaggg agaaaagatc ttttatctaa cggctaaaac
    40801 aataactaga caagtagctg aagatgctat gacagcatta aaagatacag gagctgaagt
    40861 aaaaagtgtc actttgacgg cgaaagacaa gatctgcttt ttagatgaga cgacctgtaa
    40921 tccagatcag tgcccatatg ccaatggtta ttacaatcgg attaatgaag gtttatggga
    40981 tcttttgaat catgagaatc agatcactcg tgaagtcatc gaaacttatg caagaaagca
    41041 tacactttgt cctttcgaac tatcattaga tgtcagtatc tggtgtgacg taatcattgg
    41101 ggattacaat tatttatttg atccaactgt ttatttaaga cgtttttttg aagatgaaaa
    41161 aaacgaggac tatctgtttt tgattgacga agcacataat ttggtgaatc gttcccgaga
    41221 gatgtattcg gcagaactat cttatgaaaa aacgaaacga agtaaggaag ctataccaaa
    41281 ggaattcaaa aaacttcatc gccgtttcaa caaactatta aaagaatttg attcgatccg
    41341 agaaatagca aaagaagatc attgggatta tcatcatcaa aaagcaccgg ctgaatcttt
    41401 agtaaaggca ggttatcaat taagcgaaaa aattaaagaa tggctggcag aatttcctga
    41461 gcacccatca caagaacaat tgcttcctta ttattttgat ttgcttcatt ttttgaaggt
    41521 cagtgaattt tatgatgatc actacgaaac aacagtcgaa aaaacgtatc gcgatctgat
    41581 agtaaaagag ttttgtattg atccaagctt gtttcttgag cagtcgttgg ataaaggaaa
    41641 aagcagtatt cttttttctg ctagtttttc accactctct tattatcagg aaacactggg
    41701 gggacaaaaa agtctggctt acaaactgcc gagtcctttc ccagaaggaa accagcaagt
    41761 tttcatcgca aattacctag agacgactta tcgaaaaagg gaacacagtc tggcaaaatt
    41821 agtagagacg attcatgctt ttgctgatgg aaaggtcggg aattatttcg ttttctttcc
    41881 ttcttatcaa tatttagatc ttgcggtgga agcattcaga cagcaatatc ccgacagcaa
    41941 tgtgctaatc caagaaacgg acatgaatga ggaagaacgc gaacgatttt taacaaaatt
    42001 tcaaacaaat cccgctgaaa cattgattgg attttgcgta cttggcggga ttttttcaga
    42061 aggaatcgat ttgagagggg accgattgat cggcagtatg atcgtaggtg taggtttgcc
    42121 gcagatgaat catgagcagg aactgatcaa atcatattat gatgagaagg aacaccttgg
    42181 atttgcttac gcttatcagc ttcctggaat gaacaaagtc ttgcaagctg gtggtcgagt
    42241 gattcgtggc atggcggatc aaggaatcgt tgtattggca gatcaacgat tttctagttt
    42301 ttcttatcga cgtttattcc cacaacattg gcaaacagca agagaagtac gaagtgttgg
    42361 ggaattggaa gaaggaatac agcgtttttg gttatcaaaa gaagcagtaa atcaaaaaaa
    42421 cgaggaatga cagacgtcag cctcgttttt tgattaacga tagttagtga attgcaactc
    42481 gattggtaag tcaagtttac gtaataaagc aatcacagct tgtagatcat cacggttctt
    42541 acctgtcact ctaatctgat cttcttggat ttgtgctttt actttcagtt tttcattttt
    42601 gatagctgta gtgattttct tcgcgttttc acgatcgatt ccgttgacca aatcggcagt
    42661 ttgtcgagct ttgccaccta gagcgtgttc tgattcggaa aaatggatat ttttgattgg
    42721 cacatttctt ttgatcagct taccaaataa tacatctttg atctgttcga ttttgaaatc
    42781 atcatcgctc aatacaacta gattgctgtt ctctaatttg atttctactg tagaatcctt
    42841 gaagtcaaag cgattggaca attcttttgt tgtttgctga atcgcatttt ttacttcttc
    42901 tgtattgact tctgatacaa tgtcgaagct agcatctttt gccataggaa ccctcctgat
    42961 atacatataa atatttacca tacatctatc ttatcatagg attagggcag attcttacct
    43021 gtttgctaaa attcttctca tttgagttcg aagtatttta ttttaataga taaactgtta
    43081 ttcttacatt tggaatacca aagtagggga gaggtgcttg tgagtaaaat tatgactgcg
    43141 tatcataaaa atgaaagatt aaagaaaata gtggttattt tgataaccgg attgaccgct
    43201 gcggtcgctt taaattattt tttgattcca gccaatgtct tttctgcagg gatgaatggg
    43261 atcgctcaaa tcatttcctc gcttttatat gaatggtttc atatcaagat agacacagga
    43321 acatttattt ttcttttgaa tatccctgtg tttgtattag ggtttctcaa agtaggaaaa
    43381 cgagcaacga tccttagttt tgtgaatgtt gtatgtgtat cggtgatgac gatgctgctt
    43441 ccagtcggac agatcactga taatattttg atgaatgccc tagtaggcgg tgttttatta
    43501 ggtcttggcg taggattatc tttaaaaatg ggctttacaa ctggtgggat ggatatcatc
    43561 tctcttgtac tttcacaaac aactggaaaa acagtcggaa actacatgct gattctaaat
    43621 ggtatcattg tagtcatcgc aggcttcttc tttaactggg aaagtgcctt gtatacgatt
    43681 atttccattt atgcaatgac tcaagtaatt gatgccatcc acacgagtca tcaaaaagta
    43741 acagcaatga tcgttaccgt caagccagaa gaggtaacag aagaactagc aaaacggatg
    43801 gtacgtggaa tgacattact gccgtctgtc ggtggctatt caaaaaaaca agggaacatg
    43861 atcatgatgg tcatcacccg ctacgaaatg tatgatcttg atcagatcgt tcatgaaatc
    43921 gacgacgatg catttatcaa tattttacca actcattcag tatttggccg gtttgcaaat
    43981 gaaaacgaac aaaaaatgta tcgaacaact ggcgtattgc cagaaataaa aaatcgttca
    44041 caaaaaaaat gaggaatcag gtttatttta gaataaattt aatctattac atctaataag
    44101 aacattttta taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa
    44161 aaaaagagta tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac
    44221 caagaacggt ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa
    44281 atggtgattg atcctgaatt aggaatcgac atcgtcaatt taggcttgat ttatgatata
    44341 gaattcaatc ctgaaaatgg agaaacagtc attaagatga ctctaacaac tatgggctgt
    44401 ccattagcag atattttaac agaatctatc catgacgcat taaaagaagt gccagaagta
    44461 acgaaagcag aagtcaaact agtctggtat ccagcatgga cgacagataa gatgagccgt
    44521 tatgcacgga ttgcgttggg gatacgatag aaatcttggc tgactagcta tgagaagcaa
    44581 ataggaaaaa caaaaaagcc ttttatcctt tctagaagaa aagagataaa aggtttcttt
    44641 gtttataaaa ttttgtctgc tatattataa gttaattaaa taaatgtgtt ctattgataa
    44701 aggacattca aatccctttc cttttggaat ccttcttttt tacatcagtt tgattttcgt
    44761 atagtattct ttctcatctt cacgataaat cgcagcccca tttttttgtt gtgctttaag
    44821 actgccaaga ttttttttat ttacagacaa aagtgcctca tcaatgccga ttttttttgc
    44881 ctcttgtagt actaatgcta atccttgtgt ggcatatcct tttttacgat aattagggga
    44941 gatgccgtaa ccaatatggc cagcgccgtt tcttaatgtt tcgttcagtt tatggcgcaa
    45001 attaaaaatt cctacatact catgttcatc actttctaaa ataaagacgg ttgcgggaac
    45061 gtaactctca ggaagatcga ttcctaaaga cgttttttcg cattttttta cataatctaa
    45121 aaattcagca aatgtatagc cataagcatt gttgataaat ccactctcat ttggtccaaa
    45181 gctagtttgt agctgataag ctttctttaa atctgcttcc catagtttga tcagtttcat
    45241 cgtattcacc tctttaagta accttattca tttgttttct actatagcac tattgttgta
    45301 aatagccatg cctattttta tttattgtgt catatcgtat taagaatcat cgaacaatac
    45361 aaatatgaat tacaataaaa aaattatttg aagttcgttc attcgttact tatgatctct
    45421 agaagaaaga aaacgtgaat tacttcagcg tctagaattc cagaaggata gtaatcaaca
    45481 gaagaaataa tcaatatcca aatgaaagaa gatagctgaa tcgagaaaaa gaggaaaaaa
    45541 gaaatgaaga aaaaaataaa atagttacta atcccagagt cgttcgttta acattgaaat
    45601 cgatcatatg gataaaacag attttttcac caaccctcaa tagatgatgg agttgctcgg
    45661 atgaattatt tgtgtagatg gcataaggga gggcgtacag aaatgaaaaa gaacaaaaat
    45721 atgttcttga caaaaaacac atgtaatcct aaactaaaag atgtgatgcg gctatggcgg
    45781 aattggcaga cgcgctagct tcaggcgcta gtgagggtga cctcgtggaa gttcgagtct
    45841 tcttagccgc ataatttgtt tttttataaa gagaaggtac acctggagcg atgagaaaga
    45901 aaataacata ttatctaaaa gaatatagga aaaattttaa acaacggaac cagattcaaa
    45961 taaatcaaaa aaaatttgtg tgaaggtggg acagtttaaa acctaataga ttgattcaaa
    46021 tagtagattt atagattctg atacaagaaa tactggcaaa cgatagttaa ggaataaaga
    46081 ctaaacttaa actcaaaaag aggtcatgcg aaagaataaa tcgcatgacc tcttactata
    46141 ttttgaatgt gtttaaacgt tcaaataatg aatcttagta ccaaccgttt gcttcccaga
    46201 atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt
    46261 cttggtttgc agcagagtag tcaccattta agtaagaaga atctaattgg taacggccga
    46321 tgtaacgtcc gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt
    46381 ttgcagagct tgtacttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg
    46441 tttcagcaac aggcgctgct tgtgtttccg tttgaaccgt ttcttgtaca acaggttgtt
    46501 cagcttgaac aggttcagct tggactggtg cttcttgaac ggctgcttga ggttcagcag
    46561 ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagtag atcaggttaa
    46621 tatcagaaat tgaatttgat tctgcaatgg cattgatcaa ggagttatca ccaacgtatt
    46681 tttgagaaat cgttgataga gtatcacctg attgaacggt ataagcttcg tccgcatgag
    46741 cagtcgttcc catgaagaat gcagcgccgg cagccaaagt tgttccaaaa agtaaagttt
    46801 taagtgatgt catgaaaaat ctcctttatt cgttaaaaag ttttatttgt tgtgttcaac
    46861 gaggaatact ttaacacgtg aaaatatttc ataggtaaaa gaagcatgac aatttatgac
    46921 aaatccacgt tttttgtaac agaatgatgt tcgctcgatg tgaaaaatga tgaggaattt
    46981 cacataatgc agaaataatg ggattagaac aggtgttctg atgagaaaag aaatgtataa
    47041 ataacttgaa agaaattgct tttgttcatc attttattta catattgcgt atattttttg
    47101 tatttttgct tgagatgtta caataaaaga aaaaggaggc ggttccaatg agtaaaagat
    47161 accagaacat catggtagca gttgacggat caagacaatc gatcaaagcg ttcgctgaag
    47221 cccttgactt agcaaaggac aatgaagccc acttatttat cgtgtcaatc atcaacaaag
    47281 ttgagctgac ccatagtgcc tatgcctttt caaaaatcta tgcagatgaa aaacaaagaa
    47341 cagaagtagc catgctgaaa aaaatcaatg atgccaaaga gtacggcatt gctgagatcc
    47401 acgcaatagt tgaaactgga gatccaagaa atttgattgc caatgtcatc cctggacaag
    47461 aaaatattga tttgatcgtt atgggagcga caggaaaagg tgctatacaa caagcattag
    47521 ttggatcgac cgcttcttat gtcgtatcac atgccccttg cagtgtgttg gtcgtgaagt
    47581 aatgagataa gggaggaata gaaaatggtt cgtcctcaag aagtccgtgc gccaaaagaa
    47641 aaaatagaaa ttttagcaat tattgaagac ggcacacaaa caaagaaggg atactcgatc
    47701 gctttagtaa agtggaaagg aaaaaaaggt gttgctctga gatgggatgg agataaccaa
    47761 caagataaag gattccctgt cacagctaac ggttatcatc cagcgtggtt tgttctgcct
    47821 gataaattta tggaattata cagctatgat tatgcaagag ccgcagcatc aatcaaagca
    47881 ttagataaat taacagaaga gcagaatgaa atggaggaaa aataaaaaag acgaaccaat
    47941 cagcctttag aaaactgatt agttcgagga gtaaaaatga aaaaaggtta tttgggatga
    48001 aactagaata atgcgtataa cttaaataaa acttaaagca ataaaatggc aagagggtga
    48061 ggcaaatgga agcaggaaaa gtattacagg aagtaaaagc actatgtgca gataaaaaat
    48121 atgaagaagc gaaaagcctg atcgaaaaga acaaagaaaa tttggaagaa aagtacccga
    48181 tcgcacaaga atttatagaa ttaaaacaag gaacgatcct tcaccgattc aaacattttt
    48241 ttggtataac agaatagaaa aaacgattaa agatcggcag aaatttgctg catctttaat
    48301 cgttttcttt ttaaacatgc gagggattca attctgattc ttatataaaa aatagatcca
    48361 aataataaga aaaattactg aaagcgtcaa ttcgatgatg tgtgttatta atacagacgt
    48421 tgtttcccaa tgcctaaaag aaaaaatttg ataagtaaaa tatacagcag gtaaagaggc
    48481 cagtaaaact gaaaacttct tttttgtcca ttttatgtaa tagaaaagaa tgcttatcat
    48541 aataaaaaaa ggaatataaa aaacaaattg accgatcgcg atcatactat tgcctccgat
    48601 aaataaagtc catgatacaa aataacactt tgtgcgaaat ggcgcaagca gttttgtgga
    48661 tttatccggt aaaatctgta tcgattttga aattagagga gaaacttgct ataatcaaag
    48721 tgaaagaagg agtgggattt tcatggatca tgtagtgtta gaaatcgatt tgcatttgat
    48781 caataatatt cgggtgattt actatgttgt aagtaacagt gtcgaacaac gtgtactgac
    48841 gaataaaata aatggtattt tagcaaagaa agacgttcac cgattcaata atggtgaagg
    48901 aagttattat agtatcccag tagaaaaaat tatttatacg acagtcaaag tcagagaaga
    48961 tctagatacc aaaaaagcat atgaacctat ttttacaact tattgattga cagcagatgg
    49021 aggaaaaaaa tgaatccata tgaacaactt attcaagatt tgatcgatgg aaaaatcgaa
    49081 aagattgatg taaaaagaga agaattaatg gcttttcgag aagcgtggct gaagttggag
    49141 aatcgtaaat atgtggtggg aaaagcctca ctgaacggca atgtgactta tcattacgat
    49201 ccgacacgtt tattttagta tgaagtattc agaaaagaac caaaggagga cgatgcagca
    49261 gcattgtcct cctttgattt tacaaaagtg aaagttttac ttatttgaat agatttttaa
    49321 atttgttttt gacactgtcg atatttacat cttttaaaag attttgggct ttctctttat
    49381 aaggtcctaa ttgttcttta tgttcttcga taaatcgttt tgcgccatcg aaattgccgt
    49441 tcttaatcat ttccttgact ttttgaaata attcttcttg attcattttg aacacatcct
    49501 ttgctatcaa catgattttt tacatggaca aaacgagaaa aactgattta tccacacgtc
    49561 aataatatca aataacgatc atttttacta aaaaaacact cgattctcgc ttctaaagta
    49621 ggagaaaaat ctccaacttg ggacggatgg aatttcttta actgacccat atactgaata
    49681 tataacaagg aggtagaaag atgaaagtga ttaaatattt gattggactg gcggcgatta
    49741 gtggtatttt gctatttgga ctgaaatatt atactgaagg aagttatgga gaaatccctg
    49801 gtatacttga tcagctaaat ccattagttg aaaaaggaga ggtctatgta aagacacaaa
    49861 aaccagagga agtaaatgaa tatggtacag caaggtatgt gcagaaagct gctgatgcca
    49921 atgggaaaga acgaacggtt gaatttaatg gtctttctgt gttaaaagaa aatcattatt
    49981 tgaaaattag caataaagga gcacacatag aaacgtatga ggaagttcct agagaaaaag
    50041 ttccggagaa agcattagca attattgggt aaatcagttt ttgtattaag aatacgcccg
    50101 attttattat tcataattta attatgtaaa ctaaatacaa gtagtaaaaa taatacaata
    50161 atccccctgc aaaaagattg ttcttttaca gggggttcat tttttcagca agcacatcaa
    50221 attgggatgg cagggttcga acctacaatt gcttgattca gagtcaagtg ccttaccagt
    50281 ttggctacat cccatttatt caacaaaata atcatagcac tttcctaaaa aaacgcaagt
    50341 tatttttctt gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac tagtttcttt
    50401 tctaatccag caggaagggc aatcagttct gctactgaca agacggtgtc caataactga
    50461 tattctttga caggttcatt cataatatga tagatgtttg ctaaacgttc aatatattca
    50521 aagcgagtat actctgttcg agcaattaga aatttcaata aatcggtggc tttctcgtac
    50581 tcttgatctg cgtaataaac aagagattga cgatacaatt tgatttccag ttcattgagg
    50641 gtagcaggtc tgcgacgctc gttccactca ttaatagaga ctttaccttg aacatttccc
    50701 ttgcagtcac ggacaaaaat aatcggaatt tcatttggat ccattttttt atgtgtgaat
    50761 ttttccatta tctacgctcc cttcaaaggt ttggtatctt gagtataagg gatttttgtt
    50821 tgaattacta gcttttctcc aaagttagca tctgtccacc cattgcagtg aaaatcgccg
    50881 aaaaatcgtt gatcggtact gctcgatttt ttacaccata gggatcgttg acataaatat
    50941 tttctttgtc atatccagtg actacacaag catgaacaag aggagtgact cttacttctc
    51001 cattatttgt ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa ttgatagtca
    51061 cagaagtcca gacaggattt cctttagata gtgctttttt gatatcatcg agttgagcat
    51121 cggtttttgg aaaagtgtga taatccgttt ggacgatttg ctgagcaagg gaggcaactg
    51181 ggggaacata gacgaccata gcccagtctc cgccactgat atcaccaaca aatccttcat
    51241 taggatcgcc atgagtccca tctgcattga aaacagggac gtaatccaat tgctcagcta
    51301 actggttttt agttgtttca taaccataaa aagataaaag catagaaagc gaagtgactt
    51361 cacagccatt ttctaaagaa ggagaatcaa actggctctc taatggaaca tctagtaaaa
    51421 cttctgcttt ctcttttgtc tttttcgttt tttgtgttga acttttttgt gcagtgttca
    51481 ttgtatgttc tggagaatgg atatgtaggt aataacttgt accgcttaat aaaagagcag
    51541 ctgtgccccc tagaaacaat agttttttga acgttttttt cttcatttga aatctccctt
    51601 ttcagctgtt tatctttcct attataaatg tatttttatt ggaaatggaa gtattatatt
    51661 ttaatatgta ggaaataatt agtttttaag gaatatgttt taattttttg aaaaaaattc
    51721 ttctaaatac aacagctgtt ttaattgtga aaacgaaaac agtacgctat aatgcctgta
    51781 aggttgattt ttaactgtaa ataaaaatca atgagttaaa tctccaaaga aatagcagtg
    51841 gtaatgtgga gaaaagttcc agcagtttag gaggattttg atgacggaag aaatgattaa
    51901 tttaggagaa caatatgcat gcaaaccaat tggttttaca aaaacggtca ttggtgaagt
    51961 tgtatcaaaa atgacaaact gcgccgtggt caaagttgcc caatgtgctg cagaggacca
    52021 agaattatta gatgaaaaag caagcatggt tgttgcaaaa tatgatacat ttgaataaag
    52081 aaaacaattg gacgtgcggc gtgctgcaag tccaatttct ttgagaaggt aaatcagtat
    52141 tcttttccta ttttctggag aagaaaaagc gaggcgagtt gtcatggtaa cattctttac
    52201 ggttctattg tttttattcg ttgttgtgat gtttttattt ctttggctgg ttcgaaaaga
    52261 aattatttat cggactgtgc gaaatcgatg ggtctacttg gtgataccct tcttagtgtc
    52321 actagtcatt tggtatacac tcatcagtca tcctacagca gatgaattgg caaaagggat
    52381 cttgtcagca atgattttta ttagtttttt gttggacagt agaggaatca cagaagaagg
    52441 acttgttttg aatagctttg acaaaaaagg tgttcctttt tctgagatca ataaaattgt
    52501 attatatcag ccaaaaggtt ctaaaatcgt aaaaatgaat tttttccgaa acggatggcg
    52561 aggaccgatg ctgaaatttt ctgcatcatt agaagaatta gttccatttt tgtcccaacg
    52621 cttaaacgat gaggcagaga ttgatattat gattgatcca gaataaaaac agaggttgcg
    52681 acaagatgtt tgtcacaacc tctgtttttt atttcaaata aaattatcca aatacgcgga
    52741 agcgatcggc atcgttcatg tattcttttt ccccagccgg tgtttctggt gaaccgaata
    52801 ctaattgcga acgtagtttc cagtttgaag ggatattcca ttcttttgca actgcttcgt
    52861 caataactgg gttgtaatgt tgcaagtttg cgcccaatcc tttatcaact aatgctaccc
    52921 aagtattagc tgttgcgatt ccgtttgatt gttctgacca atctgggaag ttgtctgcat
    52981 ataattcaaa ttgttcttgt agacctttta ccacgtcagt atctttgaag aataatactg
    53041 taccataacc atttttgaaa ccagctaatt tgttttgtgt atttgggaaa gcttctgctg
    53101 gagtcaatgg gcgaagtgct tcttcagtga tttcccaaag tttttcatgt gcatctccga
    53161 aaaggataac tgcgcgtgtt gattgagcat tgaaagcagt tgggctttct ttaattgctt
    53221 ctttgatcaa agttgttaat tcttcattag aaagtgaaac attacgtcct agatgataga
    53281 ttgaacggcg gtttttcaaa gtgtcagtaa aagttgtcat aataaaattt ctcctttgat
    53341 ttgcgttttg cattttttta attgattacg aaatgaagta tagcacctta caaaaagtaa
    53401 gtaaacagat atgctcacaa aaaataagcg aagttcaaaa aacaatttta ataagaaaaa
    53461 tcgttatgta aggattttct tagttatcgc tttcattgtt gtgttttagg aatttttgct
    53521 tatttgaaaa aaacagctga tatataatta ttaaatctct atcttctttt ttttatgata
    53581 ttgccattaa aaattacttg cttttataga aaattagttt atatttctta aataagtttt
    53641 ttagttaaat ttttcggtaa agatgtataa tattttctga aaatttaaag aattggggta
    53701 tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg attgtcaact
    53761 ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc attacttatc
    53821 tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc aacagcatta
    53881 gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg cggatgggta
    53941 tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt cattatgctt
    54001 ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc gatggcattg
    54061 attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg acatctttat
    54121 tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg tgtcaactta
    54181 ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa ctatcattta
    54241 gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat catccaagga
    54301 cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac tgaagaacgt
    54361 aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt attcggcggg
    54421 gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag tgtattgggg
    54481 attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt aactgctgaa
    54541 gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat tttctggtca
    54601 ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac acaatctacg
    54661 ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat tttcatcttt
    54721 attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca accatctaca
    54781 gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct attgatgttg
    54841 ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt gggaagtttc
    54901 ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt gacaacaaaa
    54961 ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc tgatgcttct
    55021 tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga atctgcttac
    55081 ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac tgtgaaagaa
    55141 ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac aggaattgct
    55201 tatttgcagc agttcctg
//
LOCUS       DAJSVF010000011        51206 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSVF010000011 DAJSVF010000000
VERSION     DAJSVF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.011"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(134..928)
                     /locus_tag="ON550_000626"
     CDS             complement(134..928)
                     /locus_tag="ON550_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCR3953734.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1574..4381
                     /locus_tag="ON550_000627"
     CDS             1574..4381
                     /locus_tag="ON550_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3953735.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            4572..5063
                     /locus_tag="ON550_000628"
     CDS             4572..5063
                     /locus_tag="ON550_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3953736.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5166..6155
                     /gene="manX"
                     /locus_tag="ON550_000629"
     CDS             5166..6155
                     /gene="manX"
                     /locus_tag="ON550_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR3953737.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6188..6991
                     /locus_tag="ON550_000630"
     CDS             6188..6991
                     /locus_tag="ON550_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR3953738.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7012..7926
                     /locus_tag="ON550_000631"
     CDS             7012..7926
                     /locus_tag="ON550_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR3953739.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8009..8383
                     /locus_tag="ON550_000632"
     CDS             8009..8383
                     /locus_tag="ON550_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR3953740.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8522..8767
                     /locus_tag="ON550_000633"
     CDS             8522..8767
                     /locus_tag="ON550_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3953741.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8939..10003
                     /locus_tag="ON550_000634"
     CDS             8939..10003
                     /locus_tag="ON550_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3953742.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            10249..10674
                     /locus_tag="ON550_000635"
     CDS             10249..10674
                     /locus_tag="ON550_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3953743.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10667..11116
                     /gene="nusB"
                     /locus_tag="ON550_000636"
     CDS             10667..11116
                     /gene="nusB"
                     /locus_tag="ON550_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR3953744.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            11213..12067
                     /locus_tag="ON550_000637"
     CDS             11213..12067
                     /locus_tag="ON550_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR3953745.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            12068..13405
                     /locus_tag="ON550_000638"
     CDS             12068..13405
                     /locus_tag="ON550_000638"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR3953746.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            13410..13634
                     /locus_tag="ON550_000639"
     CDS             13410..13634
                     /locus_tag="ON550_000639"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR3953747.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13636..14526
                     /locus_tag="ON550_000640"
     CDS             13636..14526
                     /locus_tag="ON550_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3953748.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14535..15353
                     /locus_tag="ON550_000641"
     CDS             14535..15353
                     /locus_tag="ON550_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR3953749.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15460..15909
                     /locus_tag="ON550_000642"
     CDS             15460..15909
                     /locus_tag="ON550_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3953750.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15927..17609
                     /gene="recN"
                     /locus_tag="ON550_000643"
     CDS             15927..17609
                     /gene="recN"
                     /locus_tag="ON550_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR3953751.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17664..17780)
                     /locus_tag="ON550_000644"
     CDS             complement(17664..17780)
                     /locus_tag="ON550_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR3953752.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17853..18809)
                     /locus_tag="ON550_000645"
     CDS             complement(17853..18809)
                     /locus_tag="ON550_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3953753.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            18980..19348
                     /locus_tag="ON550_000646"
     CDS             18980..19348
                     /locus_tag="ON550_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR3953754.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19518..19949
                     /gene="mraZ"
                     /locus_tag="ON550_000647"
     CDS             19518..19949
                     /gene="mraZ"
                     /locus_tag="ON550_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR3953755.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            19970..20929
                     /gene="rsmH"
                     /locus_tag="ON550_000648"
     CDS             19970..20929
                     /gene="rsmH"
                     /locus_tag="ON550_000648"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR3953756.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20934..21335
                     /gene="ftsL"
                     /locus_tag="ON550_000649"
     CDS             20934..21335
                     /gene="ftsL"
                     /locus_tag="ON550_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR3953757.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            21336..23528
                     /locus_tag="ON550_000650"
     CDS             21336..23528
                     /locus_tag="ON550_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3953758.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYPYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23554..24516
                     /locus_tag="ON550_000651"
     CDS             23554..24516
                     /locus_tag="ON550_000651"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR3953759.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24530..25891
                     /locus_tag="ON550_000652"
     CDS             24530..25891
                     /locus_tag="ON550_000652"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR3953760.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25917..27005
                     /gene="murG"
                     /locus_tag="ON550_000653"
     CDS             25917..27005
                     /gene="murG"
                     /locus_tag="ON550_000653"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR3953761.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            27020..28240
                     /locus_tag="ON550_000654"
     CDS             27020..28240
                     /locus_tag="ON550_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR3953762.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            28396..29721
                     /gene="ftsA"
                     /locus_tag="ON550_000655"
     CDS             28396..29721
                     /gene="ftsA"
                     /locus_tag="ON550_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR3953763.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29744..30985
                     /gene="ftsZ"
                     /locus_tag="ON550_000656"
     CDS             29744..30985
                     /gene="ftsZ"
                     /locus_tag="ON550_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR3953764.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            31002..31679
                     /locus_tag="ON550_000657"
     CDS             31002..31679
                     /locus_tag="ON550_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR3953765.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31693..32292
                     /locus_tag="ON550_000658"
     CDS             31693..32292
                     /locus_tag="ON550_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR3953766.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            32307..32594
                     /locus_tag="ON550_000659"
     CDS             32307..32594
                     /locus_tag="ON550_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR3953767.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32643..33425
                     /locus_tag="ON550_000660"
     CDS             32643..33425
                     /locus_tag="ON550_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR3953768.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            33446..34153
                     /locus_tag="ON550_000661"
     CDS             33446..34153
                     /locus_tag="ON550_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR3953769.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            34415..37213
                     /gene="ileS"
                     /locus_tag="ON550_000662"
     CDS             34415..37213
                     /gene="ileS"
                     /locus_tag="ON550_000662"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR3953770.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(37262..38788)
                     /locus_tag="ON550_000663"
     CDS             complement(37262..38788)
                     /locus_tag="ON550_000663"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR3953771.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38803..39450)
                     /locus_tag="ON550_000664"
     CDS             complement(38803..39450)
                     /locus_tag="ON550_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR3953772.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39634..39963)
                     /locus_tag="ON550_000665"
     CDS             complement(39634..39963)
                     /locus_tag="ON550_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR3953773.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            40140..40868
                     /gene="sfsA"
                     /locus_tag="ON550_000666"
     CDS             40140..40868
                     /gene="sfsA"
                     /locus_tag="ON550_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR3953774.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40884..41897
                     /locus_tag="ON550_000667"
     CDS             40884..41897
                     /locus_tag="ON550_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3953775.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41897..43174
                     /locus_tag="ON550_000668"
     CDS             41897..43174
                     /locus_tag="ON550_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3953776.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            43237..45939
                     /locus_tag="ON550_000669"
     CDS             43237..45939
                     /locus_tag="ON550_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3953777.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            46091..46408
                     /locus_tag="ON550_000670"
     CDS             46091..46408
                     /locus_tag="ON550_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3953778.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            46438..46758
                     /locus_tag="ON550_000671"
     CDS             46438..46758
                     /locus_tag="ON550_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3953779.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46866..48326
                     /locus_tag="ON550_000672"
     CDS             46866..48326
                     /locus_tag="ON550_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3953780.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            48394..48615
                     /locus_tag="ON550_000673"
     CDS             48394..48615
                     /locus_tag="ON550_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953781.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48646..48828
                     /locus_tag="ON550_000674"
     CDS             48646..48828
                     /locus_tag="ON550_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR3953782.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48828..49241
                     /locus_tag="ON550_000675"
     CDS             48828..49241
                     /locus_tag="ON550_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR3953783.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            49364..50545
                     /locus_tag="ON550_000676"
     CDS             49364..50545
                     /locus_tag="ON550_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953784.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(50616..50780)
                     /locus_tag="ON550_000677"
     CDS             complement(50616..50780)
                     /locus_tag="ON550_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953785.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            50934..51007
                     /locus_tag="ON550_000678"
     tRNA            50934..51007
                     /locus_tag="ON550_000678"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50968..50970,aa:Arg,seq:cct)
BASE COUNT        17022 a         8648 c        10947 g        14589 t
ORIGIN      
        1 aatttaaacc ataattgtgt ttttccttcc attaacatat aaagttcacc tcaaaaagag
       61 tatacgaaca aatgtttgta ttgtaaatag aaaaagatga gctaaattaa atgcccatct
      121 tctttgatag atattataaa tctaataatt cttgtttctt tttattaaat tcttcttctg
      181 taatgattcc atcatccaat agttctttat attttcttat ttctgaagct gaggaaattg
      241 ttgatgtatt ggaatttgta gcagaaatat ttcttctttt taaattataa ataatttcgt
      301 ttaaagaatc acttatccta tcaacagtat ctttatcaac tgcaacattc aatactttac
      361 caagagtatc aatagttaat gtacccaaca gcactcctct ttttttacta atattattta
      421 aattgtctaa tataatactt tgtacattat tgcctatcat tttttgttga gctacaatta
      481 aacgtttatt agttaatgcg tatgcataat tattgtcatg cttagttgat gagatataat
      541 tatgcaatcc tacaaagcag aagattacgt attcgtcact gttaagttga tctgatatta
      601 agttaaaatg atgagttgtc cattttttag tcattccttt accgtagccg tatttaagac
      661 aaaattctac catctcttca gtggttctca ttctatcatt acctatatca tgattgtttt
      721 gtgcagtatg atcagatatg tttaattttc ttttttctga tggatcctta tctaaatctt
      781 ctaaattcaa agatcttatt attttataag gagtagtcat accgtaacca gcttttttca
      841 tacaattgcc gcaaacaatt tgtttatctt tgattttata tgaagttaag ccaattttac
      901 ctccgcactt tgcacattgt ttactcacat tagttcctcc tagatatcag ctttctacta
      961 ctattaacat tctatcaata gtgaccatac tttgaccata ctttttagaa agaatagaaa
     1021 attccaagat atttaataat agaagaaaag cactaacatc atgggttcag aagaattgag
     1081 agcttatcag gaaacagtat aattgaagtg tctgtttatc cagtagctca agaagcttaa
     1141 ttgaatctta tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata
     1201 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
     1261 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
     1321 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
     1381 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
     1441 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
     1501 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
     1561 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
     1621 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
     1681 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
     1741 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
     1801 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
     1861 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
     1921 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
     1981 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
     2041 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
     2101 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
     2161 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     2221 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     2281 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     2341 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     2401 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     2461 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     2521 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     2581 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     2641 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     2701 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     2761 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     2821 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     2881 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     2941 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     3001 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     3061 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     3121 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     3181 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     3241 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     3301 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     3361 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     3421 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     3481 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     3541 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     3601 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     3661 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     3721 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     3781 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     3841 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     3901 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     3961 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     4021 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     4081 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     4141 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     4201 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     4261 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     4321 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     4381 aatacacaaa aatgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     4441 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     4501 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     4561 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     4621 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     4681 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     4741 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     4801 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     4861 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     4921 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     4981 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     5041 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     5101 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     5161 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     5221 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     5281 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     5341 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     5401 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     5461 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     5521 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     5581 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     5641 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     5701 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     5761 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     5821 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     5881 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     5941 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     6001 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     6061 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     6121 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     6181 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     6241 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     6301 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     6361 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     6421 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     6481 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     6541 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     6601 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     6661 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     6721 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     6781 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     6841 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     6901 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     6961 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     7021 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     7081 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     7141 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     7201 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     7261 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     7321 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
     7381 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
     7441 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
     7501 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
     7561 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
     7621 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
     7681 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
     7741 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
     7801 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
     7861 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
     7921 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
     7981 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
     8041 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga
     8101 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
     8161 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
     8221 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
     8281 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
     8341 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc
     8401 taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca
     8461 attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat
     8521 tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat
     8581 caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc
     8641 aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat
     8701 tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg
     8761 atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt
     8821 ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg
     8881 tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat
     8941 gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt
     9001 ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc
     9061 tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc
     9121 agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc
     9181 aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt
     9241 tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat
     9301 gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg
     9361 tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga
     9421 gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc
     9481 ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa
     9541 aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta
     9601 cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat
     9661 ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac
     9721 tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc
     9781 atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc
     9841 ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta
     9901 cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa
     9961 tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa
    10021 gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata
    10081 cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca
    10141 tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat
    10201 agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa
    10261 aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc
    10321 atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga
    10381 acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac
    10441 ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt
    10501 tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg
    10561 acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta
    10621 gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt
    10681 atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc
    10741 agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat
    10801 caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg
    10861 tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat
    10921 caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta
    10981 tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt
    11041 tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt
    11101 agaagctggg gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc
    11161 gggaaaattc tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga
    11221 attgattaac ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca
    11281 aaaattgaaa gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc
    11341 agccagccaa atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc
    11401 attggtagag cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa
    11461 atacaatcaa gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat
    11521 agatgaagaa aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc
    11581 gatgaattta ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg
    11641 catcatgaaa atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat
    11701 cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac
    11761 tgttactgtt gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat
    11821 tttagtcgta gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc
    11881 tgtagtgata gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa
    11941 atttgatgaa gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc
    12001 aatgactatc acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg
    12061 tgaatagatg gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa
    12121 atttgatgcc gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg
    12181 tttacgtgcc aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc
    12241 tattatgttc aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt
    12301 ccttgtcgtt gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga
    12361 gcacatggag cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa
    12421 gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg
    12481 aatcgcggtc cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa
    12541 aagaagatat ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc
    12601 agcggacaat gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat
    12661 gatcatcggt cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt
    12721 tgctcgggcg atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga
    12781 taccacgatc gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga
    12841 attagctgtt cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt
    12901 gcagcaggcg tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg
    12961 ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca
    13021 gttgaaacag cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac
    13081 gatcgcatta agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg
    13141 ccaagaaact gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca
    13201 acagcagcag cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat
    13261 catgggaaga ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga
    13321 gattcatcaa ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt
    13381 acaagagata gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc
    13441 aagaattaga aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc
    13501 tagattcgtt taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag
    13561 cagaagagac cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa
    13621 acgaggaaag ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa
    13681 aaagaaatct tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg
    13741 agttattcag tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt
    13801 gcttcctttg atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg
    13861 gtgcatacgt attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt
    13921 agaggaaaac ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat
    13981 ggtttgctta caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag
    14041 cttctgctgc ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa
    14101 gctgcagata tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat
    14161 gaaagaaaga cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag
    14221 caacctgaag aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt
    14281 cagataagag atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct
    14341 ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca
    14401 cgtgaggcgt tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag
    14461 gacgtttttt cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag
    14521 aaatgaggga aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat
    14581 ttgaaacaag agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa
    14641 acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag
    14701 gtaaaaaact tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat
    14761 ttgatctatc tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta
    14821 ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc
    14881 agctagcttg gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc
    14941 gttatgcaaa acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt
    15001 cttttatttc actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag
    15061 aagtgattgc tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg
    15121 gaattgttcg tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg
    15181 cacaaggtgg atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa
    15241 atatcgagtt cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta
    15301 ttaatatggc agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa
    15361 ctgtgaaaat caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta
    15421 tcattgatta agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat
    15481 cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa
    15541 atcctgaaaa gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa
    15601 ctgaaactga tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa
    15661 acaaacgaag atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt
    15721 gaccaaatgg aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca
    15781 aatttgattg ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat
    15841 gttttgatga ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat
    15901 tacctttaag ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga
    15961 ttattccgga attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg
    16021 gtgcgggtaa atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa
    16081 gcgattatat acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa
    16141 aacaagaagg attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc
    16201 tgattgttcg acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata
    16261 ttatcacatt agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa
    16321 acgaacatca ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag
    16381 atgtagcttt ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc
    16441 tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata
    16501 tgctccattt ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga
    16561 aattaaaaga agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg
    16621 caggttacgg agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg
    16681 tttcagaact gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata
    16741 tccaaagtgc ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt
    16801 tattggagtt agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac
    16861 agttgaaacg taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca
    16921 ccgaagagct agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac
    16981 tagagcaaaa agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa
    17041 aaatcgcaaa agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa
    17101 acactgagtt tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat
    17161 ttgaccaaat cgaattttac attacgacca atccaggaga gccactgaag cctttggtga
    17221 aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt
    17281 ctgagcaagg tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag
    17341 ttgcacaggc catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca
    17401 tcactcatct gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag
    17461 tgattgatgg acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag
    17521 aaattgcacg tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag
    17581 aactattgaa actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag
    17641 cagctgatag ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat
    17701 ccaatcgtta aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt
    17761 gtttgttttt tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac
    17821 aatctttttt ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa
    17881 taggtagtta cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc
    17941 ctttcagcaa taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata
    18001 gttaaaataa tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg
    18061 attgctgaaa aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc
    18121 attgcctgct ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct
    18181 tggaaaagtt tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct
    18241 ctgaaataaa cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg
    18301 gatacttgaa gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg
    18361 ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata
    18421 tcactttttg gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta
    18481 attgtgataa gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga
    18541 aaataaccgc taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt
    18601 gtcaactttg attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa
    18661 gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta
    18721 ggacgttcta cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc
    18781 tcattatcta atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt
    18841 ataacggata gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca
    18901 taaatttaaa taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat
    18961 aaaaaaggag tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta
    19021 cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg
    19081 ttttaaaata aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt
    19141 gtctagaaat acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat
    19201 gggaatttgt attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta
    19261 tttcaagatg acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat
    19321 cattgtgaac atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa
    19381 aaggcttttt gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg
    19441 atttcccaga aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga
    19501 ggaatggggg gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac
    19561 gtttaatcgt gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg
    19621 gattggatgg ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac
    19681 tgaacgaaat gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg
    19741 ctgccactga atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa
    19801 attatgcagc tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt
    19861 gggatgagac acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg
    19921 ctgaaagtat gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac
    19981 atttcaacac tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga
    20041 tggcgtttat gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca
    20101 actaagttct aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc
    20161 caagctgcgt ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt
    20221 cagagaatta gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga
    20281 tctaggggtt tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga
    20341 tgcaccgttg gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa
    20401 tgaatattcc taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc
    20461 caaacaaatt gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg
    20521 tgagcttgtc gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca
    20581 tcctgcaaaa agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga
    20641 agaggcctct ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat
    20701 cacattccat tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt
    20761 acaagatcta ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt
    20821 cattactcgt aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg
    20881 cagtgcaaag ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag
    20941 aattaaagaa aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac
    21001 aacaaatgcc tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac
    21061 gattaaagca gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg
    21121 ttgctttatg tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa
    21181 tcacgtcaat acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag
    21241 aaaaaaacga actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt
    21301 ctattaatga cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc
    21361 ggtttatgga aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta ggtatcattt
    21421 tatttgctac gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta
    21481 ttggaggtca tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa
    21541 gtgaagtagt aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag
    21601 aagatgcctc ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata
    21661 aaaaactgta tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc
    21721 tttctataga caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt
    21781 atcaagttga atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg
    21841 aagcggacat gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga
    21901 tgtatccaaa tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg
    21961 agaatggctt agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta
    22021 aagatggtaa gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg
    22081 cagaagaaga aaaagctgtc gacggacaag atatctacac gactttagac agcagactac
    22141 aaagttattt agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga
    22201 cagcagtcct gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt
    22261 tcaatcctga aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg
    22321 tacaagatag ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg
    22381 aagaaggaga attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg
    22441 ctacgatcaa tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt
    22501 catggtcaag taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata
    22561 actatttaca aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa
    22621 acggtgcttt acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg
    22681 taggtgtgac gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa
    22741 tgattcaacc tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa
    22801 ctcaaacaga agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat
    22861 atatgagaga tgttgtcgaa agcgaaaact acggtagtgc ttatggtgta tatagtgtac
    22921 ctggatacaa tgtatcggca aaaaccggaa cagcccaaat cgcttctgat acagggggat
    22981 atcaaacagg agatacagcc tacccgtatt cgattgtgga gatggttcct tctgaagatc
    23041 cagactatgt cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg
    23101 ctaaaatcgc aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt
    23161 cagatacaga aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg
    23221 acgttgcagc agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta
    23281 aaaaagtaca aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga
    23341 tcctatatac tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata
    23401 tcatgaagct ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg
    23461 tcaaacaaga acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag
    23521 aagaataaga agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc
    23581 attgtcagca gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa
    23641 atgaaaaagc aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc
    23701 ggaactccta caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg
    23761 gttggagctt ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg
    23821 ttatacggag taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg
    23881 ggactgaact cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt
    23941 gtttaccgtt ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta
    24001 ggaatcattt atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat
    24061 ctgacagatg gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac
    24121 ggtctaattg cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta
    24181 ggaggtttac tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat
    24241 gtcggttcat tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa
    24301 tggacattgc ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa
    24361 gttacttcct ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac
    24421 tttgaaatgt gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt
    24481 gtgacttcgt tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat
    24541 atcaacatat gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc
    24601 ggccaaatta ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga
    24661 cgaaaatccg gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca
    24721 tccaatcgaa ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta
    24781 cagtcatcct ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga
    24841 gctagcttac gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac
    24901 aacgacaaca acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg
    24961 tttagcgggt aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga
    25021 taaaattgtg atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca
    25081 tatagcggtg attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga
    25141 atatgtcaaa gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct
    25201 taactggaat cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc
    25261 tttctcgaca aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta
    25321 taaaaaagaa aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga
    25381 aaatgcacta gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg
    25441 agaaacgttg cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca
    25501 aggcagaaaa ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct
    25561 aagtggattt gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac
    25621 atttgatgaa ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac
    25681 aaaagaaaag ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga
    25741 ttctgtagaa caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt
    25801 attatcacca gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag
    25861 attcatggaa gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga
    25921 aaattttggt tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca
    25981 attatgtaaa gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc
    26041 tagaaaacaa aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat
    26101 ttaaacgaaa aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc
    26161 gtcaagcaaa aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat
    26221 atgtttccgg agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg
    26281 aacaaaacag tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg
    26341 ctctttcatt taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta
    26401 atcctcgagc acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt
    26461 tggatccaga aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca
    26521 atcaagcagt atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg
    26581 cttcaggaga tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata
    26641 ttagtgtcca gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg
    26701 tggggcgtgc tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt
    26761 tggttcccag cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc
    26821 acgcaggagc agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga
    26881 cagtcaacga aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag
    26941 aacaaggaat ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa
    27001 aataaaggcg gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc
    27061 ttctgatgaa aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca
    27121 agagatgaaa aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga
    27181 ttctgaagaa gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa
    27241 aatcgaattt tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt
    27301 tttgaaccgg ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc
    27361 tttgatcatc actgtattgg gtataccgct gatttttttg atctattatg tttctcctta
    27421 tagcaaatta caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat
    27481 ctctgataca aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac
    27541 ttatatcgat cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa
    27601 agggatgaat gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa
    27661 aaacaatcaa tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa
    27721 tcctacaaaa aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt
    27781 aacaaagcaa tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta
    27841 cacgccaaga gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt
    27901 tattgtaaat atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga
    27961 tatggatgaa aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag
    28021 cactcaagaa acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc
    28081 ggatcaaaca aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc
    28141 gtctagcaca aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga
    28201 taatccaagt acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta
    28261 agaatgtact ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta
    28321 ttaataagta tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag
    28381 gagggaatcc cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca
    28441 tctgttaaag ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga
    28501 aacgcaaaat cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa
    28561 gcgatacaac gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta
    28621 agtgtcggtt tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt
    28681 aacggagatt caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt
    28741 gttcgttcaa tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc
    28801 gatgggttcg agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac
    28861 ggtctgttgt ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat
    28921 gccggattaa tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt
    28981 tctgacggag aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca
    29041 acggctgtta tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa
    29101 tttgtcacta aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg
    29161 aaaataaact acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta
    29221 gatgtgattg gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt
    29281 tcagctcgaa tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca
    29341 ttagagttgc caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg
    29401 gacttggcac aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg
    29461 ctgagaaacc cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt
    29521 gaagtgtatc aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg
    29581 gtagttgaac aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc
    29641 tacgatgagc cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc
    29701 tttacaaata tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga
    29761 taataatatc aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa
    29821 tgctgtaaac cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac
    29881 agacgtgcaa gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata
    29941 tactcgtggt ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag
    30001 tgaacaatcc ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat
    30061 gggtggagga accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg
    30121 cgcattgact gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg
    30181 ttttgctgca gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc
    30241 aaataaccgt ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga
    30301 agcagataat gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg
    30361 ttacgttaac cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt
    30421 gatggggatc ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat
    30481 ctcctctcca ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac
    30541 aggcggtttg gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc
    30601 agcaactggc gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga
    30661 aatccgtgtg acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag
    30721 ccgaccagct agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga
    30781 tcaagctaaa ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg
    30841 tgaacaaaat gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga
    30901 atttgataca ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc
    30961 accgtttttc cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc
    31021 agaaaatcaa gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag
    31081 ttacattagt agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag
    31141 ctggcgttat gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt
    31201 tatttgattt tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa
    31261 aattgattgt aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag
    31321 aaatccagaa gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg
    31381 aggaatcaaa acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg
    31441 cctgcacaaa aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg
    31501 agcaaatcca taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag
    31561 gttatgccca tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg
    31621 cgattgaaga aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa
    31681 ggaggaacaa aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct
    31741 gatgaggaag agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca
    31801 actaaaaaaa atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat
    31861 gaaaaacaaa aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa
    31921 aagaaaaaag taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca
    31981 gcttcaagaa cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa
    32041 ccaagagctt attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc
    32101 ttagtgaatt ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca
    32161 ggaacagttt atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt
    32221 acaccgactg ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg
    32281 tttgatctgt aagaggagga actacgattt atactattgc actattactt catttacttc
    32341 aacgggcagc ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc
    32401 caggcgggta tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca
    32461 gtttgttcga tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa
    32521 tcctgatctt gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc
    32581 atttagttta ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa
    32641 ttttggatgc aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg
    32701 taggcgattg gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg
    32761 acccaagaca atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca
    32821 tgttttatgg cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt
    32881 acgaacctgt agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat
    32941 ttgccagcct aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct
    33001 cttactttgg cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg
    33061 tggatcattt tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac
    33121 caatcaaata cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag
    33181 ttagttccat gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt
    33241 ccaagcagct gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg
    33301 attttgtact tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc
    33361 aagaacttga ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta
    33421 aataaaataa aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac
    33481 attcccgaca aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt
    33541 tgttcgtgac tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca
    33601 ttctgaagaa aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat
    33661 cgtagctcaa gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat
    33721 cgttacttct gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca
    33781 tcaattaaca actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa
    33841 agcaagacag cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca
    33901 acgtatcagc ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga
    33961 aattttacaa ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt
    34021 agcaaaacag cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga
    34081 agcacctatg acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa
    34141 tgaaaacgag tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga
    34201 agttcggtaa gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt
    34261 agagtaagaa gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg
    34321 caactaaatt tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg
    34381 tgtccggaaa acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt
    34441 aggaaaaaca aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa
    34501 agaatgggaa gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag
    34561 ctttgttctt catgatggac ctccatatgc caatggtaat atccatttag gacattcatt
    34621 gaataaaatc agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc
    34681 atatgttcca ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg
    34741 cgtgaaacgt aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact
    34801 ttcccaagta gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga
    34861 acatccttat gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa
    34921 aatggctgaa aaaggctata tttacaaagg attgaaacca atttactggt ctccgtcaag
    34981 tgagtcttct ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt
    35041 tgtagctttc aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat
    35101 ttggacgaca acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt
    35161 tacgtatgta ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga
    35221 aacagtccaa tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca
    35281 agaaatggaa tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt
    35341 aggcgatcat gttactttag acgctggtac tggattagtc catactgctc caggacatgg
    35401 ggaagatgac tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc
    35461 aaaaggattg tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa
    35521 tcctatgatc actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac
    35581 tcatagttat cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca
    35641 atggtttgca tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga
    35701 gtgggttatc ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg
    35761 ggtaatttcc cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg
    35821 agaagcaatc atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg
    35881 atcaattatc tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc
    35941 aggttctcct aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc
    36001 tggttcttct catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat
    36061 gtatttagaa ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt
    36121 ggctatcaat ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg
    36181 tgaaggccgt aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa
    36241 acaatttggt gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt
    36301 tcgtgtttct atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac
    36361 gatgcgtttc ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta
    36421 caatgattta cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga
    36481 aatccgagaa gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa
    36541 cttcttgacg gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat
    36601 tgaagcagaa gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt
    36661 agctttgaca aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt
    36721 cctgaaagaa gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc
    36781 aaaccaagaa gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt
    36841 aaaagcattg gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact
    36901 aactgtttat ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc
    36961 acaattattg atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc
    37021 agataacgct atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac
    37081 ttgcgatcgc tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct
    37141 atgcggtcgt tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt
    37201 cgaagaaaag taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt
    37261 atcaatcgtt taattttcca cgttcacgat accagttatc tggttgccag atccattcga
    37321 atccgtcttt ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt
    37381 tgggaaaatc cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag
    37441 attgagcgac ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata
    37501 gtaatttttc gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca
    37561 atttgattgg ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa
    37621 atccttcagt tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac
    37681 atggttcatc gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga
    37741 tccgagtccc tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc
    37801 aacggaagtt atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat
    37861 ccactttgtc ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat
    37921 attgcccgcg aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca
    37981 atgctttgat cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg
    38041 caagtaatgc tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat
    38101 gatcgtagta gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat
    38161 tgtcgatata gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg
    38221 agatgttttg gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg
    38281 ggaatacttc tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg
    38341 gtttttctat gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat
    38401 gagaaacgat cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt
    38461 ttaattgata gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg
    38521 aatcatttac atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat
    38581 ctgaaggatt caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc
    38641 agggacggcg agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa
    38701 ggcggaacaa agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg
    38761 taaacaatac atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa
    38821 aatcgtgctg gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat
    38881 cggtccttca taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat
    38941 agtgactaag taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt
    39001 accgacagga taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt
    39061 aggaatcgat cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc
    39121 tgcaagatgt tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca
    39181 gctatagtct aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact
    39241 gctagctttt tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac
    39301 aagctcttct ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag
    39361 actagatacg atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat
    39421 tttcaaataa tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca
    39481 tcattagtaa gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag
    39541 gatttttaga cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt
    39601 ttataaagga tatatatata cattgggagg gatctatttt tcagataaca acttatatga
    39661 agtgtctgca gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc
    39721 atactgatca cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga
    39781 ctgaccgttg atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct
    39841 atcactaaag aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt
    39901 tttctttttc aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat
    39961 catttttttt atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt
    40021 cttctttatt tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt
    40081 ttttactgtt tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt
    40141 tgaagtatca gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac
    40201 attgtatgaa aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa
    40261 aagaggtcct gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga
    40321 aaaccgccta tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc
    40381 aattgcctaa tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat
    40441 taactggtcc atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata
    40501 tttatttaga gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg
    40561 aaaataaagc gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag
    40621 gagaattaac gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt
    40681 tcgaacatct tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg
    40741 ttcgacatgc gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag
    40801 atgaggtaac tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta
    40861 tacaatagaa agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta
    40921 ctcatcaatt tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt
    40981 ctagacattt ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg
    41041 caacagattt gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc
    41101 cgaatagtct tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag
    41161 ttgaaaagcc agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg
    41221 aacacaatgt gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga
    41281 ttgccgatta cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat
    41341 attcttcgcg ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt
    41401 tctcaggtgg tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt
    41461 ttggaatgcc aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc
    41521 ttggatgggg gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg
    41581 gtgattctta tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag
    41641 tcaatgcaat tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg
    41701 cggtaacagc tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg
    41761 ctcatccgaa agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa
    41821 atgtgaacca agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata
    41881 atgaggaaga tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa
    41941 atatgaggat aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact
    42001 aagtaataaa caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta
    42061 tctttggcct ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc
    42121 ttcttctcaa gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa
    42181 agagttgaaa gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt
    42241 gaaggaaaaa ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc
    42301 aaaaaaaata aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt
    42361 tgacgctggg aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca
    42421 gctagctttt ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg
    42481 gaaaacgttt gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca
    42541 ttacttttta cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat
    42601 agaagatttt catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga
    42661 aaaattgctg tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact
    42721 ccgaaaagca gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga
    42781 tgtggcggct agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc
    42841 gttaagtgaa gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg
    42901 aatagtaatt acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc
    42961 atcaaatata acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc
    43021 aaccaaagaa gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa
    43081 ggagcagaac ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa
    43141 aaaagagaga aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa
    43201 aacgggaaca aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc
    43261 ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta
    43321 actatcgatc gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt
    43381 gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat
    43441 gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat
    43501 tctttaaaag tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg
    43561 catacgatta tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat
    43621 cattttgcaa tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt
    43681 gcggattatg ctcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg
    43741 gcttacacag gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata
    43801 ttgtttttag atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc
    43861 attgataaag gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt
    43921 cagatcattg gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg
    43981 attcctatgg caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa
    44041 atcatctctc tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa
    44101 aaagaagcga ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa
    44161 cgagacttga aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa
    44221 aagctaatca ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc
    44281 aaggaaatac cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt
    44341 gaaccaggat ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat
    44401 aacgatattg aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta
    44461 gagaccctca tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact
    44521 aaaagttcga ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg
    44581 tatgatgtgg ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt
    44641 gctttgcatc tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca
    44701 gatttgaata acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca
    44761 acgatcattg aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt
    44821 atgtttcttt ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt
    44881 ctcatattga tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta
    44941 ttaggaacaa aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg
    45001 tatgaagaag tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc
    45061 ttgttaacag atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc
    45121 attcggacga aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg
    45181 gcaagtgttg gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg
    45241 atcgtacgtg atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta
    45301 atcgtgacat gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca
    45361 gtgattaatc aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc
    45421 aaaaaacaca tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt
    45481 gatgttgatt atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa
    45541 ctaaatattt taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca
    45601 cttgctggat cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag
    45661 acagttcagc agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga
    45721 atcgttatcc atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa
    45781 tttgagaaac tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca
    45841 aacttgatgg cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt
    45901 cttacacaaa tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta
    45961 cacactaaac atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt
    46021 cagctctttt ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg
    46081 agtgttaaaa atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag
    46141 tttattggtc acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt
    46201 tgcggtctct gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt
    46261 attaggtcct caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg
    46321 tattccatta gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct
    46381 tgcacaagca gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg
    46441 gaagatcaac aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag
    46501 agcgatgcaa tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg
    46561 aaaatcgctg atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg
    46621 acacaagaag cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat
    46681 cacttaatga catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt
    46741 cgcgtataca gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac
    46801 ggatttttta ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt
    46861 aaaaaatgaa cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa
    46921 tcggagcgca aaaacattta gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa
    46981 caatggcggg atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt
    47041 ttaacagtac ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg
    47101 tcatcatcgg aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct
    47161 ttgccttttc ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg
    47221 ggatcgttgg tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag
    47281 cattagatgt gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg
    47341 caacgattgc agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt
    47401 tgaatggaaa cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt
    47461 gtaaattgat gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat
    47521 ctaaagcatt tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca
    47581 attatatcgt aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata
    47641 ttgcagaacc attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat
    47701 ttgttcaagt attctggttc tttggtattc atggaccaaa tgtattggca ccagtgctag
    47761 agggaatctg gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg
    47821 gaaaaactgg tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg
    47881 tccgcggatc gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac
    47941 tgatagctat cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca
    48001 ttgggcctgg tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga
    48061 atgcaatcat gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga
    48121 cagctactta tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc
    48181 cgttcttatt gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag
    48241 tatgtatggt cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg
    48301 accctgccgc aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg
    48361 ttcctcataa tgaattttcg aggaggaact gaaatgatta caattgccag tgcaatgcaa
    48421 caagatgaaa tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt
    48481 aaagaaaaac aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg
    48541 gctaaaaaag caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa
    48601 tcaacagctg tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt
    48661 ttatttttat gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa
    48721 aggtatgatt tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc
    48781 ttcatgaagg ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga
    48841 aaaaattaaa tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg
    48901 atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg
    48961 tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc
    49021 agtcttataa gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca
    49081 aaccattgga cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca
    49141 tgcttcaaaa gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac
    49201 gtttcaaaaa aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact
    49261 atttagttat tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt
    49321 cctgcgaatc ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag
    49381 agagctaggt gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg
    49441 ggcagataca cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta
    49501 cggatttttt gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt
    49561 gaatttttac ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga
    49621 agcaagggga caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga
    49681 tcgtaaagta agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg
    49741 ctatgaacaa tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat
    49801 caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag
    49861 aagaatttat gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg
    49921 gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa
    49981 tgaggaagca acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga
    50041 gtggggattt ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa
    50101 tggtgctttt gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct
    50161 attagaaaat ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga
    50221 tatttctttg tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca
    50281 aatagaagaa gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa
    50341 tggaaaaaat catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat
    50401 ccaacatatc acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga
    50461 aagaatacag gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca
    50521 gccaattctt aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac
    50581 ccttttgttt ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta
    50641 aatttgaatt ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct
    50701 tctttcgtaa tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt
    50761 tcttttggct tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt
    50821 ttatcatctg ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag
    50881 cagataagct tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg
    50941 tagctcagta ggatagagca atcgcctcct aagcgatagg tcgctggttc aattccagtc
    51001 aggaacgtat aaataacccc tgtatccttt gcggtacagg ggtttgtttt gtatttgtgc
    51061 caaaaatgtg ccaaactagt tatcattttt atataaacta tcgaacacag tattgatttt
    51121 ttctttatcc tgatcttcaa gctctcgaac aatatgaaga taagtagaca ttgtagtttc
    51181 taaactgcta tgtcctaaac gtttcg
//
LOCUS       DAJSVF010000012        50884 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSVF010000012 DAJSVF010000000
VERSION     DAJSVF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.012"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(718..3867)
                     /locus_tag="ON550_000679"
     CDS             complement(718..3867)
                     /locus_tag="ON550_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR3953786.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3881..5125)
                     /locus_tag="ON550_000680"
     CDS             complement(3881..5125)
                     /locus_tag="ON550_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3953787.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5118..6710)
                     /locus_tag="ON550_000681"
     CDS             complement(5118..6710)
                     /locus_tag="ON550_000681"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3953788.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7221..7946)
                     /locus_tag="ON550_000682"
     CDS             complement(7221..7946)
                     /locus_tag="ON550_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953789.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8100..8426)
                     /locus_tag="ON550_000683"
     CDS             complement(8100..8426)
                     /locus_tag="ON550_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953790.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8463..8828)
                     /locus_tag="ON550_000684"
     CDS             complement(8463..8828)
                     /locus_tag="ON550_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR3953791.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8865..9080)
                     /locus_tag="ON550_000685"
     CDS             complement(8865..9080)
                     /locus_tag="ON550_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3953792.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9209..9544
                     /locus_tag="ON550_000686"
     CDS             9209..9544
                     /locus_tag="ON550_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3953793.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9620..11617)
                     /locus_tag="ON550_000687"
     CDS             complement(9620..11617)
                     /locus_tag="ON550_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953794.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11715..11990)
                     /locus_tag="ON550_000688"
     CDS             complement(11715..11990)
                     /locus_tag="ON550_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953795.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12222..12956
                     /locus_tag="ON550_000689"
     CDS             12222..12956
                     /locus_tag="ON550_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3953796.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12995..14137
                     /locus_tag="ON550_000690"
     CDS             12995..14137
                     /locus_tag="ON550_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3953797.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14232..15623)
                     /gene="sufB"
                     /locus_tag="ON550_000691"
     CDS             complement(14232..15623)
                     /gene="sufB"
                     /locus_tag="ON550_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR3953798.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15628..16098)
                     /locus_tag="ON550_000692"
     CDS             complement(15628..16098)
                     /locus_tag="ON550_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR3953799.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(16085..17320)
                     /locus_tag="ON550_000693"
     CDS             complement(16085..17320)
                     /locus_tag="ON550_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3953800.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(17317..18603)
                     /gene="sufD"
                     /locus_tag="ON550_000694"
     CDS             complement(17317..18603)
                     /gene="sufD"
                     /locus_tag="ON550_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR3953801.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18616..19389)
                     /gene="sufC"
                     /locus_tag="ON550_000695"
     CDS             complement(18616..19389)
                     /gene="sufC"
                     /locus_tag="ON550_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR3953802.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19586..20434)
                     /locus_tag="ON550_000696"
     CDS             complement(19586..20434)
                     /locus_tag="ON550_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3953803.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20458..21144)
                     /locus_tag="ON550_000697"
     CDS             complement(20458..21144)
                     /locus_tag="ON550_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953804.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(21137..22171)
                     /locus_tag="ON550_000698"
     CDS             complement(21137..22171)
                     /locus_tag="ON550_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3953805.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(22234..22490)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22566..22904)
                     /locus_tag="ON550_000699"
     CDS             complement(22566..22904)
                     /locus_tag="ON550_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR3953806.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22906..23262)
                     /locus_tag="ON550_000700"
     CDS             complement(22906..23262)
                     /locus_tag="ON550_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR3953807.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(23268..24455)
                     /locus_tag="ON550_000701"
     CDS             complement(23268..24455)
                     /locus_tag="ON550_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3953808.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24646..25491)
                     /locus_tag="ON550_000702"
     CDS             complement(24646..25491)
                     /locus_tag="ON550_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3953809.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25725..26690
                     /locus_tag="ON550_000703"
     CDS             25725..26690
                     /locus_tag="ON550_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR3953810.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            27067..28110
                     /locus_tag="ON550_000704"
     CDS             27067..28110
                     /locus_tag="ON550_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR3953811.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(28172..28636)
                     /locus_tag="ON550_000705"
     CDS             complement(28172..28636)
                     /locus_tag="ON550_000705"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR3953812.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28882..29829
                     /locus_tag="ON550_000706"
     CDS             28882..29829
                     /locus_tag="ON550_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3953813.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(30040..32112)
                     /locus_tag="ON550_000707"
     CDS             complement(30040..32112)
                     /locus_tag="ON550_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3953814.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(32045..32422)
                     /locus_tag="ON550_000708"
     CDS             complement(32045..32422)
                     /locus_tag="ON550_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR3953815.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(32551..33129)
                     /locus_tag="ON550_000709"
     CDS             complement(32551..33129)
                     /locus_tag="ON550_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3953816.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            33261..34553
                     /locus_tag="ON550_000710"
     CDS             33261..34553
                     /locus_tag="ON550_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3953817.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(34613..35731)
                     /locus_tag="ON550_000711"
     CDS             complement(34613..35731)
                     /locus_tag="ON550_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR3953818.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35745..37166)
                     /gene="arcD"
                     /locus_tag="ON550_000712"
     CDS             complement(35745..37166)
                     /gene="arcD"
                     /locus_tag="ON550_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR3953819.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(37412..38533)
                     /gene="mnmA"
                     /locus_tag="ON550_000713"
     CDS             complement(37412..38533)
                     /gene="mnmA"
                     /locus_tag="ON550_000713"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR3953820.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38937..39536
                     /locus_tag="ON550_000714"
     CDS             38937..39536
                     /locus_tag="ON550_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR3953821.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(39674..40960)
                     /gene="efmA"
                     /locus_tag="ON550_000715"
     CDS             complement(39674..40960)
                     /gene="efmA"
                     /locus_tag="ON550_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR3953822.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(41231..41575)
                     /locus_tag="ON550_000716"
     CDS             complement(41231..41575)
                     /locus_tag="ON550_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR3953823.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(41712..42857)
                     /locus_tag="ON550_000717"
     CDS             complement(41712..42857)
                     /locus_tag="ON550_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3953824.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(43037..44011)
                     /locus_tag="ON550_000718"
     CDS             complement(43037..44011)
                     /locus_tag="ON550_000718"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3953825.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(44091..44966)
                     /locus_tag="ON550_000719"
     CDS             complement(44091..44966)
                     /locus_tag="ON550_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR3953826.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            45096..47003
                     /locus_tag="ON550_000720"
     CDS             45096..47003
                     /locus_tag="ON550_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR3953827.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(47544..50822)
                     /locus_tag="ON550_000721"
     CDS             complement(47544..50822)
                     /locus_tag="ON550_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR3953828.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT        14445 a        10616 c         8928 g        16895 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccctcc ctaaacaaac atcttttgta
     3901 agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtgt
     3961 cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc ctagtaaaag
     4021 gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt ccttcagtgt
     4081 tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg aactcattat
     4141 ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc gtcataccaa
     4201 gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg aattcatttg
     4261 gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca tcaaactgtt
     4321 ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag tctaaatctt
     4381 tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt tcccaatctt
     4441 ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg aacatttttt
     4501 gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga agagcgatag
     4561 tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat attggaaatt
     4621 gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct gaacctggat
     4681 tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata aaatattctg
     4741 aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat acacccggtt
     4801 ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc atgataaaat
     4861 cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta attttatatc
     4921 ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa ttgctattag
     4981 atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact ccatcttttt
     5041 tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct gggaaacgaa
     5101 tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg taaccacgct
     5161 tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt ttctagcact
     5221 tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc aattggttct
     5281 tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt ttctttgatt
     5341 tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc ataggtttcg
     5401 aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt gacaaattct
     5461 cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa tacaatcacc
     5521 gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac agcataaata
     5581 ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg gaataaaact
     5641 ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa accatgaaga
     5701 agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt aaaccgagaa
     5761 tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt cattactact
     5821 gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc cccattgcgg
     5881 agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc tagattgtat
     5941 gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt taagtagtta
     6001 cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact aaagaatcgt
     6061 cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat atgaggcgtg
     6121 taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag aaattcataa
     6181 gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa tttcttaatg
     6241 acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt tgattggaga
     6301 tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg cacaaaggct
     6361 ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa gacgttgaaa
     6421 agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat cagatcttct
     6481 tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct taccgtatca
     6541 tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg gtcggataaa
     6601 tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc cattttgcta
     6661 cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa aaagatacac
     6721 tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc ccttttagaa
     6781 aaagcaaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
     6841 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
     6901 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
     6961 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
     7021 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
     7081 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
     7141 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
     7201 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
     7261 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
     7321 ggaatatctt taccaacttt ctctgtcact gcattactga tacctgttag attagctaat
     7381 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
     7441 ttatcttcat ttacaagttt ccaagtaggt ggtatagctg tgtatgtttc tacaaatgaa
     7501 ttaagttttt cattaagtga cttctctttt atatttccat ctaaaaactc tgtatatatg
     7561 gcttgagcta tactaaaaca aagctggcct tgttctaata attttacatc attagttagt
     7621 aaagctgtgt tcacgttatc aatcgcttca agaagagtat ctttggtgtc cctaacagct
     7681 ttaggtgtgc cttctggaat ttccctactt gatacctcgt ccattttatc ttccataaac
     7741 cccttaaaat catatctttc tcccaaatcc atagaagttt ttgagaaagc tttcatttct
     7801 gagtctattt cttcaataat atttaacatg tctgatacat attgtttagg ctgattcgtt
     7861 tttaatctat atgtggtaaa tatctcaatt cctccaatta tcaataaaat tgccaaacaa
     7921 atccataata attttttctt tttcattttt cctcctatac actgctgtta taacagtgac
     7981 taatgtaaag attttcccag caatcatcag actattattt acagggaatc aaaaaaactc
     8041 cttagtaagc ttacaccttt aggagttcct gtttgaacgg tatgcataat acatttaaat
     8101 caatttaaca ttaacacctc agtatatggt atctctacat atacacggtc tttttttgga
     8161 tagtactcga catttatatt gatagcactt ccattgtcta acgtagcttt agcccggtac
     8221 gtgttatcac cattatcatg agtgatcgtc acatcatcac atgtccgatt taagttatac
     8281 tgttcttgta aaatctgagt taccaatggt acagatgact tctctagtcc agatgaaccc
     8341 gtcattcttg aaaaaattat acctataata gccaatacaa cgatacctat aatgacaaac
     8401 ttcttattca tatttacttt attcaacttt ttatctccta tctccaaaat gattttctct
     8461 catcagtaac tcattgacat ccccaatagt acaccaaaga tggctccagc tacccctgct
     8521 atcatcaaaa tcgtacccat ttgttcatct ttctctcgat ataagtaacc cataataaca
     8581 gcaacagcac caaataccac aggaataaat gctaatgata ctaccataga aatccaacca
     8641 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     8701 attgtctgct cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt
     8761 aatgctccgc aaaactggca atacttactt tcatttgtta tttctttacc acattttcta
     8821 caatacatgt cttctcctcc taaaatcatt caattgctta ttagctaact gtttacttta
     8881 ttaaatacat cagaaaacag ctcatttatt ggtacactta atatctcaga aagcattttc
     8941 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     9001 acattcattt ttttggctaa ctcagtttgg gaaagtcctt tttcttttct gtatcggctg
     9061 agattactta cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg
     9121 tatttcaact cataaaatat ataaatactt caaatgtcat tacaaataca acaacttgtt
     9181 gtactctact gagaaggggg gtgtattaat gaaacagaag caatatgcaa aggtcattag
     9241 agaaaagcga aaagcactcg ggtggacaca aaaagaatta gctaaaaaaa tcttctctac
     9301 acaacaggca gtagcaagat gggaaaattc tgtaacagaa cctaacctcg atagcttaac
     9361 agctctttct agagccctag gaacccctgt aagccatttt ctagacaatg ttgacataga
     9421 ttatgaggaa gagtttttgg ctctctatcg ctctcttagt actgaagatg caattcgaac
     9481 aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga
     9541 ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga aataagatta
     9601 agagctatgg ataacctgtc tagtttaata taattgggtc tcttctattc cttactctcc
     9661 gtccatcatc tcttgttaca cttgtaaatt gataaatagg aactaattca actcctgata
     9721 atatcaattc ttttattact tcatctatcc ctgccccttt cgctagatat actaaaaaac
     9781 tactgcttgc tttcacattg tatagtttct taatatcctt agaagctcgt tcaagagtga
     9841 atccttgttt aacaaagtag cctctgtttt ctattaaagc atttttcacg ctgttatccc
     9901 tgacatttcg taggcgttca caaacagtgt taataagttc tttttctgtc attagatatg
     9961 tcccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc aattgttttt
    10021 ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct ttttcgttaa
    10081 aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat ttttccagaa
    10141 gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa acgtctaatc
    10201 gatagacatt gcttctctta tgtttaaagt ttcctaacgc tttctcttcc tttaacctat
    10261 ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt atcaatccta
    10321 taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt ttaccatagt
    10381 aagaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac
    10441 tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta
    10501 gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta
    10561 cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata
    10621 gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa
    10681 aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac
    10741 tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac
    10801 ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag
    10861 acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt
    10921 cattttttat caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat
    10981 cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta
    11041 acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc
    11101 tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa
    11161 cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca atcttgtatt
    11221 tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta
    11281 aattgataga taaaaccttt tctatgctta agtatctact atcatcttta ctcaaatcta
    11341 ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt actctggttt
    11401 ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata aatgattgtg
    11461 atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta tctaaacacc
    11521 tatctcttac atcagttttt tctttttttg taactggctt ctctaataat ttttctttat
    11581 ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc ttataaaatt
    11641 ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta tatgttttag
    11701 tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta
    11761 aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta
    11821 atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga
    11881 gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact
    11941 tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct
    12001 cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat
    12061 aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat
    12121 acacataata cctcgataaa aaaggggtgt caatagtttt gtaaaattta tttacaacac
    12181 cccttttttg aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa
    12241 taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta
    12301 aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa
    12361 agactatcta cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa
    12421 ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata
    12481 ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca tttccagata
    12541 aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat
    12601 ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt
    12661 gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat
    12721 tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag
    12781 aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt
    12841 atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt
    12901 catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt
    12961 aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa agtacaaaaa
    13021 gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg
    13081 tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta
    13141 tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt
    13201 tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat
    13261 cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact
    13321 atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga
    13381 aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca
    13441 atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa
    13501 aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt
    13561 tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag
    13621 tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa
    13681 taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc
    13741 ttctagacga gttatctctt tagatactga gactctttct atcctcaaca actggaaatt
    13801 acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt
    13861 ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata
    13921 tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc
    13981 tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga
    14041 aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac
    14101 aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca
    14161 tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg gttctagctc
    14221 ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg attgagttcg
    14281 actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat gacgatcatt
    14341 tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg ttcttctgag
    14401 atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat ctcattgaat
    14461 gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc gatatgagag
    14521 atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac ttctccgccg
    14581 tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt gtggatcatt
    14641 tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag catggtgcct
    14701 cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt tgcacctagg
    14761 tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt ggttactaag
    14821 ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc atcttttctt
    14881 gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg tgccgtacaa
    14941 ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg ttcgaattgc
    15001 cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac tttgacgcct
    15061 tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc tgctaatttg
    15121 ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg gtattctttc
    15181 aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc tttcatatta
    15241 tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc tcgttcagct
    15301 tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc ttcccagtca
    15361 cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc gatgtctgaa
    15421 aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga tttcagacgg
    15481 aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat cacttcttct
    15541 gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc gaatttatat
    15601 tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat gatggaaagc
    15661 tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg tcgcacattt
    15721 gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta attggtcggg
    15781 atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat gttctgcttc
    15841 ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg tactgatgga
    15901 gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat cgacttgcaa
    15961 ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg agtcagtcaa
    16021 gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt ataaattgtc
    16081 taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct tcaactaaac
    16141 ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc gctgatactt
    16201 caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct accccttcca
    16261 tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat gcaacgactc
    16321 ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt gcttgtaatt
    16381 ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc ataccgagtt
    16441 tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt ggtgtccctg
    16501 cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca aaatcgatca
    16561 tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta ccatataaaa
    16621 caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa tcacagtcta
    16681 gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact acgatgaccc
    16741 cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca cctaagacat
    16801 tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc gctgcatcct
    16861 ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca cctgttcgtt
    16921 ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag agcactatct
    16981 cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc aagcttgtcg
    17041 tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac gcccgtactt
    17101 tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg acaccgcgat
    17161 gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact tgttttggct
    17221 tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg tcttgaaata
    17281 agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact tcccttcgat
    17341 tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga cagaaccgag
    17401 gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac tcatcagata
    17461 gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag ttacttcgtt
    17521 ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa gcatcaagac
    17581 acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc cgataccatt
    17641 aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc cgatcgtatg
    17701 aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg agctgattgc
    17761 cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa agtcagcgat
    17821 cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc gtaaaatgtg
    17881 cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg ctgaatattt
    17941 gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg attttgctac
    18001 ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt catctgccac
    18061 gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga ataaggactc
    18121 gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc tgttcataaa
    18181 agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt tcatataata
    18241 ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa aaatgactcc
    18301 tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt gttggacaag
    18361 cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac tgatggtttg
    18421 tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga tatgcggcaa
    18481 tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc aatcaggttc
    18541 gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt ttttttcttt
    18601 cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt ctttactgat
    18661 accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg tcatgactac
    18721 tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata atcgttgata
    18781 gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc ctttagcaac
    18841 gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg caaatgtagg
    18901 ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag aaaaaccttc
    18961 gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt tttcatctaa
    19021 ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg cattgatcgc
    19081 tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt gcatagctaa
    19141 aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt tcccgtcaaa
    19201 caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg cagataaggt
    19261 agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt tcattgttag
    19321 gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt ttatttctaa
    19381 gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta gactaattga
    19441 gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat tattaaacaa
    19501 ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat tacagactta
    19561 tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt ttaacagagc
    19621 cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact tcaatcagtt
    19681 ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc acgtaaggag
    19741 agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg ccgtaagcga
    19801 agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa gctaaaagtg
    19861 atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata tcatcgaatg
    19921 ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct tgtagaattg
    19981 tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca ccatctttta
    20041 attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg tggacagcac
    20101 cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa ggaatatgct
    20161 ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat acatagtcat
    20221 cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc acatgttcca
    20281 aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg tctgctttat
    20341 ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca gtcagcaaaa
    20401 gcgctgctgc aaatccaaaa attttctttt tcatatccta catcccccta tatcctatta
    20461 tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa atacgattag
    20521 taaaataatt aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt agccttcctg
    20581 ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag taaatccgat
    20641 catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt caggtatcaa
    20701 gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt ccagtacacc
    20761 agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg ctgcggataa
    20821 aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg ctttagtaaa
    20881 tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat tactgatgat
    20941 cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa tagagtaaag
    21001 gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta atgtcatata
    21061 tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa aatctagata
    21121 tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct tgttctttga
    21181 tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca gtcaatgaac
    21241 caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca tcgatgttat
    21301 agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta aaggtcaaag
    21361 ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct gtcgaatcag
    21421 cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc ggttgtctga
    21481 agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca gcgactttgt
    21541 tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc aaattcaatt
    21601 tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg tccaatgcgc
    21661 tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt gcgataccga
    21721 cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt ccttctaacc
    21781 cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt acgccagcta
    21841 attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg ttgaaatgct
    21901 ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt aattttgtaa
    21961 tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca ttcaggagac
    22021 ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata tccccttttt
    22081 caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca ttgaatgtct
    22141 ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct tataattttt
    22201 caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt gaacttccgt
    22261 gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac ttggacttag
    22321 cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt gcttacgctc
    22381 gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt cagcaaacag
    22441 ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc gtttcatata
    22501 ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca agtgattttc
    22561 tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa gatccacgca
    22621 ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc attaacagaa
    22681 gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc ttcaacttcg
    22741 atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc aaacgtgatg
    22801 tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt tccattaaat
    22861 aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata tcagttctcc
    22921 ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta agatcagtgg
    22981 tcgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag ccgtaaactc
    23041 gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt tgaacaggcg
    23101 acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct tcggtacttc
    23161 tttgatcata tcaacggttt gataactaac gccatgctgg tctaaccacg ctttggcttt
    23221 tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta cctccttgaa
    23281 gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat accgatcatg
    23341 ttccctaaaa tcgaggatcc tccctgacta atgaaaggca atgggatccc tgttaaagga
    23401 agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat catgatcaaa
    23461 ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg aatcatacga
    23521 tagattaaaa taaaatacaa gaaaatgacg aatgcaccac cgataaagcc gaagttttca
    23581 ccgataacgg taaaaatcat atcggactca cgaacaggca catagatatt gcttacgttg
    23641 aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc cggctgataa
    23701 ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc aaactgataa
    23761 gatttgaatc caactttata aagcaagtct cttccgcctt cagtcgtaac caagaagatc
    23821 gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg ccaagagatt
    23881 ccagacatca ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt tccaaagtca
    23941 tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt cccaatcagc
    24001 caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt tacgatcagt
    24061 gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt aaacgcaccg
    24121 aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata gaatttgagc
    24181 aagagtccca ttaccactaa ccccaaacca taaaggtaag gagtcagttt ccagatccat
    24241 ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata ccagacaact
    24301 tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag tgctacatat
    24361 aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc ataatcaata
    24421 cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc ttaaaaacca
    24481 tactgctgat tattatagcg gtccagttag ataatggaaa gtctttccat agacatttgg
    24541 gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa agcctactgc
    24601 ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc agctgagatt
    24661 ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt cggcaatctc
    24721 tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaatatc gatggaaatt
    24781 caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg agcgaccgat
    24841 tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct tcttatcttg
    24901 gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt ttgcttctct
    24961 tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt ccatcaaaga
    25021 gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat cggattcatg
    25081 tttcgctact tgttctaatg attcatgaga attcgtgatt tcgatctcaa aatgcacttt
    25141 aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa agatagacag caaaggtgtg
    25201 ggaagcaacg atccgacata cttcttcttc attttgttca ttgagcaatt tttgctgagt
    25261 ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg cttgcttagt
    25321 tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt cccgctccaa
    25381 ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg ctgctctgga
    25441 aaaatttctt gtttcatata ctgctcgaaa agtcgttaat aacttaaaca tggcaccact
    25501 tccataactt tttatgataa ttactataaa aactatttga tttaaatata caaaaaacca
    25561 tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt agatgaagac
    25621 aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat gtattcgttc
    25681 atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag aactttttga
    25741 aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt ggaaatctgt
    25801 ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct atgattctta
    25861 tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa acgttgcatg
    25921 atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt atttgtactt
    25981 attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg tgcgctgtta
    26041 tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa gctgatcttt
    26101 caattgaagc aatttatcca agctggttta tcatttacgt tggatttggg gtcatcactg
    26161 taactgccgg gaacttcttc ccacttattg gaaaaatttt cttttggatt tcattgatct
    26221 gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga aatatggctc
    26281 atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc ctaacaggtt
    26341 atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc gctttatctc
    26401 aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt cgtttttatc
    26461 caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata tttactactg
    26521 ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta aaagttgcgg
    26581 aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta cgtttcttaa
    26641 ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa taatcgattc
    26701 aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac aaaaacagct
    26761 gttcatgttt ttgtactggt tgggcttatt accgcagatg caagcttttg aacaccgttt
    26821 accaagaggt cgtaaaagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt
    26881 gcttctcaaa tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    26941 tttattcgaa acaagaggtc gtacaagact tttgtcacga cctcttgttc ttttgtcaaa
    27001 atactggttt agtgctacta tgttcgagta tgcttttagt atttattgac gaaaggaacc
    27061 gatcaaatga aatcattgtc acatccaata aaatctgtaa aggattcagc ggtccgcgaa
    27121 atcttacccg gtttactgct ttcttttgcc atcgctgcct tcagcaggct tcttgctatt
    27181 tatctgccac agttaggtgg agcgactatt gctattttat tgggtatttt attaggaaat
    27241 acttttttta aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag caaactatta
    27301 gaattctctg tttttttact aggtgcaact gtcacagctc aaacgattgc tcaaattggt
    27361 gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg tgcttacttg
    27421 attggtaaaa aactgcaatt cagcgatact tcttctttga tgatggctgg aggaaacgct
    27481 gtttgtggtt cttctgctat tgcttcaatc gccccttcca tccatgcgaa agaggaagaa
    27541 aaaggacaga tcatcacact agtcaatcta ctgggtactg tgatgatgtt gactctccct
    27601 tttcttggat tacagttgtt tggtggaaat ctcattgaaa aaagtgcact tttgggtggt
    27661 acactccaat ctgttggtca agtagtcgct ggggcaagct tacttgacaa ttctgttgtc
    27721 gaattcgcca tgctatttaa gattctacgt attatgatgc tcgttgttgt tgtctatctg
    27781 tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg aaacgccaac tgatacgcag
    27841 aaaaaacgaa aattaccgct tccttggtat gtattagggt ttgtgatctt ttgtttggtc
    27901 aacagcttgt tttctttacc tgtagttatt gaccaatcgg cacattttat cagcagctgg
    27961 tttgagatca ctgctttagc ggcaattggt ctgcgtttag actttgcaaa attcatgaaa
    28021 gaaggtacac gcttcttatc ttatggatta gctgtcgggc ttctccaaac agtgatggct
    28081 ttactattta tttggctgat caaagtttag aatgaacatg aaaaaacagt tgtgattctc
    28141 gcatggaaca atgtccgaat cacaactgtt tttaattttg cagatatcct tctaacaaca
    28201 acaattggcg tttgatttca accccatcta ttgcgtttcc catatatcct gtcagttttc
    28261 cattttgccc gatcactcga tgacaaggaa taacgattgg caatggattg gttcgatttg
    28321 cctgcccaat cgctcgcaca gctttcggac tatcaacagc aacggctact tctttatacg
    28381 ttcggatttc tccataagga atggtcgaca aagctttcca gacattttgc tgaaaaggcg
    28441 tacctctctt tatagataaa ggaacatcaa attcttttct ttttcccgca aaatattctt
    28501 caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc tgattcgctt tctgaaattg
    28561 gctgaaaaga tacagcagtc aatccggatt catctgcatc taaccataaa aagccgatag
    28621 gtgtgtgtat tttcattttg tttgctccta gtcaatagac tggaacttcc tcctttttct
    28681 ctatccaaaa ctacttgcca actaataaaa attgttctta aaagtttgat aaaccgagga
    28741 aacatggtaa actaaatacg ttagtttaca aaaagaatac ctgttctttt acgtaaaatc
    28801 aaattttaaa ggtgttattt aaggtttatt aaattttgaa tgtaaaccat atataaacat
    28861 acgattgaaa ggatagaaac gatgaaagta tcaattgttg tcccatgttt taatgaagaa
    28921 aaaactgttc ctcttttttt tgatgaggtt gaaaaattca aaggtgccca tgagttcgaa
    28981 tatttgttta ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa gaagctagcc
    29041 tcagcttatc cttatgtccg ctttgtctca ttttccagaa attttgggaa agaagcggct
    29101 ctgtacgctg gattgcaagc ttctacagga gatttagtga ccgtgatgga tgttgatctg
    29161 caagatccgc ccgaactgct tcctgagatg atcaaccgga tcactacttc tgatatcgat
    29221 tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag tttctttgcg
    29281 aaaaaattct accagttgat caataaaatc agcgatacag aaatggtcga tggcgctcgt
    29341 gatttccgct tgatgactcg tcaaatggtg gatgccgtcc tagaattaac agaatacaac
    29401 cgattttcaa aaggattatt tagctgggtc ggatttaaaa cagaatatat ttcttatgaa
    29461 aaccgcgaac gtgtggctgg cgaaacatct tggtccttct ggaaattatt caactactcg
    29521 attgatggta tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt tatcggcgct
    29581 ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta ttttgcgggc attgttgttt
    29641 ggtgatccta catcaggatg gccttccatg gtctcgatct tcctgttcat tggtggaatc
    29701 caattgttat gtcttggaat catcgggaaa tatattggga aaatcttctt agaaacgaaa
    29761 cagcgtcctg tttatatcat aagagaaaca gaaaaagtga agccaaatga attgaagcct
    29821 caccaataat atacgtcaaa tgaagtaaac atttttttaa atgaaaaagg actgtgacaa
    29881 aagtcttgtc acagtccttt tttccgaata aatggtgttc cagaagtagc ttcttcggaa
    29941 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    30001 gaagctgacc acttctatca caacctcttt catatcttat tacttcttca ttgctgcttt
    30061 tagtgctgct gctaatgggc tttcttctgg ttcttctgct tgtgcatatt ttttcagcaa
    30121 gcgtcgttct tcatgtttgg tcattttctt cttctgctct tttttatcaa gcaatttttc
    30181 tgtcgttcca tcgtatttac aacggaaata agcaccgttt ttaccttcga tgatttccat
    30241 tttccggtga cattgcgggc atcgatgatt agagactttt ggatcttttc ttctgcggta
    30301 gttgcaatct gaattggtgc agacatagat tttgccgtca cgtgtgtttt tctcgcgcag
    30361 attggcccca cattctgggc atttcttttg ggtaatcgag aaatcttgat attcttgttt
    30421 gcttgatttg atctcattca ccaagcgttt tgtttctttt tcgattccat tcaagaaggt
    30481 tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct ttttcccagc tttctgtcaa
    30541 atctggtgtt actaaagagg gatttaccaa tactaataat tgcttacctt taggagatac
    30601 ttgcagtcct tgaggggtac gttccatcag ttcagatttg attagtttct cgataatctc
    30661 tgcacgcgta gctggggttc ccagactgta tttttccatt tgaccaagca agcttgcttc
    30721 tgacaatggt ttaggcggtg cagatagttc ttttttgatg gcgaaatcgg gattgattct
    30781 cattcctttt tcccatttga ctgtttccgt ctcagtttca gtttcgcctt tccatcctgc
    30841 ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta ccaaattcaa ccgtaacttt
    30901 tgtttggctg actttatggg gttgagcaaa cagtcctaaa aaacgagaga caatcatttg
    30961 atagattttt cgctcttcat tacttaattt ttcataacgt gctctgtttt cagttggcaa
    31021 aagtgcatgg tgatcggtca ctttactgtc ttggaatact tttgtctgtt ggacgatggc
    31081 tccgtttttc aaatactgct tgacctcagg agcaaagtcg ctgactgctt gcaatcgttc
    31141 tttcatcgta cttttcatgt catttgtcag atatttgctg tctgtacgcg gataagtaac
    31201 gatcttatgg gtctcataca aactttgtac aagacttaat gttttctttg cagaatagcc
    31261 atagcgttga ttggcttctc tttggatctc tgttaaatca taaggcaatg gcgcagattc
    31321 cgtttttaat ttttcttgga cttctgtgac agttcctttg cttgtactga tcaattgaac
    31381 aagtttttgt gcttcttctt gtgtttttaa agcaaattga tttttttgaa gcattttggc
    31441 ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt tgtggacgga actgctcgat
    31501 tttcttttct tggtctctca ctaatgctag tgtcggtgtc tgcacacggc ctgctgataa
    31561 attatccgcg tacttcacag ttaaggctct ggtcacattc aatcctacca gccagtccgc
    31621 ttttgcccgg gcaagagcag attcatacaa tgcatcatat tctttcgcag gtcttagttg
    31681 cttgaatcca gttttgattg ctttatctgt ttgagaagag atccataacc gcttcactgg
    31741 tttattgaaa tgcacatact ctaagatcca gcgagcaaca agttcccctt ctcttccggc
    31801 atctgtcgca atgactgctt ctgagacatc ttttcgctga gccagctgct tgattgcttt
    31861 taattgatgt cctgttttag gaagtggttt gattcctagt tttttaggga tcattggcaa
    31921 cgtgtccatt tgccagctct gccattcctt attcagatct tccggcattt tcaatcctaa
    31981 taaatgacca agggcccaag taacgatcac cttgggtcct tcataataat ttttgtgctt
    32041 ttgattagca ccaagcacct tgcttaggtc tttcgcaacg cttggttttt ccgctagaat
    32101 tagttgtttc ataaaattcc tttctgcttt ctactgtgat gggctgatga tttttcaaca
    32161 acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc ttcacaccag cgctcatctc
    32221 cgtcattcca aaaggcgctg agaaagttac tttttgcatt gatatattca taattttgat
    32281 ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc ttcgaaagac tcgaatgttt
    32341 cttcttcttc aatatcttct tcccagtctt caaaaaacca ccaaggctca ttgtccccga
    32401 acatggtaat tacttgatac atactcgtgc tccttctttc agcttttcta gactcatctt
    32461 agtcactttt caagaaagtt gcaagaattt tctcttataa aagggtcatt taatgaacat
    32521 cttaaaattg ttacaaatga atctgattgt ttaaggcaaa gtagcgataa agttattgat
    32581 catccaaaaa tagctgtctt ctggatcgat tgttggttct tttgtaaagt atcctaaaaa
    32641 acgtaaggaa agataaccgt ggatcatact acgaagttcc ctgcttcgat gaactttttc
    32701 ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg atctgattcg tttcatggat
    32761 tccatctaat aaaacagctt cattggtatg cggtacactg atcagtaact catacactgc
    32821 ttgatttgaa aaagcaaagg atcgataagt ttctgcatag atctgcagtg cttcctgacc
    32881 actttttccc acaagagccc gtcgcagctg gtcattaagt tcctgcagaa gtttcgctgt
    32941 tagcgctgtt ttgacatcac gaatattttt gaaatggtta tacagcgagg gagccttgat
    33001 atccaagcgt ttggctaaat gctgaaaggt tatttgctga acatccatct cttctgctag
    33061 ttctcgaaag cagtcgatga ttttctcttg agacagattt ctttttttca tttccatgtt
    33121 tcctctcata aaataaatcc ttcactcttt cagtttacca cgaaaaagaa agagaaaaaa
    33181 gagactttca ttgataaact acatattgta gtttataata aagagctgta gatgaaaatt
    33241 ttcagaaaga aggagtctaa ttggctgcaa gaataaagga aaaattattt aggaaaagag
    33301 aaacttggga acaaaacttg tttgtcttat ggtttggaac atttatggct ggtatcggat
    33361 ttagtttagt catgcctttt atgtcacttt acatagatac gttaggtaat tatagtacgt
    33421 cacaattgaa cttttggagc ggattgactt tttcatctac tttcttggtg actacgctta
    33481 tctctccttg gtggggaaaa ttagccgacc aaaaaggaag aaaattgatg ctgttgcgtg
    33541 cttcattagg aatggcaatc gtcatttctt tgatgggttg cgtgactagt gtgtatcaat
    33601 tagtcgcatt gcgtctgctt caagggattt tttctggtta tatcagtaat gccactgccc
    33661 ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt tcttggaacc ttagcaactg
    33721 gttctgttac aggaacatta cttggaccgc ttcttggcgg gatctctgct tcagcgtttg
    33781 gctatcgccc tacctttttc attacaggga tcatcttatt tttcgtcttc ttgttgagtc
    33841 tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga aaacatggtt tctgccaaac
    33901 agattttcaa agacttgaaa tatccacacg tcgtcatcgg tatgttcgtc acaacgatga
    33961 tcatccaagc atccaacaat tccattagtc caatcatcag tctgtacatt cgccaattgc
    34021 tgcatggaca tgggaatgtt acgttgatca gcggaatcat tgcttcgata cctgggattg
    34081 ccactttgat agccgctcca agatttggtc gcttaggtga caagattggg agtgaaagaa
    34141 tacttgcgat tggtttgggt tttgcgattc tggtttatat cccaatggca ttcgtcacga
    34201 atgtttggca attagctgcc ttacgttttt tgattggtat ctctgatgct tgtctgctcc
    34261 ctgctgtcca agcattgatc acaaaatatt cgcctcatca agctgctgga cggatcttca
    34321 gttataacca atctttccaa gcaactggta acgtagtagg gcctatgatc ggctccagtg
    34381 tttccagtgc atttggttat cgcggcgtct ttctttcgac ttctcttctt gttttggtca
    34441 acttcttgct tgtacgacac aatacacaag agatccatca gcaagaaact gcagaagaaa
    34501 aagcttcagg agtctccata gcttcagggc actctccttc tcatagacaa taagcgtaaa
    34561 tgaaaaacac tgcttgaaca gttgtcacag actgtccttg cagtgttttt gtttaggcct
    34621 gttttttcaa gaactcagat aagaggaata atcccttttc caaggtttct tttttacaac
    34681 agtaacctag tcgggcatgc ttcggtaaat caaaggcact tcctggtact aatagtactc
    34741 ctgtttcttc taataaattg atacaaaatg tctggtcatc ttcttcgatg gctaatttaa
    34801 tgaaagaagt agagacgtaa ttgggagcga caagttcaac tttcggttca ttggctaccc
    34861 attgttttaa aatcacaagg ttctctgtaa tgatcttttt gtttcgcgcc aatatttttt
    34921 ctttattctt caatgcatgt acagcaagat catctgacag gactccgccg caaatcatcg
    34981 tgtagtctcg atactttcga aaaacttctg ccaactcagg tccagtagct gtccagccaa
    35041 tacgaatacc tggaatggag taggttttgg atagggaatt ggttgctata cctttttcat
    35101 agacatctac gatcgacaag aattcacctt tatccgttaa aggcgcatat acttcatcta
    35161 tcaatacata tgcatctact gtacgagcaa tttctgcgat tttttctaaa gttttccgat
    35221 ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca gatgattttt gtttcaggtt
    35281 tgactaaggc ttctagctgt tctaaatcaa actgccaatc ttcttcttcc ttcaaatgaa
    35341 caaagtcaac agcggcacct aaagatttgg ggatgtcata taattgctga taagtaggaa
    35401 gcatcgaaac aacatggtct cctgcttcca ctaacgcgta tagagctaaa agattagcac
    35461 cagtcgctcc attcgtttgt aagatgtttt ccggatcgac ggtttgataa agagaagcga
    35521 cttcttgttt gaaaacatct gatccttcga tccagccata gtccaatggt tcgtttttgc
    35581 gtttttcgaa aaatttactt acgctagtcc catctaagcc aacgagttcg tctaatgtca
    35641 atgcttcgat cgagctttgt gcaatatcat ataccgcttt cttttcccat ttatttagcc
    35701 attcttctac accgaaattt gcgattttca tgtttctttc ccccttatat cttgatccag
    35761 cctacagcaa gcaacccgat tgaaagcagt gcggccacac aaatcagccc aatcattact
    35821 ttttcttttc ccgtaatttg atgtttggtt tcacgacaag cgatccagta gaagcaaaaa
    35881 ccaggaagat aggcaatact acataaaagg acataagaaa atcctgctaa aatgatgccc
    35941 atcaattcaa aaactacagc aatcccaccg atcactactt gttttttgat tccttctttt
    36001 aaagaaaact ggacttgata gatcccgaca agtagataac aaatcaaaat tgccgctgta
    36061 cataaagaat aggcaaattg ataggcataa tcagtaacta agaaagtcaa taagaataga
    36121 ttagtcaaga ttgcagtaac aactaacgaa taagttgggg catttttttt attcgttttt
    36181 ccccaacctt tcggcaacag accgtctcga gccatcaaca tcgttgtttc tgctgggagc
    36241 atcgtccaag acaaccaaga accaatgatc gaaataatca agccgatatt gatcagcgct
    36301 gctccccaat ttcccacgat tccttttaaa atatttgcca tagcaggttg tgaaatagtt
    36361 gctaactctt cttgagataa gacgccgtat ggcaagatcg aagcaaggat atagataaac
    36421 aataaacaaa gcagaccaat gatcgatgct tgctgggctt ctgaacgttt cttcgcgcgg
    36481 ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc acatcataac catcaaacaa
    36541 cctttaatct gcaaccagat cgttccttgc tgccctgttc cattcactag attatcagat
    36601 acgttacccc aaaaatcagc agtgaatata ttcactttaa atgcgataaa tacaaatatt
    36661 aaaaatagga ataatggcac taatttacag atcgtcacga ttgtattgat gacgctcgct
    36721 ccttcaactc cattgtttac taataaagtc agaccccaaa taaaaatact tgcaaataaa
    36781 atagaaggaa cattttgtcc acctttgaat atcgggaaaa attctcccat ggcgctcatc
    36841 agcatcgttg caaaagcaac atttcccagc cacgcagaca accagtaacc ccaaccactg
    36901 atgaagccac ctaatttacc aaatgaagct tccgcgtaac cgaaaatacc accatctaga
    36961 tctggacgct tttctcctaa atgattcagc gataatacca aagctaaaat accaatccca
    37021 acgattatcc atgaaaggat cgctggccct ggagctgcag aactagccag atcacttgtg
    37081 atcccaaaca caccgcttcc gattgacgaa ctgacaacca gagcaaccag tccccatttg
    37141 ctaatacccg tcgatttctt ttccatttcc atcaccctca cttataaaat tgaacaaatc
    37201 acttgcaaaa gattcccttc tattttgcaa ctgctcttgc ttaacaaaaa gcaactttat
    37261 tcatattatt ggatattatg aactttttac acaagtattt caagtataaa atgaataaaa
    37321 attaatttta ttgcaaaaaa acagctatgc tatccaaaag aaaaggtgtt tcttttgtta
    37381 gccatagctg ttttgattca tttaatatcg cttaaacgta ttgtaaaact tttgtttctt
    37441 gatatgcgtg gtcgatcaaa ccaccaccca gacattccat tccatcataa aatacaactg
    37501 cttgtccagg agtgattgca cgtacaggtt cgtcgaatac gacttctgca cgattatcat
    37561 ccaataagcg aactgtcaca ggaacgtctt gctgacgata acggaatttc gctgtacact
    37621 tgaattcttt tggcattggt tcattcgtcg taaagtggat ttcacttgca tccaagcttg
    37681 tggcatacaa tgcaggatga tggaaccctt gccccacata taatgtgttt gtcgctagat
    37741 cttttcctac aacaaaccag ggttcttgcg aatctcctcc gccaccgatc cctagtcctt
    37801 gacgttgacc aatcgtataa tacatcaagc cagcatgttg tcctttgact tcaccatcaa
    37861 gagtgaccat atttcctttt tttgctggca gatagttgct caaaaattgt ttgaagttct
    37921 tttcaccaat aaagcagatt cccgttgaat cttttttctt agcagtcgca aggcctgcgc
    37981 gttcagcaat tgcccgtact tctgattttt ccatgccgcc tagagggaac atcgtttttg
    38041 aaagctgttc ttgtgataat tggctaagga agtaagtttg atctttatta ttgtctactc
    38101 cacgaagcat gtggaccacg ccgttttcat cgcgagtgac ttgcgcataa tgtccagtag
    38161 ctacataatc ggcgccaaga tccattgcat aatccaagaa tgctttgaat ttgatttctt
    38221 tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg gtattcggct aggaaatatt
    38281 caaatacacg atcccaatat tctttttcaa aattcacaga gtaataagga atcccgattt
    38341 gagcagctac tttcgctaca tccttataat cttccgtggc cgtgcataca ccgttttcgt
    38401 ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc gtatccttgt tcctttaaca
    38461 aaagtgctgt tacagatgag tcgacaccgc cgctcatccc tacgacaacg cgtgttttgc
    38521 tgttgtctgt catgatatca ccatcatttc aattagattc tgaaacatct acgagttgaa
    38581 ggtcgcaact tttcagtatt ttacattata cgtcatattt ctgaaaattt catcccttaa
    38641 ttatcaagtc gtaaaaatac tgttctactg gacctattca gtacacaaac atgatagtca
    38701 accaaaaaat cactcatctt ttgagcattc gatataaaaa attggcatac tcataaaaag
    38761 ggccagcctt tcggcaataa ggaataattt tataaattgt gaaaaactat ttttcaaaat
    38821 tgcttcttat cgcttaaagc tgcacgtctc atttacaacc ttttcttttt agatgttatg
    38881 agttgctcag ataagtgatt ttgtttactc taagaaaaaa gggaggcaaa agatacatga
    38941 attactttat cagtgatatg cactttttcc atgaacgctt attaggaaaa aacgactttg
    39001 ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca gttgatcact agctggaatg
    39061 cagtaataaa agaaaccgac catgtttatc atctaggcga ccttgctatg catccgcaat
    39121 atgaaaaagc aaatgcagaa attttggaac tggtcactca actgcaagga acaatccact
    39181 tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta tttagaaaaa aatgatccta
    39241 ggcttactga cggaagtccc aaattttatt tttatgatgt aggtgccatc gttaaattcg
    39301 accatcaaca atattacttg actcattatc cgatgttgct cgggcctaac gacactatcc
    39361 gcaatcttca tggacatatc catcattaca gcgtacctat cgagaacgat gtcaatgtag
    39421 gcgtagacgc accggaaaga gaactcttaa aggaaaaact accatttggc actccgttat
    39481 ctgagcaaca aatcaaagaa atctatgaag caaagaggaa gtacgatagg tcgtgaaaaa
    39541 actgccctat atcaagtatt aaacaaataa agataattac tcatattttt catttgagta
    39601 ggcttattgc cacaggaaca ttaggatcag aaatggagtt gtaacaagac ttctgtcaca
    39661 actccattcc atactattcg tctttttgct catggtgttc ttttaccagt tttttctgta
    39721 attctttatc ataatttcgg gcagctgacg ttaaaaataa aacgattccg cataataaaa
    39781 tcccgattcc ggaaaacaag aacatttttt ctacccctaa acgatcagct aatggtcctg
    39841 caaataataa tccaattgga cctgccaggt tcatcaaaga gttgaagttc cctaagacac
    39901 gacctaaaac attcgattca taactctgct ggatcattgc catcagcagt gtattgaaat
    39961 atggcgttgc acaacctgct cctgcattca agataagaaa gtacaagaac ccttgactat
    40021 cgtttggaac caatccgctt gcaccgatcg tcaggcccat aatgatgtag gctataataa
    40081 taggtttcat tcggtctttc catgttccta taaaactgat cagtgcaccg cctaagagca
    40141 ttccgactgc gtagactact tccactgctc ccgcctgtcc aacggaacca ccaaagtaat
    40201 ctagcgtcat caatggatac agactgattg ctggcatgaa caacagcatc acaaatgcac
    40261 cattgatcgt tatgtaccat aaccctttat tatccatcaa ttgctgcagc ccaaattttg
    40321 catctttaaa aatagtaagc ggaacttcca atatttcagg agaaactttt gggatcttca
    40381 caaaaatcaa taagccgaca ccaaaaactg cccccaatac atctaataaa ataaggtaat
    40441 tgacaggaac gactgaaaac aaagccgcac ctagagctgg cgcaatgatg aaattcgctg
    40501 attggaccat tcctaactgt ccattcgtcc ttgttatgtg agaagatgga acgattgtcg
    40561 gcataatcga ctggatcgtt ggcatttgga atgtttgtgc gaccgaacgc ataaacaaag
    40621 acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt tccgactact gctaaaataa
    40681 gcgcgaaaat cgctacaata atatccgtga caatcaaaag agctttttta tcccagcgat
    40741 ccactagcgg tcctacaaat gggcttagta aaaccatggg aatcattcct agtaaagtag
    40801 caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa ataccagatg atcgcatatt
    40861 ggacgaccat actagtgatt cctgacaaaa actgtcccga taaaaagaac aaataatttc
    40921 tcttccaatt cgttaaaacg accgactgtt cattttccat aaacatccct cttttctttt
    40981 tcattttgta tagcgtttgc acaacaatca ttatacggga ttcttttttc aaaaaatacc
    41041 tttttcccta aataacaatc gtttcaattt aaatttcttt acttagaaac aataaatcag
    41101 caaaatatta aaaataacaa catgtgttaa atttaaaaat aaaaaagaca actaatttgc
    41161 gtcgtcctta caaagttagt catctaacaa taggccgttt caaaactagt tgtcttttta
    41221 tatccacaga tcattcgact ttttcgaata acccacggct aatcatccca tccatcagaa
    41281 aataaaattt atcttggctc attttctgtt ttggatcttt gaaaatattg actgctttca
    41341 atccatttgc tgtagtgatc gacattttca atgtttgaat atcttttgat acagtgattt
    41401 ttaatttgaa tccttctttg ctttgcggcg tggcatctaa tggtcggatc aattgataat
    41461 tgcctccgtt tgtttctgta aatccattat cgcgcaatgt atatcttttt gcatcaggat
    41521 gcaaacgata gctaattggt gcgcccagta ccgttgctgt tgtttcaaat gccatgattt
    41581 tcacctcgat aaaattagtt tctctttatt ataaaagttt atcctgattt ttactaggtt
    41641 tttcagttaa gttccctaaa aaatgaaggc gttctctcag acatctgttc atataagaat
    41701 gacttcacta gttatttttt gacagcacga actaattgat caaggaaata ctggatgtcg
    41761 tcttcggtat ttcccaaacc aaagctgata cggatggatt cttttagtat cggtgtatct
    41821 tctccataca ttgcttctaa tacgtgtgaa ggctcgatat ttcctgctgt acaagctgaa
    41881 ccagtagaaa tagcgacccc attaagatca agcttcatca gtagcaaatc atttgcttcg
    41941 ccaattaagc gaagattcaa cacatgaggg agtttattcg ttaaatcacc atttacttga
    42001 taagggattt cgtttgcttc taatgtatca agaatcaatt gctggaatcc tttatatttg
    42061 ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa ctgcttttgc cataccccag
    42121 atccctgcta aattttctgt acctgcacgc cgcttttctt cctgctcccc gcctaaaagc
    42181 aatgcaggga tatttactcc gtctcgttta tataagaaac cgacgccttt tggcccattg
    42241 attttatgag cagagatact taataaatcg attcctaatt cttctggatg gatcgtttgg
    42301 tttccgtacg cttgtaccgc atctgtatgg aaataagctg gatgatccgc taaaagttca
    42361 ccgatttctt tgattggcat caaatttccg atttcattgt tcccgtacat cacagagacc
    42421 aagatcgtat cttctcgtaa cgcttttttg acctcttcga tcgacagatt ccctttttca
    42481 tctacaggca gatatgtaac gtcaaacccc tttgtctcta aatatttcat ggtattaaga
    42541 acagcaggat gttcgatagc tgtagtgatt agatgtctgc cttctttttg acgcgagaac
    42601 gctgtttcta agatagctgt attatcccct tctgttcccc cactgttaaa gatgatctcg
    42661 tgaggttttg cttgcagact gtctgcaatg atttgtcttg ctgtttccaa tttttcatgc
    42721 gcgtggcgac caaatccatg tatgcttgaa ggatttccaa acgtgtcttg catgattgcc
    42781 gtcatcgctt caattacttg tggatgcatg ggggtagttg ccgcatgatc cagataaata
    42841 gttttcactt cattcatccc ttccaaatta ttgctaggca cattctagca taaaatagcg
    42901 aacaaaccaa agaagagctc atttttgtct agaggacgtt tcatgggcat agaaaaaagg
    42961 agctgtgaca aaagtcttgt cgcagctcct tgaagctgct cacttctgtc acaacttctt
    43021 tccctctttt ttttgtttat tcgaattctt tttcttccag acggaataac ggactcatcg
    43081 gtgtattttc atggatgcgt ttgaccgctt cacccattaa ttctgaacat gtaacgattg
    43141 ttaagttttc tggatgacga ttttcttctg tgaaaacaga atcagtgata caaatatctt
    43201 tgattggtgc attgtctaaa atacttttcg catgatcgga caataatcca tgtgacgcac
    43261 aggcatatac ttcttttgcg ccgttttctt ttaatacatt tgctgctcgt gcaagcgttg
    43321 ctcctgtgtt caagatatca tctaccatga tacatgtctt tccttgaaca tcaccgatca
    43381 cataaccacc ctcagttgaa gcatcatctg catgatctac gatggctaat gtgctgttca
    43441 aatattctga aagactacgt gcacgctgaa caccgctgtt ttttggcgaa acaactacga
    43501 tgtcatctcc cgtcagacct aattgacggt aataatgagc aaacaacggc atcgtaaata
    43561 aattatctac aggaatatca aagaatcctt gtacttgaac cgtgtgcaga tctaaagtca
    43621 gtactcgagt tgcccctgct tcttcgatca agttagcgat caatttcgca gtgatcggtt
    43681 catgaggttt cgccgtgcgg tcttgcctag catacccata gtaaggcaat acgacattga
    43741 tcgttttcgc acttgctcgc ttcattgcat cgatcatgat cagtagttcc atatagtaat
    43801 cattgacagg tgcattcgtt gattgaacga tatacacatg atcccctcga atactttctt
    43861 cgatattgat ggaaatctcg ccatcactaa attgcttcac gacgctttta cctaattctg
    43921 ttccgaacac tttcgcgatt ttttcagcta atggacggtt gccattcaaa ctaaaaatct
    43981 tcaacgtgtc atcctgatac ttattactca tgtagtcctc cccaaaactt ctctattctt
    44041 cgatagttta acatattttt ccaagatttt ctgttaattt cagcaaattc ttaaggaagt
    44101 gaaactgttc cgttactcca actggcagga atgactttat gataatcgcc ccagttgtcg
    44161 tgtactttgt aataacgttc tcctgtttca gtttcaaaat aagcgtacgc tgtcacccaa
    44221 tgattgccat atgtactgcc aagtactttc aaaataccaa tcatcacagg tttgccttca
    44281 cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag ttgctcgtgc acgctcatga
    44341 ttgccattct tctttaaaaa actagaaatc cctgtactaa cctgaaaagg aatcgtagga
    44401 agtcctagtg gttggatctg gctacgcaat gccttcgtca aaaccaacga atcagccgta
    44461 tttttcttgc gaatctcatt tggaagcatc caccccttac gatagtcttg ataataggca
    44521 aggagaactg ctgccgcgta agtaccacaa agatagcctg agggggtagc ccatttctta
    44581 tacctcaaaa aacgttcttc tggcaatccc acccagcaat ttgcgagttc ctttcctgac
    44641 cttttttctt cgatcaaaac gggaatatct tcttttctgt tgatccatct agataactcg
    44701 attggctctt cattgatcag cttccatttt ttacaagaaa gaccctcttg ataagtatat
    44761 tttgctggag aatattctac cggatgtcct gattgatcga taatcacata actttgatct
    44821 tcaaaaacca gagctaaaaa aagcaattta ctttttcttg actgcagctg ctgagtgctg
    44881 gctaattttt taattttttt atctactttg aaatataaag gtttatccac tacttgattc
    44941 agttgggatc gcctcctttt tttcattata agtgaaggat tgaaaaaaac aaatcagttt
    45001 gattgttcga attttcaatt ttttatgttc tatttgttaa ttccttcatt ttgtaagata
    45061 atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa caaagaacga ttgaaacaag
    45121 atttctatga agcagtcaat gaagaatggc aaaaaacagc aaaaatccct gcagataaac
    45181 cagctacagg cggtttccaa gatttagttg acgggatcga ccaacttttg atgggagaaa
    45241 cagacgatct gcttgcccat ccagaaaaag tgtcttcaga aaaaatgaaa catttccttg
    45301 catattatca actggcaaac gattatgacc gaagaaatga gttgcatgct caaccgcttc
    45361 aacctataat cgaaaaagtt gaatcattgc agtctttcga cgaattgaat aagcaatttc
    45421 cagaatggct gttggataca ttaccattgc catttaatct ggatgtagat gcagatatga
    45481 agaatgcgca aacgaacgcc ttctttgctt atccgccttc tttattcctt ccagataaga
    45541 cgtattatgc atcagaccat cccaacggtg cacaactaat aggcgttttc tttgatatgg
    45601 ctgttcagtt attagagctt gccggcaaag aaaaaagtca ggcagaagca atcacggaac
    45661 aagcagtgca atttgataaa ttgattgctc ctcatgtaaa aagtgcagaa gaaaacgcag
    45721 attacagcaa aatgtataat ccgcaatctt ttgatacatt tattaaatac acagataaac
    45781 tggacttgaa aaaacttgtc acgggtttga ttgggacaac tccagataaa gtgatcgtaa
    45841 ctgatccagt gtacttcgat catttgaatg acatcctgac agaagctcat tttccgttat
    45901 tcaaaagctg gatgatcatt aaaacagtac gttctcttag tggttatcta tcagaagaat
    45961 tccgccaagt cagcggaatc tattctcgcg tattgtctgg aacagacgaa gcaatgcctc
    46021 agaaaaaagc agcgtattac cttgcctctg gacaattcga tcaagtagtt ggcgattact
    46081 acggaaaaaa atattttggc gaagctgcta aaaaagacgt agaacaaatg gtgaaaaaaa
    46141 tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg gcttggggat gcaacaaaag
    46201 aaaaagccgt cataaaatta aataacttag gtatccaagt aggctacccc gataagatcc
    46261 cagcattatt cgaccagttc aaaacagtac ctgcagatca gggtggtact ttgctttcaa
    46321 atactcttgt tttcagcaaa ctcacattaa aagatcgttt cagcaaatgg aacaaaccag
    46381 tggatcgttc agaatgggaa atgagtgctg acacggtcaa tgcttattat catccatttc
    46441 ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc attttacagt ctagaacaat
    46501 caagctctgc taattatggt ggaatcggag ctgttatcgc tcatgagatt tcccatgcat
    46561 ttgataataa cggcgcttta tttgacgaat atggcaactt gaacaactgg tggacagaag
    46621 aagatcttac acaatttcaa aacaaagcac aagcaatgat cgaagaattc gatggtctgc
    46681 cttttgctga cggaaaagta aacggaaaat taaccgtttc ggaaaatatt gctgatgcag
    46741 gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc agatctttct atcgaggaat
    46801 tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa aaaagaatac caacaacttt
    46861 tactgcaaat cgatgtccat gcccctgcta aactccgagc aaacgtgcaa ccaaaaaacc
    46921 ttgacgaatt ctatgaaaca tttggtatca cggaacaaga cgctatgtat ctagcgccag
    46981 aaaaaagagt gcgaatctgg taagaggttg tgccagaagt ggtcagcttc aagaaataag
    47041 aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat ttcggcttat ttttcgaaga
    47101 agttacttct ggaataccat gtattagagg ttgtgaaaaa gctgcttagt ttcaagtagt
    47161 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact tactaccgag
    47221 aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa tcagcgtcct gacctgagta
    47281 ttagagaaaa aaacggaaaa atagttcctc atattttgcc gtttgttgag ctaataccga
    47341 gggtcagctg attgaacacc gtttagtgga gagattgtga aaaagctgtc ctgcatcaag
    47401 taataagaac agccaatcaa aagcagcaag gagaacctca tctataaaaa gatagaagga
    47461 agtgagacat ctattggtct catttccttc tatttttact ttcctcagtc aattgtttta
    47521 catcataaaa ggagaaattc cacttatttt tttcgctgtt tcatgacata aacaactaca
    47581 ccgattaaca aaattcctcc agctaatgct agcaacgggt tattaaattc tcccgttttt
    47641 gggagtcctt gagtgccatt tttttcactt tcttttgctg tcctatccgg atgagaaggt
    47701 tttccatttg gttttccatt tggtgattca tttcctggat tttctctttc tggtttgtca
    47761 tttcccgggg gcacatcggc agtttctcgc acagtgatcg ctttttgctg ggtaacagat
    47821 tctgtatgag cagaccattt ttccccatca aatatttccg aacgataagt gacttctagc
    47881 gtatattctc cttttgaagg tgctgaaaaa atagcggtat gattagaaaa ttgagcactt
    47941 gactgtgttt caacgatttg gatcttttct gggaaataac gcatatcttc aaagacaggc
    48001 gttccttgat ttggtgtgaa agttgacaca aaaactgtaa aaggcttcag ttcaatgata
    48061 tttccttctg cattctccgc cacaccattt gcttcttgta taataatcgg attattttgt
    48121 tcatttggtt ttgtctgatt tggttcttgt acgattgcat actctggata ttccgaagac
    48181 ggtgaggttt ctccgtcaaa aatagcacct acagataatg gaagaccatc cactctttcc
    48241 ttttctgttt gacgattccc cggtttaatc tgcatcagtc catgaacatg gatacttcca
    48301 ttttcatagc gcgtaacagg ctgctgacct tctttcgaac atgtatttag agactcgatc
    48361 caatcttttg atatggtatg tccaagagta ttttctgatt gtttctctcg ttcattccaa
    48421 tagtagttca acgagtggta gatttgatct tcgttttgat tcaatgggcg gatctgctct
    48481 ctcatctcaa gattctctgt tcggaaagat aagataccag aagcatggta gtctgtgttt
    48541 cctgctgaag aggtataaag agccacatta tatgaaccgt tattaaaact tgtactgcta
    48601 ttgacgataa tatctgttcc gctattggaa tcgattcctt tcgcaagatt ttcgaaagac
    48661 aaactattga tcaattcatg agggcccgtt aaagaagaac cgcccatttt gaatccatta
    48721 ccgttacctt ctccctcaat tcctggtacc cagccgtttc gataagcaac tgagtttttg
    48781 atgattactt ttccgatcga acctgtttca tttttagcaa aaaggtccca accgtcatct
    48841 gcattatgat acgcgataca accgtcaaat acatttcctt ctcctattgt cagtttggcc
    48901 gcaaatccat cggcatcttc aaatcctgga tcggcatttt caaaagatgt acagttcttg
    48961 actaagttat gcgcgggcca tgcttcgaaa ggatcatttg agctaccgct gatttggatt
    49021 cctgtatttc cattccgata agcatgaatc tcttcgatca aattatgatt tccagaaatc
    49081 tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa accctcgtaa accccagtag
    49141 tgactgaaca gtgtcatccc atttccagtt cctttaaaat caagtacagg acgcttgttt
    49201 tctggatttt tctctgcaat caactgaatc gggttttctc ttgtcccgtt aacgcctttt
    49261 ggaatcgtca atcctctttc gaaggaataa gttccaggcg ccatgacgat tgtctgccca
    49321 gcatatgcgt atctcaatgc ttgtgtcaga gataatggat tttgtctgct tccgtttcct
    49381 gcatctgacc cttctggtgt tacataaact gtcgaatatt taggtttacg gtagtccact
    49441 gtatgaagaa tctcttttgt ttcataacta gtcatctcta cgtattcttc tggcggatag
    49501 tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc cttccatcag ttttgtctga
    49561 taatcaaatt cccgattcgc agtgatagcc aagttttttg cgttcttaag cacctctcct
    49621 gttgctttat ttttaattgt caataatccg tcagcatttg ctctaaagga catagtatga
    49681 tcttggtttc ctgaatgatt actagaggtc acagtgtaaa tcggatcaat caagcgagta
    49741 ggtctttctt gcccaggagg atctgtcgca ggatctgagt aagcaacttc gattccttct
    49801 accttgatcg tcatattcct tgctgcagca aatcctgcat aaataaccga ttcatctgaa
    49861 gcgtataatt tttcccagtc atacatgatg tcagaagcca tttcttctcc tgtttcagga
    49921 tcgtaataat atgcctctaa accagtattc gtttttttca tctctacgtc cagtttagta
    49981 atcgagttcg tggccggaaa cagattccca tgaccgtttc cgaatatatt ataagaacct
    50041 gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg ttgtttccac tgagacactc
    50101 ccaggagctg gcgcagcttg cggatcttca gatgatattc ctgtgattgc ccgtgtcccc
    50161 aatccaagtc gcatcgtata tttgctgccg ctcgtgttga ccacttcttg tctatccgag
    50221 tcccagacat attcgatccg actgcctagt atggcaaatg aatttgaata aaatgcctgc
    50281 ccaaacaaat gattttttgg tactgcgtct cttaccatta aagcaaatcc ttcttgggca
    50341 ttcgtatagg tccaagattc tatttcgatc gtcgcccgca tcctaaagtt gatgtctttg
    50401 gggattgctt gataataata ggtcattcca tctgatccgc ttgactggaa ttttccgccg
    50461 ttattcgtgg aagagagccg cacgccgtct tctaatggtt caatggcata cctactagca
    50521 cttgtgcttt ctccgaaaac aacccctttc cactcttcat ttgcttcttc agattgcttt
    50581 tttgtctttt cattttctga ttctttcatc ataggatcat ttccttcttc cgtatccata
    50641 tcttcactag ttcttggtat ttcctccatt gttgacatcc cttcaatgtt cacgtgtgca
    50701 ttttcttcgt tcacagactg ttgttcgtta gcagcaactt caacgacaga aattaaaacc
    50761 ggggtactga gcataaggag attcatcatt agcgcactgt attttatacg tttttttaac
    50821 aagtgtattc ctcccgtaac aaattagaaa gcgtttactt aaagtattac ctaatttatt
    50881 ttta
//
LOCUS       DAJSVF010000013        48223 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSVF010000013 DAJSVF010000000
VERSION     DAJSVF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.013"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..201)
                     /locus_tag="ON550_000722"
     CDS             complement(<1..201)
                     /locus_tag="ON550_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3953829.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFT"
     gene            complement(223..1197)
                     /locus_tag="ON550_000723"
     CDS             complement(223..1197)
                     /locus_tag="ON550_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3953830.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1497..2369
                     /locus_tag="ON550_000724"
     CDS             1497..2369
                     /locus_tag="ON550_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3953831.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIIIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(2951..5149)
                     /locus_tag="ON550_000725"
     CDS             complement(2951..5149)
                     /locus_tag="ON550_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3953832.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            5381..6373
                     /locus_tag="ON550_000726"
     CDS             5381..6373
                     /locus_tag="ON550_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953833.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            6433..7854
                     /locus_tag="ON550_000727"
     CDS             6433..7854
                     /locus_tag="ON550_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR3953834.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPDKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            7847..9346
                     /locus_tag="ON550_000728"
     CDS             7847..9346
                     /locus_tag="ON550_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR3953835.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            9385..10779
                     /gene="uxaC"
                     /locus_tag="ON550_000729"
     CDS             9385..10779
                     /gene="uxaC"
                     /locus_tag="ON550_000729"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3953836.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            10938..11621
                     /locus_tag="ON550_000730"
     CDS             10938..11621
                     /locus_tag="ON550_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR3953837.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            11623..12459
                     /locus_tag="ON550_000731"
     CDS             11623..12459
                     /locus_tag="ON550_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3953838.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            12470..14224
                     /locus_tag="ON550_000732"
     CDS             12470..14224
                     /locus_tag="ON550_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953839.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(14500..14841)
                     /locus_tag="ON550_000733"
     CDS             complement(14500..14841)
                     /locus_tag="ON550_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3953840.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(14909..17080)
                     /locus_tag="ON550_000734"
     CDS             complement(14909..17080)
                     /locus_tag="ON550_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3953841.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            17249..18148
                     /locus_tag="ON550_000735"
     CDS             17249..18148
                     /locus_tag="ON550_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3953842.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(18219..19076)
                     /locus_tag="ON550_000736"
     CDS             complement(18219..19076)
                     /locus_tag="ON550_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3953843.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(19061..21763)
                     /locus_tag="ON550_000737"
     CDS             complement(19061..21763)
                     /locus_tag="ON550_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3953844.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(21776..23065)
                     /locus_tag="ON550_000738"
     CDS             complement(21776..23065)
                     /locus_tag="ON550_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR3953845.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            23215..24261
                     /locus_tag="ON550_000739"
     CDS             23215..24261
                     /locus_tag="ON550_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3953846.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24263..26416
                     /locus_tag="ON550_000740"
     CDS             24263..26416
                     /locus_tag="ON550_000740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR3953847.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(26889..28340)
                     /locus_tag="ON550_000741"
     CDS             complement(26889..28340)
                     /locus_tag="ON550_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3953848.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(28337..30061)
                     /locus_tag="ON550_000742"
     CDS             complement(28337..30061)
                     /locus_tag="ON550_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3953849.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(30054..30668)
                     /locus_tag="ON550_000743"
     CDS             complement(30054..30668)
                     /locus_tag="ON550_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3953850.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(31013..32470)
                     /locus_tag="ON550_000744"
     CDS             complement(31013..32470)
                     /locus_tag="ON550_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3953851.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(32511..33431)
                     /locus_tag="ON550_000745"
     CDS             complement(32511..33431)
                     /locus_tag="ON550_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3953852.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(33443..34390)
                     /locus_tag="ON550_000746"
     CDS             complement(33443..34390)
                     /locus_tag="ON550_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3953853.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(34869..35588)
                     /locus_tag="ON550_000747"
     CDS             complement(34869..35588)
                     /locus_tag="ON550_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3953854.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(35637..37283)
                     /locus_tag="ON550_000748"
     CDS             complement(35637..37283)
                     /locus_tag="ON550_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3953855.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            37462..38052
                     /locus_tag="ON550_000749"
     CDS             37462..38052
                     /locus_tag="ON550_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3953856.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            38144..39649
                     /locus_tag="ON550_000750"
     CDS             38144..39649
                     /locus_tag="ON550_000750"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR3953857.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(39733..41085)
                     /gene="celB"
                     /locus_tag="ON550_000751"
     CDS             complement(39733..41085)
                     /gene="celB"
                     /locus_tag="ON550_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3953858.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(41085..41603)
                     /locus_tag="ON550_000752"
     CDS             complement(41085..41603)
                     /locus_tag="ON550_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953859.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(41619..41945)
                     /locus_tag="ON550_000753"
     CDS             complement(41619..41945)
                     /locus_tag="ON550_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR3953860.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(41958..43937)
                     /locus_tag="ON550_000754"
     CDS             complement(41958..43937)
                     /locus_tag="ON550_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3953861.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(43958..44272)
                     /locus_tag="ON550_000755"
     CDS             complement(43958..44272)
                     /locus_tag="ON550_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR3953862.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            44440..44733
                     /locus_tag="ON550_000756"
     CDS             44440..44733
                     /locus_tag="ON550_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR3953863.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(44811..45923)
                     /locus_tag="ON550_000757"
     CDS             complement(44811..45923)
                     /locus_tag="ON550_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3953864.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            46076..46300
                     /locus_tag="ON550_000758"
     CDS             46076..46300
                     /locus_tag="ON550_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953865.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            46310..46489
                     /locus_tag="ON550_000759"
     CDS             46310..46489
                     /locus_tag="ON550_000759"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953866.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            46683..46853
                     /locus_tag="ON550_000760"
     CDS             46683..46853
                     /locus_tag="ON550_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3953867.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(47277..>48223)
                     /locus_tag="ON550_000761"
     CDS             complement(47277..>48223)
                     /locus_tag="ON550_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HCR3953868.1"
                     /translation="QGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQA
                     PTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQD
                     ANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVV
                     ELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGL
                     APGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSS
                     GNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        14450 a         9748 c         8571 g        15454 t
ORIGIN      
        1 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
       61 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
      121 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
      181 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
      241 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
      301 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
      361 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
      421 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
      481 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
      541 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaagataga tgatcgtaga
      601 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
      661 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
      721 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
      781 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
      841 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaaaatcaa
      901 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
      961 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
     1021 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
     1081 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
     1141 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
     1201 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
     1261 ttacgtatat aatcattttt atcgtatgta aaaacgctta catagaaatg atttttgtgc
     1321 tatgcaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
     1381 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
     1441 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
     1501 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
     1561 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
     1621 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
     1681 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
     1741 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
     1801 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
     1861 gcggggggat gattccaacg ttcttagttg ttcgttctct aggactggtc aattcttact
     1921 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
     1981 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
     2041 gtgtattttt caaaattatg ctgccattag cgttaccgtc tattgctact atctcgcttt
     2101 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
     2161 ctaaatggcc gatacagatt cttttacgcc aaatcatcat tgtatcaagt gggatgcaag
     2221 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
     2281 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
     2341 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
     2401 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
     2461 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
     2521 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
     2581 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
     2641 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
     2701 gacttttgtc acagctcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
     2761 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
     2821 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
     2881 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
     2941 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
     3001 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
     3061 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
     3121 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
     3181 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
     3241 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
     3301 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
     3361 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
     3421 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
     3481 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
     3541 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
     3601 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
     3661 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
     3721 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
     3781 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
     3841 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
     3901 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
     3961 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
     4021 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
     4081 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
     4141 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
     4201 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
     4261 gggcaaatct gtccgaaaat aagtaggaaa gaagaccaat gagtcctacc agtgttaaag
     4321 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
     4381 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
     4441 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
     4501 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
     4561 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
     4621 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
     4681 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
     4741 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
     4801 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
     4861 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
     4921 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
     4981 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
     5041 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
     5101 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
     5161 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
     5221 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
     5281 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
     5341 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
     5401 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
     5461 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
     5521 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
     5581 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
     5641 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
     5701 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
     5761 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
     5821 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
     5881 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
     5941 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
     6001 caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
     6061 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
     6121 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
     6181 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
     6241 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
     6301 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
     6361 caattacttg taaactacaa gtaattttat ataaaaatgt taacgcgtgc ataacacaga
     6421 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
     6481 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
     6541 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
     6601 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
     6661 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
     6721 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
     6781 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
     6841 cccccacaga gcttcccgga caaactgaca gcctttttat accgacgttt caaattaaac
     6901 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
     6961 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
     7021 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
     7081 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
     7141 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
     7201 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
     7261 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
     7321 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
     7381 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
     7441 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
     7501 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
     7561 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
     7621 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
     7681 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
     7741 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
     7801 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
     7861 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
     7921 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
     7981 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
     8041 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
     8101 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
     8161 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
     8221 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
     8281 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
     8341 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
     8401 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
     8461 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
     8521 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
     8581 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
     8641 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
     8701 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
     8761 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
     8821 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
     8881 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
     8941 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
     9001 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
     9061 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
     9121 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
     9181 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
     9241 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
     9301 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
     9361 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
     9421 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
     9481 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
     9541 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
     9601 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
     9661 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
     9721 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
     9781 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
     9841 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
     9901 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
     9961 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    10021 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    10081 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    10141 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatcgt
    10201 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    10261 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    10321 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaatcgt
    10381 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    10441 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    10501 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    10561 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    10621 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    10681 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    10741 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    10801 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    10861 cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt
    10921 atgaaagaag agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa
    10981 aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac
    11041 ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg
    11101 aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat
    11161 ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt
    11221 taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac
    11281 tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg
    11341 gagactatcc aaaagcaatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg
    11401 tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct
    11461 acctgcatct tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc
    11521 actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat
    11581 cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta
    11641 ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca
    11701 cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa
    11761 ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat
    11821 atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt
    11881 gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag
    11941 atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa
    12001 gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt
    12061 ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct
    12121 aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat
    12181 ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat
    12241 ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc
    12301 cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa
    12361 tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa
    12421 gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg
    12481 aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta
    12541 tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc
    12601 atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc
    12661 ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa
    12721 tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc
    12781 tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga
    12841 attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt
    12901 gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg
    12961 tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc
    13021 cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca
    13081 agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg
    13141 agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta
    13201 tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag
    13261 ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg
    13321 catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt
    13381 ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat
    13441 tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca
    13501 tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc
    13561 gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc
    13621 atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc
    13681 aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa
    13741 ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat
    13801 cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca
    13861 actttcttca ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg
    13921 caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca
    13981 gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa
    14041 aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg
    14101 gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaattgttga
    14161 aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga
    14221 atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg
    14281 gtgttctcct gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt
    14341 tttttgaaat tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa
    14401 tggtgttcaa gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga
    14461 acgtctcttt cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg
    14521 aactggtgtc atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt
    14581 ttgcatcaag tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt
    14641 cattgcatct tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg
    14701 catcgcttgg aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta
    14761 cgcttctttc aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc
    14821 gctatcgtag atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt
    14881 aacatggggt tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt
    14941 tagccgcttc accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc
    15001 cttcttttgc ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat
    15061 tagaatttcc tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg
    15121 catctgctac actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt
    15181 tgaagacttt cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt
    15241 cttcgtaacc catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt
    15301 catttgcttt tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat
    15361 aacctgcagg attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg
    15421 tcatttcatc ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga
    15481 cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac
    15541 catcattggc aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca
    15601 gtccaccaag ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt
    15661 ctgctttatc atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat
    15721 tcaaactttg tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat
    15781 tatgcgcatc gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag
    15841 cttccagtgc tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca
    15901 tctgtgtagc aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt
    15961 gtacaccacc tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg
    16021 aagcatttgc tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat
    16081 cctgatctaa agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac
    16141 catactcgtt aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag
    16201 aatctgaatt atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt
    16261 gttcttgttc ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc
    16321 gatcattcgc attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc
    16381 ctgccaaagt agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc
    16441 tggaagcgtc ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg
    16501 tcaagatttc ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg
    16561 cttttcgatc aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga
    16621 tcgtacttcc tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg
    16681 caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta
    16741 ctgcatcttg gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt
    16801 acagtgtgcc agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg
    16861 attttaacgt ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta
    16921 agacacagat caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc
    16981 gctttcgcca tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca
    17041 aagcagcttt tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat
    17101 ttgattcaat cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta
    17161 gttcaatatg tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg
    17221 taagtttttt cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag
    17281 gagtacactt gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg
    17341 cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg
    17401 taaaaacgtt tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga
    17461 ccagatcaca ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga
    17521 tgcgaagcac atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc
    17581 aggaatgaag actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc
    17641 tgcttatctc gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac
    17701 aagcggtgcc gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt
    17761 ggaacaaaaa atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga
    17821 tatgaccatc accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga
    17881 tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca
    17941 aacacaagtc tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc
    18001 tcataaggga catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact
    18061 gatgctccat gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg
    18121 taaagcttta cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat
    18181 aatgataaga ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct
    18241 gctaacagca aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat
    18301 tcacttactt gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt
    18361 ttttgaagga gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct
    18421 aacgtcaacg tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca
    18481 ggatgatctt gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct
    18541 gctgctaatc cttctaaaca atctttatga taaggacaag taccttcata atggtcatct
    18601 ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct
    18661 tcattgatca cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt
    18721 tttccttgtg ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc
    18781 caagcagcag gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac
    18841 ccttttttgg gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc
    18901 gggccgaatg agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc
    18961 tgatccaatg tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca
    19021 ccatctgaaa ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt
    19081 tttcctcctc gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt
    19141 gcttcgttgg ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt
    19201 ccaatttttc taaatgacta gacatattga aacctcttac tactacttta tcactaaact
    19261 tgctgcgttt caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt
    19321 ttgaaatcaa tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg
    19381 cgtatgcatg caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat
    19441 tgaaccgatg ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta
    19501 gttctcccac actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt
    19561 cattcaaacc aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg
    19621 cttgctgatg ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct
    19681 tcaccacttc aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca
    19741 agacacgtaa gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt
    19801 tactatcttt tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc
    19861 gttctgcttt tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt
    19921 ctgcagaaac agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat
    19981 ctactaatac ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga
    20041 aaggttgttt aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat
    20101 tattcaactt tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg
    20161 tcaaatgtag agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt
    20221 cttctgcttt cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt
    20281 tttctaatgg taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt
    20341 tcggcaaatc ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc
    20401 cactttttgt ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt
    20461 gttcaggtat tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac
    20521 cagttttatc cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc
    20581 cggtcgtatc gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa
    20641 cttcatctgc tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac
    20701 cgcaaatact gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat
    20761 catggggata gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt
    20821 ctagctgtcg ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat
    20881 tggctaaagt ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta
    20941 agtcttctgg cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa
    21001 actcgtaatc tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta
    21061 ccggttgatg atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg
    21121 catccgctaa tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat
    21181 tcccgttgct ataccaattg gcaaatagca gaccaaagat tgctgtttga tcaggtcctt
    21241 tccaccacat ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa
    21301 agccaattgg ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta
    21361 attgaggcgt ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg
    21421 ttccccattt acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc
    21481 tgataagaaa atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga
    21541 ttgcttgttg gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa
    21601 tcgtttgccc atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta
    21661 gatcgtctat caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac
    21721 gatcccagtg gctatgagaa atgatatata ctttcttttt catttacgat acctcctact
    21781 tttctactct aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag
    21841 aaaaccattc tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt
    21901 gtgttcctgc gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg
    21961 acgttgtcat tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg
    22021 aggatccaat tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca
    22081 atgttcttct tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg
    22141 cggcgatcag attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta
    22201 cttcatatgc ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc
    22261 cctcttggat ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta
    22321 tcgtttgaaa aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta
    22381 ccagatatcc ataacggcaa gtatcatcgc taggacggaa accagaccat gtcattcctg
    22441 taaaagctgt ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt
    22501 ctcgtacaaa tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga
    22561 tttttttgat accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt
    22621 aaagaagata ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc
    22681 agatccaatc gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt
    22741 tgaaggcatt cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc
    22801 tgatcatgtc agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg
    22861 cagtagagtc tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt
    22921 cattttgctt tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg
    22981 cccagtccgc atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg
    23041 attttggtat atctttataa gccactaata aaaccttctt tctatttgat atatctttaa
    23101 agattatact tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact
    23161 ttttcatcca ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgaat
    23221 caaccattgt accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta
    23281 cctgtagaca gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga
    23341 atcacttcca aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc
    23401 gggaaaggca gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa
    23461 acacgacgga ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc
    23521 aagggattga accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac
    23581 tttctacaaa ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat
    23641 gctcttgata cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct
    23701 gtcgtcatat tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat
    23761 ttccaaggcg gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct
    23821 tatttgtttg gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt
    23881 gaagcattgg acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca
    23941 ttagatgaat tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac
    24001 gatgttgtcg ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa
    24061 gatttttcaa ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg
    24121 acaaccatcg cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact
    24181 tggttagaga caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga
    24241 caatcaacaa aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc
    24301 gaatcagcca aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat
    24361 gaattatttt gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga
    24421 ccgcgttttt caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag
    24481 tcatatgctg tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca
    24541 aagttcttac cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt
    24601 acgttatggc atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga
    24661 ttacagcaaa aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt
    24721 tatagatcct tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga
    24781 tttttctttt tattttatat tgagatttga atatccttta acaattgatg agttagctgt
    24841 ttccaacaaa aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat
    24901 ccgatttggg acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca
    24961 aaaccaaagc aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga
    25021 tcgtatacag ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata
    25081 tcgggtgttt ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca
    25141 ctatgatacg actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg
    25201 ggatacgtat aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga
    25261 aatgctggaa ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc
    25321 tcctgatgaa cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc
    25381 tgtaaaagat atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc
    25441 tacggttcaa agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata
    25501 tggctatgtt cctaatcact accacgaatc agtcaatcac acattagact atgcttatag
    25561 cgatttttgt attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata
    25621 tcagcagcaa tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc
    25681 aaaagataaa gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta
    25741 tgcagaagga agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat
    25801 cggaaaatat ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc
    25861 cccaacgttt gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc
    25921 gatagaattt ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt
    25981 cagttatata ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac
    26041 cttcgacgat tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg
    26101 gtatatcttc aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat
    26161 cggtatgcca ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat
    26221 tgaagcaacg cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa
    26281 agaatatacg aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta
    26341 ccaattaggg atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt
    26401 atcaaactat aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    26461 gccaagcaaa aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa
    26521 gcttttgaac accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag
    26581 agacaaatgg caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca
    26641 gctgattgaa caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa
    26701 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
    26761 actggctttt gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat
    26821 aagaacagcc caaaattaga tttctagaca attttttata agttatttca ttacattggc
    26881 tttttctttt atgattctcc actgttttct cggtactctt taggggatct gccgacgatt
    26941 ttcttgaata atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca
    27001 ttgatgtttt gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc
    27061 aaataagctg aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca
    27121 tttaaatgca ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc
    27181 aggattacga ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg
    27241 atgaatgaca gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg
    27301 attccttgta aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac
    27361 agtaaaaaac tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca
    27421 aaaaaatcag tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg
    27481 aaacttgaaa aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc
    27541 tcgtctttat ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt
    27601 tcagggattt tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca
    27661 ggaaagtttt cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac
    27721 gtgatcaata cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc
    27781 aaccatctat tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc
    27841 tgcttctcac ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc
    27901 aaccattgac tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc
    27961 tgatctaacc gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt
    28021 ttcatcaaat aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg
    28081 tatcctgata aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat
    28141 tccagtccat ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt
    28201 tcaagtacag ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa
    28261 ctttgccaat caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat
    28321 aacacttgat acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct
    28381 cattattctc aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca
    28441 cgttttgcag accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa
    28501 tctgttttag ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc
    28561 gtttgccttc ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac
    28621 cgtgtttaaa ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt
    28681 ccaaatcagg atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca
    28741 aatagacata tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat
    28801 tcgtattgtt tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc
    28861 cttcgctgat tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga
    28921 tctgtgcttg aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt
    28981 cttctacaaa ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccttca
    29041 gttcatattc cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat
    29101 ccatggcctg catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa
    29161 agataagcag tatattgtct aaaataatga taccaatgat cagcggggca agattttttc
    29221 ccgtttcgat ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca
    29281 gttcttggtg ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt
    29341 tcttcagact ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat
    29401 tatcatggaa tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat
    29461 tgaatgacgt tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa
    29521 tacttcctga ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta
    29581 attttggtgt cccctttttt atttccccac ctttgttttc tctagaagat tcataatact
    29641 ccggtagttg attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat
    29701 ctttgatttg atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat
    29761 acatcagata ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg
    29821 aattccctac aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc
    29881 tgcttgtgct ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc
    29941 aagtatggat actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt
    30001 atttccgcaa cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca
    30061 taactttcca actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa
    30121 accgtcaaaa gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct
    30181 aaaattatac cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa
    30241 ataaaactta gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata
    30301 ctgtattgat aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct
    30361 aaaaaaatga gccctttgat ttgaacagat aaatataaat tgtaactcaa taaagcagta
    30421 ccagataaaa ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc
    30481 cagcctcttt ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg
    30541 actgttttcc atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag
    30601 aaatacaagg ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc
    30661 cctaacatat cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt
    30721 tgggcaatca gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata
    30781 ttttgtcgtt ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt
    30841 ttgtgaaata gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac
    30901 atttttacat atatttagct taataccgag aaactggctt ttgaacaccg tttattcgga
    30961 acaagaggtt gtgacacgtc ttttgtcaca acccctttgt ctttattcat tattattctt
    31021 gtcggaactc atcatattgt ttttgcattt ctttcaatac cttatcatag ccagccttgt
    31081 ccaaagcgtc tttcaatttc ggtaatgttt catctggatc aacagtccct gtattcaatc
    31141 catctaaata ttgattcatg acattagaaa tattacttaa ttctgttttg acactgtcag
    31201 tgttgaagct gaatcctaaa atcggtgaag tttctgcatc agcaatcgat tgatcacgtt
    31261 tggcaatcat gtcatccgta atagactctt gtgtatatag aatcttgttg tttcctgtat
    31321 tccaagcaga catatgtgta tttggctgat aaccatctag caatttgatt ttatcatctg
    31381 aaccatcgac tttttcccat gcttctccct cgatccccca aacaagccca ttcaataatt
    31441 ctggatcact gttaagcagc cctagaagtt ctactgattt ttctttattt tttgaagtat
    31501 ttgatacgac aaaatttgcc atttgtgctt gagctgtaga ttttagtgga acagtaattg
    31561 cttttgagac aagttcttgt tttgctgcat ttgtcaaaat tgtatcgcca taatcatacg
    31621 gtccttgtgt ttctccacgc attagccaag tatttccttc tagtggataa tccttattgc
    31681 tcgttgcagc gtcacttgga atgagtcctt gtttgtacca gctatgcatt gtacgtaaca
    31741 gtccgatata cgtttcatta tcgtattggt tgatgatttt cgaatcatct ccatccagat
    31801 caatagcaaa tggtaacgta ttccctaaag gataatcaaa gttcccttgg actttgaagc
    31861 cttgtccaat ggcaaaagct gccgtgtttg gttctttttc atggaattcc ttcaagactt
    31921 tttctgcatc agcgtaagat tggattccat caattgaaag atcgtattta tctagtaaag
    31981 ctttgttaaa agtaagtact tgttgagcaa agacatttcc attgactggg aaagcatata
    32041 attttccatt gaccagattt cctttaatat atgcttcatc taaatcatta taagcttttt
    32101 cagcgtactt aggcgctaat tcagtcaaat cagcaaaggc tcctttttgg gcattggaaa
    32161 catagttgtt cgcaaaggcg atatcataat tttccccgga agaaatgatg acgttcattt
    32221 tcttttcgta atcgccccag ccaatgtaat tgatattgac ttttacaccg atctcttttt
    32281 cgattcgttt gtttgctact tccatcaatt gatcgaaatt atctggttta tctccgattt
    32341 gatacatctg caaagtgtcc gaattgctag cttctgcctg gtctttagaa ccagaacctc
    32401 cgcatgctgc taatgttccc aacaacaacg ctgaaacacc tgtaaaggct agtttcttcc
    32461 ataacttcat ttttcttcct cctaatttta tgatttatat ttttgtttct ttattctttt
    32521 actccgccga ttgttagacc tttgacaaaa tgcttctgga aaaatggata gctgatcgca
    32581 ataggcaacg tcgagatgac tacgatcgcc attctggcag attctcctgg aactgaggct
    32641 aatccgcctg tcaattggct tcctgctcct gaattttgtg tcagatattg aatattactt
    32701 tggattttca tcaaaagata ctgtagtgga actagcttat catcttggat gtatagtaaa
    32761 gcattgaacc agtcattcca gtaacccaat gcggcaaata aactgattgt cgcaatacct
    32821 ggaacagcta acggtaaaac gatctgaaca aatattcgca gttcgctagc tccatcgatc
    32881 cgtgccgatt caataatcga atcagagacc gaacgtttaa agaatgtgcg catcacaata
    32941 atattgaagg gactgacagc cattggcaag atcaatgccc aaatcgtatc ttttaactga
    33001 agaagatttg tcataacaag ataattggcc accatccctg gtgagaacaa catcgtaatc
    33061 aaacaaaaaa cggtaaaaaa acgacggaat ggaaagtttg accgtgaaat cgcataagca
    33121 tataaagaag tagccgtgct attgatcagt gtacctaaaa cagtaacaag tacagtgacg
    33181 aaaagcgctt ggaaaatttt atcctgcatc tggtcgaaca agtacgtata accaaatata
    33241 ctgaactttt ctggccaaaa actgtacccg tgagtcgaca atatggactc atcggtaaaa
    33301 gagatggcga taacaaatat aaatggcaaa atacaggaaa tagcaaagag tgcaatcatt
    33361 atattagaga aaaaattagc cgtcttattg aaagagcgga cttggactct ctccactttc
    33421 ttttttttca tttttttcct ccttagaata atgcagaatc gctatcaaaa cgtcgggcga
    33481 tcgtatttgt gatcatcaac aagacaaagc cgacaactga ttggtaaagt cctgcagcag
    33541 ccgtcatacc aaaatctcct gtggatgtca ggccattgta gatatacgta tccaaaactt
    33601 gagtcacatc atataatgca ccggaattcc taggaatgtt ataaaacagg ccaaaatcag
    33661 ctcggaaaat gtttcctacg gctaaaatcg ttaaaatcgt cattagtgga ataagttgtg
    33721 gtatcgtcac atgtttgatc tgctgccatt tgctagctcc atcaaccata gcagcttcat
    33781 aataagtcgg atcgattccc ataacagatg caaagtaaat aatgctgtta taaccaatcc
    33841 ctttccatgt accaatgaac accaagataa acggccaata tttcggttca ttataccaat
    33901 tgacagcttc tcctccatgt ccagtgatcc actgattgaa tattcctttg tcaggactta
    33961 aaaaagcgta aacaaagtaa ctgataatga cccaagatag aaaataaggt agtaaggaca
    34021 tcgtttgata aactttaacg agtcgtttat ttctcaactc gctcataatg attgcaaata
    34081 cgactgagat cagcagattc aaaaggataa atgctacatt atataaaatt gtatttttag
    34141 tgatgagaaa tgcttgatcc gaagaaaata aaaatttgaa attttcccaa cccacccaag
    34201 gactttccct caagctagcc aagaacccat ctgctgaaat atgaaaattt ttgaatgcta
    34261 cgacattcgc cattacaggt atgtaaaaga aaaaaatcat ccaaagcata cccggtacag
    34321 ccatcaggac aagtgcttta taacgccata gattcataaa aaaatgattg aacccctttc
    34381 gttttttcat ctaaaaaccc ctttcgattc attcgcaacg taattatagc gctttcaata
    34441 atgagaataa ataaacaaag tatagggaaa atgaaacaaa gtataggaga ggttgtgaaa
    34501 tcagcgtttt ggcctgagta ttagagaaaa aaacgggaaa atagttcctc atattttgcc
    34561 gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaga ggttgtgaat
    34621 tcagcgtttt ggcctgagta ttagggagat cgtgtcctta ctatctaact ctgagtcaca
    34681 aggaacaatg gggactgtaa caaaagtctt gtcacagtcc cttattccga acaaacggtg
    34741 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaaagcca tccgaaagtc
    34801 agacggacaa gtctgctttt cagatggcgc tacaactact ctgcttattc ttttagctat
    34861 ccattcaatt atttttctct cacttgttct agctcttctt tggtcatggg tcgatattgc
    34921 cctttattca aatctttagg cagccaaaat tttcccatac ggattctttt taaatataag
    34981 acttcttttc ctactgcttt gaccattcgc ttaacttggt gaaactttcc ttcatgcaaa
    35041 atcagacgga taattgaagt ttctgtctct tgatctactt gatcaatttg gagttttgcc
    35101 ggcaatgtct gctcatcgcc atcaataatc agtccttctg caaataaacg gatatcttct
    35161 tctgtcatta taccgctaac ctcagctaga tattctttct ccacatgttt tttaggtgat
    35221 agtaactgat gggcaaacac cccgtcattc gttaaaatca agaggccctc tgtgtcttta
    35281 tctaatcggc cgactggaaa aagatcttcc cgataatcct ggtcttccaa taaatcgatt
    35341 acggtttgat catgattatc ctgagtagca gaaatgactc ctgcaggttt atgcaaaatg
    35401 tagtaaaaat ctttttgata cgtaatgatt tctcctaaat aagtaaccgc atcttcgaat
    35461 tcatttactt gcgctttatc gcttttcacc acttgtccat tgacttcgat cgttcctttt
    35521 ttgattaatc ctttgacttc tttgcggctg ccaagtccca tatctgctaa aaacttatct
    35581 aaacgcatgc gtttccccca tttcaaaaga gcatcaatct agctcttctg attcacttat
    35641 tttattttta atttatgtcg taaacgcgct attcctggtc ctagtagtct ttctgccaag
    35701 cggattttca aagctaaata accatacact aaagccccta tacctgctac tacaagaatc
    35761 aacaatagag actggaattt gctatcttcg tttaaaaaag caccaaaaat catctttgtg
    35821 atccacgaag ccaaaagcat aatcaaggta agaataaaga tcagcaatgt cctgcgccag
    35881 acaaaacttg gtttaaagcg ggctacttca tggattctct ttaagatcaa ggtacaagac
    35941 acagcaaatc cgatcattgt cgccaataaa gggccgtata cttcaaataa acgaatggct
    36001 gggtattgaa gaaggagttt cactagaaac ccaataacca aataattaat tgccgccttg
    36061 ttttcaaaca ttccttgcag catgttggaa actagcatat atagtcctag gaagagtcct
    36121 acaaaacttg cttggatcag tacattactt cctagagaat ccggtgtata aaataatgta
    36181 tttagcggat aggcaagtaa cataacacca aatgtcgccg gaaacataaa aaatgaaaac
    36241 aactgtaaat ttccgcttgt caattttgcc agacctactc gatctttcaa cgtaacagct
    36301 tctgtaatca acggtaaccc agtagacgcg atcgatgttg ccaatgcaat aactaccatc
    36361 gtcaacttat ctggattggc actaaaaatc gaaaaaagat ccagtaactg tgcattggaa
    36421 tactcggtgg atgcagacat gatcttcata aaagtgaact gatcgactaa tttgaagatc
    36481 gttacccctg aacccacaat aataaacgga atcgcttctt taacggtatc taacaacaat
    36541 tccttcgttg caatatttat tttgttttcg ctgtagtgaa taaacgccga tgtatacgct
    36601 tgatgtttct tcaaaaaata caaaaggaca agaatacttg ctagcatacc gataaatgcc
    36661 gcgaaagttg attgcgtgac cgccgtcaca tagtcaccat ccatgacttt catgatgatg
    36721 aatgtagcca atagcatata aaaaatacgt gccacctgtt ctacaatctg cgacagcgca
    36781 taaggcatca tttcttgatt gccttgcaaa taccctcgta tcacactcat acaaggaaaa
    36841 accaatacag cgatactcaa agaacgcatc gttggaatca gttcttctcc gccaccagaa
    36901 gcatgagcta gccaaggaga ggcgatgtac atgaacaaag caaacaacag gcctaacccg
    36961 cccatcatct gtaatgcacg gataaataat cgtcgtgaag tgccatattc attcaatgaa
    37021 ttatagcgtg cagtttgttt tgcaatcgca gcaggtattc ctgctgtaga gatcagtaga
    37081 aacaatgcat atatattgta tcccatattg aataatccat tggcagcctt agcattttcg
    37141 cccatccaag cataccaggg aatgatatag acagcgccta gcaatcttga aatgatatta
    37201 ctggcggtca tccaagcaga gcctcgtgcc attttttctt gcggtgttaa ttgatctaat
    37261 gctggttttc tttgattatc catcggtcag attccaactt tctacaaaac attactatca
    37321 ttttaattgg taaaacagaa actggcaatc gattttatat aacttttata agaacaattg
    37381 tttttttgaa ttcggtttcg tttttttggt aaagtaaaga aatatacagc cttttatggc
    37441 taacatttat aggagatatc aatgacacaa gaaataaaaa aactacccgt tatattttta
    37501 ggtcttttct ttatcacagt tggattgaat tggttcttgc tccctcacga tatagcttcc
    37561 gccggagtgg gcgccatcgg acatttagtt gaaacggcct tttctatcca acgctcagtt
    37621 actgtttgga gcatcaatct tatgatgctg cttctagccg ctgtcctatt aggaaaatca
    37681 gtatttttta agactgtagc gggaagtctg ctttttcctg ttgttttaga gattgttcca
    37741 aaagtggaaa tgatctcctc acatttcttt tcactgattt caggaagtct tttgttcagt
    37801 cttggggttt acacattgta cacagtcggg gcctcaaatg gcggtataac gataccgccg
    37861 attattttcc acaaattttt tcggcttcca atgccgcaag gacttttact taccaatagt
    37921 cttatcgtct ttttgaatta ttatgttttt ggattgttgg agacactatt cgttctccta
    37981 tctattacag tcagttcatt gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga
    38041 aaatttgaat aaagctggat tttgtcatag cggaaacaaa cgatatatta aaaagagtgt
    38101 gctataataa accaagaaat tgaataaagc gagggatcaa tcaatgagct tctctttaga
    38161 agacatccgt catttactat taaaagaaaa tctattaaaa gaatttgttt caacaagagg
    38221 ctggcatctt gatgtaccag aaaaagctga atttcttcag ttaagttatg attcaagaaa
    38281 agctgatgct tctactttgt ttttctgtaa aggaatgaat ttcaaagaag aatatttgga
    38341 ttcagcaatc gaacagggta ttcaatatta tgtatcagaa caaccatacg aaaattcagc
    38401 agtaggaatc atcgtaacgg atatccgtaa agcaatggcg cttcttgcta tggcttttta
    38461 cgactatccg caaaataaat tgaaagtgat cggattcact ggaacaaaag gtaaaacaac
    38521 tgctgcgtac tttacaaaag caatcttgga tcacacaaca aataaaaaaa cagcattgct
    38581 ttctactatg aatacaacgt tagatggcaa aacatatttc aaatcccatt tgacaacgcc
    38641 tgagtcatta gatttatatc gtatgatgtc agaagcagtt gaaaatggta tgactcatct
    38701 agtgatggaa gtttcttcac aagcttacaa aacacaacgc gtatacggct tgactttaga
    38761 tgtcggaatc ttcttgaata tctcgcctga ccatatcagt ccgatcgaac atccgacatt
    38821 tgatgactat ttctattgca agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa
    38881 tcatgaaagt gactattttg atttacttag ggaaacagcc gatctattcc agattccaac
    38941 gatcacttat ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag ataaaggtac
    39001 tcacggtttt actttatctt ctcatgaaga tcgcttagct atctgcgata ctgcttatga
    39061 tattttgctg gctggaggat tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc
    39121 actaacaggt gcctccagtc aagatgcaca agacggatta aaagaagctc gtgtaccagg
    39181 ccgaatggat caattgatcc aatccaatgg agcgcatgtc tatgtggact atgcacataa
    39241 ttatctaagc ttgaaaacat tgctgaaatt tgccaaaaat gaacatccag atggccgtgt
    39301 aattgttgta ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg attttggaaa
    39361 agtgctatca gaaacagctg acatcgcctt tttgacggca gatgacccag catttgaaga
    39421 tccgaaaaaa atcgcggaag aaatcaatga agcaattact aatccagatt tgatcgttca
    39481 ttatgaaatg gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga
    39541 ttcagtcgtt attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga
    39601 accttatgaa ggggattatg caatcgtgaa gcggttgatt gaagaataat ggtatttata
    39661 tactagtggt ttcccatttc taagaaaaaa agggactgtg attttttgtc acagttcctt
    39721 ttttcttaga aatcatttta acttttcact agctaattct gctgcctttt ctctttcttc
    39781 atttaataat ttattatcat agaatttgat aaatgggaac cagataatga atgcgatgat
    39841 tgcacaaatg actgctaaca cagcagcttt ccaatcacca ccactaccaa caaatgctcc
    39901 gatcccgact ggtgaaggcc acggcatctg tgcaagcatc ggtttgacga tttccagccg
    39961 aatggcaaaa taagcgagtg tagcagaagc catcggagct aagaaaaacg gaaccgctaa
    40021 atagggattg tagacgattg gcatcccgaa aatgattggt tcattgatat tgaataatcc
    40081 tggtacaagt gaggcttttc ctaacacttt cagctgatca gatttcgcga ggaatgcaat
    40141 aaagatgatc aatcctaaag tcgcacctga tccaccaacg gttacataag aattgttgaa
    40201 ttctcctgcc aacggataat ttgcgccttg tgcgttagct gccatattag ctaatacgat
    40261 cggggtcaag aaagaagtga tgatattcgc tccatggatc cctacgatcc acaatgcatg
    40321 gatcaagaaa taaatcacca tgacaccaag ccatgtattc gtcaactgcg taacaaaccc
    40381 aaatgggata gcaatcattt tgaagatatc cgttcctagt gcaactaata atccattgat
    40441 gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga gcagtgaaag aacgagaaac
    40501 tcctggcgga acaacatctg gcatttttat gacccaattt ctcttgacac acatacaata
    40561 caattcgacc gcaataatcg acataataat agctgtaaag attcctgaag ttcctaaacg
    40621 ttcgacaaaa gagcccatcc taataccatt tactacagtt tctgtctcat tgatagaagc
    40681 aatcaagctg attttaccat cagtcatgat caattcaggg atacacataa agaatgcaaa
    40741 aacagatagt aatgcccccg tgagcggatt aacatttaat ttttgttcat ctcgttcaat
    40801 tgacgttaat tcataagcaa acacaatggc aaagtaaata gataaaatcc ccatcgtcat
    40861 tgtattgacg atcatgtaca agtcactaac tttgaaaaaa gttgcattaa aaaatccttc
    40921 taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa aacatcgagc ctacaatcgt
    40981 gaaaggaata gaagccatcc ccgctgccat gacagcccga acgattttaa gttgcgcaat
    41041 tttccccata ggtcccatca ggtatttctg caaaaaatca aacattagtt attctccttt
    41101 cctaaatata acgctttttc atattgttta atacggatat actgttggtc ttggttgagt
    41161 gaaattttct ttaaacgata ctgtataaat gcactggcaa aaataccgct tacaacaaga
    41221 atgaaaacaa ataactgtcc tccattgtta tttgccataa aaggatacaa gaatgtaaat
    41281 cctgaggtaa acaacactgg aatcactaat aaattaaaga cactctggaa caaaaaatac
    41341 cactttgtcc atggaacgtt cgtttggtgt tttcgataaa gctttacttg ttctcctact
    41401 gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac ttcctaaact tttagctgat
    41461 agaagcagaa tcatccattg catgttcatg aaaaagaaaa aggcagtcag gtaacgtacg
    41521 attagataac gattgaaata catgtttttc aaactaagtt gctttttttc taattcgatt
    41581 ttccttttta ctgtatcctg cacaatgatc cttcctttct atttattttt ttgatacaaa
    41641 taattcattt cctgtgcaac ttccaataat gtcatcgtcg tcatcagatg atcttgtgca
    41701 tggaccatga taatttccat ttctattttt gtcccgctgg catattcttt cagaagttga
    41761 gtttgtgact gatgcgcatg aagaatctct tcatttgctt gtcgcaactt ctcttctact
    41821 tcttcaaaca tctctttacg catcgagcga aatgcttcat gtatcagtgt ccgtgcattt
    41881 ccgctatgga aaatgatatc aaaagaagtc acttgtagtg tttcactgtt tattttttca
    41941 gccatctgtt ttcctcctta ttcgatcagt ttgataaatt tactgctgaa attctcaaaa
    42001 ataggttcag ctaagatttc ctgctgtact tttagctggt caactaacct aacgattgct
    42061 ttagtcacga ccgtaattcc ttcattttcg acataagaag gcgaaactaa aaaaacaaaa
    42121 tgaatttctt gatattgatc ccactgcatc ccagaaggaa tcaatgcaac ggctaccttt
    42181 gtttcgattc caactggaac agcgggatgt gggactgcca ctgtatcact gaatatgatt
    42241 tgtcccatat tttcacgttg ctggatctgc tttcgcattt ctcttatata tccttcttct
    42301 tcatttatgc tcagcttttg cagcaaatca tgaagaattt cttccttttt cggttcgtac
    42361 gtataagtct tgaaaaattt ttcagacaac tgttcggtgt aaatctgctt tttttgctgt
    42421 agagaaaatg ctggtatcgg tctatctgag gaatgatagg gtatagattc tccaactacc
    42481 tttcgtattt tacgaacatc atcttcattc aaaaaaatac tgacatgtaa aactggaatt
    42541 ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca ggtcgattcc ttttaaagtt
    42601 gtttcattga tctcataata gccttttaca cttaccactg taatattttt accgaattca
    42661 ctgactacgc gattctttaa tagttgggca ctgccgtaac ccgttgcaca gataatcaat
    42721 gcatgtagtt tatgcgcagc tctttctttt tccaatgccg ccatgagatg caaagccaaa
    42781 taggcccatt catcttcatt gatggtatag tttttcaaag ccggcatgtt gctgaaatgc
    42841 ctttttactg cgtcaaagac tttgggattt tctttcttta tctcctcagt caatgggttc
    42901 tcaatgcgaa tacctcggct caatcgaatc atcatcggtg acaaatgatc gagtagtcca
    42961 ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt cctgagacaa tcttttcaga
    43021 actttcgcta attcctttga tagttcctga tcattttgat tttctttgtg attcgactta
    43081 gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg gaaacgatac acctgcgatt
    43141 acctcaatac gttgaacgat ccgctgcgcc acttgatact ctactcgctt agaaacttct
    43201 tgttcgatgc ctaattcctt gatttcaaac ccttctcgga gccgtttgat ccccaaagcc
    43261 aagtgtaaag tcagattctg tatgataaaa tccgaaactt ttaacttagc ttctcttgtt
    43321 tcatcgagaa tgataatgac tagctcttca aaactgatgt ctgggaaaaa acggctttgc
    43381 cctaaatatt ctttcaatga atttccataa tggtttccaa aaaaagtatc catgataaaa
    43441 tgccgtcgat ttctttcttc cccttcgatc catgtccctt gaccatgctt agaaacgatt
    43501 tttagtgaat atggaatcaa tcgctccttg atttcttgca tgtcctttgt caaactggaa
    43561 cgactgatat aaagctcttc tgccaaatca tccataaaaa gaatggcatc ttccaaaagc
    43621 aattggttca atatatactt ttgtcgatct tcggactcat aaaatccttg cggttctcta
    43681 gcatatccag gtactatttc ttgccgactc aaaaacaagt caaatgtcag tctttgtaaa
    43741 atatgcagtt ggtagccata acctggttta gcaatgatct tacctccgtt tttttctact
    43801 aactctttga tccgctgcaa gtaattacgg attgtccgat cagataataa caattcagaa
    43861 gctaaacgtt gactggtgac atactccttc ttgtgatcaa tcaaatatct caacagcaat
    43921 ttttccttct gtttcattat catccactcc tattccatca tatgttctct tcgatcacct
    43981 ctgctaactt ctggatgcct gtagggattg ggacatacgc ttggaaaggg atactaacaa
    44041 caggctttcc aacagattct cctaattttt tgaatttatc caaaaacatc gtcgtttgcg
    44101 ggctgatcag aaataaatca aaatcactat tcttaatcat attatcgcct tctgtagctg
    44161 aaactgcatc tacgagtacc tcttctcttt tttgcttaaa gaaatccgtt gtctttgagg
    44221 ccatcatcga ggatgacatt cctgccgcac aaataatcaa tgcttttttc ataaatcaat
    44281 cactcctttt atttttataa accaagcata tcttgtaagc gttttctatt caaaatatat
    44341 atttccgtag cattgcggaa aaaatgtgta tcatatcttg aataataatt tctggcaccg
    44401 tgatatacta gtgatagaat tttttcagga ggggatttag tgagtatcac agtgaatatt
    44461 ttgtatacaa gtacaaatgg acaagccaga aaatttgcag aagaaatggt cgaaagaaac
    44521 attgtcaaag cgattcgtgc agaagaagga aacgagaaat atgactatta ctttccacta
    44581 gaagatccag aatctctttt actgatcgac cgttggaaag atgaagaagc aatcgaaaag
    44641 catcacaaat ccgaaatgat ggctcaaata aacgaactcc gtaaaaagta taaattaaaa
    44701 atgaaagtcg aacgtttcac tgatttaccg taaaaagata cacatggaat gatagcaatt
    44761 tatcatccat gtgtatcttc actttttcct tattttttat tcgatctcat ctaatgattc
    44821 aaaccgtgtt tttctcttat ttttcggata agtatttcag tcaccccaat atacatgatt
    44881 cctaaacata ccccaaggat attctcaaaa atgcggatcg ttactgctcc tggaaccccg
    44941 aataaactgg cagtgattgc caaagcacca aaagaattaa aaactgtttt atatctatag
    45001 gtagaacaaa cacctaaagc tagaccacct aatataccga cccagttaaa aggaataaat
    45061 tggctgatac caataaataa aacacatcca atcaatgagc caatgattcg gtctactgct
    45121 cgttctttga aaccaatcga catcaatcca tagctagata caatagaaga aaatgcaaac
    45181 cctgcccaca tcaatcgctg aaaagggaca tactctcctg caacgatcaa aagactaatg
    45241 ccaaatgcat agatgagcat ccaaattttt tcctgcgaat acatgccttt caaaaaaatc
    45301 tctttaaaca agcttttgtc tttgttcttt tctcgatgtt ttctgtaaag gagaactgaa
    45361 aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg taaagaaatt cttgttcaac
    45421 gaatctttag gtaacgaata aacaataaag agatatgaaa agccgtacaa agaagcattc
    45481 cccattttgg gatcactagc ggttgccatt aatatgctgc ttagtaaaat aaaatgaatc
    45541 aacatgccca taactgacca tttgatcagc tggacatacg gtgctactaa tagtgagaac
    45601 atgacgattg ctaaacctat gatcgaatgg gaaatcttat aaccaaagtc aacaaaacga
    45661 atactcatca acatacagaa taaaaccaca gcgtatggtt tatgcttcga ttcaaataat
    45721 gttgtaatta taatgataaa acaaatggca aacaatacga tgaataatga tcgtaaaaac
    45781 aatgaacaaa tatacatgca cttttctttt ttagaagctg cttctcgtat tttttgtttt
    45841 aaaataaacg gatctagttg tagagactga taaaaatcca cctgttgtcg tcctttcttt
    45901 ttacttttat tttttacagt catctatgac aggttgtaaa tactatggaa catcatatgg
    45961 cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt tttataaaaa aacatatcgt
    46021 tttcttgtat gattaaatca ccacaaaata aacaactgag agaacgatat gctttatgaa
    46081 tggttctatc aaaaaaattc tgagatcaat agacaaaaat ttgatgatta cagaggtctc
    46141 ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct gcagttctct tccctgctcc
    46201 atgtgtctgt agaaattgtg gttctgctgt ggtagatgga aataggaaat taatggtggt
    46261 gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa tataatcata tgccaatggt
    46321 tatacgccta aaaaagcagc gctatacctg taaaaactgt cgaagccatt ggaacgctca
    46381 aagttatttt atccgtccaa ggcactcgat ttcaaatcat gtcagacata aaatcacttc
    46441 tttactgact gaaaaagtgt cgttattttt tatttcgaaa agttgttagg tatctttgac
    46501 aaccgttatt cgcacattga aagagttgag aagctattta ccaaagtctt ccaaaagaat
    46561 tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg tttccgttga ggataaactg
    46621 agttttattt ccgcggatgg cgagacagaa aaattaatcg agaaaattta cctacacaaa
    46681 aaattcctcg attaacaaac tatttcctta gctgcactaa tcctaaagaa atagaattct
    46741 tagttacaga catgaacgcc acctactttc agctcaccaa acatgttcta ccaaatgcga
    46801 aattggtgat tgatcgatta cttggttttt ccacgccttt caaggaggcg taacctttat
    46861 tcacgagttg gttgaagctt tccgagacaa aagacccgac ttgtttttct ccttattgac
    46921 aaagagccat taaaactaga gaaaaaatta caactgatcg ctgattattt gtagaaagaa
    46981 aatcaaagta ttacccaata aacttgagtc attaggcaaa aaacactgcg aaactcaaga
    47041 atgattttcg cagtgttttt gtatattttt tattaactag tattactaca aaggtttatt
    47101 tttcacttct caacgcagca caaaattgtc tttacttatt tactggttgg gttcaacctt
    47161 ctctactccg atactaatat ttgtttgtat accccttcta aaatcggtag tttccattca
    47221 attgatgggt actagttgat tttaaaaact atgcttattc aattttttat tttagctcat
    47281 atatttcgtt tctttctaaa ttttatactg atgaaaacaa gtagcacacc caatattgac
    47341 aatataaact ggctaatgat ggtttctcca gtttttggaa gatgcttatt tccactgctt
    47401 gttggattgt ttcctcttat atctgagcta tcagcaactt ttctattctc ttttgttaaa
    47461 aagatcgcca cttcttgttc tttttcgatc ttgaaagtta ctggtgtcgc atccaattca
    47521 taccccgttg gcgcttgagt ttctaccagc tgatatgctc ctggtgctaa gccatctatc
    47581 gccagttttc catctgctcc tgtggttaga cctgactgca gaacggttcc attggcatct
    47641 tgtaactcaa aaacagctcc ttgcaggact tctccgcttt ggtcatcgat ctttgtcaac
    47701 acgacaccgc ctggtgtaag tcgattttct ttcgtcaatt ctaccaccgc tgtctgactt
    47761 ctttcgatct caaactcgat tggtgtcgca tccaattcat accccgttgg cgcttgggtt
    47821 tctaccagct gatacactcc tggtgctaag ccgtcaatcg ccagttttcc atctgctcct
    47881 gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa aacagctcct
    47941 tgcaggactt ccccgctttg gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt
    48001 cggtttgctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc aaactcgatc
    48061 ggtgtcgcat ctaattcata ccccgttggc gcttgagttt ctaccagctg atatgctcct
    48121 ggtgctaaac cgtcaattgc cagttttcca tcttctcctg tagttagacc ggtttgtagc
    48181 gtttctcctt ctcggttctg taactcaaaa acagctcctt gca
//
LOCUS       DAJSVF010000014        47036 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSVF010000014 DAJSVF010000000
VERSION     DAJSVF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.014"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(144..1013)
                     /locus_tag="ON550_000762"
     CDS             complement(144..1013)
                     /locus_tag="ON550_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3953869.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1033..2154)
                     /locus_tag="ON550_000763"
     CDS             complement(1033..2154)
                     /locus_tag="ON550_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR3953870.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2156..2551)
                     /locus_tag="ON550_000764"
     CDS             complement(2156..2551)
                     /locus_tag="ON550_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3953871.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2570..3376)
                     /locus_tag="ON550_000765"
     CDS             complement(2570..3376)
                     /locus_tag="ON550_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3953872.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3369..4157)
                     /locus_tag="ON550_000766"
     CDS             complement(3369..4157)
                     /locus_tag="ON550_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3953873.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4171..4647)
                     /locus_tag="ON550_000767"
     CDS             complement(4171..4647)
                     /locus_tag="ON550_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3953874.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4719..7376)
                     /locus_tag="ON550_000768"
     CDS             complement(4719..7376)
                     /locus_tag="ON550_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3953875.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(7395..7799)
                     /locus_tag="ON550_000769"
     CDS             complement(7395..7799)
                     /locus_tag="ON550_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCR3953876.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(8031..8288)
                     /locus_tag="ON550_000770"
     CDS             complement(8031..8288)
                     /locus_tag="ON550_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR3953877.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(8413..9345)
                     /locus_tag="ON550_000771"
     CDS             complement(8413..9345)
                     /locus_tag="ON550_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953878.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            complement(9751..10506)
                     /locus_tag="ON550_000772"
     CDS             complement(9751..10506)
                     /locus_tag="ON550_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3953879.1"
                     /translation="MDFEQNVRLNFDLLSDNEKDMVFFIRTHKAQVVHMSIVELGEQL
                     LSSKSSVLRLAKKLGFRGYSEMKYSLEQSIVQSVIPPTDLIALLKDEINRTFQLADQT
                     NFQPILEQLKAAKTVVVYATGFTQNNFSKDFVNDLILSGRPAMLISGETNFEMLSHTL
                     TKDDFVIVTSLSGETPSIKSTIKNLNMNRIPLCGVTELGKNFLSEHSDFLLYYETREL
                     PSNLIEGSRSMIGLNILLSILSRKYREFILFDE"
     gene            complement(10613..12283)
                     /locus_tag="ON550_000773"
     CDS             complement(10613..12283)
                     /locus_tag="ON550_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3953880.1"
                     /translation="MNEWWKSSVVYQVYPQSFKDSNNDGIGDLMGIVEKLPYLAKLGV
                     DVIWLTPIFDSPLIDNGYDIRDYEKISENYGTLEDFKRLLEQAHQLNIRVILDLVVNH
                     TSNQHAWFIESQKNKENPYSDYYIWKDPKEDGSAPTNWGSTFGGSAWEYVESRKQYYL
                     HCFAKEQPDLNWENPKVRQSIYKMMKKWFDLGIDGFRMDVISLISKHPDYPDAPNSMP
                     YTKSYYVGASNGPRVHEFLQEMNREVLSKYDSLTVGETPNTNSRQAQLYTFPERKELN
                     MVFHFDHMHLDYGKYGKFSDVRFKLSDLRATLSEWQEALSDGWNSLYWSNHDQPRAVT
                     RFGDENEYRIESSKMLGTLLHMMKGTPFIFQGEEIGMKNVPFSSIDEYQDIETNYFYK
                     KMKEEGESDEYIKRAIYLKSRDNARTPMPWDHSKGYGFSNSDPWISFSPDNQQINVED
                     ALKNPNSVFYHYQKLISLRKNYPIIQHGTYKLINENDSAVFGYIREYKNELLVVICSF
                     SKESVPYELPKSLQGRKGVLLLSNYEQSPSELEDKFILRPYEALVYHI"
     gene            complement(12287..12706)
                     /locus_tag="ON550_000774"
     CDS             complement(12287..12706)
                     /locus_tag="ON550_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR3953881.1"
                     /translation="MKKIIFASHHNLAVGLKDTVQYILPNVGEITAIAGYIDNSSIEE
                     KINEVMVRDNEDTDILIFTDMLGGSVNQAFIKYINQPNVYLITGMNLPVVLTILLNTL
                     NKRLTDNHIREAIQEAQDQLVYVNDYLKNIVVDEEDE"
     gene            complement(12719..13528)
                     /locus_tag="ON550_000775"
     CDS             complement(12719..13528)
                     /locus_tag="ON550_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3953882.1"
                     /translation="MNNEGNKLSKKDIRRAAFRYMFMACNIFNYENQQGPAVVYGLEK
                     ALRKIYPNDEDYRASLNNHFKYFNTTTWMANLLLGASLAMEEKDGVSSLDAVQSFKTG
                     MMGPLAGVGDTLVWVLYPTIMGSIAAYMGLEGNPTGAIIWLCLNLFFLFFRFKLFSIG
                     YTSGLKLVTALGSKLSIFTESASIMGLTVVGALIPSVVKMNVAVVFQSGKVKLPIQTE
                     ILDKIMPALLPAVLTIIVYKLIASKKMTVIQIIFSVIIFALILSFFGILKV"
     gene            complement(13521..14321)
                     /locus_tag="ON550_000776"
     CDS             complement(13521..14321)
                     /locus_tag="ON550_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3953883.1"
                     /translation="MEIIQGILIIILAVWVVLDQQGLVITTWFPIMIALFAGLIMGDM
                     TTAMVIGGTFQLMALGVANIGGSSVPNWGLAALVGIYVAIRTTNNIEEAKAVALAVGV
                     PVGMLGIQLDVLAKILNTYVTHAAQKAANKMDFKKMNQILWLGPVIFGLSTAIPTALC
                     VVFGDRIVRLILDFVPKWFTDGLSIAGSMLPVVGIALLLQVMPVKKYMTMLLIGFVLS
                     AYLNMPILGISIVGFALAYYFFTTEIKKAETASPVAANIVDEGDDFDE"
     gene            complement(14336..14812)
                     /locus_tag="ON550_000777"
     CDS             complement(14336..14812)
                     /locus_tag="ON550_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3953884.1"
                     /translation="MGLVLARIDQRLIHGIVVTQWAGATKAKRLMVVDDQVSKDDTQK
                     AAMRMSKPAGTGMSIIDTDTAIINFKLGKYDSHNVLMIVKEPSTLVKLAENGIDIPKV
                     NIGIMFDGEGKQTVKKMVSVDEQEIADLKKLEAMGIPVTFHFVPSDPEEPLDVRIK"
     gene            complement(15207..15686)
                     /gene="rlmH"
                     /locus_tag="ON550_000778"
     CDS             complement(15207..15686)
                     /gene="rlmH"
                     /locus_tag="ON550_000778"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR3953885.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16114..17400
                     /locus_tag="ON550_000779"
     CDS             16114..17400
                     /locus_tag="ON550_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR3953886.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTTSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSGF
                     GGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKDG
                     TKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANS
                     VTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKI
                     ASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQE
                     QILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKV
                     GDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17439..18044)
                     /locus_tag="ON550_000780"
     CDS             complement(17439..18044)
                     /locus_tag="ON550_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR3953887.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLGADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWSGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(18249..18713)
                     /locus_tag="ON550_000781"
     CDS             complement(18249..18713)
                     /locus_tag="ON550_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3953888.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18735..19055)
                     /locus_tag="ON550_000782"
     CDS             complement(18735..19055)
                     /locus_tag="ON550_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3953889.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(19198..20277)
                     /gene="pepA"
                     /locus_tag="ON550_000783"
     CDS             complement(19198..20277)
                     /gene="pepA"
                     /locus_tag="ON550_000783"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR3953890.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20477..20827
                     /locus_tag="ON550_000784"
     CDS             20477..20827
                     /locus_tag="ON550_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3953891.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            21050..21997
                     /locus_tag="ON550_000785"
     CDS             21050..21997
                     /locus_tag="ON550_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3953892.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22054..23472)
                     /gene="pepV"
                     /locus_tag="ON550_000786"
     CDS             complement(22054..23472)
                     /gene="pepV"
                     /locus_tag="ON550_000786"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR3953893.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            23621..24466
                     /locus_tag="ON550_000787"
     CDS             23621..24466
                     /locus_tag="ON550_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR3953894.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            24470..24910
                     /locus_tag="ON550_000788"
     CDS             24470..24910
                     /locus_tag="ON550_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR3953895.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            24960..25154
                     /locus_tag="ON550_000789"
     CDS             24960..25154
                     /locus_tag="ON550_000789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953896.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            25290..25976
                     /locus_tag="ON550_000790"
     CDS             25290..25976
                     /locus_tag="ON550_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3953897.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(26383..27072)
                     /locus_tag="ON550_000791"
     CDS             complement(26383..27072)
                     /locus_tag="ON550_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953898.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(27047..29176)
                     /locus_tag="ON550_000792"
     CDS             complement(27047..29176)
                     /locus_tag="ON550_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR3953899.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     PGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(29224..30561)
                     /locus_tag="ON550_000793"
     CDS             complement(29224..30561)
                     /locus_tag="ON550_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR3953900.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGLAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(30813..31172)
                     /gene="rplT"
                     /locus_tag="ON550_000794"
     CDS             complement(30813..31172)
                     /gene="rplT"
                     /locus_tag="ON550_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR3953901.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKDALAK"
     gene            complement(31225..31425)
                     /gene="rpmI"
                     /locus_tag="ON550_000795"
     CDS             complement(31225..31425)
                     /gene="rpmI"
                     /locus_tag="ON550_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR3953902.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(31497..32021)
                     /gene="infC"
                     /locus_tag="ON550_000796"
     CDS             complement(31497..32021)
                     /gene="infC"
                     /locus_tag="ON550_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR3953903.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(32065..32190)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32341..32973
                     /locus_tag="ON550_000797"
     CDS             32341..32973
                     /locus_tag="ON550_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3953904.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(32975..33880)
                     /locus_tag="ON550_000798"
     CDS             complement(32975..33880)
                     /locus_tag="ON550_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3953905.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASHILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(33965..34624)
                     /locus_tag="ON550_000799"
     CDS             complement(33965..34624)
                     /locus_tag="ON550_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3953906.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            34940..35884
                     /gene="mvk"
                     /locus_tag="ON550_000800"
     CDS             34940..35884
                     /gene="mvk"
                     /locus_tag="ON550_000800"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR3953907.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            35877..36854
                     /gene="mvaD"
                     /locus_tag="ON550_000801"
     CDS             35877..36854
                     /gene="mvaD"
                     /locus_tag="ON550_000801"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR3953908.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            36867..37952
                     /locus_tag="ON550_000802"
     CDS             36867..37952
                     /locus_tag="ON550_000802"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR3953909.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            37949..39013
                     /locus_tag="ON550_000803"
     CDS             37949..39013
                     /locus_tag="ON550_000803"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR3953910.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLASLGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(39262..40065)
                     /locus_tag="ON550_000804"
     CDS             complement(39262..40065)
                     /locus_tag="ON550_000804"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR3953911.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGATIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(40085..40687)
                     /gene="kduI"
                     /locus_tag="ON550_000805"
                     /pseudo
     CDS             complement(40085..40687)
                     /gene="kduI"
                     /locus_tag="ON550_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(40670..41374)
                     /locus_tag="ON550_000806"
                     /pseudo
     CDS             complement(40670..41374)
                     /locus_tag="ON550_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(41390..41869)
                     /locus_tag="ON550_000807"
     CDS             complement(41390..41869)
                     /locus_tag="ON550_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR3953912.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLSGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSIPVSGILIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(41875..42903)
                     /locus_tag="ON550_000808"
     CDS             complement(41875..42903)
                     /locus_tag="ON550_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR3953913.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDTFKNDPAYKDLY
                     QKIQEQGA"
     gene            complement(42920..44026)
                     /locus_tag="ON550_000809"
     CDS             complement(42920..44026)
                     /locus_tag="ON550_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3953914.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(44255..46852)
                     /gene="adhE"
                     /locus_tag="ON550_000810"
                     /gene_synonym="adhC"
     CDS             complement(44255..46852)
                     /gene="adhE"
                     /locus_tag="ON550_000810"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR3953915.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIICDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        14027 a         9760 c         8108 g        15141 t
ORIGIN      
        1 ggataatttt tctcttaaaa aatatcatta tttgtggtat ggagctgggt atcatgagtg
       61 aatgatattg ctgaatacaa atagtccgac cacattaagt tgatcggact tcataagcat
      121 tttattgact aatacttttt tgcttaagaa ttgattctaa catcttttgc taaagttaag
      181 catcctatag ttcctgcatt atctgctaac ttaggtgtga caatgtattc ttcgatattt
      241 ggcgtatcga catagccgtt aattagttct aaaaaacgtc tttgtacttt ctttttcaga
      301 tgttcttgtt gcatgacacc accaccaaaa attataatat ctggtgaaag catcagggtc
      361 gtattacagg cacattgtgc aatataatcc gcctcgattt cccagaacgg atcttctata
      421 gaaagatctt gtcccttggt tcccgtacgc atctcgatag cgggtccaga tgccaaccct
      481 tcgagacaat catgatgaaa aggacaagta ccagagaaag tatctcctga atgtctctta
      541 actaacgtat gccccatttc tggatggcta aacccttcaa caaaaatccc atcttgtata
      601 gctcctccac caattccagt tccaacagtg taataaacaa cactggataa accttttcca
      661 tgacctgaaa catactcccc gtaacaagca gcattcacat cggttgtcca ggcaattgga
      721 atttcgaaat gcttattcaa cgttccaaca aaatcaaaat ttttccaggc agtttttgga
      781 gtagaggtga tatgtccata ggtctttgat tcacgatgga tatcaatcgg tccaaatgac
      841 cctactccaa tgctttctaa ttgttcttta tagtgattaa agaaatcaat taccaaactc
      901 attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt ctaaaacctt taaatcatca
      961 ttgccaatgg cacaaacaaa tttagtccca cctgcttcta ttgagccata catttcttac
     1021 tccaccgtcc ttttatctag gaattcgttc ttccctgtct aatagttttt cgaaaaactc
     1081 atgtggttcc ttttggtacc aataggcaac agaagaaata tcgtcttgac gctcataaag
     1141 cttgatgtca tcatttccta gggtttggaa agtaacacgt aaattttctt tgaaactaat
     1201 ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt aaagcatcat cgccaaaacc
     1261 atgaactgga tttggattag gttttccagc actaaaataa tcccgggtgt gatctctttt
     1321 agattgaaaa ggatagccca taaatagcgt attaaaattt tgagcatatg ccctttctcc
     1381 aaagttcttt ttatggaaag cccaagcgcc gccaaaataa tcttctgctc ctgtagatgt
     1441 tattgtaggg aaatcttcat ctccatctat ataaaactta aattctcctt ccccccacca
     1501 gtatctttcc aatgcgcaca aagcaaagta ggttcctaca taatacccag tgcctttgat
     1561 tccatcaata agggtgtaat cttttccttt tgtcgtgatg ggttcacggt tccatttagc
     1621 atgaaaatac atcgcatcat ccggtacttc agtcaatgca taattaaatg tataaaaaat
     1681 gtgcttaata aactcaggat gttcattcgt taaagtaatc ttagcttttt ttcgaaatgg
     1741 catctcaaaa tagcaattca tccctgatgt tggattcact acgattggta aggaattcac
     1801 ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct aatggacttt ctacagaagg
     1861 agtggattca tcatcccaat aaatacgcaa aattaaatca cgtaagacga aactcccagc
     1921 gattgttttg tctgtcactg tcatccaaaa atgacgtaaa attccagggc cttccatttc
     1981 tgctaaagta attgtctctc cagcatttat cggaacagaa ggtgaccctt ttctcccaat
     2041 tcctagatga ctcgctgtca ttgccgcttt ccctttttca ccatttttat tttctggtga
     2101 aatagttcta ctttcctctc ccttgtggag ccatatatta tttaaacaat tcattttatt
     2161 cgtcctctat cctttcttca cttttttgat tattcatata gatgatttgt tgttgtgcct
     2221 ccgcaataat tgctccaata tctaaattat catttcctaa ctgcaaagcg attgacattg
     2281 ccaagggaag attcattcct gcaattacgt gcactctgtc agaaatatag gtaaaaaact
     2341 tttgatttac acttcctcca gccatgtcag ttaaaacaat tatttcgtct gtagtatcca
     2401 ctaactcttt aatttgttca tctatatcat caatttcttc gacatatgca cataagctac
     2461 taatttgatt atgcatacct gttaaaaact ctagtgtctc tttaaaaccc tcagctagta
     2521 ttgaatggct agcaatgatc aatcttctca taattcaaat ccttttcctt tacgctaata
     2581 ttccaaatgc agccataacc atcgcaaaaa caataacgag tagaatcaaa atggtcatac
     2641 tgattttttt cttactgagt agataatatg ctgcagcagt aataattact ggaattaatg
     2701 ttggtaaaat tttgtccaac attggttgga ttttcatact aacttcacca aaagtgaatt
     2761 taagtggcgt tgaaacactg ataactgaag caattaatga tccaactact gttagcccca
     2821 atactgaagc agcttcagtt aaataattaa tcttttcacc gtattcagta acaatcttca
     2881 ttccctgctt ataaccaaat tcaaagcctc tcattcggaa aaagaagatt accatgatta
     2941 aaatgaacca taaaaagatt cctacaggat tattttctaa agccatataa gccgcaattg
     3001 atcccatgat ggttgggatc atcgtcatca aaagagagtc cccaatacca gcaattggac
     3061 ccatcgtacc aactttaaag tcattaatcg catcttttgc ttctaatcct tgcttttcct
     3121 ccattgcaac acaagcacct aaaataatgg ctgccagcca aggctgtgta ttgtaatact
     3181 gaaagtgatt atttaaagct tcgatgtatt catcatcatc tttgtataat tttcttaaaa
     3241 taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt ttgataatta aaggtacaaa
     3301 ctgacatcaa ccaacgatag ttcgctttcc taagatcctt tttcgttact tgatattttt
     3361 cgctcatttt attcatcctc cccaataaac ccaacatcag aagctcccgc agaagcagtg
     3421 atttgtgcat ctttttcagc tctatgatac acattaaatg ctaaagcgaa tcctatgatc
     3481 gccgctccaa taataggtac cttcaaaaaa gcggctaaga aaaatccaac aagtaaataa
     3541 ctaatgtatc gacctgtagg cataaagcgt aacaacatcg caataccaac agcaggcaac
     3601 atttttccgg ctaatgttag cccatccata aaccaattag gtaaaacatc caaaacgtaa
     3661 ttaacagcag actgtcctaa agtaacggca ataaatactg gaattgctgc agataaacca
     3721 aagaaaattg gccccaacca caaaacacta ttcattttct taaattctcg tttattcgca
     3781 tattcctgtg atttgcgaac aacaaaacca tttaagattt tgacaattac atctagttgc
     3841 acccctaaca tacctacagg tagtcctatt gctaatccgg cttctactcc aagctggcta
     3901 ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg gtacagatga gccaccaaaa
     3961 ccggcaactc ctaatgacat caattgaact gtaccgccaa ctatcaaacc tgtttttata
     4021 tcacccatca aaattcctgt gaataatccc caaaaaactg cgtaatttac aaagatcatc
     4081 ggaatcccat aataatcaac atgtttaata aatgctaata tagtcagcat aataatttga
     4141 aaaatagtaa aatgcattat ccttactcct ttatcctaat tttgattgaa tttcctgtgc
     4201 cttatccgcc ggaacaaatt gtgtaacaat ttctacacca gaatccaaca attttttata
     4261 cgtgctgata ttttcatctg atgcataggc tctagaagat agctgtaatg cattatcttt
     4321 aacaacagta ccgccaacaa ttagtcggtc aattctaata ccgttatcta ctaaatgaag
     4381 gattgtttcc ggctctttca cgatgacaaa tactttttcc tgatcgtatt tccccttttt
     4441 aaaattcgca atcgctgttt cttcagtgat aattgataca gccattccag caggtctagc
     4501 taaacgcata cttgattttt ggatgtcgtt atttgcgaca gaatcatcga tcaccatcac
     4561 tctgtttgct ttagacattg gtgcccattg cgtgacaata atcccatgca ataaacggtt
     4621 atcaactctc gacataacaa tactcataaa tacttcctcc tttatctttc attttcttac
     4681 aagcaattac catgccaaaa caaaaagaac ttgataaatc aagctctttt tacataatca
     4741 taaacgtatt taacctcatg aatcgggatg tcgacactat aataatgttc aacgacactg
     4801 aaagattgct taaaatgtgt ctgaaatttt tgaaattcct cactcgtttc attgaaataa
     4861 atttcatctt ccaaagtatt ttgttttact aatcgttcaa ttaagcaact aatgtgaaca
     4921 tataatccga aacacgtatt attatcaaat gaaattccta gatcattttg taagatgtat
     4981 atagcatctg aaacccgttc taataattta gtcggattta atatggtcaa ttgctccatt
     5041 aaattcgtta gactaaaatt cttcagaata ttattgttca aaactttcag ttcattatca
     5101 ttaaagtgtc tattcaattg attggagaat ttacttatat tcattcctaa aatcatatct
     5161 tcaattgaaa agaattctac atcttctaga tgtggattta gtgttccaac aataaaaaga
     5221 atattgttct ctttacgaag ctccgataaa tagttttcat tcactaaaga gtaatagtca
     5281 cacgtttcta tgacaagatc agtatccctt ggaaagctat cttcaagtat cttttttaat
     5341 ttaaacgaag ttcctaatcc gcttgcacaa gaacagacaa tcatatcctt cttaatattt
     5401 gatgattcat aaatataatc cacattttga ttttctttta cttgttcaag aatctgttta
     5461 agcggtaaat ctaataatat ctgatttgca acatcaatag ccattcttgt tgaaacattg
     5521 tttagtagtc cgaatttttt ttgagcgtct agttccaaat ttagataaat tttctccagt
     5581 gaacccatat caacaagtaa aataatttct tcatatccgg tacactcttg taagtatcta
     5641 ttaattttgt ctgcaatctc actacttgaa gtcattagtg gcatatcaat ggcgtcgtat
     5701 atatatctct caacgatcct attagcagta cttgctatac aggttgccgt tagatctcca
     5761 tgagccaaaa tcagtgctaa acgtttttgc tgaaattctt tactacacat taacttaaat
     5821 aaaataacca aaacattttt ttgaattttt gttaaataaa tatcccctat ttgaataaaa
     5881 cgattgacta attgaacttc tcgactacac caactattaa tatcattgat gaaggaatat
     5941 aatttatcgt ccgatgttaa ttggatttga taaatccaag aattcaaaaa gatagacgtt
     6001 atttgaagat aataatagtc aaattctatt tgaaaatctc tctctatttc cttaacatac
     6061 tgtctaaatc gaatctcttg ataggtggga tatgttaaag ctgaattatt ttggatatca
     6121 acattctttt gtctaaaata tctatcatat ttctcaaaaa gctgctgctt atcagtgtct
     6181 ttcttccacg tttgataaag atactctata agttcgttga tattaaaaac ttcgtctact
     6241 ttttccaaat catccatttg aatatatttt aattcattct gtcttttcat aaccttcatg
     6301 ccgttgaaat tcacccattg tggtaaatga tacatctgaa tataaagttc ttccaaatca
     6361 tctaagaaat aaaaggcatt ggcacaggtc aattgaatga tatttttcaa attgccaaca
     6421 ttttcactaa agcgatagtt taataaaaaa tctaataatt gttgagatat cagtattttc
     6481 tttgctagtc tttcttcttc tgatttaaaa aatatcttga ttaactgttt tctttcataa
     6541 agagaacgac tgtctaatgt tggaagtgtt agctttacag gtattcttct caacaaagtt
     6601 gataatagtt tatcctcagg tggttctgat gttgcaaata taatccagca acttgatgtg
     6661 taccagtttt cattatcacc catccgatga aatttcctcg tatccataaa ctgaaaaagt
     6721 ttctcctgac attcattgct caatgcatga atttcatcta aaaataagac tccattatta
     6781 gctaattctg ctaaaccctt tcttccatct tcggctcctg tatatgcacc ttttacatgc
     6841 ccaaaaaaat tggacaagaa aagttctgga ttattggcat attcactaca attaactgaa
     6901 atgaaagatg ccttctcttc tatataatta ttggccaaca aatccttata cacataattt
     6961 gctaaatagc tttttccagt tccagtctcg ccatgaagta aaatgggtaa gccatttggc
     7021 ggatagtaga ttgctgcttt aatttgagca attacatttc gcagacttcc ttgacttccg
     7081 ataacccttt ctattccaac cctaaaagag ctatcatacc atttttccgc ttcctctgaa
     7141 gagctaaaaa tttgttgatt tggaactttc tccggtaaag aaaagaagat tactggacga
     7201 gtattgattt ttccaaattc tccatctttt acgaattcat tcaaatattg agagacaata
     7261 ttacgactaa catttaattt ttcagatatt ttctgtgctg tattgttccc ccatttattt
     7321 ttaatctcag agttctctat gacataattg acaatgcgtt cttttaattc ttccatcaca
     7381 gcacctcctc gacactattc gaattttgcc gacagtattt ttccataaat atgacttgct
     7441 aaaaaggctt tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag
     7501 acgttgaaaa gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc
     7561 agatcttctt ttgattcttc atctgaaagt aattcacgat acaacatcgt ttgtttgctt
     7621 actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg
     7681 tcggataaat atttatagaa aatcagcccc aataaataat ttttatattc tgacgcatcc
     7741 attttactac ggagattatc tgccgcacta aataacttct gatttaattc tgcacccaaa
     7801 aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactca
     7861 gttttagaaa aagcaaaaaa ataaagaaac ttattcttga taacaagcta cgtgacgtat
     7921 atccctaaca caaaaatagc catatccttt ggtttggata cggctatttg atattactca
     7981 ttcaattatc aaatcacagt aggtgtgaat ctcatcggga tcggcaatat ctacttctga
     8041 ttaggatcaa aggtgatttt ctcaataaag gtgtgataca aagctttaac ttgcttcttc
     8101 tcactctgga taagaaagca atccaaacta gtcaatatct gttggacatc tttgacttga
     8161 atcggttccc cttgttgttg aaagatccct aagatttcat tctctcgaat ttgtaactgt
     8221 cttcgttttt cagtcaattc atttaaacga tcttttaaca tcggaaacaa ttcgggacta
     8281 tcttccaaag cagcttcctg tttttgctct aatagttaga ttttgcgtac cattttcttc
     8341 gtttattttc cttcgattgc tgattttaat atagtctaaa ggaaatttat atttattgta
     8401 ttctttataa cttcaagaga acttcgtttg agtattactg tattttttta attccataag
     8461 aaaattagtt attacctgcc acctaatcag caaatctgaa cctgagtcaa tataattaat
     8521 attattttcc atctccaaca ttgtaagtac tgttggtgtt aatataggga ctttgtacaa
     8581 ttgtattttt ttgattttat catctgcaca aaggagattt tttaaatcta atccttcttt
     8641 tatttcaaaa gttgaatttt ttaaatctat aagagtaaaa ccaattcctt catactgaat
     8701 ttctgaaaat cgaatttttc tcccagttga tagatcaagg ggcataaaac cttttattac
     8761 attgtgagtg tcaccatcat ttacaatgtc attaggaatt ttgattttag aattctgtgg
     8821 agcacctatt ggaagataat aattcattat aattttgtag tcttcatgag gaaataaagt
     8881 acttttagga ttgctaagat attctcgtac cgaatctaag aatggcttat ccattgaatc
     8941 ttcatcccca tttaaactaa aaaacattgc tacaattgct tttgcaactc taagtgaatt
     9001 cattttcttt attgaatgtg ttaaatcaga cttaccattc tttaaacttg tattgttata
     9061 aaggaaattt ttattgtctt ctgcaaatct aatatattcc tcaacatact cactaccaaa
     9121 aaaactatta catttactac ataacgtata ataaacattt ccttgaggtt tccttttaat
     9181 ccccttcgga aaatcacctt ttgtaaaatc gctcttaagg aaatcatcta aattaccagt
     9241 tctggtcttt ttacctccag tattccctga ttttttaggt ggtacatgtt ctttagtaag
     9301 ttcagtgtat ttttcacata aaatacactt acccatcttt ttcatatcta tccccactct
     9361 ttttaattct tatatacaat tatatttcaa atattcatta ataaatatcc ctatttatga
     9421 taaggaccta cataactttc cccctagata ttacgaatat ccagggggtg ttattgcatg
     9481 cgtaacagcc tatgctaatt tacaacatag atcgaagtgg cttagctcta aaaaataagc
     9541 cgcatctttg tcagatacga ctgtttagca ttgctcattc aattagttga tgattttttc
     9601 atcaaatttc agatatagtt gaatatttcc tttgttcagc ttatcaaatg tcactttatc
     9661 aatgaatgtt cgatagattt ccttgttctc actcttgaaa cgattggcta acaacttttc
     9721 aacacttgct cctttgtttc gaaatggagg ctactcatca aaaagaataa attcacggta
     9781 ctttctagag agtattgata gcaaaatatt taatccaatc attgaacgac ttccttcaat
     9841 taaattgctt ggaagctctc ttgtctcata atataacaaa aagtcggaat gctcgctgag
     9901 gaaatttttt cccagctcag taactccgca aagtggaatt cggttcatat tcaaattttt
     9961 gattgttgat ttgatgcttg gtgtctcacc gcttagagac gtaacgatca caaaatcatc
    10021 ttttgtcaaa gtatggctta acatttcaaa gtttgtttca cctgaaatta acatggctgg
    10081 tcgtcccgat aaaatcaaat cgtttacaaa gtcttttgaa aagttatttt gcgtaaagcc
    10141 tgtcgcatag acaactactg tttttgccgc ttttagctgt tctaatatcg gttggaaatt
    10201 tgtttggtcc gctaattgaa aagttcggtt gatttcatct ttcaatagtg cgataagatc
    10261 tgtcggtgga ataaccgatt gaacgatgct ctgttcaaga gaatatttca tttcagaata
    10321 cccacgaaat cccaattttt ttgctaaacg taagacagaa cttttagaag acaataattg
    10381 ttctcccaat tctacaatgg acatgtgaac tacttgagct ttatgcgtac gaataaaaaa
    10441 aaccatatct ttttcattat ctgagagcaa atcaaagttt agtcgaacat tttgttcaaa
    10501 atccatttcc tcacctctca tatatacaga aggaatttcg gataaaactt gcgctgtttt
    10561 atccgaaatt ctttcttatt gcccaaattc taacacagtt tttacgtgct tgctatatat
    10621 gataaacaag tgcttcgtaa ggtcgtaata taaacttatc ttccaattca gatggtgatt
    10681 gctcataatt tgatagaaga agaacccctt ttcgaccttg caatgattta ggtaattcat
    10741 agggaacaga ctcttttgaa aaagaacaga tcaccactaa tagttcattt ttatactcac
    10801 gtatataacc aaacactgct gaatcattct cattgatcaa tttatacgta ccatgctgaa
    10861 tgattggata gtttttacgc agtgaaatca gtttttgata atgataaaaa actgaatttg
    10921 gattttttaa agcatcttct acattgattt gttgattatc tggcgaaaaa gagatccaag
    10981 gatcactgtt cgagaatcca tacccttttg aatgatccca aggcattggt gttcgtgcgt
    11041 tatcacgaga tttcaaataa atagcacgtt tgatgtactc atcactctcg ccttcttctt
    11101 tcatcttttt ataaaaatag tttgtttcaa tgtcttgata ctcatcaatt gaagaaaatg
    11161 gaacattttt cattccaatt tcttcccctt gaaaaataaa tggggttcct ttcatcatgt
    11221 gaagtagtgt tcctaacatc ttactagact ctatccgata ttcgttttca tcaccaaatc
    11281 tagtaacagc tcgcggttga tcatggttac tccagtaaag agaattccaa ccatctgaaa
    11341 gcgcttcttg ccactcactt aacgtagctc ttaaatcgct aagcttaaac cttacatctg
    11401 aaaactttcc atactttcca taatcaagat gcatatgatc aaaatgaaaa accatattta
    11461 attctttacg ctcaggaaac gtataaagct gtgcctgtct gctatttgtg ttcggtgttt
    11521 caccgacagt taagctgtca tatttactca aaacttctcg attcatctct tgtaaaaatt
    11581 catgcactcg cggaccgttg gatgctccga cataataaga ctttgtatag ggcatgctat
    11641 ttggagcatc aggataatca ggatgcttgc taatcaagct gataacatcc attcggaatc
    11701 catcaattcc aagatcaaac cattttttca tcattttata aatagactga cgaactttcg
    11761 ggttctccca gtttaaatct ggttgttctt ttgcaaaaca atgtaagtaa tactgctttc
    11821 tactctcaac atactcccaa gccgacccgc caaatgtcga tccccaattc gtaggtgcac
    11881 tcccatcttc tttgggatct ttccaaatat aataatcaga atatggattt tctttatttt
    11941 tttgtgattc tataaaccaa gcgtgctgat tacttgtatg attgacgaca aggtctaaaa
    12001 taactcgaat attcagttga tgcgcctgtt ccaataatct cttaaaatct tctaacgttc
    12061 cataattttc tgaaattttc tcatagtctc gaatatcata tccgttatca attagcggag
    12121 agtcaaaaat tggagttagc cagataacat caacccctaa ctttgctaaa taaggtaatt
    12181 tttcaacgat ccccataaga tctccgatac catcattgtt tgaatccttg aaactttgag
    12241 gatacacttg gtagacaaca ctacttttcc accattcatt catccgttac tcatcctcct
    12301 catcaacgac tatatttttt aagtaatcat tgacataaac caattggtct tgtgcttctt
    12361 ggattgcctc acggatgtgg ttgtctgtca atcttttgtt taatgtattc aataaaattg
    12421 tcaataccac aggtagattc atcccggtaa tcagatagac attaggttga ttgatatatt
    12481 ttataaatgc ttgattgact gaaccaccta gcatatcagt aaaaatcaat atatcagtat
    12541 cctcattgtc tcttaccatt acttcgttaa tcttttcttc tatagatgag ttatcaatat
    12601 aacctgcgat tgcagttatt tcaccaacat tcggtaaaat atattggaca gtatctttca
    12661 atcctacagc taaattatga tgactagcaa aaataatttt tttcattctt tattctcctt
    12721 aaacctttaa aataccgaaa aatgatagaa tcaatgcgaa tatgatcact gaaaaaataa
    12781 tttgaatgac cgtcattttt tttgaagcta tcaatttata aacaataatt gttaaaactg
    12841 caggtaagag tgctggcatg attttatcaa gaatttctgt ttgaataggt aattttactt
    12901 ttccagattg aaaaaccact gcaacattca tttttacaac agatggaatc aaagctccaa
    12961 cgactgtcaa acccataatt gaagcagatt ctgtaaatat cgataattta cttcctaaag
    13021 cagtcaccaa ctttaaccct gaagtatacc caatcgaaaa aagtttaaat cggaagaaca
    13081 aaaagaatag attcaaacat aaccaaataa tggcaccagt cggatttcct tctagaccca
    13141 tataagctgc aatcgagccc ataatcgttg gataaaggac ccaaaccaac gtgtctccca
    13201 caccagctaa aggacccatc atcccagtct taaaactttg aactgcatct aatgaactga
    13261 ctccatcttt ttcttccatt gctaagctgg cacctaataa taaattcgcc atccaagtcg
    13321 ttgtattgaa atatttaaaa tggttattca gagaagctct gtagtcttca tcatttggat
    13381 aaatttttct tagtgctttt tcaagtccat aaacaactgc cggaccttgt tgattttcgt
    13441 aattgaagat attacatgcc ataaacatat agcgaaatgc tgctctacgt atatctttct
    13501 ttgataattt atttccttca ttattcatca aaatcatctc cttcatcgac tatattggca
    13561 gctacaggtg atgccgtctc tgcttttttt atttctgtcg tgaaaaagta atacgctaaa
    13621 gcaaagccga cgattgaaat tcccaaaata ggcatgttta aataggcgga aagtacaaaa
    13681 ccaatcaaga gcatcgtcat atattttttc accggcataa cttgtagaag taatgctatt
    13741 ccgactacag gcaacatgct tccagcaatc gataatccat ctgtaaacca cttaggcaca
    13801 aaatctaaaa tcaatcgaac aattctatca ccaaaaacaa cacacaatgc agttggaatg
    13861 gctgtagata atccgaaaat cactggtcct aaccacaaaa tctgattcat tttcttaaaa
    13921 tccattttat ttgctgcctt ttgtgccgca tgagtaacat atgtgttcaa tattttagcc
    13981 aacacatcta attgaattcc cagcatccca acaggaacac ctactgctaa tgcaacagct
    14041 tttgcttctt caatattatt tgtggtacgg atggctacgt agatacctac aagagcagct
    14101 aaaccccagt taggaacgga cgatccccca atattcgcta cacccaaagc cattaattga
    14161 aacgtaccgc caataaccat tgcggttgtc atatctccca taatcagccc tgcaaacaaa
    14221 gcaatcatga tgggaaacca tgttgtaatg accaatcctt gttgatctaa tacaacccac
    14281 actgctaaaa taataattaa aattccttgg ataatttcca ttccaattat ctcctttatt
    14341 ttattctcac atctaatggt tcttcaggat ctgaaggaac aaaatgaaaa gtaactggta
    14401 ttcccatagc ctctaatttt tttaaatcag caatttcttg ttcatcaact gaaaccattt
    14461 tttttactgt ttgtttccct tcaccatcaa acatgatccc gatattcact tttggtatat
    14521 cgataccgtt ctcagctagt tttactaatg tactcggttc ttttacgatc ataagcacat
    14581 tatgactgtc atacttgcca agtttgaaat taataatagc tgtatctgta tcaataattg
    14641 acatccccgt tccagctggc ttactcattc gcattgctgc tttttgtgtg tcatccttac
    14701 tgacttgatc atctacgacc atcaatcgtt ttgcttttgt cgcacctgcc cactgtgtta
    14761 ccacaatacc gtgaatcaat cgttgatcga ttcttgctaa tactaatccc atatctatcg
    14821 tctccttttt tgttatcgtt tacatcccaa agtatatcta cgccttgtta ctttaacaat
    14881 agttttagaa gtatcaggga aacagtcgtt atcttaggaa atagtttctt tattaagaga
    14941 aatttcccac aaaaaaatag cccaaccatc gattagatga ctgagctccc taaatttacg
    15001 ctatctttta ttactattta cttctttttt atcgcaatcc aaatttcgcc atcacaatcc
    15061 gtcaactctt ttgagaaatc agtaaaggag agttctgatg cttgaaccaa ctcatattct
    15121 gaagttggca accactccgc gtatattgac ccactgacat ccctaaaaaa ggtgcgaggt
    15181 ggagaatatt tttgatacaa ctatccttat ttatgatatg gagcccccgc attgatccgg
    15241 aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt
    15301 tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag
    15361 ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattgatc gatttgttta
    15421 gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt
    15481 tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct
    15541 tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag
    15601 gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt
    15661 cctactgaga taattttgat attcatgttt tctctccttt ggtcttcacg ctcactttgt
    15721 ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt
    15781 ccccaacagt tatgcacaac atccacaggt ttatacacag aaaacagtgt tattttacct
    15841 tatttcatca cattcattag gcaaacatac gttttataag aatttatcca ctttatacac
    15901 aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt
    15961 atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt
    16021 ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca
    16081 gtagataaat aaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag
    16141 atgaaaaaaa ataaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg
    16201 atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat
    16261 agttctacta caagtggcaa tcaaaattct gctggtgaaa cagttgtgga aaacgtcaaa
    16321 gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc tgtcgtttct
    16381 gttatcaact tacaaagcca aaaccaatca agcggttttg gcggactatt cgggcagcaa
    16441 gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg aagtggcgtg
    16501 atctacaaaa aagatggaaa tactgcttat gtagtcacaa acaaccacgt agtagatgga
    16561 cagcaaggtc tagaagtctt gatgaaagac ggtacgaagg taaaagctga attagttgga
    16621 acagatgctt attcagattt agcggtgttg aagatcaatt cagataaagt cgaaacggtt
    16681 gcctcttttg gtgactcaag tgctctgaag gttggtgaac ctgcgattgc gattggttcc
    16741 ccattaggat ctgaatatgc gaattctgtc acttcaggaa tcatttcttc cttgaaccga
    16801 caagtaacaa gtacaaatga atcgaacgaa acagtcaata tcaatgcgat ccaaacagat
    16861 gcagcaatca accctggaaa ttctggtggt cctttagtga atattgaagg acaagttatc
    16921 ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaacgt aagcgtagaa
    16981 ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca actggaaaaa
    17041 gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc tgccgtttct
    17101 actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg tgtcatcgtg
    17161 acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata cgacgtcatt
    17221 actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc tttgtataag
    17281 aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga aaaaacaact
    17341 actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa
    17401 taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa tgcttctccg
    17461 actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt tggcaaatgc
    17521 aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt tcctgaccag
    17581 atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt ttgtccagct
    17641 tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac ttctgtctca
    17701 gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta cccgacaacg
    17761 atcttcggtt catgatcagc tccaagctgt tcttcgaatc ctgcttcttt gatcaatcga
    17821 ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc gattgttaga
    17881 tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc tttgatccga
    17941 gcaacattcc atttgcgttc aacggtgttt tctgttcctt tatcgtcggc tatgataacc
    18001 atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt tcctccatta
    18061 tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgagat ttttttcatg
    18121 ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg gactgtgaca
    18181 agaattttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt cgtacaagtt
    18241 ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac atcacaaggt
    18301 gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac tgcatttaga
    18361 cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt gactgccatg
    18421 gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc aacagatttc
    18481 ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg atcgactaga
    18541 ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat tacgtttgct
    18601 acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa agcagcaagg
    18661 gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt catcttgacc
    18721 cctcctaaca gatgtcagcc tttcgttaca ctttctagga acgattcgat ttcctgtttt
    18781 gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat cacgacaaaa
    18841 cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc acggtctact
    18901 tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg tttgataaaa
    18961 cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt ttctacgtag
    19021 cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact ttctgtttat
    19081 gtatttttca attaaattat acacctttct ttcgaactaa caaacattga tcctcttttt
    19141 ataaaaaaaa tctggcaaaa gcaatctttt gccagatttt atagtttcac tatcgcatta
    19201 ttttccgtat acgatcgtat tcactgttga tttatccaaa cctttcagtg tttgaatcaa
    19261 catttctcgt gcagcttcaa aatcgcgaat actgaacatc gtttgatgcg tatggatata
    19321 acgtcccact acgccaatca ctgtacttgg gattccttcg ttttgtgtat gagcagcacc
    19381 tgcatctgta ccgccttttg aaacaaagta ttgataaggg atattgtttg tttcagcgat
    19441 atccagcaag tactcgcgca aacgaggaag catgatcaaa cctggatctt ggatacgcat
    19501 caatgtacct tctcctaaat gtccgaatgt gccatttttc gtcactgtat catctgctgc
    19561 agagcagtct actgcaaaga acagatctgg cttaaattta ttgacagaag ctttagaacc
    19621 acgcaagcct acttcttcct ggacattcgc gcctgcaatc aatgtatgac ctaatgtttc
    19681 attttgcaac gcttctaaag cttctaatac cattgtacag ccatagcggt tgtcccatga
    19741 tttgctgatg atattctttc cgttggctgt tttgatcgtt tcagcgtaag gaacgattgt
    19801 gtctcctggg aggacaccgt aactcattgc ctcttctctt gattcaaacc cagcatcgaa
    19861 caaaatatcg gttacttcta ctgatttttg tccgcttgtt cctcgcaata aatgcggggg
    19921 aacggaagaa gaaatacatg gataattctt accagtagag gttttaagtg taaaacgctg
    19981 tgcagaaaca acgtaaggat tccagccgcc tagaggtacg actttaaaaa gcccgttttc
    20041 ttggatttgt gtcagcatga atccgacttc atccatatgt gctgcgacca ttacacgggg
    20101 tgcatccgct tcttcatgat ggcgcagacc aaaaatgccg cccaaaccat ctaactgtaa
    20161 ctcatctacc agcggttcca tatgactttc catataagcg cgaacatctt gttcaaaacc
    20221 gcttgttcct tgtaattctg ttaactcttt gattcgttga aatgtttttt cttccatatg
    20281 ctattcgtct aaatttcaga ctaaactcct ctcagactta tattgatctg cggataaaat
    20341 atgatttttt ctgcagaccg atcgtattta ttatagctta ttttttctgg tagcggttat
    20401 ttatttcaaa gaaaatatga gagtgtggta aaataagcac aaggagtaac aagtgagaag
    20461 aaggaggtat gtccacatgt atgaagaaag cgaactaagc tattttaaag gcgggttagc
    20521 agttggaatc ggtctaggat tattgagcgg ggtagcttca acgatttggt atcatcgcaa
    20581 aaaagtattg tcagcagacc aagtacttga aaatatcaag tcatcttttc taaaagaagg
    20641 tgcaatcgaa ggttcttgga tctcttttga aaaggaacct attaggaaat tcgccgttcg
    20701 gtcgagtgct tatcggggag gtctttctcg aatagaagat ggcgaaactg tttattatga
    20761 atttttagca gatgctcata caggaaccgt attagaaatt tcaagaaaaa aaggtgcaac
    20821 agattaatac gtcaaaaaag tgagagaacg aatacgcacc cactattcaa aagagaggta
    20881 tcatgtcctt cttttgtagt gggtggtttt ttagatagag ctttccaaac atatatacca
    20941 gtattttctt tttaaataaa tgatatttag cgggaaaaac tagcatttcc tttgattatg
    21001 aggtattttt aatttagctt ttgttttcta tagttaagga gagtgaaaaa tggaacaaca
    21061 agaaaaaatt gctgttctga ttccttgtta caatgaggaa gcaacaattt caacagtcat
    21121 tgcagacttt aaacgagaat taccagaagc agatatttat gtctatgata ataattcgac
    21181 cgatcgaaca tacgaattgg ccgttcaagg tggagcaatc gtaaaaaaag aacctcgtca
    21241 aggaaaagga aatgtgatcc gccaaatgtt ctttgatatc gatgccgact attatttaat
    21301 ggtagatggc gatgatactt atcctgcaga agctgtacat ggtttgctcg agaaattacg
    21361 ttctggtgaa gcagatatgg tcattggtga tcgcctatca aatggaacct actttgatga
    21421 gaataagaga cctttccacg attttggaaa taaccttgtt agaaatacaa ttactagaat
    21481 gtataaaaca aaaattcttg atgtcatgac cggctatcga ggattcaatc gtatctttgt
    21541 caaaagtttt cctattatga gttctggttt tcaaatcgaa acggaattga cgatccatgc
    21601 tttagataaa aaattcaagt tcgttgaaat cccaatcgat tatcgtgatc gtcctgaagg
    21661 cagcgaatca aaattgaata cattctctga cggattcaaa gtgatcatga tgattattaa
    21721 aatgtttaaa gattataagc ctttgctttt ctttgggatt tggaccatct tcttcttttt
    21781 atttggattg gtcacgggga ttcctgtgat tcgcgaatat atgctgacaa gctttattac
    21841 acgcatccct tctgcgattt taagtactgg attgatgact ttagcacttc tttcattagt
    21901 aacagggttg attttggata cagtcgtcac gaacgctaag aaagaatatg aattgaatct
    21961 atatcatgtt tatcatgaat accacagtag aaagtaaaaa aaggatcaag actgcaaaaa
    22021 taatttgcaa ttcttgatcc tctttttaat taattatttc gccaaacgat agatggcatc
    22081 tgcatagatt gctgttgctc tgaacaagtc atcaagactc ataaattcgt ttgcttgatg
    22141 cattgtatca gtatatcctg ggaacatcgc tccataagca acgccacgtt tcaacaaacg
    22201 tccgtaagtt ccgccaccga tgatttgttc gtagcctttt tctccagtgt ggtcttcata
    22261 gacttgcaat aatgtagcca ccaatggatc atccattggt acgtaatgtg gttccattac
    22321 gcgagcacct cgagtaactg ttgcgccttc ttgccccact gtttgttcga tgtcactttg
    22381 caatccgtct actgttacac cttttggaaa acggaaattc atattgatga agttcgcttc
    22441 ctctgtacca ttttcttcaa aagcaaatag tccagcattc attgtcaatt tgcccatttt
    22501 ttcatcttca taagcgactc ctagtttttc tccataaaaa tcttcatgga cataagcagc
    22561 tgctacatgg ataaattgtt tagcagatcc taggaattca tagttatcta agaacaatgc
    22621 caagaaagaa cctgcattga taccagattg tgggcttgca ccatgtgcgc ctttaccaac
    22681 taattcgatt tttacatacg tattgtctgc ttcaatcgtt ccgcttactg gatttgcttc
    22741 gatgaattgg taaaatgctt cttccatcgc gatagctgca tctgcacttg gtacttgaag
    22801 tgctgcagtt gctgttcctg gcaccatatt ttcgcgaagt ccggaaacaa aagatttcaa
    22861 gacataatct cctgcattat cgcctttgaa tcgtaaagct aaagaaacat ttcctttttc
    22921 accattgatg atcggaaatt cagcatctgg tgagaaacca aaatctggct cttcttcatg
    22981 ttcgaaataa taagccatat cgccccagcc gctttcttca tcgctgccga ctacgaagcg
    23041 aacttttttt gatagtttca agtcaagttc tttaatgatc ttcatcgcat agtaagctgc
    23101 catacttggg cctttgtcat cgcttgatcc gcgtgcaaag atctttccat ccttaattac
    23161 tggttcataa ggatctgtgt cccagccatc tcctgcagga acgacatcca tgtgtccaaa
    23221 tatccctagc gtttcgtctc cttcgccaag atcaatatgc cctgcatagt tatcgacatt
    23281 ttttacttta aatccatcac gttcgccata agctaacata tgtttcaatg cgtcgcgagg
    23341 tccaggtcca aatggtgcat ctggtgttac ttttgagtca tcgcgctcgc tgttgacacg
    23401 taaaagattt ttcaagtctt caagcaagtc ttctttacgt gcttctactt ctttttgcca
    23461 atcaattgtc ataaagttaa tcctccactt atttaagtct tcgtgttcat gataccacaa
    23521 attacagaaa aaaatagttc aaaaaatctg acaattcctt ttcttatctg tttttctcta
    23581 aaaaaaatat acgctataat gagtaaaagg aggtttttcg atgctgataa acgaacaact
    23641 agtgaaaaga gtcatcacac ctcgtccaaa aatttcacat aaaggaacat ttggacgtat
    23701 cgttttgatt ggcggcaatg aacaatatgg cggagcaatc attatgagtg ctttagctgc
    23761 cgtacacagc ggagcgggtc tcacgactgt agtaactgat gaaaaaaatc ggagtgctct
    23821 tcacagtcat ctgccagaag caatgattgt agattggacg gatactgacc agtttctttc
    23881 cgtaataaag caagcagatg tactcttgat tggtcccgga ttgggtactt cttctaagag
    23941 tttggaacgt ttcaaacaag tactagaaaa ccaatcaaaa gaacaatggc tggtgattga
    24001 tggctctgct ttaagtttac ttgctgctaa tcatcttttg ctaccctatc ccgaacaaac
    24061 tgtacttaca cctcatcaaa tggagtggca acggttgagt tctctaccga tacaagaaca
    24121 acgggataaa gaaaacaaaa agaagcaaca ggaaatcggt gctgtgctcg ttttgaaaag
    24181 tcatgagaca aaaatttatg gtcaccagca cttctacacc aatcctttag gttctgctgc
    24241 tatggcaaca ggcggcatgg gtgacacttt agctgggatg attacaggat ttttagccca
    24301 gtttcctcaa aatgaccaaa cgattgcagc agctgtttat cttcatagtt tcattggaga
    24361 ccagctggca aaagaacaat atattgtttt gccgacacaa atcagcgaag cacttccgaa
    24421 gtggatgaat tatttttgta aaaaatcaat ggaaaacgag gcatgagtca tggagaaatt
    24481 tacaggttat gtgcgagaac ctttttatta tgaaacagat caaatgggga ttattcatca
    24541 cagcaattat atccgctggt ttgaagaggc tcgggtagcc ttattggtac atctagggtt
    24601 tccctataaa aaaatcgaag aatctggtat catcatcccc gttctggaag taaattgcag
    24661 ctataaagaa atgatccatt acggagaaaa agtgagcatc catccaacta tccaaaagta
    24721 tacggggaca cggttggatt ttagctacga aattgtggga actgaagaca aaaaattaaa
    24781 aactactggc agtagtaaac actgtttttt actttctgat tcgcagcgat tagtgaagct
    24841 gagcaaagtc aatccggagt tagatcagtt attccaagcg tacgcttctt tggatgatgt
    24901 aaattcttaa taaagaccgt ctaagcattt ttcttgtatt gtgtcgagta gttcgtataa
    24961 tgaaaagaaa aaagcaggtg atccccatgt atgtcgttaa agtaatgcac ggttacatcg
    25021 ataaaactgg ctgtcgcact cgggaaaaaa acttagacaa tctgctcatt ttcaaagaca
    25081 aaaaagaatc cgaagctttt gctaaaagaa tcggcggtcg tgtcaaaccg atccaagagg
    25141 tacgaccaga ttgattcaat atttttcttt tcgaggctct aataggcatg cctatcaggg
    25201 ctttttttgc agactgtgtt tttatgccca cacgatttgt aagcgattta tgttataata
    25261 gattatatct gcatagaaag gaaggttaaa tggttatcaa taatttgcaa cagcaagctt
    25321 atgaatctat acgtaaacaa atcatttatt ccgagttagc acctggaagt aaaatctccg
    25381 ataaagtgtt agaagaacaa ctagctatcg ggcgtacacc gattcgagaa gcactgatcc
    25441 aactgcgcaa tcaagagctt gtaaaaacca ttccacagtc cggcacttat gtttcaaaaa
    25501 tcgatatccg atctgcttca ttagcccgtt atacgcggga aaagttggaa aaaccgatcc
    25561 tgcaagaatg cagtgcgaaa atgaccaagc aagatcaagc taaactagaa aaaatactag
    25621 aacaaaccga tcaggcagtg atcgctcaaa ataaaaaagc tttttttgat ttggatactg
    25681 attttcaccg aacttgttat gaaattgctg gaaaacgaga aatttgggat tggctagaaa
    25741 gttatagtac gcacttgaac cgtttcagat ggttacgttt gacgatttca gaattagact
    25801 ggggtcgagt attagatgaa catcagacaa tgcttcaatc aatgattgat cataactttg
    25861 atgaagtcgg atttctttgc acgatgcatt tacatatgat tatcgaggag caagaatacg
    25921 tgattcataa ttaccccgat tattttgaag atattcagga cagacccatc aaataagagg
    25981 ttttagaatc agcgttttga cctgagtatt agagaaagaa acggcaaaat agttcctcat
    26041 attttgccgt tttttttgag ctaataccga gggtcagctg attgcacacc gtttattgag
    26101 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aatagcttct
    26161 tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgttttcc
    26221 aagaggtcgt gagcctgccg ttcatctccg agtcacaagg aacaatttca aaaaacagct
    26281 tctcatgttt gttgaatatt ttgacttgtg accgaggagt tgacaggaga acaccgttta
    26341 ttcggaaaaa aggactgtga cacgactttt gtcacagtct ctttatttgt aatctaaatc
    26401 atactccgta catctttttt ctatttcttt tatttcttct aagatccctt ggctacgaat
    26461 ttcattatat ggccaaagta agactgcctc taatacataa ggcgaaaaat cgctttgatt
    26521 catctctttt gaaaaactca tcgctgctat ctcgctcaat gcacgaatgt ttgattgatg
    26581 tgtataaaaa ggcaaccgcc acagcacaat tttttcgctt tgcgtataaa ttcccttaac
    26641 agaagcttcg atgtggtgaa atagttcatg tccaataatc aaagcttgcg ctgtttcttt
    26701 agagatcgaa gctagtcctg aacattttaa tttttctaac ggctccgtca tcaatcggat
    26761 cccttcatct tctgcataag ttgcaaaagt gattcgttcg tttatttgct ctgattcaca
    26821 ggaaacgatt ggaacagcca tttgttcaca aatttctcct acatcggcaa aagggtactt
    26881 ttttttgatg ctacatgccg tttcgatacc acaatcgatt gattgttgta taagaatttt
    26941 ctgcttggac aaaggaatct ttttccaaaa taaatctcgc cgaaactggt agaatcccca
    27001 aatttcttcg gggatctgtt gtaattcgtt tagtaactct tctaacttat gtttgtttga
    27061 tatttccggc aacgacgatc acttcttcct ccggttccat agtagatact tccagatcca
    27121 tcaaaagctg tagttgttcc agatccgtcg ctgtaaaatc agagactctt gctcctaagc
    27181 agagataaaa ttctggtcgg gcaatcaatt cagtttgata aactgccata tcttcccaaa
    27241 gttgttcgac agctgtcata taattggctg cagttgtttt catacatgaa gcatgtccac
    27301 gttggacctt gataaatcct ttttgatcga tgatccgaag acttccagca ttttttgaac
    27361 gattctgttc gccgcataca taaaagaaag gtgtattttc caataagcaa acttcatttt
    27421 tatttaatcg catatcctct gcagcaagct ctagtgcttc ttctgttgtg atctccttgg
    27481 ttagatcggt tgctttcact tccgtagaac cagtagcgat agccgttact tttgaagtct
    27541 gtggatcgat ttctacgtgg acttcgatcg attctggtgt agcgcctgat tcaatcgctt
    27601 tattcatcgc ttcatttttc aacgatcgta tatcttcttt acttggcgaa gggatgatac
    27661 gttctacaac atctcggacc attgcaagtg ctacaccaat agaagaaatc acttcagcgt
    27721 tttcaggaat cgaatatttc acacccattt tattactaaa gtaagtaatc aatgaggcag
    27781 cgccgccccc tacacctaca agagaaatct gatctttctc caagtgatac ttgtctgcca
    27841 attctaaaat caccggttca atcttagcat aggatttttc catgatctgc tctgcgatgt
    27901 cttcaaccgt cgtatgacaa tagtctgcta atgcctgtat ggcttttcgt gcagatggga
    27961 catttccata agaaaaatgt tcttcttgca ctaatccagg gacattggct gcacatgtat
    28021 ttgtaatagt cacttcttct ccgtcttcca tcactacctt gacgtaatca gcagggtctc
    28081 cttctttagg agagaaaaat tcaaccttag gacctttgat tttttctgtc tccgtaaata
    28141 ctgcataatc tagaccggca atatgtgcag aacgtggacc gacatcgatg attcctgact
    28201 gattagcccg taccatcgaa ccgcctgcca cacctagaac ccgaacatct aatgaagaga
    28261 tatatgttgg gtggccacct acaattgaat aatcgattgc tggacgtcca tttttaatga
    28321 ccccgatatt ggttgttgtt ccacctactt caaaataaac accattagac gctctcaagt
    28381 acatcaaaga gcccatgact gatgcagcag gaccggaaag catggttaaa acaggtcgtt
    28441 ttttcatttc attgatttcc attactccgc cgtctccgcg cataatcatc aaagaaacat
    28501 tgacccctgc ttctcgtacg gaatcttctg tagaggttgc cgtatctagc atttttggta
    28561 aaattgatgc attgattgct gctgtccgag ttcttcttgt caatccatat aattttgtga
    28621 tgtcagaagc catcgttgta gggatcgatt ttaccgatgc tgcttcataa acaactcgtt
    28681 ctggttcccc attatctact ccaaatgcca tagaagaaac caagacctcc gctctttctc
    28741 tttctaaaga agaaatcgtt tctgaaacac ggtctacatt taagtgtttg acaggtaaga
    28801 atgcattgac aatctctatt tttttcttat tccctaaatc gatatcgttc aatctagttt
    28861 gtcgttttgc taaaaaacct tctaatccac cttttgccat cccgattacg ccgacttttg
    28921 ctacgtctcc ttcaatcagt gcattagtcg cttgtgtggt agaatgtgca acaaacacaa
    28981 catcttctgg actgatatta ttttcccgca aacaattttg aaaagactga acgacacctg
    29041 cggctactcc acggacatca tcgtgggttg ttttcactga agattttccg atgatttcgt
    29101 gcgtcgcatt atcgattgcg actgctttcg tatgtgtacc gccaacatca attcccattc
    29161 gcacaaaacg tttcattttc ttttcctcca ttcatctaat cgtttttatc gacgtttcgt
    29221 ttactaggcg ttcccgtata ataaaaacgt gatcaggcat aagatcgctc cgatgatcca
    29281 accagttggg atggatttct tcatatattc tcgagaagtt acttttgtat aacctaatcc
    29341 ccaagcaacc caagattgtg taatatctaa atgctgagga gcaatcgttg tgattgcaaa
    29401 taacgggaac aaaaaggctg gcgacatcgt tggattaaca gctaacacga cagctaaaat
    29461 agctaagcct gaaccgacta gattcattgg accgcggaaa aatcctagtg gtgtcaatat
    29521 agcaaatacg attgccaaga ctaatggtga ttgcggtatc caatctccaa aaatagctga
    29581 aaaataagga gaagcatacg cagctgaatt attgaacatc gctaaggtca tcaagaaacc
    29641 aaccattggt gcaacatcga ctgcgccatc tgtaaataat ttcgcaaact gacgacagat
    29701 ttctttataa cctccgcgca attttccagt aaacagcaat gcccagatac ctgctagaat
    29761 aaaacctaag atgattggaa gatctaaaag aacgacccct aaaactggta ataatacggc
    29821 taaccatgag atcattggtg cgtcgccacc ttcagctggt acgtttgctg ccattgcata
    29881 gcgtttcttt ttgttcattg atatattagc gaccgttagt accacaacta agcttacaat
    29941 catcccaatc ataccaattt ggaaatagtc attataagta tagctttcag ccgctggatt
    30001 gaaaccagca taaatcgttt gatattgttt gaaattgacg atattagcaa agattccagc
    30061 cataatcgag cccataaatg agaacagtgc aaccggtact gaaataccta atgacatcaa
    30121 gatcggcaac acaataactg caatagaaat cactggtccg attcctgtca tcgacatgaa
    30181 tataacagcg gttacaatac atagtaagct catcgtgatt cttggcttgt cccctccaag
    30241 ttcaactacc tttcgaatca gggtagctgc gattccgcta tctactagaa cacgcccgaa
    30301 aaaggcacca aagaagacat ttaccaagat cgatttggcg tactcagctg gccccgtcgt
    30361 atagatatgc gtcaacacat caattactcc ttgcccttcc atcgccgggt ttggtgaaaa
    30421 cgtgtttccg actagcgcta atcccatcca tacagtagcc atgatggcaa agccgaccat
    30481 caagttgtag ccttttacac agtaccagac catgaaaaag aatgagataa ctaacaaaat
    30541 accaataata atatcactca tgttattcct ccgtccattt tgtttttctg ataaacaaac
    30601 taatatatca gttaaactca ttctaactta tgcagttagc gctttcgtta aaggattacc
    30661 ataaatgttc ggatttcaat taaaaaacct taattatgct attttagact tatacgttat
    30721 tgtcctgttt catttgatgg aggattgtaa ataaaaaaag aggaaagaac ataacttttc
    30781 gttatcttct ttcctcttta taattgttta gcttatttag ccaaagcgtc ttttgcttgg
    30841 tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca agtcagctaa cattttacgg
    30901 ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt ttgagtagct caagccattc
    30961 atacgagctg ctgcgttgat acgagcaatc cacaatttac ggaagtcgcg tttcttttga
    31021 cgacgatcgc gatatgcgta gttgtatgaa ttcattactt gttcttttgc tgttttaaat
    31081 aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta gcactctttt acgacgttta
    31141 cgagttactg ttccaccttt aacacgtgcc atgtataatt cctcctaata aaaataaatc
    31201 ttcagattct gttatttgta cttcttattt catttttgct agttgttggc gaatacgttt
    31261 gaagtcgcca ctagaaacca tgcttgcttt acgcaattga cggcgttgtt tttttgtttt
    31321 gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc aatccgcctt taccagtacg
    31381 tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt ggcatgacta tatttcctcc
    31441 tcaaaatctt tcgttatccg actatcggct gtccaacagc cagttagtca ttcaaattac
    31501 ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc gtccgtccat tttcgctttt
    31561 tgttccactg ttgcaatatc cgcagtttct tcagctaagc gattcaagac tttttgacca
    31621 atttctttat gggtaatggc acggcctttg aaacggatag aagcttttac tttatcgccc
    31681 ttttccaaga acttacgtgc attacgaagt tttgtattga agtcattcaa gtcgatcgta
    31741 ggacttaagc gaacttcttt tacattgatc actttttgtt ttttacgcgc ttcgcggtct
    31801 ttcttttgtt gttcgaaacg gtatttaccg tagtccatga ttcgcgcaac tggtggtttc
    31861 gcaccagggg caactaacac aagatccaag tttgcttgtt ctgcaatatt caatgcttct
    31921 gcttttgtct taactcctaa ttgctcacca tctgaaccga tcaatcgtaa ctctcgtgca
    31981 cgaatgccgt cgttaaccat catatccttt gctatggtca ttcacctcca aatattatga
    32041 gagaaagaaa agcttaccga ataaacggca ataaaaaaac gagttctctt acagaacccg
    32101 cgcgtacttc acctaataga accagtgaaa actatctagc ccagtgacat tatcaactaa
    32161 ggcgagaagc gggtgcttct gcttttattt ctcaaccatc atagtatagc aaaaactaat
    32221 ctttttgtca atgtttttca tacagtttgt cttccttttc tgaaaatgat tagattagca
    32281 gactttctag cagtgattcc cgcttctttg ctatactaaa gaaaaacgag gtgaacccac
    32341 atgaaaaaaa tcgaacctat tccaccttct cttcctcttc ttacaaaggg agagttttat
    32401 ttagaagatg aggagatctt ctctggtctt ctgatcaacg ggggtgacca aagctatctc
    32461 gatgccaaaa atatggtatt aagagagagt catatccaaa aacttacgat gcaaaaaaca
    32521 gttttacgtc attttgaatg cagtaatgtc atttttgaaa aatgtgattt ttccaatctg
    32581 gagtggattg gtggcagttt ccaccaagtc gtgtttcatc aatgtaaatt aacaggtacg
    32641 aattttgccg aaagttatct tcgagactgt actttcactg attgtattgc ttcgatggct
    32701 tcctttagta gtacaaactt gaaagctgtc tccttcgatc attgtcaatt agaagacagc
    32761 gaattttatg aggtcacttg gaaaaacctt tttttgtctg acaatcagtt aaccggttcg
    32821 aactggtttc gaacttctct gaagggatta gactttacga ctaatttctt cacagcaatc
    32881 gctttatctc aagattcttt ggcgggacta atcgtcaacc aagagcaagc acttgtgatc
    32941 gcccaagccc ttggtttgca gatcaaagac tagatcaacg aatccgccgc cacagtgcaa
    33001 taagcatcca aatagataag accaaactga tgatcaaggc gattccccat ccgaaaacgg
    33061 aatgggtaaa gggtaactgg agattcattc cgaagaatcc tgtcacaata gttggaacgg
    33121 tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc attcaagttg ttattcagca
    33181 aattattata agtgccagag agctggtcta aaatatgcga agctaagttc gtcatttcaa
    33241 ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc tttttccttt tctgacaact
    33301 tgcgataaat agccaatgcc ttgatttgtt ctaaaagtac tacattttgt tttgttcctg
    33361 taactaagta aaccaaaccg atctctaaat ctgaaagttg cagcagattc ttgttcgttg
    33421 ttttttctct taatttcaga ttaagccgct gacgttcact attcacttcc tcgattaaag
    33481 gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa taaactgtat acactcatgt
    33541 ctggcattcg ttccaaaagc gatcgaatta gtcgaatcac gtactcggta ttcggcgtgg
    33601 taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat aggactagtt tcataatgat
    33661 tatcgacttt ttgctgatgc ggaacatttg atacaagcag taacgcttca tcaaaagaat
    33721 cgtactctac gcgcgcccgt tcatttttat ctagagaata agtcagcatc tcgtcactaa
    33781 tgccatattc ttcttttaag tttttaacag ctagcacatc gttttgctgt acattgatcc
    33841 aaaaattacg gtcttgatta aaatagtgtt tttcccgcat actctatcct cctgacattt
    33901 gtttccagta taaagaaaaa agtcgctcaa ataaaggttt accctttatt tgagcaactg
    33961 taaatcaaat ggctactttg gtttggcgaa tctctgctaa tttttcttcg atcattttca
    34021 agacttcttc ttttgcttga ggatcttcca caaaattata gcggtctcca tcgatttgga
    34081 tttttggact ttctttgtat tcttcatacc attgatcgta acgtctgttc aattctttgt
    34141 aataatcgta tagacttgga tcaaaggaca attgttcata cgaacgacca cgtttttcga
    34201 tacgctccaa catcgtatca aaagatacac gaatatgaac taataaatcc ggatgttttt
    34261 tatgggcagc atagggaagt tcttccatca tgttttccag cagttcatca tacacacgaa
    34321 cttctgtttc tgttgctctt cctagatctg catttagatg gaaaagcagt gaatcttcat
    34381 aaatcgaacg gtccaataca ttattgtcat ctttcattgc ttgtttgatg cttgcaaaac
    34441 gtttattcaa aaaataaatt tgcagcaaaa aagcatactg ttcagggttt gcataaaata
    34501 agggcaagac ttcattatca tcaatcgatt cataaaatgc ctgactatca aagtgttcag
    34561 ccatcatttc tgttaaactt gattttccgg caccaattgt acctgctaaa acgatcacgc
    34621 tcatttacct ccagctcact tcatcactaa atattgtgtt taactccaac ctttttaaat
    34681 atacccccaa tatctagtag tgtcaattgt atttttttct agtcaaagaa gaaaaaaagt
    34741 caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa cgtattctta gttttacttc
    34801 tgagatggtt ttagtaaaat aaaggaggaa tgttttttga gacccttagt ggacaaaaaa
    34861 attcgtgaag cagcgcaact cgttttgggc gctttatttt ttgcttaaaa atcactttta
    34921 cgtgagaagg agttagatta tggcaaacta tggccaagga gaatcaagcg gaaagatcat
    34981 attgatgggc gagcacgcgg ttgtttatgg agaaccagcg attgcctttc ctttctatgc
    35041 aacaaaagtc accgcattcc ttgaagagct ggatgcaatg gacgatcaac tggtttcttc
    35101 ctactattca ggaaatttag ccgaagctcc tcatgcatta aaaaatatca aaaaattatt
    35161 cattcactta aaaaaacagc atgacatcca aaaaaacttg caactgacca ttgaaagcac
    35221 gattcctgct gaacgtggaa tgggatcaag cgctgcagtc gccacagcag tcactcgtgc
    35281 tttttatgat tacttagcat ttcctttgtc tcgtgaaata ctattagaaa atgtccagct
    35341 ttcggaaaaa attgcccacg gtaatcctag tggaatcgat gcagccgcta ctagcagctt
    35401 gcagccgatt tactttacaa aagggcatcc tttcgactac ttttctttga acatcgatgc
    35461 ttttttgatt gtcgctgata caggaatcaa aggacaaaca agagaagccg tcaaagatgt
    35521 cgctcacctc tttgaaaccc agcctcatga aactggacaa atgatccaaa aattaggata
    35581 cttgacgaag caagcaaaac aagcaatcat tgaaaattca ccagaaacgt tggcacagac
    35641 aatggatgaa tcacaatcac ttctggaaaa gctgacaatc agcaatgatt ttcttaatct
    35701 attgatccaa acagcaaaag ataccggggc attgggtgcc aaattaactg gcggtggacg
    35761 cggtggctgt atgattgcat tagcacaaac aaaaacaaaa gcccaagaaa tcagtcaagc
    35821 acttgaagat gcgggtgctg ctgaaacttg gatacaagga ttaggagtac atacctatgt
    35881 ttaaaggcaa agcacgcgca tataccaaca ttgctctaat caaatattgg ggtaagaaaa
    35941 atgaagaact catccttcca atgaacaaca gcctttcgtt aacattggat gcattttata
    36001 cagagacaga agtcatcttt tctgatagct atatggtaga tgaattttat ctagatggca
    36061 ccttgcaaga cgaaaaagca acaaaaaaag tcagtcagtt tcttgacctt tttcgtaaag
    36121 aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa tttcgttcct accgcagctg
    36181 gattagcctc ctctgccagc gggctagctg ctttagcagg agcttgcaat actgctctta
    36241 agcttggatt agacgatctc tctctttcaa gatttgctcg acgcgggtct ggttcagctt
    36301 gccgaagtat tttcggtggt ttcgtcgaat gggaaaaagg ccatgacgac ttaagttctt
    36361 acgctaagcc agtcccttcc gattctttcg aagacgattt agcaatggtt ttcgttttga
    36421 tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat gcgtcggaca gtcgaaacat
    36481 ccagttttta tcaaggctgg ttagattccg ttgaagggga tctatatcaa ttgaaacaag
    36541 caatcaaaac aaaagatttc caacttctcg gagaaacgat ggaaagaaac ggactaaaaa
    36601 tgcatggaac gactttagct gcccagcctc cattcactta ctggtctcct gacagcttaa
    36661 aagcgatgga tgcggtgcga caattgagaa agcaaggcat tccatgctac tttacgatgg
    36721 atgctggccc taatgtcaaa gttttagtgg aaaacagcca tttatctgag gtacaagaaa
    36781 catttaccaa attatttagc aaagaacaag taattactgc ccatgctggt ccaggaattg
    36841 cgattattga ataaagggag atttttatga ttgaagtatc tgcaccaggc aaactctata
    36901 ttgccggaga atatgcagtt gttgaaacag gccatccagc agttatcgct gcagtcgatc
    36961 aattcgtgac agtcactgta gaatctgcac gaaaagtcgg aagtatccaa tctgcacaat
    37021 atagtgggat gcctgtacgt tggacaagac gcaacggaga attagtttta gatattcggg
    37081 aaaatccttt tcattatatc cttgctgcta ttcgcttgac tgaaaagtat gcacaagaaa
    37141 aaaacattct tttatccttt tatgatctga aagtaacaag tgagttagac agttcaaatg
    37201 gccggaaata tggattgggt tctagcggtg ccgtgaccgt tgcaacggtc aaagccttga
    37261 atgtttttta cgcgttgaat ttatctcagt tggagatttt caagattgca gcactagcca
    37321 atttagcagt tcaagataat ggttcctgcg gcgacatcgc tgctagctgt tatggtggct
    37381 ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga acaagaaact cagcattcta
    37441 tcagtgagtt acttgccctg gattggccag gtctatccat tgagccattg attgctcctg
    37501 aagatttacg tttattgatt ggttggacgg gtagccctgc ctctacttct gatttggtcg
    37561 atcaagttca ccgttcgaga gaagataaaa tggtggctta tcagcttttc ttaaaaaaca
    37621 gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga aaataatgta acgttgatcc
    37681 aacagatgat tcgaaaaaac cgacaattac tgcatgattt atctgcaatc actggggtcg
    37741 tcatcgaaac gcctgctttg aacaaattgt gtaatttagc tgaacagtat gaaggagccg
    37801 caaaatcttc tggtgcaggt gggggcgatt gcggaatcgt aattgttgac cagaaatctg
    37861 gcattcttcc tttaatgagt gcatgggaaa aagcagaaat cactccactg ccgttacatg
    37921 tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa gacgaacatg tttcactagc
    37981 taaagccttt catgataaac aaaaaaacga atttgacttc gtacggatca tccataaccc
    38041 tttgccgcaa atagcagttt ctgatgtcga tctaagtaca caggcagttg ggtttacact
    38101 aagcagccca ttttatatca atgcgatgac tggcggaagc gaaaaaacaa aaaaaatcaa
    38161 ccaagatctt gcaatcgtcg caagagaagc agatttgatg attgccactg gttcagtcag
    38221 cgctgccttg aaagaccctt ctttagccga tacttatacg attatgcgac aagaatatcc
    38281 tcacgggaaa atcattgcca acatcggtgc agggacttcc gtcgaaagag cccaagaagc
    38341 tatccgattg tttcacgcag atgccttaca gattcatttg aatgcacctc aagagttggt
    38401 tatgcctgag ggagaccgtg attttaccaa ttggaaagta cttattcaag agactcaaac
    38461 agccattgac gtccccctca tcgtaaaaga agtcggtttt ggcatgacaa gagaaacact
    38521 caatgatcta gcttctttag gagttcatac agttgatatc agcggtcgaa gcggaacaag
    38581 cttcacccag atcgaaaacg cccgtcgctc caagcgggaa ctgagttatt tagctgactg
    38641 ggggcaatcg acagtctctt cattgcttga agcaaatgaa gcagacactt ctatggaaat
    38701 cctagcctcc ggaggtatcc gcaatgccta tgacatattt aaagcccttt gtctcggcgc
    38761 aaatgcagtg ggtacttcag gaaccgtact gacgcatctg atgaatcatg gcgtagaaga
    38821 aactattata ctgatgaagc aatggcaaga agagcttcgt ctgctttata ctatggtcgg
    38881 cgcaacgaat accgcggccc tacaccaaca atctctgatc ttttccggac cagtgaagga
    38941 ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga agacgcacag aaaagagcgt
    39001 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtacaaac ggcggaagca
    39061 aaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
    39121 attccttctt atttcttgga gttaaacgct tctgtcccag cctctttttt cgaataaatg
    39181 atgtttaatc agctgtcctt cggtattcgt tcaatccgct gatttcacaa cctctccact
    39241 aaacagtgta ctaaagctct tttaaggttg ttttccgata taggcaagta tgcctccgtc
    39301 cacatacaag acatggccgt taacgaaatc agatgcgcta gaagccaaga agacagatgg
    39361 tccagccaaa tcgattggct cgccccagcg tgctgctggc gtacgtccaa cgataaattg
    39421 atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt
    39481 agcaatataa cctggtccaa ttccattaca ttggatattg tattgaccgt attcagaagc
    39541 gatgtttttc gtcagcattt ttaatccgcc ttttgccgca gcataagcac tgaccgtttc
    39601 gcgtcctaat tcactcatca tggagcagat attgatgatt ttaccgccgc ctttttcgat
    39661 catatccgga ataactgctt ttgaaacgat gaatggggca tttaaatcca cgtcgattac
    39721 ttggcggaag tctgccgcat ccatttcgat catcggtgtc cgtttgatga tccctgcatt
    39781 gttgactaga atatcaactg aacctacatc ttctttgatc tgttgaacca tcgcttgaac
    39841 cgcttcttca tcggtcacat cgcagacata accttttgca tcgatgcctg cttctttata
    39901 atgcgctata ccttcatcaa ctgattcttg attcaagtta ttaaatacga ttgttgctcc
    39961 tgctgtagct aaagagcgtg caatctcaaa tccaatacca tagacagcac cggtgactaa
    40021 tgctactttt ccatctaaac gaaacatatc catactaaat tccatcattt tctctccctt
    40081 atgcttattt taattgatcc atttgcacca tatccatgtc tgtataggtg atattttctc
    40141 cgcacattgc ccagatgaat gagtaattgc ttgtacctac acctgaatgg atcgaccagc
    40201 ttggcgaaat aatggcttgt tcattgtcta caactaaatg ttttgtttca tctggtttgc
    40261 ccatcatgtg gaacacacgt gtatcttcat tggcataatc aaagtagaca taagcttcca
    40321 tacgacgttc atgtgtatga caaggcatcg tattccaagc acttcctggt tctaaaatcg
    40381 tataacccat ttgtaattga cagctttcgc atacatttgg atggatgtat tgatagattt
    40441 tacgttcatt cagtgtcagg ccttcaccag tttccattgg ttttacttgg tcgatactga
    40501 tttttacatt tggatattta tgatgagctg gaacagaact aatatagaat ttagctgggt
    40561 tttcagcatc ttgagaagaa aatacaacat gtcgtgtttc ttttccgata tagtatccgt
    40621 cttgtttttt catttcttct ttttgcccat cgatctcgat gaatcctggt ccaccgatat
    40681 tgatgacaat caatagaagg ctaggaatcg cctcttttat tatttttcca gcatgagcca
    40741 tctgtccttt ttcgacaaca gggtatttat tctttttagc aacgatgaat gctactagca
    40801 tgattcctag tgcccacaaa gcccctacga gataacctcc cataaataaa gccgaaatcg
    40861 aagcaccacc agcaacaagt gaatacatga taaaagcagt acttggagga atgaccatac
    40921 ctgtcggagc tgacgcgata ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca
    40981 cttgctgagg gatcaagact ccgccaatcg cggtagaagc ggcaatggct gagctagaga
    41041 tggaaccaaa taaagcattc cctaacacat tcgtatgagc caaagccccc ggtacgcgtc
    41101 cgacaaacaa catcgcaaaa tccactagtt tcttggctat ccctccatta ttcatgataa
    41161 tacctgaaag aacgaaaaac ggaatagcga tgagcgaaaa actgttgata cttcccacca
    41221 ttttttgagc tgaactaaaa gcagaaacat caaacggtat aactaacaat aaagtcatca
    41281 ttgatgagaa cgcaatactt atagcaatcg gcatccctaa tacaagcaac acgacaaata
    41341 caaaaaataa cactaatccg gcttctattg ccattttatt cctccatttt tatatttttt
    41401 cttgaattgc tggcttgttg cttaatgtat cgattagatt gatcagacta tagataatga
    41461 tgagtatacc tgagacaggt attgacaaat agacgtaccc catcggaatg cttaaagaag
    41521 gcgagatctg tcccattgtc aatgtgacag cattccatcc gccatacacc atgatcatcg
    41581 cagcaaatac cagaaataaa ctttgaacaa cgacttctaa aatcttactt tttttaccag
    41641 ataatttttc acttaaaacc gttacggcta aatgtgcttt ttgtcccaca acatacgcag
    41701 aagacaacat ggcaaaccag acaagtccga atcggacaag ctcttctgta accgtattcg
    41761 gatttcctaa aaccgttctg gaaaatactt gccataaaac gacagcgacc atcccaacaa
    41821 gcaaaacagc tcctaggatc tctaacacct tatttaaaat cattttcatc acagctacgc
    41881 tccttgctct tggatctttt gataaaggtc tttgtaggca ggatcgtttt tgaatgtatc
    41941 atgaagcggt gcgactgcct ctcgaaacgg tgtattatct acatcattga attggactcc
    42001 atgttcttct tgtgctaaag ctttttcttc ttcaattgct tttgcaaaag cttcaatttc
    42061 aaaaaccgtt gattctttcg ctgcttcttc aatggctttt tcctgctcat cagttagctg
    42121 ttctttgatg gcatcattca tgatgatgat atctggcaca cgcgtatgct catcatagga
    42181 atagtattta gccacaccac catgtccagc tgtatacaag acaaattcat tgttttctga
    42241 tccgttgatc aaattggatt gaagagacgt gtacacttca tcacttccca taggtacggg
    42301 cgaaccgcct aaaagctcta ccattttgat agcggtttcg ctttgcatca cacggatctt
    42361 ctttcccttc aaatcttccg gcgtatggat cggaccgtct accatataaa agctgcgctg
    42421 accagaatca taataggtca atccggtaaa tcctaaattt ttggtcgatt ggtagattgg
    42481 ctccattatt tcttcattat ccatcacctt aaagaaatgt tcttcatcat tgaatagata
    42541 aggaacggaa aaaatcgaat aatctttaga gaaactttcc aacgctgagg cacttacttt
    42601 tgtgaaatct actgctcctg actgtgtcaa ttcgatcagt tcacgttctc cacccagttg
    42661 tccatctggg aaatactgga tctgtacgga accatctgtt ttttctttta ctaaacgccc
    42721 aaattcattc attgcatcaa ttactggctg gctgttgctt gcataagcaa agcgcaaggt
    42781 aactatctca tttacgtctg catctgcgac agttgccaca ctagaattat ttccgcagcc
    42841 gcttagacct agcagaccta ggctaagcga acttgctgca aacagcagtg atgttttttt
    42901 catatgggtt tcctttcttt taacaataag agacgagcaa ttctgtcaaa caaagcaccg
    42961 ttaacgactg tccataaggc attgctgtga ttccgatatt ccgataaaaa tctaaactgt
    43021 cccccattcc tgtcccgatt gaaacgtttt gaacttctcc tttttcatcg atttgttcaa
    43081 gcaatccttg gatagcacga taagcgactt ctttatattc ttgtggtaaa taacgtttat
    43141 ggaccgcttt caaaataccg taagcaaatc ctgcagttgc agaagattct acgtaagaag
    43201 aaggatcatc caacaacgta tgccataaac cgctttcaga ctgataagtt tttaatgcac
    43261 gcacttgtgc ttcaagtgtt tcgatcaata atttgcgcaa tgcatcttct ttcgataatt
    43321 ctaagatttc aatgatctct ggaatagcaa tcgtcaacca gcagtttcca cgagcccaaa
    43381 atgcttcggc atagttatga tttccttcaa acgtccagcc gtgataccac aatcctgttc
    43441 gtttatccat caagtatttg atatggatca taaactggta acgagcttct tctaaatatt
    43501 ctgggcgatt caacagtttc ccgatttttg ccaaaggaag aactgtcatc atcaatgtat
    43561 cgtcccacaa ctgattctta ttttccggtc catatgtggc atgctgtaac ccgtcttcat
    43621 ttgtccgagg catgtcattc atgacccatt ctgcccattg ttctaaataa ggcaaatagc
    43681 gtgaatcttt cgtgtcttca tacaagtaag ccatagtcaa tagcggtgcc attgtattga
    43741 cgtttttggg cggagcgcct tcttccatac gatcctcaaa ccattcattg accacttgat
    43801 aagcgcgagg attttttgtc atctggtaat ttttataaat accatataga ccgacacctt
    43861 gtggccaatt ccacactgtc cagctcttgt catctacttt cagtccatca aagtccaaca
    43921 aaaattcacc attatcgtct ttgatcgttg tcaggttttc aacgagcagg tcaatttttt
    43981 ctttcactaa ttctttaggt acaaaaatct cttctttaat aatcatcttt acgccacctt
    44041 tttaaacgct ttcattttat atccttaata tagcagtaat accgtctact ataaatatcg
    44101 ctttttgaac aaaaactgtc taaaattgac ctaaatgtta aataacaaaa gtaactttca
    44161 gttacttgga aattaaaaaa agaacttgga gaaacaacgt tttccaagtt ctttttcatc
    44221 tattttctta tcttttttct acgctttctt ttgcttatcc gttgtaagca tcaatgatga
    44281 tctgtttcaa ttcagagatc aatggttctt tcggattagc tgtcgtacat tggtcttcgt
    44341 aagctaattc agccatacgg tctacggttg tatctagtgt ttcttgtgtg actccttgag
    44401 cttttagact catgttgatg ccgacagatt ttcctaattc ataaacagcc gttgctaaag
    44461 cttcgactaa ttcctctgtt gtttcgcctt tcagacctaa gaattttgca atatctgcgt
    44521 aatctgtatc tgcacggaaa tagtcatatt tagggaacat cgcatgtttt tgtggatctt
    44581 ttgcattata gcggataata tgcggtaaaa gaatcgcatt tgtccgtcca tgagggatcc
    44641 cgtattcccc gccgatttta tgtgcgactg agtgacaaat acctaggaat gcgttagcaa
    44701 acgccatacc agccattgta gaagcgttgt gcattttttc acgtgattcc atatcaggtg
    44761 tttttactga tttttctaaa tagtcaaaga ctaacttgat tgcttgtagg ctcaatccac
    44821 gtgtataatc tgaagccatg actgaaacat aggattcgat tgcatgtgtt aacacatcca
    44881 tcccggtatc tgctgtgaca gatgctggaa ctgacatgac gaattgagga tcgacgattg
    44941 cgacatctgg cgtcaatgca taatctgcta acggatattt cacatgtgtt tcactgtcag
    45001 tgatgaccgc aaatggcgtt acttcagaac cagtccctga tgttgtcggg atacatacga
    45061 attgtgtttt ttctggtttg tcgatcttat atgtccgttt acggatatcc aagaattttt
    45121 gttttgcacc aaagaaagat gtgtctgggt gttcatagaa catccacatt cccttagctg
    45181 cgtccattgc agaaccgcct ccaagagcga ttactgtatc tggcttgaag tctgccatca
    45241 gtttcgttcc tgcgtatacg gtatttgtcg atgggttcgg ttcaacatct gagaagactt
    45301 cgatcttcac atcattttta cgtcgtgcca atacttcacg aactgtatcg cagtaaccga
    45361 attggaccat accagggtca caaatgatca tgacacgttc aacgttttcc attttctcta
    45421 agtacaatag agagttcttt tcaaagaaga tttttggcgg taatttgaac cattgcatgt
    45481 tatttctccg tttcgctaca gttttgacat tgattaagtt tacagcagag acgttacgag
    45541 aaacagagtt cttaccataa gaaccgcatc ccaaagtcaa tgacggaatc atttcgttat
    45601 agatatcacc gatgccgcct tctgcagatg gtgtattcac taaaatacga caagctttca
    45661 ttcgaagacc gaatttgaca tgcaactctt cgttttctga atggataact gctgtatgtc
    45721 ctaatcctcc aagattcaac ataccttcac acaaatcaaa cgcatggtcc gtgttgttag
    45781 ctttgatcat ggcaagaact ggagaaagtt tttctcttga tagcggataa tctgggccta
    45841 cgccttccaa ttcagcaatc agcattttcg ttccttttgg tacactgatt cctgccaatt
    45901 ttgcgatttc catcgcagaa tgtccaacga ttgatggatt gaccgcatat tttccttcgt
    45961 tcatcactgc atcttctaat ttttgcaatt catctggttt tacgatataa acttggtgtg
    46021 cttggaattc tgcttttact gctgcataga tttctttatc cacgatcaca gcttgttcag
    46081 aagcacaaat cattccatta tcaaatgttt tcgaaacgat caaatcgttc actgcacgtt
    46141 taatttttgc tgttttttca atataagctg gtacgttacc tggacctaca cctaaagctg
    46201 gttttcctgt agagtaagca gacttgacca ttccagctcc acctgtcgct aagacgatcg
    46261 cgatacctgg atggttcatt agcatagaag tcgcttcgat cgaaggatgt tcgatccatt
    46321 ggatacaatt ttcaggagct ccggcagcga tagcagcatc acgtactact cgtgctgctt
    46381 ctgcagaaga tttttgggca cttggatgga aagcaaaaac gatcggattt cttgttttca
    46441 acgcgatcat tgatttgaaa atcgttgtgg aagtcggatt cgttgttgga gttactccgc
    46501 aaaccacacc gactggttca gcaatttcaa tcaatccttt ttggacatct tcattgatca
    46561 caccaactgt tttatcatgt ttgatattat gccaaatata ttcggacgca tacatatttt
    46621 tgattgcttt atcttcgtaa acaccgcggc ctgtttcttc cacagccatc ttcgctaatg
    46681 gcatatgttg atctaaagct gccattgcca tttcatgcac gatatggtcc actttttctt
    46741 gatcaaagct ttccatttct ttcaatgctg catttgcttt ttctgccaaa tcatcaatca
    46801 ttgcttgtac gtcaattgtc ttcgtttttt ctttttccat tactttagcc atcattatcc
    46861 tcctaatggg tcatggttat ttgttaaatc attcacatgt atatcataca atatttgttt
    46921 ttctatgtaa acactttttt gtgatttttt tcacagttat ttttaaatat aaaattgctt
    46981 tatttacagg tgtttgtata gaaaaatata taaagttatt tattattatt tatatg
//
LOCUS       DAJSVF010000015        44710 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSVF010000015 DAJSVF010000000
VERSION     DAJSVF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.015"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            200..2734
                     /gene="secA"
                     /locus_tag="ON550_000811"
     CDS             200..2734
                     /gene="secA"
                     /locus_tag="ON550_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR3953916.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2860..3961
                     /gene="prfB"
                     /locus_tag="ON550_000812"
     CDS             join(2860..2931,2933..3961)
                     /gene="prfB"
                     /locus_tag="ON550_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR3953917.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4066..4752
                     /gene="ftsE"
                     /locus_tag="ON550_000813"
     CDS             4066..4752
                     /gene="ftsE"
                     /locus_tag="ON550_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR3953918.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4736..5629
                     /locus_tag="ON550_000814"
     CDS             4736..5629
                     /locus_tag="ON550_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953919.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5781..7229
                     /gene="cls"
                     /locus_tag="ON550_000815"
     CDS             5781..7229
                     /gene="cls"
                     /locus_tag="ON550_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3953920.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7566..8423
                     /locus_tag="ON550_000816"
     CDS             7566..8423
                     /locus_tag="ON550_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR3953921.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8538..9458
                     /gene="pstC"
                     /locus_tag="ON550_000817"
     CDS             8538..9458
                     /gene="pstC"
                     /locus_tag="ON550_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR3953922.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9458..10342
                     /gene="pstA"
                     /locus_tag="ON550_000818"
     CDS             9458..10342
                     /gene="pstA"
                     /locus_tag="ON550_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR3953923.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10357..11163
                     /gene="pstB"
                     /locus_tag="ON550_000819"
     CDS             10357..11163
                     /gene="pstB"
                     /locus_tag="ON550_000819"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3953924.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11179..11937
                     /locus_tag="ON550_000820"
     CDS             11179..11937
                     /locus_tag="ON550_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3953925.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11950..12627
                     /gene="phoU"
                     /locus_tag="ON550_000821"
     CDS             11950..12627
                     /gene="phoU"
                     /locus_tag="ON550_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR3953926.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12865..13896
                     /locus_tag="ON550_000822"
     CDS             12865..13896
                     /locus_tag="ON550_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3953927.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13893..14660
                     /locus_tag="ON550_000823"
     CDS             13893..14660
                     /locus_tag="ON550_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3953928.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14760..16328
                     /gene="liaX"
                     /locus_tag="ON550_000824"
     CDS             14760..16328
                     /gene="liaX"
                     /locus_tag="ON550_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR3953929.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16625..16945
                     /locus_tag="ON550_000825"
     CDS             16625..16945
                     /locus_tag="ON550_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR3953930.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16945..17295
                     /locus_tag="ON550_000826"
     CDS             16945..17295
                     /locus_tag="ON550_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3953931.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17462..18400
                     /locus_tag="ON550_000827"
     CDS             17462..18400
                     /locus_tag="ON550_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR3953932.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18415..19245
                     /locus_tag="ON550_000828"
     CDS             18415..19245
                     /locus_tag="ON550_000828"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR3953933.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19289..20314
                     /locus_tag="ON550_000829"
     CDS             19289..20314
                     /locus_tag="ON550_000829"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3953934.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20329..21267
                     /gene="galU"
                     /locus_tag="ON550_000830"
     CDS             20329..21267
                     /gene="galU"
                     /locus_tag="ON550_000830"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3953935.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21392..21832
                     /locus_tag="ON550_000831"
     CDS             21392..21832
                     /locus_tag="ON550_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR3953936.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21835..22437
                     /locus_tag="ON550_000832"
     CDS             21835..22437
                     /locus_tag="ON550_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3953937.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22496..23602)
                     /locus_tag="ON550_000833"
     CDS             complement(22496..23602)
                     /locus_tag="ON550_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3953938.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23812..24813
                     /gene="ccpA"
                     /locus_tag="ON550_000834"
     CDS             23812..24813
                     /gene="ccpA"
                     /locus_tag="ON550_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR3953939.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25150..27495)
                     /locus_tag="ON550_000835"
     CDS             complement(25150..27495)
                     /locus_tag="ON550_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3953940.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27774..28676
                     /locus_tag="ON550_000836"
     CDS             27774..28676
                     /locus_tag="ON550_000836"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR3953941.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28889..30664
                     /locus_tag="ON550_000837"
     CDS             28889..30664
                     /locus_tag="ON550_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR3953942.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30730..32316)
                     /locus_tag="ON550_000838"
     CDS             complement(30730..32316)
                     /locus_tag="ON550_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR3953943.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ON550_000839"
     CDS             32566..32790
                     /locus_tag="ON550_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR3953944.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ON550_000840"
     CDS             32932..34683
                     /locus_tag="ON550_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3953945.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ON550_000841"
     CDS             34683..36467
                     /locus_tag="ON550_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953946.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="ON550_000842"
     CDS             36793..37644
                     /locus_tag="ON550_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3953947.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="ON550_000843"
     CDS             37698..39449
                     /locus_tag="ON550_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3953948.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="ON550_000844"
     CDS             39453..39623
                     /locus_tag="ON550_000844"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3953949.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="ON550_000845"
     CDS             complement(39663..40565)
                     /locus_tag="ON550_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3953950.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="ON550_000846"
     CDS             40796..42199
                     /locus_tag="ON550_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3953951.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="ON550_000847"
     CDS             42199..43632
                     /locus_tag="ON550_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3953952.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="ON550_000848"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="ON550_000848"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR3953953.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
BASE COUNT        14411 a         7850 c         9618 g        12831 t
ORIGIN      
        1 gaaaacttgc agcagctact gacagctgcc gcaagttttt ttgagtttcc tcctctttta
       61 gatgaaatat gaataaatta tgaagttctt actttaatac gcttaagata tttcaattat
      121 ctataactaa tgataaaatg aaaagtaaag tctacatagt ctggataata cacagaagga
      181 aaaggagatt acacaacgca tggccaattt gattcgttca cttatcgaaa acgataagaa
      241 agaactaaaa cgtttagata gcattgcaaa aaaagttgaa gcatacgctg accaaatggc
      301 agcattaact gatgaacaat tacaggcaaa aactgctgaa ttcaagggca gataccaaaa
      361 aggagaaaca ttagatcaat tacttccgga agcttttgca gtcgtccgag aagcagcaaa
      421 acgtgtattg gggttatatc cacaccacgt acaattgatg ggtggaatcg tgcttcacga
      481 tggaaatatc cctgaaatgc gtactggtga aggtaaaacc ttgaccgcaa cgatgccagt
      541 atatctaaat gctctgactg gcgaaggggt acacgtagtt accgtcaatg aatatttagc
      601 aacccgtgac tcgacggaaa tgggcgaact ttataatttc ttaggattaa cagtggggtt
      661 gaatatcaac tcaaaatctt cggatgaaaa acgcgaggct tataattgtg atatcactta
      721 tagtacgaat aatgaattag gatttgacta cttgagagac aatatggtcg tttaccgcca
      781 tcaaatggta caacgtccgc tgaattatgc cattgtcgat gaagttgact caatcttgat
      841 tgatgaagcg agaacgccgc tgatcatttc agggcaagca gaaaaatcca cagctttata
      901 tacaagagca gacaacttcg tgaaacgttt gaaagaagaa gaagattata aaatcgatgt
      961 acaatcaaaa acaatcagtt taaccgaagc agggatcgaa aaagcagaag aaacattcgg
     1021 tttggataac ttatacgaca ttgaaaatac tgcattgacc catcacttgg atcaagcatt
     1081 gagagccaac tatatcatgc ttttagatat cgattatgtt gtccaagacg gaaaagtact
     1141 gattgttgac cagttcactg gtcggatcat ggatggacgc cgttattcag atggacttca
     1201 ccaagcaatc gaagctaaag aaggcgtaga gatcgaagac gaaacgaaaa ccatggcgac
     1261 gatcactttc cagaactatt tccgtatgta caagaaactt gcgggaatga ctggtacagc
     1321 taaaacagaa gaagaagaat tcagagaaat ttataacatc caagtatacc agattccgac
     1381 aaatcgtccg gttatccgtg atgatcgtcc agatttgctt tacccaacat tacaaagcaa
     1441 gttccacgct gtcgtgcaag atatcaaaga tcgctaccgc aaaggtcaac ctgtgttagt
     1501 cggaactgtt gcggttgaaa catctgagtt attatcagat atgctgaatc aagaacgtat
     1561 tcctcatgaa gtgctgaatg cgaaaaatca ctttaaagaa gcagagatca tcatgaatgc
     1621 tggtcaaaaa ggtgctgtga cgattgctac caacatggct ggtcggggaa cagatatcaa
     1681 gttaggactt ggggtaagag aacttggtgg tctagcagtt atcgggacag aacgacatga
     1741 atctcgtcgt atcgataacc agcttcgagg acgtgcagga cgtcaaggag accctggtat
     1801 gtcacagttc tacttgtcat tggaagatga tttgatgaaa cgattcggtt cagaacgaat
     1861 caaatctttc ttagatcgga tgaagatcgg tgaggaagat gctgttatcc aaagcaagat
     1921 gctgacgaaa caagtagaat ctgcacaaaa acgtgtagaa ggaaataact atgacacacg
     1981 taaaaacgtc ttacaatacg atgatgtgat gcgtgaacaa cgtgaagtaa tctataaaca
     2041 gcgacaagaa gtaatcatgg aagaaaaaag cttatccaag ccactaatga acatggtcaa
     2101 acggacaatc agtcgtgtag tagatgcgca tacacaatta gagaaagaaa attggaattt
     2161 ggaaagtatc atcgattttg ccggcagtac tcttgtccat gaagatagta ttactcttgc
     2221 agatatcgaa ggaaaaacag ctcaggagat caaagagtat ctaaacgaac gagcacaaga
     2281 agtatttaat accaaagcgg ctcaactaaa tggtccagag caactgttgg aatttgaaaa
     2341 agtcgtgatc ttgcgtgttg tggactctaa atggacagac catattgatg caatggatca
     2401 attacgtcag tcaatcggct tacgtgctta tggacagaac aatccattag tcgaatatca
     2461 aacagaaggc tataagatgt ttgaagatat ggtaggcgcc atcgagtatg aagtcactcg
     2521 aatctttatg aaagctgaga tccgtcaaaa cgtacagcgt gaacaggttt ctcaaggaac
     2581 agcttctcac tcagaagacg gagatgccaa agaaaatagt aatactagcg cgaaaaaaca
     2641 gccaatccat gtgaacaagg tcggaagaaa tgatccttgt ccttgtggaa gcgggaaaaa
     2701 atataagaac tgtcatggaa aaaatgcaaa ctaatttctg acagcaggat tggtgagaca
     2761 aattcttttt ttaagaattc gtctcacctt ctttctgtta tttgctgatt ctgttagact
     2821 agatataatg aaaaaagtat agtgaaagaa ggaaaaaaca tggaaatatc cgaaattcga
     2881 aatctcctga cagaaatgca ggaaaaggtc acaagcttca ggaggtctct ttgacttaga
     2941 ccagctggaa gaagacattg cggaagcaga agcaaaaatg gctgaacctg gtttttggga
     3001 tgatagcgaa gctgctcaaa aagtgatcag tgaaaacaat gcaaataaag aaacttatga
     3061 tcaattccat tctcttgctg aagaactgga agaaatcgaa ttgatggtgg agatgttgca
     3121 ggaagaacct gatgcagcca tgcaagaaga agcagaaaca cgtatcaatg cgctagatga
     3181 aaaaatgaag acatatgaat tatctatgtt gttagatggt ccttatgatc gaaacaacgc
     3241 catcgttgaa ttgcatccag gggctggcgg aacggagtcc caagattggg gcagtatgct
     3301 tttacgtatg tatacacgct gggcagagca gcatgggtat caagtagaga cactagatta
     3361 tcaagcaggt gatgaagcag gaatcaagag tgtgacccta ctgatcaaag gacacaacgc
     3421 atttggttat ctgaaatcag aaaaaggtgt tcatcgcctt gtacgtatct cgccttttga
     3481 ctcagcgaaa cgacgtcata cttctttttg ttcggtagat gtgatgccag agcttgatga
     3541 aacgatcgat atagaaatca atccagatga tttgaaaatc gatacgtacc gagcaagtgg
     3601 tgcaggtggt cagcacatca ataaaacaga atctgctgta cggatcactc acttgcctac
     3661 aggaacagta gtagctagtc aggcacaacg ttcacaattg aaaaaccgtg agcaagcgat
     3721 ggggatgtta aaagctaagc tttatcaatt agaagtggaa aagaaagaac aagaagcagc
     3781 tgcacttcga ggagaacaaa aagaaatcgg ctggggttca cagatccgtt cttatgtttt
     3841 ccatccatat tctatggtaa aagaccatcg gacaaacgta gagacaggaa atgtccaagc
     3901 agttatggac ggtgaccttg atttatttat tgacggctat ttaaaaatgc atctacaata
     3961 agaacccatt gttaaattga aataaaattg taagattttg caagcatgtt gaaacattgc
     4021 catgctataa tagctaaaga ctgaaaaaga tatggagaga acgccatgat tgaaatgaag
     4081 gatgtaatga agaagtactc aaatggtacg accgctatcc gcaatctctc aattgtcatc
     4141 gaacaaggag agtttgtcta tgtggttggt ccgtctggag ccggtaaatc tacattcatc
     4201 aaattgatgt atcgagaaga aaaagcttca aaaggaactt tgaacgtcgc aggtcatgat
     4261 ctaatagcaa tcaaaaaccg cgaagttcct tatctacgca gagaaatcgg cgtggtgttc
     4321 caagactata aattattacc aagaaaaaca gtatatgaaa atgttgcgta cgctatgcaa
     4381 gttatcggca gaaaacctcg ggatatcaaa aaacgagtaa tggaagtgct ggaccttgtt
     4441 ggtttgaaac acaaagttcg agtctttcct agcgaattat ccggtgggga acagcaacgt
     4501 gtctctattg ctcgggcaat agtgaatacg cctaaagtac ttatcgcgga tgaaccgaca
     4561 ggaaatctgg atccagaaaa ctcatgggaa atcatgaaat tattagatcg gatcaatgcc
     4621 caaggaacta ctgtcgtgat ggctacacat aacagtacga tcgtaaatac gattcggcac
     4681 cgagtaattg ctatcgaaaa cggccgcatt attcgagatc aagcggaagg ggaatatgga
     4741 tacgatgatt agaacctttt tccgccattt attagaaagt atcaagagtt tgaaacgtaa
     4801 cggctggatg acagttgctt cagtcagtgc ggtaacgatc acattgactt tagtcggtgt
     4861 attcatggca gttatcatga acgcaactaa attagctcaa gatatagaag gaaacgttga
     4921 tgtctctgta ttcgtagata tcggtacaaa agacgaagag atgaaaaagc tagaaaaaga
     4981 attgaatgac ttagatcatg ttaaaaaagt tgaattttca agcaaagaac aggaattgaa
     5041 aaaaatccaa aacgaaatgg gtgattcctg gaaattgttt gaaggtgaca ataacccgtt
     5101 atatgatgtg tatgtagtaa gtgctactga tccttcttat acaaaacaag tagcagaaga
     5161 agcagcagat ttgaagaatg tttctcgttc tgattacggt ggagcttcct cagatcggat
     5221 tttccaaatc gctggtgcag ttcggacatg gggattagtt gcagcggcat tgctgctgtt
     5281 cgtagcgatg tttctcattt cgaacacgat tcggatcacg attttgtcta gacagcgtga
     5341 aattcaaatc atgcgtttag tcggtgcaaa aaatggatat attcgctggc cgtttttcct
     5401 tgaaggtggc tggatcggtc tgatcggtgc gattcttccg attttgatca tcacatttgg
     5461 ttatgcatgg tttttccaat tgatcaatcc atcattatta cgttcccatt attcattgat
     5521 tcatccacaa aatatcgttt ggaaaatcaa tcttttaatg gtcggcattg gtgtgatcat
     5581 tggttcactt ggctcgatca tttctatgag acgtttcttg aaattctaat accacaatat
     5641 aaataaaatg agaaagttgg acagtttcgt tccagctttc tttttttatt ggctaggaga
     5701 atagaaggaa gcggcttttt tccctttact ttaaaaaaca acttgggtat tcttaagttg
     5761 gtagaagaag aggtgaagcc gtgaaaatta tcgttgacaa tttttttacg atccttttga
     5821 ttatgaatat tttgctttcc tttatcatcg tttttcgaga aagaaaggaa actgctcaaa
     5881 catgggcatg ggtgctcgtt ttgatgttca ttccagttgt aggatttatt ttatatattt
     5941 ttttaggaag agggatttcc aaagagaaaa tctttgattt gaagatccaa gggaaaatcg
     6001 gtaagaacct ggaaattgaa gaagacaaac aggcattgat gcgagggctt tatcctcatc
     6061 cgcctactgg acaagtagat gtgaaacaat tgatctatat gctcacggta tttgaaagta
     6121 cgctctatac gactaaaaat gaaatcactt tatttacaga tggtcgtgaa aaattcgatg
     6181 cgctgattga agatatcaag caggcagaag accatatcca cttccagtat tatatttatc
     6241 gcagtgatgc attaggtgaa gaagttcgag atgcattgac agaggcggca agacgagggg
     6301 taaaagtcag agtccttctt gatgcatggg gttccacaca agtttcttcc agcttttttc
     6361 agaatttgaa aaaagcaggc ggagaagtcg catttttctt tcctctattt gttccgtata
     6421 ttaatcctcg gatcaattat cgcaatcatc gtaaaatcgt agttattgat gggaaaatcg
     6481 gatatacagg tgggttcaat gttggaaatg aatatctagg attggtaaag aaatttggtt
     6541 attggcgaga caatcatttg cgtatttacg gagaagcggt gtataccttg caaaaccgtt
     6601 ttttgatgga ctggaattct cagcatacaa aagaagtagg ctattctccc aaattttttc
     6661 cgtccattca ctctactggt gatatagctg ttcaaatcgt gacaagcgga ccagataccg
     6721 aacatgaaca aatcaaaatg acttatttga aaatgatttc gttagccaaa cgggagatat
     6781 tgatccaaac gccttattac ataccggatg gatcgattca tgaagcactg aagttggctc
     6841 ttctttcagg cgtaaaagtt catatccaaa tacctaacaa accagatcat cttctcgttt
     6901 actgggcaac gtattctttc gctgcagagc taatcgagta tggagcaagg atcgaaacat
     6961 atgagaatgg atttattcat gcaaagacga tgattatcga tggtggcatt gtttcagttg
     7021 gctcagcaaa tatcgatgtt cgttcatttc gcttggactt tgaggtcaat acactggttt
     7081 atgatgaacg aatggcttca cgtgtaagaa aagctttttt tgaagactcg aaaatttcta
     7141 cgcatctcac aaaagaaatg tatgaaaacc gaggaataat catcaagatg aaagaaggat
     7201 tagcaagatt gatttcgccg ctcctttaaa acataaaaag gacatgagaa ccatcaacag
     7261 atggagatct cgtgtccttt tctaacgggg ccatttctga gagtacgtca gaaatggaga
     7321 aagcgtctcg catgagcgaa attaactttg gggtatctct tgttttttgg ggaaagcaag
     7381 aaataagcta atatatgcta ttaaaagaga cacatttcaa agccagtata gcatagaaac
     7441 ttttaatgag atagtttttt ttacaattat gagaatttat tttgttattt aatgattttg
     7501 attcgttttc atcaagtaat gaaaatgata aaataaacat actttaggat taaaggagat
     7561 ttctaatgaa aaaacttaaa ttattcttac caataatagg attagctatt cttttatcag
     7621 gctgttcaaa gtggattgat cgaggagaat ccattacggc tgttggctct tctgctttac
     7681 agcctttagt agaaacagtt gctgaaactt accagacaga aaatcctgga aaattcatca
     7741 atgtacaagg gggcggaagc ggaactggtt tgtctcaagt acagtcagga gcagtcgaca
     7801 tcggaaactc tgatttgttt gcggaagaaa aatcggggat cgatgcctca gcattagtcg
     7861 atcatcgagt agcagttgtt gggatcactc ctattgtgaa caaggatatt ggcgtaaaag
     7921 acatcagtat ggaaaacctg aaaaaactat ttacaggcga gatcaaaaac tggagtgaag
     7981 tcggtggaaa agatcagcca gtagttatat tgaaccgcgc ttcaggaagc ggaacccgta
     8041 gcacgtttga aaaatgggta ttggacggag aaacagctgt tcaggcacaa gaacaagatt
     8101 caagcggaat ggtgcgccag atcgttgccg acacacccgg tgcaatcagc tatgttgctt
     8161 tttcttatgt ttccgatgat gtagatacgt tgaatgttaa cggtgtagaa ccgacagatg
     8221 aaaatgtgac aacgaacaaa tggacgattt gggcatatga gcatatgtac acaagagatc
     8281 atccaaaaaa actgacaaaa gaatttttag actatatctt atctgatgaa gtacaagata
     8341 acatcgttgg agaactaggt tatatccctg tttctcatat gaaagtcgaa cgtgattggc
     8401 aaggaaatat cgttaaagag taatctttac acaaaattta caaacttagc taaactttta
     8461 catgttatta aaaccaaatt gatgttcaat ttgtacactg agtgagtatg ttaaacctga
     8521 ttaaggggga gtcatctgtg gaagatgtga aaaaagtatt attaacaaaa tcaaaacgtt
     8581 caaaaatgga acagcgtgga aaattcatca gttttttatg tatcgcgctg atcgtgttgg
     8641 ttgtattatc tattttttat tttgtagcta gtaaaggatt ggccaccttt tttactgata
     8701 agatcaatgt atttgatttt ctgtttggta cagattggaa tccaagccaa atcggagcaa
     8761 atgggaaacc aatggtaggg gcattgccta tgatcacagg ttcgtttatt gtgacctttt
     8821 tatctgctat cgttgccacg ccatttgcca ttggtgcagc agtattcatg acggagatct
     8881 ctcctaaaaa cggaacgaag atcttacagc cggtcatcga gctgcttgta ggtattcctt
     8941 ctgtcgttta tggttttatc ggtttatcag ttatcgtgcc ttttgtcaga agtatttttg
     9001 gcgggactgg gtttggtatc ttagctggta catttgtgtt gttcgttatg atcttgccga
     9061 ctgtcacaac gatgactgtt gatgcattaa aggcagtgcc acggcactat cgggaagctt
     9121 cattagcgct tggtgccaca agatggcaaa caatctacaa agtcgtgcta cgagcagccg
     9181 tgcccgggat tttaacagca gtcgtttttg gtatggctcg tgcgtttggt gaagccttag
     9241 ctatccaaat ggtcatcggt aatgcagcat tgatgccaac aggtcttact actcctgctt
     9301 ctacattgac gtccatctta acaatgggaa tcggaaatac aatcatggga acagttgaaa
     9361 ataatgtgtt atggtcatta gcgctgatct tgctattgat gtcattattc tttaatatcg
     9421 tgatccgtat gattgggaag aaaggagagc tgaaataatg aacgcaaaac gctcagataa
     9481 aattgctaca ggtgttttat atatagtttc ggggatcatc gtattgattt tagcagcttt
     9541 actactttat atcttagttc ggggaatacc gcacatttca tggtccttct tgaccgaacc
     9601 ttccaaagcc taccaagaag gcggtggaat cgggatccag ctatttaact cgatttattt
     9661 gcttttgatc acgatgatca tcagttttcc tatttcttta ggtgcgggta tctatctttc
     9721 agaatatgct ggaaaaaact ggctaacaga tgtgattcgc acgtcgattg aaatcttgag
     9781 ctcgttgcct tctgtcgttg tcggtttatt cggtttcttg gttttcgtta ttcaattcca
     9841 gtatggtttc tcgattattt ctggtgcatt ggctttgact gtattcaact tgcctctttt
     9901 gactagaaca gttgaggagt ctttacaagc ggtccattac acacaacgag aagcaggatt
     9961 ggccttaggt ttgtcacgct gggaaacagt gataaaagtg gtggttccag aagcgacacc
    10021 agggattttg actggtgtga tcctaagttc tggaagaatt tttggtgaag cagctgcatt
    10081 gatctatacg gctggacaaa gtgcacctgc gttagatttt acgaactgga atccattaag
    10141 tgtttcaagc ccgatcagca ttttccgtca agctgaaaca ttagctgtcc atatttggaa
    10201 gatcaataca gaaggcacga tgcctgatgg tgtttctgtt tcggctggcg cttctgccgt
    10261 gttgattatt gctgttttac tgttcaactt cggcgctcgt gcgattggca aacgattata
    10321 ccgaaaaatg acttcagcct aaggagaaga agcaaaatga aaaaatataa ctggaatgaa
    10381 acaaatgtca tcaccatgcc aaaagagaaa gaagtagcac tatatacgga tgatctacat
    10441 gtctggtatg gcgacaatga agcaatcaaa ggagtggatc tggaatttga aaaaaataag
    10501 atcactgcac tgattggtcc ttccggctgc ggaaaatcta cgtatcttcg ttcgttgaat
    10561 cgaatgaatg atgggatcgc taatacgaaa atcactggga aaatcatgta tcaaggcgta
    10621 gatattaatg cgcctcaagt ggatgtttat gagatgcgca aacggattgg catggttttt
    10681 caacgtccta atccgttcag caagtcgatt tatgacaata ttacatttgc tttgaaacag
    10741 cacggcgaaa aaaacaaaaa gaaattagat gaaatcgttg aaacaagctt gaaacaagct
    10801 gccttgtggg accaagtaaa agacagcttg gacaaaagtg ccttggcttt atctggcggg
    10861 caacagcaac gtttatgtat tgctcgtgcg atcgcgatga aacctgatat cttgctgcta
    10921 gacgagccgg caagtgcttt ggaccctatt tcgacaggaa cggttgaaga aacacttgta
    10981 aacttgaaag aagactatac gatcatcatc gtgacgcata acatgcaaca agcagctcgt
    11041 atcagcgact atactgcctt tttctattta ggaaaagtaa tggaatatga caaaactaac
    11101 aagatattta ccagaccaaa aattcaggca accgaagatt atgtgtccgg tcattttggt
    11161 taagacggag gcctcaacat gacaaaagag attatttcat ctaaagatct tcatctgtat
    11221 tatggaaaga aagaagcact aaaagggatc gatctaagct tcaatcaagg ggaaatcact
    11281 gcgatgatcg gtccttctgg ttgcggaaaa tccacttatc tgcgttcttt gaaccggatg
    11341 aatgacctga ttccgaatgt aacgatcacc ggaagtgttt tatataaaga caaagacatc
    11401 tatggtccaa aaacagatgt tgttgaacta agaaaagaaa tcggcatggt attccagcag
    11461 ccaaacccat ttcctttttc tatctatgaa aatgtcatct atggattgaa attaaaaggg
    11521 gaaaaagata aaaaagtcct tgatcaagta gttgaagaaa gcctgaaggc agcgtctgtt
    11581 tgggatgatg tgaaagacaa attgcataaa agtgctttat ctttatccgg tgggcagcag
    11641 cagcgtgtat gtatcgcacg tgttttggca gttaatccgg aaatcatctt attggatgaa
    11701 ccaacaagtg cgcttgatcc tgtttctact gggaaaatcg aaagtatgct gttagaatta
    11761 agagagcggt atacgatgat catcgtgacc cataatatgt ctcaagcttc acgtatttca
    11821 gataagacgg cattcttttt agatggtcac ttgatcgaat acaatgatac gaagaagata
    11881 ttcatgaatc cagaaaaaca agagacagaa gattatattt caggtcgatt tggataagga
    11941 ggaaacggaa tgctacgtac acaatttgaa gaagaattgc tgaatctcca taatcagttc
    12001 tacgaaatgg ggatgatggt gagcagcgcc gtgcacaagt cagttcgtgc atacatcaaa
    12061 cacgataaaa tcttagccca agaagttatc gaaaacgata taaatatcaa taacatggaa
    12121 acacgcttgg agaagaaaag ttttgaaatg atcgcattgc aacagccagt tacaactgat
    12181 ttgcgtatga tcattacggt catgaaagct agctcggatt tagaacgtat ggccgatcat
    12241 gcggtgtctg ttgctaaatc cacgattcgc gtaaaagggc agacgagaat accggaaatc
    12301 gaaaaagaga tctccgatat gtccgattat gtgaaaaaaa tggtggacaa cgtcctagtt
    12361 gcttatgtca aaacagacga aaaagacgca cgtacgatcg ccaatatgga tcaaagagtc
    12421 aatgactatt tcaatcgaat ctataatgca acaatcaaaa atatgcagga aaatcctgag
    12481 acagtcatca gtggaacaga ctacttgaat gttgccagtt atctagaacg gatcggagac
    12541 tatgtgacaa acatctgtga atggatcgtc tacttggcta cagggaaaat cactgagtta
    12601 aatacgaacc acaatgagat tttctaaact gaagctgtga ttatgccgtt tgtttaggaa
    12661 aagaggttgt gaaatcagcg gttgtgaaaa agctgttctg catcaagtaa taagaacagt
    12721 caaacaaaaa atagctcctc atatttttcg tttggctgga cttattaccg cagatgcaag
    12781 cttttgaaca ccgtttattc gggaaagggt ttgtgacaaa acatgtcaca aacccttttc
    12841 atcatttcat agaaaggata aagaatgcaa aaaaaaatta gcatcatcac tatatttttt
    12901 atttgtgtgc ttacttcatg ggttattcag catcaagccg ctttatatga agtacctgtc
    12961 atcgagatag aaaaagtacg aataaaagag gatcatcaat tgattacagg aaaactgatg
    13021 aacacggatc aaaaaggaga atcgatttat ttatcggttc cttatcaaaa aaatcaaata
    13081 gaaacagcaa atataaaagc gagacaaatg tatctagtcc aaatgcatgg agatgaatgg
    13141 cagctagtag caaaaaaaaa tgatggctgg cttttcttct tcacaagtat cttcattggc
    13201 gccatgttgc ttattggtgg aaaacagggt ctatttgctt taggaagtct catcatcaat
    13261 atcgttttac ttttactttt gttgctcatc tttactaaaa cagaagggat ttctctatta
    13321 aatatatcta tcctgttcgt cgttataggg atgattatta gcatattggc tttagatgga
    13381 tggaatagaa gcagtataat caaaatttca gctgcagtta cgagtgtttt tttagcattt
    13441 tttatttgtc ttttgacaat gaatcattgg aaagaccaag gactgcgcta tgaggaactg
    13501 aattatctga cacgaccgta tcgttctgtt tttctttcta gcgtactgat tggaacagct
    13561 ggaggaacac tcgataccgt tatcacagtc attgcgacat tagaagaatt gactaagcga
    13621 caaccagaaa taacagcgaa acagttatgg ctttcaggaa ggcaagtggg tcgagatgtg
    13681 attggctcga tgaccaatat ccttttgttc gtctatatca gtgggtcagt tccatcgctt
    13741 cttttatatt taggaaatca atggtcgttc aaagaaacga tagaacagca tttatcattg
    13801 gaatttttac gcgtcattgc tggcagctta ggaatagtat tatcgatccc tatctcggtc
    13861 ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt acttattata cttgttggga
    13921 ttttagcaat cagcgtgcta cttattggcg gtacgcaagg tatgtatatt cttttaggct
    13981 tattcatcaa tttagggatc tttttcctgt tgttattcgg ttatcatcag aagtggccga
    14041 tactggcatt gtcggtcata ggatttttat tgattgctgt ggttatctta ttttttatca
    14101 acggttataa tctaaaaatg cgtgcagctt ttgtgagtat actgatcttt ttattttgtt
    14161 tcctactatt gcttcctatc actgattttt tagcaatcca aggttttaca tcggtagaac
    14221 tagaagaatt atctggctta gataagactt tagcgattga ttttagatta cttacgagat
    14281 cgttgcttct gattagtctt agtggtgctg ttttagatgc gagtgtagca atttctagtg
    14341 ggacattcga agtatatcag gcaaatccgc aattatcttt ttaccaattg cgccattcta
    14401 gtttcgctat tgctaaaaaa atattggcgt caacagtaat gacacttctt tttgcattca
    14461 tgggaagttc tttagcgttg atcctctggt ttattgattt agacattcct tttcagcaag
    14521 tgatcaatga aaaatcattt gttctagaat atacgatggc tattcttacc acattgtccg
    14581 cactacttgt tcttccttta acctgcgtga ctacagcata tctctttaca aaaaagaaag
    14641 aacaactcaa gatggagtaa aagttacaac ttaagtccta tgacattttt actgaatcag
    14701 tgcataatat ggatgtaagg aaaagcttgc aaacaatttt gcttaaggag gcaatttcca
    14761 tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg aatcctttcg actgaagaag
    14821 gcttagattt attagaaagc atggcaactg aaaaagacga aaagcaaatc aaaaaagaag
    14881 cagacaaggt aactgctagt cataaagaaa aagatcaggc aagtcaattg atcgataaac
    14941 ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc aaaggaaaga gaaagacaag
    15001 atcaagaaaa cttagaaaag atcttagatg aactagcaac tgaagcgaat aaaacttcgg
    15061 cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa ggcagcacgc aatgagaaac
    15121 aggaagcatt gatgcagctg aatacaaaag aagaattagg cgagttgaca gaagaagaat
    15181 tagcacagcg tcagacgtta gaagcagaaa tcaaagaatt agaagctagt ggcgatacac
    15241 tgttagaaga acaattgaaa ttagaagcag aattaaaaga tatccgtaag aatcaatggt
    15301 ctgagaaaaa agaaacgttt actgataagt ttgaattgcc agatgattgg aaagatcaag
    15361 caacagatac actgaatcaa gtaggcgaaa aaatgtcaga agctggatca cagttaggca
    15421 aattcttgaa gaaaacattc caaactgttt cagaaacagt gaatgacaac atggaatgga
    15481 aagatgtcag cttacgtgtc cctgggattg cgacaacgaa atttgagcat gaattttact
    15541 atgaagctcc tgcagcaagt atccttgata tcaaggcagc aaacggaaac gtgacgttaa
    15601 aaacgtggga cagcaacgat gtgaaagttg aagctaagat caaactttac ggaaaaatgg
    15661 gtgctgaacc atttgaagca ttctctgaac ggagccagat tgaggttaat gaagatcata
    15721 tctccttcca aattccaaat aaacgtgtgc gtgcagacct tgttttctac ttacctaaac
    15781 gagtatacga ccacgcagct attaaacttc taaatggcaa catcatgatt gaaacattgg
    15841 aagctaaaga tatttataca aaatctacga atggtaacat catcgtggac caattaactg
    15901 caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga tattcgaaac gggaacatct
    15961 tagattctat tatcgaaaca gtaaatggta cagtgacatt tggtgcgaca gcagaaaacc
    16021 tttctgtgtc tttggtaaat ggtgatgtac gtcttacgat taaagaagat aatttgaaaa
    16081 aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc tttaccagat ggcatcggtt
    16141 tggaaggaca tgctaaaaca agtcttggaa gtatcaacag ccgtctgtcc aattacgaag
    16201 tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca attccgtcgt gtttcagatg
    16261 gtgaaatcgc gcaagtgcaa ctttcaacga caacaggaag tatctattta aaagatacag
    16321 ataaataaag aggttgtgaa agagatgttc agccttgagc aataagaagc gattttgtaa
    16381 aatagcttcc cctattttac aaaattgtgc cttattgccg atagtctatc tcttgaacac
    16441 cgtttaccaa gagattatga acctgccaat tatcgccgag tcacaaggaa taatttcaga
    16501 aaaagctcgt cacgttttat gaatatggta atttgtgacc gagaattttg cagaaagaca
    16561 ccgattaaga agaaggttgt cacaacgatt ttcttcattt aaaatagtag aacagaggtg
    16621 agacatgatg aaaaaaaagt tgacaaaatc taataaaaat gttgttttga cagggacttt
    16681 agcaggaata ggagaataca tcgggattga tccgactgtt atccgtgtgc tttatgtatt
    16741 cctgagctta gttgcttttg gaagtccaat tattttatac attttattag ctttgattat
    16801 cccatctgcg cgttctgcac gaacaaacta tggacataac aatgcatatt atgaaaaaaa
    16861 caattatcgt gaacaaactc gtgaacaaac aaaaaaacgc aaagaagcgg aaaaaatcga
    16921 tgatgacgaa tggagtgact tttaatgtcg tactttcaac gtattttagt taatacgtta
    16981 acattcatat cattggctgt tctttttcct gcgatggtcc atgtgagcag tatttggacg
    17041 gccgttcttg ccagcttcgt attgtctgtg ttgaatatgt ttgtcaaacc gattttgacg
    17101 atactgtccc tgccctttac attagtcaca ttgggattgt ttacgtttgt tatcaatgca
    17161 gcgattctta aaatgacctc cttttttgtg ggagcagaac attttagttt ttcttcattc
    17221 ggtgcagcgt tgttagtagc tatcttgatg tcgcttgtca atgtaattgt cagcgaacat
    17281 aacagtagaa aataaaagaa agctgtgccg aactatcggc gcagtttttt tatttagaat
    17341 caggaatgcg ttttctatta cattggtaat taaataaaaa cgtttttttt aattggtagt
    17401 acttttggct agacaatgat aaaatgatag agaacactct aatagaaatg aggtagcatg
    17461 aatgtcagaa gttgtaaaag ttagccaatt agtgaaaaaa ttatctttgg aaatcgtgac
    17521 aggcgacgag aaaagcttag accgtgtcat cgtaactggg gacatctcac gcccaggact
    17581 agagttgact ggttatttta attattattc ccataatcgt ttacagctat ttggcagtaa
    17641 agaaatcagt tttgcggaac ggatgatccc agaagagcgt ttgatcatca tgagaagatt
    17701 atgtagtgaa gatatgcccg catttattat ttcaagagga ttaccagctc cagaagaaat
    17761 gatccaggca gcaaatgaaa atgggatagc tgtactgcgt tctcccatct caacttctcg
    17821 tttattagga gaattatcca gttatttaga tggtcgcctt gcccctcgaa caagtgtcca
    17881 cggtgtttta gtggatgttt acggattagg tgtactgatt caaggtgaca gtgggatagg
    17941 aaaaagtgaa acagcgttgg agttgatcaa acgcggccat cgattgatcg cagacgatcg
    18001 agtagatgtt tatcagcaag atgaattaac ggtgatcgga gaaccgccta aaatcctgga
    18061 acatctgatt gaaatccgag gaatcgggat cattgatgta atgaatcttt ttggtgcgag
    18121 tgcggtacgt ggctctatgc aagtccagtt ggctgtttat ctggaagcat gggcaaaaga
    18181 taagaaatac gaccgtctgg gctctgaaga cacgaccgtt gaaatcgcgg aagtgggcat
    18241 tccacaaata aaaatccctg tcaaaacagg acgaaacgtt gcaatcatca ttgaagtagc
    18301 cgcgatgaat ttccgtgcac gtacaatggg ctttgacgca acgaaaacat ttgaagaaag
    18361 attaagtcgc ttgattgaag aaaactcagg caacgactag caaggagtga caatatgtta
    18421 gcacaaatca atcgagttgc ttttgagctt gtagggattc ctatctactg gtatgcattg
    18481 atcatcgtat caggtatcat tatcgctatg tggcttagca gcagagaggc tgttcgggta
    18541 ggattaaaag cagatgatgt gacagatttt atgttgtggg gattgcctat ctccattatt
    18601 ggcgctcgct tatattatgt tttatttgat cttccccagt atatagctga tccaatccaa
    18661 atattcaata tccgttcagg aggcttagct atttatggcg gactgattgc aggcgcgatc
    18721 acgctgtatt tttttactaa atatcatttt atttctttgt ggactttttt agatatcgct
    18781 gcaccaagtg tattattagc acaggcaatc ggtcgttggg gaaatttcat gaaccacgaa
    18841 gcatatggac cagacacgac gcgaagcttc ttagaaagtc tgcatttacc gcaattcatc
    18901 attgataata tgtatatcga agggatctat cgtcaaccaa catttcttta tgaatcactt
    18961 tggagtttga ttggatttat tttgcttttg tttttacgaa agaaaccaca ccttttgaaa
    19021 aaaggcgaag tccttttatg ttatctaatg tggtatagtt tcgggcgttt ctttattgaa
    19081 ggattacgta cagacagtct ttacttttta ggtgtgatcc gtatttctca attattatct
    19141 gctgtgttat ttgtcggtac attgattcta tttatttggc gcagaaaaaa aggaaattta
    19201 ccggattatg accgctcatt aggaaaaaat caacaaatta tctgattttt agagcgttaa
    19261 aaatgaaaaa aatttcagga gtggtaaaat gaattataga gtagcggttt taggaccagg
    19321 ctcttggggt actgctttgg cacaagtcct cgccgaaaac ggacatgacg ttcggatttg
    19381 gggacatcgt gcttcacaag tagaagaaat caacacccaa cacacgaatc aacgttattt
    19441 accagatcat tatctacccg attccatcca agcattcacc aatatggaag atgcagtaaa
    19501 agacgcagat gcggtgctat tcgtcattcc aacgaaagca atacgttctg ttgcaaaaga
    19561 actagtgcct aaattgaata caaagccagt gatcatccat gcaagtaaag gattagaaca
    19621 gggaacacat aaaaggatct ctgacatctt gttagaggaa atcccagcag aaaaaagaaa
    19681 agctgttgtt gttctttctg gaccaagcca tgcagaagaa gtagcaaaac atgacatcac
    19741 tacgatcaca gctgcaagta cagatgcaaa agcagcaaaa tatgtacaag agctgttcat
    19801 gaataattat tttcggatct ataccaatcc tgatgtgatc ggtgtagaga tgggggcagc
    19861 tttgaaaaat attattgcta ttggtgctgg cgctattcac ggcttagggt ttggcgataa
    19921 tgcaaaggca gcaatcatga cgcgcggatt agctgagatc agccgtttag gtgtagcaat
    19981 gggtgccaac ccgctgacct ttatcggatt aagcggaata ggcgatctag tcgttacttg
    20041 cacaagtgtt cattctagga actggcgcgc aggcaacttg ttaggacaag ggcagcccct
    20101 agaagaggtg ctagccaata tgggaatggt cgttgaaggt gtgaatacta cccaagcagc
    20161 gatggaacta gcccaaatga tggaaatcga aatgcccatc actcaaacaa tctatgaagt
    20221 gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa atcatgttac gtgatggaaa
    20281 aaaagaaaat gaattctcat taacaatttt ttgaaggagc gaatgtatat gaaagttaaa
    20341 aaagcagtta ttccagcagc aggattagga acaagatttt taccagcaac aaaagctatg
    20401 gcaaaggaaa tgttgccaat tgttgataaa ccaacaatcc agttcatcgt tgaagaagcg
    20461 ttgaaatcag gtatcgaaga catcttgatc gttacaggaa aagctaaacg accaatcgaa
    20521 gatcactttg attcaaatat tgaattagaa acaaacctaa gtgaaaaagg aaagaccgaa
    20581 ttattgaaat tagtagaaga aacgacagat gtcaacttgc atttcatccg tcaatctcat
    20641 ccaaagggtt taggacatgc cgttcttcaa gcaagagcct ttattggaaa tgaaccattt
    20701 gtcgttatgc taggcgatga tataatggaa gacgaagtac cattgacaca gcaattaatc
    20761 gatgattacg aacgtactca tgcttctacg atcgccgtta tgcaagttcc tcatgatgaa
    20821 acttcaaaat acggaattat caatccagga gaagtattgg aagatgggtt atacaatgtc
    20881 aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa gtgatttagc tattattgga
    20941 cgttacttat tgactccaga aatttttgat gtattgtccg agcaacaacc aggagccgga
    21001 aatgaaatcc aactaactga cgcaatcgat acattgaata aaacacaacg cgtatttgcc
    21061 cgtaaattca caggtaaacg ttacgatgtc ggtgacaaat ttggttttat gaaaacaagt
    21121 attgaatatg gtctaacaca tccagaagta ggtgcacctt tacgtgagta catcctgtct
    21181 ttaggtaaaa cattagaagc agaaggaagc aagatcgatc ataatccaaa aggaataaca
    21241 gaaatcaaag aagaaaagaa aaaataaccc ttttttctaa cgagcttcag ctgctaattg
    21301 aataaaaagt agttgaagct cgtttttatt tgtttaaaaa acacgaaaat gctatcctta
    21361 tagaaaagag agacaagaag ggagtttgat catgtcagga ggagaaattg caggattgat
    21421 cgcagcaatc gcctttgcga tacttgtcgt ttttatcgtt cgtgtgttgt tgcaagtttc
    21481 taaaacttta gaaaaagtag atcagactgt agcagaagca aatacgacga tcgaaatcgt
    21541 cacaaaagac gtagatcttt tgtctagaca agtagaaggc ttactggtca aaagtaatga
    21601 actgctcttg gatatcaatg gaaaagtcgc aacgatcgat ccattgttca cagcagttgc
    21661 tgatttaagt gaaagtgtat cagaacttaa tcattctagt aaaaacatcg cgacgaaagt
    21721 cggcggaatc ggtaaaacga cagctaaagc gacggttgcc ggaaaagtcg gtgggacagc
    21781 gatgaaattt tttaaaaaca aaaaacatac tgaaacgaca ggaggaaaat aaaaatggct
    21841 aaaaaaggtg gatttttatt aggtgcagta gtcggaggca cagccgcagc aatcgccgcg
    21901 ttattgttag caccaaaatc tggtaaagaa cttcgggatg aattagctgc ccaagcggat
    21961 gatttgatgg atatggcttc agattacaca gatatggcga aagaaaaatc gactgagtta
    22021 acaggtattg caaaagaaaa agcgcaagat ctttctcaac aagcaggtga tctagcaggt
    22081 agtgtcaaac aaaaagtaag tgaatcagca gatgaagcag gaagtgaatc tgacgatatc
    22141 ttgttaaatc ttaaagaaca atcagctgac ttatctaatc gtttcaaaaa aacggcagaa
    22201 gatatgaaaa aacctgcaga tgaattagga agtattgcta aagatacttc agaagatatc
    22261 atgattgatg taaaagacgc tgctggtaaa gcaggagata ctgtcaatga aggaatgtca
    22321 gaagcaaaac cagttgcaga tgactcaaaa gaaatgatga aaatggcaaa agaagatgcg
    22381 aaagaagcta aaaaagaaat aaaagataca atgacagatt catctacaaa tcactaaaat
    22441 cacaaattta tagaataaaa aggtgggagc ggactagctc tcaccttttt tactcttatt
    22501 tttctaaaac aagtaattct tttggtgtag tcgtgaatgg cacacagcca tcgcttgtca
    22561 catatacaca gtcttcgata cgtaccccta ctttttcagg aatatagatt cctggttcaa
    22621 gagagaagca catcccttct tcgattacta agtcatttcc ttctacaagg gaagggaatt
    22681 cgtggacggt tgttccgatc ccgtgaccta aacggtgatt gaaatattcg ccgtagccat
    22741 agctgttgat cacgtttcgt gcgatttgat caagttcacc tgctgtcaca cctggacgga
    22801 cggcttcttg tgcggcaagc tgtgcttcaa gagtgatgtt gtagattttt tcttggaagt
    22861 cggttggttt ttgataagca attgtacgag tagcatcact gcaatagcca ttccaaacaa
    22921 cgccaagatc gaataacaca agttcattcg gtgaaatctt cgtatttcct ggtgtaccat
    22981 gagggcttgc agcatttgct cctgcaagga ctaatgtgtc gaaagacatt tgtgaaacac
    23041 cttgtttttt caattggtat tcaatttctg caatgatcgc ttgttcagtc acgccttctt
    23101 tcactgcttt aaagccaatc tcaaatgcaa catcggccca tttaccagct tcgatcagtt
    23161 tgtctttttc ctctagagtt ttgatcagtt gtagacgttg gatgatggaa gttagatcag
    23221 tgtaagaatc cgttcccgtc aagatatcag agatagcatc gaagcgctcg acagtcaagg
    23281 cgttcttttc aatccctaca ttgccaggct gaccatagcg agcacgaagt tcttttccga
    23341 tgatctccca agggttttcg ctgtctaaat agccacggac atcatacgcc caactactgt
    23401 tttgagcatc ttcaacttcc aaagcaggag tgaaaaggaa aggatcttgt tcaagaggga
    23461 taaaaagagc tagtacacgc tcatgaggat cactttcgta tccggaaaag taagcgatgt
    23521 ggccagggtc gctgatataa gccaattcga cttggtgttg tgccatccat tgacgtagtt
    23581 cattgatttt tacgttattc atttggcaac ctactttcta aattaatttt gtattcaata
    23641 attgtaccac tgatttatta aaagaccaac gttctaaaaa ctgaaaaaag taaaaatttt
    23701 catgaaaaca gagataaaac ggttgcaatt cttgagatta tttgatattc tattttagaa
    23761 atgtaagacg tattttcaga aaacgtataa aatatgaata ggagaacaca aatggaaaag
    23821 caaacaatca ctatctatga tgtagcgaga gaggcaaatg tttcgatggc tactgtttca
    23881 cgtgtcgtaa atggcaatcc aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtt
    23941 atcgatcgct tggattatcg tccaaacgca gttgcgcgtg gcttggcaag taagaaaaca
    24001 acaacagttg gtgtaatcat ccctgatgtc agcaatatgt tctttgcttc tttagcacgt
    24061 ggtatcgatg atgtggcaac aatgtacaaa tacaatatta tcttagcaaa ttcagatggc
    24121 aacgaccaaa aagaagtcaa tgtattgaat aacttactgg ctaagcaagt ggacggcgtg
    24181 atcttcatgg gtcaccatat cacagacgaa atccgtggag aattttcacg ttcaaaaact
    24241 cctgttgtat tagctggctc tattgatcca gatgaacaag taggtagcgt aaacattgat
    24301 tatgtatccg caacaaaaga tgcagtaaac aaattggcaa aaagcggcaa taaaaaaatc
    24361 gcttttgtca gtggtgcgtt gatcgatccc atcaatggac aaaatcgtct gaaaggctat
    24421 aaagaagcat taagcgaaaa taacctacca tatagcgaag gattgatctt tgaagcacaa
    24481 tacaacttca aagatggtct atctctagtc gatcggatcc ataatagcgg tgcaacagct
    24541 gtctatgttt cagatgatga attagccatc ggtattttgg atggtttatt agaccgtggc
    24601 gtgaaagtac cggaagaatt cgaaatcatc acaagtaaca attcactact gacagaagta
    24661 gctcgtcctc gtttaacaag tatcacacaa ccattatacg atttaggggc ggtatctatg
    24721 cgcctattga cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct
    24781 tatggaatcg aagaaaaagg atctacaaaa taataaaagg acaaccattc agaggttgtg
    24841 tgcctgccgt ttaactctgc gttacaagga gcaatttcaa aaaacagcat ctcatgtttg
    24901 atgaatattg tgactcgtga ttcggagttg gcaggagaac accgtttatt tgggaaaagg
    24961 ggttatgaca aaattttgtt ataatccttt ttttgtgtct taaaaaagag agaaagtggt
    25021 tgaaattaca tttatcgtat ataaaacaat ccgtgtatag atgctgcatt ttgttcacca
    25081 ccatcaaaaa aataagggac tgtgacaata ctattgtcac aatcccttat tactgtgtat
    25141 tctattaaat caatcatctt tctcatctga ttctttagtt gcttgattcg ctcttgcata
    25201 agctgaagcg gtgttccagt aatctgtata gttttcatcg gtaccgccaa cgccaaattt
    25261 aaaggcgctt ttcttcggtc catttttcgc ccatagcgat tcaactgttt ttcctggtgt
    25321 ttggatggat tttttattga cagtaacttt tccaggtgtt tgaccagtaa atccagatac
    25381 ttttttcttc atcacatcag atgataattc gaatttctca tcagtgccaa aaatgtcagg
    25441 ttttgcttgg taagcttgat tgatcagatt ggcaagataa ttagccgaac gtatccttgc
    25501 tttgctgtcc attccagtat tgtcatcgtg ccctgcccag ctactgatgg taatagacgg
    25561 agtagagaca attagccatg aatctcggta ttcattggta gaaccagttt ttccgaccca
    25621 atctgctttt cctagatttc cgtttaagct tgagatggca tctttgaatg gtgtagtgat
    25681 ttttgcattg atcacactac gcatcatgtc attcatgatc gatgccgttg cttccgaata
    25741 gatccggaca gagttgcttt cgtgtttata aattacattt cctgcgttat ctgtgatgga
    25801 atcgatgata tagccttctt catagacgcc gccgtttgct aaagcctgaa atccgttggt
    25861 ctgttgaagc gtcgtgacat ctactgtacc taaaggagca gattcaacgc cccaattgtc
    25921 atttgccggg tagttcatct ttgccaagta gtgctcgtaa gcatattgtt tcgatccacc
    25981 ggaattcaac acatcttgat ataagtgata agccgggata ttgttggacc attctagtga
    26041 ctcacggacg gtttggaagg tgttcgatcc ttcgttagta gcattgacga ttttctcgcc
    26101 agcattttct ccttcttgcc aagttgttgc ataatctgat acacgggatt ctgatccgat
    26161 aagccccatg tcgattgcag gtccataaac aagaactggt ttgattgatg agccagcttg
    26221 acgttctgta tcgaaggcat ggttattttg attctgactg taattccgtc cgccaacgaa
    26281 tccgtagatg cggccagttc tgttatccat caatacattt ccggtttcta cttgactgcc
    26341 agcaccatca tctaacatgt atccataatt ttcaacgcca ttttgcaatg cagcatagac
    26401 atctttatcg attgtcgaat ggattgtata tcctttattt tggatcgttt gctgtgcctt
    26461 ttgataataa gcgtcactga ccgatgaatc agacatatca gcgttgtctt tttcagccaa
    26521 ttgttgtgca atgattcttg ttgcttcatt catcacagta taatacaaga attctctgtc
    26581 agtctgttct gcgatttgtt gtggtagaaa gtcttttgta aggtcatagg cttttgcttc
    26641 ttcgtattgt tctttcgtaa tttggccttc acgatacata ctgaataaaa caaaatcttt
    26701 tctggcaagg ccggcagaaa gatcttcttt tagcgcgcct gtgttcgtgt atggactata
    26761 cgtgatcggg ctttggggta atccagcgat ataagcagct tgcggcagcg tcacatcttt
    26821 tgcatctaca ccaaagattc cttttgcagc ttcctggact ccagcaatgt tttgtccctt
    26881 attattacga ccaaatggtg agacattcaa atacgtagca atgatctcat cttttgaaaa
    26941 gtatttttct acttgtgcag aaagaatgat ctcgtttgct ttacgtttga atgttgtttc
    27001 atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa gttgatccac cagaagagcc
    27061 aataccagta gcttcagata ctaatgctcg taaaacagct ttaggaacgt aaccttggtg
    27121 tttatcgaag tattcatcct ctgtgctgat aattgcagtt ttcaataaag gtgaaatctt
    27181 atcagatgaa atagtcgtgc gcatcagatc tgtctggatt tttgatatct ctgtattatc
    27241 tgcataaaca atctttgacg tttctgtaat gtttcctagt tctgtctgca gtgctttttt
    27301 agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag ccagcgccta ttccaatacc
    27361 tagagctgcg aacatcaaaa gaagaacagt aataaaaaca gtcaatgatt gaaaaactct
    27421 aaaaataatc ttaggaacaa accatttttc tttttttctt ctattctgtt tttttcgagt
    27481 atttttgcgt ggcaaaaggg tcacctcaac taatggtttt atttcgctaa ttatagcaaa
    27541 taatagaaca aaaaaacaga caaggctgtt tttggcagga atttcttttt taaaagagtt
    27601 aaaacgtgag aaaatgatct ttattataaa agagttctta agcttttctt tagagaaaat
    27661 ttcagttact atggagaaca gggcaaagaa aaatcaatca ttttctttgt atatcatctt
    27721 accaatatat aggaattgtt agtaacaatt gcctttcaag gaggaatcca tccatgttat
    27781 taggctcaca cgtcagcatg agcggaaaga aaatgctgtt aggagcagca gaagaagcag
    27841 ccagttatgg atcttcgact tttatgatct atacaggcgc ccctcaaaac actagaagaa
    27901 aagccatcga agagatgaat attgatgccg gaaaagcgta catggcagaa aaaggtctaa
    27961 caaatatcgt cgttcatgcc ccttatatca ttaatttggg gaatacgatc aaaccagaaa
    28021 tgtttccttt tgccgtccaa tttctgagag aagagattgt tcgtgcggaa gctttaggcg
    28081 ctactcaaat tacacttcac ccaggtgctc atgtaggggc aggaacagaa gccggaattg
    28141 ctagaattat tgaaggcttg aatgaagttt tgacaaaaga acagacggca caaatcgctt
    28201 tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac atttgaggaa ttagcgcaaa
    28261 tcatcgatgg agtaaaattg aatgaaaaat tatccgttac ttttgacacc tgtcatacga
    28321 atgatgccgg ttataatatt aaagaagact tttcaggtgt aatggaagag tttgataaga
    28381 tcatcgggtt agatcggctt aaagtactcc atatcaatga ttctaaaaat ccgcaaggtt
    28441 cgcataaaga ccggcacgcc aatattggtt ttggaacaat cggattcgac acgctgaata
    28501 agatcgtcca tgatccaatg tttgacaata tttcgaaaat cttggaaact ccctatgtag
    28561 gcaaagataa aaagaaagct tacgcaccat atggaaaaga aatcgccatg ttcaaagcgc
    28621 aaacattcga tccagaagtg tttcctgagt tattagcaga agaagtgata ctctaagatt
    28681 ttctatctga cagcagcaaa aaaggaccag gcacattgcc ttgtcctttt ttgtttaaga
    28741 aacaaaggag tactttttgc agtctttttc atcatgcatc ttctcccccc tctttttaaa
    28801 tttagacaaa caaggtatac tggtagaaaa gtaaaaaaac gaaaagtttc acaagcaagt
    28861 ggcaaaacgt gattggaagg acgaaaagat gctgaacact ttaaaaccct tgatcaacca
    28921 actaaaaaag aaacatacat actttattct tggcatagcc ctattattta caattattcg
    28981 tttgtattta tatagtaaag caatctatgg aacagacatg tcggcaacct atgatgacca
    29041 gctgctgatt cgctatgctc aaaatatttt ggacggtaaa tggctagggg agtatacgac
    29101 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
    29161 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
    29221 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
    29281 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
    29341 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
    29401 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
    29461 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
    29521 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
    29581 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
    29641 agttggcttt tccatcgtct atcaatggat cgaatataaa aatgagacaa catacggtat
    29701 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
    29761 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
    29821 actaaataaa gcattagccg catctcctac attccatacc gtttctaccc gtattgacga
    29881 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
    29941 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
    30001 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
    30061 aacaatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
    30121 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
    30181 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
    30241 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
    30301 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
    30361 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
    30421 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
    30481 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
    30541 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
    30601 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
    30661 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
    30721 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
    30781 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
    30841 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
    30901 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
    30961 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
    31021 ggtcatcatc agtaaaggaa tcatcgaaaa cggcaacgca aaagccaaga acacctggga
    31081 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
    31141 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
    31201 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
    31261 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
    31321 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
    31381 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
    31441 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
    31501 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
    31561 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
    31621 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
    31681 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
    31741 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
    31801 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
    31861 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcaatatc
    31921 tgttgccata atggctaatt cagtcaagat ccataaaatg acacctaatg tcttacttgt
    31981 gcgtgctcgg attgcctgtg ccaaatccat ttggctcacg attcctaatt ttgctgccat
    32041 atactgtaaa agcatagcaa tcagactaga catcaaaata actgacatca ataaatattg
    32101 gaaattttgt cctccggtga tcgaagtaga ccagttaccc gggtccatat acccaactgc
    32161 tactaacgca ccaggtccag agtaagcaag aagtgtttta aaaaaacttt tcccctttgg
    32221 tacatcaact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
    32281 atgcttatga aattgtttat tggattgatt attcaagaaa aaaccacctt tcctatttat
    32341 ctacgattac attctacacc tcaaaaagaa aaaattaaag aaaaagattc ggtacgccta
    32401 atttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa
//
LOCUS       DAJSVF010000016        43809 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSVF010000016 DAJSVF010000000
VERSION     DAJSVF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.016"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(449..2830)
                     /locus_tag="ON550_000849"
     CDS             complement(449..2830)
                     /locus_tag="ON550_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3953954.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2820..3467)
                     /gene="recU"
                     /locus_tag="ON550_000850"
     CDS             complement(2820..3467)
                     /gene="recU"
                     /locus_tag="ON550_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR3953955.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3531..4070
                     /locus_tag="ON550_000851"
     CDS             3531..4070
                     /locus_tag="ON550_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR3953956.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4160..4579
                     /gene="gpsB"
                     /locus_tag="ON550_000852"
     CDS             4160..4579
                     /gene="gpsB"
                     /locus_tag="ON550_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR3953957.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4601..4968
                     /gene="rnpB"
                     /locus_tag="ON550_000853"
     ncRNA           4601..4968
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ON550_000853"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5177..6343
                     /locus_tag="ON550_000854"
     CDS             5177..6343
                     /locus_tag="ON550_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR3953958.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGTHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6389..7885
                     /locus_tag="ON550_000855"
     CDS             6389..7885
                     /locus_tag="ON550_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR3953959.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7966..8649
                     /locus_tag="ON550_000856"
     CDS             7966..8649
                     /locus_tag="ON550_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3953960.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9294..10484
                     /locus_tag="ON550_000857"
     CDS             9294..10484
                     /locus_tag="ON550_000857"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR3953961.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10586..11539
                     /locus_tag="ON550_000858"
     CDS             10586..11539
                     /locus_tag="ON550_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR3953962.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11608..12957
                     /locus_tag="ON550_000859"
     CDS             11608..12957
                     /locus_tag="ON550_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3953963.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13140..13463)
                     /locus_tag="ON550_000860"
     CDS             complement(13140..13463)
                     /locus_tag="ON550_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953964.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13592..14755
                     /locus_tag="ON550_000861"
     CDS             13592..14755
                     /locus_tag="ON550_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3953965.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14825..16174
                     /locus_tag="ON550_000862"
     CDS             14825..16174
                     /locus_tag="ON550_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3953966.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16219..16650)
                     /locus_tag="ON550_000863"
     CDS             complement(16219..16650)
                     /locus_tag="ON550_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3953967.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17038..19680
                     /gene="alaS"
                     /locus_tag="ON550_000864"
     CDS             17038..19680
                     /gene="alaS"
                     /locus_tag="ON550_000864"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR3953968.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19880..20476
                     /locus_tag="ON550_000865"
     CDS             19880..20476
                     /locus_tag="ON550_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3953969.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAIATTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20598..21746
                     /locus_tag="ON550_000866"
     CDS             20598..21746
                     /locus_tag="ON550_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3953970.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21784..22197)
                     /locus_tag="ON550_000867"
     CDS             complement(21784..22197)
                     /locus_tag="ON550_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR3953971.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22276..22707
                     /locus_tag="ON550_000868"
     CDS             22276..22707
                     /locus_tag="ON550_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR3953972.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22806..23039)
                     /locus_tag="ON550_000869"
     CDS             complement(22806..23039)
                     /locus_tag="ON550_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR3953973.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23222..24892
                     /locus_tag="ON550_000870"
     CDS             23222..24892
                     /locus_tag="ON550_000870"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR3953974.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDKELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25039..25425
                     /locus_tag="ON550_000871"
     CDS             25039..25425
                     /locus_tag="ON550_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953975.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25412..26110
                     /locus_tag="ON550_000872"
     CDS             25412..26110
                     /locus_tag="ON550_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3953976.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26115..26579
                     /locus_tag="ON550_000873"
     CDS             26115..26579
                     /locus_tag="ON550_000873"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR3953977.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26576..27487
                     /locus_tag="ON550_000874"
     CDS             26576..27487
                     /locus_tag="ON550_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3953978.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27722..28261
                     /gene="pyrR"
                     /locus_tag="ON550_000875"
     CDS             27722..28261
                     /gene="pyrR"
                     /locus_tag="ON550_000875"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR3953979.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28275..29558
                     /locus_tag="ON550_000876"
     CDS             28275..29558
                     /locus_tag="ON550_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR3953980.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29571..30497
                     /locus_tag="ON550_000877"
     CDS             29571..30497
                     /locus_tag="ON550_000877"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR3953981.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30516..31796
                     /locus_tag="ON550_000878"
     CDS             30516..31796
                     /locus_tag="ON550_000878"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR3953982.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31801..32877
                     /gene="carA"
                     /locus_tag="ON550_000879"
     CDS             31801..32877
                     /gene="carA"
                     /locus_tag="ON550_000879"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR3953983.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32882..36067
                     /gene="carB"
                     /locus_tag="ON550_000880"
     CDS             32882..36067
                     /gene="carB"
                     /locus_tag="ON550_000880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR3953984.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36121..36897
                     /locus_tag="ON550_000881"
     CDS             36121..36897
                     /locus_tag="ON550_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR3953985.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36894..37826
                     /locus_tag="ON550_000882"
     CDS             36894..37826
                     /locus_tag="ON550_000882"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR3953986.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37823..38539
                     /gene="pyrF"
                     /locus_tag="ON550_000883"
     CDS             37823..38539
                     /gene="pyrF"
                     /locus_tag="ON550_000883"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR3953987.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38546..39175
                     /locus_tag="ON550_000884"
     CDS             38546..39175
                     /locus_tag="ON550_000884"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR3953988.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39163..39477
                     /locus_tag="ON550_000885"
     CDS             39163..39477
                     /locus_tag="ON550_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3953989.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39515..40156
                     /locus_tag="ON550_000886"
     CDS             39515..40156
                     /locus_tag="ON550_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3953990.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40199..40891
                     /locus_tag="ON550_000887"
     CDS             40199..40891
                     /locus_tag="ON550_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR3953991.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40888..41790
                     /locus_tag="ON550_000888"
     CDS             40888..41790
                     /locus_tag="ON550_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3953992.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41948..43654)
                     /gene="efbA"
                     /locus_tag="ON550_000889"
     CDS             complement(41948..43654)
                     /gene="efbA"
                     /locus_tag="ON550_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR3953993.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14051 a         7710 c         9503 g        12545 t
ORIGIN      
        1 aataataaac aaaatttttc agctttttta ttcttggaat aacgaggtat tgttttcgat
       61 aaaaaaagat ggtgcagaca aattatgata attaagcata ctatcagata actcaaaatg
      121 aagccttctt tcaaaaaatt attcaaaacc atatattgta ttttttattt gataataaca
      181 atgggattat taagaagtgg cactaaataa aagactagtg tgtatttagc cgatttaatt
      241 ataaacaagt aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt
      301 ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa
      361 tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac
      421 cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg
      481 atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag
      541 gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg
      601 aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt
      661 cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag
      721 tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg
      781 tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc
      841 agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg
      901 ctacgtgtga cgattctgaa gtgacaggtg tcattttttt attgtaacct gtccacacgg
      961 aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa
     1021 taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat
     1081 ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt
     1141 ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt
     1201 cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc
     1261 cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc
     1321 catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga
     1381 tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga
     1441 ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt
     1501 gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa
     1561 tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt
     1621 tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt
     1681 cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa
     1741 gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga
     1801 tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg
     1861 tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat
     1921 cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta
     1981 cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca
     2041 aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag
     2101 gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct
     2161 taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt
     2221 tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca
     2281 tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg
     2341 aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt
     2401 tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc
     2461 gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt
     2521 tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg
     2581 aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc
     2641 agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga
     2701 tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat
     2761 tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt
     2821 catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg
     2881 tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc
     2941 aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa
     3001 tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag
     3061 accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta
     3121 aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga
     3181 tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca
     3241 gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt tttatggata
     3301 accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc
     3361 atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga
     3421 gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt taatcacact
     3481 cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt
     3541 caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg
     3601 atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag
     3661 aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa
     3721 tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt
     3781 ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat
     3841 tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga
     3901 atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat
     3961 tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct
     4021 acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt
     4081 gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat
     4141 gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca
     4201 agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa
     4261 tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag aagaaaacga
     4321 tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt
     4381 gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt
     4441 gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa
     4501 accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga
     4561 aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc
     4621 tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg
     4681 tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag
     4741 gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc
     4801 catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt
     4861 tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct
     4921 ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat
     4981 cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag
     5041 ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg
     5101 ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata
     5161 aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag
     5221 cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga
     5281 gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt
     5341 acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga
     5401 attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt
     5461 tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat
     5521 cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc
     5581 actgacggaa actggcacac attttcagtt ggaagtagct ttgctgaaag accatgtgat
     5641 ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg
     5701 tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa
     5761 agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt
     5821 aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg
     5881 gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga
     5941 tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc
     6001 caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga
     6061 cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct
     6121 aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt
     6181 aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca
     6241 aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta
     6301 ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca
     6361 ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa
     6421 agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat
     6481 gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt
     6541 ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga
     6601 agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga
     6661 acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg
     6721 acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca
     6781 aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta
     6841 tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt
     6901 tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga
     6961 acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt
     7021 gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca
     7081 cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac
     7141 agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca
     7201 agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca
     7261 tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa
     7321 gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa
     7381 attttatcgt tcattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt
     7441 gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg
     7501 ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat
     7561 acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt
     7621 tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt
     7681 gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa
     7741 ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat
     7801 ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta
     7861 ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa
     7921 tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa
     7981 gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat
     8041 cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac
     8101 cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa
     8161 aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag
     8221 gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct
     8281 gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca
     8341 ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt
     8401 tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg
     8461 acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat
     8521 tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt
     8581 tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac
     8641 aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc
     8701 gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg
     8761 agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg
     8821 attcggaatt ggcaggagaa caccgtttat tcggaagaat aggactgtga caagactttt
     8881 gtcacagtcc ttttttcgaa ttcaacaagt aaaactttta agaaatattc agagagtaaa
     8941 attaaaaatt gctgataaac gaagatggaa attttatgta tttttattgg tctatgcaat
     9001 aaataaatta aatattcaga atacaattga ttttaacgaa agggcttcta tcaatggttg
     9061 tgttgtattt atctgcccag gtagagtaaa atagatcatt atctaaaagg taattgttca
     9121 tactatataa gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat
     9181 tctcataatc agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta gatgaatgac
     9241 acgatatatg tttggtatgt tctgcaagac agatgaatat ggaggtggag acaatgtctt
     9301 ccaaagaact aacaatgtat ttagagaaag aattggatca actgaaagaa aaggggttat
     9361 acaatacgat cgacatacta gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa
     9421 agatgatcaa tctagcatcc aacaattatc ttggatttgc caatcgcgaa gaattgaaaa
     9481 aagcatgtat tgaagcaacc gaaacttatg gtgtaggagc aggagcagtc cgaacgatca
     9541 atggttcgct gaaaatccat cagcaattag aagaaaaaat cgcagaattc aaagggacag
     9601 aagcagcaat cgcctttcaa tctggattca actgcaatat gggcgctatc tctgcagtaa
     9661 tgacaaaaga agatgctatc ttatcggatg aattaaacca tgcatctatt attgacggtt
     9721 gccgcttatc cggagcaaaa atcatccgaa ttaaacatca agatatgaaa gaccttgaga
     9781 aaaaagcaaa ggaagcgata gaaagtaaga aatataaaaa aatcatgtat atcacagatg
     9841 gtgttttctc catggatgga gacattgcac gattgccgga aattattccg attgttgaaa
     9901 aatatggact gatcacctac gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg
     9961 caggaaccgt caagcatttt ggattaagcg acaaaatcga tatgcaaatg ggcacgttat
    10021 cgaaagctat aggtgttgtt gggggttatg tagctggatc gaaaacacta atcgactggt
    10081 tgaaagctag aagtcggcca ttcctatttt ctacttcgct aactccaggt gcagctgcag
    10141 ctgcattggc ttcgattact ttgatgcaag aacatccgga acttgtcgaa aaagtttggg
    10201 aaaatgctaa ttattttaaa gaagaattga aaaaagtagg ctataacatc ggtatgtctg
    10261 aaacacctat tactccagtc attttgggag acgaaaaagt gactcagact ttctctaaga
    10321 agctgatcga acatgggatt tatgctaaac caatcgttta tccaactgtg cctcttggga
    10381 caggcaggat cagaaatatg ccgactgcgg aacatacgag ggaaatgctt gatgaagcag
    10441 tagcagttta tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat ttagtttttt
    10501 cgtctattta aaattagaga acatgttcac gcatatattg ttaatcatct gaagatagta
    10561 gtaaaaaaga tcagggagga tcatcatgaa aaaagtattg gtgacaggat gtttaggaca
    10621 aattggttca gagttgatca cacggctacg tcaagaacta ggcagtgaaa atgtcattgc
    10681 gacagatata cgcatgcctg aagtcattga gaaaaacgat atctttgaga tcctagatgt
    10741 aatggattac gaaaaaatgc gcagcatagc agaacaatat caagtggaca cattgattca
    10801 tttggctgct cttttgtcag ctgttgcaga agcaaaacca caattagcct ggaatttgaa
    10861 cgtgactggg ctggtcaatg cattggaagt cgctagagaa ttccagctga aattttttac
    10921 gccaagttca atcggcgcgt ttggtccaaa tactccaaaa gatcataccc cacaagatac
    10981 tcttcagcgt cctacaacca tgtatggggt aacgaaggta gctggagaac tactatgtga
    11041 ttattattat gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg gattgatttc
    11101 ttatgcaacc ttgccaggag gcggtacgac agactacgca gtcgatattt attattcagc
    11161 aatcaaagaa ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg atatgatgta
    11221 tatgccagat gcgatcgatg ccattataaa actcatgaat gcagatcctg atcgattggt
    11281 tcatcgaaat gccttcaata tatcggcgat gtcttttgac ccggaagaga tcaaagcatc
    11341 tattcaaaaa atcataccag attttgaaat ggattatgat gtggatcctg tacgtcagtc
    11401 aatagcagaa tcttggccaa attcactaga tgtttcttgt gcgcaagagg aatggggctt
    11461 ttcgccgaaa tacgacttag atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa
    11521 attggtagag acggtataaa acaagaacca catccaaaaa gttttttata cttttgggat
    11581 tggctatggt atgaaagggg acaagggatg gaagaatatc aagccacgcc agtaaaagaa
    11641 gtaaaaatag aaaatgaatt aaaacgtacg atgggatttt ttactgcttt atcgactgtt
    11701 atgggcacag tgatcggtgc aggggtattt tttaaagccg caagcgtagc agaagttaca
    11761 ggatccgcca gtttgcatat gttttcttgg tttctcggtg gtatgatttc tgtctgtgca
    11821 ggactaacag gagctgaact ggcagctgct attccagaaa caggagggat gattaaatat
    11881 atcgaacgga tttacggaaa tacagcagct tttttattgg gctgggcgca agtcgtgatt
    11941 tattttccag caaatgtagc agccttgtcg atcattttcg gtacacagtt cgtcaatcta
    12001 tttggtcttt cccagtcaat gattgttccc gtagctgtta cagctgctgt atcgatcctg
    12061 ttgatcaatt ttttaggttc taaagctgga ggggcttttc agtcgattac gcttgtctgc
    12121 aagcttattc ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga aggagtagac
    12181 ttccaactgt ttccgatcca agcaggagaa aatctttcat ttttctcagc cttgggagcg
    12241 ggcttgctag caacgatgtt cgcatacgac ggttggatac acgtaggaaa tatttcggga
    12301 gaattgaaaa agccggcgaa agatttgcca aaagcaattt ctttaggaat catcggaatc
    12361 atgatcgttt atttattagt aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc
    12421 gcaggaaaca gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga
    12481 agactggtca cagttggtat cttgatttct gtttatggaa ccatcaatgg ctataccctt
    12541 actggaatgc gtctccctta tgttatggca aaagaaaaca acctgccttt tagcaagctc
    12601 tttgctaaac ttcatgataa aactaaagtt cctgtagccg caggtatctt agagctagtc
    12661 atcgctattg gcatgatgat gatcggtggt tttgatacac taacggatat gctgatcttt
    12721 gtcatttgga ttttttatac gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa
    12781 cctgatttgt ttcgaccata caaagttcca atgtatccgt ttattccact agtagcaatc
    12841 attggaggga cttttatcgt atcaagcacg cttatcactc agaccttttt agcttcgatg
    12901 gggatagtat tgacacttgc tggtatcccg atttatctct atttaaaacg tagatagcta
    12961 aaacattcga attttattag aactgcactt ctctgccaaa caaaatattt tatttaaatg
    13021 aggagtacgt ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt
    13081 tgtctacgat gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt
    13141 tactttgatt tgcgtaactg aactattcgg cgtacgccgc gataaatatt taataaaacc
    13201 aagcaaccag aaatgaaaat gggaagccaa atcagataga cagggtagcc aagcacttta
    13261 gacaaagcat gtggagtcat tttatccatt tcaagaagtg cttgctgcca agtctgaaga
    13321 ttgaagacta gcatgaaacc cgaaataatc aggagaataa atcctaaata atgaattgta
    13381 gttctttttc ttacataagc gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca
    13441 aaaacaaagt acatattcag catctcgaca ccccctttgt gtttatctta ctttactttg
    13501 aaagaaatga gaaataatag cctcgttact tgcttgaaac agcaaaggac tgctaagata
    13561 agagagatac aaagaatcaa ggaggagtca tatggcaagt tatatcgaac cgattcgttt
    13621 tgcgctgatt gtttttccat ttttagcctt agcgatctca tctgtttttt ttatttatga
    13681 atatcgaaag tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt
    13741 ttatttattg tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc
    13801 ccaaatgaca gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga
    13861 caacacggta ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt
    13921 atttttggaa ccagctttta atgttttatt actcatacca tttggcattt atttaagata
    13981 ttatttccgg ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt
    14041 tgaattgacg cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga
    14101 tgtgaatgat ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt
    14161 atttacattt atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa
    14221 gactgtctca ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct
    14281 tattaatttg gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc
    14341 tcatatctgg tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac
    14401 caatggatgg acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga
    14461 acgaatttct ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg
    14521 tttaagctgg ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt
    14581 gcagatgagt gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt
    14641 aaatatcttg ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca
    14701 cacatatact gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag
    14761 caaaaacgct ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc
    14821 acgaatgaat aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc
    14881 aaagggcttc aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg
    14941 aaaaaatatc attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc
    15001 tttgtttgaa aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag
    15061 tagggaacta gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc
    15121 ggagatccgt gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct
    15181 gcatcaccaa cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa
    15241 tgaacaagcg ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac
    15301 attggatatg ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct
    15361 gcagttttta gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata
    15421 catggaaaat ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga
    15481 aaattggctg atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac
    15541 aattggtcat ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat
    15601 tgccgattac ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc
    15661 aagagagaga aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc
    15721 gactgattta gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga
    15781 aattccagaa gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt
    15841 aaaaggaacg gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga
    15901 aaaattgggc gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata
    15961 cgatcgtaat agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat
    16021 tggtcttgtg aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg
    16081 ggcaattgca gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac
    16141 tgctcgtaaa aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg
    16201 tgtcacaatc tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc
    16261 ttcaataaaa ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt
    16321 catttcagct actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc
    16381 ttcaagcaac tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc
    16441 cggcttgaag caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg
    16501 atcaagaaat agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc
    16561 aggaatccct tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat
    16621 ccattcatcc gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga
    16681 tgtattgatt cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg
    16741 aaaataaaaa ttgagatatt gacagtgatc aatagaatgc gtatactaat taagtacgaa
    16801 aaggatatga tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata
    16861 gtcactaatc aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt
    16921 tatcgagttt aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt
    16981 cgtccttggc tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg
    17041 aaacaattaa gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga
    17101 catacgattg aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc
    17161 aactctggtg ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca
    17221 agaatcacaa atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca
    17281 gcacggcacc atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa
    17341 aatgaagcca tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat
    17401 ccagaaaaac tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat
    17461 gaagaagttg gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc
    17521 ggtgcaggcc catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac
    17581 gatttagcag aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa
    17641 atctggaact tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt
    17701 cctcataaaa acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat
    17761 gcgtcaacga actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta
    17821 agcaaacaag taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca
    17881 gatcatattc gcgccctttc atttgctatt ggcgacggtg cattgccatc gaatgaaggg
    17941 agagggtatg tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt
    18001 attgaacaag cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat
    18061 tatcctgaag tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa
    18121 cgtttcaatg aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta
    18181 aaaaacgaaa acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat
    18241 ggcttcccgg tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat
    18301 gctggttttg aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact
    18361 gaaaaatcaa tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc
    18421 attggataca cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct
    18481 cttttgaatg aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat
    18541 gcagaaatgg gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc
    18601 gtagcagttg ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa
    18661 gtaaaagcac caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc
    18721 aatcggatca tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta
    18781 cttggcgagc atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac
    18841 tttacccact ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac
    18901 gaaaaaatct gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa
    18961 aacatgggtg caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat
    19021 attggcggct ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc
    19081 ggaatcttca aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct
    19141 gtaacaagta aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta
    19201 gcaacaattg ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa
    19261 cagcaagtaa gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa
    19321 caagctggtg atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca
    19381 caagtaaaag tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa
    19441 aatatttcag atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg
    19501 gcaatgacga aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc
    19561 gcaccgaaag taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa
    19621 aatcctgctg gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag
    19681 ggcgtttacg tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg
    19741 gcgaagacaa acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa
    19801 tggtcggatt cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata
    19861 aataaaaagg ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc
    19921 gatcttgtta gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt
    19981 aggattcatt agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg
    20041 cccaaaagat ggcatggtga ttggcttgtt ttggggaatc gctacaattg ttcgtgccta
    20101 tgccattgca acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt
    20161 tcccagagta cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa
    20221 taaatcaata acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt
    20281 cttagtgtta ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt
    20341 agactctgct cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga
    20401 agcaataggt gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc
    20461 gttgaaacca gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa
    20521 ggaagagagg agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac
    20581 tagaaggggt aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac
    20641 agcggacaaa aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt
    20701 ttgtctctaa gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag
    20761 tgaacagtct atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg
    20821 caaagccagc agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt
    20881 tatttgtttc gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa
    20941 aaatcatcca tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt
    21001 ccatcttttt gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt
    21061 ctgaaacatt gagagctaca gcaacagata gttataatga tgtctttact acactggctg
    21121 tattgctttc agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt
    21181 ttgctttagc attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg
    21241 aactattagg aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg
    21301 cttgcgatga tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac
    21361 gctttgcatc agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga
    21421 ttattgatct gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact
    21481 tggatccaat gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa
    21541 ttcttcttga aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa
    21601 aaacgctgca atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag
    21661 aaagaatctt acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt
    21721 ttgatcacaa ttatctttta tattaaatga gaaactataa aaagactcct taaagttttt
    21781 aatttaaaga gtcttttttt cttttaattg tttatggaga gcctgacgtg caagattatc
    21841 tgctccttta tttttgtttt cgggcaacca ttgcaggatc aataaagaaa attcggatag
    21901 aagttcttgg attctttgaa ggtagggaag gaagagctcg ttcttcgttg cgtttttgtc
    21961 gaccgtagaa actagtgttt tgctgtctga aaaacagaaa atcgtttcat tctgtaagtc
    22021 gtttttcttt agaaattcta aagtttttaa aaagacttcg aactctgctt gatggtttgt
    22081 ccctatagaa agcgggatag atagctgttc ttgtttacct tgatagacaa tcaagattcc
    22141 acctccacta ggaccagggt tgccttttgt agaggcatca atgtatactt ttaacataaa
    22201 aagagattct cctttttgtt caaactaatg ttatggtaag attatatcat tactgaatcg
    22261 gtgagaggtt agagaatgaa aaaaaatatt cgatggcagc ctgaactggc acatacgatc
    22321 atctattggt cattgacctt gatcattctg ttttttagtc tgattctttc tttagaaaac
    22381 acaggtcctt attggaaaag caattttgtt ttaggtattt ttcttgtttt tctatttcta
    22441 ggtttgcggc gttcttttat tttaaaatca aactctgtga ttatacgtta tgctgctttt
    22501 tggaaagacc gagaaatatc tgtcggacag attcgatctt tgacgccaac taatactggg
    22561 gtgcaaatga tgttgcaaga tgagaaagcc ccatctgttt tcatgatgcg aaaaaaagat
    22621 caagtagtcc tttttgatca ttttgaaaaa aacggaccca aatcagaatc aattccttat
    22681 ccttatacga aaaaaagaaa aagctaattt taaaaatcag aacctaaaaa aacgaatttt
    22741 tacaggaaaa gaaaagagtg agacgtacgg ttcatcccgt ttagcgtctc actctatttg
    22801 tttatctatt catgcatact agtatcggta gtctgaacgt tgcctttctt gacatgggca
    22861 gcttgtgttc ctcgattccc ttcgatgatc tcaaattcga ccacttgatt ttcttccaaa
    22921 gttttgaatc cctcttcttc aatcgcagta aaatgtacaa aaatttcttc tgtgtcatca
    22981 taactgataa agccgtatcc ttttttgttg tcaaaccatt ttacaattcc tgtggtcaaa
    23041 gcactcacga cctttctaaa gtctgatcat agccttcatt ataaagagta tttataagta
    23101 cgtcaaataa attaaaaacc tgtaatttta tgtttttttt ctgaataaaa aaaggtataa
    23161 ttggcatatc ttaacaaaga agatattttg ttgaataaag ttcgtgaaaa ggggtaatca
    23221 aatgaagaat gatctgcaaa tttcacaaga aacagagcta gaaccgattg ttgaagtggc
    23281 tagtaaatta gatcttaagg aagacgattt ggaactgtat gggaaatata aggcgaaaat
    23341 caatttttct ggaatcaatc gatcaaagga taacgctgag gggcatttga tcttagttac
    23401 ttccatcaat ccaacaccag ctggcgaagg aaagtcaacg atcactgtag ggttaggaga
    23461 cgcgctcaat aaaatcaata aaaaatctgt tattgcattg cgagaaccct cacttggtcc
    23521 tgtgatggga atcaaaggtg gtgctgcagg cggaggatat gcacaggtat tgcctatgga
    23581 agatatcaac ttgcatttta ctggtgacat gcatgctatc acaacagcta ataatgcatt
    23641 atctgctctt ttagataatc atattcatca aggaaatgaa ttgaacatcg atgcccgccg
    23701 agtgatttgg aaacgggtag tcgatctcaa tgatcgtgaa ctgcgtaaag taattgttgg
    23761 cttgggtggg ccaatccaag gtgttccaag agaagatggg tttgatatca cagttgccag
    23821 cgaaatcatg gctatcttgt gtcttgcggc agaccttgaa gatttaaagg ctcgtcttgc
    23881 aaggatcgtt gtcggctata catttgaccg tcagcctgtg actgctggag acttgaaagc
    23941 agaaggcgct ctggcattac tgctgaaaga tgcaatcaag ccgaatcttg tacaaacaat
    24001 ttacggaaca ccagcttttg tccacggggg accgtttgcc aatatcgctc atggctgcaa
    24061 tagtatttta gctaccaaga cagcattgag attagcagat tacgttgtca cagaggcagg
    24121 atttggtgca gatcttggcg gagagaaatt tttagatatc aaagtaccta acttgaaaaa
    24181 agcgccggat gctgtggtga ttgtcgccac tgtccgtgca ttgaaaatgc atggcggaat
    24241 gaaaaaagat gaacttaaaa atgagaatct tgatgctttg aaagtaggtt ttgcaaattt
    24301 aaagcgccat atacgtaata tggaacaata tcagctacct gtgatcgtcg caatcaatga
    24361 attcgtaaca gatacagaca aagaactcac actgttggag cacttgtgtg aaaatcaggg
    24421 gattcttgcc aagagagcga gtgtatgggc agatggagca gaaggcggcg ttgatttagc
    24481 aaaagctgta gtacgattga ttgatagaaa agaagctaat tacaagccat tataccgcct
    24541 agaagaaacg atacaagaaa agacagaaac aatcgtcaag aaaatttacg gaggaaatgg
    24601 tgttgttttc agcaaaaaag cgcaaaaaca aatagaagag tttacgaaga atggatggga
    24661 caaactgccg atttgtatgg caaaaacaca gtattcattt tctgatgatc cttcactgct
    24721 aggtgcaccg gaagatttca ccatcacgat ccgcgaaatc atcccaaaat taggggcagg
    24781 atttttagta gccttgactg gagatgttat gacgatgcct ggtttgccta aaaaacctgc
    24841 tgcattgaat atggacgtga caaatgacgg agaagtgttg ggattgtttt aagcttgatt
    24901 ataatctacc atcgaaggag ctgtattttg actacagctt ctttttttag acgataggga
    24961 attaattttg tttacactag gatcattctt tttctttatt gtattttgag ctatactaaa
    25021 gcataatagg agggtatgat gaaaacgaca tttataagaa aaaccggttt ttacggtatg
    25081 ggtagctcga ttcagctaag aatcaacaat gaaaaacgca gtgttctcag tcatgaacaa
    25141 aagataacgc ttgatctgga agtgcccttt acgatgcagg tcacctttta ttggctaaaa
    25201 agccccgttt atacagtgaa agaagcaaag cctgcttatg tgatcacaat gaattacttg
    25261 attcttcaaa tctatccgtt tttatttcta gcaactgggt tgggggccgt tttcctacaa
    25321 aatttatttt tcagtttttt tgcagttatt gtgttgattg cgttcttttt ctatataaaa
    25381 aaccgggcat atttgatcaa ggaggaaaat tatggcaaac tctgatcttt ttttaatcag
    25441 tttcaatcga atcgaaaaat ggatgagaga cgagatggga aatgcacgaa atatgggatt
    25501 taccgaactt gtcagacgcc tagctcaaag aaaacaatta atgatcagaa aatacgaaga
    25561 tgatctttta cagcttgctc agttaaggaa tgcgatcgtc catgatcgga tcgcagtcga
    25621 ttttgtcatt gcagagccaa atgattggac agtcaagcga atccagctga tcgaacaaga
    25681 actgattcag cctgaaaccg tgctgcctcg atttgcaaaa cgtgtaacag gttttgaaca
    25741 agatcttccg ttacttgaac tgttaaagat agtagcagaa aaaaggtatt cacaatttcc
    25801 gttatacaat aaaggaaaat ttgtagcatt gattacttta cgtaatatcg ggttttggct
    25861 agcaaaagaa agccaaaaag gtccggttga tttaacaaat aaacgagcgt tggatttgat
    25921 catccaaaac ggaaagtaca caaattatca ttttgttcca gctaaaacac atatttacga
    25981 ggttgaagcg atgtttcgtg agcaagggac tttggaagcc gtacttatca ctaaagatgg
    26041 gaatcccgat ggtaatctgc tcgggatcgt acgacctcgt gatatttata aacaaataga
    26101 aaaggattga ttttttgtta attatttatt ggatattcag cgctttgcta gtaggagtgg
    26161 atcagttggt caaatggtgg atcactgata atctagcgct tggagagacg aaaaatctta
    26221 taccaggtat tttgtcactg aatcatattc gaaacactgg agccgcatgg agtatgctag
    26281 aagggaagat gtggttcttt actatagtga cactcattgc agtcgttgtg attctcacat
    26341 tgatgattaa aaatcgtgaa aaaggtaatc gctggttcat gattggattg agtttgattc
    26401 tagctggtgc gattggcaat tttatcgacc gtattcgtct tggctatgta gtagatatgt
    26461 tccagacaga ttttatgagc tttccgattt ttaacgtagc agatgtgaca cttgtatgtg
    26521 gagtgatttg tatgttaatt tatattattt tagatgaaaa ggatcaaaga aaaaaatgac
    26581 agaacttaaa acgacgattc accatgaatc tggtaggatc gataaagtat tgactaattt
    26641 attcaccgat tacagtcgtt cgcaaattca gctgtggctg aaaaatggag cggtttctgt
    26701 caatggacag ccagtcaaag caaattacaa agtaaaacaa ggagacgaga ttactgtctt
    26761 gatccccgaa ccagaaacat tagatgtggt cccagaaaat atcccgttga cgatcgttta
    26821 cgaagatgaa gatgtagcgg tgataaacaa acctcaagga atggtcgttc atccttcagc
    26881 aggtcatacg catggcacaa tggtcaatgc gctgatgtac catatcaagg atctttctac
    26941 gatcaacggc gttattcgtc caggtattgt tcaccgaatc gacaaagata cttccggact
    27001 gctaatggtt gccaaaaatg accaggcaca cgaatcatta gctcagcaat taaaagataa
    27061 gacctctttg cgcaaatatg ttgctttagt ccatggagag attcctcacg aaaaaggaac
    27121 catcaatgcg ccaatcggcc gttcgaagga aaaccggaaa atgcaggcga taagagaaga
    27181 tgggaagtcg gctgtcacac attttactgt attggaacgt ttcgaaggat ttactctggt
    27241 tgaattaaaa ctagaaaccg gaagaacaca ccagatccgt gtgcatatga agtatatcgg
    27301 ttatccgtta gctggagatc cagtatacgg accgaagaaa actttgaaag gcaatggtca
    27361 gtttctccat gcgcaaatgt taggttttac gcacccgact acaaacgaaa agatgatctt
    27421 tgaagcacct cttccagaag tttttgaaaa aactttagaa aaattaagaa aaaacattgc
    27481 attttaattt agataacggt atagtaatta acaacgaaag cataaaactt aatagaattc
    27541 ctttaagttt agtcccgtga ggctaagaag ggtgggagca aggatacgta tggtgcgcca
    27601 taagtagatt gctcattgac atagtatagc tatcagtgta tcagtatacg ctcagtctaa
    27661 acattcctcc tgtcctctta gggcaggagt ttttttatgc aaaaaagtag ggggaatgaa
    27721 aatgcaagca aaagaagtag tagatcaggt aaccatgaag cgagcattga ctcgaattac
    27781 ttatgagatt atcgagagaa accatagtat ccaagatatc gtattggtag gtatcaaaac
    27841 acgaggaatc tacattgcat caagaattgc cgaacgcctg aagcagctag aagatatcga
    27901 tattcctgta ggagaactgg atatcacttt ataccgagat gacaaaaaag aaaatccaga
    27961 agaaccggaa cttcattctt ctgacatccc agtttcctta gaaggaaaag aagttatttt
    28021 gattgatgat gttttgtata caggacgaac gattcgagca gcaatggacg cagtaatgga
    28081 ttttggcaga ccaagaaaaa tttcattagc agtcctggtc gatcgtgggc atcgagaatt
    28141 gccaatccgt gcagattatg ttgggaagaa cattccaaca tctcgggcgg aagagatttt
    28201 agtagagatg caagagcttg atggacaaga tcgcatcatg attttgaaag aggaagatta
    28261 gaggagagag aaacatgtcg gagaaaaaag gatttcataa tgatgacgtg gtgttagata
    28321 tccacgatcg cccaacccca ggacattggg taggattaag tttacaacac ttgttcacga
    28381 tgttcggagc aaccgtgtta gtccccatct tagtaggtat cgatccaggt attgcgctag
    28441 tcagttcagg actaggtaca ttggtgtact tatttactac taaaggaaag atacctgcgt
    28501 acttaggaag cagcttcgct ttcattgcag cgatgcagct tttgatgaaa acagatggtt
    28561 atccagcgat tgcacaagga gcagtcacaa ctggattagt ctatttgatt gttgcgctga
    28621 tcatcaaaaa gatcggttca gcatggctgg ataaaatctt gcctccaatc gtcgtcggtc
    28681 cagtcatcat ggttatcggg ttgggacttg cggcaaatgc agcaacaaat gctatgtata
    28741 atcagggcac gtatgatttc aagtatattg ctgtagcgct gatcacatta ggattaacaa
    28801 ttttcttcaa catgtggcta agaggttttc tcggattgat tccgatttta ctaggtatta
    28861 ttagcggata catcgtagcc ttgctgtttg gaatcgtgga tactaagcca atcgtagacg
    28921 ctgcctggtt tgctgttcct aactttgaga ttccctttgt acaatatgaa ccaaagctat
    28981 atcttggagc aatcacaaca atggcaccca tcgcttttgt gacgatgaca gagcatatcg
    29041 gccatcttat ggtattgaat aaactaacta aacgtaattt ctttgaagaa ccgggtcttc
    29101 ataagacgtt gacaggtgat ggattagcac aaatcgtggc agcctttgtc ggaggtcctc
    29161 ctgtaacaag ttacggtgaa aacattggtg ttctggctat cacacgtgtc catagcgtgt
    29221 ttgtgatcgg tggagctgca gtatttgcag tcattttagg attcgtagga aaattaagcg
    29281 ctttgatttt aagtatccct ggaccagtca tctcaggaat cagttttatc ttatttggag
    29341 taattgccgc aagtggaatg aagattttaa tcgagaacaa aatcgatttt gataaaaaga
    29401 aaaatctgtt gattgcttca gttatcttag tagtaggaat tggcggactc gtctttgaag
    29461 taggcacatt tacattgtcg gcaatggcat tagcaactgt acttggcatc attttaaatc
    29521 ttatcttacc agaaacttca cgaagcgaag aaaactaagg aggaaacatt atgatcatca
    29581 aatcagaacg tatcagctta aagcatttat tgaccgttga ggcacttact gatcaagaag
    29641 ttatgggctt gatccgcaga gggcaggaat tcaagcaagg tgctaattgg actccgcaaa
    29701 agcgccagta ctttgctaca aacctctttt tcgaaaatag tactcgcaca cataaaagct
    29761 tcgatgtagc ggagaaaaaa ctagggctag aagtgattga atttgaagca agcacaagct
    29821 ctgtccaaaa aggggaaaca ctatacgata cagtactgac gatgtcagca ttaggcgtag
    29881 acgtagcggt tatccgtcat ggtgatgaga actattatga cgaactgata caaagtaaaa
    29941 caatccagtg ttcgattatc aatggaggag acggaagcgg acagcatcca acccaatgct
    30001 tgcttgattt gatgaccatt tatgaagaat ttggtcattt tgaaggtctg aaagttgcca
    30061 tcgtaggtga tatcactcac tctcgcgtag ctaaatccaa tatgcagatg ttaaaacgtc
    30121 taggcgcaca agtattcttc tcaggtccta gagaatggta cgacgatgaa ttcgaagtgt
    30181 acggacatta tatgccattg gatgaactac tagatcaagt tgatgtcatg atgatgcttc
    30241 gggtgcaaca cgaacgccat gatggaaaag aaagtttctc aaaagaaggc taccatcagg
    30301 aacatgggtt aacagttgag cgtgcgaaga agttgcaaaa acatgcaatc attatgcacc
    30361 cagcaccagt taaccgtgat gtagaattag ctgattcatt agtggaagga ttacagtcgc
    30421 ggatcgttca gcaaatgagc aatggagtat tcgtcagaat ggcaatctta gaagcagtgc
    30481 taaatggaaa agcgtaatta taggaggatg aaaaaatgaa aacactcatt aaaaacggtc
    30541 agatcaatac acgaaaaaat atgacgacac cagcagaaat ttggatagaa gatgggcgaa
    30601 tcaaagcaat cggtacagga ttctcagaag ccgaatttga tgaagtgttt gatgcaaagg
    30661 gacaattgat cacacctggt ttagtggacg tccatgttca tttgagagag ccaggattta
    30721 catacaagga gacgatcgaa gctgggactc gctcagctgc tcgtggcggt ttcactactg
    30781 tatgcgcgat gccgaattta aatcctgtac ctgatacggc agaaaagctg agacaagtat
    30841 atgacatcat tagaaaagat gcagttgtga aggtattgca gtatgcgcca atcacagaaa
    30901 atctgcgaag tgagaaatta gttgatcaag aagcactgat tgaagaaggt gcttttgctt
    30961 tcacaaatga cggtgtaggt gtccaaacag ctgggacgat gtatctggct atgaaagaag
    31021 ctgcaaaaaa caataaagcc ctagtagccc atacagaaga tgaatcttta ttatttggcg
    31081 gcgttatgca cgctggcaag aaggcagaag aacttggtct tcccgggatc ttaagcgtaa
    31141 ccgagtcttc tcaaattgct agagatctgt tgctagctga agcaactggt gttcattacc
    31201 atgtttgcca tgtttctact aaagaaagtg tccgtgtaat cagagacgca aaaaaagcag
    31261 gaatccacgt aacagcagaa gtttcacccc atcatcttat tttgatcgac gaagatattc
    31321 ccgaagattt cggcttttgg aaaatgaacc cgccgctaag aggacgggag gacagagaag
    31381 ctttgatcga aggcttgctt gacggtacga tcgattgcat cgctacagac catgcccccc
    31441 atggattaga agaaaaaagc caaagtttca tgaaatcacc atttggtatc gtaggaagcg
    31501 aaacagcgtt tcaactgatc tatactcatt ttgtagaaac tggtcgattc acgttggaac
    31561 aagtgatcaa ttggttggca gttaaaccgg ctgagatttt tggtttgaat gcaggaacgc
    31621 taacggttgg tgcccccgct gatgtagcag tttttgatat tacacaaact tgcacgatcg
    31681 ataaagaaga ctttttatca aaaggggaga atacaccttt tatcggctgg aaagtaaaag
    31741 gcgaaacaca aatgactttt gtcaacggaa aacttgtgtg gcagaaggga gaataaaagt
    31801 atggaacgtt tgcttatatt agaagatgga acagttttcg aaggaaaggc ttttggtgca
    31861 gatgctagtg tcttcggaga aatcgttttt acaacaagta tgactggtta tcaagaaact
    31921 atcactgatc aaagttttaa cggtcaaatc attacattta cctatcctat ggtgggtaat
    31981 tatggggtaa atagagatga ttatgaatcg atttctccaa catgcaaagg agtagtagta
    32041 aaagaacatg cacgtgtagc ttcaaactgg cgaaaccaaa tgacgctaga ttcatttttg
    32101 aaacaaaaag gaattcctgg aatatcaggt atcgatacgc gcgcccttac tcgcaaaatc
    32161 cgtgaagtcg gaacgatgaa agctagtata gttgatgctg gagattcctt cgaacatgca
    32221 tttgatcagc tgaaagcaac cgttctagca accaatcaag tgcagcaagt ctcaactaac
    32281 aaaccttatc caagtccagg aacaggtcgg aaagtcattg tcattgactt cggcttgaaa
    32341 catagtattt tacgagaatt gagtaaaaga aactgtaacc tcacagtact tccctataac
    32401 acagatgccg agacgatttt agcgctatca ccagacggag tcatgctgac gaatggacct
    32461 ggtgatccaa aagatgtgcc agaagcaatc gaaatgatca aaaaaatcca aggaaaagta
    32521 ccgatttttg gtatttgtct cggtcaccag ttatttgctt tagccaacgg tgcagatacc
    32581 tataaaatga aattcggaca tcgaggatta aatcacccgg tacgagaagt agcgaccgga
    32641 agaattgatt ttacttctca aaatcatggc tatgcagtcg atgaagccac gattgatcca
    32701 gaaaaactca tcgtaactca tgtagaagta aatgacggaa cagtagaagg agtcaaacac
    32761 agagattatc ccgctttcag tgtgcagttt catccagatg ctgcccctgg tccgcatgat
    32821 gcactacatt tatttgatga atttatggaa atgatggacg caggaaagga gcagtaaaca
    32881 gatgccgaaa cgtacagata tcaagaaaat tatggtgatt ggatctggtc cgatcatcat
    32941 tggtcaagcc gcagaatttg actacgcagg aacacaagcc tgccttgcct taaaagaaga
    33001 aggatatgaa gtggttttag taaattctaa tcctgcgacg atcatgacgg ataaagaagt
    33061 ggcagatcaa gtatatatcg aaccgatcac acttgaattt gtatcaagga ttcttcgaaa
    33121 agagcgtcca gatgctctcc ttccaacttt aggcggacag acaggcctca atatggcaat
    33181 ggagctggca gcatcaggta ttttagaaga attgaacata gaacttctag gaacaaagct
    33241 ttctgcgatc gatcaagcag aagatcgtga tctattcaaa cagttgatga aagaattgaa
    33301 tcagccaatc cccgagtctg aaatcgtcaa tacagtagaa gaagctgtag attttgcaaa
    33361 taaaatcggc tatcctgtca tcgttcgtcc agcctttact ttaggcggaa ctggtggagg
    33421 tatgtgtgaa aatgaagaag aattacgtat cgtatcagaa aatggattaa gtctttcccc
    33481 cgctacccaa tgtttgattg aacgaagtat tgcaggtttt aaagaaattg aatatgaagt
    33541 gatgagagat tcggctgata atgcaatcgt tgtctgcaat atggaaaatt ttgacccggt
    33601 gggtatccat acaggtgatt ctatcgtatt tgcccctagt caaacattgt ctgaccatga
    33661 atatcaaatg cttcgtgatg cttctctttc gattattcga gcacttaaga tcgagggagg
    33721 ctgtaatgtc caactcgctc ttgatccaca tagttttaat tattatgtga ttgaagtcaa
    33781 tccgcgtgtt tctcgttcat ctgcgttagc aagtaaggcg actggctatc cgatcgctaa
    33841 gctagctgct aaaatagcgc ttggattaac attagacgaa atgaaaaatc ctgttacagg
    33901 aacgactttt gcagaatttg aacctgcatt agattatgtt gtagctaaaa ttcctcgctg
    33961 gccgtttgat aagtttgaaa acggagaacg gcttttaggc acgcagatga aagcaactgg
    34021 tgaagtgatg gctatcggtc ggaatatcga agagtcttta ttgaaagctg tgcgttcttt
    34081 agaaatcggt acacaccata tcgaacttcc tgagttggca gacatcacgg acgatgacct
    34141 aatccaaaaa gtggttcgag cacaagatga tcgcctgttt tatgtagctg aagctattag
    34201 aagagggtac accatagaag aattagcgga tttgacaaaa atcgatttat tctttttaga
    34261 taaactgttg catatcgtag aaatcgaaca gcagttgaaa cagcagccgt tggatgacaa
    34321 gatattgaaa acagcgaaac aaaacggttt tacagacaaa aaagtggcgg agctgtggga
    34381 aataaacgaa gaagccgttc gccagatgag aaaagaaaat gatttgattc ctgtatataa
    34441 gatggttgac acttgtgcag cggaattcga atcaaaaaca ccttattttt atagttccta
    34501 tgaatatgaa aatgaaagtt cacgatcaga aaagccctca attctagtac tgggatctgg
    34561 tcctatccgt atcggacaag gggttgaatt tgactatgcg actgtgcatt ctgtcaaagc
    34621 aatccagcag gcaggatacg aagcaatcat catgaacagc aatcctgaaa cagtttcgac
    34681 tgacttttct atttcggata aattatattt tgagccattg accttcgaag atgtgatgaa
    34741 tgtgatcgag ctagaacagc cgataggagt aatcgttcaa tttggtggtc agacagcaat
    34801 caacttagcc gaacctttga ctgctgctgg tgtaaaaatc ttagggacaa cgatcgaaga
    34861 cttggatcgt gcggaaaatc gtgatttatt cgagcaagcg ctgaaagagt tggatattcc
    34921 tcaaccttta ggggatactg ctacaagtaa agaagaagcc gtggttatcg cttcgagaat
    34981 tggttatcca gttcttgttc gtccaagcta tgtattaggc ggacgtgcga tggaaatcgt
    35041 tgaaaaccag cgagatttag aagattacat ggaacatgct gtgaaagcat ctccagaaca
    35101 tccagtacta gtggaccgtt acttgcttgg tggtgaatgc gaagtggatg cgatttgtga
    35161 cggtgaaaca gtactcatcc cagggatcat ggagcatatt gagcgtgctg gtgtccattc
    35221 aggggattca atggctgtgt atccacctca aacattatct gaatcgatca agaaaacgat
    35281 cgaagattac acgatccgct tagcattagg tttgaattgt gtcgggatga tgaatatcca
    35341 gtttgtcatc catgaaaatg aggtttatgt gatcgaagtc aatcctcgag caagtcggac
    35401 tgttccattc ttaagtaagg tgacaggaat tccaatggca caaattgcga cgaaagctat
    35461 tttgggagag aaactaaaga cacttggtta tgaaaacgga ctttatccag aaacaaaaga
    35521 agttcatgtt aaagcacctg tcttttcttt tacgaaattg cagaaagttg atacttactt
    35581 agggcctgaa atgaaatcga ctggagaagt aatgggatct gacagacaat tagacaaagc
    35641 tttgtacaag gcattcgaag cttctggatt acacttgcct gaatatggag ccgtattatt
    35701 tacgattgcg gacgaaacaa aagaagaagc actagaatta gctaaacgtt ttacagaaat
    35761 tggctatagt ctgataggaa cgaaacatac agctgcttat ttcgaaaaaa atggtctggt
    35821 cgttgaaaca gtagcgaaaa tttctgagga agcagcagaa aaaaatgtag tcgacttaat
    35881 aagagatggt aaaacacaag tggttgtcaa tacgattgat aaagatcggg gaaatgcttc
    35941 taaagacggt ttcatcatcc gaagagaagc agttgaacat ggtattccat tgtttacttc
    36001 tttagacaca gcagatgcga tcgttcgagt gatggaatcc cgtgcttttt caactaaccc
    36061 tatttaacgg cgagcacatg ttaagacaaa aagacgaaaa accgttgcag gaggggacag
    36121 atgaaacaag aaatgatgga aatcgttcag caaaaacagc tggcaccaaa gatttttgaa
    36181 atgacattga aaggtgatct agtaaaagaa atgcagcaac caggacagtt cttgcatatt
    36241 ctcgtgccgc gtgcagactt gctattacgc cgaccgatta gcttgaatca tatcgaccac
    36301 gaaacaaata cttgccgtat catttatcgg gtggaaggtg aggggactag agtcttctct
    36361 actctttctg ccggagacca tttagatgtc atgggtcctt taggcaatgg ttttgatttg
    36421 tccaatcttc atacaggaga cgaagtattt ataatcggtg gaggaatcgg tgtccctcct
    36481 ctatatgagt tatcaaaaca gttagtcgaa aaaggaatta agccaacgca ttttttaggt
    36541 tttgccacac atgaagtcgt ttattatgaa aaagaattta atcgtctagg agaaacaaga
    36601 atcgctactg acgatggaac atacggtatg cacggaaatg tcggtaattt attgttagga
    36661 acgaagacgg aacctacagc tgtttttgca tgtggttcaa acgggttatt gaagacagtg
    36721 gaacagctgt tttcttcgca tcaaaatgtc cagttatcat tagaatcccg catggcatgt
    36781 gggatagggg cttgctatgc ttgcgtttgt catacgccgg aagatgaaac aggaacaaaa
    36841 agtgtgaaag tttgtgacga aggaccgatt tttaaaatag gagaggtaat tatatgaatg
    36901 agaatccttt agcggtcaaa cttcctggcc tagaattaaa aaatcctatc atgccggcaa
    36961 gcggctgttt cggttttggc aaagaatacg gaaaatacta tgatttgaat cagcttggat
    37021 cgatcatggt caaggcaacg actccaaatg cccgctttgg caatcccacg ccaagagtgg
    37081 cggaaacacc atctggcatg ttgaatgcaa ttggtttgca aaatcctggt ctagacgttg
    37141 ttatgcataa acttcttcca gaattagagg agtacaatga gttacccatc atcgcaaatg
    37201 ttgctggtgc ttgtgaagaa gactatgtag aagtttgtag caaaattggc gaagcaccaa
    37261 atgtgaaagc aatcgagctg aacatttctt gcccaaatgt caaacatggc ggaattgctt
    37321 ttggaacaga ttctgatgtt gcttttcaat taacacaagc agtaaagaaa gtttcttctg
    37381 ttcctgtcta tgtcaaattg tccccaaatg taacagacat cgttccaatc gctcaggcaa
    37441 tcgaagctgg aggggcagat gggtttacga tgatcaatac tcttcttggt atgcggattg
    37501 accttaagac aagaaaaccg attttagcca atcaaacagg tggactctcc ggaccagcaa
    37561 tcaagcctgt cgctattcgt ttgattcacc aagtagcaag tatatccagc cttccaatca
    37621 ttgggatggg cggcgtgcag acagtggatg atgttcttga gatgtttatg gctggagcaa
    37681 gtgctgtagc tatagggacc gcgaatttca ccgatccgta tatctgtcct aaactaatta
    37741 aagagctccc tgtgcgaatg agcgaactag gcatcgaatc gctggaaaga ttaatcaaag
    37801 aagtgagaga ggaacgtgaa cgatgaatca actagaccaa cgaccaatta ttgcattaga
    37861 tttttcaact aggcaagagg tagaagactt tcttcgattt ttcccgaaag aagaaaaatt
    37921 gtttgtgaaa atcggaatgg aattgttcta ccaagaagga cctgaaatcg tacgttatct
    37981 aaaagaggct ggtcataatg tatttttaga tctgaaactt cacgatattc cgaatacggt
    38041 cgaaaaagca atgcggggat tggcaaagct tggtgttgat ctcacctgtg ttcatgccgc
    38101 tggcggtatt cgtatgatgg aggcggcaat gcgaggatta gaagagggga ctccagaagg
    38161 tggaaaacgt ccgctacttc tagctatcac tcagcttact tctacaagtg aagaagaaat
    38221 gcatgccgat caactaatcg aagtttcttt agaaaaaagt gtgatccatt atgctagctg
    38281 tgccaaaaaa gcaggattag atggtgtggt ttcttctgct tgggaagtag aagcgatcaa
    38341 agaaatggca ggagacgaat ttgtttgctt aactccgggt ataagaccag aaggaacagt
    38401 cgcaggagat cagacaagag tagtcacacc ttctcaggca aaaaagatcg gctcaacttt
    38461 tatcgttata ggtcgaccaa tcactcagtc aactaatcct tataaagcat atcaaacgat
    38521 tcaaacagaa tggagctaac ctaaaatgaa tgttgaacaa agtatcgcaa aagacttatt
    38581 agaaattgaa gcagtatttt taaatccaag taatcctttt acatgggcaa gtggaatcaa
    38641 aagcccaatt tactgtgata accgaatcac gatgagctat ccaaaagtcc gaaaagagat
    38701 cgcaaaagga ttagcaagca aaatcaaaga agcttaccct gaagtacaag tgattgctgg
    38761 aactgcaaca gcaggaatcc ctcatgccgc atgggtagcg gaaatactgg atttgccaat
    38821 ggtctatatt cgaagtaagg cgaaagatca tgggaaaggt aaccaaatcg aaggacgtat
    38881 atttgaaggt cagaagatgg ttgtgatcga ggatttgatt tctacaggcg gaagtgtctt
    38941 ggaagcagct gaagcagcaa aaagagaagg tgcggatatc cttggtgttg cggctatctt
    39001 tacgtatgag ttgccaaaag gaaaagcaaa tttcgaaaaa gccgaaatcc ctcttatgac
    39061 gttgaccaat tattctgtat tgattgaagc agctttagaa gatcgttata tcgatgagca
    39121 agaattaact ctgctaaaag aatggaaaaa agatcctgaa aattggcaga tttaagcgcg
    39181 cttgatgaag atagagaaat ccgatttgtg tctccagaat catcaattgg aatagcaaaa
    39241 atccaaaaac tgattgcgag aagcatcttt tcatcaggta tgaaaagaaa cttctatcgt
    39301 gatcagtttt tgattttttt aaaattcatt tttctaagac tggttagcag aaagaagaga
    39361 ataatctctt ctcttttttc tattatatat agattttctc tattttttgg taagggagct
    39421 gtttcaaaaa tggtaaagag cggaaatgtt gcgaatatca gttatttaat gatatgatat
    39481 tcggaaaaag aaatctcaaa gggaggtaac ttttatgggg ttgaatgaat ttattttagg
    39541 attgaacaat ttagagacga tcacaagggc acctggattt tttaaatata cagagcatac
    39601 agttgcagca cattcgtatc gtgtagcatc tattgcgcaa gttctaggag acattgaaga
    39661 agcgaatgga caaccgatcg attggaaact gctctatgaa aaatcattga atcatgacta
    39721 cacagagcgt tttattggtg atatcaaaac gccggtgaaa tacgcaagca aagagttaag
    39781 agggatgctg caaacagttg aagaaaagat gacggacgaa ttcatcaacc aagagattcc
    39841 agctgagttc caagaagttt atcgccgtcg gttgttcgaa ggaaaagatg aaaccgtaga
    39901 aggagaacta ttagccattt cagataaggt tgaccttctt tatgaatctt ttgatgaaat
    39961 catcaaaaat aacccggaaa aagtttataa ggagatgttc attgaggcag tgactacgat
    40021 taaaagcttt gaacatcggc cttctgttca gtatttcttt aaagagatat ttccggaact
    40081 attgaaccag gaattttatg gaaaagaggc gtttttagaa accgtctcaa cgattgtaaa
    40141 gaaagaagaa aaataaaaat gatatgagat gcagggggaa gtatagttga aaaagaatat
    40201 ggatgtcgaa tggggctatt ctggagacaa aggtccggaa aattgggcga gtttatgtga
    40261 ttggtttgcg agaggtgcgg aatttccatt gcagtcgcct attcatttga ccaaggggga
    40321 agaaaccaaa atagaaggag acacgctttc ctttcattat caaaagcaac ggtttactga
    40381 caaggaattc aaaaacacga ttcatttagt accttttgac caactcagtt atgtagagtt
    40441 caagaaagaa cgctattatt tgacagatat tcatttccac ttgccttctg aacatatcat
    40501 caacggaaaa caggaagata ttgaatttca ttttgttcat atgaacagca aaaaagagaa
    40561 tctagttgtg ggtgtcatgt accagctaat ggtacaggaa ggttggctgt ataatcaaga
    40621 aaatggggaa agttggaact tagaaaaaca tgagcattgg gtaaatccgg atgtgttctt
    40681 tccggagcaa aaaagccatt tccattatat tggctcattg acaactccgc ctaccagcgg
    40741 gccaatccaa tggtttgtaa tggatcatgt aggaaaactg aaccaagagt ttcttctccc
    40801 cttcgatggt cagtatgctc gaccgaacaa ccgaccaatc cagcccttga atggaagaga
    40861 aatttattat ttcgaggagt ttgagcaatg aatattcagc agatgaaata cgtggtcgct
    40921 gtagcaaaca atggtagttt cagagaagcg gcaaagaaaa tgtttgtctc ccagccaagt
    40981 ctttcagctg gtatcaaaga attagaaacc gagttaggga tcacgctgtt tactcgaacg
    41041 aatcgagggg catatttaac agaagaaggg caagacttct taaatcgtgc cgagaaaata
    41101 ttggtccagc ttgatacatt agaaactcac tatctaacga gtgaaaagag agacaatttt
    41161 tcgatcgctg cccagcatta tgatttctta ggtcctttga ccgcacagct actcgaggaa
    41221 ttcaaagacg agatcaaaca atttcggatc atggagacga caacagcgag agtgatcgaa
    41281 gaagtcaaag agcagcacag tgagatcggc attatttata tgaatgagca taatcaagct
    41341 ggtatcagaa gatatttaca acaaggagag ctaagtggcc gcagcattgg aaaattcaaa
    41401 acgcatattt ttttaaggca aggacatcca ttagctgaac acgcagagat caccaaagaa
    41461 gaattgttat cttatcctca agttcgtttt acacaggatg gaaataattt cccgtatttt
    41521 tatgaagacc ttatcgagat ccctgatcaa gaaacagtga tttatacgag tgatcgaggt
    41581 actttgatga atatcgttct tgaaactgat gcttacgctt caggatctgg tattgtgatt
    41641 ggagggattc gcgagcactt gcggttaatc cctttggcag aaggcgaact aaatgaattt
    41701 tatatcattt attcggcgaa acgtccgctc agtgaaatcg ctcaaagatt tatcaaagag
    41761 cttactcggt tactggacca gcagagctaa gaaaagcaga tgagaacgct gtttattgaa
    41821 caccgttgat caaaaaagag gggttgtgac agaattttgt cacaactcct ctttttgtag
    41881 caaacaattt ataactaatt gctgttgtgg gaaagtacgc aaatatgatt ctagaaacac
    41941 tcattgatta cctgttttgt ttcatagctg taattttttc ttcttctggt gtcacgataa
    42001 tcgtcttttg gttttcataa ataacataac caggcttggc gccatttggt tttcggatat
    42061 gctttacctg tacaagatca accggcactt gggcagagta tcggtatttt gaaaaatagg
    42121 cagctaactc ggcagcttct gtaattgttt catctgaggg cttgtcactt ttaatgatga
    42181 cgtgcgaacc gggtatattt tttgcatgaa gccaatagtc ggttttttta gctgtcttca
    42241 ttgtcagctg gtcattttga agattgtttt taccaacaag aatgagcgtt ccgtcagaag
    42301 aaaagtattg atcaggctga gatggtttct tacgtttttg tttttttaag ttcttctttt
    42361 tcaggtatcc ttctgaagtc aattcttctt taattgcttc gatatccatt gggccggcaa
    42421 tttccagttg agataagaca gattcaagat attggatttc atctttggct tcttggattt
    42481 gttcgccaat cagcttaaca gcgttcttca gcttttggta tcgatggaaa tatttctgtg
    42541 cattttgatt tggtgtcaat gccggatcta gtgctatttt gattggccga tcttcttcat
    42601 aataattagg taaaacaact tcttttgcgc cgcgtggtac ttgtgtcaga aaagtagtaa
    42661 gtagttcgcc atttctacgg taattctcag cgttttcgga ttctttgagt gtttgttcac
    42721 gttttttcaa tttattttta tttcgtttta gttcattttc gatttttcga atcagttctc
    42781 cgccttgttg ttttgcccga tctttctctg ctttttcgtg ataatagcta tctaataatt
    42841 gactgagtgt tggataagct gttacagcag atgcctgttc ggacaatacc tgataagcaa
    42901 tcggtgtgaa aaaatcttta ttttccgttt cgtaaaaagt aggaataggc tgatggctga
    42961 tggctgagaa aaattcgtcc catacaacca tcttttcatt aggacgttcc agtaatcttt
    43021 tggtcagctc ttgagcgata tcgaaaccga ttccctgaaa ctgacgttgg attgcttttg
    43081 gatctagttc agtttgggaa agccgctgaa agattttttc tttctcggag ctaaatgggt
    43141 tcaagacctc ttgttttggc ggagcaacat attctacacc aggaagcaaa gaacgataag
    43201 tgttttgaga gcttccaatg tgtttgatcg catccagtat ttttccagtt tctcgattaa
    43261 ctaagacgat cgtactatga cgtcccatta attcaacgat caatatgata ttttgtagat
    43321 cacccaactc gtttcgttta gcaaagtgga aatggatcac tcgatcattt tcgatttgtt
    43381 cgatgtcttc taaaatagcg ccatccaaat attttcgaag catcatcacg aagttaggcg
    43441 gattatcagg attctgataa tcgatttggg taatctgtac acgagcataa cttgggtgcg
    43501 cagataataa taatcgatgg tttttgccgc gtgaacgaat caccagcacg atttcatttt
    43561 cataaggctg atggattttc gagatccggc cagatagtaa cgtttcacgc aattcgttga
    43621 ccatcgcgtg ggtaaataca ccatcaaagg acatggttca ttcctcattt cttttcataa
    43681 gtatattcat tataacgaaa aaaagaagaa aaaccaattt ggccgaatag taaagcaaaa
    43741 gtctagaaaa atcttggact aattacataa aaaacagtgt ttctatcgtt gaaatccaaa
    43801 tttttaggt
//
LOCUS       DAJSVF010000017        43401 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSVF010000017 DAJSVF010000000
VERSION     DAJSVF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.017"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(87..1124)
                     /locus_tag="ON550_000890"
     CDS             complement(87..1124)
                     /locus_tag="ON550_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3953994.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1571..2554)
                     /locus_tag="ON550_000891"
     CDS             complement(1571..2554)
                     /locus_tag="ON550_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR3953995.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSSAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(2967..3737)
                     /locus_tag="ON550_000892"
     CDS             complement(2967..3737)
                     /locus_tag="ON550_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3953996.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(3730..4665)
                     /locus_tag="ON550_000893"
     CDS             complement(3730..4665)
                     /locus_tag="ON550_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3953997.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(4981..6630)
                     /locus_tag="ON550_000894"
     CDS             complement(4981..6630)
                     /locus_tag="ON550_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3953998.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAASFDEKRQWHVLG"
     gene            complement(6825..7721)
                     /locus_tag="ON550_000895"
     CDS             complement(6825..7721)
                     /locus_tag="ON550_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3953999.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNRWVDENEQKGQVFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(7714..8394)
                     /locus_tag="ON550_000896"
     CDS             complement(7714..8394)
                     /locus_tag="ON550_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954000.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILMDKESRDVNVNGVTVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(8395..9177)
                     /locus_tag="ON550_000897"
     CDS             complement(8395..9177)
                     /locus_tag="ON550_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954001.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(9170..10087)
                     /locus_tag="ON550_000898"
     CDS             complement(9170..10087)
                     /locus_tag="ON550_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3954002.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(10090..10305)
                     /locus_tag="ON550_000899"
     CDS             complement(10090..10305)
                     /locus_tag="ON550_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954003.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWQTTQPRLRDSLIFLEIQLE
                     NLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            11161..14388
                     /gene="fss3"
                     /locus_tag="ON550_000900"
     CDS             11161..14388
                     /gene="fss3"
                     /locus_tag="ON550_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3954004.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            14500..14814
                     /locus_tag="ON550_000901"
     CDS             14500..14814
                     /locus_tag="ON550_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3954005.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14827..15201
                     /locus_tag="ON550_000902"
     CDS             14827..15201
                     /locus_tag="ON550_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3954006.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            15202..15555
                     /locus_tag="ON550_000903"
     CDS             15202..15555
                     /locus_tag="ON550_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR3954007.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            15624..16640
                     /locus_tag="ON550_000904"
     CDS             15624..16640
                     /locus_tag="ON550_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3954008.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            17234..19108
                     /gene="ltrA"
                     /locus_tag="ON550_000905"
     CDS             17234..19108
                     /gene="ltrA"
                     /locus_tag="ON550_000905"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3954009.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            19234..19590
                     /locus_tag="ON550_000906"
     CDS             19234..19590
                     /locus_tag="ON550_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCR3954010.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            19635..20777
                     /gene="dcm"
                     /locus_tag="ON550_000907"
     CDS             19635..20777
                     /gene="dcm"
                     /locus_tag="ON550_000907"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HCR3954011.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            20767..21056
                     /locus_tag="ON550_000908"
                     /pseudo
     CDS             20767..21056
                     /locus_tag="ON550_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21312..22496
                     /locus_tag="ON550_000909"
     CDS             21312..22496
                     /locus_tag="ON550_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3954012.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            22493..22606
                     /locus_tag="ON550_000910"
     CDS             22493..22606
                     /locus_tag="ON550_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3954013.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            23376..25286
                     /gene="ltrA"
                     /locus_tag="ON550_000911"
     CDS             23376..25286
                     /gene="ltrA"
                     /locus_tag="ON550_000911"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3954014.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            25390..25614
                     /locus_tag="ON550_000912"
     CDS             25390..25614
                     /locus_tag="ON550_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954015.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            25627..26130
                     /locus_tag="ON550_000913"
     CDS             25627..26130
                     /locus_tag="ON550_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3954016.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            26257..27294
                     /locus_tag="ON550_000914"
     CDS             26257..27294
                     /locus_tag="ON550_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954017.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            27309..29006
                     /locus_tag="ON550_000915"
     CDS             27309..29006
                     /locus_tag="ON550_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCR3954018.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            28999..29460
                     /locus_tag="ON550_000916"
     CDS             28999..29460
                     /locus_tag="ON550_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCR3954019.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            29473..30333
                     /locus_tag="ON550_000917"
     CDS             29473..30333
                     /locus_tag="ON550_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954020.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            30371..30760
                     /locus_tag="ON550_000918"
     CDS             30371..30760
                     /locus_tag="ON550_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3954021.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            30744..33194
                     /locus_tag="ON550_000919"
     CDS             30744..33194
                     /locus_tag="ON550_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3954022.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            33199..35307
                     /locus_tag="ON550_000920"
     CDS             33199..35307
                     /locus_tag="ON550_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3954023.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            35304..36308
                     /locus_tag="ON550_000921"
     CDS             35304..36308
                     /locus_tag="ON550_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3954024.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            36327..37238
                     /locus_tag="ON550_000922"
     CDS             36327..37238
                     /locus_tag="ON550_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3954025.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            37425..37712
                     /locus_tag="ON550_000923"
                     /pseudo
     CDS             37425..37712
                     /locus_tag="ON550_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(37900..38115)
                     /locus_tag="ON550_000924"
     CDS             complement(37900..38115)
                     /locus_tag="ON550_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954026.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(38374..38541)
                     /locus_tag="ON550_000925"
     CDS             complement(38374..38541)
                     /locus_tag="ON550_000925"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3954027.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(38886..40325)
                     /locus_tag="ON550_000926"
                     /pseudo
     CDS             complement(38886..40325)
                     /locus_tag="ON550_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(40873..41544)
                     /locus_tag="ON550_000927"
     CDS             complement(40873..41544)
                     /locus_tag="ON550_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR3954028.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(41893..43344)
                     /locus_tag="ON550_000928"
     CDS             complement(41893..43344)
                     /locus_tag="ON550_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3954029.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT        14075 a         7875 c         8548 g        12903 t
ORIGIN      
        1 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
       61 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
      121 cctaacatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
      181 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
      241 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
      301 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
      361 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca
      421 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
      481 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
      541 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
      601 agttgattca aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg
      661 cgattggcat tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc
      721 aatgaggtca acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg
      781 acaatacgtt cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct
      841 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact
      901 ttcccgactc catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga
      961 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
     1021 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac
     1081 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
     1141 cttcatcaag ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt
     1201 tccacctttt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
     1261 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc
     1321 taccaactct tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa
     1381 ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc
     1441 aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt
     1501 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     1561 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaaa gtgtctaaga
     1621 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     1681 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     1741 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     1801 tttctgtatg ttccctcgga tctttttcaa atgcataagg attcttgaaa atccctcggc
     1861 caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg
     1921 ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga
     1981 ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     2041 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     2101 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg
     2161 cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt
     2221 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     2281 ccatagcact gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     2341 tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat
     2401 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     2461 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     2521 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     2581 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     2641 gcaaagtgaa cagctcagaa ggtttgtctg attgaacaaa aagaggactg tgacaaaaat
     2701 cttcacagtc ccttttccgg aataaatggt gttctcctgc caactcctcg gtcacaagtc
     2761 aaaatattca tcaaacatga gaagctattt ttgtttggct gttcttatta cttgatgcag
     2821 aacagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcgatattcg
     2881 ctcaaaaaac aacaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggtc
     2941 aaaacgctga tttcacaacc tcttttctaa cttctcaatc ctcttccttt attgatcaaa
     3001 taccaagtaa cggcgtacaa aacaacgatg aataacacaa gtacgatcat cgatgttgct
     3061 actggcacat cgatcgttcc aaggaatccg taacggaaac cggagatcat ataaacgatc
     3121 ggattgactt tcgaaacagc ttgccaaaag ggcggtaaca tagagatggc atagaaaacg
     3181 ccacctagat aagttaacgg ctgcaagaca aatgtaggta cgatcgatac atcatcataa
     3241 gactgagcaa aaatcccgtt gagcagtcct gctaacgaaa acaagattgc cgtcatcaat
     3301 aatgtaatga tgaccatgaa ccatgaatac acatgaagtg gtacaaagaa caaggaaatg
     3361 atcgttacga gagagccgac gagaatactg cgacccacac caccaatcac aaagccccaa
     3421 ataatcacat gcgtaggaac aggtgctacc aagatttctt cgatattttt ctgaaatttt
     3481 tgagagaaga aggacgaaga aacattggca tacgaactag tgatagcaga catcataatc
     3541 aaaccaggaa cgatgaattc catatatgaa aaaccaccca tgtcaccgat ccgcccaccg
     3601 atcatttttc cgaaaataac aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt
     3661 tggacccata tacgcaaata gcggttcgtt tccttgatag ctaaactttt taatgcagta
     3721 aaataaagat taaacatttt tctcctccgt atgattattt tcttctgtga ttttcaagaa
     3781 cagttcttct aaacgattcg acttattcct catagaaaga actttgattc cttgtcttgt
     3841 aagctgatcg aagattccgt tcaccccttg atttcgttca acttcgacag cgattgtctg
     3901 ctcatcttca aatgtacaag catatccttc aatcacaggt ttggtatcat atggcgctaa
     3961 atcaaagata aatgtttcaa attgcagttt tgacagaagc tctttcatac ttgtattctc
     4021 gatcagttca cccgattgga tgattccgat gttgcgacaa agcatttcag cttcttctag
     4081 ataatgcgtc gtcaaaatga tcgttgtacc attttcgttc aattctcgta gaaactccca
     4141 catctcgcgt ctcaactcga tgtcaactcc tgctgttggt tcatctaaaa tcagcaaacg
     4201 tggttcatgc atcagtgctc tagcaatcat cagtcttcgc ttcattcccc cggaaagcat
     4261 acgtgcacgg acatttctct tttcccaaag attggactgc ttcaaatatt tttcactgcg
     4321 tttcatcgct tctttgcgag aaacaccgta gtaaccggct tgattcacga cgatttgctg
     4381 gaccgtttca aaagggttaa aattgaactc ttgtggaacc aatccaatct gttgctttgc
     4441 tcgaactaga tccttgtcca gatcataacc gaagacattc acttttccag atgttttatt
     4501 gactaaagaa gtaataatcc cgatcgttgt agacttccca gctccatttg gccctaatag
     4561 cgcatagaaa tctccttctt tcaccgaaag atcgattccc cgcaatgctt cgaccccact
     4621 tccatagatc tttttcaaat tatttatttc aagcgcattt gccatttatt tcctatctcc
     4681 ttatagacat gattctttat tgtatcatgg aatacagcag atgaaaaaac aacttggttc
     4741 cagcgaaaaa tcaggttgct gggctcagga aaatctccta atgaaagaca tcaatcggac
     4801 gaagtcagac aaccttttca gaaaaaacaa atcgaagata aaggattact attcatatgt
     4861 gcaacaaaag gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
     4921 ataaacggcg gaagcatgag ccaagatttt tgatccaata aatactttcg atcacttcgc
     4981 ctatcccaat acatgccact gccgcttttc atcaaatgac gcggcttccg tttttgcggt
     5041 ttctactaaa gtctctgggt aatccaaaac tttcagcaag gcaatcgcat ttcgggtcct
     5101 tgaaggtcct tcttttaatt tgaaatcaaa actgattcct tttccttctg ttacttgttc
     5161 ttcaaagtgg acattctcac aataattctt taaaatctct gtcaattcga tatcatgtgt
     5221 ggcgacaaat gctaaagatg gatactccgc aagccaccga acgactgaag cggaagaagc
     5281 aatccgttcg atcgtatttg ttccttttaa aatctcatct acgaagcagt agcagcgttc
     5341 tttggaagcc acttggtcca gcaaacgttt gatcgatttg atttctgaaa caaaataact
     5401 gtcgccttca aataaatcat cttcgattgc catggaagtc aacacatgtc caggttgcag
     5461 ggtaaactgt tctgcaatcg ctgtttggat cgtctgtgct agaatacagc tgatggctat
     5521 acttttgaca tacgtagatt ttcctgatgc attggaaccc gtgactaatg tattcttctg
     5581 ccagttgact ggattcacta ccgcttcttt taacaatgga tgatagatat ctttcgctgt
     5641 cacaccgccc tcagtaaact ctggtcggca agtgatcggc atataggtgc gataattcaa
     5701 aacggctgct gcaacttcca attctcccag caattcccac agcttgatgg cttcagttga
     5761 atgcttttct aatttagtga gaacgaaatg ataggagata aatggtagca tgaacatgat
     5821 gttgacatat tcaaaaatca attctgcttc actcccgctc ttcattcgaa atgaaaaacc
     5881 taatttaggg atgtctctta atggtttagt ttgctgtttc agctcttttt gaagtggtgt
     5941 cgtcattttt gctaactgat tggcggctga aatcgttgaa accaaataac tcatgctatt
     6001 cagctctgtt tctagtgttt gtttcttcac tagataatag accgtattga atactaagcc
     6061 accgattgct agatagattc ctagtacttg acctaaaaga agcaaaccta cacccaccag
     6121 cggaagcaag ccaagaaaaa taaataaacc tacgcgtcca atctttttac tttttccatc
     6181 cgctagatat tgttttgcaa aattatgatc tttttttcct aaacgagcga attgatattg
     6241 gattctttct ctcagttgag gattttcctg ataaaaagca attagttttt ctaactgata
     6301 atcttctcca aaatcaaagc tacgtaaacg ctgatacaac gcttctgaac ctacactaga
     6361 ataggtactg ttgatccctt caaatacttc gaacatatcc aagtcatacc aagtaagatc
     6421 gtcgatttca ctatcccatg aacggaattt tttctccgtg agccacgcat ccttcaaact
     6481 ttcttcttta tcaaatctcg gttgataagg aatttttccc caattactac gaactgtttc
     6541 ctttactttc aaacgtgaaa aaatgtctaa aacaactaat aaaataacaa ctgcaataat
     6601 aattaaaacg ataaattctc tttggcccat atccctctcc gttccttgat ttgttagttt
     6661 tagtatagca ctgttgcgct atttgaaaag ggggtacaaa aataactatc ttcgttacct
     6721 aattcgtatc aatcacacca aaataaaaac accttccaaa aaagaagatg tctttaaaac
     6781 gagatacaat tttttgtcat tcacttcagg taaattagag ccaattaaac cctccgtaac
     6841 ttgacggaga tggagaagat gcccccgtct aatctagcgg tcatctctcc tcccattcct
     6901 tccaccaatt tttttgacag gtaaagcccg attccgctag attccgtatt tgtgcggctg
     6961 gtattttcgg tataaaaacg ctggaagact ttttcaacag aggtttgcgc tcctggtttg
     7021 ataggattgg ccactgttaa tcgaatgaat ggatcctctt ttgaaaatgt cacctttgct
     7081 tgtccatctg cataacgcaa taaattctgt atgatatttt gcagcactcg cagcaacatg
     7141 agcggatctg ctaatacagt tgtatttatc gagtgttcag cgaacgctac ctcgatattt
     7201 ttctcagaaa attgttcata aaaagagaaa aatacttctt ccacctcttt agaaaaagaa
     7261 agcgtatgga gatccatttc tttttgcttc gtctctattc gagcaagatc ataaaactga
     7321 tccgtcatac tgagtaatct tgcaacagat tgtttgatga tcgttaaata cttctcccgt
     7381 ttctccgtat cctctacctg ttccagtaat tgtacataac cgttaatagc agtcaaagga
     7441 gttctgagat catgagaaag gccgacgatc gatagtctga tgttttcttc catttcttga
     7501 aatacttgtc ctttttgttc attttcatcc acccatctat tcagtgcatc tactaggtcc
     7561 attagattct tttctcgaaa ttcgactgaa agacgaccac catattcttg tcgttcagaa
     7621 agagtggcga ttttcttagt taatcgcttg atttcccttt gcaggaagaa aataaaactt
     7681 cccataaaga taacaacgag aagcaacagc cacttaatca aatctaaacc ccactcccca
     7741 caccgtatga atatacacag catgtgcctt tgctagcttc cctcgaagat tggaaatatg
     7801 catattcact gttttctcac tatcaaaata tggttcttgc caaactgttt cataaagatt
     7861 tgctcgcgaa aatacttttt tcggatgttc taaaaaaagc ttcagcagat cgaattctct
     7921 tcctgtcaaa tggacagtca caccattcac atttacatcc cttgactctt tgtccattaa
     7981 gatttccttg taatagatgc gttgcgacag atcttttact tggacagaag tctgatgacg
     8041 aagttggatg ccgattcgta acaacagttc ttcgatatca aatggtttcg tcacatagtc
     8101 atccgctcct gctgccaaca aggaaacttt atctctttga tcgctttttg ctgaaagaac
     8161 gatgatcgga acattcgatg tctgtcggat ttctgctaac aatgcttcac ctgaaagccc
     8221 tggaagcatg agatctagaa ggatcaaatc gacaggatat gtgcttaaca ataattttcc
     8281 ttccgtacct gaataagcat caagaagttt gtatctctgt tcaagaacct cctccagtaa
     8341 agaatgaaca gcttcatcgt cttcgataat caaaatagtt tccagcagga ccacctactt
     8401 aatgtcagat ttttggaaaa taaacattcc tagagctgat gataccacga ttgttactag
     8461 actgaccaga atcattttcg gtgcttgcga ataagttgct tgttgcgcaa agtccgtata
     8521 catgagccac gtttccgtaa accactccgt tagaaattcc aagcctttga tttttgcgaa
     8581 aacaccggga acgaaggccg ttcccatgat ataaagaaaa gaaagaagca aactgacact
     8641 ggtacttcta gtgatgaatg tcaaagccac tggaacagat aatgtcgcta atatcagcag
     8701 aagcaataca ggaaatactt ttagaacata atttccatag tttttgattc cctcaacttc
     8761 accaaatccg aaagcaagtg tgtaagcaat catggctagc cccgtaatca aaaccacgcc
     8821 aagtgttgtg atcaaaagcg taacgagaag tttcgacaca taataagtca ttcgattccg
     8881 tcctaaagat aacgtattgc gaatcgtacc gttttgaaat tcttccccaa tataaaagca
     8941 cgcaaatgct gcgaaagcca gaagaatcca tgcagccatt ttttccacca ttaaaatccc
     9001 tgttgcaccg ttgatgactg tatccgttgc ttggtttaat tcattgccgt attttgtatg
     9061 catataccat tcgttccaag aataaaaaag cggaagtacg accatcagtc ctagtgagac
     9121 ccacatttta cggctcttcc atacttttat tttttcagca gttaatagat taaacatgtg
     9181 gattgcctcc agttaattgt aagaaataat cttccagttt ttgcccaact aggtggatac
     9241 catccacagg aacttttgcc tctaccaatg cttgattgat ggctgctact tggtcaattc
     9301 cttcaaaaag ctggataaca cggctagatt ggacaaaata atccctataa ccaagagagt
     9361 cgagaaccgt tactgcttgt tccgtcgccg aagtgtcaat ttttataaat tgacgagatt
     9421 cttgtgccaa ttcttctttc gataattctt tgatcaagtg tccttcatgc aaaataccgt
     9481 agtgagtggc gatctgtgac aattcatcta gcaagtggct agaaagcaag acggtgatgc
     9541 cccgttcttt taccaaacgt tggatgattt ctcgcatttc tgccatccct gctggatcca
     9601 tcccattcgt cggttcatcc aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa
     9661 tagctaaacg ctggcgcata cctaaagaga aattctttgc tttctttttc cctgtatttt
     9721 ccaatcgcat cattttcagc aggtcttcga tttctctatc cgacacaccg ccgttcgctg
     9781 cttggatacg taagttatct cttgcagtta attctggata aagagcaggg gtttcaatcg
     9841 attgacccat acgtcgtctt gctgcctgca aatccgtcgt tccaaataat tcgatcgaac
     9901 cttcatcaat cgagatcaaa cccataatag ttctcataaa ggtagactta cccgctccat
     9961 tctccccaat caaaccatag atcattccac gtttgatttc gatcgatact ttatctaatg
    10021 ctttggcagc accgtatttt ttggttatac cagttgcttt caagaccgtt tcatttccaa
    10081 aatccatact tatccttctt tcttattgat aagtgaagta tagttgacgg gtataaagaa
    10141 agcagccaga aatgtataaa gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag
    10201 actgtctcgc agacgtggtt gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa
    10261 tccaagtgga ctggccacca tggtaatcgt tgttatcgtt gacatcagac gtccttgtat
    10321 atgcgttgga acttctcgct gaagctagac catataaaaa gcaaaagaaa acattaatcc
    10381 ttaaaaacgg acttatgaaa aataatacat gccgtttggg atttacaatt agctaccaaa
    10441 taagaggtta tgacaaaaga cttttcacaa tcccttcttc cgaataaacg gtgttctcct
    10501 gccaactcct cggtcacaag tcacaatctt catcaaacat gagaagcgat ttttgtaaat
    10561 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcagaagcgg ataaataata
    10621 ttgtttgaag atacttgatc tttttcccgt ttgtttatag atcgaatgat attatagacc
    10681 aagaatccta cctattaaca tagctaaaaa cacaaaaagg cactttccaa attgtggaaa
    10741 gtgccagaaa agttaggatt aaggtaactg cgctcagcat tgcttcgccc agtacgattc
    10801 tcgtaactcg tgaactcgaa cgataatcgc aattaacgtt ttgagaactg tgaagcttta
    10861 cgggctttct taagacctgg ttttttcttc gtatcattga ataagtttga aaagccttga
    10921 tttataaagg tttttccgta aatatatttt gttatatttg attaaatttg aaatgtttgt
    10981 gttctaaaat gtgttctaat ttaaaggcag tatatttcgt attttatatt aaatttttag
    11041 gctcattcat tctcaaatcc attctattta gtcaataaat attttaaaaa gactttataa
    11101 acaaagatgt tcaagcgagc atcttatttt tttgtgccta atttgaaagg agaacatttc
    11161 atgtataaaa aacaaaaaaa gaaattatgt gtattaacta ttttgagttt ggctgttcaa
    11221 tcaattgtag gagcaattgc tcctacgatt gcttttgctg atgaaattac ccatccacaa
    11281 acggtaaccg tagaactaga tttagcacat caatatgctg ttgaaggaac ttttagtgat
    11341 ggtcgtccaa tgtcagaagt cactgttccg cattatgcag tgtacaacgg cgttaaacaa
    11401 gatattttct gtgttgaacc aggagtcccg atttacaatg agttcactcc tggatatgag
    11461 aagaacccgt taccagatat gtccgataaa gccaaattag tttccgtttt atggaaaaaa
    11521 gtaggtacag atgcagatac tcatattgtg gcacagaaga tgatctggca agaagttaat
    11581 gggtataccc ttcactcaat aaaacgatca gatggtagtg ccgtaaatat cgcagccatt
    11641 gaggcaaaaa tcaatcaagc cattgccgat tatcagaaga aaccaagctt ccataatagt
    11701 acggctaaaa cagtattagg gcaatcgact actgtgacgg atacgaataa tctgaatttg
    11761 tcagagttcg atgaggttgt ggaaaatacc gcaaacattg attatcgtgt gaatggcaat
    11821 cagttgataa tcagcccaaa tgccaattca aaagaaagtg gtgtgttgac gcttaagaaa
    11881 tctgctggta ccggaacacc tgtcgcctat aaaaaggctg gccaacagac tttaatggcc
    11941 ggggcgatcg ataagccgaa cacttatacg gtcaaaattg atgtggaaat ggagggttct
    12001 ttgaagatta aaaaagttga taaagaatcg ggtgatatta taccaggaac ggttttccat
    12061 ttagactttg gaaaaacttt acccgcaaaa gacgtgacca ccgataaaga aggcattgcg
    12121 accttagatg agattcctca tggtacaaag gtaaccatta ctgaaaaatc ggtgccggcg
    12181 ccttatacga ttgataccac acccatgact gccacaatta aagcaggtga aaccatttca
    12241 gtgacttcaa aaaatacgcg agaaaaaggt caaattattc tggataaaac cggaattgaa
    12301 acagggagtg atctttggaa cgataattac tctttagcgg gcaatacgtt tgccattcgc
    12361 aaggatagcc caactggtga aatcgtccaa gaaatgacca cggatgaaaa aggtcatgca
    12421 gaaacaccaa aagagattgc caatgcgttg gaattgggaa cctactatgt gactgaaacc
    12481 aaggctagtc atggctttgt gaatactttc aaaccagtaa aagtcgaatt gaattatgtc
    12541 aatcaaacag tggctcttgt taccagtaac gtaaaagggc aaaaccaaga aattactggt
    12601 gaaaccactt tgacaaaaga agacaaagat accggtcatg aaactcaagg gaaagctgag
    12661 tttaaaagag ctgaatacac cttgtttact gttaaggatg gaaaagcggt caaatggacc
    12721 gagaacttta aaccagaatt gttgaaggga acgaaagctt ctgatgaaac cgtaactttg
    12781 actttagatg aaaagaatca agttgccgtt aaacacttag cgattaatga gtattactgg
    12841 caagaaacca aagcgcctga aggatatacc ttggatgaaa caaagtatcc tgtatccatc
    12901 aaaaaggtgg atgatagcga aaaaaatgct gtgattactc gagatgttgc ggcaaaagaa
    12961 caagtgattc gctttggctt tgatttcttt aaatttgctg gatcaactac tggcactgcc
    13021 gaaactggct tcaatgatct aacctttaaa gtgtcgccat tggaaggaac caatgaaatc
    13081 accggtgccg aagatgaagc gaccacagct tataacgagc aattagggtt tgatggttat
    13141 ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga agccccagaa
    13201 ggttttcaaa agattacacc attagaaatc cgttctactt ttaaagaaaa taaagaggat
    13261 tataccaaga gtgattatat ctttactatt accgaaaaag ggcaaaagca accgattaaa
    13321 acggtaacgg tcccttatgg gaatctaacc aatgagaatt tctcagtcag tttgaaccgt
    13381 ttgatgcttt atgatttgcc tgaggaagaa gatagcttaa cttctcttgc gacttggaaa
    13441 gaaggagata aagaattgac tactcttgat tctaccgagc tagtcgataa attgagctat
    13501 aatctacatg aaatcaaaga agactggtat atcgtagctc aagccatcga tgtagatgct
    13561 acaaaagctg cccaagaaaa agataaaaaa gccaaaccgg tggcgattgc ggaaacaacc
    13621 gcaactttag cgaataaaga gaaaacagga acttgggaga ttctgcataa attaactgtc
    13681 gaacaagttt tagacaagac cattgtctta ttcaactatg tgtatgaaag caaagaagcc
    13741 tatgaagctg gtgatgagcc ggtagcgaag gatgctagct tgaacaatca agcgcaaact
    13801 gttaattgta tggtggaacg tcatgtttcc atccaaacca aagcgcactt agaagaaggt
    13861 tctcagacct ttactcatgg tgacgtggta gatatgtttg atgatgtatc cattacccat
    13921 gatgtactag atggctcaaa agaagctttt gaaacaattc tgtacgcttt atttccagat
    13981 ggtacaagta aagaaatttg gaaatctggc aagattgatt acgaagtgaa tgacaaagaa
    14041 tttaccaaaa ccgtacttgc ggaaaaagtg gatacaagta aatatccaga aggtacaagt
    14101 tttaccttca aagaaattaa ctatgacaaa gatgggaata tcaatggaaa acacaatgaa
    14161 gatttgaaag aaaaatctca aactttaacg cccaaaaaag ttccgacaac accaagtaca
    14221 ccagaacaat ctgaaatgcc aactgttcca agtggctctc aagaatctag tcctacagtg
    14281 aaggcattcc ctcaaaccgg ggagaaaact tccaatgtgc tactgttcat tggctttagc
    14341 ttgatctttg cgacggctgg ctattatttt tggaatcgtc ggaactaagg tgataagatg
    14401 aaacatgtaa atagtagaaa acaatacgcg cgaccgcctc ctaaaaaagt ggtcgcgcta
    14461 tttttgtatt caaaactaac aatgaaaaga ggaaaaaata tggaattgaa atttgttgta
    14521 ccagatatgg tcgagacgtt cggaaaacta acatacggtg gtgaaggtga agttctatcg
    14581 gaagggtatg gtcgaaatac tacaattgtt ggtcgaagtt accatctgtt ttcaaataaa
    14641 caacgggccg atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgac
    14701 caagatcaac cactaaaagc tgtaaatccc cacttaattg ccaaaggcta tgaaattgag
    14761 aatcgtgggt ttaccgatta cgtgttgtat gttgatgatt tagtgaaagc ataggaggaa
    14821 aagaagatga gattaacaga aggtattgtc gtagattcag gattaacctt tggcaagttg
    14881 cgtttttccg cattacgtcg agaagtacgc aagcaaaatg aggacggaac ggttagtaac
    14941 gaggtaaaag aacggaccta taatttgaaa tcctccgcgc aaggtcgaat gatccaagtc
    15001 agtattccag caaatgtacc cttacgcgag tttgcttatg atgcagaggt agagctggtc
    15061 aatccaatcg tggatacggt cgccaattat gtttttcggg aaggaaccac cgttaattgg
    15121 tttatcaagg cagatgattt agtcttgaaa cgacaactga atcaaggaaa acctacgaac
    15181 caaaatgaag gaaagaaata ggtgaagaat atggaaatta atgttgaaca tattttagat
    15241 tgtctcgatc aatttggcaa aggtgagcta acgggggaac aactcactaa cgcattaacc
    15301 tatgatgaaa aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgcc
    15361 aaagaagact ttgatgtact ggattggtta ggggaagaag agtctttctt tatggttgtc
    15421 gaggtaaatg aaagtttatg tcggcaggca gaatctgttt tggaagaaat cgatgtggag
    15481 atgccggatg ccatcgaaag atttttaaaa caattagtgg aaacgaaaca acttcccgta
    15541 gtagtaaatg actagcataa ctttttgaaa ttgcccaatg aaatcctatt gggctttttc
    15601 gactggaggt ggaaaccatc aaaatgtaca aaggacatcg catacgcgca ggagatcaac
    15661 acttgattta tcactttgtt cttggctggc taatcttttt gttcattggt tggatgagcg
    15721 tcttttattt acaagaactt agacaatttg atatttctaa ggtatcattt tccactagtg
    15781 aaattggctg gtctatgaag gatgtaatct gcttgctagg aagtctcgct ctttcaggaa
    15841 gcatgatgtt actttatata cacttttttc aggatcattg gcgaagtctt tggcatcgac
    15901 aaaagttagc tcggatgatt ctagaaaacc attggtatga agtaaagcag actcaaggtg
    15961 aaggtttttt caaagatttg aatagtagtt ggaccaaaga gagtatcagt tacttcccca
    16021 aaatttatta tcgaatgaaa gatggcttac tttctattcg cgttcaaatt tcactgggaa
    16081 aatatcagga tcagctattg aagttggaaa ataagttaga aagcggactg tattgtgagt
    16141 tagtagaaaa agaactcaaa gactcctatg tggaatacac cttgttatac gatatgattg
    16201 ccaatcgaat tgggatagaa gaaatagtgg cagaaaatgg ggcgttgcga ttgatgaaaa
    16261 atcaagtatg ggcgtatgat tccttgccgc atatgttaat tgctggcggc acaggtggtg
    16321 ggaagaccta tttcttactc accattattc aagcactatt gaagtcagat gcagaattgt
    16381 ttatcctcga tcccaaaaat gcggacctcg ccgatttagg tacgataatg cctcacgtat
    16441 actcccaaaa agaagaaatt tctgaatgtg tggaagattt ttacgaacgc atgatagctc
    16501 gtagtaggtc aatgaaagaa atgcccaact acaaaacagg agagaattat gcgtatctcg
    16561 gacttccgcc caacttttta atctttgatg aatacgtggc ttatatgggt gcgaatcgtt
    16621 ttcctacaag catcgaatag atgtgaaatg gaagcctatt gagattatca atagtgacaa
    16681 ctaatgtttt gagagatgga atgaaagcct tcaagttgag ctgaaacatc cccgctaata
    16741 ctcctacatg ctctaatcaa tgaagtagat tggttttggt atgaagctag gtgaagtcgt
    16801 ctgaaatctg cctaagtgct tgagcataag gctgactata gggcgggcgg ctgaaaaaga
    16861 taactatggt aagaatgtct gaaaatgact gacgaatctg cgaatgtacg ggtctaaaag
    16921 gctgatctta ttgaaagata ggaacatacg gacgtatggt gagataccga caagtaaggt
    16981 tccatgatat gaaaatcggg atagctaacg aagaagctat aatttcacag gctcatagta
    17041 gatacctaag gttatacatg atagctaaaa acattggaac gatgtaagca gaaaactatc
    17101 aattaaatga ctacttctag ataggtgcat ataagtaata acgaaattgc tttgtttctg
    17161 tgaaagtaag ggcacagtac cgatgaagcg tctaacaaat gtggagggat agccctaagt
    17221 ctatttaata acaatgaatg ttgaaacaga tgaagttcaa ggatctggta agaacgtggg
    17281 aaaatccgtt atggaggtgc cacgcttgaa cacaaaaata cgttactggg aatattatga
    17341 tctacaagaa acattcgatc agctatttac tcaaagtaga aacgggaaaa agttttatca
    17401 attatatgag ttaatcattt ctgaaaataa tattctttta gcataccgta ctatcaaggc
    17461 aaacaagggg tcttcgacac caggaacgga ttccttcaca attgataact ataaggaaat
    17521 gaaccaagca gagtttatcc atcttattct cagtcattta gaaaattaca aaccaaaatc
    17581 cataaagcga gtaatgatac ctaaacccaa tggtgagaaa cgcccattag gaattccatg
    17641 catgattgat cgaatgatcc aacagatgtt caagcagata ctggagccaa tatgtgaagc
    17701 taagttttat gaacatagct acggtttcag accattgaga agtgcaaagc atgctctagg
    17761 tcgcattatg tatctcataa acatcagtaa aatgcactat gcagtagaca ttgatatcaa
    17821 aggtttcttt gataatgtaa accaccgttt actgataaaa caattatgga atataggaat
    17881 ttgtgacaaa caagtcttag ctattctttc aaaatctctg aaatcaccca ttcaaggaga
    17941 aggtatcccg agcaaaggaa cgatccaagg aggtatcatt tctccactat tatctaatgt
    18001 tgttctaaac gacttagacc attgggtctc aaagcagtgg catacctttg aaacaaagta
    18061 tccctatact aaaggttaca ataaatttcg tgctctaaga gatacaaatc taaagcaagg
    18121 ctatattgtc aggtacgcag atgactttaa aatcatgact aatgattatc cgacagcatt
    18181 aaaatggttc cacgctgtca agctttacct aaaagacaga cttaaattag atatttccaa
    18241 tgagaagtct aaaatcgtca atttgcgtaa atgtaagtcg gaatttttag gattcgctat
    18301 ttgcgtgaaa caaaaaggga aaaaatgggt ttgtaattca cgtatttcta acaaaaaaaa
    18361 ggatcagatt aaagaagaga ttaaacaaag aatcaaagat attcaaaaga gtcctacagc
    18421 acaaaatgct ctcctgttta atgctctcgt gctcggtgtt cataattatt tcaggtatgc
    18481 cactcatatt aatttagatt tgaataggat gaactacgtt ctttcattca ctacttacaa
    18541 ccgattatca aattgtagta caagatgtta ccccaacaaa gcttccccaa cttacaaaaa
    18601 gttttactca acaacgatga aaacgtataa agtagctgga gtctatttgt accctctttg
    18661 cgacgtatct acaaaaacaa tttacggatt taatacggag gatacaccgt ttactccgtt
    18721 tggtcgtcaa agattggagc acaaaagcct acagtctctt gtgttccaag aattaagaaa
    18781 attgatggaa tcgaaaatac cgaatagaac ggttgagtat ttagatagta gaatatctcg
    18841 ttactcaatg aaatcaggca agtgtgagat tacaaaacaa tttttgccag caaaagccgt
    18901 tcactgtcat cactatttgc caaaaagtct tggtggagac gacaaattcg ataacttgag
    18961 aattattcat aaggatatcc atcttctgat ccatacaaca aataaaatga taatcgatca
    19021 ctacgtgcat gaactgaaac tggtgccaga gcaaatagca aaaatcaacc tttatcgaaa
    19081 gatgtgtaat ctgcaaaaca ttcaatagaa aataaataga tggaacgccg tatgaggtga
    19141 aagtctcacg tacggtgtgg agcgggggaa aagatggaga ttatatcaaa ttcttaccta
    19201 tcgctataga tgttaacgac aaaagaaagt gcggtgattt tgaataagct taaacaaatc
    19261 gtgatgttag gccgtcaatc tggtttcttt ctgattctag cttgtcaaag gccggatgcg
    19321 aaatatctag gagatgggat tcgcgatcag tttaacttcc gtgtggcttt gggtcgtatg
    19381 agtgagcttg ggtattccat gatgtttgga gaagtcgata aaaatttttt tatgaaacgc
    19441 atcaaaggac gaggttatgt agatacaggg ggcagtgtga ttagtgagtt ttatacgcca
    19501 cttgtaccaa aagggtatga tttcttaagg gaaattagca acattgttgg attaaccgtt
    19561 catacggaaa gagaaaataa tagtgtgtag cctaggaata tcaataatcc tgggcttttt
    19621 tattgaggtg atttatgaca tttttagatt tattcgccgg gattggtggc tttcgcttag
    19681 gtatggaaca agccggtcac cagtgtatcg gtttttgtga aattgatgaa tttgctcgga
    19741 ggagttataa agcaatccat gatacaagga aggaggtgga aatgcatgac atcacaagtg
    19801 tatcagacga atttgttcga tccctcggac cagtggacat cctttgcggc ggctttccgt
    19861 gccaagcttt ttcaattgcg ggaaagcggc aaggattttc cgatactcga ggtactttat
    19921 tctttgaaat cgctcggttc gccactcttc tccaacctaa gtttctattc cttgagaacg
    19981 tccggggatt gctcaatcac gaaggagggg ctacgttcga gacgatcctc cgaacgttgg
    20041 atggattggg gtatgatgtg gaatggcaag tgcttaactc aaaggcctac gttccccaaa
    20101 gccgtgagcg gatcttcctt atcggacatt ctcgagatgc atgtaccgaa caagtatttc
    20161 ctatcattgg atcgtcttca acatctgatc aaaacatcag aaacttggta aatatcaatc
    20221 cttccaatcg aggaatgggt ggccaagtct atggttcaga tagcgtagca ccaaccttga
    20281 ccggtgatga aggaattaaa attgccttac cggtaaagga tgggatttct gtagcgggta
    20341 tgttgtcggg aaattttgaa caaggcaata ggatttatga ggtaaccgga acagccccca
    20401 ctttgtcaac gaaacaaggg ggaacgaaga ttatgattcg gaaaaatgaa acagaatatc
    20461 aagaggtgaa gccaggggac agtgtgaacc tggcttttcc taattccaca agtcgccgag
    20521 gtcgtctggg aaaacaaagt gtgcatacct tactaacagg agaccaacaa gcggtagtca
    20581 cggatcagta ccagattcga aagttaacac ctagggagtg ctggcggctt cagggctttc
    20641 cagattgggc ttttgatcga gcttctcaag tcaattcaga tagtcagttg tataaacagg
    20701 ccggaaactc agtaacagtt ccggtcattt ttgatattgc tagaagatta aagggggtaa
    20761 aagcatatga tttatgacga gttgattgga gaaatctact gggtgattgg caagattcag
    20821 gcagatccga aattggaaga agaactgtac cggctcaatt ttgatattcg caaaaacggg
    20881 gtgaaagtcc aggtgattcg tatgtagtgg atgaggaaac ggatgctcga atggagctca
    20941 atcaagtcat cggtgaattg gaacgaattg cggaccttgc caaagaacct gatatccgcc
    21001 aatacttgtt tgagataaaa gcagagttgg aaattgaggg tatcaccgcg gagtaatagg
    21061 gaaataaccc actgataggc actgaccgcg ggggatttgc gaagcagaaa tcctccgtgg
    21121 tgctgtgcct gttcatggac ggcggcagcc ggccatgatg tctcaccccc cgtatctaac
    21181 aggggggtac aaaaacacaa aaaacaagca agtaaccata aaaaatgctt gttgtatcaa
    21241 ggatattaag taatttgtat aggtgttaac tcatttggaa aagttaacac cttgaatttg
    21301 gaggtgaaaa cttggcgcaa cgaaatttag attatcggtt attaaaagaa cgacgcaacg
    21361 aatatggcgt ttcacaaaat aaattagcta ctgcttgcgg acttagccgc ccatacctaa
    21421 atcagataga aaacggtggc gtaatcgcct ctacaaagac catgagaaaa atttttgatc
    21481 aactggaaag tttcaatccc gatttacctt taacgttact atttgactat gtaaggattc
    21541 gctttcccac aacggatgtg cggaaaatta ttcaagaaat tctccatttg aaattcgagt
    21601 atatgcttca tgaagattac gccttttact cctatcaaga acaatatgtc atgggcgata
    21661 ttgtagtgat gttatctcat gaagaagata aaggtgtcct tttggaatta aaaggtcgtg
    21721 gttgccggca gtttgaaact tttttactcg cccaaaagcg tagctggtac gacttttttg
    21781 aagattgtct aaaagccggt ggcgtgatga aacgcttgga tttagcaatc aatgatcgag
    21841 tggggctact ggatattcct gatttaacga aaaaatgtca gaaggaagaa tgtatctcct
    21901 tgtttcgtac cttcaaaagt taccgttccg gggaactgtt gaaagctgat gaaaaggatg
    21961 gtatgggcaa taccttatat attggcagcc tcaaaagcga ggtttatttt tgtttgtacg
    22021 aaaaagatta tgaacaatat atcaagttag ggattccatt ggaccagacc gaaacgaaaa
    22081 atcgttttga gattcggttg aaaaatgacc gtgcttatta tgcgattcaa gatttgttaa
    22141 aaggtcgtag cattgaaagt accacgtttt ccattatcaa tcggtatttg cgatttgcgg
    22201 ataaagtaga tggaaaacgg cgaaccaatt ggcccttgaa tgaacaatgg ggtcgtttta
    22261 ttggacggaa tcggaaagag attcaattaa cttctgaacc gaaaccgtat accattgaac
    22321 gaacattaaa ttggctaggt agacaagtcg cccctacgtg gaaaatggct aaggagttgg
    22381 atcgattgaa tcaaacaacg tatatccagg atatggtaca gaatgcgcga ctctcggacc
    22441 ggcataaaaa gattttggag caacaaagta tggcaatcga aaacttgatt gtatgaaaag
    22501 aggaattttg atggaaattg taaaagatat gttattggta cttggtggca gtatgcttgg
    22561 tgttaccgtg atgtgcttga tgaatgcaag tgcagcagcg gattgagcaa tggaaaagaa
    22621 aggaaagtga caaaatgaat ttcggacaaa atttatgtgc gactcgttcc ttagtgaaaa
    22681 gctaaggcac taataaaaga acgaaaaagt ttggttatta ggagaactga taatctgaca
    22741 ggtggaatga tgaagtaacg ctctgaaacg cctgccctga gcctccgaca agcattgtat
    22801 gaggaaactc agaatgtttg aagctcggta aagacggctg aaagataccg taatagttga
    22861 tgaacatata tcttctatcg aaaactatcc agaggtgggt agcgtatcca acaggtcggg
    22921 ggtctataaa atatctatgg ttagaatgtt ctataaggaa ctgacgaaac agcgaatgta
    22981 cgggtctaaa tttgaacttt gtagaaatgc aaaggtacga gaaatcgtag ctggtgtggc
    23041 aaagtaagaa tataggttat gaaatgccat ataacgttac aggcggtatc aagccagcag
    23101 gcttatagct agtgacctaa ggatatatat gtatagataa gattatcgga acgaggaaag
    23161 gtattgggtt ctcatcaaga gaataaactg acgaagaaca ataagcagtt gaactaatgc
    23221 tgaaaagcag agggcgaacc taagaaattc ttgtaatgag aatggaggaa tgccctcaag
    23281 tcgtgtatat attagaaaag tattatgttg tttcaataag gattacgagt aagataaaaa
    23341 agctcactca atttcaagta aaggagtgaa gccttatgcc taaaaataaa aatgaaacat
    23401 tgtgtgtaga agatttaaga cacgctgaat attacgaaat gcaaaacacc tttgatgatt
    23461 tatatgccaa aagtaagaat ggagatattt tcactcatct tatggatatt atcctatcga
    23521 gagaaaatat attactggca tatcgaaata tcaaagcaaa tgcaggcagt aaaacagcag
    23581 gaacagacgg tacaataatt aaagatattg gcaaattgcc agcagaaaca gttgtcaaaa
    23641 aagtaagata cattgttgca ggcagtccac atgggtatcg acctaagcca gtgaggcgaa
    23701 aagaaatacc aaaaccaaat ggtaaaacca gacctctagg tattccttgt atgtgggaca
    23761 ggcttataca acaatgtata aaacaagtat tagaaccaat ctgcgaagct aaatttagtg
    23821 aaaacagcta tggttttaga cctaataggt cagttgaaaa tgcaataaaa gcaacctata
    23881 accgcctaca aatcagccag ctacattatg tgattgaatt tgatattaag ggtttctttg
    23941 acaacgtgaa ccattccaag ctgataaaac agatatgggc tatgggaata cgggacaagc
    24001 acttgatatt cattttaaaa aggatattaa aagctccaat caaaatgact aacggtacaa
    24061 taacatatcc agagaaagga actccgcaag ggggaataat atccccacta ctagcaaaca
    24121 ttgttcttaa tgagttagac cattgggtag aaagccaatg gcaggaaaat cctgttacaa
    24181 aaaactatgt ggttcatatc aataaaagcg gtagcccatg taaaagcaac gcttacaaag
    24241 aaatgaagaa aacaaagtta aaggaaatgt atatggttcg ttatgcagat gattttagag
    24301 ttttctgccg ctacaaggaa agtgctgaaa aagccaaaat agcaatcact caatggattg
    24361 aacaacgatt aaaattggaa gtatcacaag aaaaaacaag aattgtaaat gttagaaaaa
    24421 gatactccga ctttcttggt ttcaagatta aaatgatacc gagaagaaag aagttagttg
    24481 ttaaatcaca tataagtgat aaacaattca aaaaccaaaa agacaagctg gtatcacagg
    24541 caaagaaaat tgcttctccc tcaaaaggga aaacggaatt agaagaaaca agactttata
    24601 attcaatggt attaggtatg caaaattatt atcagattgc aacgaatgta aatctggatt
    24661 gtatgaaact gaatagagct gtaatggcta tcttcactaa cagattaggt acaaacaaaa
    24721 ggggcagatt gagaaaaaca ggcagacaac ttacgacaaa ggaggcaaca agatatggta
    24781 aatctaaaat gcttcggtat gttgctggcg agggcgaacc tattttccct gttggatata
    24841 tccaacacag aaaaccaatg gcaaaggtgt atttagcgaa ttgctacact ctagagggaa
    24901 gaaaatttat ccacaccaat ctaagtgttg ataaattact gatgtaccag ttaatgaaat
    24961 tatctttgga aaatcgcaca atagaatacg cagacaatcg aatttcgcta ttttctgctc
    25021 aacatggaaa atgtgctata actctggaag aatttcaaca agctaatgaa atacattgtc
    25081 atcatattgt accaactgga gctggtggaa atgacgatta taaaaatctt attttggtaa
    25141 aagaggcggt acaccgtcta atacacgcta aaacagaaga aacaatacaa aaatatatag
    25201 ctctattgaa gctaaataaa gtccagctca taagactaaa taaatataga gtattagctg
    25261 gcaatcaaga attaaacttg atataggtct actacataaa tacttttcaa tataaacacg
    25321 atggaacgcg gagtgcggtg aaagtcgcac gctccgtgtg aatgggggga aaagtttgag
    25381 atatttataa tatcaggaac ttacctatcc atatatcaat ggtttttaac caatgctcaa
    25441 tctttagtct tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgggagttt
    25501 tccaaactga ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct
    25561 tctggtgtga aggatgtcct actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga
    25621 atcacaatgg aacagatgcg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag
    25681 ttggtggggg catggtttac cccacctgtc gattttgatg aagtgaaaga acagattgga
    25741 ttaaatgacg aatatgaaga gtacgcgatt catgattatg aactgccttt tgagattgat
    25801 gagtatacgc caattgaaga gattaatcgg ttgtgtaact tagcggagga actagaagga
    25861 acgccaattg gtgaagctgc ttctgagatt caacacgctt tcttcaattc tttcgaggaa
    25921 atggtagagc atgtggatga tattgtctat tatccagatt gcgatgatat gagtgatgtg
    25981 gcgcattact tgattgtaga agctggtgat tttggcgaag ttccagaaca attgaagccg
    26041 tatattgatt ttgatgcttg cggtcgagat ttggagattg gaggaaatta tttggttact
    26101 agtcgtggag tttttgagta tccagaatga atcaaacact atatatagtg tttgattagt
    26161 atatgtaagc aaaaaaacac aatatataga gtgcaaactg aagggtaatg tgatataata
    26221 tttgccgaag aaatattttt gaaatgaggt aatattatgg caaatattca aacaagtttt
    26281 attgatttcc ataattcaat tagattggac gtagaagaca atacgctact taaggactat
    26341 aaagaccaag taattgatgg attaaaagat tacttacctg atgatgtgaa gtttgaaact
    26401 tttttacagg gaagctattc tgtttatacc ggaataaaaa gctgtgatga aaaaattgat
    26461 tttgacatcg atattgcagt tgctttcgaa attgatcata ctgtttatga agatccaagg
    26521 gagcctaaac tgtgggtaaa agaggcgcta gttgaaattt ttcctaatgc tcaagttaat
    26581 ttaaaagtac cttgtgtaac tgctacattt actggaaaaa agacaaaaaa aaatgtgcac
    26641 gttgatgttg cggtttatgc taaagaagat gaaaattatt tcttagcaaa agcaaaagaa
    26701 ttcagtgctc ccgaaaaccg atgctgggag gaagcggatc ccaaagtatt aaaagagaaa
    26761 attaatagtc atgtagcaga ttcagatgat cgaaaacaat ttcgaagatg tattcgctat
    26821 ttaaaaagat ggaaagataa caattttaac caagaatata aaccaacagg aattggactt
    26881 acaattaatg tgatggatac gttcctagtt aataagtcca ctgatttttt aactagaaaa
    26941 gttcagtata atgatatgga atgtatgaag cagattgttt cttcgctgaa agattcattt
    27001 gtgtatgagt acagtgaaac agatggttgg cattatcgtc tacatgcata tcttccagtg
    27061 aagcctaaca gtgacaccta ttcaaaaatg acagtgaatc agatgagtga ttttaaaaac
    27121 aaactttcta aattatatga tgatttgatt ttcgctatcg atactgaaga tgagtatgaa
    27181 gctacaaaac gattaaataa tcaatttggg gaggattttt taattatttc agaagaagaa
    27241 gttacagaaa aaaacttaag aaatgcattt gttacagact atccgagtgc ttaggttggt
    27301 gttaaagatt ggattatact gatattttaa aagaaaagct aacagatgtt gtgcgtttat
    27361 tcgatggtaa ttactatggg gatttttcat ttgctacaat tggaaagttg cgtttaaaaa
    27421 ttgaaatacc aaaacatttt ccattggtac ttcctaggat ttacattcat aattttgatc
    27481 atttaggact ttacctacca catgtagaaa aagatgggtt ggtatgtttt tcttcagata
    27541 taaattattt agtagatgaa gaaaattcag aattggtttt attgcagtgt gttaataaaa
    27601 ctataaaaac aattgaggaa ggtttgaaag ggttaaataa aaatgatttt cgaaaagagt
    27661 ttgttgcttt ttgggacaaa caagagaatc aaatttcgtt attctacctt tgtgaaccag
    27721 cgttgtttat caagtcagtt gatacaataa tgtacggtaa gtctttggtt gctttggata
    27781 aagagaacga attacaatta aatgcagcga gtagattgtt taatggagag aaagaagata
    27841 ctcatgaatt agatgcatac tatgttccaa ttagaaacaa gaataatatt atgcccccga
    27901 agaataataa agcatggtca aagaaggata ttttacaaat agtaaaaaaa aatgtaacag
    27961 gtgcaaataa aagatttttt ttaaaacttg aaaaaagaaa gaatagacat aatgttttac
    28021 ttattctaga tatcccaatt gaagaaaagt caaataatgt cataattgga tttcttttta
    28081 caaaacaatc tggatctata aaagattata agggtacgcc taggactgta ttagttcagc
    28141 gtcatgatga attatatttg gtgaatagaa cgagcggaga aaactatgga ttgggtgcta
    28201 atatagctat tgttggggta gggtcagttg gcagtaaaat tgctgaaaat cttggtccat
    28261 taggtgtgag aaagatgaca ctcatagaca atgattgttt tcaagctgaa aatttgtatc
    28321 gaaattcact tggtgcagat agtttaaaaa atgcccgatc tcagaataat ttttttaaag
    28381 ttgatttaat gcaggaggag ctcgaaaaaa aattcccata cctttgtgtt gaagtggagt
    28441 caatgaacgt tttagactta attagtatgc aacctggttt tttcaaggaa tatgactatg
    28501 tttttgtttg cataggagat acaatggcaa gcctggcttt aaataattat ttttccaaaa
    28561 gtagtcaaaa agttttctat tcttggctgg agccatatgg cattggggga cacactctat
    28621 ttataaatta ttctctaaat caaaaaggtt gtttcaattg tctttataca gattcaaact
    28681 ctaaaaatag gtgttcaaat agagcttctc tggtaaaaga aggacaaatt tttgaaagga
    28741 ctttagcgag ttgccgctca aaatttatac catataatgc tattgcctcg acggaatgtg
    28801 caattaatgc aataaaactt ttccataaag tcaatcaaaa taatatgacc aatagtactc
    28861 ttttttcatg gataggagat gggagtactt ttgagaattt aggatatcaa tatagcgaca
    28921 gaaatacttt tcttaatgga gtcaataagt ttgaagaaag attcttttct aaatattgca
    28981 aagtatgtgg ggagttagat gattaaattt tcgtgtgacc gtattgtcat tgaagtaagg
    29041 gatgaagttc ttaagatatt tgaacaatat cggcagggaa aggatgactt gcctgaagct
    29101 ggaggagttc ttcatggaaa aagacttaag aatgggagca taataattac cgatataaca
    29161 acacctcaaa taggagatat acaaaaaaga ttactcttta aaaaaaaccg agaaattcat
    29221 caacagcttt ctaatgagaa atggatagaa tcaaataagt tttctattgc attgggagaa
    29281 tggcatactc atcctgaagc tgtaccaact ccatcaaatg ttgataagaa aagctggaga
    29341 ctgaacgtga gcaagcaaca tgatgataga gtttatgttt ttattatcgt tggattttct
    29401 gatttgagaa tgtggttact gtcaaaaaaa tcagatatta ggcaggctat aaaactataa
    29461 aggagtgatt aggtgaaatc acaaaagaat atttggtggt gggggttttt agtaggaaag
    29521 gtaatgatac cagcccttct cttaatttct ctaccgatga ttactttttt ctttaccaat
    29581 aaaaaaatga gtaaagaagc cgtgattttt ttctttggag accaaaaagc tacctttatt
    29641 gaaacgttag taacatctct tcagcttaat ttgaattaca ttttttcaat tattattgtt
    29701 atatcgttat tgaatttttt taaaaaaaga aattcaggaa aggtgtttaa ctctaatgga
    29761 aatgtgtact acaactattt ttattttgta ttttgggtag cagcaacttt acttggttat
    29821 gataaaattc agattgcagg aattcctata cacatgcaat ataagttggt tttgagtgga
    29881 attttttctg aggtgttgcc agatatatac gatgatcatt acgacagtga tggaacatgt
    29941 aaagtaagca ttgaaaaaga aaatttcgat gacatcgatg gatatgactc tgtgaactta
    30001 ctgattattg atacttacga tataaaaatg agtgaattat ctatggaaaa tcaaacatat
    30061 cctacgataa ttgttagagg gaattcaata gatggagttc gtaaagttaa tagatcttta
    30121 atcttggaaa taaaaaaaac aatggatgaa attcaaaaaa gtgactttaa aaaagtattt
    30181 gttgcttcaa catctaatcc aaaaaatagt atcaatatta ttaattcatc gtttagattt
    30241 ttcggtcgat cacgtaggtt taaactttat gttcttcaaa aggattatgc aagcaatgga
    30301 aagtatagta agaaatatag aatttttatt taagaaggaa tttttccttc tttttatttt
    30361 ggaggaaact atgaaaaaaa taaaaagcta caccagtatt tgggcagttg aaaaagtcat
    30421 ttatgcgatt aatgactttc agctgccttt tcctgtgacc tttaaccaga tggcttggtt
    30481 tgttttatcc ttgatgtttg tcattgtatt tgcccatgtg cctccgctat ccatgataga
    30541 gggggctttt ttaaaatact tgggcattcc gatagcggtc acttggttca tgtcgcaaaa
    30601 gacctttgac ggtaagaaac cattgggatt tttacgatcc tttatcagct atcacttacg
    30661 gtttaaggtc acttttgcag gaaagaaagt taaagaacag aagaaacgat gggacgaacc
    30721 aatcaccttg gtaaggagtg tgaattatgt acccgattaa gtatattgaa aataatcttg
    30781 tctttaacca agaaggcgag tgttttgctt actatgaatt agtgccttac aattactcct
    30841 ttttgtcacc tgaacagaag tatcaagtac atgataactt tcgccaatta attgcgcaaa
    30901 atcgtgaagg taagatccat gccttacaaa ttgccacaga aagtagtatt cgtgccacac
    30961 aagaacgttc gaaaaaggaa attaccggta gactcaaaga agtagcaaag caacgaattg
    31021 atttacagac agatgcatta gtatccatga ttggcgatag tcagattgat tatcgcttct
    31081 ttattggctt taaactgatt tcgacagatg aagaggtcaa tctaaagaat ctcaggaaat
    31141 ctttcttttc tggacttcaa gaatttgtct atggagtgaa tcatcatttg atgggtgatt
    31201 ttgtttcctt atccaatgaa gaaattcgtc gctataccaa gcttgaaaaa ctaatggaaa
    31261 gtaaattggc tcgaagattt aaagtcaaac gagtaacgcc gagtgacttg acgtatttaa
    31321 ttgaacatat ctatggtgaa aaaggcacac cgtttgaaga atatgaattc cagctaccaa
    31381 agaaaaagct gaaatcagaa acgctggtga aacgttacga cttattacgt cccactcgtt
    31441 gtttgattga agaaaagcct cggtacttac ggatggaaca tgaaaatcat gagtcttatg
    31501 tagcgtactt aacaattaat accattgtag gggaaatgga gtttccttcc tcggaacttt
    31561 tctattacca gcaacaacaa tttacgtttc cgattgatac ttcaatgaat gtggaaattg
    31621 tgacgaataa aaaggcccta gctaccgttc gtaataagaa aaaggaatta aaagatttag
    31681 acaaccatgc ctatcaatct gataatgaaa cgaactccaa tgtattagat gctttggatt
    31741 ccgtggatga attggaaacc accttggatc agtcgaaaga atccatgtat aaactaagct
    31801 atgtggttcg agtgagtgct aaatcagtgg atgaattaaa acgccgctgt gatgaagtgc
    31861 tcgattttta tgacgatacc aatgtaaaac tagtccggcc ctttggcgat atgatgggtc
    31921 tacacaaaga attcttaccc tcaagtaagc gatacatgaa tgactatatt caatatgtta
    31981 cgtctgactt tttggcggga cttggttttg gggctactca aatgctagga gaattagaag
    32041 gcatttactt tggctataac gtagatacgg ggcgcaatgt ttacctgaaa ccggcattgg
    32101 catcacaagg ggtcaaaggc tcagtaacca atgctttggc atctgctttc cttggttcgt
    32161 taggtggggg gaaatctttt agcaacaatc tcttagtcta ttatgccgtc ctttttggtg
    32221 ggcaagctgt gattgtcgat ccaaaaggag aaagaggcgg ttggaaagaa acgttgccag
    32281 aaattgcgga tgaaattaat attcttaact tgaccagtaa gccagaaaat caaggcttac
    32341 ttgatcccta tgtgattatg aagaaaaaga aagactcaga aagtttggcg attgatatcc
    32401 tgaccttttt aacagggatt tccagtcggg atggggagaa gttccctgtg ttacgcaaag
    32461 ccattcgaac ggttacccaa agtgatcagc gaggattatt acaagtaatc gttgaattgc
    32521 gtaaagagga ctcaccagtc gcggaaaata tcgcggatca catcgagtcc tttaccgatt
    32581 atgattttgc ccatttgctt ttctcagatg gttcgattac ccagtcgatt agtttagata
    32641 agcagttaaa tatcattcaa gtagcggatt tggttttacc agatgcagaa accacttttg
    32701 aagaatatac caccatggaa ttgctgagtg tttccatgct aattgttatt tccacttttg
    32761 ccttggactt tatccatagc aaccgagaaa ttttcaagat tgtggacctg gatgaggctt
    32821 ggagttttct tcaagtagct caaggaaaaa cgttatcgaa taaattagtg cgtgctggtc
    32881 gggccatgaa tgccggtgtt tacttcgtca cgcaaaacag tgatgacttg ttggatgaac
    32941 gtttgaagaa taatattggg ctaaagtttg catttcgcag taccgatatt catgaaatca
    33001 agaaaacctt ggaattcttt ggattagata aagaagatga aagtaatcaa aaatgcatgc
    33061 gagatttgga aaatgggcaa tgcttgatgc aggatttgta tggtcgagta ggtgtggtcc
    33121 aagttcatcc agtctttgaa gaacttttcc atgcttttga tacccgtccg ccagttcaag
    33181 agagaatgga gtgaggagat gaaaaagaaa ctttttcgta tctttggaat tgttttaatc
    33241 agtttggtca ctctgataat acttttgagc ttagttggaa cggtagctga ggcaacaggt
    33301 ctggttgatg atacagttgg agccggaaat ctttattccc aatattcact tggtaactat
    33361 cagttggatt tctttgtgga tagttcgtgg gattggctgc cttggaattg gggtgatggc
    33421 ctagggaaga gcgtaatgta tggactttat gccattacta attttatttg gacagttagt
    33481 ctctacttat caaatgccac gggctacgtg gttcaagaag cctataagct ggatttcatt
    33541 tcagatacgg ctgaaagtat tgggaagaat atccaaacct tagccggcat taccgaaaat
    33601 ggactccaga cctcgggctt ttactttggg tttctattat tgatgatttt agctctagga
    33661 gtttatgtgg cgtatacagg attattaaaa cgagaaacta ccaaagcagt ccgggctgtc
    33721 attaattttg tgatgatttt tctactttcg ggttcgttca ttgcgtatgc gcccacctat
    33781 atcacaaaaa tcaatgattt tagctcagat gtcagcgaag cggctcttag ccttggaacc
    33841 gagattgtcg tgccgaattc agaaagccaa ggaaaggaca gtgtggattt aatccgagat
    33901 agcttgtttt ccattcaagt ccaacaaccg tggttactct tgcaatttga tgattccaat
    33961 atagaagaag ttggcgaaga tcgtgttaac aaaatcctgt ctgtaagtcc agatgaaaat
    34021 aaaggcaagg atcgggaaga agcggtcaaa gcggagattg aagataatga caatgcgaat
    34081 ctcagtatta ccaagaccat gaaccgctta gggaccgtgg tctttttggt gttgttcaat
    34141 attggcatat ccttctttgt ctttctccta acagggatta tgttgttctc gcaaattctc
    34201 tttattatct ttgcgatgtt tttgccgatt agtttcttat tgtccatgtt gccgacttat
    34261 gagagtttgg gcaagaaggc gattattcgc ttgttcaata cgattatgat gcgagcagga
    34321 gttactttgg tgattacgac tgcatttagc atctccacga tgtttttcaa tatttcggca
    34381 acctatccat ttttcatggt tgcttttctg caaattgtga cctttgccgg tatctatttc
    34441 aaattgggcg acattatgaa tatgttcaat ctccagagta atgatagcca atcaatggga
    34501 agacgggtca tgcgaaaacc gcaaatgtta atgaatcgaa cattgcggca actcaatcga
    34561 aatgtgggac ggactttggc ttttggtggt ggtgcagcag ttggaaataa gttggcaaag
    34621 gaacaatcaa aatccaaatt taagccatcc ggttcttctc tacgaaagaa ttcacgatta
    34681 ccgaacgatc acgagatacc atctgacttg aataaggaaa agccaattcc aaataataag
    34741 aaacaatccc gaatgaattt ggccgggaga aaagtcggca aggttctgga tacccaagct
    34801 ttggtgaaag ataagaccaa acaagtaaag gatggagtac ggaatacgcc aaccaacttg
    34861 aaatacaact tccataaagg aattgaaaaa acgaaaaaag caccggaaga gtttaagcga
    34921 ggactggtcc aagaaaaagc aaatcgagga gagttgcgag aaaaacaaca acaacgtaga
    34981 gacgaaaaaa tagctgaaaa gcgcaaagta ttgaacgaaa tagggaatcc tcatgggaaa
    35041 agaagggaaa atgttccgat aaaagcaaag gtccaatctc aaaaggatag agcaccaaag
    35101 cggattgtga gagcaaattc gaatcccgaa atcaaacgaa aattcgctag ccaagaaatc
    35161 atttcaaaag gaataaacca atcctcaagt aagaaaaatt cttttcaaca agtgaaacgg
    35221 cggtctactc tgtccaaacg aaccaatcga aaagtacaaa aaagtttgaa ccctcatccc
    35281 aaaccaaggt caggtgagaa aaaatgaacg taaagaaatt aagtgccatt tcgttaactt
    35341 tcctaacaat ctcttttatg ggtatcttga tgtgtgtggg cttattgttt ggtgaagaca
    35401 gtgaaggaag tagtagttca ggtgctcctc ctggggggag tagtgtctca gaagaggttt
    35461 tgaaacaccg tgcaatggtg gaaaaatatg ccaaagaatc ggatataaca gagtatgttc
    35521 cgattctttt agcgattatt caagtggaat ctggtggcac aatggaagat gtgatgcaaa
    35581 gttctgagtc agccggttta ccaccgaatt cattaagtac cgaggcatcc atcaaacaag
    35641 gatgtctcta ttttgcgtct ttggttaagc gttccaaaga attaggttgt gatcaagata
    35701 gtatcattca ggcctataac tttggcggtg gttatttgga ctatgtggca aaaaacggga
    35761 agaagcatag ttttgcttta gctgaatctt tttcaaagga aaaatcttgc ggtcaaaaaa
    35821 tcgattaccc taatcccatc gcgaataagg aaaatggtgg atggcgctat aactatggca
    35881 atatgtttta ttgcttattg gtgaagcaat acctaactac aacacagttt gacgataaaa
    35941 cagtccaagg gatttttgac gaggcgttta agtatgaagg aacggcttat gtatttggtg
    36001 ggtcaagacc cgaaacagga tttgattgta gtggcttgac tcaatggtgt tatgccaaag
    36061 ttggtctgaa acttcctcgg gtggctcaag atcagtatga tgccatgacc catattgacc
    36121 taaaagatgc caaaccaggg gatttggttt tctttcattc gacttatgat gccggtactt
    36181 atgtgaccca tgtcggtatt tatgcagggg agaatcgcat gtttcatgca ggagatccag
    36241 taggctggac gaatctaaca gaaagctatt ggcagcagca tcttatcggt gctggacggt
    36301 acaaataaga gaaaagagga aaaagaatga agataaaaat tgaacggaat caaaaggaaa
    36361 agagcctaaa aaagaaaaaa gaacgagtcg tatccgtggg taagcatcga aaaatggtgc
    36421 ttgccctttg gcttttatta tcttgtagtc ttacttttgg cgtttataaa aatttcaccg
    36481 ccattgatca acatacaact catgaaaaag tggttattaa agagaaagta gtgaatactt
    36541 ctggtattga aaactttacg aaggattttt cgaaagaata tttttcgtgg aaaaataaca
    36601 aagaagcaat tgaaaagaga atgaacaacc tcgaacagta ccttacagaa gaaggtttcg
    36661 ctttaagtca agatatggtt cgagcagata ttcccactaa ttccgaagtt cagtcagtaa
    36721 aagtcttaga tgttgaaaag agctcggagg aatttgtcgt ttcattttta gtggagcaaa
    36781 agattacaga gggaaaaaag gcacaatcga tttcttctgc ataccgagtg acgatctttg
    36841 aagatgaaaa caggaattat atagtaacga gtttgccaac tatgattgca aaacctgata
    36901 gggcaaaata caagacaaaa caagtagaaa atgattcgaa aattgatgcc aaaactacag
    36961 aagaaatcgc tgagtttttg gaaacgttct ttaaattata tccatcagct tctgaaaaag
    37021 aattggagta ttatgttgaa aatgatgcga tgcgaccaat caatattgac ttaaagtttt
    37081 cgaagttaga ggatccagtt tacctacaaa acggaaaaaa aattctagtg agtacaaaga
    37141 taaaatacct tgataatgta tcaaaaacca ctagctactt tcagtacaaa ttaatattac
    37201 aaaaaaatgg aaactggaaa atcattgatg ttaaataata atgactcaat ctaaatttat
    37261 gagaagttat aagtagttta acttatttca ggcagaagtc gcccatcctc tataggtggg
    37321 tgatgaatgc cgttcggtaa ctcgaaaacc gacaattaag acatagtgcc tttttatttt
    37381 aatccatgtc ataatcagtc tatataagaa atggttgata taccatggaa ttagatacaa
    37441 ataatcattc attatttctt ctttgttacc accttatttt agtagtgaaa cattgtaaac
    37501 atctcattga tgatgtaatg ttagcttatg caaaaaacta tctttgaaag aatatcagaa
    37561 tcacatcaca ttaccttagt tgagtgaaat cgtgataaag actactttca tattatgctc
    37621 aaagcacaac ctaaaaccga attaacaaaa ttcagcaatg cctaagctca tgtctgataa
    37681 aatgagactt tctaagggtc aaaaatttcc tttgaatacg atcattagct tagataaaag
    37741 taaaaacctt agaactacgg ggatagcttg gttaataaga gatatcgctg ttaataaaga
    37801 cataagcttg caagtatgct ctattcccaa gaatctccca cttcaagcac taaggatcgt
    37861 taaattcagc taagtggcaa gtagttcact agacgataat tatctccata tttcaatctc
    37921 agcttttcca tttttcatag caaattccgt tatttttata gcatctaaaa gattcagctt
    37981 ttccactgat ctttcaccac ttttcagctt ttttattgtg cttaaattga taccagtacc
    38041 ttttgatatt gaattggaag taaaatcatt aagggttttt tttattaaat tcatatccgc
    38101 aattccagcg ttcataagaa tgaagattct cctctcatag actactttga aaatggtact
    38161 acagtggctg tttatatatt tcagtaggtt actgtagtgg tcattttatt ataattagaa
    38221 aaggaaccta aatcaaaaat gctaaagaaa attttcatag tattaaacct atcatgacta
    38281 aagccacgga attcttgata aaagagtata cttgcaagcc tatgtcttta ctaacagcga
    38341 tatctcttat taaccaatct atccccgttg ttcctaaggt tcttgatgtt ttctaaggca
    38401 atgcttgtat acatagccct tgatcaaaat attgatacta acgttgacgt ttcgatcatg
    38461 atgtgtacgg caaataggac aagtccattc tcgaattgtg aatgacttct taccatctgt
    38521 gtgtccacat tctgaaccaa tttgactaga cggaaatcat ttatctatct tgatgatttt
    38581 tttgacagaa gtatcgaaaa tctttacata atctgggtgt tgcattctta tgcagatgag
    38641 agcgttagct ccgtattcat aaccagttgc ctctaaaacg tagtccatcg gtggatacct
    38701 agcttgcggt tgccagtggc tttcgcagtc ttcttgtaat caaatcgagc atctaattga
    38761 gtactagtat tttgaagata atacttgagt ttttcggttt tcagaagttc acttcatctt
    38821 ccatcatcca tggaaatccc actcacccat acgcaggcct ccaaaaattc ttcaacaatg
    38881 gtttttcaga catttttctg aatacctcct tgttttttga tacgccagat agtcaggaaa
    38941 aaggcaacaa aacacaaaac aaagctgcct aaaaaggtaa tcttcattcc atataaaaag
    39001 acatccggcc gatctgcgat atagttagtt actcgttggc ccatcttagc agacatcgct
    39061 tgatataaga ccgtcgtaga aagcgaaatc ccgatgacca tccccaaatt tcgggcaaag
    39121 gaattcatgg agcctgccag tcccaaattt tcgatagtga cagaactcat aaccatcgta
    39181 ttatttgggg actggaacaa ggcattgccg gcacccatga tggcagtagc gacaatgtac
    39241 agccacaaag gagagtccac tgacaaaaac atatacatga cttgggttac cgacaataaa
    39301 cccaagccta ctaatacaaa aatctccggg ccaacccggt cggtcaggta tccgcttaaa
    39361 ggcgccccga cgaccatcat caagggaaag accatcatca acagtccggc atagctagga
    39421 ctcaacccgc gagcattttg cagataaaaa ggaatcacta cattaacaaa gaagttggaa
    39481 gaaaaaatca acaaggctgt aatcaagctc atggtaaaaa tcttgttctt aaaaataccg
    39541 aaagaaatca atggagaggc tgctttcttt tcaactttta aaaagctgac aaaggcaatc
    39601 actgaaatta cgaacagtac aagggggact ggatggaaaa agccactttc ttgccccaaa
    39661 aagattgttc caaaaaggct gataatcgta gccgcaatgg cagcaaagcc ccctccatcg
    39721 attgtatggc cgctcatggt gatatctttt ggtaagaatt tttcagcaat caacatcgtt
    39781 acgattccca ccggtacatt gatccaaaaa atgtacgccc aagaaaattg ggacaaaatc
    39841 aagccgccaa gacctggtcc agcaatcgag cctaatgaaa caagcgcacc gattgctccc
    39901 aatgcccgac cacgttcatg tatgggaaag acttccgtga tgattccggc atttgtagcc
    39961 atagtcatac tagcaccaat ggcttgtacc acccgtgcga acaattaaat acctagcgaa
    40021 tgattgaacc cacacaacag cgagcccaag acaaagatca gcgtaccaat ccgaaatact
    40081 ttgattttcc cacagctatc gccaatttta ccaaacagca acaaacaaga acagacaatc
    40141 atcagataaa cggacactac ccattccgct tgattcattg gtatgcccat atcatctgag
    40201 atggtaggta gtgcaatatt gacaatactg ccatccaatg tggacatgaa ggtaaacata
    40261 gatatagcaa tcaaaatcca ccagcgattt ttttggactt ttgcatcttc ttgataacta
    40321 tgcataatag gatttccttt ctaatgatcg tgtagctaag aacaatataa cctatgtcta
    40381 ctgcccaaca acttctcgcg actctagcca taggaagctt gagtaatttc gatttgttat
    40441 tctcatgtaa ccatgtaagt ttgagatgct ttatttgaac ttactctata gtaactagtg
    40501 ataattcgct ttactttctt ctagcaattt ctaaaagtcg acttcattgt tcttcagatt
    40561 aatagtgtga gctatctcgt gagacgtaag aaaatttcca ttctactttt tcccaatttt
    40621 ttcatattta tggtgttgca cctatttgtt ttccaatttg cttttcaaat acattgctat
    40681 gatattcaat tccataggat tatactaaat gtcaatagta tgaataatat attatagatg
    40741 tttatatatc actgttaatt attatccaca ataattaatg attacctcga tatttgtcaa
    40801 tcaaaggttg aaacaattta ttttcagcat cttttctaac ttgagctgca ctcttaatag
    40861 aatcatacga tcctaaatag tacctctttt ttttaaagtt atatacgcaa tccatttctc
    40921 tttagaacga taaactccct taacaccact tgtattattt ttcggtaatt ttgacgttag
    40981 acactctatt actgtccctt caacacatgt ccttgtttct tgccttttta aacatccaca
    41041 actttgagta tgtccaaaaa ataagttact ataacttatc tcacacatac tgccacactc
    41101 acattgacaa acccattttg ttttccatga acagttattg tccaaaggat actttgcagt
    41161 aagtaatccg aattttttat tttcaatgtg atgctttttt gatttcaaac atccacagct
    41221 ttttacatca ccttttagaa gtcgctttgc cgatactaac ttttttctcc cacacagaca
    41281 ttgacattcc caaacaacag ttccttcact tcgcagttta gttggcttga ttgcaactaa
    41341 cttaccaaaa gttttatttg tcaaattttt atatttatgc ctatttttat aatttccaca
    41401 tccacaactt ttggttctac ctgatagtaa gtttgatcga tatacaatcg tttcattccc
    41461 acagtcacat aggtaacgat attttttata tccattatga gtaatctcct caacaacagt
    41521 aaggtgacca tatctttcct tcactgcacc taccctccca agaaagaagt accatataat
    41581 ttcaattaac ttttaataaa tcagaaaaaa atattttcaa tttttcttag tctgatttta
    41641 tgactaattt ataatacaag actgaacgat acgtacacga aatcctcaag atagataaat
    41701 tgttttcatc tagtaaaacc tgctcaaaat atgagcataa agacggcaat aatacactcg
    41761 atattcgata atggacttgt tttatttgtc aaactcatcg taatcgagat atcaattcca
    41821 gtctcaatat cctgatcaaa ggtctaagaa tccaaccatt ttcttgttac tttgtcagat
    41881 tgattacctt ttttagtctc cgaataataa ggttttcgga tcaactaaca tgctctgaga
    41941 aagactacta tctttgcaaa tttccaaatg aaggtgtggg ccagtactat tcccagtact
    42001 tcctacatag ccaattattt gtccttgcaa aacattatct ccagttttta ctaggtattg
    42061 ttgctggtga gcatataacg cggttgatcc atcttcgtgt tgtatcacca cgtaatttcc
    42121 ccaagagtag tgaaactctg ctttaacaac cttaccagct ttaactgcat gaatcggctc
    42181 accctgtgta gctgccatat caagtcctcg gtgaaattca tttccacgat ttccaaacaa
    42241 actggttatt gaataattat gaataggcac ttggtatcca gtcttactca tttgtggacc
    42301 tttaacttct ttatcatagt tggttaaatc ataggtttta attaattcat ttagcttcaa
    42361 atagtattgt gtatctgttg cataccgtcc tgtaaggaat ttagttgctt ctcgatatgt
    42421 tttactatta gcttttaatg ctcctgcata aaaatcttta tttccactaa ttcccttagt
    42481 taataatttg gcataatctt caaaactttc ctcatagttt tcatacactc taaattttga
    42541 ttgggtcgta taaagcgttc cattacccaa atcttcgttt gttaccataa tgacaaagtt
    42601 tccattataa gttcctttta tcccaaataa attgtaattc ggtgcctgag ctaactggct
    42661 ttgaccactg gcagattcta agatcgcttg tgcaatcata acagaagcat ataaatcata
    42721 tttctgacca atttttctcg cagactcacc aattttcata ataaaatttt ctacagattc
    42781 atttttgtca aaatgaattt cttcattacc agatagtatt ggttgtgatt ggaaagattc
    42841 tgttgattct atttgctgtg gtgtcgaaaa attttttgtt tgtccttccg tttttggcgc
    42901 ttcctttttt gaagactcta tatggtttgg tcttgtcact ttcgaagaag ttatttcact
    42961 tgtactactt gaagttgttg aagattcggc acttgaaatc gtactacttt cacttgacga
    43021 agttgttgaa gactcggcat ttgaagtcgt actactttca cttgacgaag ttgttgaaga
    43081 ctcggcattt gaagtcgtac tactttcact tgatgaagtt gttgaagact cagcatttga
    43141 agtcgtacta ctttcacttg ataaagttgt caaaagttca ctactctctg ctgttacatc
    43201 ggaattactc atttcagtcg tctcggttct ttcttttgca attttgtatg ttgaattttc
    43261 gcttgccaac acatggaaag gtgttgctat aaagagttgg gacattaata taactgctga
    43321 tgcaagtttt aaagattttt tcatttgatt tacctctctt ttttctcttt aatagtgttt
    43381 tctattaaaa attatgataa a
//
LOCUS       DAJSVF010000018        43318 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSVF010000018 DAJSVF010000000
VERSION     DAJSVF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.018"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            591..1280
                     /locus_tag="ON550_000929"
     CDS             591..1280
                     /locus_tag="ON550_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954030.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ON550_000930"
     CDS             1267..2475
                     /locus_tag="ON550_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3954031.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ON550_000931"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ON550_000931"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR3954032.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRSVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ON550_000932"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ON550_000932"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR3954033.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ON550_000933"
     CDS             complement(6578..7273)
                     /locus_tag="ON550_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR3954034.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ON550_000934"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ON550_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR3954035.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ON550_000935"
     CDS             complement(8472..8663)
                     /locus_tag="ON550_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR3954036.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ON550_000936"
     CDS             complement(8684..9574)
                     /locus_tag="ON550_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR3954037.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ON550_000937"
     CDS             complement(9561..10328)
                     /locus_tag="ON550_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3954038.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ON550_000938"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ON550_000938"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR3954039.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ON550_000939"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ON550_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR3954040.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ON550_000940"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ON550_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR3954041.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ON550_000941"
     CDS             14899..15282
                     /locus_tag="ON550_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954042.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ON550_000942"
     CDS             complement(15450..16208)
                     /locus_tag="ON550_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR3954043.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ON550_000943"
     CDS             complement(16226..17044)
                     /locus_tag="ON550_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3954044.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ON550_000944"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ON550_000944"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR3954045.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ON550_000945"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ON550_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR3954046.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ON550_000946"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ON550_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR3954047.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ON550_000947"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ON550_000947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR3954048.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ON550_000948"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ON550_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR3954049.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ON550_000949"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ON550_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR3954050.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ON550_000950"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ON550_000950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR3954051.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ON550_000951"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ON550_000951"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR3954052.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="ON550_000952"
     CDS             27086..27385
                     /locus_tag="ON550_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR3954053.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ON550_000953"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ON550_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3954054.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="ON550_000954"
     CDS             27973..28209
                     /locus_tag="ON550_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR3954055.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ON550_000955"
     CDS             28352..30325
                     /locus_tag="ON550_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR3954056.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ON550_000956"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ON550_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR3954057.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ON550_000957"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ON550_000957"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR3954058.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ON550_000958"
     CDS             33204..34496
                     /locus_tag="ON550_000958"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR3954059.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="ON550_000959"
     CDS             35232..36095
                     /locus_tag="ON550_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3954060.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="ON550_000960"
     CDS             complement(36224..37846)
                     /locus_tag="ON550_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3954061.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="ON550_000961"
     CDS             38095..38616
                     /locus_tag="ON550_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3954062.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ON550_000962"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ON550_000962"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR3954063.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="ON550_000963"
     CDS             40697..41515
                     /locus_tag="ON550_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR3954064.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="ON550_000964"
     CDS             complement(41618..41815)
                     /locus_tag="ON550_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954065.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="ON550_000965"
     CDS             41984..42562
                     /locus_tag="ON550_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3954066.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42657..42794
                     /locus_tag="ON550_000966"
     CDS             42657..42794
                     /locus_tag="ON550_000966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954067.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT        13193 a         8285 c         8538 g        13302 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gagcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctttg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttat
//
LOCUS       DAJSVF010000019        41865 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSVF010000019 DAJSVF010000000
VERSION     DAJSVF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.019"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..678
                     /locus_tag="ON550_000967"
     CDS             <1..678
                     /locus_tag="ON550_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR3954068.1"
                     /translation="IENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKY
                     DNLDYPAKTSDGSGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGI
                     YSDTIQAKNDKQTLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGF
                     YQLLLSLEQELPAFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLA
                     DGFFEHE"
     gene            788..1222
                     /locus_tag="ON550_000968"
     CDS             788..1222
                     /locus_tag="ON550_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954069.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(1481..1642)
                     /locus_tag="ON550_000969"
     CDS             complement(1481..1642)
                     /locus_tag="ON550_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954070.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(1661..2683)
                     /locus_tag="ON550_000970"
     CDS             complement(1661..2683)
                     /locus_tag="ON550_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR3954071.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            2811..3164
                     /locus_tag="ON550_000971"
     CDS             2811..3164
                     /locus_tag="ON550_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3954072.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            3176..4342
                     /locus_tag="ON550_000972"
     CDS             3176..4342
                     /locus_tag="ON550_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3954073.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(4434..5054)
                     /locus_tag="ON550_000973"
     CDS             complement(4434..5054)
                     /locus_tag="ON550_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR3954074.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5246..5542)
                     /locus_tag="ON550_000974"
     CDS             complement(5246..5542)
                     /locus_tag="ON550_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3954075.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5746..6396
                     /locus_tag="ON550_000975"
     CDS             5746..6396
                     /locus_tag="ON550_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3954076.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            6412..8139
                     /locus_tag="ON550_000976"
     CDS             6412..8139
                     /locus_tag="ON550_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954077.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            8136..10028
                     /locus_tag="ON550_000977"
     CDS             8136..10028
                     /locus_tag="ON550_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954078.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            10732..11601
                     /locus_tag="ON550_000978"
     CDS             10732..11601
                     /locus_tag="ON550_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR3954079.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            11810..12835
                     /locus_tag="ON550_000979"
     CDS             11810..12835
                     /locus_tag="ON550_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3954080.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            12874..13824
                     /gene="manA"
                     /locus_tag="ON550_000980"
     CDS             12874..13824
                     /gene="manA"
                     /locus_tag="ON550_000980"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR3954081.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            13918..15621
                     /locus_tag="ON550_000981"
     CDS             13918..15621
                     /locus_tag="ON550_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3954082.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            15623..17656
                     /locus_tag="ON550_000982"
     CDS             15623..17656
                     /locus_tag="ON550_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3954083.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            17667..18107
                     /locus_tag="ON550_000983"
     CDS             17667..18107
                     /locus_tag="ON550_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3954084.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(18307..19434)
                     /locus_tag="ON550_000984"
     CDS             complement(18307..19434)
                     /locus_tag="ON550_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954085.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(19437..20114)
                     /locus_tag="ON550_000985"
     CDS             complement(19437..20114)
                     /locus_tag="ON550_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954086.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20378..22144
                     /locus_tag="ON550_000986"
     CDS             20378..22144
                     /locus_tag="ON550_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR3954087.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            22159..22596
                     /locus_tag="ON550_000987"
     CDS             22159..22596
                     /locus_tag="ON550_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3954088.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            22609..23760
                     /locus_tag="ON550_000988"
     CDS             22609..23760
                     /locus_tag="ON550_000988"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR3954089.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(23822..24520)
                     /locus_tag="ON550_000989"
     CDS             complement(23822..24520)
                     /locus_tag="ON550_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR3954090.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(24513..25865)
                     /locus_tag="ON550_000990"
     CDS             complement(24513..25865)
                     /locus_tag="ON550_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR3954091.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(26088..27488)
                     /locus_tag="ON550_000991"
     CDS             complement(26088..27488)
                     /locus_tag="ON550_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR3954092.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            27649..28386
                     /locus_tag="ON550_000992"
     CDS             27649..28386
                     /locus_tag="ON550_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR3954093.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            28511..28990
                     /locus_tag="ON550_000993"
     CDS             28511..28990
                     /locus_tag="ON550_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR3954094.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            28987..29754
                     /locus_tag="ON550_000994"
     CDS             28987..29754
                     /locus_tag="ON550_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954095.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            29984..30553
                     /gene="lepB"
                     /locus_tag="ON550_000995"
     CDS             29984..30553
                     /gene="lepB"
                     /locus_tag="ON550_000995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3954096.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(30657..31259)
                     /locus_tag="ON550_000996"
     CDS             complement(30657..31259)
                     /locus_tag="ON550_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3954097.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            31365..31834
                     /locus_tag="ON550_000997"
                     /pseudo
     CDS             31365..31834
                     /locus_tag="ON550_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31940..32524
                     /locus_tag="ON550_000998"
     CDS             31940..32524
                     /locus_tag="ON550_000998"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR3954098.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            32527..33600
                     /gene="prfA"
                     /locus_tag="ON550_000999"
     CDS             32527..33600
                     /gene="prfA"
                     /locus_tag="ON550_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR3954099.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            33593..34432
                     /gene="prmC"
                     /locus_tag="ON550_001000"
     CDS             33593..34432
                     /gene="prmC"
                     /locus_tag="ON550_001000"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3954100.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            34425..34604
                     /locus_tag="ON550_001001"
     CDS             34425..34604
                     /locus_tag="ON550_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954101.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            34626..35651
                     /locus_tag="ON550_001002"
     CDS             34626..35651
                     /locus_tag="ON550_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR3954102.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            35826..37070
                     /locus_tag="ON550_001003"
     CDS             35826..37070
                     /locus_tag="ON550_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR3954103.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            37223..38170
                     /locus_tag="ON550_001004"
     CDS             37223..38170
                     /locus_tag="ON550_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3954104.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            38304..38933
                     /gene="upp"
                     /locus_tag="ON550_001005"
     CDS             38304..38933
                     /gene="upp"
                     /locus_tag="ON550_001005"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR3954105.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(39079..39315)
                     /locus_tag="ON550_001006"
                     /pseudo
     CDS             complement(39079..39315)
                     /locus_tag="ON550_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39436..39508
                     /locus_tag="ON550_001007"
     tRNA            39436..39508
                     /locus_tag="ON550_001007"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39469..39471,aa:Thr,seq:cgt)
     gene            complement(39627..40265)
                     /locus_tag="ON550_001008"
     CDS             complement(39627..40265)
                     /locus_tag="ON550_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3954106.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(40388..41020)
                     /locus_tag="ON550_001009"
     CDS             complement(40388..41020)
                     /locus_tag="ON550_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3954107.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            41371..41670
                     /locus_tag="ON550_001010"
     CDS             41371..41670
                     /locus_tag="ON550_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3954108.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        14047 a         7386 c         8425 g        12007 t
ORIGIN      
        1 atagagaata atccatatat ttctgatgag tcagcttaca aagaagtaat gacacttctc
       61 aaggaagaaa aaacacctca gttcattcat ttagtcacga tgcagacaca tatgccctac
      121 gatggaaagt atgataatct agactatcct gcaaaaactt cagatggaag tgggacactt
      181 gacttagaga attatctgca agatatttcc tatagcagca cggcactgaa gcagttcacg
      241 gaagaattga agaatctttc tagaaggact ttagttgttt tttgggggga tcatcttcca
      301 gggatctatt cagatacgat ccaagcaaaa aatgacaaac aaacattgca tgaaacacaa
      361 tttcttatgt tcgacagtaa aggagaatta gaaaagcaaa cgactcacga tgcaatcacc
      421 agtccgtttt attttgcagc gaatctaatg gaacagacga atcagacaac aaatggcttt
      481 tatcagctgc ttttatcatt agaacaggaa cttccggcgt ttgaacgaga actgtactat
      541 caaaacggac agtggtataa agaagcgcaa ttcaatcgct cgcaacaaga gatttacgac
      601 gaatatcaat tgatccaata tgacattgtc gcagggaaac aatatagttt agcagatggt
      661 ttctttgagc acgaataaga atggcgagct gtatagaaac cattaaattt tttcaagtac
      721 ttgtcatttg aggataaaaa caagtagaat aaataataac aacaatggag aaagtaggaa
      781 aacgaacatg ttaccgaata ttggctatta tgtgcagtcc atcgatcaaa tcgtcaagga
      841 aacagaagca atcggagaag gaatgcatcc gaaatatgaa gtgatcagaa acgcaattga
      901 tgaaggaaaa acaagtgaac tggacaaatc gactcttgaa gagatcatcc tgttatttga
      961 agaaggaaca aaaaaatatc gggcaatgct tatgaaagtc agtactttgc gtccaccagc
     1021 aaaagcaatg ggcattcata aaaattttga aaaagcttat ctttcatatg tagcaggctg
     1081 tgaagaaatg atccaaagta tcgatcttga atcaggggta gatgaagatc tattcaatac
     1141 atctgaagca aaacaagacc aagcgacaga ccaattgaca gctgccatcc aaaaaatgac
     1201 taatttgttg ttgaaaaaat aaaagaaatc tttgataaca ataaaaacta gagttatcta
     1261 atataaatgt aatattacct ttatgataga gtgatcaaaa tcctttatga taatgacata
     1321 cctataacca acaacaacct tgtacttact caaaatcgag taaatactcc ttttccgtcg
     1381 agtctaacca tctcggcggt tttttatttg tagaaaagaa catcaggata gacggaaaag
     1441 tcttgtcctg atgttctttt cagatagtat ggaaagctga ttagttttct acattctgta
     1501 catattgttc aaagtagttc tgtaaatcgg ctttgagttt ctgatattcc tcttcgctat
     1561 actctttatc ttcgattttc ccatcaaaga aaggaatatt tagttcttcc cgtagtctgt
     1621 ctaatacgtc ttgctgattc atttcattct ccttttctta ctaacgatca aaataaacac
     1681 agacacattc tggtacatag acatcttttt tcagtaattc cagtaatcct gtttcaggat
     1741 gacggcgata taatgtcaga ttatcggtgt tctgattggc acacacgaca aaattattgc
     1801 ttggatctag tgcgaaatcg cggggaaaat ccccttctgt tgagatgatt tgcttgtttt
     1861 gaatgtggga accatcttca aaaactgcga atacagcaat cgagttatgt cctcggttag
     1921 aagcataaag gaacttgcca tcatttgaaa tgcggatagc tgcccctcca ttttcaccgt
     1981 cgaattcttc tggcaaggtc gatattttct gtaattcttc aaaagcacct gtttctttat
     2041 tataagaaag cacgcggact gtgctgtcta attcaccaaa taaataagcg aattgtccat
     2101 ttggatgaaa gacaagatgg cgagggcctg ttcctggctc tgcagtgaag gtagttacaa
     2161 gattcaattt gccgttttcg gacacatcat aagtatacac acgatctgta ccaagatcac
     2221 atacgactaa tcgttggtca ggagtcagat ccgtatagtg gacatgcgca tgatcttggt
     2281 ttttatgagg acctgtcgct tcttgatgga atattgaatc tgctgctttc agttcgccat
     2341 ctcctaatat atggtagaca tttacttcac ctttatgata gttcgccccg tatagtaatt
     2401 gacgtgcttc atcaaccgct acataacaca aaggagcgcc agactctgtt accgcattca
     2461 aaaaatgaaa atccttatcg tacgcaccgg caccacctaa tccatccaca ctagtaactg
     2521 tgtaaagatt gccagcttta ctttttgcta cataggtagg gctattttct tcaacagcta
     2581 atgaaaggtt ggctaattct tctttttctg tatcaaggtc gatcgtatag attcctttac
     2641 tttcgcgacg cgtataggtt cctaaaatta tcttttcgat catatgagct gattcctcct
     2701 aattgtaatg aaatagtttt cgtttttatt ctatcacata attattccac acggctaaag
     2761 aatgagaatg tgttatactg tttttgaaag caattaaagg agagagtgca atgattcagg
     2821 caaagattac agaaattggg acagaagcaa taaacaagga agaacccatg ctgattttgt
     2881 tcgataaaac agcaacagct gccttacgaa aatattcggt gattcaagaa gtatcaaaag
     2941 acgaaccttt ttctttgaaa gcaggcggaa caattgcttt tgatgatcag gtatatacga
     3001 tcgattatgt cggtccgatg gccaatggga atttaaagac agtagctcac gtatcattga
     3061 tttttgatgc ttgtccagaa gaaggacaaa ttgcaaatgg aatctatctt catccgcata
     3121 gactgcctgc tattggtttg ggaagccaaa ttttttataa ctaacaggag gaattatgga
     3181 aggaaaaaat aagaagaaca gaccggtatc gtggttttgg cgctggtttt taaataatca
     3241 agtagtcaca gccttacttg ttgtattgtt aatattgctg atcgtctttt tatttacaaa
     3301 agtttcatat ttgtttgcac caatctggca atttttagca atcgtaggtc tgcctatcat
     3361 tttagcagga atcttgtatt acttgatgaa tccggtagtt gattactttg aaaagcgaaa
     3421 agtaccaagg ctttacagta tcattgggct gtttgttttg gttgttgcac taatcgcatg
     3481 ggggagcgta gtgatcatcc ctaagattca ggaacaaaca gtgagcttca tcagtaactt
     3541 ccctcaatat gtagatacga ttgataataa attaaccgag attttgcggg acccgctgtt
     3601 caaccaattt cgcgaacagt tggaaacagc aggtgataag ttcatgaatt cggcaggcga
     3661 tatgatccaa gatatatcta agagtacagt tcagagtctt ggaagttttg tcggcgcggt
     3721 agcgactatt ttagtggctc tgctgacgat gccgtttatc ttattttatc tattgaaaga
     3781 cggtaaacaa ttagcacctt attttgtcaa atttttaccg acacgcatgc aaaaaccgac
     3841 gttgaatgtg ctgagtgaaa tgaatagtca agtttcttct tatataagag gacaactaac
     3901 tgtggctttt gctgtggcaa tcatgtttat gattggtttt gcggtcattg gtttagagta
     3961 tgctgtcacg ttggggatta ttgcgggttt tttaaacctg atcccttact taggatcatt
     4021 tttagcgatg attccggcta tctttttagc cattgtagca ggtccattca tgatcatcaa
     4081 agtactgatc gtttttgcag tagagcagac aatagaagga cgcttgatct ctccattagt
     4141 actagggaat cagttagcaa tccatccagt aacgatttta ttagttctgc tgacttctgg
     4201 gaaattgttc ggaatcatcg gtgtgatctt ggggattcct gtttatgcag cagctaaagt
     4261 agtgattact catgtttttg aatggtacac aacaatctct tctctttatg aagaaaatcc
     4321 gttgaaaaag cagaaagaat aacaaaaaga gacagatggg aaaatatcca aggcacaaat
     4381 gctgctttga agattttttc ctctgtctct tttgtatgca tttatctcaa tttttaaaat
     4441 gatttaccta aagcagcagc tttatctaat gcattttgca tcaactcatc tgcacggtca
     4501 gggtcataat cgattccttc gatgaatagc tgatctactt gatcgatgcc tacaaagttt
     4561 aaaatacctt ttacatattg agaggcaaag tcttgtccgt tgtatacacc gccattagat
     4621 tggatgtgca gtgctttttt acctgtcact aatccttctg gcccgttttc tgtatagcgg
     4681 aacgtttttc cagctacatt gacggtatcg aaccaagctt ttaattttgt tgggatattt
     4741 agattccata aggcgtttgc gataacgatt ttatcagcag caaggaactg atcagtcaat
     4801 tcattgaaac gagcaatttt ttgttgttga ctttctgata aggaagtgaa ttctgcacca
     4861 gcacgtaatg cttcccaacc tgataataat tcttcatcga tttccggaat atcatcgcga
     4921 taaagttcca aaacagtcac ttcatcatct ggatgatttt ctttataact tgttaaaaaa
     4981 gacgtcaatg ccttaactgt gcgcgattct tctgcagtta ggggatgacc tttgactact
     5041 aaaacagatg ccatatgctt accttctcct tttattgcta tgattgttat aactatgact
     5101 aacaaaatca gtttactcga aaaagaaaag gctatcaagt catattgata aggttgaaga
     5161 ataaataact aaaaaaagac agggtataaa agaggctgtg acacgatttt ttgtcacagc
     5221 ctcttttgaa tctaacaagc aatattcaag caacactcga ctgaagagct tccatcccac
     5281 caatgatata aacagcacga aaaccattag cagtaagttg atcggctaat gcttctgatc
     5341 gcctgcctgt atagcttaga atataataaa gtttctttgg atctagattt tttaattgtc
     5401 ttggcaagat agcagcgggg atagaaagcg agtggcttaa gtggtttttt gtgtaatcta
     5461 aggtatttct tgtatctaaa acgatgctgt cagcatcgag aggcaattta ttgaagtctt
     5521 ggacactaat tgattgggcc atttatctca tcctttgttt agtatgtggc tattttaatg
     5581 ggcaactctc attaattcaa atggaaggat tcctcttatc acttttttaa tgtatcggat
     5641 gataatgaaa aaaactatgc aaatgtcttg caaaacaggt aggaggtgtt ataatgacat
     5701 agcgtcatat gacaatgtgt catcttgaaa gaaaggagaa tccatatgcc gacacagaca
     5761 tttttccatt tgcctaaaga aaaacaaaaa agactaatcg aggctgcgag aatcgagttt
     5821 tctagagtac ccttaaaaga agcttcgatt gctaatatcg tcaaattagc ggatatccct
     5881 agaggcagtt tctatcagta ttttgaagat aaagaggatc tttattttta ttactttgag
     5941 acaatgagac gtgacagctc taaagatatg ttacaactaa tgaaagaatc cgatggtgat
     6001 ctattcaaag gattcgaatc ctatttttct aaaatgattt atgagatttt ggaaggcgag
     6061 aatgcttctt tttatcgtaa cttgtttatg aacatggatt accgtgcctc aagaaaagtc
     6121 gctcctcata cgagtcgcca aaaaacgcct cacgatccac ataaagaaca caaacaagat
     6181 gcaaaagagt tgcttgaatt gatcgatcgg acgaagctga aagtaaaaaa tgatcatgaa
     6241 ttggaattac tgatccaatt agtgatgaat actgtattta caacaattgc gcattcgtat
     6301 cgcttatttt caacatctga agattactca gtagagcaag caatcagtga attcaatttg
     6361 aaactttctt ggctgaaaaa cggagtatat aaatagaaag aagggaacta gatgctaaaa
     6421 atcgtaaaac ggatctcctt gacttcagca attgcagctg cagttttcat ggtgatccaa
     6481 gtactggcag atttatattt gccgacactt acctccaata ttattgataa aggggtagcg
     6541 caaggagatg tcgattatat ttggcataca ggttttgtga tgatcggctt ttctttgatc
     6601 agtatcttag ctgctatcgc aaatacattt tttgctactc gtgaatcaca gaaactgggt
     6661 aagcaattgc gtacggatgt ctataaaaaa tcagagagtc tgacaaaaga tgcgtttgat
     6721 aaatatggaa cggcctcttt gatcacaaga acgaccaatg atgtgactca aatccaaatg
     6781 gttacacaaa tgtttttacg tatgatgatc aatgcaccaa tcacgttgat tggcgcgagc
     6841 atacttgctt atcaaaaaga tcatcagttg acaaaaatct tcttagttgt catcccagta
     6901 atgattattt tgatcggcgg aatcatgtac tttgctgttc cattattcaa aagtatgcaa
     6961 aagaaaactg accgtttaaa cctggtattt cgagaaggat tgacaggggt cagagtgatt
     7021 cgagcattcg ataagacacg ctttgaagaa aatcgttttg atttagcgaa taaagattat
     7081 acaaatacgg caatcaaagt taatacgatc gtcgcattga tgatgccaat gatgacattg
     7141 atcatgagcg gtacaaacgt agcaatcact tggtttggtg gacactacat agcagatatg
     7201 actttggaag tcggtaatct gattgcattt atgacttatg cgatgcagat cttgatcagc
     7261 tttatgatgc tttccgctat ttttatcatg gtgcctcgtg cacaagcttc agctgaccgt
     7321 attaatgaag tgttggatga aaaaatcggt atccatgacc ctgaaaatcc taaaacagtt
     7381 tcttttgctg gaaaaaacgc aacattggcc ttcaatcatg tcaattatcg atatcatgga
     7441 gcagaaaaac ttgcattgga agatattgat ttccaagcca agtcgggcga gatcgtcgca
     7501 atcatagggg ggactggttc aggtaaaacg accttagtca atttgatccc tcgattatat
     7561 gacatcgaat ctggttcgat ccaaataaat ggaacggaca ttagtgacat gactcaatat
     7621 aatttgcgag aattaatggg ctttgttcct caaaaagcag ttctgttctc tggaacgatc
     7681 cgagacaata tgcagtatgg taagccggat gcaacagatg aaatgatttg gaaagcatta
     7741 gaaatcgcac aagctaaaga ttttgtttca gagatggaag atggcttaga tagccatgtg
     7801 gaacaaggag gaggaaactt ctctggtgga caaagacagc gtctggcaat tgcacgggca
     7861 ttagtcaaaa cagcagatat ctatgtgttt gatgattcct tctcagcgtt ggatttcaaa
     7921 acggatgcca atcttcgcca agcattgaaa acgaatatga aagaaagtat caccgtttta
     7981 gttgctcaac gagtaagtac agtaatggat gcagatatga tccttgtgct agatgaagga
     8041 aaactggttg gcaaaggaac acatgaagaa ctattagcaa caaacgaaac ttatcaagaa
     8101 atcgttcatt cacaactaag agaggaggac ttagcatgag taaaacaaac acacgtccct
     8161 ctcatggacc agcaggcggt ccaggacatg gtggacctgg cagaaatctc ggtgtaaaag
     8221 gtgaaaagcc taaaaatttc tggggaacgg tacgccgatt gtttggctat atgtctaaac
     8281 gtatggttgc gatcatcgct gtatttgtac tagcaatagc agccgtgatc ttccaaatcc
     8341 aaacaccaaa ggtcttgggg aaagcaacca cagaaatatt caaaggtgta atggctggcg
     8401 ctcaacagat gcaacaaggc caacaagtaa caaaatttcc gatagacttt gataaaatcg
     8461 cgcagatcat tgcgacagta atcgtgatgt atttgatttc tgctgtattc aactttttac
     8521 agcaatttat tatgacacgt gtttcccaac gaacagttta tgaattacgt catgatttgg
     8581 aagccaaaat gaatcgtgta ccgatttcct attatgatac acatacaaat ggggatatca
     8641 tgtctcgagc aatcaatgat atggataaca tcgcaagcac gctgcagcaa aatttgactc
     8701 aatttgtaac tagtgcagtc acattgattg gggtcatcgt gatgatgctt tcaatcagct
     8761 ggcaattgac gttagtcgcg ttattgatga tccctttaag cttgatcatc gtgatgatcg
     8821 tagcgccgaa atcacaagtg tttttcgcag atcagcaaaa aagtttaggt ttattgaata
     8881 accaagtaga agaaacatat ggtggtcata ccattgttaa aacattcaat catactgaaa
     8941 aagaccagga agtatttgaa aaagaaaatc agaatctgta tgccgctggt tggaaagctc
     9001 aatttatttc ggcaatcatc atgccgttga tgaatttcgt taaaaatcta ggctatgtgt
     9061 tcgtcgctgt actaggcgga gtaaaagttg caaatgggaa catgacatta ggggatgttc
     9121 aagcgttctt gcaatataca acacagttct ctcaaccaat cactcaactt gcaagtttga
     9181 tgaacactat ccaatcaaca gttgcttcag ccgaacgggt atttgaagtg ttggatgaag
     9241 aagagatgtc caatgaaaaa tctggccttc cagaagaaaa agatacacca tacaaagttc
     9301 gttttgaaaa tgttcaattc ggctacacac cagataactt gctaatgact gattttaact
     9361 tggatgtcaa acctggtgag atggtcgcta ttgtaggtcc gactggtgca gggaagacga
     9421 cactgatcaa ccttcttgaa cggttctacg atgtttctgg aggtagtatt cgttacgatg
     9481 gtgtagatac ccgtgatctt tcacgtgatg aattgcgcgc acagttttca atggtactgc
     9541 aagatacttg gctgtttaca ggaagtatct atgacaatat caaatatggg aacgacgatg
     9601 caacagacga acaaatcatt gaggctgcta aagctgctca cgtagacgat tttgttcgta
     9661 aattgccaga aggatataac actgtattaa acgaggatgc aagcaatatt tcgcaagggc
     9721 agcgtcagct aatcacaatt gcgcgggcat tcttagcaaa tccagatgtc ctgatccttg
     9781 atgaagcaac atctagtgta gatacacgaa cagaaatact gattcaaaaa gcgatgaacc
     9841 gcctattgga aaaccggaca agtttcgttg ttgctcaccg cttgtctaca atacgcgatg
     9901 cagacaacat tattgtgatg aatcatggtt cgatcgtaga aacaggaaac cacgatacat
     9961 tgatggaaaa agacggattc tatgcagatc tttataacag tcagttttct gaagaagaag
    10021 cttcatagaa tagtacaaat ttattatttt ttttaggggc tgtgacaaaa gtcgtgtcac
    10081 agtccctttt tccgaataaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa
    10141 cggcaaaata tgagaagcta tttttccgtt ttgtatctct aatactcagg tcaaaccgct
    10201 gattttacaa cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    10261 atagtcaaca aacctgagaa gctgtttttt gaaattgctc cttgtgactc ggagttaaac
    10321 ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc agtttctcgg tattaagcca
    10381 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa
    10441 gcgacttttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    10501 gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    10561 cagaacagct ttttcacaac ctctttttat aagagttttt actcattaat ttacaaaaaa
    10621 caagcaaaat gcgaattttc tcacattttt tttaacaaaa tcaaagttat tgcttgaaag
    10681 aagaagaatc catcgttaaa ataaagcttg acgtagaggg gagcaagaaa tatgaaagat
    10741 tttcaattgg gggctgccac ctatttaaaa agtgtagcca ttcgggtgaa agaccgagat
    10801 aaaatgattg atttttacaa gaacgttgtt ggctttgatt tgaaaagaga agaaaacgaa
    10861 ttagccattt taggaacaga agaagctgga agtgaaatgt tgctgctgga agaaagtcct
    10921 agagcgaatg atcacatggg ggtgatcaaa aagctgaacc gtttttcatt gattatccct
    10981 agcacagaag aaatggcaga tatcctttgt cgtatccgta aaatgaatta tccaatcaaa
    11041 gaagctttag aagatcatgg acgtatgggg attttgctcg tagacccaga agagaatgaa
    11101 ttggaaattt accatgaaga gaataagaac gaccaaagag aaattccaga aacaatggat
    11161 caagaagcat tattaagaaa atctgaagaa aaattcagta aactatcaac aggttcttat
    11221 tttgaaaaaa tccatttaaa tgttactaac ttaacaaaag aacgccaatt tcttaatcat
    11281 gtacttggtt tgaaggtaca taatgaagaa gagggcatcc atgtattgaa tgaaggagat
    11341 ttccatgttg gtttgacaga agctagcgga ggcgcaattg atttgcctac agatgaagtg
    11401 attggacttg attttctgaa attcatgatc gatactgaaa gcatggaagg actgattgct
    11461 cacttgcagc aactatcagt tgatttcttc ttagacaaaa agcacacagt actcacaatc
    11521 tatgatgcta taggaatcga atggtggttc gtcagtgagc caaaagaaaa gaataaaact
    11581 gacattcgat gcgacgctta actttttctt tcttaacgat tgtcgcatgt ctcgtcattt
    11641 tatgaacggt catactatcg cttattctag agatagaaag agaaaatagt ataccggggg
    11701 aaactgcgcg gtttttcgcg cagttttttt gtgtaaaaaa ggttgattcc tcatagatta
    11761 agctttaagc ttggactgta agaatctgat agaaaagcag gagaaaaaga tgtatccaca
    11821 cacgaaacag cttattttga aaaagatgga agaaggattt tatccaggtg tagcagtcgc
    11881 cttcataaaa gaaaatcaag tagaaacatt tacagccggc tttgcccaag tcgttcctga
    11941 aaagcagctg atgacagaag atctgttgtt tgatgcagcc tcactgacga aagtcgttgc
    12001 aaccacttca ttagttttac gtctattaga agatggaaag atagaactcg atcaaccgct
    12061 tcataaatat cttcctgaat ttgaaaatca gacaatcact ttgcgtcatt tactgacaca
    12121 tacttctgat atcaaaacat ggataccaaa cagagataag ctatctcagg aagaattgat
    12181 cgccgcttat ttgaagctag aaccaggtga gcaactagga aaagaagtaa aatatacaga
    12241 tgccggtacg attcttcttg ggttgatgct agaggaaatt taccaagaaa ctgcagtgga
    12301 aatctttcaa gaacaagtat tggaaccatt aggaatgatg aacagcttat ttcttccttt
    12361 tccttccaaa aaaatcgttc ccacagaaca actgccttcg ggtgaggttt tgcgtggaca
    12421 aactcatgat ccgaaagcaa gagttttggc tgaacatgca ggtaacgcgg gtctctttat
    12481 tgatttgaaa gacagcatta cttttgtcca aatgtatttg aacaagggaa aaatccgtaa
    12541 tggtcgtttt ttagaagaag agacgattga ttctttactg caagatcaaa caccgaaaaa
    12601 aaatggctat cgttctttag gatgggactt aaaaaaggat gtagaagacg gtcatgcgat
    12661 tctttttcat actggttata caggtacgtt tttcttgatt gatataaaaa cacaagaagc
    12721 gttgatattt ttgtcaaatc gcgtacatcc tgtcgatcat cgtgcggagt atctcatatg
    12781 tcgagatgaa attgttgaaa actttttgaa agagaaagcg gtattaccga aataatgcta
    12841 taatagctat gaaaaatgag aggagtttga attatgcagg aacctatgtt tttaaaacca
    12901 gtttttcaag aaaaaatttg gggaggaagt cgtttacgtt cagtctttgg ttttgatatc
    12961 cctaacgata aaatcggaga agattgggcg atcagtgcgc atcctcatgg tgtcagtgta
    13021 gtagaaaatg gcgaattcaa agggaaaaga ttagatgaac tatggtcaga acacaaggaa
    13081 ttgtttggcc atccttcaga accagtgttt ccattattga tcaagatctt agacgcagaa
    13141 gatgatctat ctgtacaagt acatccagat gatgcctatg ggatgaaaca tgaaggtgaa
    13201 ttaggaaaaa ctgaatgctg gtatatcatc gatgctgaac ctggtgcaga aatcatctat
    13261 ggtcatcatg caaagacaag agaagaacta gctgagatga tcaaagatgg gcgctgggat
    13321 gaccttttga agaaagtacc agttaaaaaa ggtgatttct tttatgtacc aagcggaacg
    13381 atccatgcta ttggtaaagg aatcatgatt ttagaaacac agcaaagttc tgacacgact
    13441 taccgcgtgt atgactacga cagaaaagat gcgcaaggac aaacaagaga gctgcatatc
    13501 caacaatcga tcgatgtaac aactgtccct gcgaaaacac cagaattaca aatcaaagaa
    13561 gtccgaaaag ggaattcatc gatcgtcact tatttagaaa ctgagttttt caatgtatat
    13621 gagtgggata ttaaaggaat cacatctttc aaaaaacaag ccccttatac attgatgacg
    13681 gtaatcgaag gagcaggaga ccttgtcgtt gacggaaaaa catatccatt agaaaaagga
    13741 acaagctgta ttattccaaa tggcgtttct gagtgggcgg tccaaggtga actagcaatc
    13801 attgcgtctg aaccaggcaa atagttccca gatagctgaa agcagcagac ggtttttcgt
    13861 ttgctgcttt ttctacataa aatccgtcca tcaataaata gaagtctggt gaaaaagatg
    13921 gaatacaaat ggaaagcaaa caacagcagt cgcgtgtcta tgaaacaact gagaaaagct
    13981 ggacatcact taaatgaaat gatcatgcca aatctaagtc tgtttatcgc ttggggaatc
    14041 ttgtcattag caacaaaagg tgtgtctggc gatttgcaca ccacattaga agaggtatgc
    14101 cattggatga tccagctgct tttacccatc ttgatcagtt attcaggcgg gaaatcggta
    14161 gggggacaaa aaggggcagt cgccggtgca attgcttctt tagggttgat cgctcgttcc
    14221 aatgctccac aaatcgtagg agcgatgatt atcgggccgc ttgccggcat cgtatatgaa
    14281 caatttgttt ctagattcca tgtgaaattc aaggctggat atgaaatgct tgtcagcaac
    14341 ctactagtag gactgactgg tagcgttatt tgtataattg gaatcgtttt tattgaacca
    14401 gttttaaata gtgttcactt gttgctcgcc tccgttgtgt cttggctagt cacaatgaat
    14461 ctgttaccac ttgtcagtct gatcttagaa cctctaaaag tactattttt caataatgca
    14521 atcaatcatg gtgtattgac acctttagga atcgaattct cacaaacagt cgggtactct
    14581 tccttatttt tgatggaggc aaatcctgga cctggtttag ggatcttact ttctatcctt
    14641 tgttttgcag aaaaacagga aaaagtaaat gcgagtggag cattgatgat ccatgcgatt
    14701 ggcgggattc acgaaatcta ttttccattt gtcttgctgc gaccgtatct atttttagct
    14761 gtaatggctg gtggggcaag cggcacgttg atatttcaat ggttagatac gggattagct
    14821 gcacctgtat cgcctgggtc actactcgtg atttttgcca atgcgcctta tccacaacta
    14881 gctggggtaa gtatagggat agcaatcagt acatttgtga ccttcatttg tgcagcgata
    14941 ctattgaaat taccaaataa agaaaaagag aacaaaacgg tcaaagaaag gggagaaatc
    15001 ttggaaccat ctatcattca ttccgtcatt tttgcttgcg actcaggtat gggatcaagt
    15061 gctatgggag ccgccctgct gaaaaagaaa ttagcagcag aaaaaatcga tctttctgtc
    15121 ggatacagct ctatctatga attgaataac caagtaaatc agctggttat cgtgcaacat
    15181 gaactagcgg gtcttgcaag agaaaaagta cctcaaagtc aaatctatgt actggatcac
    15241 tttttggaaa cagatatctt gctgtcacga ataaaagaag cgatccaatt tccaaagcgg
    15301 gaagaaaaaa aagagatagc agatatcaca gaacaaaaaa cgtcgcctgc taatattgta
    15361 tttttgtata aaaacaacag acgcggctca caaactatgg gcatgacgat tttaagtcaa
    15421 ttagcaaaaa aagcgaaaaa acagtttgtt atctcaaaag aatcaataga aaaaatcaaa
    15481 atccaagaag aaacgattta tatcgcacaa agagaactag ctgtaactga acagctagaa
    15541 gaaaagatcc ctcggctagt tctagttgaa cattgggtag ctacgaatga atacgaaaaa
    15601 ctcgtgaagg gagaaatata agatgtattt atcgcccaga gaaaaacaat tattagcaga
    15661 atttctcagt agcccgtcac ctgtatctat ccaaaaaatg atgaatttat tgaaggtcag
    15721 taaacggact atttaccgcg aattagataa tcttgaagtc tcattgaaat cggtaaatgc
    15781 ttctttgaaa aaagcagcta gaggctcatt tgtcattgag gcatcagaag aagtattaga
    15841 gcaattaaaa gaaaatacag atgggaaaag taatgactta tccactgcta aacgacaaca
    15901 cgcgatttta gccgagttgt tactgacgac agagccgttg agtcttgctc atttcatgga
    15961 atcatatcgc atcagtaata ccacttttta tgcagatatc aaacagttgg aagaaagtat
    16021 tcgccaactg cctttggaaa tcattcggaa ccagggatac gagatcgaag gaccggaaaa
    16081 ataccgccgg cttctgacag cgaatgtatt agaattagaa atcaatgaat atgaactttt
    16141 tcatagtatt tctttcgatg catcactgaa ttattttttt cgattcgttg atccgcaaca
    16201 tctatctctt gcacgcaaag taatcgggga agaattgatc caaaaaaaga ccaatctaag
    16261 tgatagaaag cttgaacatt tggttttgat gctgacattg acgatggagc gtgtgacgaa
    16321 aaatcattta ctgacaaatg agacatataa tagtctggca aacaaagaat tactacagac
    16381 agctaaacga ttgttttcac ggatcgcttc tgagacaaaa caactttatc cagtaaatga
    16441 gatcgtcttt tttgcaggat tattaaatga cttcacgaac tcatttgatc agaatttctt
    16501 tgaagagacg ttcgatacac agcttgctta tttagtaaaa caactgattc aagaagtaag
    16561 tgaagagacg aatgtccatt tttatgaaga tgaaactttg tacaaaatgc tgttgactca
    16621 tttatctggt gtgttttcaa gagccgtttt acaagaagag acactgacca atccgatttt
    16681 agaaaaaatc atggttcaat acgaggaagt tgcgaatgca atccgtaaag cagtagagcg
    16741 gatttttcaa gaaaaaaaat tatccgaaga agagatcgct tatatggtcc ttcattttgc
    16801 gaattcattg gaacgaagtc cgaaagaaag caatatcagt attgcaggat tttcgccaag
    16861 tggattagct tctactagca tgttggaact acgattgaaa aggtattttc ccttcatcca
    16921 acaaattcat ttctatcgga ttgctgattt aaaaaaaatc gatttacaag aagaatatga
    16981 tgttgttatt tctacatcta ttttaccagg atacggtgga aaatatattt tagtctcccc
    17041 gctactgcta gaagatgaag tcaagcagct aaaagaagct ttttcgcata ttgagaagaa
    17101 aaaaaggcaa aaaattacgt ataacagcaa taacgagtgg atgatagaag atacttatga
    17161 ggaaacaatg catttcatgg atcaaatcaa tcaattactc ggtcgttttt ttatccaaga
    17221 actggataat cctgagacga tttccgaaac gattcgcagc ttatttcctt attttcctga
    17281 aggactagta acggatgaag aggtggttca tcgccggctg cttaagcgat atgaacaagc
    17341 acctatcggt attcctcata caaatatggg gttattccat acatccagct cattgatttc
    17401 taaaccgatc ttttgtattt tcaatttgaa acatccgttg accattgaag ggatggatag
    17461 acagccagtc gatttgtccc gtatgttagt tatgctggcg ccttcgccaa tccaagaaaa
    17521 agacgggatt cttttaggaa agatcagtgg agcaatcatc atgaatgact tgaacaccga
    17581 gatcttccgc tcaggaaata aagaaatcgt ttatcagttg ttagcgaaaa tcctgattga
    17641 agaaataaag aaatgaaggt gaaaagatga tacagctacc agcagaaaaa atattgctcg
    17701 atcaatccta tcaaaattgg aaagaagcgt tgaccgagat cggccagtgg atgacccaac
    17761 aagggggaat caaaccggca tatatccaat caatgatcga aagacaagaa aaagcgagtg
    17821 tctatatcgg gaatttcgta gccttgccac atgggactga caaagatgaa gaagtagtag
    17881 aagaaggaat ctatttagtc caagtgcctg atggtgtgga ctttggaaca aaagaggaac
    17941 caaaaatcgc gacactctta tttgtagtgg ctttaaaaaa agaacatcag ttagcggtgc
    18001 tccaagattt agcctttttc tgttcagaca tcgaccaagt aatggcgctc agtgatgcgg
    18061 aaaccattga agaagtccaa atgatttttg aacatggaca tatataacca atgttcacag
    18121 taaaaaaagc tgccttgcat caagtaataa cttcaaccaa gataaaaata agaggagcta
    18181 tttttgttgg gaggttctta ttactgcgga tgcaagcttt tgaaaactat ttattctaga
    18241 aaaccgttta tttgaaatag gggacggtga caaggttttt gtcaccgccc ctattatttt
    18301 tgtgttttaa tagctctttt ttatatagat agtgcccata cctatgaata ataaaacgat
    18361 actccatata ccaagtgtca agataccatt catgaaagta acctgttctg aagctagtgg
    18421 cgagaaaata tctcctccta tgatcacatt ttgaaaatca acataagaag ttggcaagta
    18481 atgactgatt gattctatcc atccaatcga agaaaacaag cgatactctg ctaagaaaag
    18541 acaaagaagt gtacctaaaa gattgactag agaatgttca aataaagcag atagaagaaa
    18601 actgattgta cttaaaaaga gcacccacaa caacaggaac agaaagctct ggaaaagata
    18661 ctcatacatc ggcaggattc gtatctcctg accaatgatc gttcctacag gataacggaa
    18721 gtctcccaaa ccatttttga ttattactag tatagagacg atcaaaaatg gcatcacgag
    18781 acttagcata gctgcaccat aataaatggt aaaacggatg ctatgtttct ttaccatact
    18841 atccggtaat agattgacta gagagattac ctgctttctt ttttcataag agatgatgga
    18901 agaaagaaac aaggcggtta tacataaaat caaaaaacta gaaatcattc ctgaaaaaat
    18961 attttcgtag taatttgcta aagagagatg cgctggcaag tcaaaaatag aatatctttg
    19021 gatctgatgt tctttcatat aacggagcaa ctcgacacgt ttttgttgtt caatgattgg
    19081 aattccttgt ctttgaccag aaatcagtcg atccagtata tctttttcat aatgataagt
    19141 tgcttcgacc atttgctgga cattccctgt gttttttgca tgtaagattg cttcgatcag
    19201 tttgttgctt ttctctaaac tttccacaaa ttctttctca ttctcatctt ctttcaacgt
    19261 ttcaatcaaa cgttgcatct cttcatagtt atgattcagt tcatcgaatt ctgtataagc
    19321 aagtttatga tcaaaaaaaa gtgggaatgc aaagagcacg accaacaata gaggaaataa
    19381 aaagtttact ttgtttttta gtaagagatt gcgttcaaat gtaagtaaag acatagttac
    19441 acctcatata atttacgata aatagctaaa atctcttcca tatttgagga ttctgtgacg
    19501 atttctccat tttttagaaa acatgcttta gaacaaatcg attggatatt cgcgatagcg
    19561 tgggaagaaa gaagaatcgt cttcccctct ttctgccatt gtctgatgag gctattcaag
    19621 atatcaatac ttcctgggtc taatccgttc aacggttcat cgaataacat gacagatgaa
    19681 tcactgatca agtaagcagc aagcaagaca tgttgtttca ttcctagaga catttttcca
    19741 atcggcaaag taaggtactc agtcatcttc aatcgttgga aaacagtttg gcattctact
    19801 ttgctctgcc aatagtattt gatcaaagtc aaataatcaa cagccgatag atagtcggat
    19861 aactgattcg ttgattctag aaaaaagaaa gaccggcgat aatctgtata agcagtagga
    19921 gagatgttgt gaagggacaa ttgatcataa gttgttggca gcagttgggc aatgccattc
    19981 agcaaagttg ttttgccaaa tccgttcggg gcaatcaaag cagctacttc tcctgattct
    20041 actaaaagat tcaattgtcg aatcacacgt tttttaccac gtttcatgtt catgtttttg
    20101 atcagtaatg tcatagatag gcctccaata taaaatttaa taacataata gaatggaaaa
    20161 catctaaatt aaatgctaac aaaagttatt gtttgcatat tataaaaaaa ttaaaataac
    20221 agttttattt aaaattgaag ttttctgata ccgaaatctt tttattttgc tgacatactc
    20281 aaacctttgg cacaaagttt cgtgccaaag tacgactatt tctcttttta gtaagcggat
    20341 acaattagag tatcaaaagg agagagggag agacaaaatg gaacggacga ttcccaaaaa
    20401 caacatttcg ctaaaagcga gggtacaaaa attaggcagt actttatcaa gcatggtcat
    20461 gccaaatatt ggagcactga ttgcctgggg tgtattaacg gctctattta tcccagatgg
    20521 ttatttacca aatgaggctt tcgctaccat ggtaagccca atgctaactt atctgattcc
    20581 tttattgatc ggttatactg gcggaaaagt aattgctggt gaccgcggat cagttgttgg
    20641 ggcaattgca acgatgggtg tgattgtcgg aacagatatt ccaatgatgc taggcgcgat
    20701 gatcatggga cctcttggtg gatatgccat caagaaattt gatcagctat tccaaaaacg
    20761 gatcaaatcc ggttttgaaa tgctagtcaa taatttttca gcaggtctta tcggttttgg
    20821 attagcgtta ttaggttttt ctgcgattgg tccagttgtc gatgcattga ctcaagcaat
    20881 ggcaaaaggt gtagagatca ttttatctgc ccatttgatt ccattgacaa gtatttttat
    20941 tgaaccagca aaaatcttat ttttgaataa tgcaatcaat catgggattt tgacaccatt
    21001 gggaactgaa caagtagcag aggctggaaa atctattcta ttcttgcttg aagcaaatcc
    21061 tggtccagga cttggcgtat tacttgcttt tactttgttt ggcaaaggag cagcaaaatc
    21121 ttcagcacca ggagccatga tcattcactt tttaggcggg atccatgaaa tctattttcc
    21181 ttacgttatg atgaaaccgt tattattctt atcagttata ggcggaggaa tgtcaggaag
    21241 tttcattttc caattgatgg atgctggact aagagcgcca gcttctccag ggtcgatcat
    21301 tgcgattcta gcgatgacac cgttcaattc ttaccttcct gttattttgg gcgtgattgt
    21361 tgccgcagct gtttcgtttg gtatttctgc agtaattttg aaagcggatg cgaaagatac
    21421 aaatgaagac ttcgaaaaat ctgttcaaga gacaaaggca gctaagcaag cttccaaagg
    21481 tgcccaaggt attcctcaag gagctacatt ctctggcgta caaaaaatca tttttgcttg
    21541 tgatgcagga atgggatcaa gtgcgatggg cgcttcgatc ttgagaaaaa aagtacagga
    21601 agcagacttg cctcaaacgg tgaccaatca agcaatcagc caactgacag atgactcgaa
    21661 tacattgatc gttactcagg tcgaactaca agaaagagca aaacaaaaag caccgagcgc
    21721 gcgttttgtc tcagtagaaa actttttgaa ttcacctcga tatgatgaaa tcattcaaga
    21781 attaagcagc aagcagaaaa catcagtttc tgaaattgag aatgagccag cagaagagtt
    21841 tctttcagca aacatcaaac aaatcaaaga gattctactt gtccacgatg atcgttccgg
    21901 atcagcaaca atggggatga ccgtgctcaa agatatatta gacaagaacc atttaactat
    21961 cccgttaaga aaagtcaata tccaagattt aaaagatgaa cctgattcac tgatcgtagc
    22021 caaagaaagt ttagcggaaa aagtacagat ggctgcacct caatccattc atttatcagt
    22081 caacagcatg gtcaatccgc aaaaatacga atcgattgca caatatttga aacagacagc
    22141 ttaagggagg aaaacagcat gcaaagtttg ccattcgaca tgatcgagct caatcgatct
    22201 tttaaaacca aagaagaagc gatccgctat tgcggtcaga tgcttgtaga agcagggtgc
    22261 gttgatgagg aatatatcga tgccatggta gatagagaca aaatgttatc tgtttatatg
    22321 gggaatttta tcgcgattcc tcatggaaca gatgaagcaa aaaaatatgt gaaaaaatcc
    22381 ggcatctgtg tcgttcaagt accagatggt gtagattttg gtaccgaaga agaagaaaag
    22441 attgcgactg ttttgttcgg tattgcagga gtcggagatg agcatttgca acttgtacag
    22501 caaatcgcac tttattgcag tgatatggac aatgttgtcc agctagcaga cgcgctcagc
    22561 aaagaagaag taacggaaaa tctagcaatt gcttaaggag agagaaaaat gagagcagta
    22621 cattttggtg caggaaatat cggtcgagga tttattggag aggttttggc agctaatgga
    22681 tttgagatta ctttcgtaga tgtgaatcac acgatcattg atgctttaaa tgaaagaaaa
    22741 gaatacgaaa tcgaattagc agatgaatca aaagaaaaaa tcactgtaaa aaatgtcaaa
    22801 ggaatcaaca atcaaacaaa tccagaaaaa gtaatccaag aaatcgctga agcagattta
    22861 gttactaccg ccattggccc gaatatctta ccttttattg ctgagttgat tgcaaaaggt
    22921 atccaagaaa gagaaaagga aggaagcaca acatctttag atatcattgc ctgtgaaaat
    22981 atgattggcg gttcagcatt tctagaaaaa gaagtgtata aatatcttcc ggaaacatca
    23041 tttactgaca aatatatcgg ctttccaaat gcagcagtcg atcgaatcgt cccattacaa
    23101 caccatgaag atcctttatt cgtccaagtg gaacctttca aagaatgggt cattgacgat
    23161 agccaaagaa aaaataaaaa aatccaatta aaaggggttt tatatgtaga tgatctagaa
    23221 ccatatatcg aacgcaaact atttagtgtc aatactggtc atgcgactgt cgcttataca
    23281 ggtgccttgt taggttacca gaccattgat gaagcaatgc aagatgcgtt agttgtggca
    23341 caattaaaat cagttttgca ggaaacagga agtttgctga ttgccaaatg ggggtttgat
    23401 gcagagcagc atcatgcgta tattgaaaaa atcattcatc gcttccaaaa caaaaatatc
    23461 tctgatgcga tcacacgtgt cgctcggaca ccattacgta agttaggtta tcaagaaaga
    23521 tttacacgtc cagtgagaga actacaagaa cataacttga cttgcccaca tttgacggca
    23581 acaatgggga ttatttttaa ttactatgat cctgaagatg aacaaagccg tcaattgcat
    23641 gaaatgaaga tccatgaaaa cttggaacaa ttgattcaag aagtcacagg aatcaatgat
    23701 ccgaaaacaa tcgggaatat caagcaaaac gtgaaccgtt atgccaagca agtcgcttaa
    23761 aataggatac gaaaaagggg aatgtgacga aatttttgtc acattcccct ttttcgaata
    23821 actaggctgt ttgtccttca agatagggca gatccaattc tttaccgtaa cgttgttcta
    23881 aagctaggcg gatcaaacga gctgccaggt tctcatttct tgcaaggata ggtccatgga
    23941 aataagaacc gaagacattt cggtaaatga caccttcaga ttggtcttca ccattatttc
    24001 cttgaccttg taccactttc cccaaaggtc gttcaccttc acctaagaaa gtccgaccat
    24061 tgtggttttc aaatccataa taagtttcat tgaattcttc gttatggata acgatatcac
    24121 cgataaaccg gttattttcc tgacttaacg tgtagtgatc cagtgcactg attccttgga
    24181 tcttttctcc attagctccc atgtagtagt gacctagcaa ttggtagcca ccacaaatgg
    24241 ctaacatgac gccatcattt tcgatatagt tgatcaaaga atctttcttt ttctggatgt
    24301 cttttgaaat gattaattgc tcaaagtctt gaccaccgcc aaagaaaacc aagtcatatt
    24361 tatcagcatc aaaaggttca tgtatactaa cgatctccgt atggcaagaa acccccattt
    24421 tttctgctat atatttcaac atcaaaaggt ttccgttatc accatatgtg ttcatcaaat
    24481 ttccataaag gtgggcgata ttgagttcat aattagccac ggttcattcc tcctttgata
    24541 aatccttgag cagctagttc tttacgaagc tgcaatacag cagtgtaagt agctaaaata
    24601 tagacatgct cagttggcaa ttctttgatt ttttcgatta cttctggtaa ttcttttgtt
    24661 tctgtcaatt tattttctgg aatcccggca acttttagac gtaaagtcat atctgtatga
    24721 cggtctcctc cagaaataac agctgggata tccatatcgg caaagttttc aaaattacca
    24781 tcccaaatcc agctgacatc gattccgtct gcgtaattgg catttaataa agccactaaa
    24841 gaaaatggat aaggagctag accaatcata tcgattactt gattgatacc tacaggattt
    24901 ttgacaagta ctaatgtaca ttttttccca ttgatattga tgacttcttg tcggccgaat
    24961 actttctcgt catatcctaa accttgtttg attttatcag gatcgacatt gaagtactct
    25021 gccactgcag tagctgctaa agcattgtag acattataca taccgccaac ttcgattccg
    25081 tattcatgac catcgatcac gaaatctgct gacacattgt ccatccgtac catatcagtt
    25141 aatcgataat ccaattcagg acgtttgaag tcacaatttg gacagtaata tttccctaag
    25201 tttgcataag tgatcatttt ataatgcaag atgtgatgac aatttggaca taagacaccg
    25261 tctgtattgt aatgagccat ttgttcacga tcttcttcat ggtcaaagcc ataatatttt
    25321 ctaggattga ctgtttcgat agaattgaag attggtgaat caccgttact gataatcgtt
    25381 gcatttggtg actttgcggc accgtctaca atcattttat aggtcgtata gatttctcca
    25441 taccgatcca tttgatcacg gaaaatattt gtaaataaga acagttctgg tttcatatat
    25501 tctgtgactt tgctcaaact tgcttcatct atttctagga cagcaaactt tttctgtcct
    25561 ttttttggtt ttgcctgcaa gaaagtcgat acaattccct gtaccatatt cgcaccagta
    25621 gggttggtta gaacttcatc gaattgctgg cgcaaaatat tgactgttaa agcggtagtc
    25681 aatgttttac cgtttgttcc ggtgacaacg acgacttggt agtcttttgc caaactatct
    25741 agtatatgtg gatcgacttt cagcgctagt ttacctggta aacttgatcc gcctttaaaa
    25801 aaagttttta atgcccattg cgacgttttt ccaactgcaa tcgcaaaatg actgcgtatt
    25861 cccattatgt aacctccaaa aacgatttgc tgacgaataa attcaactaa tcataccata
    25921 tgttgaaaaa aaggttaaat tgcttttctt attcttaaga aaaggttacg gctgaaagtg
    25981 catgaaatcg gacaaaatcg catgatttaa caaaaaaaca gcttgaaaca aaatgattgt
    26041 ttcaagctgc tgtgatttta ttattccctc ttacctatat tcccatttta accgataata
    26101 attttctcgc ttggatattc atagcctaga tctcgtgttt cttttggcac gaataacatc
    26161 aatgtatata gcattccgat ccgcccaata aacatcagtg tagcaatcgt gattttgccc
    26221 acaacagaca agtctccggt aatccctaaa gataaaccag ttgttccaaa cgcagaagtt
    26281 acttcgatga tgatcgagat cagcgtttgt tcttctgtag cagataaaaa aacgatacaa
    26341 aagaaacaca taccaagaga aagcataaag acaacgactg attttcgcac atcatcttga
    26401 tcgattctcc gtccaaaaat attgatgtta tcttcacttt tgaggaagga atatagatac
    26461 aaaccgatga ttgctacagt agttgttcga atcccgccgc ctacagaact aggactgcat
    26521 ccgataaaca tcagtaaaga aaaaatgatc agtgttgtga tttgaaaatc acccaaatcg
    26581 tgaatctgca gaccagcatt acgagtcgta atcgaataaa acatcgaatt gatccatttg
    26641 acactcatat tggaatcttg gaataaatgg tccttttcca atagataaat caagacagtt
    26701 cccgaaacaa acaagataac aaaagctaac acagctaatt tggtaaacaa ggaaaaacga
    26761 aacggaagct tcgcattgga acgtttgtat aatagccatt cccggcactc catcaataca
    26821 ggaaaaccaa ttcctccgac aaaaatcaaa aacatgatca ggatcaagaa aaaatagtca
    26881 tgtgcaaagg gcttgatgga atccccagtg acgtcaaacc cagagttcgt gacagccgat
    26941 atcgcctgat aaaaaccgta aaaaacggca tcacgccaac gatcaaaata gcctctgtaa
    27001 taaaaataaa tagaaaagaa tgtcccaaaa agtaattgaa accagattaa aatagcaaaa
    27061 gtgatccgaa tcagtcggac gataccagac aaacgcggtt gattcatatc cgtcatgatc
    27121 agctgtcgct gtttcagtgt gatccgtcgt ttagaaaaaa tgacaaaagc agtagaaatc
    27181 atcatgatcc ccaaaccacc aacttgaaat aatatttcca gcagtataat accgttatca
    27241 ttgaacacag aattgatatc aaaagtcgat agtccagtca cactcactgt actgattgcc
    27301 atgaacagca tgtccacaaa agaaacatgg ctatctggtt cgcgaaaaat cggcatataa
    27361 aatagaacga gtgataagac cgtcatcaat atataataaa aaacgatgat ttggatcgat
    27421 gagaaatgat tggcagcaaa cttctgtcct cgccgctgca gccttcgaaa gaataaatca
    27481 acatgcacag tactatcctc ctgacaatag tcgtttctac cagtatacaa ggggaattag
    27541 gaatactcaa agaaattatc aaacagataa caatacagct ttcgattaaa atagaaatgg
    27601 cttataataa ggaaaatcct agaaaaaaga aggaggagga ataacaaatt gattaatgct
    27661 caacaaatcg tagaaaaaat gaaaaatcaa aaaataaatt atgatacagt tctgaaaaaa
    27721 atgatcatgc aatgggagaa agaagaagta aagcctaaga tattaataca tacatgctgt
    27781 gctccttgca gtacgcacac attggaattc atgtctcaat atgcagagat cacgttattt
    27841 ttttcaaatt cgaatattca tcctaaaagt gagtatttgc gaagattaca tgaacaaaaa
    27901 cgatttgtag accaatttaa tgagaaaact gaccatcatg ttgcgctaat cgttgatgaa
    27961 taccgtccaa atgaattcac caaaatggtt ttaacaaacc atcttgaaaa cgaaaaagaa
    28021 ggtggtgctc gctgcagtgc atgcttcaat atgcgattag atcttgttgc gcaaaaagcg
    28081 caagaactag gatacgatta ctttggaagt gccctgacca tctcgccaaa gaaaaatgca
    28141 gctttgatca atcagatcgg aatggatatc caaaagattt acgggacaca atacttgcca
    28201 agtgatttca agaaaaacaa aggctatgaa cgctctttgc aaatgtgcaa agagtatgat
    28261 atttatcgcc aatgttattg tggttgcgtg ttcgctgcca agcagcaagg atgcgatctg
    28321 aaagaaatca atcgggaagc aaaagcttac ttaaaaacat caaccgtagt ttttgaggaa
    28381 aattaaagaa atctttcgga aaaacgtaag aaaaaaataa agaatttatg gtagaaataa
    28441 ggtagactgt ataatttgtc cgtagtaaag taaggaaaca agaataaatt tcaatgaagg
    28501 aagagataag atgcgaaaag tagcacctat tttgaagcca atcgctgtat taacaatctc
    28561 gacgattgtc gcgactacta caggtattgt gaatagttcc agctaccgtg caccaagttc
    28621 aagttctagt actgtagaag aaacaacacc aactacttca gtctcgacat ctgaaccaac
    28681 aacaacggaa agtttgaatg aaaccactac gacaagtaca tgggacgata caacaactag
    28741 ttcgtcgtat gatgagacat atacaactga cagctcaagc gaaacaagta atacggtacc
    28801 agatggcact ccatcggatt caaccaatcc aagtacaagt ccggatacaa gtgctagctc
    28861 cgaaacgata ccaagcagta cgactgtaga tgataacacg caaaatgatg atgcagcacg
    28921 tacagcacag caaaaccaag attcaggaaa tacccaaacg tataaccagc aagcaggtga
    28981 ttctcaatga catttttact agaccttatc aacggggtgc ataaattcct tggctattta
    29041 gacatcagtc ctaaatatct taaccgtgga tatacgattt taagtgtgat tcccacgctt
    29101 tatttattga gaattgttta tggactttgg caaaaccaaa actatcttca atttttctta
    29161 tatggacttg catttctagt gcttgtttat tttactgttt taaatgtatt ttattacttt
    29221 ttagataaaa acacgaaagc cgatgtcact cagctttttg tgaagtattt acctgatgaa
    29281 gcgttcaaca tccagacaga agaatcgata gataatggaa attcgatcga taaagtgaat
    29341 acacaagagg tcatggtttc ttatgacgaa gattatcaat tgaaattagc cgagaatatg
    29401 cggtatttga ttggaaatgg tgagatcaaa acaaatgaac tcggtcgcat ggatggattt
    29461 ctggtcgata gaaatacatt gtatccttat tactatgtca agaaaacagg agagaaagag
    29521 tatgttctaa atattggacg aagctactcg gacatggaaa aagtagggac cattcgactt
    29581 gactcaacag aagacacatt ggaacctgtc ggattgttca tcgtcggcgg tgattttgtc
    29641 aaagacgggt tacgctatca tgagccttat cgtctgaaat tgattgcaaa aaaggcacag
    29701 tcgcaggaag aaccagtggt ttatagtcgt agccaaagaa gaagacattc ataatgtgat
    29761 gaaacgccgt gtatcagtgg ttgcgataaa aacgtttatt cggaataagg gggatgtgac
    29821 acgtcttttg tcacgttcct tttttatata acgagaaaga taagacgaac acatcactta
    29881 ttctttcctt tcatttataa acattgtaga ataaagaaag tacaacgaag tattgtgaaa
    29941 aaacgtaata aataacgagg aaaatacaag taggaggtaa gctgtgaaag aatttttgaa
    30001 aaattggggg attttgatcc ttgtattagc ggctatttta ttagctagag tgtacgtgtt
    30061 tacaccagtt actgtaaacg ggcattcgat ggaccctaca ttaagtgatg gacaacgatt
    30121 gatttcatct aagatctcaa actatgaacg aatggatatt attactacaa aggaaccggg
    30181 ggatgaagaa cggatgattg ttaaacggat catcggtatg cctggagata cagtcaagat
    30241 ggaaaatgac cagttgacca ttaatggcaa aaagtatgat gaaccttatc tggatgagtt
    30301 caaaaaagaa ttttcagaag ataagttaca aggagaatat gcttatagct caggattcca
    30361 agcacaagca gaaagttcat ccacctttac gagtgacttt gaatatactg tgccaaaagg
    30421 aaagtatctt gttctaggcg ataaccgctt gatttcaaaa gacagccgaa tgtttgggtt
    30481 agtcgacaaa gatatgatcc aaggaaaagt tgttttccgt tattggcctc tttcagaaat
    30541 taaaatcatg tgatgaaaaa ataaaaagaa gctgtgacat gttttttgtc acagcttctt
    30601 tttccgaaaa ttccttctta tttcttggag ttgctcactt tttttgcaat ctcattttaa
    30661 gctttttggc tgatggataa gaaaagcgaa gcgtttttgt tcataaatag cttttcccca
    30721 cgatggaagg tatgactagt agcagagaca aagcccgttt tttccaatcg attattcaat
    30781 tcttcttggc taaagccatt atggactttc ggatgactaa tttgttcatt cttgtcaaag
    30841 tctacgatga tcaattgacc gccaggagct aaaatttcat aaagttttgt aagaatattc
    30901 tccgtatcag gaatgtgtaa aagtacaagt gatagaagga tgagattagc ttggacagca
    30961 ggaaattcga cagaagcatc agcgtggatc gttcggacat ttttcagatc tgctgcttgg
    31021 atcttttctt ctgccatttt cagcatagtt tctgaagcat cggcaatgat cagttctttg
    31081 aatctttccg caagcggtaa actaacaagc ccagttccgc ctccgtaatc cagcaggact
    31141 ttttggtggc tgtcgcgcgg tagataagta gttagttctt cgcgaataat cttggctaat
    31201 gcttgacggt caggggaatc ataatggtta gcgatggaat caaaaatatt ttttgtcatc
    31261 aaatagctcc ttacgtaaaa ctttgaaatt ttaacttatt atagcacgtg aaaagaaaga
    31321 aagcatgcta tactcaattc aaaaagggag aatctagaaa gaatatgccg atatcgtttg
    31381 taaaagatag agaggaaaaa ggaaagtgtg tccgagaaat attactagac ttgcctgagt
    31441 ggtttgggct tcctgaatca acggaaaaat atatcgagga atcaagtaaa ctacctttat
    31501 ggtgtgaaaa aagaaaagaa gaatatttag gctttatcac tctttcacag acaagtgaag
    31561 atactgccga aatctattct attgtatggg aataaaaaaa gactatcatc accaaggatt
    31621 aggaacaaaa ctttttaaag aagcagaagg ttacgcagca aaacattaca aatatttgca
    31681 agtgaagact gtagacgagg ggcactactc tatatatgat caaactattt gtttctacga
    31741 atcattagga ttttcaaggt tggaagtttt tccaaattta tgggatgaat ggaacccatg
    31801 tttagttctt gtcaaaaaat tggaacaaaa gtgagcaata gatttaatac taatctgtgc
    31861 ttgatgaaaa ttctcactgt aaaaaatgct gaaaatcaag tataatacca taggaacaaa
    31921 ctggaaggtg aaaaagagca tggcacaact attttttaag tatggcgcaa tgaatagcgg
    31981 caagacgatc gagatcctaa aagtcgcaca taattatgaa gaacaaaata agccagttgt
    32041 cttaatgaca agcggtattg atacaagaga tggtttaggc gttgtttcaa gccgaatcgg
    32101 actgaaacgt aatgcgatgc cgatatttga agaaacagac gtatttgacg taattcagaa
    32161 aatggaaagc aaaccctatt gtgtgctgat cgatgaagcg caattcttaa aaaagaaaca
    32221 tgtcattgct tttacgagaa ttgtggacga attggatatt ccggttatgg catttggttt
    32281 gaaaaatgat tttcgaaacg agttatttga aggatcaaaa tatttattgc tttatgcaga
    32341 caaaatcgaa gaaatgaaaa caatctgttg gttctgccac aaaaaagcaa tcatgaattt
    32401 acattatatt gatggaaacc ctgtttatga aggcgatcaa gtgcaaattg gcggaaatga
    32461 agcttattac cccgtttgtc gaaaacatta ctttcaccca tcaatgatta cagaaggaga
    32521 ataagcatgt acgatcaatt acaagcaatt gaagaccgtt atgaagagct tggagaactt
    32581 ttaagtgacc cagaagtgat ttctgataca aagcgtttta tgcagctttc aaaagaagaa
    32641 gcaaacacgc gcgaaacagt cgaagtttat cgtcgttata aagaagtagt acaaggaatc
    32701 accgatacag aagaactgct tggtgaaaaa ctggatgatg aaatggctga actagcaaaa
    32761 gaagaattat ctgaattaaa aaaagaaaaa gaagtcttag aagaaaaaat caagatcttg
    32821 cttttaccaa aagatccaaa tgacgataaa aatatcatca tggaaatccg tggagcagca
    32881 ggcggagacg aagcagcatt attcgctggc gatcttttca atatgtacca aaagtatgct
    32941 gaagcacaag gctggaaaac agaagtgatg gaagctaacg ttaccggaat cggtggatat
    33001 aaagaagtga tcatgatgat caccggagac aacgtatatt cgaaattaaa atatgaaagt
    33061 ggtgcgcacc gcgtccagcg tgttccttca actgaatcgc aagggcgtat ccatacttcc
    33121 accgcaacag ttgtcgtgat gccagaagct gaagaagtag agatcgatat tgaagacaaa
    33181 gatatccgta cagatatcta tcatgcttca ggtgctggcg gacagcacgt caataaaaca
    33241 gcatctgctg tacgtttgac tcacttacca acagggatcg ttgttgctat gcaagatgaa
    33301 cgttctcaaa tcaagaaccg tgaaaaagca atgaaaatct tgcgtgctcg tgtgtacgat
    33361 aagatccagc aagaagcaca aagcgagtat gatgccaatc gaaaatcagc agtcggaact
    33421 ggggatcgtt cagaacggat ccggacgtat aatttcccgc aaaatcgtgt gactgatcat
    33481 cgtatcggat taactatcca aaaattagat cagattttag cagggaaatt agatgagatc
    33541 gttgatgcat tgatcatgta tgatcaaaca tcaaaactgg aagctatgca aaatgggtaa
    33601 aatgacctat cgagaagtcc tgtcacgggc ttcttctttt ttagaggatc atgggaaaga
    33661 agcatatagt atccaatttt tatttttgga acgaaaacaa tggaaaaaac ttgattggct
    33721 ccttcatatg aacgaagaga tttcagaaga agaacagcgg ttgatcgaaa ccgatctgcg
    33781 acttctgctt gcagatcatc cacctcagta tttgctagga tatgcagatt tttatgatca
    33841 tcggttaaag gtaactgaag caacactgat ccctcgacca gaaacagagg agttagtcga
    33901 atggtgttta gacgagacgc ctggtgtacc gttagaagtg atagatattg ggacagggac
    33961 tggagcaatt gctattagtt tgaaagcagc aagaaaaaat tggcatatat ctgctgtgga
    34021 tctttcagaa gaagcattag aagtagcgaa agaaaatgct cagaaagaag gaacgaaaat
    34081 cagtttttat catggagata cattaggacc agtaatggat cagcaatttg atgtgattat
    34141 ttctaatccg ccgtacatca gtcggaatga atgggaactg atggatgaaa gtgtccggtc
    34201 attcgaacct aaaatggccc tttttgctga gaacgatgga ttagctattt atgaaaaaat
    34261 agcagttgaa gcaccaagcg tactaaaatc aaatggaaag atttttttag aaatcggatt
    34321 tcgccaaggt gaagcagtaa aaaaaatatt tcagcaagca tttccagata aaaaagtggc
    34381 gataaaaaag gatctttttg gcaatgaacg aatgatccaa gtttatggtt gaagttgtgg
    34441 atgaaaaatt ttttatttgt ttttttatta taaaaaaaca cttatttgac aaggatgtaa
    34501 acgacttttt ggtttatcaa caagtttatc cactattgac aaatagatta tccacaaaca
    34561 gtggaaaact tgaggataaa ctcagacaaa ataagaatgt atgaaaaagg ggttgtggat
    34621 aaactgtgga aacgaaacga tatgatgaaa cagaattgca agaggcagcc gaagcactga
    34681 aagaaggaga acttgttgct tttcctaccg aaacagtata cggattgggc gccaacgctc
    34741 ttttaccaaa cgctgtcaag aaagtgtttt ctgtaaaagg gagaccgcaa gataatccat
    34801 taatcgtcca tgtcgcctct tttgaacaag taaaagagta tgtggacaac tttcatccgg
    34861 cagcagagaa gattgtgaag aacttctggc ctggaccatt aactttgata tttaagatca
    34921 aaaaagacac tttgccctca gttgtgacgg gaggattatc cacagctgca tttcgtatac
    34981 cggataacaa aaaaacacta gaagtaatcg aactttccgg tgttccgctt gtcggtccaa
    35041 gtgcaaatac gtcgggaaaa ccaagtccaa cgactgcaga tcatgtatat catgatctac
    35101 aaggaaaaat taccgggatt attgatgatg gagctacgcg aattggtgta gaatccactg
    35161 ttcttgattt aagtgatcca acagccatgc ctatgatttt acgaccaggt gcagtaacga
    35221 aagaacaaat cgaagcagtc atcgaatctc ctgttgctat tgatcagcat cttgtaaaag
    35281 aaaatgaaac accaaaagcc cctgggatga aatacaaaca ctactcaccc gatacacgtg
    35341 ttctgatggt cagagatggc gattggtcaa ctgcagttca atgggcaaaa aataagaaga
    35401 tccgtgcagg tgtcatagca agccccgaaa ttgcggatca agtgcggaca gatactgcag
    35461 ctgtttatat gtataacgac aactcagtag aagcggcggc taaaggattg tttgcaggtc
    35521 taagaggatt agatgaacca acacttggat tagatttgat ttttgtacaa gtgtatccag
    35581 aaactggatt ggggaatgct tacatgaatc gcttgaaaaa agcagcagga caaaattatt
    35641 ttgaaaaatg atcaattggt tattgtcgca ttcttttagt atgatacaat gctatcaagt
    35701 aaatcttcta tcagtgaacg aaaaactaag cgtaactaat cgtaatagaa gagatacgaa
    35761 tatttggcaa tttgtttttg atgggaaaaa taaaattgta ggaaaataaa aaggagctgt
    35821 tgtttgtggt agattacaaa acgtttgacc ctgatctttg ggctgcgatc gccaaagaag
    35881 aagaaagaca agaacataat ttggagctga ttgcctctga aaactttgtc tccgaagcag
    35941 tgatggctgc acaaggaagt atcttgacaa ataaatacgc agaaggttat ccagggcatc
    36001 gctattatgg tgggtgtgaa tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa
    36061 aagaattatt cggagcgaaa tttgcgaacg tccagcctca ctccggctct caagccaaca
    36121 cggctgctta tctcgctttg gtagaaccag gggacacgat cttaggaatg gatctttccg
    36181 caggcggaca tctaacacat ggatcaccag tcaacttcag tggcaaaacc tatcactttg
    36241 ttgcttacgg tgttgatccg acaacagaag ttattgatta taatgtggta cgtattttag
    36301 ctagaaaaca tcaaccaaaa ttgatcgtag caggtgccag tgcttacgga cgaacgatcg
    36361 actttgcgaa attcagagaa atcgcagatg aagtaggcgc aaaactgatg gtagatatgg
    36421 cacatatcgc aggattagta gcagcaggac ttcatcctaa tccagttcct tatgcagata
    36481 tcacaacgac taccactcat aaaacattgc gtggccctcg aggcggcatg attttgacaa
    36541 acgatgaagc attagcaaaa aaaataaata gtgcggtatt tcctggtatt caaggcggcc
    36601 ctctagagca tgtgattgcg ggtaaagcag tagcattcaa agaagcatta gatcctgcat
    36661 ttaaagaata tagtgaacaa atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc
    36721 aagcaattgg aaccagagtc atttcagggg cgacagataa tcatctgatg ttgatcgatg
    36781 ttcgcgagtt aggtataaat ggaaaagaag cagagagcat tttggacagt gtgaatatca
    36841 ctgtaaataa aaactcgatt ccttttgaaa cactaagtcc attcaaaaca agcggtattc
    36901 gaatcggcac gccagcaatc actactcgcg ggttcaaaga agaagatgct gtaaaagtgg
    36961 ctgaactagt tgtcaaagca ttacaagcaa aagatgataa cgctcaacta gacgaagtca
    37021 aaacaggtgt acgcgaatta actgaaaaat ttccgttgca taaaaaataa aaatttaaat
    37081 tccattagta aacagaaaag ttcaatgttg ggcttttctg tttatttttt gggaaaacgg
    37141 attctttgac aatatcggtg atgctctcta gtatatgagg tgtaatttta gtcttcacac
    37201 taaatctcag ggaggaaaag tcatgactga tcgtatctcg ttagaaaagg aagcagtagc
    37261 attcagtgat gcaaatgcgc ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag
    37321 agttttacta gaagaagtac aagaatcacc tgtgaacaaa tattccgtcg atattgaaga
    37381 cttgatggtg gatactggcg aatggggaaa actcaatatt cgtctcgtac gaccagaagg
    37441 gaatatggat aaattaccag ttatttttta tattcatgga gcaggctggg tttttggcag
    37501 tgctcaaacc catgataaat tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt
    37561 ctttccagaa tatagtcgat caccagaagc aaaatatcca acagcaattg aacaaaatta
    37621 tgctgtgttg caacagctga ataaattagc caagtcaaaa aatctggatc tcgagaatct
    37681 aatcgttgca ggagattcag taggtggtaa catggctaca gtcatgacga tcttaacaaa
    37741 agaacgaaag ggcctcccaa tatccaagca attgctgtat tatccagtca caaacgctga
    37801 ttttgataca gattcctatc atgaattcgg agaaaactat tttttgacaa gagaaggaat
    37861 gatgtggttt tgggaccaat atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc
    37921 gcctttgaga gcaaatataa atgaactaaa tgatcttcct tcagcaatga ttttaacggg
    37981 agaagcggat gtattaagag atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg
    38041 ggttcctgtt acacaagtac gcttccaagg aatcatccat gattttgtca tggtaaatgc
    38101 tatggatcag acaaaggcaa cgcgtgcagc aatggcatta tctacttctt ggatcaagaa
    38161 aaaagaataa gctttgcttg tccgcaggaa aacttctttt caaaaacgga ttttcttagg
    38221 aaaaatggcg agtttgacta gtcaatcact tgattttgtt ttacaattaa aaagaaacga
    38281 cagaacaaag gagagcttaa aaaatgggca aatttcaagt gatcgatcat ccattgattc
    38341 aacacaaact aactattatt cgggacaaaa actgcggaac aaaggttttt cgtgaagtag
    38401 tcgatgaaat cgctatgttg atggcatatg aagtatcacg ggatatgcca ttggaagaca
    38461 tcgtgatcga aacaccgatc actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag
    38521 cgattattcc aatcttgcgc gcaggtattg gaatggttga cggtatcttg gaattgattc
    38581 cagctgctaa agtgggacat atcggtcttt atcgagatga agaaacattg gaaccacatg
    38641 aatatttcgt caaacttcca gaagacattg atgcacgtca gttgtttgta gtagatccaa
    38701 tgttggcaac aggcggatca gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt
    38761 cgaacattaa atttgtttgt ttggtagcag cacctgaggg agtaaaagct ttgcaagaag
    38821 cgcatcctga tattgacatc tatacagctt ctctagatga aaaattgaat gaacatggtt
    38881 atatcgttcc tggactaggg gatgcgggtg accgtttgtt tggtacaaaa taagtaaata
    38941 cgaaaaaggg tgtgacacaa cttttgtcac atcctttttt ggtcattttt aacgtttgat
    39001 gcagaacagc tttttcacag ctttcgtttg catataaaaa aaccatccca aaaatgcaga
    39061 gatggtcaaa ttcaaatctt tttttagttc tttgtagtta acgattacta cacccttact
    39121 ttgcgtcatc aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt
    39181 caattgcttt gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt
    39241 attgatcttg aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa
    39301 agttgccatt ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata
    39361 tcatgaaatt aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa
    39421 tttcttttgg ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag
    39481 gtcgtaggtt cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt
    39541 taatccattg atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat
    39601 agagcatgta ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg
    39661 aaatcaaata tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct
    39721 tggttgaata acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa
    39781 atatttccat tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa
    39841 ctagcgacag gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat
    39901 tcttctccaa gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt
    39961 attggattgc ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca
    40021 ctgattacta cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc
    40081 gagaacattt ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg
    40141 atagttaaag aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt
    40201 acaacgacaa tagtgcccaa tatccctttt ttaagttcta ttttttttcc taaaatgtta
    40261 ttcatgataa taactcctac tattcagttt ttgaagatag agtcagtata catgaagagt
    40321 taaaataggc aatgtttttt tatggatatt tgtattgcta aaaatttatc ttatatattc
    40381 attttgatta tactactttt atttgtaaaa attttctagc aaaaccaagt gcttgcaact
    40441 gattaagcgt acaatttttt agatgttccg agcgtacttc aatcatttca aaggagttgc
    40501 tacttaaatc gatatttttt aaatcagtat caaaaaagct agcattttct aaatcattag
    40561 tagaaaacat tattttagtg agggaacagt ctacaaaatc tgcgcttgat aagacagagt
    40621 gctcaaaggt actttctttt atgtttgaac cagataaatt tacatagtcc attttacaat
    40681 tctcgaataa cgtgttccca atataacttt ccatcaattt agtacctgtt aatttacaat
    40741 tgacaaattt cacgcgatgc aacatgcttt ttgtcaatat acagcctgaa aaatcacagt
    40801 tttcgaatat acagtcggta atctccattc tttgaaaatc attggaagca aaaaatgcat
    40861 acttgaaaat agatttgtaa ataattggat ggcagattat ttggttttcg atgacctctt
    40921 tttgtgaaag gatattatac agttcaaaat cttcgttttc atagatatga aaaaaggatc
    40981 ctttattcaa gcttgcttca ttaactttag gaaaaatcat atttaattac ctccattttc
    41041 ataaagtcat attaacaaaa gctaaaccaa cttacattag cagaaaatga gataggtttt
    41101 cgcctttttc gataacgata gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag
    41161 agacacaaat taggcgtaat tctaataaaa atcttagaat aaaaattttt actgatttaa
    41221 tttagaatgt ttttaaaata aaaatcgttg ccataacagt gtattgaaag gttttaacaa
    41281 tccagtaatg atttaaaaaa atctagatgt agcgtatact ttgtttatag aatgtagtgg
    41341 gaagaaatat atttttggga ggaaatcgtg atgtctaaaa gtgaagaaat agaaaacatg
    41401 gtactaaaat tatatgatct cgttttaaat ccagagatca aagaaaagga acgtatactt
    41461 ttgatagacg caaagacagg cttagaaaaa ggtcagtatt atcctaaagt cataaataat
    41521 ttggaacgta gtttacgtcc gttagcaatc aggggagaat tgtctaaacc agtatcaccg
    41581 ttttatatgg agatatcaac aataggaaaa tttgaaaaag aattaggtcg aggaatggct
    41641 tcggcaccga ttacttttgg gcatttataa aaacgagtta ttcatctcaa tgaattaaaa
    41701 agtatagtca atctcataat gcaggctagg ttcatttatt tgaacagagt ctgtattttt
    41761 ttattctaaa taaagaaaca gatgtttctt tatattgtat tttatccttt aattaacaca
    41821 aaaacagcta gtataaacta ataatgttaa ttaaaagtgt atttt
//
LOCUS       DAJSVF010000020        37101 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSVF010000020 DAJSVF010000000
VERSION     DAJSVF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.020"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(159..443)
                     /locus_tag="ON550_001011"
     CDS             complement(159..443)
                     /locus_tag="ON550_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954109.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(461..1351)
                     /locus_tag="ON550_001012"
     CDS             complement(461..1351)
                     /locus_tag="ON550_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR3954110.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1371..1589)
                     /locus_tag="ON550_001013"
     CDS             complement(1371..1589)
                     /locus_tag="ON550_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR3954111.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1559..3259)
                     /locus_tag="ON550_001014"
     CDS             complement(1559..3259)
                     /locus_tag="ON550_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR3954112.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3526..4299
                     /locus_tag="ON550_001015"
     CDS             3526..4299
                     /locus_tag="ON550_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954113.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="ON550_001016"
     CDS             complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="ON550_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR3954114.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5015..5117)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5123..6094)
                     /locus_tag="ON550_001017"
     CDS             complement(5123..6094)
                     /locus_tag="ON550_001017"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3954115.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="ON550_001018"
     CDS             complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="ON550_001018"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR3954116.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8064..8882)
                     /gene="purR"
                     /locus_tag="ON550_001019"
     CDS             complement(8064..8882)
                     /gene="purR"
                     /locus_tag="ON550_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR3954117.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9049..9873)
                     /locus_tag="ON550_001020"
     CDS             complement(9049..9873)
                     /locus_tag="ON550_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3954118.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9846..10526)
                     /locus_tag="ON550_001021"
     CDS             complement(9846..10526)
                     /locus_tag="ON550_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3954119.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10549..11487)
                     /locus_tag="ON550_001022"
     CDS             complement(10549..11487)
                     /locus_tag="ON550_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3954120.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11732..12595)
                     /locus_tag="ON550_001023"
     CDS             complement(11732..12595)
                     /locus_tag="ON550_001023"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR3954121.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="ON550_001024"
     CDS             complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="ON550_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3954122.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15451..16257
                     /gene="proC"
                     /locus_tag="ON550_001025"
     CDS             15451..16257
                     /gene="proC"
                     /locus_tag="ON550_001025"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR3954123.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16336..17892)
                     /gene="rny"
                     /locus_tag="ON550_001026"
     CDS             complement(16336..17892)
                     /gene="rny"
                     /locus_tag="ON550_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR3954124.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18303..19352)
                     /gene="recA"
                     /locus_tag="ON550_001027"
     CDS             complement(18303..19352)
                     /gene="recA"
                     /locus_tag="ON550_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR3954125.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19461..20702)
                     /locus_tag="ON550_001028"
     CDS             complement(19461..20702)
                     /locus_tag="ON550_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR3954126.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="ON550_001029"
     CDS             complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="ON550_001029"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR3954127.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21729..22652)
                     /locus_tag="ON550_001030"
     CDS             complement(21729..22652)
                     /locus_tag="ON550_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3954128.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23391..24677)
                     /locus_tag="ON550_001031"
     CDS             complement(23391..24677)
                     /locus_tag="ON550_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3954129.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24670..25938)
                     /locus_tag="ON550_001032"
     CDS             complement(24670..25938)
                     /locus_tag="ON550_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3954130.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26190..26651
                     /gene="mscL"
                     /locus_tag="ON550_001033"
     CDS             26190..26651
                     /gene="mscL"
                     /locus_tag="ON550_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR3954131.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26710..27156)
                     /locus_tag="ON550_001034"
     CDS             complement(26710..27156)
                     /locus_tag="ON550_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954132.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27249..27494)
                     /locus_tag="ON550_001035"
     CDS             complement(27249..27494)
                     /locus_tag="ON550_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR3954133.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27609..28187)
                     /locus_tag="ON550_001036"
     CDS             complement(27609..28187)
                     /locus_tag="ON550_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3954134.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28264..28794)
                     /locus_tag="ON550_001037"
     CDS             complement(28264..28794)
                     /locus_tag="ON550_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR3954135.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28798..29658)
                     /gene="rlmB"
                     /locus_tag="ON550_001038"
     CDS             complement(28798..29658)
                     /gene="rlmB"
                     /locus_tag="ON550_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR3954136.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29651..30046)
                     /locus_tag="ON550_001039"
     CDS             complement(29651..30046)
                     /locus_tag="ON550_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR3954137.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30043..31455)
                     /locus_tag="ON550_001040"
     CDS             complement(30043..31455)
                     /locus_tag="ON550_001040"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR3954138.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31452..31988)
                     /gene="cysE"
                     /locus_tag="ON550_001041"
     CDS             complement(31452..31988)
                     /gene="cysE"
                     /locus_tag="ON550_001041"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR3954139.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(32074..32313)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32310..33770)
                     /locus_tag="ON550_001042"
     CDS             complement(32310..33770)
                     /locus_tag="ON550_001042"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR3954140.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33940..35097)
                     /locus_tag="ON550_001043"
     CDS             complement(33940..35097)
                     /locus_tag="ON550_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR3954141.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35131..36501)
                     /gene="radA"
                     /locus_tag="ON550_001044"
     CDS             complement(35131..36501)
                     /gene="radA"
                     /locus_tag="ON550_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR3954142.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36564..37091)
                     /locus_tag="ON550_001045"
     CDS             complement(36564..37091)
                     /locus_tag="ON550_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR3954143.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        10248 a         8086 c         6507 g        12260 t
ORIGIN      
        1 ataccgagaa aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga
       61 attttgtcac aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat
      121 tctgattatt tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa
      181 gtattcacta cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct
      241 acctcttctg tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg
      301 ttgatcaaaa taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca
      361 aagccgtttt tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg
      421 aacgttacgt cataatattt cattatctct acctctttcg tcattcaata ttttgtactt
      481 gttctctgat tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg
      541 catttgattt ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta
      601 attcccgacc tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg
      661 ccagacgatc cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca
      721 acaaccgttg ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta
      781 tttgaaattc cttttcatag atttctacaa aactatcgag tttttttagt acttctttga
      841 attcttcagt attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg
      901 cctcaataga atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca
      961 tgctctcttc cggtttttct tctaccgtta cgaagttttc gtccagcgcg aagcgttccg
     1021 ccatccgact aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca
     1081 attgagtgat aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc
     1141 caatataagc tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa
     1201 gctgacgaaa atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta
     1261 gaaaacgctg gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga
     1321 tggctttgcc aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt
     1381 tctattgctt tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt
     1441 ttttcatggt ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg
     1501 actaggttca gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct
     1561 acgatttcat atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata
     1621 ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt
     1681 ttccttgtag cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct
     1741 ggtattttcg ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat
     1801 catccggcat actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat
     1861 agatttcatt gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc
     1921 atgtctctgg tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc
     1981 ttttgaaaaa agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc
     2041 agtatgttct tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta
     2101 atcgtttttt catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa
     2161 aaagcagaaa gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg
     2221 gcatacgaat ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg
     2281 ctcctttgtt aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt
     2341 tattttttcc gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc
     2401 aatctggtat ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg
     2461 gcagtttagt agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat
     2521 ccaaattgtc tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg
     2581 ctgttttcgt catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag
     2641 ccatttctgg taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt
     2701 ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga
     2761 taatctcaaa gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac
     2821 cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt
     2881 gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag
     2941 attgaaataa aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc
     3001 gaaaaaattt tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat
     3061 ctcctctact tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag
     3121 cacattgctt ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa
     3181 atggaagggg ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt
     3241 aaactttttt tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa
     3301 aaggtcatga gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct
     3361 cctttaaata aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat
     3421 gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat
     3481 gttgtaaaat aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt
     3541 tatgtacata ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac
     3601 tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct
     3661 gctggagaat atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag
     3721 cgtttctttt cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt
     3781 actgatttaa cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt
     3841 ggtcatatga aaacacatct tcattcgccg tttttttata aactacagaa taattttgta
     3901 tacttcgatc ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat
     3961 aggatttttg ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct
     4021 tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt
     4081 aatttgatgc aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa
     4141 tatcgtatcc caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat
     4201 cgagaagagg aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg
     4261 gtagaagatg ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat
     4321 aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt
     4381 tacagctcct gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat
     4441 aaaacgatga tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa
     4501 aagagccaag gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata
     4561 aaatgccaaa aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta
     4621 atttgtttcg tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct
     4681 gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa
     4741 taaacttgtc ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc
     4801 tttgtccctc ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa
     4861 ctgctgccga tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt
     4921 ccttctaccc aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc
     4981 acaaaggagg aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc
     5041 tcgcataacc gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga
     5101 ctcccctttg tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt
     5161 tgttttcatg gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga
     5221 ttttgtcgat tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga
     5281 tagctgaatc ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag
     5341 catacacact agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg
     5401 ctgtatcgat catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat
     5461 tcattacttc tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt
     5521 tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga
     5581 caacatcatc gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca
     5641 tcaaatgatc gactggaata tcaaagaaac cttggatttg aactgcatga aggtccaaag
     5701 tcagcattct tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg
     5761 gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt
     5821 tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt
     5881 tatcattgac tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt
     5941 cttcaatgtt tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact
     6001 cgacgccgac tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa
     6061 tttttaatct tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa
     6121 ctctagaatt ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc
     6181 aaaagtctta gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg
     6241 ctctcgcaat tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa
     6301 tcgaactgtt tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc
     6361 tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac
     6421 cacatccgac attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga
     6481 ctttagttcc tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt
     6541 tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct
     6601 tgacatggct gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt
     6661 ctacgatttt tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga
     6721 tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg
     6781 tcgttgctga atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc
     6841 tcatgatctc atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa
     6901 aatcatccgt ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat
     6961 ctgttagata gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca
     7021 aagcttcgtt gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg
     7081 gtgttgcatc tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc
     7141 gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc
     7201 cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac
     7261 tgataactaa cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat
     7321 gtcctgttcc caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt
     7381 taacttgttc tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga
     7441 cccgattaat gatatgttct accataggtt taccagaaac gggatgtaaa actttatata
     7501 attttgactt catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca
     7561 actgcgtcac tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt
     7621 caacaaaaca agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc
     7681 ataacgggcc tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca
     7741 cactactcat aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa
     7801 acggtaggct tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc
     7861 tcaataaacg gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca
     7921 ggacgctgat ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa
     7981 gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa
     8041 ctaagcagct ttttcacaac ctcttattct gaaaaataat ttcctggcac aaccgtaata
     8101 tttttcgttt gagtatctac atctttgaca taaagcagtg acgtataatc atcgatggcg
     8161 cgatgaccgt tgaattttga ttcggcaaat acagtgatgc caacaagttc tgattcgaat
     8221 tcttcaatca agctcttcat tccgttgact gttccgccgc ctttcatgaa atcatccact
     8281 acaaggacac gtgaaccacg tttcaaactt ctcttagaca gttccatctt ttcaattctt
     8341 tcagaagatc cagacacata gttgacactt actgttgatc cttctgttat ttttgagtcc
     8401 cggcgtacaa taacgaatgg cgcattcaga taataggaca cagcttgggc aataggtacg
     8461 ccttttgtgg ctacagtcat tacagcatcg attttttcac caaggtattt agaagcaatg
     8521 attcttccaa cttgacgcag cagttcaggt tctcctaaca aatcagagag ataaacatat
     8581 cctcctggaa gcagacggtc ttgttctgat agacgttctg ctagagattc aatatatttt
     8641 ttcgcctctt catatgaaat ttcagggatg aacaatacac cacctgcagc acccgggatt
     8701 gtttccaagg ttccataccc tcgttcttta aatgttttct tgatgattcc caaatcttca
     8761 ctgatagaag atttagcaga ttcataacgt tcggcaaagc ttgttaagga aatcaattca
     8821 tgcgggtgat tcaataaata ctgtgtcatg tcaactagac gttcactgcg acgtactttc
     8881 acctattcca actcctttta agaaatttta tcgttttaca ttatataagt ttttcttcaa
     8941 aaattcgagc attttatgaa aaaataagaa aatagttcgg tatttattaa aataaaaaga
     9001 agcctataaa aaatttttcg tttttataag gcttcttttc tttataattt agttattttt
     9061 tcttctgaag gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa
     9121 gactaatgtg attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga
     9181 cagcatccct agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc
     9241 taatcgcata ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc
     9301 aatcgggatc atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa
     9361 atgaacgggt agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg
     9421 ccgtttgaat aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac
     9481 ttgctcccat gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga
     9541 attccctccg ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat
     9601 ggcaatggag atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc
     9661 tgctaataca acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa
     9721 cgctacccct gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat
     9781 cactaaaaac acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc
     9841 tcttctcatg aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg
     9901 atctgtctgt ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat
     9961 ttcccatgga catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca
    10021 tccatacctg ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc
    10081 gctaaactga ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata
    10141 tcccacatcc ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga
    10201 cgcttgaacc agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta
    10261 ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt
    10321 ccttcttgat ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac
    10381 gatgctttca ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca
    10441 cccgcatcta catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc
    10501 aaatcagata tttcgatata acgcacctta caacctccat tctaaaactt aattgatact
    10561 ttcttttaaa gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat
    10621 ttgttcatta gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt
    10681 cgacaatgtc tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt
    10741 gtttttcttc acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg
    10801 tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc
    10861 agcatgctga gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg
    10921 atccagtgct tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt
    10981 atctggatat ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac
    11041 cggatcgagc caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc
    11101 ttcatgttcg tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat
    11161 caaggtctgt ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga
    11221 actattatac acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc
    11281 gaaatcatgc ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac
    11341 gatctctttt gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt
    11401 tttgtctgct tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac
    11461 cgtagctgtc aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt
    11521 ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc
    11581 atcaatttct gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg
    11641 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
    11701 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
    11761 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
    11821 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
    11881 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
    11941 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
    12001 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
    12061 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
    12121 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
    12181 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
    12241 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
    12301 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
    12361 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
    12421 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
    12481 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
    12541 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
    12601 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
    12661 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
    12721 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
    12781 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
    12841 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
    12901 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
    12961 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
    13021 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
    13081 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
    13141 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
    13201 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
    13261 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
    13321 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
    13381 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
    13441 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
    13501 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
    13561 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
    13621 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
    13681 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
    13741 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
    13801 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
    13861 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
    13921 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
    13981 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
    14041 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
    14101 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
    14161 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
    14221 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
    14281 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
    14341 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
    14401 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
    14461 ctactgctac atcttgccga acaggcaatt gactcattcc taagatcatc aaaacgtcaa
    14521 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
    14581 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
    14641 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
    14701 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
    14761 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
    14821 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
    14881 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
    14941 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
    15001 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
    15061 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
    15121 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
    15181 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
    15241 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
    15301 tatttttcac attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc
    15361 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
    15421 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
    15481 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
    15541 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
    15601 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
    15661 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
    15721 atttctattg cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag
    15781 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
    15841 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
    15901 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
    15961 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
    16021 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
    16081 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
    16141 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
    16201 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
    16261 tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
    16321 cttttgtatt ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac
    16381 tttgatgtgt ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa
    16441 acgaacagcg tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc
    16501 agcttgaact gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact
    16561 tcttaaacga cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga
    16621 gagcgcatct gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg
    16681 atgagaagcg atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc
    16741 accgatctca acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag
    16801 caacccggca cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc
    16861 taattttgca acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg
    16921 cagacgtccg atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc
    16981 tgcctcttcc ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat
    17041 ttcttcgata cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg
    17101 tgcgatttct cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc
    17161 gatgattaaa tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc
    17221 aatgatacga cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga
    17281 aacttgatct gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc
    17341 tgcttcttct ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag
    17401 ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat
    17461 tttttctagt tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc
    17521 aattaattgc tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt
    17581 ttctaacgag tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg
    17641 agattttaat tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg
    17701 attttcttcc ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc
    17761 actatcgatg attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga
    17821 ctttgcgacc ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat
    17881 gttgaatacc ataaaatcca cctccatact atctttttca tttttatcat ctatacagct
    17941 tctatgataa attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa
    18001 catgcacaac tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa
    18061 acggctttct acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat
    18121 aggtaaacct ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata
    18181 tttttatttt atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa
    18241 aaaacggcag gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat
    18301 ttttattctt ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact
    18361 tcttcaccaa taccataagc tgctctaact agagcagaaa cttctgccat catttcagga
    18421 tgattagcca tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa
    18481 gaataccaag caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct
    18541 ccttcttgtg agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt
    18601 gctactttgt tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt
    18661 ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt
    18721 cctccaggag tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg
    18781 atgaaaatag cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga
    18841 gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct
    18901 cgtggaacta aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact
    18961 aaagcatcag cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca
    19021 tcgatattca cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg
    19081 aaagcggccg ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt
    19141 ttacctgaac tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca
    19201 cctaaagcga catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt
    19261 ttttcgccca atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca
    19321 gcatctaagg ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat
    19381 cagagcaaca tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac
    19441 cgaacaaata ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct
    19501 tgcataaccg cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa
    19561 accacgccgt ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac
    19621 gtgtccggtc cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt
    19681 gcttgaatgg ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc
    19741 tccggatcaa tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct
    19801 ggaaatactt cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc
    19861 aaactttccg ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt
    19921 gctagcgaat tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt
    19981 tcttctaatg ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt
    20041 cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca
    20101 atcagatctt ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact
    20161 ttagaaatta gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc
    20221 acaaacattg gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct
    20281 gtttgataaa aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg
    20341 ataatctggg actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat
    20401 gccaataatt ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca
    20461 gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt
    20521 tcacttcttg tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca
    20581 actgtttgat aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca
    20641 ttcgtattca cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc
    20701 attacttccc gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag
    20761 agggcaagga gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc
    20821 tcctgccaaa acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg
    20881 aaattatccc ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata
    20941 cttacattga tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga
    21001 tagtaaagat cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga
    21061 atgggatatt attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt
    21121 ttcccggcca agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg
    21181 tgacggctaa ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt
    21241 caaccaacat gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact
    21301 tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga
    21361 tactagctaa agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc
    21421 ctacgttaaa ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca
    21481 tacaaattct aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc
    21541 ctattctacg tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt
    21601 tattcaaagg agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga
    21661 cacagatttg tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat
    21721 ttatacgatc attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt
    21781 tcccctggat taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca
    21841 ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt
    21901 gctgccagtg tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga
    21961 ttgacagcag taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg
    22021 gcactgccag aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt
    22081 gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc
    22141 tcgactgatc cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga
    22201 cgatcttgcc aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat
    22261 aaaatgacag gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct
    22321 tttctagaag tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg
    22381 cttttcccat caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat
    22441 tgccgaataa atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg
    22501 attgcttcca aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt
    22561 tttttattca ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca
    22621 tcttctccag tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt
    22681 tgggtgctgt gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct
    22741 gccaactcct cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact
    22801 tgttccttgt gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca
    22861 atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt
    22921 atgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt
    22981 caaaagcttg catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt
    23041 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg
    23101 tgttccagaa gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat
    23161 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta
    23221 cttcgtttga ttttatttta atataagttg attaaaagag agcatgattt gttttatttt
    23281 ctcactaatt tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc
    23341 tactacaagt ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt
    23401 ctcatgtaaa actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg
    23461 tctttcattt ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat
    23521 aaactttgcg taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt
    23581 ccaagcattt tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg
    23641 aatccaagaa tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg
    23701 ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg
    23761 ataattcctt gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg
    23821 aacaacagat tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg
    23881 tctaatcctt taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt
    23941 tgtttgatga tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg
    24001 aaattttttg cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta
    24061 ccaactacaa ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag
    24121 tcttgagctg tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg
    24181 tgtgtaggat acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca
    24241 tacatttgga tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag
    24301 taaggtgctt gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta
    24361 gaaaacagat agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc
    24421 ccaaataatt gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg
    24481 gcaattccat ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg
    24541 atcgaaccat aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac
    24601 aaatacacgg tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca
    24661 tattctgttt tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat
    24721 ttgctcagct acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc
    24781 ttcatcagat aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc
    24841 ttgcggagag tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag
    24901 ttccaactct gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag
    24961 tacttgattt cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt
    25021 atccacgcta ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag
    25081 tttagagtga ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg
    25141 ttcatcgtaa tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc
    25201 acgtacttgt cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata
    25261 aactgtttcc cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc
    25321 aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga
    25381 cgctagcgaa ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt
    25441 ttgttccggg ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt
    25501 atcttccttc aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa
    25561 ctgatcaaac tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac
    25621 cgcttgagaa aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc
    25681 gtttagccaa tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag
    25741 ctctgcaagt tgcgcactta actttgtctg atccggataa ttcaaactat ttgtttccaa
    25801 catgctgctg agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg
    25861 gataaaaatc cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc
    25921 tgccaattga tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt
    25981 ttaatgggtc aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca
    26041 attcgccgtt ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca
    26101 aaagaaacag acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta
    26161 aagacaaata aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc
    26221 gtggagatgt cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg
    26281 taaaccaagt ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca
    26341 caggtacttc tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg
    26401 tcacatttgc gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg
    26461 tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc
    26521 aagtggaagt ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc
    26581 ttgccaaaca agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata
    26641 cttctaaata ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt
    26701 tttagattct taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc
    26761 caaatacaca tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc
    26821 ttgtgcgccg atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc
    26881 ttgaacaatc tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc
    26941 aaaatggata ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt
    27001 atcttgaaaa aacgtatgcc aagcttgtcc atcaatgtca tcaacttctt gataaggaca
    27061 ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt
    27121 tgacaattct tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt
    27181 attcttagca taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg
    27241 tttgcttttt aaactgcttc agttaaaaat tcgatctcaa ctgtccgagt aagtacatct
    27301 gaatagctgt aagatactcg ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat
    27361 actgaaggat aggtttctgt taaaacgccg cggcgttctg tttgacgctt tcttcctgtc
    27421 tgagcaacca acatgattgg gctgccgata cgaccttcta aatctttctt gattgaagct
    27481 aaagttgttg gcatacagtt cacctctact atcaactata ccacatattt ttaaaatttt
    27541 caagtttacc ataaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt
    27601 tttctaaatt attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc
    27661 ccgtaagcgc tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa
    27721 tcaacgcctt gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg
    27781 caattttcga ttgcttcttt taataaaaga tgctgaatcg ctaaaggttc ttgcaatgtg
    27841 atctctgcta aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct
    27901 ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt
    27961 ataatgcttt tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat
    28021 gcctgatata aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa
    28081 taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat
    28141 gcttcttcgt caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc
    28201 cataaattcg tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat
    28261 atgttattta ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc
    28321 ttcattccat ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc
    28381 tattgttttt tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc
    28441 tccttgggaa aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt
    28501 caaacggtca ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc
    28561 tttcgtaaaa acaacatcca actggtattt cttataattt tgttggatgc ccggaactag
    28621 ttgggcatcg aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg
    28681 atgtaaaaga gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc
    28741 cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta
    28801 ctccccctga cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt
    28861 taagctctgg acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa
    28921 tcctggactc attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt
    28981 ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt
    29041 gagctgttgc acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc
    29101 cccagtggaa gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat
    29161 cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc
    29221 ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat
    29281 cagttggtca agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac
    29341 gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg
    29401 ttcacgtgcg atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa
    29461 gagtttattc cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa
    29521 attatcttct aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt
    29581 tttatttgaa tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt
    29641 tgttctttct ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt
    29701 ccatccgctc ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg
    29761 cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc
    29821 ctctgcgata ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa
    29881 tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg
    29941 gttttgtttc tccttgatca atcaggtggt cacgaacata gatttcgtaa atcgcatctc
    30001 caacataagc caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca
    30061 tcgggttcct tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat
    30121 ttcgtcgctt cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg
    30181 ttcaatctct tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca
    30241 ttgcgagaat aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag
    30301 ataagtgttc agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa
    30361 atcatcgtcc atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc
    30421 cgcatcatct gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt
    30481 attcttcagt cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat
    30541 cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt
    30601 gatcatttca tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc
    30661 gccaatcgtt acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc
    30721 ttcactttgt gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg
    30781 gatatcgatc gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc
    30841 gggacgtcct ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca
    30901 taaagcgaaa tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc
    30961 tccgacttcc agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg
    31021 ggtacggtaa tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg
    31081 aataaatgct aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggctc
    31141 gacattcaac gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg
    31201 tgctgtaata cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt
    31261 cgatacgaaa tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc
    31321 aatggtgcta cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata
    31381 cataccgaca tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt
    31441 gtagattttt atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct
    31501 ggtactccta ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg
    31561 attttggcgt tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg
    31621 gctccttgtt taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt
    31681 acgccatgga ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc
    31741 attccatggt cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca
    31801 gttaggaaac gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat
    31861 aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc
    31921 tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac
    31981 caactcatga ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa
    32041 agaaaagaag cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca
    32101 cttttcttca taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta
    32161 ctttgtttcc tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga
    32221 ggcatttcgg caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc
    32281 agctgcttac ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat
    32341 gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct
    32401 gatacagcta cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga
    32461 acagctttga tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt
    32521 gctttgaatg cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct
    32581 tcattcaata caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca
    32641 tagctcattt gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca
    32701 tctgggtttt ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat
    32761 gcatctagat ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg
    32821 aaagctgcgg gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt
    32881 tcgaagattt cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt
    32941 gcatctggta gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca
    33001 cgtttgctta gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt
    33061 ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg
    33121 tcatctccac gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa
    33181 ttataagttg gcataccatc gcgtttttgt atgacaaagt cgccaccgat attatcggat
    33241 tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca
    33301 cggaaacgta caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc
    33361 aagttcgcac atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt
    33421 tctgcttcta attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc
    33481 tgatcaatca gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct
    33541 ccaggatttt caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg
    33601 cttttttcgc catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag
    33661 tctccatcat tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata
    33721 tgcaggtgcc cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc
    33781 ttctttcaaa ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata
    33841 tcatttattt aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca
    33901 actggttgaa tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt
    33961 tttctctgtc tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat
    34021 catgcgtcca gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat
    34081 gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg
    34141 ttggcgttct gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac
    34201 tggttttacg gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga
    34261 gactttattc aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt
    34321 gatcaatttg ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga
    34381 tataccctct tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg
    34441 tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat
    34501 catcaaggtt ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat
    34561 cacgcttgta tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag
    34621 ttgactctca acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac
    34681 tttcttccat tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat
    34741 catcagtaaa atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt
    34801 cgagatcaca acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata
    34861 agtcaagctg acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt
    34921 ggccaacagc aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt
    34981 attattcagc catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga
    35041 aatacccaag cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt
    35101 ttttcctccg tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc
    35161 caaagttgct actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt
    35221 tttaggtagg tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat
    35281 atgactgaca cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg
    35341 ctgtgttcct ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc
    35401 tggttcattt aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag
    35461 caacccagct cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc
    35521 tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac
    35581 caaaattgga cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag
    35641 gcgttcttct aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat
    35701 ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg
    35761 aaaactatga tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat
    35821 tcttgggccg gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc
    35881 atttgtttta gcgattttga gtagttctgc agtggtttct cttacttggc tgacacttcc
    35941 tgccacactt gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc
    36001 aggggagata tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata
    36061 aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc
    36121 ttcgccggat acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa
    36181 cagcaaagtg gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac
    36241 cacacctccg cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt
    36301 tttagggacg acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg
    36361 agttctgcgg tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca
    36421 atttgggcag cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg
    36481 tacttttgat ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat
    36541 caagcttaag tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga
    36601 gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc
    36661 tcgccttttt tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg
    36721 ccttcatttg cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac
    36781 actaaaaacc gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct
    36841 cccatgtaag ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct
    36901 gatacatctt ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc
    36961 cagatacttg gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt
    37021 gtagcgcgct gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct
    37081 ctttttctca ccggttttcc t
//
LOCUS       DAJSVF010000021        36570 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSVF010000021 DAJSVF010000000
VERSION     DAJSVF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.021"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ON550_001046"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ON550_001046"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3954144.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ON550_001047"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ON550_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR3954145.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ON550_001048"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ON550_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR3954146.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ON550_001049"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ON550_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR3954147.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ON550_001050"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ON550_001050"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR3954148.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ON550_001051"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ON550_001051"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR3954149.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ON550_001052"
     CDS             complement(8054..8524)
                     /locus_tag="ON550_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR3954150.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ON550_001053"
     CDS             complement(8570..8995)
                     /locus_tag="ON550_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR3954151.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ON550_001054"
     CDS             complement(9062..9331)
                     /locus_tag="ON550_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR3954152.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ON550_001055"
     CDS             complement(9342..10937)
                     /locus_tag="ON550_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3954153.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ON550_001056"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ON550_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR3954154.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="ON550_001057"
     CDS             complement(14490..15050)
                     /locus_tag="ON550_001057"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3954155.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ON550_001058"
     CDS             15403..16377
                     /locus_tag="ON550_001058"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3954156.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ON550_001059"
     CDS             16547..17947
                     /locus_tag="ON550_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR3954157.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ON550_001060"
     CDS             complement(18025..18747)
                     /locus_tag="ON550_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3954158.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ON550_001061"
     CDS             complement(18826..19332)
                     /locus_tag="ON550_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3954159.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ON550_001062"
     CDS             19455..21254
                     /locus_tag="ON550_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR3954160.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="ON550_001063"
     CDS             complement(21322..22896)
                     /locus_tag="ON550_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3954161.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ON550_001064"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ON550_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR3954162.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ON550_001065"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ON550_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR3954163.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="ON550_001066"
     CDS             complement(24673..25311)
                     /locus_tag="ON550_001066"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR3954164.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="ON550_001067"
     CDS             complement(25312..25962)
                     /locus_tag="ON550_001067"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3954165.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ON550_001068"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ON550_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR3954166.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ON550_001069"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ON550_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR3954167.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="ON550_001070"
     CDS             complement(29105..29845)
                     /locus_tag="ON550_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR3954168.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ON550_001071"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ON550_001071"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR3954169.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="ON550_001072"
     CDS             complement(31216..32154)
                     /locus_tag="ON550_001072"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR3954170.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="ON550_001073"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="ON550_001073"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3954171.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ON550_001074"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ON550_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR3954172.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="ON550_001075"
     CDS             complement(35314..35619)
                     /locus_tag="ON550_001075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3954173.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ON550_001076"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ON550_001076"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR3954174.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6707 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DAJSVF010000022        35686 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSVF010000022 DAJSVF010000000
VERSION     DAJSVF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.022"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="ON550_001077"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="ON550_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR3954175.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="ON550_001078"
     CDS             complement(2203..4389)
                     /locus_tag="ON550_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR3954176.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ON550_001079"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ON550_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR3954177.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="ON550_001080"
     CDS             complement(4611..5051)
                     /locus_tag="ON550_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR3954178.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="ON550_001081"
     CDS             complement(5125..5664)
                     /locus_tag="ON550_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HCR3954179.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="ON550_001082"
     CDS             complement(6279..7745)
                     /locus_tag="ON550_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3954180.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="ON550_001083"
     CDS             complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="ON550_001083"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3954181.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10661..11020)
                     /locus_tag="ON550_001084"
     CDS             complement(10661..11020)
                     /locus_tag="ON550_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR3954182.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11050..11391)
                     /locus_tag="ON550_001085"
     CDS             complement(11050..11391)
                     /locus_tag="ON550_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954183.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11388..12062)
                     /locus_tag="ON550_001086"
     CDS             complement(11388..12062)
                     /locus_tag="ON550_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3954184.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13115..14413)
                     /gene="asnS"
                     /locus_tag="ON550_001087"
     CDS             complement(13115..14413)
                     /gene="asnS"
                     /locus_tag="ON550_001087"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR3954185.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14453..15643)
                     /locus_tag="ON550_001088"
     CDS             complement(14453..15643)
                     /locus_tag="ON550_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3954186.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIETIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15664..16191)
                     /locus_tag="ON550_001089"
     CDS             complement(15664..16191)
                     /locus_tag="ON550_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR3954187.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16238..19027)
                     /locus_tag="ON550_001090"
     CDS             complement(16238..19027)
                     /locus_tag="ON550_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3954188.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19174..19374)
                     /locus_tag="ON550_001091"
     CDS             complement(19174..19374)
                     /locus_tag="ON550_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3954189.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19826..20146)
                     /locus_tag="ON550_001092"
                     /pseudo
     CDS             complement(19826..20146)
                     /locus_tag="ON550_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20649..21767)
                     /locus_tag="ON550_001093"
     CDS             complement(20649..21767)
                     /locus_tag="ON550_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954190.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYYEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAIFRY"
     gene            22237..23544
                     /locus_tag="ON550_001094"
     CDS             22237..23544
                     /locus_tag="ON550_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3954191.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSESKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            23663..24862
                     /locus_tag="ON550_001095"
     CDS             23663..24862
                     /locus_tag="ON550_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3954192.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24889..26157)
                     /locus_tag="ON550_001096"
     CDS             complement(24889..26157)
                     /locus_tag="ON550_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR3954193.1"
                     /translation="MEKQLPSTVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26320..26967)
                     /locus_tag="ON550_001097"
     CDS             complement(26320..26967)
                     /locus_tag="ON550_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR3954194.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27375..27878)
                     /locus_tag="ON550_001098"
     CDS             complement(27375..27878)
                     /locus_tag="ON550_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3954195.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28001..28765)
                     /locus_tag="ON550_001099"
     CDS             complement(28001..28765)
                     /locus_tag="ON550_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR3954196.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DVEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28873..29148
                     /locus_tag="ON550_001100"
     CDS             28873..29148
                     /locus_tag="ON550_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR3954197.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29744..30694
                     /gene="yidC"
                     /locus_tag="ON550_001101"
     CDS             29744..30694
                     /gene="yidC"
                     /locus_tag="ON550_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3954198.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30873..31847)
                     /gene="lacD"
                     /locus_tag="ON550_001102"
     CDS             complement(30873..31847)
                     /gene="lacD"
                     /locus_tag="ON550_001102"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR3954199.1"
                     /translation="MKKMSIEKQECLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDESVEINVQKQIVIERIDSECEAEGMPFF
                     LEILSYDATISDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVKGYG
                     QETCYSQAEASTYFYEQSKVTEQPFIFFSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(31844..32302)
                     /locus_tag="ON550_001103"
     CDS             complement(31844..32302)
                     /locus_tag="ON550_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3954200.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKTEIATILTKEIGEDI"
     gene            complement(32299..33762)
                     /locus_tag="ON550_001104"
     CDS             complement(32299..33762)
                     /locus_tag="ON550_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3954201.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEGLEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(33744..34694)
                     /gene="pfkB"
                     /locus_tag="ON550_001105"
     CDS             complement(33744..34694)
                     /gene="pfkB"
                     /locus_tag="ON550_001105"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3954202.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMGCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            34837..35586
                     /locus_tag="ON550_001106"
     CDS             34837..35586
                     /locus_tag="ON550_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3954203.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFTDRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMREN"
BASE COUNT        10201 a         7673 c         6229 g        11583 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg
     7861 ttttttcata ttttttccat caaaaaaata agttattttc ttttatttca tttaccttat
     7921 tgaatttcaa gctattttga attttctgca aaattaaaaa cattagttat tttataatcc
     7981 tatctatttt ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt
     8041 cgtcctcttc attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac
     8101 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
     8161 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac
     8221 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
     8281 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
     8341 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
     8401 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa
     8461 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
     8521 atcgcccgtc aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc
     8581 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
     8641 cgctagataa agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc
     8701 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
     8761 acctgcaaat tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac
     8821 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat
     8881 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
     8941 ggttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
     9001 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga
     9061 gatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     9121 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     9181 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     9241 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     9301 tttttctact tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc
     9361 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     9421 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     9481 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     9541 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     9601 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     9661 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     9721 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     9781 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     9841 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     9901 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     9961 tacttttttt atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
    10021 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
    10081 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
    10141 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt
    10201 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt
    10261 ccatacagat gttttggctt attctttctt tttcttttcg atcattgcca ctgctcttat
    10321 ccaacccgca cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc
    10381 tggcaatttg tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc
    10441 aaaatgtcct tcccagatat tcgaagctcc actacgcttt caaaaatata gaaagagttc
    10501 tttaatgaaa attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa
    10561 aagtcacatg gaagaactta tacaggagaa cgcatagagt caggtgaaat acatgtatcc
    10621 cacctgactc tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga
    10681 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
    10741 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
    10801 cgatcagcca gatgatatta acaatcaatg acatgccaga ctggtgataa attacttctt
    10861 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
    10921 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
    10981 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
    11041 tgtttttgat taaaactggg caaaaaatga ttttattctt ctttttttag cttccagtat
    11101 caaatattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
    11161 ttggattagt tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc
    11221 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
    11281 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
    11341 ctgattgatt tgaggtagat cttcttttac tgcccaccgt atttccatca tttctcactt
    11401 ttctttcgct catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag
    11461 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
    11521 ttcgaaaaac cataaacaat ccctaaagca ttgaatatac catctaaagg aaccagcaat
    11581 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
    11641 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
    11701 attcccactg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca
    11761 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
    11821 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
    11881 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga
    11941 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
    12001 tctgctcggt ctcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc
    12061 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
    12121 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc
    12181 aaacaattga atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt
    12241 gccggattcc ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acctaagctt
    12301 ccgtcaatca gaccactgat gacgccatca gaacatatat ttataggctc atctaagact
    12361 tctagtaaat gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa
    12421 ctttatctgg tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct
    12481 gaaaatacgg acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat
    12541 gctgctttat ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct
    12601 ttgtaaatgt ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt
    12661 cttttgcttt cttctcttgt ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa
    12721 ctgcgagagt tcgcagttct tttttagaat aaacggtgct ctccggccaa ctcctcggtc
    12781 acaagtcaca atagtcatca aacatgaaaa actgtttttt gaaattactc cttgtgactc
    12841 ggagctgaac ggcaggcgca caacctcttc cttaaacggt gttcaaaagc cagtttctcg
    12901 gtattaagcc aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt tttcttaata
    12961 ctcgaaacta aacagctttt tcacaacctc tccactaaac ggtattcaaa agcttacatc
    13021 tgcggtaata agtcccgcca attcaaaaac atgaacagct gtttttgctt ggctgttcta
    13081 attacttgat gcagaacagc tttttcatca cctcttatgg ataaattcgg ttcaacaaac
    13141 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
    13201 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
    13261 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
    13321 ctgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
    13381 cacagatcac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
    13441 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
    13501 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
    13561 tcgcatcatc ataagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
    13621 tgtctcgtcc tagtacatct aatgcatatt cgcaatgatc taaaacattt tgtactaaga
    13681 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
    13741 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
    13801 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
    13861 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
    13921 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
    13981 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
    14041 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
    14101 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
    14161 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
    14221 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
    14281 ggaaaaaggc tgttccgtct cttaattgta aaaatgcgat ttttccgctt gatcgcttat
    14341 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
    14401 gaatttgttc cacgtatgac tctcctttta tattccttga atttctttag gattaatttt
    14461 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
    14521 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
    14581 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
    14641 tgcgtagcgt ttcttttaca ttaggaaaaa gataaaaagc tccttgtggc ttttcaagag
    14701 aaacacccgg cagttctgcc acaagcgaat ataacgtatt caggcgttct tcaaaggcgt
    14761 tccgcatttc ttccactgta tcttgctcgc ctttcaatgc ttcaacagct gcatattgac
    14821 tgactgctgt cggattactg gttgactggg aagcaacagc gatcattcca ttgatgatca
    14881 caggatttcc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
    14941 cgttgatgat gatagtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
    15001 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
    15061 ttactgccca ttcaccgatt gcctgcagtt cattcttgct ataaatcatt ccagtcggat
    15121 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
    15181 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
    15241 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
    15301 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
    15361 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
    15421 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
    15481 tttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
    15541 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
    15601 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
    15661 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
    15721 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
    15781 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
    15841 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
    15901 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
    15961 ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt tctttgcgat
    16021 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
    16081 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
    16141 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
    16201 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
    16261 ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg atcggtactt
    16321 ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt cttcgatcca
    16381 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
    16441 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
    16501 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
    16561 ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc
    16621 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccac
    16681 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
    16741 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
    16801 cgatcagacc tgtaagtgct gtctctaaat aatcgatata tgcttgattg ctgacttgat
    16861 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
    16921 aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga tagtgtcgat
    16981 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
    17041 cagtcgacgg caacatagcc gcttgaaaat catggagatg aaaaatcatc tgctgcgctg
    17101 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
    17161 tcagcaagta ttgcttgtct aattgatcaa ataggctata taaacttgct aaatcgatcc
    17221 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
    17281 gctgctggat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
    17341 gcagctgatc caacatctct tttgtcatca tgacttcttc tggatactgt ccgttaggtt
    17401 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
    17461 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
    17521 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
    17581 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
    17641 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
    17701 gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
    17761 aacgaacaaa gtcttgttcg taaaatgact gatttcttgc taaaaatcca atacctctgt
    17821 gcgcgatatc tgcaaagaat gaatggttca aatttgttaa atgtaattca tccaaatcac
    17881 cagtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
    17941 gttctggcgg ttccacaagt gtgcctttaa aacgctgcaa atcaatatag tggcgatagc
    18001 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatgtctt
    18061 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag
    18121 gtgtagccaa ataactcaat ggcagcagat aaccgattgt tttacccatt cctgtagctg
    18181 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagacc gcattcatca
    18241 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa cattttttct tttgcttgtt
    18301 tttttcttgg atatgtccgc tcgccataat ggacggatgt gaatagttcc acttcttttt
    18361 tctgcaaagc tagcccatcg acgatttcta atgagggatc aagtggctct ggtttttctt
    18421 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    18481 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    18541 aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    18601 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    18661 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    18721 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    18781 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    18841 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    18901 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    18961 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    19021 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    19081 tgcaaaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    19141 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    19201 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    19261 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    19321 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    19381 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    19441 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    19501 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    19561 gattacataa agtgaagaac cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    19621 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    19681 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    19741 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
    19801 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    19861 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    19921 tttcttaatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    19981 cttctgtttc aggaatcgca gaatcaagat ttggtgtctg tacataaaca tttttgtaca
    20041 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    20101 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acttccttat
    20161 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    20221 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    20281 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    20341 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    20401 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
    20461 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
    20521 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggcaaa
    20581 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
    20641 aacctctttt aataacgaaa aatcgctgtc agactctttt catctggcgc atctttttga
    20701 tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc attcaacact
    20761 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
    20821 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
    20881 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
    20941 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
    21001 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
    21061 ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa agaatccttc
    21121 aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
    21181 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
    21241 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
    21301 tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt catggagtcg
    21361 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
    21421 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
    21481 taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
    21541 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcgtaatat
    21601 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
    21661 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
    21721 ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa cgtacccctc
    21781 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
    21841 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
    21901 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
    21961 gctgataaac taagaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
    22021 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
    22081 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta accttccata acgtgcgttc
    22141 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
    22201 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
    22261 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
    22321 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
    22381 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
    22441 atcatcggtc tttacatcgt tcttgatcaa gtaaaagaag gaaaactatc atgggatgat
    22501 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
    22561 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
    22621 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga atctaaattc
    22681 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac gatcgttaat
    22741 gcatcaggac tgaataactc ttatcttggc gaaaaccgac cagaaggtac tggagaaaac
    22801 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
    22861 tttccagaaa tactggacgt atctagcact acgacacaaa tgtttggcga aaacacgcaa
    22921 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
    22981 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
    23041 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
    23101 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
    23161 tcagaaaaag agctcggaaa agctaacgca agtattccta gcctaaaaac cattgatgta
    23221 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
    23281 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
    23341 actgacaacg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
    23401 accttggcag acgataaatt aggctattta gaagacaaca atgcgccatc aacagagatc
    23461 attacggata aaacagtgga aaaagcgaat atgttcgtga ttggctggcg acgcgtcgca
    23521 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa gcatctttcc atcggaaaga
    23581 tgtttttttg cttttactag tttaaataac agcttttttc atctattttc gttatactgt
    23641 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
    23701 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
    23761 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
    23821 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
    23881 ttatttcaca gaaaaacgaa ggctgctgaa tggtctttta ttcaatgttt tcctcatcag
    23941 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatattttcc ttggcggact
    24001 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
    24061 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
    24121 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
    24181 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
    24241 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
    24301 gcctcgatac aatcaagact tcatcgttgt gttaggcgct gggttgatca atggagaaac
    24361 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
    24421 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
    24481 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaattc ccgaaagaga
    24541 cattcttgtc gaaaccaatt cgacaaccac tttggagaac atgctctatt caaaggaaat
    24601 catggatcag caaatgaaag ggccataccg cgcgatcttc agttccaaca attaccacat
    24661 ttttagagcc ggtctttatg cgcgacaagc caaattgaaa gcaaatggta ttggtgcaaa
    24721 aacagctttc tactacttgc ctaatgcgtt tttgagagaa tacatcgcca tcttgatgat
    24781 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
    24841 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
    24901 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
    24961 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
    25021 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
    25081 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
    25141 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
    25201 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
    25261 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
    25321 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
    25381 atttcgccaa acaggatgag gaatccattg tgcataccgt gcttttaaaa agacgatcga
    25441 acggctgaat acccagccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
    25501 cagcaacata ctccattcag ggatcacacc catttggtaa tgtaggtgag aaacaccata
    25561 gctttctgtc ataaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
    25621 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
    25681 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgcttt
    25741 atccaatcgg aacaaacgtg ctgtattatc tgctaacgct ccccccattt ggactgctgt
    25801 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
    25861 tggaataagt cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
    25921 gtttccacga ctagaaagac cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
    25981 aactggtagt agataaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
    26041 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
    26101 cagccacttc agcatgtaca ctaaaatgcg tactgtactc ggtaattgtt tttccataaa
    26161 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
    26221 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
    26281 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
    26341 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
    26401 atgacaaata taatttccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
    26461 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
    26521 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
    26581 tttctgcgta cctggatcat tccagccgat gtaagtatgt tcttgcaagt cgatttcatt
    26641 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
    26701 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
    26761 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgatttctg gcagttcgtg
    26821 atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta ccgtttttgg
    26881 atcattgaca gatgagtacg catttaataa ctgttctact tctcttttta taaaaaaata
    26941 ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa ccaaagtgca
    27001 tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat cccggactct
    27061 tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat tattctatgt
    27121 acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc aactgcaaac
    27181 agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc
    27241 aaaagcttgc atctgcggta ataagccgaa acttcacaaa aatttgagaa gtaattttcg
    27301 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat atggtctatt
    27361 attggtttta cctgttaaat cgaggattct ttcccaaagt aacgttgcca tctcttacgc
    27421 atacttgcag acatgggctg agccgcttct ttgactgcgc agtatttgtc cacgaatgtc
    27481 acaatatatc cttctttata cttaggcggt gcgatcgttg caccccacat gtgcttcaaa
    27541 atgatatctc gctcaagatc cgacaaatcg gtgatttttt ctgcattctt tacagcaatt
    27601 cttggatgga tatacgcatg tgtcccctcg tcaaatttcg tcgtacgcca atcatagtag
    27661 aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc ccattttttt
    27721 gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt ggaatgaaca
    27781 tgctgtgtgt aatttgctaa ttttttcact gcttctgttt ctaaaagatc ttgtacataa
    27841 gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgatct gccacctttt
    27901 tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg attattctag
    27961 tcgctctaga taaaatagct aaagcttaat agttccttaa tcataaagaa aaatgataaa
    28021 gaacgatccc tgctgcgact ccgacattca atgattccgc atttcctttc attgggatat
    28081 atacgttctg atcagtcaaa gacaagattt cctgacttac tccctgacct tcgtttccca
    28141 tgattatagc gaaatcctct gttttttcta ctttgtctaa tgcatgtgct tcttcattta
    28201 attctgtacc gtacaccgta aaccctttgc ctttcattac agggatcact tctgccaaat
    28261 ctttttgtaa cacaggcaaa tgataattgc ttccctgcat gcttcgcagc acctttgtac
    28321 tataaatatc tgccgttcct ttacctaata aaacaccagc cataccaaac gcatctgccg
    28381 tacggatcat cgttcccaca tttccaggat cctgtacatt atctagtaaa agccagcctc
    28441 ctgaaaaatc tggcgtatct ttagatgtta acgtcatcac tgctaagatc ccttgcggtg
    28501 taggtagatc tgacatagcg gtcatgacct catctgaaac tagaaaagta gaaatctctt
    28561 tgtttacagc cagccagtct ccccattctt tcatacctct ctgtgtcaca aaaagttctt
    28621 ttatttctac atcagacgac gcagcttctt cgatcagatg gaacccttcc aaaagatatt
    28681 ctgccttttc ttctctgtat ttctttttat gtagtttttt taattctttg attcttggat
    28741 tttttgctga ttggatctct ttcacattcc ttcacctcac ctgattatat catggaatag
    28801 gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt gatttataaa acaggaaaga
    28861 aaggtgacga acatgcgcaa agttaaaatg aatgtccaag gacgtgtcca gggcgtggga
    28921 tttcgctata tgaccaagat ggttgctgat cagttaggcg tgaccggaac ggtaaaaaac
    28981 gaagacgacg gctcagtcac aatcgaagca atcggaaatg acgacatcat ccaaaaattt
    29041 atcgaagaag tcaagaagtc tccgagccca agtggacgag tgcagtatgt ggatgtccaa
    29101 gaggatccga tgattgagga acgaaagaaa tttgatgtgg tgggataagg ttgtgaaaag
    29161 gtcgctgagt tttgagtatt aaggaaaatt ttgtgaaaat agctttccac atttttgcat
    29221 aaatttgact taataccgag aaactgactt ttgaacaccg tcgatcaggt tgcgaaaaag
    29281 ctgatctgca tcaagcatta aggactggtt gtgaaaaagt cgcttagttt tgagtattaa
    29341 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ataccaagaa
    29401 attgactttt gagcaccgtc gatcaggttg tttttgaggc gctttgcctc aagtattaaa
    29461 gataggtagt aaaaaagctg atctataact tgcaataaat ttgcttaacc catattttcc
    29521 agacagaaca aaaaagtttt tagtttttta aataagaaag aaacacttgg aatcactctt
    29581 ttctttccaa gtatttcttt cttgattttt tgtctttttt caaaattttt cgctacgaaa
    29641 aagaaagaat atttatatga aatttctatg aagttgattg aattccatgc gcttttttag
    29701 tatagttaag tttgtgtaaa aattatagaa gaggaagtat tctatgaata aatggaaaaa
    29761 ctggttattg ggatccggac ttgtcagtat cctgctcttt ttatctggct gtgtgcggat
    29821 ggattcaaac ggaaaccctg acacttcagg aatcgtttat cgagtactcg tccatcctat
    29881 gggccaagcg attacatatc tagttgagaa tttcaattgg tcttacggct gggcagttat
    29941 tgcaatgact gtgatcgttc gtatcatcat cttgccacta ggtatcagtc aatcgaaaaa
    30001 aacaatgatc caatctgaaa aaatgcaagc gttaaaacca caagttgaag cagcacagca
    30061 aaagctaaaa gctgcaacaa cacgtgaaga acaaatggca gcacaagcag aaatgcagca
    30121 agtatatcgc gaaaacggat tgagtatgac cggcggaatc ggctgtctgc ctctattgat
    30181 ccaaatgccg atcttctctg ccttgtattt tacggctcgc tacacagaag gtatccgtga
    30241 atcatctttt tacggcatcg atctaggctc accaagtatg gtgcttgtgg ccatcgcagg
    30301 ggtcgcttat ctgcttcaag gatatatttc aacaatcggt attcctgaag aacagaaaaa
    30361 gacgatgcgt acgatgctga tcgtctctcc tgctatgatc gtcttcatgt cgatcagtgc
    30421 gccagccggt gttacacttt actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt
    30481 catcacaaac gttatgatga aaccacgttt gaaagcacaa gtggcagaag agttgaaaca
    30541 aaacccgcca aaacaagtcg tcactccaag aaaagacgtg acagaatcag caaagccagc
    30601 tgacaacaaa caagcaacaa aacaaatcaa acaaccaaaa aacagtacaa ataacaaagg
    30661 ccgcaacgca ggaaagcagc agaagcggaa gtagatagta aataagagtg taataaaaat
    30721 tcattaacag ttttagtaat taaaaatatc gaccaagatt tttgaaaaag aaatctggtc
    30781 gatattttta tttaagtgaa tgtttaattt gaatcatcga ttgatcgcta tctaattgca
    30841 gcagcatctt tttatgaaga aacaactttt tcttatctat caacgtagac aggtgttgct
    30901 gtttcttcta atagtgtact taattcttta atattttgtt ttccttcttc taacatccaa
    30961 ctactcgctt tttcttcgcc ttcggtaaca aaaggtgcta ctcccttcgc ccatgttgct
    31021 cgtccacata agacaccatt gaatgtcgaa cctgattgtt ttgcaaaacg caatgtatct
    31081 tgaaatagtg cagcgctgac tcccgcacta aaaaaaataa acggttgttc agttactttc
    31141 gattgttcat agaaataagt ggatgcctct gcttgactat aacaagtctc ttgcccatat
    31201 cctttcacaa atctcatatt cactggtact tctactttca atacatccac ttgataacgt
    31261 tcttttgaaa attctcgcat ggcttcaatc actttatgcg gttttacttt tgcataggca
    31321 agagaagacg gatcgctaat agttgcatca taagaaagga tctctaaaaa gaaaggcata
    31381 ccttctgctt cgcattctga atcaatccgt tctataacta tttgtttttg cacattaatc
    31441 tctactgatt catcaacgtc atagtacaat aaaaatttac aagcatccgc acccacttct
    31501 tttaaccttt tcactgacca actattcagt aaatctggta gtcggcccgg cattttcgca
    31561 tcatagccgg ttttttcata agctagcaac aagccacatt ctttctctcg aagcgctgct
    31621 gcaggcagac catattctgg atcgagtaaa atagcggaag catacggagt caggtactga
    31681 gaaaccatct gtttaaattg gatgatatct tctgatgtag cttctttata ttggttgatc
    31741 atttttttca aagcaccacg ctggtcgatt gctaacgcgc taataattcc agcttcatcc
    31801 gtcaatcgct tcaaacattc ctgtttttct atactcattt tcttcatata tcttcaccta
    31861 tttctttagt taatattgtg gctatttctg tttttgattt tgctgctctc aactgttctg
    31921 taacctcttt tcgcatcaat gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc
    31981 ctgcttctga atctgggata aataacgcaa aaataaaatc aactgactgt ctatctaaac
    32041 tgttccactc aatccctttg ttcagtgtga cgataatcaa actaggttcg ttgattgttt
    32101 gagacttcgc atgagggata gcaaaacctg acattattcc tgtagtacct tctttttctc
    32161 tttctagaag ttttcgatat acttcttttt cgtcatgagc gattcctaaa cagaatgttt
    32221 gctttgctaa aaacttaaat gcttcttctt gactatctaa tggctgttgt aaaaaaatgt
    32281 tctcttcatg aatgattgtc atgataagcc tcctgctctt ccttcttcga taactgtacg
    32341 atttgctgct ttattatcca tcttcttgtc tttatcagtt actcttttct tttggatcaa
    32401 acctagtatc actccaccaa cgatcgaacc aataagaatc gctgtaaccc attggatttt
    32461 tcccgtcact acaggtaaca ctaaaaaacc gccatgtggg gctggtactt ggacaccaaa
    32521 cagataggtc aagacagcag caattgatga gcctaacatc atcgttggta atactcggat
    32581 cgggtctttc gcagcaaacg gaatagctcc ctccgtaata tgagttgatc ctaaaataaa
    32641 attaaccaaa cctgcattac gatcattagt atcaaaatat ttaccaaaca ataaagttgc
    32701 aaaaccagta atcaacggtg gtgcaatgca agctactgac acgccagcca taaaagtcgt
    32761 gtttccttgt gccaaaagag ctgtacctgt tacatatgcc gccttattaa tcggtcctcc
    32821 catatcaaac gcacacatgc aaccaacaat cacgccaaga acaagtggat tcgaatgttc
    32881 aaatccagct aaaaagctca tcattccttc atttatagcc ttcattggac ccgccagcaa
    32941 agacatcacg atgccgataa acactacacc aataactgga tataagaaga ttgccttcaa
    33001 gccatctagt tgtttaggta aaattgtcag tagcttttgc aaaaataaga taaaataacc
    33061 tgctaagaac ccagctaaaa tacctcctaa aaatccagta ccgccactca tggccacgat
    33121 tccaccaaca aatccaacga ctaaccctgg acgtttcgcg atcccttctg caatataagc
    33181 tgctaaaact ggaaccatca tccccattgc tgcatcgcca accgttttta acatcgccgc
    33241 tgtagcattg tattgtgcat ggtctggatc aaaagaatag attccccaaa cggcaaatga
    33301 aatagcaatc aatactccgc cagctaccac gaaaggcaac atatgagaga cgccattcat
    33361 taaattttta tagatgctgc gaccaaaatt agcaccactt atcgtttctg attctaaagt
    33421 atcaacagct tcattatctg ctaagatggt cccttttcct gccaatgctt cgtcaatcag
    33481 ccgttgtgca tccttgattc ctgtactgac agaaacatca atgactcgtt ttcccgcaaa
    33541 tctttctgaa tggacatctt tgtctgcggc aataatgaca gcatctgcct gtttgatttc
    33601 ttcagccgtt aattcattct cgatcccaat ttgtccatgg gtctccactt tgatcgtgat
    33661 gccttttttt ctagctgctt gctctaatcc ctcttgcgcc atgtaggtgt gtgcaattcc
    33721 tgtcggacaa ccggttgctg caattagttg atattttccc atctttcgaa cctctcttcc
    33781 ttgattttga tttgtttact taattcctcc acatccaaaa aatcagtcag tcctttacga
    33841 aaagcagtag agctgcctgc tgctatgctt ttacgtaacg tttcatcaag cggtcgtcga
    33901 ttcatgtatc ctactaaaaa agtgcctaac atggcatcac ctgcacatgc tgtattgaca
    33961 acttttcctt ttggcgagtt tcctcgaatc accctgtctt tcgtaaaaag aatcgctcct
    34021 ttctcaccaa gggataacaa aacattttct gcacctgact ctaccagtcg ttgtccataa
    34081 acaaagtaat cttcatctgt aaccattttt cgtccgaacc aagaagaaag ttcttcctca
    34141 ttcggtttca gcaaaaatgg tcgatagggc aaacaaccca tgacctcttg atcactgcta
    34201 tcaataatca aatgaatctc cttttcctga caaatccgac tgatttccac taaaaccttc
    34261 ggagacattc ctcttggtaa actcccagaa acacaaagat aatcctctgc ttgtaatgct
    34321 cttatttgat ttaaaaaatt atggacagct gcctgcggta tttcaggtcc ttgattcact
    34381 aatttgtatt cttctctcgt ttgattgact tgtgtaaata cattgatacg cgtcatacca
    34441 ggcacttcaa taaactctgt ttggatttgt ttcttctgta aatagtcttc tatatatttt
    34501 cctgtgaaac cagcagaaaa acctaatgcc gtacttggga aatctagcat ctttaatact
    34561 agtgacacat ttaccccttt tccgtttgcc tgaatgtcat cctcctttgt acggtttacc
    34621 ataaaaggat gcatctcctc tgtttcaata aatagatcaa ttgccaaatt catcgtgcat
    34681 gtgtagatag acataaccaa tgacctcctt gaatctatta tgaaatacgc gaaagagaaa
    34741 atgttttctt aatcgttcca acacaaagaa aacctttaca tttataaaca catcgtgcta
    34801 ttttttaact aacttttagt gaaagaagga aagaatatgg atcacaaatt aagtgaaaca
    34861 gaacagtacc tctggaactt tatcgaacac cacatcctag agatccctaa ctactctatc
    34921 gtgaaactaa gtgaacaagc aaatgtctca acagccacca ttgtgcggac aatgaagaaa
    34981 aaaggctatg aaggatttac ttcgtttaag caccatctaa aagaaggaga aaacaagaac
    35041 atcaattttt ctttcttaga taaagtagac aaaggtatcc gtcgagccat tctaaaaaat
    35101 gaacaagaag tggtacgtac gatcaatatg ttggaaatcg ggaacattga agatgcaata
    35161 caaagaatca aatttactga ccgggttttt gtctttgccc gaggattttc agaaatgatt
    35221 ggtcaagaga tgctcgtaaa acttcaatta acaggaaaaa attgtcaaat gcatactgat
    35281 ccagagatta tcaaaagcat cagtcggaaa ctaactaaga aagacagcgt tatttttgtt
    35341 tctttgaacg gcgagacaaa agaactcgtc accgccgcaa agaactgcta tgatgcagag
    35401 attggttcga ttctagttac agccaatcat tcctctacac tcaaagatta ttgcgagata
    35461 aatctcgtag gtttcaaatc agaaggttct tatttccctg attatgaagt acgttcacgc
    35521 ttgcctctcc agatcatcgc gagaatcctg cttgacgcct atgtattgcg tatgagggag
    35581 aattgagaag cagtttatta tggttggtta cagtttaaaa catcacattt tttattcttt
    35641 atattgtatg gactcacgta aactttttat gtataaagtt attcac
//
LOCUS       DAJSVF010000023        34510 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSVF010000023 DAJSVF010000000
VERSION     DAJSVF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.023"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            212..604
                     /locus_tag="ON550_001107"
     CDS             212..604
                     /locus_tag="ON550_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3954204.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ON550_001108"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ON550_001108"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR3954205.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="ON550_001109"
     CDS             3165..4775
                     /locus_tag="ON550_001109"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR3954206.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="ON550_001110"
     CDS             5067..5936
                     /locus_tag="ON550_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR3954207.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ON550_001111"
     CDS             6101..7372
                     /locus_tag="ON550_001111"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3954208.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ON550_001112"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ON550_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR3954209.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ON550_001113"
     CDS             8763..9026
                     /locus_tag="ON550_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR3954210.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ON550_001114"
     CDS             9471..9920
                     /locus_tag="ON550_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3954211.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ON550_001115"
     CDS             9977..11395
                     /locus_tag="ON550_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3954212.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ON550_001116"
     CDS             11395..12255
                     /locus_tag="ON550_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR3954213.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ON550_001117"
     CDS             12429..13202
                     /locus_tag="ON550_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3954214.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ON550_001118"
     CDS             13349..13714
                     /locus_tag="ON550_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR3954215.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ON550_001119"
     CDS             complement(13862..15070)
                     /locus_tag="ON550_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3954216.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ON550_001120"
     CDS             complement(15089..15367)
                     /locus_tag="ON550_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954217.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="ON550_001121"
     CDS             15521..16216
                     /locus_tag="ON550_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3954218.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="ON550_001122"
     CDS             complement(16658..17455)
                     /locus_tag="ON550_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR3954219.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ON550_001123"
     CDS             complement(17452..18636)
                     /locus_tag="ON550_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3954220.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ON550_001124"
     CDS             complement(18661..19908)
                     /locus_tag="ON550_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3954221.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ON550_001125"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ON550_001125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR3954222.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ON550_001126"
     CDS             21888..22202
                     /locus_tag="ON550_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR3954223.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ON550_001127"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ON550_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR3954224.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="ON550_001128"
     CDS             22887..23531
                     /locus_tag="ON550_001128"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR3954225.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ON550_001129"
     CDS             23546..23875
                     /locus_tag="ON550_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR3954226.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ON550_001130"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ON550_001130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR3954227.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ON550_001131"
     CDS             24863..25687
                     /locus_tag="ON550_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR3954228.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ON550_001132"
     CDS             25680..26027
                     /locus_tag="ON550_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR3954229.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ON550_001133"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ON550_001133"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR3954230.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ON550_001134"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ON550_001134"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3954231.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON550_001135"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON550_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR3954232.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON550_001136"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON550_001136"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR3954233.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="ON550_001137"
     CDS             31657..32451
                     /locus_tag="ON550_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954234.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ON550_001138"
     CDS             32441..34483
                     /locus_tag="ON550_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954235.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11094 a         6120 c         7248 g        10048 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat
//
LOCUS       DAJSVF010000024        33593 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSVF010000024 DAJSVF010000000
VERSION     DAJSVF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.024"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            281..2752
                     /locus_tag="ON550_001139"
     CDS             281..2752
                     /locus_tag="ON550_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3954236.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2855..4591)
                     /locus_tag="ON550_001140"
     CDS             complement(2855..4591)
                     /locus_tag="ON550_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR3954237.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4588..5292)
                     /locus_tag="ON550_001141"
     CDS             complement(4588..5292)
                     /locus_tag="ON550_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954238.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5423..5926
                     /locus_tag="ON550_001142"
     CDS             5423..5926
                     /locus_tag="ON550_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR3954239.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5886..6224
                     /locus_tag="ON550_001143"
     CDS             5886..6224
                     /locus_tag="ON550_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR3954240.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6245..7663
                     /gene="ffh"
                     /locus_tag="ON550_001144"
     CDS             6245..7663
                     /gene="ffh"
                     /locus_tag="ON550_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR3954241.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7775..8353
                     /locus_tag="ON550_001145"
     CDS             7775..8353
                     /locus_tag="ON550_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR3954242.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8357..8878
                     /locus_tag="ON550_001146"
     CDS             8357..8878
                     /locus_tag="ON550_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3954243.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8957..9511)
                     /locus_tag="ON550_001147"
     CDS             complement(8957..9511)
                     /locus_tag="ON550_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3954244.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9764..10039
                     /gene="rpsP"
                     /locus_tag="ON550_001148"
     CDS             9764..10039
                     /gene="rpsP"
                     /locus_tag="ON550_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR3954245.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10051..10302
                     /locus_tag="ON550_001149"
     CDS             10051..10302
                     /locus_tag="ON550_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR3954246.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10427..11218
                     /locus_tag="ON550_001150"
     CDS             10427..11218
                     /locus_tag="ON550_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954247.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11388..11909
                     /gene="rimM"
                     /locus_tag="ON550_001151"
     CDS             11388..11909
                     /gene="rimM"
                     /locus_tag="ON550_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR3954248.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11899..12660
                     /gene="trmD"
                     /locus_tag="ON550_001152"
     CDS             11899..12660
                     /gene="trmD"
                     /locus_tag="ON550_001152"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR3954249.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12796..13143
                     /gene="rplS"
                     /locus_tag="ON550_001153"
     CDS             12796..13143
                     /gene="rplS"
                     /locus_tag="ON550_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR3954250.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13516..14178
                     /locus_tag="ON550_001154"
     CDS             13516..14178
                     /locus_tag="ON550_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954251.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14257..17484
                     /gene="fss3"
                     /locus_tag="ON550_001155"
     CDS             14257..17484
                     /gene="fss3"
                     /locus_tag="ON550_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3954252.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17598..17912
                     /locus_tag="ON550_001156"
     CDS             17598..17912
                     /locus_tag="ON550_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3954253.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17925..18299
                     /locus_tag="ON550_001157"
     CDS             17925..18299
                     /locus_tag="ON550_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3954254.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18309..18653
                     /locus_tag="ON550_001158"
     CDS             18309..18653
                     /locus_tag="ON550_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR3954255.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18724..20076
                     /locus_tag="ON550_001159"
     CDS             18724..20076
                     /locus_tag="ON550_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3954256.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20136..21278
                     /locus_tag="ON550_001160"
     CDS             20136..21278
                     /locus_tag="ON550_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR3954257.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21303..21557
                     /locus_tag="ON550_001161"
     CDS             21303..21557
                     /locus_tag="ON550_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954258.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21813..22997
                     /locus_tag="ON550_001162"
     CDS             21813..22997
                     /locus_tag="ON550_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3954259.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22994..23131
                     /locus_tag="ON550_001163"
     CDS             22994..23131
                     /locus_tag="ON550_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3954260.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            23136..23357
                     /locus_tag="ON550_001164"
     CDS             23136..23357
                     /locus_tag="ON550_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954261.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            23370..23870
                     /locus_tag="ON550_001165"
     CDS             23370..23870
                     /locus_tag="ON550_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3954262.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            23967..25814
                     /locus_tag="ON550_001166"
     CDS             23967..25814
                     /locus_tag="ON550_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR3954263.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            25817..26713
                     /locus_tag="ON550_001167"
     CDS             25817..26713
                     /locus_tag="ON550_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3954264.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            26763..27152
                     /locus_tag="ON550_001168"
     CDS             26763..27152
                     /locus_tag="ON550_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3954265.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            27139..28641
                     /locus_tag="ON550_001169"
                     /pseudo
     CDS             27139..28641
                     /locus_tag="ON550_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            29386..31212
                     /gene="ltrA"
                     /locus_tag="ON550_001170"
     CDS             29386..31212
                     /gene="ltrA"
                     /locus_tag="ON550_001170"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3954266.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            31346..32164
                     /locus_tag="ON550_001171"
     CDS             31346..32164
                     /locus_tag="ON550_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3954267.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            32257..33444
                     /locus_tag="ON550_001172"
     CDS             32257..33444
                     /locus_tag="ON550_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR3954268.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT        11239 a         5772 c         7236 g         9346 t
ORIGIN      
        1 ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat tttaccaaaa
       61 gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt
      121 tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt
      181 gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt
      241 ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa
      301 tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc
      361 atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc
      421 aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt
      481 tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta
      541 taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga
      601 aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt
      661 catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt
      721 tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca
      781 agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc
      841 attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg
      901 agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca
      961 gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc
     1021 tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag
     1081 tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac
     1141 gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa
     1201 tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac
     1261 gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat
     1321 cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga
     1381 caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca
     1441 ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt
     1501 gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga
     1561 gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat
     1621 tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa
     1681 tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat
     1741 caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat
     1801 ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc
     1861 tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca
     1921 attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc
     1981 catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc
     2041 ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg
     2101 acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt
     2161 ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc
     2221 acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga
     2281 agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc
     2341 aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa
     2401 tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct
     2461 cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg
     2521 tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat
     2581 ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag
     2641 gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc
     2701 gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt
     2761 atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca
     2821 tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga
     2881 aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt
     2941 agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt
     3001 ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa
     3061 agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt
     3121 cgctttttga atattggaca gcattttcaa tcagattttt gataattgga taaaggatat
     3181 ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt
     3241 cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct
     3301 ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat
     3361 ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat
     3421 cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta
     3481 attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg
     3541 ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca
     3601 aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat
     3661 gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt
     3721 accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca
     3781 gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta
     3841 acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc
     3901 tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt
     3961 ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct
     4021 ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa
     4081 agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg cttgtcggtt
     4141 ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata
     4201 aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca
     4261 atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt
     4321 caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga
     4381 attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga
     4441 tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa
     4501 aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga
     4561 agagacgttc ataaattttt ttccatttca tcgttttggt tcctgaaact tatagccaaa
     4621 tcctctaata gtcaccaagt actttggatg ttttggatct cgctcgattt tttctcgcaa
     4681 atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg
     4741 gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa
     4801 ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt
     4861 caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg
     4921 cttacgaggt tcaatccgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact
     4981 aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg
     5041 gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg
     5101 agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga
     5161 ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc
     5221 ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag
     5281 tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga
     5341 atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct
     5401 gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg
     5461 tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga
     5521 agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga
     5581 aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag
     5641 cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca
     5701 ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc
     5761 gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta
     5821 ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga
     5881 gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct
     5941 tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag
     6001 gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga
     6061 caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc
     6121 gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa
     6181 aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga
     6241 aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa
     6301 aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt
     6361 actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg
     6421 tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt
     6481 tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa
     6541 aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg
     6601 taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga
     6661 cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc
     6721 tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt
     6781 agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga
     6841 tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt
     6901 gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca
     6961 gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc
     7021 agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa
     7081 attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg
     7141 ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga
     7201 acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga
     7261 tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca
     7321 agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat
     7381 ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg
     7441 tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca
     7501 attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg
     7561 tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg
     7621 aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt
     7681 cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac
     7741 taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac
     7801 agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa
     7861 gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt
     7921 aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa
     7981 ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag
     8041 aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg
     8101 cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca
     8161 ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa
     8221 gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg
     8281 gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag
     8341 aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta
     8401 tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac
     8461 tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca
     8521 ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt
     8581 ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc
     8641 ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg
     8701 attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc
     8761 tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga
     8821 aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa
     8881 gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt
     8941 attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa
     9001 tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg
     9061 caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg
     9121 attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga
     9181 ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag
     9241 catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt
     9301 ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt
     9361 cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt
     9421 caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac
     9481 taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata
     9541 ataatcttac aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca
     9601 tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa
     9661 aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt
     9721 acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg
     9781 tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg
     9841 ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc
     9901 tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc
     9961 ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc
    10021 taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca
    10081 atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa
    10141 gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa
    10201 caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg
    10261 aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg
    10321 aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa
    10381 acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat
    10441 gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga
    10501 caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac
    10561 caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc
    10621 tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc
    10681 ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta
    10741 cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc
    10801 aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc
    10861 atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca
    10921 gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat
    10981 ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta
    11041 tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg
    11101 tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat
    11161 cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa
    11221 tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag
    11281 cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct
    11341 tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact
    11401 tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa
    11461 caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg
    11521 gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat
    11581 tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa
    11641 aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac catgagatca
    11701 tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac
    11761 caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat
    11821 atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc
    11881 cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa
    11941 ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat
    12001 atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac
    12061 ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt
    12121 gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga
    12181 ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt
    12241 cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga
    12301 gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg
    12361 cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt
    12421 ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa
    12481 gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga
    12541 aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg
    12601 aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga
    12661 ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc
    12721 aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat
    12781 aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct
    12841 gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt
    12901 actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc
    12961 agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca
    13021 ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca
    13081 aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga aatcagaaga
    13141 taaacacgga aaacttcctt ggaaatctga aatttgtgaa aagtccttgt atatcaaggg
    13201 ctttttcttt tttctctatc acagttttaa gccatttttg gctgttttgg accatttttc
    13261 aatcatattc ccttaaaaat tgtcctagat tgtccgacat tgactatcag tgtcgtcaaa
    13321 atagggcaaa atttagtagt cagcttgacg acaaatataa tacgaaaaat accaaaacat
    13381 ttaccgaatt tagcaacttt tgaacccaag tttctcaaag ggtcagctat tagcaaaaaa
    13441 gacaaaacat gtacttttct actaaaaagt tgatgtgata ggcttctatc taaataacta
    13501 ttaggagttt ttatcgtgaa gcaaaatgat ggacgtatca tttggaaaaa tcaaagtgag
    13561 ttaaaactga ttttaacaat taatgaattc atcgaaaaac acggaattac atctagccgt
    13621 caataccaga aaaagctatc agaaaatcca aattctgcac caagtatgtg gttcattaac
    13681 aaaaaatatg gatcatggga gaatttattg attagtatag gacgagaaaa tactgggtat
    13741 ggaaaatggg ctagaatgtc agaacaagaa ttattggaaa tagtagaagc ctttattaag
    13801 tgcgagaaga taacctctca acgaatgtac gaacaaaaat cagtagggaa gaatattcct
    13861 tcacttagta ccattaaaaa gatgttagga gatatacgtc cactttttaa agaaaaaaat
    13921 gatggatctc gatttactga tttcgaactg ctgttagaac taaaaaatga aataatccga
    13981 ttaaaactac aggatgattt atcaatgaca aagtttcgaa aactagttca atccccaaaa
    14041 ctaccatcag ttgatacaat tatgaaaaga acgaacaaaa attgggagga attgatggca
    14101 gagattggat ttgattatcg aagaatcaaa atttataagc agagaaacaa cctatccaag
    14161 acaaagaaaa ctaaatagta tcactctaaa tcagacgttc aaaattaaaa tttggacgtc
    14221 ttttttatta cccaaaaacg aaaggagatt tattttatga aaaagccaag atttaagaat
    14281 tggcgtttgc tcgcaacact gagtctatta tgccaaacaa ttggtggatc gcttggccca
    14341 acgattgcct ttgctgatga aattactcat ccacaaacgg taactgtgga attggatttg
    14401 gctcaccagt atgctgtcga aggtacattt agcgatggtc gtcccatgtc agaagttacc
    14461 gttccgcact atgcagttta caatggtgtg aagcaagata ttttctgtat tgagccaggg
    14521 gtgccgattt acaatgagtt cactcctggt tatgagaaaa atccattgcc tgatatgtcg
    14581 gagaaagcga aattagtttc cgttttatgg aaggaagctg gtacggatgt ggatacacat
    14641 attgtggccc agaagatgat ttggcaagaa gttaatggct acacgcttca ctcaattaaa
    14701 cgttctgatg gtagtgcagt aaatatcgca gccattgagt caaaaatcaa tcaagccatt
    14761 gcggattatc aaaagaaacc aagcttccat aactccacag tgaaaaccgt gttaggtcaa
    14821 tcgactactg tgacagatac gaacggcttg aatttatcgg agtttgatga agttgtcgag
    14881 aatatcgcaa atattgatta tcgtgtgaac ggcaatcaac tagtgattac tccaaatgcc
    14941 aattcaaaag agagtggtga attaacccta aagaagtcag ctaatacagg aacaccggta
    15001 gcttataaaa tggctggtca acaaactttg atggcggggg caattgataa gcccaatacc
    15061 tacacaatca aaattgatgt ggaaactgag ggttctttga agattaaaaa agtcgataaa
    15121 gaaacaggaa atgttgtacc gggaaccgtt ttccatttag actttggaaa aacattacct
    15181 gcaaaagacg tgaccaccga taaagaaggc attgcgacat tggatgagat tcctcatggc
    15241 gcgaaagtaa ccattactga aaaatcagtg ccagctcctt atacgattga cactacacca
    15301 atcaccgcaa ccattaaatc tggtgagact atttctgtga cttcaaaaaa tactcgcgaa
    15361 aaaggtcaaa tcattttgaa taaaatgggg gtggaaacag gaactgatct ttggaacgac
    15421 aaatactcat tagccggaaa tacgtttgcc attcgcaaag acagtccaac cggtgaaatt
    15481 gtccaagaaa tgaccacgga tgaaaaaggt catgcggaaa caccaaaaga gattgccaat
    15541 gcgttggaat tgggaaccta ttatgtgacc gaaaccaaag ctagtaatgg ctttgtgaat
    15601 acctttaaac cagtaaaagt cgaattgaac tatgtcaatc aaaccgtggc tcttgttacc
    15661 agtaacgtaa aagggcaaaa ccaagaaatc actggtgaaa ccactttgac aaaagaagac
    15721 aaagataccg gtaatgaaac tcaaggtaaa gctgagttta aaggagctga atatactctc
    15781 tttactgcaa aagatggtca agcggtcaaa tggagtgagg cttttaaacc agagttagtg
    15841 aagggaacga aagcttctga tgaaacggtg actttgactt tagatgaaaa gaatcaagtt
    15901 gctgttaaac acttagcgat taatgagtat tactggcaag aaaccaaagc acctgaagga
    15961 tataccttgg atgaaacaaa gtaccctgtt tccatcaaaa aggtggatga taacgaaaaa
    16021 aatgccgtga ttacccgaga tgttacggca aaagaacaag tcattcgctt tggctttgat
    16081 ttctttaaat ttgctggatc agctgctggg actgccgaaa ctggcttcaa tgatctgacc
    16141 tttaaagtat cgccattgga aggaaccagt gagattacag atgccgaaga tgaagcaatt
    16201 acagcttata acgagcaact aggttttgat ggctatggca agtttgaaaa tcttccttat
    16261 ggggattatt tacttgaaga agtagaggct ccagaaggat ttcaaaaaat tacgccatta
    16321 gaaatccgtt ctacttttaa agaaaataaa gcggattaca ctaagagtga atacattttt
    16381 acgattaccg aaaaaggaca aaaacaacca attaaaacgg tgaccgttcc ttacgagaaa
    16441 ctgacgaata aagcattttc tgttagcttg aatcgtttga tgctctatga tttacctgaa
    16501 gaagaagata gcttaacttc tcttgggact tggaaagacg gaggcaaaga attgacagac
    16561 cttgattcta ccgagctagt tgataaattg agctataact tgcatgaaat caaagaagac
    16621 tggtatgtgg tagcgcaagc cattgatgtg gaagcgacaa aagctgccca agaaaaagat
    16681 gaaaaagcca aaccagtggt aattgccgaa acaaccgcaa cgttggcgaa taaagagaaa
    16741 acaggaactt ggaaaatttt gcataaatta accgcagaac aagttttgga taaaaccatc
    16801 gtcttattca actatgtgta tgaaaacaag aaaccctatg aagcaggtga tgatccagta
    16861 gcgaaagatg ctagcttgaa caatcaagcc caaactgtca attgtacggt ggaacgccat
    16921 gtttccatcc aaacaaaagc ccacctagaa gatggttcgc aaacctttac tcatggggat
    16981 gtggtggata tgttcgatga tgtgtcgatt acccatgatg tactggatgg ctcaaaagaa
    17041 gcttttgaaa caattctgta tgcattactt ccggataata cgaccaaaga aatttggaaa
    17101 tctggcaaaa ttgattatga agtgaatgac aaagaattca ctaaaaccgt acttgcggaa
    17161 aaagtggaca caagtaaata tccagaaggt acaagtttta ccttcaaaga aattaactat
    17221 ggcaaagacg gaaacatcaa tgggaaacac aatgaggatc taaaagaaaa aactcaaact
    17281 ttaacaccta aaaaagttcc gacaacacca agtgcaccag aacaaccgga aataccaact
    17341 gttccaagtg actctcaaga atctagtcct acagtaaaga cattcccaca aacaggggag
    17401 aaaaattcta atgttctgct gtttattggg tttaccttga tctttgcgac tgctgggtat
    17461 tatttctgga atcgccgaaa ctaaggtgat gtgatgaaac aactaaatag gaaaaaccaa
    17521 cgtgcgcgac cgcctcctaa aaaaagtggt cgcgctattt ttgtatccaa aactaaaaat
    17581 gaaaagagga aaagaagatg gaattgaaat ttgttgtgcc agatatggcc gaaacctttg
    17641 gaaagataag gtatgccggc gaaggcgaag ttttaactga aggatatggg cggaatacta
    17701 cagtaattgg tcgtagttat catttgtatt ccagtaagca acgagccgat gatattgaag
    17761 tggtggtagc tgcggaagcc ggcgaaaagg attttgatca agatcaacca ctaaaagccg
    17821 tgaatcccca tttggttgcc aaaggctatg agattgagaa tcgtgggttt accgactacg
    17881 tgttgtatgt cgatgattta gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag
    17941 gtattgttgt agattcagga ctaacgtttg ggaaattacg attttccgca ttacgccgag
    18001 aagtacggaa gcaaaatgag gatggaacgg ttagtaacga ggtaaaagaa cggacttata
    18061 atttgaaatc ctctgcgcaa ggtcgaatga tccaagttag tatcccagca aatgtaccct
    18121 tacgcgagtt tgcctatgac gcagaggtag agttggtcaa tccaatcatg gatacggtcg
    18181 ccaattatgt tttccgagaa ggaactactg tcaattggtt cattaaggcc gatgatttag
    18241 tcttgaaacg acaaccagat cagggaaatt ctacaaacca aaatgaagga aagaaatagg
    18301 tgaagcgtat ggaaattaat gttgaacata ttttagattg tctcgatcaa tatggaaaag
    18361 gtgagctaac ggaggaacaa ctcactaaag cattaaccta tgatgaaaaa atgtttctga
    18421 ttatgcatca agggttactg gaagtaggta acgatgccga agaagatttt gatgtactga
    18481 attggttagg ggaagaagag tccttcttta tggttatcga agtaaatgaa agtctatgtc
    18541 gagaagccga aaccgtattg gaagaaattg gtgtggaaat gccggatgcc attgaagttt
    18601 ttttaaagca actagtggaa acgaaacaac tccctatagc agtaaatgac tagcataact
    18661 gcttgaaatt ggatcaatga aatcctatga agcttttttc gattggaggt ggaaaccata
    18721 aaaatgtaca aagggcatcg tatacgggca ggagatcagc acttggttta tcactttgtt
    18781 cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt tcaagagctt
    18841 agacaatttg atatttctaa gctatcactt tctactatag aaatcgttcg ttctataaaa
    18901 gatttaatct atttgctagg aagtctcgtt ctttcaggaa gcacgatgtt gctttatata
    18961 cacttttttc aggatcattg gcgcagtttg tggcatcgac aaaagctagc ccggatgatt
    19021 ctagaaaatc attggtacga agtaaaacaa actcagagtg aaggattttt caaagacctc
    19081 aatagtagtc gaaccaaaga gacaatcagt tacttcccca aaatctatta ccgtatgaag
    19141 gatggtttac tttctattcg cgttcaaatt tcactgggaa aatatcaaga tcagctttta
    19201 aagctggaaa agaagttaga aagtggtcta tattgtgaac tagtagaaaa ggaactcaaa
    19261 gactcctatg tggagtacac tttactgtat gatataattg ccaatcgaat tgggatagac
    19321 gaagtagtgg cagaaagtgg ggctttgcga ttgatgaaaa atcaagtatg ggcttatgat
    19381 tctttacctc atatgttaat cgctggcggc acaggtggtg ggaagactta ctttttactc
    19441 actatcatcg aggctttatt aaaatctgat gccgagcttt ttgttcttga tcccaaaaat
    19501 gcggacttag ccgatttagg tacggtaatg cctcatgtct attcccaaaa ggaggaaatt
    19561 tctgcttgtg tggaagattt ctatgagcgc atgatgactc gtagcaaggc aatgaaagaa
    19621 atgcccaact acaaaacagg agagaattat gcgtttcttg gacttccacc aaacttttta
    19681 atctttgatg aatacgtggt ttatatggaa atgttaacga cgaaggaaag tgcggtgatt
    19741 ttgaataagc tcaaacaaat cgtgatgtta ggtcgtcaat ctggtttctt tctgattcta
    19801 gcttgtcaaa gaccggatgc gaaatattta ggagacggga ttcgcgatca atttaatttc
    19861 tgggtggctt tgggtcgtat gagtgaactg ggatattcca tgatgtttgg cgaggttgat
    19921 aaaaaattct ttatgaagcg tatcaaaggt cgaggctatg tagatacggg agggagtgtg
    19981 attagtgagt tttatacgcc acttgtacca aaaggatatg atttcttaag ggaaattagc
    20041 gacattggtg gattaaacgt taatacggaa ggataaaata atagtatgta gcctaggagt
    20101 gtcaagaatc ctgggctttt tttacgaggt gatttatgac atttttagat ttattcgctg
    20161 ggattggtgg cttttgctta ggtatggaac aagcgggtca ccagtgtatc ggcttttgtg
    20221 aaattgatga ttttgctcgc cagagttata aggcaatcca tgatacaagt aaggaggtgg
    20281 aaatgcatga catcacaagt gtatcagacg agtttattca atctctcgga ccagtggata
    20341 tcctttgcgg tggatttccg tgccaagctt tttcaattgc gggaaagcgg caaggatttt
    20401 ccgatactcg aggtacttta ttctttgaaa tcgctcggtt cgccgctatt ctccaaccta
    20461 agtttttatt ccttgagaac gtccggggat tactcaatca cgaaggaggg gctacgttcg
    20521 agacgatcct ccgaacgctg gatggattgg ggtatgatgt ggaatggcaa gtgcttaact
    20581 caaaggccta cgttccccaa aaccgtgagc ggatcttcct tatcggacat tctcgagacg
    20641 catgtaccga acaagtattt cctatcattg gatcgtctcc aacatctgat caaaacatca
    20701 gaaacttatt aaatatcaac ccttccaatc gaggaatggg gggacaagtc tatgggtcag
    20761 atgacgtagc gcctaccttg accggtgatg aaggaatcaa aatcgcattg ccggtagatg
    20821 atggaatttc tgtagcgggt atgttactgg gaaattttga acaaggcaat cgggtttatg
    20881 agataactgg aacagcgcct actttgtcaa cgaaacaagg cggaacgaag atcatgcttc
    20941 ggaaaaacga aacagaatat caagaggtga agccagggaa cagtgtgaac ctggcttttc
    21001 ctaattccac aagtcgtcga ggccgtctgg gaaaacaaag tgtgcatacc ttattaacag
    21061 gagaccaaca agcagtcgtc acggatcagt atcaaattcg aaagttaaca cctagggagt
    21121 gctggcggct tcagggcttt ccggactggg cttttgatcg agcttctcaa gtcaattcag
    21181 atagtcagtt gtataagcag gctggaaatt ctgtaacagt tccggtcatt tttgatattg
    21241 ctagaagatt aaaggaggta aaaacagatg atttatgatg agttgattgg ggaatctact
    21301 gggtgattgg caagattcag tcagatccgg aattggaaga agaactgtac cggctcaatt
    21361 ttgatattcg caaaaacggg gtgaaagtcc ctgatgatcc gtatgtaatt gatgaggaaa
    21421 cggatgctcg aatggagctg aatcaagtca tcagtgaatt ggaacgaatt gcggactttg
    21481 ctaaagaacc tgatatccgc caatacttgt ttgagataaa agcagagttg gaaattgagg
    21541 gtattaccgc ggagtaacgg ccaaataaac cactgatagg cactgaccgc gggggatttg
    21601 cgaagcagaa atcctccgtg gtgctgtgcc tgtttatgga cggcgttagc cggccatgat
    21661 gtctcacccc ccgtatctaa caggggggta caaaaacaca aaaaataagc aagcaacgat
    21721 aaggaatggc tgtcctatca aggataataa gcaatttgtg tagatgttaa ctgatttgaa
    21781 aaagttaaca ccttaaattt ggaggtgaga acttggcaca aaggaattta gattatcggc
    21841 tattaaaaga ccggcgtaat gaatatggcg tttcacaaaa taaattagct acgacttgtg
    21901 gacttagtcg cccgtactta aatcagatag aaaatggtgg cgtaaccgca tcgacaaaaa
    21961 ccatgaggaa aatttttgac caactggaaa gtttcaatcc cgagttacct ttaacgttac
    22021 tgtttgacta tgtaaggatt cgctttccca caacggatgc gaggaaaatc attcaagaga
    22081 ttctccattt gaaatttgat tatatgctcc acgaagatta cgccttttac tcctatcaag
    22141 aacaatatgt tatgggagat attgtggtga tgttgtcaca tgaagaagat aaaggcgtcc
    22201 ttttagaatt aaagggtcgt ggttgtcggc agtttgaaac ttttttactc gcccaaaagc
    22261 gtagctggta tgactttttc gaagattgtc taaaagccgg tggcgtgatg aaacgcttag
    22321 acctggcaat caatgatcga gttggcctgt tagatattcc tgatttaacg aaaaaatgtc
    22381 agaaggaaga atgtatctcc ttgtttcgta ccttcaaaag ctatcgttcc ggggaactgt
    22441 tgaaacctga tgataaagat ggtatgggca atactttgta tattggcagt cttaagagtg
    22501 aggtctattt ctgtttgtac gaaaaagact atgagcaata tatcaagtta gggattcctt
    22561 tagaccagac cgaaacgaaa aatcgttttg aaatccgact gaaaaatgat cgagcttatc
    22621 atgcaattca agatttgtta aaaggtcgca gtattgaaag taccacattt tccattatta
    22681 accggtactt gcgatttgcg gataaggtgg aaggcaaaag gcgaactaac tggcccttga
    22741 atgaacaatg ggggcgcttc attggacgga atcagaagga aattcaatta acttctgaac
    22801 cgaagcctta taccattgaa cgaacgttga actggcttgg acgacaagtc gcccctacgt
    22861 ggaagatggc caaggagttg gatcggttga atcagacaac gtacattcag gatatggtaa
    22921 ataatactcg actatcagac cggcacaaaa agattttgga gcaacaaagt atggcaattg
    22981 aaaatttgat tatatgaaaa gaggaatcgt gatggaaatt gtgaaagata ttttattggt
    23041 gtttggtggc agtatgcttg gtgttactgt gatgtgcttg atgcatgcaa gtgcagcggc
    23101 ggaccgagca atggaaaaga aaggaaagtg atgaaatgaa tttcggtcaa aatttgtatc
    23161 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
    23221 ttttaggatt taaacgcgag ttttccaaac tgattggctt cttggtgatt gctttgattg
    23281 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cttactcaat ttgtttaacc
    23341 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat acgtgtttat attgcgaacc
    23401 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccaccc gtcgattttg
    23461 atgaagtgaa agaacggatt ggattaaatg acgaatatga agagtacgca attcatgatt
    23521 atgaactgcc ttttgagatt gatgagtata caccaattga ggaaattaat cggttgtgtg
    23581 gtttagcgga agagcttaaa ggaacgccaa ttggtgaggt cgcttcagag attcaacatg
    23641 ccttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatatcatc tattatcccg
    23701 attgtaatga catgagtgat gtagcgtatt acttgattgt agaagctggt gatttaggcg
    23761 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtcga gacttggaga
    23821 ttggcgggaa ttatttggtt acgagtcgag gggtttttga actaatgtaa atcatttgtt
    23881 agaattgacc agaaatttct atcgtacttt tacttttcta tttgatatac tgaaatagaa
    23941 aatatttgtt gataggggtg ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc
    24001 atattcattc aactgcgagt cagtgctttc aggataaaaa ggttactgct aaggaatggg
    24061 tagaagaatg tttaaacaaa ggactcgatt gtgttgcatt aactgatcac aatacagggg
    24121 aaaatattga tgaatataag aaagtggcta tcgaaaatgg cttagatttt tttcctggag
    24181 tggaattaac gtgtggagaa aatggaactc atttactagt cctatttgat aaaagtgcat
    24241 gtacaaatga tatctctgat ttcttaacca agatgggtat aaaaagagag gattgggcca
    24301 gcgctgcagc ttgctcaatg aaatcagttt tagaagttat tgattttgct gagcacgagg
    24361 gcttgctcgt tattcctgca catatagatg agtttaatgg aatttcttca gcaaaatttg
    24421 gaaatctagg aaagatattt gaatcagaaa atatatatgc agtacagacg gttcaaaaag
    24481 aattttttga aaatcgctca caaatgattc cctcatctaa gcgtgacact atctatgata
    24541 gtgtgaatgc cagatatgat ggtagagttg gaaaagatac tttggaatct tggtataaat
    24601 cagttgttga ggcagaaaaa aataatatga catttcttag cttttctgat aacccacact
    24661 cgaaaggaaa ttcaaaacat ggactttggg gaataggcac aagatattcc tatataaaaa
    24721 tgaaggacga acctgacttg ggatcacttc gcgatgcttt aatgctaggc gaaactcgag
    24781 ttcattctga tttttctaat ttctccatta atgaaaatga agttttattg gaaaaattaa
    24841 gcttttctgg gaccactctt agcacaaaag aagttgtagt cgaattcagc gataatttaa
    24901 cttcaattat tggaggaaga ggaacaggca aatcttgtat tacaagattc ttggtctatg
    24961 tccttggaaa ggaagcagaa cttgatcaat tttctgaaat acaaagtgat tatcagaact
    25021 ttgcacaaat tgaacataat ggctctggaa tatttttgaa ggatacaatt gtgaaactga
    25081 atattttcta taaggggaca aaatatcaaa taataagaac acaggataga cattctattt
    25141 atgaatttac acaagagaat ggtctggttg aaaaagaaac agaacggttg agaatgattt
    25201 ccgataaggt gagtatatac tctcaaaaac aaatttatga aatttcaaaa aatcaaacta
    25261 gtatactaga attactagat ggatataact ctgatttgat ttcagagtat aaaaatgaga
    25321 ttgaaacatg tgtaaacgaa ataagaaaat taaattggga tattgtttct gtgaaaaaag
    25381 aaatacaaga taaggcgaag gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa
    25441 agctatctca taagagttat aaagatgttt atgatgagta ttcaaaagag acagatattt
    25501 atagggaact gaaacgtgat gtggaggcac ttaaggaaat tcccaaaaat ctaactgata
    25561 gtgttgacaa aattgacttt ggcaatggta taaaagttac agacgaaatt gatgaacacc
    25621 gcggagaatt gattaagaat ttagtgacca atcgggctaa gattcaaaca ataattaatg
    25681 aaatgtcaga tgaaattgat agttatagag cagagctgaa taaatcagat tggaaagtta
    25741 actataatga ggtttcaaag aggtatcaaa gaggtatcaa aaacttagtg aagagttatc
    25801 tcaagatgaa ttaaaaatgt tacaaaacct tgattcttta tatcaatctt tatcaaaaac
    25861 tgaagagcga ttggaagaaa ttattcaaca agaaataaag cttaaagatt accaagttga
    25921 ggttgactca gcaatgtcaa gaatgaataa atcatatcaa aatttctatt ctgagagaag
    25981 tagttcctcg cgcgaaattt tttctgatat tacaggaata gaggctacta ttcaaccaaa
    26041 tagggatttc aataattatg ttaaagagct cagagcttgt attaataagg aggacacttt
    26101 tgacagtgag tttggatctc ttattgataa gctgacaaaa aattccatta ctaatcaaga
    26161 tatatataat gacatacttg atataagagt tagtggggaa agtagtattt tttcagataa
    26221 aaaattaatc aattctataa gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct
    26281 taagccaaat gatttagtag ttatccgact tgaactaaat ggaaaaaaag ttagtttatc
    26341 gaatgctagt gctggtcaaa agacatctgc aattctttct atgattttgg cgtatggaaa
    26401 ttcaccactc atattggatc agcctgaaga tgatttggat agtcaattaa ttaataactt
    26461 aatcgtaaga agtatcatta caaaaaagga aaacagacaa attttaattg taactcataa
    26521 tgcaaatatc ccagttaatg gtgattcaga atggttgatt agcatgggag attcaaaaga
    26581 aatatcggct gatcagtgcg ggtcggtaga tgaacccaat atcaagccaa gaatatgtac
    26641 tgtgatggaa ggtggcgagg atgcttttac aaatagagcg aaacgctatg gttttaaaca
    26701 aattgtagaa taacactaga gggtgagaca tattattatg tccgcccttt ttaggaggaa
    26761 aaatgaaaaa aataaaaagc tataccagta tctgggcagt tgaaaaagtc atttacgcaa
    26821 tcaatgattt tcagttgcct tttcctgtga cttttaacca aatggcttgg ttcgttttat
    26881 ctttactgtt tgtcatcgta tttgcccatg tgcctccact atctatgata gaggaggtct
    26941 ttttaaaata cttaggcata ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg
    27001 atggcaagaa accactggga tttttacgat cctttatcag ctatcacttg cgatttaagg
    27061 tgacttttgc aggaaagaaa gtcaaagaac agaagaaacg atgggatgaa cccagtactc
    27121 ttgtaaggag tgtgaactat gtatccgatt aagtatattg aaaataatct cgtctttaac
    27181 caagaaggcg agtgttttgc ctactatgaa ttagtgcctt acaattactc ctttttgtca
    27241 cccgaacaga aatttcaagt gcatgataac tttcgccaac taattgcgca aaatcgtgaa
    27301 ggcaagatcc atgcgttaca aattgccaca gaaagtagta ttcgcgccac acaagaacga
    27361 tcaaaaaaag aaattaccgg tagactcaaa gaagtagcga aacaacgaat tgatatacag
    27421 acagatgcat tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc
    27481 tttaaactaa ttgcgacaga tgaagaagtc aatctaaaga gtctcaagaa atctttcttt
    27541 tctgggcttc aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc
    27601 ttatccaatg aagaaattcg ctgctattct aagcttgaaa aattaatgga aagcaaattg
    27661 gctcgaagat ttaaagtcag gcgagtcaca ccgagtgacc tgacgtattt gattgaacat
    27721 atttatggcg aaaaagggat accgtttgaa gaatacgaat ttcagctacc aaagaaaaag
    27781 ctaaaatcag aaacactggt gaaacgttat gacttattac gtccaaatcg ttgtttaatt
    27841 gaagaaaaac caaggtgctt acggatggaa catgagaatc atgagtcgta cgtagcgtat
    27901 ttaacaatta atacaattgt gggggagatg gaatttcctt cctcagaact tttctattac
    27961 cagcaacaac aatttacatt cccaatcgac acaagtatga atgtagagat tgtgacgaat
    28021 aaaaaggctt tagctaccgt tcgtaataag aaaaaagaac tgaaagattt agacaatcat
    28081 gcttaccaat ctgacaatga aacgaattcc aatgtcttag atgctttgga ttccgtggat
    28141 gaattggaaa ccaccttgga tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt
    28201 cgagtgagtg ccgaatcagt ggatgaacta aagcgacgct gtgatgaagt gctcgatttt
    28261 tatgatgata ccaatgtaaa actagtccgg ccctttggcg atatgatggg gctacatgaa
    28321 gaattcttac ccttaagtaa acgatatatg aacgactata ttcagtatgt tacgtctgat
    28381 tttttggcgg ggcttggttt tggggctact cagatgctag gagaattaga agggatttac
    28441 tttggctaca atgtggatac aggtcgtaat gtttacctga aaccggcatt ggcttcacaa
    28501 ggagtcaagg gttcagtaac caatgcgctg gccgccgctt tcctgggttc tcttggtgga
    28561 ggtaaatcct ttagtaacaa tctcttagtc tactatgctg ttttgttcgg tggtcaagct
    28621 gtgattgtcg atccaaaagg gggtgcgaaa cgttcctact tgaaaagagt aggcacagca
    28681 cttcattaaa tcaatattta atgtgtgttg gagaactaaa cacacgaata gtcgaatgac
    28741 ggggtaacgc cctgaaaggc tactttaata ctctgactgg cagaaacttg agtaaacaag
    28801 gctctgtcag aagctcggtg aagtcggcag acagacaccg taacagataa tgctgacgaa
    28861 agattcccag aggtgggcgt tgtgtcctat atgccggaag tctataaaat atctatggtt
    28921 agaatgtaga tgagaggtct actgacgaat ccttgaatgt acaggtctat atcggggctg
    28981 gatagaaatg tccagtgaca caaacagtgt cgctactgtg ggtgagtaag actccgcatt
    29041 tatgaaaatc catataacgt taaaggcgtt atcaaggtag caggcttata gaggaaacct
    29101 aaggatatat gtacagatag tgtgtttgga acgttgaaag ctgacaacat ggagatttta
    29161 ctgtctgtga agtgttcaca tcagaaagtt attgtgagat tagcaatgat ataaggtgtg
    29221 ttaatgtcag tgagagtggt ggcacagtac ctatgaaacc gtgataacaa gcggtggagg
    29281 gatagccact agtcatatga agaatagaat tactaagaaa aatggtatgg gttcgagtaa
    29341 gactaagaga tgtacttgct cctgcaaaca aggagggtac agaccatgct aaagaaaaac
    29401 aagctccgct ataacgagta ttatgatatg caacatattt atgacgagtt atacgcacag
    29461 agcaagaacg gtaataactt ttataagtta cttgaaatta ttggttcgga acaaaatatt
    29521 tgtttagctt accgtaatct gaaatcaaac agtggcagta aaacggctgg aacggatgga
    29581 atgacgatag acgatataaa acaactcagc aatgctgaga ttgttgctac cgttcgggaa
    29641 agccttagca actaccgccc caaatcagtc agacgtgttt tcattccaaa agctgggagc
    29701 gataaaatgc gtccattggg gattccgtgt atctgggaca ggctggtaca acaatgtatc
    29761 ctgcaagtcc tagagccgat atgcgaacca aagttccata atcattccta tggattccgt
    29821 gcgaatcgaa gtgcccacca tgcagtcagc agagtaacga cactgataaa tttatcaaaa
    29881 tatcattact gtgttgacgt agacattaaa ggatttttcg ataatgtcaa tcatggaaaa
    29941 ctattaaaac aaatctggac acttggtatt cgggataaaa gattgatttg cattatcagc
    30001 aaaatgctga aagccgaaat tgacggcgag ggtgtcccag aaaaaggaac gccacaaggc
    30061 ggtctgttaa gtccactgct ctccctcata gtccttaatg aattagactg gtgggtaagc
    30121 agtcagtggg aaacattcca gccgaagaac cgcagtaaaa atgggtggct tcaatacgca
    30181 aagaaatata ccaaactgaa aagcggtttt attgtacgtt acgctgatga ttttaaaatc
    30241 atgtgttcaa cctatggaga agcacaaaga ttttaccaca gtaccgtaga ttttcttaat
    30301 aaaaggctga aattagaaat cagtccagag aaatcaaagg tagtaaatct gaagaaaaat
    30361 tcatcagatt ttctggggtt caaaatcaaa gtcattccaa aaggaagaac gaaacacggt
    30421 tatgttgcca aaacagatat gaaccaaaaa gcattaaaga aagcgaaaac aaatctaaag
    30481 ttaaaggtaa aagatatagc cagacatacg actggcttca atatcagcag atataatctg
    30541 acagttatcg gtatgcagaa ttactactgc atagcaacta acgtatataa caacctgaca
    30601 gaagtaagtt acgctctctt accgaccata cgaatccgtt taaggaacat tgcaaaatca
    30661 gttccatttg aaagtacaag ttcagaattt cagagcagga caaaaggaat cagaccaaaa
    30721 acaaaaatag tgatgattgc ggataatccg ctattgccga ttcaaggagt acaacacaaa
    30781 aatcctatga atttctcgca agatatttgt aattttacaa agcaagggag aaacaaggtg
    30841 catgaggacg ttgtagttgt aacaaaagaa gaaatccgag cgttgttgga aaatgaaaac
    30901 cctgccgata gcgtagagtt taacgacaat cgtatttcgg catatatcgc acaacaaggc
    30961 aactgctatg taatgaatcg tcgaggaact ccgtccactc tgatatgtat tcacaaatca
    31021 gacaaaggag ataaccttga cagatattct aatctggcat ttgtggaaat tcccattgca
    31081 aaggcaatcc taacagaatc agcagacgaa gcaaaatcac tgttaaaagg ttatgttctt
    31141 aatagtcagc agaagaaaaa actgaacaga atacggacga attacggata ccagacaata
    31201 actgggaaat aacttataaa tctatttgga tatgatggaa cgccgtatga agcgaaagtt
    31261 tcatgtacgg tgtgaagcgg gggaaaactt ggagataaca tcaaaaagtt acctatcgct
    31321 atagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
    31381 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
    31441 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
    31501 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
    31561 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
    31621 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
    31681 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
    31741 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
    31801 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
    31861 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
    31921 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
    31981 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
    32041 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
    32101 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacggga ttacgtccat
    32161 ataattgtgt aaaagtgaag catcaaaaaa gccattgatc tcagttagga gtacaatgtt
    32221 ttttgcagaa aacatactcg aataggactg atatcaatga ctcaagtaca ttttactttt
    32281 gaaagcgaag aaattcaagc aattataaac gaaagcggtg cgaatgatac cgcaaaaaca
    32341 cttatgacta tcatgtttaa tcagctgatg gaagaacaac gaaatcaata tattcaggca
    32401 aatgcctatg aacgttcaga agaacggcaa tcacaaagaa atggttacta cgatcgttcg
    32461 tttacaactc gaattggtac gttagaactt catgttccac gaaccagaga cggtaagttt
    32521 agtcctacta tctttgaaag atatcagcga agcgaaaaag ctttgatcgc tgccatgatt
    32581 gaaatggtca tttctggtgt ctccactcgc aaagtaacta aaacagttga actgctaacg
    32641 gatggtgcga ctgtttctaa atcattcgtt tcaaatctga tgaaacagtt ggatccattt
    32701 gtttttgagt ggagaaaccg gagcctagaa ggctcggaat atccattttt catgtgtgat
    32761 gccctgtata tgaaagtaag ggaaaatcat cgtattgtct caaaaggtgt atatattggt
    32821 atcggcattg attctgatgg acgacgtacg attcttggtt ttgatgttca agacggcgaa
    32881 tcggaagata attgggatac cgtttttcaa tcgttcgttc aacgaggttt gttcggtgta
    32941 aaactggtca tttctgatgc acacaaaggg ttagttaagg ctgttcgcaa gaacttttta
    33001 ggtgcgagct ggcaaagatg ccaagcccac tttttgagaa atatttttga taagctgcca
    33061 aagaaagttt cttctgatgt aaaagacgag ttgaagagta tctttaaagc ctctgaactt
    33121 gaattgacac gtgaaagaaa agagcacttc ttggaaaaat atggttgtga ttcaaaatta
    33181 agtgctgctt gcgacatttt agaaaacggc tttgaagatg ctattcaaat tttgtctttt
    33241 cctgaaaata tccgccgaag gattcgtaca accaatgtgt tggaacggtt gaacgaagag
    33301 attcgccgga gagaacgagt gattcggatt tttccaaata tcaattccat cactcggata
    33361 attggaacac ttttgatgga aaaagacact gagtggctgg cttctccacg aaaatattta
    33421 gaatttaatt cgaataacat ttagcaccat gaacctgtgc gagtctcttc tttctgagga
    33481 gagacatcaa gagtcgctgc gctactgctc ctgacctctc tcctcagaaa gaaaggcatg
    33541 gcagttaggt tcacaacgca aaacattgtg cttttgatga ggttgaactt tta
//
LOCUS       DAJSVF010000025        32797 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSVF010000025 DAJSVF010000000
VERSION     DAJSVF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.026"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(60..1538)
                     /locus_tag="ON550_001173"
     CDS             complement(60..1538)
                     /locus_tag="ON550_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3954269.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1836..2771)
                     /gene="murB"
                     /locus_tag="ON550_001174"
     CDS             complement(1836..2771)
                     /gene="murB"
                     /locus_tag="ON550_001174"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR3954270.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2844..3764)
                     /locus_tag="ON550_001175"
     CDS             complement(2844..3764)
                     /locus_tag="ON550_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3954271.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3877..4629
                     /gene="xth"
                     /locus_tag="ON550_001176"
     CDS             3877..4629
                     /gene="xth"
                     /locus_tag="ON550_001176"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR3954272.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5493..6026
                     /locus_tag="ON550_001177"
     CDS             5493..6026
                     /locus_tag="ON550_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR3954273.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6516..7040)
                     /locus_tag="ON550_001178"
     CDS             complement(6516..7040)
                     /locus_tag="ON550_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954274.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7033..7506)
                     /gene="tsaE"
                     /locus_tag="ON550_001179"
     CDS             complement(7033..7506)
                     /gene="tsaE"
                     /locus_tag="ON550_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR3954275.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7629..8609)
                     /gene="pta"
                     /locus_tag="ON550_001180"
     CDS             complement(7629..8609)
                     /gene="pta"
                     /locus_tag="ON550_001180"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR3954276.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8762..9442)
                     /locus_tag="ON550_001181"
     CDS             complement(8762..9442)
                     /locus_tag="ON550_001181"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3954277.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9568..10437
                     /locus_tag="ON550_001182"
     CDS             9568..10437
                     /locus_tag="ON550_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3954278.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10463..10978
                     /locus_tag="ON550_001183"
     CDS             10463..10978
                     /locus_tag="ON550_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954279.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11025..11618)
                     /locus_tag="ON550_001184"
     CDS             complement(11025..11618)
                     /locus_tag="ON550_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954280.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11643..11822)
                     /locus_tag="ON550_001185"
     CDS             complement(11643..11822)
                     /locus_tag="ON550_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954281.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11899..12408)
                     /locus_tag="ON550_001186"
     CDS             complement(11899..12408)
                     /locus_tag="ON550_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954282.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12504..12884)
                     /locus_tag="ON550_001187"
     CDS             complement(12504..12884)
                     /locus_tag="ON550_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3954283.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13214..13963
                     /locus_tag="ON550_001188"
     CDS             13214..13963
                     /locus_tag="ON550_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR3954284.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14147..15424)
                     /locus_tag="ON550_001189"
     CDS             complement(14147..15424)
                     /locus_tag="ON550_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954285.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15503..16147)
                     /gene="pcp"
                     /locus_tag="ON550_001190"
     CDS             complement(15503..16147)
                     /gene="pcp"
                     /locus_tag="ON550_001190"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR3954286.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16346..17290)
                     /locus_tag="ON550_001191"
     CDS             complement(16346..17290)
                     /locus_tag="ON550_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR3954287.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17292..17978)
                     /locus_tag="ON550_001192"
     CDS             complement(17292..17978)
                     /locus_tag="ON550_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR3954288.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18452..18772
                     /locus_tag="ON550_001193"
     CDS             18452..18772
                     /locus_tag="ON550_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3954289.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18926..19831
                     /locus_tag="ON550_001194"
     CDS             18926..19831
                     /locus_tag="ON550_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3954290.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19828..20601
                     /locus_tag="ON550_001195"
     CDS             19828..20601
                     /locus_tag="ON550_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3954291.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20782..22206)
                     /locus_tag="ON550_001196"
     CDS             complement(20782..22206)
                     /locus_tag="ON550_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3954292.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22417..22863
                     /gene="nrdI"
                     /locus_tag="ON550_001197"
     CDS             22417..22863
                     /gene="nrdI"
                     /locus_tag="ON550_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3954293.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22928..24910)
                     /locus_tag="ON550_001198"
     CDS             complement(22928..24910)
                     /locus_tag="ON550_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3954294.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24924..25820)
                     /gene="murQ"
                     /locus_tag="ON550_001199"
     CDS             complement(24924..25820)
                     /gene="murQ"
                     /locus_tag="ON550_001199"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3954295.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25823..26884)
                     /locus_tag="ON550_001200"
     CDS             complement(25823..26884)
                     /locus_tag="ON550_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3954296.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(27095..27661)
                     /locus_tag="ON550_001201"
     CDS             complement(27095..27661)
                     /locus_tag="ON550_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3954297.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(27689..29191)
                     /gene="eat(A)"
                     /locus_tag="ON550_001202"
     CDS             complement(27689..29191)
                     /gene="eat(A)"
                     /locus_tag="ON550_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3954298.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29449..30570)
                     /locus_tag="ON550_001203"
     CDS             complement(29449..30570)
                     /locus_tag="ON550_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3954299.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30696..31637
                     /locus_tag="ON550_001204"
     CDS             30696..31637
                     /locus_tag="ON550_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3954300.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHADLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31787..32683)
                     /locus_tag="ON550_001205"
     CDS             complement(31787..32683)
                     /locus_tag="ON550_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3954301.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         9550 a         6794 c         5741 g        10712 t
ORIGIN      
        1 tttgaaaaaa acataaatca gctaataaat atgtgagaca aagctgccat ccataaatac
       61 tagctgattt tttcagttaa atgtctattt ttttctctga tgatggtatt tccaaaatat
      121 tcccagtcat ctggagtcgg tggatttttc caaattatct gttctttatt aaatttttca
      181 agtaaacaat cagataaata aatattcgta cgagaagtga ttcgactctc aatcacaata
      241 ttgtaatttt taaatccttt cacttgagaa ataatgtaat cggtataagc agatccttgc
      301 gaaaaatcaa tacagacatg aatcggtgct tcaaaatatg ataccggaca aataccgacc
      361 aataaaaaga gataatcata aaataagcga atcatgaaac gttcatcttt tcccaagaaa
      421 tagcgtttat tctcttgtat gaccttttgg attgctgtac tgaatattgg atacgtctcg
      481 aagaaaaact gtagctgctt tttagacgta aagctagtca tctcaaagtc gaatatccaa
      541 ttttttcgat tgacatgcat catacccata cgaagttctt ttcgtatata tagtctttct
      601 tcttcagaga tttcaatcga aaatggtaat tgatcaatca attccactga cattttttga
      661 ctaatcatgt ggatatcatg aaaatgagct tccgcaagtc caaatgcgcc atctaattca
      721 aattcttctt ccgtgatcaa aaacaacatg atataagaaa tctcatcaat gatctgctct
      781 ttagggatat gaagaaaaga aatatgtgat aaatccttga tgatcttcat tccttctgag
      841 ctcttaaagt attcatctga aaagaaacgt tttccgattg gttgattccc ataggccctc
      901 aacaaactaa tagtggttag caaattgaat ttgattcgtt ttgttgcaga cagttttaaa
      961 ttgaacagat attcaaaata ttgcacgata ctctttgctt cattcatgac gctttctcta
     1021 aatggcatag aaaaaccatt aaagaccccg aaatataccc caaataatac gttccgtatg
     1081 tttatttctt ccccaactaa tatgtttttc tttaacttta tttgatgctg ttcaagaaaa
     1141 gagactaatt ctcttctttt tacataggct cttgatatgc ttaaaaaatg attctctgca
     1201 aatttttcga tcgttacttt tgaatccacg atctccgtta acagtcgagc aatttttgat
     1261 cgttgaaagt agattcttct taaatttttg atatccgtca aattcagacg ttcgacatgg
     1321 atctctgctt cttctgaaat ttggatctct ggctttgttt tgaattcagt aaattcttta
     1381 tttaattcta tgatataatt tcttgtttta aatttagaca aacctaatgt ttcatataac
     1441 ttaggcagca ctacataatt ctttttctca aattctaaat atctagctag cagcagtttt
     1501 tctctatctt cctcttctat aaggtaatcc aacttcatat cctacacctc aaaaaatgcc
     1561 tcataccaat tacttgatat gcagatgaaa gttcatcttt tcacctatac tgattttatg
     1621 atggattatg cttgtttttt catcagttat tctcattttt tatctttttt ttgagaatat
     1681 tgcaaaaaat ggaaaaattt atgaggaaat attctatatt ttttgtttta ttgtgttaaa
     1741 atgtacaaga tttgcgccat ttgcgtaaac aaccacaaaa aagagcagga cggccaccgt
     1801 cctgctcttt tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt
     1861 tctgatattg cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat
     1921 ttttctttga ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca
     1981 tgatcgatat tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc
     2041 aggccttgca atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta
     2101 aagacacttc cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac
     2161 tcatccattc ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc
     2221 aatacgatag cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct
     2281 tttgtcaaag ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct
     2341 ttgatctctc cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg
     2401 atgccgcaag caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca
     2461 atcagtgagg cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt
     2521 tcagtcagca tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca
     2581 tttcctaata ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact
     2641 tcttcttgtt ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta
     2701 tagttcatca atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct
     2761 tctttattca ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct
     2821 taatacaatc ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc
     2881 tcacataatt catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta
     2941 cctaaacgga tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc
     3001 gtgtttgtcc agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc
     3061 tgggtcagat cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata
     3121 ttagcgccac ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt
     3181 aaaatggcat attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg
     3241 ggttcatcta ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg
     3301 aactctgtcg tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct
     3361 gggatacgtt tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa
     3421 attatttggt tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc
     3481 agtggtttgt caatataaac atggatacca ttttctagac attttttggc taattcataa
     3541 tgtacttttg ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg
     3601 atcgtttcat atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta
     3661 tttcttgttg caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat
     3721 gctttttctg cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga
     3781 tcatttagtt actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca
     3841 aaggtaagga aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta
     3901 aacgggcttc gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc
     3961 gattttttct gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca
     4021 ggttattatc aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt
     4081 gctaaaaaac cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa
     4141 ggccgtctga ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat
     4201 tctcaaagcg aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat
     4261 tatttggaaa atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct
     4321 catcaaaaga ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca
     4381 gaagaaagag cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat
     4441 ttttatccaa cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa
     4501 aacaatgctg gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta
     4561 gctgatgcaa agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat
     4621 ttgaaataaa gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga
     4681 aaatggcttt ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca
     4741 ctgtttatcg agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca
     4801 aaaatagttc ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga
     4861 acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa
     4921 acaaaaatag ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
     4981 tgaacaccgt ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc
     5041 caaacaaaaa tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc
     5101 ttttgaacac cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa
     5161 acaaatggca aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc
     5221 tgattgaaca ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga
     5281 aatttttttg aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc
     5341 atgatcccca tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca
     5401 aatattacat aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg
     5461 ttacagtaat ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa
     5521 ctgccaatca tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc
     5581 agatcgttga tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg
     5641 tccaaattca cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct
     5701 ggcaaactat ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt
     5761 ttgatacaaa ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc
     5821 tttctttatg tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat
     5881 tgaacactgt gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag
     5941 atagtcgtgc atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat
     6001 tgaaaaaact tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga
     6061 gcctgccgtt tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat
     6121 taaatattgt gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg
     6181 tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
     6241 tttcatttgg ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg
     6301 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat
     6361 attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa
     6421 aaaatggact gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa
     6481 aaggatgaca gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta
     6541 tatacatcta aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt
     6601 tggaagccca tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc
     6661 tccaatcgaa aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga
     6721 gtgcctagtc ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga
     6781 gacaaacgaa attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag
     6841 attgcaagca gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag
     6901 gtttctggtg gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta
     6961 acttgtccca tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg
     7021 atcccaactt cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt
     7081 ttcactttgc tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact
     7141 tttttctaaa atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca
     7201 ttcaacaacg gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct
     7261 agctgctacc cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct
     7321 aataatcgtg tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc
     7381 tttcgtcaac gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg
     7441 cattgcggaa tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc
     7501 gatcattttt catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt
     7561 ttttttaaag aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg
     7621 tgtatttgtt aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct
     7681 tcttcatttg ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt
     7741 ccgattgctt caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc
     7801 aattctggaa atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt
     7861 tgtgcaactg aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt
     7921 ggcgctagtt ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc
     7981 gctgagcctt ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct
     8041 gctgtttttg cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg
     8101 atcgcacaat ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct
     8161 ccgcttgtcc gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg
     8221 tcgccagtcg aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc
     8281 atcgtaccaa agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt
     8341 ccatttctac gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg
     8401 ctgatgatcg taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct
     8461 tcttcgttcc ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg
     8521 cctaaaatac gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt
     8581 ttaaatctta aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt
     8641 atcatacact attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct
     8701 ggaaaaatta agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt
     8761 attacactgt ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat
     8821 cattcgtttt cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg
     8881 gatgtggaga tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg
     8941 ccggttttcc ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga
     9001 tcgtgtctgt taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa
     9061 cggtaagaac ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat
     9121 gttgaacagg tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg
     9181 gcgggatctt tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat
     9241 gataaggatc ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg
     9301 cttcatagat atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa
     9361 attctcttag atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac
     9421 tgttatgaat gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt
     9481 cttcaatcac aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac
     9541 aagaagcaga aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc
     9601 acattattag atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca
     9661 aatgatgccg gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca
     9721 gctttagatg aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt
     9781 gataaagaag gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt
     9841 ttagatattc tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat
     9901 tctgaaaatc aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct
     9961 caccttacgt ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc
    10021 acatacgtgg acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt
    10081 atttgttttc atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt
    10141 ttaggcgatc tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac
    10201 gctcaaaaag gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa
    10261 gtcatgacga ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt
    10321 ttcgcaatgg gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca
    10381 aaccttgaat ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt
    10441 tatctagaaa agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg
    10501 tgcaactcgg acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg
    10561 ctggggaaca aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca
    10621 catcaagcag ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa
    10681 ggtgggaacc atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact
    10741 tcactggaca gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca
    10801 gcaaatcatg aaaatggata aagcaacact tttttcagga gattactatg tattggatgt
    10861 ctcaaatgat gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct
    10921 ctaccgtaaa gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa
    10981 agatgtacct gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc
    11041 atatgatcat aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt
    11101 tcgtataatt ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga
    11161 ttagctttct tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct
    11221 ctaaaatctt ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta
    11281 tcaatgaaca aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc
    11341 tgatcgatga ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg
    11401 tctatttctt ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct
    11461 tcttctaaaa tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata
    11521 tcttttagaa tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa
    11581 cgaatcatag tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat
    11641 cgttatttta aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt
    11701 gccgcttcag ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact
    11761 acattattga cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc
    11821 atgttatctc aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag
    11881 agaagcaata tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat
    11941 taatcggact ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt
    12001 tgcttttttc ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc
    12061 ttccgttttt tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt
    12121 tgttttccca gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc
    12181 atatgttgca ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat
    12241 gttatgttct tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata
    12301 ttcctggtca ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt
    12361 aatccctatt acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc
    12421 tgtttctttt ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa
    12481 aaaaagtgac aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca
    12541 aagaaaaacg tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc
    12601 cctaaggact gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga
    12661 cgcaagccac aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc
    12721 tccaattcag cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa
    12781 ttttctctga ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa
    12841 tctgaaacaa tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc
    12901 ttcgtttaag ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa
    12961 aaaatagctg tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc
    13021 caagacactc ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa
    13081 aacaaacaga aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca
    13141 agaaaagcat acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa
    13201 ggagtcctca tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc
    13261 gcaacaaaat tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa
    13321 tagtcccatc acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg
    13381 tattttatcc atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat
    13441 ttgttttatt atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc
    13501 ttatatgatc acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc
    13561 ttcaaaaatc cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct
    13621 tgtctatctt gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt
    13681 agatgccatt gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa
    13741 caaccaatat tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc
    13801 atttacgcat ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa
    13861 tgatgtcctt gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca
    13921 aaaaatggaa gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga
    13981 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag
    14041 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
    14101 cttcttattt cttggagtta aacgcttctg tcccagcctc tattttttat agtaattcct
    14161 tcagatactt tcttgcctca tcattgaaac gcagtgattc ttctaacttc tgataatatt
    14221 ccaacgcttg tttctgagct tcagtaaaac gataatgttt tcctttccat ttgtcgattg
    14281 tctcttgcac atttccagat tggatatctt tcactactag ttgccatttt tcttttgaga
    14341 ctgtacggtc tttcttatca gcagattgta tagcttctcg ttctttacgc aatactttta
    14401 aaaaaccatt tctgactatc cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc
    14461 gacaatattg cttccaaata aatagataga tttttggttg gatgaatcgt tggtttttcg
    14521 caaaatattc catctgagca gtgacttctg gtgttcgttg acgttttgtc tttctcaaca
    14581 cggaaataaa tgtttcaaaa taagcattag gatgtactgg cacttcttca tatacgatgg
    14641 attgccaatc tttacgtaaa aatgcgcgat agttgatcaa gaattcttct acatccttta
    14701 ctaattcttc tgcaaagtcg tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc
    14761 cattgatgtg ttcacttatt ttcttgattt cattttccaa tgcttgataa tccgctatgt
    14821 aaatcggcat ttctacttgg cgaaattctg cgagccgctg agctaatttt atatccggta
    14881 aatatggctg tttcatccga cgattttggt acaagacaaa acagtatttt tgccatagtc
    14941 cttcaggaaa atcgatattc ttctgcttaa gccataataa ttcgtctaag gccagatcaa
    15001 cttttgtcat tccctgatgg atcgaagctg ccactgtcgg agagacatgc aggtgatcgg
    15061 caaagtggtt gatgcctagt tttaggatct ttccggtttt tggcgattta acgatgtatt
    15121 gcacttttaa ttctctacca cactcacaat acaatttttg ctggcttcgt tttgttcgaa
    15181 aaaacggatt gatcttgaag tcagaaaaca cccaatctgt tgcagctaaa taatgttctt
    15241 ccataaaacg agagctgatt agatatcggc ggtgcttatc gactagttcc cgctgttttt
    15301 tcgttaattt tgcatagatc tcccaacgtt gactgcttgt gtattgttta gaaattttga
    15361 tgggatagct tttgctgatt tcttttggaa accattcttc aaagacggat tgccattctt
    15421 ccacggctgt ttctttcctt tctaattaca aaaagctggc tactaacata acgttagcaa
    15481 ccagccagtg tttcttgcta aattagtgag ttgttccggc atttccggaa atatcttctt
    15541 tattttctac tgctgcttca actgcataag tcaaagaaga agcaatgtct tctaaagaca
    15601 tagaaggcgt acccactgga cgatcgatca cttgatcggg tgagaatggc acgtggatga
    15661 acccacctcg aattgccggg aattgtgttt cgatcaaatg aagcaaacga tacatgatat
    15721 cattgcagac aaatgttcct gctgtataag agatgtacgc tggtagacca tgatcttgta
    15781 cattttttgt cattgctttg attggcaatg aggtaaagta agcggctgga ccgttttctt
    15841 ctaaagccgt atcgaatggc tgatttcctt cattatcagg gattcttgct tctgctagat
    15901 tgattgcgac acgttcgaac gagactgctg accgcccacc tgcttgtccg acacagatca
    15961 cgacatctgg ttggtgctgt tggatggctt ctttgactac ttgactgctt ttttcgaata
    16021 cggtcggtac ttccactttg atgatttcag caccgctgat ttcatctggc atttttttta
    16081 ctgcttcata ggctggattc actttgtccc caccaaatgg atcaaaacct gttactagta
    16141 ctttcatctg ttgttcctcc gtccgattga tttaacatat ggttatcttt tatcatacca
    16201 ttttccttga acagcgtaaa taaaggaaac aatagtttaa ataacataac agatcaaact
    16261 taatatattc ttataaaaaa atagtgttcc actacaatgt tagaagaggt tgcgacaatt
    16321 attctgtcat aacccctctc ttctattatt taaatagaat catataagca atttggaaaa
    16381 ctagcataaa taacgcaata aacaactggt ttttgatcac tccgtatttg tctttcatct
    16441 ccagcatcgc tacaggcacg atattgaagt tagcagccat cggtgtcagc agggtcccac
    16501 aaaaaccgca agtcaatgca accattccta ttaacgctgg attcgctcca tgactaaata
    16561 cgaatggtgc accgatccca accgtcatca cggtgatggc cgcaaatgca ttccccatga
    16621 ttaccgtaaa tatcaccatg ccaagagcgt aaatgatgat gccgatgttc acatttcctt
    16681 ctggaacgat cgtacctact gcattggaaa tcactgtccc aactccagcg cttgtaaata
    16741 ccgcacctag agaagctaaa agcattggca acatgcttaa cggccctact gtgcccagca
    16801 tatctgctgc atcatctaaa aatgtacttg gtcgattatc tcgagaaaag aacatcaaga
    16861 ttagcatggc tgcaaaaaca ccgacgccca cgccgactaa agcacctaaa ttggtaaata
    16921 gagcaaacaa gatcgcaaag acaccgatac tcaaagctgg aataaaaatt ttcattccta
    16981 gtttgtctga cattttttcc atatattctt ttgatggaag ccggctttct cctttgctga
    17041 ctttcttagc aattgctggt atggtcatcg caaagatcaa gccaccgttc acgattgcgg
    17101 gaagccatct tcctcctgca ataactaccc ctaagaccgt ccagaaaaag gctgacccat
    17161 aacgatgagg attggtttta tctgttaaat ttcttactcc agtgtaaatc aatacgagtc
    17221 ccatcacgat ataaaggatc tccagtaatt tatcgcctaa taaaatatca ggatttgtaa
    17281 aaaagtccat cttattgccc ctttcctgga taatatttct tcgctaattt cttttcttta
    17341 agatagaagt agacacttgc cacaactaaa gccgtgatag ccactggaac ttccactaaa
    17401 gcaagctgtc ccaatgttac gtcatatcct agatctttca gtgttgattg gactagcaac
    17461 gcccctgctc ctccgatgaa tagtacttgg ccaaagaacc agcagacatt ttccatagct
    17521 gaagccatac cttttagttc ttcttcatgc ttaggatgga cttctaaccc ttttgattcg
    17581 atggtcccca atgccatcgg taaaatgatt ggacgaacaa atccagcaac tccaccaaaa
    17641 ctcacgttaa aagccgcaaa gatcattcga aagatcccat aaatatctac aatcatacca
    17701 gcagatactt tcttgaatcg cttgatcaag gaagccgctg attctttcag accatttctt
    17761 tcaagcgttc ctgttgccaa cataatgatg ataaaaatcg ccatcccgcg attgtttaca
    17821 aaacttgtac ctaaaagttc taacattcct tgaatgccaa gaccgccagt caaagctgta
    17881 acaactaaag caaccattac gatcaatatc gagtctaatt taaatgcaaa cccgagaata
    17941 acaatcaaga cgcctaaaag gctaagataa ttttccatat attttcctcc ataaaatgac
    18001 aatatttttt taattataaa gcaaaacaca taaaaaatat agttgttttc tcacgaaata
    18061 gacactattt ttaaatattt ttcgttattt ttttatcaca aaaattatct tacttattgt
    18121 aaggtaaagc tttttttaat ttattgcccg ataatatata gaaataaggt ctagttactg
    18181 ttttttttaa atgattaata aataacaact tctttctatg aaattgcaaa gcattcatct
    18241 tcttttccgc taacgatata tccgtcagtg cttggtacac tttactaaaa agtatgagat
    18301 tgccgatatc tggataggtg tgctatacta cagaaaaaga tatacaatct gccaccgggt
    18361 aggataaaga ggatgatcat tccattaggt ctttgaaggg atgattttcc tctttttgtg
    18421 tattttttta tagttaagga ggaatgtaag tatggcatgg atggaattaa tcatagcagg
    18481 aattttggaa gtattctggt caacgatgat gaaatggagt gatggttttt ctaaaatcaa
    18541 ttattctttt tacacagtga ttggcatgat tgctagtttt tactttttgt caaaggcaat
    18601 caaaagtttg ccgatgagtc tctcttatcc tatctggaca gggattggcg cagttggttc
    18661 ggtcataatt ggtgtggtct tgtttcatga taagctgaat ctttccactt ggttctttgt
    18721 ggggatgcta ctcatcagta tcattggcat caagattact agcggccact agacacttgg
    18781 aataagtact gatcagaaaa aataagcctt tttatttaat attcaatttt tttatgaaaa
    18841 aataagaaat aagtccaatg acctagcgac ttctccactc atcaggtgta tgcttgtact
    18901 atctatagaa aaggagtgaa tatttttgaa aagtctttcg gtccaagaat tatccaagaa
    18961 ctttggtcac aaacaagccc tcagcgatgt cagttttagc ttatcccctg gcaaaatcgt
    19021 tggattagta ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta ttttaggact
    19081 gatccattac tcagatggaa caatctgtat tggtgaaaat gagatttctc cctctagtca
    19141 taaagggttg gaacaagtag gagcattgat cgaatacccg ggtatctatc cattcttaac
    19201 aggctatgat catctgaagt tattctctga aaccaacgat gtatctgcta tcgacactat
    19261 cgtgaatcag ctcaagatgg aaaagtacat tcacaaaaaa gcgaaatcct actcgttggg
    19321 aatgaaacaa aaattgggaa ttgctcttgc cttactgaac catccgtcta tcgttatttt
    19381 agatgagccg atgaacggtt tggatccaca agcaacaaga gatgtgcgaa aaatcattac
    19441 cgacttggct actcagggca cctctttctt gatctccagt cacttactca gcgagcttga
    19501 aaaaatggtc gatgagctga tcctgatcaa tcaaggaaag atcatcaaac aatgtacaat
    19561 ggctgaattg aaccaaacag cgcaagattt catcgttatc tctacaacaa atgatctagc
    19621 tgctcgaacg atcttgacag aagctggata tactttggaa gatcctgata aaatcaagct
    19681 tttaaaagaa aacgaagaat tattaggaac cgtgatccag ctattgtctg cacaaaatat
    19741 ccatgtgaca gacgtccagc atctgacgaa tgatttggaa tcttctctat tggaaattct
    19801 ggattcagta aagggggaag aagaaaaatg acattgcttg taaaacaaga attatttaaa
    19861 ttgatcaaga aaaagtcaac tgctgtttta tctgttctat tagttgtttt gctcatagga
    19921 acagcacttc tagccaaaaa atacacgacg atcattgatc cagttgaaat gactgctcag
    19981 ctttttagtg ccacatcttg gattgtcttt atcatgattg cagctgcaag cacgatcatt
    20041 tcgatggaag cacaatatgg tacattgaaa aatctccttt atcgaaagta ttctcgagga
    20101 gaaatattag taagcaaatg gatcacatta gttatttatt ctgtttattt atacctttta
    20161 gctattattg tcactgtgtt gatgaaactg attcttttcc cttcgatttc cttcacggag
    20221 aaggtttcta ctggtcaaac actgatccaa agcttgttca cttatacttt gggtagttat
    20281 atcggtttat ggctgatttt aagtttagtg ttgatgattg cttgctttat caatagttcc
    20341 ggcgcatcca tctcagcagg aatcgtcttt tattttgctt cgtcaatcat atctggtatc
    20401 cttattgcac tcatccaaaa atgggagtgg atcaaatgga atccgatcag tatgttgaat
    20461 ctgcaaaacc aaattggaaa cgaagagatc atgaagcaat tgactcatct ttctactaat
    20521 caaatgctat tcggtaattt agcttacatc gttttgttct tagcattagg atatctcgta
    20581 tttaagcgaa aaaatgtatg atgactcatc aaaaaagagg ctgtgaaaga gacgttcagc
    20641 cttattgccg attggctgtc tcttaaacac cgtttattct gaaaaaggag ctgtgacaac
    20701 atttgtgtca cagctccttt cctagtatgt ttttgaataa acgacaggct cacaacctct
    20761 ctttatactt cttttatact tctatacgtg aagcatccca gtggcaaata aaaccattgc
    20821 aatcactccg accatcgtca cacctataag taaaatctta tctaagtgga tatgttgtac
    20881 aggctcgttt tgctccttac gtgctttgag gtaaaaataa atgcctggca agaaaagaac
    20941 aagaacaagc attacttcat taaaggctac gactagcata gcccatactt gaaaggcact
    21001 tgctaaaagc cctactaaaa attggaccca ttctttccgc tgataagaaa gtttcatttg
    21061 gtacaatcct aagaaaagcc acgtgaagaa aatagctgct gtaccaagtg tgtaagcaaa
    21121 gttataagca ctgtcagtga ataataatga aagtaaaaag acattcgtac ataacgtcgt
    21181 caatacgata gcataagttg gtgccccatt tttgttgagt tttccccatg ctttaggcaa
    21241 aagtccgtct tcagccatgt ttcccattgt ttcaatcggt aagatcgtcc atgagagcca
    21301 aacaccaact aaagaaatga tcaaaccgcc gttgatcacg actgctcccc atttaccaac
    21361 catcatttca aacacatagc ccatagcagg ctgcgccttg atcgtttgca gctgattcat
    21421 cgtcattgtc ccatatggaa gcatggaaac caagacgtaa ataataatca agccgaatat
    21481 ccctagaatc gttgctttcc caacatctga ttgttttttt gctcgtgaac ctacgacaga
    21541 ggctccttca ataccaacaa atacccaaag catcaccatg atgcaactca atacttgact
    21601 ggaaaacggg atcggctgtc cattcgcatt caatataatg tcgctagaaa agttatcaat
    21661 gaattgttgg atcgaaaacg tgctgatagc tactacaata aataaaaata atggcactaa
    21721 tttacagaat gtgacgatag cattgacgaa actagcattt tcgactcccc gattcactag
    21781 ccatgcatat ccccataaaa agactgtact catcacgatc gatgccagat tttgcccacc
    21841 ttcaaatact gggaagaaat atcctaatgc gctcatcaac aatgttgcaa aagctacgtt
    21901 gcctaaccag catgtgagcc agtaacccca accgctgata aaaccagcga aacggccaaa
    21961 accttcttct gcgtaactaa agatcccgcc ttttaaatct ggacgttttt tattgatatt
    22021 attgatcgac aaggagagca tcaacattgc tgttcctacg agtacccaac tgaaaatagc
    22081 tgggccgcca gccgatccgg agatgtccgt cataatacca aatacgccac ctccaatcgc
    22141 tgaaccaatt actaaggcag caagtggaat taaaccaatt ccttttttct gttgctctgt
    22201 atccatgtaa gcatcctcca aaataaaacc gcttttatca aaaagtataa caaacctttt
    22261 ttatttttac ttaattaata aaaactttaa tagaaatata attctaccgg cttttttctc
    22321 attatacaag caaagcgcct agtccctttc caaattacac caccaaaaaa cttaaaaaag
    22381 atgctatact aaaaggaaag aattggagga tgatccatga acattttata tatatcgatt
    22441 tcaggaaata cgcgtgcctt tgccaaacat ctagcagaat atgctgaaaa aatgcatgca
    22501 gaagatcctg tcaatcctga agttacttta aaagaaatcc atgaaaatag tgattttacg
    22561 aaagaaacag aaccattttt tactttcgtt cctacttatt tagacggcgg aaatggttta
    22621 gataacggtg atacagaaat cttgaccgaa acgatgcgtg aatatctgga acatgaagac
    22681 aatcataaac tttgtcttgg tgtagtcggt agcggaaata aaaacttcaa taatcagtat
    22741 tgtctaacag ccaaacaata tgcacaaaca tttggctttc ctttcttggc agattatgaa
    22801 ttacggggaa caccatctga tgtgacacga atttatcaga ttttagcaga aaacaataaa
    22861 taataaaaaa gaagaagctg aaaaatagat ttcagtttct tctttcgttt ctattgtttc
    22921 tatcatatta agctttgaca tccatcacag gtgcttttgc ttttgcttta cctgtttttt
    22981 ccaagacaaa gttttttaca cgatccatat tggttaccaa gacgatcatg gatgtttctt
    23041 tgcctgctgc tttgatcgct tctaagtcag cttctgctac aagcgtatca ggtgtcactt
    23101 gctgcccttc tgttactttc acatcaaatg gcgtaccgcc taagtcaact gtgttgattc
    23161 ccatatgaag caagatttct aaaccatttg gcgttgtgat cccaatagcg tgtttcgttg
    23221 ggaagacaga gctgactacc ccagtaactg gagaatagat atgtccgttt tctggttcta
    23281 caaagtagcc ttcacccatc atcttttgtg caaaaactgg atcacttgct ttatctaaag
    23341 aaacaactgt tccgtcagca actgatgaga ggatagtcac actttcagct gaagtttgag
    23401 gtactacttt ttcaggcact tcatttactg tggctgtttc agtcgctaat tcttcttttt
    23461 ctaaacggtc tttaggaata ccgaagaaga atgtagcaac gaagccacca gcataagctg
    23521 ccaataatcc aaggatgtag cctaaccatt taccgtcaga gatcaatggg atcaaagcgg
    23581 ctcctgaagg tccaatagcg atagccccga catttccgat cattccgatc actgccccgc
    23641 cgatacctcc accgatacaa gcagtaataa atgggcgtcc tagaggtaat gttactccgt
    23701 aaatcaatgg ttcgccgatt cctaggatcc cgactggtaa agctcctttg atcatttctg
    23761 ttaattcttt atcttttttg cagcgaatcc ataatgccaa tgctgcaccc acttgtcctg
    23821 caccagccat cgcaagaatt ggaagcaata gcgtacgacc agtttcatca atcatttgca
    23881 catggattgg agtcaaaatt tgatgcagtc cgaacataac catcggcaag aataatgtgc
    23941 ctagaacgaa gccggcaaat gctccaccaa cgccaagtac ccagttgatc ccaccgacca
    24001 tgccatcaga aatgaaacct gctaaaggca tgatgaagaa aatttcaatc agaccgatgg
    24061 caatcaatga aagagttggt gtaacgatga tatctaccgc atcaggtact actttatgca
    24121 gttttttctc aacgattgac aatagccaaa cagcgaagat gacacctagg atcccacctt
    24181 gtccagctgc aagagcttca ccagtaaata agtttttcag tggtgcttcc ggattcatcc
    24241 ctgtcagcat cacaacggcc ccgataactc caccaagagt tggtgttgca ccaaaaactt
    24301 gtgcagcatt gatacctgta tagataacta ggtagctaaa catcccgttt ttcaagatgt
    24361 tcatcacatc gatatattgt tgccatgttg ctgcatcaag tgttccggct gtgactaagt
    24421 ttgctaaaac tgcggcaatc cctgccacga tcccagtacc gacaaatgca ggaatcattg
    24481 gcacaaagat attagaaatg tcttttaata ccgatttgat tttagatggt ttttgtttcg
    24541 atttttgtgc cgctttcatt tcttgtgccc gttcgtttac acggtctttt ccagatgctg
    24601 atgaatttgc tgtagctgta cttacagaag aaagtttttc tcctaatttc acgcctgcca
    24661 aatcaaccat ttgctgtgct actttgttca ctttcccagg tccgatgatg acttgaagct
    24721 gttcatcttc tactacacca agcactcctg gaatcgcttt taattcagag acatttacaa
    24781 gatcatcatt gatgatcttc atacgcacac gagtcatgca gtggacaatg ctttctacat
    24841 tgcccatgcc gcctgcccct ttataaattt gttttgccag ttcttccaaa gtcagttcgt
    24901 tttttgccat ggtcttttcc ccctcataag gtccctttta caaatccgtt tgcttcagct
    24961 aattttgctt gagcttcctc tttatcacta tttgtcaaaa tcatgacgat agccagctta
    25021 acattttgat ctgcttcttc aaagtacttt tcagctgttt cgtaatcagc ttctgtcgct
    25081 tgcatgatga ttcgttttga gcgttcgatt agtttttcat ttgttggttt gacatcgacc
    25141 atcaagttat tgaatacttt acctatacca atcatactga tagtagaaat catattcaag
    25201 ataagttttt gtgctgttcc tgacttcaat cgagtagagc ctgtcaaaaa ttctggacct
    25261 gcatcgactt cgattgggaa atccgcgtgt ttactgattt cggcttgttt attgcaagaa
    25321 atcgttcctg tgacagcacc gatcgatttt gcgtaatcta atccaccgat cacatatggt
    25381 gtacgaccgc tagctgcgat accaataact atatcatttt ttgtcaaatt cagcttgatt
    25441 agatcttctt gccctagctc ataagaatct tcggcccctt cgacagctac agtcattgct
    25501 tcttctccac cagcaattag acctatgacc atttccggtt ctacaccgaa tgtcggtacg
    25561 cattcagcag catctaacac acctagtctt ccacttgttc cggctcccat ataaatcaga
    25621 cgtccacctt ttttaaagga ctcaatcgtg ttagcaatca ctggttcgat acttgtcagt
    25681 tctttttcaa ccgcagcggc cactttctga tcttctttgt tcattagttg aacggcttga
    25741 gaaatactca tctcatctaa tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc
    25801 tctaaattga ttttgctcat ttttatcttc cttcttttct tataaattga tagttcatac
    25861 cagctttgat ttggttgatc aatggtagct cttctttgat aactttagct actacattca
    25921 ctttttcatc cgctggcaaa tcatatttgg ttaattggat ctcacccata tagcgcaaat
    25981 acttttcgtt atctaaagta atagaccctt tcatacgcgt agctgtattt cttgctggga
    26041 taggcggaat ctctcgaaaa cgtgcatcta cgcttcgtat gacatctctt gcatcgtctt
    26101 gccgattcgt atgcttccct aatatatatt cgtaaaattc ttcatctaca ggttccgtat
    26161 ggagcaagac tgtctgttct ttttggaaag aagcaaattg ctcctgaacg ttttctgaca
    26221 gacctgcatc tccgatgtaa acaatatccg tgttacttgc taatagatct agagcgcctg
    26281 caagaggatg gatccctcga tgttcttcca aagttggcaa tccttgatat agtgggcctc
    26341 tcaactctgt atctcctggt acaaaacctt ggacaaggag accttgaatt ttgagccatt
    26401 gatttttttt ctgataccat tctttgtcca gccctgtttc gggacgtgga taataattat
    26461 gccaagcttc aatctgtgag aaatcagctt ctgcttcttt caattcatcg atatctttag
    26521 gcgtgatcgt gctagcattc aaactgattt tcaattgatg tgaccagtcg gcaatctgct
    26581 gattggaaat atgataatcc attcttaaac ctgttacacc aattgctttc aaaggcgtta
    26641 gatcttctaa tgaaaaaccc gctcgttcca atgcctctcc tgaaatatcg accatcaatt
    26701 ctagtttatt ctttttcgct tcgcttccta gttcgatcaa tcgcttttga tatgccgaaa
    26761 catcatcttc tggtatatgc atggaagtga aaataccgac aaagccattg gatgccattt
    26821 ggcggatata ctgtttcttc tcttctgaca aagaatcgtt cataaatatt gaaaagccaa
    26881 acatattatc cctctttttc tgtatccgtc ttcatttcta tgactagagt atatatctaa
    26941 agaaacaaaa tttcaatact tttattaaaa taattaaatt tcatttctaa aggttgagtt
    27001 tatattatca aaagaattgt cataacctaa aaaaggcact gctaagcaaa agcagtgcct
    27061 tgaaatattt tatttaaata acacaaaaac ataattactc ataagaaacc aattctgtat
    27121 tatctaccat gaatgtaaag aacccctcat taggcatcgg atgaaaataa gcattgatca
    27181 ctcttatcaa ggcattatgg caaactaaca gatagacatt ttcttcgtct tcgatgcatt
    27241 ctttcaataa aggaacgatc cgtgcataca catctaagac tgactcccca tttgggaatc
    27301 gtacagcgaa cgctaaacgt gctttttgaa agttctcatc tttgcttggc agtccatcat
    27361 aatccccaaa atccatttct accaatcgtt catcaacggt cagcggcaag ctgagacgat
    27421 cagcaactgc ttgtgctgta tctcttgctc gttgaagagg ggaatggatg attttcgtga
    27481 ttggaacagg caaatctact accttttctg ctagtttctg tgcctgcatc tgtccttttt
    27541 cagtcagcgg cacatctgcc cgtccgcaaa ctaatccttg tgcgttccaa gttgtttctc
    27601 cgtgtctcgt tacataaagc atgtgattct ctccttcgtt ggcaaagttc cccttaacca
    27661 taccttgttg ttcttgagat tacaagggtt agattctctc aagagaaata atctcagtag
    27721 atactttatc caggaaggtt tgatcatgtt ccactagtaa catggcaggt ttcacgttca
    27781 agatcagttg ttctaattgt tcttgattga agacatccaa ataattcaat ggttcatccc
    27841 atgtatatag ttcagctggc tgtgataaag atttagccaa ttccactttt ttccgttggc
    27901 ccatactcat ctgctcgatc ttgttatgaa aaacatctct ttccatccca agctttcgga
    27961 ggttgttcaa aaatgcttgg tagtctactt ggtttttctc tgcaaattcc gccaacgttc
    28021 ctcgattgtc ttcataattt tggcttgcat aactaatgct cagatgtttt ggctggtatt
    28081 tttctcctat aaccttgccg ttgaatgccc ccagaagata atggataatg gatgactttc
    28141 ctgcaccgtt aggacctgaa atcgccaccc gctgatgagg ctcgattgta aaatgaattg
    28201 gctcgaataa cagattttca taccctaatt gaagatcttc tacgcttaaa agtcgcttat
    28261 ggtgagacgc ttggctattc atcgtcaaag aatcgatata ttcgatatct tttaatagtt
    28321 tttctttctc gctgatctgg gtctccatcc gattcacgat tgctttggat cgtttcatcg
    28381 tccgtgcagc atcagcaccc actgctcctt tattcactcg tctagattca gtatcgatga
    28441 atccgacttg tttctttgtt ttatctcctt ctcgggaacg agaccattcg gctttttcag
    28501 ctgctgtttt ctttagccta ctgacttctt ttttcaattt ttcattttga gccatttcaa
    28561 attcatcacg aagttttttc tgttcttcat agatagagaa attcccttga taaagttcca
    28621 gttgactttt ttcgattgct aaaacatggt ccactacttc atcgataaat ccccggtcat
    28681 ggctgaccac gatgaagcct tgttttttct ttttcaaata agccgctact tgttttctac
    28741 cagagatatc caaatgattc gttggttcat cgattaacgg gaaatgagtg tcatccacaa
    28801 ataaaagtgc cagtaggact tttgtcttct ctccccccga tagtgtgctg aattctctcc
    28861 ataagatttc aggatctgtt tgcatcaatt ggagctctct ttcgatttcc catatctcaa
    28921 aatccgtaat atcatttaac acgtaatagg ttaaacgttc tttatctttt gtctgttgcg
    28981 ggaaataggc aaattcttgc tgatggatta cttgcccttg ataaggtagt ttgttttgta
    29041 gaatattcag taaagttgtc tttcctcgac cgtttcgtcc gataagtcct agtttccatt
    29101 gtgtgtcaaa atttagattt gcttgttcaa ataataatgt gccttggctg tcgtagccga
    29161 atgtcagatt ttttatttcg attttagaca ttttccatca cctcgtaaaa aaagaccagt
    29221 cctattttca gaactggcta aagaggatgc aagggttgga tacacacaaa aagacccata
    29281 catcgaaaaa accaagccaa tcccgaaaat ctgcacactg cttccttgtc tccacaaggt
    29341 acagtctgaa tgcagctttc atcaatcgac ttaatttttc aatgcacgga tccctcactt
    29401 tgtcctatat ttgaaattac gatagcataa agaaaatgat tttgtcaact acttcgggag
    29461 gatggtgaaa aatctgctaa gtgtctgtcc taagattttt gttttttcta atgtttgcct
    29521 gtgttccatt gcttcttcta aacctattgg acccaaatga atacttaaga caattgggag
    29581 aaaatcagaa agtccttcta gctcttggtc tctacttccg caaatagcga ttattggttt
    29641 tcgatatttt tttgcaagca ctgctacacc gtatggaact tttccgtgga tcgtttgctg
    29701 atcgatccgc ccttctcctg taatgatgac atctgcttgt tcgatctttt tttccaatcc
    29761 agtgatttgg ctgactaaat cgaaaccaga aaccatcttc gctcccaaca aaaaaagggc
    29821 tccaccaata ccgcctgctg ctccacttcc tggaatatca tctaaatcca cctcccagaa
    29881 atcttttatt tgctgagcaa tctttcctgc ttgtttatct aagaaagcta gcgtcgtatc
    29941 tattcctcct ttttgtcttc caaattgcca agcagctcct tgttgacctg tatatggatt
    30001 ggtaacatct gcagcgataa tcaattttac tttagaaaca agaacgtttc ttcgatcaaa
    30061 atgaagtttt ttagtagata ataaaggatt tccttctgtg tacccttcta aagaagcacc
    30121 caaggattgg agtaatccta gaccaccatc gctacacccg cttcctccta aagtgacaat
    30181 aatttgttct acttcttgaa gcagtgcatc tttaataagt tctcctaagg cataactgga
    30241 agcaagtcgg atagtttgat ccgttggttt atcaagtaga tctaacccca atgcttgtgc
    30301 agattcgatg actgcggtgt tttttccttg ccaagttgtc aataaatagc tgatttttcg
    30361 ctgacggaat aagagatcca tcccatcaat ggtacgccat gttcccctga gcgcgtaata
    30421 gattgcttct gcagtatttt ctccgccatc tgcaatcggc acattgattt ttcttccttt
    30481 acgatcttcc cagccattta gaactgcctg atttagttca aatgaagaag cacttccttt
    30541 aaatgaatca attgccgcca aaatatccat ctgtatccct cactttcttc ttaagtaaga
    30601 cacttttttt catttttttc aaacaaaaca atgctatttt cttccatttt tctatatact
    30661 aaaagggaac tcattgaata aaggagagaa aaaacatgaa aatcgcaata gctggggccg
    30721 gagcaatggg cagccgaatc ggattgatgc tacatcaaag cggaaatgag gtcctcttga
    30781 tcgatcgttg gcctgctcac attgaagcca ttcgtacaaa tgggttgatt gctgacttta
    30841 acggtaaaga agtggtcgca aaactgccaa tctattcacc agaagaaatc atcgaaagca
    30901 acgaacacgc ggacttgatc gtagcattaa caaaagccaa tcaattagat gatatgtttt
    30961 gttcgatcca atccattatt actgacaata cttatgtttt gtgtttacta aatggattag
    31021 gtcatgaaga tgtactggaa aaatatgtgc caaagaaaaa tatcttattt gggatcacga
    31081 tgtggacagc tggtctagct ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg
    31141 aactagagaa cttggaacca gaaggcgaag catttacaaa aaaagttgta gaagtctttc
    31201 aagaagcaaa tttaaatccg atctatagcc ataacgttcg ttattccatt tggcgaaaag
    31261 cttgtgtaaa tggaacattg aatggtttat gcacgatttt ggattgtaat attgctgaac
    31321 taggcgcgca aaaagcggca gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta
    31381 tcgcagcaaa agaaggaatc atactagatc aagaagaagt gtaccaacat attgcttcta
    31441 cttatgatcc tgacaacatc gggttgcatt acccatctat gtaccaagac ctgatcaaaa
    31501 accatcgttt gactgaaatc gactacatca atggtgccat ttggcgaaaa ggacaaaaat
    31561 atgatattgc tacaccatat tgtgcctttt tgacccaatt agtccatgca aaagaaggaa
    31621 tccttggcgc agaataaaag gctctctgat gagaatcagc tgaatcagca gctggttctc
    31681 tttttttaag cccaataaaa gatcaaaaag aaaacacaac tctcatgatg ccataagaat
    31741 tgtgcttgtt tgtttcagta cataatctct tcaaaatgta ggttttttat ttgttttcaa
    31801 cagaggagag gtcatattcg tattgctcat aaatagtgga atcgttagtg ataaatcgtt
    31861 ttgcccaatt ttctgcatca taaaattcca atagctctga taggattggc ttaaatggtg
    31921 tatgcagaaa ttccggtaat tcatctaaat cacgttctcg aagcaaaaaa atattttcag
    31981 gacgataaaa atgataatga gcaatcgctt gattatccgt cactagtttc ttttgaaaat
    32041 acatggattc aaatgtccgc aaagtcactc cttgctgacc ctcccgcaac agttccagaa
    32101 tcccgcgcga ttcccctgtt tttttcaggt actcttcata agtaagatat ccaggttgtt
    32161 cattccctcg atgtggaaga atgaaatagt tggtcgtaac atctcttttt ataaattcct
    32221 gcttatagct ttctgctcgt tcttttctcc cattattcgt tgcaccaaaa aatagatccg
    32281 tctttggttc gctagaagaa aaattcatat gtttcgaata aaatgaacta ttatgtttca
    32341 gcccaagttc ttttgcttcc aaaaaatcaa aatgataaat ctcatcgatt gccggttcat
    32401 cttgtgcctg cttcagcatc gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca
    32461 caatttttgc attggtaaat gggcgcacgc gtcgagccat ctccatatca aatccataat
    32521 ctaaaaaaac gatcagctga taatttgcca actgttgtct agctgattta atgaattccg
    32581 actgtagtaa atcttttccg gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa
    32641 gtgatttatt ttttgcaact agcagtacgt catttttgtt catcttttcc gcctcacttt
    32701 ctctgtcact ttttcctatg atagcctgtt taaatagtaa aaagccagta aaacgtttcc
    32761 tatatttgta tgaaaattta acatttgtat gaaaata
//
LOCUS       DAJSVF010000026        31345 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSVF010000026 DAJSVF010000000
VERSION     DAJSVF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.027"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(54..500)
                     /locus_tag="ON550_001206"
     CDS             complement(54..500)
                     /locus_tag="ON550_001206"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR3954302.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRIRGITVATGRFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(511..2724)
                     /locus_tag="ON550_001207"
     CDS             complement(511..2724)
                     /locus_tag="ON550_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR3954303.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(2957..3721)
                     /locus_tag="ON550_001208"
     CDS             complement(2957..3721)
                     /locus_tag="ON550_001208"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR3954304.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3722..4669)
                     /gene="prmA"
                     /locus_tag="ON550_001209"
     CDS             complement(3722..4669)
                     /gene="prmA"
                     /locus_tag="ON550_001209"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR3954305.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQIVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4682..5170)
                     /locus_tag="ON550_001210"
     CDS             complement(4682..5170)
                     /locus_tag="ON550_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR3954306.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5136..5807)
                     /locus_tag="ON550_001211"
     CDS             complement(5136..5807)
                     /locus_tag="ON550_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR3954307.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5945..7231
                     /locus_tag="ON550_001212"
     CDS             5945..7231
                     /locus_tag="ON550_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR3954308.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7303..7497
                     /gene="ssrS"
                     /locus_tag="ON550_001213"
     ncRNA           7303..7497
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ON550_001213"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7575..7859
                     /locus_tag="ON550_001214"
     CDS             7575..7859
                     /locus_tag="ON550_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954309.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7932..8411
                     /locus_tag="ON550_001215"
     CDS             7932..8411
                     /locus_tag="ON550_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3954310.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8634..8777
                     /locus_tag="ON550_001216"
     CDS             8634..8777
                     /locus_tag="ON550_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR3954311.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9355..10938
                     /locus_tag="ON550_001217"
     CDS             9355..10938
                     /locus_tag="ON550_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3954312.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNPYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDESTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11375..12559)
                     /locus_tag="ON550_001218"
     CDS             complement(11375..12559)
                     /locus_tag="ON550_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR3954313.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12584..13591)
                     /locus_tag="ON550_001219"
     CDS             complement(12584..13591)
                     /locus_tag="ON550_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3954314.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13763..14122)
                     /locus_tag="ON550_001220"
     CDS             complement(13763..14122)
                     /locus_tag="ON550_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954315.1"
                     /translation="MPYGQKPISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14094..14465)
                     /locus_tag="ON550_001221"
     CDS             complement(14094..14465)
                     /locus_tag="ON550_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR3954316.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKTDQ"
     gene            complement(14482..14784)
                     /locus_tag="ON550_001222"
     CDS             complement(14482..14784)
                     /locus_tag="ON550_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954317.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(14801..15271)
                     /locus_tag="ON550_001223"
     CDS             complement(14801..15271)
                     /locus_tag="ON550_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR3954318.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15246..15524)
                     /locus_tag="ON550_001224"
     CDS             complement(15246..15524)
                     /locus_tag="ON550_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR3954319.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15521..16570)
                     /locus_tag="ON550_001225"
     CDS             complement(15521..16570)
                     /locus_tag="ON550_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR3954320.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16527..17456)
                     /gene="tadA"
                     /locus_tag="ON550_001226"
     CDS             complement(16527..17456)
                     /gene="tadA"
                     /locus_tag="ON550_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR3954321.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17588..19342
                     /gene="ade"
                     /locus_tag="ON550_001227"
     CDS             17588..19342
                     /gene="ade"
                     /locus_tag="ON550_001227"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR3954322.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19440..20102)
                     /gene="trmB"
                     /locus_tag="ON550_001228"
     CDS             complement(19440..20102)
                     /gene="trmB"
                     /locus_tag="ON550_001228"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR3954323.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20150..20932)
                     /locus_tag="ON550_001229"
     CDS             complement(20150..20932)
                     /locus_tag="ON550_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR3954324.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21136..22347)
                     /locus_tag="ON550_001230"
     CDS             complement(21136..22347)
                     /locus_tag="ON550_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954325.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22340..23077)
                     /locus_tag="ON550_001231"
     CDS             complement(22340..23077)
                     /locus_tag="ON550_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954326.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDTAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23215..23649
                     /locus_tag="ON550_001232"
     CDS             23215..23649
                     /locus_tag="ON550_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR3954327.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23642..23986
                     /locus_tag="ON550_001233"
     CDS             23642..23986
                     /locus_tag="ON550_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3954328.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            24166..25176
                     /locus_tag="ON550_001234"
     CDS             24166..25176
                     /locus_tag="ON550_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3954329.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLKSSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(25245..26189)
                     /locus_tag="ON550_001235"
     CDS             complement(25245..26189)
                     /locus_tag="ON550_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3954330.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(26173..28893)
                     /locus_tag="ON550_001236"
     CDS             complement(26173..28893)
                     /locus_tag="ON550_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3954331.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(28890..30110)
                     /locus_tag="ON550_001237"
     CDS             complement(28890..30110)
                     /locus_tag="ON550_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR3954332.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKIPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(30391..31236)
                     /locus_tag="ON550_001238"
     CDS             complement(30391..31236)
                     /locus_tag="ON550_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3954333.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         9316 a         6447 c         5452 g        10130 t
ORIGIN      
        1 gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt actttatttg
       61 tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac cgacatatct
      121 gctccgaatc gtccggtagc gactgtgatg ccgcgaattc tgattccttc attgaatgat
      181 tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg tcgatttcct
      241 tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc tcccacatct
      301 tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg tgcaattttt
      361 tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa taaaacaagt
      421 aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc tttacttacc
      481 cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta cactgtagac
      541 atcaggtact gctttgattt tatccacgat actctttagg tgggataaat tctggatacc
      601 aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa cgctgttgag
      661 atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg aacgattata
      721 gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat cttcccattc
      781 gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg gacaatctcg
      841 acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat caccaggaat
      901 cggattacag cagcgactga tacgaattag taagttttct accccttgaa tgacaacgcc
      961 accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca tgatttcttc
     1021 tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag tcagacgatt
     1081 ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat catcttctgt
     1141 ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa tatctttagg
     1201 cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga tcacattttc
     1261 ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc tagtagcaac
     1321 taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga tctcaatgat
     1381 atcgccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt ttgcaccagt
     1441 ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc cggatccttt
     1501 tggtaactca gtaacgtctc cctttggtgt gaagacgtag actttatcac taaagatatc
     1561 tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa gttcgagtat
     1621 ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt cattttttcc
     1681 ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca tttcctgtgt
     1741 tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat gcaaagattg
     1801 gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca ttggtttcca
     1861 ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta cgatcactcg
     1921 aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca ttttacgata
     1981 gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca gctcttctgt
     2041 tgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt ctctcttcgt
     2101 ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa gtgctgtatc
     2161 ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg catagatttc
     2221 taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca acgtacgcat
     2281 gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag ccattgctag
     2341 cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt ttattttccc
     2401 taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt ctctcagatc
     2461 ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag ccactgtatg
     2521 gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa taatgtaggg
     2581 ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc atgctttctc
     2641 aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga caccgggtcc
     2701 cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc ggtaaaacgg
     2761 gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta tacgtttttt
     2821 tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc attgtataaa
     2881 taattgtttc tattatacaa attgttgact attttttcaa ttctttccgt cgatactctt
     2941 ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga tagctagcag
     3001 cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct aatccacaaa
     3061 gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca ccttctggtc
     3121 caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg gctgcatttg
     3181 atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca taatcatcta
     3241 gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg atctgccgat
     3301 gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt ttcatacgtt
     3361 tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa gcttgcgctc
     3421 ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga tagccactcg
     3481 ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt tctttcagat
     3541 aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga tcattgaatg
     3601 ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg tgatagtttt
     3661 ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa taccgctgca
     3721 tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc tggaaaattt
     3781 gatccacgat aaacccttgt tctaacatgg catccagaac tatttgcttc ttctcttcaa
     3841 tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca tctgggatca
     3901 tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca atctcgattc
     3961 ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc atatccatat
     4021 tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca tcttttgcac
     4081 caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct agcaatgttt
     4141 caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg ggatgagtgc
     4201 ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga tcttctgtct
     4261 ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc ggatgatagt
     4321 atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt actttatttt
     4381 ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata aaaggcaaga
     4441 tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct tctttcagcg
     4501 atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa tgttcaacat
     4561 ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata ttagcaactg
     4621 cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata tctgtcactc
     4681 cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt acttctacta
     4741 attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca tcgtcttcat
     4801 ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga tattccacta
     4861 aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt actgctagat
     4921 aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg tcttcaaatt
     4981 cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat tgtgctttat
     5041 tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc caatctaaag
     5101 ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt aatagatcct
     5161 tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt tctttgtaaa
     5221 gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca tacacctttg
     5281 tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt tttctcaaaa
     5341 actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg aattgctaat
     5401 gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc gccacgattt
     5461 ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac tccttgagga
     5521 atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt gtaaaggtag
     5581 atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa accatagcta
     5641 tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata tccagctaat
     5701 cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc cacttctggc
     5761 gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt tctcacctac
     5821 tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga aggctgttct
     5881 ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga aggaggaaaa
     5941 tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg aagtcgttgg
     6001 tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg ctcgcatgct
     6061 ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg caagtgcgat
     6121 cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt caaaaaagga
     6181 tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct tattggatga
     6241 agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag aaagtggccg
     6301 aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc cagcgatccg
     6361 cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc agttggcagt
     6421 cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc agttagacga
     6481 agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg cattgaatgg
     6541 gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc atcttacact
     6601 gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa atggagatgc
     6661 tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg tggatgctgc
     6721 acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt gccggcgtct
     6781 gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg ctcgtacagt
     6841 gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc cactagcaga
     6901 cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg cattagatgc
     6961 agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt tgagagatag
     7021 ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc ctcatcattt
     7081 tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg ctcagtatta
     7141 tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt accgtataaa
     7201 agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt tctgcgagct
     7261 ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct gtgatgttcg
     7321 aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac ccgtcttgtg
     7381 tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat gggacccacc
     7441 tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg agattaccat
     7501 cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg ttgcttattt
     7561 tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta ttattattcg
     7621 ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac aaaaatgata
     7681 aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc gtaatgggta
     7741 ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg atcgtaatta
     7801 ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca aagcagtaaa
     7861 taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat tgacttgagg
     7921 agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat ggttctgatg
     7981 aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac ggagaacttc
     8041 tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt gatggaatgc
     8101 ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa agcaatgcga
     8161 agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca aaacaaatta
     8221 ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga gccacaggat
     8281 tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt agaaacgcag
     8341 cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct aaagaccaca
     8401 ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc acaaaaaacc
     8461 gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt aaaataaagg
     8521 gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc gattagtcgc
     8581 cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc aatgtggaag
     8641 tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc gattttatcg
     8701 ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct acattcggta
     8761 aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac gatactcctg
     8821 aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg tattaagcaa
     8881 atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga tttcgcctga
     8941 acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct cagtcagata
     9001 aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc agcgctagtt
     9061 gaactttctg acactttcag gtacactgga aaagacaact acacgaagga gggaaaatac
     9121 tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc atatcgcatt
     9181 tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca aaacagttga
     9241 attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct gagtttaaat
     9301 aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga gatcatgatc
     9361 atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga ggatttatcc
     9421 tgtcaaattg aagctggtga aaaaattgcc ttagtcggta tcaacggaac tggcaaatca
     9481 acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat cagtcgcaaa
     9541 aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc gaccgtcaac
     9601 ccctacttgc tccattctgc cgctgagata caggacctca aacagcaaat gagtcgctac
     9661 gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg ttacggtgag
     9721 ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat tgcagcgatc
     9781 ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact tagcggaggc
     9841 gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt acttttactc
     9901 gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa ctatttacag
     9961 aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga tcaagtcacc
    10021 acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa ctacagccgt
    10081 tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga actgcagcaa
    10141 aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc acatgaagga
    10201 gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc gaaactgaca
    10261 ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa tggtggaaaa
    10321 tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga tcaagtacta
    10381 ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat cggcgacaat
    10441 ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc ttacggtggc
    10501 accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct gcagtttgcc
    10561 gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga agaagtcgct
    10621 cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg gatcaaagat
    10681 ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga aaaaatcaac
    10741 gtcctgattt tggatgaatc aaccaatcac ctcgacagct atgctcgaga agaaattgaa
    10801 gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg ctactttttg
    10861 cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg ccagccgctc
    10921 ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc atacctgtgc
    10981 tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg atcaatgaat
    11041 gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca gcccacgagg
    11101 ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag atgaatgaaa
    11161 ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt tcccttttga
    11221 ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga aattgaagtt
    11281 aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc cctcttgcat
    11341 gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc tccacatcac
    11401 gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt gattcttttg
    11461 tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt tcacaaccaa
    11521 accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg ccgataccag
    11581 cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg atgtattttc
    11641 ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca tagttctttt
    11701 caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat ttcttgttca
    11761 aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat gggagaacag
    11821 ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt gtgaatccca
    11881 tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca tttcctaagt
    11941 ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc gcacgttccg
    12001 acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa tcttcataat
    12061 aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg aaagatgtat
    12121 cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg atccccatta
    12181 agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg tccattactt
    12241 tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca cggtgcccta
    12301 ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga tctaacagca
    12361 tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt tgattatcgc
    12421 cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc cctttcgcaa
    12481 ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc ccagcattga
    12541 ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga gcagatgatt
    12601 tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt aatgaagcaa
    12661 gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc tgtaagatca
    12721 aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga atcattcctt
    12781 gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc gtttctaaaa
    12841 atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc attgattgct
    12901 ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa aactcttttg
    12961 ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa tcgacaggat
    13021 ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct tgtgtcaaat
    13081 cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca attccttctg
    13141 tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg cccattcctg
    13201 ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt tcaataagaa
    13261 aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc aaatgttctg
    13321 tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct ggctcgaaag
    13381 aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct tttgtcggca
    13441 ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt tcgacatagg
    13501 cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc aggttttgat
    13561 taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga tatattataa
    13621 caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc gttttcactg
    13681 ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata acagaaaaga
    13741 gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt tttacttctt
    13801 ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac tgttgtaccc
    13861 actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt tcggcaagcg
    13921 tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa tttttgattc
    13981 gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat aaaaacgtac
    14041 ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat tttctactga
    14101 tcggtttttg tccatatggc atatccatct tctccatttt caaagtgtac ataaaaagaa
    14161 attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat aggttgatgt
    14221 ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat atactgttta
    14281 tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact gctcttgagt
    14341 tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg ctgcaaaaat
    14401 tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag actgctcaat
    14461 atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg ttttttcaat
    14521 actgatcgct tctttctcca tattgacaaa cactttttcg attactccat tattatcgac
    14581 aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt gatacatccg
    14641 ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc cacttttctt
    14701 ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga cagaagcgat
    14761 cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt ctttttgtat
    14821 agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct tcccaataaa
    14881 attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta atagaagcat
    14941 ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt ttatctggat
    15001 tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct gtttggatct
    15061 gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg ccgaaaaatt
    15121 gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa gaaaggatcg
    15181 ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat gctaaaacct
    15241 taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc ttcattcaac
    15301 aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc tttttgtgtg
    15361 tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct ataacgcgac
    15421 aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat tagcatttcg
    15481 atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa acctcctcta
    15541 tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa gccgctacgc
    15601 ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg aaaagctgtt
    15661 ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt gcctctccct
    15721 gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac tgctcagcca
    15781 atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga atcagacttt
    15841 cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct tcatgaaaaa
    15901 gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc ggtccaatca
    15961 ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc agacgatacc
    16021 ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac caatgaaaga
    16081 cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca ataagctgag
    16141 gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc aatagcatcg
    16201 gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga aactgtttgg
    16261 caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg atctgtacca
    16321 gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc ccttccttta
    16381 gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg agtaataaat
    16441 aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt tgtgcaaact
    16501 ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat attttcctga
    16561 gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat aaattgataa
    16621 ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata aatgacacca
    16681 gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct gaccaatgta
    16741 ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag tgctgctctt
    16801 accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa gtctggacga
    16861 tgccgtaatg cgagtttcaa aagtgtatca taagtctgtg ctattttcgg attgatctgt
    16921 agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat cacttgcaat
    16981 ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac tggtccgctg
    17041 aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc tcgagaacta
    17101 aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa actctctcgt
    17161 tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc taaagaataa
    17221 gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc caaatactta
    17281 aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa tgttcgtttt
    17341 tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata tacgtcttgg
    17401 gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat ttccattatt
    17461 ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa aatgaattta
    17521 caaaaaaata ttggcaaaag ccgaatttat gttatactta taaaaattta tcttgggagg
    17581 cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa aaaagcctgc
    17641 tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa tcatggaaaa
    17701 agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag gaaaaagcgt
    17761 acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg tgcatatcga
    17821 aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg gagttactac
    17881 tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc ttgagtttat
    17941 gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt cttgtgtacc
    18001 tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag cccctttttt
    18061 acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag tagcaaataa
    18121 tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa aaattgacgg
    18181 gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag gtattcggac
    18241 ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg gtatgtattt
    18301 aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg cgatcacacc
    18361 tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg atttagtaaa
    18421 agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg atccattaca
    18481 agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa cactaggtgc
    18541 gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca agctgccaat
    18601 tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc aagaccattt
    18661 atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg ctcaaccatt
    18721 aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga tcgaaattat
    18781 cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag gcgaacgatt
    18841 cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc accatcaaac
    18901 gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag cgatagcgac
    18961 aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg aggatatgct
    19021 gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca acaaacagca
    19081 ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac ctttttcgat
    19141 cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag cagctaaaac
    19201 ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg aattgaagat
    19261 caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt gtggaaaaac
    19321 cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac atctgtaatg
    19381 ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga aagttagttt
    19441 cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata aatacgttga
    19501 ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa ttgactttga
    19561 tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact tgccagagaa
    19621 tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg acgaagaatc
    19681 tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg acgttttttt
    19741 ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc gaaatactct
    19801 gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg ttcgatcaac
    19861 ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata attgatttca
    19921 ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat ctcaatgtgg
    19981 attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt tggatcacta
    20041 attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt tcggacacgc
    20101 attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaacct cagccactga
    20161 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
    20221 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
    20281 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
    20341 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
    20401 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
    20461 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
    20521 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
    20581 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
    20641 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
    20701 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
    20761 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
    20821 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
    20881 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    20941 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    21001 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    21061 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    21121 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    21181 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    21241 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    21301 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    21361 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    21421 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    21481 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    21541 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    21601 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    21661 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    21721 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    21781 tgcccatatt agccaataaa atgatagaaa taaaaagatc agagaagaaa aaaaccagcc
    21841 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    21901 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    21961 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    22021 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    22081 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    22141 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    22201 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    22261 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    22321 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    22381 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    22441 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    22501 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    22561 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    22621 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    22681 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    22741 ttttcattgc tgtatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    22801 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    22861 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    22921 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    22981 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    23041 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    23101 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    23161 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    23221 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    23281 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    23341 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    23401 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    23461 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    23521 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    23581 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    23641 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    23701 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    23761 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    23821 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    23881 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    23941 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    24001 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    24061 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    24121 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    24181 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    24241 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    24301 gaagcaaaac ttaaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    24361 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    24421 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    24481 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    24541 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    24601 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    24661 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    24721 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    24781 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    24841 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    24901 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    24961 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    25021 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    25081 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    25141 tcttcaactg aagaaacaac tgattcatca aaataaaaga aaacaaaaaa agcgaagact
    25201 tatgtctcgc tttttttgtt ttgaatgaaa aaaataaatt tttgctataa atcttttggt
    25261 acatagaaac ttcggttgtc cataccgaaa atccgatcgg tgtattttcc tggttctgtt
    25321 tgacgaatcg aagtcaacat catctgcatc gaagcatcga tattgtcgat ttgatgtaat
    25381 atttctgctt ccattattct tggacgtaca ggtgagccat attctaataa gccatggtgt
    25441 gctaaaacca tatgacgtaa tacgagaaca tcttcgtcag cttcgctgat tttcaacgca
    25501 agacaggctt tagttatttc ttcgtcaatc aataccagat gaccgatcaa atttcctgca
    25561 agcgtatatt ctgtcgacat aggaccggaa agttcaatca cctttcctaa atcatgcaaa
    25621 atgataccag catacaacaa tgctcgattc agctcaggat attcatcggc gattgatttc
    25681 cctaagtgaa gcattgtcaa agtatgataa gctaatccac tggcaaatgc atgatggttt
    25741 ctttttgctg ccggaaactc aaaaaattgt ttttgatatt tattaagtag atagcgaaca
    25801 atacgattcc aatgcgcatt ggtgatctca aacaacaatt gattgatttc ttcttccatt
    25861 tcttctttct tcaaaggggc tctttccatg tataaactag gctctcctgg ctcatctacc
    25921 cttgttaagc gcatatgaag aatctttact tgagggtttc cttgataaac ctctctttta
    25981 ccgtttagaa aaacgactgt gccggctgta aatttattga tttcctcctc cgaagcatcc
    26041 cagtatttac catcgatagt acctgaagta tcttggaaag taaaagcaat aaattttttt
    26101 ccgtttttag cgattcttac atctgcactt ttgatcagga caaattgttc aaactgctca
    26161 tcgattacta aatcacgaat ttttttcatt atttgctcct tctaattgtt ttacgatttg
    26221 gttcatctca tggtaatagc ttaccatttc tttatcagaa gaaaaacaaa ttatttgata
    26281 cttttctcca aatttagcta aaagttcagc taattgatac tttcgctgat gatcataatg
    26341 aagccagcca tcatcaatga tgatcgggca tatcgaacgg ttagcttcta atgagaggta
    26401 agcaaatctc atcgccataa tcagctgatc tttcgtacct gtagataact catagatggg
    26461 aaattctgta tatccgtctg ttaaagtcag tgtatcattt ttcagtaata tcttctgata
    26521 atttcctttt gttaaaatac ctaaataatc acctgctgct cttaacaact ggggtaattg
    26581 tctttcactc atctctgttg aaagatcacc taagaatgtc ccggcgattt caagtgctcc
    26641 ccattcctcc agtaattcct gtaattttgc tttttgccgg ctagcttgtt gataaagttc
    26701 gtccaatgtg ccatctcttt gcaactcttc tacttgaagt tggagtcgtt gccgattttc
    26761 acttgcttct ttttcttctt gctggagttg ggattgcttt tctttcagtt ggcgcagtct
    26821 tacagataat ttctcttttg tcagctcttc aggaaatagt ggagtaagca tttccgataa
    26881 ttcattgtat cttttcatct gctgttcggc ttgtttggct ttttgtaaaa agacaggtac
    26941 ttccgaagga taagataagc ctgcttgagt caattgttcg ctgtattctt gtatccatac
    27001 tttttgtttc tttttcaatt gattgatttc ttgcttcaat aaagtatttt gctgatagct
    27061 tctggcaaat ttcatctgtt ccatctcttg tataaaacgg gaaagcaata ccattttttc
    27121 tgaaattgtc ttatcttgta tcggtaacca ttctttaaca aactcaaatt tttggtcgat
    27181 ttgtcgtagc ttttcttgta tttcctgttc tcttttatgt ttccgctcaa atacgccata
    27241 taatttctga taatctatga cttcctgtcc atattctttc atgagtttga gcgtatccat
    27301 ctttcctaag tgccgatgat ccgcaaaacg atatacttct tgctgaagaa tctgtttttc
    27361 ttctccagtt ctcttttgtt tttcttctgc ttcagctaat tgagctaagg ctagatcaag
    27421 ttgtcctagt ttttcttgcc acattccttt tatctcagag aaatcatttt tagttttaat
    27481 ttttggttgc cttttgtaga agaaatagcc aatcatgcct gcaccaatta gtaaaaggat
    27541 cactttcaag ggcattggta caaatatacc tgcaatgatt attgctccgc cagccatcag
    27601 catatacggt ttagatgaag agactggtgc tatctctgac tgattttgta ataatgctgg
    27661 ataggtctct tccaagtcat tcacttctgc ttcaatcatc tcttgctgtt ctttagctat
    27721 ttggagacgc acagaaatct ggctttctag atcagcaatc cttctttctt cagccagaag
    27781 atgcttccat tcttcttcat ataaaaattc ttccggtaac ggatttttct gccaatgcca
    27841 cctttgttcc aaaatcttca attctgaatt tgtttctcta gtctcaattt gtaatctttg
    27901 ccattcttcg tttaattgtt ccgctgtaaa ttgaagttgc tggagatctt ttagttcctg
    27961 ctcctgctct aaataaaaat aatatctggc tgactgctga tccatcccgc tatgcttttc
    28021 cagtgttgct tctaattctt ccaaacgctc atttgtttgc tgataccgtt tggagaaaga
    28081 ctccagatct aaaagaagtt gctcatcagg aagacctgta gtgctgtttt ttttgagaga
    28141 ttgccattct tcatataaag aaaaattaag ttcttgctgt ctggctaatg aaaattgttt
    28201 gttcgcctct tttaattgtg ccaataactg ctgctgttgc gactcgtatt cctgttcttg
    28261 ttggatcaac ttgccaaaat aagcttcctg ttcttgtttt tgatgaattt tttcttgcag
    28321 ctctttccat tcatttaatt tttgattgat tagtagtttt tggccttttt tcttgaataa
    28381 ttgttgtgct ttctgataat attcttgccg cttttggaat acttgagaac tgcccgtgat
    28441 gcctaaagaa atcagcgcat catgtagctc tttttctctc aaatgatcta gttccatcaa
    28501 ctgctcttgc tggaaagtaa atacctgctg gaataactct ttcgtcaaag gtgcgatcag
    28561 tttttctagt aactcatcac ttcccacttg atcccccagc cacaccttcg attttccgcg
    28621 attttgctgc ttgtagcgct cgacagcaaa ttcaccatat acaggatgtt taagccatat
    28681 tttgccgcca taggctgagc cgtcttttgg tgtataatcc ttttttttac ggccttttga
    28741 agggaaacca aataatattg cttgaataaa ttgataaatc gttgattttc ctgcttcgtt
    28801 tcccccatag atcagctgat tgcctgaata aaagtcaaaa cttttttgac gccattttcc
    28861 aaaagcagca atctcgatcc ttaaaatctt cattcctgat cctcctcaaa taaaaaatct
    28921 tgattaagca gctgcttggc tttttcaagt gtttcttctt gatattccgg cagttcgctc
    28981 aatattctag ccgcttcttg atgactatat acttcattta aaatttgttg gaaatcttga
    29041 ggtgtctgat agtattgaaa taactgttcc atcaatttag cagaagcagc aagtgggatc
    29101 ttcgtataaa aagtattctc tatgagagaa atacgccaaa taaagaaatc atttatccta
    29161 tctgagaatt cctctaacaa atagtctaat agctctcctg attgtatccg ttccaaaaca
    29221 tctgtattga gatggtcata atcttttaat tttatctcaa ttaaactaaa tccatcctct
    29281 acttttgaaa gctgctgacg aatgtgaaca atgacctctt ttgtcgttct tgctttcctc
    29341 aatgatattt cctttgtctt ccaatagacc tctgctactt taatcggatg tatttggcaa
    29401 cttcctttac ttaattcaac tagtagaata gatgtagaag cttgttcctt tttagtatga
    29461 ccttgaggcg cacctggata aacaattgtt tgcttttcat ttaagaccgt tggcacatgg
    29521 atatgcccta aagcccaata atcgtatccc ttttcttgca tctttgaggg ttggaaagga
    29581 gcatagtttc ctttttgaga gacgccaggt tccccgtggt acaacccaat atggtaatct
    29641 gtgagttctc tagaaggaaa ttccatcact ttgtcttttt gaatccacgg ttgacgataa
    29701 ctaaatccac tgagagagac agtttctcca ctttttatcg ttattttttt tgtctctacc
    29761 gtttcggatg taaataaatg gacattttta ggaaaggaga accaataccg ctctttttga
    29821 tagaaatcat gatttccgaa tattataaat acaggtatat ggttcttttc taaccgtttc
    29881 atctgttctg aaaaatgttt ctgtattttt aatgaaggac gattttgatg aaaattatct
    29941 cctgcaagta aaacaaaatc aacgttattt attattgcct gttcaatgat atttgataaa
    30001 acggtcagat tcgtcttcaa tagtttttcc tgtacagttt tatcaagagt tgtcagtcct
    30061 tcaaatgacc gatccatatg taaatctgcc gcatggataa atttcaccat taaaaatttc
    30121 ctcctttttt tcttttatca taccattttc aatgtttttt ttcatcaata accgaaaatt
    30181 tgttcgtatt atgcaaagaa attacgataa atggccgact tcaaaaaata agtttattcg
    30241 aaaaaaagat tgtgacatga tttttgtcac aatcctaaat atctatgtta tattcgcttc
    30301 gtttgacact gaagaagtac tatttgtact ggaatttatg ctccattttt ccgtatttag
    30361 aattctatgt tctatactac cttaattagt ttatatggta agaattcttt caaatgcttg
    30421 agatactccg tctttatcat ttgaaacagt gacataatca gcttttttca tgatttcttg
    30481 atgactattt cccattgcaa aactaatatc tgcactttca aacatagata agtcatttaa
    30541 actatctccg atcgcgattc tcaaaggttt ctttaaattg tagattgatt ccaaacgatc
    30601 aaacaccaca cctttagata cattttgagg tacgatttca atatttacag gtgccgattc
    30661 agtaacagac acatttagca tctttttcaa atggttaaat atattagctt tcaattgacg
    30721 attaggaata aaaataaaaa atttgtaaat gctgatagca tccttttcta taatttcctc
    30781 aatattatta taagggattg aacaaataga tttttcatac tcaagctcat agtttagctc
    30841 tttgatagaa aattcactcc ctacctcgtg aaaaattttt ttcattttat ttttataatt
    30901 ctgagattca taacttccga gatttgtata tattttggtt ggaaatttcg ataggacaga
    30961 atcaattttt tttacatcat ttttatcgaa ggaagactca aatatctttt tttctccatc
    31021 aaatccaaca gaaccattta accctactgc tggtatcctt aaaccagatt ttttgagaat
    31081 attattaatg tcttctactt ctcttccaga acaaatgaat ggaaaacagt cttccggtaa
    31141 atgtttcatt gttcttgcat ttttttgact aatgctctgg tcagaattca aaagagtacc
    31201 gtctaaatct ataccaatta gtttcatttc atccatgtct cctatacgtt gattataaat
    31261 tcactgctta ttgtaagtct aaatttctca caaaacatat actaattata cctaatttaa
    31321 gtaagtacaa acatcatttt aagaa
//
LOCUS       DAJSVF010000027        30364 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSVF010000027 DAJSVF010000000
VERSION     DAJSVF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.028"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(338..1291)
                     /locus_tag="ON550_001239"
     CDS             complement(338..1291)
                     /locus_tag="ON550_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3954334.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="ON550_001240"
     CDS             complement(1314..2069)
                     /locus_tag="ON550_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3954335.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="ON550_001241"
     CDS             complement(2066..3025)
                     /locus_tag="ON550_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3954336.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTFLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="ON550_001242"
     CDS             complement(3018..3968)
                     /locus_tag="ON550_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3954337.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="ON550_001243"
     CDS             4118..4570
                     /locus_tag="ON550_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR3954338.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            complement(4658..6925)
                     /gene="gshAB"
                     /locus_tag="ON550_001244"
     CDS             complement(4658..6925)
                     /gene="gshAB"
                     /locus_tag="ON550_001244"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR3954339.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7112..8110)
                     /gene="ftsY"
                     /locus_tag="ON550_001245"
     CDS             complement(7112..8110)
                     /gene="ftsY"
                     /locus_tag="ON550_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR3954340.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8122..8931)
                     /locus_tag="ON550_001246"
     CDS             complement(8122..8931)
                     /locus_tag="ON550_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3954341.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8940..12521)
                     /gene="smc"
                     /locus_tag="ON550_001247"
     CDS             complement(8940..12521)
                     /gene="smc"
                     /locus_tag="ON550_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR3954342.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPNQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12541..13227)
                     /locus_tag="ON550_001248"
     CDS             complement(12541..13227)
                     /locus_tag="ON550_001248"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR3954343.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13575..15356)
                     /locus_tag="ON550_001249"
     CDS             complement(13575..15356)
                     /locus_tag="ON550_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3954344.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15382..16296)
                     /locus_tag="ON550_001250"
     CDS             complement(15382..16296)
                     /locus_tag="ON550_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954345.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16323..17285)
                     /locus_tag="ON550_001251"
     CDS             complement(16323..17285)
                     /locus_tag="ON550_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954346.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17285..18229)
                     /locus_tag="ON550_001252"
     CDS             complement(17285..18229)
                     /locus_tag="ON550_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954347.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18230..19234)
                     /locus_tag="ON550_001253"
     CDS             complement(18230..19234)
                     /locus_tag="ON550_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954348.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(19502..19744)
                     /gene="acpP"
                     /locus_tag="ON550_001254"
     CDS             complement(19502..19744)
                     /gene="acpP"
                     /locus_tag="ON550_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3954349.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(19866..20867)
                     /gene="plsX"
                     /locus_tag="ON550_001255"
     CDS             complement(19866..20867)
                     /gene="plsX"
                     /locus_tag="ON550_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR3954350.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(20916..22952)
                     /gene="recG"
                     /locus_tag="ON550_001256"
     CDS             complement(20916..22952)
                     /gene="recG"
                     /locus_tag="ON550_001256"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR3954351.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23069..24751)
                     /locus_tag="ON550_001257"
     CDS             complement(23069..24751)
                     /locus_tag="ON550_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR3954352.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(24803..25165)
                     /locus_tag="ON550_001258"
     CDS             complement(24803..25165)
                     /locus_tag="ON550_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3954353.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            25435..25623
                     /locus_tag="ON550_001259"
     CDS             25435..25623
                     /locus_tag="ON550_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR3954354.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            25861..28530
                     /locus_tag="ON550_001260"
     CDS             25861..28530
                     /locus_tag="ON550_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR3954355.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(28621..29241)
                     /locus_tag="ON550_001261"
     CDS             complement(28621..29241)
                     /locus_tag="ON550_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3954356.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            29354..29893
                     /locus_tag="ON550_001262"
     CDS             29354..29893
                     /locus_tag="ON550_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954357.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            29890..30228
                     /locus_tag="ON550_001263"
     CDS             29890..30228
                     /locus_tag="ON550_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HCR3954358.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         8457 a         6444 c         5403 g        10060 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggaa cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa aacgaaaaaa cacataaatt tttacatgca aaaaagcttt tgatcagatt
     4621 gacatcaaat aggacaatct gtcaaaagct ttttctttta aaatacttca ggatataaca
     4681 atttcaatac attcatcgtc aatcttctgc cttttcctgc aaagggataa acatgcatat
     4741 gcatggctgg attgaaattt gcttcgatga tcccatatgc ttttttatca gttgtaggat
     4801 cgatttcttt atctggtata atcaaatcga taccactaat cttagctcct agtgcttcta
     4861 ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc atcagtcata tcgatcgaat
     4921 caccacctgt actgatattt gagttctccc ttagataaac aatttgattg gcttggggaa
     4981 ctgattcgat cgtcaaccct tgttctttca acatcaattg ttctaattca cctaattgga
     5041 tcaattctaa aggtgcacga tgattcgttc cacgcaaagg gtcactattt ttttcttcaa
     5101 caagttcttt cactgttcgg ataccgtcac caataacatt agcagggact cgcaacatga
     5161 tggcttggac ttctccgtcg atcacaaaga aacgatattc cgttcccggc ataaattctt
     5221 ctactaaaac cgaactgtct tcacgaaaag caatggctaa acctgcttga taatcttcca
     5281 gactagctcc ttccttgaaa atcgtgatac ctaacccgta attggttgat ttaggtttga
     5341 tcacaaatgc ctgctcggca aatctcggat aagcttttac cgcttcttcc atggatgaaa
     5401 attctgcgcc tcctggtacc cggaacccag cttcttttaa gacttttttc gttactgttt
     5461 tgttttccat gataagtggg acgatatagc tgtccttact tgtcatgttt gcatttttca
     5521 catattctac atggtcttgg aattgcagac gcaaaaattg atccgactca tctaatactt
     5581 tgacttggac acctttttgg atggcatcga acataaatat ctgagtagaa agttccattt
     5641 cgcgaaaacc ggccaactga tagggacgtt catggctttc tttataatat tgatgtccca
     5701 atgccacagc aacttcttgc tgactcgata gctgtgcttc ttgatagagt ttcgctgcta
     5761 acgtttgttc aggatgatcc atttgagtca atgcttcctc tactaaataa agagaatcca
     5821 accaatccaa ttcaaccagc atttgcttca tcccttcaag aatttctcta ccttcttgat
     5881 gaaattctgt ttgatctgat ggttgttcta gtgctacttt gttatttcga gtcgtacctt
     5941 ctgctaccca ttggtcacag tcttcttttt cttctgtcca taacatgtac atcaggaata
     6001 attgtaagaa ccgaacttgc tctggattga tacccaatct tgcataagga ttcaaatcga
     6061 tattccgcaa ttcaatataa cgaacacctg ttgttgctaa atctgcgact ttctttccgc
     6121 ctcggaaacg aagcggtgta taaaattctt tctcctccga aagtttccct tcttcgatca
     6181 ttcgctcgat atctgctaaa tactgtttca tagaagcata ggaaactttc acatttggat
     6241 gattcgtata gcccagcgca ctgttacgaa tactgcgaac aggttcttgc ggatgtgatt
     6301 catctaaaaa gtagttcttc tcactcattg gtgaagcacc gaataaataa gtgatcatcc
     6361 agcgatagcg cataaagttt ctcgccgttt tcaaataaag ttctgttttg aattcggaaa
     6421 aatcttgaaa ttcttcctga tggctgaaca atgttcttag caactcatcg ccaaattcaa
     6481 aattgaaatg gataccgctt accatctgtt ttcgcttccc gtattctttt gctaaataac
     6541 gtcgatacaa gacatcttcg aaatttttta attttgcaat aatgatcgct tcgtcttttt
     6601 ccggtaaggc aggtggcata cttaatggcc agatcatctc ttctttcggt atagaacgag
     6661 cagtcacatc ataaacagca gcgagatact gaaataattc gggaatagaa tcggtaacag
     6721 gagtgatcat ctctgtttgt gtttcgctaa aatctgtttg gatatagggg tgataggatc
     6781 gatcgccaaa gattgctgga tgatcggttt ttgctagatt tcctgcaaga tcaacccgct
     6841 gtccttcccg ttctatacca aatcgagctt ggtcgataaa agggcgcgca ttgacatgca
     6901 ataataattg cttaaaattc atcattaagt gacctccaat tcatatgaat ctaattataa
     6961 tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc gcttacaaat aagtataaaa
     7021 atgaaggcat caaaaacaca gctatgttaa aaacaatcat tcaaacaagg agctgcaact
     7081 tttgtcacag ctcctgcttt ttagacatgg gttattcttc ttttagtaat cctttgaaca
     7141 atccaacgac aaaatcattt ggatcaaacg gctccaagtc atcgattcct tcaccaagac
     7201 cgaccaattt cactggtaaa tgcaattcat tacggatagc aagtacgata ccgccctttg
     7261 ctgtaccatc taattttgtc aaaacaagac cggtcacatc tgttgtttct ttgaattgtt
     7321 ttgcttgaac catcgcgttt tgaccagttg ttgcatctag tacaagaagt gtttcttggg
     7381 gtgcttctgg taattcccgc tggattactc gttttatttt ttctaattcg ttcatcaagt
     7441 tcactttatt ttgcagtcgg cctgcagtat caactagtaa aacatctgcc tgctctatct
     7501 ttgctctttc caaagcatcg aatacgacag ctgctggatc gccgcctgca ttgccacgaa
     7561 caacttcaac accagcgcgt tcgccccaga caactaactg gtcgattgct ccagcgcgga
     7621 aggtatctgc tgctgccatc aatacttttt taccttcgtt tttgaactga tgggcaagtt
     7681 taccaatact tgttgttttc cctacaccat taacaccgac aaacaaaata actgttagtc
     7741 cgtcttcttg gatatttagt tcatttactt cattgattcc ttccgcttca tagagatcca
     7801 caagtttctc gatgatcgca ttttggactt ctgccgattt tttcgcattt ctgagtttca
     7861 cttcttgacg taatgcttcg gtgattcgta tcgctgtttc aaaacctaca tctgcaccga
     7921 taagtgtttc ttctaattct tcaaaaaagt cttcatcgac tgttcgaaaa ttggcaaaca
     7981 actcattcat ccgttcacca aatgttttac gcgtcttttc taaacccttg tcgtacttct
     8041 cctgtacttc aacttcttct gttttctctt cgccagtaaa agcacgcttg attttatcaa
     8101 aaaatcccat ttgactcccc cctactctaa tacaaatttt tggatcactt tggctacgcc
     8161 attttcttca ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag gcacagcatt
     8221 ctccatcgct acgcctagtc cagcgtattg gatcatcggt aaatcatttt cttcatcacc
     8281 gattgccatg atttcttctt gtttcaaacc aagatctttt gccaataaag aaataccata
     8341 agcttttgta acaccttttg gcataaattc caaaagcata cttctcgttt tgatgatctc
     8401 atacatttca tggaattctg ctggtatttc tttgatttta ggatctaatt cttcttggtc
     8461 ataagcgatt actgccttat taagcacaaa ttcatctgta atatcgctta tatccgctgg
     8521 ttggaattgc agcagcttat tcaacacatt gtacaaggat tgcttccttg gggcactagg
     8581 cagctgtaaa accacattgt cagacaaaac atctagtggc agatccaatt gctgagccag
     8641 tttgtataag cgttggatat ctgatacttc catcactttc ttttccatga tttcaccagt
     8701 atcatttttt tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt catctaccag
     8761 tcctaattct tgcagataat gcgccatcgc agctaagggg cgtcctgtac atagaacgat
     8821 cttgacccct tgctgttttg cttgtgctaa tgcttgttta ttctcatctg aaatcagctt
     8881 ttgttcattt aataatgtac cgtccaaatc gattgctact aatttgatca ttttcgttcc
     8941 tacactttcg tttcgttgat tttcgcctct tcattgatat cttccatccg tacagaaacg
     9001 atttttgata caccggattc ctgcatcgtc actccgtaca gtacatctgc tgcgaccatc
     9061 gttcctttgc ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc gctcaagtaa
     9121 cgaccaaaac gtttcacgtt ggcttcatct aacgctgctt ctacctcatc taagatacaa
     9181 aacggaacag gacagacacg gataatagaa aagagcaacg cgatggctgt aagtgctcgt
     9241 tctcctccag aaagcaaact taaactttgc agttttttgc caggaggttg tacttcaatc
     9301 tcaatacctg ttttcagcaa atcagttggt tctgtcagta ctaattcagc tttaccgccg
     9361 ccaaacatat ttggaaagac aattttgaat tcttgacgga tagcttcaaa tacttctttg
     9421 aatcttgtcc gcacttcatc atccatctca tccatcgttt caaacaattg gtttttcgct
     9481 gctaacagat catctcgctg tgttgctaag aatgtatgtc tttcactgac ttgttcgtat
     9541 tgttcgattg aatttaagtt tactggtccc agtttttcaa tctgttcttt taaggatgaa
     9601 acctttgttc tactgtcttc gatatcggtt gtttcttgat agtcttggga agctttctca
     9661 aatgtcagct gatattctga ctgcagatag gtcaaatgat tatctaaaat catttcagct
     9721 cggtcttttt ggacttctaa tttggattgt tccgacagtc gtgctttttg ctcacgatta
     9781 cgctctgcca agactgcttc aagcttatcg atctcctttt gccttctatc tcgctgctct
     9841 ttggcttttg ccaactcggc tttcaatgtt tctcgctgag cagataattc gttaatttgt
     9901 ttttccaaac tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa tgtcgccaac
     9961 tgcttttcta tcgcttcttg acgttcggtt gcctctgctt tttggactcg ggcaccgcgg
    10021 agctggatct gcagatgatt aaactgctct ttcaagacag caagatctgc ttgttcttgt
    10081 gctttttgac tttgtacttg tgctcttctg gcttccatct ggtcgctttc ttgacttaac
    10141 gatttgattt cttcgtcgat tttttgtcgt tgcgattcaa gatctgtttg tttgtctttc
    10201 aattcgttcg tttgcttttc gtaatcttcg ataaactgct gcagttcacg tgtctcaaaa
    10261 gttgagattt gcttttcttt ttcaaaacgt tcaagttcat ttatgatgtt ttgcagctga
    10321 tttgtcgctt cttgttcttc aaaacgtaat tgttcgcctc gtgtacgcaa aacttcttgt
    10381 tcttcggcta aacgagctgt ttcttgttgc agctcctgaa cttttttttc ttgtgcttgg
    10441 agttgtttat ctgcttcttc aaattcacta gtcaactgtt tcaattcttg attttgtaca
    10501 aacaaactgc cttggtttcc tcgtttattc gctccacctg tcatggatcc tccggcattc
    10561 atcacatcac cttctaagga aaccactcga tattgatagc ggatcgtttg cgcgattgca
    10621 ttggcactag ttaagtcttt ggctaaaagg atcgttccta gcaggttttg aaccacagtt
    10681 tggatttgat tgggataact gacttgttca ctggcaatcc ctataaaccc ttctacagct
    10741 gctgcttgtg tcaatatatg agccggtaat tgtcttggtt tgattgtcgt cagtggaagg
    10801 aatgttgctc ttcctcctct ttgctgtttc aaatacgtga tggcttgccg agcatctttt
    10861 tcattttcaa cgatgacatg ctgggctgcc ccacctaaag ctgtttcgat agctaacgtg
    10921 aaatctgcag gaacatcgat cagttcagcc actgctccga caatacccga aagctgctgt
    10981 ttatgctgca agaccaatcg aaccccttgg taaaaaccaa aatagttttc ttggatttct
    11041 tgaaggcttt tcttacgagc acgtagctgc tggacttgat tcattaattg atacatttta
    11101 ggctgttctt ttgctaatgc ttcttggaac tttttcccgt tttgttggat catttctaac
    11161 ttctcttttg tttcagttaa agatgatttt agctgcgcct gcttttgtgt caattcttct
    11221 ttttgtaaga tcaatcggtc tactgatgcc tctacttccg attgcttggc taacgtctgc
    11281 ttactttttg ctgtttcttg tacatattga cgttctagat acttcagttc gtttcctaca
    11341 gctgcttgct cttgcatcaa atcaacatat tgatctctta gttctgccag taattctttc
    11401 gttgattttt ggtatttttc taattcttgc tgtgttttta tgagacttgc ttcagctttt
    11461 tggatttctg tttctttttc tgctacttct ttcaacaagc tttcttgaag cttttcaaaa
    11521 tgtttaacct ttttttgtgc ttctgctaat gaagtttgat attcttgcga gcttttttgt
    11581 gtatgctttg tccgctcctg caaaacatct ttttgtcctt ctgtctgctt cagtttttcc
    11641 gaaagatcta ataatacttg ctgatttttt tcgatcagac gatcggcttg cgcattttct
    11701 ttacgctgct tcgccaaaat ggattcttgt tcttggatcg actcagatag tttgcctaat
    11761 tctagattga atttcgcaag ctgtgcttgt ttgttgtccc aatctttttt tgctgtcttg
    11821 atttcggcaa ccatcaaaga gacatcggtt tgagtcagtg tttcttttag ccgtaaaaac
    11881 tcctttgctg cctcactctg ggcggccaaa ggagttaact gttcttccaa ttcgtggatg
    11941 atgtcttgga cgcggctgag gttgtcttct gtttcaaaaa gcttctgttc tgctttcttt
    12001 ttccgctgct tgtatttcaa gacaccagct gcttcttcaa agatcccacg acggtcttcc
    12061 ggtttactac tgaaaatcgc ttctactttt ccttgagaaa tgattgagaa cgattctttt
    12121 cctaatccag agtctaagaa caattcttgg atatctttta atctgcagct ttgtttattg
    12181 atgaaaaact cgctttcccc agtccgtcga taacggcggg ttacactgat ctcattgtac
    12241 tccaatggca gataatgatc actattgtct aaaatcactg tgacttctgc aatattcagc
    12301 tgcttacggg tatcggaacc ggcaaagatg atatccggca tttttccgcc tcgcagactt
    12361 ttagcagatt gttctcctaa tacccagcgg atagcttctg taatattgct ttttccactg
    12421 ccatttggtc caaccactgc tgtgacgcta ttttcaaagt caatgactgt cttgtctgca
    12481 aatgatttaa aaccggctat ttcaattcgt ttcaaataca aagctttccc tcacttactt
    12541 ttacagtgct gccaatgcat tttccgccgc agcttgttct gctaattttt tcgattttcc
    12601 ttgtccagtg ccaatcaatt gatcatctac ataaacttcg atccaaaata ctcgttcatg
    12661 agctggacct tcttcattga tcaatcggta ttcgattgat acatcacctg atttttgcaa
    12721 gacttcttgt agttttgttt tatgatccat ctcatgtgaa aaagcacctg ctttgacttt
    12781 aggaaaaatc actttttcga tgaatgcatg ggtcgtgtcg aatccctggt caagatataa
    12841 cgcgcctaaa aaagcttcga ataggtcgca aagcaatgct ggtcgcgtac gtcctcctga
    12901 attctcttct cccttgccca acaaaacata ttgatcaaag tgacattctt tggcaaattt
    12961 cgacaagctg tcttcgcgaa cgatcgcagc tcgtgttttt gtcaattttc cttctggcaa
    13021 atgaggatat aaacggaata aatactgaga aaccatcagt tctaaaacgg catctcctaa
    13081 aaattcaagt cgctcattat ccgataattg cagattgcga tgctcattca cataggatga
    13141 atgggtaaaa gcttgttcaa gcaaatttag atcgtgaaaa acgatgttat agttttcttt
    13201 tagttctttt gtcagctggt tgtccatttt ccacattcct tttatggata ttttttcgat
    13261 acttccttat tatactggtt ttttgtcaaa tttgaaatgg ttcggtcaaa aggtaagtaa
    13321 agctttaata atcctagtga ttgacgagaa gttgtgaaaa attcgtttag tttcgagtat
    13381 gaagaaaaaa ttttgtgaaa atggctttcc atatttttgc ataaatttga cttaataccg
    13441 agaaactgga ctttgaacac cgtttattgg agaagtcatg aaatcagcgc cttgacctga
    13501 atattagaga aataaacaga aaaatatcaa aaaaccgaaa aaggacatcg ccctttttcg
    13561 gtcattttta gtgattatga acgtgaatct gctgtaacag aaacatttgc ccatgggttt
    13621 ggtacatcat aagaccaatc ccagtctttg acacggtcgt tgactggcaa tacttcatta
    13681 cggaacaacg ttggaatcgc aaatgcttct tcaaatgcat attgttgcca tttttgatat
    13741 gcttcttttt ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt gatcaatttt
    13801 gtattttctt ctgattcata acgagtgtag ttgaacgcag catttggtcc ccatagtcca
    13861 cttggatttg gatcagttcc tgtattccaa gcagcttggt aaacatcaat ttcaggatca
    13921 tcgttttcta atttatcata gaaagaattg aattcgatca gacgacctgt tgtgtattgg
    13981 acatccaaac cgatttcttt ccactgttgg atatagtaat ctgccaatgg ttgcgctgtt
    14041 tccccaccgg acatcgaagc aaagttgatt gttagtttat ttccatcttt gtcttctctt
    14101 aaaccatcgt catccacatc tttgtagcca gcatcatcta atagtttttt cgctttgtct
    14161 agatcttgtg tgaatccttt catctcatca tcatggtatg actggaatac tggaggaatc
    14221 aatgaagtcg cattgcttct caaacctgca tagaaacgct cacctactgc attattatct
    14281 aatgcatagc ccattgcttg acgtaatgat ttatcagcca tttttgaatc tggattgtat
    14341 tccacactct ttgtatctgc attccattta cctaacttga atcctagata agtataagaa
    14401 gtttgttcac ggcctaagtt tgtatagcca gcaatttctt tataggtatc gtaagtatct
    14461 gttggcattt ggaaaatcat atcatataat ttagagttca atgcttcagt tgcacttgcc
    14521 atcggtacag atttcatcgt gatctttttc aatttaggcg taccattcca gtagtgctcg
    14581 tttggcaagt attctactga ttctcctgca acgatattac tcatgtaata agggccaaaa
    14641 gtcactggat ttttacgtac ttgatcgctt gattccatat ctttgattgc tacaccttct
    14701 aacgtatgtt ttggtgctgc atatgaccaa ataccaccac ccgattgaag catgcttggg
    14761 tttacttctt tatattcgat ttcaactgtt ttgtcgtcta tttttttgat tccggagatt
    14821 gtgtcggcac ttccatcatg atattcttcc atacctacga tgttacggaa agtgtcgtca
    14881 taacggatac cagtgtaatc tttattccca atgatttcgt aagggaaaat cacatcgtcc
    14941 gccgttacat cttcaccatc tgaccatttg acgttgtctt ttactttgat tgtcgctttt
    15001 ttcgtatctt catctagatc aagcgttgca gctccgtcgt ctgtgattct gaaatcgtca
    15061 tcgtttgcaa atagcggttc atgagaagga gccataaaag ctgcatcata tgaatcttgg
    15121 taaaactccc actggaacaa tcctttaaac tgactgtccg ttgcgaccgc agcttctaat
    15181 tcgccgtctt cgattgcatc atcattattt tttgttgcaa ttggaaaagt taattcctct
    15241 ttgttattgc cgttgcctcc agagttgttt ccagaggtat ctgtacctcc gcccccgcat
    15301 gctgcaagaa cgacagcaga catcagcgct gcaaatccaa aaatcgtttt cttcattcct
    15361 atttccccct tcgcttctat actagcctaa ccgttggcgc gcatctgcag aacgcttgaa
    15421 tgcttgtcca acataattga tactcaacat caaaactaaa ataaggattg atgcgggtaa
    15481 ccagatccat tgccgattca ctaagacttc tccgttactt gcataaccga tcaatgtacc
    15541 taaactaggt acatttgccg gcaagccgaa acctaagaaa gtcaaagttg tctcgatacc
    15601 gatgtttgcc gcaaagttca tcgtcaaatt agtaatgatt aatgaactta gattcggcat
    15661 gatttcgcgg aacatgattt taaaatcatt ggttcccatt gtttttgagg cactgacata
    15721 atctcttctt gcttcagata atgtcttgct tcggaataat cgggctttag ctacccaata
    15781 aaatgcactc atgatgaata taaaggacca gacattgtat ctggaaataa tcgatacgaa
    15841 gacgataatg atcagcatga tcggtagaat catgataaaa tcaacgatcc gcatcaagat
    15901 gttgtcaatg atccctccgt agtagccaga gatgatccct aatccaaccc cgatgatcga
    15961 agtaaggatc gtgatcgcaa atccgatcgt aacagagttt ctagcaccta tgatcaattg
    16021 tcctaataca tcacgtccgc cttcatctgc tcccagaatg aacttttgtc cttgtgtcga
    16081 aactgttcct ggtgcagcgt atttgtcgaa gatactaaca tacatcactt tgtcttgatc
    16141 ggtcagcatc gcaccgataa agatcactaa taataaaacc actagcaaac ctaatgaaaa
    16201 aatagcaatt ttatcttttt taaattctcg agcaatcatt cgtatcccca taggaggaat
    16261 actttcttga actgctgtat tgtttttttc actcatcgat ccattctcct ttctgattgg
    16321 ttttattgaa tccgaatacg cgggtcaacg atactcatga tgatatctga aagtaatgtc
    16381 cccactagcg tcgcgattcc taaaatcaat actaaggatg tgatcacggt atagtcccgt
    16441 tggccgatgg aatcaataaa tagttttccg actcccggat aaccaaaaat cttttcgata
    16501 actacagaac cagcaatcaa cgaagtgatt tcatacccca tttgagaagc catcggcaac
    16561 gctgcatttc ggaaaatatg tcgactgaat actttattcg tcggtacccc tttggaacgg
    16621 gctgttctga caaaatcgag cgatttcgaa tcgattactt cacttcgcag atattggatt
    16681 gtgacggcag tcgccaaaaa tgcttgggta attgatggta acagaatata tttcaaacgg
    16741 tctatccatt gagtgataaa tccaccttgc aaacctagtg tttgtgatcc acttgttggg
    16801 aaccagccta aacgataacc aaaaacaaat agcatgatca agccgaagat aaataaagga
    16861 acagcaaaac taaagaagtt gtagattacg acaatcttat ccaagattga attttgatac
    16921 cggccagcaa gcatccccag aggcacagca atcaaataag taaggacaac tgtcaagatc
    16981 gatagatata aagtgttgat gatccgttcg ccgatcaagt cagcaacaga gcgtttgtat
    17041 aagaaacttt gaccaaaatc gccttgcaaa gcatttccga tccaacggaa gtactggaca
    17101 taccatgggt cattcaagcc agctgcttgt cgcatctgtt cgatcactgc cggatcctga
    17161 tttggattga tcaaaccggt aaatggatcc cccggcatcg cttgggctaa taagaagatc
    17221 agaacactta aaataataac ttgcgggatc atcaataaaa cacgtcgcag aatcgttttc
    17281 cacattagct tgcacctcca tctttcaacg ccactttgtg tgtagttgtg atcgtacgca
    17341 aatcgtagac tcgaccagtc gcatcatagt attctttttg gttttggata tactcttgtt
    17401 ctactcttcg tcgattttct ttgtggcttt ccctattttc tacatcgatt tgcggaatag
    17461 ctgaaagcag acgtttcgta tagatatgtc gcgggtcatt ataaatgtct tctcttgtac
    17521 cgatttctac aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg tgttttacta
    17581 cacctaaatc atgtgaaatg aataaatagc tgaggccgta ttcttgctgg attcgtttca
    17641 tgaaattcaa tacttgcgct tgaacagata gatccagagc agatacaggt tcatcggcta
    17701 cgatcagttt tggattggta gcaacagcac gggctactcc tagacgttga cgttgtccgc
    17761 ctgaaaattc atgaggatat ttatatagcg catcttccgg catcccaacg atatctagca
    17821 gtccttttac ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt cggatgggtt
    17881 cagcaatgat atccaacact cgctttttag gattcaaact ggacattgaa tcttggaaga
    17941 tcatctgcac atccttgtta taacccattt ttttgcggtt gcttcgcttc gttacatctt
    18001 ttccttcata catgatttgt ccggctgtca ccttttctaa tcccacaacg gcttttcccg
    18061 tagtagattt tccagagcca gactctccca ctaaaccata tgtctttcct ttttcgataa
    18121 taaaatccac accatctaca gcatatacat gatccgttac tcgattgaag aatccactgc
    18181 gaatagggta atgaactttc aggtctttga ttgtgattaa ttctgtcatc tacacctctc
    18241 ctttttcgtc tctaaagtgg aaatgcttat agcaggagca acgtaccaag tggttcggtg
    18301 cgacttcatg aaggataggc tcttcttcat gatcgcttgc tggtatccat ggaatacgtg
    18361 gtgcaaaccg acaaccttta cgaggcatgt ttttcagaga aggaacgaca ccttcgatca
    18421 catggagttc tgcatcatga tttccttctt gtggtataga gttcagtaaa gagcgtgtat
    18481 aaggatgctt aggatgatca aacagttctt ttacacctgc tacttcaacg aactgtccgc
    18541 catacatcac tgccactcga tcagccattt ctgctacgac tcctaagtca tgagtgatca
    18601 agatgatccc tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga atctgtgctt
    18661 ggatggttac atctagggct gtcgtcggtt catctgcaat gacgattgga ggtttgcagg
    18721 cgattgcgat tgcgatgatg acacgttgtc tcataccgcc tgatagttca tggggatatt
    18781 ggcgtccaac tctcgcagga ttaggaatcc ctacttgttc tagcaattct agtgcacgag
    18841 cttgacgctg ttctgcattc atctttgtgt gataggaaag tccttctttg atctggtctt
    18901 cgattctcat gagaggattt aatgcagaca acggatcttg gaaaatcatt ccgatatcat
    18961 ttccgcggat tttattatag agcgtttcgt tcaggtcgat taaattcaga tcgttatata
    19021 aaatctcacc tgtaattttt gtgttattag gatcaagtaa tcccataatc gtagtagcca
    19081 aggtactttt cccgcaccca gattccccaa cgattgctaa aatctcgttt ttgtctaaag
    19141 taaatgaaac gtcatctact gcatcgtaat agtcatcttt gagtcggaat cctgtatgta
    19201 ggttctgaac ttctaataat tggttatcgg acaatcgaat acccctctct gcttcctaaa
    19261 tggaagtttt tcttaataaa aaaggacatt acacagaata aagcgtttta taacaaaggt
    19321 tattcttatc tttgtgtaat gaattattaa taattatata gaaaatcgct ttactttgca
    19381 acataatttc tgtttatcgc cagaattatc agaaaaatga cttttacgtg tcaaaaagat
    19441 taaaaaaaga aggataaaaa gctgtatgca gctttttatc cttctttgct tttatttgaa
    19501 attatttaag atggctgctg atataatcta ctgcgccacc gactgtttca atttgttcag
    19561 cgtcttcatc tgaaatttcc gtaccgaatt catcttcaag ctctaaaacg aattccataa
    19621 tactaattga atcagctttt aaatcatctt tgatgttcaa ttcatccgtc actttatcgg
    19681 tatctatctc aaaatgattt gaaataattt tcgctacttt gttaaatact tcttcgcgag
    19741 tcaacttttt cacctccaag aatcatacac ttgtcaatta cctacaccat ggctaatttt
    19801 actggcattt ttcaggaatg tccacctttt tttcaatgga acggattgct tcagatatcc
    19861 aaatactatt cttcgctttt ttcaaattgt tcgactaatt gtccaactac atctgtctca
    19921 agcattgtgt ggatttggcg gatcgtatag cgaacagcat cggggcctgt tgctccatga
    19981 gttttaatga ctggtgcttt caagccaaac aaaacggcac cgccatgttt ggaataatcc
    20041 atttctgctt tcaaagaacg taagccgtct tttaaaagca tagcgcccat ttttcctttg
    20101 atcccttcat ttagaatagc agattttaat agattcatca tattcattgc tgttccttca
    20161 atagatttca aaacagcgtt gcctgtaaat ccatctgtga ccactacatc agccacacca
    20221 ttgagtagct cacgagcttc tacgtttcca ataaaattca agcttgtttc attctgaagg
    20281 agttcatagg cttttttcgt cacttcacta ccttttgttg cttctgttcc gttgtttaag
    20341 agtgcgacac gaggacgtcc gatacctcgg actttacgtg cataaaaaga acctaaaata
    20401 ccgtattgca acaaatgttc tggtttgttt tctgcgttag cgccaagatc cagcatatcg
    20461 aaaccggcac cttctttccc tactactggc aaggtagaca tcaagccagg gcgttcgata
    20521 tttttgatac gtcctacgat gaacaaccct gctgctagta gcgcgcctgt attccctgct
    20581 gaaaatacag catctgcttc accgtttttc actgcttgtg cagcaaggac cattgatgca
    20641 gtcttttttc gtcgaattgc ttttactggc tcatcatcac tattgatttt ttcgtctgta
    20701 tggataatcg tgatgttttt ttcatttgtt aagtatttct tgatttctgc ttcttttccg
    20761 taaagtaaga attctacttc tggaaaatct tgctgcgcta aagccacgcc ttctacaatt
    20821 gcttgaggtg cgtgatctcc gcccattgca tctacggcaa ttttcattgt ttccactcct
    20881 ttttccgtca tcagtataaa gtatagcaca gtctgctaat cgaaataacg attatcctga
    20941 ttttctattt ggaggctttg cgctaaaggc tgatactcag gaagcatctg ccaatccttt
    21001 atctgccaaa gggctgcagc ctcttctctg gctacctcca aaacatttcc atccgtcacc
    21061 aagtcagcaa tcgcaaattg aggcagacca gattgacgga agccaaatac ttcaccagga
    21121 ccacgtaatt ccagatcttt ttcgcttagt acaaaaccat tggtggtctc tgtcatgatt
    21181 ttcatccgtt ccacacctag ttcattttta ggattagcaa ctaagataca gtaggaagca
    21241 tcacttccgc gtccaacacg tccacgaagt tgatgcagct gtgccaatcc aaatcgatcc
    21301 gcatccatga tcagcatgac ggtcgcgttc ggcacgttta cccctacttc aattacagta
    21361 gtcgaaacta atatctgcat ctggttgtct ttgaaggctt ccatgattac ttctttttcc
    21421 tgattcttca ttttaccatg taacaagcca acttcatatt ttggtgcaaa tagttcacga
    21481 agtttttcat agatttccac tgcatttttt acatctaatg cttctgattc ttcaatcagc
    21541 ggacagatga catacatttg atgtcctctt gccagttctt ttttcgtcca atccagtaca
    21601 ctgtccaatt gaggtgttct tacccagcgt gtttcgatcg ggatacgtcc agctggaagt
    21661 tcatcaatga tagatacatc catctcacca tatgctgtaa tagctaatgt tctaggaatc
    21721 ggagtcgctg tcataaataa gacatctggt cgccagcctt tttcacgaag tactttccgc
    21781 tggttcacgc caaaacggtg ctgctcatcc gtaataacta gacctaaatt ctgaaattcg
    21841 actccatctt ggatcaatgc atgtgtgccg atcacgatgt taatttcccc ttgctctagt
    21901 ttttcaagga gctcccgacg ttcttttgct ttcgttgacc ccgtaagcaa tgcgatggtt
    21961 acttcattgg gatcaaacag ctgttgcaag ctttccatat gttgttgtgc caagatttct
    22021 gtaggcacca tcaacgcacc ttggaaacct gctgtaacgg tagcgtataa cgcaatcgcg
    22081 gcaaccactg tttttccgct acctacatct ccctgcaaaa gtcgctgcat atgctgcgga
    22141 ctgcgaagat cccgacagat ttcattcgtt acacgttttt gtgcatttgt cagttcaaaa
    22201 ggtagctttt gagtaaattc tttcaatttt tggacatcat aaaggatctc caaaccattc
    22261 gtttctgctt tttctgcttt tttcaatcct tgaagacgca tttgaaataa aaagaactct
    22321 tcaaaaacga cacgtcgttt ggcttgatgg ttttcttcgt ggtcttttgg aaaatgcata
    22381 cttcttacag cagaagctcg gtcaagcagt cgatatttct ccacaaggtt gtttggcaga
    22441 ttttcttcaa tcaatgtgcc gtattcttga aacccttttt tgattagatc aactaatgtc
    22501 gtttgacgaa ttgatttgtt gacgtgatat atcggcgaaa agtcatccat tgtttgagaa
    22561 cctaaaatct tcatcccgtt caatgcttta cgctttgcat cccatttacc gtatacggcg
    22621 atttcctccg ataaaatgac tttttctttt agataaggtt gattaaaaaa agaaacgttg
    22681 aacacatcat gttcttgcat catacgaaat tgcaatcggc tttttttata accaaatcga
    22741 ttcatcacag gaggcgagac tacgattcct ttcaaagtaa ccttttcttg atcttggatc
    22801 tcgtttagat ttctttcctg aatatcttcg tagcggaaag gataataagt cagtaagtct
    22861 tcaatcgttt ggatgcccaa gctggcaaga ctagctgctc ttttttctcc gactcctgcc
    22921 agcatagtga ttggatcatc taaagaacgc ataaattttt cctccctcct ttaacaaaaa
    22981 aaggacttgg tcagcgatca tacctgatca ctaaccgaag gccttttatg aatgaatact
    23041 gtgttagaaa gatattctga cacagaaatt attcagcaga aaatagataa ggatagacag
    23101 gctgatctcc ttggtggatc tcgatttcca aatcaggatg tttttctgtt agtttctcag
    23161 caaattcatt tgcttgttca actgttcctt catcacctgc caagatcgtt acgatctctg
    23221 tgtcctcatt gatcatttgt tctagtgtac ttaacgctgt tgctgtgata tctgggttag
    23281 agatcaggat ttttccatcg atcatgccca aaaagtctcc ttctttgatc tctacaccgt
    23341 caattgaggt atctctgatc gcgtgagtga cttgaccgct cacaacgcta gacaacattt
    23401 ctgtcatcgt ttctttgttt tcttctaaag aagcttgatc attgaatgaa agcatagctg
    23461 tcaatccttg ggaaatcgtt cttgaaggaa caactgctac tgggatatct gctacttctg
    23521 ctgcttgatc agctgccatg aagatgttct tattgtttgg caagacaatc actttatctg
    23581 cgtttacttc ttgaatcgct ttaacaatgt cttccgtact tggattcatt gtttgcccac
    23641 cactgataac ataattagcg cctaaacttt caaacagctc ttggacgcct ttaccagcag
    23701 cgatcgctac gataccatac gggatacgtg gtgtttcttt tttcgcaaac tcgttcactt
    23761 cttcatcgtg ttccaaaatc gtctcatgct gcaagcgcat gttgtcgact ttgattttga
    23821 ctaatgaacc gaatttttgt ccataattca agacttctcc tgggttttct gtatgcacgt
    23881 ggactttgat gatctcgtca tcattgacca caagtaaaga atctcctata ccatctaaat
    23941 agttacggaa ttcttcgtaa tcgaatgtac tatcgactgt aggtccttct ccgattttta
    24001 ccatgatttc tgtacagtaa ccaaaatgga tatcttctgt cgccatttga ccttggacac
    24061 tgcgatgatg ttctgcattg accatatcat ccatttctgc tggagaaggt tcatatgttt
    24121 catccgcttg gaattctcca ttcaatgcac ttaaaaagcc ctcataaaca aacagcagtc
    24181 cttgccctcc actgtctacg acaccgactt cttttaggac tggaagcaag tctggagttt
    24241 tagctaaagc acgctttgcc ccttttacaa cagctgtcat tacttcaacg acatcatcag
    24301 tactttttgc tttcttttct ccagctttcg ctgcttctct agctactgtc aaaatcgttc
    24361 cttctactgg tttcattact gctttatacg cagtttcaac tccatgagtg aaggctgcag
    24421 caagatcgga agcatttaat gagacaacat ctggaatttg cttagaaaat ccacgaaaca
    24481 attgagacaa aataacgccg gaatttccgc gcgcacccat cagcaatcct tttgaaagca
    24541 ttgctgctaa ttctcctact ttttctgagg ttgaatctac tactgcctta gctccactag
    24601 tcatagatag attcatattt gttcccgtat cgccatctgg cacagggaat acatttaatg
    24661 aattgacgta ttctgcattt acttgcagac gattcgcacc tgcctggacc atttcctgga
    24721 actggcttgc acttatttct gttacactca cctagataat cctccttcag atcgactgct
    24781 gagttaccag ccagtctcct gcctagtctg gcagtacacg aacaccttgc acaaagacat
    24841 taactgaatt ggcagttacg cctaataatg cctctagatt gtatttcact ttttcttgga
    24901 cgttgcggga aacttcagag attttcgtac catagctgat aatcgtataa acatctacag
    24961 caataccatt atcttcttgg cggacaacta ctcctcttga gtaattttct ttacgcaaaa
    25021 tctcagtaat attatcttta atttgatttt tgctggccat tcctacaaca ccgaacacat
    25081 ctgtcgcggc gccaccaaca actgttgcaa ttacatcatt ggtaatttca atggttccag
    25141 ctgacgtttg aatttttaca gccatgaatg agcctcctta acgggtaaaa aactcccatt
    25201 tttcttctat catattttat catagcgcaa cttgaaataa aaggcagtta cccaaagtac
    25261 tcaaaaacct tgcaaaattt caggtataac cttgtttaat ttacaattat tcttctcaat
    25321 tgtcaattgt atctatgaaa atgtacttgc aaatttccat tctatatgat atgttagtct
    25381 ggtatgagct gaattattct attgctccaa atcaaggcaa aggaggaaat taccatggca
    25441 aaagtttgtt actttacagg tcgcaagaca aaaagtggaa acaaccgttc acacgctatg
    25501 aactcaacaa aacgtactgt taaacctaac ttacaaaaag ttcgcgttat ggtcgacgga
    25561 aaacctaaaa aagtttgggt gtcaactcgt gctttgaaat caggaaaagt tgaacgcgtt
    25621 taattgaatg atacttggac cgttcagata gtgaacggtt ttttatttaa taaaaatttt
    25681 taagggtggg acttttgatt cctacccttt ttttgcgttt atttttgtta tacgaacaaa
    25741 ttatgaagtt atttattcta aattttattt ttttgtgaaa actagactct ttttaagcag
    25801 agaaaaacaa ggatatgata aactgaatac aacttcatag aaatttggtg gaaaataaat
    25861 atgaaaaaaa atttaaagtc attctttaga gaaaacggac gctacgcagc agctagtttc
    25921 ctgattccgt ttttgatttt agcacttgtt tatctaagta tcgggatcta tcctggaagc
    25981 agcagaagta ttttggcaag tgatgccttt tcacagtttt ctaattttca tgccagcttc
    26041 cgaaatgttc ttcttggaaa gcaaagtatt ttttatacat ggaatgcttc tctaggattg
    26101 aattatttat ctttaatttc ttactatcta ggcgggatct tcacgccact ggtgttcttt
    26161 tttccaaacc agatgatgcc agatgcactg tatgttctga ctttgcttaa ggtcggctgt
    26221 gccggattgg ctttttggtt tcttgcacgt acgttcaaga ttcctcgctg ggggcatgtt
    26281 gcacttagcg tcagttacgc attgatctca tttatcactg cacattcgga gatcatcatg
    26341 tggctggatg catttgttta tcttccatta gtgattttgg ggattcatcg tgttatggat
    26401 cttaaacggc cgaaactttt atttatcagt tatttactct tgtttttatc cagtttttat
    26461 atgggtttta tgattggcgt attttctttc ttatatttta tcgcccgtct attgacaaac
    26521 tggtcagaat ataaaaaaac gattgttcct tatggtatca cttctttact ggcaggcggc
    26581 gcttcaatga tcattgtatt gccagcagtg ctggatcttc ggtcaaatgg cgaggcactg
    26641 agtcagatca ctacgttgaa aacagaagcg acttcttatc tggatctaat catgaaaaat
    26701 atgattggtg tttatgacac gacaaaatac ggatcgattc cgttcgttta cgcaggtctg
    26761 ctaccattag tcctctgtct gttttatttt gccagtcgga aagtaccgtt gaaaaataaa
    26821 tttttgtacg gcagtctttt cgccatactt atcgcaagtt tctatcttgt tccattgaat
    26881 ttgttctggc atggtatgca tgcaccgaat atgtttttat tccgttatgc ctatctgttc
    26941 tctttcttag tgctcctgct tgcaggaaaa ggatgggaaa agatagaaca agacgatcgt
    27001 gggctgttgt taggaatcat tttggtcgta gctgctctct ttgcggtggc atggggcatc
    27061 aaacctgaag gaagctacac ctatgtgacg atgacttcct ttgtattgac agtggttttc
    27121 ttagggattt atgcttttgc tgtgggattt tatcagcata aaaaactttc attcaatcat
    27181 ctgagtatat tgttattatt gatcatgtct gctgaagcac tggtcaatac gaacagtatg
    27241 atccatggca ttcttgatga ttggaattat gcttcccgca gtttatatac tgatccttat
    27301 cctgacttga aagctcttgt aaatgaaaca aaaaaagatt ccgactcgtt ttaccgcttg
    27361 gaaaatctgg atccagtatc tgcaaacgat tcgatcaact atggttacag tggtatcagt
    27421 ttgttttctt ctataagaaa tcgtaattct tcctcttacc tggataaatt aggtttccgt
    27481 tcaagaggga caaatttgaa tatccgttat aacaataata ctttgctaat ggatgggttt
    27541 acaggaatca aatataacat ttctaaaaac gaaagcacgt tcagtaaata tggcttccgc
    27601 caagctggtc aaagtggcga ctatacactg tatcagaatg tcaatgcatt gccactagca
    27661 tttactgcac cgttgtccgt taatgatgtt gagcagccag tgacagataa tttgaccagt
    27721 cagacaaact tgatcaatac attgtctggt ttaaatgaac agtattttac attctataca
    27781 cctactttga agaatcagtt gaatacgacg atcacaaaga caacgactgg cgtcacttac
    27841 tcagaaacag cagataacca gccaaaagag ctcacttggg aagtccatgt gcctgcaaat
    27901 acacaagctt acttaagttt gttcccaacc aattttgcac aattagaaag ttctagtgcg
    27961 acgatcactg tcaatggtac atcgcgaaaa acgcaaatca acatttctgg tcaatactat
    28021 gatcttggct attatcctca agagacaaca gtgacattta ctgcaagttt ttatggaacg
    28081 aaagaagtaa gtttcatgga gcctaaagtc gttggtctag atgttgtagc ctatcaagcc
    28141 gcaatgaatc agatcaaaga aaaaggtgtc gaaatggaag ccactggcag aaaagcttct
    28201 gggactgtaa ataccgctga agattccatg ttggtgacaa ctattcctta tgacaaagga
    28261 tggaaagcta aaattgatgg gaaaccagtc aaggtcagca atttcaaaga cgcattcttg
    28321 atgataaaag ttccagcagg taaacatact gtcactttca gttacttgcc tgaaggattt
    28381 acgataggtg caatcctttt tgtcctatgt attgcattgt ttattcttta tgtctggtac
    28441 cttgaccgca atcgcccctt ttatggcacg attgatccag caatagaaga aaaaccgcga
    28501 aatacacaca gaagaagacg aagaaaataa agaaacgatg gaatctgtgt cactcttcta
    28561 tttaaatggc aaaaatttca atctttaaat gacaaaaacc acctgatttt tggtggtttt
    28621 ttatatacct atgactaaac tatctcttgc tgcttcgaca acttctacag tgatcttatc
    28681 catttgattg attcttaaaa tccgatcaat ctgcgcaaat aaacgatgaa taagcctaaa
    28741 atttcctttt gtgatcttaa tgatagttgt gatagcctca taatcagaaa aatcttctag
    28801 cttgatatca attcctaaat ctgcccattt gtattccaaa atatgatgtg tttcgtcctt
    28861 actaaggtta tcaaattcat gtgcaaatcc tatgcgagag taaagctgcg gataacgaga
    28921 caatcttttt tcaataccag gcataccgat aagaaccatt gccagatcat ttctatcgta
    28981 aatatctctt agctgttcta aatgctgaag ctttaaacga tcaatttcat cgacaataat
    29041 taagtcaatt gcttcatagg ttttaggcac aggaggatct tcttgattgt attttagtac
    29101 catatgcatt gctttagtca gacctattct actagttatt gtgttaatat ccgaactgac
    29161 acgagacatc ttttcagctg gtacagtata aaaaacttta tttacactta atatttcatc
    29221 tgttgcattt atcaagaaca agaaaaagtc tgaatattca aaaaaataac ggtgtacatt
    29281 tttaaaagga gatgtataat cgagattgag aaagcgtttt cctcaaaatt taatgaatta
    29341 ggaagtgatt actatgaaaa aattatttag cgcacttttt attctgttat ttggttttat
    29401 tggtattggg acttctggaa atatagttaa agctagcacg atggttgaac aaacaagtga
    29461 aatggcaaaa acttttagtc aattaacacc agaagaacaa agttacttca aatccaaagg
    29521 atttagtgat aaagatactt tttacactca aaagatagag cctcaagatc cgaaaacacg
    29581 tttttctgca aatgtattgc ggttaaccgc atctactaaa aaaattacta atacgcaggg
    29641 gcggacagaa tatattataa caagtcccaa ttcggcgatg tatcgtattt ctacacgctt
    29701 gagtttaggt agtgcaaaat catatactca aacagtaaat ctagctaatt cttatggtta
    29761 tggtggagga atgtatttta catttactgg gaaaagacaa tattacagtg taaaattagc
    29821 tatgcaagtt tataatacat ttggtgtagg gtcagtctct tgcactgcgg gaggattgac
    29881 aataggaaaa tgataatttc ttactatgtc ttaaatagaa gtgatgatga aggactcttc
    29941 gtttttcaat cattacatga ggatacgatg ctaaaagagt tttataatcg gattaagaat
    30001 aaacagatag aaacaaattt atatttttta agtgactcta tcttgccttt tattagcaac
    30061 cctaaaattc ttaacttgtt gcagacaaaa gatttgaaaa gtttaccgat tattgtcatt
    30121 gatgaggaaa ttgtaaaata tggcgcttat ctaagcgtag aagaattaga aaaattaact
    30181 aacattggaa tgagtattca ggaggataat ggggtacatg gaacatagaa tgaatatgca
    30241 cagttgagaa cggaatttga catttattta gaccaacagg gtataccctg ttggtctttt
    30301 tgtgcgttca tgatatactt ggtgtcattt ttatatttaa atggcacaaa tatttctaat
    30361 tatt
//
LOCUS       DAJSVF010000028        29107 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSVF010000028 DAJSVF010000000
VERSION     DAJSVF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.029"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..1029)
                     /locus_tag="ON550_001264"
     CDS             complement(<1..1029)
                     /locus_tag="ON550_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3954359.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="ON550_001265"
     CDS             complement(1111..1800)
                     /locus_tag="ON550_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3954360.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="ON550_001266"
     CDS             complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="ON550_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR3954361.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2327..2830)
                     /locus_tag="ON550_001267"
     CDS             complement(2327..2830)
                     /locus_tag="ON550_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR3954362.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3034..3161)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="ON550_001268"
     CDS             complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="ON550_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR3954363.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="ON550_001269"
     CDS             complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="ON550_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR3954364.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4797..5465
                     /gene="sdaAB"
                     /locus_tag="ON550_001270"
     CDS             4797..5465
                     /gene="sdaAB"
                     /locus_tag="ON550_001270"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR3954365.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5478..6350
                     /gene="sdaAA"
                     /locus_tag="ON550_001271"
     CDS             5478..6350
                     /gene="sdaAA"
                     /locus_tag="ON550_001271"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR3954366.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6441..7460)
                     /locus_tag="ON550_001272"
     CDS             complement(6441..7460)
                     /locus_tag="ON550_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3954367.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7633..8538)
                     /locus_tag="ON550_001273"
     CDS             complement(7633..8538)
                     /locus_tag="ON550_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3954368.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="ON550_001274"
     CDS             complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="ON550_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR3954369.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9243..9413)
                     /gene="secE"
                     /locus_tag="ON550_001275"
     CDS             complement(9243..9413)
                     /gene="secE"
                     /locus_tag="ON550_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR3954370.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="ON550_001276"
     CDS             complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="ON550_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3954371.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9751..10395
                     /locus_tag="ON550_001277"
     CDS             9751..10395
                     /locus_tag="ON550_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3954372.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10451..10954)
                     /locus_tag="ON550_001278"
     CDS             complement(10451..10954)
                     /locus_tag="ON550_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3954373.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10967..11013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11551..12516
                     /locus_tag="ON550_001279"
     CDS             11551..12516
                     /locus_tag="ON550_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3954374.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12615..13787)
                     /locus_tag="ON550_001280"
     CDS             complement(12615..13787)
                     /locus_tag="ON550_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954375.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13774..14919)
                     /locus_tag="ON550_001281"
     CDS             complement(13774..14919)
                     /locus_tag="ON550_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954376.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14933..15865)
                     /locus_tag="ON550_001282"
     CDS             complement(14933..15865)
                     /locus_tag="ON550_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954377.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15982..17061
                     /locus_tag="ON550_001283"
     CDS             15982..17061
                     /locus_tag="ON550_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3954378.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17058..17687
                     /locus_tag="ON550_001284"
     CDS             17058..17687
                     /locus_tag="ON550_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954379.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17797..18024)
                     /locus_tag="ON550_001285"
     CDS             complement(17797..18024)
                     /locus_tag="ON550_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3954380.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18127..20715)
                     /locus_tag="ON550_001286"
     CDS             complement(18127..20715)
                     /locus_tag="ON550_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR3954381.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20735..21394)
                     /locus_tag="ON550_001287"
     CDS             complement(20735..21394)
                     /locus_tag="ON550_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3954382.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="ON550_001288"
     CDS             complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="ON550_001288"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR3954383.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22167..23033)
                     /locus_tag="ON550_001289"
     CDS             complement(22167..23033)
                     /locus_tag="ON550_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3954384.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23142..23783
                     /locus_tag="ON550_001290"
     CDS             23142..23783
                     /locus_tag="ON550_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR3954385.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="ON550_001291"
     CDS             complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="ON550_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR3954386.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25224..26123)
                     /locus_tag="ON550_001292"
     CDS             complement(25224..26123)
                     /locus_tag="ON550_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3954387.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26125..26922)
                     /locus_tag="ON550_001293"
     CDS             complement(26125..26922)
                     /locus_tag="ON550_001293"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR3954388.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26900..27604)
                     /locus_tag="ON550_001294"
     CDS             complement(26900..27604)
                     /locus_tag="ON550_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR3954389.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27735..28310
                     /locus_tag="ON550_001295"
     CDS             27735..28310
                     /locus_tag="ON550_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR3954390.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28428..29105
                     /locus_tag="ON550_001296"
     CDS             28428..29105
                     /locus_tag="ON550_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3954391.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6169 c         5232 g         9142 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
     1861 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
     1921 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
     1981 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
     2041 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
     2101 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
     2161 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
     2221 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
     2281 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
     2341 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
     2401 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
     2461 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
     2521 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
     2581 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
     2641 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
     2701 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
     2761 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
     2821 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
     2881 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
     2941 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
     3001 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
     3061 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
     3121 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
     3181 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
     3241 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
     3301 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
     3361 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
     3421 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
     3481 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
     3541 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
     3601 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
     3661 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
     3721 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
     3781 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
     3841 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
     3901 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
     3961 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
     4021 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
     4081 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
     4141 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
     4201 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
     4261 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
     4321 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
     4381 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
     4441 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
     4501 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
     4561 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt ctgctatttt
     4621 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     4681 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     4741 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     4801 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     4861 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     4921 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     4981 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     5041 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     5101 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     5161 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     5221 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     5281 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     5341 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     5401 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     5461 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     5521 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     5581 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     5641 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     5701 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     5761 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     5821 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     5881 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     5941 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     6001 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     6061 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     6121 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     6181 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     6241 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     6301 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     6361 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     6421 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     6481 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     6541 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     6601 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     6661 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     6721 gcaggacctc catctaattg acgtccacca tgattggata cccagatacc atctgcccct
     6781 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     6841 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     6901 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     6961 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     7021 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     7081 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     7141 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     7201 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     7261 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     7321 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     7381 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     7441 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     7501 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     7561 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     7621 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     7681 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     7741 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     7801 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     7861 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     7921 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     7981 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     8041 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     8101 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     8161 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     8221 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     8281 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     8341 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     8401 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     8461 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     8521 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     8581 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     8641 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     8701 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     8761 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     8821 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     8881 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     8941 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     9001 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     9061 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     9121 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     9181 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     9241 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     9301 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     9361 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     9421 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     9481 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     9541 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     9601 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     9661 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     9721 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     9781 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
     9841 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
     9901 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
     9961 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    10021 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    10081 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    10141 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    10201 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    10261 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    10321 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    10381 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    10441 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    10501 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    10561 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    10621 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    10681 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    10741 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    10801 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    10861 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    10921 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    10981 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    11041 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    11101 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    11161 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    11221 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    11281 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    11341 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    11401 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    11461 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    11521 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    11581 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    11641 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    11701 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    11761 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    11821 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    11881 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    11941 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    12001 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    12061 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    12121 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    12181 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    12241 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    12301 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    12361 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    12421 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    12481 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    12541 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    12601 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    12661 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    12721 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    12781 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    12841 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    12901 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    12961 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    13021 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    13081 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    13141 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    13201 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    13261 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    13321 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    13381 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    13441 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    13501 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    13561 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    13621 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    13681 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    13741 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    13801 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    13861 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    13921 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    13981 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    14041 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    14101 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    14161 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    14221 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    14281 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    14341 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    14401 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    14461 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    14521 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    14581 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    14641 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    14701 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    14761 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    14821 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    14881 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    14941 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    15001 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    15061 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    15121 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    15181 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    15241 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    15301 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    15361 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    15421 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    15481 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    15541 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    15601 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    15661 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    15721 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    15781 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    15841 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    15901 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    15961 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    16021 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    16081 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    16141 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    16201 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    16261 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    16321 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    16381 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    16441 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    16501 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    16561 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    16621 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    16681 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    16741 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    16801 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    16861 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    16921 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    16981 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    17041 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    17101 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    17161 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    17221 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    17281 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    17341 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    17401 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    17461 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    17521 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    17581 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    17641 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    17701 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    17761 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    17821 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    17881 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    17941 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    18001 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    18061 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    18121 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    18181 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    18241 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    18301 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    18361 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    18421 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    18481 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    18541 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    18601 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    18661 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    18721 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    18781 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    18841 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    18901 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    18961 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    19021 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    19081 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    19141 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    19201 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    19261 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    19321 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    19381 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    19441 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    19501 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    19561 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    19621 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    19681 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    19741 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    19801 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    19861 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    19921 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    19981 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    20041 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    20101 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    20161 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    20221 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    20281 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    20341 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    20401 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    20461 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    20521 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    20581 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    20641 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    20701 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    20761 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    20821 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    20881 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    20941 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
    21001 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    21061 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    21121 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    21181 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    21241 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    21301 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    21361 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    21421 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    21481 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    21541 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    21601 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    21661 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    21721 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    21781 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    21841 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    21901 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    21961 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    22021 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    22081 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    22141 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    22201 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    22261 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    22321 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    22381 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    22441 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    22501 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    22561 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    22621 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    22681 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    22741 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    22801 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    22861 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    22921 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    22981 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    23041 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    23101 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    23161 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    23221 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    23281 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    23341 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    23401 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    23461 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    23521 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    23581 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    23641 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    23701 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    23761 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    23821 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    23881 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    23941 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    24001 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    24061 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    24121 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    24181 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    24241 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    24301 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    24361 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    24421 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    24481 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    24541 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    24601 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    24661 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    24721 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    24781 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    24841 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    24901 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    24961 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    25021 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    25081 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    25141 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    25201 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    25261 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    25321 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    25381 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    25441 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    25501 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    25561 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    25621 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    25681 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    25741 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    25801 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    25861 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    25921 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    25981 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    26041 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    26101 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    26161 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    26221 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    26281 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    26341 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    26401 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    26461 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    26521 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    26581 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    26641 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    26701 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    26761 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    26821 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    26881 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    26941 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    27001 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    27061 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    27121 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    27181 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    27241 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    27301 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    27361 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    27421 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    27481 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    27541 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    27601 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    27661 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    27721 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    27781 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    27841 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    27901 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    27961 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    28021 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    28081 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    28141 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    28201 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    28261 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    28321 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    28381 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    28441 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    28501 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    28561 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    28621 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    28681 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    28741 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    28801 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    28861 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    28921 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    28981 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    29041 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    29101 attaaaa
//
LOCUS       DAJSVF010000029        28602 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSVF010000029 DAJSVF010000000
VERSION     DAJSVF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.030"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            236..1876
                     /locus_tag="ON550_001297"
     CDS             236..1876
                     /locus_tag="ON550_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR3954392.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2030..3352
                     /locus_tag="ON550_001298"
     CDS             2030..3352
                     /locus_tag="ON550_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR3954393.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3414..3845)
                     /locus_tag="ON550_001299"
     CDS             complement(3414..3845)
                     /locus_tag="ON550_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954394.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3909..4745)
                     /locus_tag="ON550_001300"
     CDS             complement(3909..4745)
                     /locus_tag="ON550_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3954395.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4935..5237)
                     /locus_tag="ON550_001301"
     CDS             complement(4935..5237)
                     /locus_tag="ON550_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR3954396.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="ON550_001302"
     CDS             complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="ON550_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR3954397.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5691..5957)
                     /locus_tag="ON550_001303"
     CDS             complement(5691..5957)
                     /locus_tag="ON550_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR3954398.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6211..6936)
                     /locus_tag="ON550_001304"
     CDS             complement(6211..6936)
                     /locus_tag="ON550_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3954399.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7302..7372)
                     /locus_tag="ON550_001305"
     tRNA            complement(7302..7372)
                     /locus_tag="ON550_001305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7338..7340),aa:Gly,seq:ccc)
     gene            7489..8241
                     /locus_tag="ON550_001306"
     CDS             7489..8241
                     /locus_tag="ON550_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3954400.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8736
                     /locus_tag="ON550_001307"
     CDS             8257..8736
                     /locus_tag="ON550_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3954401.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8987..10567)
                     /locus_tag="ON550_001308"
     CDS             complement(8987..10567)
                     /locus_tag="ON550_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR3954402.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10560..11303)
                     /locus_tag="ON550_001309"
     CDS             complement(10560..11303)
                     /locus_tag="ON550_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954403.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11479..12255)
                     /locus_tag="ON550_001310"
     CDS             complement(11479..12255)
                     /locus_tag="ON550_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954404.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12503..13339)
                     /locus_tag="ON550_001311"
     CDS             complement(12503..13339)
                     /locus_tag="ON550_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR3954405.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13350..14279)
                     /locus_tag="ON550_001312"
     CDS             complement(13350..14279)
                     /locus_tag="ON550_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3954406.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14471..14824
                     /locus_tag="ON550_001313"
     CDS             14471..14824
                     /locus_tag="ON550_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3954407.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14898..15554)
                     /locus_tag="ON550_001314"
     CDS             complement(14898..15554)
                     /locus_tag="ON550_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3954408.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15780..17714
                     /locus_tag="ON550_001315"
     CDS             15780..17714
                     /locus_tag="ON550_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3954409.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17800..19023)
                     /locus_tag="ON550_001316"
     CDS             complement(17800..19023)
                     /locus_tag="ON550_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3954410.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19017..20915)
                     /locus_tag="ON550_001317"
     CDS             complement(19017..20915)
                     /locus_tag="ON550_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR3954411.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20909..22027)
                     /locus_tag="ON550_001318"
     CDS             complement(20909..22027)
                     /locus_tag="ON550_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3954412.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22110..22943)
                     /locus_tag="ON550_001319"
     CDS             complement(22110..22943)
                     /locus_tag="ON550_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3954413.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22936..23853)
                     /locus_tag="ON550_001320"
     CDS             complement(22936..23853)
                     /locus_tag="ON550_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3954414.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24080..25204)
                     /locus_tag="ON550_001321"
     CDS             complement(24080..25204)
                     /locus_tag="ON550_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3954415.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25387..26295
                     /locus_tag="ON550_001322"
     CDS             25387..26295
                     /locus_tag="ON550_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3954416.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26436..28049
                     /locus_tag="ON550_001323"
     CDS             26436..28049
                     /locus_tag="ON550_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3954417.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8891 a         5622 c         4868 g         9221 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc
     2161 tttaggaaaa ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa
     2221 tttatttcat tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc
     2281 cgtttttgcg atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt
     2341 agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc
     2401 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
     2461 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg
     2521 tatgcttatc ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc
     2581 tggtggtgtt ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac
     2641 ggacgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc
     2701 tatcattggt gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa
     2761 tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc
     2821 cattccacaa ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat
     2881 ctgtaactat tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat
     2941 ctgtaaaatc gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa
     3001 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa
     3061 tctaaatgta ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt
     3121 tatcgcgatt tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga
     3181 atcaactatt actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt
     3241 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg
     3301 tggtgccatc gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa
     3361 aatacctgat catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg
     3421 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg
     3481 tgtgcttcta ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc
     3541 gcttcttcta cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac
     3601 acagcaagtg ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct
     3661 tgtacatagc cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt
     3721 tctggctcct tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat
     3781 cctaattgtt ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc
     3841 aacatccaaa tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac
     3901 tccttttttc acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct
     3961 tctaatgttt gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca
     4021 atcgcttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4081 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4141 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4201 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4261 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     4321 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     4381 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     4441 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     4501 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     4561 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     4621 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     4681 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     4741 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     4801 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     4861 agtgctaaac gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta
     4921 tcgattcttt tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     4981 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5041 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5101 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5161 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5221 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5281 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5341 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     5401 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     5461 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     5521 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     5581 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     5641 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     5701 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     5761 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     5821 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     5881 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     5941 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6001 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6061 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6121 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6181 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6241 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6301 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6361 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     6421 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     6481 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     6541 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     6601 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     6661 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     6721 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     6781 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     6841 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     6901 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     6961 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7021 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7081 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7141 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7201 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7261 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7321 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7381 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     7441 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     7501 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     7561 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     7621 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     7681 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     7741 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     7801 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     7861 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     7921 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     7981 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8041 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8101 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8161 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8221 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8281 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8341 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     8401 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     8461 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     8521 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     8581 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     8641 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     8701 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     8761 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     8821 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     8881 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     8941 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9001 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9061 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9121 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9181 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9241 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9301 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9361 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
     9421 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
     9481 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
     9541 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
     9601 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
     9661 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
     9721 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
     9781 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
     9841 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     9901 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     9961 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10021 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10081 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10141 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10201 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10261 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10321 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10381 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    10441 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    10501 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    10561 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    10621 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    10681 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    10741 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    10801 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    10861 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    10921 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    10981 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11041 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11101 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11161 actaatcttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11221 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11281 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11341 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    11401 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    11461 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    11521 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    11581 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    11641 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    11701 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    11761 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    11821 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    11881 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    11941 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    12001 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    12061 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    12121 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    12181 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    12241 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    12301 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    12361 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    12421 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    12481 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    12541 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    12601 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    12661 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    12721 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    12781 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    12841 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    12901 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    12961 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    13021 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    13081 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    13141 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    13201 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    13261 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    13321 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    13381 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    13441 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    13501 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    13561 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    13621 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    13681 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    13741 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    13801 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    13861 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    13921 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    13981 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    14041 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    14101 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    14161 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    14221 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    14281 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    14341 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    14401 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    14461 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    14521 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    14581 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    14641 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    14701 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    14761 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    14821 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    14881 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    14941 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    15001 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    15061 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    15121 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    15181 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    15241 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    15301 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    15361 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    15421 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    15481 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    15541 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    15601 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    15661 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    15721 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    15781 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    15841 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    15901 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    15961 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    16021 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    16081 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    16141 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    16201 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    16261 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    16321 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
    16381 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    16441 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    16501 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    16561 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    16621 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    16681 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    16741 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    16801 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    16861 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    16921 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    16981 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    17041 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    17101 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    17161 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    17221 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    17281 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    17341 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    17401 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    17461 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    17521 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    17581 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    17641 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    17701 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    17761 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    17821 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    17881 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    17941 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    18001 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    18061 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    18121 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    18181 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    18241 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    18301 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    18361 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    18421 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    18481 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    18541 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    18601 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    18661 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    18721 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    18781 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    18841 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    18901 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    18961 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    19021 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    19081 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    19141 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    19201 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    19261 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    19321 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    19381 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    19441 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    19501 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    19561 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    19621 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    19681 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    19741 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    19801 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    19861 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    19921 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    19981 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    20041 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    20101 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    20161 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    20221 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    20281 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    20341 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    20401 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    20461 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    20521 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    20581 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    20641 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    20701 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    20761 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    20821 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    20881 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    20941 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    21001 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    21061 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    21121 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    21181 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    21241 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    21301 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    21361 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    21421 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    21481 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    21541 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    21601 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    21661 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    21721 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    21781 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    21841 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    21901 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    21961 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    22021 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    22081 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    22141 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    22201 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    22261 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    22321 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    22381 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    22441 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    22501 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    22561 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    22621 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    22681 ctggctgtag ctaaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    22741 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    22801 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    22861 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    22921 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    22981 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    23041 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    23101 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    23161 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    23221 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    23281 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    23341 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    23401 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    23461 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    23521 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    23581 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    23641 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    23701 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    23761 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    23821 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    23881 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    23941 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    24001 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    24061 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    24121 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    24181 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    24241 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    24301 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    24361 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    24421 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    24481 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    24541 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    24601 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    24661 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    24721 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    24781 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    24841 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    24901 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    24961 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    25021 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    25081 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    25141 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    25201 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtgtat
    25261 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    25321 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    25381 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    25441 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    25501 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    25561 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    25621 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    25681 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    25741 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    25801 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    25861 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    25921 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    25981 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    26041 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    26101 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    26161 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    26221 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    26281 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    26341 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    26401 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    26461 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    26521 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    26581 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    26641 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    26701 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    26761 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    26821 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    26881 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    26941 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    27001 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    27061 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    27121 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    27181 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    27241 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    27301 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    27361 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    27421 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    27481 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    27541 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    27601 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    27661 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    27721 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    27781 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    27841 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    27901 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    27961 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    28021 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    28081 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    28141 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    28201 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    28261 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    28321 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    28381 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    28441 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    28501 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    28561 tgtattaaat aatttaattt ttttgttcct atttatttaa ca
//
LOCUS       DAJSVF010000030        26666 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSVF010000030 DAJSVF010000000
VERSION     DAJSVF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.031"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(33..1265)
                     /locus_tag="ON550_001324"
     CDS             complement(33..1265)
                     /locus_tag="ON550_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3954418.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1519..2088
                     /locus_tag="ON550_001325"
     CDS             1519..2088
                     /locus_tag="ON550_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3954419.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2266..2706)
                     /locus_tag="ON550_001326"
     CDS             complement(2266..2706)
                     /locus_tag="ON550_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR3954420.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2864..3628
                     /gene="map"
                     /locus_tag="ON550_001327"
     CDS             2864..3628
                     /gene="map"
                     /locus_tag="ON550_001327"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR3954421.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3660..4583
                     /locus_tag="ON550_001328"
     CDS             3660..4583
                     /locus_tag="ON550_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR3954422.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4659..5798
                     /locus_tag="ON550_001329"
     CDS             4659..5798
                     /locus_tag="ON550_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR3954423.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5791..6591
                     /locus_tag="ON550_001330"
     CDS             5791..6591
                     /locus_tag="ON550_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3954424.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6591..7418
                     /locus_tag="ON550_001331"
     CDS             6591..7418
                     /locus_tag="ON550_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3954425.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7396..8130
                     /locus_tag="ON550_001332"
     CDS             7396..8130
                     /locus_tag="ON550_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3954426.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8230..9096
                     /gene="rfbA"
                     /locus_tag="ON550_001333"
     CDS             8230..9096
                     /gene="rfbA"
                     /locus_tag="ON550_001333"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR3954427.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9110..9682
                     /gene="rfbC"
                     /locus_tag="ON550_001334"
     CDS             9110..9682
                     /gene="rfbC"
                     /locus_tag="ON550_001334"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR3954428.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9704..10732
                     /gene="rfbB"
                     /locus_tag="ON550_001335"
     CDS             9704..10732
                     /gene="rfbB"
                     /locus_tag="ON550_001335"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR3954429.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10830..11681
                     /gene="rfbD"
                     /locus_tag="ON550_001336"
     CDS             10830..11681
                     /gene="rfbD"
                     /locus_tag="ON550_001336"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR3954430.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11715..13748
                     /locus_tag="ON550_001337"
     CDS             11715..13748
                     /locus_tag="ON550_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954431.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13792..15057
                     /locus_tag="ON550_001338"
     CDS             13792..15057
                     /locus_tag="ON550_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3954432.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNHNSYNSILNIYGLIGFGIAALLLILFIGRLTLKAD
                     LSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEEFYVME
                     EAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15267..16073
                     /locus_tag="ON550_001339"
     CDS             15267..16073
                     /locus_tag="ON550_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954433.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16085..17305
                     /locus_tag="ON550_001340"
     CDS             16085..17305
                     /locus_tag="ON550_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954434.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17295..18881
                     /locus_tag="ON550_001341"
     CDS             17295..18881
                     /locus_tag="ON550_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3954435.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18920..21058
                     /locus_tag="ON550_001342"
     CDS             18920..21058
                     /locus_tag="ON550_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3954436.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21426..22409)
                     /locus_tag="ON550_001343"
     CDS             complement(21426..22409)
                     /locus_tag="ON550_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR3954437.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22787..24178
                     /locus_tag="ON550_001344"
     CDS             22787..24178
                     /locus_tag="ON550_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3954438.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24194..25234
                     /locus_tag="ON550_001345"
     CDS             24194..25234
                     /locus_tag="ON550_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3954439.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25253..26338
                     /locus_tag="ON550_001346"
     CDS             25253..26338
                     /locus_tag="ON550_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR3954440.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26371..>26666
                     /locus_tag="ON550_001347"
     CDS             26371..>26666
                     /locus_tag="ON550_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3954441.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYP"
BASE COUNT         8547 a         4436 c         5417 g         8266 t
ORIGIN      
        1 atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt tcggaaaatc
       61 ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga tcctaccatg
      121 aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc atcggacatt
      181 tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg attcgatgcg
      241 ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg tgaagggtca
      301 ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag cagttttgct
      361 aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt aaaagtaaat
      421 ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga aatgtaacca
      481 tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt tgccatgaat
      541 ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt aggcaatccg
      601 ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc tcgattcaat
      661 tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac agggtcttct
      721 tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg tttttcttct
      781 ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac gacagtccaa
      841 ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc attcgctgat
      901 tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc ggcagaaaca
      961 acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat tttagattgt
     1021 ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg gatctggtcg
     1081 tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg tgtgatcaat
     1141 cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc tttttctgtg
     1201 gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt aaaatcagat
     1261 aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg ataccattta
     1321 attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt taaggttaag
     1381 tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa aaactaattt
     1441 tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt tataaaaaaa
     1501 ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca gattttggca
     1561 gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag aaacatcgcg
     1621 aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat ggatgacttg
     1681 agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt cactgttgag
     1741 catacaggga atacgattat cgatcttgct tttaattata tccattttgc aagaagagag
     1801 aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga gatgcagaat
     1861 ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt gaatctttca
     1921 gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg gatcgcagct
     1981 ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat gatccaagac
     2041 agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat atactcaaaa
     2101 gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt cgtttttctt
     2161 acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga ataaaagagc
     2221 catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa tttttgcgac
     2281 tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat tcagatccac
     2341 tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat cgaaatcatc
     2401 caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac cgtaaatttt
     2461 tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat caggcagatc
     2521 gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga attcatctgc
     2581 atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa aagcttctgc
     2641 aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga tttttgctaa
     2701 agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata ccaaaacatc
     2761 attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta aatgtatgcc
     2821 gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta ctttaaaatc
     2881 agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg tacataaaaa
     2941 tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat ttgtcagaaa
     3001 ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt atgaatatgc
     3061 gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa agcctttgaa
     3121 aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca tttctgattc
     3181 ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga tggaagtaac
     3241 gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga ttggtgacat
     3301 cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc gtgattttat
     3361 tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt acggagaagc
     3421 tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta tggttaatac
     3481 cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa gagacggcgg
     3541 actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa gaattttgac
     3601 atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg gagaggtaca
     3661 tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt gagacgactc
     3721 aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct tattatttac
     3781 tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt ctccacatcg
     3841 atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt taccagttta
     3901 tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta tccgtttctg
     3961 cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt gcgatgaata
     4021 aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc tcactaattg
     4081 tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg ttgggacagc
     4141 tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc gatcagttcc
     4201 aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc ttgatttatt
     4261 taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg atcttcgcta
     4321 ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat tttgcaacga
     4381 ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg cttaattttg
     4441 ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa tacgtgacgg
     4501 gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa ttgaaaacta
     4561 aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat atcagtaatt
     4621 tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct tttagtatgg
     4681 ttataagatt ttttctgaca attttattat cactgatatt aacacctgta ttcaaaatca
     4741 tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat aaagttccaa
     4801 tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact ttattcttat
     4861 tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct ttagtaatca
     4921 tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa agtataggga
     4981 tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat tccttcacgc
     5041 ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg attttttgga
     5101 tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg gcggcaggga
     5161 tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc catgcttcta
     5221 cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt ttttttcctt
     5281 acaattttta tccggcaaag atttatttag gcgatacagg agctctattc ctagggttta
     5341 tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca tcgatttctt
     5401 tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt aggagaaaag
     5461 caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga ctaatcagtt
     5521 taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg atgttttctt
     5581 tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg attattgcca
     5641 tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag aaacatcagt
     5701 ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa gtaaaacaga
     5761 aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca gccgatcctt
     5821 attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa ttttatttca
     5881 actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc acttcccgag
     5941 tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta ttttcatgat
     6001 gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga ttctcgctat
     6061 ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc attggtcttg
     6121 ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa tgaagatgga
     6181 acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt gcgctttgtg
     6241 cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga atgccgagga
     6301 ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat ggtagctgat
     6361 attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta ctttgaagac
     6421 aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac acccttttat
     6481 gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt
     6541 ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg atgaaaccag
     6601 aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca gcttatggac
     6661 cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat aatagtcctc
     6721 aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa
     6781 atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa ggaacacgtt
     6841 gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa ctattgcgta
     6901 aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct gggagccgac
     6961 aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca gtagaagttg
     7021 aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat
     7081 ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg gatcattggc
     7141 tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac
     7201 atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt tctattttac
     7261 aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt caccgacgac
     7321 aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat atctggcgga
     7381 tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata
     7441 gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt atctcagctt
     7501 tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac atgggatata
     7561 ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc tcgtcaagga
     7621 ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat ctttttggca
     7681 gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag
     7741 aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga gctgcaagag
     7801 atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa taatgtgata
     7861 agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc cgatattttg
     7921 ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg aggaaagaag
     7981 actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa cgcgagtgag
     8041 attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct gattacggcg
     8101 ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt aatatttcca
     8161 tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca aaaaggagag
     8221 cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct gtacccttta
     8281 acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat ttattatcca
     8341 atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac gccacaagat
     8401 acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca gatcgaatat
     8461 gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga agaatttatt
     8521 ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg cggtctgtct
     8581 aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc
     8641 aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt
     8701 gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta tttttatgac
     8761 aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga actagagatt
     8821 acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact tatgggacgc
     8881 ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc tacctttatc
     8941 gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt
     9001 atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa aaaaaatcaa
     9061 tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga tgaaagtaac
     9121 agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg
     9181 tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga aatatgactt
     9241 tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc ttcatttcca
     9301 aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag ttttagatgt
     9361 agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat ttatcttatc
     9421 tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat tcgttacttt
     9481 gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag aagcagatgg
     9541 gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc catcaagagc
     9601 gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag cagaaaatcc
     9661 atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa acatcattgt
     9721 taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa acaatcatcc
     9781 agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag agaacttggc
     9841 tggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg cagaattagt
     9901 ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat cccataacga
     9961 taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta cgtatacact
    10021 tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg acgaggttta
    10081 cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag gagaaaaatt
    10141 cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag ctggttcaga
    10201 tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt caaactgttc
    10261 aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag taacgaatat
    10321 cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg actggatcca
    10381 tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa ttggcgaaac
    10441 ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat tgatcttaga
    10501 aatgatgggt caaccaaaag atgcgtacga ccatgtaaac gaccgtgcag gacacgactt
    10561 gcgttatgca atcgattcga caaaattacg ggaagagtta ggatggaagc ctgagtttac
    10621 gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg aagactggtg
    10681 gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat aatacagaga
    10741 aaatgtcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg tctctttttt
    10801 aaaataaaaa tcaaggagcg gataatcata tgtttttatt aactggagga aatgggcaac
    10861 taggtacaga attgcgccat ttattagatg aaaaaaatct gcaatatgtt tcaacagatg
    10921 caaaagactt ggatatcaca gatgccgaga aaacaatggc ttacatcaca gaattaaaac
    10981 ctgatgttat tttccattgt gcagcttaca cagcagtaga taaagcagaa gaagaagcaa
    11041 aagaattaga tgagaagatc aatgttgatg gtacaagaaa tgtagcactt gcagcaaaag
    11101 ctgtgaatgc tcaattcgtc tatatcagta cagactatgt tttcgatgga aaaaacaaaa
    11161 atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt cgtacgaaat
    11221 tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata cgaacttctt
    11281 gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta gcggaaaccc
    11341 gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca cgtactttgg
    11401 cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat catttgtcga
    11461 acgaaaatag ctgttcttgg tatgaattcg caaaagaaat attgaaagat acagatgtag
    11521 aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg cagtattcag
    11581 tcatgagcct gaaaaagaca gaagagttag gatttgtcat accaacttgg caagaagcac
    11641 ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt atcaaataga
    11701 atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa aagccaaaag
    11761 cgaaccaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc tatcttattg
    11821 gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca cttggtgttt
    11881 gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt tttggacaga
    11941 atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc tcacaagctg
    12001 aagaggactt tccgttggtg aacgaggatt attcattaaa cggacaaaac atgatcattg
    12061 cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac tggggattct
    12121 ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta gtcgtcatgg
    12181 tcttgcttgg attcgaagta ctgatgatat taacgaagag aaaaaaagct ctatcgctaa
    12241 tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg caacatgtgg
    12301 gcgatttgat tttctttact ttgggactga tgactgcttt ttatcattat ttctacaatc
    12361 atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc ggtattgggt
    12421 ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg attttttatt
    12481 ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg gatgtagtgt
    12541 tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg ctaacgtcaa
    12601 aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta agtactggag
    12661 gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt ccttttgaag
    12721 atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt ccaactgtat
    12781 tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag ttactgggac
    12841 gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg ttgccaaaat
    12901 ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc cttactttta
    12961 gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga gaagatgtta
    13021 ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat ttctatgcga
    13081 ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca gttgctgtgt
    13141 tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt tacctcgctt
    13201 ttgccgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa ggtacaggag
    13261 cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa gatcctggta
    13321 aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga gtgaaatcct
    13381 tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat ccgaaagaag
    13441 aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct aaggaagata
    13501 caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt gaagacgcag
    13561 aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac aaccaattat
    13621 atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg atttttaaag
    13681 tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat cagctagtcg
    13741 tacagtaata ggctagctga gaataatcct gaaatgttag tagaaaggag agtggagata
    13801 attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt gcttttgtta
    13861 ctgacgctag tcttttttgt ttggacagca gggttaggtt ttccagcaac tttgtttata
    13921 tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat tttaaatgtt
    13981 tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct tttcattttc
    14041 ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact gtttcctttg
    14101 attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga tcgcggtatt
    14161 tatctgattg tcttttcggt cttcctcgga atcacgttat atcgtgtatt tacagagatc
    14221 caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga tatttggatc
    14281 aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag tggatttgcc
    14341 agttcttttg aacgatggta tgtacgaatc atcggtgcag tagcagttgt tgcagctttt
    14401 ctcgggattt gggtctgcca atctagaggt gcattagtcg cgttgattgt ttttgcgata
    14461 ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat tgcttacacg
    14521 gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga aaaagtcaat
    14581 ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg agaaaaatct
    14641 gaacaaattt tactaggaat gaaaacattt atcttccaac gaggaaacca caatagttat
    14701 aattctattt tgaatatcta tggattgata ggatttggga ttgctgcttt gctgcttatc
    14761 ctttttattg gtcgcttaac gttgaaagca gatttatcca acgggcagat gacttttatc
    14821 tgggcctttt ttgcggtgat gatgcagtca tttatggagg atacattgac ttcataccca
    14881 tgggttccga ttatttatat cctgttggct atggccgttc atcgatatga tgaaccagaa
    14941 gttgcggagg aattctatgt tatggaagag gcatatgacg atatgactta tgatgataca
    15001 tcttatgagg aacaaccaga tagcgctcat tcttccagga tccaacgcta tcattgatag
    15061 gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct tttttggttt attgcaattg
    15121 tctagcatct tctgtaaaat agaaacgtta gagaaaatct tgtatcatag aaaattaaga
    15181 aagaaataag aacgcagctc ggtaccaaaa atatggtaaa atggcacttg actgtaattc
    15241 actgaaaatc gcttggagga acacaaatgt ttaaagattt gtatttattt atgaaggaca
    15301 tttatcagaa cagaaaatta ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg
    15361 caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc gctatttact gtattgattt
    15421 attggctggt atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa tacccattta
    15481 tcgtatattt agttaccggg atgattccct ggttcttctt ctcagatgca tttgcatcaa
    15541 ctacggttgt ttttcgggaa tatacttatt tagtaaagaa agtcgtattc aatattcgta
    15601 tcttgcccac gacgaaaatc ctatccaatc tgtacaccca tttattcttc attctgattg
    15661 gcatagctgt cgcgcttata aatggtatat atccttcagc aatgtcttta cagctgattt
    15721 attatttatt ttgcatgtgt gctttcttga caggattgac ttggctgacg gcatcgatcc
    15781 aaccattttt acctgatatc atgcaattaa ttacgatcgt gctgcagttg atcatgtgga
    15841 cattaccgat attatggagc cctaatcagt ttgattcagg tcttgtaagc atattgaaat
    15901 taaatccgct gtactatgtt gttcaaggtt atcgtgagtc atttttaagt gaagcttggt
    15961 tctgggagca tggaacatat actttgtatt tctgggtttt cacgttagta atgttcgtaa
    16021 tcggctctgc agtgttccgt agattgaaac cacatttttc agatgtgctg taaggagaat
    16081 agctatgaca gaatatgcaa ttagactaaa agatatcaca aaatcttata atatgtacgc
    16141 aaaaccttcg gatcgatttc gtgaagccct caatcctttt aagaaatcct atcatgatgt
    16201 attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt
    16261 gggagaaaac ggatcgggaa aatctacaat gttgaaaatc attacgggag tactgacacc
    16321 aacatccggc acgatggaaa tcgaaggaaa aatctcagct ttgctagaac taggatcagg
    16381 attcaatcct gagtattctg gctatgaaaa tatctattta aatggaatgg ttctaggatt
    16441 ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt gaatttgctg acatcggaga
    16501 ccatatttat caacctgtaa aaacttattc aagcgggatg ttcgttcgtc tagcttttgc
    16561 tgtagcaatc aatgtgaatc ccgatatttt gattgtggat gaagcattgg cagtaggcga
    16621 tcttgaattt caactaaaat gtatggaaaa atttacagaa atcaagaact cgggaaagac
    16681 gattttattc gtttctcatg atatcagttc gattcgtcgt ttttgtgatc gttcctattg
    16741 gttgaaaaac ggagaagtcg tcgaatatgg cgatacgatg gaagtaacga cgaattatga
    16801 aaacttttta aagaaaaaat cagttaaaac agtggatcgg aataaaaacc aagtagaacg
    16861 gttcgccgct ccagatatcg tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa
    16921 tccggtggat atgctggaac aaggagaaaa actatacgtt aaagtaactt atgaagtaaa
    16981 agatgataca atcaaaaatc cggttttagg aattgcgcta cgaacagtcg acaattttta
    17041 tgtatgcgga cttaatactc tactagatgg tatcaagatt ccttggaaaa aaggagagaa
    17101 tatcttttat ctggagtatg aaaagttagg tttgttagct ggtgaatatt attttgacgt
    17161 tgcggtcttt gaagaaaatg caacggttcc tttggtatat aagacaaagt acatgaactt
    17221 gtttgtcagc ggttcttata tcggagaagg catcgtggtc ttagaccata aatgggaaga
    17281 agggactcat agcaatgaaa tatgattttg aaatggacct agatgaacaa agcagtgtgg
    17341 gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt agagtttggt cccggaaacg
    17401 gccgattaac gaaacattta atcggagcaa aacaatgtga agtgagcatt gttgagttag
    17461 ataaggaatt gttcgatttt gtcagcgaat ttgctcaaga tggattttat ggtgatatcg
    17521 aaagttttga gtgggcaaac tattatgctg gacagacatt tgattacgtc ttgtttgcgg
    17581 atgttttaga acatttggta gatcctggaa aaacattgaa gaaagtcaga gaatttttga
    17641 atgaagaagg agagatcctg attacattcc ctaacttggc gcataattct gtcatgatcg
    17701 atttgttcaa taaccaattg ccgtgggctt cttatggtct cctagatgaa acccataatt
    17761 ctttttatac tcatgatggc ttccaaaaag tttttgaaaa agcgggatta ttcattaata
    17821 ttgaagatta tttgtactta gctgtaggtg atacagaatt aaaatcaact tatgaagaat
    17881 tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc taccaatact
    17941 ttttctcatt gatgaaacat ccagtagcac aaagttccat tgcagagcca caaaattcta
    18001 attatgtaaa agtgttagaa gtaacccagc aaacgaaaca agatgaaaca atccaacaaa
    18061 taccattcaa taatttcaca ggggaaaatg agacactaag tttccccgta tcagaaacaa
    18121 cagaaagaat ggtgtttcgc ttcgcgcaac aacctagctt tatcgaattc agcgcagaag
    18181 ctgccggaga aaaactaaca tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt
    18241 gttatttgtt tgatgggaaa gaattaccag agttcgttct aacggatatt tctgggaaag
    18301 aagtgactat ccattgtcac tatcgcttta tcggggaatt gacaccaaca atgaaagaat
    18361 tgttggaaac gattcgtccc atggctgaag tcacaaagca gctttctgag aaaaatgacg
    18421 aattagaaag agaaaatcat catcttcttg aggaaaatac aagactagac catacgttaa
    18481 aaacaaccac aaatcgctat tgcacattgt tggaaagtga tgaatggaca ataaaacata
    18541 gactaggacg acgtaagaaa gaaacttcga aaaaaatcca agaaaaagaa ctttctatat
    18601 gtattgacga gaaaatctgg gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa
    18661 tcgctaattc ggatcgccag cccttgtcct ataagttatc agctgaccaa tcgccatttt
    18721 tcgaagcaat tcctgtttca cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa
    18781 cagaagcggg atttgagtta cgaatccttt gcgagcaaga acgttcattc ttagtggaag
    18841 cagtagctca aaatggccaa tcatggatca ttgaaatgta aataatgatt aacttataaa
    18901 gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac
    18961 tcgtgaacgg gccactggcg atttgcttat tgtgggctgg gcaattgatg aagtgaccaa
    19021 agaaatccct acgataaagg tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt
    19081 aagattggat atcaatcatt tatataatct tgatgttaag acccaaagcg gttttaaggt
    19141 acgtttatca gggaaaatga gaggaaaagc catcttagac ttccaaacag cgagacatca
    19201 aaatggcata gctgtgaaat taaacggtaa atacccttat gatgacggca ttgaatcttc
    19261 atgggaaaga aaaaaaagat tactgaaaaa agggataaat tatgcaagga ctcatggcgt
    19321 aaaaaaagcg atccgtcggg tcaaattgga attgaagcca ggatcgatcg actatgcaga
    19381 gtggatcagt cgtcatgaga aaccagattt gaaagagcaa aggaaagaag cacaaggatt
    19441 tgcgtaccgt ccattgattt ccatacttat gcctgtctat aacgttgaaa tcaaatggct
    19501 cgaaaaatgc attgattcgg tactggatca aacgtatgat cattgggaac tatgtatcag
    19561 tgatgatgct tctactgatc cagctatccg aaagtgtctg gaatcctatc aagcaaaaga
    19621 tgatagaatc aaagtcgttt tccgaaaaga aaatggacac atcagtctgg caactaattc
    19681 tgcattggaa atagcagaag gagaatttat agcactctta gataatgatg atgaattacc
    19741 gccatttgca ttatatgaag tggcaaaagt gctaaacgta catccagaac tggatgtgat
    19801 ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc
    19861 agactggtcg cctgatacat tgatgggaaa taattatatc tctcacttag gcgtatatcg
    19921 aacaagcatt gttaaagaac ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta
    19981 tgatttggtc cttcgtgtga cagaacaaat ccctgcagat catatttacc acattgaccg
    20041 agtcttgtat cattggcgga cgatcccagg atcaactgca agtaatggcg aagccaaaag
    20101 ttatatctat gattccggag tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa
    20161 aggaagcgta cgtcctggca gaatttctgg attttatgag attgcttatg acgtcttgca
    20221 agaggatttg gtcagtgtga tcattccaac gaagaatggc tatgaagatc taaaaacttg
    20281 tgtggattcg atcatcgaaa aaacgtctta ttcaaattat gaaatcatta ttgcggataa
    20341 cggaagtaca gatccaaaaa tgcaagaatt atttgcagaa tacaagcatc agctgaaaga
    20401 ccgctttatc gtagaattga ttgacattcc attcaattat tctcgaatta acaatttagc
    20461 agcagaaaaa gcaaacggca aatacttttt attcttgaat aacgatacag aagtgattga
    20521 gccagattgg atgacggtga tggtttctta tgcacagttt gatcgaatcg gctgtgtagg
    20581 agcaaaactg ttttatccag atgatacaac gcagcatgca ggagtattat taggaatcgg
    20641 tggtgttgca gggcatgcat tgaataacta tgatcgaaca cattgtggtt atttcggccg
    20701 attggtcatt gacgtcaatt acttagcagt cactgcagct tgtatgatgg tcaaggcagc
    20761 tgacttcaat gctgttaacg ggtttgatga aacattagaa gtagcattca acgatgttga
    20821 cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc tatgcacatc aagcagaatt
    20881 gtaccatttc gaatctaaat caagaggtta tgaagatact ccagaaaaac aaagacgttt
    20941 tgctggtgaa atcaaaaaaa tgcaagataa atggccagca tatattgcgc atgatccatt
    21001 ttacaatgat aatttaacaa aagaaggaat cggcgacttt tcgttgagac cagattaaac
    21061 tataataaaa agctgttatg ttgtgattcg ctcgcacata acggcttttt atagtttttt
    21121 gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt cgaggttggg acagaagtgt
    21181 ttaactccga gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc
    21241 ggcttattta agaagaagtt gcttctgctc ccaccgttta ttcgttttta aagaatgaga
    21301 caaaagtgtt tcttactttt gtcccacgct cttattcatg ctcagagcgc caatagatac
    21361 aaaatcatag ccaaagtttc acgaacccta gctgtcaaag aatcgatatt ttattttaat
    21421 ataggctatt ttaaaataaa tggtttaagg tattcctcta caaaagccac tctatcaatt
    21481 atcatgggtt taaaataaga agtgatggag ataaccagat tgttcttagc gtcaatataa
    21541 agaacatttc cactattacc aattgctgaa tatatttcgt tctcagaatc taatacccac
    21601 cataaatagc catatctcat attttgtaag tcttctaaag cactggttct aggtttgaac
    21661 atctcgttta tccacatggg tggtacgatt tgttttcctt tatacatccc tttatttagg
    21721 cataatacac caatttttgc cagatcactt gccgataaac acaaaccata tcctgccgtc
    21781 ccgatattta gtggatcaca aaaccagaca ttggttttag gagacttatc cattgtaaac
    21841 tgcttatgtt cttcagctgt ttcagcataa taattgacat ggtttggtat ccctatagga
    21901 tcaaatagat acttattggc aaaatcaaca gttgttagct tacttgtctg atacaacaag
    21961 ccagttaata tgtgtaaaca gacagtttga tagttgaatt cgccggttat accttttctt
    22021 cctcccaata aatctagtga tgtttttgtc caatcttcac ttgaacagac ttttgtccat
    22081 ggatcgcctt tacatttgta gggtgctttc attgttagca agtgcttgat tgtaatagcg
    22141 taaatagttt tctccccgcg tttcacttta tactctggaa aataatctaa tactttatca
    22201 tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt
    22261 ttcgttgctg acatgatatg acaagtatcg tcttttttat aaccattcca ttcatctgta
    22321 taaacttcta ccccatcctt atatgcgaca atttgacata tatttggttg tcgaaaacca
    22381 ataaaatcat gcaactcttg tttattcata agatattacc tcactttctt ctataagcat
    22441 aatgtcatta aaaatcgatt tcaatttttt cgattcttca cgtaatacag gtattcttat
    22501 ggactagaca gcgctttggg aagatggatt ctctgaaaaa tataaaggtt cactttatga
    22561 gtggataaaa aaagcagcaa atacttcgac ttctttaatt taccaacatt agtgaaaatc
    22621 gattcatctg gagatttgta atttagagtc cttgtcttga ttagaggtgt ataaagcttt
    22681 ttattagaca tttttaaatt ccattacaat ttgcttataa actaggtttt taaaggtgac
    22741 tatggtaaga taatagagat attattttgt gattaggagt gtatatatga ataaaaatgg
    22801 agaatggagt gctgcacgac ggatattcat tatattaatg gatgttattg tatacaatgc
    22861 agcaatatat agtagtttct tactgaagtt tcgtggagag atcccattac gaaatttaga
    22921 aactttccaa cactcagcaa ttattatttc agtgattttt attgcgttga acattttgtt
    22981 gggtgcatat gttttttata atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg
    23041 tcaggtattg atgacactag ggatcatggt agtgactttt gcgggccgct ggtttgcttt
    23101 tccacgtctt gttatcttac tttcatttgt tctcggtacg cttttcttaa gtatctatcg
    23161 tattctcgta tataaacttt atttacgcgt aagtcgagat aaaaaggtcg tcattattgg
    23221 gttagaaaag gatgtcgcac cagcaatcca aaattttggt tcaaagaaaa acaacaaaca
    23281 taaagtcgaa gcagtcattc ttgataacta ttatgcaaac atcaaaaata tgattaaaga
    23341 catcgatatt gtctatcttg cttctcatat cgaagaaact gaaaaaatca aaatctatca
    23401 attagttact aaaaatgaaa aaaaattgtt tttgaataca acatttgaaa acttgacatt
    23461 gatcaatcca aatattatga atttcgaaga tgaaagtatt attgaagcat ctggatttag
    23521 aatccaacct gaatatgaac tattcaaacg tttgtttgat atcatcgttt cacttgtttt
    23581 attagttatt gcttcaccat ttatgttatt cacagcaatt ttagtcaaag tgacatcacc
    23641 gggaccaatc atctataaac aagtgcggat cacaaaaaat caaaaggaat tttcaatcta
    23701 taaattccgc tcgatgaccg ccacagccga agcaaaatct ggaccagttt tagctaagag
    23761 caatgatgca cgagtaacgc ctgtaggtaa atttattcga gctgtccgct ttgatgaatt
    23821 accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg
    23881 tcctttcttc gtcgatcagt tcaatgcaga aaacccttat tactacttac ggcataatgt
    23941 tcgtgctggt atcactgggt atgcacaagt ctatggaaaa tatgtctctg attacaacag
    24001 taaattacgt tttgatttac tttatatcaa aaaatattca ttgatgcttg atttaaagat
    24061 cttgctgcaa accatcaaga tcctatttga taaaatgtcc tctcaaggac ttgaagaaga
    24121 cgaaaatgtg atctcattgg cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt
    24181 gggaggcgta aaaatgaaca atccgaaatt cagcataatc gtaccagtat acaatgcaga
    24241 agcaactatt ggtcgaacga ttgatattct aaaagaattg gattatgaag agtacgaagt
    24301 cgttttagta aatgatggat caacagatca aacacaggaa atcattcaag aactgattga
    24361 aaatgatgaa cgttttgaat tgattactac gaccaatcaa gggcccggat tagcaagaaa
    24421 cgaaggaata agacagtcaa aaggagaata tctcttattc ttcgatgcag atgacacgcc
    24481 agtaaaaacg atcttgtcag attatgatca tttgctagaa aagagtccaa aagcggatct
    24541 tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga
    24601 aaacttagtt acagaatgtc actatctaac tcacgaagag tttataaaag acatgtatca
    24661 gttaatgaat gaacagttga tgtatgttgt atggaacaaa tgctatcgta gcgatattct
    24721 gaaaaataac caaattctat ttaaaactta taatagttgt gaggatcgaa tatttaactt
    24781 acattattat gaatactgtc atcaagtgat catgaaccca aaagtagaat atatttatga
    24841 atttgaaggt gggaagggga tcactaatca ataccgacct aacaagttcg aaacatttaa
    24901 agaattctat tcattagcaa atactgtaac agacgatatt aataaagacg gtatggcggc
    24961 gcttctttta aaagggacaa cttcagttat tttttctatt tatagtacct ctgcaagaac
    25021 ttcaaaagag aagaaagacg aagcaaagca gatattgagt gatccagtga tccgagaagc
    25081 caagcaaatt gctaagacgg attcttcagc aaaaaaaata acaaagttgc tttacaatat
    25141 gccagctcca ctatttttat ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc
    25201 agggttaatg gctgctttga agcgagtata ttagttaatg gaggaaaata taatgaaaag
    25261 aattttgcat tttcagggac gaatgggttt aggtggagca gagtctttca tgatgaattt
    25321 atatcgtaaa attgatcgta caaactatca atttgatttt ttgatttatg aagattatgc
    25381 agatgttcaa gactatcatt cagagattga aagattagga ggccgtattt ttgttgtacc
    25441 taatcctaag aaaaatattt ttaaatattt aatagaagtg aataagttac taaaaaaaga
    25501 atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc
    25561 caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc
    25621 tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc
    25681 aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt
    25741 tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt
    25801 taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg
    25861 attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat
    25921 gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc
    25981 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
    26041 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
    26101 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
    26161 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
    26221 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
    26281 ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag atcattaaaa
    26341 atgttcaaga tattttagga gtgattattt ttgaaaatat gttatttgat attggcatac
    26401 aatgatattg gcaacttaaa gagattggtc aatcgattga acgaaaatgc agtttttatt
    26461 attcatatag ataaaaaaac tgatatagaa ccatttattg aagcatttaa atcatatgat
    26521 aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt attggggtgg tttttccatt
    26581 gtacaagcag aaatcaattt agtaaagaga gcattacaaa atgagaaata tttaaaatat
    26641 gtattattat ctggtgctga ttatcc
//
LOCUS       DAJSVF010000031        26130 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSVF010000031 DAJSVF010000000
VERSION     DAJSVF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.032"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(33..1064)
                     /gene="queA"
                     /locus_tag="ON550_001348"
     CDS             complement(33..1064)
                     /gene="queA"
                     /locus_tag="ON550_001348"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR3954442.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKRF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1257..2432
                     /locus_tag="ON550_001349"
     CDS             1257..2432
                     /locus_tag="ON550_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3954443.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2436..3077
                     /locus_tag="ON550_001350"
     CDS             2436..3077
                     /locus_tag="ON550_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954444.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3074..3991
                     /locus_tag="ON550_001351"
     CDS             3074..3991
                     /locus_tag="ON550_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3954445.1"
                     /translation="MKRILKMTMILSSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            3988..4659
                     /locus_tag="ON550_001352"
     CDS             3988..4659
                     /locus_tag="ON550_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954446.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4971..5138)
                     /locus_tag="ON550_001353"
     CDS             complement(4971..5138)
                     /locus_tag="ON550_001353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954447.1"
                     /translation="MFLAKDEDGKFPIVEKMIEVTDTAVTSFDDAAEGVEQSRTDGKG
                     LNESHAKGCKN"
     gene            complement(5201..5689)
                     /locus_tag="ON550_001354"
     CDS             complement(5201..5689)
                     /locus_tag="ON550_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR3954448.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPKDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5890..6156)
                     /locus_tag="ON550_001355"
     CDS             complement(5890..6156)
                     /locus_tag="ON550_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3954449.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6156..6419)
                     /locus_tag="ON550_001356"
     CDS             complement(6156..6419)
                     /locus_tag="ON550_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3954450.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            6894..7079
                     /locus_tag="ON550_001357"
     CDS             6894..7079
                     /locus_tag="ON550_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3954451.1"
                     /translation="MALSYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7221..9044)
                     /locus_tag="ON550_001358"
     CDS             complement(7221..9044)
                     /locus_tag="ON550_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR3954452.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9641..12346)
                     /locus_tag="ON550_001359"
     CDS             complement(9641..12346)
                     /locus_tag="ON550_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR3954453.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12652..13599)
                     /locus_tag="ON550_001360"
     CDS             complement(12652..13599)
                     /locus_tag="ON550_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR3954454.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYHLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13789..14058
                     /gene="rpsN"
                     /locus_tag="ON550_001361"
     CDS             13789..14058
                     /gene="rpsN"
                     /locus_tag="ON550_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3954455.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14234..14596)
                     /locus_tag="ON550_001362"
     CDS             complement(14234..14596)
                     /locus_tag="ON550_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3954456.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14607..15728)
                     /locus_tag="ON550_001363"
     CDS             complement(14607..15728)
                     /locus_tag="ON550_001363"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR3954457.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15744..16094)
                     /locus_tag="ON550_001364"
     CDS             complement(15744..16094)
                     /locus_tag="ON550_001364"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR3954458.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16224..17735)
                     /locus_tag="ON550_001365"
     CDS             complement(16224..17735)
                     /locus_tag="ON550_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3954459.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18057..19409)
                     /locus_tag="ON550_001366"
     CDS             complement(18057..19409)
                     /locus_tag="ON550_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR3954460.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKDSLSMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19562..20638)
                     /locus_tag="ON550_001367"
     CDS             complement(19562..20638)
                     /locus_tag="ON550_001367"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3954461.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20834..22579
                     /locus_tag="ON550_001368"
     CDS             20834..22579
                     /locus_tag="ON550_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR3954462.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENGTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTRASQKTETVHEKEFGSFTFRASTSTEDPVAPYTVNYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTLGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22828..23295)
                     /locus_tag="ON550_001369"
     CDS             complement(22828..23295)
                     /locus_tag="ON550_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR3954463.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVIIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAIDQGLPSTEDSR"
     gene            complement(23279..24652)
                     /locus_tag="ON550_001370"
     CDS             complement(23279..24652)
                     /locus_tag="ON550_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954464.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKEIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLSWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFILAKEMGEPIHLLLITLNHESVSIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(24975..25463)
                     /locus_tag="ON550_001371"
     CDS             complement(24975..25463)
                     /locus_tag="ON550_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954465.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELVSDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25485..26087)
                     /locus_tag="ON550_001372"
     CDS             complement(25485..26087)
                     /locus_tag="ON550_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3954466.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLLFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7491 a         5568 c         4832 g         8239 t
ORIGIN      
        1 ttgttattca aaactagcgg cttttcataa ccttatttca cgaacatcgc atctccaaaa
       61 ctaaagaaac gatagcgctc ttcaatcgca tgctggtaag cagataaagt caattctctt
      121 cctgcaaagg cactaactaa catgactaaa gtcgattttg gcaaatggaa attagttgag
      181 aaagcttcta ccactttgaa ttcatagcca ggcgtaatga aaatatcagt ccatccgctg
      241 tctgctttga tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt
      301 gttccgacag ccacgatttt cccgccattt ttacgtactt ggttcaatcg ctcagccgct
      361 tcttccgtca aacggtagaa ttcactatgc atatggtgtt cttcaatttt atctacgctg
      421 acaggtcgga atgtacctaa ccctacatgt agtgtcagat agactaattc aacacccttt
      481 gcttcgatct catcaagtaa ttctttcgta aaatgcaatc cagctgttgg cgctgcagca
      541 gaaccatttt ctttcgcata gacagtttga tagcgatctg ggtcttccag acgttctttg
      601 atatatggag gcaatggcat ttctcccaat gattcaagaa tctctagaaa aattccttca
      661 tactcaaagt caatgatccg tccgccgtgt tccaattctt ctttcactac tgcttgcaat
      721 ctgccatcac caaaagagat tcttgtccca actttggcac gtttcgcagg tttgatcaat
      781 gtctcccacg tatctccttg tgtattcgtt agaagaagga cttctaaatg cgcaccagta
      841 tctggttttt ctccatataa acgagcaggc agaactcgtg tattattcat aactagcgca
      901 tcaccaggct gtaactcttc aatgatttca tggaaacgtt tatcttccat ctctccagtt
      961 ttccgatcca aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt
     1021 aattcttccg gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc actcactttc
     1081 tatcatgtgc ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc agcacttttt
     1141 cacttctatt ttttgttttc ctaaataaaa aaactaccct atttgtattt tttcattgtt
     1201 ttaggcatac tagtaataga tagttcttca ttgaaaatag aaaaagaggt ggcaccatga
     1261 ttgaatttca aaatgtgtca aaaatttata aaggtggaaa agtcgctgta gaggacgtca
     1321 atctttcatt tgaaaagggt gagttcattt gttttatcgg tacgagcgga agcggaaaaa
     1381 cgacgacgat gcggatgatc aaccgaatga ctgatccaac acaagggaaa attttgattg
     1441 atggaaaatc catccaagaa attgatccag tggaattgcg acgtcagatc ggctatgtta
     1501 tccaaagtat tggattgatg cctcacatga cgatccgtga aaatatcact cttgtccaaa
     1561 aattgttgaa agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg attgatcttg
     1621 tggaattgcc ccgtgagatg ttagatcgct acccacatga attatctggt gggcagcagc
     1681 agcgtattgg agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg atggacgagc
     1741 cattcggtgc gcttgatccc atcactcgtg attctttgca agaccttgtc aaagatttac
     1801 aagagcgctt gggaaaaacg atcgtatttg taacccatga catggatgaa gcgattaagc
     1861 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
     1921 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
     1981 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
     2041 ttacacccga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
     2101 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
     2161 ataaaaagcg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
     2221 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc ggactgaaat
     2281 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
     2341 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
     2401 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
     2461 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcgggactt gctttgatca
     2521 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
     2581 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
     2641 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
     2701 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
     2761 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
     2821 ccattgctat gccaacgatc atggctggta tccgtttagc tgccgtttac gtgattgcat
     2881 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     2941 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     3001 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     3061 aggagaatga agaatgaaac gtatattaaa aatgaccatg atactttcct cccttcttat
     3121 acttgcgagt tgttctttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     3181 cacaggtgga atcacttcag aagcacagat cttagcaagc ctagttgccg gaatggttga
     3241 acattataca gataaaaata ccgcaatcat caacaatctg gctaccacaa ctatcaacca
     3301 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     3361 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     3421 agatgaattt gagaaaagat acgatcaaac gtggttcccg tcctatggtt ttgaaaatac
     3481 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     3541 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     3601 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     3661 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     3721 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     3781 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     3841 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     3901 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     3961 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     4021 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     4081 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     4141 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     4201 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     4261 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     4321 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     4381 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     4441 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     4501 ggtcttggag atatcattat ccgaggaacc aatgcaacag atggcacatc gattatttta
     4561 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     4621 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     4681 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt ttaaggcaat
     4741 aatccactct gattcttccc tccttatggg caaagagttg ctttttttaa ctatttgtcc
     4801 attccgtata ctccaacttc atactgccaa cgggttcatc tctgcatcca ccgactaagt
     4861 ttttttctta tccgctaagg cattttgcca atccattttc ccatcactcg tcatggaatt
     4921 cataccacat gatctctaaa tactcgtctg cttgttcttt gaattgggca tcaattctta
     4981 catcctttgg cgtggctttc atttaagcct tttccatcag tcctcgattg ttcgacgcct
     5041 tcagctgcat catcaaaact ggtgactgcc gtatcggtaa cttcgatcat cttttctaca
     5101 atcgggaact tgccatcctc atccttggct aaaaacatat ctagccattc cagcttctcg
     5161 ccatgggagg tttgcggttc ctaaaaccgg tacacgattc ttatggactg catccgtcgc
     5221 aactagactt ggcgatacga tgatccttca ccagaagagc acccccgtaa accaattgat
     5281 cgatatattg ccaataagaa aagacattgg cgtaaaagat atcgatccct cgtgatgcat
     5341 tgccgttgat actgctattc atggccgaaa gcgccacgac tttgacttct tccgtttgtg
     5401 tgacattcat gttcggcaat gtcgctttgg atacccgctc tgctaaagga acgacactgg
     5461 tattgtattt cgtatctgta tcttttccaa aagaccatgc caacgaatcc tttgggaacc
     5521 aataagacga ctccggttga ttgtccattg cttttttcaa catacttttg gctgtttctc
     5581 gataaacgac atttccaatt gtcttcgcct cactttggcg ataatcgatg gtcgttgcca
     5641 ctgccaacac taacaacgat gctccaattt tactgatttt ccttgtcaca cttcttcctc
     5701 cttgttttac ataaacccgt caacattcca ttcctaaact caaacaatga cccttcaaag
     5761 gtggagatat aggaacaaat taaagaaatc atgcaataaa tccaagaagg ttggttttag
     5821 aacaaaaaaa gagtctcctt tttggggaga ctctttgtta actatctaaa aaggttggct
     5881 cttctcactt cactcataat gtgtccaggc agaataaatt tccacaactt tattttcctc
     5941 atcaactttg taaaccactc gatgctgtct attcagcctg cgtgagtatc tgccttcatg
     6001 tgtaggtctc agcttttcaa aagactgcgt gggcatatat ggatcttcct tcaatgtctg
     6061 aatcacttct tcaaattgtt tctttaaatt cgattgcttg atttttctta aatccacttt
     6121 cgcagagttt ttgattgcga ctgtatagtt actcatcaaa gagcttccca atcaatatca
     6181 tcgatattcg taaagccact gtcgtctttt tcgcggtctc gtactttatc taaagtacct
     6241 gtttgctcaa gaaaaagggt ttcttgaatc gctctccagt cttctagccc aatcaataca
     6301 gcattgtttc cactacgatc actaataatc tcgatttcag tatgattttc atttacctct
     6361 ttcaataact gataaaaatt tttacgagca gtgctaggat tcatcgttgt attagccatt
     6421 gtctctcctc ctttttctga tataattata gcgtacttga ttaagtacgt caaaaaatac
     6481 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     6541 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     6601 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     6661 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     6721 tgaataacct gaggataccc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     6781 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgcca ttcatgttca
     6841 ctatcgattt gatacttgct tagaccgtcg ggtttgtgaa tcaccaatta aaaatggcac
     6901 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gatttctact tatgaagaca
     6961 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     7021 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     7081 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     7141 tttagctggg aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     7201 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     7261 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     7321 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     7381 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     7441 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     7501 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     7561 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     7621 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     7681 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     7741 atgaagaaag gccaaatgtc ccctaaccga tagacgaaca aaatcgcaat gattgcatat
     7801 gagatgaacg cacctgtaat attggcaatc gacttgctta accatctttt tccttctttt
     7861 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     7921 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     7981 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     8041 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     8101 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     8161 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     8221 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     8281 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     8341 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     8401 aaagcgatag aaacacccgg cacagctgct cctggaagtg cagtagcttg caatggcaca
     8461 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     8521 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     8581 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     8641 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     8701 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     8761 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     8821 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     8881 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     8941 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     9001 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     9061 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     9121 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     9181 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     9241 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     9301 tttgatatct taacttttga aagagagatg taaggttgct ttctacccat cattcttact
     9361 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     9421 tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     9481 attacttgat gcagaacagc cttttcacaa cctcttggta cacggtgttc aatcagctga
     9541 ccctcggcat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
     9601 taatactcag gtcaaaacgc tgatttcaca acctcttttt ttaatgaaaa atatggctct
     9661 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
     9721 ctacgattcc aaagacaaca aacagaccga tcatgatcca gattgctttc gtagcatttg
     9781 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
     9841 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
     9901 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
     9961 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
    10021 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
    10081 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
    10141 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
    10201 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
    10261 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
    10321 tttcttttgt tgcatctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
    10381 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
    10441 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
    10501 ctgttgtcaa tgtaccggta atttcatttt ttagtgctgg ccaatcattt tggataaaat
    10561 cagcggccaa tcctaatttt tgctgaagga tcggtaattc attgttgtat aggtctgccc
    10621 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
    10681 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
    10741 tcgttacagt tgctttcgtt gaactgagaa gcgaatcaaa gtcgatctgg tccgctttgc
    10801 ttagcgcccc ttgtgcagtc ccaatcgtat cgatgacttg gtctaataga gaagtgacag
    10861 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
    10921 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
    10981 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
    11041 caataattgg ctgcaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
    11101 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
    11161 tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag
    11221 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
    11281 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
    11341 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcctgtactt
    11401 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
    11461 tcgaagcaaa atcgccgtcg atctctgcta gttgtttacc ggcattttgg atctctggga
    11521 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
    11581 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
    11641 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
    11701 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttgactt
    11761 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
    11821 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    11881 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    11941 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    12001 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    12061 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    12121 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    12181 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    12241 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    12301 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    12361 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    12421 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    12481 tgagaaataa ttaaggatta cagcctctac tttatcatga aataaaaggg aaagataatt
    12541 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    12601 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    12661 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
    12721 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
    12781 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
    12841 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
    12901 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
    12961 cgtcgcatcg attacaaggt cagcctgatc agcaaattgc agacctggat ttgttttatc
    13021 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
    13081 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
    13141 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
    13201 tagatgataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
    13261 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
    13321 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
    13381 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
    13441 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
    13501 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
    13561 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
    13621 ataagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
    13681 attcaaaaca aaatagttgt cttttttcgc gaaacgtgtt aaattattta tctaaaagcg
    13741 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
    13801 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
    13861 caggaattaa aagcgcaaaa agattacgaa ggcttgcgca aacttccttt agatgctcgt
    13921 ccagaacggt tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
    13981 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
    14041 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
    14101 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
    14161 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
    14221 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
    14281 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
    14341 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
    14401 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
    14461 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
    14521 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
    14581 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
    14641 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
    14701 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
    14761 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
    14821 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
    14881 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
    14941 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
    15001 ggataaactt ccggtagtgc gtgcccagag ggattcagtc cgtacattgc tactccataa
    15061 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
    15121 acatatcgag gaagttcagg aagttttttt agcacttcta taaagcgttc tttttgcaga
    15181 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
    15241 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
    15301 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
    15361 ttacttttct ctaaaacagc agctgctgcc tgcaaccatt cccgtgtacc tgcagttact
    15421 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
    15481 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
    15541 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
    15601 ccgtttgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
    15661 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
    15721 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
    15781 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
    15841 tgaccgtttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
    15901 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
    15961 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
    16021 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
    16081 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
    16141 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
    16201 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
    16261 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
    16321 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
    16381 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
    16441 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
    16501 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
    16561 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
    16621 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
    16681 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
    16741 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
    16801 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
    16861 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
    16921 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
    16981 acacggcgtt ttgtacgtcc gaagacgatt gttagttcag gtgtttgaac atcaaacaag
    17041 cgagtcatga tatcgaactt ttcgtattct tttgcgcgga catagtattg atcgatcaag
    17101 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
    17161 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
    17221 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
    17281 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttgatgtgg
    17341 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
    17401 tgtcggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
    17461 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
    17521 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
    17581 gttttccctg tacctgtttg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
    17641 atcgtttctg attggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
    17701 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
    17761 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
    17821 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
    17881 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
    17941 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
    18001 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
    18061 ttcatttgct gaagctgttc taccacttca aataagccca tgctgttgct gccttttagc
    18121 ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct
    18181 ttttcaaaat agaacacagg tagttggggg tatttttgat tcaattcttc ttttaatgca
    18241 tgcatttgtt ccccgtacaa gaaaatcatg gcaaatttct caggatcgat atgttcactc
    18301 atttgcgcat gcatgcttaa agaatctttc cctaattcca gcatatctgc taacacagca
    18361 attctgcgcc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
    18421 ggatttgcat tatagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
    18481 gttctgtttt gagtgataga aacatgagcg agcccttggc gtatctcagg tatggacagt
    18541 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
    18601 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
    18661 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
    18721 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctggt
    18781 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
    18841 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc catcagtgaa
    18901 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
    18961 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
    19021 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
    19081 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
    19141 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatc ttttggagct
    19201 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
    19261 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
    19321 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
    19381 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
    19441 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
    19501 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcttcttc tttatttctc
    19561 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaattgaa
    19621 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
    19681 tactgaactc tgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
    19741 ttgtcaaaaa gaaatcacac cggctcaatc cgcttccgcc taacatcgtg taagctaact
    19801 tcgcgtactc ttgtgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
    19861 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
    19921 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
    19981 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
    20041 tttgcagctc ttctcggttt tctgcctttg taatgccgac actagaaccc atattcgcag
    20101 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
    20161 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
    20221 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
    20281 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
    20341 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
    20401 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
    20461 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
    20521 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
    20581 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
    20641 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
    20701 aaaataaacg aataacttat ttataaaaaa acgagttggt ttctaaaaga acaaacgtca
    20761 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
    20821 aggagggatg atgatggcgc aaacaaatcc acgtagccgc ttggcagaaa tcattcgagt
    20881 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
    20941 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
    21001 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
    21061 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
    21121 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
    21181 tcgtgccaag ctgaagaacg gtgactcggt cgtcgttaag atccaacacc ctaacatcgc
    21241 cgaagagatt cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
    21301 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
    21361 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
    21421 tggctggaaa gagatccgca gccccaaaat ttatgacgct ttttgttcga aaaaagtgat
    21481 cgtgatggaa gaaatgtctg gtaaaaactt gaatcatttg atgaatgccg aaaacggaac
    21541 agaaactttt atcaccggta ttcaaaacaa acagctgaaa caagaagttg caaagctttt
    21601 agttgagaac tttatgaaac aggtatttga tgatggtttt ttccatgctg atcctcatcc
    21661 agggaatctg ctattccatg tactaacaaa agaagaacag acacgagcta gccaaaaaac
    21721 tgaaacagtc catgaaaaag agttcggttc ctttactttt cgagcctcta cttctacaga
    21781 agaccctgtt gcgccatata ccgtcaacta catcgatttt ggtatgatgg ggcatctttc
    21841 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacaa aagatgctta
    21901 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
    21961 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
    22021 caacttgcaa gaagtattca cacacgtcgt gacgatttgt catcaaaata atctgcagtt
    22081 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
    22141 ccttgaccct gaagtttccc tcatggaagt cgcttcgcct tttgcccaac attattttct
    22201 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
    22261 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggactttggg
    22321 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
    22381 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
    22441 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    22501 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    22561 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    22621 agggattttg cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    22681 ggctacttct gaagcaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    22741 agaacaatca aaaaggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg
    22801 tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg gtaaaccttg
    22861 gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt
    22921 tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag
    22981 aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc
    23041 cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt
    23101 aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg
    23161 gacatgatta gcgtattctt tggtcgtata atggtccaca ctggttacga taatcacgtc
    23221 gagaccatag gattctccta attttatgac atcttctttt actggagaac catctccatc
    23281 aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc
    23341 tcagtaagag accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg
    23401 acactgattc atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg
    23461 ctaaaataaa caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag
    23521 tagtatactt gacaacgatc cggcgatcca ttggaatagc tgcatcagtt tgataatatt
    23581 ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg
    23641 caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag
    23701 ccaatggaga ccatgataaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca
    23761 agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc
    23821 caacataaaa actgctaaac aaaaaaagga aattccctag cattccattt agaaaagtaa
    23881 acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga
    23941 acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata
    24001 acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct
    24061 ctcgcgtagc taccagattt cttttgatct ctttgaaatc cagttcgtaa cgcggataat
    24121 gactaaccgt atggtaggcc ataacgtaaa aaagtgtata aatagtaaag cttgctgcaa
    24181 tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa
    24241 ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg
    24301 tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa
    24361 ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca
    24421 gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata
    24481 gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg
    24541 agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa
    24601 agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg
    24661 ctcccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    24721 accaatttta cagaatatat tcttgtaaaa aaaaatgcag aaagaagcat aagcttcttt
    24781 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    24841 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    24901 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    24961 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    25021 tgaccgatat ctgaaacaag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    25081 attgcttgat gatttccctg gttcactcgc aaaaagatct ctctttcctt tgtttgcgca
    25141 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    25201 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    25261 accgtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    25321 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    25381 ggcacaaaag cttccggcca tacttcttgt atgactgcca tcagctcatc caattcattt
    25441 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    25501 gcttgatgga ataaaaagag catgatcatg ctatacacac ctaccgacca tggtttcaag
    25561 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    25621 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacga ctagtacttt tttgatatct
    25681 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    25741 tggacaaagg ctacgccata gtaacggaca agtaaaaatc cgattgcttc cccaatcact
    25801 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    25861 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcag cagaacaaat
    25921 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    25981 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaacaacag accaataatg
    26041 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    26101 atcttttttc atttattaaa actgcctcct
//
LOCUS       DAJSVF010000032        25828 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSVF010000032 DAJSVF010000000
VERSION     DAJSVF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.033"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(29..574)
                     /locus_tag="ON550_001373"
     CDS             complement(29..574)
                     /locus_tag="ON550_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3954467.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ON550_001374"
     CDS             complement(586..1278)
                     /locus_tag="ON550_001374"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR3954468.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /locus_tag="ON550_001375"
     CDS             complement(1297..1581)
                     /locus_tag="ON550_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954469.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ON550_001376"
     CDS             complement(1594..2157)
                     /locus_tag="ON550_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3954470.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ON550_001377"
     CDS             2385..3047
                     /locus_tag="ON550_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR3954471.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ON550_001378"
     CDS             3171..4367
                     /locus_tag="ON550_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR3954472.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ON550_001379"
     CDS             complement(4468..5844)
                     /locus_tag="ON550_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3954473.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="ON550_001380"
     CDS             complement(5881..6747)
                     /locus_tag="ON550_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR3954474.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="ON550_001381"
     CDS             complement(6836..7861)
                     /locus_tag="ON550_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3954475.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ON550_001382"
     CDS             complement(7879..8553)
                     /locus_tag="ON550_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954476.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ON550_001383"
     CDS             complement(8737..9630)
                     /locus_tag="ON550_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3954477.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ON550_001384"
     CDS             complement(9704..11401)
                     /locus_tag="ON550_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3954478.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11394..12707)
                     /locus_tag="ON550_001385"
     CDS             complement(11394..12707)
                     /locus_tag="ON550_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3954479.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12707..16744)
                     /locus_tag="ON550_001386"
     CDS             complement(12707..16744)
                     /locus_tag="ON550_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3954480.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16757..17215)
                     /locus_tag="ON550_001387"
     CDS             complement(16757..17215)
                     /locus_tag="ON550_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3954481.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17513..18031)
                     /locus_tag="ON550_001388"
     CDS             complement(17513..18031)
                     /locus_tag="ON550_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3954482.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18033..18650)
                     /locus_tag="ON550_001389"
     CDS             complement(18033..18650)
                     /locus_tag="ON550_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3954483.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18647..19501)
                     /locus_tag="ON550_001390"
     CDS             complement(18647..19501)
                     /locus_tag="ON550_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3954484.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19498..20409)
                     /locus_tag="ON550_001391"
     CDS             complement(19498..20409)
                     /locus_tag="ON550_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3954485.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20450..21784)
                     /locus_tag="ON550_001392"
     CDS             complement(20450..21784)
                     /locus_tag="ON550_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3954486.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21992..22822
                     /locus_tag="ON550_001393"
     CDS             21992..22822
                     /locus_tag="ON550_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCR3954487.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23066..23384)
                     /locus_tag="ON550_001394"
                     /pseudo
     CDS             complement(23066..23384)
                     /locus_tag="ON550_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23506..23700)
                     /locus_tag="ON550_001395"
     CDS             complement(23506..23700)
                     /locus_tag="ON550_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954488.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24205..24390)
                     /locus_tag="ON550_001396"
     CDS             complement(24205..24390)
                     /locus_tag="ON550_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954489.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25240..25581)
                     /locus_tag="ON550_001397"
     CDS             complement(25240..25581)
                     /locus_tag="ON550_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3954490.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25581..25811)
                     /locus_tag="ON550_001398"
     CDS             complement(25581..25811)
                     /locus_tag="ON550_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3954491.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         7640 a         5538 c         4422 g         8228 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct
    11461 agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat
    11521 tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc
    11581 tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct
    11641 aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc
    11701 caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc
    11761 acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta
    11821 acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg
    11881 tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc taaataatat
    11941 ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt
    12001 tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca
    12061 tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt
    12121 ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct
    12181 ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt
    12241 gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc
    12301 gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc
    12361 gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca
    12421 tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt
    12481 gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg
    12541 tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc
    12601 acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc
    12661 ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct
    12721 cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg
    12781 cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag
    12841 gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta
    12901 actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt
    12961 ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc
    13021 gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca
    13081 cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt
    13141 ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct
    13201 caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt
    13261 ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt
    13321 catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact
    13381 taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg
    13441 cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta
    13501 aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac
    13561 gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag
    13621 aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta
    13681 attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata
    13741 aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct
    13801 tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat
    13861 caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg
    13921 tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt
    13981 tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc
    14041 ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt
    14101 ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac
    14161 tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca
    14221 gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa
    14281 gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc
    14341 tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg
    14401 ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc
    14461 cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg
    14521 gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt
    14581 cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct
    14641 cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg
    14701 aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct
    14761 gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt
    14821 ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat
    14881 aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt
    14941 gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg
    15001 gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa
    15061 aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat
    15121 cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc
    15181 catcttcttc taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt
    15241 gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct
    15301 cttgatactt atttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt
    15361 cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag
    15421 tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt acttcatctt
    15481 ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat
    15541 tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt
    15601 ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg
    15661 gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc
    15721 tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa
    15781 gcggagttcc tccgattggt gtacgattta ttgctgtatt ctcacttccc gtcaaacgat
    15841 tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg
    15901 tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca
    15961 gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg
    16021 tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg
    16081 gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg
    16141 catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt
    16201 tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa
    16261 gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag
    16321 tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca
    16381 agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc
    16441 aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt
    16501 tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa
    16561 gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg
    16621 gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg
    16681 aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca
    16741 ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt tcaaaaatca
    16801 atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga
    16861 tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa
    16921 cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt
    16981 cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa
    17041 tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt
    17101 ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc
    17161 caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct
    17221 tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgcccatct
    17281 aattataatt taatttaatt ttaattttaa atcaaatgat atccttttta gttaaaaaac
    17341 accaattata caacatttat tattaaaatt aaattataaa attttataac gttctcattt
    17401 aacaaaactt aagttattaa gacaaatgaa taaacaacta cgctttcctc gaaacaaaaa
    17461 aataaagcac aaatgcgcat ttacgcactc atgctttatt cgtacaatta attcagataa
    17521 cggcagcgca taggatgcca ataacacttc ctaaaatagc tcctcctaaa atgtcagtag
    17581 gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat aaaaaggcta
    17641 atattcctgc aactggactt aattcgaaca acgcagctac tgataaaaag gctagcgctg
    17701 tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca agccgacgat
    17761 ttttttcata aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa ccaacaaaaa
    17821 aatagatcaa actgccgata agaatggcta gaccatattc tctagtatca ggtatcaaga
    17881 ctaaaacagc agcaaggagc aaccaaataa aaccaccatt agagatatta ctgataaatt
    17941 tcaaaaaatt gatttttcga ctatccgttt tccgttgctt ggcgaacttg ttgataatac
    18001 tatcatcgat tttagcaatc aaacgcaaca tgttattcct cctttttaag aaccttttct
    18061 tgcgtacgat ggataaaaag caggacttga aaataaaaca agaaactgaa acctaataaa
    18121 gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt gcctgccaaa
    18181 tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat ccaagaagct
    18241 tctttttgtt ccagttgaag caagattttg taactaaaga gaagcgcgct taatagaagc
    18301 aaaaacaacg tagtatacat caatgtatag ccaattcccc caccccaata gcggaaaatc
    18361 tgataatcga tcaatagaag aagaaaataa acacttgcta aactatcttg ctttccataa
    18421 agagagaagt tctcacgaaa ctttttccag aatagtgaac cgaacttttt ctcttcattt
    18481 tttttgtaat gataaatcat atacagaact gttcgacttg cagttcctaa tgtgaaaaca
    18541 ggcaagacgc acaggaacca tagtagctgt aaatggataa gacgtggaat ccattcaatg
    18601 atttctaaga aataatcatt ggctttttta ccaatcgatc gattcatcat ccttttgaac
    18661 ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc aaaaaagaac agcaaaatcg
    18721 tcgggatcaa gacaagtgtt gctcctgcca tcagataatt ccattgtgtc gttgcttggt
    18781 tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg atatataaca
    18841 gaggacctaa gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg gtaatcaagg
    18901 caggtttcgt caatggaagc atcaaatgac tccaaatata caaatgattc gctccgtcga
    18961 tttcagcagc ttcgatcaat tcattattga tcgataagta aaattgacgc atcagaaata
    19021 tattgaacgc attgccgaag aacgatggaa cgatcagggg caaataagta cctacccagc
    19081 cgatcttgga gaataataca tattgaggga tcatcgttac aaaccccggg atcatcatcg
    19141 tagaaagaac caaagcaaac atcagctttt tcccaggaaa atcaagcttc gcaaatccat
    19201 aagcaataaa agaatttgaa aggacattac cgatcactac tagcacagtg atgaataatg
    19261 tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt atccagatag ttttcccata
    19321 cgattttttc tgggaaaaag gtcggaggat aacgaacgat ttcttccatt ggtttcaatg
    19381 aagtaaacac catccaaagc aatggaatga tcaaaatgat tcctattact gtcatgagga
    19441 cataacttag tatttttttt gtttgccgat tcacactggt ctttttgaaa taatccatca
    19501 tttgccgtca cctccagaat aatagaccca cttaggtgca agcttcaaat tgacaaacgt
    19561 cagaaccaac acgatcagaa acaggatcat tgacatagcc atcgcataac ccatatcaaa
    19621 agctacgaac gccttgttcc acatatgcag attataaaaa agcaaagaat ttgcaggtcc
    19681 gcctgttcca tttttcgtca tgacataagc ttcttgaaaa atttggaaag aaccgatcgt
    19741 agaggtgatc acatcgtaaa aaataatagg agtgattaat ggcaaagtaa tacttttgaa
    19801 ctttccccaa acgcctgctc catcgatttc tgctgattca tataaagatc taggaacatt
    19861 ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa ctccatattt tcatcaaaat
    19921 caacgcaggt ttcgtccatt gtggatcaaa cagccaagct ggtccaggga tatggaacca
    19981 gcccagaaat gtgttgatca atcctgcaga tggagaaagc agctgcatcc ataagatgta
    20041 gactgctact cctgataaga ctgccggtaa atagaaagct gtacggaata cactgattcc
    20101 tttgattttt tgattgagta ctgtggctaa aaacacactg aaaatcgtcg tcagcggtac
    20161 attgaagatg acgaaataaa cggtgttgta taacgatttc caaaataaat catcgtgaac
    20221 gaacattctt ttgaaatttg ctagaccaat gaaattcatc ttggatgtca aattataatc
    20281 ggtgaaacta ccaataactg aaaaaagcat aggccctagc ataaatacca agaacccgat
    20341 aataaaaggc gagataaata aataggccca tcttgcttct tttttctttc ggatagataa
    20401 acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc aaaaatggat tattgatttt
    20461 gcttgactaa gttttctact gctttttgtg catctgccaa tccttctttt ggtgattttg
    20521 ttcctaaaag aatctggtca atctgttcat tcactaatgt cgtgtaatcc ggagcatcta
    20581 aaggaactgg tgtgatcacc gtatttttaa agttttcgtc cgccatttga tagatcattt
    20641 ttcctttatc cgttaggttg tcattattca ctaaattctc attggctgtc ttgctcgcca
    20701 tgatatcaaa acttttttcg ccgaattttt cttgtacttc tggagaagaa agatatttga
    20761 tgaattcata agaggcctca ggatcttttg ctccgtatgg tacttccaag acgaatccgc
    20821 caccccacga ccaatggcct gaaccttctt ctttttctgg gagctgcatc acgccaaaat
    20881 caaaatcatc cggtgcattt tccatcaaac tagagtaata attgatattt tgggcacgca
    20941 tggctactaa tccggaaata aatggatcag atacaccaga cccaaattcg gcttctaatt
    21001 tattgatcgt atttttccca taataatctt gccagtcttg gatccactcc aaggcttcca
    21061 ctttattttt agtatcaatc ttgacttttt catctttgtc aaaccaacta gtcccgttat
    21121 ctgcgtttag cgcccataca tcagccccta aattccataa tggataaaaa ccgatgcgtg
    21181 taaattcttt tccttctttt ttatctagtt tacgtgctgt ttcttctaat tcatcccaag
    21241 ttgtcggtag atcttgttca ctgattccag cttctttaaa taacttttta ttataaaaaa
    21301 tgacttgtgt atctgtattg aatgggatcg cataaggatc accttcatat tctactgtat
    21361 cccaaagctg cggataaaaa tccttagaag tgtctttttc tacatatttg gagagattag
    21421 ttgcttgctg tgcttctgct ctttgggcaa cggaatttat gtcctgtaca ataacatcgg
    21481 gtgcatttcc agctgttatt gcagataaag atttagtcca gatgtcaccc catggctgat
    21541 acttataatt tacatggatc tcgttttgtg attgattgaa atcctcaatg atttcttcga
    21601 tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa atcaatattt tttcttccat
    21661 cacttgtttt ttcagaagaa tttgctgcac ttgaactggc atcgcttgca ttacttgatc
    21721 cgcatcctcc taataaaaac aatgctgaac ttgccagcaa ccctgtaaac aataattttt
    21781 tcatatttta cctccaaatt ttaatgtaac cgatgccggt tttaatataa cgatatcatt
    21841 ttgttctgaa aataacaatt tataaggtat gattctaaaa aaattattaa tgataccaat
    21901 actttatata attcgcgagc gctttctttg aattttttaa tctttcgtag cagaatgaga
    21961 atgtattaaa aatagattag gaggaatttt gatgtttaga caccagaaag aattgcagtt
    22021 tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg agctgatcgg
    22081 cggccaatat ggcgagatga gtgtagctat gcagtacctg ttccaaggct ggagctgccg
    22141 aggagaagaa aaatataaag atatgctgat ggacattgca acagaagaaa tcggccacgt
    22201 ggaaatgttg gcgacaatgg ttgctcgttt attagagggt gcgcctgttg aagatcaaga
    22261 agcagcgatg caaaaagatc caacaatcgg tgccattatc tctggaatga atccacagca
    22321 cgcgatcgta gctggcttag gacctcgtcc tgctgacagc gtgggcaatc catggagcgg
    22381 tgcctatatc attgcgagtg gcaacttgtt agcagacttt agagctaatt taaatgccga
    22441 atcacaagga agacttcaag tagcacgtat ttacgaaatg atcgatgatc gtggcgtaaa
    22501 agatatgctg tctgttctcc ttgcaagaga tagctatcat caaaatctat gggaatgtgc
    22561 agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc ccaagtactt tcccgcgtga
    22621 caaagaacga aaagacattg cttatgactt ctataacttc tcagagggag accaaagtag
    22681 tgaaggttct tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc aatggttgaa
    22741 agaacctaag atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa aaatgtatgg
    22801 gacacctcca ttcaagaaat aaatatctac ccgtagaaac acccgttttt tgttccacac
    22861 gctttgtgag caaaactgac taaagtcatt aatcataacc acccttcaaa agggtggttt
    22921 tctctagagc tataagctca aatcaccggc cagcgcctaa agacgctggc tttcatattt
    22981 tatgttcaag ccatattgcc tttgccagtt ttgcccacct cttaaaaggt ttatgtacta
    23041 ctccttagcc cttaaaagga tcgtcttact ctcgtacact cagcttatct aacgcactat
    23101 catgtttttc ttgttcttga atgtactttt tgatggtcgc ttcattttgt cctacttttg
    23161 tcacatagta tccttccacc caaaagtgtc gatacccaaa tttatatttt agatttgcac
    23221 aatctcttga tgatctcctg aatattggct cgatattgat tataaattat ttttcttcta
    23281 tactttggtg tgaatacaat gtggtattta cacatctact ttgtacgggc taaactattt
    23341 ccctttattg ctccacccgc atagcaagtg gttttgtatt tcaatcgctc ggcgcgctca
    23401 attaggtcta aagacaataa atctaaaaac ccgctgcttc actgacagaa acaacgggtt
    23461 ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat ctattttatt tttttgattt
    23521 tttataaatg gaaaatgctt caagaagtcc aagaaataaa gccgtataaa aacaagaacc
    23581 tatgaaatct ttatttatta gatattcaac agaaatcccg ataacactag ccaacaatat
    23641 atttatcaga agtgtaaaat aaggatgctt tttatagaat agaactagtg ttgttttcat
    23701 agactctcct ttcttcatat ttccaattaa tcacaaattc acggcattac tagaaaaatt
    23761 aaaggggcgc agaagtggaa aaatactctt cgactttccc tacaaaccag gccatcttgt
    23821 gtttaataac tataccaaat ctaaggtacc tctaaatcta aattccattt gtggcggtga
    23881 cctccaattt ttaactcttc tatctcccat ccagtataca taaccttgat acttcgacca
    23941 cttatatatc gaacacacaa atacctatcc attgcaggag tactacctaa cttagttgct
    24001 acttgcatct aaataacagc tgaaaagata ggacttactc ttagtaaagt atctgaacta
    24061 ttaattttta aactatcatt tttatcctca actacatgca catctgttgc aacttcactt
    24121 tctgtctcat ccagataaat gattaaggaa tcgggaattg caatatcttc agctgacgct
    24181 ggaatagttg agctaaatta gtcatcatat ctttattata aaataaccaa cagcaaaaaa
    24241 tacaatgaaa aacaaacttg gaactaatgt atcattaata aatgttgaaa aattcagcaa
    24301 ctcattactg tatgatttta gcagcccaac caacatatac cctataaaag ctaataagat
    24361 ggttaggaat cctttagaat ttttactcat tcatagacac ttccttaaat aacttttatt
    24421 atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaacctta
    24481 cattatactg gaatgaactg tattgatagt gtacctaaaa gctctactga cataagatcg
    24541 tgtaatcggt aaagtaattt tattaattat acgggatgta atcaattaga gaataaacta
    24601 atcataaaag gtacttttac attcataatt gtcgctttga gattctactt tggttcacta
    24661 tctgacccaa ttgcacattt tcgatcatta caagtattgg tgtcgttaga acaactaaat
    24721 aaaaactagc ggtagatttt tcagaatcta tgctaatttc tgtatggatg ttccagtgat
    24781 ggcaaatttc tcttttcatt gtttttcttg tcgcatgata tgcctttttg tgttattcag
    24841 ttaaactata gaaattcttt cctagacaga tcttgatccc aaattttacg tttttgagca
    24901 tcgatatagt ttaaaatgac attactcata tctaaagagt ttagattttt taggactaaa
    24961 actgtcagtt gttctttttc ttctttctgt taaagtattg tcgcgacttt tcgtatacgt
    25021 taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa aacgaatgac ttgaggaaat
    25081 cacctcaatc attcgtttaa aatgctatat attctttagt ttaagatacg gaaattaatg
    25141 ccatcgacca cactagtaga atgaacgaca tttacttggc ctttttttaa tttcgttatg
    25201 tttagcatat aaaaatatta tagagtttac ttacaatgct taaaacatat actgtataat
    25261 ctgatcaatt ttagccatgt cttgcaatgg taagttttct atttttttga gttttcgttc
    25321 cttaaaatca agagatttta attgtgtaat aattacttgt ccttttatat ctagctcttc
    25381 aggtaatgag taacgtgtcg gtaaattctt tatagtggaa gtaattggac atatgaccga
    25441 aaacattgtt ttacggttaa attcataacg agagacgact agtcctggtc gtcttttttg
    25501 aatttctttt cctacggagg gatcaaaatc aatccataca atatcacctt ttttaggaat
    25561 ataaattctt tcttctgaca ctataaaatt tctctccctt caggagttaa atcttcccat
    25621 tcgtctttct cataatactc ggcctcttcc ccaccactaa atggatcttc aatttttgga
    25681 actaacgtaa ttgttccatc atcagaatac acaacgatat attcttggtt ttctgaaggt
    25741 ttctttccgt tatcagaagg taaagttacc actacagaac ttccttgtaa tctagatttt
    25801 gtaacaagca ttatcatcac tcccttaa
//
LOCUS       DAJSVF010000033        25685 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSVF010000033 DAJSVF010000000
VERSION     DAJSVF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.034"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            171..515
                     /locus_tag="ON550_001399"
     CDS             171..515
                     /locus_tag="ON550_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR3954492.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ON550_001400"
                     /pseudo
     CDS             516..935
                     /locus_tag="ON550_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ON550_001401"
     CDS             complement(1027..1377)
                     /locus_tag="ON550_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954493.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ON550_001402"
     CDS             complement(1343..2674)
                     /locus_tag="ON550_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR3954494.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="ON550_001403"
     CDS             2930..3496
                     /locus_tag="ON550_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3954495.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="ON550_001404"
     tRNA            3641..3728
                     /locus_tag="ON550_001404"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /gene="bsh"
                     /locus_tag="ON550_001405"
     CDS             4062..5036
                     /gene="bsh"
                     /locus_tag="ON550_001405"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3954496.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7265
                     /gene="asnB"
                     /locus_tag="ON550_001406"
     CDS             5361..7265
                     /gene="asnB"
                     /locus_tag="ON550_001406"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR3954497.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEASEVLTPKYQTAPS
                     VDEIMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIE
                     VMKLAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYE
                     QVKALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIP
                     QAEAIDYVTV"
     gene            complement(7460..7906)
                     /locus_tag="ON550_001407"
     CDS             complement(7460..7906)
                     /locus_tag="ON550_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3954498.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8134..8283
                     /gene="rpmG"
                     /locus_tag="ON550_001408"
     CDS             8134..8283
                     /gene="rpmG"
                     /locus_tag="ON550_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3954499.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8371..8889
                     /locus_tag="ON550_001409"
     CDS             8371..8889
                     /locus_tag="ON550_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3954500.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8966..9922)
                     /locus_tag="ON550_001410"
     CDS             complement(8966..9922)
                     /locus_tag="ON550_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3954501.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10088..10894
                     /locus_tag="ON550_001411"
     CDS             10088..10894
                     /locus_tag="ON550_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954502.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10891..11880
                     /locus_tag="ON550_001412"
     CDS             10891..11880
                     /locus_tag="ON550_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3954503.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11877..12881
                     /locus_tag="ON550_001413"
     CDS             11877..12881
                     /locus_tag="ON550_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3954504.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13013..13555
                     /locus_tag="ON550_001414"
     CDS             13013..13555
                     /locus_tag="ON550_001414"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR3954505.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13575..14273
                     /locus_tag="ON550_001415"
     CDS             13575..14273
                     /locus_tag="ON550_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3954506.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14294..14506
                     /locus_tag="ON550_001416"
     CDS             14294..14506
                     /locus_tag="ON550_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR3954507.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14506..15468
                     /locus_tag="ON550_001417"
     CDS             14506..15468
                     /locus_tag="ON550_001417"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR3954508.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15486..15881
                     /locus_tag="ON550_001418"
     CDS             15486..15881
                     /locus_tag="ON550_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3954509.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16044..16409
                     /locus_tag="ON550_001419"
     CDS             16044..16409
                     /locus_tag="ON550_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR3954510.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16406..18217
                     /locus_tag="ON550_001420"
     CDS             16406..18217
                     /locus_tag="ON550_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR3954511.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18279..18446)
                     /locus_tag="ON550_001421"
     CDS             complement(18279..18446)
                     /locus_tag="ON550_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR3954512.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18694..19683
                     /gene="galE"
                     /locus_tag="ON550_001422"
     CDS             18694..19683
                     /gene="galE"
                     /locus_tag="ON550_001422"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3954513.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19695..21179
                     /gene="galT"
                     /locus_tag="ON550_001423"
     CDS             19695..21179
                     /gene="galT"
                     /locus_tag="ON550_001423"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR3954514.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21203..22183
                     /locus_tag="ON550_001424"
     CDS             21203..22183
                     /locus_tag="ON550_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3954515.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22374..23096
                     /gene="tsaB"
                     /locus_tag="ON550_001425"
     CDS             22374..23096
                     /gene="tsaB"
                     /locus_tag="ON550_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR3954516.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23080..23622
                     /gene="rimI"
                     /locus_tag="ON550_001426"
     CDS             23080..23622
                     /gene="rimI"
                     /locus_tag="ON550_001426"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3954517.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23629..24093
                     /gene="rimI"
                     /locus_tag="ON550_001427"
     CDS             23629..24093
                     /gene="rimI"
                     /locus_tag="ON550_001427"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3954518.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24090..25106
                     /gene="tsaD"
                     /locus_tag="ON550_001428"
     CDS             24090..25106
                     /gene="tsaD"
                     /locus_tag="ON550_001428"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR3954519.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8333 a         4401 c         5380 g         7571 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagc atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa
     6721 tcatgactgt tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat
     6781 atgtcgatct tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta
     6841 tggcaagttc tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc
     6901 aacaaatacc aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag
     6961 ctgcgaacaa acatcttccg gaaaaatggt cgaatcgtgt gaaattagga ttccctgtgc
     7021 caatcaaagc ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttgttcgaag
     7081 cagattttgc aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc
     7141 acgaaggacg taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt
     7201 ataaagtcta ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg
     7261 tttgatgaaa atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg
     7321 ataagaaaca atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc
     7381 ggagttggca gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc
     7441 acaacccctt tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat
     7501 aaatgaacaa agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt
     7561 tttttgtatc aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt
     7621 cttcctcata ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat
     7681 cgtgttcatc cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac
     7741 tagcatcttc ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag
     7801 ggtacccttc atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat
     7861 ctaagttttt cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct
     7921 ttattaaaaa acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga
     7981 ttttgtttga gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg
     8041 tgaaaaacac tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct
     8101 tagaggattc ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt
     8161 acttcttgta aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt
     8221 ttggaaaaac aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa
     8281 taatgttttt aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca
     8341 aaacatctaa aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa
     8401 aatggtctga tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc
     8461 tttcgcgaac atacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg
     8521 ggggatcttt cacttcctta tcgccatatc atcgtgttga caacgcagcc aaattttcgc
     8581 acgaaagaaa atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg
     8641 atctcagaag atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac
     8701 actggtcgga tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg
     8761 aatattgatt tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa
     8821 aatctatatg cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa
     8881 gaagaataaa atctatgcgt ctagtttgtt aataagaaaa aaagcatatc acgactaatc
     8941 gtgaagatgc tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt
     9001 cctgactggc aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat
     9061 gattgtctgt cactgcttta agattcttcc agcttggttc attgaacatg gcagcatctt
     9121 tgtcactgtt cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa
     9181 cagcagacca gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa
     9241 attccagatc gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg
     9301 agttgttggt cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt
     9361 ctcgagtttt cttgatggat ttttgataat cggaaatgat cttttctgcc tctttttctt
     9421 ttgccaaagc tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt
     9481 ttacaacata agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag
     9541 cggaagaaga aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt
     9601 cataagaaat cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac
     9661 tgccaacagt ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt
     9721 aactgccgat gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg
     9781 tgactgtatg agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat
     9841 ctgaagaggg ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga
     9901 atacttttgt ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg
     9961 gttattattc ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag
    10021 tagcgcttga cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat
    10081 gggtgaaatg acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat
    10141 cattgaaaac atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa
    10201 tggaagtgga aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg
    10261 aaagatcatt gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa
    10321 aattgcatta ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt
    10381 atcttacgga cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga
    10441 agcaatcgag tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc
    10501 aacgctgtct ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac
    10561 agatatcttg atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat
    10621 cctacagtta ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca
    10681 tgacattaat catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt
    10741 gatcacagaa ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga
    10801 gattgatgca acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga
    10861 tttgttgcca aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg
    10921 cttttggtct gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg
    10981 actgtgggaa aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt
    11041 caccaaatca tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt
    11101 tgttttgcat tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca
    11161 ggcttactag gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg
    11221 cctaaagctt ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact
    11281 ttgctcgtat ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt
    11341 ttagctggcg tagctattag ttcttttttt actgctatca gccaagcact tacccaattc
    11401 tttaatctgc agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg
    11461 aaacaattag cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta
    11521 ggaaaagcaa tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat
    11581 cctaaacgaa cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt
    11641 tcgttagtcg gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg
    11701 gttggaacgg actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt
    11761 atgttggcgg attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta
    11821 gtcattgcat tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga
    11881 aaaaaatata ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta
    11941 gtttaatgat tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga
    12001 aagaatcatc ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca
    12061 gtttattagc aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa
    12121 atgaattggc agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc
    12181 tttatttagg tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc
    12241 taggtggtgt tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt
    12301 tgggtatgaa tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa
    12361 cattgctatg cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag
    12421 cgggatcaat ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta
    12481 tcttaattcc gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag
    12541 aacgcgcgca aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag
    12601 ctgttgcttt ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac
    12661 tagctcctca tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga
    12721 gcggtttgtt cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac
    12781 taagtgggga agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt
    12841 atgcactttt tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag
    12901 gccaatcatt tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg
    12961 ctatttttct ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca
    13021 attgaaagta agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg
    13081 caagaaaaag gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa
    13141 gtcagcaaaa gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt
    13201 tatccaacga gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg
    13261 tcagatggcg ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt
    13321 tcttgagcca caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc
    13381 aggtattatc tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg
    13441 gtatttaaat aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga
    13501 acaatggcaa ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga
    13561 aatgggaggt tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac
    13621 cgttatggac ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat
    13681 ttccgcgact tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca
    13741 atgaatggtg gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa
    13801 tgaactcact gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc
    13861 gttttttctt gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta
    13921 acgaagcgaa tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt
    13981 tgtttatctt agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt
    14041 ttcttttatt tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt
    14101 ggggacatgt cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga
    14161 aacaacagac aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc
    14221 tttttgtaat atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag
    14281 gaagtgctta aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat
    14341 ctttatctat gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa
    14401 tctgtatgat agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa
    14461 aagagagcac cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt
    14521 attgggattg atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag
    14581 attcagcaaa aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct
    14641 gaaatcattg aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt
    14701 atcggaatcg gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt
    14761 gcgtataact taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca
    14821 ggaatcaaat tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa
    14881 ggtgctggag aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga
    14941 ggtatcgttg ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga
    15001 catatcactg tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa
    15061 actgtatcta gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca
    15121 ggggattcaa aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc
    15181 tttgaaatgg ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc
    15241 tacttaggtt tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta
    15301 ggtggaggag tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa
    15361 gaatttactt tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat
    15421 gaagctggtg tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc
    15481 attacatggt tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc
    15541 tttgggaagt ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag
    15601 aatttcgaga aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg
    15661 atgctgggca tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag
    15721 gatctattcg aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc
    15781 gtacagcaaa taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat
    15841 atgacggatg gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca
    15901 gctttaaaaa ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga
    15961 tagttgtgcc gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa
    16021 aaggtagaaa gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa
    16081 aagaggatgg ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa
    16141 aagcgcagtt agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt
    16201 ttgaccatgt accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt
    16261 tcgaagaaga aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag
    16321 tcattacttt tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa
    16381 aatttatcga aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg
    16441 attaagtgta gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt
    16501 tcttttcgat cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt
    16561 attaatgaat tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg
    16621 aggattggta acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta
    16681 tatcgaacag acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga
    16741 aaaagatcag caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa
    16801 gttgcaagaa gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag
    16861 caaaagaaaa gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct
    16921 tgttttagca agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga
    16981 ctacgcaaag aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct
    17041 ttcgaagata ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga
    17101 ttatgtcgga ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact
    17161 ggtctatcat ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc
    17221 ttattaccct cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact
    17281 aacaaaagaa aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt
    17341 tgactatctg aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca
    17401 cttgccgatt ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg
    17461 tctcggaaag catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc
    17521 tcttgcctat cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat
    17581 aaaagaagcg gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa
    17641 agactggcgt gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata
    17701 caaagaaata ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc
    17761 aatcattcgg acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga
    17821 accgccattt gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga
    17881 aatcagcagt cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag
    17941 taatcctgtc ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga
    18001 taatacagga agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat
    18061 tgatgcttca ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt
    18121 ggattggcta actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc
    18181 agataaaata gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg
    18241 ttttttagaa agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc
    18301 attgcttttt tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact
    18361 gtttttttca cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact
    18421 aatattccag atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat
    18481 tatggaacaa aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa
    18541 tccttgaaaa tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt
    18601 tactttgttt gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca
    18661 aacacaattt tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc
    18721 ggttatatcg gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg
    18781 gtagataatt tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa
    18841 ggagacatcc gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga
    18901 gtaatccatt ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat
    18961 ttcaacaaca acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg
    19021 aataaaatcg tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc
    19081 acagaagata caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa
    19141 aaaatgatga agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc
    19201 aatgtagccg gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat
    19261 cttgtcccaa tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga
    19321 gacgattatg atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg
    19381 attgctgcac atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc
    19441 aatttaggca gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg
    19501 acaggaaaag aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta
    19561 gtcgcttcaa gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc
    19621 aaagcgatta tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa
    19681 taggggaatg agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg
    19741 aagctggcgg atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg
    19801 taggagaaga tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact
    19861 tattggatca gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag
    19921 aacaagagat ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca
    19981 atgcattctt tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta
    20041 atttaagtaa gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc
    20101 cggcagagtc ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag
    20161 atccaaaaca aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc
    20221 tttgcatgga aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc
    20281 ggattattcg aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt
    20341 attacaatga acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg
    20401 aaacatttgc tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga
    20461 atgctgactt accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac
    20521 gtcatgaatt tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc
    20581 cgcaagtcat cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag
    20641 agaaaaactt gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg
    20701 atgaaacagt atctatcaga gctttttcag cagatggtac accacaccat acgattacgc
    20761 caattgcacg aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg
    20821 tttcagaaga acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa
    20881 aggagaacat tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac
    20941 cagaactagt agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc
    21001 atcgtccgtg ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca
    21061 ccgatgtcgt acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag
    21121 tattcaagcg agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa
    21181 cgtaaaaaag gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt
    21241 ttctcctgct actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc
    21301 cacaaagcaa aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa
    21361 tgcagcaaat actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct
    21421 tgaagatctt tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa
    21481 cgttacttta ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat
    21541 cgcactagga aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt
    21601 gcttttggta gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa
    21661 gtcaagtgtt caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat
    21721 gcttgctgga acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat
    21781 ggcatttcaa caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga
    21841 tttttctgtc gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt
    21901 tcctgatgcg ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca
    21961 agaaaaaggt ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt
    22021 agcgaaatat gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga
    22081 gctagcagtg gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat
    22141 tattgtggaa acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg
    22201 ggaaaatgga gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc
    22261 agcagataat catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa
    22321 aaaagtatgc tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta
    22381 cattaggaat agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa
    22441 ttcttggaca aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa
    22501 tcgaccaatt gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt
    22561 ctgatggtcc cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg
    22621 cttatacatt agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt
    22681 gcatcggtgt ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg
    22741 ctggggcata ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt
    22801 ctgcttctga tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga
    22861 gtgattgtca caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata
    22921 cagttccagc atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg
    22981 aaccagttgc agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag
    23041 aaaaatggtt ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg
    23101 tatgccagaa gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc
    23161 gatcgtcgtt acgtactaag agaattgact acagaagata tcaaagagct gctggatgtc
    23221 gaacgagaag tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg
    23281 aagtcaccgc ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt
    23341 atcggttcaa gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca
    23401 tatcagcgta aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg
    23461 aatcgctgtg agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt
    23521 tatcggaaat tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa
    23581 gaagatgcgt tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga
    23641 aaaaaagatt ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct
    23701 tatccacatg gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac
    23761 tctgattatc tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa
    23821 gtaatagatg aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg
    23881 catgcacggc atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt
    23941 tttttagaag tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt
    24001 cgtatattag gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg
    24061 agcagaaagt taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat
    24121 caagttgtga tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca
    24181 tcgttgcttc tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta
    24241 gccgccatca cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg
    24301 tgtcggcaga agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt
    24361 tgctgattgg tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc
    24421 ctgtaaacca tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc
    24481 cattgatggc acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg
    24541 gatcatatga aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag
    24601 ttggaagagt attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc
    24661 agggaaaaga taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca
    24721 gttttagcgg attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag
    24781 aagatctaga caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga
    24841 ttactaaaac acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag
    24901 gtgtagcggc caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc
    24961 ctgaggtaga actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg
    25021 gagcagcagc acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg
    25081 atccaagctt ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa
    25141 taagaacagc ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc
    25201 gtatgcaagc ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga
    25261 ttcacaagga acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac
    25321 cgaagagttg acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa
    25381 agccctattt tttacatact tttaatcatt attgttattt tgctattttt tacagcttat
    25441 ttatcattat ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat
    25501 cgaataatct agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt
    25561 tgcaaacagt gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag
    25621 tgatctaaaa ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga
    25681 tattt
//
LOCUS       DAJSVF010000034        25581 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSVF010000034 DAJSVF010000000
VERSION     DAJSVF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.035"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            178..2238
                     /locus_tag="ON550_001429"
     CDS             178..2238
                     /locus_tag="ON550_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR3954520.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2321..2890
                     /locus_tag="ON550_001430"
     CDS             2321..2890
                     /locus_tag="ON550_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954521.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3065..3871)
                     /locus_tag="ON550_001431"
     CDS             complement(3065..3871)
                     /locus_tag="ON550_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954522.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4009..5523
                     /locus_tag="ON550_001432"
     CDS             4009..5523
                     /locus_tag="ON550_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR3954523.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5926..6909)
                     /locus_tag="ON550_001433"
     CDS             complement(5926..6909)
                     /locus_tag="ON550_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR3954524.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6914..7561)
                     /locus_tag="ON550_001434"
     CDS             complement(6914..7561)
                     /locus_tag="ON550_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3954525.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7646..8278)
                     /locus_tag="ON550_001435"
     CDS             complement(7646..8278)
                     /locus_tag="ON550_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3954526.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8294..8845)
                     /gene="coaC"
                     /locus_tag="ON550_001436"
     CDS             complement(8294..8845)
                     /gene="coaC"
                     /locus_tag="ON550_001436"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR3954527.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8842..9579)
                     /locus_tag="ON550_001437"
     CDS             complement(8842..9579)
                     /locus_tag="ON550_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR3954528.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9576..10565)
                     /locus_tag="ON550_001438"
     CDS             complement(9576..10565)
                     /locus_tag="ON550_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3954529.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10823..11365
                     /locus_tag="ON550_001439"
     CDS             10823..11365
                     /locus_tag="ON550_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3954530.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11382..12467
                     /locus_tag="ON550_001440"
     CDS             11382..12467
                     /locus_tag="ON550_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954531.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12479..13285
                     /locus_tag="ON550_001441"
     CDS             12479..13285
                     /locus_tag="ON550_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954532.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13286..14113
                     /locus_tag="ON550_001442"
     CDS             13286..14113
                     /locus_tag="ON550_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954533.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14110..15183
                     /locus_tag="ON550_001443"
     CDS             14110..15183
                     /locus_tag="ON550_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3954534.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15611..18307
                     /locus_tag="ON550_001444"
     CDS             15611..18307
                     /locus_tag="ON550_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3954535.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18384..18872
                     /locus_tag="ON550_001445"
     CDS             18384..18872
                     /locus_tag="ON550_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3954536.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18884..19378
                     /locus_tag="ON550_001446"
     CDS             18884..19378
                     /locus_tag="ON550_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3954537.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19399..20208
                     /locus_tag="ON550_001447"
     CDS             19399..20208
                     /locus_tag="ON550_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3954538.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20205..21026
                     /locus_tag="ON550_001448"
     CDS             20205..21026
                     /locus_tag="ON550_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3954539.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21077..23176
                     /locus_tag="ON550_001449"
     CDS             21077..23176
                     /locus_tag="ON550_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR3954540.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23173..24291
                     /locus_tag="ON550_001450"
     CDS             23173..24291
                     /locus_tag="ON550_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR3954541.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24330..25208
                     /locus_tag="ON550_001451"
     CDS             24330..25208
                     /locus_tag="ON550_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3954542.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25399..25551
                     /locus_tag="ON550_001452"
     CDS             25399..25551
                     /locus_tag="ON550_001452"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3954543.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8087 a         4454 c         5411 g         7629 t
ORIGIN      
        1 tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa
       61 atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat
      121 catcttatga aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg
      181 tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg
      241 tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct
      301 gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt
      361 acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca
      421 aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg
      481 ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt
      541 gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag
      601 atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt
      661 tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca
      721 gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa
      781 ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga
      841 acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct
      901 gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga
      961 acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact
     1021 tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa
     1081 tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct
     1141 gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt
     1201 caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat
     1261 ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa
     1321 caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa
     1381 agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa
     1441 tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt
     1501 catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca
     1561 acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta
     1621 ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat
     1681 catgacccgt attcactcca agaactccat gctcagtcac tattacatgt tttaccatta
     1741 aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa
     1801 caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag
     1861 ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat
     1921 ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa
     1981 tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag
     2041 gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa
     2101 gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta
     2161 ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac
     2221 gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca
     2281 tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc
     2341 ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc
     2401 ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga
     2461 gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt
     2521 tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga
     2581 aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt
     2641 tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc
     2701 cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca
     2761 gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga
     2821 aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt
     2881 agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca
     2941 ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca tgccatacat
     3001 tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca
     3061 tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat
     3121 cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa
     3181 ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg atgattgttt
     3241 tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat
     3301 gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat
     3361 ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa
     3421 caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca
     3481 tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa
     3541 atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta
     3601 actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg
     3661 gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca
     3721 gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa
     3781 cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac
     3841 tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat
     3901 tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat
     3961 tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta
     4021 gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag
     4081 aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct
     4141 tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt
     4201 ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca
     4261 gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg
     4321 aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc
     4381 tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt
     4441 gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac
     4501 ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct
     4561 attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt
     4621 tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact
     4681 gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca
     4741 ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat
     4801 attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt
     4861 ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg
     4921 gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa
     4981 agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct
     5041 gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa
     5101 ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa
     5161 atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg
     5221 gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg
     5281 aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc
     5341 aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct
     5401 ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag
     5461 atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa
     5521 tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg
     5581 tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa
     5641 taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc
     5701 agatgcaagc ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac
     5761 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa
     5821 ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg
     5881 gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt
     5941 caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc
     6001 gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc
     6061 agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa
     6121 taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac
     6181 gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa
     6241 attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc
     6301 agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc
     6361 accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc
     6421 gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg
     6481 gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt
     6541 caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat
     6601 tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta
     6661 tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc
     6721 tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat
     6781 atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt
     6841 aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat
     6901 ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc
     6961 taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa
     7021 gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta
     7081 attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat
     7141 ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca
     7201 tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag
     7261 agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca
     7321 gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt
     7381 cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt
     7441 ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag
     7501 ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca
     7561 tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa
     7621 aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta
     7681 acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac
     7741 ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt
     7801 tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca
     7861 atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt
     7921 tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg
     7981 cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa
     8041 agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg
     8101 taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa
     8161 tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta
     8221 ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa
     8281 gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg
     8341 tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg
     8401 gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt
     8461 tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg
     8521 ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt
     8581 ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga
     8641 ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt
     8701 gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg
     8761 cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag
     8821 taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa
     8881 tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata
     8941 gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt
     9001 gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa
     9061 tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt
     9121 tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt
     9181 ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt
     9241 gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac
     9301 tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa
     9361 aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt
     9421 cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc
     9481 agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat
     9541 tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact
     9601 agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc
     9661 agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat
     9721 aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt
     9781 cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa
     9841 gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact
     9901 gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat
     9961 tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct
    10021 tttctggaaa aggcaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta
    10081 ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga
    10141 cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct
    10201 tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat
    10261 tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct
    10321 tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga
    10381 gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa
    10441 ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc
    10501 agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg
    10561 ttcattcgtg agacactcct tttaaaattt tcttttatca taccataaag cctactcttt
    10621 tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac
    10681 tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc
    10741 tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga
    10801 aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca
    10861 aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc
    10921 ccagttggaa cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt
    10981 cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta
    11041 ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct
    11101 cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg
    11161 agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga
    11221 attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta
    11281 tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat
    11341 tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt
    11401 catttgatca agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg
    11461 agatcgaaca aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga
    11521 tcctacgtat tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga
    11581 aacggttgaa tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg
    11641 cactttttcc tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa
    11701 tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg
    11761 gatacgaaaa acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg
    11821 cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg
    11881 atttgaaact acgtacagat atgcagtatg aactgcgaga tctgcaacaa cgattaggaa
    11941 tcacgtttat ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt
    12001 ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc
    12061 caatcaatca tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga
    12121 tcgaagataa tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc
    12181 gtctaaatga accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag
    12241 ataaaggaaa actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa
    12301 tcatctgtta tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag
    12361 aaggaagcaa agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg
    12421 agtctgaaga agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat
    12481 gaaaacaatg cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat
    12541 cgcgcctgta ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct
    12601 gaataattat ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt
    12661 atggtatgca tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt
    12721 tttaacaaaa ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt
    12781 gaatctgctg cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa
    12841 tcatttcttt gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt
    12901 tctgttcgtg gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt
    12961 ggaagagatg aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga
    13021 gaccttccga cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc
    13081 agtatttatt ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt
    13141 gatcacttta ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg
    13201 ttctacaatc ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga
    13261 gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg
    13321 gatcgtcttt ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga
    13381 aggaggaacg atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga
    13441 agatacacgt ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt
    13501 gatcgccacg atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg
    13561 tgcacggaca gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat
    13621 cggtgctagt ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt
    13681 attgctttcc catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt
    13741 gcaggaaatg aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca
    13801 agtagtcaaa aatatcattt tgccttatct ttctccagga atcatagctg gatattttat
    13861 ggcatttacc tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt
    13921 tacgacgctc tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa
    13981 tgcgctaagt gcgttagttt tcttgttctc catgatattg gtcattggat actactttat
    14041 tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc
    14101 acggctgcga tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg
    14161 ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt
    14221 accatctata actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa
    14281 tatggataca aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc
    14341 cagcaaggtg ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg
    14401 atcaaagaaa aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt
    14461 gatccacgat ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt
    14521 tggggaactt tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat
    14581 tgggatgatt tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga
    14641 gaagttatgg ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca
    14701 cagctgacag aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg
    14761 gcagatgaga tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct
    14821 ggcgaagctg ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa
    14881 ggatccaatc tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt
    14941 gcctatgatt tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat
    15001 attggttatt ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg
    15061 gacgaacaat tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta
    15121 ggatcgaaat acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga
    15181 taatggcaaa actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc
    15241 ctttttctat ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg
    15301 tgttatttta ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta
    15361 aaatagggcc cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt
    15421 aattggaaac tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat
    15481 gaatccagtt caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta
    15541 aattggtaga taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc
    15601 aaagtaaaaa ttggaataca aatatagaat agacatattg cagccgatag tttattatgc
    15661 ttttagacac attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga
    15721 tgagctgata cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa
    15781 tctaggtatc tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa
    15841 ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt
    15901 agataatacg gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct
    15961 gagagaggtc attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt
    16021 attaataaat ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata
    16081 cgctatccat gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga
    16141 ttatgcggac aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt
    16201 tacgggggct gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt
    16261 tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga
    16321 tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt
    16381 tattttcgcc acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat
    16441 tcaaattagt gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct
    16501 gattcaatta ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag
    16561 agaagccttg tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa
    16621 tatcattaaa atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat
    16681 tgggaaaaat gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc
    16741 gattgctagt ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt
    16801 tgattatgat tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga
    16861 aaatttttct tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc
    16921 ttttttatca atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt
    16981 tttaggaaaa acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc
    17041 ctatgtcatt tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc
    17101 aaatctacct cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc
    17161 agaaaaggac gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga
    17221 tgaatccatc gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat
    17281 tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt
    17341 agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa
    17401 tacagatagt ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat
    17461 caagaacgat ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact
    17521 tgatattggc ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc
    17581 agacaagtat tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat
    17641 catttcctgt atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca
    17701 tttatcggat gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat
    17761 cgaccaaaat gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct
    17821 tccacgttcc ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc
    17881 agccaatttg gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat
    17941 tcaggagcta gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa
    18001 cccaacgatc gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca
    18061 tttcacttta aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga
    18121 acgtttattc ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga
    18181 tgaagatgaa ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa
    18241 ttttaaagtc aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca
    18301 aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa
    18361 tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa
    18421 aagtgaacag aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc
    18481 acttagcttc aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta
    18541 cggtgattaa tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa
    18601 accaggtgaa cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca
    18661 atcagaagat taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct
    18721 ttaatctgcc aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata
    18781 ttcaggaact ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac
    18841 atgtagtaaa tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg
    18901 aattgatgaa cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt
    18961 aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt
    19021 gaagatggct gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca
    19081 tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa
    19141 agatgcggta aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa
    19201 tgctgggaaa atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt
    19261 aacgacgtca gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt
    19321 tcagcctaca ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa
    19381 aaaggaagga gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga
    19441 gttgctggac atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg
    19501 ttagcaggag cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa
    19561 gccatcttta tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct
    19621 gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc
    19681 attgtctttc cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta
    19741 tgtggaccga tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc
    19801 atcaatctgc acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc
    19861 tttgccgttt tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc
    19921 atcatgaacg gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg
    19981 ttacggatgc tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg
    20041 gcgtatctcg agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt
    20101 atagcccaaa gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct
    20161 gctgtaaatg caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg
    20221 gaatttctaa agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt
    20281 atgcttcggt gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct
    20341 tcattaatca tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac
    20401 agtatttttc gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg
    20461 agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga
    20521 gtttgatggg accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg
    20581 ttaccgcggg actagcgatc aacttagcca atgccggaaa tattttggca ccaattattt
    20641 ttttagttat gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct
    20701 attcattagg ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga
    20761 caaaagctgc tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca
    20821 aattcaatac cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa
    20881 tcctcgatag tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat
    20941 tactagataa aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt
    21001 tattgtcatt acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt
    21061 agcaaggagg gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt
    21121 taatgctgag ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa
    21181 tattcctgat gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct
    21241 tgatgtttgc gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga
    21301 aaaaccaggt gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt
    21361 tgaagtaaac gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt
    21421 gattcaagac tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt
    21481 atcctttgct cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat
    21541 tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga
    21601 gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc
    21661 acaacaggaa gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt
    21721 aatgactttt caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc
    21781 tcagctattt gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc
    21841 actttatcaa agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa
    21901 ttggggcagc tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt
    21961 gccattcacc atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata
    22021 tggcgttaag tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc
    22081 gttatccagt gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt
    22141 tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca
    22201 acgtcggtca ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact
    22261 tggaaagtat cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga
    22321 aatttttgaa gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa
    22381 actaaaggaa tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt
    22441 ggagggaatc ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat
    22501 tcttgagcaa gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt
    22561 gatgcatgat ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa
    22621 taaagatcat ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta
    22681 tagcgaggaa atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt
    22741 tatcactgtt ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga
    22801 actaatttgt ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc
    22861 tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct
    22921 ttttgtcagc ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca
    22981 aattgatcct gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt
    23041 catttcgatt gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt
    23101 gaaaataagt gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca
    23161 aaggagaaaa agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga
    23221 gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag
    23281 ctaggaccaa gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg
    23341 attgcggata ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga
    23401 acagaatttg gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg
    23461 gatagccctg ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt
    23521 tgcgaggtga actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc
    23581 gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg
    23641 tcctcatttt tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta
    23701 tattttcacg caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta
    23761 attgatcgat taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta
    23821 gaacgttatt ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat
    23881 ccgaccgtaa cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag
    23941 tacgatgagt tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct
    24001 taccaatcaa atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt
    24061 catgcctttg gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac
    24121 cccatctgtt ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt
    24181 cgcttatttg agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct
    24241 tcaggaaata ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt
    24301 tttcacatat tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga
    24361 ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc
    24421 ccacaacaac tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg
    24481 aacaattggc aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa
    24541 cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag
    24601 gaacgctaaa gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt
    24661 gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg
    24721 tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc
    24781 atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact
    24841 gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt
    24901 tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt
    24961 accttgccca atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg
    25021 gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc
    25081 ttataaatgg atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag
    25141 gtgataatgc cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc
    25201 aaccataatt aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca
    25261 aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa
    25321 atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt
    25381 tgttgtgcgc tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata
    25441 ttgtgttact ttatgtctgt ggtaatagtt atagttgctt accaatgtat gttcattttt
    25501 cgaaatgttg acttcataat ccttggctta atgtttgaat ttttttggtg atagattttt
    25561 tcagccgtat aaaataaatc g
//
LOCUS       DAJSVF010000035        25501 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSVF010000035 DAJSVF010000000
VERSION     DAJSVF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.036"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            133..3660
                     /locus_tag="ON550_001453"
     CDS             133..3660
                     /locus_tag="ON550_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR3954544.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ON550_001454"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ON550_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR3954545.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ON550_001455"
     CDS             7448..7789
                     /locus_tag="ON550_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3954546.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ON550_001456"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ON550_001456"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR3954547.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ON550_001457"
     CDS             9146..11566
                     /locus_tag="ON550_001457"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR3954548.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ON550_001458"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ON550_001458"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR3954549.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ON550_001459"
     CDS             13000..13737
                     /locus_tag="ON550_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3954550.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ON550_001460"
     CDS             13751..14566
                     /locus_tag="ON550_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3954551.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ON550_001461"
     CDS             14579..15226
                     /locus_tag="ON550_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3954552.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ON550_001462"
     CDS             15242..15898
                     /locus_tag="ON550_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3954553.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ON550_001463"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ON550_001463"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR3954554.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ON550_001464"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ON550_001464"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR3954555.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ON550_001465"
     CDS             18240..18758
                     /locus_tag="ON550_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3954556.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ON550_001466"
     CDS             18865..19365
                     /locus_tag="ON550_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3954557.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ON550_001467"
     CDS             complement(19645..20565)
                     /locus_tag="ON550_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR3954558.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="ON550_001468"
     CDS             20730..21743
                     /locus_tag="ON550_001468"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR3954559.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ON550_001469"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ON550_001469"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR3954560.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ON550_001470"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ON550_001470"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3954561.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ON550_001471"
     CDS             complement(23383..24144)
                     /locus_tag="ON550_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3954562.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ON550_001472"
     CDS             24333..25427
                     /locus_tag="ON550_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3954563.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5383 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 g
//
LOCUS       DAJSVF010000036        23824 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSVF010000036 DAJSVF010000000
VERSION     DAJSVF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.037"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            155..640
                     /locus_tag="ON550_001473"
     CDS             155..640
                     /locus_tag="ON550_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR3954564.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            633..1316
                     /locus_tag="ON550_001474"
     CDS             633..1316
                     /locus_tag="ON550_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3954565.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1442..4087
                     /gene="polA"
                     /locus_tag="ON550_001475"
     CDS             1442..4087
                     /gene="polA"
                     /locus_tag="ON550_001475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR3954566.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4132..4968
                     /gene="mutM"
                     /locus_tag="ON550_001476"
     CDS             4132..4968
                     /gene="mutM"
                     /locus_tag="ON550_001476"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR3954567.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4932..5561
                     /locus_tag="ON550_001477"
     CDS             4932..5561
                     /locus_tag="ON550_001477"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR3954568.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            5613..5945
                     /locus_tag="ON550_001478"
     CDS             5613..5945
                     /locus_tag="ON550_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3954569.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6257..6748
                     /gene="nrdR"
                     /locus_tag="ON550_001479"
     CDS             6257..6748
                     /gene="nrdR"
                     /locus_tag="ON550_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR3954570.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6771..8144
                     /locus_tag="ON550_001480"
     CDS             6771..8144
                     /locus_tag="ON550_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3954571.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8144..9070
                     /gene="dnaI"
                     /locus_tag="ON550_001481"
     CDS             8144..9070
                     /gene="dnaI"
                     /locus_tag="ON550_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR3954572.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9199..9639
                     /locus_tag="ON550_001482"
     CDS             9199..9639
                     /locus_tag="ON550_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3954573.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9783..10484
                     /locus_tag="ON550_001483"
     CDS             9783..10484
                     /locus_tag="ON550_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR3954574.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10481..11602
                     /locus_tag="ON550_001484"
     CDS             10481..11602
                     /locus_tag="ON550_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR3954575.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11617..12849
                     /gene="pepT"
                     /locus_tag="ON550_001485"
     CDS             11617..12849
                     /gene="pepT"
                     /locus_tag="ON550_001485"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR3954576.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            12981..13889
                     /locus_tag="ON550_001486"
     CDS             12981..13889
                     /locus_tag="ON550_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR3954577.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            13924..14196
                     /locus_tag="ON550_001487"
     CDS             13924..14196
                     /locus_tag="ON550_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR3954578.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14207..15253
                     /locus_tag="ON550_001488"
     CDS             14207..15253
                     /locus_tag="ON550_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR3954579.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15504..18113
                     /gene="clpB"
                     /locus_tag="ON550_001489"
     CDS             15504..18113
                     /gene="clpB"
                     /locus_tag="ON550_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR3954580.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18264..18950)
                     /locus_tag="ON550_001490"
     CDS             complement(18264..18950)
                     /locus_tag="ON550_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954581.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(18928..19422)
                     /locus_tag="ON550_001491"
     CDS             complement(18928..19422)
                     /locus_tag="ON550_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3954582.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19442..20662)
                     /locus_tag="ON550_001492"
     CDS             complement(19442..20662)
                     /locus_tag="ON550_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954583.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20809..22644
                     /gene="lepA"
                     /locus_tag="ON550_001493"
     CDS             20809..22644
                     /gene="lepA"
                     /locus_tag="ON550_001493"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR3954584.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(22738..>23824)
                     /locus_tag="ON550_001494"
     CDS             complement(22738..>23824)
                     /locus_tag="ON550_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3954585.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         7906 a         4292 c         5062 g         6564 t
ORIGIN      
        1 ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct ctgtaaacta
       61 taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt ggtacaataa
      121 tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc cgagaaaact
      181 aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag aatctattga
      241 agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt tgtatatcca
      301 acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag tgatgctgat
      361 ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac atgttgtcaa
      421 tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt ttcagtttga
      481 ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag cggagaatac
      541 gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt
      601 ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag
      661 gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt gcccttagtg
      721 cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact tttatcaata
      781 attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta atggttctag
      841 gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc tactctcttt
      901 tgaatggcat aacattagcg gttactcttg catactacga tattggaaca gtaacgcaag
      961 ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa
     1021 gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg atcattgcac
     1081 tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc tccctactgt
     1141 tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt aatatctatg
     1201 tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg aagctgtatt
     1261 tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac aactaaaaaa
     1321 gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg ctttttcttt
     1381 tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag gagttaagaa
     1441 gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc gcgcattctt
     1501 tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata caaatgcaat
     1561 ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga ctcatgttct
     1621 ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg agtacaaagg
     1681 tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac gtgatttgtt
     1741 gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg atattatcgg
     1801 tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt caggtgatcg
     1861 ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa aaggtgtcag
     1921 cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt tgacgccaaa
     1981 acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc ccggtgtgac
     2041 taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag tcgaaggaat
     2101 ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga taaatgacaa
     2161 agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc ctgtggatgt
     2221 ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc cgttttataa
     2281 agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag aagtaaaaat
     2341 ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta ctacggatat
     2401 ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg tcggagtagc
     2461 gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg aaaaccgagc
     2521 gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg cgaaacgaac
     2581 ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg atgtcttgct
     2641 aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa tagctgctca
     2701 ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg cgaaaaaagg
     2761 tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag ctgcaatcaa
     2821 tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag atcttttccg
     2881 gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg ggatcaaagt
     2941 agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa gagagatcga
     3001 acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa agcaattagg
     3061 agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa ctggatattc
     3121 cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag aggatatcct
     3181 cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc tgaaagtgat
     3241 ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa caggacgatt
     3301 aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag ggagaaagat
     3361 acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg actattcaca
     3421 aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag ctgccttcct
     3481 agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg aaaaggcaga
     3541 ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta tcgtgtatgg
     3601 catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag cacagcaata
     3661 catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa gaatcgtacg
     3721 cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat atctgccaga
     3781 catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta tcaatacacc
     3841 aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg ataaacgatt
     3901 aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac ttgtcttcga
     3961 ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta tggaacaagc
     4021 tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt ggtacgaagc
     4081 aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa aatgccagaa
     4141 ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg aaaaacgatt
     4201 caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc catctttgag
     4261 gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt tttaattttt
     4321 catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata tcaatttacg
     4381 aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga ggatggaagc
     4441 caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga aaaaggagca
     4501 agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga tagcttctta
     4561 ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc attgctgcta
     4621 gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt atgggaagcg
     4681 aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga acaattacgt
     4741 ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac gattcgcagt
     4801 tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt ctatcagcaa
     4861 actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt ggcacaaagg
     4921 gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga tttgttttag
     4981 gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt cagtctcatg
     5041 gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct ggaacgcttg
     5101 ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat gggcaactag
     5161 atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa aaacttgatc
     5221 agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct ctaagttcca
     5281 atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat gaccactata
     5341 tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt ttgatgaaac
     5401 gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt ccaatcgaag
     5461 aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa atacttgtgg
     5521 gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg tcaattttgc
     5581 tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat caaaaggata
     5641 aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa aatctaatcg
     5701 atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac caagcaagaa
     5761 tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat ttgactcaag
     5821 aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt gaccacattc
     5881 ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc gaagagaagt
     5941 tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt tcatctgctt
     6001 caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct cagtaatcaa
     6061 ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt acgcatgaaa
     6121 gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt ttgtgatttg
     6181 tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata aaatgcgatg
     6241 caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac gagtaatcga
     6301 cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg agaattgttc
     6361 gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa tcaagaaaaa
     6421 cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc gttcagcaga
     6481 aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg aaaaccgcgt
     6541 acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg tgatggaaga
     6601 tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc gccagttcaa
     6661 agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa aagaaaataa
     6721 taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc ttggaaaatg
     6781 cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct ccgctgagag
     6841 aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga gaagccttga
     6901 gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt cctgaaggga
     6961 tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat gatgcaagaa
     7021 aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt gatctgggac
     7081 ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc aaagactctg
     7141 ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag cttacccaat
     7201 tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa aaatttttag
     7261 atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc gaatcgctga
     7321 ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg acattcgatt
     7381 ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat gatccaatca
     7441 tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca cttggcgaac
     7501 tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa ctgcaaaaag
     7561 ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt gcggtttctg
     7621 aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt cagctgataa
     7681 aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca gaaaaaggcg
     7741 gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa tcaggtcttt
     7801 ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat aatgctagct
     7861 taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa atcgccacag
     7921 cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa tcaaaacaaa
     7981 agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag cttcctgatt
     8041 gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca gagattgacc
     8101 gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag acgtaggcaa
     8161 agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg aaatgatcgc
     8221 tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag aagaattgac
     8281 acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag aaaagcgtaa
     8341 atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc tgacgttgaa
     8401 ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac ggcaaagaca
     8461 agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc aatcggcttc
     8521 ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag ccctagattt
     8581 tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc tagtaggaag
     8641 ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag cgattgctgg
     8701 attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac aggcaatcgg
     8761 aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt tgatgatcga
     8821 tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg gtgttatcct
     8881 tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta caatggatga
     8941 attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa aagcgaaacg
     9001 gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc gcaaccgacg
     9061 caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa agcaggtcag
     9121 cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta atgcagaaac
     9181 ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt tggtctattt
     9241 tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga ttcgattgat
     9301 gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat atttgtcgga
     9361 acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt gaaactcgtc
     9421 gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa tgacggagtg
     9481 atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa aatcggtgac
     9541 aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc accgcaagtt
     9601 cctggagatt cgattattga tgtttcattg aacaaataac caataacatt ttttcaaaaa
     9661 taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata tgggaatttt
     9721 tttattccta gtctccttat tttatttgga aaatgctata attatctaga aggagtgaag
     9781 ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt gtccctgaat
     9841 ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca ttgatattaa
     9901 gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat gaatcagcac
     9961 gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt gcaaatggtc
    10021 tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt atgggaggtt
    10081 cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc ggacaagaac
    10141 gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg caagaaaatc
    10201 agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat gaaatcattg
    10261 tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg ttcgggccat
    10321 ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag cttcaacaaa
    10381 gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt caaaaacaaa
    10441 tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca gcggacgcac
    10501 tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag gagacccagt
    10561 cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga cattagacgt
    10621 tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca
    10681 tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag aaaaaatgta
    10741 tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca tggatattat
    10801 ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg agagttattt
    10861 agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg tggatcatgc
    10921 acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg attataccgg
    10981 tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg cacaaccagc
    11041 gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg ttattttgcc
    11101 ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt atgaggaacc
    11161 tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag gacgagtagg
    11221 tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag aagcgttcca
    11281 actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc
    11341 gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa gagcagatgt
    11401 ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg ctgggttgat
    11461 tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta tcgagaaatt
    11521 tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg aaacaaatac
    11581 aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct tttgccacgt
    11641 tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac gactccatcg
    11701 actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta
    11761 gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc aagcaatgtt
    11821 gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga ttttaatgca
    11881 gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat cgtactagat
    11941 gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc
    12001 cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa atcagggatt
    12061 gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc tcacggtacc
    12121 atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa attcgatgtt
    12181 gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg tgagttgcag
    12241 tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa tgtacatcca
    12301 gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt tcataatcag
    12361 cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt ccatttgatg
    12421 gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga tcatgatcgt
    12481 gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt gaatcagcga
    12541 tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat gaaagaaatc
    12601 attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga gcttggtatc
    12661 tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc ttacatgggt
    12721 attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt cgaatttgtt
    12781 tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga attgaacaca
    12841 aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat gatggaatgt
    12901 catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct cttttttgaa
    12961 gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa agttcaggga
    13021 aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa aagcagccag
    13081 aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa ttagtcaaga
    13141 aagcagaagc catagaagct gatactttag taattatagg tggagacggc acgattcatc
    13201 atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta cttccaggtg
    13261 gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa gaagctgctg
    13321 acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat gatgaagtca
    13381 ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc agtcaagaag
    13441 aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta ttgctgaaga
    13501 agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga aaatcccaac
    13561 tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac gactcagcga
    13621 ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt ttccgattta
    13681 ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc attacttatt
    13741 tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact cgaacggatg
    13801 gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga ttaacagttt
    13861 attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt tggaagaaga
    13921 gtaatggatg tattgttaca caatcttttg tggttttacc ttttcattgt gaatttttac
    13981 ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg gcgtgttcct
    14041 gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt gatctctcga
    14101 aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc tgcaggtgta
    14161 cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt cgtctaaagg
    14221 aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg ccattattta
    14281 ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt
    14341 tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag ggcgctcaat
    14401 caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc
    14461 tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga tctcggagat
    14521 ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga cagcactcca
    14581 tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg tcatccaatt
    14641 ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg gtatcctttt
    14701 gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg ttcaaatatt
    14761 ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat gggtggatac
    14821 atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc aagacaggac
    14881 tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt atgtacttcc
    14941 ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca tttcgtttat
    15001 cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg gttcctttga
    15061 atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag tttcttcgct
    15121 tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg tgtatgtgat
    15181 tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag agaataaaga
    15241 aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag aaaaacgtta
    15301 tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa tcaattttgt
    15361 ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa accatgtgta tattttgacc
    15421 aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag tgatcttacc
    15481 tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg cttcaagaag
    15541 cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc gacattgccc
    15601 atgtatggaa aatcttttta caacctaatc attttggtcg aaatttctat acggatgccg
    15661 gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag tatccagttg
    15721 tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt cgattattga
    15781 atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta
    15841 ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat aaaaacggat
    15901 tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa agagtgacct
    15961 cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat ttagtacagc
    16021 aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca
    16081 tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa cccggtgtag
    16141 ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa
    16201 atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct ggagcaaaat
    16261 tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg
    16321 gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga aaaacagaag
    16381 gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga
    16441 tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa gcgttggaac
    16501 gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc agtattctgc
    16561 gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac aacgcattag
    16621 tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg gacaaagcaa
    16681 tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct atgccaacgg
    16741 aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct gcactgaaaa
    16801 aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac
    16861 ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt
    16921 ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa
    16981 ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct caattagaaa
    17041 aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg gttcaagagt
    17101 ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc cccgtaacga
    17161 aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt cataaacgag
    17221 tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg
    17281 gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca actggtgtcg
    17341 gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa gatcatatgg
    17401 tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta gtcggagctc
    17461 ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc
    17521 cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct
    17581 tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta gtagatttca
    17641 aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg gaaggcgtga
    17701 ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa
    17761 acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca ccattgagcc
    17821 tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa cgtctggaac
    17881 atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct gaaaatgctt
    17941 atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa gtagaaacac
    18001 cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta acgattactt
    18061 tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc taatcttcca
    18121 ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc cttttttcaa
    18181 ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct tgaagctgcc
    18241 cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt ataactttca
    18301 aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa ggcagcacct
    18361 atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt
    18421 gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc gcataagaat
    18481 tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa taagaggaac
    18541 aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat caatccatag
    18601 ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca aaaagagatg
    18661 atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa
    18721 aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg
    18781 aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg agaagtcgaa
    18841 tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc aaaagcaagt
    18901 aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat ctaaaaatct
    18961 ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca atttgatcaa
    19021 acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc
    19081 gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc cgatcactgg
    19141 gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga tcgtcagccc
    19201 atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag caagagcaat
    19261 cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt gaggaagata
    19321 gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt tatataacaa
    19381 atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat catccttttt
    19441 atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct agtgacttca
    19501 gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca cggcgataat
    19561 tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct ataggttcat
    19621 ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca ttattccaag
    19681 gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca aacggaaacc
    19741 gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg atctgctctt
    19801 tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata tctccatgta
    19861 cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt cgagcttctt
    19921 gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag tagaacagaa
    19981 cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca tcttcatttt
    20041 cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca tggttttgtt
    20101 gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat cgctcaacca
    20161 taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag gaaagtttat
    20221 cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga tacaaataag
    20281 agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga tcttgcatca
    20341 atccatggtt agcatattct ggataagttc caactccagt cacaccaccc attttataac
    20401 gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc agtttatttt
    20461 cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa cggataaaag
    20521 ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg aacacatagg
    20581 aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat tcaagatttt
    20641 ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata acagtatagc
    20701 aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa attactgctt
    20761 ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat ggatataaag
    20821 aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca tatcgaccat
    20881 gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa
    20941 atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat cacgattaaa
    21001 ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat ctttcacttg
    21061 attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt
    21121 gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac attggccaat
    21181 gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa aatcgaccta
    21241 cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct
    21301 agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat tctcgagcag
    21361 atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa agctttgatc
    21421 tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat cacggacggt
    21481 atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt tgatgttaca
    21541 gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt cggtgatgtc
    21601 ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga tacagtaacg
    21661 ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt
    21721 tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga agctttagaa
    21781 aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca agcattaggt
    21841 tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca agaacgcttg
    21901 gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta ccatgttaat
    21961 aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga acctgtaacg
    22021 attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa tgattttgtc
    22081 ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat ggattattta
    22141 gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt ctttgacttt
    22201 ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga aatgtctggt
    22261 tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa agtcgatgca
    22321 ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa
    22381 ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc tattggacaa
    22441 aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat
    22501 ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg
    22561 atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt tctgaaaatg
    22621 gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa aaaataaacc
    22681 atgtttacat ggatttgcca ccggtttgcc accggtggtt tttttcggtg gcaaaaatca
    22741 agaattcttt ctaagatttt ccatgatatc gaccgtttca tttctcatct tatctgttac
    22801 atgcgagtaa gtatccatcg taattgaaat tctgctgtgg cccaatcgtt cagagatttc
    22861 tttcattttt gcgccatttt cgagaagaag tgtagcatga gtatgtctga gagaatggaa
    22921 gttaaaagag agggagagtg cattcgatat cctcctagta ttccatttca ctacacttgg
    22981 cgtaactagc tcaccgtctt ccttcgtaca tactgcattt gaatcaatgt agagctttcc
    23041 atacttcatt cgattttcta attgttgttt cttatgtttt ttcagaattg ctagcaaggt
    23101 ttgtccaata aaaatcgttc gattggaact gcttgtcttt ggcgtaccat atacccatgc
    23161 gccgtcattc tttaccattt gtttctctac agtaattgtt ccatttgaaa aatcgacatc
    23221 atcccacgtc agaccacaaa cttcgccaac gcgcattccc gtataaaatc caatattcaa
    23281 aggaatatag aaaggatgtc cttcaggagt aatttctagc atatgatcaa agtcctcaag
    23341 agaaatgatt tttagatctt ttttagtcgt tggtcgtgct tcatattttg gtatttttac
    23401 atacagcata ggattttgct tgattaatcc ccaaggataa accgccatat tcagcgcatt
    23461 cttaaggaca gagtgagtaa tagtcattgt tttcttcgag taaccctttt taaattcagc
    23521 attgatgaaa ttttgtaaaa gagcagggga gagatccgta agttttttct ttcctaaata
    23581 accgtttata tgatttttga tggtaaatcg gtagttttca taggtattgt attttagatt
    23641 tagtttaacg tattcctcca tccaaaaatc aaggtattgt tttactcgag tatccgtacc
    23701 taaaaagtat tgtcctgttt cgtcaatatc tgataaaact tttcgtaaag cagcttcggc
    23761 ctctggtcgg gtgtctccgc caactttctc cacttttttt cttgagccat catcattgat
    23821 atct
//
LOCUS       DAJSVF010000037        23766 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSVF010000037 DAJSVF010000000
VERSION     DAJSVF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.038"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            299..1960
                     /locus_tag="ON550_001495"
     CDS             299..1960
                     /locus_tag="ON550_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3954586.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="ON550_001496"
     CDS             complement(2030..2377)
                     /locus_tag="ON550_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR3954587.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="ON550_001497"
     CDS             2461..3402
                     /locus_tag="ON550_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954588.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="ON550_001498"
     CDS             3403..4446
                     /locus_tag="ON550_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954589.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="ON550_001499"
     CDS             4452..5522
                     /locus_tag="ON550_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954590.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="ON550_001500"
     CDS             5512..6465
                     /locus_tag="ON550_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3954591.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="ON550_001501"
     CDS             6477..7487
                     /locus_tag="ON550_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3954592.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="ON550_001502"
     CDS             complement(7772..8593)
                     /locus_tag="ON550_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3954593.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="ON550_001503"
     CDS             complement(8690..8968)
                     /locus_tag="ON550_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954594.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="ON550_001504"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="ON550_001504"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR3954595.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="ON550_001505"
     CDS             complement(11724..12311)
                     /locus_tag="ON550_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954596.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="ON550_001506"
     CDS             12486..13808
                     /locus_tag="ON550_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3954597.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="ON550_001507"
     CDS             13963..14739
                     /locus_tag="ON550_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3954598.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="ON550_001508"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="ON550_001508"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR3954599.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="ON550_001509"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="ON550_001509"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR3954600.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="ON550_001510"
     CDS             16629..17258
                     /locus_tag="ON550_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954601.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="ON550_001511"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="ON550_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR3954602.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="ON550_001512"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="ON550_001512"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR3954603.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="ON550_001513"
     CDS             19445..19981
                     /locus_tag="ON550_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3954604.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="ON550_001514"
     CDS             complement(20021..21784)
                     /locus_tag="ON550_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954605.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKNIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="ON550_001515"
     CDS             complement(21787..23502)
                     /locus_tag="ON550_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954606.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSIPEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7671 a         4335 c         4813 g         6947 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgttttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caggaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtt
//
LOCUS       DAJSVF010000038        23352 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSVF010000038 DAJSVF010000000
VERSION     DAJSVF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.039"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(13..2157)
                     /locus_tag="ON550_001516"
     CDS             complement(13..2157)
                     /locus_tag="ON550_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3954607.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2514..3887)
                     /locus_tag="ON550_001517"
     CDS             complement(2514..3887)
                     /locus_tag="ON550_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3954608.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3887..4282)
                     /gene="rbsD"
                     /locus_tag="ON550_001518"
     CDS             complement(3887..4282)
                     /gene="rbsD"
                     /locus_tag="ON550_001518"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR3954609.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4310..5227)
                     /gene="rbsK"
                     /locus_tag="ON550_001519"
     CDS             complement(4310..5227)
                     /gene="rbsK"
                     /locus_tag="ON550_001519"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR3954610.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5335..6348)
                     /locus_tag="ON550_001520"
     CDS             complement(5335..6348)
                     /locus_tag="ON550_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3954611.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6429..7028)
                     /locus_tag="ON550_001521"
     CDS             complement(6429..7028)
                     /locus_tag="ON550_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3954612.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7214..7915
                     /locus_tag="ON550_001522"
     CDS             7214..7915
                     /locus_tag="ON550_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3954613.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7925..10636
                     /locus_tag="ON550_001523"
     CDS             7925..10636
                     /locus_tag="ON550_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3954614.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11087..12715
                     /gene="treC"
                     /locus_tag="ON550_001524"
     CDS             11087..12715
                     /gene="treC"
                     /locus_tag="ON550_001524"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR3954615.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12775..13956)
                     /locus_tag="ON550_001525"
     CDS             complement(12775..13956)
                     /locus_tag="ON550_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3954616.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14208..15731
                     /locus_tag="ON550_001526"
     CDS             14208..15731
                     /locus_tag="ON550_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3954617.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15800..16528)
                     /locus_tag="ON550_001527"
     CDS             complement(15800..16528)
                     /locus_tag="ON550_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954618.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16541..17821)
                     /locus_tag="ON550_001528"
     CDS             complement(16541..17821)
                     /locus_tag="ON550_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3954619.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17962..18564)
                     /locus_tag="ON550_001529"
     CDS             complement(17962..18564)
                     /locus_tag="ON550_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3954620.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18809..19015
                     /locus_tag="ON550_001530"
     CDS             18809..19015
                     /locus_tag="ON550_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954621.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19327..20046
                     /locus_tag="ON550_001531"
     CDS             19327..20046
                     /locus_tag="ON550_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3954622.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20127..20810)
                     /locus_tag="ON550_001532"
     CDS             complement(20127..20810)
                     /locus_tag="ON550_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR3954623.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21028..23214
                     /locus_tag="ON550_001533"
     CDS             21028..23214
                     /locus_tag="ON550_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3954624.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7769 a         4204 c         4578 g         6801 t
ORIGIN      
        1 ttttaattta ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa
       61 caaggttagt acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg
      121 tcttgccgta aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc
      181 gttgttgacg cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt
      241 ccatttgatt ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc
      301 agttgttgct gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat
      361 cgcgttcaat ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt
      421 tgcttctttg tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc
      481 agtcagtcca tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata
      541 tttacggaat tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt
      601 gaaggtcacg ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc
      661 gcttgataat aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga
      721 tgcgtagatt ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa
      781 ttgatcagtt gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga
      841 tgagccgcct gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc
      901 aaaatcaggc gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc
      961 atggataaat tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg
     1021 tccccagaag acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata
     1081 ttgctgttcg cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata
     1141 agtcaaacca gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc
     1201 aagatgcttg aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa
     1261 tacagcatca attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat
     1321 accgctttga tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga
     1381 accattgttt ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg
     1441 gtttgtttga ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt
     1501 tgaaccatta ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg
     1561 tgtactaggg acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc
     1621 tttaaattct gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata
     1681 atttacttct ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt
     1741 aatagtgtaa gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat
     1801 atccaaaggt tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat
     1861 caaagaagga gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc
     1921 agccggatca atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg
     1981 gtcttctgta aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt
     2041 tgtgactgtt tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac
     2101 agacggagca atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat
     2161 aaactagtcc ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt
     2221 tggaacagat aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa
     2281 ataaaacaat ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta
     2341 ttgcatagac cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg
     2401 taaattatac caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa
     2461 gaaaaagact gaaatatgtg ataaacatct catacttcag tctttttcat taactattca
     2521 taagacatta ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga
     2581 ctaacagcca gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg
     2641 gtcagactac caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca
     2701 ccagcagctg caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg
     2761 taaacagttg gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc
     2821 gaacgggttt ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca
     2881 aaattggctg ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg
     2941 atcatgacag ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca
     3001 gcgatgattg cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct
     3061 aaaccttcgc caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg
     3121 ataactaatg aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg
     3181 aggaattttt caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac
     3241 gtatactgca tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct
     3301 gaaagtaaag aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat
     3361 ccattcactt taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag
     3421 acggcattcg acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca
     3481 atcgtagtcg ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg
     3541 atcggcgtaa agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct
     3601 ttcggagctg aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga
     3661 tccatgatta tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc
     3721 tgttttaatt tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc
     3781 gcacatggaa ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga
     3841 atctgctcgg acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc
     3901 cactcactaa tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac
     3961 agttattcaa ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta
     4021 acaataattt gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt
     4081 caatcgattg tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa
     4141 gaccgtttga gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat
     4201 ccccgatact taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat
     4261 tgattacttt tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg
     4321 attgttctaa agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa
     4381 cagaagaggc tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa
     4441 gtgtgctgct taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca
     4501 ctttaaaagc agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc
     4561 ctagtgtaat gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca
     4621 aactgttatg atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta
     4681 taatgatatc tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat
     4741 tcaggatcgt tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta
     4801 agctactttc aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta
     4861 ggacttcttg ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc
     4921 ctgctgcgtc taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc
     4981 cagatgtatc gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag
     5041 ccccaacgcc tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt
     5101 tggcaccttt acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct
     5161 tgggcatgtt ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg
     5221 tgttcatttt acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa
     5281 acgttttagt aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt
     5341 caagggtgca gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc
     5401 tttttctggg aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa
     5461 gattggttgt gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc
     5521 atagccaata atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata
     5581 taatccaaaa gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag
     5641 ttctgctgtg atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg
     5701 gtcgtgaggg atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac
     5761 attttccggt ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa
     5821 gtgcatgccc gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc
     5881 ttcagatttt ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt
     5941 gattgcatct gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt
     6001 gagctcggct agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt
     6061 gacgaaattc tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg
     6121 attcgtgata tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct
     6181 agcaaaatag tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt
     6241 tttcggactg aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac
     6301 gccggaaagt ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct
     6361 atctaaatcc tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt
     6421 gccagtactt atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt
     6481 gcagttttcg gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg
     6541 caagcatctt cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca
     6601 tatacaccgt ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga
     6661 agttgaagat caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac
     6721 atattatctg tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca
     6781 gaagcagtca ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct
     6841 acgttcacta aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg
     6901 tttgtcagaa tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt
     6961 tgcatgtcga tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa
     7021 tcattcatga tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc
     7081 gtttatttgt caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa
     7141 aataaataag tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa
     7201 tggaggaatc agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg
     7261 agaaacgacc attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt
     7321 gattatactt ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat
     7381 ggatacacct gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga
     7441 taaacagctt actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct
     7501 cgttcctaat ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc
     7561 gcttgatcct gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc
     7621 gtcccaatta tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa
     7681 tcccaaactt cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca
     7741 agtattgaaa ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac
     7801 acataattct gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt
     7861 aaggtctgtt gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga
     7921 ttttatgaaa acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg
     7981 acgattcatt gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa
     8041 aactgctgga cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc
     8101 tgatttgcaa atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa
     8161 gatcccagat gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa
     8221 cgaagtgatt caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac
     8281 tgatggaaaa ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga
     8341 agggtataaa atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta
     8401 taccatcacg ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc
     8461 gaacgtcgga aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac
     8521 agatatttat tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag
     8581 caaagcttac aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga
     8641 tcgacccgaa gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc
     8701 acagcaaaaa atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga
     8761 tgctaaaaaa cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg
     8821 aacaatcgct gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca
     8881 attagttgag aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga
     8941 aaaaattgaa aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg
     9001 ttttcaagaa tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt
     9061 tttctttttc tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga
     9121 aaaccgcagg gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa
     9181 aaaatatgca atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt
     9241 aggaacgaat cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg
     9301 gagtgcgctt attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat
     9361 tgccgcattt ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc
     9421 attgcttcaa ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc
     9481 gctgtggtca cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa
     9541 gtcaagaatg cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg
     9601 cgtgggattg aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga
     9661 ctatcaagca atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga
     9721 tatgctgcag aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga
     9781 attgagaaaa aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc
     9841 tgatttggat cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa
     9901 aacaggaatc gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca
     9961 tatttcgctt tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa
    10021 aaactatcta ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca
    10081 atcgatgatg gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga
    10141 aatgctttcc gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa
    10201 acaaatcgaa acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt
    10261 cgtattatca ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc
    10321 cgagcgtgta cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac
    10381 gatgtacatt gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg
    10441 tgtaggatat atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt
    10501 tccattagtg atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac
    10561 ggttattgtg atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt
    10621 gaaatcgaat gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga
    10681 aacaaacggc aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag
    10741 ctgattgaac accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa
    10801 tactcaaata aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa
    10861 aagcttttga acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga
    10921 tagacaaata catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat
    10981 aaagataaat tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat
    11041 ataagttgtg gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga
    11101 caaagtcatt taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg
    11161 agatttaaaa ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat
    11221 ttggctaaac cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta
    11281 cacagccatc gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc
    11341 aaagaaatac cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca
    11401 tgaatggttc aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag
    11461 agaagagcca acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga
    11521 tacggggaaa tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa
    11581 ccaagcagta agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa
    11641 aggattccgt ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa
    11701 agaaaatgat ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga
    11761 attgaaccaa gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc
    11821 tacgacgatc gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt
    11881 ctttaatttc catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc
    11941 atttgatttt gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg
    12001 taacggctgg aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt
    12061 tgtagctatc gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt
    12121 gaaccgtggg acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta
    12181 ttcatcgatt tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga
    12241 acaaggattc acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc
    12301 tcgaacaccc atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg
    12361 gttaaaagtt ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga
    12421 ttctattttg tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc
    12481 acaaggagat tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca
    12541 atttgaagga cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc
    12601 catcccagaa ggattcacag aagccaatat tcttatcgat aattattccg atactgttct
    12661 tcatcaaagt ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa
    12721 aaacacaaaa cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt
    12781 aaaataatct tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt
    12841 attgtagcgt tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata
    12901 gataggaata ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac
    12961 gattaagatc gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa
    13021 gtcaccgtgg agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt
    13081 agagtcttgg aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg
    13141 aatgatacat aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag
    13201 taagaaacaa acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag
    13261 aaatgaaccc aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc
    13321 tggtgggaca atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa
    13381 agcgacaatt ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt
    13441 gctgatccgt tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt
    13501 cacgtcgtca atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa
    13561 aaattgtcgg agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt
    13621 ctttgcctgt tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa
    13681 aataatcaca taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt
    13741 gatgactggt tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt
    13801 agcgataatg attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat
    13861 cagcgcaagt aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg
    13921 gatcaaacga ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac
    13981 ttcattatag acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc
    14041 gtcaatcatt catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt
    14101 taaccatatg ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc
    14161 gaatcggaac attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga
    14221 taggtatatt ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga
    14281 aagccgagga cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt
    14341 ttacaaaagc aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg
    14401 attcccacga ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct
    14461 ttgtttataa tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag
    14521 aaggcaatgt ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag
    14581 aaggacatga tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac
    14641 atgtatggct agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga
    14701 ttgaagctta cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga
    14761 aacttcaagc actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt
    14821 ttgtcacgca acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat
    14881 cgattgctgg tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac
    14941 actttgttaa agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta
    15001 ttgccagaac gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag
    15061 gattgacgaa tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc
    15121 tcgaagcgtt gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca
    15181 aaaaagaaaa gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc
    15241 tttcagattg ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg
    15301 atcctgtttt tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag
    15361 aatattatac gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga
    15421 tggcatttac gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag
    15481 aaatcttgac ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata
    15541 aggaaagtgg cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact
    15601 ccgaacattt tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg
    15661 agaattggcc aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc
    15721 ttttgcactg ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt
    15781 ccaaactatt cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga
    15841 tggtctcgcc gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt
    15901 gtgtattgat aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt
    15961 ctttgatctt ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg
    16021 gcgctcctag cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat
    16081 cattaaactc tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa
    16141 ttttgctcag gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat
    16201 aatcagtcgg ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca
    16261 catagattat ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat
    16321 ccatttcact gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga
    16381 tcttggctgc attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta
    16441 agttttcaat catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat
    16501 cgtcttcaca aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa
    16561 tcgtttgaga aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct
    16621 ttgtcaattc atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg
    16681 agaacccttt ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac
    16741 gaatcatcac ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag
    16801 cactagctcc ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg
    16861 tatgtttcgt atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt
    16921 cctgtgccat catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat
    16981 ccaacatact tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt
    17041 gatcataata agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa
    17101 aaagcagatt ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat
    17161 ctttgatata atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct
    17221 tattagagat tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga
    17281 aaaaactcgt attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa
    17341 taatgatata gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca
    17401 cagtgtgaaa ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa
    17461 aagcggataa gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt
    17521 tcaaaacaaa aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc
    17581 caaaaggaag cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca
    17641 tcagcattcc tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa
    17701 tcaaaataca agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt
    17761 caatcaataa gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca
    17821 taaagtacag ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt
    17881 aatttttcag ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag
    17941 tcagataagt tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt
    18001 catcaagtag tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa
    18061 ataggtagaa aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat
    18121 gctgatcgcc ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt
    18181 acctcttgat ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat
    18241 tgggaaggag atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag
    18301 tagtgtccac agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg
    18361 gatgcagata taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga
    18421 aaagactaga acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt
    18481 tacttggaca tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa
    18541 tttgttcaca actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa
    18601 aaaataaaaa acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc
    18661 tcctaaaaaa atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga
    18721 aaagtacttt ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga
    18781 ccttataaaa ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga
    18841 aacatatgca tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat
    18901 ctatgaaaat agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc
    18961 tcataatctg cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca
    19021 gataaaatgt tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg
    19081 aataatgtta aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg
    19141 gcaggagaat actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta
    19201 ttttcatgaa taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat
    19261 aaagtcaagg cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca
    19321 ggaaggatgc cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca
    19381 ctaactgaaa tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat
    19441 aatgaagtat tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt
    19501 ttctttttga acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta
    19561 ctagaccgct ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt
    19621 gtttctgaag tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta
    19681 gaagaaattt cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa
    19741 actatgttag cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct
    19801 gaactctatt cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt
    19861 gatatatgtc ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca
    19921 tgtggagaac aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca
    19981 gatgcccagt cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact
    20041 aaataatata aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct
    20101 tgcttagttt ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc
    20161 cataaatgaa atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc
    20221 gaatgggcgc caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca
    20281 agaacgacac ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa
    20341 taatcatata agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag
    20401 gcacgcccgt tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa
    20461 atcgttacta atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact
    20521 gcttcataat atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca
    20581 attagatact ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta
    20641 ccaacactta gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt
    20701 tgagcagagg caaaagcacc atattggata taagtataac tgtccatggc tcctccgata
    20761 aatgtcagca aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc
    20821 accttttatg aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa
    20881 attttcagaa aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt
    20941 ataaagaaaa aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag
    21001 aagaaagatg ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa
    21061 gaattgaaac gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa
    21121 gaaatcattg ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa
    21181 gtacaagtaa aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc
    21241 tataatcgct tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt
    21301 cgaatcatag caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat
    21361 ctgatgacgc aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac
    21421 gctgcaaatg aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat
    21481 atcggaacat tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct
    21541 gaagtagcaa tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat
    21601 gctgttttgc aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa
    21661 aaaacgattg cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg
    21721 gttcagttag ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca
    21781 gaacgactgt atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta
    21841 gagttcaatg atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat
    21901 tatatcggcc aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt
    21961 caatgggaaa tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga
    22021 gatgatgatc agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt
    22081 gaaaaagaat atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct
    22141 attgtccaag gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg
    22201 atgattccag cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa
    22261 gcagaaagtc agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat
    22321 aaggaatcaa tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg
    22381 ctgaaaacga atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt
    22441 atcaagtctt tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg
    22501 caaaagaaaa atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg
    22561 aaaaaaagtg aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat
    22621 tatattttgt cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag
    22681 gaagcgttgc gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt
    22741 catctgccac gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa
    22801 gtaaagcagc atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca
    22861 gctaaagaat taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt
    22921 atccagagca ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt
    22981 aacgaaggaa gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa
    23041 ataaaagcag aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg
    23101 actagagcaa gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg
    23161 aaagtatcga gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag
    23221 agagggtgtg acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt
    23281 ctcaaatttt tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta
    23341 ttcggaaaag gg
//
LOCUS       DAJSVF010000039        22924 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSVF010000039 DAJSVF010000000
VERSION     DAJSVF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.040"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            212..1018
                     /gene="proB"
                     /locus_tag="ON550_001534"
     CDS             212..1018
                     /gene="proB"
                     /locus_tag="ON550_001534"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR3954625.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1015..2262
                     /locus_tag="ON550_001535"
     CDS             1015..2262
                     /locus_tag="ON550_001535"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR3954626.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2338..2682
                     /locus_tag="ON550_001536"
     CDS             2338..2682
                     /locus_tag="ON550_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954627.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2799..3125)
                     /locus_tag="ON550_001537"
     CDS             complement(2799..3125)
                     /locus_tag="ON550_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954628.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3498..4699)
                     /locus_tag="ON550_001538"
                     /pseudo
     CDS             complement(3498..4699)
                     /locus_tag="ON550_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4816..5331
                     /locus_tag="ON550_001539"
     CDS             4816..5331
                     /locus_tag="ON550_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3954629.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5530..5976)
                     /locus_tag="ON550_001540"
     CDS             complement(5530..5976)
                     /locus_tag="ON550_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3954630.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6023..7501)
                     /gene="msr(C)"
                     /locus_tag="ON550_001541"
     CDS             complement(6023..7501)
                     /gene="msr(C)"
                     /locus_tag="ON550_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3954631.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7855..8484)
                     /locus_tag="ON550_001542"
     CDS             complement(7855..8484)
                     /locus_tag="ON550_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3954632.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8468..8932)
                     /locus_tag="ON550_001543"
     CDS             complement(8468..8932)
                     /locus_tag="ON550_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3954633.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9419..10687)
                     /locus_tag="ON550_001544"
     CDS             complement(9419..10687)
                     /locus_tag="ON550_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3954634.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10918..11655)
                     /locus_tag="ON550_001545"
     CDS             complement(10918..11655)
                     /locus_tag="ON550_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3954635.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYSDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11722..12291)
                     /locus_tag="ON550_001546"
     CDS             complement(11722..12291)
                     /locus_tag="ON550_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3954636.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12469..13290
                     /locus_tag="ON550_001547"
     CDS             12469..13290
                     /locus_tag="ON550_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3954637.1"
                     /translation="MSRAAKSLHITQPTLSKQLKDLEEELGVTLFTRGSREIQLTDSG
                     RYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAISFISDSLHSLIKAHPDI
                     QFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLPAQDRWGLLIHRNHPLAK
                     QTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVGTYNLLYNASLMVDNGTF
                     GALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSPAARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="ON550_001548"
     CDS             13632..14504
                     /locus_tag="ON550_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3954638.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHTETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="ON550_001549"
     CDS             14501..16459
                     /locus_tag="ON550_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3954639.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSDKA"
     gene            complement(16710..17561)
                     /locus_tag="ON550_001550"
     CDS             complement(16710..17561)
                     /locus_tag="ON550_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3954640.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="ON550_001551"
     CDS             17684..18151
                     /locus_tag="ON550_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR3954641.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="ON550_001552"
     CDS             complement(18414..18896)
                     /locus_tag="ON550_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3954642.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LSESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="ON550_001553"
     CDS             complement(18996..19859)
                     /locus_tag="ON550_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3954643.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20457..21305
                     /locus_tag="ON550_001554"
     CDS             20457..21305
                     /locus_tag="ON550_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954644.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21476..21817)
                     /locus_tag="ON550_001555"
     CDS             complement(21476..21817)
                     /locus_tag="ON550_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HCR3954645.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21903..22610
                     /locus_tag="ON550_001556"
     CDS             21903..22610
                     /locus_tag="ON550_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3954646.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22597..22920
                     /locus_tag="ON550_001557"
     CDS             22597..22920
                     /locus_tag="ON550_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3954647.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
BASE COUNT         6910 a         4691 c         4150 g         7173 t
ORIGIN      
        1 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
       61 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt
      121 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
      181 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
      241 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
      301 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
      361 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
      421 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
      481 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
      541 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
      601 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
      661 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
      721 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
      781 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
      841 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
      901 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
      961 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
     1021 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
     1081 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
     1141 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
     1201 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
     1261 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
     1321 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgaccggcat catttacgaa
     1381 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
     1441 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
     1501 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
     1561 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
     1621 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
     1681 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
     1741 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
     1801 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
     1861 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
     1921 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
     1981 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
     2041 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
     2101 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
     2161 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
     2221 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
     2281 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
     2341 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
     2401 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
     2461 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
     2521 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
     2581 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
     2641 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
     2701 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
     2761 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
     2821 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
     2881 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
     2941 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
     3001 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
     3061 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
     3121 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
     3181 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
     3241 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
     3301 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
     3361 caacctcttg gtaaacggcg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
     3421 aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
     3481 tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg
     3541 atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca
     3601 atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca
     3661 aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc
     3721 gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca
     3781 aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat
     3841 gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc
     3901 gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa
     3961 tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac
     4021 aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa
     4081 aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa
     4141 ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag
     4201 accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg
     4261 attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca
     4321 aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca
     4381 gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt
     4441 tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca
     4501 tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg
     4561 tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta
     4621 caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt
     4681 gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt tattttacat
     4741 gtaaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg
     4801 agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa
     4861 ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt
     4921 cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa
     4981 gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag
     5041 gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc
     5101 tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa
     5161 cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag
     5221 aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa
     5281 ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac
     5341 agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag
     5401 ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt
     5461 tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac
     5521 agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac
     5581 gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc
     5641 agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt
     5701 caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg
     5761 atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa
     5821 aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc
     5881 ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt
     5941 tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt
     6001 cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc
     6061 cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa
     6121 acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat
     6181 caataaaatt agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag
     6241 caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt
     6301 cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt
     6361 gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat
     6421 atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt
     6481 ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag
     6541 aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt
     6601 gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt
     6661 gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt
     6721 tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat
     6781 ccggtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt
     6841 ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt
     6901 ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg
     6961 aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa
     7021 aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca
     7081 gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt
     7141 cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc
     7201 gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct
     7261 ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt
     7321 tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac
     7381 cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata
     7441 gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca
     7501 tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa
     7561 aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga
     7621 ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca
     7681 tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca
     7741 aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt
     7801 taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc
     7861 gaacgtagtg atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt
     7921 tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc
     7981 caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat cacgcgcaac
     8041 taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc
     8101 tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt
     8161 tggcatcttc cctgattcgt aagcttggtt atagatttct tcgccatatt cgtaacattg
     8221 catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg
     8281 gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg
     8341 ttgcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc
     8401 gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt
     8461 tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc
     8521 aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga
     8581 aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc tcgattttct
     8641 tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac
     8701 gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa
     8761 tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca
     8821 tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt
     8881 gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct
     8941 cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa
     9001 aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa
     9061 gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga
     9121 ttattgattc ttatcaaact atagaagata gctcttctgc agtagaaaat tttacaccgc
     9181 caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt
     9241 atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa
     9301 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
     9361 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
     9421 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
     9481 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
     9541 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
     9601 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
     9661 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
     9721 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
     9781 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
     9841 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
     9901 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
     9961 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
    10021 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
    10081 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
    10141 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
    10201 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
    10261 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
    10321 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
    10381 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
    10441 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
    10501 aaattgtatt ttctgagaca gtagctgact gcaaagtact ctctgtatcg ttttcaattc
    10561 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
    10621 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
    10681 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
    10741 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttctttttg ataaatttgc
    10801 tttttctaca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
    10861 tggtcgtttg aataactatt taacaagtaa ttctctttgg atattatccg ttatggatta
    10921 tacttcctgt gtcgtcggac ggataatcat gttactgaca gacatccggt ctggcgtttc
    10981 tatcgcaaat gctacagcag aagctatatc ttcggaggtc aatccccatt ctttttgcgc
    11041 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
    11101 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
    11161 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat cagaataaac
    11221 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
    11281 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
    11341 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
    11401 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
    11461 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
    11521 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
    11581 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
    11641 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
    11701 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
    11761 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
    11821 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
    11881 atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag
    11941 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
    12001 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
    12061 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
    12121 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
    12181 cgctgctaaa ggctcttcgt tttcagcttg gatctgatgg atcacttcaa agagcggatc
    12241 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
    12301 tccttttatc cttactttat aacggacggc ttatcatgtc taatacttat aaaacatact
    12361 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
    12421 ggatattcgt atactgacgt atttttagca gtagctagaa aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatacgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccgac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagccaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgataa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta actcataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    20221 atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc
    20281 atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga
    20341 atgaattttt gacaattcga gcaaaagtga ttatcaaata tgtaaagtca gctacttttt
    20401 tcttaaacgt ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga
    20461 ataattggct tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc
    20521 acggttttgt ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca
    20581 cggaagatta taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc
    20641 tatatgccat agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag
    20701 aacgtccaat aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg
    20761 ctagttcctc tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcacttta
    20821 tcgaacgctt caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa
    20881 ctatcatcca taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt
    20941 tcatcatgga gatgcgaaag ttcatccaag aagcagttga atcgcttatt cagcaaaatg
    21001 cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt
    21061 gggagcctga ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag
    21121 aattattagc agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc
    21181 aagtcgatca tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag
    21241 actttcggga aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa ctaaagaaac
    21301 ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc
    21361 cacaagaaat acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag
    21421 aagacttaac gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata
    21481 agtcatgaca aatttccttc gttgctggat agattttccg gcattgttga tagattttat
    21541 gatacttttc tacattttgt aagagaggtt ccactgcatc tgtatattgg ataaaattct
    21601 gacaacaact ttcaggtgtg tcgaaccacc ctaaccctag tgcagcaatc attgttgcgc
    21661 ctaatgcagg tccttcctcc acacttaaac aatagaccgt tgcatcgaaa atatcagctt
    21721 ggatttgtag ccatgcaaga tttttcgcgc ctcctcccac cgaaacaata cgtgtaaatc
    21781 tgcgacccgc tagttcttta cgtttcaatc ccaccattga caaatgctca ttaaaaattt
    21841 agaatattca caagtttaac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt
    21901 tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct accgttttcg
    21961 gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccgga tttcatccac
    22021 tagtcgtcac gttgatgtcc ctgctggtct atgctggttc tgcccaattt atcacagtca
    22081 gcatgcttgc tagtcacagc ccattgcttt ccatcgtttt ctcgaccttt ctagtcaatt
    22141 cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc
    22201 aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat
    22261 tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt
    22321 atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc
    22381 cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc
    22441 aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt
    22501 tgattctggt atatgtcgga ctgattttta tcccaagcaa cttagtgatt gtagttgtaa
    22561 cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg
    22621 ctgactattt taggttgtac tttagcaact tggctttcta gggtcctacc atttgtctta
    22681 ctgaaaaaat tcgatttacc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc
    22741 attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag
    22801 atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa
    22861 agcttattgg tgatcgtctt ggcaggaatt ctttcccttt cgttgattcg cttattataa
    22921 acgc
//
LOCUS       DAJSVF010000040        21592 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSVF010000040 DAJSVF010000000
VERSION     DAJSVF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.041"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            177..404
                     /locus_tag="ON550_001558"
     CDS             177..404
                     /locus_tag="ON550_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3954648.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            420..1586
                     /locus_tag="ON550_001559"
     CDS             420..1586
                     /locus_tag="ON550_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR3954649.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1821..2864
                     /gene="hrcA"
                     /locus_tag="ON550_001560"
     CDS             1821..2864
                     /gene="hrcA"
                     /locus_tag="ON550_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR3954650.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2958..3521
                     /gene="grpE"
                     /locus_tag="ON550_001561"
     CDS             2958..3521
                     /gene="grpE"
                     /locus_tag="ON550_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3954651.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3572..5401
                     /gene="dnaK"
                     /locus_tag="ON550_001562"
     CDS             3572..5401
                     /gene="dnaK"
                     /locus_tag="ON550_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3954652.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5552..6718
                     /gene="dnaJ"
                     /locus_tag="ON550_001563"
     CDS             5552..6718
                     /gene="dnaJ"
                     /locus_tag="ON550_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3954653.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7086..7823)
                     /locus_tag="ON550_001564"
     CDS             complement(7086..7823)
                     /locus_tag="ON550_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3954654.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7825..9327)
                     /locus_tag="ON550_001565"
     CDS             complement(7825..9327)
                     /locus_tag="ON550_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3954655.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9586..11580
                     /gene="uvrB"
                     /locus_tag="ON550_001566"
     CDS             9586..11580
                     /gene="uvrB"
                     /locus_tag="ON550_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3954656.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11590..14406
                     /gene="uvrA"
                     /locus_tag="ON550_001567"
     CDS             11590..14406
                     /gene="uvrA"
                     /locus_tag="ON550_001567"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3954657.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14669..15934
                     /locus_tag="ON550_001568"
     CDS             14669..15934
                     /locus_tag="ON550_001568"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR3954658.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15938..16669
                     /locus_tag="ON550_001569"
     CDS             15938..16669
                     /locus_tag="ON550_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR3954659.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            16804..17688
                     /gene="rapZ"
                     /locus_tag="ON550_001570"
     CDS             16804..17688
                     /gene="rapZ"
                     /locus_tag="ON550_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3954660.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17685..18683
                     /locus_tag="ON550_001571"
     CDS             17685..18683
                     /locus_tag="ON550_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR3954661.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18717..19652
                     /gene="whiA"
                     /locus_tag="ON550_001572"
     CDS             18717..19652
                     /gene="whiA"
                     /locus_tag="ON550_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR3954662.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20195..20845
                     /locus_tag="ON550_001573"
     CDS             20195..20845
                     /locus_tag="ON550_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954663.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            21032..>21592
                     /locus_tag="ON550_001574"
     CDS             21032..>21592
                     /locus_tag="ON550_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR3954664.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         7102 a         3658 c         4711 g         6121 t
ORIGIN      
        1 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
       61 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
      121 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
      181 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
      241 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
      301 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
      361 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
      421 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
      481 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
      541 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
      601 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
      661 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
      721 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
      781 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
      841 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
      901 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
      961 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     1021 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     1081 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     1141 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     1201 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     1261 atattcgtta ccgcaacaaa ggaccgatcc aacactattt acgcccatta cgagcaaatg
     1321 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     1381 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     1441 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     1501 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     1561 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     1621 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     1681 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     1741 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     1801 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     1861 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     1921 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     1981 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     2041 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     2101 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     2161 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     2221 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     2281 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     2341 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     2401 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     2461 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     2521 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     2581 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     2641 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     2701 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     2761 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     2821 aacttgctga ttattaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     2881 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     2941 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     3001 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     3061 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagatagat
     3121 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     3181 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     3241 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     3301 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     3361 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     3421 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     3481 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     3541 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     3601 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     3661 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     3721 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     3781 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     3841 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     3901 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     3961 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     4021 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     4081 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     4141 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     4201 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     4261 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     4321 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     4381 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     4441 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     4501 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     4561 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     4621 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     4681 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     4741 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     4801 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     4861 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     4921 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     4981 cacgatcaaa tcttcttcag gtctaacaga tgaagaaatc gaacgtatgg tgaaagatgc
     5041 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     5101 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     5161 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     5221 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     5281 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     5341 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     5401 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     5461 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     5521 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     5581 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     5641 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     5701 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     5761 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     5821 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     5881 aggaggcggt cgatcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     5941 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     6001 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     6061 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     6121 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     6181 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     6241 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     6301 aaacggcgga ccatttggtg atttgtatgt ggtctttcgt gtagaagaaa gtgatatctt
     6361 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     6421 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     6481 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     6541 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     6601 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     6661 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     6721 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     6781 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg
     6841 tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa
     6901 ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg
     6961 gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta
     7021 agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcaaccggg catttttggt
     7081 ttttattaaa tattcaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt
     7141 tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca
     7201 cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct
     7261 tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca
     7321 gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc
     7381 acacgctgtt tttgtccacc agaaagcatt tcaggataca tatctttttt atcttctaga
     7441 ccaactgtct gcagtaattt gattgctttt ttctctgctt cttctctaga ttctttcttc
     7501 aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag
     7561 tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc
     7621 aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa
     7681 cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg
     7741 ttgatggata cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc
     7801 tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt
     7861 tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact
     7921 ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc
     7981 ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat
     8041 gatgcgtatt gcttgtggca aaatgatttt ttgcattgtc cgattataag aaagacctaa
     8101 acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc
     8161 tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat
     8221 cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg
     8281 aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc
     8341 tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt
     8401 taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc
     8461 aagaaatgtt gattcatcag cagtggaaga ctcactagaa gtttctgaac catccgctac
     8521 atattctttg ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt
     8581 gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc
     8641 agattctttt tcgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac
     8701 cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc
     8761 agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc
     8821 agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt
     8881 gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga
     8941 aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc
     9001 ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt
     9061 aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc
     9121 ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc
     9181 ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt
     9241 gtcagcttgt gcaacatgac caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa
     9301 tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat attttattat
     9361 tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg
     9421 agtttctaac attttatgtt ataaaacgta acatgtataa taagataaaa aagtggcgaa
     9481 agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt
     9541 attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat
     9601 acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg
     9661 attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg
     9721 actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca
     9781 ctgattattg ctcataataa aacattggct ggtcagctgt acggggaatt caaggaattt
     9841 ttccctaaca atgctgttga atattttgtt tcttattatg attattatca gcctgaagct
     9901 tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac
     9961 aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca
    10021 tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg
    10081 attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa
    10141 ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg
    10201 gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag
    10261 gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac
    10321 gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta
    10381 gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt
    10441 ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg
    10501 ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa
    10561 ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg
    10621 cacgtaacca tgcctcaaat cagaggaatg tataatggtg accgagcgag aaaacagatg
    10681 ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa
    10741 gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa
    10801 catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca
    10861 gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa
    10921 cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac
    10981 ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa
    11041 acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt
    11101 ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg
    11161 gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga
    11221 gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg
    11281 cgtctagcga tggatgaaac gtcaagacgt cgtacgattc agcagaagta caatgaagaa
    11341 catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa
    11401 gaatcggaag atgatacaaa agaagcggta caagtctcgt acgaagaaat gacgaaggaa
    11461 gaaaaagaca cgttattgat gaaattagaa aaagaaatga aagatgcggc aaaagctttg
    11521 gattttgaaa cagccgcgaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag
    11581 gaggacaaca tggctaacga taaaattacg attcatggtg cacgtgccca taacttgaaa
    11641 aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct
    11701 ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag
    11761 agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc
    11821 atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg
    11881 tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta
    11941 ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg
    12001 gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg
    12061 gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc
    12121 cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac
    12181 aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct
    12241 cgcctatttg attctttaga ggcggccctg cgattagcag acggctatgc tctggttgac
    12301 gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt
    12361 actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca
    12421 gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga
    12481 acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat
    12541 cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa
    12601 gaattaccgg aagagcatca ggagatcatc ttgaatggga cagatgatct tttccatttc
    12661 cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg
    12721 aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt
    12781 ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct
    12841 ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa
    12901 gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg
    12961 attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta
    13021 actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct
    13081 acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga
    13141 ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc
    13201 aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatttgatt
    13261 gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa
    13321 gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc
    13381 cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca
    13441 gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca
    13501 ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg
    13561 caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa
    13621 agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat
    13681 ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag
    13741 gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt
    13801 gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat
    13861 gttccttgtg aagtatgtca tggtaaacga tataattctg aaacattaga agtgcattac
    13921 aaaggaaaaa gcattgctga tattttggaa atgacagtag aggatgctgt agaattcttc
    13981 aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat
    14041 gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt
    14101 gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg
    14161 acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat
    14221 gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat
    14281 atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca
    14341 ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg
    14401 gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt
    14461 gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt
    14521 atagataaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag
    14581 tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa
    14641 acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat
    14701 ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc
    14761 atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa
    14821 aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtattta tcgagacgat
    14881 gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg
    14941 tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat
    15001 ctgtccatat attgattatt ctttatttga aaggctgatc aataaagtaa gcttttatga
    15061 agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt
    15121 agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc
    15181 aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga
    15241 gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga
    15301 aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta
    15361 tgtagatgaa gatcatcaag ttcggatgat gtgccttggt catccactat tagaagatcc
    15421 tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta
    15481 tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat
    15541 gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg
    15601 tagcagtttc tttgtgacat tgaacggctt gaatctggct cgttttgtca cagaagatcg
    15661 agtgttcaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag
    15721 attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa
    15781 aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtat tttatgaaaa
    15841 agaccgatca atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag
    15901 attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt
    15961 aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa
    16021 agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga
    16081 tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga
    16141 tattgagaaa caaaatttat tgcgaccaaa cttcattgtg ttgacttgta acaccgcaca
    16201 ctactttttt gaagagttac aagcggcaac ggatattcct atcttgcata tgccaagaga
    16261 agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga
    16321 aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggtt tcgaaacagt
    16381 gattcctgat actgcgcttc aagaaaaaat caattacctg atctatcatg agatcaagga
    16441 atcggattat ttgaatcaag agctctatta cgaaatctta gaggaagcgg tagagcgcct
    16501 aaactgtgaa aaagtcatcc taggttgcac agaattatct ttgatgcatg aatttgctga
    16561 agataatcat tacccagtga tcgatgctca atctatttta gcggatcgaa cgatcgaacg
    16621 tgcattagct gaaagaagcg aggcattgga tacagcatcg gaaaagtaaa aaaattggat
    16681 ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac
    16741 tggtaatgat acgcccggga tgatataatt ttattatgca caatgtgaga ggggttcgta
    16801 aatatgacag ataatttgca attagtcatc ataacaggta tgagtggtgc agggaaaaca
    16861 gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga
    16921 ttgattccaa aattctggga attgatcaaa gaatcaggta aagtcacaaa aatggcttta
    16981 ggagtggatt tgcgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc
    17041 gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta
    17101 gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact
    17161 gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc
    17221 attgatacga caacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa
    17281 tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg
    17341 ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg
    17401 gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat
    17461 gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac
    17521 attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt
    17581 tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg
    17641 acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct
    17701 accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc
    17761 tgaaaagttt acgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg
    17821 gtggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa
    17881 atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt
    17941 tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag
    18001 tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg
    18061 tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtttttaaag
    18121 acggaacgac tgcagtagga gaatctaaaa tcgcgatcaa ccgtaaaacc attgaccgtg
    18181 tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag
    18241 caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc
    18301 caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct
    18361 atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata
    18421 tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg
    18481 aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg
    18541 cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttag
    18601 aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt
    18661 tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt
    18721 catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga
    18781 aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt
    18841 ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga
    18901 aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga
    18961 acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata
    19021 tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa
    19081 aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa
    19141 cgagtcgtta tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg
    19201 atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt
    19261 cgtatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact
    19321 caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc
    19381 tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg
    19441 aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg
    19501 aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga
    19561 ttccctcagg tgcaatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat
    19621 ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca
    19681 ggagatgcga atgtatcagt tttttttaaa agctatactt gcttttcttc cgtcaaacta
    19741 taattccaac tttactggag taacataatg ttgaaaggct tctattgttt tttaatacaa
    19801 tcgtataaaa agcggggaga caactgaatg aagattttag ttttatacat aaacttttgc
    19861 ttaatataga agtcctgata tagataggat caagtattta tctataactg gagattaatt
    19921 aaaaactgga aggaactgct cgatcaacct tgagaactgt ttttcttact tctggtcaag
    19981 attctcgtgg ttggtggata aggatggttg ataagttcat tggattagct agtcgaaaac
    20041 aaccaggtgt aattaatgta gaagttttaa caaggggatg gagtgattta ttcagtgata
    20101 atcataagtt cataaatagt aattgggatg tatggttggt atacgttata cctgaacttg
    20161 aactatcaag atttggttta gaatttgaga atcgatgttt ataaagggag ggcaaaaaat
    20221 gtgctttttt acaactttta ctatcattat gattgcagta ctttttattt ttctagatat
    20281 agcaaagcga aatacagcat ttttattata tcgggtttta cttcgagcag gtctcataac
    20341 atttatatcg atcgttggat tttttctatt cacagttatt gtatttattt ggagaactac
    20401 agcaccacca ttgccagaga ttacatatgg agaatttccg tttagtcttg aatatgaaat
    20461 agatgaggaa agaattttga ttgaagatac ggtgattgca gaatttatag gaagttttag
    20521 agggaatgtg acaactcaac ctagacgaag ttggagtata aaacttttaa ataatagtgg
    20581 agagttgaaa gcatttcatt atgaattctt aataatagaa agtgaagatg taacaatcac
    20641 tttaagtagt ggctctgctg gatattttat gaatgatggt ggaagcaagc cacagctcac
    20701 agtccgtgat cgtattgcag gttttatgca tttttctcac tatgacttag aagaagctcg
    20761 taaaatactt gcagaatatg ggatcacatt aatcaattgg gaatatgcgc caccgataag
    20821 aaattttttc aatgattatt attagtctta taaaaaacag aatgtaaaat ttatgtgcgc
    20881 gagtatttag ttttccattt tttgtttgat agatatatag cattgttgaa gcatgggata
    20941 gtggtgccaa ttttatcaat gaggttggtc aggcagttaa cataattggt gatgctataa
    21001 atccatttaa ttggaattgg aggaattagg tgtggaaaca gtgagattat ggatattcat
    21061 gaaaaatttt cgttcggctt tgagatatgt tattctggaa tcaaaatcag gttattatct
    21121 gattgacaca gatcactata ctttttgggg aggctacctt tttccgattt ttaattggat
    21181 aatcccccat aaggttatga aaattaactt gcctgcagaa gaaataaata gattaatact
    21241 caacaaagat ttacaaagga aaagagagaa agaacattct aatgttgctt ggataggtcc
    21301 agcatttgca gtagtgactt cttcaattgt cggtcctatg ctagagagta tttatagtga
    21361 tttttctatt ggaatacgtt atttatttgt tactttaagt atagctgttc ttttgtgcat
    21421 aaagggttat gttagcaaga agaataaaga tattttaaca ctaatcggtg taaacaattt
    21481 atctagtact aaaattatac tgtttcctga atctataaaa gaaggatcaa ggttgttgat
    21541 aatttttata atgcttatac tgttttctgt tttatttggg gtgatgttta ta
//
LOCUS       DAJSVF010000041        21503 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSVF010000041 DAJSVF010000000
VERSION     DAJSVF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.042"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            5..403
                     /locus_tag="ON550_001575"
     CDS             5..403
                     /locus_tag="ON550_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3954665.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ON550_001576"
     CDS             387..1082
                     /locus_tag="ON550_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR3954666.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="ON550_001577"
     CDS             complement(1666..2553)
                     /locus_tag="ON550_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3954667.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="ON550_001578"
     CDS             2738..3577
                     /locus_tag="ON550_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3954668.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="ON550_001579"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="ON550_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR3954669.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="ON550_001580"
     CDS             5045..5401
                     /locus_tag="ON550_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954670.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="ON550_001581"
     CDS             5404..6561
                     /locus_tag="ON550_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3954671.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="ON550_001582"
     CDS             complement(6587..7027)
                     /locus_tag="ON550_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3954672.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="ON550_001583"
     CDS             7187..7693
                     /locus_tag="ON550_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3954673.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="ON550_001584"
     CDS             7861..9774
                     /locus_tag="ON550_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3954674.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="ON550_001585"
     CDS             9779..10780
                     /locus_tag="ON550_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3954675.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="ON550_001586"
     CDS             complement(10982..11188)
                     /locus_tag="ON550_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR3954676.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="ON550_001587"
     CDS             complement(11834..12682)
                     /locus_tag="ON550_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3954677.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12681..13748
                     /gene="gnd"
                     /locus_tag="ON550_001588"
     CDS             12681..13748
                     /gene="gnd"
                     /locus_tag="ON550_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR3954678.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="ON550_001589"
     CDS             13750..14751
                     /locus_tag="ON550_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR3954679.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="ON550_001590"
     CDS             14748..15032
                     /locus_tag="ON550_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR3954680.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="ON550_001591"
     CDS             15065..15862
                     /locus_tag="ON550_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3954681.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="ON550_001592"
     CDS             15876..16709
                     /locus_tag="ON550_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3954682.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="ON550_001593"
     CDS             16727..17584
                     /locus_tag="ON550_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3954683.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="ON550_001594"
     CDS             17606..18115
                     /locus_tag="ON550_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3954684.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="ON550_001595"
     CDS             18152..18553
                     /locus_tag="ON550_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954685.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="ON550_001596"
     CDS             18665..19018
                     /locus_tag="ON550_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954686.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="ON550_001597"
     CDS             19052..19495
                     /locus_tag="ON550_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3954687.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="ON550_001598"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="ON550_001598"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3954688.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="ON550_001599"
     CDS             20590..21303
                     /locus_tag="ON550_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3954689.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7400 a         3301 c         4491 g         6311 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttc
//
LOCUS       DAJSVF010000042        21250 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSVF010000042 DAJSVF010000000
VERSION     DAJSVF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.043"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..115
                     /locus_tag="ON550_001600"
     CDS             <1..115
                     /locus_tag="ON550_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3954690.1"
                     /translation="LKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            112..870
                     /locus_tag="ON550_001601"
     CDS             112..870
                     /locus_tag="ON550_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3954691.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(956..1696)
                     /locus_tag="ON550_001602"
     CDS             complement(956..1696)
                     /locus_tag="ON550_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954692.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            1757..3010
                     /locus_tag="ON550_001603"
     CDS             1757..3010
                     /locus_tag="ON550_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3954693.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            3295..3558
                     /locus_tag="ON550_001604"
     CDS             3295..3558
                     /locus_tag="ON550_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3954694.1"
                     /translation="MEKQANPYDHKKMLTQQLRELEQDESIHRKAYNQVPPKVEYSLT
                     EEGKSLREILVALSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            3681..4007
                     /locus_tag="ON550_001605"
     CDS             3681..4007
                     /locus_tag="ON550_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3954695.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            4075..4788
                     /locus_tag="ON550_001606"
     CDS             4075..4788
                     /locus_tag="ON550_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3954696.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            5098..5949
                     /locus_tag="ON550_001607"
     CDS             5098..5949
                     /locus_tag="ON550_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3954697.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            6070..7935
                     /locus_tag="ON550_001608"
     CDS             6070..7935
                     /locus_tag="ON550_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3954698.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            7983..9392
                     /locus_tag="ON550_001609"
     CDS             7983..9392
                     /locus_tag="ON550_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3954699.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            9500..10201
                     /locus_tag="ON550_001610"
     CDS             9500..10201
                     /locus_tag="ON550_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3954700.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            10360..10839
                     /locus_tag="ON550_001611"
                     /pseudo
     CDS             10360..10839
                     /locus_tag="ON550_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            11687..12262
                     /locus_tag="ON550_001612"
     CDS             11687..12262
                     /locus_tag="ON550_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR3954701.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            12262..12702
                     /locus_tag="ON550_001613"
     CDS             12262..12702
                     /locus_tag="ON550_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3954702.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            12718..13419
                     /locus_tag="ON550_001614"
     CDS             12718..13419
                     /locus_tag="ON550_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3954703.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(13907..14332)
                     /locus_tag="ON550_001615"
     CDS             complement(13907..14332)
                     /locus_tag="ON550_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954704.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            14706..15677
                     /locus_tag="ON550_001616"
     CDS             14706..15677
                     /locus_tag="ON550_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3954705.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            15709..17106
                     /locus_tag="ON550_001617"
     CDS             15709..17106
                     /locus_tag="ON550_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3954706.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            17265..17888
                     /locus_tag="ON550_001618"
     CDS             17265..17888
                     /locus_tag="ON550_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954707.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            18637..20730
                     /locus_tag="ON550_001619"
     CDS             18637..20730
                     /locus_tag="ON550_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3954708.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            20890..>21250
                     /locus_tag="ON550_001620"
     CDS             20890..>21250
                     /locus_tag="ON550_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3954709.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEK"
BASE COUNT         7169 a         3502 c         4420 g         6159 t
ORIGIN      
        1 tttaaaaaat cagccagaaa catttgactg gcgatggctg atacttgttg gagtcattct
       61 tattggctta tttgctggat tatttatttt aaaagaaaga aggaaaaaga aatgaaaaaa
      121 acaattgcat taagtaccgt tctagctgga agtctattat tatgtacaca aagtgttttt
      181 gctgagaata agagctcagc cactacagaa ggaacgattt ctgtgaccaa accagatgct
      241 gcaaatcctg aagacgttgt tccagttcct cctgttgatc caaatgatcc tgatactcct
      301 tttacacctg acgaaccaaa tcagggaaca tccggattac tgacgattga ccaagctcca
      361 gatcttgatt ttggaacaat caagttaggt acagcaaaaa cagtatatgc tgcattacaa
      421 agcggaacgg acagtactgg agcacaaaca caagtacaga atttcgtaca agtaacggat
      481 aaaagcggaa attatgccgg ttggaaatta tcagtcaaac gaacagaatt tattggtaca
      541 acatctgata cgaataaact aactggttca aaggtgaaat tcaaaaacgc cgtgattcga
      601 aatagcgcaa ctaatagcag tggaatgcca tctaccgttt ctggaactgg cgctgaagga
      661 atcgaaatcc cagcagatca ggcagtagat ctagtaactg caggagcaaa tgaagggatt
      721 ggcacatgga tttattcatt aggagagaat gtggaacaag ggagacaatc agtggaatta
      781 tttgttcctg tatccaatta tgctgtagac aactacgttt caacattcac atggtcgtta
      841 acagatgctc ctacagaaga ggaagcgtaa aatcatatca acgatttcag ggagtggagc
      901 cgattcaagg cccactccct gaaatctgct atgcttacgt ctcagctctt attcatcaac
      961 tcgaagcagt tttttggaag tcatggaaac gttcctttgt cttttcaaag atctttcgac
     1021 caacgagtaa ggagtcaccg ttgaaaaatt ctaacacata atgagcagaa tcaattttct
     1081 gcaattgcga taagttgata cagtaagatt ttaaaagtaa aaagtaagca tacttttcta
     1141 actctgtctt caattgtttt aaagaagtat tcactaattt atcttctgta gttgttttga
     1201 ccataacttg gtttttcaaa cctttgatac tttcaatata taaaatttcc cgaggataca
     1261 ggacgagact tccacctttt tgatcaataa agattttatc gacatttacg atctgctgtt
     1321 ctttccaatt gatctgttct agcacttgac gaatttgttc agataaattc ctgtcttttc
     1381 tgatatatcc aatcggaagg attttccgat tgatgatctc tactgcaaaa ttcgtataag
     1441 cggtgaaaaa aacaatatta ctatgtgcat tttttttacg tatttgttcg gcgacatcaa
     1501 tacctgtgag tgattgcttc aagtcgatat cgataaagaa aacatcgtga ttatcaatag
     1561 tgcccaactg gatcaactga ttgatttgag agatagaagt gagctgcacg atattctcga
     1621 tatgatgatt gaccaattct ctgatgatcc attcgctgtg aaactggtta tcttctatga
     1681 taaaatactt tttcatatgc aggctcctat taaaaaaata tgtgtacatt ttgaaacaaa
     1741 acaatggagg aataagatgg tcgcaacagt gacactttat ttgaccttta ctttgatttt
     1801 agatttaggt atctatttat tcttttcgaa acaactgcta gtaaaaaaca gctttggttg
     1861 tatagctttg atggtggtga cacttttact tcctatccct aaagtgagct ttccttacct
     1921 agttggctta ttattgatgt ttctttttta ttttactacg aaaaacctgt ttatcagctg
     1981 tttgatcagt aatctttttt ttgctatcac tggactcagc ttttttctgg tttttgatct
     2041 gatggattat ttaggaaaga gacatcttgg aacagcaagt ttttttgcac tacttatcgc
     2101 atttattttc tatctattag taaaaagtat agacaaacag acgagatttc tcaacttgct
     2161 ttttacttat ccaactgaac actattttgg aggtatttgt attttattaa gctggggcgt
     2221 gatttacttg gtattgggaa gtgttcactt ccgctcatct aattatctgc ttctatctct
     2281 tttgtcgata gtcatcagtt ttttttcttt gtttttttcc attatgttga tcagtttcca
     2341 agtaaaagaa agacaacatc aggaatcgct tcaactgtat caaataaatg aagaatatta
     2401 ccatcatcta gaggaattca aacatgatta taaaagtcta ctgttttcat taaaaattac
     2461 tcttcaggaa aataaaaaag aaacgttgga atttttagaa atattagaag atcattctgc
     2521 agctattttt gaaaaaccgc aaatacaaca gctaacaaat attgtatccc cagctgtcag
     2581 gggtgtcttt ttaaaatttc tagaaaaagc agaatcggag aaaatcccga tccaagtagt
     2641 tattcctgtc gaagtgaaaa caatcccgat tgatctggtt gtttttattc gctgtttatc
     2701 tatttttatt tctaacgcat tcgatcatcg tgtagccgat cagagtccga ttacgatcaa
     2761 tctgctggaa gaaaaagagg gatcacgttt tagtatagcg aacaaggcaa atccaacgaa
     2821 tttgacgcta tcggagatgg tcaaaagagg aaaaacctct aaaaaaaatg gcgggttagg
     2881 tctatacagt gcgaaaaaaa tgttggatgt ttacgaaaat gcagaactaa aaatcgaatt
     2941 ttcaaaacag aatcactatt tttttgtaga aatgtatctg gcatctagaa agagcaaatc
     3001 agcctactga cttactgtca aaggataagt ttgaggaaag tctacacgga gatagagaca
     3061 acaacacagc atattcaaaa gaaacagaag cgtgatgacc gttgtcaccc ttctgtttct
     3121 tttgctatgt agttaaacaa agtagttgac acttccgcta cttagtatta gtacgaaagt
     3181 atacttcatg atactaagta gataagaagt gaaaagcgga acccacgatc gtcaaaatgc
     3241 taactgcttt gcttacacca gataaaggga agagcaatat cgcttatttt aggcgtggag
     3301 aagcaagcaa atccctacga tcacaaaaaa atgctgaccc agcagttaag agagttagaa
     3361 caagatgaaa gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt agagtactcg
     3421 ctcaccgaag aaggaaaatc attaagagag attctagtag cgttgtctgt ttgggaagaa
     3481 gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca cgctactgca tacacatggt
     3541 ggctacctaa attattgatt ttctatgaca tttattttaa ataaaagaaa acaggagttt
     3601 ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca agtaagaagt
     3661 gattggaaga aggcaagcaa atgaatagta tggaaaaagt caaccagttt agagatgaac
     3721 gtaattggcg acaatttcat aacgaaaaag atttggctat ctctatttct ttggaagcat
     3781 ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa acacaattag
     3841 aacgaatcaa agaagaactg gcagatgtat tgatctattc atacatgatg gcagacaacc
     3901 ttcacctgga tctagatgag atcattgaag aaaaattgat caaaaataat ttgaaatatc
     3961 ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa tgtgtcattc
     4021 ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa
     4081 aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt gttattagta
     4141 ggactagctt tgattttcaa tgaacagatc aaggattatt ttgtcagaga gacaggagat
     4201 aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga taaagatgtc
     4261 agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag gtcgcaaatg
     4321 agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc aatcaattta
     4381 ccggttttca aaggcttgga taatacgtca ctactttacg gagcaggaac attatctcca
     4441 gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa
     4501 ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt gacagattta
     4561 gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac agatacccaa
     4621 ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg cgacatggac
     4681 gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacatcgat aaaagatgcg
     4741 acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga tactgggaca
     4801 taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa aaccaatttg
     4861 ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt ggttgttttc tagatagact
     4921 tgtgttccag tttttttatt ttttatatga aaaggcttgc ataaataaaa attcgatggt
     4981 atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc agaattcagc
     5041 taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg aagcaaaatg
     5101 aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt cggggaagaa
     5161 gtcgtttgta tgggaaaagg actagctttt caaaaaaaga ttggggacga gatttcgcca
     5221 gaagcggtcc aaaaagaatt tgttttaaga gattcttttg ctaaaaacca attccagcag
     5281 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     5341 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     5401 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     5461 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     5521 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     5581 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     5641 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     5701 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     5761 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     5821 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     5881 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     5941 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     6001 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     6061 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     6121 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     6181 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     6241 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     6301 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     6361 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     6421 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     6481 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     6541 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     6601 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     6661 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     6721 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     6781 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     6841 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     6901 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     6961 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     7021 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     7081 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     7141 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     7201 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     7261 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     7321 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     7381 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     7441 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     7501 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     7561 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     7621 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     7681 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     7741 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     7801 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     7861 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     7921 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     7981 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     8041 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     8101 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     8161 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     8221 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     8281 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     8341 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
     8401 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
     8461 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
     8521 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
     8581 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
     8641 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
     8701 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
     8761 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
     8821 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
     8881 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
     8941 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
     9001 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
     9061 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
     9121 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
     9181 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
     9241 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
     9301 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
     9361 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
     9421 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
     9481 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
     9541 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
     9601 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
     9661 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
     9721 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
     9781 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
     9841 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
     9901 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
     9961 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    10021 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    10081 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    10141 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    10201 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    10261 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    10321 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    10381 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    10441 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    10501 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    10561 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    10621 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    10681 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    10741 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    10801 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    10861 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    10921 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    10981 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    11041 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    11101 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    11161 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    11221 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    11281 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    11341 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    11401 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    11461 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    11521 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    11581 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    11641 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    11701 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    11761 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    11821 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    11881 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    11941 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    12001 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    12061 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    12121 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    12181 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    12241 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    12301 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    12361 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    12421 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    12481 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    12541 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    12601 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    12661 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    12721 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    12781 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    12841 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    12901 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    12961 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    13021 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    13081 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    13141 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    13201 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    13261 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    13321 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    13381 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    13441 gaaagaaaaa ttactacgtg atgtagaata agcctttcta tcgtttggtt attttatatt
    13501 caaatatttg aaataaataa atctttactc tttgatttgt aaactgaaat gatccactgt
    13561 ttgttttact gatgaagctt gatcaaagga agaagctgag tttgaagttc ataaaaatca
    13621 gccatagtga gttttgcttg gatttgttta tcttcttcta ctttttacat aattttgaac
    13681 aggataagat tcaaatgttt taaatgaatc tttgactagt ttgtgacagt tgtcgcatat
    13741 gctcaagctt ccttggtcat taaattctgt atggatattt ttgttgttat tacatgtaca
    13801 ataatcagga ctatcgatca tccatttcgt ctcctttaca atcatcttgc ttctttacta
    13861 tagcagaaaa gaaggggaga aacaaaagaa gatccaacag acggcactat agactgtaga
    13921 tgttgccatc ctcatccttc aaacggtaat taccgatttt tgtaaatatg actgttgttt
    13981 cttttttatt ttcttttcct gaaataatat attcgtcatc tttggtagtg atcttgatat
    14041 ctgtcagcac tgttttttct tcagatgatt cagaagaatc agttccttgt gtctgatacg
    14101 tgcgtgtcca ttccattttt ttatctgtaa atgtcaactg tcccgcggat aaatccatat
    14161 ctttacgtac ttttagcgta tatacactgt tggtggtatc tgctttttcc gcttttaaga
    14221 cagaagaatc ggttgtttcg tccgaggatt ggtcagagcc gccaaacaaa tcacagccag
    14281 tcaatgtcaa aagtcccgca gacaaaataa atagtacgat taatttcttc aaaatacttc
    14341 ccccatttga taggataaag aaattatacc attggaatgt aaaacttcaa acggatggaa
    14401 gagaaaaacg cacaaaacac ccacaaatag agtaaatttt agaataaaat tttaaaaagg
    14461 caaaaaatcc gccactttca gaaagcgttt taaaaaagtg ttgcaatctt ttaaaaaaat
    14521 gctatattaa aaatgaaccg gttcaaatac ttgtttaagt ataattaata gtgagaggaa
    14581 gttgctctac tgaagacttt agttatatgg atattgatga caagcaacac tatgcaaaga
    14641 atgcggtcat cataatcgaa caacaagaaa gagaaatcaa atgtaactgg caggagtgag
    14701 aatgtatggc atcgatccgt gatgtggcta agatggctgg tgtttcagtg ggaacagttt
    14761 cccgttactt gaatggtcag caactgaagg aaaagaacat gaaaaaaata gcagaagcga
    14821 tttcagcgtt ggactataaa gaaaacatca ttgctaaagg gttgaagaat aatcgaagct
    14881 tctctattgg acttttgata aacgggattt ccagccgttt tgggtcggaa gttgtctcag
    14941 ggatcgaaag agtagctgaa gaaaatgggt atagcttatt attaagcggg tttgccgatc
    15001 agccagaaaa aattgaacaa aaaattgaat acctgatgaa gcatgtgatc gatgggctga
    15061 ttgtcttctt atccgaagaa gagtggtctg gatttgaaat gctcacaaaa atgagtattc
    15121 cagttatcgc attgaattgt ccagaaggac cagcaggtat tgatacaata ttagtgaatg
    15181 atcgagaaag tgtagcaaaa gtgatcgctc atcatggtga acaaggacat aaaaaaatcg
    15241 gatttattgc tgccactcaa acagactacg tagcaagaga acgtttagca ggagccaaag
    15301 aagcggtcca aaatgatcca tcgatccaat tagaggtctt tacaggagac tattctagaa
    15361 aaagcggcta ttacggggca aaagcattat tagaaaaagg ggcaactgct atttttgtca
    15421 gcaattacaa tatgtcgatt ggtgccatcg aatgtttcaa tcaagaaggt gtcagaatcg
    15481 ggaaggatat tgcgttcagt cactatgatt atttagataa aaacgatctg agtatccttc
    15541 cgaaaatcac gatcacgcca ccaacagata aaatcgggga acgagcagca gaacagctgt
    15601 taaaacgaat caaaaaagaa gaggaagaaa atgaaaaagt gattgtgatg gaaaatatca
    15661 tacatggaat caactaaaac gaactaaaaa agaaaaagag gtgctttgat gaaaaaatat
    15721 caatttccag caaatttttg gtggggttct gcagcaagtg gcccacaaac agaaggaagg
    15781 atttcaggcg atggaaaagg agagaacatt tttgattatt ggtacaaaaa agaaccgcaa
    15841 aagttctttg atcaagtagg acctgaaaaa acctcgcaag tttatacgaa gtatcaagag
    15901 gatgtacagt tgatgaaaca gactgggcat aattcatttc gaacatctat ccaatggagc
    15961 cgtttgatac ctgatgaatc tggaacaatc aacgaagaag ccgttcgttt ctatcatcgt
    16021 tattttgatg agttgattga aaacggtatc gaaccctttg tgaatctgta tcatttcgat
    16081 atgccgatgt atctgcaaga aaaaggcgga tggttgaacc gagaaacggt tgaagcctat
    16141 gaaaaatatg ccaaaagttg ttttgaattg tttggttcga aagtaaaaaa atggtttact
    16201 cataatgaac caatcgttcc agtcgaagca ggttatttat atcgatggca ttatccagaa
    16261 gagtcagata tgaaaaaagc gatccaagtt ggctatcatg aagcgttggc ttcagcgtta
    16321 gccatcaaat cttaccatga aatggagcaa ggtggtgtta ttggtatcat cttgaatctg
    16381 acaccaagct atccaagaga cgaatcaaac cctgaagatg ttaaagcagc gcaaatagca
    16441 gatgcgttct tcaatcgttc attccttgat ccagctgtaa aaggcgagtt tccaagtgaa
    16501 ttgattgaga tagtaaaaga acttgatctc atgcctgtga tccatgcaga ggatttgacg
    16561 gttatcaaag aaaatacagt agatctttta ggtatcaatt attaccagcc acgccgggta
    16621 aaagcaaaag aaacaccaat cgatactaaa aacggtccaa tgcctgagga ttatttcgac
    16681 aattatgaaa tgcctggtcg taaaatgaat ccttatcgcg gatgggagat ctatgaacaa
    16741 ggaatctatg atattttgat caatgtacga gatcattatg gaaatatcga ctgctttatt
    16801 tctgaaaacg gtatgggtgt agaaaacgaa gcacgattta tcaaagaaga tggcatgatc
    16861 cacgatgatt atcggatcga atttgtccaa tctcatctga aatatgtcca tcaagccatt
    16921 caagaaggtg ccaattgttt aggctatcat atgtggacat gtatggataa ctggtcgtgg
    16981 accaatgcat ataagaatcg atatggcttt atttcagtag acttagcaca agatggaaaa
    17041 agaacaatca aaaaatcggg tcattggttc aaagacgtat cagataaaaa cggatttgag
    17101 gcttaagaaa gaatagttga gagcaaatgc gtgaaattct ccattcttac cattttgtga
    17161 aaaagaagtt caattattta cgatcttttt caactttttt gtctttctat ttcatttttt
    17221 gtatataata gaatagtcaa aattaaatag gagtggagga aagcatgagg aaaaaaagac
    17281 cgtttcttct cttttggtta ttcggttttt tcttactgct tattttatcc gcagcatttt
    17341 caggaaaaat gacagcagat cttgctcaga aagaaacaga aaaaatcgta gcaagcaaag
    17401 aatcagtaga taatttcaaa aaagaattct caacttattg gtgggcggag tataaaataa
    17461 ctgccaatta cgaaaaaggc gaattggaca tttatttgcc aaaaggaaaa gaagaaaatc
    17521 aatttattaa tatcgatgac gtacttagtg cgatttctct cagacaatat caatttaaag
    17581 acaagcattt gaccgttttg cttttgactc atgatggcga caaagtagcc cgtttgaaaa
    17641 atggaaaagt gatcaccgct tattctgaaa aagtagacaa aaaagtggtc gccgatcgtt
    17701 tagcggaatg gaaaaagaca aatgaacaag tcaagaaaac aactgtctct tctcaaaaaa
    17761 acgagtcgac aggaacaagt ccttcagatt ctggcagtgg acaaacgata acgggaagtg
    17821 aagaaaatca agcctcaatt attacgaatg agtcatcaca aggacaatat caatcctatg
    17881 gacaatgaaa aacagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac
    17941 ggcaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    18001 aacaccgtgt atcaagaggt cttgagcctg ccgtttaact ctgagccaca aggagcaatt
    18061 tcaaaaaata gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt
    18121 ttgaacaccg tttaccaaga ggttgtaaaa ttagcgtcct gacataatct aataccgagg
    18181 gtcagctgat tgaacaccgt gtatcaagag gtcttgagcc tgccgtttaa ctctgagcca
    18241 caaggaacaa tttcaaaaaa cagcttctca tgttttataa atattgtgac ttgtggccga
    18301 ggagttggca ggagaacacc gtttaaggaa gaagttgtga aaaagctgtc ctgcatcaag
    18361 aaataaggag ctgcgacaag aattttgtca cagcctcttt ttttgtacga ataccgagaa
    18421 actggctttt gaataccgtg gagtgaagcg gtcaccacaa gacttttatc tcttcattag
    18481 tttaatcgct tacatcgcag cggttttatg ctgtgaaaag tggacggaat aatctttaga
    18541 aagaataaaa aaatcttaag ataacaactt ttttaggaaa ttttcatttt tcaggagcaa
    18601 aataagaaaa aatgtgaata gagagaagtg atatacgtga cacaacttaa aaaattgtgc
    18661 caaaatcgtt tagcaatcgt tttgacagct gttttctttt tctggctgaa gacgatcatt
    18721 gcctactatg cagatttttc attaggtgtg gaaggtacga tccagtactt tatcttgtgg
    18781 atcaacccaa ttgcgactac cttattgttt tttggccttt ctttgtatat taaaaagcca
    18841 aagccagcgt tagctattct tttgatcatt gatattttaa atacgctttt gttgtacttg
    18901 aatattattt tttatcggga attcacagat ttcattacag tcaaatctgt attaggcttt
    18961 tcgaaagtgt cgcaaggatt gtcaggaagc tccttttctt tgatgaagcc ccatgatgtc
    19021 atctactggc tggatatcgc tgtgttcatt gggctactgg tttggttgaa ggtcaagaaa
    19081 atacctatca aaagcaaccc tgtcggcaaa ccgatggcta tcgctgttac ctgtctttca
    19141 gggcttattt tttcaggaaa tctagcgctg agcgaagcca accgtccaca attgctgcag
    19201 cgaacatttg accgttcata tattgtcaaa tatctaggaa tcgatgcgta tactatttat
    19261 gacggaatca aaacaggaat gactagcagt gttcgcgcgc atgcaagcag taatgggatt
    19321 gatgaagttt tagattatac aaagaaacat tatgcagagc caaatcccga aacatttggg
    19381 atcgctaaag ggaaaaatgt gatcgttctc catttagaaa gtttccagca gtttttgatt
    19441 aatatgaagg tagacggaca agaagtcaca ccgtttttaa atagtatttt ccagaatcaa
    19501 gcaacgatca gctttgacaa tttctttcat gaagtaggac agggaaagac aagtgatgct
    19561 gaaaatatgt tggaaactgg cacatttggt ttacctcaag gctcattatt cacagaactt
    19621 gggtcagaca atgtctttca agccgcacca gcgatccttg gacaaaagca aggctataca
    19681 agcgctgttt tccatgggaa tgtagctagt ttttggaata gagatcatgt atataaaaac
    19741 ttaggttatg acaatttctt tgatcgatct tattttgatg aatccgacga aacgttaggt
    19801 tatgggatat tagataaaga tttattcaga gaatctgccc aatatcttga gcatctacaa
    19861 cagccgtttt acacgaaatt tctttccgta accaaccaca cgccttatta tacggatgac
    19921 aagaatttcg actttccatc tttgaatact ggcaatagca cagtagataa ttatgttcga
    19981 acggcacatt atctggatca atcattggaa caatttttta cgtatttgaa aaaatctggt
    20041 atttaccaaa attcaatctt tgtgatttac ggggatcatt ttgggatctc gaatacagat
    20101 aataaagatt tagcaagcgc tcttggtaaa gatccagata catgggatga atttgacaac
    20161 gcacagatgc aacgagtgcc attgatgatc catatgcctg gttatactaa agggacagtc
    20221 aatcatgaat acggtggaga aatcgatgtt ttgccgacat tactacatct attgggaatc
    20281 gatgataaag attatcttca ttttggtaca gacttattat cttctcagca tgatcaagta
    20341 gttgcttttc gaaatgggaa ctttgtgaca cccaaataca ccgtcctagg aggaaaagct
    20401 tacaataatc agacaggaga aatcatcgat acgaaagcag caggaatcga tgaggaaatc
    20461 tccaaagatc aggaaaaagt caagactgcg ttaagtcttt cagataagct gaatcaggaa
    20521 aatctcttgc ggttttatgt gccaaacgga tttgagacga ttgatccgaa aaaatatgac
    20581 tataaaaatg aagcggaacg taatgaagca atcgaaaagc aaaaaggcga acattcgaca
    20641 agtgtctttt caaaaaatgg taataaaaca acaaccaact tatatccagt aacaagaaca
    20701 gacgttgaac agcaaaaaaa taatcaataa acaatcaaac caaccttcag tagttagaca
    20761 acattctaat gcttgaaggt tggtattttt tatgaaaaaa acattttttt atttttttcg
    20821 tgtatttttc ctttttaacg tatctattta ttgaatgtgg aaaaatttaa attaaaaagg
    20881 agaactatta tgtatgatgg tgaattgatc aagaaacttc gagtcaacaa gaagttgaca
    20941 cagtcccaat tagcggaagg aatctgtttt aagacttctc tagtcgggat tgagacaaat
    21001 tcagtgaaaa aaatgtcatt tgtaacattg agatcatttt tagagcgaat gaacatgaca
    21061 cttgcagaat atgaatggat gcgaaaccag ctggatgaac caataaagtt aaagaaaaaa
    21121 agacgaatgc tagatcgggt acaagaagac agctttgatc cttataaaga gatcagcaat
    21181 aaccgtaggc attttaagaa aacagaagat ctgtattatt tggttttgaa tcttcacatg
    21241 gtcgaaaaaa
//
LOCUS       DAJSVF010000043        20362 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSVF010000043 DAJSVF010000000
VERSION     DAJSVF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.044"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            292..1431
                     /locus_tag="ON550_001621"
     CDS             292..1431
                     /locus_tag="ON550_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR3954710.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="ON550_001622"
     CDS             complement(1498..2073)
                     /locus_tag="ON550_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3954711.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="ON550_001623"
     CDS             complement(2078..3052)
                     /locus_tag="ON550_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR3954712.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="ON550_001624"
     CDS             3335..4303
                     /locus_tag="ON550_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3954713.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="ON550_001625"
     CDS             4416..4694
                     /locus_tag="ON550_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3954714.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="ON550_001626"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="ON550_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR3954715.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="ON550_001627"
     CDS             5151..5906
                     /locus_tag="ON550_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3954716.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="ON550_001628"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="ON550_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR3954717.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="ON550_001629"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="ON550_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR3954718.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="ON550_001630"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="ON550_001630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR3954719.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="ON550_001631"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="ON550_001631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR3954720.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="ON550_001632"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="ON550_001632"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR3954721.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="ON550_001633"
     CDS             10643..11185
                     /locus_tag="ON550_001633"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR3954722.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="ON550_001634"
     CDS             11208..12764
                     /locus_tag="ON550_001634"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR3954723.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="ON550_001635"
     CDS             12778..13680
                     /locus_tag="ON550_001635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR3954724.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="ON550_001636"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="ON550_001636"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR3954725.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="ON550_001637"
     CDS             15123..15545
                     /locus_tag="ON550_001637"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR3954726.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="ON550_001638"
     CDS             16014..16244
                     /locus_tag="ON550_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR3954727.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="ON550_001639"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="ON550_001639"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3954728.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="ON550_001640"
     CDS             17655..17831
                     /locus_tag="ON550_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3954729.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="ON550_001641"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="ON550_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR3954730.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="ON550_001642"
     CDS             18326..18709
                     /locus_tag="ON550_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954731.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="ON550_001643"
     CDS             18878..19762
                     /locus_tag="ON550_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3954732.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="ON550_001644"
     CDS             19876..20112
                     /locus_tag="ON550_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR3954733.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="ON550_001645"
     tRNA            20200..20280
                     /locus_tag="ON550_001645"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="ON550_001646"
     tRNA            20287..20358
                     /locus_tag="ON550_001646"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3566 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta ta
//
LOCUS       DAJSVF010000044        20216 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSVF010000044 DAJSVF010000000
VERSION     DAJSVF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.045"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            42..281
                     /locus_tag="ON550_001647"
     CDS             42..281
                     /locus_tag="ON550_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954734.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            348..587
                     /locus_tag="ON550_001648"
     CDS             348..587
                     /locus_tag="ON550_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954735.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            673..1344
                     /locus_tag="ON550_001649"
     CDS             673..1344
                     /locus_tag="ON550_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3954736.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1397..3373
                     /locus_tag="ON550_001650"
     CDS             1397..3373
                     /locus_tag="ON550_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3954737.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDNVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3386..4141
                     /locus_tag="ON550_001651"
     CDS             3386..4141
                     /locus_tag="ON550_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3954738.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4138..4962
                     /locus_tag="ON550_001652"
     CDS             4138..4962
                     /locus_tag="ON550_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954739.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5264..7363
                     /locus_tag="ON550_001653"
     CDS             5264..7363
                     /locus_tag="ON550_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3954740.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVLDGKNYDGALWYVEANGTWKKADTGGKLTVSSNTVTYTASDSFLANA
                     ANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGTQRTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7401..9458)
                     /locus_tag="ON550_001654"
     CDS             complement(7401..9458)
                     /locus_tag="ON550_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR3954741.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9572..9784)
                     /locus_tag="ON550_001655"
     CDS             complement(9572..9784)
                     /locus_tag="ON550_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954742.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9971..10438
                     /locus_tag="ON550_001656"
     CDS             9971..10438
                     /locus_tag="ON550_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954743.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10435..10677
                     /locus_tag="ON550_001657"
     CDS             10435..10677
                     /locus_tag="ON550_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3954744.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10695..10997
                     /locus_tag="ON550_001658"
     CDS             10695..10997
                     /locus_tag="ON550_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954745.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11068..13122
                     /locus_tag="ON550_001659"
     CDS             11068..13122
                     /locus_tag="ON550_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954746.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     TNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13122..15734
                     /locus_tag="ON550_001660"
     CDS             13122..15734
                     /locus_tag="ON550_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3954747.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNM
                     HDQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVI
                     RKRLLLEAEKAVKE"
     gene            15731..18262
                     /locus_tag="ON550_001661"
     CDS             15731..18262
                     /locus_tag="ON550_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954748.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18312..18644
                     /locus_tag="ON550_001662"
     CDS             18312..18644
                     /locus_tag="ON550_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954749.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18645..19238
                     /locus_tag="ON550_001663"
     CDS             18645..19238
                     /locus_tag="ON550_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954750.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19401..>20216
                     /locus_tag="ON550_001664"
     CDS             19401..>20216
                     /locus_tag="ON550_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3954751.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLL"
BASE COUNT         7291 a         3149 c         4040 g         5736 t
ORIGIN      
        1 cttaggcatt gagtaaggaa tttatttagg aggaaaatta gatggcgcga ccagttttag
       61 aggcgatgga tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt
      121 attcacctaa agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat
      181 tacgaaaaga attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac
      241 ggccagtaat taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat
      301 cataggcttt tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag
      361 aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga aaagttgcta aaaacgatga
      421 aacaaaaaca ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag
      481 gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt
      541 atgtgaaaaa aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag
      601 aattcatttt taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa
      661 ggaggtagct ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt
      721 tgcagtgtgt ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat
      781 atagaaaaaa agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat
      841 gcaacgaacg tcaataatga tatgtctttg gggcaaatgt tgtccataca aaaagaagca
      901 atcgaaatgg gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta
      961 gcattgccaa tttttaaagg tgcaaatcaa tacactttga gtcttggagc ggctacttat
     1021 ttttatgaag atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa
     1081 atgccaggtg ttttattttc tgatatccaa aagttgtcat taggggaggt gatggactta
     1141 gttagtaacg atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgggtac
     1201 tttaaattaa tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga
     1261 gagaaaccct tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac
     1321 gtggtacagg gagagctaca ataacaaggt tttaatcaac gcgagtcgtt gaaatatgaa
     1381 atgaggagag taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttgttgg
     1441 aatgttagga atcattgcag gctgctttca ggggtatcaa gtacatgcag aagatgtcac
     1501 gcaaaaaaca ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg
     1561 aacgcaattg aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata
     1621 tcctacaggt gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg
     1681 aaatattacg gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga
     1741 cgagattgta caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac
     1801 caacaaaatt acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc
     1861 ctatgtcaat tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt
     1921 agtcacacaa aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc
     1981 aggattttta aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga
     2041 attaacgaaa tttggtgacg atggcacagc tcaaagcaaa caaacacctt taaaaggggc
     2101 aaaatttgag ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc
     2161 ggatgatcaa ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga
     2221 agttccttcg gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt
     2281 aggcgcagac gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg
     2341 cgtgacaagt gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac
     2401 agtaacaaat ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca
     2461 aggaacgatt catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg
     2521 tgtgaaaagc gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaagg
     2581 actttcttat gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt
     2641 attaaaagaa aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt
     2701 tatcgtgaat aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca
     2761 aatgacttat aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag
     2821 cgtggatttc gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa
     2881 agctgacgtt gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac
     2941 aggaatcaaa gcaacaccgc ttgaaggcgc agaatttgct gttaaaaacg cagaaggaaa
     3001 atattatggt ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga
     3061 caatgtagca aatgcagcga ttctaaaatc agataaagaa gggcactttg aaatcactgg
     3121 tttaactgaa ggtgaatatt cattggaaga aacaaaagcg cctgaaaact atcaaaaatt
     3181 aacagaagat attgcattta aagtaaacaa aaactcattt aaagaggaaa atcgaatcac
     3241 tgttaaaaat aatcaaaaag cagctattcc attaacaggt agtaatggtt tccaaacgta
     3301 tgtattgatc tcttgcttgt tgctaggagc agggggactt tctgcagttg tttactttaa
     3361 gaagaaagcc tagaaggatc agctaatgat tattcgtaag cccccaaaaa gaagagtcat
     3421 gcacaaactg atgatatttt tttggttgct gtcggcagta gcagtctatt taattccagt
     3481 tgtggaaaca aatgtagcga aatttcaaca acagcaaaga attttagcca ttgaaaatct
     3541 tgaaaaaaca gattcttctt tggatgttgc gaaaatagag ttaggcgatc ctgttggtgt
     3601 tcttacaatt ccaagtattt ctttaaaact accaatttat gacggaactt ccgacaaaat
     3661 tttagagaat ggtgtaggga ttacggaagg aactggcgat attacaggag gaaacggtaa
     3721 aaatccttta atcgcggggc acagtggact ttataaagat aatcttttcg atgatttgcc
     3781 ttcagtaaaa aaagggcaaa aattctttat taaggtaaat gaagaacaac atgcctatca
     3841 aatttatcga attgaagaag ttaaagcaga agaattacaa aaaaattatt tagattatct
     3901 tatgcctgaa aaagaccaag ataggatcac gttaatgaca tgcacaccaa aaggtgtcaa
     3961 ctcacaccgc tttttagtgt atggtgaacg agtttccttc agtaaagaag atttaaaact
     4021 acagaaaaaa gcaaaaaata ggaaatcaat ttcattggat tggcgattag cactgctatt
     4081 cgcttttttt tgcgttccta ttatttttat ttataaaaaa ataaggagga gatcggaatg
     4141 aataagagaa aatggctgtt tgtttgtgta gcagctctct tttctactgt agcttattcg
     4201 caactgtctt tcgctgacag cagtgatagt agtaccactc aaacaataga taattcaatt
     4261 actgcaccaa gcgaaacgga cagttcggag aatactacag aagaaacggc agattcaaat
     4321 ataagcttta ctgaatcaac gaatccaacg tcagaaagtt cttcagagac ttcagaaaag
     4381 ccgcaagacg tagaagatac gcaagtgatc aagcaagcca ttcatattca aaaaattatt
     4441 tcttcgaatc aaatcaacaa tcaaggcagt caagtagccc agcaagaagg tatcaatggc
     4501 gcaaagttta ttgtctacga tatcacgaat atcttagaag aaatgtcaca agatgataat
     4561 gagaaaaatc aacctgtaga aataattgag tcaaaattga aagatcgagc aaaaaaactt
     4621 tcatacgacc agttgaaaaa agtggctgaa ggagaaacca aaacgattga tgatcaggaa
     4681 ggcgtgattg aattttccat tgaagtacct gcaaacaaaa aacaagcata ctatatcgtt
     4741 aatgaatcaa gccctgaaaa catttcaaac agtgaagata ttgttttatt gacacctgtt
     4801 tcaaatgaaa aaggtgaatt tttgacggat gtttggttat accctaaatc agagaaatca
     4861 gagccgaaag aagaaattaa aaaagttgtg tcaactggtg taaagaagaa cttttttgaa
     4921 aattgttggg aattttttgc tcaattattt tttgataatt aggatgaaat gatgaaaata
     4981 ataagaatat tagtttttaa tctatccttt ttagccgcaa cattattgtg ttgcggttta
     5041 attttttctc aattatcaat ttatccaaga tatcaacgat tgttcattca ttttctaaac
     5101 attcaatgtg cttccatgcc attagaggtg attaaaaagc tcttgatcag tccaattttt
     5161 atggttagtt tggttcttct tacaggattg gtaacgtggg tattaggaaa aaaagaatga
     5221 aaagcttacg atcgtttaaa aaaaatatta gatggagggc attatggaaa ataaaattca
     5281 ttgcactacg ggaagagtaa ttactttagt gttgtttttg ttccttcttc tttttgcgag
     5341 gggaactgca gtccaggcct tagaaaaagt tgatccgagt caaatcaaag aaaataataa
     5401 tctggtgctt tatcatgata gtgattcatt gagaaatatc gaagtaactg ggatcaatgg
     5461 atttaagata ctagatgagt attcaactaa aaaacaaaaa gtacttgctt ggccaaaagt
     5521 caaagatttg aaaagtacgt caaaaatcgt tgcccattat caaaaagtag gtacttatct
     5581 aggaaaagaa attgattgtg acgtgacttt ttctaatttt gtagcgcacg ctggggttaa
     5641 ccaagacgga ttagctaaaa caattaatgg caaaaagtat tataattatt tgctctttga
     5701 taaaaatatt tatcgaggat ttttcatgca gtcaatcaat gtcatgaatg caaaactaga
     5761 atttaagtat gcaggaacaa aaacaaaagt agctttgagt gaaccacagc cagaaattca
     5821 atcaaaaatg agatcgatga gagcagctgg tattgattta aacgatacat ttctatccat
     5881 ttatagttta aatggacagt atcagcaacg tgtgtcttgg tataatgaca ataatgaatc
     5941 tgtcggtgaa cgtaatattg atatgagaga atttgttggg tatgaaaaaa tgggtagctt
     6001 accttatttt gtcacaacag atacagcatg tgcagaatac aaagctcctg cgttatcaac
     6061 aaacaattta acttcaaaag tagtgggagg acgtgcagaa aaggcttata gctcgaatga
     6121 tcatttcacc gatgttgtag gagctgatac ttatcacaga agtggtgtaa cgtatacgct
     6181 tcaaggcgct tccccaacat tcatgattgg cgcaaatacg aatagtatga tgtttagctt
     6241 tgatactgca ttgctatgga caccacaacc atcgaagcct acaaaagaag tgtttaacaa
     6301 agctaatact gaagaggcag cacacaatat tgacaaaaaa gtgattccac aaggatcaga
     6361 tgtttactat catattcacc aaaaatttga tgcattaaca gtcaacacaa tgaacaaata
     6421 caaatcattt aaaatcactg atacctttga cagcaaaaat tttgatatgg tattggatgg
     6481 gaaaaactat gatggcgcat tgtggtatgt tgaagcgaat ggtacttgga aaaaagctga
     6541 tacaggagga aaattaactg tatcaagcaa tacagtcact tatactgctt ctgacagctt
     6601 tctagctaac gctgcaaatt acggtagaga ttatattttg cagctccata tgaaagcaaa
     6661 aaatgatgca aaaggaatcg cacccaatgt ggctactgta acgtttaaca atgatccgaa
     6721 taaagggaca caaagaacaa atacagtaga aaattatttc tctcttaaac caaataaaaa
     6781 agtggaacaa gatgggaaag atgttgatgg tcgaaatgat ggtaaaaaag gaacgcctac
     6841 agctccttta aatgcaggat ctgaagtaca atatctagtc acacaaaaat ggcatacaaa
     6901 aggtgtagac attgcgctcg atcattacaa gcaatttagt attcaagatc cagtggaagc
     6961 tcgtttgacg tataaagaag gttctgcgcg agtgatagat atgagtacag gaaaagatat
     7021 tacttcagaa ggtacactaa cctatgatag caattcaaga acgctgaagt gggaagcttc
     7081 cgctgatttc ttaagtaaaa atcttttaga tggacgagaa attcaactga tatttacagc
     7141 taaaactcct ttgaatgaag aattggatat cgacaaccaa gcaaaagttt cagtagaaag
     7201 tattgagaaa gcaactaatg tagtaacgat tggggtaaaa cctaacttac ctcagatcat
     7261 tgtgccaaaa actggatcga atcatttagt gctaactgtt attggaagta tattatttct
     7321 tactttagca tcattgggct atgtagtttt aaaactcaaa taaatataaa aacaaggctg
     7381 agaatcagcc ttgttttctt ttatttgaag attggattcg cactaataaa tacattatct
     7441 acgccaattc taccagcaga gtcaactaca aaattatgac ctaatccgag aatactttgg
     7501 ttattataaa gtaaatttgc attatgggca gggctatttt tccatcctac catggcatcg
     7561 atagcaccat cgtctgataa aaggtcatca atatacagcc cagtttgact tatgttttct
     7621 cccttagcac cgtagcagcc taagctttcc aataaatctt ggaaagtacc accaccaggg
     7681 cgatagtgac tgaagctgac ttgtaattct ttagctcgaa tatctgcagc ctgttgaaca
     7741 atatcattga attgcattgg ggcaacacca ttttgttggc gataatcatt taaccaagca
     7801 agttcttttt gacgcataaa gttacgtaat tcgtctaatg aaatcttttt atatttgaat
     7861 gtgattgttt tattttgatc gacgttatta atggatcgtt ggccgttttt atccacggta
     7921 acggtaggat cgtcatttct gcacaagaaa taattttgat cagtcatgtt tttccaagga
     7981 gtagcaaagc tatccccttc gtatactgtt tttgattcac tagagagaac agcgttggta
     8041 tcagcattta catgattaac agtcacatta aatttacgag tgtagttgaa aatataattt
     8101 tgactttcag tcacattgtt taaggttact gaatttgttg gttctacccg atcgtctaaa
     8161 acatatcctt gaatagaagg agcagttaaa gtgacactgt caccgtgagt gacattgact
     8221 atctgttctt tcaaaatatt aactcgtcct tcgtttttgt agttgttatt gcacaacaaa
     8281 ttgatttgat gcacttgatt ataattgaat gtgattgttg tattacctgt gattgttttg
     8341 ctttgagtat cattaccaat cactttgtag ctaaaattat ctttagcgcc ttcgctatat
     8401 ttaaatgctt ttgccttagc agtgtaagta gaaccagaag ttacttttgc ttgatctgta
     8461 gcaatcactt gacctgtttg attgtcaata tggttaatag taaccgtata ttcttttggt
     8521 gtttcaggtt tgtgttgcaa ccctttaaat gcagcatcaa gactattttt agcattattt
     8581 acttgggctt gagaagcctt agaatcgtct agtacttttt tagcattgga taaagcagtc
     8641 tgaaaattat tccaagattc ttctgtaaaa tctcctttag cagttgattt tactcggttg
     8701 tataaagctt gtaattcagc tttattcaca gtaggaactg gttttttctt taatcctttg
     8761 taagcagaat ctaatttatt tttggcacta tccacttgtt tttgagtcgc ttttgagtca
     8821 tttaacactt tcttagcatt gtttaaagcg gtttggaatg cgttccaggt attgtcggta
     8881 tagtcacctt ttttagttcc cttaacgctc gtataaagag cttctaaagc tgatttattc
     8941 actttgggtt tagaggtgtc agcgccatac caaccgatac cttcgtattt ccagccatag
     9001 tctttggtta accattgtac ttctttccaa cttgcagtgt agtgatgtga gccagatttt
     9061 acaccaggaa ggaaaaggcg gtagatcggt ttattatttt tttcggcaga acgccaggct
     9121 ggcccttcat aagtccagcc gtaattttta gtcaaccatt tcacttcatc ccaactagct
     9181 gtgtaatggt gatcacgaag acctttattg tacaaacgat agacaagatg gccaccgcta
     9241 gtcggtgctt cccaggcggg gccttcataa tttccccatt ttatctttac tagatggtca
     9301 cgttcttttt tgctagaggt gtaaaaatgt tcatgattat ttggattgta aaggcgatac
     9361 atgacgttag ttgaagcagc gtcaacagat ccagcaagac taagtgaggc agcaaatgtt
     9421 ccaatagcta aaacagtttt tggattaaat aatttcattt tttcaactcc taaatttaat
     9481 taaaaatgtt tacatgatta ttatatatcg gctaaccgat atagtcaata aaaaataaga
     9541 aaagccttat ataattttgg cttttcttat tttatttgtt agttgaagta ttaatatcaa
     9601 cgatttcttc agccaattta tttaatttca tagcatttat gaatctcatg ttgtctatac
     9661 tagagcgttt gttggcaagg tcacttactg tagttaaggg tacgccagat tctatggata
     9721 tagaacgaac tgatttacca ctatcaaaca gcaaccattc gattttcttt ttatcagcgt
     9781 acatttcaat gctccttctt gttttttctt tattatagct cttttttgta ataaatcaca
     9841 atatcaagag aaaaattttc tatagaaagg tggattttta tgagtattcg ttctccgtgt
     9901 atccaaatga ggtttgttgc tactttcgtt atttgatata ctaaaaataa acaacgaaag
     9961 aaggaataaa atgaagaaaa agcaactatg gtttgtctct atgtttgcaa tattttttat
    10021 tattttaagt ggatgttcaa gtaataagaa atcggaagct aaagaagata atagtttatt
    10081 gcaaccagga actgtttgga aagaagaagt aggtggtctt attcataatt taaaaattat
    10141 agatgatact acctgggaat attctgaaag tgtatggcat ccagatcctg tacaaataac
    10201 agttaagagg caaaaagact atgaaggttt agaaagatac aaaatagtag attctgcagg
    10261 tattagggaa tttataaaca aacctgattc attatttatt gttgttccct atgagaagga
    10321 tggcgtaaaa aaaataattt ttcttggatc tagcaaggat gagaagcgaa cgaaagagaa
    10381 attaattcat gatggatcag agttaaataa atacaaatta caaaagacta gtgagtgatt
    10441 gctagtcttt ttatttatcc tgaaggagga atcacagtgg caaaagttaa aacaccattc
    10501 gaaaaagccg cgtctaaaat cttagaacgc cgtggtgttt ctgaaataca gtggaaaaaa
    10561 gaagttcttg aaaaagcaca atttgaccta ttacgaggaa aagataagga cttagaagaa
    10621 tatatctatc aacgtgaacg tgaaaaactt gtattcgatg aaattattaa acattaaaag
    10681 gagcgaataa tcttatgttt gaatcaatta caatgaattt tttaactcaa ccgaaagtag
    10741 tagctgcagt tgatattttt agagcattac aaactgccgc aactagtttg aaaaataatc
    10801 tgatgatttt tgctgctgtt gcggcagtgg cttccattgc tcttggaggt attttcttct
    10861 tctttgggcg aagaggagct gaaaacggga agacacacgt aggttatgta ttgttggggg
    10921 taggagttat tttagctgcc gcttctattg tagtttcaat gttctcaatg tttggacaaa
    10981 ccccacaagg actataaaaa aacaagtaga aggagtaaaa agtacgtggc tactttttac
    11041 tccttttttt atggaaggag aatattcatg ttttttacga tttcaggcaa tgcaaaattg
    11101 gataaagagt taataagaaa agcaacgaag aaagtgggaa agcgtacttc ctttttaccg
    11161 aaaacaatta aatattctgt cattgaaacg gaagaacagc tacatgtgtt aggacaagtg
    11221 ttagaacaag agaatcaatc ttctgttaaa ttgactctca cgttacatga taaagaagga
    11281 aaaatagaaa aggctggcac agaatacgtt tttggctcac tggattatca aatttcagaa
    11341 ggtttttatc ctacattgat tgatttaatc cgcctagata tatttaaaga tacggatgaa
    11401 accatgagtg tggaagagtc ttatcgaaac gaaaaaattt tacaagtttt agaagaagag
    11461 ttagaaagcg accaaagaaa agtgaatcaa gtgagtgaag tcgaagcaaa cgaagaagta
    11521 ccatgggaac gcccaatctt tccaaaggat gaatcaaaag aaaaagagaa aggaattatt
    11581 gacgaacaag ctgacatttt gactcataaa tcaatatttg aggaagagaa cgaggtaatg
    11641 ggtcaattgg aggtagatcc ctttgaagaa acaatggagg atgaagagac ggccgaagta
    11701 gaagttgatc cagttgaagc taatgaagac ccaacatgga ctgaagggga ttctcaggaa
    11761 tatattgaac cacttatcag tgaagaagta gagaaaaatg aaacagagat acaaaaggca
    11821 acggtatttg attatatttt ggatcaatat ccgccagagc atgaatggat gaaagaaaaa
    11881 aataaattat ttttaaatga actttattcg aaatatcaat tacctcaaac ctatcaagca
    11941 tttttagaga gtaaagaaga attaatctca cagggtaaag gcacactttt agaacagtta
    12001 gagtctgtcc aatcaacaaa ttggcatgag cttgcacaag aagatttatt agctactttt
    12061 gaagaacgtc aacaagaaac agatcaagaa attaatgagt atgcgacgga acagtcgtcg
    12121 aattgggaga aagcaaaaga ggaactaaat aaagaagaac agcgagtgat tgaagaagaa
    12181 gtacaaaaaa ttaaagagcg gtacgaaaag aaacgtgaca atgcgtattc cttgtgtgta
    12241 gaaaagatca acaattttac tcagacgaat caagaagcat tatcgaaaga aaaagaaaac
    12301 ttattaaaag aacgtataaa agaacgaaaa gaagaatttt accgagggtt gcgtactgat
    12361 aagctaagga ttagcaaaga attaaatgaa caactgaata aactctatca aactacgact
    12421 gaagttctga tggaaaaaca cgagcagatc caacaagaat tacaagcaca aattccaaaa
    12481 tggaaaaaag agtatgaaat tcaccaacaa gaattgaaag aacaagaaga aaaacaacgg
    12541 aaaaaagaaa aagagcgcga acaattagaa ctaaaaaaac gtgaacagga tcttgcggaa
    12601 caaaaaatgg cgatagaaaa acaacggcta gagcaagaaa aggaaaaaga agcagctgct
    12661 cgtaaggaac gtcaagaaca aatggataca ttgcgacaag cacagttaaa ttatctgaca
    12721 catcctcaac cagtagctgc tgttcaaacg ccagtgaaca accaaacaaa tctaactcct
    12781 tcaccccagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
    12841 ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct
    12901 gtagccacaa cggcgtcaat caaggaagaa gcccaggaac aagcaagaaa agagtatgaa
    12961 caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagagtc aagcatggaa
    13021 aatagcaaag agagtgattc tagtaaggct aaagaaggat caagcgattc aactgtacaa
    13081 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
    13141 cgaagcgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
    13201 gctatactcc ctcaggagaa gagctaccag aaaaacatga caaacgcatt tcgaaaaaga
    13261 ttttttatag tggacttgta tacctccctt tagagtattt agtaacaaga gtaccagaag
    13321 ttttcaaccg attggaaatt gttaagggaa gcttacaagg aataaatctg tttcaattac
    13381 aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca tttactccta
    13441 caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca gctgtactta
    13501 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tggacaaaaa ggggataatc
    13561 gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa acaaaaacta
    13621 cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg tacattcaaa
    13681 cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt cagattgaag
    13741 tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg gtcgttaatg
    13801 atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga gggtatgatg
    13861 tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
    13921 tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc aatactttca
    13981 caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac gaaaacgcgc
    14041 agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa acaggagatt
    14101 actccaaatt gacaccgaat aatctcgttg agcttctttc tgagattggc ggttttaatt
    14161 attatccaga tccagacaat gaattcaaac agttgaatgc cttagatgaa tttatgaatc
    14221 agttgccacc tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt ggatctgata
    14281 aagcgaaggg aaatatttta tcaacggcaa tcacgggatt gaatccattt actctaacga
    14341 aaattgcaaa gatgacttct caatcaacga ttgattataa atcggttggg tttccaaaat
    14401 acttagattt gaaattacat gaaagtcttt taaatcaacg agtcgtagta gaattttatc
    14461 ataagggaaa gaaatttcat gaagaacgtg tcaaagtagg ctataaagga ttttgtgaaa
    14521 tcaattttga ttgtcattta gttgcaggtg atcaagtttg tttaaaatac gatcatcaag
    14581 gaaaagaata tgtggcttgg tatgaatttt ctcaaagagt attgaaagac gaagagggta
    14641 atatcgttta tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa aaagaagccg
    14701 ttctaacatt agatgaggaa aaaagtaact tattagtaga gtctattcgg atgcactata
    14761 ccgataaacc aatggctatt tttatgctta cacctgatta tgatccatct aaccatatca
    14821 ttgttagtat ttttcttagt cagttatata aagaattatc tacgcaatgt gttaagacta
    14881 aaggagataa atgtcaccgc aggattcatt ttattttaga tgaatttggg aatatgccac
    14941 caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg ttgtgggatt
    15001 tgtttatcca atcctatcag caactttatg cgaagtatgg ggaacaaaac gcagcgatta
    15061 tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agacgataat accatagaag
    15121 aattttccaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat
    15181 taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgcatctta actcctgaaa
    15241 gaatttctac ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt
    15301 tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt
    15361 atgcttatca atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac
    15421 attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattacgat tattttctac
    15481 ctgatatcta tgcacgtcga ctttatcaca atatgcacga tcaaatagaa gaggaagcta
    15541 aagaaaacat ttttattaaa atcgttcaat ctttagacaa ctcaacggca caaaagttac
    15601 ttattcaatt ttatgaagag aagcaaatag atcgtttgca agaatacctt gaaaaattgc
    15661 gtcaaaatga agaaattaca gaggttataa ggaaacgact tttgctagaa gctgaaaagg
    15721 cggtgaaaga atgatcattg gaggaatatt ttccggcatt gccgactggt ggtcaggctt
    15781 atttggggat ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag attatggtaa
    15841 ttatctgcag tatcaagatg tatttagttg gatttggaac acttttctgt ggggattgat
    15901 taaaggatta tataatctca atactttact agaaaaaacc atttatcaaa gctttgattt
    15961 aaaagactta ttgaacgccg ctggggtaaa tgcactattt caaagtttga ttagtaaggt
    16021 attggctctt ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa
    16081 acacccacca aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt
    16141 tggtggtagt tctttaatcg atgaagggtt aaatatcagt aaaagctttt acggagacgt
    16201 cactgcagca aataaagcag ataaaacggg atcacctgct tttcaaatta ttcaaaataa
    16261 tgtgatcgat gtcagtacgt tactagcaac tgacccgaat aaagtagaat ccctaccaac
    16321 agataaaaga aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca atgggattat
    16381 tactcctgaa caagctaaag gtatggcagg taatgataaa agtgggttat cagatataca
    16441 aaaggatcgg ttaaaggcat tacaataccg attggaacta gatgataatg ggaaagaaat
    16501 tcctgttcaa attagtgacg aaggcttagc aaaatatgtc tatacttcag ggtatcgtcg
    16561 atacagtagt aaaccaggta ttatcttagc tggtgaatct tctttggcgg tggcctattt
    16621 atttatccta tttacgatcg ttacttgcat tattgagctg gtatttaaaa aattctattt
    16681 agtgattgcc gcgtcaacgg actttgagac aggccaacgg atgaaaagtg cgatagaaga
    16741 tatttctcaa tccttcttgt tattggcgtt tacgatttta gaattacgaa tctatacatt
    16801 aatgctttca ggtatcggtg atttgcatgc ggcaggaaag atcaatggat tcttgtatgt
    16861 tgttgcttta attgtgctaa ctattgcgct attcaaaggc tctcaatcgg ttacgaagat
    16921 tttcggtgtt gatacgagtc tgaaaaacgg cagtaattct cttcttagca tgtttgcctt
    16981 aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg ctggtaagag
    17041 tggtttaagc catttaaatg atattcgtaa aaatggattt ggccatcgaa aagatgttac
    17101 ccctgataac ggggaagaac aatcagacga taaagctaca actgtaccta atagtaagaa
    17161 aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg cagaaggtct
    17221 tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa gatcttacag aaaaagccaa
    17281 agacacctta gaagagaaat tagacggcac gagagctaaa gaattggctg aagctgtgaa
    17341 gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagaagtca aagaaggact
    17401 aaaagatgcg tatggtgaag gacaagttaa tgcagcagta aaagctaata caacaaccga
    17461 aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa gatcaccaag aagcgttgca
    17521 ctctaatgaa aattcgattg aagagaaaaa ttccatagaa gaaagaaatt cagaagaagg
    17581 aaaaaatgga cagttaccaa aaactgatcc agaacaatat cgctataaac ctaacagtga
    17641 ccttgaaaat gagaagcgaa tttcagattt atcaaaagta aatcaagaag acaaagcaga
    17701 aaatgaggga cagttaccaa aaactgatcc agaacaatat cggtatagat ctgttaactc
    17761 cgaaaatgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    17821 ggattccatc gttctgccaa atgataatgg aatcgaagtt ggaactgaaa aatcaacgaa
    17881 aattggttta cctaaggcac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    17941 ggataatgat caaagttttg ttcatcaatc acctgcaggt actgttcaaa tgaaaccaag
    18001 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttatctg aaagtggtga
    18061 gacagggaat gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa aacctattca
    18121 aaacgttcat acaggttaca agccagcaaa tgtggcattt actgcaccaa caaaaataaa
    18181 tgtgacaaga gaaactaaaa acaggaacaa tgcattgaaa gaacaactat tgagggatat
    18241 caaacaggat atcaaaccat aaaaggccta caagttaggc cttttattta tatgaaaagg
    18301 agaaatcaat tatggataaa tatataaaca tgaaaaaccc tccaagattt agtggagaat
    18361 ggaagttttt cgcttttttc tttttagatc tattaacgct tggtggctgc tttttttggg
    18421 cgaaaatggt caataatctt gtccacttac cactcttttt tgcactcttg aattatggct
    18481 tatggattgc gatagcgtta gtgattgttt ggcgacccta taataaccca ggtcaacgac
    18541 aaatttttgc gattatcgag tctatttttt ttgcggacaa gaaccattat catgcgttag
    18601 atcctaaccg aaattatcat ggtacgtata aggaggatga ataaatgcga gtcaaaggac
    18661 aaaattcagt tgaaaggaaa tccattttgc agtttggaaa accagaagag cgctctatct
    18721 tggacttatt tccgtataaa tcctgtacag aagagggagt cttaattact agagaagatc
    18781 attttcaacg ttattatcgt gtagtttcta cagacgtgga aggattaaat gaacaagaga
    18841 aactagaacg aatgaatcag ttgaccaccg tcatgcgtac gtatgtgcca acaattaagt
    18901 tagttagttt gaccacagag acggatctaa cggaacaaat cgtgcaaaag cggcaattat
    18961 tagataaaaa tcgtatggaa cagtccttag gaagaaatct acaacgatta aaaaaatatg
    19021 aaaagaaatt agtcgaagaa atccaggaat tgaaacgagc agaacaagaa cgtcctgact
    19081 taagcttctt tttcgtcatt gaaggaaaga caatcaagga tttacaaacc agaaaccgtc
    19141 agttaatgcg atctagtggc attttagggt taaaaccact ctacaaaaaa gaattgatca
    19201 agattctgta tcgaatgaac aatatgaatg atgagtagga atggagaaga gagaatgaaa
    19261 tatccgttga tatggttaga aaaagatgtt ccgaaggaag agaccactag aaaatttcct
    19321 cacactttaa gcctgggtaa gttgggaaaa gggcaatctt ttaaccaagt aaagaatcaa
    19381 acaagaagag gtgactgtac atgttagaag caattaaaac agctttttca ccacaaaatg
    19441 atcaaaaagt acttgatcca ttaaagcaac taggaaatcg ggaaatagaa attttaaaat
    19501 ccaaagggta tgatttagat tttttaaaac aagtgatgcc acaaggtggg ttaaattttg
    19561 atgaagaata tgtgacgtat ggtaatggtt attcacgatg tgtgacagtt tatcactaca
    19621 caaaaaatcc tacgcttttt tggttggcca atctcatgaa caatcctcat acagtggcca
    19681 cactcgatgt tagcacagat gaaaagcaaa aagtgattga tcaaatcaat cgtgccttag
    19741 atgaattaaa tgatcgatca gataatgaac gaaaagcgac tgaccgaaat aaaagcgcag
    19801 aagaatacct ggaactagcg gaatatgctc gtaaattgac ttctaatggg gaaatttcta
    19861 agcaagtcaa agtgagagta catgtttacg gaaacacgat tcaagaagta gatgaacgag
    19921 cgcaggaact gatcgaaaat ctaaaaggga tggattatcg tgcggtgact tatctgcata
    19981 aaaccaaacg agattggcaa tcgatgttta ccatgttcga tgaacaagct gaatggttcg
    20041 gaaatatcaa agggcaatca ctaccatcgt taaacattgg cggaggtttc ccgttccacc
    20101 accaaacgct aaaagatcca ggagggacac aatatgggga aaccatgaca ggcggcccat
    20161 ttgtctggaa tgcgacgtat aaaaatagtg tacggttgtc tactaatacc ttgctt
//
LOCUS       DAJSVF010000045        19995 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSVF010000045 DAJSVF010000000
VERSION     DAJSVF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.046"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            3..815
                     /locus_tag="ON550_001665"
     CDS             3..815
                     /locus_tag="ON550_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR3954752.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="ON550_001666"
     CDS             812..1612
                     /locus_tag="ON550_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3954753.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="ON550_001667"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="ON550_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR3954754.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="ON550_001668"
     CDS             3109..4818
                     /locus_tag="ON550_001668"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR3954755.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="ON550_001669"
     CDS             5030..9382
                     /locus_tag="ON550_001669"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR3954756.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="ON550_001670"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="ON550_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR3954757.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="ON550_001671"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="ON550_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR3954758.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="ON550_001672"
     CDS             11220..11513
                     /locus_tag="ON550_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR3954759.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="ON550_001673"
     CDS             11510..11821
                     /locus_tag="ON550_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR3954760.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="ON550_001674"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="ON550_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR3954761.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="ON550_001675"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="ON550_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR3954762.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14622..14843)
                     /locus_tag="ON550_001676"
     CDS             complement(14622..14843)
                     /locus_tag="ON550_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3954763.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15291..17654
                     /locus_tag="ON550_001677"
     CDS             15291..17654
                     /locus_tag="ON550_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCR3954764.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="ON550_001678"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="ON550_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR3954765.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="ON550_001679"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="ON550_001679"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR3954766.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6861 a         3513 c         4389 g         5232 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgca ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaattcaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12661 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13021 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14701 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14761 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14821 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    14881 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    14941 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15001 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15061 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15121 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15181 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15241 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15301 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15361 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa ccagaagatg
    15421 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15481 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatatcgaa gggccaggac
    15541 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15601 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caagcgattt tcatttcctg
    15661 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15721 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15781 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15841 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    15901 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    15961 ttaacggact tggctgggag cctttcttta ttgaaggaaa tgatccagaa aaattaaatc
    16021 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat caaatccatc caaaaagaag
    16081 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16141 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16201 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16261 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16321 aagaaatcag agaaattgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16381 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16441 ttgaaaaact aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16501 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16561 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16621 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16681 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatacgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccactcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgc aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcattaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcgg aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgatca ataaaatgat aaaatcgatc ccgcaaaaca gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatgga cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaaac ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccta tttgtattgg aaaagccaca
    18181 aaagttatcg agtatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aagaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa agatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtgaaacg attcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggcaaagtt gtcagtatct atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtactg
    19021 ggattttttg acggcgtgca tattggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atcgattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgaggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacagttca tttgcctaaa
    19621 gaaggtgttt atgtatcgga aataaaaatt ggggaaactt ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagaatt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcagtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaaaaa ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca atttcaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagcc
//
LOCUS       DAJSVF010000046        19883 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSVF010000046 DAJSVF010000000
VERSION     DAJSVF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.047"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..1425
                     /locus_tag="ON550_001680"
     CDS             1..1425
                     /locus_tag="ON550_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HCR3954767.1"
                     /translation="MRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIVE
                     STYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIPH
                     FSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFTK
                     EMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGER
                     EKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGYK
                     TPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTITR
                     DGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIYK
                     KRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPMA
                     FYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(1447..2253)
                     /locus_tag="ON550_001681"
     CDS             complement(1447..2253)
                     /locus_tag="ON550_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954768.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(2341..3141)
                     /locus_tag="ON550_001682"
     CDS             complement(2341..3141)
                     /locus_tag="ON550_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954769.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(3480..4589)
                     /locus_tag="ON550_001683"
     CDS             complement(3480..4589)
                     /locus_tag="ON550_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3954770.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            complement(4579..5442)
                     /locus_tag="ON550_001684"
     CDS             complement(4579..5442)
                     /locus_tag="ON550_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3954771.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            complement(5511..7346)
                     /locus_tag="ON550_001685"
     CDS             complement(5511..7346)
                     /locus_tag="ON550_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3954772.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(7395..9785)
                     /locus_tag="ON550_001686"
     CDS             complement(7395..9785)
                     /locus_tag="ON550_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HCR3954773.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(10053..11300)
                     /locus_tag="ON550_001687"
     CDS             complement(10053..11300)
                     /locus_tag="ON550_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3954774.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            11693..12109
                     /locus_tag="ON550_001688"
     CDS             11693..12109
                     /locus_tag="ON550_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954775.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(12126..12863)
                     /locus_tag="ON550_001689"
     CDS             complement(12126..12863)
                     /locus_tag="ON550_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954776.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(13285..13848)
                     /locus_tag="ON550_001690"
     CDS             complement(13285..13848)
                     /locus_tag="ON550_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954777.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(13835..14362)
                     /locus_tag="ON550_001691"
     CDS             complement(13835..14362)
                     /locus_tag="ON550_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954778.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(14385..15722)
                     /locus_tag="ON550_001692"
     CDS             complement(14385..15722)
                     /locus_tag="ON550_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR3954779.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            15842..16321
                     /locus_tag="ON550_001693"
     CDS             15842..16321
                     /locus_tag="ON550_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954780.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(16436..16668)
                     /locus_tag="ON550_001694"
                     /pseudo
     CDS             complement(16436..16668)
                     /locus_tag="ON550_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16671..16988)
                     /locus_tag="ON550_001695"
     CDS             complement(16671..16988)
                     /locus_tag="ON550_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954781.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(17099..17281)
                     /locus_tag="ON550_001696"
     CDS             complement(17099..17281)
                     /locus_tag="ON550_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954782.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(17318..18118)
                     /locus_tag="ON550_001697"
     CDS             complement(17318..18118)
                     /locus_tag="ON550_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954783.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(18255..18674)
                     /locus_tag="ON550_001698"
                     /pseudo
     CDS             complement(18255..18674)
                     /locus_tag="ON550_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18670..18978)
                     /locus_tag="ON550_001699"
     CDS             complement(18670..18978)
                     /locus_tag="ON550_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954784.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            complement(19140..19670)
                     /locus_tag="ON550_001700"
     CDS             complement(19140..19670)
                     /locus_tag="ON550_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954785.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
BASE COUNT         5760 a         3983 c         2809 g         7331 t
ORIGIN      
        1 atgagaaaac aatcgattga aggtagaaat caatttgcta tgttaacaat tgatgatctt
       61 gtacctaaag atcacttagt tcgaaaaatt gatgctgcga ttcagtttga tttcatttat
      121 cccattgtag aatctacgta ttcaacctat ggtcgtccca gtattgatcc agtggtctta
      181 attaaattag tatttatcca atatttattt ggtatccgtt cgatgagaca gacaattaaa
      241 gacgttgaaa caaatgttgc ttaccgttgg tttttaggtt attcattcga ggatcctatc
      301 ccacacttct ctacttttgg taaaaactat gttcgaagat ttagagaaac aactttattt
      361 gaagatattt tctctcatat tctagaacaa gctgtaaaag ctggcttcgt tacggaagac
      421 aacctttata ttgattctac tcatatcaaa gcaaatgcca ataaacataa gttcacgaaa
      481 gagatgacct acggtcaagc aaaagcttat caagatgaat tagaagatga aataaataaa
      541 gaacgtattg ctgtaggaaa acgtcctttt acatgggaca cagaaagtga gttgagtctt
      601 caaaaaataa gtcattcaga tccagaaagt ggttactatg ttaaaggtga acgagaaaaa
      661 caatttgctt attctgcaca tacttcctgt gaagataacg gttttatctt atctacatta
      721 atcacaccag gaaatgtgca tgacagccaa gtcgcaatcg atctggttaa acagtcgaaa
      781 agagcttttc caaatataaa gcgtgtggtt gctgatgcag gatataagac gcctaaattt
      841 gttcattttc tgtcacactt gaaactctac ccgattcttc catatactgc acctcatgga
      901 gtgaaaggaa tgtttcgtaa aaaagacttt gtttatgatg cctattttga ctgctattta
      961 tgtcccaata atcaaccgtt gttattttca actatcactc gtgatggtta tcgaacctat
     1021 aaatcaaatc caaaattctg tgtgagttgt ccattacttg aaaattgtac tttgagtaca
     1081 aaacatcaaa aaatagtgac tcgacatatt tgggctgatc ttatggatga acttgaacac
     1141 caacgccata cggcgttgaa tagagaaatt tacaaaaaga gaaaacaaac cattgaacga
     1201 atatttgcag atgcaaaaga aaagcatggt atgcgttgga cgaaatatcg aggtcttgaa
     1261 aaagtcgcca cgcacacgat gcttacgttt gctgccatga atttaaaaaa actagccaca
     1321 tggctatgga aaacaaaaaa gcctatggct ttttatttca aaattatgca aagactaaca
     1381 aaaaggagtg gcttaattac caactccttt ttgtctacaa tctgaataga gcttttcagc
     1441 tctattttat tcaatatcat tttgatcaat taataatcgt acactccaat taaccgagtt
     1501 ttgatcactc aaatatttat aatctttgtc atcaaaaacc agttgccaaa ctctggtacg
     1561 aacagtttct tcgtccactt cctcatctga agagtattgc gataatgctt cttcaactat
     1621 atactttaca acttcctcaa aatcagtagg aactttatca ataaccactt catagtcttc
     1681 ttgttttacg atattgggat caatatgttc atccaaacat acctcagtga gtttttcttt
     1741 cacttctgta tctctttctg tttttaggta ctctgcatag tcttccttag accattgttt
     1801 ttcaagatca tccgctatac ttttaataaa ggattcttta tttaaccaat tatctgcggt
     1861 atctatacct tcaaactcaa aagctgttcc ttctatacgt gatttgacga aagcgtcaat
     1921 tttcttgtat tgattattca tttcccatga aacacactca tcttcttgcg taatatcata
     1981 aattttttcg gaaaaactaa ctaattcatt ccacgattta tcctcggcaa cttgacttag
     2041 tttcgatgca ataaaaggaa attttgcttt taagtcatcc atattcttga tctcaaattc
     2101 gatattttct tctcctacag ctgaaatcca tgctgaaaga ttgattgcaa tatcgctaaa
     2161 gtcttctaca ttttcgacat ttcctgtatt atcttttatt actgcttcta aagccatatc
     2221 ggttgttgca ctcatggttt gatctcctct cataatagag caaaaagccg gtttaaccga
     2281 ctttttgcat ggtacgtttt gtttggtagt tttctgcttg tgtttatgtt gaaataatcc
     2341 ctatagctct tcttttaata gttcttcaac tttatctctt aactctccct gaccactctc
     2401 taatagattt aaatagctca tcagaatatc cgtttcatac gtttttaatt cagctctata
     2461 ctcactttct gaatatgatc gtaaataccc atcattgcta aatgcaacta gatcatcttg
     2521 aaagttccat tctcctaaat ttgttttact tactgcttcc catggactat tactaaacat
     2581 ttcattaaag aaatcttcgt cattttgata taccttcaat tcaggaaaat agttgctgtc
     2641 ttcattgatt tcttgcacca tttttccaag ttcttcttca gataatttac cttccaataa
     2701 ttttactttt tctctgtcat ttgcataagc catttgaacc tctccttttt ctttttcaat
     2761 ttcttcaggt gtttgttttg gcgtaacttt caaatctatt ccattgccta ttagacctat
     2821 tagataacca tagaacgttt taaagtcact tgtagcaaga gatttggcat cagggtatgt
     2881 atcccctcca attttatcta aaacaaaatc agcaatcgtt acccaaggag aatatatcgt
     2941 acatactaca tcccatgtag catcttcaat attcgtccat gtcggaaagt cattttcatt
     3001 caaatagtct tttttcattg tttcgatcag agtttttcca ctttcgtttt ggcttagata
     3061 ttcaacaaat tctgcaccat caaacctgct actaaatagt ttagctaatg agtctaaagc
     3121 tgtttttgat actcccacca ttactacacc tcctattaat attttacgat gacttgtttt
     3181 tcattcacaa ataagatttt ctacatctta tctattgctt tatagctttt ccatacatta
     3241 tttctaaata caaccaataa ccctattaaa ttaagctttt gagtttatct tttctaataa
     3301 tattctgata aatattctga taaaaaaatt tattgtattt ttttcttgac tttttcttta
     3361 gaattaagat cagttttgat tttatttaaa agtgaatcta gctctattca aaataaaaaa
     3421 gagatcaata atagtatcaa tcccttttta ctgtactcta tcaattttta taatcttcat
     3481 tatgcttttc gaaactcttt gattaaaaga ataagaacct ttagacttgc taatgctcct
     3541 attacagtcc aaatgactaa aagcattttc tgatgtttat tggtaattcc tgtaaatatt
     3601 gaacttgcct tttcagcaaa cgttctgtct tgcatctcta cgctaccttg ttccggaata
     3661 ttaagatcat tatttccatc taattccttg ttggcacgat caacatcttc aaataaatca
     3721 ttagaagatg taacagtttc tgtcttatct gaaacggact cgctatcaat ggcatcagcg
     3781 tatgcggtat tgccaactgt tacttgtggc gtgatgtatt ttgctttttc atctcttaga
     3841 cttctaacta ataaacgatc gtagttctga ggatatggat gacaagtata aagtgttaac
     3901 atatctaaat caggaatatc agtcagtgtt gaattctgac cgtaatttct aataacgtct
     3961 gttcggaaaa ccgtataaac tgctttttca ccaagataat ctatataaat gtggtctccg
     4021 gctactaatt gatcaacata aagaaattgt ggtgacgtat aaccgccacg atgacctgct
     4081 attactgttt gatgaccttt acctgataaa ggtagactag tagcactcat ttgtgccaca
     4141 cctctatata aatgctcctg tgaagcgcct agaaacaaaa gctctagcac atttattttt
     4201 ggaatataga cataaccaac cgctcctgaa taggtagatg gttcaaaatc tttcgtttga
     4261 ttttttgaaa aaacatctcc tgtgtatgga ttgacctttc cttcagcttc acgaatatca
     4321 aatgatccac cgtctgtttc ctgagtattg tctactcctt tatctgtttt ctgagtattg
     4381 tctaatcctt ggttaccgct gttcgatttt atttgttcga ctttttttgc cacttcctct
     4441 tttgaaggag tttgtttttt ctcaattttc tcttggattt ccgccacatt ctcttttgtt
     4501 ttgaattgat gatagagctg atttgcagga taaattgtta aaaccaaagt gcaaataaga
     4561 gtcaaaaaaa gtagtatttt attcttcatt tttcattcct cgtctctttg ctactatttt
     4621 tttaataatg actatcacag ctattattaa taaaacccct acaactacga taggatacca
     4681 gttcatctgc gcctttacct ttaaatcttc tttcttcttt tctttctcaa caatactttt
     4741 ttggtaaggt atgctaaccc ctctaacgag taacctatga gaattatctc caacaggtgt
     4801 acatgtcata agtgttacat aatcttttcc tgccacagga agcaaatcct caaattcggt
     4861 tggtagaatt gtttttatcc gatctacttg ataagcaagt tttttccctg aaatatcaat
     4921 aaaaaattta tcaccaagtt tgagcaactc taaatctgta aaatattttt tattaggaat
     4981 gcctgaatga gctgatataa cagaatggtt tccaatccca ccagttggat aatcagttcc
     5041 ggttaatatt cctgctcctg ctgatagttg gaaatcgttt acaccatcat aaattggcaa
     5101 atccacatta atctttggta taagaatttc acccactaat tcacctaatt tatccttctg
     5161 aagatttcca tttgacggaa tattgttatt atcttcagga atcgtgactg aggtttgttc
     5221 tgtgtctttt cgtttcctat taacttctga atatctcttg aagacttctt cattctcctt
     5281 ttcagaagct tcatcatatt tttccttata ggagtttagt gtttgttgag tttggtagta
     5341 ggtataagcc tgagtaatta caggatatag acctactcct aaacatagca acatcccaat
     5401 ttttaagatg atcaacttga tattcttctt tttcatgctc atacctactt ttcacacact
     5461 ttttaaaaag aaaccctaga gccaaaaaca ctctagggaa ttttatttca ttaagctttg
     5521 tgttgttctc gtttgtagac aacatatagc cctgttcctg ccacacctgc aaccactaga
     5581 aatactactg ttccaattcc acctgtacga actgtttcag gtgttttaag gttcttgatg
     5641 tctgttactt cgctagaagc atcatcattc gataccacaa atggaacatc atttctagtt
     5701 ggtgtatatc ctgaaggagc aacaacttct tcaagagcat aatcattgat ttttttatct
     5761 gtgattgttg gattaccttg tttatcaaaa tctacatcat aggtatattg caagccttca
     5821 atttggaact taccatctga tccggaaacg aattcagtag ctttagattt atctgcaatc
     5881 caagtaacag atttcgttgt tggatcttgt tttaggtaag taactttatt cggatcaatt
     5941 ttgttcttca ctacaaattt tgctccactc aatgctttgc ttgtatctcc atctgtttta
     6001 atgaattggt gatcaccaat gattgtataa gcgtgatcaa ctggtgtttc gtcttcgggt
     6061 tcatatccat tgttgtagat cgttgaaaca tcattcttaa gtggtgtatc cattttagct
     6121 ttactattag tatgagcgtt catcaaaatt gtaaagaagc gatcccctgc atcaaattgt
     6181 ttctgaagct tatccatacc tgattcagta aagacatagt ttgtcgtttg tccgtcacgt
     6241 tcgatactgt aatcagtatc taaagtcatt ccttcgatca cttctgatga atcataagtt
     6301 aactctgcac ctagaacatc tttgacaaca tatttttcgt aattaccaat attaccagga
     6361 attgatggtt tcaatgtata ttgaacatct tcgccagtat caacggtttc tttatcaatt
     6421 accttagtaa ttacaggtcg tttgtagttg gtaacatcaa gaactacttt agcagtctcc
     6481 ccacgtttaa cgctaaattt gaatttttta ccatctagta aataggttgt tttattagca
     6541 tctgttccca aatcggtttc taggaaataa tagtcaccta attccaaacc atctacagtt
     6601 aatttacctg tgctgtctgt tttataagtt ccaacaacct gatcagatcc atcatctttt
     6661 cgcaattcaa aaacagttcc ggcaagagcc tttccatctt cgtctttttt atcaagctct
     6721 gctgagttta attggaaata gtttttagga tagacatgta catcataatt aaagtattga
     6781 tcatctgatc cgtttagttc aggtacgtta acaatcatag gatacgcttc ttcaactaca
     6841 cctttgactg ttcctgtttc aacaatcaaa tacaagttat ctgttaaatc gtctgtattt
     6901 acttgtatag gtatgtctat attcccttgt gaatcagtct tcattctact tacttcactg
     6961 tattttttgt ttagtaataa tcgtgcttta gcatcatcaa tatgttgacc ttttgtagta
     7021 tcactatccg tccatcccaa ggatttagct tcatctttag taagtttcac ataagagaac
     7081 tcaacgcctt caagaggttt tacaccattt ggtaaagcta tttctttccc tttgttatca
     7141 atatctgaga cagggttccc atccccatta gtatttgtta ctttatggat atgaatcgtt
     7201 ccagttcctt gataattata gttttgttgt gcatatgcta cttgcccccc tagtccaagg
     7261 ctagaagaga ttgagcttgc gcccaatccc actcctagca aagtaccaaa tagcattaaa
     7321 tttgaaaaaa ttttttttga tttcatacgt cagccttctt ttttcatatt tacttctttt
     7381 ccattaacac ttttttattt tgctttgtgc tgttctcgat aatacagtag caccatactc
     7441 aatcctgcaa ttagaaatac aagaaggaag gctgatgtac cgattccgcc tgttcttact
     7501 gtttcaggta caaggtgatt aattactgta tattcaaagg tttgtccatc gtcagcaacg
     7561 gtgatgcctt ctttagacca tttccctgta taaccattag ggttttgtgt ttcttcgatt
     7621 ttgtacgttt tgttggcaac aatattaaat gttgccaagc cattgctgtc cgttttttgt
     7681 acaactttta cacctttgtc gtctgtcaaa gtgaattctg cgtttgctaa tgctttccct
     7741 tgatcatcca ctttatgaac tttgatcaca ccttttttca aagtgttttt agcagtatat
     7801 tcaaagactt gtccatcatc tttgacggta attccttctt gtttgaatgc gcctgtgtaa
     7861 cctgttggta cttttgtttc ttctaagtta taagttttgt tcgcaataat gttgaacgta
     7921 gccagaccag ttgcatccgt ctttttaact tcttttacac ctttttcatc cgttaaggtg
     7981 aattctgcat ctgctaaagg ttttccatct tgatcgactt tatgaacttt tactacacct
     8041 tttttcaaat tatttgtagc tgtatattca aaagtttgcc catcgtcttt aacggtgata
     8101 ccctcttgct taaatgcacc tgtgtaacct actggtactc cggtctcttc taggctatac
     8161 gtcttgtttg ctacaatatt gaagcttgca agtccatttt catctgtttt ttctacttgt
     8221 gttgcacctt tttcatcggt taaagtgaac tctgcatttg ccaaaggttt tccatcttga
     8281 tcgactttat gaacttttac taaacctttt ttcaaagtgt ttgtagctgt atattcaaaa
     8341 gtttgtccat catctttaac ggtaattcct tcttgcttga atacgcctgt ataaccagtt
     8401 ggtactccgg tctcttcaag gctgtaggtc ttgtttgcta caatatcgaa gcttgcaagt
     8461 ccgttttcat ctgttttttc tgcttgtgtt tgggatgaat catccgtcaa ggtaaattca
     8521 gcattagcta atggttttcc atcttggtca acctttttaa cttttacttt acctttttta
     8581 attgtgttcg tagcagtgta actaaattct tgtccatcgg tattaacagt aataccatcc
     8641 tttttaaatg aaccttgata accttcagga acttttgttt cttctaagct gtatgttaag
     8701 ccttgttgca aatcaaacgt agctaaccca tcatctccgg ttgtcttaac agatttagca
     8761 ttgttattgt cagttagagt aaactccgct ccttttaaag catttccatc ttgatcaact
     8821 ttcttaactg ttacctgacc aacagctctt gtccatttag cactcaaaga tttagagtcc
     8881 tttgtttctg aagtagtgat cataactacg gcatcttgat acacacctcg tccatcaaaa
     8941 gtaaaaggat tggttgtgct aacattagca gtacctttaa atgacaaagt accacttggt
     9001 gtagtcacag gtactttaat agtaaaactt gaatttaatt tcacagtgtt gttagtaatt
     9061 tctttaccat ctgcatcata aatcttcata ccagctggtt ttgctgatgt aaaagtgagc
     9121 ttcgcattac cagtcttatt tgatgctgtt ttatatgtga aagtgtgata ttttccttcg
     9181 tcagctgtct tagaagctgt gatagaaaac tcagtgttgg gcgtatcttt tccattctta
     9241 gcatagtcat aaattttttc aaatgctttt tttacacgag ctgtttctgc ggcggtatgg
     9301 tttggatgtg accatacaat ctgtgaaact ttaaagtttc cggctaaaac ccatgaaact
     9361 aattgtgttg cataccatgc ttctgtatca tttcctgcac ccagttcctt tgctgttttt
     9421 gatggaaaac cacctttaaa catacgatag aactcatcac tgccagctcc cttgtacttc
     9481 aagcttccgg ctccacttcc tgttggggat ggcaattctg aatccataca atatccaata
     9541 tttccatgag aatctttaaa ttgacttgaa atcatccatt catttggatg attcggcata
     9601 ttaaccctaa tataaccgtc tgtttttacg ccagtttcta taaattttgc acttgtcggt
     9661 tttgtgacat ctgcttcagc tcttattgct gaaaaaggca ttataagcgt agataacatc
     9721 attactaaag tcgcaaaaca ataaattatt tttgatttta aaattatcgt tttttcatgc
     9781 ttcatctctc gttactcctt ctttatacca gttaggcgaa gaaaatgagc cacgatatgt
     9841 cggtgtggta atgaccttcg cgcccattcc taagttaaaa tcaccacacc tattaatcat
     9901 ttttttcgtt gaaagcaagc tgtttcaacg ctctagtatt catagcatca ccccttttag
     9961 ttctacccac catgagcaac aagttttcta agtagttaag caaggacaaa taaagactat
    10021 ataactactt agtatataaa cgatatcttt tattaacgta ccgaaaccgc taattgagat
    10081 acgttatatg ctgtttcaaa tgatcctgca ttaatccaac ttgccccaaa cacaggatcg
    10141 acaacatggt aaaaaccgtc tttaaatcca tcaacggcta ctacatgtcg attccaaata
    10201 gcatcgcccc aaaacattgg tttatgttga gcaggttcaa aggcataagt tacccacgct
    10261 actacaggat ttccatttac aatctcatct tttaatcctt gtaccgatga accagaaata
    10321 tctacaacat ttccgccatt agcaacaccc catgaaacta caggagccgg attcatacct
    10381 tgataagttc catccacatt gtgtctccat tcaccagaaa aaccattatt tggattattg
    10441 tcaggagata gtggcattga tttaacaaaa gtcattaaat cttgatttgt tgcaacacct
    10501 ttataatgta acgcttctag caaagatgtt ccttcgcaaa gcattgtgtt accttgacta
    10561 ataaatggaa cgcctagcac aacattagtt ggttgttcgg gtttacttgg tgaagtagaa
    10621 ctgtcagttt catcactcga acctgtttgt gattgatcac tagaagatgt actggttgat
    10681 tcatttgcat catttgatgt tgattgtgag ttatttgttg atgttccagt atcagcttcc
    10741 gtagtgctac tttcacttgt tccctgtgat gcttctttct cacttgtaga attatttgta
    10801 ttagatgaac ttgctccatc aacacttgaa gcacttgttt ctgtcttatc agaagtgttt
    10861 gctgttggtt ccgattcttt tgtagtctcg gattttttgt tctcttgctc cgtattttca
    10921 ataggatcag gtttatcttt ttgaacagtt tgactaacta cttcgccatc tgcttcctta
    10981 actgttaatg tatctttact gatgttgaaa gttcgtccaa tcgggatagt ccgttgttct
    11041 ccttttgcca aatcaacatt gttcaattcg gcaaactcag caacagtagt cttgtaacct
    11101 aagcttttaa gcttctctac agttgaccaa atagtatctc cttttttgaa cgcataactt
    11161 gattgtcctg ttgtaatttg gatttgatcc ggtgtgtttg cagtccattc atcagcatgt
    11221 gaggttactg gtactgataa ccccgcaagt acagctgatg ccaataaaac cataccaagc
    11281 gaatttctct tcatattcat tccttctttt tgtttttatt ttctgtttgt gatcgcaatg
    11341 cttctattcc ttcttccgtt ataatatagg ttttgtcacc cactttcttg aacgaacctt
    11401 taggcaaacg atttggataa tagttgatta tgtttgtaac ataatttcta cctttaccca
    11461 aaatcaaact tgcttccctt gcggtataca agttgtattt gtctaggttc aatttctttt
    11521 tgtttgcgat ttagacctac ccctttcgca aaatttattg tataataata gtaccattgc
    11581 actgtcataa ttgcaatatt tttttggggt ttatcttcat ttaatagcct aaaaaggctt
    11641 gttagataag gaatatctaa ctaatcaaaa caaaaaagga tgttaagaaa aaatgaacaa
    11701 aaaaaggaaa ttatcagtat taattacact tgtttctata ttgactattc ttagcgttat
    11761 cttgtcgctg aaaatattca atcaaataaa cacaagctca aatgccaaag atggaatagt
    11821 cgtagaaaat acctctaatt ctaaagaaac tggtactact acaaaaaaag acaaatctgc
    11881 tgagatcact acgaaaaatt atttaataaa gactgtttat tttgatgaac gaaaaacagc
    11941 aattctcaaa gatgaggaag gatcagaatt aaagctacct ataacggtat ccgataagtt
    12001 caatggaacg ttctccttac aaaccgctga aggatcacct gacaatctca aagttgggca
    12061 aacaatcatt cttggtaaaa acgacgaact gtattcattt gtaaaataaa aaaatggagc
    12121 aaaggctagt caagcccttg ctccattttc cattcttcaa gaggtgtagc tatttcaaca
    12181 ccaaagttta aatactgtgg atgctgtaga tacatgatat ccattctatt ttcgataaac
    12241 tcttttaacg ttgagtagat gtatttttct aatgctatat ctgcccaact accataatca
    12301 agtttattcg tgatcttgac gtttatttca atttgagaaa catcaatatt ttgaacatca
    12361 ggaaacttat tcagtcttag ctcttctcta accgcttcta ctagcggttc atattgtccg
    12421 cctagttcgc taattgttga aactatttca tctgaattcc aacgatcttc caataccttt
    12481 gctacatttt ctttaggcgg ttgtctttct ataaattctt caggtgtttc atcaaactct
    12541 actctaaagt tcaatttatt ttgattgtta gcatacatat aatcatctct gtttgattca
    12601 aaaaagttac gaattcctgt gtctgattta tcacttaact ccttatttgc taaagtagcc
    12661 caaaaatccc aagttccttt attttcagta tacaaagtaa aattaatgtt tatatagttt
    12721 gcagtttcag gatacccttg ctctctaaga gcatctttaa caatcactgc taagtcagtt
    12781 tcaaaattct ccataaaaaa ttcactaaat tcttttggat tccacgtttc ttctaataac
    12841 ttttctaatt tatgatctgc cattattatt ctcccccact ttactttcaa tcttatatct
    12901 aaaactgctt aaaattaaga gctatacctt atcataaaag gtattttatg ctttatttaa
    12961 gtctacaatt ctatattaca attatcatct agaccatttt cttatgtcaa ataaaccata
    13021 tatattgtct ttaatagttt tatttgactt tttaagaaag tatttacttt tgactttttt
    13081 tacgttttac aacatatacg tattgtaatg ttcagctcaa tttattataa tctaaatgaa
    13141 ttgattaata aaaattctga ggttcgggtt ttttatcaat tcttttctgt ttgtgtttgt
    13201 gatgaaacaa tcctttttcg gtaggttttg ttatcatcgt cagcacaaag agctaacgtt
    13261 ggtaccgtta gctctttttt tattttactc tgctcttaca tagctatcta aatcctccaa
    13321 ataaaacaat tgattttctc ctatgcttgt tactccacgc caaattgttg ttgttgctgt
    13381 tgttggagta tttcttgtct gtgtaatcaa ttcaaccatt ttaccttttt gatttgttgt
    13441 atagtaatca gcagtatagc tagatattgg actattacca gtgcctacca tcgtgccaga
    13501 aatatcaaca ggactagact ttactatctg aggaaattct ttttgtattt ccttcatatt
    13561 cttcgtatat ttttcccatg aattccagtt gtaaaataaa ttaaagaatt tcctactttg
    13621 ttccttaatt tcagcatttc ccaattcttt gtattgagct tcctgcatag ctttcacttt
    13681 ctggttattc ttgactactt cgatttgttt acttttaagg tcttccaatt ctttgacatt
    13741 attatcataa gcatttttct tttgaaatag taccacacaa caaaggatca caattatgga
    13801 aagtattccg atcgcctttt tagcagtagt gatttcatga ttttgcataa aaagcacccc
    13861 cagtcacttt cttatcatta tttagtgtga tggttaatgt ggtatcatac actttttttc
    13921 cattgtattg aacttcaaaa tcggtcaatg cttcatacaa cccactacta gatgtcttaa
    13981 ttccaaagtt tgttagctta aaagtaaaag gcttatagta ctcgattttt tctttctcac
    14041 tactcttatc agtaattggt ggtttacctg ttacaataaa tagttttctc aaaaattctt
    14101 cacctgattg atctgcaaac ttattttgaa ctgatccatt caacatttct tgaatatcat
    14161 tttctaaatc tttccgtaat ccatccacct catttggttt aacctcttta aagaatgtca
    14221 gcttatcttc aatatgcaca ctgtgttttt cagcttctat gtcctgattc tgcttattga
    14281 agaataggat tccaacaagc agtgctaata acataccaga aagcaaagaa atgataattg
    14341 tatctttcac tttactattc atctatatcg tccttcattc caatttattt ttcgatttca
    14401 aatttagctt ttcctacacc gctaaagaaa tgcacattat ctgatccgtc cactccatca
    14461 atcaacgtta ccgaaactcc ccttgttgga gcttgggttg gtggaacatt aaagttagca
    14521 catagaaatg atccatcttc aaacaccgct ataactacac cagtatgccc tgcatctcca
    14581 acagcccata accatccatc cgaagcacta aacccataac ctacgattgg tttgttcgtt
    14641 atttttgctc ctgccgattt gtagctttcc catacataac caccatttcc agtagtcggt
    14701 tgattcccat cgtataattg gctcatataa gcccacgtca actctgtaca ctgaccttga
    14761 tatattccgt taaaaggtga agttgaccaa tctggttctt tgaattttgg tagcgtgatc
    14821 gctttcccat attttttcaa agcttcttca taagtcatat tcgctattac ttgacctttt
    14881 ttaccgttaa tttttccttc tttcccaatt gatccagaac cagaattagc gtcatccgta
    14941 gttgcacagg catcttctac tgttcctgat gctgaacttg atgaattatt cgatccatct
    15001 ccggtacttg aacctccgga tattttattg atcttacttt catcttttgc accgtccata
    15061 cccttgcttt tcatgtactt ccagatttct tttgcatttt caccacgtac agctattaca
    15121 gctgattcgg aatcagctga aatttcccag tttttatgaa aagttactgc attttctacg
    15181 acatcatcag aagcatttaa agcataatct tttaatattc ctacgaatcc cccatcacct
    15241 ttgaacataa agtccaattg ggcttctgta gtccaccaag aaccgccatt atttttctta
    15301 gaccaatcaa ctaatgctgt atgtcgccca gctgtccatt gaccatagcc aatacccatc
    15361 agaccatcag taaattgttt gatgaagtca acttctctct gtccatctcc gtaataccct
    15421 tctactgctc gaggatcaat acctgattct tgtttccagt tacctaaaat acctgcaata
    15481 ctagcacctg ataagccata ttctccattc atgtatttgt aaatatcttc aatatttttg
    15541 gctacatcac ctgttacagc gccacttcca gtatcgtttc catcgctatc tgttcctgac
    15601 ccactacctt cagtagtaca gttaaattgt gcatcataag tagctatccc atcagcatgt
    15661 atcacttgcg gatttattgt gattacactc attgcaatcc ctgtaaattg caccattaac
    15721 ataagcaatg tggcaatctt tctcttagat tttttcaata actacgcccc caaaaagtgt
    15781 acttttttat gttattatga taatataata acataaatta atagaaagga aaagctaata
    15841 aatgactgat aaaacaagtg atttacctaa actagctcca aaaacatctc gagtaaagag
    15901 caaaaaagat ggaaaaacaa aaaatgctgt aaagaaaaag tcagtaaaag aaacatcctc
    15961 gttactactt gatattccaa tgagttcttt tgatgacaat acggacgaaa cttctgttaa
    16021 aattgcaact cacgttggaa tgaacaatca aaatgattct ttttacaact tgttaaacag
    16081 cctaagagag ttggaagata aaaaaaatca ggtaatcgca tcaaaagctc ctgatctcac
    16141 aaccgatttg gatgctgaaa ttatccaaat caaacaagat gagcgatcag ttaaatcgct
    16201 actgaaaacc tactttgatg gttttgataa agaactagct ccttatagcc aagcaatcct
    16261 aactaaacca atacaagctc cacatatttc aatttcccta caaaaaaaga gtgatgatta
    16321 aacaatcatt actctttttt agatttctgg taagcgctga tcgcaaaaac catcaacaat
    16381 attgattcaa aaaatatgtt cattcgtgtc tcttttttcc attatagatt tcccactatt
    16441 ccattaaatc gaaagtcaca ccgattacta caaagattaa cgtgaccaca ttcaaaaaca
    16501 ttaaaagata tgctgataat ctcatcattt ttttagttca cttactggta atctaaacat
    16561 ttgaacgatt gcactggaaa taccataatt agcaatgaca aacaaaaaat acgcaataat
    16621 ttcacttaca cctagataga tcagtaaaat aaataagtca cttaacattg ttagtttcct
    16681 cccagtatat gattttcatt tttgggaagt cttttataat ccaattcctc aaaaaaagga
    16741 accatagtgt cactaatata ggttttagtt tcaggtaccg ccaaaattcc ccaatcaaga
    16801 atttcttccc atgatacatc atcaaattct aaaatattat gatcaactga cctattacca
    16861 agttcatatc caccaaagat caccatatta tcaatcatgt atagaatttc atcaggcgaa
    16921 ccatgagtta atgtttcgtc ctcttcaagc agttcgttta atctttgttt gtattcctct
    16981 cttttcatac ttttatccct tctgactcat ttttttgaat tttaacattt ttaacccaag
    17041 catgactatc tttacatgct attaattttt atctccattt tgatatttct gtttttttct
    17101 actcaccaat caaccgtaaa tcctcaatat tctcaccttc aagcgaatat gaatatactt
    17161 cgccgtaaat attttctata actacgactt cttcttcaaa gtctatggac tgcacatcaa
    17221 accattctcc tccgccaaat tcgtctatga ttagtacctg caatatttta tttactgcca
    17281 tttcaatttc cccttatacc attctatgtc ttccaaacta tttttcttct ttccatacca
    17341 aggtttcacg tatctcaaaa ttttctttaa ataagtgttc cggtactttt ataattaact
    17401 cttttcctac ttttatcaga tagtcttttt cacctaatga gatttgacca gtcggtaact
    17461 gtatagaaac tcttttagtt ttattaaatt ttttagtttt caatacagta tacggtctac
    17521 agaatttctc taagcgttca aatcttttac tatcgtattg gaaataagct ttaggaacag
    17581 ataaagtaca aaaactatcg gtaacagcac ttatgtcttt tttagaaatt atcccaactt
    17641 tagaacttct aaggaaagtt gctaatttct tatatccacc atgacttgtt tcaatataga
    17701 tgctattgta ttggctgtcg tctatctcat ttattgtttc aacctttttc ctgacaggtg
    17761 tttcaacgag ttctcgaacc tcttcttcac tgtacgctaa tacaattaac tcttccccat
    17821 ttaccatctt tagaacatac gcttgcttct tttttcttgg cttattttga gattcaacat
    17881 aaccagacat ggaatcttta tacttccaag catgtacttt tttctctaat ttctgtatct
    17941 cttttttcag ctgtagatat tcagacattt tttcattgta acgttcgcaa tataaacaat
    18001 attcatcttt tccaaaatca tgatcaagtc cttcttcaat atgtttattc aacaaaccgg
    18061 aaaaatactg atcatactgc ttctttcgta acttcagctc tcgtaaatct tcagacattt
    18121 catttttcct ttccaattat tggtgccatt tcacaatttg ggattgacta aacaccataa
    18181 ttaatttgta agctaacaca attacaactg ttggtaattg tgttagtctc catcaacttg
    18241 tctggtggct ttttttattg cacaaatttc atagctctca aaaagcgctt ggcttgaaaa
    18301 ggtgttattc tacgaacctt attcgccaat ttccaatctg ataaagcaat ttgccattcg
    18361 tgcatttcaa aatccggcat gatttctttg atatactgta gctctttctt ttctctttct
    18421 tttaagaaac ggctcatcga gtttaacatt ggaactaagg atgcgtaggt acttgaataa
    18481 cttccaatgc gtttcgtagt aattacataa tcaataaaaa cactattaag aaaggtcaag
    18541 tttataaact ctgattcata cactctaaaa tttgcagtta atcgcttcgc cttgtcaccc
    18601 caatagtcac ttttgttgta ggctttttcc cctcgaatat agcactctgt ttctctttcg
    18661 taataatagt catacttttt tcatcatcaa atctgagagt ttttgattat ctttttcact
    18721 gtaaaataat ctactcgtta aacttttagg ataatctttg atagaatatg catcccaaac
    18781 aattctttga acatattcac gatctatatt ttctctgact tcctcactcc aataatgttg
    18841 accgtaatag catttttcta ttacgttgtt agcagaaact tcaaaatagg attgttccct
    18901 ttgtatcctc ataccaatca tcatagcgca ggaaataact attaataaca atcctgtttt
    18961 taatctatta ttccacatgc agtttccaca tccttatctt gataaaaatc ctcttgaccc
    19021 tctccaatta ctgttttaaa tataaccacc aaaacaagat catgctaaga aatccaagag
    19081 tatataagat gatacattct ttgttaatta tccaaaaaac tctgatttct ttgagctttt
    19141 cacgcattag gaaacacctt gttatatctt ttttgtattt ctttcaattg ttttttaaaa
    19201 ctcataaaca actgttcatg atctggtcta agcgttagaa tatctaaatt atttatcttt
    19261 tttaattgtg taataaaatc tattttggat tttctttcaa tatcaaaacc gacaataatc
    19321 tttcctgttg gatcaacgca tttcacttga ataactttgt cattttgcga tagctctaat
    19381 attgatgatt tttcttttgc ctcttccaaa agatgttcta acacttcgtt aggtgttcca
    19441 tgatagtaat aaatcatggt cttctcgaaa atttttatcg gatcaacacc atattctttt
    19501 ccgattcgat aaaaaaattt cattggaatt tcttccggat ttctgttcct gcttgaccag
    19561 ttagagatgg tttgataatt tgcaaaatgg attcgttccg ctaatctctg aactgtggtc
    19621 tctaaatctt cttctattaa ttgttttaca gcactaatgt tttgtgacat ttgctttcta
    19681 tcccttcaaa ttcgttgcat tatcaattct aagatttcat gttttagtgc atttctatct
    19741 acattttgct tttttaactc tgcaccataa tatcttcgca atcccttatc caagcataca
    19801 attccttgat ctaatagatc atattccgct ttttcaaacc ttcgctttgc ctgctcaaca
    19861 agcaaaatga tattgtgatg tat
//
LOCUS       DAJSVF010000047        19773 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSVF010000047 DAJSVF010000000
VERSION     DAJSVF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.048"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            6..305
                     /locus_tag="ON550_001701"
     CDS             6..305
                     /locus_tag="ON550_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3954786.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ON550_001702"
     CDS             378..1739
                     /locus_tag="ON550_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3954787.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ON550_001703"
     CDS             1761..2021
                     /locus_tag="ON550_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954788.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ON550_001704"
     CDS             2035..2787
                     /locus_tag="ON550_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR3954789.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ON550_001705"
     CDS             complement(2881..3486)
                     /locus_tag="ON550_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3954790.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ON550_001706"
     CDS             complement(3539..4513)
                     /locus_tag="ON550_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR3954791.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4892..5611)
                     /locus_tag="ON550_001707"
     CDS             complement(4892..5611)
                     /locus_tag="ON550_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3954792.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5738..7009)
                     /gene="celB"
                     /locus_tag="ON550_001708"
     CDS             complement(5738..7009)
                     /gene="celB"
                     /locus_tag="ON550_001708"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3954793.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7162..8559
                     /locus_tag="ON550_001709"
     CDS             7162..8559
                     /locus_tag="ON550_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3954794.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8640..9080
                     /locus_tag="ON550_001710"
     CDS             8640..9080
                     /locus_tag="ON550_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3954795.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9228..9554
                     /locus_tag="ON550_001711"
     CDS             9228..9554
                     /locus_tag="ON550_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3954796.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9555..10304
                     /locus_tag="ON550_001712"
     CDS             9555..10304
                     /locus_tag="ON550_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954797.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10384..10839)
                     /locus_tag="ON550_001713"
     CDS             complement(10384..10839)
                     /locus_tag="ON550_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3954798.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11266..11541)
                     /locus_tag="ON550_001714"
     CDS             complement(11266..11541)
                     /locus_tag="ON550_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954799.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11835..12650
                     /gene="thiD"
                     /locus_tag="ON550_001715"
     CDS             11835..12650
                     /gene="thiD"
                     /locus_tag="ON550_001715"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR3954800.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12979..14430)
                     /gene="cls"
                     /locus_tag="ON550_001716"
     CDS             complement(12979..14430)
                     /gene="cls"
                     /locus_tag="ON550_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3954801.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14771..17437
                     /locus_tag="ON550_001717"
     CDS             14771..17437
                     /locus_tag="ON550_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3954802.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17801..18553
                     /locus_tag="ON550_001718"
     CDS             17801..18553
                     /locus_tag="ON550_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3954803.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18575..19729)
                     /locus_tag="ON550_001719"
     CDS             complement(18575..19729)
                     /locus_tag="ON550_001719"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR3954804.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6303 a         3473 c         4054 g         5943 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaaa ctagctttaa aaagctaact ttagaatcat tacgggtggt
     4741 cataagtctt tttatgctga gttatttttt catttttgag ctaatgctta ggatagaata
     4801 gaatgttgat ttcacgatct cttcttgtta cttgaaactg cttaccactg tcacgatctc
     4861 ttggtaaacg gctttttcac aacctctttg gttaagttga acgcacatcc agcacagaat
     4921 agttccgaac atcaaatcgg tttcggctgg aagaatattc aatcggctgt ccatctttga
     4981 gatagacgac ttgctgaact tccagtacag gatcgttttc tgcacagttc aaatattgct
     5041 gatcatattc atcactttta tctgcagaaa tagcacgata agcccctgca aaatggacat
     5101 tcaattcttg atgaagatga tcatagatag aggctaacaa atgttctttt tttaaacccg
     5161 gcacaagatg gactggcata aaggtatgct ctaaaacata tggctcgcca tctaagatcc
     5221 ttaaccggtg aatattgtag acaggttgct cggcatcgat catcaatcgt tcttggatat
     5281 attgattagg gaattcaaca tcaaaatgaa tgatttgact cttcaaagaa cgatgtctgc
     5341 gtttcatttg gagactcaaa ccttcatact cgttagcggg cgatgtgtct ttttgcataa
     5401 atggatgatt caagattttc gttcctgatc ctcgctgcga atagacaagt ccttccattg
     5461 ctaagatatt gatggctttt ttgattgtca tccggctcgc accaaattct tttaccaagt
     5521 cgacttgatt aggcaaaaaa ctatcgagag gatactcttt tgaacggata cgttctctta
     5581 aaatatttgc gatttcttcg tattttggca cgttattcac ctcttctaga gtataacgaa
     5641 agattatgct tccgacaact cgaagtagca tctggaacat tgtttattcg aaaaaagagg
     5701 tcatgacaag tttttgtcac aaccccttaa tgatctatta agcaatatct aaatctggtt
     5761 gtttatctag tactttcagg aatgggtacc agataaaggc aacgatgaca aagtcgatga
     5821 cttgcagcat accaccaagc catgaatttg ttgctaagac accgcttgca atgattggca
     5881 cagtccatgg aacagagacg ccagttggtg cagggacaag tccagcagcc attaccaaat
     5941 aattgaacgt cgttacgatt agaggtgcca caacccaagg aataaggatt gttgcattca
     6001 atacgatcgg tagaccgaag atcgctggtt catttacatt gaaaatacca ggtgctaaag
     6061 ctaatcgtcc aacatcacga tactgtttct ttttaagaac aaaggccatc aataaaacga
     6121 cagctaaagt cattccagag ccaccaatac caacagtgaa ggtttccatg aatggttttg
     6181 tgatgatgtg tggtaattct tttccatgtt gataagcttc taagttatca agcatcagtg
     6241 tattccaaat cgggtccatt acagagttga cgatgatttg tccgtgaagt ccgaagaacc
     6301 ataagaattg gacaaagaac aaagcaatca atgttgcagg taaactactt cccaaaccag
     6361 ttaatggttt ttggatcacc tcataaacga cgtcatgtaa gttagcattg aaaataccag
     6421 tcatcacagc attgattact aagaaaatag ttagtgtcac tacagcaggg atcaaagcgg
     6481 caaatgattt tgttactgca ggcggaactc catcaggcat tttgatctgc cagccgcgtt
     6541 tcgtgattct gcagtagatt tctccagcca agaaagcagc gatcattcca atgaacattc
     6601 cttttgcgcc aagacgatca agtgaaagca caccagtcac atcttcaccg ccagcggttt
     6661 tcattgcaaa aggcgtaaga atcaagaagc tggcaaaaga cacggcacca ccgaaaatac
     6721 cttcaacgtc ataagatttt gataaataat agccgatacc aaaagtaaca aaaaccgaca
     6781 tgatgctcat tgttgcattt tgcccattac cgaataaatt cgataaagtt ccctttgttg
     6841 cgtcactgaa aaaaggcaag ttattgataa cgacaacgat cgaaccgaac atcgttaaag
     6901 ggaaggaaag catgaaagca tcccgcagca ctgtaaggta acgattttgc cctaataaat
     6961 tagctaaggg catgattttc tcagaaagct tgtccataaa accgttcatt tttattcacc
     7021 tcaaatttat ttgcaatcga ttacatacaa agtataacgc caaaaaattt aaatgtctag
     7081 tcattttcat aaaaagtatt gatttatttt ttagggttcg ttatgattac ggtatcaaga
     7141 aaaaagagag gaagtaaaaa aatgaaaacc tacacattcc cagaaaattt ttggtggggt
     7201 tctgctgcta gcggaccgca aacagaagga agagtaagtg atgatggtaa aggagaaaat
     7261 atttgggatc attggtatga attagagcct gaaaaattct tcgatcaagt aggacctgaa
     7321 aaaacttcgc aagtttacac aaaatacaaa gaagatgttc agttaatgaa agaaactggc
     7381 cataacacat ttcgtacatc tatccaatgg agtcatttga ttccagaagg gacaggtgaa
     7441 atcaatcaaa aagctgtttc tttttatcga gattatttta ctgaattgct ggataatggt
     7501 attgaacctt ttgtgaacct ttaccatttc gatatgccac taccactgca agaaaaaggc
     7561 ggatggttat caagagaaac ggtcgacgct tatgtttctt atgcagcagc gtgctttgaa
     7621 ctgtttggag atctggtcaa aaaatggttt actcataatg aaccaatcgt acctgtagaa
     7681 ggaggctact tgtatcaatt ccactatcca aatcatatca atatgaaaga agcggtccaa
     7741 gtaggctatc atgagaatct agcaagcgca aaggtaatca aagcctacca tgaaggcgga
     7801 catgatggtg agatcggaat catcttgaat ctgacaccaa gctatccgag agatgaaacg
     7861 aacccagaag atgtaaaagc ggcgaagatc gctgacgcat tcttcaaccg atcattcctt
     7921 gaccctgcag tgaaaggaga gtttccacaa gagttgatcg aaattgtcaa agaattggct
     7981 ataatgccag agatccatga aggagaccta gaactcatcc gtgaaaacac gattgacctg
     8041 ctaggaatca attactatca gcctcgtcga atcaaagcaa aagaaactcc aatcaatcac
     8101 gaacatagcc ccatgccaga tgattatttt gataattacg acatgcccaa taaaaaaatg
     8161 aatccatatc gtggatggga aatttacgaa aaaggaatct atgatatttt aacgaataca
     8221 agagaaaact atggtaatat caaatgctat atttctgaaa atgggatggg ggtcgaagga
     8281 gaagaacgat ttgttaacag cgaaggaatt attgaagatg attaccggat cgagtttgtt
     8341 acggatcatt tgaaatacgt ccatcaagca atccaagaag gaaccaattg tgtgggttac
     8401 cacatgtgga catgtatgga caactggtct tggacaaatg cttataagaa tcgctatggc
     8461 tttatcgctg ttgatttggc acaagacggt aaacggacga tcaaaaaatc aggtcgctgg
     8521 tttaaaacgg tctctgaaaa aaacggattt gatgcctgat attttttgat aaaacgttca
     8581 caaaatgttt gttctaacac tgtaatcgtg ctacactaac agtgaaggag gcgtttacaa
     8641 tggtagatac ttatcgtaat attttagtag caattgatgg ttctgaaaag gcagaaaaag
     8701 catttcttga ggcaatcacg attgccaaac gaaatcaggc aacgttgcac atcctgtatg
     8761 tcaatgaagt aacaggcaat tactttggtg attttgcatt tgttacaaca agccttcagg
     8821 aagaattgga tgaggttgcg gaaaaccaga tgaaagaaca tcgaaacttg gcaattgaaa
     8881 aaggcctgac agatatcgag acatatgttc tttacggtta tccaaagaca ctgattgcta
     8941 atttccatga atcgaaagaa ccaatcgatc tgattgtcat gggggcgaca ggtctgaatg
     9001 cggtagaacg tgcactagta ggttccacta cttcctatgt agtcaaccac gcatcatgta
     9061 acgtcatggt tgtaaaataa ataaattaag ggaacagaaa gcaaaaaagc ataacgcttc
     9121 ttttgctttc tgttcctttt atttttttgt aaaaaattac tttacctgat gaagtaatca
     9181 tgctattcta attacaacga cagatggagt aatatggagg aaagaaaatg atttcaagtg
     9241 atggaatacg cggatacaat gatgcgatta ttttatcgat ccttaacaaa aacgattctt
     9301 atggatatga aatttctaaa acgattcgtg agaggacaaa tgattcctat gtgatcaaag
     9361 agacaacact ttactccgtt tttgcccgtt tagaaaagaa tcaactgatt gtttcgtatc
     9421 ctggagaagt gactcatgga aaaaagcgga catattataa aatcactgaa atagggcgta
     9481 atcttttaga agaaaaaaag caagagtggc tgacgacgaa agacgtagtc gatcaatttt
     9541 tggagggaaa ttaaatggat acaatccgta attatttaga cagtcttttt atcggagtac
     9601 ctcaaagtac cgagatagat aagctgaaaa ctgatttgtt ggcaaatatg gaagaccatt
     9661 atcatgaatt gatgggagaa ggaaaaaatg aacaggaggc catcggtaca gtaatttcga
     9721 catttggttc aatcgatgaa ttattagaag aactagatgt cgaaaaaaaa catcaagctg
     9781 acgagacgga aacaaacaca gcttctattt atttaagcga agcagaaaac tactggaaag
     9841 aatacagagc ggcaagtctc caagtggcat caggtgttct attcattagc ttatcttttg
     9901 caagttttct tttcttttgt tcagcaggat atgtgttcat gggtatttct tgtctgattt
     9961 tcggtatagc attagctgtc ggttttttta ttgcttctgg tatgaaaatc acacgtttaa
    10021 atcacttttt gcaccatcgg aaaatacctg aaaaagtatt agcagaagca aaagaaaaag
    10081 aagaagagta tcagcgaagt tttggcttct cactgattgc aggaattgga ttatgtattt
    10141 tttcactttt cccacttttg gcttctttaa tgtggtatat ggatggtagt atcggtgcct
    10201 ctatattttt tgttacggta ggaacgggtg tcttcttgat catttatggc agtctagtcc
    10261 gtcattctta tcgtcaattc actcaaagcg cttattactg gtgaatgtat tcagatttgt
    10321 aaaaatattc tatttatccg gcgtagaaaa aaagactgtg acacgctttt gtcacagtct
    10381 tttttatata taataattta aaaattcatc atctaaatcc gtagaatttt ctagattgct
    10441 tacccatgta aaatcgttat agacagattt cagttgactt ctgcgccatt gtacagggat
    10501 aaaaagacgc atgctatctt cattgaatcc gactaataat ttctttccgt cagtaatgat
    10561 gggacgttgt aacaaagtaa aatgcgagac gatcagatcg acaagctctg aggtcgtaca
    10621 ttggtcgatt tggtctttca aatttttata atcaatcgag cgtttagcta atagatcgtc
    10681 aaatccgttt tctgttaatg acaataaata aaatatctct ttccttgtta aaggctgttt
    10741 aggtgtgaaa attcgttctc tgtaaggcat ttgatggtct ttcagccagt ttgccatcag
    10801 atggtaacta tgtgtgcgag aagaatagat cgtaagcata aaaaaactcc ttaaatagtc
    10861 cattaatttg ttagatctgt tgagataaat aataggtaag gagctgtgca taagcattga
    10921 tcaaaacaac ccaaacatca ttagaaataa ctttctaaag tcttaaactt gcgacgagaa
    10981 acaactagac taatagaaaa aatgtcttag ccatttggct tcctgtttgt ttttttgctt
    11041 atgctgcagc tcctcgggaa agggttctaa caatttgaag aaggttgatg attgatctag
    11101 tttaaattgt gtgaaatttg aaatctggtg tttttactag atgaatcacc taacaactat
    11161 gattataact tatagaactt ctgttttgca ttcggttttg ttatagttgt tataaagttt
    11221 tattttatta cagaacattt ttaattaata aagaacagtt agaagttaaa attgctgatt
    11281 cacatagagt ttatgacttc ttggaaattc tttttcaatc agttttttca tctgtttatg
    11341 aaattctttt ttttcagtaa acataaaggt ttgggtatcg taaccttctt catgtaggaa
    11401 atagattatt cctttttctg tgttttgcgt ataagctttt atactcctgt cttttttgaa
    11461 agaacagatc gtatagccat ctatttggtc tggtagtcgt ttgattttct tcaaaatgac
    11521 ttttttatcc tcaagtttca tttttttcac ctgactttat ttttttcata tggaaatcat
    11581 agcgcctttg gttatatgaa aacaagaagg atactgaaat atatataaat caaatagtgg
    11641 ttatttatta ttagaatagc gaatacaaat aaacgaccac tattttctga tttattgcaa
    11701 atgttttttc aaataaattt gattaccaag aaaaggtagt ggtatattta ctgagggata
    11761 ctttgaaaaa catatgatag aaacagtaac agtattatga aaaatctttt tataaaaatg
    11821 gaggaatcga tttgatggaa gatcagtatg tacaagcaat gacgatcgca ggatcagata
    11881 gcgatggaag cgcagggatg caagcagata tgcatacatt ctttacaaga aatgtttacg
    11941 gtgtttctgt catgaccgca tgtgttgcag gtaattctta cggaatcgga gcaagtgtga
    12001 cattgccaac agattttatc gacaaagaat ttgaactgat tgcgaaggac tttcaaatac
    12061 gtgcagctaa aacaggtatg ctagcagatt caaagttgat tgagacagtt gtaaaaaatt
    12121 accaaaaata tgattttgga ccattagtcg tggatcccgt aatcgtaacg aaacatggaa
    12181 atcttttact agaagaatct gctttgcaaa gcttgaaaga aaagcttgtc cctttggccg
    12241 aagtactgac acctaatttt tatgaagcag aaaagctgac tggacggaca ttagcagctg
    12301 aagaagattt tgtgcaagca gcaaaagaac tgcaagatat gggtgcaaaa aacgttatga
    12361 tcaaaggaca tcatgctaaa aatgcgcagc aagaaacagt caatgactac gtattgttcg
    12421 aatcaggaaa atcattttgg ttaaatgcac cattttatcc taccgaccgg atcaacggaa
    12481 ctggcgattc gctatctgca tgtattgcag ctgagttagc gaaaggaaaa acagttgaac
    12541 aagcgatccg attggcaaaa gactatgtaa accgtgcaat tcgtgaagaa ataaatgtag
    12601 ggcacaaata tggccccatc aatcattggg cagctggtga attagagtaa aagaaagaag
    12661 ttgtgaaaaa gttgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
    12721 gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc gtttaagaaa
    12781 gaggtcgtga gcctgccgtc cagctccgag tcacaaggaa caagttcaaa aaacagcttc
    12841 tcatgtttgt tgaatattgt gacttgtgac cgaggagttg gcaggagaac accgtttgtt
    12901 cggaagaagg gactgtgaca agatttttgt cacagtccct ttaatcttag tatatttttc
    12961 tctacttgaa taaaaaaatt ataggattgg agaaaataat cgagaaattt gttgcttgaa
    13021 taatagccag ttagacattt ccttgactgt ttcagttgtc atggatgaac aatgtgtctc
    13081 atcttctttg aactgagtag caagttcttc taaaaactca ggatcgtaaa tgacagcact
    13141 tgtttcaaaa ttcaatcggt agctacgaat atcttgattt gctgaaccta ccatgcagat
    13201 ttcatcatcc attatcaatg tttttgcatg gaggaagccg ttctcataga caaggatttg
    13261 tacattttcc ttgatcagct gccgagcata atattgtgtt gctcgataaa taaaaggatg
    13321 atccggtttg ttgggaatca tgattttcac atctacacca gaggcagttg cgatttttaa
    13381 agcagcaatg acactttcat caggaacaag gtaaggtgtc tgtatccaaa cacgtttttt
    13441 agcagaagta atcaatttga taaatgaaag cttgatttgt tccctttcgt tattcggtcc
    13501 gcttgaaaca agctggatgg atgtatcagc aagcttagat tcatctcgat ctgctttttt
    13561 aaagaaataa ttcaaatgat agccgacctt tttttcttca ggtaccgaga cgttccagtc
    13621 tgttaagaag cgaagctgga gcaaagaaga agcggcaccg aaaatccgta tatgcgtatc
    13681 gcgccaatag ccgaattttt ttgttgtatt tacatattga ttggcaatat tgaaaccacc
    13741 ggtataacta atctttccat cgatcacaac gatttttcgg tgatcatggt aattcaatcg
    13801 gaaacgaagt aatgcccttt gtgaggtaac aaatgtatgg acatgtccac catttttgat
    13861 tagacgattg aaatcttttg cttttgtgcc atgagagcca aatgcatcat ataatattcg
    13921 aacttccacg ccttctgcgg ctttttcttc taaaacatgt aagacttgct ggccgatatt
    13981 atctgtcaca aatgcgtaat attcgatatg gatcgaatgt ttggcttttt ttagatcttg
    14041 aagcagtgaa tccaatttct cttgtccgtc tgtgagaaga gtgacagaat tcattcttgt
    14101 cagcggcata cgatttaatg aagagaagaa gtcaacaaat tgctgtttgt ctttatcgcc
    14161 catgtcagga tcgtaatgtt caatggtatc tcctctaaaa gactgaaagt tttctaattc
    14221 tttcaagtca ctttgtcgga gatagaattt tttcttatcc gttaatccac gtccgaaaaa
    14281 taaatataat acaaagccca ccccaggaag ggcaaacaat actaagagcc atgcccaaat
    14341 ggctgctaca tctcggggtt tgatcaaaat agccaccaaa gcaataagtg catttaatag
    14401 ataaagggcg gttataatac tagataccac aaaaatgttc ctccttacat acaataacat
    14461 tagtatagca tgatagaaga aagaagccta ccaaggtgaa tatacttttc actcctttca
    14521 tgaaaatgta ggtaaatcgg gctaaagtag ttgctttttg taggttaata aggtaggata
    14581 ttagtacagt tgaatatctc cttaggggag taaccggcaa cgtttgttgc gataatatca
    14641 acagtaagtt aaccaaatgg ttttctggta ttgtatgaaa tggtgagacc taagacaaat
    14701 gcgtatttgt cttaggtctc ttttttttca gacaggacta gggccaagat caaaaaatgg
    14761 gggatgaaat ttggaaaaca aggaacaagc acaacaagcg gtctataaag aaacgatcga
    14821 ctcgttgatg cagcaaacgc agagctcaac cgatggattg agcaccacgc aagcgaagga
    14881 aaaattagaa agagatggct tgaaccagct aaaagaaaag ccgaagaagc caacttggaa
    14941 attgttctta gaaactttca aagatgcaat ggttatcgta ttgctgattg tcgcactgat
    15001 tcagatggtc atgggtgact acatcgaatt cttagttatt tttgcagtat tgatgttgaa
    15061 ttctataatc agtgttgtcc aaacaaaaaa agcagaaggt tctttagaag cgttgaaaaa
    15121 cctttcagcg cccgatgctc gagtgattcg tgatggtaag gaaaaaacga ttccggcaaa
    15181 cgaacttgtg gtaggagata tcgtgttgtt agacgcaggc gattatgtac ctgctgatgg
    15241 acgtttacta gatgcgggat cattaaaagt agatgaaggt atgctgacag gagagtcgac
    15301 ttctgcagaa aaagaagtaa cggatatcac aagtacggta ccaatcggag atcgtacaaa
    15361 tatggtattc agtggaacga tcgtaactta cggacgcggt aaattcttag tgacttcaac
    15421 tgggaataat acggagatcg gacaagttgc tggccttttg gaatcaacaa ctgagcaagt
    15481 cactccgtta caacgaggtc tggataaatt cagtaaaaaa ctaagtttgg ctattttggc
    15541 attgtcttta ttgattttag gtatccaact gtttcgtatt tatcttggag aaggtacagg
    15601 agatatgact gcagatatcg tcagcgcagt gatgtttgcc gtagccgtag ccgtagctgc
    15661 tattccagaa gcattacagt ctatcgtaac aatcgtgtta tctttgggaa cgaataaaat
    15721 ggcaaaacgt catgccatta ttcggaagct tccagcagtt gaaacacttg gttcgacaag
    15781 tatcatctgt acagataaga ctggtacatt gacacaaaac aaaatgacag ttgttgacta
    15841 ctttttaggt aatggcgata ctggggattt cacaaatgac ccttcaaaat ggacagctga
    15901 cgaacgtcgc ttgatcgaaa tcagtgtttt agcgaatgat gcatcgatca gtgaagacgg
    15961 tactaaattg ggcgacccta cggaagtagc gtttatcgac tttagtgaaa aattaaacca
    16021 gccttatcag gaaatccgaa acaactaccc aagacaagca gaacttcctt ttgactcaga
    16081 tcgaaaatta atgtctactg gacacacaat tgataacaaa aattacttgt ttgttaaagg
    16141 cggaccagat atcgtcttcc aacgcagtaa aaaagtattg ctaaatggtg aagtcgttcc
    16201 gatgacggat gaattgatga aaaaattcca agagcaaaat gaagcttttt caacacgcgc
    16261 attacgtgta ctagcttttg cttaccgtcc acttgataat tttgatttat catttgaaga
    16321 tgaagacgat ttgatccttg ttggtttgct tgctatgatc gatcctccac gtgctgaagt
    16381 gactcaggcg gtaacagaag caagaagtgc aggaatcaaa acaatcatga ttactggtga
    16441 ccacaaaaca acagctgttg ctatcgcaaa acaaatcggg atttttgaag aaggaaacct
    16501 ggctttgacc gggacggaac tagatgaatt gacagaagat caactaaacg aagttttaga
    16561 aaaagtcact gtttatgcac gtgtttcacc tgagaataaa attcgtatcg ttcgtgcttg
    16621 gcaaaataaa ggtcatatct cagctatgac aggtgatggt gtaaatgacg caccagcatt
    16681 gaaacaagca gacatcggaa ttgctatggg aacgggtaca gatgtggcaa aagatgcagc
    16741 agcgatggta ttgacggatg ataactttgc ttctattgtc agcgcagtag aagtaggacg
    16801 taatgtgttt gataatatca agaaagctat ttcttatcta tttgcaggaa atttaggtgc
    16861 gatcattgcg atcatctttg ccttagttgc tgactggtcc aatccattta cagcattaca
    16921 actgttgttc atcaatttag tcaatgattc actgccagca attgcattag gtacagaaaa
    16981 agctgaacct gctacgatga accgcccgcc aagagatcca aattcaggaa tctttacgga
    17041 caaaacattg atttctgtaa cgtatcgtgg tgtgctgatt gggattgctg ttatctttgc
    17101 tcaatggctt ggcatgcagc attcacctga attcggtgta gccatgtcat tttcaacatt
    17161 gatttggtgt cggacattgc aaacattccc agcacgttca aacacacaaa cagctatcca
    17221 agcagggttc ttctctaaca gaagtgtctt actagctgta gttgtatgta gcgcgcttta
    17281 ctgcttaaca atgatcccag gactgaaaga agtcttctct atcccatcag catttggatt
    17341 gacagaagtc tttacatgta tgggaatcgc acttgtgaca attgttttga tggaaatcac
    17401 taaattgatc attgtacgag tacaaggaaa taaataaaac gaatcaacga ggttgtgagc
    17461 ctgccgttta attctgagtc acaaggtgca atttcaacaa acagcttctc atgtttgttg
    17521 aatattgtga cttgtgaccg aagagttggc aggagaacac cgtttaagga agaagttgta
    17581 aaatcagcat tttgagctaa tactgagggt catctgattg gacactgttt attcggaaag
    17641 agggactttg acacgacttt ggtcacagtc ccttttttta tttatgatag acggaaagcc
    17701 aatcatttta ttattcatca aaagagatag aatagataaa agaggagaat gatttattcc
    17761 gaaaagtgaa aacatataac aaaaaattag aggaaatcaa atgaatgaaa aagaatggga
    17821 tgaatttgct gaagaatatt atgaaatcca gcaagaatcg caagtaacta ttgctgaaga
    17881 tgtaaaaaat tatctgaaaa cggagaaagt cctgcctgct gaaacagcag cagacgttgc
    17941 tggaggatct ggcagatttc tttccttagc aaaagaaatt aatcgctatg atttgatcga
    18001 tttttcttca ggaatgctag aaaatgcaaa aaaagaagca gaaaaaaatg agttgccaaa
    18061 tgtttccttt atcaaacagt cgttagaaga atttatccaa tcaaatgaga catatgaaat
    18121 ggtttttaca gcagcaaatc cagcattggt acatcttatt caattagaaa aattgatgaa
    18181 aaagtcaagc aaatggtgcg tgataatcag agtagtggaa tcaatcgatg atgtttttta
    18241 tcctatcgaa aagcaactag gaatgataga agaagatcct aatacatcgc cagttgtaat
    18301 ggatcaattt gaaacatact taattgatga aggattcaaa gtgaagaaaa aagattttac
    18361 ttattttaca caggaatcaa ttactgaaga attgatgaag ctatactatg acgcgtacgc
    18421 aaaagatcct gttgtgacca ggtacattcg caaactattc agtgaaaaac gagaaatcct
    18481 tagtacgact aagctgacct atagactgct cctttggtcc gcagataaaa aaagaacctt
    18541 tcacgactcg tgaagggttc tttttatgat tttattatat tttgtagtaa cgaatatcat
    18601 tttcgatctt tgtaatcgaa aaaggtacat ctgaagtata ttctgcacat tctccgtgtt
    18661 caggaagcgt ctcactgaaa atcgtttcat attcgtcaac agtgatccgt gttcgagaat
    18721 ctagcatctc ttgatgagat tgtgcgaaga gatattcttg atagttttct tccagataac
    18781 ctgtaaaaaa ttcacttact gcaccagaac cgtagctgaa tagaccgatg cgatctcctg
    18841 gctgtagtga tttcgaattt tccagtagag aagttagccc caggtataat gaaccagtgt
    18901 aaagattacc gattcgtcgg ctgtaacgga tgctttcttc atagcgagcc ataagacgtt
    18961 cctgattttc ttcgtctgtt tggtctaata cgctttgcaa tgcctttttt cccatcttag
    19021 tatacggaat atggaaagca atcgcctgat aatcttcgag tcctcgaccc gacaattctt
    19081 tatgtcgatt ccaaactttt tggaatgatt cgatatacgt agaattagat aaaggaccat
    19141 caacaacagg aaattcgcta taatctggac gccagaaatc atagatatct tctgtcagaa
    19201 atacgctgtc gtcttcaatc gataaaatac gcgggttttg agtgatcatc atcgcaacag
    19261 caccgacacc ttgcgtcact tcaccaccgc ttgccaagcc gtaacgagca atatcacttg
    19321 ctatgactag tacttttcgt tctggatgat ttttgacata ttctttcgcc atgtgcaaag
    19381 ctgcggtgcc accgtagcaa gcctctttta cttcaaaaga acgagcaaaa gattggattt
    19441 tcagtaaatg gtggataatt accgcactcg cttttgaatg atcaatcccg gattctgttc
    19501 cgacgatgac catgtcgatt agctcacggt ctttttctgt tacgattttg ctggcagcat
    19561 ttgctcctag tgtaatgatg tcttcatttg cacgattgac tgccatttgg tcttgaccaa
    19621 tccctatatg gtattttgca ggatcatccc cacggctttc tgccaactct gtcatatcta
    19681 aatataaatt aggaataaaa aaggaaagac gatcaatccc tattttcatt tcaacacctc
    19741 tttacttatg ataaaaaact tactttacta agg
//
LOCUS       DAJSVF010000048        19633 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSVF010000048 DAJSVF010000000
VERSION     DAJSVF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.049"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            123..1382
                     /locus_tag="ON550_001720"
     CDS             123..1382
                     /locus_tag="ON550_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR3954805.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGDFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1470..2417)
                     /locus_tag="ON550_001721"
     CDS             complement(1470..2417)
                     /locus_tag="ON550_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3954806.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDHRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2811..4103
                     /locus_tag="ON550_001722"
     CDS             2811..4103
                     /locus_tag="ON550_001722"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR3954807.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4118..5887
                     /gene="aspS"
                     /locus_tag="ON550_001723"
     CDS             4118..5887
                     /gene="aspS"
                     /locus_tag="ON550_001723"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR3954808.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5996..6871
                     /locus_tag="ON550_001724"
     CDS             5996..6871
                     /locus_tag="ON550_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3954809.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7137..9020
                     /locus_tag="ON550_001725"
     CDS             7137..9020
                     /locus_tag="ON550_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3954810.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9095..9379)
                     /locus_tag="ON550_001726"
     CDS             complement(9095..9379)
                     /locus_tag="ON550_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3954811.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9369..10103)
                     /locus_tag="ON550_001727"
     CDS             complement(9369..10103)
                     /locus_tag="ON550_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR3954812.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10232..10855
                     /locus_tag="ON550_001728"
     CDS             10232..10855
                     /locus_tag="ON550_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR3954813.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            11047..12168
                     /locus_tag="ON550_001729"
     CDS             11047..12168
                     /locus_tag="ON550_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR3954814.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12175..15303
                     /locus_tag="ON550_001730"
     CDS             12175..15303
                     /locus_tag="ON550_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR3954815.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQVILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15322..18516
                     /locus_tag="ON550_001731"
     CDS             15322..18516
                     /locus_tag="ON550_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR3954816.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18662..19369)
                     /locus_tag="ON550_001732"
     CDS             complement(18662..19369)
                     /locus_tag="ON550_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3954817.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6547 a         3469 c         4156 g         5461 t
ORIGIN      
        1 tagactgaaa aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca
       61 gcagtcggaa aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga
      121 agatgaaaga agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa
      181 aaggatacaa catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa
      241 agattttcaa taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg
      301 atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg
      361 gtcaaggtgg agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta
      421 tggatctaat gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga
      481 cacaagcact tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt
      541 tctttacatc tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg
      601 tcgaaaaatt agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg
      661 atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg
      721 aagaagctcg acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca
      781 atgccacaga tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga
      841 acatcatccg tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt
      901 tcagtagacc agatttgtat cagcaattaa aaaatgactt ttcgacgttg caagatccgt
      961 ttattcaaga aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga
     1021 aagaagtaga agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat
     1081 atggaacaga ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt
     1141 atgtagggaa agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg
     1201 ggggagattt tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac
     1261 gtgtgttttc cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga
     1321 cgattccatt caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt
     1381 agattagcat aaacttatcg aaaaaaggaa gatcaaacag tgctgatctt ccttttttca
     1441 tattactata aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt
     1501 tgataatctt cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct
     1561 ggacgcttag ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa
     1621 acaagttgcg caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg
     1681 gttaaaattt ctctgaaacg ttctccttgg aaatctggat ttctttttcg atgatctcct
     1741 tcaccaaatt gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga
     1801 ggaaaatagg gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca
     1861 gcctgccgat ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc
     1921 gcttctttca attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct
     1981 tcttttaatt catgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag
     2041 tggatataaa aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat
     2101 tgtttgagaa actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct
     2161 tttgtagcga tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc
     2221 agttcttcgg aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta
     2281 ttcaaagctg tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta
     2341 tgtcctccaa cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg
     2401 attcttattt ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa
     2461 aagctgaact aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact
     2521 ggtttttagt aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa
     2581 actttacaag agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac
     2641 actctggaga tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa
     2701 gagaaatctt tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat
     2761 gaacaagggt gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc
     2821 aaagaccaaa aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg
     2881 aagaaacagc acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt
     2941 ttgaacacta tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag
     3001 agatgtatga tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag
     3061 caccaatcgt tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt
     3121 tcaaaacttt ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac
     3181 gtcaattcca tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg
     3241 agagcatggt gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag
     3301 tcatcaattc attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact
     3361 atcttttacc ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc
     3421 cattacgcgt attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat
     3481 ctatcttaga ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc
     3541 tggatgcatt ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt
     3601 acacacatac gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga
     3661 tttgcgcagg cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg
     3721 gcttcggatt tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag
     3781 ttatccctgc atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata
     3841 tcgaagcatt gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact
     3901 tcatgaaccg taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag
     3961 tattggtgat cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa
     4021 gccgaaaaga aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg
     4081 atgaaatgac tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg
     4141 cggattagtt tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa
     4201 aagacgagac cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca
     4261 aatcgtcttt aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag
     4321 tgaatacgtg atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc
     4381 taaaatggcc actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc
     4441 aaaaaccccg ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa
     4501 atatcgttat ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc
     4561 gacaacaaaa gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc
     4621 ttacctaggg aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca
     4681 tgctggacat ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa
     4741 tgctggattc gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg
     4801 agaccgtcag cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga
     4861 aatccaaact tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga
     4921 agtcacattg ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga
     4981 caaaccggat acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt
     5041 agaattcaaa gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc
     5101 taaaggcgct gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg
     5161 acaattcgga gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc
     5221 aattgctaaa ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc
     5281 aggtgacttg ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc
     5341 tatccggaca cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt
     5401 atgggtaact gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc
     5461 tcaccatcca ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc
     5521 aaaagtctat gcagaagctt atgatatcgt cttgaacggt tatgaactag gcggaggttc
     5581 tatccgtatt catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa
     5641 agaacaaatg gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc
     5701 acatggcggt attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa
     5761 tattcgtgaa gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc
     5821 accaagtatg gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga
     5881 agaatagccg ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga
     5941 tttaattgaa aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa
     6001 aaaaacattc gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt
     6061 ttatgcgttg atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc
     6121 aagtggtatt actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt
     6181 cgaagtaccg gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg
     6241 gttaaaaatc gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct
     6301 ttgtatccat attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt
     6361 tgggggggct gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg
     6421 cggtctagat atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat
     6481 ttcgatctac ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata
     6541 tatgttttac agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac
     6601 taaacaaaag aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga
     6661 aatccaaaac tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa
     6721 gcatgataag cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag
     6781 agaagcaatg aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat
     6841 acttggtcgt ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac
     6901 tgctgtagac agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag
     6961 aaaaactgct tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt
     7021 aaatttatgg gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt
     7081 cacttaatac ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg
     7141 aaaaacaaaa aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat
     7201 tagctgtggt gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt
     7261 tgggcgttcc gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg
     7321 aatgggaaca aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac
     7381 tctatccggc acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc
     7441 gagggctgtt aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact
     7501 ggtggcaaat caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta
     7561 cgcatatctg gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg
     7621 tattgttgat ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat
     7681 ccttcctctc cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg
     7741 tttattacgg taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg
     7801 tttggccaag tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata
     7861 tcgctggagg aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt
     7921 acaatttcat ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg
     7981 cagtgacagc acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt
     8041 ggattggcaa acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg
     8101 aagcaaaggt gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat
     8161 tgattctaat catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg
     8221 attatggtcg tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga
     8281 gcaaaccatg ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta
     8341 cggcaccgaa aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa
     8401 gtaaaaaatt agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc
     8461 aagcgatcct tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag
     8521 aactaacggc atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt
     8581 atccaaaagt agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca
     8641 ttgaaaaatt aaaggaacaa gatttgttga aagacactgt tctgatcgga ctaggaacga
     8701 atggctcatt ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag
     8761 tatactgggt caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc
     8821 ttgaacagat gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta
     8881 atgaccatag tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac
     8941 attacattca cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg
     9001 agacaaacgt gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc
     9061 tgaagttttt ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg
     9121 ttgagtgtgt ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt
     9181 ctgcttttgt tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc
     9241 ttcttttttg aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc
     9301 tagaaagatc ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa
     9361 aataatgttc atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata
     9421 ttgtaagtga tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct
     9481 tcaactaaca aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta
     9541 ggtgcaattc gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg
     9601 accatagtaa agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct
     9661 aacgtcgtat gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt
     9721 gttggcagcc ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga
     9781 acgacagata agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg
     9841 ttaagcgcga tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg
     9901 atagccgcct gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg
     9961 tcaacaatct tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa
    10021 gaaaaagaaa acactttgga actttggata atcttgatat cttctgcgta taattggtcg
    10081 attcgttcgt cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat
    10141 tatactattt ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata
    10201 ctatgaccat aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt
    10261 tcgagtacta ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc
    10321 gcaagatgag aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct
    10381 ggcggtagct gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa
    10441 tccagttctg cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc
    10501 aggaccaagc gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat
    10561 catgtttccc agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc
    10621 cagaatgtct aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga
    10681 tctgttcaag cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat
    10741 caagaaaacg gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga
    10801 caaattggac aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga
    10861 taagtttatg cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa
    10921 tgaaaacgag ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga
    10981 agacaggtgt catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt
    11041 gattgaatgc gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat
    11101 gacttgattg aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa
    11161 aaagtagagg caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat
    11221 gccgtagaat tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt
    11281 tttgctatat caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc
    11341 agttattcga actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt
    11401 aaaaacgtac aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt
    11461 gaagatgaca atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag
    11521 gagctatttg attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt
    11581 gtaaaatctg attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa
    11641 ctacttacac cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga
    11701 caggataatg ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa
    11761 aaaaaaggcg tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc
    11821 gaacccctgc atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc
    11881 ttttatcagt ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc
    11941 atcccgaata tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg
    12001 gcaaacggtc gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct
    12061 caaaaattag cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa
    12121 gaaaaatggc tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa
    12181 ccgaaaaaat tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca
    12241 cgtttgacag aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg
    12301 atttttgatg ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga
    12361 aaagagatgc gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt
    12421 gaacatcagc agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga
    12481 aaaggaaacg gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa
    12541 ggaaaagaaa taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg
    12601 atgaatctcg atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt
    12661 aattttttgg tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact
    12721 caatttttcc aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa
    12781 ttggatcatt tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa
    12841 gaggaaccag cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggtaata
    12901 ctcgaaaaac aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag
    12961 caactagaaa attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg
    13021 atattaataa acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa
    13081 cgatggattg ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa
    13141 gaaataaaag ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta
    13201 gcgatgacta cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga
    13261 caaacaaaaa tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt
    13321 gtcgaatcct tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa
    13381 gaagaattga atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga
    13441 attcttcaag aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga
    13501 cagttaaaat atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact
    13561 gggtcgctcg cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat
    13621 agtcagctat tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag
    13681 cagcaaattg ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga
    13741 tcaacggaac accccataca acaattaaaa cacgaacagc caaataagga agatatctta
    13801 aaagtagaac aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt
    13861 gccaaaagtg aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa
    13921 atcatggcaa cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa
    13981 aaggcatttc ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa
    14041 tctttagata gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga
    14101 caacaagcag aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa
    14161 aataatcagc tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat
    14221 acggatagta actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac
    14281 tcgaatcaac tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg
    14341 cttcggacac agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa
    14401 aaggaacaag aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa
    14461 gaaatctgtg catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact
    14521 tcttatcatg aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag
    14581 ctaactgaga cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt
    14641 atgatcgaaa taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa
    14701 aaaatctacg atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt
    14761 acgcaattac agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg
    14821 gaacggtatg tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc
    14881 caacgtttaa cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga
    14941 gggaaaaccg gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg
    15001 cataccctgt ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc
    15061 gatgtgatcc agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga
    15121 tttgggtcat tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa
    15181 agtgaaggaa gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta
    15241 caacaaataa gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga
    15301 taaaaaggga gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga
    15361 cgtcaatact tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg
    15421 tggcatgcag aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa
    15481 gagatagatt actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt
    15541 gctgtggaac tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca
    15601 atgctagaac cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt
    15661 ctaaaaacac aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa
    15721 acgattgaga ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc
    15781 ggttcattgg catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa
    15841 gcttacacag aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa
    15901 cgggcgtttc aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg
    15961 catcaattat taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg
    16021 cttctcccag tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg
    16081 catctgcaga taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct
    16141 ttatcattca aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt
    16201 gattttttct ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg
    16261 aaatatcaac aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca
    16321 gaagtagtgt tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg
    16381 tcagagatcg gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc
    16441 gtcgaattta ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc
    16501 gtctatggcg aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct
    16561 tttcaggtgt tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat
    16621 cgctaccaga aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat
    16681 gcctttttcc gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga
    16741 aaattgcgag agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag
    16801 accggttcat ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat
    16861 catgtcctgc aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt
    16921 ctttcttttg attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa
    16981 tcgatacgta cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa
    17041 aatcaattgg aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat
    17101 ctcattcatc cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga
    17161 gattatcaaa agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt
    17221 attttagccg atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg
    17281 gaaaaagaag aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac
    17341 aactggcagg cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg
    17401 aataaaaaag aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat
    17461 gccagcttaa gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt
    17521 gatgaggcac aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta
    17581 gctgtacctc agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg
    17641 tggtcattgt ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa
    17701 cttgccgcag aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa
    17761 actgtgttga aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa
    17821 gaacaaaaaa ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg
    17881 gattctgaag cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga
    17941 caaatttgct gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc
    18001 gaacaagtaa aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt
    18061 tctcaattta cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg
    18121 acattttatt taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt
    18181 aatgctggcg aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat
    18241 ctcacgggag cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa
    18301 caagctgctg gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg
    18361 atctctgaag gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg
    18421 ttccaaaaag tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc
    18481 cgcatgattt taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa
    18541 tgggtaagaa agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag
    18601 gctgggattc cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag
    18661 gttatacaaa gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat
    18721 cgcaaattgg agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt
    18781 tagcactttt tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt
    18841 ctggaagcat atgaaattct ttctcagcta gctgaaaaat ctctttttct ggtttttgga
    18901 aacctgttgc ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt
    18961 gcttcagttt ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat
    19021 tcttttcctt caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa
    19081 gttcttcctc gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt
    19141 ctaaatcttt tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc
    19201 ctgccattac ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat
    19261 gcatatctgc atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct
    19321 gctgatcata catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg
    19381 cctttcggtt ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc
    19441 taaaaaagct gataaataaa ggtttttctt actatatttc tatttccttg acagaataag
    19501 gtttttatat ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa
    19561 caaattaatc attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt
    19621 tcgaaagatt tat
//
LOCUS       DAJSVF010000049        18843 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSVF010000049 DAJSVF010000000
VERSION     DAJSVF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.051"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..176)
                     /locus_tag="ON550_001733"
     CDS             complement(<1..176)
                     /locus_tag="ON550_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954818.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
     gene            complement(343..2313)
                     /locus_tag="ON550_001734"
     CDS             complement(343..2313)
                     /locus_tag="ON550_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954819.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2328..3194)
                     /locus_tag="ON550_001735"
     CDS             complement(2328..3194)
                     /locus_tag="ON550_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954820.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3184..3417)
                     /locus_tag="ON550_001736"
     CDS             complement(3184..3417)
                     /locus_tag="ON550_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3954821.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3429..5585)
                     /locus_tag="ON550_001737"
     CDS             complement(3429..5585)
                     /locus_tag="ON550_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954822.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5605..5826)
                     /locus_tag="ON550_001738"
     CDS             complement(5605..5826)
                     /locus_tag="ON550_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954823.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(5936..6274)
                     /locus_tag="ON550_001739"
     CDS             complement(5936..6274)
                     /locus_tag="ON550_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954824.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6674..10315)
                     /locus_tag="ON550_001740"
     CDS             complement(6674..10315)
                     /locus_tag="ON550_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954825.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGELLKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10318..12267)
                     /locus_tag="ON550_001741"
     CDS             complement(10318..12267)
                     /locus_tag="ON550_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3954826.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12309..13367)
                     /locus_tag="ON550_001742"
     CDS             complement(12309..13367)
                     /locus_tag="ON550_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCR3954827.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13486..13941)
                     /locus_tag="ON550_001743"
     CDS             complement(13486..13941)
                     /locus_tag="ON550_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954828.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14420..15223)
                     /locus_tag="ON550_001744"
     CDS             complement(14420..15223)
                     /locus_tag="ON550_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3954829.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16181..17404
                     /locus_tag="ON550_001745"
     CDS             16181..17404
                     /locus_tag="ON550_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR3954830.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17439..18206
                     /locus_tag="ON550_001746"
     CDS             17439..18206
                     /locus_tag="ON550_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3954831.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            18398..18796
                     /locus_tag="ON550_001747"
     CDS             18398..18796
                     /locus_tag="ON550_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3954832.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         5688 a         3596 c         2735 g         6824 t
ORIGIN      
        1 aaaactctcg ctttttcttc atcagtaaag tttttataca actgattaca ataatgaggt
       61 ttttgaagtt tctgaatacc tatactaact tcaaatcctt cttcatgttg aattaggatt
      121 acttctgtgt ctctacctag aaatattcga tctaaaattt ttatgtttgg tttcattatt
      181 taacctccac tataattatt tcactcagta aggtttcact ttatgaaatt tctatagttc
      241 tcttttcaca aaacgtacca tgttctctaa gactgttttt agtctcaaat atacatgttt
      301 tttcttatcc tttccttata cactcaaaat caaagataaa acttaaagaa taaactctcc
      361 aataacaaaa ttattagtca gatccactgg tctcctgatc tgaaatttat tacttcttcc
      421 tgttgtcaaa tactgtctca aatcaacaga aagtttttga tcatcttctc cttttctagc
      481 aggagcaact tttttttcat acttcattaa ctctccactg gtcatctttc caatgatagt
      541 gtaattaaaa tcactttcaa aactttggta aatttttcca tctgtattga atatatcagc
      601 tgtttcaata tcttctttgt attcattgtc ttctcctgaa ccgattgtat caaatagaaa
      661 ggcaagacga attccatctt cttcaagcat tgttaatctt tttttaatta gcccaacggt
      721 ttcaatagat aacttatcga agccgtgaat cataacaata tcatcttttt ttgcttgtga
      781 tgtgatgaaa tcaaaggtat ttaaaaattg agcttccata acattggttt ctgctcttag
      841 atcacctaag ttatagtaaa actgtaattt gtcactttta ccgatttctt tcagtgtcgt
      901 tctttgatta aacatcctag agaagctgtc taatgctgtt ctcaatgtgt cttttaatac
      961 aataatacga tcgacttctt tttcagtacc agattttttg gcttttgttt cagcattagt
     1021 caaatagcta ataaaatccc caagaactgg cattgtatca ctactgttca ccaaaagttt
     1081 tgaacgttct tcataattaa tatcccaaag tttctgactt atataaaagt tacctaattc
     1141 ttttttcaat aaaactctag ttgcagaatc taatgttctg cctgctaaca aataaaaaat
     1201 ctgagttagt ttatcgagat tccgtctata taaatcacca atgtcctttt gattaccaaa
     1261 aagctccaaa gagttaagac cttcttcagc caaattaacc ttttctatta cactgtttaa
     1321 cgaaggtttg gcaacaaatg ttctaccagt tggatcacca aagtaatcaa atttatttaa
     1381 gacaatatga aaagttcgtt tatgatttgc cattgcatta ttagcaatca attgccccca
     1441 aagtgatgat gctgagattt tttcatcata gcctttaatt gtagcgtttt tgtgtgatgc
     1501 cactaaaact ttacttgtaa aagaatcatc cagtgccata aaagctacac cacttgcgta
     1561 gctagtagac atagtgccga tagcccatcc attcgcatca tctaaacctt tgcgaattgc
     1621 atgatcattt cctgcaaaat ccgaactcat agacgtatac tcatagacat tcccgattgg
     1681 ttcacttaaa agtctatcaa acagatcacg ttgatctcct ccatatgaca taagctttaa
     1741 tccatacatt ttttcatcat acaaccgttg gagtttaatt agctgttcgc tgattttatt
     1801 aggagaatcg gatactaata ggatttctag cttccaatca ataattttat ttgccatgct
     1861 gtctagtaaa gatgctgtat tcaaatccat aatccgcata acagttttgc taatttcagc
     1921 atctgaattt tttccttcta aattattttc ataactattc gttgatcccc caacattctc
     1981 gtgcattatt tcttgttgat ctttttgtga cactggctta tctgcttcaa ttagataggc
     2041 ttttacccct tctactctca cttcaggaat agctttaacc atccaaccaa atccataatt
     2101 tctattcatt ccataagtat tcactaattt cacaattgtt ccccaccgct tacctgtcct
     2161 aatgtaagat ggtgtaaatt gataaccaac taaagatgtt aaatcactaa caaaggtatt
     2221 attcactacc tttgaatttc tagttttttg ttctttttta gtttttctct tttttttgaa
     2281 atttaatagt tgtgatctac ccataatttc catttaatct ctcacctcta tttcagtgca
     2341 taaatatcag ataatatttc ttttgtttgt ttagctgaca actgagtgac actataataa
     2401 agtcctctac tcgtatagtt ttctaaacgt tccacacctt catccaatac catcttagat
     2461 tgagatttca agataagata ttggattact gttgttttca caccatcaat atattctgat
     2521 acaaatcgtt cttgctgatc tattatgtca aagactgctt tgttactcct tgcatttcgt
     2581 ttttgtattt tcatatttcg agtttgtctt tccgtattct gaggttgaga tgaagtgatt
     2641 ttgatttcag ctaaggaacg atctctggta ttttgatatt gtattgattt cgattcctga
     2701 gccataactt cttctggaaa agcggttagt gatgtagccc catctagatt gaacattaat
     2761 gctacactcc catctcgata tacgattgtt ccatcttgtt tcacttttaa aatctctgtg
     2821 cctcgaaatg gtttcttttt ccccatagat actcttgctg aattaactac tgatttgaac
     2881 agtctaatag aaccagaaat caatgaacca ctagatggat caataaacat atactggcta
     2941 attagaataa gaaatccaat tatcattaat aacgttggaa caaaaataaa tttcagttta
     3001 atcgtatcag aaactaagta aacaattgat ccaagaacaa cgactgcaac aactatagct
     3061 gatacaatta atctttttgc tgtaacagaa taggtgctat tgacatgctt ttcaagccat
     3121 tcaactctga caattgtttc agaaggtttt tgatgtaagt tatgtttaaa tccatagttt
     3181 gagttatctg ccatcagaaa tacttccttt cacgttcaac ttacggtaaa cacctacagt
     3241 tatcactatt gcaacaataa gcaagccaat taaagatatt gacttgctca ttgtctcgtt
     3301 cgtttgtggt agttcattta cctgatacac gagctgtgct tcctgtaagt gctgagaaga
     3361 ggtcatgttt tcttgattgt tttgaattac tggataaact attttttctt ctcgcataat
     3421 agccctcctt agttattatc agataagtga gcttgaattt tgtactgatt tccgtctgga
     3481 gttttcacaa ctgctttttc tccatcaata gaaatcactt cagccttagt tgttttccca
     3541 tcaatactga tgacaacatc tttcccaact ttgaactctg tagttggtgt tgcttcatag
     3601 ctactatttg ttgaagcaga gttcgtattt gtcccaatcg aattttccac tctcgaaata
     3661 gatgtttgag tttgagcctg tgcttgttga ttattatgac taataaagta ggacgatccc
     3721 aagatcgttc cgcaagccat tgcagcgaca gaaatgatag tagccatctt tttacttgta
     3781 aaatcacgtt ttgaacttga ttgattttct gtagcaactg ctaatgattt cgttttttct
     3841 gccatttgag ctagatcact tcgggaacga gataagtctt ctcttaaagc ttcactttgt
     3901 tctgccaact tttcgctatt taatttataa ccctctgcta aatctttgac ctctttcatt
     3961 cgatctttat attcttcgat agttttttgt tgctctacaa gttttgtttc taattctttc
     4021 gtgttgttag ctacctgctt ttcaacttct tctttgttcg caaaatattt gacaaccaca
     4081 tctgttgttt tctcttttgt atcatggaac tcagcttcat tttctgactt tgttttttcg
     4141 acaatctcta aagttttttc ttgcacgtct gttaatgatt tttcaatttc tggtttcaat
     4201 gaattgataa ttcgttccac cacaacttct tttgcttctt ggctttgttt caatgcatcg
     4261 tttttaatag attcaattgc ttcttggtac tgttgatcaa gactttcttc agttgctttt
     4321 ctatattcat cttcttgttg tttaacttct ggtaaatgct cagcatcata ccgttgctct
     4381 agtttcttta gttcagcatc caaataagcc tgtttcgcca tatcataatc tttacgaatg
     4441 atattggatt tttccatcac taaatcatca attttagatg cttctgcttc gtagctctct
     4501 ttagcaaccg atagtgtatt tgtaaactga ttttgaccat ttaatgaggt tgcttcctct
     4561 atccgcaaaa gctcatcagc taacatttgc tttcctttga ggatcaattg ctccttcgtc
     4621 ttatttaatt cactgttgat tttattatta gcaatagaga tgcgagcgtt catactctct
     4681 ttagctaaaa acagcaatgg tttcaactca ggtatggata gcatctcctc gtttaaatcg
     4741 acagggtcat taatctcatc taaaactaca ggttgaaatt tgtcagcaat gatctgttca
     4801 aaattttttt ctaaaaaatc agcatctaag tttttcggta catcactttc agatgtgatt
     4861 ttttcagctt cataaacata attttctttg tcactttggg attcgggttt ttcatcaatt
     4921 tttggttgtt ggtaggtttc tgtttgtatt tgtgtttctt ctatgcttac ttcttggttt
     4981 tctgcttcgg ttggtttgtt atcatccaca aaaagagctt ctttttcatc tggtacattt
     5041 tctgctacgg cttcaaattc aacttcttca gaagagttga aggtctcagc aattcgttct
     5101 aatccattag tagctacttc taattgctct actgcgctat agaagtctgt agcttcttca
     5161 acttctgatt caaacacatt aggatcatcc ggaataacta ataattgacc actactatcc
     5221 ataaaacgga cacctgctaa tagcttctct ttaaattttt ctggtagatc agctacataa
     5281 ttcaaacttg acttcaatag aacgacatac ttctcagaat cgagaatagc atcatcctca
     5341 tctataactc cctcactgtt gagcttttgt acatcttcaa ttaccatata atttttcttt
     5401 ggtaaatctt tggtcagcaa cgcatcgaat tctttccata cctttgcttt agcttttttt
     5461 gccttcgctt ctttttcttc ttttttgtac acttcgagta attgtggctt caaagagagt
     5521 gcaactacag gaactgtttc tgccacataa ttaatagatt cggtaatatc tttgaatttt
     5581 gccattattg taaaactcct tttttcagtc aatagttctt ttctgtttat ctagcatata
     5641 tacatatgct ttttcgaatt taataagcgt ttcaagtctg gctttttcaa tctttttact
     5701 cccatttcga taatcataga actggttaac attcatattc attatctttg ccagttcggt
     5761 acctttcaga ctgctcatta atacttcgtt ggcgtgagct aacatttttt ctctatcaaa
     5821 aaccatctta catttacttt cttccctcac ttctaaacta cgcttggagc gtagtttttt
     5881 ggtaaaaagt aaattgaaca aatcggcata ttatccaaat ttgttcaatt cacttttaac
     5941 ctaacagttc attttttgga aattttgaaa ctagatagat accaacaact ggaataagtg
     6001 ctgatatgat attagctttt tggttcaaat cattacctgt tttagtaccc attgtgtaaa
     6061 gcataacggt tgtaattaca ggaataataa agattgcaat aatcgcccca acatatttta
     6121 atccatctcc ccattttgca tcaccaaaaa atttcattgc agtgaatcct ggtttcaacg
     6181 aaaaaacgaa tgccaagaaa tacaaaacaa cttgtactaa agttttcata tcaggcgtca
     6241 ttccgctaac caaattttta atagaatcga acatataaaa acctcctttt taatattttt
     6301 aataagttaa ttatatcatc gacaaatctt atttgttcac ttgtcaatga tctaactaag
     6361 aaaaagacgt ctcgatattt acatctttat gttatcataa taacataaat tttcgatgat
     6421 taaaagagat tttccattaa attatttcac tttttttatt ggctaaaaat gctggtttat
     6481 caagtttttg tttagataat aattctgttc tttaccagca taagcccttt ttaattatac
     6541 aactttgaac agtaaatatt taattcattt atagacaatt taaaataatt tttttgcata
     6601 aatagaaatt ttccttgaca tcttcagtta gaaatcaaag taaaaagaag gagcaaatca
     6661 atgctccttc tttttactcg tcttcagact tattttcagt tgatttttga ttaccgtatt
     6721 ttacaatata gtccttgact gacatggtag atttcttcac tactttacca gttttggatg
     6781 ctcctgtagc agtagccttg gcaaccttag ccgttccttt acctatttta atagcttcgt
     6841 ttttagcttc ataggttatc cctgctagac taccttttgg agcttttgct aaagctttat
     6901 ctgctgaggt gtaaacattt ttaccagttt ttgatagata ttcaacggct gatttagctg
     6961 ttgatggttt cttggttgat tgattagatt tccctgaatt atctgaagta gatttagaat
     7021 tatttgttct attattttta tttgtcagtt caggttctct aattgattga ttgtctttcg
     7081 acattcctga tccaatattt gtttcatatt tatctgtttg attaacatta ggagtaggaa
     7141 tgaacttttt cttcacatct gattgttctt tatccgtttc ttttgttagc gattctctgt
     7201 ctttaagtgc tgtgtcattc cgaatagcac tattatcaaa gttgctgtta agctctcttg
     7261 actgactttc accagatgct ccatctccta attcccgatg cactctgtct gacacgtcct
     7321 caccactcat ttcattcctc agttcttgat gagtttgatc taaagtaatc tcgtcatctt
     7381 cattctcagg aattgcaaca gcaattggcg tatgtcgttg ttgttcgagt tctctatttc
     7441 gatctgccac aagcgcatct ctcgtctcaa tgatattccc atcctcatcg actaaatctc
     7501 cattcacatt atatccatcg ctgtgtttac caagtttatt accaaactca tcaacaagat
     7561 ttccatcttc atcaactaaa cgcccttttt cgtcaactaa acgtccgtta ctatcctgac
     7621 caaaatcacc atttagcaac ctgcgtccat tttcgtcaac aagatttcca tcttcatcga
     7681 ctaagcgacc tttttcatca attaaacgtc cttcgctatc cataccaata ttaccatctt
     7741 cactatcttc caatggttta ccattagtat ctataaggtt tccatcttca tcgaccaaac
     7801 gccctttttc gtcaataaag ttgcctttag catctttacc gtatttacta tcttttaata
     7861 attcaccatt ttcatcgaca aagtttccgt tttcatcaac catacgtcct aaatgatcaa
     7921 tatattgttg ggagttatta cgtagttgat cttgacgaac taaatctttg aaagaggtat
     7981 tattgattgt tgaaccaact ccgccaccgt tgaatgacat tccttctggt gtttcaggaa
     8041 ttactttatc agcaatggac ttagcatcag cattgctatc tcccatattg ttcaaacgtt
     8101 cagccatttt ttgcgctcca actgctccaa gacctcctgc aaccatatct tttactgagc
     8161 tgttgccacc atcataatag ggatttttag ctccttggct tttcttgatt gctttagaaa
     8221 cgccgtccac aactcgcaca gcatcatttc tattaccaaa taataactta gcacgttcat
     8281 caaacccaaa cgctacttca agtggtaagc caataagcat attaatcaaa ttatttactt
     8341 tttcgccttt tttaaagact aaaagatatc ctacacctaa aatcatagca gatcgaacaa
     8401 tatttgctac gccatcgcca actaatgtat taggaatgaa tcccacagca gtatcaccaa
     8461 atgcacgaat aaaagtaatc agaaagatag gtaattgtag tccgatcaga gatgttgtaa
     8521 caacacctaa tgaaattgcc atcacacctg ctcctgatgc aagtgatcct gttagaacta
     8581 cttcccaaat ccgaataaat tcacgtttaa atgctttcac gatattcaca ttgaacatcg
     8641 cccaagcaca tgctaagtaa agaactgcta aaacgattga ttcagcgttc atcgctagcc
     8701 acgagccaaa cttacccata gctccatgac caactaagga tgctgtacgc cattctgttt
     8761 ttactgttga catattctta gcactgcctt gaattcctga aggagcgata ttatacgcat
     8821 aaaacttagc tcccgatgta tcaagtgccg attggagcaa taaagctacc gactgtgtgc
     8881 taaaagttgc attgttagca acgccccaag gttgagtagc atcaaagtta tgcgctgttc
     8941 cttttccatc aggcttgcga ttgtcgtctg tcacattttg atttgctgac caaatatatc
     9001 catttaaatt ggttggtttc ttaattgcac ttccttcttt tccatcccct aaattatagc
     9061 ctgtccaaac agcaggcgct cttcccatat aagcatcatt ttcggcgaca ttaacatcac
     9121 cggcataagt attaatattg aaagtatcat tcgatgacca ttcttttaat agagccatac
     9181 cgactgaatt agaatctaga tcattatttt gtctggcata actgctagta ttgatcgctc
     9241 ttatcattgc agatttttta gatggttcat aatctgtatc aacgaacttg atttcggatg
     9301 taggctttcc gtcaggtaca tcaccaccag tatcagatga tggagcaaga ttttgagtag
     9361 aagcccaaag tcttgtgtta atgatgtagt cagaaacaac atttgttggt atatctccat
     9421 ctttttttgc tgactggatg ccgttgatat aaactcccaa tagcgatcct gccactggta
     9481 agaatataaa caatacaaaa atacgaacaa tccatttttc aagtactggt gataatccct
     9541 tcgagttaac accatctttc gacaataatt tcattccttg ataacctagt attgctgtga
     9601 aaaagagcat agataagctt gctatcgctt taaataattc attatttaat cccaaattgt
     9661 taagcaagct agttaatgct ttagataccg gattagatac atccgatcca ccttctcccc
     9721 accccataag agaaaatata ttcatactca tgaaaaattt tccaaggagt tcatttatgg
     9781 attcagcaat attagaaagg attaaactaa ttgatccgat aaaactacca atcgtcctac
     9841 caatacttcc tagaatggat gatgcttgac tggcaatagc atttcctgag actgtttcaa
     9901 tatatccgta gtaactataa gttgctaaag tagatgctag ttgagtacct gcattacccc
     9961 ctgcaatcga attaccttgt ttagaaatgt cagaataagg gaaatcggca ctgataccac
    10021 cttgtcctaa caaattcatt tgtccagttt tagcggcact atcattaact tctttatctc
    10081 ccattaggat acgagcatat agtgtccaca ttccttgttc tgccggttta ctttccgcat
    10141 atgcaacgtt cttatttata actgtttctt gtttgctatc ttgattgtca ggagttggat
    10201 cattcattcc aaaaggagcg cctaaagtaa ctgctaatac tcctccaata actagacctc
    10261 ttacgaccca tcgttttatt ctaaggtttt gtagtaaata tttcttgaat ttcatagcta
    10321 cgcccctcca atcgaaaata caatcttgtt ttctttactt tcagcaacgg aaggctttgc
    10381 aataatttca gcattagaat caccttttcc ttcaaacgct ccggcttcac tcaacttaac
    10441 atcgaatatt tttgcgtttg gataagtaat tattgcttca gtaatcgctt cttcaaactc
    10501 agattcaact aaatgcaaga tattccttgc accccctgat ttagaatcct cttgtgaagc
    10561 catgtcaaaa actaagtgca tggagagatc gtctgcataa aaattttgac catatactct
    10621 ttctttccta ggtggaagac gtaaatctat atttaaatgt tttcgtacta attcagcact
    10681 cttcaacatt tcattataag caatttccag aagagtttgt tcggataaag cccaaaatgg
    10741 aactattcgt ccaaatcggt taataacttc aggctttaga ccttcaccaa tcaaggcttg
    10801 tttgatccct gatgcgactt ttcggtattc tctttttagt tcagtgctgt attcattaga
    10861 attttccttg tttgtaacac gagatagtga gctatatcgg tttaacgaat cgaacatctc
    10921 cgcacctgca tttgttgtcg caaataaaat tgtatttgac aatgaaacgg tgtcgtgaac
    10981 ttctcgatca tcccatcctc tttcagtaaa acttatccta ccttcatcta aaattgataa
    11041 aaaggcatcg agtgtttcta caccagcttt ctcatattca tcacacacaa taatcgcaga
    11101 aggatcatgc cttacatgag tacctataag tcgtctaaaa cgcattgcat catctttggt
    11161 tttgtagtcc ggcatattaa ctaagagaac tttattttca tcgccataaa gtcctcttgc
    11221 cacggcttta gccattgcag ttttaccgac ccctgttgat cctgtaaaca gagcagttgc
    11281 ttttggttgc tttcgacctt taaaacgatc aaatccaagc ttcatacgtt taaacaacat
    11341 tgttatagta taaactgcta acggttgtcc ttttatggac tttaattcct cttgaagttt
    11401 ttccatatca aaatcagaat ctagctgaat attatactta ccaaatattt gcaatagact
    11461 ttctcgatta taagcatttc cgtctactcg atcttttgca atcaaatcct ccattacagc
    11521 aattgattta gccggttcgg acaaatcctc acgataaagt ttgtttgctt gtacgatttc
    11581 ttgaagtaat tcatcaggaa tcctttgcga taatctaaaa gaagcctgag gatcatcttt
    11641 aacagaggat gtatttagcc attgtctcaa aatatcgacg acaacatcgc tttttagttc
    11701 attcaattct aacggttgta gccgtctccc taaggcatca tctcttttga tatagttttc
    11761 atattctttt cttgtagtag cggtaatcat ttgaagtgat tcatttgcta acggttcttt
    11821 tagcgaatca cccccaattt tattgccact cccaaaagag ctaacaactt tatgaaactc
    11881 gtcaatgaag aggacaaatt cattatcttt gtcctcagct tttaaaattc gctcatattc
    11941 tttcaatctt ggaattaatg tttctaaacg tgtttggagg acatcatctc ccccaacacg
    12001 caattgtccg attgataatt tatatacttg tactttcttt ccatcttgtc gctgtctttc
    12061 agcccattca cttgcaagtg ctgttttacc tgttccggct tcacctaaca agatagcttg
    12121 cggtgtttcc ggtctattca agatcagtga caactcatgg agttcgtcat cacgtcccac
    12181 taacttatgt tttggtggtt tcattggtgt gagagcatga ttaaattcgg tttgaatatt
    12241 cttaaaaaac tcggtactat tttccatctg tttgtgttca cttcctctaa aataagaata
    12301 atttacttct agtcagtcaa agttaggtac ttaaatgtcg tcttcatgtc tccaaaagaa
    12361 tctttgttaa agtcagcatt tttgtattga tcaatcaatt tttgtgtatc attgattgct
    12421 ttaatttcgg tctccattcg actgtctagt tgacgaatac tttcttgcga atctttatcg
    12481 tcaggatttt gttgtaaacg ttccagatac tcagcttttg tggcttcaat tttcttgatg
    12541 ttagtatttg aagcatttaa ctgatcttct agcttattta caacattatt cttacttgta
    12601 actgctacaa cttttgaata atcaatatcg ccatctttgg taagaaagct tccttgatag
    12661 acattagttg aatctgaata tggatttatt ctgaaattga tattatcagg agtattaaca
    12721 ggttttgctt ttttcccaaa agaaattact ccttcattat taacatcatt catagaatat
    12781 tgagaaagtg cttttgtaac tgaatctgct tcattcacat tgactttggt atcatcgtca
    12841 gctgtatcat caccagaaat tagtgatact tggtttgcaa taaaaacttg ataagctctt
    12901 tcctctaatt taccttttag gaataagaaa gcatccccat cagtacttaa catcccatag
    12961 ctcatttcaa gcttcttagg acgatctttt tcttttgttg ccatatataa gttatagttt
    13021 tttccttgcg tagataacaa ctttctagcg ttttgggagt aaccaagttt tactactgtt
    13081 acatctctat tttgatctgt ccacacatct gaaatagtga tttctgctcc tgatcgagca
    13141 aactccaatg aagaccctat aggtgttgta ttgttcacga gataataatt atagtttcct
    13201 aattgataaa tgtagctacc caagcctgtt aataaaaaaa gcactcctag aaaagttgca
    13261 agcataatgc gtaatccgtg acgtgttctc ttatccttat caccaagcaa ccctccaata
    13321 atactgtatt gatctttggt agatgttgaa ttgacgtttt tttccatcag tttgtctgcc
    13381 ccctttcata aaagagcaaa caatcagacg ttttttcggt aattctaggt tcgggatttt
    13441 gaatcatttc ggtatattct tccccattaa tatttaacac aaaaattatc ttcctttctt
    13501 cgttcctttc ttttgctctt tattttccaa ataatcaatg atttttgcta ttgtaccaag
    13561 atagtaattc ccctcagctt tgttttttcg gattttgcta accattggag taggtacaat
    13621 ctcttcatct ctcaattcac tgaaagattt tttacctaga tattttccaa taacagattt
    13681 taaaaaacgt tcaaaatctt tctcgtaagt tgttttcttg ccttttagtt catggttcat
    13741 attgtcaacg acttttgcgg tttcaataat tgttttagtt ctcagcttaa taatttcatc
    13801 tggaattttc aatcctcgta atttcatcgt tgtactaact ggtaacccat gctttactgc
    13861 atcagcaata ctaaaacttt ttagatcatc tagtaagctc tccatatagt taacaaattc
    13921 atgccaatta atagcattca ttgtctcacc ccttcatttt tttaaaatga ggttcgggcg
    13981 taacacattt atgttattat aatatcataa taacataaat aaatcaatca gcaaatttag
    14041 atatccctta aagtatcagc aattattcaa caagatattt attttagcta tttcagtcat
    14101 cataaaagac taattagcaa taaaataact aatattatcc atttatcaga cgaaaagaaa
    14161 aaacttatct aattactcta tattaacttt taacaaatag ttgacttgtc caaaaatgtt
    14221 accttcaatc tcacaaagat attaagatgt ttcctaccaa aactgatatc accggacttt
    14281 ttattctgtg ttgtccaaaa gtgttacctt tagtcgatca acaaattaaa aaaaattgtt
    14341 tttttcttga cttgtccaaa agtgttaccc atactttttt aaagaagcag gttgtattaa
    14401 aattacaacc tgcttcttct caatcttctt ctaaccaatt atgcattgaa atggttggca
    14461 agtttgattc tggctcacta aaagtgaatc tgagccgatc aactcttctt cctttctttg
    14521 catagatttt ttcataatca aaagtttcaa atacctgtat tccgttttca tcaggagcta
    14581 aaaattcttc tttcgcctgc tctagtattc ttttgtcaat atttgtcatt ctataacttg
    14641 aaggtatact taatgctctt ctaaaatctt caactttgac tacccaataa ccatttcgcc
    14701 actctttatt tctgtattgc atgagatgac gatacaattc ttttgtgtag ctacttctta
    14761 gttttgtaaa attatcaagt tcaaaacgag taaagttttt gccaattgag tttaagataa
    14821 attcaaattc ctcatttgcc ttaattgtaa ctgtttcctt ctgtttatct atccggaatt
    14881 tttgaaaaag ccacagctgt tcatagacgt ctgaatcttc aaaacggtag gttaaagtac
    14941 ctagtttgtc actcaaagct tttaacacag aataaaaact gtccttttct cttctgtcgt
    15001 atttgaccaa ataacgaaag aggttgaaat caaaagttac ttctgctgtt ccttgacgtt
    15061 tcatttttga taaaatagcc caaaacacat ccatttcaac aggcttaaat cctcgcaaag
    15121 ctgttgtatt cagaccattg ttataacgca caattgcatt tgtttgttct gatactaaat
    15181 ctacatcact atcaataatc ttttcggtaa cttcttgaga cattgattac tctccaatct
    15241 tctttttgcg ctcctttaat catgttaatc tggtactaat taaaggaaac aattttttct
    15301 gcttgttacc taaattaaat cacaactaaa ggtaacagtc aacaaagatt tgttactaat
    15361 ctaattttct attttttcgc cttccaaatc aagaaaaccg aaggtaacgt cttatcctag
    15421 tgtgatacct caaatgtcca cttttgttac ctttgctgtc caaaagtgtt acccttactg
    15481 tccacttttg ttacctttac tgtccaaaag tgttaccttt ataccccttt aatacttact
    15541 ctcccaagag attaagcctc ccctaacaga tatattaaca gatatttaac agatatatta
    15601 acagatattt aataaaggag gattactcct ctcttatttc aaaatcttaa aaataaaaaa
    15661 tcttaaaaaa aactaacact atctaggagt ttattttgct tgtgtaactt tgaataattg
    15721 aaaaaaagga aaaggaggaa gcttcagctt ccccttttat ttatatttat aatatatatt
    15781 ttaatcttta aaactttagc aaccttcaaa aaggctttat atcaatctta aatagatttt
    15841 agggtggaca taaagcgatg cgaacatgga caaaaagcga tgtgaatgtg gacaaaaagc
    15901 gatgtggtat tccgaatata tccaaaaaaa gaaaaagtat gtgaatttat ttctaaaaac
    15961 tagaaaaata tttgtttttt aattttggta atttttataa tttcgtattt ttttgaaaag
    16021 gatttttata ttgaaaatgt tctaaaatag cgatttctgc tgtcttatca atgaaaattg
    16081 attaaaaaaa taaaatgtgt tttacagaga ttgagttatg ttataaattt acgatacatt
    16141 tcttattgta tcgtatcgaa aacaggtcta tacttattca atggaatcgt accagttcca
    16201 aatttttaag ctcgtaccaa gcttagaaag aagaggcaat cgtatgaaaa taacagaaaa
    16261 gaatgtaaac ccgaaatttg aacctaccga aagcaatcta aaaaagtttg atttaactgt
    16321 tactttatcg aatcaacttg tagatagcat tgttgaaatg gatgctatgc cacaaaaaat
    16381 ttatctttat gctataggac atcttgaccc taaaaatccg ccttttaata atactgttaa
    16441 agtatctaaa aaggctttat ttgcgttgat gtcttctagt tcatcttctt caaaacattc
    16501 aagattaaaa caagttattg aaaaattgca tcagcaatct gtcattaaaa ttcaaagaac
    16561 taaacctaac ggtaagactg aatacaaagt catctcaccg ctagaaatta ctgaattcaa
    16621 tgatttttct gatgcagtat ctattaaatt tagtagttca attatgcctt tattaacgaa
    16681 cttagaagat cattttacgc aatatacatt tgatcaagta gttaacttga caagcaagta
    16741 tgcgatcagt ttttttcaat tattcactaa agattttaat ctgtatgaag caaatagaga
    16801 taacttaaca tggagtacaa aatcacttga tagattaaaa aatcctgttt tctccattgc
    16861 agatttacga aagaaattta gtattgagaa aaaatataaa gcttttcgag actttgaaag
    16921 acgtgtaatt aaagaaccga ttgcccaact gaataaatac actgatttta aaattgacta
    16981 tgagcttatc aagaagggtc atacttatac aaacataaag ttcttgctta cacgtaccat
    17041 gtcagttccg gtaccttata agcaagaaca aagagaccct gcttatttaa aaggcgcaga
    17101 agataaaaca gagaaaatga atcaatcgta cttgaaagct atgaatagca aatacactaa
    17161 attactaatc aaaaactttt tgatcagttc tgccgactta tgcgatctta aacttatgag
    17221 ccgacttcag aataaagttt atccgcttta tgatcgttta tccaaagata gaggatttga
    17281 atcagttgtc aatcacctat cttacgttgc tactcataag gaggattaca caataaaaaa
    17341 tactgttaaa tatctcgaaa aatcaattac gagttatttg ccttatgtga acctttatga
    17401 ttaacaactg aaaaaatgcg tgaaggaagg aaaaactttt gaaaaaagat gaactgacaa
    17461 agtacaatga ggagttctta gcaacaattg acatgaaaat ggatttaaag tcgtatgaac
    17521 tttatcaatt gctgttcagt tatatctcac ccacaaagct aaagaaaaca ggcgtagtag
    17581 atttacctgt taaagcgatt attaattaca tggatgttga tcaaaatctt gaaacggaac
    17641 taaaaaccat agttaacact ttgcaaagac aagcagttta tgaaatttcg aatctagaca
    17701 aaaaatataa gacgtatact actgtatctg ctattgatcg gattcattgg gatttagaag
    17761 agcatagtgt atctttcagt ttttcagaag atgtaattcc cttaattgtc tcaatcaaaa
    17821 agaagtatcc gttctttgtt tttgaagact tagcatcttt tcaaaaaaaa tatagcatcg
    17881 tgttatacaa atatttggcg atgtattata aaaaatatct tatcgccaaa aataaaggag
    17941 attttgagaa agctgaaata tataagaatc ccctgattga aatagttaaa attcgtgaga
    18001 ttacgaaaac agaaaatgag tataaaacaa tatcgaattt tgatagacgt gttttaaggg
    18061 aatcagttga ggaaattaat gaaaaatcaa tgctgaatat tgattttgct aaagttagaa
    18121 aaggcagaaa ttacaatttt gctgtccagt ttaatatttc tagtgagttt gatgaaaaaa
    18181 cttatagaga aaaaaaatta ggttaatgca aaatatatct tactcgtttg aagatgcttc
    18241 agacgtatgc taagatatat agtgccttgg gagcaaggta ttaatctcct ttagaggtta
    18301 attagcttgt gatcgcctat caattttttt ggtaggcgat tctttttttt gaaacaaaaa
    18361 agcaaaaatt attttttaat tagagaggtg caatgcgatg aaatactttt tttgtgcagt
    18421 attcgaatgg aaaggaaatg aatatgcagt taaattccca gattttccag acttatatac
    18481 acatggtgaa actttagaag atgcttttga gatggcgcaa gaagtattgg aaggatactt
    18541 ggtgctttta gagttgcaag aaaaagaaat tccatcgcct acgcctgttc aagagttaaa
    18601 tgcaagaatc aagcgatctg agaatgttaa tttatttgtc gtaaaagcta atacgaaagt
    18661 tgctaaagct cgaaacaagc aaaattatga tcgaaaatca gtgactgtgg aacactattt
    18721 aaatgtttta gcggctgaag agaatattaa tctgtcgaaa gctttgaatg atttgttgag
    18781 agaaaagttg atttgatgtc tctcagatga tcatattaag caaaaacagc ttgttttagg
    18841 acg
//
LOCUS       DAJSVF010000050        18540 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSVF010000050 DAJSVF010000000
VERSION     DAJSVF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.052"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            135..782
                     /locus_tag="ON550_001748"
     CDS             135..782
                     /locus_tag="ON550_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3954833.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    911..1127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1175..3820
                     /locus_tag="ON550_001749"
     CDS             1175..3820
                     /locus_tag="ON550_001749"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR3954834.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4130..5443
                     /locus_tag="ON550_001750"
     CDS             4130..5443
                     /locus_tag="ON550_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR3954835.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5433..6086
                     /locus_tag="ON550_001751"
     CDS             5433..6086
                     /locus_tag="ON550_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3954836.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6141..6833
                     /gene="radC"
                     /locus_tag="ON550_001752"
     CDS             6141..6833
                     /gene="radC"
                     /locus_tag="ON550_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR3954837.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6983..7183
                     /locus_tag="ON550_001753"
     CDS             6983..7183
                     /locus_tag="ON550_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3954838.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7437..9116)
                     /locus_tag="ON550_001754"
     CDS             complement(7437..9116)
                     /locus_tag="ON550_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3954839.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9118..9330)
                     /locus_tag="ON550_001755"
     CDS             complement(9118..9330)
                     /locus_tag="ON550_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR3954840.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9723..11453
                     /gene="efrA"
                     /locus_tag="ON550_001756"
     CDS             9723..11453
                     /gene="efrA"
                     /locus_tag="ON550_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR3954841.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11450..13210
                     /gene="efrB"
                     /locus_tag="ON550_001757"
     CDS             11450..13210
                     /gene="efrB"
                     /locus_tag="ON550_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR3954842.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13305..13502
                     /locus_tag="ON550_001758"
     CDS             13305..13502
                     /locus_tag="ON550_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954843.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13655..14140
                     /locus_tag="ON550_001759"
     CDS             13655..14140
                     /locus_tag="ON550_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR3954844.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14244..14837)
                     /gene="clpP"
                     /locus_tag="ON550_001760"
     CDS             complement(14244..14837)
                     /gene="clpP"
                     /locus_tag="ON550_001760"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR3954845.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15017..15544
                     /locus_tag="ON550_001761"
     CDS             15017..15544
                     /locus_tag="ON550_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954846.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15635..16372
                     /locus_tag="ON550_001762"
     CDS             15635..16372
                     /locus_tag="ON550_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3954847.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16376..17293
                     /gene="miaA"
                     /locus_tag="ON550_001763"
     CDS             16376..17293
                     /gene="miaA"
                     /locus_tag="ON550_001763"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR3954848.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17295..18524
                     /gene="hflX"
                     /locus_tag="ON550_001764"
     CDS             17295..18524
                     /gene="hflX"
                     /locus_tag="ON550_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR3954849.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5992 a         3209 c         4029 g         5310 t
ORIGIN      
        1 ttattaccgc agatgcaagc ttttgaacac cgttttattt gtctcacacc cttttccttc
       61 ctacttgtaa ccttgctccc gtgttagaaa catgttacac taaatatgtg aaattattaa
      121 caaggagcgt cgttatgcga gaaaaaacag atacactgcc aaaagcaact gcgaaaagat
      181 tacctttata tttacgctac ttgaagatgc tggatgactc aggtataagt agaatcaaat
      241 caaatgagtt cagcgaaatc acccaaattc cttctgcaac gatccgcaga gacttctcgc
      301 aattaggcga gctaggcaga agcggatatg gctatgatgt cccttttctt atcgatgtat
      361 tcaataatat ccttaatact aaagaagaaa aacgaatcgc attagtagga tatggaaatc
      421 ttggcaaagc attgaaacac aacaatttta gaagaaatga aaatctaaat atcgtgtgcg
      481 tctttgataa tgatccagca ctgatcaatc gagtaattga tggagaaatg atctatccga
      541 ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac agtagcaatc tctacggtac
      601 caagtaaata ttcacaatct gctattgatg aaattgtcaa aggaaatgta accgctatac
      661 ttaattttgc gcctgacaga gtaactgtcc cagcttatgt caatgttcaa tacattgatt
      721 tgacaaccga gttgcagacg cttatttact ttgatgagaa ttactcagaa gttttttctt
      781 aggaaaattt gagtaggctt attgatcttg cgagaactcg tcgtaattct ttcaaaaatt
      841 ttttagtact aaatgaagtg gttttttcac gaccacttgt tgactaaaaa aaaaaaccat
      901 tgtagaatga agcataacgc tgaacaagag gagtaactgg cacattcatt cttagagaga
      961 gaactatctg ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg agtttctgtc
     1021 ttgaatggag tagagataga cgagtcgtga tcgttaactc acttgagagg caataatgat
     1081 tgattattgc aatttaggtg gtaccgcgta aatcatacgt cctagaatac gaaagtatct
     1141 taggacgttt tttagtttaa aggaggaaaa cagcatgacg gaagaaaata acctatcaac
     1201 aaaatataat ccgcaagaag ttgaagctgg acgttatcag cattggttag aacaagattt
     1261 gtttaaaccg agcggagata aaaaagcaaa accttattca atcgtaatcc cgcccccaaa
     1321 tgttactgga aaacttcatt tagggcatgc ttgggatacg accttgcaag atatgctgat
     1381 tcgtcaaaag agaatgcaag gatatgatac tttatggctg cctggtatgg accatgcagg
     1441 aatcgcgaca caagcaaaag tagaagcgaa gcttagagag caagggattt ctcgttatga
     1501 tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca
     1561 catccgcgaa cagtgggcta aaatgggctt atctttagat tacagtaggg aacgttttac
     1621 attagatgat ggattatctg aagcagtacg taaagttttt gttaccttat acgaaaaagg
     1681 tttgatttat cgtggtgaat atatcatcaa ctgggatcct caagcacgaa cagctctatc
     1741 tgatatcgaa gttattcata aagatatcga aggcgccttt tatcatatga gctatgaact
     1801 agcagatgga tcaggcgtag tagaaattgc gacaacccgt ccagaaacga tgttgggaga
     1861 tacggcgatt gctgttcatc cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt
     1921 gattctacct ttagtcaata aacaaatccc aatcattgca gatgaatatg tagatatgga
     1981 attcgggaca ggtgtagtaa aaatcactcc agcacatgat ccaaacgact ttgaggtagg
     2041 taatcgtcat gatcttccac gcgtaaatgt tatggatgat gatggtacga tgaatgaact
     2101 agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct
     2161 gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga
     2221 gcggacaggt gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc
     2281 gttagcgaaa aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc
     2341 aagattcaat caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg
     2401 acaattatgg tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta
     2461 tgtagggatg gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga
     2521 cacttggttt agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag
     2581 cgaagattat aagcgatact tccctactag cactttagta accggttatg acatcatttt
     2641 cttctgggta agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga
     2701 gaatgtcttg attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt
     2761 aggtaatgga atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg
     2821 gttcttatcg aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga
     2881 tgcttcttgg aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat
     2941 cgaaggaatg accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg
     3001 gatccttact cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga
     3061 atttggtgaa gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta
     3121 catcgaaatg agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg
     3181 tagcatctta gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt
     3241 cgttacagaa gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga
     3301 atatcctgtt gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt
     3361 gaaagaattg attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa
     3421 accaattacg ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa
     3481 tacaagctat atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac
     3541 tgcgccagat ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc
     3601 aggattgatc aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac
     3661 aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc
     3721 ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc
     3781 tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg
     3841 aaatcagcta gctattacta aaagctagct gattgaacat cgttacagga aagagtttga
     3901 gcctcactct tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt
     3961 ttagtctact cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata
     4021 aagtagttga taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt
     4081 tcttatgtgt tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga
     4141 agaagcaata gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg
     4201 agtcgaagct ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat
     4261 cgctggaaca aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc
     4321 tgggctgaca gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc
     4381 aatcaatggc aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc
     4441 actgatcaaa caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt
     4501 aacagcaatg gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt
     4561 tggcttaggc ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac
     4621 aacgattgga cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca
     4681 aaaagctgga atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc
     4741 ttttactgtc atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga
     4801 aaattataaa ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt
     4861 taatgaaata ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga
     4921 aaatgcagga gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt
     4981 ccgagaaaaa gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg
     5041 tttgagtaac gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg
     5101 gcttgttgag aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc
     5161 cttacgaact aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca
     5221 tatttatttg acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt
     5281 agcaagtgag agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga
     5341 aaagatgaca aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt
     5401 tcgccattta ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca
     5461 tggatggttt gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag
     5521 acaagatggg ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg
     5581 aagaagtatg ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa
     5641 gattcataga tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa
     5701 tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg
     5761 tcgcttccag taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg
     5821 aacgatttga gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa
     5881 ttttcttgtt agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag
     5941 attcacaaaa tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag
     6001 atctgcttcc accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac
     6061 atgaaatccc tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa
     6121 gatagaaagg agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt
     6181 taccaaggga acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg
     6241 caatcttact gcgtaccggt caacaccctt atagtgtgat gtcgattgct ggaaatctgt
     6301 taaaaacgtt tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga
     6361 ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac
     6421 gaattcagac ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc
     6481 agcaattgat tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg
     6541 acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag
     6601 tcgctcatcc tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt
     6661 tagcgcataa tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca
     6721 aaaggataca aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg
     6781 gacgcaaacg atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt
     6841 tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag
     6901 ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt
     6961 ttggcacgag gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa
     7021 aggatttgga tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat
     7081 ccaaggtgac ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga
     7141 atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa
     7201 attgatggta gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac
     7261 aagagacaat ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga
     7321 ggctatgaaa gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt
     7381 ttttcatttg ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat
     7441 tcttcatctg gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat
     7501 aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt
     7561 ccattcaaac attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct
     7621 cctgattctc gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct
     7681 tttttcttga tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga
     7741 cggtcacgta aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct
     7801 ccagccacaa aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca
     7861 ttttccatga tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt
     7921 actttcaaca tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga
     7981 agcattaatt tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt
     8041 ccgtgaatca cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt
     8101 gtattaccag gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt
     8161 tgacggtgag taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct
     8221 gtacatagga tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat
     8281 gtgccgtctg gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg
     8341 cttcgtccga aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc
     8401 aaacggaaga tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag
     8461 atcttcaaaa tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca
     8521 tttgtactat cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc
     8581 aagtttgctg gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata
     8641 tttccagaag gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg
     8701 aaaaagctga cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc
     8761 tctgcatctc gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga
     8821 ggaccagcat agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg
     8881 tcttcgtgcc cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag
     8941 ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg
     9001 gcatcaatca aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca
     9061 cctaatccac caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta
     9121 aaactccact aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag
     9181 aagttgaacg aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac
     9241 atcgcttttt gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt
     9301 tgtttcttga taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag
     9361 tgatttagct agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt
     9421 tcatatgctt tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta
     9481 tctgttcgga tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt
     9541 tattctagca taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa
     9601 tgtttatcat cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag
     9661 gctcgttgtg ctatcatttt tagtaatgaa aatgtaacga aagattaaaa ggagatgaga
     9721 aagtgaaact aatgtggcat tataccatgc gttataagaa attcttgttt ttcaatttca
     9781 tatgcgtatt tggttttatt ttgattgaat taggacttcc tacgatttta gcacgtatga
     9841 tcgatgtagg aatccgcaat gatgattttg attatgtgaa gcaacaaggg atatttatga
     9901 tcgttattac tgtaatcggt attattttga atatcttgtt aggttatttt ggttcgagga
     9961 ttacgactaa tatcgtagca gatattcgag atgatttgtt cgaaaagatc caaagttatt
    10021 cgcatcagga atacgaaaat ctaggcgtat cttcattgat cactcgtaca acaaacgatg
    10081 cttatcagat catgttgttc ctgcaaaata ttttacgtat cggttttatg gcaccaatga
    10141 tgttcattgt cagtttgtac atggtcatgc gtacaagtgt gacactcagt ttgtatgtag
    10201 taggtgcatt acctttacta ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt
    10261 tatctaaaaa gcaacaaaaa aacttagata aaatcaacag tatcttgcgg gaaaatcttt
    10321 caggattgcg tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg
    10381 tagtcaatga gaattatgct tctagttcaa aaagcttatt ccgtttgatg gctgttgcac
    10441 aaccgggttt ttatttctta tttaatatcg taatggtatt gatcatctgg agcggggcag
    10501 tgcaaatcga tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc gagtatatat
    10561 tccatgcctt gttctcattc atgttatttg ctagtgtgtt catgatgtat ccaagagcag
    10621 ctgtttcagc tggacgtatc caagaagccc tagatgccaa accttcgatt gtagaagatc
    10681 caaatggtat atctgataca aaaactaaag ggtatatcga atttcaaaat gtaacatttg
    10741 cttatccagg ccattcgcaa gagccagtga tacgaaatgt cagttttaca gcctctccag
    10801 gagagaccgt tgcttttatt ggaagtaccg gtagcggaaa atctacgttg atccaattga
    10861 tccctcgctt ttatgatgtg agtgaaggaa agatcttgat cgatggagta gacgtgagag
    10921 attatcggct aagcaagttg cgagataaga ttgggtttat tccgcaaaaa gcgctgctat
    10981 ttactggaac gattgcagat aatttgcgat acgggaaaga agatgctacg caagaagaaa
    11041 tggaacgtgc agctgagatt gctcaagctt cagaattcat ttctcgaaaa ccagatggat
    11101 ttaatgaaca tttatcagaa ggtggttcca atttttcagg tggacaaaaa caacgattgg
    11161 caatagccag agccatcata cgccgtccgg aaatctatat ttttgatgac agcttttctg
    11221 cattagatta tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat
    11281 ctactgtatt gatcgtagca caacgtgtgg gaacgatcat gcatgcagat aagatcatcg
    11341 tattaaatga aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc
    11401 cgatttatta tgatattgcc gcttctcaat tatcaaagga ggaattagca tgaaaaaaac
    11461 cttttcttcc ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga cttttctttt
    11521 agtgatccta tttacagttc tgacagtggc tttcaacgtg gctttgccat atgttacagg
    11581 acttcctact acagaaatca gccgtaatat tgccaatggc gaatcaatca atttttctta
    11641 tatattcaaa tgtctcattt ggatcacagt tgttggaata gggtattgta tttcgcaatt
    11701 actttctggt gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga
    11761 catagaagaa aaaatcaatc gtcttccggt ttcatatttc gataaaaacc agcaaggaaa
    11821 tattttgtct cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac aacaagcttt
    11881 tattggtatc gtaaatgcga tattgggaat tacgttggct gcagcaatga tgttttatat
    11941 ccagccgatg atggcgctga tctctatgat catgattcca acatcgatct ggatctcaaa
    12001 acgagtgatc aatgcttcgc agaaatattt tcaaagcatg cagaattctc ttggtgagct
    12061 aaacggctac gtccaagaaa atatgacagg ttttagtgta ttgaaagtat atggacgcga
    12121 aaaagaaaca ttggaaggat tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg
    12181 tgcagcattc atttctggct tgatgatgcc tctagttcaa ttaactgcct atgctactta
    12241 tattggtatg gctgttctag gaagctttta tgttattact ggggcgatcg ttgtcggtca
    12301 actacaagca ttcatccaat atatttggca aatcagtcag ccaatgggga acgtgacaca
    12361 attgtcttca gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga
    12421 tgagccagaa gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga ttttaggaga
    12481 tgtgacattc gatcatgtcg attttgcata tgatccgaag aaaccattaa tccaagattt
    12541 gaattttgaa gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa
    12601 gacgacattg atcaatctat tgatgcgttt ttatgacgta gacaaaggag caataaggat
    12661 tgatggaatc gacaccaaat caatgagtcg cagtgatgtg cgttcgctat ttggtatggt
    12721 cttgcaagat gcatggcttt atcaaggaac gattgcagat aatatccgct tcggaaagct
    12781 tgatgcgaca gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag atcattttat
    12841 ccgaacgatg ccagatggat atgatatggc catcaattct gagggagaca atgtgtcttt
    12901 aggacaaaaa cagttgctga cgattgcccg agcagtggtc tccgatccta aaatcttgat
    12961 tttagatgaa gcaacaagtt cagtagacac ccgtttagaa gcgttgattc aaaaagcaat
    13021 ggatcgcgtg atggagggac ggacaagttt cgtcatcgct caccgtttat cgacaatccg
    13081 tgatgcagat ctgattctag tgatggatca aggtcagatc attgaaaaag gaacgcatga
    13141 gagtttattg gcacaaggtg gattttatga aaaactgtat aacagccaat ttgctgagga
    13201 agcagaataa gtcatataag aataaaacag tttaaaactt attttttgtt ctctattctt
    13261 gataatggta aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa cagtttttaa
    13321 atgaaaaaat tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga
    13381 agccttttga ttcagctgat ttttttgaaa atgagtttca aatcgaacgc ttggcagcac
    13441 aaatagaagt gtacgaatta attttaaaaa aagtaataga agaaaatata gatgtacaat
    13501 aaaaacaggt aaaacgtcag ttaccagata ctgtaaatag acatctggcc tgagcaggtt
    13561 ttacctgttt tttttcttat gaaataaata tatgctatga tacctttatt caataggatt
    13621 agtttctaca aaaaatgaaa ggagaaaaat aaatatggaa cggtttatag catcaaatgc
    13681 cacagtgatt ggtgatgtga cattgtcaga agatgtgacg atctggtatc aagctgtttt
    13741 acgcggggac agtaattgga taaaaatagg acaaagaacg aatatccaag atggtacaat
    13801 catccatgtc gatcatgatg cacctgtaga tattgcggag aatgtgacgg taggacatca
    13861 gtgcatgcta catggctgta caatcgaaaa aggggcgctg atcgggatgg ggaccacgat
    13921 attgaatcat gcggttattg gtgagaatag tttgattggt gctggatcat tagtaacaga
    13981 aggaaaagtt attcctccta atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc
    14041 tttgacaaaa gaagaaatac aaaaaaacaa agaaaacatc cagcattatg tcgagatagg
    14101 tcggatgcat gcgcaaggaa aattcaagga gcaaagatag gcagtagcat ccataaaata
    14161 aaaaaggatg gtcagtttga gttgaccatc cttttttatt tggtttagat aagagagctt
    14221 aaaaaagctt cctttgaaac agattaattt aatgaagaac tgttttccat gacttcatcg
    14281 atcaatccat atgcttttgc ttgttcagct gtcatatagt tgtcacgatc tgtatctttt
    14341 tcaatcactt caagcggttg tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt
    14401 gtttgcaaaa tatggcgtgc cgcaatttcg atttcagttg cttgcccttg agcgccgcca
    14461 agaggttgat ggatcatgat ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca
    14521 gctgtcagca agaagcttcc cattgaagca gccataccca taacaattgt ttggacatct
    14581 gctttgacaa agttcattgt gtcgtaaata gccatacctg ctgtaactga accaccagga
    14641 gaattgatat aaagatagat atctttttca gaatcttgag cgtctaagaa aagcaattgg
    14701 gcgatgatcg aattagcaag atcatctgtt acttggccgc ttaacatgat gatacggtct
    14761 ttcaataaac gtgagtagat atcgtaagct ctttcacctc gtgaagattg ttcaatgact
    14821 gtaggaatta aattcataat agagttcctc cttgatttcg tttagaaata gtgtaccata
    14881 atttagtttc taatacctaa aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa
    14941 aacttaggca aattagtcgc ataaacccgc tttatggtat gataagcaga gaaacaaaac
    15001 aaggaggaac aacagattga tagttggtat tactggcctt atcggagtgc tgacaggtgc
    15061 tgtagttgtc tatatcggca tgatactgca aatgcgtcac ttggaaaata aagaacagcg
    15121 tagacgtgaa ttggaattga aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa
    15181 gatcaacgag atcttgcaaa aacggacaac gctgatggaa gaatatgtca gctttgatgc
    15241 atttgatgat tgttatatca cgattgacga ttttgcgtat ctccaatcat tttcagcgca
    15301 aaatagtttt tatctgccaa attatatttt agacgagcta tttaagaata tctcccatcg
    15361 aaaagtgatt ttatctccag atgaaacaat gaagattggt ggatatgctt ataaaggcgg
    15421 acgcgtgatt ttagagaatt ttacggatag tctgactgaa atgatcaatg aaaaaaaagc
    15481 tcagttgaag actatgacgc gccagccgat tggtttcttc tcgaaagatg atgtttggag
    15541 ctgatatttt gaagcattgc cgttgcacat gagagcgtgg taatgctttc tttttttctt
    15601 aatgatacaa tagaactgaa cggagtgaag aaagatgtta caaatctatg cccatcgcgg
    15661 aagcagcggc acccacccag aaaatacgct tcctgcattt gcagaagctg tacgagtagg
    15721 tgcagatggt atcgaactag atgtccatct aagcaaagat ggttatttga tcgttatgca
    15781 cgacgaagaa gtggaccgga cgactaacgg taaagggctg attcgagaaa aaacattgga
    15841 agaactcaaa aaattgaatg cagggggcaa attcagcaaa gaatttccag cagctaaagt
    15901 tcctgaattg agagaagtgt taggactgct cctccaaaaa aattaccgag ggatgctaat
    15961 gattgaaatt aaaactgatc agtatgaata tcccggtatc gaagaaaaat tagccgaaac
    16021 attaaaaagt agagattggc cttttcaaca tgcatattgc agctttcatt tggattcttt
    16081 ggaaaaaatc gcattactgg aaccggagat acagctggac ttattgatga gtacttctga
    16141 taaaaagatc caactagcag cagaaacccc ttatatagaa ggagtacatc caaaaaatac
    16201 ttggttgttc gaacatccag tccaggcagc tgattttcca aaagcaattc gtgcatggac
    16261 gattaacgat gaagaagaaa tggccaaatg tcaagaactc aaaatagatg gaataataac
    16321 tgattttccg gagaaagctc ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga
    16381 aaaagtatta gtgatcgtcg gtccgacagc agtcggtaaa acagcgctaa gtgtggaact
    16441 cgcacaacta tttggaggag agatcatcag tggtgattca ttgcaaatct ataaaaatct
    16501 ggatatcggt acagcaaaaa tcaaagaaga agagatgaat aatgttccgc atcatttgat
    16561 tgatgtgatc gaacctactc aacgttactc agccgctgat tttcagcatt caggtcgaca
    16621 attgattacc gagattgcga accgagggca cctacctatc atagctggtg gtacgggtct
    16681 ctatatccaa tcccttctat atgattatca gttaggagcg acagaagaag cgaatcctga
    16741 gattagaaaa aagtatgaaa cgttagctga aacaattggt tcccaagcat tatggaatca
    16801 gttgaaagaa aaagacccgg cagctgctga gaaaatccac tggaacaatc aacgcaaagt
    16861 gataagagct ctggaagttt tagagacgac aggacatagt atcacggcac ctaaagaaga
    16921 gccaaagcaa ttgtatgatt acctcatgat tggattagat acgaagcgta gtattcttta
    16981 tgaacgaatc aatcaaagag tcgatttgat gctagcagaa ggtttagtag cagaagcgcg
    17041 taaggtgtat gagcttggag aagtccaggc cagtcaaggt attggatata aagaattttt
    17101 cccttatttt gaagggaaag aaacattgga agaagcaaca gaacaagtca aactacattc
    17161 gagaagatac gcgaaacgtc agttgacgtg gtttcgtaac cgtttggatg cacagtggtt
    17221 tgatcttata agtgagccaa atcaaaaaag tcagattgag caaacaatca aaaaatggct
    17281 ggaggcggaa taaaatggaa gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc
    17341 agcgttactt ttcagagtca atggaagagc tagttcagct gacgaatacc gcatcaggag
    17401 aagtcgtatt cacaattaca caaaagagac cacaagtgga tcggcaaacg attatcggta
    17461 aagggaaatt acaagaatta gttcaacaag cggatgcgca tgaagcggat ttgatcatct
    17521 ttaatcatga gctaactccg agacagagcc aactgattac cgatgctgtt ggtcttccgg
    17581 taattgatcg cgtgcagctt atcttagata tttttgcgat gcgtgctcgt tcgaaagaag
    17641 ggaagctgca ggttgaactt gctcagttag aatacctgct gcctcgttta gtagggcaag
    17701 ggaaaacatt atctcgtctt ggcggtggga tcggaacaag agggccaggt gaaacaaagt
    17761 tagaaacaga ccgccgacat atacggaata aaatttttgc tgttaagaaa gaattgaaag
    17821 aagtagaagc tcatcgagag cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga
    17881 ttggacttat tggctacacg aatgccggga agtcaactat cttgaatcta ttgacacaag
    17941 cggatactta ttcgaaagat caattgtttg caacgttaga tccattgact aagagatggc
    18001 gttttgctga aggatttgag attactgtaa cggataccgt cgggttcatc caagacctgc
    18061 ctacgcaact gatcgatgct ttccattcta cattagaaga aagtcaaaat atggatcttt
    18121 tgctacatgt agtcgatgct agttctccag atcgtatttt acaggagcag actgtcttaa
    18181 aattgatgga tgaactgaat atgaaagaaa tgccgatact gactgtttac aataaggcag
    18241 accagatcga cccagcgatg ttcacgccta cgctattccc aaatgtattg atttctactc
    18301 agacgaaaga aggtaagatg gcgttgatcg aagcaatcaa acgtcagctg atggaactaa
    18361 tgacccctta tacactttct gtgccaagca acgaaggaca gaaattaagc gagttgagaa
    18421 gacaaacaat ggttttggac gaagcgtaca tggaagaaac aaacgaatat gaagttaaag
    18481 ggtttgcaaa aaccaattca aaatggttaa gaaaaaacca ataaaatttt atttttttaa
//
LOCUS       DAJSVF010000051        17405 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSVF010000051 DAJSVF010000000
VERSION     DAJSVF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.053"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(83..1378)
                     /gene="purB"
                     /locus_tag="ON550_001765"
     CDS             complement(83..1378)
                     /gene="purB"
                     /locus_tag="ON550_001765"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR3954850.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1391..2515)
                     /gene="purK"
                     /locus_tag="ON550_001766"
     CDS             complement(1391..2515)
                     /gene="purK"
                     /locus_tag="ON550_001766"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR3954851.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2508..2996)
                     /gene="purE"
                     /locus_tag="ON550_001767"
     CDS             complement(2508..2996)
                     /gene="purE"
                     /locus_tag="ON550_001767"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR3954852.1"
                     /translation="MAAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3412..4719)
                     /locus_tag="ON550_001768"
     CDS             complement(3412..4719)
                     /locus_tag="ON550_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR3954853.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4722..5303)
                     /locus_tag="ON550_001769"
     CDS             complement(4722..5303)
                     /locus_tag="ON550_001769"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3954854.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5419..5516)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5875..7167)
                     /locus_tag="ON550_001770"
     CDS             complement(5875..7167)
                     /locus_tag="ON550_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3954855.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7164..7787)
                     /locus_tag="ON550_001771"
     CDS             complement(7164..7787)
                     /locus_tag="ON550_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954856.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7790..8626)
                     /locus_tag="ON550_001772"
     CDS             complement(7790..8626)
                     /locus_tag="ON550_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3954857.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8643..9530)
                     /locus_tag="ON550_001773"
     CDS             complement(8643..9530)
                     /locus_tag="ON550_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3954858.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9546..10829)
                     /locus_tag="ON550_001774"
     CDS             complement(9546..10829)
                     /locus_tag="ON550_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3954859.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11512..12195)
                     /locus_tag="ON550_001775"
     CDS             complement(11512..12195)
                     /locus_tag="ON550_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3954860.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12213..12830)
                     /locus_tag="ON550_001776"
     CDS             complement(12213..12830)
                     /locus_tag="ON550_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3954861.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12840..13664)
                     /locus_tag="ON550_001777"
     CDS             complement(12840..13664)
                     /locus_tag="ON550_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR3954862.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13657..14217)
                     /locus_tag="ON550_001778"
     CDS             complement(13657..14217)
                     /locus_tag="ON550_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954863.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14391..15347
                     /locus_tag="ON550_001779"
     CDS             14391..15347
                     /locus_tag="ON550_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3954864.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15652..16608)
                     /locus_tag="ON550_001780"
     CDS             complement(15652..16608)
                     /locus_tag="ON550_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR3954865.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16712..17314
                     /locus_tag="ON550_001781"
     CDS             16712..17314
                     /locus_tag="ON550_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3954866.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
BASE COUNT         5127 a         3816 c         3011 g         5451 t
ORIGIN      
        1 aagcagaacc gctaacggat ctgcttcttt tttggttatt tttgctattc tattttaatt
       61 gaattttgca actcactctt ctttattcca aacctactcg tttgaatatc acgtcaacgt
      121 ttttcaaatg atgatgataa tcaaaggcat cgtctatttc ttctttagat aataccgatg
      181 tgatggttgg atcttcttct aacagcgggc ggaactgcac ttggttgtcc caagcgtacg
      241 cagtttttgg ctgaacaaga tcataagctt cttctcttgt cattccctta tcgatcaatt
      301 tcagcataac gcgctggcta taaatcaaac caaatgttgc agacatattc cgcttcatgt
      361 tttcaggaaa gaccgttaga tttttaacga tattgccaaa acgatttaac atgtaattca
      421 ataaaatcgt ggtatcaggc aagatgattc gttctgctga agaatgagaa atatctcttt
      481 catgccataa tgtgacattt tcataggcag tgaccatgtg tccgcgcacc actctggcta
      541 gaccagccat attttctgaa ccgatcggat tccttttgtg cggcatagca gaagaacctt
      601 tttgaccttt cgcaaaaaat tcttctactt cacgtgtttc ggatttttgc aatccgcgta
      661 tctctgttgc aaaacgttca atacttgtag caataagcgc catcgctgag aaatattcag
      721 catgtagatc ccgagggaga acttgagtag agatttcttg tggacggatc cccaattgtt
      781 tacatacata ctcttcaata aatggcggga tatttgcgaa cgttcccact gctcctgaga
      841 tttttcctgc ttctactcct tttgccgcat gttcaaatcg ttcgatgttc cgtttcattt
      901 ctgaatacca tgtggcaagt ttcaatccaa aagtagttgg ttcagcatgg acaccgtgtg
      961 tccgacccat catcactgta tatttgtgtt cttttgcttt ttcgccaatg atttccgtaa
     1021 aacgtttcaa gtcttcccgt aaaatatcat ttgcttgctt gatgagatag ccataagcag
     1081 tatctacgac atccgtactt gttagaccat aatggaccca ttttctttct tctcccaatg
     1141 tttcagaaac cgctcgtgta aacgcaacga catcatggcg tgttaatttt tcgatttcca
     1201 agatccgatc gatatcaaat gatgcatgct cacgaatttt tttcacatct tcttgcggga
     1261 tttcaccaag ttctgcccaa gcttcatctg ctaaaatctc gacttcgagc cacgcacggt
     1321 atttgttctc ttccgtccag atattcccca tttcagggcg tgtataacgt tctaacatgt
     1381 tttttctcct ctaatcccaa atattcgttt gtttgatttc ttccagcgtt tgctggatgt
     1441 cttctgtcaa gatagtgata tgtcccatct tgcgaccttt ttttgcttgt ttttttccat
     1501 agaaatggaa ttgccagtct ggcttattct caatcaagcg atagctttcc agcagttgtt
     1561 ctcccaaaac attgaccatt actgcattgc ttatcaaacg tactttttct cctaatggcc
     1621 aaccacaaat acctcgaata tgggcatcga actgactcat agaacaagct tcaatagaat
     1681 aatgtccaga attgtgagga cgaggagcta attcattcac atatagcccg cctgtttttg
     1741 ttaaaaacat ttctacccct aaaacacctg aaaggctcac tgcttctgca atcacacggg
     1801 caatacgctc agcctcctcg atcacatcct gatcaatcgc ggctggagca atcgtttcat
     1861 gaagaatatt gttgtggtgg atgttttcca cgaccggaaa agtcgtgaaa tctccttgac
     1921 catttcctgc caccatgatc gaaatctctt tttcaaacgg gatccatgct tctaaaacac
     1981 aggttccttc tcttaaaagg tccatggcag gagctagatc cgcccgactt ttcaaaacat
     2041 actgcccctt gccatcatag ccgcctcgag tggttttcaa aacacaagga tagccgatcc
     2101 cgtcaatcgc atcttggata tccgtcggac tgacgatcgt tgcataagga gcaatcacga
     2161 tgttattcgt ttccaaaaaa gatttttcta acaggcggtc ttgcgtaatc gcaagcaagt
     2221 ctgtcccttg aggaataaat gacatgggta aaatcgcatt taacgcctct acactgacgt
     2281 tttcgaattc ataggtaatg acatctgttc ttctggcaag ctctttcagc gcaaaggtgt
     2341 catcatagac actttcaatg tgccaatctg caacttgtgc ggctggacaa tcgctcacag
     2401 gatccagcac acctacttta aagcccattt cttttgcact aatcgccatc atacgaccta
     2461 attgaccgcc accgacgatc ccgatcgttg aaccaggcaa caaaggttta accaagttga
     2521 tcactacttt ctataacagt tttagccaac attgaacgtc tttcagccaa tttagcctcc
     2581 aattctaaat catacatgga aagcatctgt atcgcaagta gtccagcatt aattgctccc
     2641 gcttttccga ttgctgtagt agctacaggt actcctccag gcatttggac aatcgaaaga
     2701 agcgaatcca aaccatttaa tgttcgtgat tggaccggta caccgataac cggcaaagtt
     2761 gtttttgatg cgaccatacc cggcagatgc gcggctcccc ctgctcctgc aataatgatt
     2821 ttgattcctt tttttcgagc gttttgtgca tattcaaaca tcaaatctgg ggttcgatga
     2881 gcagaaacga cctttttttc ataagacaca ccgaattcat ccaatatttg acatgcttct
     2941 ttcattgttt cccaatctga agtactcccc ataatcacag agatttttgc tgccattgtt
     3001 tttcctccca gtttttctac attcatattt taacaatcac tttttttgat gtcaaagaaa
     3061 tttcgaatgt taacaaagaa aaacatttta ttgttcgtgt ttattaaaga taaaaaaaag
     3121 gaatgtgaca aaagttaggt cacattcctt tttatggata aacggtgttc tcctgccaac
     3181 tcctcggtca caaggaacaa tattcaacaa acatgagaag cttttttttg aaattgttcc
     3241 ttgtgactcg gagatgaacg gcaggctcac gacctcttcc ttcaacggtg ttcaatcagc
     3301 tgaccctcgg tattagctca aacaaacggc aaaatacgag gaactatttt tccgtttgtt
     3361 tctctaacaa tcaggtcaaa acgctgattt cacaaccttt cttttgctct attacttctt
     3421 gaaggcttct tccaatattt cttcatcttt tttcaatcca ttaaagatca aattcaatat
     3481 gatagccgta atgctactca ttacgatgcc atttccagtg aacatttgca atgtttgcgg
     3541 catttgactg aataatgtcg gcgtaacatt gaaacccagc ccaaatccaa cagaaacagc
     3601 aacaatcaat agatttttat cattgctgaa atcaacttcc aacagcatac ggattccttg
     3661 gactgccacc atcccgaaca tgactaacat tcctccacca agtaccggct cagggatgat
     3721 ttgtgctaac gcaccgattt tagggaataa tcctaaaaca atcaagaaga atgcagagaa
     3781 atagatgggt tttcttgttt tgattcccga aagttgaacc aatccgacat tttgagaaaa
     3841 gccagtataa gggaatgtat tgaaaatacc gcctaagata acggcaagtc cttctgcacg
     3901 atatcccttt ttcaattctt cttctccaac atttttccca gtaatgtcac caagtgcaaa
     3961 atatacacct gttgattcta ccatgctgac catggaaatg atgatcatta gtaaaatcga
     4021 ccatacgtcg aaagttggtt taccaaaata aaagggttgt ggaacatgga acaacggcgc
     4081 atctccaatc gcgctcagat caatcattcc catgaaagca gctaagatgc ttcctccgac
     4141 taaaccaatc aataccgcga ttgaacgaat gaaccctttt gccagcattt gcacgcctac
     4201 aattaggaga atcgtcacaa atgcaagtag cagatttgtt ttgtcaccaa aatcagctgc
     4261 tgttgcatta cctccaccca ttttctcgat tgctacagga atcaaagtca aaccaataac
     4321 ggtgataacc gttcctgtta ctataggagg gaatagtttt ttaattttag aaaagacacc
     4381 agaaactaac acaacaaaga tcccagaagc gataatagat ccgtagatag ctcctactcc
     4441 ttcattggtt ccgatcaaga tcagtggagc tactgcttgg atcgcacatc ctaaaacgac
     4501 aggcaagcca attccgaaaa atcggttaac tgtcaattgc aataatgtcg ctaatccaca
     4561 cataaagata tcaattgaaa tcaaataagt catctgttct tgattaaagc ctagtcctgt
     4621 accgatcaaa agaggcaccg ctactgctcc tgcatacatc gctagcagat gctgcagccc
     4681 taaaaccgca gcttttccat tttgtgtttc tttttccacg attatgcgtc ctcctcaaga
     4741 aactcaactt gtccattcgt caaggaagca atacgcgcaa gagaaacgac tctcaatccc
     4801 atttcttcaa gtaactgacg tccatcttgg aaagactttt cgatgacgat cccgattcct
     4861 tctacttgcg ctccagcttg ctggcataat tcgatcaatc cttttgccgc ttgtccgttt
     4921 gccaagaaat cgtcgataat caatactttg tcttctgaag ataaaaattt acgagaaata
     4981 gaaatcgtac ttgtcacttg tttcgtaaaa gaataaacag aagctgtcaa taattcttca
     5041 tccatcgtca aactttttgc ttttctagcg aagatcattg gaacatctaa ttctcttgca
     5101 gcaaataaag ctggtgcgat cccagaagct tcaatggtca caactttcgt gattccttgt
     5161 tctttaaata cttctgcaaa ccgttttccc atttgttcca tcaatacagg gtctacttgg
     5221 tgagtgacga agctgtcgac ttttaatacg ccctcgccta atatacgtcc gtctttttga
     5281 atacgttcaa ctaattcttt cactttattt gctcctttat catttattaa taccgaaata
     5341 accgcttttg tgcaaaaacc tactatgttt ctgttaaaga aaaacaccct ccactttatc
     5401 ataagaaaag aagggtgaaa tagccaatct tttacttata gtccagcatt tacggtactg
     5461 gctagaaact gtcatccata ttatgacgat atataagtag gtatgtaaaa ctaataatag
     5521 tgtaaattca ttttcttgca actgttttgc acgtgaaggc agtttgccat atttgaataa
     5581 aaaatgttgt ttcaaatatt gaaaacagtc taacactact ttctgaaact gacaacgtgg
     5641 tttacttatt tttttgtaaa atctaatgat ttttaatgca ttattccttt ttgttcgggt
     5701 ttaaatagtt atttttattt tctcctatct aaaaaagcag cccacataaa aagaaggcat
     5761 gacaaaaagc atgtcatagc ccctttttcc gaataaatag tgttcaaaag acagcctatc
     5821 ggcaataagg caagatttta taaactacgg gaagtaattt ccgtaaatct attcttattc
     5881 ttttaagctg acaagctcgt caatcactac tgcttttcct gaagcaatag aatgatttgc
     5941 tgcgattcct gtcaaaatcg acaaaatacc atctacgtga gttgctgccc gattccacgg
     6001 atcttcttct ttttcttcac caaatagatc atctaataaa cgcggatctc ctccgccatg
     6061 tccgcccgct tcttgttcga cgattacctc atatggctca ccgaacatcg gatagacatg
     6121 gatggactga agaacggctg ctccttcatc ctttttatct ccacctgcat tgatgtaaga
     6181 cttttcgatc actttcattt cgatccgtcc tttgctgcca ttgaaagaaa cattgaatcc
     6241 ttcccacggc atatacgcgt ttagcgaata cgtcaaaatc gcaccagttt catatttgac
     6301 cgttacgcct agtgtatctt caatagagat atcatcggaa aatacactgc ggtcacgaat
     6361 atatccactg tctttttctg cttctaaata catttctcgc aattcctcat tggctgacag
     6421 gtctatagca aatggatcag ctttagctag ttcgcttcca tagcatcgac tgtaaaatat
     6481 ttctactccg cgtttctccg cattatgcat cccataaaag tttaatccac cgaatgcaaa
     6541 taccttttgg ggacggctgt tcaaccaaaa attcactaga tcaaaatgat gggtagattt
     6601 atgaacaaga agcccgccgc tattgtgctt atttcgatgc catcttctga aataatctgc
     6661 cccgtgttgc gtatttaaca gccattcgaa gtggatagaa aacacatcac caatcacatc
     6721 actttctatc agttccttta tcttcgtgtg atacggtgca taacgataat taaaagtgac
     6781 tcgcaggctt cttcccgtct gtttttggac cgttattatt tcttgagctt tttcctcatc
     6841 gatagtcaga ggtttttcgc aaataacatc atatcctttt ttcattgcac gaatgatata
     6901 atcatgatgc gttcgatcta ctgacgtaat aatgacttca gccggccgta cgtcatccag
     6961 tagttgctca aattgtgatg gcatacatgt ctttactttt ttctgtccat acttgttcac
     7021 aagtatccga tttgcataat ccatccttgt ttgagataca tcacaaaaac cgactagttc
     7081 acaagtttga tgatacgtag aggcaattgc ctcataaaaa aaacgcgctc tcccaccaag
     7141 tccgacttgt acatattttt tcttcattta aagtcattct ccttttcttt taagcaaaat
     7201 actgtgacaa atggcagccc tggatacagt aatgcagtga gtaaaaaatc atgctgtata
     7261 caccagaaca gtccatacaa aaggcataaa taccacagaa accccttgac gttttttgac
     7321 aacagccaga tattcctttt gatcaaataa acaagactat ctgctttatc ttctttgcag
     7381 atatcaatta gcgtactaaa aaaactatgg gatacaaaaa gcaacagaca caatagaaaa
     7441 ggaatcagat accaagctcc aatctttccc actaataaat accaatcaac tgcaaaaata
     7501 atgacaaaca gctgaaacaa cgtaatgatg aaacacatcc gtttgatttt tcctttcctc
     7561 caacatacct ttttttctgc caagtaatgg acagatagat aataagaagg atacatcatc
     7621 aaaaaataaa tcagatagac aacacgagca gacagcacta atcctaaagt gaacaaacat
     7681 cctagaacta aagcgaaaag agcattactg ataagactgg ttaaaaaaat ccgacagaca
     7741 tagtagttga gcttgtaaag aaacatctct tctattggct ttttcatatt cagcccttca
     7801 gaccgcttgt actgatcccc tggacaagat atttattgaa gaataagaaa atcagaaaaa
     7861 ccggtagcag cgacaacgta gacatcgcaa acatactcgc gtaagtcgta ctagatccag
     7921 cgtctgcaaa taatttgatg gcaatcgata ccgtgtaaga tctcggcttg tttaaataaa
     7981 tcaatggtcc catgaagtcg tcccatttcc aataaaactg gatgatgatc gtcgtgatga
     8041 tcgaaggttt cagcaaagga aaaataattt tggtaaagac actatacttg ctgcatccgt
     8101 caataatcgc tgattcatct aactctttgg ggatgctgac catgaattgc atgatctggt
     8161 aaatgaaaaa tgcttgtcca aaaaaatgtg gtaaaatcag cggcacatac gtcccaacca
     8221 aattcagccg attgtaaata ataaactgcg gaatcaaaat cacttgtgca ggaatcatca
     8281 tcgttactaa catgacgacg aaccagaatc tttttccttt gaaatcgatt ctcgataatg
     8341 cgtaagcaat acaagtcgag ctcagaactg tccctatcgt cgaaactgca gagatgacaa
     8401 acgaattcct aaagaaagta aaaaatgaaa ttccagcaaa ccccgcccat ccttttgcgt
     8461 aattttccca ttgaaaagtc tctggaatca aattcagcga accactcata atctcttgat
     8521 ccgttttaaa agaacctgcg atcatccata agaccggata aatcatgact agacctaata
     8581 gcaaaataaa accgtattga aaccatttcg ttactgtatt attcatttct tcctcctctc
     8641 attcacacaa cgacccattt ttttgatgat ttgaaaatca gaaaggtgac agctcccata
     8701 atcaacagca tgatccacga aatagcacta gcatacccca tagaattaaa agaaaaagct
     8761 tggttataca cataaagagc ataaaagttc gtggcatcat tcggaccacc ttgggtaatg
     8821 ataaatgcct gcgtaaaagt cataaacgcc gcgattgtct gcataaccaa gttatacaaa
     8881 atgatcggcg aaagaagagg taacgtgatc ttgtaaaacg tctgccattt gttcgctcca
     8941 tcgatctctg ccgcttcata atacgagcct gggatctctt tgatacctgc agcaaagatg
     9001 atcattgccg aaccgaattg ccaagcagac atcaaaacaa gagggatcat gaccagtttt
     9061 tgatcgctaa accaggaaag cgcaggtgcg cctaatcgtt ccaggaaaat attcactaac
     9121 ccgtttttag aaaacaactc ccgccaaact agagcgatag aaatactgcc accgatcaaa
     9181 ctaggcacat aatacaaaga acgaaagaaa gtcgttcctt tgatattcct cgttaaaaga
     9241 taagcaatca atagaccaaa gatcagtttc acaggtacag atgcaaatac atagagaaaa
     9301 gtgactcgga tggagttcaa aaaacgggca tcactcagca tctttatata attatccaag
     9361 ccgatccatg agactggatt tcccaaacga taatcggtga acgaataata aagtgacaag
     9421 atcatcggta tcactgttat caatagaaat cctaggatga atggagaagc aaatacgtaa
     9481 ccgaccacat tgtcttgatc caagaaccgt tttagttttt cttttttcat gccttcctcc
     9541 tgctgctatc ctagaatgtt tgacgcaaac tcataaagac tctctgctgc ttcttctgct
     9601 gttacttctt cataaatgac tttttcaact aaaagattgt attggtcttg aatttccgga
     9661 ttcaaagcac tatcaaagac attcacttcg ccgttgtcca tatcggaaat catttctaaa
     9721 taatcaaatg tcgcttgcaa agttgcatca tctgcacgag cgagctgttc tcgaatattc
     9781 gacatgattg gtacaccacg ttctcctttt aaaatttcat tggcttcttc actgttttga
     9841 aagaaatcaa taaacttagc agcttcttct ttatgggctg agctttttgc catactcagc
     9901 atttgcgaag aacgtaaagg gataccagat tctccatctg cttttctgcg aggtggatta
     9961 atcaactgaa tctccatatc ttcaggagcc cctgacaata tttctgtcaa ctggttactt
    10021 gatagaaaca ccattgccgc ttctctatcc ctaaccgcct gaaaagacag atccgtcgta
    10081 cttgcagctc caccacctgg ttctggatgg gcccctttgt ctaccagatt tttacgcagc
    10141 tggaaaaaat ctgttagata gtctggattt tcaaatccca agctgttaga tagatcttcc
    10201 cgattataaa aattcaatcc tttctcaact tgtggtattt gaataataca gccagctata
    10261 aaatcttcga atcttgaaat agcatagatt cctaattctt cagtgatttg atagctgatt
    10321 tgttcaaatt catcccatgt ccaatttgaa gctggttcag ggatatttaa ttgttcaaat
    10381 atatttttat tgtaagcaat cgcaaacgaa ttcaccccgt tagacagtcc aactaattgc
    10441 ccgtcgtaag tcgtcgttgc taaataggat tcatctgcat tagaaatatc aattatccct
    10501 tgctccacat attcatctaa tggttcaatc tgattgatgt attgaggaaa ttgattgttc
    10561 agttggaaaa tgtcccagat atcatctgat gctgctagtg tacctaattt tgtgtaatat
    10621 ccttcataat cataaaattc ataatcgatc gtcacatggg gatttttttc ttcataaagc
    10681 tcgattactt ttactgttgc atcgtgtcgg acttgcgaac cccaccaagc catgttcagc
    10741 gtgatttttt tgtcatcttc ttgcccacaa gccgtcaaga agaataacat aacacttaaa
    10801 gctaagatga ttctcattct tttcttcatt ctctgcacct ctacttccat tgttattttt
    10861 caatatactg tatttttata cagaacatat ttattaaacg atctttttca aggggaataa
    10921 aaaccaagac ttgccttatc gttctgccta ggatgaacaa ctcaatactt tcaagaaaaa
    10981 ttagatagtg ctgattcttc tttcatcgct aaaaacactt cttgcttcaa tgatgggagg
    11041 ataattacgc aaagaaaaaa ggatatcaga caactgccca atatccttga catagctaaa
    11101 taagcatgct tcttcgtgtg cgctcttccc taaaaaacag aaaaataact agtaattctg
    11161 tatgaaatta ggtacaatat acacgtcgat gcacaagcat tgactatggc aacttgattg
    11221 gtcagttccc gctgactttt caagccccga caatttagct gctactaaat tgtcgggtgc
    11281 cttttttagt ttttattcgt ttttcattct atagaaagcg cttcaataaa tcaagacatt
    11341 tttttgttag aatgctaata ataacaaaag gttttaactc ttcttatgta cattacaggt
    11401 aaaaaaacaa aaaaatttct cttttttttg aattctgaac aaatagtact ggagtacaaa
    11461 agacgaggaa gagccactat ctgctcttcc tcgtcttttg attctcacct gttattcatg
    11521 attctttttc tcagtttccc gatatttttc cattttacga ctaaaaagct ctccgctact
    11581 tggtggcgta tatgtgatgg catttgctcc tgcttgaatg gtctcagcaa tactttcttc
    11641 cgttggacca cctgtcgcaa tgataggaag ttcaggatat tttttacgaa aatggcgtac
    11701 tgcttctgca gtttctttac ctgcactgat attgataatc gaaataccag ctgctaactt
    11761 ttcatctatt tctgtatatt tcgacgttac cgtactgata actggaatat caactactcg
    11821 acaaacttct tttactgtct ctaaaggcgt aggaccgttc aaaacgaccc ctaacgaacc
    11881 ttgcgcttct gcgaacatgc tcatgtagga agaacgagca ccttgtgtca aaccaccacc
    11941 tacaccggag aaaactggaa tgtcagctgc ctcaataatg cttttagtaa tcgctggatg
    12001 aggtgtaaaa ggatacacag caattactgc attcgcattt gtattacgta tgatcgcaat
    12061 atccgtagta aagatgatcg aacggatcaa ttttccaaat atcttgatcc cgcttgcttc
    12121 taaaatgatt tccggtactc ttacgatatc ttttctcaat tccgtcatga tttctggcgt
    12181 aaattgttct gccatcagcc acttctcctt tgctatctcc ggtcatactg attcaattga
    12241 tacgtctcga tgacctggat gattttttgc gcataaccgg ggtctgtcgc ataacctgca
    12301 tcttgcaatg cttgtgcggc ttcctgatag ttggtggccg taatcacttt ttcatacagc
    12361 gcaggattcc aatctactcc ttggacgaac agttgtgtat ggtcatccat ggactgttcc
    12421 catgaatcat acactttgaa gtctccttgg atagttatcc attcttcgtt aacaaattcc
    12481 tttgtttcca ggcttacttt cttctgatcc ccataagcct tgatcccgaa aagattattg
    12541 tatttgctag ccaaggtact ttttccccaa tttgattcta atatagcttg tcctaaaatg
    12601 atactaggaa gcactccata accatcttgt agttcttggg catgcggaac aagccgttca
    12661 ataaattgct gatgggattg cgcttcattt tcttgctgat tatcagtaaa aggagaagac
    12721 agattcttca aggaaaaaac aaaggctaag agtatcactg ctacaccggc aaccactgct
    12781 ggccaacgga tattcttgcg cttttgcttt ttccactttt ttttcgccat gatcaattcc
    12841 tacttttcat ttaattcatt cagatattct tctagcgtca attggtgttt ggtgatatac
    12901 tcagcgtttt ctttaccgac ataacgtaag tgccaaggtt catatgtgat cttagttata
    12961 tcttcgtgtc cttttggata acgaacgata aatccatact gcgcagcatt atctgcgatc
    13021 catttagctc caggctgatc tccgtaactt gcatccaata cagtagaagg ataactgttg
    13081 taccagtttt gatcgacgac atcgactgcc agacctgtgt ggtgttcgct gtatcctggc
    13141 tccgtaatcg tttctttagt aatctttgtc gcctcctctt ctgatacacc ttgacgactc
    13201 atcacatctt ggacattttg ttcgaaaact tgttgttggg atgatacaga ccggtaagct
    13261 gagaccatca ctaatggaaa tccagctgct tcagcagcct tggcaaatgc ttcatattct
    13321 ggagcaatgc gtttgtcgat caagtatccg ttacttaatg atgccaattg agtagactca
    13381 tcaatttctt tttctaattt gtgatctgga ccaaccaaaa ctagcttcca atcgtctgtt
    13441 ttaacatctg gcaattgttt ggacgtacga tcttttgttt ctttgtcctt taccttgctg
    13501 ctggttgttt gactgttctt ttctttacta gcagttgttt tggtgcttgt tcgttcgact
    13561 gcttgattgc cggcttgaac atgcgaatca tcatcgctaa aaataacata gcccattgct
    13621 gcgatcataa tcacaactgc tccaaatcct aaaaatttat tcacttgaaa aatcctttcg
    13681 ttgttcttca cgttccccat tcgcctcatg aacgtaagtt tcttccagat tttcattcac
    13741 ccaatcgaga agttcgattt tttctccttc gatctgtttt tgaacatctg ctggcagacg
    13801 aaaattcaaa atatcatcat agccccattc atcatattga atacttactt tcaagtcaag
    13861 gtatctacca gcctgctgat tatttttcaa agggatcagc tcgacttttg ctgctttgct
    13921 gctaagccat ttttgcgcga tgcgtgtttt gatttgcttg tactctgcaa cggcaacgtt
    13981 tcgtttttcg ggaaaaataa tcgtttgacg caaaatcttt tgcgttagca tccattcata
    14041 atcagtgaaa agatttttta ctttctgcat cgcttcagat ggcagatttt tctcgccatc
    14101 tttccatttt tgccattgtt ctggactgat cagcaagtat tcatctaaaa aatttttttc
    14161 agtttcaaac tgttctaata atacactcat aataagatca atatatactt gatgcacgtt
    14221 tatcccctcc ttttaactat tctacttaag ttttgatcaa aaagcacctc ttaaatcgct
    14281 gctttcattt tttttaatgt ttcaagtcct ctcctcttta ttcttcgtga tttttgttat
    14341 aataatagac agacaagaaa ctcaatccaa ctatgaagga gcgttttcag atgaaaaaat
    14401 atcaatgggg aatcgttggt cttggtacca tcgcacatga gtttgccgaa agcttcaatc
    14461 aagagacgag cgaactaaca gcagttgctt ccagaacttc agaaaaagcg gaaaacttcg
    14521 ctcaccgtta caatattcca aaagcttacg gaagctatca agaaatgtta gacgatgctg
    14581 aaatcgacat cgtgtacatt gctgtaccaa atagacagca tatcgaccat atcttggcag
    14641 cattggaagc aggcaaacac gttctatgtg aaaaagccat cacgatgaat aaaaaagaac
    14701 tggctgacgc tatgcgctta gcagaagaaa agaatttgat cttagcagaa gcgatgacaa
    14761 tcttcaacat gccactttat cagcagttac gctcaataat ggatacagga aaactggggg
    14821 ctttaaaaat gatccaagca cctttcggca gttacaaaga accagaccct aaaaaccggt
    14881 tcttcaatcc agaattagct ggaggagctc tgttggatat cggtacgtat gctgtatctt
    14941 tcgctcgttt ctttttgagc tctcaacctg aagtagttgc ctctacaatg gttccttttg
    15001 aaacaggcgt ggacgaacaa tccgtcacta tcttgcgtaa caaagaaaat gaacttgctg
    15061 ctgtcagctt gacttttcag gcaaaaatgc caaaagtcgg tatcgttgcc tttgagaacg
    15121 gttatgtgac gattgctgat tacccgcgtg ctgatcgtgc ggagatctta tttacagatg
    15181 gtacaaagga attcatcgaa agcgggaata catctgaagc aatgaattac gaaataaaga
    15241 atatggtaca catgattgaa ggtgacttgc ccaatcgctc tctcttcttg acaaaagatg
    15301 tcatcgaaat cttggatcaa atgcaagttc tatggaaaaa tatgtaattt taaaaggttg
    15361 tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc tcctcctatt
    15421 tttcgtttgg ctggacttat taccgcagag gaaagctttt gaacaccgtt taagaaagag
    15481 gttgtgagcc taccgttcag ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
    15541 tgtttgttga atattgtgac ttgtgaccga ggagttgaca ggagaacacc gtttaaagaa
    15601 gaggttgtga aatcagcttt ttgacctgaa tattagctga attcacaacc tctatttttg
    15661 gattttttgt tgggagaatt ctgccttgat ttcaataaaa cttttaaaag actgcaaaaa
    15721 ctggaacaat tctgctcgat cctcgaattc agggcgacta gtcggcagag gcatttgccg
    15781 atacaataca taaacttctt caatacgtgt taaaatttgt ctgccatcat ttgactctga
    15841 aaatgtttct gcagtataaa tcaataaacc atagatatgt tttccataat aagctggttg
    15901 ctggattctc tctagatttt ccagcatgtc tttaatcaca ttggcctgtg ctcttctcat
    15961 agagaagtaa gtttcgtagt atagcggctg tctgagccag tagttttctt gatgttctct
    16021 ggcatcttct tggctttcgc gaatgaatgt cagtaaatgt tcgcatttga tctgcacatc
    16081 ttctttattt tctgataaaa gaaattctgc catttgtctc aaaatatttc gaaattcttt
    16141 ttcgatctgt atttggtttt ccttcagctg cttcgtacga tctggcatgt aaacatttgc
    16201 aagcaaagca aatactaatc caatcgccat taatcccgct tcattgccga tcagctgcca
    16261 agacatgttt tcctccacta aataatgagt gaccaaaacc gaatttacta ctatcccttc
    16321 ggttaatttg aagcgagagg caagcgggat gaacaggaat aaaaaaagac tgaagccaaa
    16381 aatagtatac ccaacagcaa aaaaactgaa cagtgctgtt gccgtagcta atataaaggc
    16441 tgcgattcga ttcatgcccg tacgcaatgt agagcgcttg gtatttccta cacttaataa
    16501 tgcaatgatc cctgcagctg gccaatacaa cagcgataag ctacccgctg tgaccatcga
    16561 aaagaagaca ctgatcactg ttttgactgt tcgcattcct atacgcattt acttccccct
    16621 tttttatttt aattatactt gaataatgaa aaactagcta tcagacaaaa gtttaggtat
    16681 gatagtaaaa agaccaaaaa ggagaatctc tatgcaattt ggccgttttc gactaggtat
    16741 gcgtacgatc aaaagtgcct tagctgtttt tttatgtatc ttgttttttc atttgactca
    16801 acgaggaatc ccgatgattg ctgcattatc tgctgttttt tctttgcgtc aggacttgac
    16861 tacaaccgtt tcttttggaa agtctaggat catcggcaat acattaggtg gtacattagc
    16921 tatcatttat ttttttgtca aaagctattt tcacaatgat tttttggtag aattgatctt
    16981 gcttcctatt ctagttatta ttgtgatcgt cgtgtcagat ggtattaaca ataattccgg
    17041 tatcatttcc gcaatagcta cgttgttgct gattgcgttg agcatccctc aaggcgaatc
    17101 cgctttgtat gctatccaac gtgtactaga tacttttata gggacattca ttgcaatcgg
    17161 tatcaatttc tttcttcgtc ctcctgaaac agaaaagaag gaagaactat cagaagattt
    17221 ggtcgaactc caaaagaaag aacgattgct aagaacaaat ttacaagaga tagaagctaa
    17281 gatccaaaaa caaaagaaaa atagcgagga ctaattttct tttgcaataa aataaaaaaa
    17341 tggagagaag aactttaaaa agtcttcctc catttttgat tgaacggtga agtgtactac
    17401 tttat
//
LOCUS       DAJSVF010000052        17184 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSVF010000052 DAJSVF010000000
VERSION     DAJSVF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.054"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(543..2048)
                     /locus_tag="ON550_001782"
     CDS             complement(543..2048)
                     /locus_tag="ON550_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3954867.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLFENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ON550_001783"
     CDS             complement(2045..2731)
                     /locus_tag="ON550_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954868.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ON550_001784"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ON550_001784"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR3954869.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="ON550_001785"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="ON550_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3954870.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="ON550_001786"
     CDS             complement(4752..5321)
                     /locus_tag="ON550_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR3954871.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="ON550_001787"
     CDS             5507..6409
                     /locus_tag="ON550_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3954872.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="ON550_001788"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="ON550_001788"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR3954873.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="ON550_001789"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="ON550_001789"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR3954874.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="ON550_001790"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="ON550_001790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR3954875.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="ON550_001791"
     CDS             12977..13162
                     /locus_tag="ON550_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR3954876.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="ON550_001792"
     CDS             complement(13224..14420)
                     /locus_tag="ON550_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3954877.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="ON550_001793"
     CDS             complement(14590..16026)
                     /locus_tag="ON550_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3954878.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="ON550_001794"
     CDS             16319..17128
                     /locus_tag="ON550_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3954879.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5043 a         3594 c         2983 g         5564 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa acagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tttt
//
LOCUS       DAJSVF010000053        17106 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSVF010000053 DAJSVF010000000
VERSION     DAJSVF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.055"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            287..694
                     /locus_tag="ON550_001795"
     CDS             287..694
                     /locus_tag="ON550_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954880.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ON550_001796"
     CDS             708..1109
                     /locus_tag="ON550_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954881.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ON550_001797"
     CDS             1111..1482
                     /locus_tag="ON550_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954882.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1823)
                     /locus_tag="ON550_001798"
     CDS             complement(1518..1823)
                     /locus_tag="ON550_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954883.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     LHYSNKNYFKIPAVKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2350..2422)
                     /locus_tag="ON550_001799"
     tRNA            complement(2350..2422)
                     /locus_tag="ON550_001799"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2387..2389),aa:Lys,seq:ctt)
     gene            2803..3372
                     /locus_tag="ON550_001800"
     CDS             2803..3372
                     /locus_tag="ON550_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR3954884.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3359..4219
                     /locus_tag="ON550_001801"
     CDS             3359..4219
                     /locus_tag="ON550_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3954885.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4409..5113
                     /locus_tag="ON550_001802"
     CDS             4409..5113
                     /locus_tag="ON550_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3954886.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5118..6953
                     /gene="walK"
                     /locus_tag="ON550_001803"
     CDS             5118..6953
                     /gene="walK"
                     /locus_tag="ON550_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR3954887.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6950..8266
                     /locus_tag="ON550_001804"
     CDS             6950..8266
                     /locus_tag="ON550_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954888.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8267..9136
                     /locus_tag="ON550_001805"
     CDS             8267..9136
                     /locus_tag="ON550_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR3954889.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9191..10000
                     /locus_tag="ON550_001806"
     CDS             9191..10000
                     /locus_tag="ON550_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3954890.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10103..10732)
                     /locus_tag="ON550_001807"
     CDS             complement(10103..10732)
                     /locus_tag="ON550_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR3954891.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10902..12194
                     /locus_tag="ON550_001808"
     CDS             10902..12194
                     /locus_tag="ON550_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3954892.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12270..12932)
                     /locus_tag="ON550_001809"
     CDS             complement(12270..12932)
                     /locus_tag="ON550_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3954893.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13157..13441
                     /gene="groES"
                     /locus_tag="ON550_001810"
     CDS             13157..13441
                     /gene="groES"
                     /locus_tag="ON550_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR3954894.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13492..15117
                     /gene="groL"
                     /locus_tag="ON550_001811"
     CDS             13492..15117
                     /gene="groL"
                     /locus_tag="ON550_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR3954895.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(15206..16354)
                     /locus_tag="ON550_001812"
     CDS             complement(15206..16354)
                     /locus_tag="ON550_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3954896.1"
                     /translation="MLKVWVEQTKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFDELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYSSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRLTDPDQYIFISTSGTPLTLHSFNAVLHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(16456..17040)
                     /locus_tag="ON550_001813"
     CDS             complement(16456..17040)
                     /locus_tag="ON550_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR3954897.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         5659 a         2874 c         3492 g         5081 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtac ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctcgttttc acagcaggaa ttttaaagta gttcttatta
     1681 gaataatgga gattgactag caggctagtt tcaattgcta aaaagactac ttcagtatcc
     1741 cagactttat ataaatcttt gacaaatttt tctgatggat cgtatttaaa ccaaagcaga
     1801 tgttcctcta gctcgattgt catgtttttc acctcaacga aagtatacga actaatgttt
     1861 gttttgtaaa ggataaaaat agggtagcaa ccggtaccca aactagtacc catttatcaa
     1921 aaacgacgaa atttatgtaa atagaaaaat aagaataaac attgatgtat caacatttat
     1981 atatctttag aacaaatgaa aagggtggtt ctcataagaa aaacatgttc gcacttctag
     2041 gcaaaccagg atttgaagac cttgctaaag aattgaacga acgcctgtaa aataaggatt
     2101 tgtcgctaga tttaaaaaat cagtgataga aacatgtttg ctttaaaatg aaactagtga
     2161 tagttttagt gatagtgaac ttaaaaaaag cactcaattt gagtgctttt ttattgttat
     2221 taaaaattaa aaaactaaaa tacatacccg aatttcatac ccgaaatata aaaaatgtca
     2281 tcacgtacat ctttttcgaa actaccgaag tagccaaaga tgtactgatg acgtgggttt
     2341 ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga gtcagctgct
     2401 ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca ctaagaattt
     2461 taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt ataggaaatc
     2521 attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa caaagttctt
     2581 gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc gtctccccgg
     2641 cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt tttttgtttt
     2701 catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg tttatcataa
     2761 gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag
     2821 caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg ggaagaactc
     2881 cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta tctttcacct
     2941 gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa ttacgttaaa
     3001 ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt agcattttta
     3061 cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga agggatattg
     3121 ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga tgaacaagaa
     3181 gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca aaaattcgac
     3241 caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa
     3301 cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga gaaaacaaat
     3361 gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag aagaattgat
     3421 aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc aggtatatac
     3481 agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg aaatccctgg
     3541 tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca aaaaagacgg
     3601 ttatgaaaaa gtacttgcgg taacaatttc cagtggattg agcgggacat ataatgtggt
     3661 tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata cgaaaaatat
     3721 tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag aaggagtaga
     3781 atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag ttttctttaa
     3841 tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg cttctattgt
     3901 aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga tttattacac
     3961 agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt acgtcaaaga
     4021 aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg cgaaagaaga
     4081 agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc aaatctactt
     4141 tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg gtgtcggtgt
     4201 acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc
     4261 ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat tttaattttc
     4321 tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat gagaaggtat
     4381 gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa
     4441 gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg tttatactgc
     4501 atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt tgattttact
     4561 tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac gcaaaactta
     4621 tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag tcctaggctt
     4681 agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat tagttgcacg
     4741 tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag aggcagcacc
     4801 tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg tgacgaaacg
     4861 aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact tggcgaagca
     4921 ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt atgattattt
     4981 tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa
     5041 tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc
     5101 agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc aattttaaga
     5161 tcgctattac ctttattctg attttattga tctcgatcga aattattggt gcctacttta
     5221 tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat caaacggtgg
     5281 aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca gatgatgaag
     5341 aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat atcatcgaaa
     5401 tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg
     5461 ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa cgttatgttg
     5521 ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg tccccgaccg
     5581 gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa
     5641 tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt atttcaatga
     5701 tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg agagaacaag
     5761 ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga caagatgaac
     5821 ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa gctcaagata
     5881 caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca gacggcgtca
     5941 ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt tccttgctag
     6001 atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat atcgaagaag
     6061 aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac cgttcaacca
     6121 gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc cgtcgcgaat
     6181 caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag
     6241 aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca ccgttgacaa
     6301 gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac
     6361 caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg atcaatgacc
     6421 tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa tatgtcaatt
     6481 tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga aatcaggaaa
     6541 aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa gtagatacag
     6601 ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat tcaccagatg
     6661 ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta agtatcaccg
     6721 atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag
     6781 tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct atttctagag
     6841 aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga aaagggtcga
     6901 ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa tgagaatctc
     6961 tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat acttctctta
     7021 tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga caaacactca
     7081 aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac
     7141 atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt accgattgca
     7201 attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag gagacaaatc
     7261 acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata atgcgccttt
     7321 tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga acttggcaaa
     7381 agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata aaaaaatccg
     7441 atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga attggacaga
     7501 tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa cagaaccatc
     7561 gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt atagttatat
     7621 cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac cagatagcgt
     7681 gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa caatgtctat
     7741 ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa aagaaaatgc
     7801 tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt tagggaacct
     7861 cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg aaggcttccc
     7921 ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg aaacgaatgg
     7981 ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga taccttcaga
     8041 cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag ctaagggagc
     8101 agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg tcaaagaaac
     8161 gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata actggtattc
     8221 agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg atttcaaaaa
     8281 aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat tcagcagtta
     8341 tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg aatcaatcca
     8401 acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg agaacaagca
     8461 aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga attatcgcaa
     8521 ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa
     8581 atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc
     8641 cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt atgtgtacac
     8701 gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc aagagtatga
     8761 aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa aaaacgataa
     8821 tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc agctgcgcga
     8881 aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca ataacaaaat
     8941 accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc tgaaagtccg
     9001 ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt
     9061 ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa
     9121 agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta agaagggaag
     9181 taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag caagtggaag
     9241 ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact
     9301 tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg accaattaga
     9361 tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg tcttggctcg
     9421 taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg aaccactgat
     9481 tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca gaacattcgg
     9541 cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc aattttatcg
     9601 tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt gcagtgatta
     9661 tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc atgatcttga
     9721 gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag gagatagagg
     9781 acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta atcggaccaa
     9841 acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg ctcatttaac
     9901 aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg aaatctatga
     9961 tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat caataaaaat
    10021 ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt tattttttta
    10081 gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc ttttcatgtt
    10141 tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt
    10201 cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct gtacgtttgc
    10261 catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt ttagattgtt
    10321 tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt gcatttttag
    10381 gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc
    10441 caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg gacaaagaag
    10501 tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca acatcaaaag
    10561 atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga acatctgatg
    10621 gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac aatgcttcgt
    10681 ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc ataaaaaacc
    10741 tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt
    10801 ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata gttaaattaa
    10861 gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag tggttagatc
    10921 gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact gctttgcgta
    10981 atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc ttatttgctc
    11041 attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct atgtatttta
    11101 cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt gccattgcat
    11161 acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca gccttgtctg
    11221 cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg acaggtgctg
    11281 aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat gcacgtttag
    11341 taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt tggcagtctt
    11401 ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt tcagcggttt
    11461 ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc cagcttccca
    11521 ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt gttccgtttt
    11581 tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt cccgtcttgc
    11641 aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg acggtggcaa
    11701 atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt acgggtgctg
    11761 gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag aaatacagag
    11821 aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca attatttttt
    11881 ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca ccggtcgttt
    11941 caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt acagcagtcc
    12001 aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga gtgcctgcag
    12061 cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca tttttgaaaa
    12121 aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa
    12181 agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa atcaccaatc
    12241 taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac agctaattga
    12301 acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca gatccgacca
    12361 gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag cagatgacca
    12421 tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca aaaatttgaa
    12481 tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat gatgggtcca
    12541 gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat gatattttgt
    12601 gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac agcgatgctt
    12661 tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt tggagcagaa
    12721 gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata aaaaatagct
    12781 aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc agacgtagat
    12841 tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc atagcaaaaa
    12901 atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag ggataacaag
    12961 catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa taagcagctg
    13021 tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga gaatgagtat
    13081 tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa aaaaagagtt
    13141 atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa
    13201 agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag agaaacccca
    13261 aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc
    13321 tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta cagaagtaaa
    13381 atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga ttgttgagta
    13441 aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa
    13501 gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga taaattagca
    13561 gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga aaaatcttac
    13621 ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga attagaagat
    13681 cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac aaatgatatt
    13741 gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg cgaaggttta
    13801 aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga attggcaaca
    13861 aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa agaagccatt
    13921 gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat tgctgatgca
    13981 atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca
    14041 gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca atatatggta
    14101 acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat cacagacaaa
    14161 aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca acaatcacgt
    14221 ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct tgtattgaac
    14281 aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt
    14341 aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac tgacgatcta
    14401 ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa agttgttgta
    14461 gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt
    14521 gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg cgaaaaatta
    14581 caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg tgcagctact
    14641 gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc tacacgtgcc
    14701 gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa
    14761 gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct
    14821 ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc agtgattgtt
    14881 gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg agaatgggta
    14941 aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc cttgcaaaat
    15001 gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga taaaccagaa
    15061 ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggcat gatgtagtaa
    15121 ttaagaaaga accctatttt aacagggttc tttcttcttt ttttgtttta ttttaagtaa
    15181 aaggggcaaa gaaggggcaa aaatatcaaa ggctatttag tttatcaatt acttgttgtt
    15241 tggctctttt ggttacatgg ttatagatag acagagttgt atttgcatca gaatgtccta
    15301 cacgttccat aatggcttta agaggtacgc ccaattcaga taatagtgaa acatggctat
    15361 gtctaaatat atgcgatgat agactttttt ctaactccaa ttcttcctct actttatgga
    15421 gtacagcgtt aaatgagtgc agcgtaagcg gagtgccact tgtagatata aatatatatt
    15481 gatcgggatc tgtgagtcta cccgcaagaa tgttgtcagc tatcacactt tcaatcaatt
    15541 cttttgcacg attgggcaat tgtacttcgc gttgcgaata agtatttttt ggagttgttt
    15601 ttatagcatt atccattttc acagatgagt aatctaaggt accgttaatg gaaattttcc
    15661 catcctcata gtccttcatt tgtaaagcta gcaattcccc atatctcaaa ccagtcaaat
    15721 ataaaaattc agctattatg ccgtgtagtt ttctgcgagg attggagtat agctgtttca
    15781 gtatttgatc aatttcttct ttctccagat attttttatc catagaaagc tttctttttt
    15841 cttcttccac ttttttaggg tggattttaa ctgctagagc agggtttctt tggatgtatt
    15901 ttctatcgat tgcatagttt agcataacag atagagttgt ttttgtttgt tttgtgtagt
    15961 tcagtgagag gtcaccaaac gtatacatat cttcaattat cttattaatg agtgtctcat
    16021 ctatgtttct aacgattgta tcatcgctta tgtgcttaga aacatgtttc atcatcattg
    16081 gcaccttcag atagctagta cgtttaacat gctgcttata atattcatac cattctttat
    16141 acagctcatc aaaagtgata tctgatttat tgtaattttc gagtgcttct ttaatttttt
    16201 tatctagaat tttctgagct tttttccacg cttgtggtga attacttgta agtgttgtag
    16261 atttttttcg tgttttttct gtataaggat ctacatatct ttcaataaac ttgaatcgcc
    16321 catctttggt ttgttcaacc cacactttta acatctccta tcatttgcta taataggcat
    16381 aacaaataga cctatatagg tttgttttct aaaagcacgc tcttactctg gacggtgggg
    16441 cgtgtttttt atttattata cagttgcttt aaaaatagct gctttttcaa atgatactaa
    16501 aggagagaag tggatttcta tttctccctt ttggtttaac gtgaaatgag cagttacatc
    16561 catttgtttt cctggagcaa cagatcccat tgtattttcg ttggcatatg tctctgattt
    16621 tttgtcatct ggtccatata cttccacatc tgtacctaca gggatatctg aatcgccatc
    16681 attttttacg gtataagtaa tttttactac ttgtgcaggt tggttttctt caaattgatt
    16741 tctttcatca gttagttcta cactatttag tgtatattca gcatcaccaa cttttacagt
    16801 gtcaccaatc ttatagaagg tatcgctttt ttcttcttta gaagatgaag cagaggttga
    16861 agatttagtt actttttcgc caccattatc acttgcttta ttactatctg atccaccatt
    16921 taaagcagaa ccaataatta taattaaaat tactgctaat acccaaaacc atactttttt
    16981 gtaaaaaggt ttactaactt tatacatttt tccgtcttga cccataactt tttttgccat
    17041 ttaaatattc ctcacttctt gttataatat atttgcgatc tcagaaatga ggtatgagtc
    17101 cgtgtg
//
LOCUS       DAJSVF010000054        16727 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSVF010000054 DAJSVF010000000
VERSION     DAJSVF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.056"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..241)
                     /locus_tag="ON550_001814"
     CDS             complement(<1..241)
                     /locus_tag="ON550_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3954898.1"
                     /translation="MVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYELDPSPLI
                     NRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTA"
     gene            complement(263..460)
                     /locus_tag="ON550_001815"
     CDS             complement(263..460)
                     /locus_tag="ON550_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR3954899.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(854..2581)
                     /locus_tag="ON550_001816"
     CDS             complement(854..2581)
                     /locus_tag="ON550_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR3954900.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2574..3767)
                     /locus_tag="ON550_001817"
     CDS             complement(2574..3767)
                     /locus_tag="ON550_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3954901.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3954..4598)
                     /locus_tag="ON550_001818"
     CDS             complement(3954..4598)
                     /locus_tag="ON550_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3954902.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4692..4826)
                     /locus_tag="ON550_001819"
     CDS             complement(4692..4826)
                     /locus_tag="ON550_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR3954903.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4828..6960)
                     /gene="feoB"
                     /locus_tag="ON550_001820"
     CDS             complement(4828..6960)
                     /gene="feoB"
                     /locus_tag="ON550_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR3954904.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6957..7430)
                     /locus_tag="ON550_001821"
     CDS             complement(6957..7430)
                     /locus_tag="ON550_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR3954905.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7831..8055
                     /gene="nrdH"
                     /locus_tag="ON550_001822"
     CDS             7831..8055
                     /gene="nrdH"
                     /locus_tag="ON550_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR3954906.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8214..10373
                     /gene="nrdE"
                     /locus_tag="ON550_001823"
     CDS             8214..10373
                     /gene="nrdE"
                     /locus_tag="ON550_001823"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR3954907.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10423..11388
                     /gene="nrdF"
                     /locus_tag="ON550_001824"
     CDS             10423..11388
                     /gene="nrdF"
                     /locus_tag="ON550_001824"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR3954908.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11477..12616)
                     /locus_tag="ON550_001825"
     CDS             complement(11477..12616)
                     /locus_tag="ON550_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3954909.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12925..13638)
                     /locus_tag="ON550_001826"
     CDS             complement(12925..13638)
                     /locus_tag="ON550_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR3954910.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13892..14593)
                     /gene="nagB"
                     /locus_tag="ON550_001827"
     CDS             complement(13892..14593)
                     /gene="nagB"
                     /locus_tag="ON550_001827"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR3954911.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14827..16359
                     /locus_tag="ON550_001828"
     CDS             14827..16359
                     /locus_tag="ON550_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3954912.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16550..>16727)
                     /locus_tag="ON550_001829"
     CDS             complement(16550..>16727)
                     /locus_tag="ON550_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3954913.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5193 a         3443 c         2884 g         5207 t
ORIGIN      
        1 cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc
       61 ttattttatt agtataagca gtaaaatata gataattcac caatctattt attaaaggac
      121 tgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
      181 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
      241 tttgcttagc atttttttca ttttagcact tccctggaat tgctccacct aaaaaaccac
      301 ctatagacat tcctgcaata caagtagttg ccttggccca atcgaccgta catttatttt
      361 tagtgcaata cactccattt ccataatatt ttccactatg agtggtaccc ccatagataa
      421 gttgtgtctc tttaatagac aaaattttta aatgtttcat aatctaatca cccctaaatt
      481 aatgttttta gactattatt ctaacattca ttaaatttca aaaaacaatt ccttcttgaa
      541 acgtagcaaa ataattcctg aataagaatt taatcacttt taagtatata aactatgtat
      601 attctttaat attttttgag aatgcacttc gtacataata aactttaatc acattagtct
      661 ttcataccta cgatcgatct ctttgactcg cacccataaa ttcataatag tttgtcggcg
      721 tctttgaacg tttcgaagat gagctacgca ttctcttttt ttatagacaa aaaagataga
      781 gacgaacatg gagcttccaa atttggatat ttgatccaat tttggaagct ccatttattt
      841 cgtgtctgtc tttttatttt ttccagcttt cgactaattc ttggttctct ttgatccagt
      901 cttttgctgc ttgctgtgga tcttttccat tgttgatttc caacataact ttttccatat
      961 ctttttctga ccaatggaaa ttgtctaata ctttgtaagc ttctggctgg tcttctttca
     1021 atccttttcg aaccatcgta tggatctgct cttcgcttcc cattccgttt tcaggatctt
     1081 ctaagtattt cagatcatat ttggcaaaca tccaatgagg tgtccatcct gtaactacaa
     1141 ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt cattgctcct gaagaagatg
     1201 tctctacttt ccagtctttc aagttgtcat atttttggat cgtgttttca gcagccgtaa
     1261 ctacaccagc tcctggctcg attcctatga ttttcttacc tgcctgatcg gttaactcat
     1321 cgatcgaatt tacatccatg taggctggca ctgctaaacc aacttttgct cctgtcaaat
     1381 tggcacctag gtcttcgact tgatctttat attgtgcata ttgtgaacca tgagttttag
     1441 gtagccaagc tgaaaccatg gcatctgact cgccatttga aacagatttc cacatgattg
     1501 aattatctaa tggcgtcatc gttacatcat agcccatttg tttcaatact tcaccgacta
     1561 catttgttga agcaacttct gtatcccatt caacataaga taagttgata cgttttgttt
     1621 ctttggcaga tgaaaaactc cctataccaa tcgcacctaa gatcactact actacagcac
     1681 caatgatcag tccttgacgt tttttacttt tctgtgttgt tgctcctgca gcttttttat
     1741 tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat gattgctaag ataactaatg
     1801 caaccccgtt gacaaatcca ttcccgactt gtgctctttg caatgctgat aatacgccgc
     1861 gtcctaagcc gggtgcaccg atcatagagg caataacgac cattgaaagt gacagcattg
     1921 ttgtttgatt gatccctgcc atgatcgtac ttttagctaa aggcaattct agtttgaata
     1981 gtttttgacg acttgtgctc ccgaatgaat cagaagcttc tactaattct ttaggaactt
     2041 gtcgaatccc taaattcgta aaacgaacgg ttggtggcag agcaaagatg actgaggcaa
     2101 atacccctgg aaccatcccg ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc
     2161 ctggcattgt ctgcataaag tcaaggatcg gtttgataat actttgggct ttactgcttt
     2221 ttgccatcaa gatccctaat ggtaccccaa tgatgatcga gatcacacta gataaaagaa
     2281 caagtgtgac agtattcatc aaatcattcc atagaccttg gttataaata aaccatagac
     2341 cgattaatgt gaacaacgta agtcctggac gtttcttaga aataaagaaa gctgctaccg
     2401 tcaataacaa gataaacaaa ggagctggaa tcactaacag taaatttgta atccccgaca
     2461 tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc
     2521 attctgtgat attttctacc cagtttgcca ctggtaattg atagttcaat agattattca
     2581 tgttcactca cctcgcttgt ttctgctaat gcttcaatca cactacctcg gatgacgaca
     2641 ccttttaatt ttccatcctc tacaactgca agtggagtcg gtgagtcata aataatattg
     2701 aaaatatcac ttaccaccat gtctttccca atcaccgtca cattttcatc taggaagtcg
     2761 atcaatggac gtttttgctt cctagcttct aacgcttgat ctgcagtgat gatccctttc
     2821 aggtgtctct ttctgtcaac agccatcagc atgctgactt cttcattacg catccttgtc
     2881 aatgcaacgt ttggtccatc agattcgata ttcgtcgtca atgcaggtac catgatgttt
     2941 tgagccgtaa gcactttgga acgatcgact tcttccacaa attcacgtac ataatcattt
     3001 gctggattgg tcaatatttc ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc
     3061 aaggcgattc tgtccccaat ccgtaaagct tcattcaaat catgggtaat gaaaataatc
     3121 gttttttgaa cattggcttg caggtccagt aattcgtctt gcatttctcg ccggatcagc
     3181 ggatcaagag ccgagaacgc ttcgtccatc agcaagatct caggatcatt tgctaaagct
     3241 ctggctaggc caacccgttg ttgcatccca ccagataact gactaggata ttggtcttta
     3301 aatgaaagca aactagaatt ttctaatgct ttttctgctt tttcttgtcg ttcttcttta
     3361 ggtacaccac ggacttctaa cccgtattct gtattttcca agatcgttcg atgtggaaac
     3421 agaccgaagt tttggaaaac catattgatt ttatgacgac ggacttctcg caattcttct
     3481 ttgcttaact tcgatacatc ttctccatca atatagatac tgcccgaagt tggctcaatc
     3541 agacgattca atagacggat caatgtggat ttcccactcc cggaaagccc catgatcacg
     3601 aagatttctc cttcttttac gtcaaaattc acgtcataca caccaaccgt tgcaccggtt
     3661 ttctttaaaa tctccgtttt cggctggtga tcattcatca tatctaaagc ggcttgtgtc
     3721 tttttaccaa atatctttgt taaatgtttt acttctactt ttaccaaaca aattctcctt
     3781 ttttatgtaa attcattttt ctttctgaaa aaagtgacca cactatgtta acatcatgta
     3841 gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat
     3901 ctttgtaaat aaaaggttta gccaagttaa tattttttta atatttcaca caatcattct
     3961 gcaagctgat gctccgggta aaaaaagttt gtcatacgct gtagtgtata ttcatttcca
     4021 ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg ggccaggtga aacaagcagt
     4081 tcttcattat gagaaattgc gataactgtg gcgccagtcg cctgccagat attcaactcg
     4141 ctaattgaac ggtccagatg cattgctttt tctgataaag ataattcaaa cggaagtaaa
     4201 ggattcatct tatgtattcg cttcgtttga ttgaccagct gatccaataa ttgagaaaaa
     4261 tgattcagct catcttgttg cttctccaca cttgccatga tttctgcttt tgtatcttgg
     4321 atggattgta catcttgata ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca
     4381 aatgccccac ttccatgccg tacttccatg attccaagat ctaccagcac attgatagcc
     4441 ttgcgggtag tctcaggaga agcattgtac gtactggcaa gtgttgagcg ggcatggatc
     4501 ttctcaccca ctttatatcg gttttcaacg atacgttcgg ctaaatcaac agcaatctgc
     4561 tggtagcgcg ggcggacgat ttttttcttt ccagacattt tctttcctct ttcttttttt
     4621 actttccctc atcttactat gctctagcaa aataagcaaa aaaggggaga atagcctctt
     4681 gcaacgactg atcatcgctt gatctgttcc ttttttactg ggcaagctgt ttgacagtca
     4741 tcacaattct taccggattt taaacgagta tagacaattg cgcctgcact tccaaagatt
     4801 aacacactta ataagaatgt cgccatccta ttcctctcct tctaatgaca atatagtata
     4861 aaactggttc ttggattttg gctgacggaa caaaacataa acgagtccta tcatcagtaa
     4921 aatagcaaga tacgtcccta acgctacctg tccaccctcc aataagtgtc ccaactgata
     4981 aatcacaaaa ctaactaaat aagctaaacc gcattgatag cctatggcga tccaagtcca
     5041 tttcttggca ttcatttccc gatggatagc cccaattgcc gcaaaacaag gcgcacaaag
     5101 taaattgaag gctaacaatg aataagctgc tgcgggagta tatgtgtgtt gtagcacttg
     5161 ccagatttct ctgccatttt cggatacttc cgcatgacca aacaaaatac caaatgtgcc
     5221 aataatattt tctttagcga ccaatcctgt aatcgctgcg actgttcctt gccagtttcc
     5281 ccaaccaagc ggctgaaaaa caactgccag gattcgcccg aaaaaggcta agatactttg
     5341 gtcttcagaa acaggttgag cataaaaatt ataagaagag ctgaaccaga tcacgatact
     5401 agaaacaaag ataatcgttc ctgctttttt aacaaaagac ttgctgcggt cataggtttg
     5461 tcttaggacc gctgaccatt gaggaaaatg atatgcgggc aattccatga taaaaggagc
     5521 tgtatctcca gcaaacaaac gtgttttttt caagctgatt ccagagaaaa taattgcagt
     5581 cataccaaga aaataggcag aaggagctac ccaagattga tgaggaaaaa aggcccctgc
     5641 tactaaggca ataatcggca actttgcaga acaaggcatg aaggttgtga ccatgatcgt
     5701 caaacgacga tcttgctcgt tttcaatcgt gcgactggcc atgacgcctg gcactccaca
     5761 tcctgtagca atcagcatag gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag
     5821 tcgatccatg acaaaagcga tcctcgccat atatccgcag tcttccagaa agctcaagca
     5881 taaaaacaat ataatcaatt gcggtagaaa tcctaacact gctcccacgc ctgcgatgat
     5941 gccgttcaag atcagctcct gcatccatcc cgctacttgc cattcagcta acaatctgcc
     6001 aacatgttct ggtaaccatg taccaaacaa tgtgtcattg atccaatctg tccccattgt
     6061 tcctactgtt tgaatagcca gataatagat cagccacatc acaaatgcga agatcggcaa
     6121 ggctaaccaa cgattcgttg caatctgatc gattttgtca ctcatcgata attggaaaga
     6181 atcatttgac acaacacaga gtgtacatag ttgggtaata aattcataac gttcattgac
     6241 cagaatcgtc tcactatcgt cgtccaatag tttctctgtt aactggatca gttgctcgat
     6301 ctctttttgc tgagaaactg ctatctcgta atcctctctt accttctgat ctcgttcaaa
     6361 atatttcatt gcataccagc gtttcttgcc gctttgttct cctagtacat cttcaatttc
     6421 atgtaaagct gcttctaaac gtggatcata tgtacaggga acaggaatag aaggccgctt
     6481 ttttgcagac tcctgaatct tttcgattag tttttcgatt ccttgctttt tcaatacgct
     6541 gattggtaaa actggtacac caagaccata ggaaagcttt tctagattga ttttttttcc
     6601 ttcttttttg atgacatcca tcatattcag accaatgatc ataggaatgt ctaaatccaa
     6661 taactgcgta gttaaataaa gatttcgttc taaattggta gcatcaataa tgttgatgat
     6721 cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc acttcttctg gtgtataagg
     6781 agacaaagaa tagatgccgg gtaggtcctg aatatgaatg ttcggatttt ttttcaacga
     6841 tccttctttt ttttctactg ttacgcccgg ccaattccct acatattgat tcgaaccagt
     6901 caagagattg aacaagctag ttttcccgct gtttggattt ccgactaacg cgatattcat
     6961 tctttttcct ccctttgtat caagattagt tcggcctctg actttcgtaa cgtcagttca
     7021 taccctcgca ctgtcagttc gattggatct cccagagggg ctagttttcg gataactatt
     7081 tttacatttc gcgtcaatcc catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg
     7141 attgctacga cgatcccagt ctctcctgct tttaattgat caagagaagt ccagctttca
     7201 gaggaagcag tctttttatt gacaaagatt tgacctagta tttcccgact tactgccact
     7261 cgatgacttt ttaccagtaa aatggcattg ttgcctgata aattgacgac gacgatttta
     7321 cttccggcaa cgatcccgat attttgcaaa tggcgttttg ttttttcttc tgtttcgatt
     7381 cgttcgactt gataaacgaa attctgcttt gcctgcgcta aggtctgcat attgattcct
     7441 ctcttgtgaa ataaatcatg tattaaaatc attcattata tgttctaatt ttcttacgtg
     7501 ttacgcaaat aaatgagaat cattctcaca tagaatactc tcggtaattg agaatgtcaa
     7561 ttatacttgt gatattatga gcgaatcttt agtattgata gataaaaatg agaaaaaaat
     7621 atttcttcat agttttccac aattttttct ctattaaatt tgctattttc ccacacgata
     7681 cttgttttca gctctttttt caaaaacaca aaacttagta ttccacagtt tccacaacac
     7741 aatatgtagt gtttgttgtt tgactttttt actaagagtt ttatactatc tattatagat
     7801 aataagaatg tgttagagga ggcactcacc atgaacgtaa aaatcttttc taaaaataat
     7861 tgtatgcaat gtaagatggt caaacggttt ttaacagaaa atcaaattgc ttttgaagaa
     7921 gtcaatatcg atgaacagcc tgaagcagta gactggttga aagcacaagg attccaaagt
     7981 gttcctgtca ttacttctga tgcagaaaca gttatcggat tccgtcctga tcagttgcgt
     8041 aaacttgcca gctaacatga ctaaaagaat caaaacgtga tacatggatt tgaagggctt
     8101 gagacagcac cagtttttgt cgcaaacttc caaatccttt tttaaagctt aaaaacaatg
     8161 caatgatttg caatcaagca tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc
     8221 ttaaaaattt acaagatgtc agttatttca agctgaataa tgaaatcaat cggccagtca
     8281 atggtcaaat ccctttgaat aaggataaag aagctttagt tgcctttttt gaggaaaatg
     8341 tcaaaccaaa tacgatgact tttccatcta ttatggaaaa aatcaattac ttgatcgatc
     8401 aagaatatat agaagcagaa ttcattcaat tgtattcacc atcatttatt gaaaagctat
     8461 atcaattttt ggacgagcaa catttttctt tcaaatcttt tatggctgct tacaaatttt
     8521 attctcaata tgcgttgaaa accaacgacg gttccgctta tctggaaact tatgaagatc
     8581 gagtagcctt caatgcgctt tactttgcga acggggatga agaattagca cttgctcttg
     8641 cagacgaaat gatccatcaa cgatatcagc cagctacacc ttctttcttg aatgctggac
     8701 gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca agtcactgac gatatgaact
     8761 ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg tattggtggc ggcgtaggga
     8821 ttactttatc aaatcttcgt gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa
     8881 gtggtgtcgt tccagtcatg aaattattcg aagatagttt cagctattct aatcaattag
     8941 gtcagcgtca aggagctggc gtggtttatt tgaacgtttt ccatccagac attattgctt
     9001 tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg cgtaaaaact ctttctcttg
     9061 gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa aaacgaagat atgtatttat
     9121 ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt ctcttatgta aatattacag
     9181 ctgagtatga tcatctagtt gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg
     9241 atcttgaaaa tgaaatctca aaattacaac aagaatctgg ctatccttac gtaatcaata
     9301 ttgatacagc gaaccgcgag aatccaatcg atggcaagat catcatgagt aatttatgtt
     9361 cagagatttt acaagttcaa acgccttctg tgattaacgg caaacaggaa tatgaagtat
     9421 taggaacaga tatctcatgt aacttaggct cgacaaatat cgtgaatttg atgcaaagcc
     9481 ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt gacatttatt acagatgcat
     9541 ctgatatcga tgtcgtacca ccgatccaaa acggaaataa gctgaatcat acgattggat
     9601 taggtgccat gggcttacac acttatcttg caaaagaaca aatggaatat ggctcagagg
     9661 attcattaga ctttacaaat atttacttca tgctgttgaa ttattggaca cttgttgaaa
     9721 gcaacaatat cgctcgtgaa agaaaacaag ttttccataa ttttgaaaaa tcagcttatg
     9781 ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag
     9841 tcaaagcatt gtttgaaggc atttttatcc caactgctga agattggaat gctttgaaac
     9901 aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct
     9961 caatttcata tatcaacgat acgagtgcaa gtattcatcc tatcactcgc atgatcgaag
    10021 aacgccaaga gaagaaaatc ggtaaaattt attacccagc gccttatcta tccaatgaga
    10081 cgattccgta ttacacttct gcttatgata tggacatgcg taaagtaatc gatgtttatg
    10141 caacggcaca acaacacgtc gatcaaggca tgagtttgac tttattcatg cgttctgaaa
    10201 tcccagaagg catttatgaa tggaagacaa caacgaagca aacgacacgt gatttgaata
    10261 ttttaagaca ttacgctttc cacaaaggaa tcaaatccat ttattacgta cgtaccttta
    10321 ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata
    10381 gatagattcg cttattcata ttttaattaa aggagcattc tcatgtcaga aacatattat
    10441 gaagcaataa actggaatga aattgaagat atcattgata aatccacttg ggagaagctg
    10501 acagagcaat tttggctaga tacacgtatc ccactttcaa atgacctaga tgattggcgt
    10561 acattgtctc aattggaaaa agatacagta ggacatgtat ttggcggttt gacactgcta
    10621 gatactgtcc aatctgaaag tggcatggat cagttgcgta aagatgtccg cactccccat
    10681 gaagaagccg ttttgaacaa tattcaattc atggaatccg ttcatgccaa aagttattct
    10741 tctattttta gtacactgaa tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat
    10801 acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg aaatctacaa acacggtaca
    10861 ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt
    10921 tatactcctc tttattattt aggaaacaac aaattggcta acgtagcaga gatcatcaaa
    10981 ctaatcatcc gagatgaatc tgtacatgga acctacatcg gttacaaatt ccaattagga
    11041 ttcaacgagt tacctgaagc agaacaagaa acgttaaaag actggatgta caatcttctt
    11101 tatgagcttt acgaaaacga agaacgttat acagaagaat tatacgatcc aatcggctgg
    11161 acagaagaag tcaagacatt cctacgctat aatgccaaca aagcattgat gaacctaggg
    11221 atggatccac tctttccaga tactgccaat gatgtcaacc caatcgtaat gaacgggatt
    11281 tcaacaggca caagcaacca tgacttcttc tcacaagtcg gaaacggcta tttgttaggt
    11341 catgtagaag cgatgaaaga cgacgattat ttgattggat tggaataagg atttatttta
    11401 attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac agatatttta aatgtacact
    11461 gaactctttt ttatgcttat tcacttgttt cttccagcac ttttggactg tctaactttt
    11521 ccgaaacatg ttccattctt ctcaataaat agattttccg gatatttaat attatcgtct
    11581 taatcactgc atagacaggt atgcaaatca gcatgcctaa tattcctgcg acacttcctg
    11641 ctccaatcaa taccaagatg atcgtcaaag gatggatatt cattgatttc ccaaataaca
    11701 acggcttgat caaatttcca tctaattgtt ggatcactaa aatcgacaac gccatataca
    11761 aagcttgaaa tggagagata aacaaaccga caatgattgc tggtaccgct ccaataaaag
    11821 ggccgacata cgggataata ttcgtgattc cgcaaatgac cgcaagcaac aagctataag
    11881 gctgatcgaa tattctcatt gccaaaaagc tcattacacc aataatcaaa gcatctaaga
    11941 tcgtactgct gatataggca gataatgttt cgtttaattc ttttaccgtt tggcgtaact
    12001 ctttacgaat cgctttcgga aagaagtgag acaacgcatc aataaatttg tgcccatcct
    12061 tgaacataaa tagcaaaata aatggaacgg taaataacaa aataaaaaat tgcatcataa
    12121 atgaaatgat ttttgagaca cttgcagata aactgactaa tatgatattg aacaaattac
    12181 taagagataa atttgctgat gctaattgtt cttccaaatt gaaattttta aactcttccc
    12241 gctgcgccaa ttcatttaac catttgcttg tttcatctgc atacattggc aagctctggg
    12301 tcaattgaat tgtctgttcc attaactgag gcactacatt catgacagtt aaaacaacta
    12361 aaatgattac gaccaaaaac gtcaataaaa aaccgaacac tctcgggatc cgatgctttt
    12421 cgagaaaaac aacgattgga tcgaacatat aatagataaa accagcaaca agcaatggta
    12481 aaaaaattgc cgaaagcatt ttcaatattg gactaaaaat attcggcatt tgtaaaataa
    12541 agtacacgcc gatgatcgtg agtactagct ccactgtcca gaaaaataat tttgattgtt
    12601 taaaacgtga ttgcatacat ctcctccaag gtcctttgat acttctattc tatcatttaa
    12661 caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt
    12721 ttaatgagta gacgagtact taaacaaaaa cacggtttgg agtagtgaca aaaattatac
    12781 tgcagtccct ttttccaaat aaacggtgtt caaaagcttg catcttcgta ataagcccag
    12841 ccaaacgaaa aatataagga actattttta tttggctggg cttattactt gatgtaggac
    12901 atctatttta caacctcaga tcatttattg cgcaatactg actaaatgca actttgtgat
    12961 atttttgtgt acggacaact cctcgatcac atcggcataa gtgagccctt ttggaagatc
    13021 aatcgtatag atatttttgt acactcggta gtcatcaacg aattttacgt caaaattcac
    13081 atcttcgatt acgatatttt tctcttcaaa ataatcggta agaaaatctt tggtttcttt
    13141 tctatggata aattggattt ctaacttttt agtcgtaggt acatggatca cgcgtttcac
    13201 tactgtgagt gcaatagcaa tcccgataaa actgatgatc gcaatggaat agtaacccat
    13261 gccgatggcg atccctaatc cagcaattgc ccataaagaa gcggcagtcg taagtcccgt
    13321 caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag aaaccgacac cactcaccac
    13381 ttgagcaatc agccttgctt catctgaacg aatcgttcca gcaagctgtg gatactctaa
    13441 agcatctctt aatgcaccac tagcgatctc aacttggatc aatgcaatga tacatgctcc
    13501 catacaaact aaaatatgcg tacgcatacc tgctggacga tttttatatt gtcgttcaaa
    13561 accgattgct ccgccaaata gcatagcgat ccctaaacgt aaaataatct ctggaattgt
    13621 caataccata tcattcatct aaacaccctc cagtccaata tatcatgtct gaaaggagca
    13681 gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga ctgtgacaaa atttttgtca
    13741 tagtcctttt tatgaataaa cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac
    13801 ggcaaaatat ggggagctat ttttcctttg tcttctctaa tactcaggtc aaaacgctga
    13861 tttcacaacc tctttagcct actattgaaa attacaactt acttgctgct tcttcatcga
    13921 tgatcacaac aacattgtcg tgattttgca atacacttgc tggcaaatga ttcgttacag
    13981 gtccttcaat catccctttg attgcatcag ctttgctttc gccaaatgcc atcaagacga
    14041 tttctttact tttcataatt gaaccgattc ccattgaaac agcttttgtt ggtacatctt
    14101 ctgctttatc gaagaatcgt ttgtttgcat tgattgttga ttctgttaag tcaactactg
    14161 aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc gatatgtcca ttttgcccga
    14221 ttcctaagat ttggatatcg attggatgtg catcgatgat ttgatcatac tttttgcatg
    14281 ctgcttccag atcttctgct tttccatctg gtacaaacgt ttctttgaat ggttttttat
    14341 caaataattg gacatccatg aaatgacggt agctttgttc atctgaaccg cctagtccaa
    14401 cgtactcatc taagtttaca gaagtcattt cactgaaatc caaatcgctt gaagtcattt
    14461 ctttatataa agtaactggt gtgcttcctg ttgccaatcc taatactttt gcacctttgt
    14521 tcataccttc tttgatgatt tcaaatgctt tttttccgcc ttcttcagcg ttttttacac
    14581 gaatgatttc catagtgatt gctcctttat attttggtat agcccaatta tagcattctt
    14641 tttttctttt tacaataatt agtatagacc aaattgcatt tttttcattt tcttgacaat
    14701 aaataggaat ccgtgttagg tttcataaga tggttcaatc aaacaatcca tgttgcatga
    14761 aaaccaagca agtgattcac tgtggacgct ttatgtctgc agttttttta tgaaaaagga
    14821 gttattatga gtatactgac attaaacaac attacccagc aatttggcga caaaatcctc
    14881 tatgaaggtg tcgatctgca attaaatgct ggcgaacatc ttgggctgat tggacaaaac
    14941 ggagccggta aaagtacatt gattaaaatc atcactggtg agatcttacc agatgacgga
    15001 cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg atcaatatgt ggaagtcgat
    15061 gaaacattaa caatcgaaga atttctaaaa actgcgtacg caaaagaata cgaaaaggaa
    15121 gaaaaaattg cttttcttta ccaaacatat agcgagacct tcgatgattc cttgctggaa
    15181 aaagctggga aactccaaac ggaattagac caaggagtgt tttatcaaat cgacacctta
    15241 gttgctgaga tgagcagcgg gttggggatc gatgtcttag gtttagattc actgttgaaa
    15301 aatctaagtg gtggacaacg ttcaaaggtg attttagcaa aactactttt agaaaaccca
    15361 gatgttctga tcttagatga accaaccaat cacttagatg atcagcatat ccaatggctg
    15421 acgcagtttc ttcaggattt tgacggtgcg tatatcgtga tttcccatga ccaagaattt
    15481 cttgatcaga tcaccaccca tatcgcagac atcgagtttg gaaagattac taagtatact
    15541 ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa
    15601 tatcatgctc aacaaaaaca aatcgaaaag accgaagctt atattcgaaa atacaaagcc
    15661 ggttcccgat caacgatggc aaaaagccga caaaaacaat tagataaaat cgaacggctg
    15721 acgcctccag cttcagcaag taaagctgtc tttgattttc cattctcgcc tattgtcacg
    15781 acattagcag tggaaacaac tgatttagtc atcggatatg atcgaccgct acttgcaccc
    15841 atcaatttga ccatccgctt tggtgaaaat attgccattc gaggcttcaa cgggattgga
    15901 aaatctactt tattaaaaac actgatcggt gagattgaaa aaataagtgg cgatttccac
    15961 ttcccagata acacaaaaat caattacttt tcacaagatc tgtactggga aaatccatta
    16021 gaaacaccac tacaatattt aagcagtcaa tttccaaaag cgactatcaa agaattacgt
    16081 cgccaactag ctaaagcagg gttagttaat caattagcaa gtgaacctct cactacatta
    16141 agtggtgggg aacaaactaa ggtgaaactt gcacagttga caatgaacca aggtaattta
    16201 ctgattctcg atgaaccgac gaaccatatc gatcaagaaa cgaaagaaag cttacaagac
    16261 gggatacaga aatttccagg aacagtaatc atcgtctctc atgaacaaga attttatcaa
    16321 gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa cgtttgattg aatagttgaa
    16381 aaaaaggagc tgtgacaaaa gttttgtcac aactccttct tctgaataaa tggtggccaa
    16441 ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta tttttatttg
    16501 gctattctta ttacttgatg cagaacagct tttcacaacc tcttttcgtc tattttttat
    16561 tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa
    16621 aaacaagccc gctaaccgtt acacctaacc agccatactg ctgccacatt gtcgtcccta
    16681 gcaatgaacc agccgcacct ccaataaagt acataaacat aaataca
//
LOCUS       DAJSVF010000055        16489 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSVF010000055 DAJSVF010000000
VERSION     DAJSVF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.057"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..546
                     /locus_tag="ON550_001830"
     CDS             <1..546
                     /locus_tag="ON550_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR3954914.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="ON550_001831"
     CDS             735..1403
                     /locus_tag="ON550_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR3954915.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="ON550_001832"
     CDS             complement(1603..1833)
                     /locus_tag="ON550_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954916.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="ON550_001833"
     CDS             complement(1960..3342)
                     /locus_tag="ON550_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3954917.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="ON550_001834"
     CDS             complement(3498..4685)
                     /locus_tag="ON550_001834"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR3954918.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="ON550_001835"
     CDS             complement(4692..5723)
                     /locus_tag="ON550_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3954919.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5739..7193)
                     /locus_tag="ON550_001836"
     CDS             complement(5739..7193)
                     /locus_tag="ON550_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3954920.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            7368..8273
                     /locus_tag="ON550_001837"
     CDS             7368..8273
                     /locus_tag="ON550_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3954921.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="ON550_001838"
     CDS             8364..9041
                     /locus_tag="ON550_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3954922.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9038..9208
                     /locus_tag="ON550_001839"
     CDS             9038..9208
                     /locus_tag="ON550_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954923.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9322..10218
                     /locus_tag="ON550_001840"
     CDS             9322..10218
                     /locus_tag="ON550_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3954924.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="ON550_001841"
     CDS             complement(10273..11643)
                     /locus_tag="ON550_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3954925.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="ON550_001842"
     CDS             11881..12627
                     /locus_tag="ON550_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3954926.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="ON550_001843"
     CDS             12624..13463
                     /locus_tag="ON550_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3954927.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="ON550_001844"
     CDS             13464..14414
                     /locus_tag="ON550_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3954928.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="ON550_001845"
     CDS             14555..15307
                     /locus_tag="ON550_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954929.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="ON550_001846"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="ON550_001846"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR3954930.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5362 a         3084 c         3317 g         4726 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgctaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg atctactgca
     5761 tcgtgaagtt catcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaa
//
LOCUS       DAJSVF010000056        16121 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSVF010000056 DAJSVF010000000
VERSION     DAJSVF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.059"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            28..1626
                     /locus_tag="ON550_001847"
     CDS             28..1626
                     /locus_tag="ON550_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954931.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLGYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATALTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPENAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ON550_001848"
     CDS             complement(1771..2511)
                     /gene="truA"
                     /locus_tag="ON550_001848"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3954932.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEVPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2702..3337)
                     /locus_tag="ON550_001849"
     CDS             complement(2702..3337)
                     /locus_tag="ON550_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3954933.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="ON550_001850"
     CDS             complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="ON550_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR3954934.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4923..7952
                     /locus_tag="ON550_001851"
     CDS             4923..7952
                     /locus_tag="ON550_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR3954935.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGTNLIPLYMGYQYQKIARLKVLHYLSRFAFIFLTA
                     TLACNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFIYY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVGAF
                     FYCFIFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKANEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYSNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8012..8722)
                     /locus_tag="ON550_001852"
     CDS             complement(8012..8722)
                     /locus_tag="ON550_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3954936.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATIATTTTTS
                     TESKPTAASTETADTSTSSESSKATETTQTSTSSSSVEASSSESSEQVEQILWNADKS
                     AQLEAFMATWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSE
                     SGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNET
                     ANNELKNGFNQIVYGQAN"
     gene            complement(8949..9491)
                     /locus_tag="ON550_001853"
     CDS             complement(8949..9491)
                     /locus_tag="ON550_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3954937.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIDNEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNMFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9496..9723)
                     /locus_tag="ON550_001854"
     CDS             complement(9496..9723)
                     /locus_tag="ON550_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3954938.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(9802..11682)
                     /locus_tag="ON550_001855"
     CDS             complement(9802..11682)
                     /locus_tag="ON550_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3954939.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYMTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLVALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFAKIDTFVFDKTGTLTKGTTAVSTIKNYTENE
                     GDSLALVARLETLSDHPLGRAVIHYVQEKDIDFQQLAVKDNQTIKGQGITAEIDGHAV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSFVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11872..12486)
                     /locus_tag="ON550_001856"
     CDS             complement(11872..12486)
                     /locus_tag="ON550_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3954940.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMGNELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKIKGIGTQLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12628..13188
                     /locus_tag="ON550_001857"
     CDS             12628..13188
                     /locus_tag="ON550_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3954941.1"
                     /translation="MKKTDRRVKKTEKALAEALSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13493..13807)
                     /locus_tag="ON550_001858"
                     /pseudo
     CDS             complement(13493..13807)
                     /locus_tag="ON550_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14061..15701)
                     /locus_tag="ON550_001859"
     CDS             complement(14061..15701)
                     /locus_tag="ON550_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954942.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKRFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITTIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIVNSEGLVSIEGTQGETIIEV
                     SSIYPPTMKLAFLISLCSYLFLLISFLIRKLQHIKIMI"
BASE COUNT         5096 a         3095 c         2523 g         5407 t
ORIGIN      
        1 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
       61 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
      121 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
      181 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
      241 tataaaatga tgggaaatct tgttttaggt tataatatct actatttttt tatcactttt
      301 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
      361 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
      421 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
      481 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
      541 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
      601 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
      661 gccctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
      721 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
      781 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
      841 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
      901 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
      961 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     1021 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     1081 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     1141 catgctaaca aagattcatt aacacttata gcacctgaaa acgctgttgc tatagctgaa
     1201 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     1261 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     1321 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     1381 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     1441 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     1501 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
     1561 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
     1621 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
     1681 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
     1741 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
     1801 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     1861 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     1921 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     1981 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     2041 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     2101 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     2161 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     2221 aggcacttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     2281 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     2341 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     2401 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     2461 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     2521 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     2581 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     2641 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     2701 tttactgttt gagatgcaag atactcaacc aggcaatggg cacataatta aagaaaaaac
     2761 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     2821 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     2881 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     2941 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     3001 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     3061 acgcgatggc tgctgtaact agggtagcta atacacgaga tgtacttaca aatgcagcat
     3121 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     3181 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     3241 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     3301 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     3361 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     3421 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     3481 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     3541 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     3601 agtttccatt gaatcctgga taacaccgat aataaatcca ataccaacga tttgcatcgc
     3661 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     3721 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     3781 aacaaatggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     3841 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     3901 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     3961 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     4021 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     4081 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     4141 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     4201 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     4261 tgctcgaaaa gctagtccca acaatacagc ccagaataaa acgccaaggt aattcgcatc
     4321 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     4381 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     4441 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     4501 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     4561 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     4621 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     4681 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     4741 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     4801 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     4861 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     4921 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     4981 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     5041 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     5101 ttttacaaac ttccgttgtt attggtacaa atttgatccc actttacatg ggttaccaat
     5161 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     5221 taacagccac actagcttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     5281 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5341 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5401 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5461 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     5521 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     5581 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     5641 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     5701 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     5761 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     5821 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5881 ggaaggcatg gctgataaat atcgggcagt tttttattta ctatttatta gcagtgtccc
     5941 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6001 acttcgcttt tgattcgatt gaacaattaa cacaacgttg gaaaaaaaga attcattggc
     6061 tttttgttca taaaagttat tttttagttg gagcattttt ttactgcttt atcttcgtgc
     6121 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6181 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6241 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6301 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6361 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     6421 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6481 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     6541 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcataaaaat tcgccttctt
     6601 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     6661 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     6721 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcaa
     6781 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6841 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6901 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     6961 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7021 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7081 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7141 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7201 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7261 aagaaacctt gaaagcttta caaagcaata cagaaacgtc gcaattcatc caattatcca
     7321 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7381 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7441 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7501 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     7561 atggattaga acagcaccag acagattatt tcatttatag caacaagtat agtcgtgaac
     7621 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     7681 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     7741 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     7801 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7861 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7921 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     7981 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     8041 taaagccatt tttcaattca ttattcgctg tctcgttaaa gtaaagataa ttatcgggat
     8101 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     8161 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     8221 aatctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     8281 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     8341 tcatttgatt cgtatagctt ttgtattgtt gtcccattgt ttggccccat gtagccataa
     8401 atgcctctaa ttgcgcggat ttgtctgcat tccacagtat ttgttcgact tgttcgctgc
     8461 tttctgaaga tgaagcctct acagaagaac ttgaagtaga tgtttgagtt gtttcagtcg
     8521 ctttggaaga ctctgacgaa gtagaggtat ctgctgtttc cgtacttgct gcagttggct
     8581 tgctctctgt gctagttgtt gtagtagttg caattgttgc tttcgctgtt tcttttgttg
     8641 attctttttt cgattcattc ccgttggtac aaccagctaa caaaaaagct agaccaattg
     8701 ttactaaaat cctttttttc aattaaaaaa ctcctttcga ttactcccac ttctttattc
     8761 tatcaaataa gcaatgtgat tttattgatt ttttgtattt tctcatccat cttttaaacc
     8821 ttttaaaaac taagaagcac ccgctgatgt tgtctgtaat acatcagcgg gtgcttcttt
     8881 tattttaatc ttcgtgattt tccaatttac ctcacgcttt atatttgtat ttgaagaaac
     8941 atagactttt agtcatcatc gtcttcctca tccaaatctt gtcggacagt ttttccttgg
     9001 agtctttgta cttggtagat gatttcttta ttgaaactta aaagttcaac gagtaattgt
     9061 tgcaagccat atttttcttc tttttcagct aacttgattg cccgtgtgat aaacatattt
     9121 tggaaatcaa aatcttttgc aaagttttcc aacattgttg aggctggtaa atatttgttt
     9181 tgaccatgtt cgtaaatttt tccataacgc atgacttctt ctaaagtaga cggaacgatt
     9241 tcattatcaa tcactaactc atgagagatt ttattgaacc atctcgcttc ttgatcagca
     9301 tattctgaaa aaagggtact taattcttgc gaagatgtac ccaaaacata caattgtgct
     9361 tggatcaatt tcatttcttc taatttaaaa tttgctaata catgccccac catggcgcca
     9421 gcagttggtt tatgatggtc gatatctgct tgttttgttt cttgagcaaa tttttcttcc
     9481 actatattca ctgatttacg cctcttttac ttttgttgat tggacttcat aacctaattt
     9541 ttccacaact gcagctaatt gttcagggtg gacttcatct ggatcaaagt ttgctttgat
     9601 tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca ggctgttgag aaaccgcatg
     9661 ttcgattttt tgcatacagc ttggacaagc tagtgtatct aattgcataa taattttttt
     9721 catcgtagtt cttccttcct ttttcttgat agtcttattc tagattacag cttccataaa
     9781 aaaattgatt cttatcaagt tttacgattt ttcttctttg ctttcgtgtc caaaatacaa
     9841 taaacgcatc gcgttaaaga taacgatcaa gatactagct tcgtgaacga acatcccact
     9901 agccatgtaa ataaacccgg caaataagcc gatgagtaaa aataagacga caccaatggc
     9961 aatcactaca ttttcacggg tatttaaaac agttttctta gccaagcggt aggcatgaac
    10021 aaacgatcca aaactagatt gcatcaagac aacgtcactc gtttcgatag caacatccgt
    10081 tcctgatccc atagcaatcc cgatatcagc cgcagccaaa gagggagaat cattgattcc
    10141 atctccaata aaagcaacgc gcaatccttg ttcttggtat tttttcacaa attgaacctt
    10201 ttcgtctggt aataattcag catggacttc atcaatgcct aacatatcag ctacataatc
    10261 agcagtcatc tgattatcac ctgtcagcat cactaactgt tttgctcctg cttgtttcaa
    10321 cttagctaat tgttcagcga cttctggtcg aatcacatct gatacaccta tgatttgagt
    10381 gatttttttg tcgattgcta caataatgac tgagctgcct gtttgttgta attgatgtaa
    10441 atcttgtact tgctttggtg taagagaaag tttgtgagcg gcaatcaatt tggtgttgcc
    10501 cgcacaaact gcatgaccat caatctcagc tgtgatgcct tgtcccttga ttgtctgatt
    10561 atctttgact gctaattgtt gaaaatcaat gtctttctcc tgaacataat ggatcacggc
    10621 gcgccccaga ggatgatcgg ataacgtttc taatcgagcc actaaagcta agctgtcccc
    10681 ttcattctct gtatagttct tgatagtgga tacagcagta gttcctttgg tcaatgtacc
    10741 ggttttatca aagacaaaag tatcgatttt ggcaaaagta tccattgctt caccgccttt
    10801 gactaagatg ccccgtttcg ctccattacc gattcctgct acattcgaaa caggcgcacc
    10861 aatgactaaa gcgccaggac aaccaagaac caaaacagtg attgctaaac gaaaatcacg
    10921 actgatcaga ccaacaagca acgcaacaag taaaacggtt ggagtataat actgagaaaa
    10981 tcgatcaatg aatttttccg catgggattt ggtatcttgt gcttcttcta ctaattcgat
    11041 aattttggca aaggtcgtat catctccgac cttagttgct tcgatcgtca tatagccatc
    11101 tgctaaaatc gttccagaga agacttcatc tccactgatt ttgacgactt gtcgtgattc
    11161 accagtcact gcggcttcat ccgcataacc ttttccgtca atgactcgtc cgtccacagg
    11221 aatagctcca ccagttttta ctaagacaat atcgccttct tccacgtcat cgatttccac
    11281 ttcttcagtc gttccatctt cttggacaac actagcagtc gatggggcca tctcggtcaa
    11341 agccttgatt gattcccgcg ttttggtcaa tgttttttgt tccagatagc taccaaacaa
    11401 aaacaagaat gtaacgatag ctgactcctt gtattctccg atcataaagg caccaagcac
    11461 agcaatagta acgagtaaat caattgagat aactttgact ttcactgcct gatacgcatg
    11521 gatcataata ggtatgaccg caataataga agctatggct agagcaatgt tatagaacaa
    11581 gacaaatcca gtaaattttc cgataaaacc aaaaataatc aacacagctg ataaggcggt
    11641 gatatgattt cggtgctctt ggatccactt ttgaaatttc atctttctcc ctcttttcct
    11701 tttcgttctt acgagactaa gtatagagac ttatccatca taaaaaattg accactatca
    11761 agtttagtca aaaaggcggt tttcttttgg tagaatatac tacaaaaaat agttagcaag
    11821 aagactcact aaaagtgatc ttgccaacta ttgtcgtttc tatcaaaatt ttcattgaat
    11881 gattttcctg tacagcatac atcggagatc tatttcttta tctaagattt ttatcttgct
    11941 aattttttcc tttagtcttt caaatccacg tcgttcatag aattgatacg tacagccgct
    12001 atctgtaaaa aggaatattt cttttccttt ctctctcttt tcaaattctg ttagcaactg
    12061 ggtgccgatg ccttttattt tcaaatcagg atttgcagct aaaaaaatga tctctccatc
    12121 tggcgaatgc ttctttaaat atgctgcaag catttgttca tttgctgtct cgtaagctcc
    12181 tttactttct ttagcaaata tattttgtaa aaaatcgaat atcttaacat atatttgctg
    12241 tccaaaagaa cgatgtttct ttttttctcc ttttatctcc gccagcaaaa cacctgccaa
    12301 ctcgtcctct gcatacaaag caagaatttg agttgctcga ttcaactcca tatacaaaaa
    12361 atatcttccg taaagattca gcagaagttc atttcccata tatctctcaa agttcattcc
    12421 ttcaacagca aaatagatag cttttttgta atctttcttt tccaagggtt taaaagttag
    12481 tttcatcatt tcgcctcctc aacttcaatg attgtgttat acttacatta taatagacac
    12541 agtgtctact atctataatc aatcatctat aagattgtcg attaagcgac agctttgctt
    12601 tgatatgtcg gataaggaga taggtaaatg aaaaagacag atagacgcgt caaaaaaaca
    12661 gaaaaagctt tggctgaagc actatccaca ttattagtaa ataaaaagat tcaagcaatc
    12721 actatacgcg aactaacaga aacggcagat gtacatcgtt cgacttttta tacacattat
    12781 aaggatatct atgatctata tgatcaatta gaaagcaact tttttcggga tctaaatgaa
    12841 attctctctt atgatccagc ccatagctat gaggaactat acactcgctt gattgactat
    12901 gtttacgcaa atactttttt atatcgctta tttatcctca accatggtga tatttcgttt
    12961 cagaagaaag ttaaacaagt tattgaagaa aactatctga acatatggct atatgaagac
    13021 aagaaaacga ttatcacaga agaaatgaga ttcttcacta cgtaccacgt ccaaggttgt
    13081 ctatctgttc tcaatcgatg ggtagaaagc aattacaagt actccaaaaa agaattatta
    13141 gaactcttac agaaactgaa tgatcacata gagaaaatca tgccttgaat aaaaacatac
    13201 taagcttagt taagaagaac cgtaaccttc tcgtataaca actgaatctt atttagccaa
    13261 attgtttact gaagattttt taagtaaaat ttctataatt cctacttatt gaaaagtagt
    13321 ttgtagtgct gcttctccct ctctaagtga tatatcttgt tgtaccaatc aaattcacct
    13381 catcattaat aaattacttt taggctaatt tattacaact tataggttga agctgtaacc
    13441 aatttatagt catattatat ttttatcatc cagtttattg aattcttctc gtttacctca
    13501 attcattttc aagtggaatc agctcatcat ctaacatttt gcgccatgct tgcttcattt
    13561 cttgtgggta tttaaatggc ttcgcttctg gatgtttagc tttatatcct tcgtaatcct
    13621 tactgttgat aattgctttc atattaggaa ttggatcttc acctggcata tattgcagat
    13681 tagaaacatg taattttttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
    13741 catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttgctatcta
    13801 gtgtaccttc atttctttgt gcttagattc aaagataatt ttctcaataa agatttttga
    13861 ttccagtaag attcctttac tccttttaca acatagcgtt gtcctttgtt ttgaaacatc
    13921 ttcttcccct ctttgccatt aaaaatagcc caattctcta taaagaagat taagcttcat
    13981 tattataata taaagtttaa atttggttta ttacttactt tttcttattc tctagtaagc
    14041 taatattgaa aaaaatgtaa tcagatcatt atttttatat gttgtaactt cctgataaga
    14101 aaactaatta aaagaaagag gtaactacat aaactaatta aaaaagctaa cttcatagtt
    14161 ggaggataaa tggaagaaac ttcaatgatt gtttctcctt gtgttccttc aatcgacact
    14221 agtccttcac tattaacaat ctctcttttt tgtccattta cttttgcgac tgtcccccaa
    14281 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
    14341 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
    14401 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
    14461 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
    14521 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
    14581 acaattgtcg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
    14641 aacgataaaa tcaacgagcc aaagagtact tgaaatccta agattcgcca aggaaattga
    14701 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
    14761 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatct tttaaaaaag
    14821 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
    14881 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
    14941 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtaa
    15001 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
    15061 tctttttttc ttgtttcact tactacaaag ccacctaaga ctagtaagcc aacggctatt
    15121 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
    15181 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
    15241 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
    15301 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
    15361 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
    15421 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
    15481 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
    15541 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
    15601 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
    15661 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
    15721 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
    15781 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
    15841 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
    15901 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
    15961 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    16021 gtctcactta tgataaggcg ctgcgtatca tgagtgaatg atattaggaa aacttcataa
    16081 aataaatctt ttatacattt ccacttctag atggttattc a
//
LOCUS       DAJSVF010000057        15391 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSVF010000057 DAJSVF010000000
VERSION     DAJSVF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.060"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..541)
                     /locus_tag="ON550_001860"
     CDS             complement(<1..541)
                     /locus_tag="ON550_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3954943.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNAS"
     gene            complement(627..1586)
                     /locus_tag="ON550_001861"
     CDS             complement(627..1586)
                     /locus_tag="ON550_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3954944.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(1598..3109)
                     /locus_tag="ON550_001862"
     CDS             complement(1598..3109)
                     /locus_tag="ON550_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3954945.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(3131..3694)
                     /locus_tag="ON550_001863"
     CDS             complement(3131..3694)
                     /locus_tag="ON550_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3954946.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(3700..4563)
                     /locus_tag="ON550_001864"
     CDS             complement(3700..4563)
                     /locus_tag="ON550_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3954947.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(4577..5473)
                     /locus_tag="ON550_001865"
     CDS             complement(4577..5473)
                     /locus_tag="ON550_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3954948.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(5648..6937)
                     /locus_tag="ON550_001866"
     CDS             complement(5648..6937)
                     /locus_tag="ON550_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3954949.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(6909..8426)
                     /locus_tag="ON550_001867"
     CDS             complement(6909..8426)
                     /locus_tag="ON550_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3954950.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8624..9514
                     /locus_tag="ON550_001868"
     CDS             8624..9514
                     /locus_tag="ON550_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR3954951.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(9587..10411)
                     /gene="nadE"
                     /locus_tag="ON550_001869"
     CDS             complement(9587..10411)
                     /gene="nadE"
                     /locus_tag="ON550_001869"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR3954952.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(10423..11895)
                     /locus_tag="ON550_001870"
     CDS             complement(10423..11895)
                     /locus_tag="ON550_001870"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR3954953.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(12532..12894)
                     /locus_tag="ON550_001871"
     CDS             complement(12532..12894)
                     /locus_tag="ON550_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3954954.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            13117..13869
                     /locus_tag="ON550_001872"
     CDS             13117..13869
                     /locus_tag="ON550_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3954955.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(14009..14932)
                     /locus_tag="ON550_001873"
     CDS             complement(14009..14932)
                     /locus_tag="ON550_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3954956.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(14947..15219)
                     /locus_tag="ON550_001874"
     CDS             complement(14947..15219)
                     /locus_tag="ON550_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954957.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         4741 a         3156 c         2487 g         5007 t
ORIGIN      
        1 aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc
       61 tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga
      121 tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat
      181 cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc
      241 ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt
      301 ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc
      361 ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat
      421 gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt
      481 cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca
      541 tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt
      601 caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct
      661 ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct
      721 aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac
      781 ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa
      841 gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc
      901 tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta
      961 atgaatattt tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt
     1021 ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga
     1081 ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc
     1141 cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc
     1201 cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga
     1261 ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt
     1321 gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat
     1381 tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat
     1441 tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta
     1501 aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata
     1561 ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta
     1621 cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat
     1681 agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc
     1741 tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta
     1801 tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta
     1861 cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa
     1921 tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc atcacataat
     1981 aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta
     2041 ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta
     2101 atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga
     2161 cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta
     2221 gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt
     2281 cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct
     2341 catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag
     2401 ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg
     2461 atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact
     2521 tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca
     2581 cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt
     2641 tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt
     2701 ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt
     2761 tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta
     2821 tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca
     2881 cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt
     2941 cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg
     3001 gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc
     3061 gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca
     3121 acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc
     3181 caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata
     3241 acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt
     3301 caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa
     3361 gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt
     3421 cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt
     3481 tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct
     3541 cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa
     3601 caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca
     3661 actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca ttccagacat
     3721 agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat
     3781 tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa
     3841 agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag
     3901 caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct
     3961 caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc
     4021 ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc
     4081 agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac
     4141 ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa
     4201 atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga
     4261 atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta
     4321 atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc
     4381 aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag
     4441 ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat
     4501 ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc
     4561 catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg
     4621 agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca
     4681 attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa
     4741 attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca
     4801 gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa
     4861 actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc
     4921 cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga
     4981 tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga
     5041 cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa
     5101 tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat
     5161 tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg
     5221 gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac
     5281 taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca
     5341 aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa
     5401 ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg
     5461 tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt
     5521 aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg
     5581 caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag
     5641 ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct
     5701 caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg
     5761 cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt
     5821 cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag
     5881 gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa
     5941 actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc
     6001 catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat
     6061 aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta
     6121 gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct
     6181 tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa
     6241 attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat
     6301 ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag
     6361 ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta
     6421 atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg
     6481 ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag
     6541 atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta
     6601 catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa
     6661 tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga
     6721 tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag
     6781 atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg
     6841 aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta
     6901 accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca
     6961 ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt
     7021 gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca
     7081 ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga
     7141 ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg
     7201 gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga
     7261 atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag
     7321 atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacatttacc
     7381 aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga
     7441 cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc
     7501 tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca
     7561 aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct
     7621 gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca
     7681 tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga
     7741 tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat
     7801 aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac
     7861 cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct
     7921 aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc
     7981 caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca
     8041 cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca ccagttataa
     8101 aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta
     8161 ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca
     8221 ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga
     8281 aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt
     8341 cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca
     8401 ctactaatca acattgtggc ttccataact gacctccttt aatttataca tgaataatac
     8461 acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt
     8521 taattaacca aaatattaat aatgattaaa acaaataatt taatataact tttattaaca
     8581 aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac tacaaataaa
     8641 cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca agatcatcca
     8701 gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg gagtgagcag
     8761 tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca aaacagaaaa
     8821 agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca gttttccaga
     8881 aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc actatacaaa
     8941 ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg ggaaagtgga
     9001 tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct ggtttaccag
     9061 cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat tggagatgtt
     9121 ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt ggccttcaga
     9181 tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg gagattttgc
     9241 tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc aatacggctt
     9301 gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac ccttgtcaaa
     9361 catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa tcgaagtgaa
     9421 agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg gtaattacga
     9481 tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat tttataaaac
     9541 aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat ttccagaaat
     9601 catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac catgcttcga
     9661 ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca atttcattat
     9721 atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca agatctgcag
     9781 tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat tgtttccctt
     9841 gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca aatttcgtga
     9901 agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct gctctttctc
     9961 cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct ttgttaaaat
    10021 cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca gctggtttga
    10081 tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt gcatcttctt
    10141 catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt tcatcttttg
    10201 tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt
    10261 ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc aaatatgctt
    10321 tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt ttgacaccta
    10381 attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt ctgtatgaag
    10441 ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc agcattcagt
    10501 agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt cccatagtga
    10561 atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt cgtaaactct
    10621 ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt
    10681 attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc gagggtcttc
    10741 atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag tgatgcgcca
    10801 cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact tgatcgtatc
    10861 catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac caagggctgg
    10921 ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt tagctttttg
    10981 catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga tttttgcttc
    11041 cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg ccatatctcc
    11101 actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg ccacgcgaat
    11161 cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt
    11221 atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt ggactaacga
    11281 atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta cattgcttgt
    11341 tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat ccagttcttg
    11401 tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt tgatacgagc
    11461 tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg tttctactaa
    11521 ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa aaactagttc
    11581 tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa
    11641 accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat tgaacgtcag
    11701 attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa
    11761 aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac ctaattccca
    11821 gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg
    11881 gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct acccattata
    11941 acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca catgttttac
    12001 ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt attggtattt
    12061 aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga agaaattagc
    12121 gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat cgtagccgtt
    12181 aagataccga tgattgaaca aatataacca aatgtctcta caacataatc aatggcaaaa
    12241 gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag aaacggatat
    12301 aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat accttgcgtt
    12361 gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta agaaagcagc
    12421 tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt gttttttatt
    12481 gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt attaaataga
    12541 atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt tgaattgtat
    12601 tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt ctcgtgtcat
    12661 ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt tcttcgtatt
    12721 gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg ttcgatctaa
    12781 cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca ctactttagg
    12841 ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct tcatctcatt
    12901 cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat ctttcctagc
    12961 attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt ttatcactta
    13021 atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt
    13081 aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat aaagaaaatg
    13141 tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat tggactgcta
    13201 aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt
    13261 ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat ccaagaggtc
    13321 ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca aatgttgctg
    13381 gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact tgatatgtac
    13441 gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg
    13501 aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa aaaaaaggtt
    13561 acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga aatgcaaact
    13621 gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga agatgaaagc
    13681 ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg gcctgaatat
    13741 cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg gttcactgca
    13801 tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa
    13861 aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc taataacaaa
    13921 acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc attctttcac
    13981 aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa ggtattttca
    14041 ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa gcttccagat
    14101 ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg cgtccaatca
    14161 ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc atatccgaat
    14221 ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt tttcctttct
    14281 ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa agttgatgac
    14341 atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc aacgtgatgc
    14401 ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt gattgttctc
    14461 gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa actagaggtc
    14521 catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta atgatcaatc
    14581 catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct ttatgatagc
    14641 ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg atcccactga
    14701 tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg cttttcgttt
    14761 ttccataact taactgtctg gcatttgcat tagggatata atcgacttct ttcatgaccg
    14821 caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt acacgtgaaa
    14881 tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc actgctgctt
    14941 cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac cgcattcaaa
    15001 aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc aatatatatc
    15061 ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc tattcaaccc
    15121 ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat aaaaataatt
    15181 gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc cttttgatac
    15241 tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt
    15301 tttattcctc tttcattatt accaaagtct cttattttaa attgaataca ttataatgat
    15361 actgtaaata tatctttatt attagtgaat g
//
LOCUS       DAJSVF010000058        15314 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSVF010000058 DAJSVF010000000
VERSION     DAJSVF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.061"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            278..787
                     /locus_tag="ON550_001875"
     CDS             278..787
                     /locus_tag="ON550_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3954958.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ON550_001876"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ON550_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3954959.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ON550_001877"
     CDS             2322..3302
                     /locus_tag="ON550_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3954960.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ON550_001878"
     CDS             3424..4842
                     /locus_tag="ON550_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR3954961.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ON550_001879"
     CDS             4913..6382
                     /locus_tag="ON550_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3954962.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ON550_001880"
     CDS             6392..6859
                     /locus_tag="ON550_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3954963.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="ON550_001881"
     CDS             6895..7767
                     /locus_tag="ON550_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3954964.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ON550_001882"
     CDS             7849..10383
                     /locus_tag="ON550_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3954965.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ON550_001883"
     CDS             10539..11312
                     /locus_tag="ON550_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3954966.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ON550_001884"
     CDS             11305..12096
                     /locus_tag="ON550_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3954967.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ON550_001885"
     CDS             12106..12597
                     /locus_tag="ON550_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3954968.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ON550_001886"
     CDS             12615..13025
                     /locus_tag="ON550_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3954969.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ON550_001887"
     CDS             13029..14429
                     /locus_tag="ON550_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR3954970.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ON550_001888"
     CDS             14452..14826
                     /locus_tag="ON550_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3954971.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DAJSVF010000059        15293 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSVF010000059 DAJSVF010000000
VERSION     DAJSVF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.062"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(95..2194)
                     /locus_tag="ON550_001889"
     CDS             complement(95..2194)
                     /locus_tag="ON550_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3954972.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(2414..3523)
                     /gene="rpoD"
                     /locus_tag="ON550_001890"
     CDS             complement(2414..3523)
                     /gene="rpoD"
                     /locus_tag="ON550_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR3954973.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(3547..5388)
                     /locus_tag="ON550_001891"
     CDS             complement(3547..5388)
                     /locus_tag="ON550_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3954974.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(5570..7885)
                     /locus_tag="ON550_001892"
     CDS             complement(5570..7885)
                     /locus_tag="ON550_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3954975.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(8070..8930)
                     /locus_tag="ON550_001893"
     CDS             complement(8070..8930)
                     /locus_tag="ON550_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR3954976.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(8989..9619)
                     /locus_tag="ON550_001894"
                     /pseudo
     CDS             complement(8989..9619)
                     /locus_tag="ON550_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            9786..10547
                     /locus_tag="ON550_001895"
     CDS             9786..10547
                     /locus_tag="ON550_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3954977.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            10573..13164
                     /gene="mprF"
                     /locus_tag="ON550_001896"
     CDS             10573..13164
                     /gene="mprF"
                     /locus_tag="ON550_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3954978.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(13352..14539)
                     /gene="tuf"
                     /locus_tag="ON550_001897"
     CDS             complement(13352..14539)
                     /gene="tuf"
                     /locus_tag="ON550_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3954979.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            14907..15263
                     /locus_tag="ON550_001898"
     CDS             14907..15263
                     /locus_tag="ON550_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3954980.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4186 a         3184 c         2706 g         5217 t
ORIGIN      
        1 aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa
       61 cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc
      121 atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt
      181 tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc ccattttcga
      241 ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag
      301 caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa
      361 tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg
      421 aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg
      481 cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg
      541 cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt
      601 tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct actgcttctt
      661 ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta
      721 tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg
      781 cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat
      841 ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc
      901 actcattcac tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta
      961 actctgaata tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat
     1021 cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa
     1081 gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt
     1141 ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg
     1201 ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt
     1261 tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg
     1321 cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag
     1381 taccctcttt gcttacagat tgattttcag catcggattc actatttatt tcatcgggct
     1441 ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat
     1501 tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg
     1561 tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt
     1621 tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga
     1681 tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat
     1741 aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc
     1801 ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct
     1861 catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag
     1921 gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta
     1981 aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat
     2041 cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta
     2101 tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca
     2161 tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag
     2221 gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg
     2281 atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt gagactgcgt
     2341 agtctcaagc tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt
     2401 caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca
     2461 attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt
     2521 tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga
     2581 cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact
     2641 cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt
     2701 gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca
     2761 agatttctcg gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag
     2821 gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt
     2881 caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg
     2941 cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt
     3001 tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc
     3061 ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac
     3121 gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg
     3181 cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt
     3241 ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac
     3301 tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt
     3361 caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct
     3421 gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg
     3481 ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt
     3541 gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc
     3601 aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt
     3661 tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg
     3721 cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc
     3781 atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc
     3841 atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt
     3901 taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt
     3961 ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg
     4021 ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg
     4081 tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc
     4141 ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat
     4201 cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa
     4261 gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag
     4321 atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt
     4381 ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata
     4441 gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact
     4501 tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat
     4561 aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag
     4621 attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg
     4681 ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg
     4741 gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc
     4801 atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt
     4861 cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc
     4921 aattaattct aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt
     4981 tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt
     5041 ccccgtagca gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa
     5101 cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag
     5161 ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg
     5221 cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa
     5281 gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt
     5341 ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc
     5401 tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa
     5461 atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat
     5521 catatttcat tgctttttca agtataacac atttctatct cattaagcat caatttgcta
     5581 ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga agaacaatat
     5641 ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca
     5701 aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga
     5761 agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa
     5821 tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa
     5881 gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa
     5941 gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga
     6001 aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa
     6061 atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa
     6121 aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt
     6181 ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc
     6241 catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca
     6301 caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt
     6361 tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt
     6421 ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac
     6481 gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac
     6541 cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt
     6601 caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag
     6661 cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa
     6721 gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa
     6781 attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc
     6841 gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt
     6901 ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt
     6961 ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg
     7021 tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg
     7081 ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa
     7141 gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga
     7201 tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt
     7261 tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct
     7321 cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca gccatgacag
     7381 aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat
     7441 cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat
     7501 cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag
     7561 tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg
     7621 ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata acgatattga
     7681 cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc
     7741 caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg
     7801 tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta
     7861 cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa
     7921 taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt
     7981 tatagaaaaa cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc
     8041 cgtgtgctgt ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc
     8101 caccaaccta gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact
     8161 cccaatcccg cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa
     8221 atgaaatgga atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat
     8281 ccaagaacac aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct
     8341 ttaaatccgt cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc
     8401 aatcctggga cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa
     8461 ttctgatcga cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga
     8521 acaaaagtaa caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct
     8581 ttccataacg caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt
     8641 tcatacgcac ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg
     8701 cctagaccaa caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct
     8761 aaaaaagaaa tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact
     8821 cctggcaaga taaatcctga accgataaac attcccttca caaaccgtaa catccagtct
     8881 ttggttgtgt accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca
     8941 ctccttcttt catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac
     9001 tttttcatag gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt
     9061 acctccatta tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg
     9121 gtattgtttc ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc
     9181 tgctaattct actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc
     9241 aaaattatac ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca
     9301 tttccgccaa atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag
     9361 cttctccatg cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg
     9421 tcaatattat cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg
     9481 ttgttatttc tatttgatag ctttccactc gttttaccga acgataagtt cttcctccta
     9541 aaaacaacac cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag
     9601 ataatcgttt tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta
     9661 tttctttatc ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga
     9721 attttcctgt attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt
     9781 gcccaatgac tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa
     9841 agaaatatgc aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct
     9901 tccctctctt tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc
     9961 ctgtactcga acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga
    10021 caaacgaagc atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt
    10081 tgctggaaaa aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac
    10141 tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca
    10201 cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg
    10261 tatggttact ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt
    10321 tgctgcatga catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga
    10381 tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta
    10441 taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc
    10501 ctgaacgctt caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag
    10561 aaggtgattt atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc
    10621 aaaacaatct ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt
    10681 aaaactctat ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc
    10741 ggactaatgc tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt
    10801 ttgaatcgga tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg
    10861 atcaatacga tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct
    10921 gaactatatg gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc
    10981 ttgtttttga tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc
    11041 acgccagttg ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat
    11101 tttcctgtcg tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca
    11161 cttaaaactc aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt
    11221 agtttccttt ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca
    11281 ttattcattg ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt
    11341 ttcgatgtaa tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt
    11401 gtgtggatct tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc
    11461 tttttcttca aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa
    11521 ttagcaacag aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg
    11581 ctagtacttt ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg
    11641 aacccattga aatttcattt tattatccaa tttcctagta tactattggg atttctttta
    11701 atcattatgg gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc
    11761 ttgattgttc tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt
    11821 ttcttgtcta ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa
    11881 ttgatctatt catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg
    11941 ctttacatcg ttattggggt atacaatctg ccagatttcc ctcatcggcg tcaccatttt
    12001 atttcctttt tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc
    12061 gctgttagtt tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt
    12121 ggcgaagcct ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc
    12181 gatagtcaat tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa
    12241 ccaactgttc ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct
    12301 tctggtaaga aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga
    12361 ctatgctatt taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa
    12421 ttcggctacg atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca
    12481 acttcaggga aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga
    12541 tttacatttg atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat
    12601 atctctgata attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct
    12661 gaagattatc tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc
    12721 gcttttgcaa cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg
    12781 cgatatgatt ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt
    12841 gcctatatga aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat
    12901 gtcggaactt ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt
    12961 ggttctcatt tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac
    13021 tgggtctcac gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct
    13081 ttgctgatta ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa
    13141 aaatggatac gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga
    13201 ataaaagaga ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg
    13261 aagaacaaag agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt
    13321 tttcattacc aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg
    13381 aacctactgt ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg
    13441 gatggatcaa gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt
    13501 ctggcaatgt gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt
    13561 tgttgaagaa tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt
    13621 tgaattttgt atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat
    13681 cgtcacgttg gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta
    13741 acgttttacg gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc
    13801 ctacgatctc tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa
    13861 cagttcctcg acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg
    13921 tatcacgttc tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag
    13981 cttcagcatc tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt
    14041 catcgcctgg gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga
    14101 tcaattcttc atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac
    14161 caacttggcg tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag
    14221 aaacaactaa gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt
    14281 ccgcgtgtcc tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt
    14341 gtgcggtatt gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat
    14401 agtcttgagg atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag
    14461 tagttttccc atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt
    14521 cataatgttc ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt
    14581 tcagctctca cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac
    14641 ttactttttg taagattcga actaagctta ttatacgtca atattggtca aagtcaacta
    14701 agagcactcg tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt
    14761 tttgagaaaa acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag
    14821 gaaaagttta aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt
    14881 taataataag gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag
    14941 gcatatttct cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt
    15001 atacaaaaat cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat
    15061 tcacgactta tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga
    15121 cgcttcgcga agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca
    15181 atcctcgtaa aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg
    15241 cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DAJSVF010000060        15070 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSVF010000060 DAJSVF010000000
VERSION     DAJSVF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.063"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(99..476)
                     /locus_tag="ON550_001899"
     CDS             complement(99..476)
                     /locus_tag="ON550_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3954981.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            732..1460
                     /locus_tag="ON550_001900"
     CDS             732..1460
                     /locus_tag="ON550_001900"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR3954982.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1460..1714
                     /gene="purS"
                     /locus_tag="ON550_001901"
     CDS             1460..1714
                     /gene="purS"
                     /locus_tag="ON550_001901"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR3954983.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1716..2387
                     /gene="purQ"
                     /locus_tag="ON550_001902"
     CDS             1716..2387
                     /gene="purQ"
                     /locus_tag="ON550_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR3954984.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2388..4610
                     /gene="purL"
                     /locus_tag="ON550_001903"
     CDS             2388..4610
                     /gene="purL"
                     /locus_tag="ON550_001903"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR3954985.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4595..6034
                     /locus_tag="ON550_001904"
     CDS             4595..6034
                     /locus_tag="ON550_001904"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR3954986.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6057..7109
                     /locus_tag="ON550_001905"
     CDS             6057..7109
                     /locus_tag="ON550_001905"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR3954987.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7106..7684
                     /gene="purN"
                     /locus_tag="ON550_001906"
     CDS             7106..7684
                     /gene="purN"
                     /locus_tag="ON550_001906"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR3954988.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7681..9216
                     /gene="purH"
                     /locus_tag="ON550_001907"
     CDS             7681..9216
                     /gene="purH"
                     /locus_tag="ON550_001907"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR3954989.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9232..10506
                     /gene="purD"
                     /locus_tag="ON550_001908"
     CDS             9232..10506
                     /gene="purD"
                     /locus_tag="ON550_001908"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR3954990.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10628..11542
                     /locus_tag="ON550_001909"
     CDS             10628..11542
                     /locus_tag="ON550_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3954991.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11675..13240)
                     /locus_tag="ON550_001910"
     CDS             complement(11675..13240)
                     /locus_tag="ON550_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3954992.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13233..14684)
                     /locus_tag="ON550_001911"
     CDS             complement(13233..14684)
                     /locus_tag="ON550_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3954993.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14698..>15070)
                     /locus_tag="ON550_001912"
     CDS             complement(14698..>15070)
                     /locus_tag="ON550_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3954994.1"
                     /translation="MKGKDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKP
                     GEDLIIVTIDGQEDMIEKLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKD
                     IFISETVFSQQNIANIPTRNY"
BASE COUNT         4821 a         2572 c         3393 g         4284 t
ORIGIN      
        1 tcttttagtt tattatattt tcttatcaat aataggaacc tgattttaaa agcggctacc
       61 tctttaccgt gcctctttac atacaaaaaa tagaggaatt aagcgtaaat ggcttggcta
      121 ttataactaa aatgctggtt atggagatgt tgcagcaagt tttcgagaaa agcgtattcc
      181 atctcttgtc cattgaataa gatctggata cggtaatgat ttttgaaacg cttctgttca
      241 tgaatctgac tgattgaaag ttcgcctttg tgttctgtct gataaagaaa agtcctttta
      301 cggcattcag cacagcgctt ggttgctcgg cggatttttc ggaatgtata aaaactagta
      361 tctttattgc ataaataaac gtctttgatc gacagcaaat cattatatag acaggaacgt
      421 tcttcagctg ttaattctga taaaaaaagc tgaccttgtt ttattgcatc ttccatcggc
      481 aagtgcctcc tcgaacccat tatccaaatt tttagttaat tctaatatac catcattaag
      541 tataaatgta aacgcttgtt gatggtataa gattttcgtt tctgataaaa cacgaacatt
      601 aaaagaatct aatgccttaa atgtacatat ttttgttgat ttataaaaag atgattgcta
      661 aaatttaaag tgaaaaaacg aacaaaaaac gatgtatttg cttttaatgt aaataaaaag
      721 gagagacgta catggagaag aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc
      781 ttacttctga tgagaacttg atttggatag aatatttaga tcaagcaact gcgttgaatg
      841 gcgcgaggaa agaccagata agtggaaaag gagaattgaa taatcaaatc acaagtgcaa
      901 ttttccgtta tctgactcaa agaggaataa aaaatcattt tattgaacaa ttgtctaaaa
      961 ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga agtggttatc cgaaatattg
     1021 cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa
     1081 tcattgaatt ctactataag gatgaccagt tggatgatcc atttatcaat gaagagcata
     1141 tctatttctt acaacttgca aagccggaag aaatcagtga gctgaaagcc caagcgttgg
     1201 ctattaatga aattttgacc gaattatttg ctaaggtcgg tttggtttta gtagatttca
     1261 agctagaatt tggccggtcg aatggacaaa ttttgcttgc ggatgaagta acaccggata
     1321 cttgccgatt atgggatgaa cgtacgaatg aacatatgga caaagacgtt taccgcagag
     1381 atctaggtga tttgatccca gtctatcaag aagtatataa ccgtctatcg gaactaactg
     1441 aagaaaagga gcgagtatga tgtatttcgt aaaagtatat gtaacctata aagattcagt
     1501 gttagatcct caaggggagg cagtaaaaaa agcagtccat cgtatgggat atgaaacaat
     1561 cgaagatatc cgaataggaa aatactttga gatacaactt gctgatgcag aagaaccaat
     1621 cgatacagtg attgaaacga tttgtgacaa gctgcttgca aatgtaaata tggaaacata
     1681 ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa atttgcggtc atcgtttttc
     1741 caggttcaaa ttgtgacgca gatatgttat gggcagttcg cgagatcatg ggcgctgatg
     1801 ccgaatttgt acggcatgat gagtcctctt tagaaggatt tgatggggtt ttactgccgg
     1861 gaggattctc ctatggtgac tatttgagat gcggtgcgat tgctcggttt tctacaatca
     1921 tggaagaagt gattcgtttt gctgaagaag ggaaaccagt attcggtaca tgcaatggat
     1981 tccagatatt gacagaagca ggacttttgc caggtgtact gagacgaaat gagtctttgc
     2041 attttatttg caaaactgta ccattgaaag tcaaccccaa gacggccttt acttcagagt
     2101 atgaagccga cgaaataatc tatcttccag ttgctcatgg ggaaggaaat tatttctgtg
     2161 acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat tgcctttact tataatgaca
     2221 acatcaacgg cagcgtggaa aacattgcag ggattacgaa caaagcaggg aatgtgttag
     2281 gaatgatgcc tcatccagaa cgtgcagtag aagacttgct aggatcagaa gatgggaaac
     2341 ggttctttgc ttccatttta aaaaacttcg gaaaggtgac cgtatagatg aaaacattga
     2401 aagaaccgac agctgaagag atcaagaaaa aaagaatcta tgccgaatgg ggattgactg
     2461 atgaagaata tcgaatgatc gaagaagata tcttaggaag aatgcctaat tatacggaaa
     2521 ctggactgtt ttctgttatg tggagcgaac attgttccta taaaaattct aaaccggttc
     2581 tacgcaaatt tcctactagt ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca
     2641 tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc agaaagtcac aatcatcctt
     2701 cagccgtgga gccttatgaa ggagctgcga caggagttgg cggaatcatc agagatattt
     2761 tcagcatggg ggctcgtcca attgcgttgc tagattcatt gaggttcggc gaattagata
     2821 atccaagaac gaaatattta ctagaagaag tagttgcagg tatcggcgga tacggcaatt
     2881 gtatcggcat accgactgta ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc
     2941 cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa acatatccag aaagggcaag
     3001 ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc
     3061 acggagcaac ttttgcctca gaagaattta atcaagaaga agaacagcaa cgttctgctg
     3121 tacaagtagg cgatcctttt atggaaaagc tgctgcttga agcgtgtttg gaattaatct
     3181 tgcatcattc ggattctcta ataggtatac aggatatggg agcagcagga cttgtttcat
     3241 ctagtgcgga aatggcttca aaagctggct caggacttat attgacatta gatgaagtcc
     3301 cacaaagaga aactgggatg acaccttatg aaatgatgtt atcggaatca caagaacgga
     3361 tgttgatttg tgttaaatca ggagaggaag aaaaaatcca agaactattt cagaagtatg
     3421 atctagatgc agtcactatc ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc
     3481 aaggagaaga agtagcaaat ctgccagtcg atgcattagc tgaagatgca ccagtttatc
     3541 ataaagaaat gaaagaacca gctcgaatcg tagaatttca gcagttagct ccttatcagc
     3601 cagttatcga agaacctgga caagtattgc ttgatctgct gcagcaaccg acgattgctt
     3661 caaaacgttc aatctacgaa acttatgatt cacaagtaca aaccaataca gttgtccgtc
     3721 caggaagcga tgctgctgtg atgagggtta gagggacgaa taaagcgtta gctatgacca
     3781 cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat cggaggacaa atagcagtgg
     3841 cagaagctgc tagaaatatt attgctagcg gtggtaagcc cttggcgatc accgactgcc
     3901 tgaactatgg ttctcctgat aaaccagaag ttttttggga attatcgact tcagctgacg
     3961 gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat ttcaggaaat gtctctttat
     4021 ataatgaaac ggatggtcaa gcaatctatc caacaccaat gattggtatg gtcggattga
     4081 ttgaggatca taaacatatc actactcagg aattcaaaaa atcaggggat ctaatctata
     4141 tattaggtaa gacgtttgca gattttgatg gcagtgaatt acaaaagatg cagcttggaa
     4201 gaatcgaagg tgtgatccga aactttgatc tatccattga aaagagaaac caagagttag
     4261 tattgacagc catccaaaac ggattgattg aaagtgctca tgactgttca gaaggtggct
     4321 tggcaattgc actggcagaa tcagctttca aacatcagct agggatatct gtacagtttg
     4381 aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac
     4441 cggaaaataa gacacgattc gaagaaatga tgggagatgc tgctgtttta gcaggtaagg
     4501 tgacggatga agcgataatc gaaatttcag caactgacgg tcagatcaaa atagaaactg
     4561 cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa
     4621 tgaagaatgc gggatcttcg gtgtttgggg tcatccagac gccgctagtg tggcatactt
     4681 cgggctccat agtcttcagc atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg
     4741 aggaaagctt aaggggcatc gagatttggg gctgctttct gaggtattca aagatcaaag
     4801 aaaattgcag cagttaacag gagaagctgc aatcggacat gtacgttatg caacagcagg
     4861 caatggaagt gtggacaata ttcagccttt tctattcaaa tttcatgatc agtcgatcgg
     4921 cttagcgcat aatggtaact tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa
     4981 tggggcgatc ttccattcta actccgatac ggagatattg atgcatttga tccgacgaag
     5041 tgaagaaaca gattttttaa ataaattaaa aagtgctttg aatgaagtca aaggtggctt
     5101 tgcctattta ttaatgacag aaacggcaat gattgccgct ttggacccaa atggttttcg
     5161 tccattagca atcggacaaa tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc
     5221 attagaagtt attggggcac gttttataag agatgtccag cctggtgaga tagtgatcat
     5281 caatgacgaa ggaatccaga ttgattgctt tacgaaggac acgcagcttt ctatttgttc
     5341 gatggaatat atctattttg ccagaccaga ttcaaacatc gcagggatca atgtgcatac
     5401 tgcacggaaa aatatgggac ggaatttagc gaaagaatcg ccagtaaaag cagatatggt
     5461 cgttggcgta cctaattctt ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat
     5521 cccttatgaa atcggattag taaaaaatca atacattgcc cgaacattta tccagccaac
     5581 gcctgagcta agagagcaag gtgttcgaat gaaattatct gctgtacgag gagtagtgga
     5641 aggtaaaaag gtgattatgg tcgatgattc aattgttcgg ggtacgacaa gcagacggat
     5701 cgtccatctt ttgaaagaag caggggcaaa agaagtccat gtgaggattg cttctcctcc
     5761 tttgaaatat ccatgttttt acgggattga tattcaaacg cgtaaagaac tcattgcggc
     5821 aaatcacacg attgatgaaa tcaaagaatg tatcggtgca gattctttag gttttttaag
     5881 tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat gctccgtatt caggattgtg
     5941 tatggcttat ttcaacggag attatccgac gccgttatat gactatgaag aaaaatatca
     6001 ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag accaagaagg gagtatatga
     6061 attcaatggc aaatgcatac gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag
     6121 tagaacggat caagaagcat gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag
     6181 gaggattcgg cggttgtttt gatttatcgg tctttcaatt aaaagagcca gtccttgtct
     6241 cggggaccga tggcgtgggg acgaaattag cagtggcaat cgaagcagac aaacacgata
     6301 caatcgggat cgactgcgta gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc
     6361 cgttatattt cttagactac ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg
     6421 tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa
     6481 cagctgaaat gcctggtatg tactcaggta atgattatga tctagccgga tttgctgtag
     6541 ggatagctga aaaatctcaa ttattgacag gagaaacgat caacccagga gatgtgttgc
     6601 ttggaatagc atctagcggg attcattcaa atgggtactc tcttgttcgc aaaattttct
     6661 ttgagcagca gcagttttct ttatcatcaa aactgccaga attgaaaaaa acacttggcg
     6721 aagaattgct tgagccaacg aaaatctatg tttcttcctt gcttccgctg atcaaagaaa
     6781 agaaaataaa aggagctgca catatcactg gtggagggtt tgtagaaaat atcccgcgaa
     6841 tgttaccaga atctatggct gcacaaatca aacttggctc atggccgatt cttccaatat
     6901 tcggctgttt acaaaaatat ggtgaaattc ctgagatgga gatgtatgaa atcttcaata
     6961 tgggcatcgg tatggtcctg gcagtcgaag aatcgaaagt aaaagaaata gtacacgaaa
     7021 ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc
     7081 gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt tttgcttctg gaaacggaag
     7141 caactttcaa gcgctagccg attatctatc gaaaaaagga ttggaatctt cgattgactg
     7201 gttgttttgt gatcagcctg aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc
     7261 ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt
     7321 gcataaattg aaagaaaaaa agatcgattt gatcgtatta gctggatata tgcgaatcat
     7381 tggaccggta ttgctgaaaa attatgataa aagaatcatc aatatccatc cgtctctttt
     7441 acctgccttc cctggtttgc atggcatacg tgatgccttt gaagctggag taaaagaaac
     7501 gggtgtcaca attcattata tcgaccaagg tgtagatact ggaccaatca tccggcagga
     7561 aaaagtgaga attgaacagg aggacacgtt cgattcttta gaagaaaaaa tccatagagt
     7621 agagcatcga atctatccag aagtgattag tgaaattatt gaaaatggag gctatctgaa
     7681 atgacaagag cattgatcag tgtatcagat aaaactggga ttgtagcatt cgcccaaggt
     7741 ttgattgaac aaggagtaga aatcatttca acaggaggaa caaagcatgc gttggataaa
     7801 gctgggatca aaacgctttc gatcgaagaa gtgacagggt ttcctgaaat gatggatggc
     7861 cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt taggaagacg cgatctccct
     7921 agccatatgg aggcaatgga aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat
     7981 ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg aaatagcaga agctattgaa
     8041 aacattgata tcggtggtcc tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc
     8101 acctctgttg ttgatccatc tgactatgaa gaagtacttt cagaattacg tcagtatggg
     8161 catacttcac tttctttcag acaaaaacta gcggcaaaag tctttcgtca tactgcggca
     8221 tatgatgcac tgatcgctca atacttaacg gattgggtgg aagaaaaaga accggaaaaa
     8281 ttgacactta cgtatgaacg aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc
     8341 gctgcctttt atcagtccgc tcttctggaa aaatattcga tcgcagcagc aaaacaactc
     8401 catgggaaag aactttctta taataatatc cgagatgcag atgctgcgct acggatcatg
     8461 aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg
     8521 acagcatcta cgattttaga agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc
     8581 tttggtggga tcatcgtttt gaatcgagaa gtagatctgc caacagcaga acagatgcat
     8641 aagctgtttc tagaaatcat cattgcccct tcttatacac aagaagcttt ggaattactg
     8701 caaaaaaaga aaaacatccg attactgaca gtagactttg cgcaggaaga aaatcaaaat
     8761 cgagaagaaa cggtctcagt cttaggtgga cttcttgtac aagatcaaga tctggcaata
     8821 gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa
     8881 gcattagcgt ttgcttggag agctgtcaaa catgtgaaat ctaatgcgat cgtactggca
     8941 aacgaacgtc agacagtagg aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa
     9001 attgcgattg aacaagcgca agagaaaatg gaaggtgcag ttttagctag cgatgcttat
     9061 tttccaatgg atgattcggt tgaatatgca gccaaacatg gtataaaagc aatcattcag
     9121 ccaggaggaa gcatcaaaga ccaagattct attgatatgg cgaataaata cgatctagca
     9181 atggtcttta ctgatgttcg gcattttaga cattagagag gagtcggata aatgaacatt
     9241 ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta aaaaactttt agccagtccg
     9301 aaggtggata cggtgtattg tgcgccagga aatccgggga tgacaaaaga tggtatcaat
     9361 tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc attttgttaa gaaagaggct
     9421 attgattgga gtatcgttgg tcctgaagtt cctttgttaa atgggatcgt cgatgatttt
     9481 atggcggaag gattgaaaat cttcggtccg acaaaagcag cagctctgat cgaaggttcg
     9541 aaaagttttg ctaaacaatt aatggatact tatcaaatcc cgacagctaa atccagaagt
     9601 tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg
     9661 aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg
     9721 ctggatgcgg cagaacaaat gttgaaccag catcgatttg gggatagtag caaaaaaatc
     9781 gtcgtagaag agtttttggc aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc
     9841 aaggtgtatc cgatggtcat ttcgcaagat cataagcggg catttgataa cgataaaggg
     9901 ccaaatacag gcggaatggg tgcttattca ccagtaccgc aaatttcaaa aaagatgatt
     9961 gaaaaagctg tggaagaagt tttaaaacca gcggctttgg gaatgaaaga actaggacgc
    10021 tctttcacgg gtgttttata tgcgggtttg attgctacga aggaaggacc aaaagtgatc
    10081 gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt
    10141 gactttgcgc aagtaatcga tgatttatta gagaatcgta cagtagagtt aaagtggcag
    10201 caagaaggat acaatgtagg cgttgtggtt gcagctgccg gttaccccga agaatatgag
    10261 acaggaatgg ttctaccaga ttttctggaa gaagagcttt ctgtttacta tgctggagtt
    10321 tcctcaaata acggcaaact agttggagca ggaggacgtc tttatttagt agaagcatac
    10381 ggtgagacga tcaaagaagc acagaaaaaa atttacaccg cgttaatgga taaaacgaca
    10441 gatggaacat tttatcgtac agatattgga gaaaaagctg ccaatagcga actgatcgtg
    10501 aagtaaatca gctgcttcca tttataaaaa atacaacaaa cataatcgga agcgccgatt
    10561 ttagtgaagc cttttcataa agagtgtggt acacttttgt tgtgtatata aataaggagg
    10621 agttcagatg aaaaaaattc aattaggtac ttctgatcag aaagcagctg cagtgatctt
    10681 aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa gtgattgaga cagcttatga
    10741 aaacggcatt gatttttttg accatgcaga tatttatgga aacggtgaat gtgaagaaat
    10801 ctttgcagat gctctagcaa aaacatcaat caaacgagaa gatcttttta ttcaaacaaa
    10861 atgtggaatc gttccaggtg tgatgtttga tttttctaaa aaacatatca ttgaatctgt
    10921 agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc
    10981 agatacattg gtagaaccag aggaagtagc tgaagcgttt gatgaattgg aacggtcagg
    11041 aaaagttcgt tattttggcg tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa
    11101 atatgttcgt caaccattag tagctaatca gctgcaattt ggcttgaaac atactggaat
    11161 gatcgatcaa gggattcatg tcaatatgac agatgaagca agtatcgatc atgacggtag
    11221 tgtacttgat tattcccgat tgcatgatat gacgatccaa gcatggtcac cataccagta
    11281 tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc cctgaattaa accaaacatt
    11341 agaagaacta gcagctaaat atcaaacgac accaactggc ttggcttctg cttggatttt
    11401 acgccatcca gccaatatgc aagtgatcgc cggtacgatg aacgtagggc gaatcaagga
    11461 aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc
    11521 tgctggaaac attttacctt aaagattgtc ataagcaggt caaaagggct ataaattaaa
    11581 aagacagaat aacttgaaag ctgttttcag cttcgagata ttctgtcttt ttttattgca
    11641 aatggaaaga gtatcattgt acggatttcg tttattagtt atttttgttt ttaaggaaaa
    11701 gctgtgtccg atattctttt ggagaaatcc cttgtttttt cttaaataaa taactgaaat
    11761 agttagggtc attataacca atttcaaaag caatatctga aatacgatag tctgattgct
    11821 gcaataattt ttttgctaat tcgattcttt gttctgtcag ataatcgatg aatgtttggt
    11881 taaacgattg agaaaaaatc gtactgaaat gagagggact taatgcaact tgttgagcga
    11941 cccaagttaa ggacatatct ggatcggtaa aattttcttt gataaaatcc aatgcatggt
    12001 tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa
    12061 tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc gctgattgct aagcgttgac
    12121 tggtggatgt gcaatgaacc atttcttgcg ggattttctt ttctttcagg cagacatgct
    12181 ggagtagctt agtcaagaca aataaacgat ataatcgtgt tcgagttggt gtgccttgtg
    12241 gtttcgtcaa gtgttcgatg tacttagata cttcgttttt cgataatgaa gacaaatcaa
    12301 gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt cgcttcttga atcaaatcct
    12361 catagctgat aatttgttct ttaagggaac tggcggtttc aagtatcatc tgcgtgatct
    12421 caaaagatgc aggtaattcg ctgattcttt ctgcgacagg accaatggcg acagaaaaat
    12481 tatttttttg tcccttttct aattcatgga ccaacgtttg ggctacctga taactggtct
    12541 cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt gatgaatcga gaggaaacag
    12601 cagagaagat cacttgttga ttctccctaa ataaaaactt caaataatca ctgagatgag
    12661 cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa taatacggtc atttttttcc
    12721 ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc
    12781 ctttgaacaa accgttcaag aaatgatttt ttttcaattc gaaaacaaga tcagaagaag
    12841 tgtgcgtttc tttttgctgc aaatctaaag cagttactgc tttttctatt gcttgtttca
    12901 attcgctact tttaatcggt ttaagcagat aatcatctgc ctgtacctgg attgctcctt
    12961 tcatataatc gaaatcgtcg aatccactaa taaatatgag acgagtccaa ggaagtagtt
    13021 tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg catttggata tcagtcagaa
    13081 tgatgtcggg ctgtaagtcg ataattgaag acagtgccat ctcaccatct ccagcttcgc
    13141 cgacataagt gattggataa taagaaccaa ggacagttat ttgttcccgc aagttattgc
    13201 gaatgaggta ttcgtcttcg atgataaaaa ctttatacat gtttgtttcc tccaattgtt
    13261 ttgggagata gagggatcat cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc
    13321 gtgataggtt tttgatcatt tccgtaataa agaaggagac gcttgtttac gttgtaaagt
    13381 ccgtagccga tactgaaatc tgaatcgatc cctttggcta actctttacg tatttctgcc
    13441 agtttttctg cagggatgcc aatcccgttg tctataactt tgaattggat catctcatca
    13501 attttgcatg tggtaatacg aataaatcct ttgtgacgga cgtacttcgt tccatggtat
    13561 acagcatttt caatcaatgg ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt
    13621 tctggtatat cgatcttata gtcaagcata tggccgtatc ttatttttaa gatgactaga
    13681 taatcattga tatggctaat ttctttttca agaggaatcc agtcttgtcc tttgcttaaa
    13741 gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca gagcttcttc aaagcagtct
    13801 tgttcgatca aagaaataat ggcatccaaa ctgttataaa taaaatgggg agtaatctga
    13861 gcttgcaata cacgaacttc actttgtgct aaatggtatt gtttcaaagc gttctcccgt
    13921 aaagactggt ttagatcttc cgccatttta ttcatgctag cggtaagtgt ttgtaattcc
    13981 agtgtttccg gcagggttaa acgaaagcca aggttccctg aagaaagttg ttctgacatc
    14041 tcgatcagct gattgatcgg ttcttggacc tttctctcta aaaagcgacg attctttttc
    14101 gcatagaaaa aaatggtact aagaactcca agttccacta ttgttaatag aatgagcgag
    14161 cggacaagtg tcgtattttt tttgcttgct aagtcgatct ccacacgaac aaaatcttgt
    14221 aagatatctg acagcagttg agtgacagaa tctacctgat tgatgatctc ttcattggta
    14281 tccataaaat cttttgccaa gatattattg atcagctcgt tttgataatc ttccagtgta
    14341 tcgatcgtac gtagcgatac atctaacgta ctaatttctt taagtgtatt tgtattattt
    14401 ttgatctctc gaatatcatt cctaagttct tctaggatat tttcctcatt gaactcatct
    14461 acggttactt ggccagaaat caagtcccac atactttcca aaactttttg atcaaccttc
    14521 aaagcaattg catctgcttt ttggatattt gcgatcgtct gttgataaat caaaagctgt
    14581 tgcgaatata acagtatgct cagtagcaaa gggataacag caaatagaag catggctttg
    14641 tttgcttggg caaaaatctg ttttatgctt aaattttttg tcatgatact tctccagtca
    14701 ataatttctg gttgggatgt tagctatatt ctgctgggaa aagacagtct cggaaataaa
    14761 aatatcttta ggtaatgttt ttcctgtttg aatatttcca aaataacgag taacggtatt
    14821 ggctacatac cagccgacat caggtttaca ctcgactaca caattgacga ttcctgagcg
    14881 tagcttctcg atcatatctt cttgtccatc aatagttaca atgatcaaat cttctccagg
    14941 tttgattgat gtttctttta tcgcagctaa agcaccgtga gtcatttcat cactatggct
    15001 gtataataca tcgatttcgg ataaatcatt tgtttctaaa tatcgtttca tctggtcttt
    15061 tcctttcatc
//
LOCUS       DAJSVF010000061        14587 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSVF010000061 DAJSVF010000000
VERSION     DAJSVF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.064"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(166..732)
                     /locus_tag="ON550_001913"
     CDS             complement(166..732)
                     /locus_tag="ON550_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3954995.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(732..1688)
                     /locus_tag="ON550_001914"
     CDS             complement(732..1688)
                     /locus_tag="ON550_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR3954996.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1688..2344)
                     /locus_tag="ON550_001915"
     CDS             complement(1688..2344)
                     /locus_tag="ON550_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3954997.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2714..4732
                     /locus_tag="ON550_001916"
     CDS             2714..4732
                     /locus_tag="ON550_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3954998.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4802..5008
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5159..7573
                     /locus_tag="ON550_001917"
     CDS             5159..7573
                     /locus_tag="ON550_001917"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR3954999.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7921..9474
                     /locus_tag="ON550_001918"
     CDS             7921..9474
                     /locus_tag="ON550_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3955000.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9855..11639)
                     /locus_tag="ON550_001919"
     CDS             complement(9855..11639)
                     /locus_tag="ON550_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955001.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11654..13375)
                     /locus_tag="ON550_001920"
     CDS             complement(11654..13375)
                     /locus_tag="ON550_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955002.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13475..14236
                     /locus_tag="ON550_001921"
     CDS             13475..14236
                     /locus_tag="ON550_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3955003.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4735 a         2699 c         2908 g         4245 t
ORIGIN      
        1 acagccaatc aaaaacagct tctcatgttt ttgatttggt tggacttatt accgcagata
       61 taagcttttg agcaccgtga ataaagaggt tatgaaatca acgctctaac ctgagtatta
      121 gagaaaaaaa cgcaaaacat atcatttatg ttttgcgttt tttatttatc tttttttctc
      181 aatacaatat aaaataggcg gctggttctt ttggttgata aaccgataat tcagcacatt
      241 atatgtttcc tgtggcagtt tggaacaaaa agaatcaacc atatccagtt ctttttctcc
      301 accttcatga ccatagtaga cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa
      361 tatttcttcc atagctgtgc gtgtagtctc tggcatagtg atgattgctt tatcactttt
      421 tgggagatac cctagattga aaatccctgc tttgagattt tgttgttcag ctatcacttc
      481 tcctaacgtt tcgtggcctt gtagaaacaa tgagacacgt tcttctaact gacgctgttc
      541 tagcctttca cgagtagcat cgattgcttg ttgctggata tcaaaactat agacatgacc
      601 agtttttccg atgtgttctg ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc
      661 tacgacgtga tcccctggtt gcaagatttc ttgcagtaat gtatggctga aatgcaaagc
      721 agtttgcaac atcagacgct cacctttttc cgtacatcat attttccttg gaagctatct
      781 ctgcgaagaa gttcttcatc aattgcattc aagacttccc attttttcaa gctccacatt
      841 ggtccaatca aggaatccca cggcgcatcc cctgtcaaac gatggatgat gatctctttt
      901 ggtatcattt ccagctggtc gcaaatgaca ttgacatagt caggacggct cattaattga
      961 aggcgacctt catgatagtc ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg
     1021 agcttgattc cttggatatc tgaatctaaa atggtgcgtc tgacattttc tttcatcatt
     1081 tctaaacttt cgcctggtaa tccgttgatc aaatgcgtac aaacacggat attatgtttg
     1141 cgtaatttag ccacgccatc taaataagtt tgataatcat gtgctcggtt gatcagtttt
     1201 gaagtttctt caaaagttgt ttgcaatcca agttctaccc ataaatacat ccgctcgttc
     1261 agttcagcaa ggtaatcaac tacttcatct ggcagacaat ccggtcgtgt accgactgat
     1321 aatcctacga cacctggtaa attgactact tgctcaaatc gttctttgat tatttcaact
     1381 ggcgcatggg tgtttgtaaa attttggaaa taaacgatgt attgttggac atgaggccat
     1441 ttttgatgca tcatgtcgat ttctttctgg aattggattg gaagagggtc ttcaggtgca
     1501 acgatcatgt ctcccgaacc agatacactg caaaaagtac aaccgccatg ggcaacagtc
     1561 ccatctcgat tcggacagtc aaatcctcca tcgatcggca ccttgaaaat tttcccgcca
     1621 aactcatttc gtaaagcgta attccaagaa tgatatcttt tatttccttc tgaataagga
     1681 aaagtcatta aaagtccttc tttctgtaag agttatctat ttgttttctt ttttctctat
     1741 acactggata agtcaggcac agccaagaaa gactgagtaa gtagccgccg atgatatcag
     1801 taggaaaatg aacgcccaga taaatgcggc ttatcccgac acttactata ataaacatca
     1861 tcagcaattg aagcaatgtg cgtaaggttc ttttttgaac aaaagctgga agcagaaata
     1921 gtaaagaacc gtaaagaatc atactgcctg ctgcatgtcc actagggaaa ctgtaacttg
     1981 tttcaactac taagtgcgcc aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg
     2041 ggttgatgat tccagcaatt acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt
     2101 ttttcttata ccacatccat aaagccgcac aagcaaataa aatgatgacg gtaagaggat
     2161 taccgaattt agtcataaag agttggtagc tgttcagatc aggataagaa gctctgacaa
     2221 tgcctgtaaa ccactgatcg aaaccagaaa gccacgacag atggaacttt acgatccagc
     2281 ccaaaatgaa aaacaggaca agaaagaagc taccagccac ctgaaatgat gttttatatt
     2341 tcatacaatt cctcctttga agcaatgact tattatagca tttttggtcc agcgaaaaaa
     2401 acggtaaaga gataaaacca atgtatgatt cctctttgaa taaccaatat tcttataaaa
     2461 aaaataactg taatcggaaa gacatttttt ggtaatgcaa atgaaatgtt agggaaataa
     2521 aatcagtgtt tttcgaaatc cttttaaaca agaacgtaga cgtaggaaga acttgaaaat
     2581 gaacagaagt tgttcattac ggtaaattac aatatgatta caaaaaacaa caaagctctt
     2641 aaataacttt taataaatat taagagaagt tataattatt aataatagat aaattgaagt
     2701 tggaggatag aagatggaaa acattgcaag aaaagaacgg agacgtttag aacagaccaa
     2761 acgttttcga aaagcaaaac gaggggcagc aatcgtggga actgcgatgg taggctgttc
     2821 tgtcgctgcc ccattagtac agccggttcc tgttcaagcg gatgaaacac ctgttcagtt
     2881 cggcgcaaga atcaatacag cggcatttat cacagaaatt gctacttatg ctcaaccaat
     2941 cgctcaagcc aacgatctat atgcatcagt gatgatcgca caagcagttg tagaaagcgg
     3001 ttggggcagt agtgcgttat ctcaagcacc ttattataat ctttttggga taaaaggcag
     3061 ctatcaagga caaaccgtct acatggatac gcttgaattt ttaaataatc aatgggtaaa
     3121 taaaaaggag cctttccgac aatatccttc ttttgctgaa tctttcaatg acaatgccta
     3181 tgtacttaga aatacttcat ttggcaacgg ttattactat gccggtactt ggaagagcaa
     3241 cacgaaatcc tatacagatg cgacagcttg tttgacaggg cgttatgcga ctgatcctgg
     3301 ttacgcaggc aaattaaata atatcatcgc aacttacgga ttgacccaat atgatacacc
     3361 agctagcgga aacgcaggcg gtggagcgac tacaggtggc aataatactg gaagtgcagg
     3421 tagtactggc ggttcaagca attctggtaa tacaaactca ggttcagcaa cttcgaccac
     3481 ttatactgtc caatctggtg attctgtttg gggaatctca aataaattcg gtatcacaat
     3541 ggatcaattg atccaatgga acaacatcca aaacaatttc atttatccag gacaaaaact
     3601 gacaatcaaa ggtagccagg aaaatggctc ttcgacgaat aattccggta acaataccaa
     3661 ctcttcagga aatgctggta catcaaacgg aggacaaaca actggtgcga aatacactgt
     3721 tcaatctggt gattctgttt ggaagatctc aaatgatcac ggaattacga tggctcaatt
     3781 gattgaatgg aacaatatca agaacaattt cgtttatcca ggacaacagc taatcgtatc
     3841 aaatggcggt tcttctaatg ctggagcaaa tactggctca aatactggga atacaagtgg
     3901 ctctagttcg tccagctcaa gttcttctaa tacagcttca ggaacgaaat atacagttaa
     3961 agccggagac tctgtttgga gcgtctcaaa taaatacgga attacgatgg ctcagctaat
     4021 ccaatggaac aatatccaaa ataactttat ctacccagga caacaactga ctgtttcaaa
     4081 cagtggatca acaaacagca cgtcaggtaa tacaggttcg tcaaccaata caaataacag
     4141 cagttcagtt aatactggca gcagttcgtc tcaaggcact tataccgtga aagcaggtga
     4201 atctgtttgg agcgtggcaa ataaaaacgg catcacgatg gatcagttaa tcgaatggaa
     4261 taatatcaaa aacaacttca tttatccagg acagcaattg atcgttaaag gtggaacttc
     4321 ggcaaataca aatacaggtt caactacttc tgcagcgaaa ccaaatacac cgaatacgtc
     4381 tgctacgaca agtacatcat ccactggtaa tacgatgtat accgtgaaag cgggcgagtc
     4441 agtttggagc gtagcgaaca aacatcatat cacgatggat cagttaatcg aatggaataa
     4501 tatcaaaaat aatttcatct atccaggaca gcaagtgatt gtgaagaaag gttcatctca
     4561 atcaacaaac caacaatcag ttactggttc gaaaacctat actgtgaaat ctggagaatc
     4621 tgtttggagt gttgctgaca gtcatggaat tacaatggct caactaatcg aatggaacaa
     4681 tattaagaac aactttattt atccaggtca aacgttgatt gtagctaaat aaaatagctg
     4741 ttcgaagaaa taacgattga attccacaaa aatatttgtt actataaggc tacttaaata
     4801 tgacaacact tgaaaaggaa gtagtaaaaa gactgaagtt tcagagagcg catggctggt
     4861 gagaatgtgc acggatgctt ttgaactcgc ctttgagttg cccttgctga atacccgagt
     4921 aagcaagcgg cggtgacggc cgttacaacg ttcgaggcct tttcactaag gtaaaattag
     4981 gtggtaccgc gtagattacg tcctataaaa agagcaatct ttttatagga tttttttatt
     5041 tcttttgtga agtttgagaa aacaaagcaa aaggaaataa aaataaacag attctgccgg
     5101 ttttttcgtt atatttataa tagaaacaag tgttttcaat agaacaggag gacagcaaat
     5161 gagttacaat cataaagaaa ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac
     5221 tttcaacacc catgatgatc ctgaaaagcc aaaattttac gcactggata tgtttcccta
     5281 tccatccggt caagggctac atgtagggca tcctgaaggt tatacggcaa cggatatcct
     5341 ttcacgtgtg aaaagaagtc aaggatacaa cgtgctgcat ccaatggggt gggatgcatt
     5401 cggtctgcct gctgaacaat atgcgctaga tacaggaaac gatccagctg agtttaccca
     5461 aaaaaatatc gaaacattcc gacgccaaat caattcttta ggatttagtt atgactggaa
     5521 tcgcgaagtg aatacaactg atccggagta ttataaatgg acacaatgga tctttacgaa
     5581 gctttatgaa aaaggtctag cttatgaagc agaagtagcg gtaaactggg tacctgaatt
     5641 aggaacagtt atttcaaatg aagaagtgat cgatgggaaa agtgaacgtg gtggctatga
     5701 tgtagtcaga aaaccaatgc gccaatggat gctgaaaatc actgcttatg ctgatcgctt
     5761 attggatgat ctagaattag ttgattggcc agaaagcatt aaagaaatgc aaagaaattg
     5821 gatcggtcgt tcagtaggag caaatattga atttaaagtg gctggtacgg ataaatccta
     5881 taccgtattt acgacacgac cagatactct attcggtgca acttactcgg ttcttgcacc
     5941 tgaactagat cttgttagag aaattacgac ccctgaacaa aaagctgctg tagaagctta
     6001 tatcgaagag acagctaaaa aatcagatct aaagagaacg gatcttgcaa aagaaaaaac
     6061 aggagtattt actggtgctt atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg
     6121 gatcgccgat tatgtattat cttcatacgg cacaggagcc atcatggctg ttccagcaca
     6181 tgatgaacgt gactttgaat tcgctcaaac tttcggacta gaaattcttc cagttatcga
     6241 aggtggagat gttgaaaaag ctgcttacac agaagatggt acgcacatca actctgaatt
     6301 tctagacggc atgaacaaac aagaagcaat cgataaaatg aatgaatggc tagaagaaaa
     6361 tggcgtaggg aaaaaagaag tcagctatcg cttacgtgac tggctattct cccgtcaacg
     6421 ttactggggt gaaccaatcc caatcatcca ttgggaggac ggaacagtca cagctgtccc
     6481 agaagaagag ctaccattac ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg
     6541 agaatctcca ttagcaaata tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa
     6601 aaaaggccgt cgcgaaacga atacaatgcc acaatgggca ggaagctcat ggtattacct
     6661 gcgttatatc gaccctcata acaaaaaaga actagcagat tatgaaaagt tgaagcgttg
     6721 gctaccagtt gacatctaca ttggtggagc agaacatgcg gtacttcatt tactatatgc
     6781 acgattctgg cataaattct tatacgatat cggtgttgtt cctacaaaag aaccattcca
     6841 aaaactatac aatcaaggaa tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg
     6901 tggtaatgta gtcaatcctg atgatgtggt cgaaacttac ggtgcagata cactgcgcat
     6961 gtacgaaatg ttcatggggc cattagatgc ttcgattgct tggagcgaaa atggcttaga
     7021 aggaagccgt aaattcttgg atcgtgtatg gcgtttgatc gttgatgaga acaataaaat
     7081 gcgtgaccgt atcactacat tgaatgatgg caagcttgat aaagtgtatc atcaaacagt
     7141 gaaaaaagtg acagaagact atgaaaatct tcatttcaat acagcaatct cccaattgat
     7201 ggtctttatc aatgaagcct ataaagtgga cgctctgcct tatgaatata tcgaaggatt
     7261 tgttcaacta ctcgcaccga ttgctcctca catgggtgaa gaactatggg caatccttgg
     7321 aaatgatggt ggtatttctt acgcaccatg gccaacatac gatgaagcag cacttgttga
     7381 agacgaagta gaagttgttt tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc
     7441 ccgagactta ggaaaagatg agctagaaaa agcagcatta gccaatgaaa tagtgcaaga
     7501 atatattgaa ggcaaaacag tcagaaaagt tattgcggta ccaaacaagt tagtcaatat
     7561 cgttgctaat taattggctc tttgtcaata aggactgatg agttgtgcaa aatcaaatct
     7621 gggccagaat gaacttcatt ctgtctcaga ttttttgtta tttcaaattg attaattgat
     7681 tgatcaaaaa ggttttgatt ttcatgttct caaacacgtt caatgtcttt ttcttctgaa
     7741 tggtcttgta agagatctct gtaatcatca aatttttgtc tattaatctt agtatttttt
     7801 gatgtataaa ctacctgaaa ttgagagtac ataccaaatt cataaatttt ttattagtgg
     7861 gactagtgat tgtatggatt ttgacgcata atgaatgaag tatgagaaaa ggagagacaa
     7921 atggatcaag aactatttat tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa
     7981 gcgaaaaaaa gagcgaaaag aataatgttc aagcttttaa gcttagtatt tttctctggt
     8041 ttgctttttt tcggagctgt atcgttccta tatcgccata tgagtgcagt agtccgttca
     8101 gctgaaggga tgcaaaaatt aaataatttg tatcactttt cgtggggagt tggcggaata
     8161 acaattgttg tgcttattgt ttttcttcta cttatttggc tccgttacca tcaattgtcg
     8221 cgtcatttcg atcaatggct tagtcaaaga gcacaacagt ttttacataa taaaccaact
     8281 tctcaagatg atgactatct ttattttgag aagacttcga cgagtcaagc ttttcggata
     8341 caaaaaagtg attgccagtt attgctgaga tcacttgatg atttacctat ctacttaggc
     8401 gaaagcaaat caaaattcgg aatgaacaca ttccatattt ttttactaga aaacgaaccc
     8461 agatgggaaa aaagccagtc ttcgcctggt ggatacctga caactaagat gagtacatta
     8521 gtcactggtt ttttcctcat tcttttagct ggaacaatct attatgggag atacactcag
     8581 ccaaaaatgt cttctcatat aaatgctgct tctcacatga caatgaactc aactaacgat
     8641 acgcatatac aaaagacaaa tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg
     8701 acaacacaaa agataaatga tctcaaactt gatacagaga cgcactctct ctatatgaca
     8761 acagatagtg gtgctaattg ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc
     8821 gaatacaccc tgacaacggg gctgattcct gaaggttatt ggatggacaa cacatatgat
     8881 atttccgccg atttctcatg gtttatctat tcttctgggg atgatgtacg tctgctgact
     8941 tcaagagata atggtaaaac atggcaaacg tctattatca cccaatcagt tcagccgata
     9001 cgttatcgaa aagcggtatt ttatggcaac gatggtctat ttatctgtac atcagccagt
     9061 gggatgtctg cagaaacttt gtacatttac actactatgg atggcggaat cacttggata
     9121 aaaagtggaa gtacaatgat caatcagcca gtacaaaatg tcagcttcgt tacaacttct
     9181 ttaggttttg tttcgacacg tgaaagtttg ttttatacaa acaacggagg acgctcattc
     9241 aaagaatcag ttctgtcgat tccggaaaac tatcagacag gggggataga tctttttcaa
     9301 tcaccaaatg aagttactca aataagcgcg aatacgttag agacaaaatt ttatttgttg
     9361 aaaaaaggac caatcgatgc agggaaaatg tttgcctgcc tcttccgttc aactgataac
     9421 ggggaaacat ggcaattcgt tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga
     9481 gagattgtga gcctgacgtt cagcaccgag tcacaagaaa caaattcaaa aaacagcttc
     9541 tcatatttgt tgaatagtgt aactcgtgat tcggagttga caagagcaac aagaagcgat
     9601 tttgaagtat agcttctcgt attttacaag atcgtgtctt attgccgata ggttgtctct
     9661 tgaacaccgt ttattcgtaa aaagggattg tgacaagtct tttgtcacta cttaccgaac
     9721 gaaactgaag gagctatcca aaaagtatca ttagtattat tctttgtgtt tctagtggca
     9781 ccactagtac atttaaaaaa gctatcgatc atggactaat catacatgat cgatagcttt
     9841 ttagataatg tttgttacgc tatctgaaat tgactattat aaagactggc ataaagcgtt
     9901 ggtgattgga gtagttcaga atgtgttcct ttttcgacga tatcgccatt cttcattaca
     9961 aggatcatgt ctgcattttc aatcgttgac aaacggtgtg caatcacaaa acttgtccta
    10021 tcttttgtca cagcttccat tgcttgctga atcaatgctt ctgttcgtgt atctacgcta
    10081 gaagttgctt catctaaaat aacgacagga ggattcgcta gaatgactcg agcaattgtc
    10141 aataattgtt gctgtccttg agataacgtg ccgttttcgc tagaaatgat cgtatcatat
    10201 ccgtctggca gtgttcgaat aaagtgatca cattgtgcga tttcagccgc ttcgataatt
    10261 tgttctcttg tggcatccat tttcccgtag gcgatattat cagctaccgt tccttcaaac
    10321 agccaggtat tttgtaaaac catcccaaaa gaacgacgta gttctccacg tgaaagcttg
    10381 gtaatatcaa gaccgtcgaa ttcgatcttt cctttattca gttcataaaa acgcatcagc
    10441 agattgacta aagtcgtttt tcctgcacca gttggaccaa cgatcgctac cgtttgtttt
    10501 ggctttactg aaaaatcgac ttgattcatc aatattttat ctgatgtgta accaaactgt
    10561 acatttttga aagctatttt tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct
    10621 ggaatttcat ctgtttcatc taatatctca aatatcctat cgattgctgc cattgcggat
    10681 tggatcgagt tgatcacata agaagcggta gaaattggtt cagaaatctg attgatgtac
    10741 tgaagataag cttgtaagaa gccgatcgtc acaccacctg atagaacaag catagcaccg
    10801 aataccgcac ttatgatgaa ggcgagttga ttaatgaaac gaatggccgg atagatagca
    10861 cagttcataa attgagcacg tttaaaagcg ttgtattgct ttttatttac ttcgtgtata
    10921 gcagcttctg catgagcttg ctgattgaat gttttgacca ctagattacc agctaagatt
    10981 tcttccattc gattatttaa aacacccagt tcatactgat tctgttcaga aatctgtttg
    11041 tttttgttcg caatcttagt tgtaacaatt gaactgccaa cgatcagcaa taagacgagc
    11101 aaagtaagct tgctttcgat ataaaacagc atcagaccag cagcagctat tgttacgaca
    11161 gaagtaaaaa attgattgat acctgtcaat aacacttgag aaagttgatt gagaccggta
    11221 gttgtccgac taatgatgtc acctacttga tgattgtcaa aaaacgacat cggtaatgtt
    11281 ttgaattttt ttgtgatttc ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc
    11341 attgtccgtt cctgaataaa tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat
    11401 aagacaggga acaacaatgc ttgtttgata tcactgaaag aaagctgtcc aatacctgtt
    11461 tttcttattt ctgataagag atcatcgatc ccgattccca tgatcaaggg catggctacg
    11521 actaataggt tcccgaataa actgcataga aacaatccgt aaaaatattt atgttcaggt
    11581 ttgatcattt tccaaaaacg tatcaaagca cgcgtgttca acgttttttt cttttccatc
    11641 gtatcccttc tttctatatt ccttgtgatt ttgcaaactc ctgataagtt tggcaattag
    11701 acagtagttc ttcatgagta ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt
    11761 tatctgcttg ttggattgta ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca
    11821 tgtgtttatg aagggctgct cttaatgctg catctgtttt atagtctaaa gcggagaaac
    11881 tatcatcgaa tacatagatc tctgctggtt tgatcaacgc tcgagcaata catatccgct
    11941 gtttctggcc ccctgaataa ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag
    12001 gaagggtgtc cactagttct tctaattgag cgatcttgat cgctttagac aactcttctg
    12061 ttgaggcata ttcatttccc atctgcaagt tgcttaaaat tgtcccgctg aataagaaag
    12121 ccttctgagg gacataactg atgtgggagc ggatctgttc ttgagtcatt tgagtaagag
    12181 gaataccacc aagggttact tttcctatcg tagggtcact aagtctcaag agaagtttcg
    12241 ccaaactact ttttccagat ccggtcccgc caacgatcgc tgttgttttt cctttaggta
    12301 ttgtgaaatc aatatcttct agaactggat cagagatttc agtatattta aatgtgacat
    12361 gttcaaaagc agctgcattt gtctgatcaa aggttggttc agctactgtt ttgatcgtct
    12421 cttcctcaat ttcgatctct gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg
    12481 atttagggat aatgaccaat accatagctg cgatcatcaa gtaagcaagt gtcagcatag
    12541 agtattcgac tacagcagtc acgacaccga tttgtaatgc tccttctgct acaagatttc
    12601 cgccaaacca caaaatagaa gcataaacga ttcccatgag caaaaaagca attggagtga
    12661 cccaagcaaa tattcggttc actttgatca tttgattagc atagtcagca aagctcttat
    12721 tcgttcgctt ttcttcatat gattgattat caaaggcacg gatcatatta atcccagtaa
    12781 agaattctct caatgtgatg gtgatccgat ccatttttgg ctggatactc aaagataaag
    12841 gtgcccctcg tttcatcagt cgataaacaa tccatacata aaaaacaaca gagatcaatg
    12901 taacccatgt tagtaataga gaataacgaa acatcatgag aatggtgaaa acgcaaataa
    12961 ccggtgctgg taagatcatt tggcaaaata aaacaatcat ttgctggaca ttatcgacat
    13021 cattcgtcat tcttgtcaaa agagatccag aactaaaacg actgatatcc ttcattgaca
    13081 gctgttggaa tttacggaaa tatttctccc gcgtttggaa tccaaaccta gcggaagcca
    13141 atgctgaaaa gtaactagag gcaactgcag cgattgttcc tagaaacgct acaagcagca
    13201 tttcaactcc aatttttttt ataactgagg cttcgccact tgctatacct gtgtcgatca
    13261 gctttgctac taattttggt acacctaatg taccggcgat ttctaaacag atcatcacaa
    13321 aggtaaggag agcgagtcct ttgtttttct gtaagaatgt ttttaagata gacatattat
    13381 tcctcctttt ttcctgatac ttttcgattc tataccttca agtaacttga aggtcaatag
    13441 aaaaaagaat aaactgaaaa agaggtgaga aaaaatgaaa aaaatgtata cgattggcca
    13501 agtatcggaa ctatttgatt tgccaaaatc aacgatccgc tattgggatg aacaaggatt
    13561 aatccgttca agtagacaag aagagaatga ttatcgattg tttgatatcg atgatatttt
    13621 tatgctttat gatatcgttt tttatagaaa attagatata ccgatcaaac agatgaagaa
    13681 tttatatgga aaaacattga cggagttata tgagacactt gatgaaacag aaagaagaat
    13741 ccataaagaa ctggtggtga tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca
    13801 gttgaaattg atgatcgata caaacgagga agaatttcct gtagaagaga tcccttttga
    13861 ctgtatgatc tcaacggagt ttgaagatat tgtggaaatc aaaaaatttc tacctaacta
    13921 ttcaagcttt gggatgatga gtatgccagc ttccagttca cagacgatgt atggattttt
    13981 tatcgaccct agtgaagtac atttatttac tcaagacgtg atttgggaga aaaaagatac
    14041 ggctgtttat cgtcgattct tattgaaatc cgagatgaac catgcggaac gtaataatat
    14101 tttagaaatc agagaaagaa tgtatgaaaa aggatggaaa acaggagaag tgatcggcca
    14161 gtatctattg acgaatacgg atgaaaacaa tatcagaact gaatattatc atgcatggat
    14221 agaaatgaaa aaataggaaa tatgtgttta agataactcg aaaatgatgg ttgttttagt
    14281 tataaagaaa aattttctgg ttcgattacg ggtttaatgt gggggattat tagtcgtgga
    14341 tagatagaat tttagttttt tagaggaaat atgtctttaa aaatggaatg gttgcaattc
    14401 ttagcgatat atacgatgta attgtcctct taaaaaagta tggaaaaata attgtattgg
    14461 gtgttatgta taattcatgg tgaactgaga ctaatgagaa gaaaaattca tattgttatg
    14521 agtgagattg tatcagatga gaatgataaa ttaaataggt gcataaaagc attacactta
    14581 aaataca
//
LOCUS       DAJSVF010000062        14475 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSVF010000062 DAJSVF010000000
VERSION     DAJSVF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.065"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            91..1431
                     /locus_tag="ON550_001922"
     CDS             91..1431
                     /locus_tag="ON550_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3955004.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1649..2092
                     /locus_tag="ON550_001923"
     CDS             1649..2092
                     /locus_tag="ON550_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3955005.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2596..4014)
                     /gene="nhaC"
                     /locus_tag="ON550_001924"
     CDS             complement(2596..4014)
                     /gene="nhaC"
                     /locus_tag="ON550_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3955006.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4417..5640)
                     /locus_tag="ON550_001925"
     CDS             complement(4417..5640)
                     /locus_tag="ON550_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR3955007.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5671..6510)
                     /locus_tag="ON550_001926"
     CDS             complement(5671..6510)
                     /locus_tag="ON550_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3955008.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6527..7207)
                     /locus_tag="ON550_001927"
     CDS             complement(6527..7207)
                     /locus_tag="ON550_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3955009.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7209..8282)
                     /locus_tag="ON550_001928"
     CDS             complement(7209..8282)
                     /locus_tag="ON550_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3955010.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGFEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8338..8576)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8619..8852)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8929..9600)
                     /locus_tag="ON550_001929"
     CDS             complement(8929..9600)
                     /locus_tag="ON550_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3955011.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9687..12365)
                     /gene="mgtA"
                     /locus_tag="ON550_001930"
     CDS             complement(9687..12365)
                     /gene="mgtA"
                     /locus_tag="ON550_001930"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR3955012.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            13024..13461
                     /gene="ndk"
                     /locus_tag="ON550_001931"
     CDS             13024..13461
                     /gene="ndk"
                     /locus_tag="ON550_001931"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR3955013.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13483..13995)
                     /locus_tag="ON550_001932"
                     /pseudo
     CDS             complement(13483..13995)
                     /locus_tag="ON550_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14056..>14475)
                     /locus_tag="ON550_001933"
     CDS             complement(14056..>14475)
                     /locus_tag="ON550_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3955014.1"
                     /translation="LTEAEIVDRLLYYDDSLKRGYEVYQVFLSAIRRQDVPEFVALLK
                     EDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQ
                     NYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         4134 a         3008 c         2600 g         4733 t
ORIGIN      
        1 tgaaaatttg ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt
       61 gattatgaat ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt
      121 agctccagac atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa
      181 ggaagtggcg aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt
      241 gggctgattt tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac
      301 acgcatcacg gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt
      361 actgattggg tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct
      421 gcttcctttt tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg
      481 gctgttttaa ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat
      541 tggctggctt ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt
      601 gtcggtcagc aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat
      661 ggtgggttta ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat
      721 tcttacggcg gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt
      781 gcgattccta aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct
      841 ttgtttttgc ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct
      901 tttgtgatgg tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg
      961 attgtccttg ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg
     1021 ttcttccgtt tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat
     1081 caagtaccgc aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt
     1141 tcttattttg tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta
     1201 ttattgatct ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca
     1261 ttatgggata aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt
     1321 atcctgttca cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt
     1381 tatttcagtt atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag
     1441 aaaattttat tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa
     1501 agtcttgttg caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt
     1561 tcaaaaaccc tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa
     1621 aggaatgaaa aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat
     1681 cggtgtttca acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca
     1741 tatcttcttc agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt
     1801 catcatgctg ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt
     1861 agccgctagt cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt
     1921 caacgacaca gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag
     1981 taaacgagct gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc
     2041 acaagaagaa gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc
     2101 cgtccaaata aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc
     2161 tacttctgga acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag
     2221 aacagccaaa caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat
     2281 gcaagctttt gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt
     2341 agagagacaa atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg
     2401 gtcagctgat tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat
     2461 taagaaaaat tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag
     2521 aaactggctt ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa
     2581 tcccttttta tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt
     2641 cacaaatttt tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga
     2701 aagcaaactg aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc
     2761 tacgaaactt ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc
     2821 tagcgttcta cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc
     2881 attaccaggc agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact
     2941 tgtcatcaat gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc
     3001 catcacggtt gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat
     3061 aatcaatgac acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat
     3121 ttgttggttt ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa
     3181 ggaagtttgt gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat
     3241 cgttaaaatt gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag
     3301 tgaccagatt gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga
     3361 aagacttgcg gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa
     3421 tgccgggaca gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat
     3481 agctgctgct agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc
     3541 tgaaacaata gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa
     3601 aaatgcaatc ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat
     3661 tgcacacaca acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat
     3721 gaccattaga gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa
     3781 aataaaaata aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag
     3841 ccaatctgtc gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa
     3901 gatcgtgata ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat
     3961 aaccattaat acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat
     4021 ctctcctttt ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg
     4081 caattcatcc gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga
     4141 atgactccgc ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat
     4201 tcaaatgtat ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca
     4261 ccgactttct gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt
     4321 taccttccat aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat
     4381 tatttttata actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt
     4441 ttttcttgct actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt
     4501 caacagatca agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc
     4561 tttgtcactt ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga
     4621 gattgctacc gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga
     4681 accgagactt gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc
     4741 atgctggaag aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg
     4801 gaacaatcca aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc
     4861 cactttttca tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc
     4921 agcagcgtaa tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat
     4981 attgacatct tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa
     5041 tgcttccaaa gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta
     5101 aaatttgatg tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc
     5161 tggatggacg cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc
     5221 tttagctgat ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc
     5281 accagtttca tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa
     5341 caaattatgc ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc
     5401 aattgtggga ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc
     5461 tacttctttt tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga
     5521 ataaaactca tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata
     5581 ttctggtaaa cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat
     5641 aaaaaattcc tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg
     5701 cacgcttgat ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc
     5761 gtctactatt ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt
     5821 cacataagga cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt
     5881 aaatccagca tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc
     5941 acgtgctgtt tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat
     6001 atcagaaaca gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg
     6061 caataacaga agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc
     6121 gtctttgata tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac
     6181 agtatttccg atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa
     6241 ttgatgttgg aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt
     6301 gtaatctgta aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac
     6361 gttgtcccat acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt
     6421 gtcagttgta tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt
     6481 aatgactaca aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt
     6541 ttttaatcag ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg
     6601 tagccacaat cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc
     6661 ctaatccgcc tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag
     6721 tcaacgccga gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga
     6781 taccgattgg actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta
     6841 gcagtgcgtt ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta
     6901 aagctgcttt cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag
     6961 aaagcaagat aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa
     7021 atagcagacg gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga
     7081 tgattccaat catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg
     7141 tcgcttcgac aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat
     7201 cagtcatttt atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt
     7261 aaaatattta tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg
     7321 gtttctccag ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca
     7381 ttgccatata aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt
     7441 tcgttcgtct gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg
     7501 gcaaaagcag aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga
     7561 ataaaatctt tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt
     7621 tcaacgattc ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc
     7681 atctcatgag tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat
     7741 gcaaggatct gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat
     7801 accttaggtt cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac
     7861 agttgtttag gataagcaga tgctttttct tcaaaaccga caagttccag tagttcattc
     7921 actttttgac gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg
     7981 tcaaaaatag ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt
     8041 ctttcttttc gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact
     8101 tctcctgctg ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca
     8161 cctgagtaac cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct
     8221 actgcctgta tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc
     8281 attaaaattc ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt
     8341 gaggacgctt ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc
     8401 ttcaagtacg atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat
     8461 gctcgggatg aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct
     8521 accgaagctc tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt
     8581 caaaaaaaaa gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt
     8641 accacctttt ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc
     8701 gttcgtttta acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa
     8761 ggccattttc agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt
     8821 tccaaactta ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg
     8881 aggaaaactg aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt
     8941 ttttttattt aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa
     9001 tttttccttt tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac
     9061 gcctaatcct gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc
     9121 tgaactagct tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt
     9181 gttctccgac acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg
     9241 atccaagata tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg
     9301 acgttctttt aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct
     9361 tgtcagctgt tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg
     9421 gctacctgct gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc
     9481 gtcaacgatt tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc
     9541 acgatacgtt ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat
     9601 ctatacatta atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa
     9661 cagaagcaaa gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt
     9721 ttcttcacag ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag
     9781 taattgccag gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca
     9841 gaaccgacaa taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg
     9901 ataaatggta ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat
     9961 tcgacaaacc atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat
    10021 gtatggaaac ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta
    10081 gtaatatcaa atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat
    10141 gcattccatt tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa
    10201 atgcaggaaa catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa
    10261 aatggcagga acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct
    10321 gtagccttga tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt
    10381 tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc
    10441 acagctgtat ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata
    10501 ccatctccta aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct
    10561 ttttgctgag gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca
    10621 tgatcactca tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact
    10681 tgacggcaca ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca
    10741 ccatgttctt tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa
    10801 gcaagataac caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg
    10861 cttggattgg tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt
    10921 acttttgcaa gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga
    10981 tcaataaatt tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt
    11041 ttaccagctg gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt
    11101 tcatccactt tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg
    11161 atagccacat ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg
    11221 cgcaaaacac gatcatcttc atttccttca caatccatat gatattcaag aatgatttta
    11281 tcttgtgtca atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg
    11341 atggcattca aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc
    11401 aaattcgtcg taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt
    11461 ccaaaaagaa aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct
    11521 ggagccatca atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca
    11581 aagcttgatt gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca
    11641 tttccaacag aaacaatgat tccttccgca cttccactga tgacattact tcccataaag
    11701 acaagatttt cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt
    11761 tctactggat agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac
    11821 aataaacgca tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag
    11881 acgatttctt ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact
    11941 ttcacaagtg atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc
    12001 attgtcccgc tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct
    12061 ccaggagctg ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact
    12121 aatgtaaatg gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca
    12181 tatgaaatcg cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat
    12241 ccatttgctg atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct
    12301 gcatagattt tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa
    12361 gacagctgtt ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact
    12421 gaaaggggct gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc
    12481 gaatgactgt catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc
    12541 caatcatttt tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga
    12601 tggggatttg cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag
    12661 catattaaaa aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa
    12721 gcagatacct gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc
    12781 cattggcgtc tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa
    12841 caaatcttat tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa
    12901 ttttccatgt ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt
    12961 tttatttgtc tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct
    13021 gttatggaaa gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga
    13081 agcatcatcc aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact
    13141 atgactcctg aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat
    13201 gaactgatcg attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa
    13261 gtgatcgata tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt
    13321 acgatccgag gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc
    13381 cgtgatgctg cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg
    13441 tctgaaaaag cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc
    13501 aatggccagc agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag
    13561 cactgtgaag aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa
    13621 tctttgataa taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc
    13681 taagctccgt ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat
    13741 ataaaacgaa gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg
    13801 tgctaatcgt cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact
    13861 gaatatccaa ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg
    13921 catcaagtat tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga
    13981 tacttctgtt ttttggctct tcgtcaatga gtgttggtga agaaattcaa tagaataatc
    14041 gagatactca cacggctaga cagctatctt gtctagccgt gttgttgttc tggctttagt
    14101 cagttgggta gtgttcttag ttttcttgaa atattttcct cgatataaaa agattcgttc
    14161 tcggtagttt tgaaagtttc taaagccata ggcaattcgt tttagtactt tgatatggtt
    14221 gttcaaacat tctatagggc cgttggaata gggaactcgc aacgctcgtt tgatttctgt
    14281 tcgatatttc ttgaaggtcg tgaacactgg ttgatagtgc tctggcaact ccttgtagtc
    14341 ttcttttagg agcgcgacga attcaggaac atcttgtctt ctgatagcgg aaagaaaaac
    14401 ctggtagact tcgtagcctc gctttaagga gtcatcataa tataatagcc gatcgactat
    14461 ctccgcttct gtcag
//
LOCUS       DAJSVF010000063        14055 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSVF010000063 DAJSVF010000000
VERSION     DAJSVF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.066"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            269..643
                     /locus_tag="ON550_001934"
     CDS             269..643
                     /locus_tag="ON550_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3955015.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(757..1386)
                     /locus_tag="ON550_001935"
     CDS             complement(757..1386)
                     /locus_tag="ON550_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3955016.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1520..1592)
                     /locus_tag="ON550_001936"
     tRNA            complement(1520..1592)
                     /locus_tag="ON550_001936"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1557..1559),aa:Thr,seq:ggt)
     gene            1806..1879
                     /locus_tag="ON550_001937"
     tRNA            1806..1879
                     /locus_tag="ON550_001937"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1840..1842,aa:Asn,seq:gtt)
     gene            complement(2017..2181)
                     /locus_tag="ON550_001938"
     CDS             complement(2017..2181)
                     /locus_tag="ON550_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955017.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2476..3804
                     /locus_tag="ON550_001939"
     CDS             2476..3804
                     /locus_tag="ON550_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR3955018.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            4006..4839
                     /locus_tag="ON550_001940"
     CDS             4006..4839
                     /locus_tag="ON550_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR3955019.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4855..5592
                     /locus_tag="ON550_001941"
     CDS             4855..5592
                     /locus_tag="ON550_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR3955020.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5747..6715)
                     /locus_tag="ON550_001942"
     CDS             complement(5747..6715)
                     /locus_tag="ON550_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR3955021.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6947..7780
                     /locus_tag="ON550_001943"
     CDS             6947..7780
                     /locus_tag="ON550_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR3955022.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7814..8653
                     /locus_tag="ON550_001944"
     CDS             7814..8653
                     /locus_tag="ON550_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3955023.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8679..9239
                     /locus_tag="ON550_001945"
     CDS             8679..9239
                     /locus_tag="ON550_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3955024.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9438..11159
                     /locus_tag="ON550_001946"
     CDS             9438..11159
                     /locus_tag="ON550_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR3955025.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11324..12466
                     /locus_tag="ON550_001947"
     CDS             11324..12466
                     /locus_tag="ON550_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3955026.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12482..13693
                     /gene="thiI"
                     /locus_tag="ON550_001948"
     CDS             12482..13693
                     /gene="thiI"
                     /locus_tag="ON550_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR3955027.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4785 a         2323 c         2854 g         4093 t
ORIGIN      
        1 aagattgacg atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc tcttagacat
       61 aaaaattcct cctggtatgt tttcttctag tctacacacc ttaatttttc tgtctagtta
      121 attgtagcct atccaagtct acactctgag atgtaagaaa tatcataaga tatttcttac
      181 atctttttgt tttgagcagg actttagata attttattaa tatatgctag catgcttttg
      241 taagaaaaag aaagagggcg tattagttat gtatcttaaa attacggaac aagcacaaga
      301 aagattacaa aaatatattg atgaaggcgc aacagttata ttggatttag atgatggcgt
      361 tggggaatat tctaaaatgg gtgtttgctc tttagatact agttttcgtt tacttcttct
      421 tgataaagaa cagagaaaaa atgattataa actccaatta gatagtgata ttggggatgt
      481 ttatataaaa gattattcaa aaatgtacat ggatgaacat atgacccttt ctttagatcc
      541 acgtctaggt gtatttactc ttgaaagccc aagtggttct ttagacagtc atgttcaatt
      601 agtagatcta cgaccagaaa aattctctgg aataaaacaa taactttctc tctattgtcc
      661 ataaaactag tctcaatcaa gctgtattga aaaaacaaga caacattctt accatggtaa
      721 ataaaaagac gacacatttt gtcgtctttt tttaaattaa ctattttcga taaaagatcg
      781 catagcttgt gccattattt ctggcttttc tctattcaat tcatgttttc cgtttttaat
      841 aaagagtagc cggccattcg ttgaaagttt atataaacgt tttgctgctg gtttattcgc
      901 atgatctttg gtaccgcata aaatcaattt aggaagtcgt gatgtacgaa gctgtttttt
      961 gcagtctaat gtttcaagtg aacgaagtag ggtaagggtt tgttttttag atagacctaa
     1021 tttttcaaat atcttagaag gtacgcagta aaaataaata ttctggaaag taagcaataa
     1081 gcgattagga gactcaaatt gtggagcgac taaaacaacc ttttttaaat atggagaagg
     1141 agttgcaaga tagtttaaag caataacagc gcctaaagat aatccacata aaataaaagg
     1201 ttcctttatc tgattcaatt gtacttcgat gtatttggtt aagtcgtcta aagtagcagt
     1261 gtctgtgctg tttaaatgat tgaacaatga aagacttttg caagaataac tattgggtag
     1321 ataagagatt acatctttcc atgattgttc gtcttggcct agaccatgca aaaaaacaat
     1381 tttcatattg ctctcctttt tatagttatc atatagactt tctttgtact attaaaatat
     1441 aaacattcat ttcttacgtc ataaaataaa aaaacagcgc agacacattg ttaaaccaac
     1501 atgttgcact gtttaaggaa gccacttgcc gggcttgaac cggcgacctc ttccttacca
     1561 tggaagcgct ctaccagctg agctaaagcg gcattatcat taatacaaat aataggataa
     1621 cgaatattaa tgtatttgtc aacagaattt tttcgaaaaa atgtgtttct ctaataggaa
     1681 aacagatttt agaaaaaata aaaataacaa aaaatgcgaa cggtttcttt gataatataa
     1741 taaaaaaatt caaaaaaagt attgcgttat ttagaagaaa ttgatattat attcatgtac
     1801 ttgattccgg catagctcag ttggtagtag cgcatgactg ttaatcatga tgtcgtaggt
     1861 tcgagtccta ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg
     1921 ggtttctttt tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg
     1981 taatctttac gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct
     2041 aaatacgaaa taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg
     2101 gatgacattc ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc
     2161 gccagcaatc aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct
     2221 ttcaatgagt attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat
     2281 tatacaggcc attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat
     2341 accgtatgac atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca
     2401 tcatagaaga gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt
     2461 tggaaggatg attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag
     2521 tttcaagcaa atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact
     2581 gcttctgctg aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg
     2641 gctacgggga aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg
     2701 aacacattcc gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa
     2761 aattacactt ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt
     2821 gcaacaggaa aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa
     2881 caagatggcg gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct
     2941 aaatcggtga tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg
     3001 aacaaattac tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca
     3061 ttagacgcta ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct
     3121 atctcattag gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac
     3181 tatcatcttg accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa
     3241 ttagatgatt atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct
     3301 tatacagtag agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt
     3361 gatatggaaa ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg
     3421 tttggttgtg atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct
     3481 tattcaatgg atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat
     3541 tttggagaaa gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg
     3601 caatcaacaa aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc
     3661 tttgttatga gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat
     3721 ttattgacga aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg
     3781 gatccaatgg gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa
     3841 aaagagctgg cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt
     3901 ccctgtgcta taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa
     3961 atatgatgac gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact
     4021 gttttatctg atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat
     4081 cagacctatc gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta
     4141 aaaaaaggat tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt
     4201 gtaccggata cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg
     4261 atcgagccgg gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag
     4321 caaacagcgc cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa
     4381 aattcttttg aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt
     4441 tctttggcag aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt
     4501 aaagaacgtg tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga
     4561 gacctgtgga gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca
     4621 gctgtttctt atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc
     4681 cgaccggaat ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac
     4741 cgactgccga tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt
     4801 tacggttccc aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca
     4861 aaaaaatata ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg
     4921 acatttcctt cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg
     4981 ggaagaggca gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc
     5041 atggagatta atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg
     5101 gagtacaata agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa
     5161 ttagtcaaaa tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt
     5221 gttaatgaag agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag
     5281 gaaaaaattg aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat
     5341 atcgatagta atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta
     5401 ggatatgact ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa
     5461 gtagagtacg ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc
     5521 aaaacaaaac atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg
     5581 aaaaataaat aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat
     5641 ttacgaaaat tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt
     5701 tttgtcacag cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa
     5761 acgaaatcaa ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg
     5821 ctttttgact attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc
     5881 cgccttcata atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta
     5941 cagggatttt ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc
     6001 ccgcacctaa agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata
     6061 agtccccttg catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa
     6121 tgtccagtcg ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta
     6181 agccgatggg acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg
     6241 tcacgtagcg tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg
     6301 cttcttcaca acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa
     6361 gttcattact tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg
     6421 tatcaagaaa aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg
     6481 atatacctga tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca
     6541 tgatgtgtgg tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga
     6601 ttgtgatttc tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca
     6661 tgttttcttt catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac
     6721 agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga
     6781 gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat
     6841 taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct
     6901 tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg
     6961 attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga
     7021 atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa
     7081 gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt
     7141 ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat
     7201 gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt
     7261 tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa
     7321 acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta
     7381 cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc
     7441 catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa
     7501 tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga
     7561 taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact
     7621 agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa
     7681 taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa
     7741 gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa
     7801 aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa
     7861 tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat
     7921 tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt
     7981 ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat
     8041 tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa
     8101 ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt
     8161 aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg
     8221 tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac
     8281 gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca
     8341 gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt
     8401 agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt
     8461 cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct
     8521 gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc
     8581 agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg
     8641 tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc
     8701 aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata cccacggctt
     8761 ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg
     8821 ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag
     8881 cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg
     8941 ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct ttttcattac
     9001 taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat
     9061 ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact
     9121 ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag
     9181 aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat
     9241 cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag
     9301 ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga
     9361 cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga
     9421 aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta
     9481 tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat
     9541 tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg
     9601 atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc
     9661 aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag
     9721 tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag
     9781 aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat
     9841 tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag
     9901 aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc
     9961 aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg
    10021 gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg
    10081 aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac
    10141 aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag
    10201 aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca
    10261 atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga
    10321 ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta
    10381 accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga
    10441 ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac
    10501 gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga
    10561 tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag
    10621 tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa
    10681 aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga
    10741 aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt
    10801 ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca
    10861 tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga
    10921 agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc
    10981 gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca
    11041 ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag
    11101 aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa
    11161 gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt
    11221 ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac
    11281 aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa
    11341 cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg
    11401 gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca
    11461 gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac
    11521 ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga
    11581 atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga
    11641 acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt
    11701 acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc
    11761 tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga
    11821 ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc
    11881 gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc
    11941 acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg
    12001 tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat
    12061 ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg
    12121 cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga
    12181 aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt
    12241 tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc
    12301 acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac
    12361 atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat
    12421 gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct
    12481 tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg
    12541 aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga
    12601 aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca
    12661 gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg
    12721 tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta
    12781 tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac
    12841 aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca
    12901 agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta
    12961 tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg
    13021 aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg
    13081 gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact
    13141 gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat
    13201 cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg
    13261 gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca
    13321 tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag
    13381 tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga
    13441 taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc
    13501 ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga
    13561 aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc
    13621 tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc
    13681 agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac
    13741 gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga
    13801 ataccattta tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa
    13861 ttttatttag agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa
    13921 atagcgaggt tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt
    13981 gcttttcaaa tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc
    14041 gtgtatcaga ggttg
//
LOCUS       DAJSVF010000064        13954 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSVF010000064 DAJSVF010000000
VERSION     DAJSVF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.067"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            242..580
                     /locus_tag="ON550_001949"
     CDS             242..580
                     /locus_tag="ON550_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955028.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(785..1024)
                     /locus_tag="ON550_001950"
     CDS             complement(785..1024)
                     /locus_tag="ON550_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3955029.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1000..1347)
                     /locus_tag="ON550_001951"
     CDS             complement(1000..1347)
                     /locus_tag="ON550_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3955030.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2079..2660)
                     /locus_tag="ON550_001952"
     CDS             complement(2079..2660)
                     /locus_tag="ON550_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3955031.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2843..3469)
                     /locus_tag="ON550_001953"
     CDS             complement(2843..3469)
                     /locus_tag="ON550_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3955032.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3491..4822)
                     /locus_tag="ON550_001954"
     CDS             complement(3491..4822)
                     /locus_tag="ON550_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR3955033.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4977..5378)
                     /locus_tag="ON550_001955"
     CDS             complement(4977..5378)
                     /locus_tag="ON550_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3955034.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5464..5670)
                     /locus_tag="ON550_001956"
     CDS             complement(5464..5670)
                     /locus_tag="ON550_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955035.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5765..7267)
                     /locus_tag="ON550_001957"
     CDS             complement(5765..7267)
                     /locus_tag="ON550_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR3955036.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7285..7740)
                     /gene="argR"
                     /locus_tag="ON550_001958"
     CDS             complement(7285..7740)
                     /gene="argR"
                     /locus_tag="ON550_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3955037.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7838..8455)
                     /locus_tag="ON550_001959"
     CDS             complement(7838..8455)
                     /locus_tag="ON550_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR3955038.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8676..10049
                     /locus_tag="ON550_001960"
     CDS             8676..10049
                     /locus_tag="ON550_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3955039.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10124..11236
                     /locus_tag="ON550_001961"
     CDS             10124..11236
                     /locus_tag="ON550_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3955040.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11308..12945)
                     /locus_tag="ON550_001962"
     CDS             complement(11308..12945)
                     /locus_tag="ON550_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR3955041.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13113..13721)
                     /locus_tag="ON550_001963"
     CDS             complement(13113..13721)
                     /locus_tag="ON550_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3955042.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3791 a         2914 c         2480 g         4769 t
ORIGIN      
        1 cgcaaaattc ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg
       61 gatataaata gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca
      121 agaaacattg aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac
      181 cttcaatttt gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat
      241 catgcccgat tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg
      301 aaagttcacg aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct
      361 tcaaatagaa acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg
      421 tgagatcgta gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt
      481 ggataaatgg caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt
      541 aactttgaat tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat
      601 aaataaaaaa gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc
      661 gactgtgacc ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt
      721 ttttgggatc tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat
      781 tatgttatac cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa
      841 aagaaatatc tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt
      901 catggattgg tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt
      961 tttcatcctg atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa
     1021 acataaattt gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa
     1081 tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg
     1141 tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc
     1201 ctcttttctt aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc
     1261 tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc
     1321 tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac
     1381 actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt
     1441 gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt
     1501 cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg
     1561 aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa
     1621 gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa
     1681 ctaagcgact ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
     1741 ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt
     1801 gatgcagggc agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg
     1861 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
     1921 tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc
     1981 gaaaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
     2041 ttcttgaagc tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt
     2101 aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact
     2161 ttcaacaata agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct
     2221 tcttcatcgg acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc
     2281 cattgattga ctaattctag tagcaacaga cggtattctt catccactgt actcaactca
     2341 ataatcaaat tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa
     2401 atccagtcca acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct
     2461 tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct
     2521 ttttttgaag aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata
     2581 tctcggattg acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag
     2641 atcttttcct ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata
     2701 cagtaaattc atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga
     2761 cacagccctc actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc
     2821 gaggacaaaa gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca
     2881 cttgatctgc aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag
     2941 caccaattgc atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat
     3001 gggaatcgct acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt
     3061 ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga
     3121 tgatcgtatt ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt
     3181 cttccactga ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt
     3241 ctcctgcacc gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa
     3301 agagtttgta tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat
     3361 tgtatttttc taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact
     3421 cgacttgcgg gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc
     3481 cattcgttat ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga
     3541 tttgatctct caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga
     3601 tgacttcatg agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt
     3661 cgactaaaac tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc
     3721 ttgtcatata cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta
     3781 aacctacatt ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat
     3841 tcgttgttcg tttattctcg cccttcaact gatccataat gattcgataa tcatctagag
     3901 agatataagt gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat
     3961 ctgtacgaag gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat
     4021 tttctaatcc aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg
     4081 cttggattgt tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt
     4141 ctgctacttg atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt
     4201 ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa
     4261 gaacgacaac tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc
     4321 cagccccgat gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat
     4381 caatcaagta gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag
     4441 tattgatgaa aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga
     4501 tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc
     4561 tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag
     4621 cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc
     4681 cgatgttgat acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt
     4741 caccttttga agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa
     4801 tattttgata tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa
     4861 aaatatgata accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa
     4921 ccagacaaaa agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa
     4981 acaatcgtta cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca
     5041 tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct
     5101 aatactacac cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt
     5161 actgtggact ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa
     5221 caagaagcat aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct
     5281 ggaggagtga ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg
     5341 ccatggttga tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac
     5401 catttgctac ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc
     5461 tgcttattcg aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact
     5521 actcgtgaac aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc
     5581 ttttatatga ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac
     5641 tgttaaaaaa ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca
     5701 gcactcgatt taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac
     5761 tggtttattc tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt
     5821 ttactaacgc caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg
     5881 gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat
     5941 atcgaatacc ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc
     6001 gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta
     6061 atcggatttc ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga
     6121 taccttttct ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat
     6181 catccagaaa atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg
     6241 tatctttccc aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa
     6301 tataacgaac ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag
     6361 gggaattcgt cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg
     6421 cgatcaaatt atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca
     6481 catctgtatc gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac
     6541 tttccatcgc ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt
     6601 ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga
     6661 atccaccagc accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt
     6721 ctagtgttcc aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa
     6781 ggtctgcttt ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga
     6841 taaaagcaat atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat
     6901 aataataggg aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa
     6961 attgagtgga cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc
     7021 cttgttcttt cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt
     7081 catgaccaat cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta
     7141 tccagtttgc tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat
     7201 atccttcaac tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat
     7261 ctttcatata aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg
     7321 ctgtgcttct tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc
     7381 gacaacttca gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg
     7441 ttcagtcaat acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat
     7501 tgctgtacgc aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg
     7561 tactattttg ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat
     7621 cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt
     7681 cccaataggt tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat
     7741 attagacacc tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg
     7801 tattttttac ttttattttt tgttttcatt caggttgcta acctttttct attttaacac
     7861 gcaaaatcgt cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt
     7921 tgtgttcttt agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa
     7981 acgttgctgg ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt
     8041 ccatcttttt gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa
     8101 tggcttcttg tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag
     8161 aaaaatgttc ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc
     8221 cttccagtgg atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg
     8281 acatgcgtat tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct
     8341 gattcgggtc gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt
     8401 gcgggctttt tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc
     8461 ttcctccgcg aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct
     8521 aatcgtgttg acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca
     8581 gataacacgt ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact
     8641 ctcttttttt acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt
     8701 tagtacctgc agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg
     8761 ccatgaatgt aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc
     8821 aatgggtaac aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt
     8881 tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag
     8941 gtataatgat cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc
     9001 aaggtgcagc gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc
     9061 ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag
     9121 caattggccc cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc
     9181 tgtttcttgt gcccgttttg tttctttctt tgattatcgg ctggtttgct attcccgaga
     9241 tggcagtaca aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt
     9301 ttagtggaag tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa
     9361 tcatcggcgg tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac
     9421 tgatccgact gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag
     9481 tttatcagtt tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc
     9541 aaggaagtag tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt
     9601 tgctttctcc tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg
     9661 ctgggctgat tctttctttg attggttggc tagcattatt cctactcata ggacacgctt
     9721 cagtgggtgt attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca
     9781 gcaatatgat gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg
     9841 caatgttcaa taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca
     9901 ttatcaatta cgtccagcat catacttccc ataactatga agtgtctact acattaggat
     9961 caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct
    10021 attatctgtt ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg
    10081 tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat
    10141 tatcggtggc gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa
    10201 tgttgcttta tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc
    10261 cccttggttg tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc
    10321 ttttttccct aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg
    10381 gacacttttg cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg
    10441 aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca
    10501 agcacttccg ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga
    10561 tggaaaagca tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat
    10621 ccaggcaaaa gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg
    10681 agaatacaca gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca
    10741 agaattaggc tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac
    10801 atcttataag atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat
    10861 tcctttcatg gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga
    10921 tttggctcct actagcgaag cttataaggg attgactaag agtacagaac agttcttgac
    10981 tgatccagat cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac
    11041 gtcagatttt tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag
    11101 cggtctaggc tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt
    11161 cttaaatact ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac
    11221 gaaaaatcca tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat
    11281 ggattacttc ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact
    11341 ttcttttgct tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg
    11401 aatatcaagt cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa
    11461 atctggtata ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt
    11521 ttgttgcacg attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc
    11581 gaaatatcca gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa
    11641 agcttgatag tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac
    11701 tagctcatct agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa
    11761 tgttgcacca aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga
    11821 atcatctttc atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc
    11881 gggtgtcgca taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac
    11941 taggactttt tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga
    12001 agaaatggcc ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa
    12061 ggcagtagca aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac
    12121 agagtgacct tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc
    12181 aaatacagcc atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca
    12241 ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa
    12301 ttctccaaag taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat
    12361 gagtccattt ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc
    12421 ctcatcaaag gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa
    12481 attcagcaaa taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg
    12541 cacagaaaat tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg
    12601 actttgatac acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc
    12661 ctcaaagatc cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag
    12721 tgtcctaaca tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat
    12781 gatcgttcct ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg
    12841 aacattatta ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt
    12901 tccacttgtg atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc
    12961 cctaaacaaa aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc
    13021 gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag
    13081 tttctcggtt tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat
    13141 gttttagcag catcccatga accataacga gatgttacat attgttctgc tacacgttct
    13201 tgattggcag ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg
    13261 taacgtccgt tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt
    13321 gcagagcttg aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt
    13381 tcaacaggtg caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt
    13441 tctgttgttt tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt
    13501 tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc
    13561 attgagttgt ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact
    13621 gtataaactt catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca
    13681 gcaaaagttg tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct
    13741 ccgtttttta ttctttcgac gctattaact ttatcattta gaaatatttc ataggtaaaa
    13801 gaaacatgac atttaatgac aaaataacga attatttaat atttgtaaca tttaaacaaa
    13861 tatttttttg atacaaaacc ataaaagcct tgttgtataa gcaaagtaat cgtttttcta
    13921 tgttttttta tttttgaatg aaaaacgcag attc
//
LOCUS       DAJSVF010000065        13808 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSVF010000065 DAJSVF010000000
VERSION     DAJSVF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.068"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ON550_001964"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ON550_001964"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3955043.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ON550_001965"
     CDS             complement(2261..2512)
                     /locus_tag="ON550_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR3955044.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ON550_001966"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ON550_001966"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR3955045.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ON550_001967"
     CDS             3462..3680
                     /locus_tag="ON550_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955046.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ON550_001968"
     CDS             complement(3969..4481)
                     /locus_tag="ON550_001968"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR3955047.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ON550_001969"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ON550_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR3955048.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ON550_001970"
     CDS             complement(6934..7377)
                     /locus_tag="ON550_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR3955049.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ON550_001971"
     CDS             complement(7378..8166)
                     /locus_tag="ON550_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3955050.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="ON550_001972"
     CDS             complement(8163..9104)
                     /locus_tag="ON550_001972"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR3955051.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ON550_001973"
     CDS             complement(9281..9868)
                     /locus_tag="ON550_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR3955052.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="ON550_001974"
     CDS             complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="ON550_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR3955053.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ON550_001975"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ON550_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3955054.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ON550_001976"
     CDS             complement(12785..13720)
                     /locus_tag="ON550_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3955055.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3806 a         3096 c         2435 g         4471 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat atcatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgtaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAJSVF010000066        13559 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSVF010000066 DAJSVF010000000
VERSION     DAJSVF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.069"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..761)
                     /locus_tag="ON550_001977"
     CDS             complement(<1..761)
                     /locus_tag="ON550_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955056.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKV"
     gene            complement(772..1440)
                     /locus_tag="ON550_001978"
     CDS             complement(772..1440)
                     /locus_tag="ON550_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3955057.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1571..2155)
                     /locus_tag="ON550_001979"
     CDS             complement(1571..2155)
                     /locus_tag="ON550_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3955058.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2486..2719)
                     /locus_tag="ON550_001980"
     CDS             complement(2486..2719)
                     /locus_tag="ON550_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR3955059.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2734..3681)
                     /locus_tag="ON550_001981"
     CDS             complement(2734..3681)
                     /locus_tag="ON550_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3955060.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3681..4523)
                     /locus_tag="ON550_001982"
     CDS             complement(3681..4523)
                     /locus_tag="ON550_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3955061.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4656..5381)
                     /locus_tag="ON550_001983"
     CDS             complement(4656..5381)
                     /locus_tag="ON550_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3955062.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5842..6156)
                     /locus_tag="ON550_001984"
     CDS             complement(5842..6156)
                     /locus_tag="ON550_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3955063.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(6172..7767)
                     /locus_tag="ON550_001985"
     CDS             complement(6172..7767)
                     /locus_tag="ON550_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR3955064.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7790..8680)
                     /gene="iolE"
                     /locus_tag="ON550_001986"
     CDS             complement(7790..8680)
                     /gene="iolE"
                     /locus_tag="ON550_001986"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HCR3955065.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8697..9722)
                     /gene="iolG"
                     /locus_tag="ON550_001987"
     CDS             complement(8697..9722)
                     /gene="iolG"
                     /locus_tag="ON550_001987"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HCR3955066.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9746..10753)
                     /locus_tag="ON550_001988"
     CDS             complement(9746..10753)
                     /locus_tag="ON550_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3955067.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10866..12026)
                     /locus_tag="ON550_001989"
     CDS             complement(10866..12026)
                     /locus_tag="ON550_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR3955068.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(12102..13556)
                     /locus_tag="ON550_001990"
     CDS             complement(12102..13556)
                     /locus_tag="ON550_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR3955069.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         4065 a         2919 c         2214 g         4361 t
ORIGIN      
        1 cttacctttt cgatataggg aacttcaatc gaactattaa aaatgtcaaa tttccttaat
       61 gcgttattgt tttcagtttt taacaactga atagttgtag tttttacagt tctattttta
      121 acaaatagaa cttgatcaat taattctttc aaatagtaaa aacgatgttc agctataaca
      181 atagtaaatc ctttattttt taaatcacaa agtaagtcag caagtttctt agtcgctttg
      241 aagtccaaat ttgaagacgg ttcatccaat ataattaatt tagagtttag cgataaacta
      301 catccaatag aaattttctg ccgttcacca cttgatagcg aaaaaatgtt tttatctaat
      361 atatttgaaa gatctaataa ttcagctaat tcattgattc ttttatccat atgctctttt
      421 gtaaatccaa agttttccat cgaaaatact aactcactcg ttgtatttgt tgtaaagaat
      481 tgtcctctag gatcttgact tacgtagcca atattttttg atatactttc ttgtgttaag
      541 gattgtagcg agttattttc aaataaaatt tccccaacca attttccgtc ataaagtctt
      601 ggaataagtc cattcattaa ctttaatagt gtggatttcc cactcccaga agaaccagta
      661 atcaatgtaa gactaccttt ttttattgaa aaattaaggt caacaagcgc ttgttcctta
      721 acatccttat atgaaaaaga aacattgtta aattcaaaca attctttcac ctcaaatcaa
      781 aatacttaag aaaaaaatag taacggaaaa caggatgata tagtctaata agctcatgtt
      841 tgttttatag taagaacttc ttttcacagg agattctatc ccctttgtta ttgcattagc
      901 ggtcatttct tcagcaatca tagttgcacg tataagaacg ggaactaaca gaaattccaa
      961 atattgcaga ggctgaaaaa aagacaagcg aatgtttctc atttttaaag attctttaat
     1021 cattcttatt tcagaacgat aagtcggaaa gaatctgatc actactgtaa tacttaacat
     1081 tatttttttc ggtaaatgta ttttttctaa agaggataat aattcattgc tagtatagac
     1141 actaaccaac aaccatgaga tcattacgac cggcaacatt cgaaatacaa tatataagga
     1201 tgtcccaaaa aatatagtaa aaaactctat cttgatagca tcaataattt tcattactaa
     1261 aaacagaact gaataaatca agatgaaata aatacatgtc tttattttac gaaacatcaa
     1321 tactaaaact gtagcaataa tgaaacaccc cacttgaaat atatttccct ttcctaaaaa
     1381 aataatgacc ggaaaaaaaa tatttaaagt aatcaaggtt aacggattga tttttgacac
     1441 tctttcacct ccaaaaaata agatcgcact gttatcttaa gctgataaaa atgcaccgtt
     1501 attcagataa cagtgcgatc ttatcaaaaa gcataactga gaacaattct caatgcaagc
     1561 ttttttttaa ttaactcagt aattcaatcc acccaggaac agaaaattga tacaatcttc
     1621 tggttaatag catagtcttt tcttttgaat agctatgctt caatatatca tagaatgtag
     1681 cgtagaatga gtaaatgtag aagtggattt cttcttcatt aaagactaaa ttttgattga
     1741 tcattccgat agtctttaac ttatctagac aacagactgt cttttttgtc acaaatgatg
     1801 taattttttc aacaatatct tgatatttgg ttccagctga ttggaataaa accaaatcga
     1861 atatacgttt attctcaaaa atataattaa taaatggatc taattcattg aagttctctc
     1921 tccaactttt ctctatatct atattttcta agtttaagag gtatttggta aactcttcat
     1981 tatacatatt aataagcgca ttaatatgcg gctgaataat ttcttcaaat agcgcttcct
     2041 tagaagtaaa atgtttataa aaagcacccg tagttacgcc tgcggtcaag cacagttgcc
     2101 ttatcttagt ttgctgaaat cctacttttt caaaatggct tagtccactt tctattaatt
     2161 tttggtgagt tgtaatataa tctctggttt ctatgggtac ttcctcctaa taagatgaat
     2221 taactatatt atcttacaca ggacttgaag tttcaatcgt taacctctat tactgaactc
     2281 attccttaga gctaaactaa tgagaaagag caaaataact gatacaaaaa gcaatcctac
     2341 aagaattaat agactcatct aaatcattca ccttttctaa aataatccga gaaagattta
     2401 aaaaaattat atccagctat taggataagt cctatcataa tcactccaat agccattttc
     2461 ttatcctccc taaaaataaa gccttttact taaaaccttt tttgtaatcg tcaataaatt
     2521 ttttcccttt atcagttaat tgaaatccta tatctaaata gtcctcatat ctaagtttag
     2581 tcagcatctc acttacagat gctggactaa cacttagaac ttttgctaaa tcattatttt
     2641 ttaatttctt ttttcttttt gataattcct gaattgctag taaataaatt agtatagtag
     2701 ttgttttaat ttgtaccata cctaccctcc aaattatttc gaaaggcctt cagcaatttt
     2761 atccaggttc catttcatca tcgcatagta actatcacca tcttgtcctt tttctgcaat
     2821 tgaatcagta aaaatcgttg aatagatagg aatattggta tcttttgata ctgttttcat
     2881 tggtctatcg tccacactgc tttctacgaa taaggaggga acttttgttg tgcgtaattt
     2941 ttcaactaag tgttttattt gatctggtgt tccttcttct tcagtgttga tttcccaaat
     3001 gtaagcagaa ggcacattat acgctttcga gaaatattta aagcatcctt cacttgtaac
     3061 aatcattttt ttatcattcg gaattgaagc aaatttagat ttagcttctt tgtctagaga
     3121 atccaatttt tcaatatact tatctagatt ttctttatag aattttttat tatcaggatc
     3181 tttttccgct aactgttttt caatattttt agcgtaaata ataccgtttt ctaaatttaa
     3241 ccaagcatgg ggatcttcct tccctttctc actctgaccc tctaagtaaa taacatctat
     3301 gccatcactt gctgcaaaat agtctttgtt ttcctctttg ttcgcatttt ttactaattt
     3361 tgtaaaccaa gcatttcctc cagtttccaa gttaacaccg ttataaaaaa tcaaatctgc
     3421 ttttgaagtt ttttgaacat cttcaggcaa aggttcatat tcgtggggat ctttcccaat
     3481 aggtacgata ctgtgtaaat caattttatc ttttgctata ttttcagtaa tatctgctaa
     3541 aatcgaatta gtaactacta cctttaattt ttcgttcgaa gccactgtat ctttgtcttt
     3601 actatttgtt gaacatgccc cgaatataaa aagagcaatc aaaatcccca gacttcccag
     3661 tactgctttc cattttctca tcataacttt ctctccttct ttcttaaaaa tttttgtttt
     3721 ggtgagatta gaaaactaat tacaaataag aatgccgctg ttaaaacaat acttgatcct
     3781 gctgctatgt taaaactata accaatgaac aaacctagca atgatgagca cgctccgact
     3841 gtcgccgaaa taaatatcat agtttttaaa ctgtttgcat ataaatatgc tgtagcagct
     3901 ggtgtaatta acattgcaac aattaatata gttcccacac tttgcatagc tgttactgct
     3961 actaaagtta atagaatcat taatagataa tgataaaaac tgacattcat ccccattgct
     4021 tttgccatta atggatcaaa agaagtgatt aataattctt taaaaaatag aacaactgta
     4081 accaacacta aaacagaaac gccaattgta atccacttat caatatcttg tacagctaaa
     4141 atatttccaa ataagatatg aaataaatcc gttgagctat ttgcaactcc tataaggata
     4201 actcctaatg ctaaaaaaga actgaaagta ataccaattg ctgtatctcc ttttattaca
     4261 ctattgcttt ttataaaagt aataatgata gaagctaata gaccaaatac aatcgcacca
     4321 ataaaaaaat taatacctaa aatataagaa attgctactc ctggcaaaac agcatgagaa
     4381 atagcatcac ccataagaga cattcctctt aaaataataa aacaacctac tgttccagaa
     4441 acaatcccta taactacaga tgtaattaga gcattctgta aaaagtgaaa gtcatacaga
     4501 ccattaataa aattttgaag catattattc cccttgtata ttagagttag ggttttaacc
     4561 ttccggtttt gattggataa ccctatgaac catcgttatt ttaatttggt attaactcat
     4621 ttcttttgag ataatatatt tattggattg agaggctaag ccgttccccc ctctccaata
     4681 aatatagtat ctccataggc tttttttaaa ttctcttcat taaatacaga ttctacactt
     4741 ccgtgaacaa ttagatttcg attcaaaaga ataatattat caaaatactt ctttaccttg
     4801 cttaaatcat gatgcacaat tagcactgtt tttccctgat gtttcaattc ttttaacgta
     4861 tccataatta ttcgttcact aactaagtct atccctacaa atggttcatc taaaaatatg
     4921 aaatccgcat tttgagcaag acatcttgct aaaagtacac gttgaaattg tcctccagat
     4981 aattctccta tttgatgatt agaatatttt cccatatcga ccttttccaa agcctttgaa
     5041 actctttgcc attctgcatt tttaattctt tgaaaaactt tcattccggc atatgttccc
     5101 atagatacac attcttttac ttttattgga aaagtaaaat caatatcagc tttttgctct
     5161 acatatgcaa tcttcaccag ttttttattg atatcttcat tattaatgga gacctctccc
     5221 tgatgaggga taatatttaa cattgcttta agcaatgtag attttccagc accatttgga
     5281 ccaataatac ctgttaaagc accttcttgt atttttaaag aaatctcgtt caatgctaaa
     5341 aaatcgttat aacaaacatt aagctttttc acttctaaca taactttctc cttctaaatg
     5401 ataatgatta tcacgatcat atggtataca aaaattatat tttagtcaac cctaaatttt
     5461 ataatttaga tttttttctg ttgttgaaga ttaggatgtc aaaattattt gaaactactc
     5521 gtatagtaaa tagtaagtaa ttttcaaaac acgaagaaaa acaaaaagaa aaaaggaaac
     5581 gccgaagcat ttcctttcag tgatttaatg agaacaacac gatttgactt agagtctcaa
     5641 aaagtcgaat aggtacactc atttaagtgt ctccattcga ctttttgggg atgagctggc
     5701 tattgaggtc cgcttaccct attgaataaa tacaagattg ggtatttcta ataagtccgc
     5761 ccttcatatt ggggtgatcg gggcgaacat gccatctttt catataccac ctcctcgaca
     5821 ctcaataatc aaagattctc gttaatcttc ttcgatgctt gcaatgatgt tttcaatatt
     5881 catataacga aaatcttctt gtttgatata aacgatctga gcggtacatt tttccttttt
     5941 gatgatttct tttacttttt cctcagaacg tgcgacctgc ggcgttaaaa gtaaaacgtc
     6001 atagtcttta taattcagtt tttcttcaac gtttcggatc gttgcataat ctatctcata
     6061 cgatagattt ttggctctga tcgtttcaag catttttgat acgagcagcg acgcactgat
     6121 tccggtctcg caaataatca atagatcctt atgcataata ccctcctgtt tttagcctgc
     6181 aaagccaatt ggtgaaaatg ccacataagt taaaataaca atgactacca atgctatagc
     6241 agtcaccttg gtgtatttcc aaggctcgat atcgaccttt gtatcgaatg agttcaacac
     6301 aaattctcca tcaggtttca atttggaaac caccatcaaa atgataatgt ccagaacgaa
     6361 taatgccgtc aaaacataaa ggaaatggat attttggaaa agcatttttg atagtgcata
     6421 caagacgata tgcacggcaa tcgtcgtttt tgccccaaaa gcagttgctt tttttgaata
     6481 aaaggcaaag agaatgatca cgagcagcgg catgttgtat agtccattga attcttgaac
     6541 gaactgatac aatccagccg gtgcaaaaga aatgaatggc gctacgataa tggagataat
     6601 ccccacaacg accgttactg ttttaccagc acgagcgatt tgcttatcat ttgctttctt
     6661 attgataagc ggtttataaa aatccagtga aaacaaggtc gatgtcgcgt tcaaagctcc
     6721 cgcaaatgat gataagatcg cgccaaagat aacagcggcg aaaataccaa agatcatttt
     6781 aggcaatact tcactgacta aaaaaggata cgcgttatct ggatttgaca ttaaactatc
     6841 gccaaaaata ttgcgagcga cgatccctgg aaagacaagg aataacgcac ctaagatttt
     6901 gaagacgccg actaggaaag cgcccttttg tgcctcagct aaattcttgc cggacaatgc
     6961 tttttggacg atcatttgat tggtacacca gaaatacaga ttgttaaaca gcatccctgt
     7021 aaatagtgtc ggccatggaa caacagccga gtcgatcgat cccacagagt taagcagcca
     7081 tggcgtgttt accacaacgt gttcgatccc gccaacaaag ctgccgtcac caagcttcat
     7141 taagcctaaa attgggacca gcagaccaca agccagcaga ccgactccat aaatggagtc
     7201 actattggca cttaacgaca agccgccgat cagcagatag aggatgccga caataccaat
     7261 tgccatcgct acaattatga tcgcgacgat cggctggacg cccagcagct catcaatatg
     7321 gaagatctta ttgaacacca gcgatccaga ataaagaacc acgggtaaga aagaagtcat
     7381 ataggtcaca ataaataaga tcgaaataat tcgtttggta accgtatcat aacgaatctc
     7441 gatgaaatct gagattgtat tgatgccgta tttaaaatat tttggcaaga atatcacagc
     7501 caaagctacg atcgcaatcg tagcagttac ttcccaggcc attacttcca tccctgcatg
     7561 ataactttga ccattttgtc cgactatttg ttcggtggac aggttcgtca tgatgatcgt
     7621 gccggcgatt ggcaaagcag ttaagctccg ccctcccatg aagaaccctt ctgatccaga
     7681 agtatcgaca cttctggatc tagaataggc aaataaccat acacagatga caatcactag
     7741 gaaactgatt atagaaaaaa gattcatcgt ctagctcctt ttcaatagat taaagaccgg
     7801 tgacctctcg aatgtagctt ctggctttga ctgcatattc aaatggattg gctttagccg
     7861 ggtcctgttc tgcttcaact acgatccagc cttcatagtc gctggcatgg attgctgtcc
     7921 agattggagt gaaatcaatc atcccatcac caggaaccgt gaagacgcct ttcttcacgc
     7981 cgtttaagaa gctgtctttt tccttttcta cttcttcagc cgtttcttga cgaatatctt
     8041 tgaaatggat gtgtttgata cgatcttgat gacggttgta aaccgcgatt ggattttcac
     8101 cagaaaagac caaatgtccc gtatcgaaca ataatttaac tttgtctgga tcagtcattt
     8161 ccattaaacg atcgatttct tccgtcgttt gaacgccggt acccatgtga tgatggtaaa
     8221 cgatctccat atccttttcg tgggccaaat cccctaattt ttctaatcct tccgccagtt
     8281 ttttccactc ttcatcagtg aaaactggtt tatcatcaaa gactggcgct gtcataatcc
     8341 cttggatact atgaccctgc tcagctacta cgatgacttt agcgcccatc gcatgtaaga
     8401 aatcccgatg tgctttgaag gcttcggcag tttcttcaaa aggtttagtt gtcagaaacg
     8461 cactgaacca tgcactggca acctttaagc cgcgaatatt caaatattgt tccaacacct
     8521 ctggatcttt tggatatttg ttgccaatct ctgttccggt gaacccagca agggccattt
     8581 cactgataca ttgttcaaag gtattttcac taccaagctc gggcatatca tcattggtcc
     8641 acgcgattgg tgcgatggct aattgaattt gtttggtcat attctcacac tcctgcttat
     8701 ttttttagtt gtactaaact cttcgtatcg aatgattctt gacaagccaa cgcgatctta
     8761 gttgattgca atccatcttc tgcattaacc gctggttgac gtttttcttg aatacatgag
     8821 acaaattctt gggcttcacg aatgaaggct tctctgaatc gttctgggaa gttttgtgaa
     8881 catggacgga tgatcccttg atcgttcatg atcgtcacaa gatttttctc aggcgcttgc
     8941 gctacccgca gcattccttt ggttcccatg atctctgttt cgacatgata gccatgggct
     9001 gcgtttctgc cggcaactag taaagctatt gttttatcgc tcaggctcat cattgcggct
     9061 ccagtttcaa gctcaccagc ctgatccaat tctggatacg ccgcattttt cccaatcgcc
     9121 cacacttgat cgacttcttg gccggtaaac cagcgaacca aatcaatatc atggatcgcc
     9181 atatctaaga acaagccgcc gctgtcgtta ttcatggcaa atttgacaaa gctttccaag
     9241 ccttcgctcg gatcgatccc ataacaacga atcagtgtga tttcgccgat ttcgccatct
     9301 tctaccattt tcttcgcata ctgatacgac tcatcatagc ggcgcatgaa gcctaacatg
     9361 aagacctgtt caggatgctc ttcgatgact ttttgggtct ttttaatatc ttcaacttca
     9421 aggcctaatg gtttttcagt aaagacatgc ttgccatttt ccatcgccaa gcggacttgg
     9481 tcacagtgaa agcctgatgg tgaaacaata aagactgcat cgatcacgga acttttgacc
     9541 atttcttcat agctggaaaa agtatgcttg acgcctaatt tcttctgtgc gaatgctaat
     9601 tctgtttcaa ctaaactgca cgcagccgta agctcacaac aactgacacc tgttgctaaa
     9661 ttttgtgcat gtacttttcc taatcttcct aaaccgacga tgcctactct aatgttgttc
     9721 atgaaatacc tccgttttct gagaattact cattgtatag tgctggtcgt tcatccattt
     9781 gaatcattac ttttgttttt gtttcacgag actcatgaca agccatcatg gtgatcgctg
     9841 ctacataccc atcccaagcg gtaggaccga caaccgcatc atttttgatc gaatcgatcc
     9901 acagcttgaa ttcataatca taggcatcca aaaagcgggt ctgccaatct ggtgaaacag
     9961 gtgtgtaatt tttttcttcc gatttaattc gtgtataggc tgggtccgtc attcctactt
    10021 ctgccgtttc gaaaaccaca tcacatttaa tatcgtagcc aaagcggcaa ttcacaaaga
    10081 tttccaaatc aatcgttaca ccggattttg tttgaaagat cagcagctgc ggatcatgca
    10141 aatcggcatg ggagttgctg gtttttttcg gcaagatcat ttgaacagat tcataatctt
    10201 catttagcaa ccagcgaagc acatcaatct catgaatcgc ggtgttgctg atcgccatcg
    10261 gtgttgtgta gttttcatct gcgctaggat ttctatgggc acaatgcatc attaatgctt
    10321 ctccgtattg cttcgattcg attgcttctt tcagttcaat gtagccgcgg tcatagcggc
    10381 gcatgaagcc aacctgaacc aatttcttcc cataagcaac ttccgcatca acgatccggc
    10441 ggcagcctgc tgcatccatc gctaatggct tttcgcagaa aacatatttc ccatgcttga
    10501 ttgcttctaa cacatactgc tcatgggtcg ggtcccaaga agtcacaaca acgacatcca
    10561 catcttcaga agcgatcaat gcttctccgg tttcaaagaa tttagcatcg acacgtttgg
    10621 caacctcttt agcagctttt acattgatat ctgagactgc tgtcacataa gccccttgtg
    10681 ttttctgatt gatcctgtcg atatgctcga tgccgattgc acctgttccg ataacgccta
    10741 cttttactct caattactta tccccctgtt ctgttgttta agcacacata gtatgcgctt
    10801 acaaaattat tcctgtgtag cttttattct taaaaggtag agccctctct ttgctccgcc
    10861 ttttactacc cataagcttg tacgtataat tccttaattt gactatgact aggcaccttt
    10921 ttattatttt gcggacttcc gctggtcaac gcatccgcgg ccattttgtc taaggctgcc
    10981 ataaacgctt gctgatcaat accataatct cgaatcgttg ggatcgacag ctgctcacag
    11041 agctcttgga tttttttaat cagataatcc gcagcctctt ccagcgataa atcttcggct
    11101 gccggataaa taaatctggc gatcgtcgct aactcagtct gaatagcatc ctttgagaat
    11161 tcaaaaaaga ccggcaataa catcgcattt gatatcccat gaggaatgtg aaaaagtgcc
    11221 cccaccggtc gactcattcc atgaatcaat gtgactgacg cattcgaaaa ggcaatcccc
    11281 gcctccgttg aagctaagct catttgttct ctggcttcag tgtcgtcgct attttcatat
    11341 gcctgagaaa gcgtgttcaa aattttcttc atcgcttcca gtgaaaatag ttgcgttact
    11401 tcattggctt tgactgaaac ataagattcc aaagcgtgac atagcgcatc gactcccgtt
    11461 gccgcggtta cttttggtgg tacagttttc gtaaaatcag agtcaacgat tgagatctgt
    11521 ggcatgaaaa cgagatcctt catcatcatt ttgactgctg tttttgtatt ggtaataact
    11581 gccacatcgg ttacttccga accagttcca gcagttgttg acactgcaat cagcggaatg
    11641 cccgcttgca gctgatcaaa atttcttgca tgaatcgctt cgagacttaa ttgattagca
    11701 gccaaaacgg ctaccacctt ggctgtatcc agacagctgc cgccaccaaa agcaatgata
    11761 tgatcacatt gatgcttctg aaaaagcttc agcgcatcat aaacattttc ttgcgtcggt
    11821 tcggcttgaa tgtctgtata ctccgctaat tgatcatctg tttttcccaa cagctccttg
    11881 atcttttgaa gatgcccggt ctgttccata accgtatcac taatgattaa cggcttgttg
    11941 cctagttgtt ctgattcttc cataagaaat tggattgttc cgccgccaat tcttaaattg
    12001 gcaggcattt tcagttgaat attcacagaa aaaacctctc tctggcttat agcctataac
    12061 agaatcctcg aagctcttta acagagcttc gaagcaaatc tttaaatctc actttgcgca
    12121 taacgtgctg taacaacttt ccgatgggta taaaactcaa cactgtcttt gccattggca
    12181 tgcaaatcac caaagaaaga gtgcttccag cctgaaaagg caaaccaagc catcggtgca
    12241 ggcaccccaa gattgacccc cagcatccca gcatcaacat tttctctgaa gtagcgaatc
    12301 gctgatgcgt tgttggtata cagacacgaa ccattcgcaa attcagattc attcgtcagc
    12361 tcaacggctg ccatcaaatc ttttacccgc acgattgatg cgaccggggc aaaaatctca
    12421 tcctgccaga tcttcatatc catcgtgaca ttatcaaaaa tcgttggtcc aacaaaatag
    12481 cctttttctg gaatgttttc gcgaccatct agtaccagtt tcgctccttc atcgacccct
    12541 ttttggatat agtcaaaggt ccgctgctgt gcatcttttc gaatcactgg tcctaaaaag
    12601 accccttcgt ccaagccatt gccgatcttg atcgcttttg ccgcagcctt aaatttttca
    12661 acaaactgat ccgcgatttc ctcttccaaa acaatgacgg aggctgccat gcagcgttca
    12721 ccagcagaac caaaagctcc gccaataagg tccttgactg cccgatcaat attggcatct
    12781 ttcagcacaa caacatgatt tttcgctcca gtcaacgctt gtacccgttt tagatttttt
    12841 gttccttcac ggtaaacgta ttcaccgaca ttttttgatc ctacaaagct gatgcctttt
    12901 accagtggat cttgcagcaa tgtgttgaca acatcgtggg caccatagac aacattaaaa
    12961 acaccgctag gaaagcctgc tttttcaacc agctcaacaa ttttctccat caacaatggt
    13021 gttttttctg aaggcttcag caccaccgta tttcctgccg ccagagccat cgggaagatc
    13081 cagaagggca ccatcattgg aaaattaaac ggtgtgatcc ctccgattac tccgattggg
    13141 taacgataat tcgtgatctc aatatcagtc gccgctgttg ataaagaatc tcccatcatt
    13201 aaattggtga ttcccgctgc gtgctccaca ttttcgattc ctcgttgaac ttctcccagt
    13261 gactcggaca atgatttccc attttccagc gtgatcaaac gcgccaattc acttttatga
    13321 tctgttaaaa gctgatgcag cttgaacaga taacgtccgc gaacaggaac aggaactttt
    13381 ttccaagttt gaaacgcagc atcggcagct ttaactgctt cggctgtctc tgctttgcta
    13441 gacaacggta cattcgccag cgcttcttct gtagccggat tcattacttc ttcaaactct
    13501 gttgagcgac tagcgaccca acgaccatca atataatttc ttaaaaatct tgtcattta
//
LOCUS       DAJSVF010000067        13520 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSVF010000067 DAJSVF010000000
VERSION     DAJSVF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.070"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..125)
                     /locus_tag="ON550_001991"
     CDS             complement(<1..125)
                     /locus_tag="ON550_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR3955070.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ON550_001992"
     CDS             complement(143..463)
                     /locus_tag="ON550_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955071.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            757..897
                     /locus_tag="ON550_001993"
     CDS             757..897
                     /locus_tag="ON550_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955072.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1195..1452
                     /locus_tag="ON550_001994"
     CDS             1195..1452
                     /locus_tag="ON550_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR3955073.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1423..1731)
                     /locus_tag="ON550_001995"
     CDS             complement(1423..1731)
                     /locus_tag="ON550_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955074.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1809..2555
                     /locus_tag="ON550_001996"
     CDS             1809..2555
                     /locus_tag="ON550_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3955075.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2570..2773
                     /locus_tag="ON550_001997"
     CDS             2570..2773
                     /locus_tag="ON550_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955076.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2878..3177
                     /locus_tag="ON550_001998"
     CDS             2878..3177
                     /locus_tag="ON550_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955077.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKGLSPSDVLKEAS"
     gene            3177..3347
                     /locus_tag="ON550_001999"
     CDS             3177..3347
                     /locus_tag="ON550_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955078.1"
                     /translation="MGKFNRALVFSTPFIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPAGRD"
     gene            complement(3399..3860)
                     /locus_tag="ON550_002000"
     CDS             complement(3399..3860)
                     /locus_tag="ON550_002000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955079.1"
                     /translation="MKKLLYILLFSTFAIVALLLLFEHLKRVNLDGTLLEEQIAILNT
                     PKLLIVFFFMCMLSALIFMFYISTFWVYKEIKNKEFTDKDMIFMGFLVKILFIFSIVI
                     TLLFCLNETQFSVATTLISFIAIFSFIFSDNQKKSINGFLQKIKKKINGEP"
     gene            3914..4639
                     /locus_tag="ON550_002001"
     CDS             3914..4639
                     /locus_tag="ON550_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR3955080.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            4815..5156
                     /locus_tag="ON550_002002"
     CDS             4815..5156
                     /locus_tag="ON550_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955081.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLWKQPRKPEYIYVIEDGLIDALEEYDRDNLIDISYYGQGKK
                     YIAEMEAEFDE"
     gene            5149..5895
                     /locus_tag="ON550_002003"
     CDS             5149..5895
                     /locus_tag="ON550_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR3955082.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPQDVSMLEPASQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            5901..6587
                     /locus_tag="ON550_002004"
     CDS             5901..6587
                     /locus_tag="ON550_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955083.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            6591..7337
                     /locus_tag="ON550_002005"
     CDS             6591..7337
                     /locus_tag="ON550_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955084.1"
                     /translation="MDHRSYYAIIPANVRYDDSLIPSAKLLYGEITALCNEKGYCWAS
                     NEYFANQYGVRKSTIQNWLKSLEEKGYIYREVKYKEGSREIEARYIRILGGGHPEKWV
                     GGHPEIYQDNNTSINNTFNNTKEYIRELPPSKKSKAKPIRHKYGEYKNVLLSDEQMEK
                     LKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRR
                     EKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            7349..7618
                     /locus_tag="ON550_002006"
     CDS             7349..7618
                     /locus_tag="ON550_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955085.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            7624..7788
                     /locus_tag="ON550_002007"
     CDS             7624..7788
                     /locus_tag="ON550_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955086.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECGDG"
     gene            7781..8086
                     /locus_tag="ON550_002008"
     CDS             7781..8086
                     /locus_tag="ON550_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955087.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKTKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSSWIDSKGRQFRYVEEKKC"
     gene            8080..8370
                     /locus_tag="ON550_002009"
     CDS             8080..8370
                     /locus_tag="ON550_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955088.1"
                     /translation="MLDMRIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            8370..8687
                     /locus_tag="ON550_002010"
     CDS             8370..8687
                     /locus_tag="ON550_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955089.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            8684..9499
                     /locus_tag="ON550_002011"
     CDS             8684..9499
                     /locus_tag="ON550_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR3955090.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            9638..9952
                     /locus_tag="ON550_002012"
     CDS             9638..9952
                     /locus_tag="ON550_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3955091.1"
                     /translation="MDLITQYSEIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            10129..10440
                     /locus_tag="ON550_002013"
     CDS             10129..10440
                     /locus_tag="ON550_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR3955092.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEEA
                     E"
     gene            10437..10640
                     /locus_tag="ON550_002014"
     CDS             10437..10640
                     /locus_tag="ON550_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCR3955093.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            10637..10933
                     /locus_tag="ON550_002015"
     CDS             10637..10933
                     /locus_tag="ON550_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955094.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            11010..11426
                     /locus_tag="ON550_002016"
     CDS             11010..11426
                     /locus_tag="ON550_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3955095.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            11685..11837
                     /locus_tag="ON550_002017"
     CDS             11685..11837
                     /locus_tag="ON550_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955096.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(11834..12109)
                     /locus_tag="ON550_002018"
     CDS             complement(11834..12109)
                     /locus_tag="ON550_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955097.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            12563..12769
                     /locus_tag="ON550_002019"
     CDS             12563..12769
                     /locus_tag="ON550_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955098.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            12965..13132
                     /locus_tag="ON550_002020"
     CDS             12965..13132
                     /locus_tag="ON550_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3955099.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            13158..13502
                     /locus_tag="ON550_002021"
     CDS             13158..13502
                     /locus_tag="ON550_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3955100.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         4959 a         2111 c         2795 g         3655 t
ORIGIN      
        1 ccatatagat tttcattcaa aaaaatgatt ctaggcttcg gataatagaa gccaggctct
       61 ttcatttcca tgtaaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaac ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtttt
      241 ctgataaatc cacatgttta ggggtttctt tttcttcgcg ccccagaaga tagtctacag
      301 agacatcgaa atagtcagca atttcttgca aacgttctgt agaggggttt gagttcttaa
      361 gtctatataa gacgttttta ccatagccta ggtcttcttc aactttattt aatgattttc
      421 cctgtttttt cgctaactct tttattctct caaacgttat catatcaata ttcctttcgt
      481 tcaagaaaaa atatttaact ttatttgtta aaattcgttg actattttaa caaaaggtgt
      541 taatatcatt cttgtaaaca agtttaacaa ctaaaaagac aacaaaaaac actattgatt
      601 aataaatgcc aaccgccaag aaagctttta aaatcaatgt ttatatgtct tatttaacta
      661 tgttctgatt ttaacactgt atgttaattt agtcaacgca gttttttaaa aagttgttaa
      721 atttgtttac gaatataaaa gaaaggagag aagaatatgg aaaaaacagt ctcggcaaaa
      781 atcgaagatt tgaaattaga catactgaaa caagcaaaag tggcgatgga acacgcggta
      841 gataaagaag actctgccat ggttgcagcc atagcagaga ttttagctca cgtttagtca
      901 tcgtttttac ggtcttctgg aagctgacca tagataacag cataatgaat atctaaataa
      961 gcttgaacaa tttcttttgg cgaaattgat tcgcctttag tagatacttg tgattcgtga
     1021 tttagccatg caacaactaa atccgcagcg atttcaaacg gcgttacact ttgtgtcagt
     1081 tgccataaag aatttcacaa tatgtatggt cgtggtaata ataccaaaga gcaatttgag
     1141 gaatttatgg aaattaaaaa atacaaaaaa tcatttatta aaggaggcta aacaatgaac
     1201 aaggaaatga gccaaacaat caaggtacaa aaaatgatag atgatctaac acatggaatt
     1261 gatagccaag ccgataaaat catcaaggaa ttgcaaggac aaaaagttaa ggatgcaaaa
     1321 atgcttctca agactatcaa ctttgaaatg aatccaacaa aaagaaagct cgccgatgtg
     1381 ttggaagaaa agttggcttc cgctatcaac gagcaagaat tactatttga aaccgatact
     1441 tttaacagct gatgttttat agatgacagt ttcatccgaa tctatatcat cgccgagaga
     1501 aaagaatgtt gcttttgaca aaaagccgat aagacctaca agcggtcttg atgttgcaag
     1561 agtagatacc ccaatatcac cttgcaaggt attcacgtaa atttgagaag aatagaacat
     1621 tccatctttt tcttcgatat ttgacgcatc atcgtatcca acgaccaaag tatcggcaaa
     1681 tattttgatt ttttctccat caagcatagt aattacaaat tctggtttca taaattttca
     1741 cctccttaac aattatttca gcctgtcaca ctgataagga aattatacca aagaaaggaa
     1801 tgagaaatat gaatacacca caaattttta atttcgagca aaacgaagtt cgaactattt
     1861 tagtaaatga tgaaccatat tttgtaggaa aagatgtagc agacgttcta ggatattcga
     1921 atccgcaaaa agctattcga gatcatgtgg acttggagga taagacgcag aacgattcgt
     1981 tcaccgtcaa tggaacagca gttgttttga ttaacgagtc aggcctttac agtttaatct
     2041 taaaatcaaa acttccctct gccaaaaaat ttaaacgttg ggtaacgagt gaagtccttc
     2101 caacaattag aaaaacaggt agctattcaa acgtacctca aagttttgca caagcattgc
     2161 gtttagcagc ggatttagaa gaaaagaacc aattactcga acaacaaatt gccgagtacg
     2221 aaccaaagat tagctactta gatacgattc tttcatcgac agatacggta gctacttctc
     2281 aaattgcagc tgattacgga atgtcggcaa ttgctctaaa caaattgcta aacgagttag
     2341 gtgttcagca taaagttagc ggacaatgga tactttaccg aaaacatatg aaccaaggat
     2401 acacaaaatc gcacacaagt gagataccga aagccgatgg cggcactaaa gttgtaatga
     2461 ataccaaatg gacacagaaa gggcgagtgt ttatttacaa cttattaatc gcagagggct
     2521 attaccctca aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat
     2581 tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt
     2641 cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat
     2701 tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat
     2761 gtacgaattg tagagggagg taaagtcgtt ggaagataaa atcattctta caaaatcaga
     2821 gcttcaagaa atgttaaaca tcgagtatgg aacgataatt tttaggaggg ataataagtg
     2881 aaaaaaccaa cactttcaga gctgatagaa gctgctgaga aggcagtaaa accagacgac
     2941 tggtaccgac aaagtttaat cttggagaag ttccacggca tgtcaaaaac tactttagtt
     3001 gaatactgca aggaaatgga aacaattcct gaatttgcag aaggaatact tcgtccaggt
     3061 cactcaacca catttattca ttaccatact tttatttggt ttttaaagtg gaaagacgca
     3121 aacaaatatc gtgtaaaagg tttatcgcct tcagatgttt tgaaggaggc aagctaatgg
     3181 gcaaattcaa cagagcgcta gtattcagca caccattcat catctacgct ttaggtcttt
     3241 ggggaagcag acaagcatta atcggcacaa tcgtatatat ggtctggatt tttatggggc
     3301 ttgatgaagc tgagtacaga gcgaaaaagc cagccgggag ggactgacta atggtcttcc
     3361 taagaggagt aatcaatatt tttttcctga tttttatatt agggttctcc attgattttt
     3421 ttctttattt tttgcagaaa tccgttaata ctttttttct gattatcgga aaatataaag
     3481 gaaaaaatag caataaatga aatcaaagtg gtcgctacag aaaattgtgt ttcatttaaa
     3541 caaaataata aagtgattac tatggaaaat ataaaaagaa ttttgactaa aaaacccatg
     3601 aaaatcatat ctttatcagt aaactcttta tttttaatct ccttgtatac ccaaaaagtt
     3661 gatatataga acataaatat cagagcggat aacatacaca taaaaaagaa tacaattagt
     3721 aatttgggag tatttagaat cgcaatttgc tcttctaaca aagttccatc caagtttact
     3781 cttttaagat gttcgaaaag caacaataat gcaacaattg caaaggttga aaaaagtaaa
     3841 atatatagca attttttcaa atattttcac caccattttt tacctaaatt atatcaaaag
     3901 gagagaagaa aaaatgcaag aattagtaat tttgaaaaat aaagaagctg taactacgag
     3961 cttacaagtc gcagacagtt ttgaaaagaa acacaagcat gtgctagaag caatcgaatc
     4021 aataaaaaga tcggtcgaaa attcggccaa tgttgaagat gggtccaatt ttggacagat
     4081 gtttgtggaa gggaacgagc cagactcata cggaagaagt cggagagttt atttcatgaa
     4141 tagagacgga ttttccttgc tagctatggg attcactgga agtaaagcaa taaatttcaa
     4201 actaaaattt attgaagctt tcaacgaaat ggaagatgtt attcggaaga atactgttcc
     4261 tcaaacaatt gaagacatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga
     4321 tgtttccatg cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca
     4381 aaaaggaaat atgaaagtta tggaagttct agggggaaaa gaaagccgag cttatgaaga
     4441 aatcagcaaa aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc
     4501 aagatatggc gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg
     4561 gttaccagaa actgcgatcc gcatggaaat cgatcaactg aacagacaac aaagactttt
     4621 cggtgatgaa aatgaataga gctgaagcgc taaaaatagg cgtaataatt gctaatcgct
     4681 ggtggagaca caataaacca aacatcctaa gccaacaaca tattgataag caaaaagcat
     4741 ggcaacaaat aaaaagcgac tccgccggca agcattgagt cgcaaacaaa atacatctaa
     4801 ggagatgtta ccacatgaaa aaagaacttt ccactctaga tcaatatttg actgatccta
     4861 gttggggcaa atcgaatatc aaggaaacaa gcaatcgaaa aatcagacgt aatcttttga
     4921 cggatgaaga actagcatgt gatcaagacg atttgggaaa ttttgtgagt atttggggac
     4981 atgtctacct tatccatcta tggaagcagc caagaaaacc tgaatacatc tatgtcatcg
     5041 aagatggctt gattgatgca ctagaagagt acgacagaga taacttgatt gatatctctt
     5101 attacggaca aggcaaaaaa tacatcgctg aaatggaggc agaatttgat gagtgaaaag
     5161 gaacaacctt tgaaaaatag aagtgataat acgcttttta ataccttata caaaataaat
     5221 gtgaaagatg ttactgaaaa acgaaataac ctcacttatc tttcgtgggc atgggcttgg
     5281 gcagaagtta gcaaagtgtg tgaggcagta gattatgaaa tctatcatga tccagaaaca
     5341 tatctgccgt atgtctttga taagaaaaca gggtacatgg tttttaccag tatcacagtc
     5401 aacggagtaa agcgtgacat gtggttacca gtcatggatg gtgcaaacaa ggcaatgaaa
     5461 gatgagccat atacctacga agtcaatgat tatcagtgga ataacgaaac gaagaaaaaa
     5521 gagattgttg gaaaaatcga aaagcgagtt gaagcagcaa ctatgtttga tatcaacaaa
     5581 acgatcatgc gctgtcttgt aaaaaatcta gcaatgtttg ggctagggct atatatattt
     5641 gctggcgaag atatgccaca agatgtctcg atgcttgaac cagctagcca aagaagcaaa
     5701 aagctattct tagatgcttt acaactggtt gctaacaagt acgaaaaatc gattgatgaa
     5761 gcaattgttg cattgactga tgcagcttcc ataaccgccg atgacagtaa atggaccaag
     5821 agagacttgg gcattctaaa acgaggcgtt aactggattg aagatcagta cagagaagaa
     5881 acaaaagaga agtgatatga gtgtttaaac cattaatcga ttcatattca gcggttctga
     5941 aaaagttcaa aggaaaagac ataggtgcaa cgatcaatga agaagtgaac attgatcgac
     6001 taaagacgat gtatgacggc tacgatggcg atcgagtcat tgaaattcgt tttattgatc
     6061 ctagacgttt caccgtacag caacgaaact tcatctatgc gctgataggc gatattttta
     6121 tcgatacagg catgccaacg gacttctgga aggaattctt ctacttccgt tttgaaggcg
     6181 tcacagggcg caaaataagc ctcaaagacg aatcgaatac gactgtgagt gatgctaatg
     6241 tcttagcaaa tatcatttta gatttcatct ttgaacacca tattcctttc aaagaaggct
     6301 atgagatttt accagcgaat caagagtatt acttctacaa atgcatcaca aaaagagtct
     6361 gctgcatctg tggcaaaaca ggagctgata ttgatcactt tgacaaagct ttaggaagac
     6421 gaaagcgcaa agaagttgat cattcagagt acacatttgc agcactctgc agaatccatc
     6481 acacagagaa gcacaagata ggtgtgatca atttcaaaaa taagtatcaa atcaaaggga
     6541 tcaagttaaa ccaggaaaca atcaagaaat taagaatcgg aggatgaaaa gtggaccaca
     6601 gaagttatta cgctatcata cctgcaaatg ttaggtatga cgattctttg atacctagtg
     6661 caaaacttct ttatggagaa atcacagctc tatgtaatga gaaaggttat tgctgggcta
     6721 gcaatgagta ctttgccaat cagtacggtg taaggaaatc aacgattcaa aattggctga
     6781 aatctcttga agaaaaaggc tatatctatc gagaagtgaa gtacaaagag ggtagtagag
     6841 aaatcgaggc taggtatatc agaattttgg gtgggggtca cccagaaaaa tgggtggggg
     6901 gtcacccaga aatctatcaa gataataata catctattaa taatacattt aataatacaa
     6961 aagaatatat aagagagtta ccgccttcga aaaaatcgaa ggctaagccc atccgtcaca
     7021 aatacggaga gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaatag
     7081 aattccctaa tgactatcaa gaacgaatag aacggctatc tgagtattgt gaatcatctg
     7141 gtaagactta taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg
     7201 aacctaagaa cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat
     7261 gggcaatcga ccaagaagcc tatcttaaga aaaaagcgct agaacgagct aatagacaat
     7321 caaaagcacc attctaagag gtggaaaatt gaagatcgat tatctagaac taattaatga
     7381 aatagcaaag tataaaactg gtgaggaaat agaaattctg agagacgtat atgatcaact
     7441 cgaagaagct ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata
     7501 ctatttcgca ctttatatcg atggaacaca attaagaaat ttagcatata ccaaattact
     7561 atttgttgat tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaaac
     7621 aagatggacc taaagacatt tacagcacag atcgaattaa tgcatcaaga agctttaaga
     7681 caaagtgtat cgtacgaaga caagtggctc aacacgtttc atggcggacg tgagagcgca
     7741 cttgatcaag tactcaaatt attgaaagga gaatgtgggg atggataaaa aagcggcaat
     7801 gaaacgaatt gctgaattaa ccaagtcaga atcttggcaa gaagacaaag aaatagttgc
     7861 agaagtccaa aaactcggca aatcaatgtg gactgaaaaa accaaacgga aaacgccgag
     7921 aaagattgca atctggcatg gtgatcgaat tctagtaaca ggtactgctg aacagttatc
     7981 tgaaattact ggattaagca aaaacattat ttgggataga gctaggagct catggattga
     8041 ttcaaaagga cgacagttta ggtatgtgga ggagaaaaaa tgctagacat gagaatcgaa
     8101 gattatcgaa ttaccagtga ttctagaaac attgtcctat caaaggtaag acgagatgag
     8161 gaaggaaata tccgctacac agaagcaaaa gaagaatcac gagcagatat cggatacttc
     8221 caaactgtct catcgtgttt gaaggcgata caacgcgatt acgtgttaag tgaagaaaga
     8281 acgataaaaa gtattatcga gtacaaaaaa gcgttagaaa acatcactag acagtttgaa
     8341 caggcatgtg agattgagga ggagaaataa tggaattcgt agacttaaat gtacgtggga
     8401 tcaaatgcga taatccagaa tgtgattatt ctgatatggc agtaaaatac gaaaattatc
     8461 cgcaatggtt gaacaagcca tgtccgaaat gcggcgctaa tcttctgacg caagaagact
     8521 tggatgcaac agagcagctt atggagattg tcaatcttac aaacgagatc ctcaaagatt
     8581 caggattgga gaagcaggat atgaataaat atatcgtacc tgtagaagcg aatggaaccg
     8641 gtgaattgtc atttggagaa ataaaaaaac tggaggaaga aaaatgaacg aaaataaatt
     8701 aatcaaacta ggtgtggcag gagtagtaat agtaggtatt ggagttatcg gaggatttaa
     8761 gtttttcgaa aaaattgata acggatatgt gggtgtgcgc tattcaatga atggcggtat
     8821 caaagatgaa gcactgacgc aaggtgtgaa atttgttggg attgacaaag tgatccaata
     8881 tccaattcgc ttgcaaacta tccaatcaaa aaacatttca gtatcaacaa gcgatggcaa
     8941 aaagacaacg attgatatca aatatgacta caaagttgat tcaactaaag cagcaaaaat
     9001 gtacaaagaa tttgggaata taacttcgga agatatcgaa agtggatggt taaaatctaa
     9061 gcttcaaaaa gtagcgcgtg aagtttatgc caaatatagc ctgcttgatg tcctttcagg
     9121 agattcctct aaagttgaag ctgaggtact aacgaacttt gctaaatcag ttgaatctaa
     9181 agggtttgaa gtcgaagacg taacacttgg tgttccagat gtcgataaag aaacacaaaa
     9241 atcaattgat gcgatcattc gagctggtca agaaaatgaa aaagcgaagc tagatgcaga
     9301 aactgcaaaa actcaagctg atagtgaagc ttacaagaaa acaaaagctg cagaagcgga
     9361 agctgaatca aaccgtaaag ttgccgaatc tgtaacagat aatttaattc gttatgaaga
     9421 agctcaagct cgaaaagagc atggatgggt aacagtaaac ggagcagata ctgtagttac
     9481 ggatgaaaca ggtaaataat atgggattct ttatagctaa aattctcttg ttcttaggtt
     9541 tggtaggagt ggcatatctc gtgtatgccc tcctttccaa aactgatgac aaagaagatg
     9601 acaacgacga tgaaatgaaa ttttaggagg agaaataatg gatctcatta cacaatacag
     9661 tgaaatcatc ctcaagaaaa tcatgatgaa gattcagaaa gataaaaaat caaaagaacg
     9721 agcggaatta gttaagttgg aaatggctga aacaggagca ggagtgcgaa gtagcaggca
     9781 ttggaaagca gcagcaaaca ttgaattcta ttacaacgaa attcaaaaag ggttcgatca
     9841 gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa cttcatcaag atcgtttcaa
     9901 atttgtagaa aagtatagag agatactgaa tgagtatttc gaggaggact agatgtttaa
     9961 tttaatgtta gcgtcaattc actttttatt ctatataacg atatttgtag ctatattaat
    10021 taatacgata aaaagcggtt ttttaacacc aatcggcaca attttaacat tagcttcatt
    10081 tgttacaagt tgtattatcc aacttaaata tataaaggag aatgaataat ggacgaacta
    10141 atcacaaaag tagagcagtg ggctaaagat aagggattgg atcaagctga ttccagcaag
    10201 caaatgttaa aaacgatcga agagattggg gaagttgccg cttctctagc tagaaaagat
    10261 gaacatggtt taagagacgg aattggagac gtagtagtaa ccttgattat tttagctatg
    10321 caaaatgata tggatttgta cgagtgtctg aaccaagcat acaacgaaat caaaggacgc
    10381 acagggaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga agcggaatga
    10441 acaacagaca tcgcagaata acaaaactaa gaaaacagga actgaatgta ctaaagacaa
    10501 agtttgaaaa agaatatgga atttcagcag aagaaacata taaagtggca agtcagtgtg
    10561 ttgctgatgc aagtgatgct attcgtaagt ttggaatttc gatattaagt gatgatcgta
    10621 aatgggagga aaaagaatga aactaaaaga cggattttac gctagcagtc acggtatcgg
    10681 cggtttaatg ctagatatgc cgacaaagaa tcctaaaaca cgtaagaaac caaaagtcaa
    10741 agtcggtgac atggtccgct gtgaagcaga ggagttcatt tatccatttc gtggatatgt
    10801 agaacacgtc tataatcact cagcgatcat tcgcattgaa aacacgatgg aatgtgataa
    10861 atggacagct aaaagcaaag agaatttagc agtagtgaga ttggtggata tggaactaat
    10921 caatgacaaa taaaaaagcc ggatcgctcc gactaacata ataaaacaga caagtttatt
    10981 atatcacata aaaggagcag tttgacttga tgcaattgtt acgagaggta gatttcaaac
    11041 agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag cggatggcag
    11101 gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag gcaccgaagc
    11161 acggcaataa ggcagaggat gcattgattc agatgatgga tatagaagcg gagagagacg
    11221 cgattttagc ggctttgatg gctcttagtc tgattagtcg tcagatactc tactatagct
    11281 tctgtgacgt aaataagcac tctaattatg aaataggtca attgatacga ggatacggag
    11341 agaagaatgt agagaagctg aaatctatcg cgctgatcga atttgctgaa gcatacaaaa
    11401 aaggcgtgtt agttcagtat cgttgatttt gtagggtttt tgtagggata gtgtagggtt
    11461 tttgagtgtt ttaacgtgat attatgatag tgtcgaaaga ttaggaaaca ggatcgacaa
    11521 aataaaatgt aagggaggga atctccctca tcgttttaaa ttaagcttcg ataggcagca
    11581 acggaaatat taagaataag gatgtgaatt tcaactcctc ctgattgttc ttattatcta
    11641 tcatccgttg ctgtctatta atttatgtat tggagggaag aaaaatgaaa ttatcagtag
    11701 aaggcactcc agaagaaata caagaattgc tccaagctat tggtagtagc aaggagcaaa
    11761 aggtaagtgt ttctaattca gatattgacg gtatttgcaa ttgtttaaac agagaaaaaa
    11821 tatctaatcc ttactaagag aaaatatgtt cgttaatgaa tttccaattt tcctctgtag
    11881 ctctaccttg ataatttttt tgcagttcac agatgaataa ggaatcgtta ttatcaagta
    11941 ctgtatttaa tttttcacac atttgatttg gattcaaatt agttttaact aaccaaaaag
    12001 atttttgtag ctttatatat gccccaaatt cttttattat ttcaaatact ttatcatact
    12061 tttgccctgg gttattaaga tcataactaa tcatgtaggg tttatacatg ttgtcttgca
    12121 catccttgtt ttatatttca gcggaccact cgctgataaa taaaattata cgcctagtat
    12181 ttattttcac aatattaatt tatcgtaata acttttgagt aaatagaaca aaaaacctgc
    12241 actagtttcc gctagtacag gcagtgacta tatcggttga taatctagca tatattgaaa
    12301 ataattgcaa gaaaagaaat ttatagtggt ttataccaaa ttatcaaagc aatgttattt
    12361 tgttgctgtc tattgttttt taattattca cacgataact aaaggtgggt gaagagaaat
    12421 gattccatta ataatttcaa tttttgcgct ctgtcttaat gtctatatga ttggatttaa
    12481 aaatgggcaa aataaaaaat agtagcagcc aagaataatt ttatagtgtc actgtggcgg
    12541 aaagggtaga cgctaagcat gtgtgctagg tcaatgcttc ggcaaccatg caaggttcga
    12601 ttccttgcca gcgacattaa atgcctatga cggttacgac taccgaaaaa agatcgttaa
    12661 gaagctatac ggtgctacgt acggcaatgt agtaagtgtg ctatctgtac accaccaagc
    12721 ttcggtcact gtggcggaag tagaagacgc agcggtaaat ggcgagtagc ctcgtgagag
    12781 cctggtaagt tctcgtgagt ggtgcaatcc cactccagcg actttaagca accgaggcat
    12841 cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat cgtgcaagtt
    12901 actattacat attagatcac tctttgagtg gtctttttat ttttgcacaa aggaggaaac
    12961 aacaatgtat agaccacaat acttagaaca gaagtatgaa gtaatcactg ttcataaagg
    13021 taatggcgaa aaagtatatg agtatagaag accaataaag agcgatacat ataaacgaaa
    13081 ggaaaacaat gaagttattc cattgtatgg caaaagaata gctaagcatt aaataagatt
    13141 gcgaaaggag acggaacatg accgaggaat tctatagatg gctattacag ttgacaagag
    13201 aagatcgttt ggttaagttc tatcagtctc ctaaatggcg caggcttaga gagaaagcga
    13261 tgaaacgaga tcactatgaa tgccaagagt gtagaagact aggtaagtat catagagtag
    13321 agaacgttca tcatataaag gaagtcaagg atagacctga cttagcttta gatttagata
    13381 atcttatttg tttatgtgtt gaacatcata atgaagttca tggcagatat cttacagcat
    13441 tagataaaca agagaagaag atagaaagct ttgctaactt cgatgcaagt gaaaggtggt
    13501 aagtgcatga tcatcaatga
//
LOCUS       DAJSVF010000068        13429 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSVF010000068 DAJSVF010000000
VERSION     DAJSVF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.071"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..914)
                     /locus_tag="ON550_002022"
     CDS             complement(<1..914)
                     /locus_tag="ON550_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR3955101.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ON550_002023"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ON550_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3955102.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ON550_002024"
     CDS             2006..2176
                     /locus_tag="ON550_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955103.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ON550_002025"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ON550_002025"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR3955104.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ON550_002026"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ON550_002026"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR3955105.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="ON550_002027"
     CDS             4440..5321
                     /locus_tag="ON550_002027"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR3955106.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="ON550_002028"
     CDS             5318..6034
                     /locus_tag="ON550_002028"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR3955107.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ON550_002029"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ON550_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR3955108.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ON550_002030"
     CDS             6751..7752
                     /locus_tag="ON550_002030"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3955109.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ON550_002031"
     CDS             7749..9110
                     /locus_tag="ON550_002031"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR3955110.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ON550_002032"
     CDS             9070..10221
                     /locus_tag="ON550_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR3955111.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ON550_002033"
     CDS             10196..11365
                     /locus_tag="ON550_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3955112.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGVVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ON550_002034"
     CDS             11540..12919
                     /locus_tag="ON550_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3955113.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ON550_002035"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ON550_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2925 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag gggttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAJSVF010000069        13356 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSVF010000069 DAJSVF010000000
VERSION     DAJSVF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.072"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            230..472
                     /locus_tag="ON550_002036"
     CDS             230..472
                     /locus_tag="ON550_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HCR3955114.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            469..939
                     /locus_tag="ON550_002037"
     CDS             469..939
                     /locus_tag="ON550_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3955115.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1067..2281
                     /locus_tag="ON550_002038"
     CDS             1067..2281
                     /locus_tag="ON550_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3955116.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3270..3479
                     /locus_tag="ON550_002039"
     CDS             3270..3479
                     /locus_tag="ON550_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HCR3955117.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3591..5204
                     /locus_tag="ON550_002040"
     CDS             3591..5204
                     /locus_tag="ON550_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955118.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5239..6450
                     /locus_tag="ON550_002041"
                     /pseudo
     CDS             5239..6450
                     /locus_tag="ON550_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6473..7399
                     /gene="glsA"
                     /locus_tag="ON550_002042"
     CDS             6473..7399
                     /gene="glsA"
                     /locus_tag="ON550_002042"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR3955119.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7607..9067
                     /locus_tag="ON550_002043"
     CDS             7607..9067
                     /locus_tag="ON550_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955120.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9288..11051
                     /locus_tag="ON550_002044"
     CDS             9288..11051
                     /locus_tag="ON550_002044"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR3955121.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11135..11593
                     /locus_tag="ON550_002045"
     CDS             11135..11593
                     /locus_tag="ON550_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR3955122.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11702..12385
                     /locus_tag="ON550_002046"
     CDS             11702..12385
                     /locus_tag="ON550_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3955123.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12532..13146
                     /locus_tag="ON550_002047"
     CDS             12532..13146
                     /locus_tag="ON550_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR3955124.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13162..>13356
                     /locus_tag="ON550_002048"
     CDS             13162..>13356
                     /locus_tag="ON550_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955125.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         4636 a         2110 c         2798 g         3812 t
ORIGIN      
        1 aattgattgt cgttttcttt tgcagaaatt taagtgcagg caattatggt agttgtcgta
       61 attgctgtca gctcagtgtt tatcactgat ggtgtgaaag atttacggtt ggagatgttc
      121 aataagatta ttggtgctta attattttta ttgtagtgct gatatgagaa tgttataatt
      181 ggggtataaa acatacaaat aaatttgctg aaaaatgagg tatcaaaata tggaaaatga
      241 attactggaa aacttaggaa gacttcacac aactgaactt ggcgttatta gaattaaaaa
      301 gaacttatct ttgaatgtag aaaatgtaat tgaatggtgc aaagaaaaaa ttagcctttc
      361 taatgcggaa atcacaagaa aaggaaaaaa ctggtatata acgattgata attgtattat
      421 aacggtaaat gcgtacagtt ataccgttat cactgctcac aaggtaaaat gataaatttt
      481 tctataaagg aattaaacgc atttgcagtt attgggcagg aagtagaact gactaattat
      541 caaaaaaaga atattcagat tagtacacag ttttggagaa agtttaacag tagtttgaaa
      601 aaagcgtatc tttcacaatc aggaaattgg gtaaaatatg cttttatgga aagaagaaat
      661 ggaaaacttt attatttctg ttctattccc caaagaacca ttaccccaga gggctttctg
      721 tataaagaaa taccgtctta taaatatttg gttgtggagc atattggtgc tatggataaa
      781 atatatgaaa cttatggcaa gatttatgaa gaaattattc ctagtacatc gtatattcct
      841 ataaaagata ttatcctaca ctttgaaaaa tacgattatc gttttcattg gaatagtgat
      901 aattcagtta ttgaaatttg gatacctatt caagattaga agcaatttta tatccataca
      961 cacaaagcag ttagtcttag gattgactgc tttttctata cagattttta atgacaggca
     1021 attatcaatt atggtagttg ccttttttga ttggaggaat tgaagaatga atgataaaaa
     1081 ctttattgaa gaactaagac aaaagcgtga ggaatacgga gtaacacaaa caaggcttgc
     1141 tgttgcctgt ggtatcagcc gtgaatatta caaccgcatt gaaaaaggaa aacagccatt
     1201 gaatgacgaa ttaagagaaa tcatagaaaa acagattgag cgtttcaatc cgcaagaacc
     1261 actattctta ttgattgatt actttcgtgt ccgctttcct actacggacg cattggcgat
     1321 tatccgtgat gtattacaac tgaaatctaa ttacatgctt tatgaagatt atggaaaata
     1381 cggatatgaa agcaaatatg tacttggcga catcaatatc atgtgttcca tgcaggagca
     1441 tttaggtgtt ttattggaac tgaaaggcaa aggctgtcgg caaatggaat gttatctgct
     1501 ggcacaggaa cgctcatggt atgacttcat gctggattgt atgacggctg gtggtgtgat
     1561 gaaacggctt gatttggcta tcaatgataa agcaggaata ttggatattc caaagttaaa
     1621 ggaaaaatac aaggctggcg aatgtatgtc ctacttccgt atgcagaaag attacagcgg
     1681 tacagaaaaa tgcggtagcg atttaccaaa gaatacagga gaaaccttat atctcggttc
     1741 aacaagtagc gaattatata tgtgtgctta tcagaaaaat tatgagcagt atgtcaagaa
     1801 tggcacagaa attgaagata cggagattaa gaaccgtttt gaaatacgca tgaagaacga
     1861 acgagcctat tatgcggttg tagatttact gacctatcgg gacgctgaac gcaccgcttt
     1921 ttctatcatc aatcattatg tccgctttgt tgacagagag gacgacaagc caaaaagtca
     1981 atggataaca aatgatgatt gggcatggtt tgtaggggaa aacagagagc cgatacgctt
     2041 aaccacaaag ccagagcctt acactttaca aaaagcgttg cattggctac aaagacaagt
     2101 cgcaccaacc ataaaaatgg tacaggcatt agacagagaa aatcgtacaa cgatactgaa
     2161 agatatgatt gagcaggcag aactaaaaga caagcataaa catttattac aattagagaa
     2221 atcaaccata gaagaacgta tagataccgc tgttccacaa gagaatgacg gtatttttta
     2281 atgtaattga aaaatttcag atttagtcag caaagagctg ttttcattcc ctatgtgaag
     2341 tgaaagaaga aaaataaatt ttttattaaa tctgcaacaa aatggctact tgcgtacaga
     2401 tatggtgcga gaaaaggaaa tctaaacttt ttttgaaaat aggtcattaa atcggggctt
     2461 gctgtcccta tatgaagtga aagaagaaaa aagttttgaa aaaatgacgg aaattcgtga
     2521 ttagtggaca gtcttctgtg aaaagaggaa aaacatcatc attttgtatt caaacaggca
     2581 ttagcagttg agtgttgatg tgaaaagatg aaaatacttt gttctcaact taacaattta
     2641 aggtttccgt tccctatatg gtgcaaagaa aatatttcat tctattgttg gcagtaacgg
     2701 tatggtttgg gtagttaggg tgtgaaaagg aaaatattct tttttcatca tcaacttagc
     2761 aaaatgacat tttctatccc tatatggtgc atgaaaagaa gttaaaattt ttccatttaa
     2821 acttagcaaa tggacgtttg ctgtacagat agtagtgaaa aaggagaaaa tcaaccgttg
     2881 attgccaaaa tgaaccgttc catttgtact aataatggaa aaagttttgg attttggtta
     2941 cgtttctcct cttttccaac gcgatatata ggaaagacga tttttttctt tcaatcgttc
     3001 tttgacaact gaataaagct attcaatacg tttgacagac agctagccgt tgaaacaaat
     3061 acgccacgac ctttcccctg caagagcgag cgaaaaccta tttgtgttct tgtaaaagat
     3121 ttgtcctctt ttatcgccat gacctgtact gtcagataat gatactcccg tacagccaca
     3181 gcttgagcat taagagcgtc gcacgcaatg ggtacggcta cataagaact atgcagaggt
     3241 ggaactcctg tgggctatga caaaagccgt tgaattgctt agaaagattt tacagaaagg
     3301 gggtatgtgt tattgataat gctgtaaaaa caattcaaaa gaaaaatggc aagtgtggca
     3361 ttggcaacag aggaaagaca aagattgtag taaaagagca tttttccgaa aagggcaaaa
     3421 caatggaaga acttctaact gatgtcatgc tggaaaaagc aaagcagaca atcgcataaa
     3481 atcggtgcag ttgtaagaaa tagattgaat gacaaaaagt acccgtgtta tactttactt
     3541 gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca atgaaacaac
     3601 agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta cagggcgaaa
     3661 gttccagcat taccacacag agaagtatgt tgcgtttata tgcaaaagaa catcatttga
     3721 atgtcattga tgaatatatt gatgacggct ggtcgggaac aaattttgac agaccgagtt
     3781 ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta acgaaagacc
     3841 tttcccgtct tggcagaaat tatattatga caggacaata tacagaattg tatttcccta
     3901 gtcataatgt ccgctacata gcgattgatg acggtgtaga cagcgaaaaa ggcgaaagtg
     3961 agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca agccgtaaag
     4021 tcaaatcagc atttaagaca aagtttgcgg agggggctca ttacggtgct tatgctccgt
     4081 taggatataa gaaacaccct gacatcaaag gaaaactgtt ggttgatgat gaaacaaaat
     4141 ggattattga aaaaatcttt tccctagcct atcaaggtta cggaagtgca aaaatcacaa
     4201 agcagttaag agcagaaaaa gttccgacag cgtcatggct aaattttaca agatacggta
     4261 cttttgcaca tatctttgag ggaaaacccg aaagtaagcg ttatgagtgg acgattgctc
     4321 atgtcaaggc gatattgaaa agtgaggttt atatcggaaa cagtgttcac aatatgcagt
     4381 ctacggtatc gttcaagagc aaaaagaaag tgcgtaagcc cgaaagtgaa tggtttcgag
     4441 tagaaaacac tcacgagccg attattgaca aggaagtgtt ctatcgtgtg caggagcaga
     4501 taaaatcaag acgcagacag acaaaggaaa aggcaacgcc gatttttgca gggcttgtca
     4561 agtgtgcgga ttgtggttgg tctatgagat ttggaacgaa taagacaaat aaaacgcctt
     4621 acagttatta tgcttgcagt tactacgggc agtttggcaa aggtaattgt tctatgcact
     4681 acattcgtta tgatgtgctg tatcaagccg tattggaacg cttgcagtat tgggctaagg
     4741 cagtacagca ggacgaagaa aaggtattga acaagataca gaaagctggc aatgcagaga
     4801 gaatacggga aaagaagaaa aaggcaagta cattgaagaa agccgagaac agacaaaatg
     4861 agattgaccg tttatttgcg aaaatgtatg aagatagagc ctgtgagaag ataacagagc
     4921 gaaattttgt gatgttgtcc agcaagtacc aaaaagaaca gatagaactg gaacagcaga
     4981 taacaagcct aagagaagaa ctaagtaaaa tggaacagga tatgataggt gctgaaaagt
     5041 ggattgagtt aatcaaggaa tattccgtac caaaggaact gacagcacca ttattaaatg
     5101 ccatgataga aaaaatcctc atacatgaag caacaacgaa tgaggataac gaaagaatac
     5161 aggaaattga gatatattac cgatttattg gaagagttga gtaatcaaca agagtaatat
     5221 ttttaactaa gggaaaccgg cggtgtctat acttgggtca gtcgcacatt gggaaaacga
     5281 ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta cttgaccatg
     5341 ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat acaaaacaat
     5401 aaatatctga aattggcaat cctattgatt attttctggg ctattttact ctcacagtta
     5461 aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg tatcttgttg
     5521 ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc tccactacaa
     5581 acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac gctggttgtt
     5641 tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca tgcaaatgaa
     5701 atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat tctggcaatc
     5761 attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca tggtttgtct
     5821 cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga tccaaatctt
     5881 gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg agaaattgct
     5941 tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg attacttcct
     6001 gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat cgcaaatggg
     6061 atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa taatctttca
     6121 tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt tatgtttttc
     6181 gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta tcaggttcca
     6241 ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc actttttgct
     6301 ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg gatttatgaa
     6361 acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat ctacgcatgc
     6421 cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc aaatggaagt
     6481 aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag gacaagtggc
     6541 ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg cgatttatga
     6601 tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg ccatcgaaag
     6661 catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag aaaaagtctt
     6721 tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga atatggaaat
     6781 caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg caacaacctc
     6841 gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat ttatgaaaga
     6901 gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg aatcaagaac
     6961 aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga tcgaaggaga
     7021 cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca ccgctacagg
     7081 catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga atggcaaaca
     7141 aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg ctggtttgta
     7201 tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg gtgtaggcgg
     7261 cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc cggcactaga
     7321 tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca aagaactaga
     7381 cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc aaacggctca
     7441 aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc agtttctttt
     7501 tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac gatttacaga
     7561 tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc tggaaaatta
     7621 catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg aattccaaga
     7681 aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga tccttcacga
     7741 tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa gagagaagca
     7801 taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac agtttctgta
     7861 cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat tcacgagtac
     7921 agaactggct gatcttgaat ttatttcttt gagtaaagta tatacaatca aaaaaatcgt
     7981 attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta tcccagaatt
     8041 tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag gctatgaaaa
     8101 taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag agactcattt
     8161 ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgagaaa tagagatcgc
     8221 aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa gcgcagaaac
     8281 gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa ttctagactt
     8341 acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt ataccaatga
     8401 gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg ttctcaatga
     8461 cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg acgatgcaga
     8521 aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg cagatggaca
     8581 agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc tgtacggaaa
     8641 aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa gatggaatga
     8701 aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac aagcggggag
     8761 acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt tttatcgaac
     8821 ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt tatttcgttc
     8881 attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt tgtgcgatgc
     8941 tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa tctcattaaa
     9001 aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa aaaaacatat
     9061 ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt ctgtagaagg
     9121 tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt tctctgaaat
     9181 ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat aaagtgtaac
     9241 aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat
     9301 tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa
     9361 tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg
     9421 ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg
     9481 attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt
     9541 taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca
     9601 accaacatga aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct
     9661 tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat
     9721 ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg
     9781 ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc
     9841 atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata
     9901 acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa
     9961 cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc
    10021 atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc
    10081 atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta
    10141 atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg
    10201 cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag
    10261 atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg
    10321 cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg
    10381 atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg
    10441 gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa
    10501 gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag
    10561 attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag
    10621 aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg
    10681 gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa
    10741 aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa
    10801 atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc acattagaag
    10861 atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa
    10921 tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc
    10981 tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga
    11041 atattcaata attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg
    11101 ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt
    11161 caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt
    11221 tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac
    11281 tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg
    11341 tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa
    11401 tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg
    11461 ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa
    11521 agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt
    11581 aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata
    11641 gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg gaggagatat
    11701 tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa
    11761 cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa aagaaggcgg
    11821 agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga
    11881 tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc
    11941 gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc
    12001 aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga
    12061 acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga
    12121 agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac
    12181 gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat
    12241 tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt
    12301 ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga
    12361 agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga
    12421 aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt
    12481 ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa
    12541 cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc
    12601 cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc
    12661 ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga
    12721 aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc
    12781 catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat
    12841 gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat catttcagta
    12901 ctagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata
    12961 cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta
    13021 aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta
    13081 agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc
    13141 gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa
    13201 gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt
    13261 ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa
    13321 atgccagatc aaacgattag cggattgttt aattac
//
LOCUS       DAJSVF010000070        13044 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSVF010000070 DAJSVF010000000
VERSION     DAJSVF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.073"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(241..855)
                     /locus_tag="ON550_002049"
     CDS             complement(241..855)
                     /locus_tag="ON550_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3955126.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1461..1838)
                     /locus_tag="ON550_002050"
     CDS             complement(1461..1838)
                     /locus_tag="ON550_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955127.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2138..3019)
                     /gene="galU"
                     /locus_tag="ON550_002051"
     CDS             complement(2138..3019)
                     /gene="galU"
                     /locus_tag="ON550_002051"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3955128.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3062..4081)
                     /locus_tag="ON550_002052"
     CDS             complement(3062..4081)
                     /locus_tag="ON550_002052"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3955129.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4714..5718)
                     /locus_tag="ON550_002053"
     CDS             complement(4714..5718)
                     /locus_tag="ON550_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3955130.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5731..6780)
                     /locus_tag="ON550_002054"
     CDS             complement(5731..6780)
                     /locus_tag="ON550_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3955131.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6797..7618)
                     /locus_tag="ON550_002055"
     CDS             complement(6797..7618)
                     /locus_tag="ON550_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3955132.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7605..8384)
                     /locus_tag="ON550_002056"
     CDS             complement(7605..8384)
                     /locus_tag="ON550_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3955133.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8401..8871)
                     /locus_tag="ON550_002057"
     CDS             complement(8401..8871)
                     /locus_tag="ON550_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3955134.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8883..9302)
                     /locus_tag="ON550_002058"
     CDS             complement(8883..9302)
                     /locus_tag="ON550_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR3955135.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9383..12160)
                     /locus_tag="ON550_002059"
     CDS             complement(9383..12160)
                     /locus_tag="ON550_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3955136.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12351..12575
                     /locus_tag="ON550_002060"
     CDS             12351..12575
                     /locus_tag="ON550_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955137.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3826 a         2730 c         2119 g         4369 t
ORIGIN      
        1 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
       61 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
      121 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      181 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      241 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      301 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      361 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      421 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      481 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      541 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      601 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      661 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      721 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      781 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      841 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      901 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
      961 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
     1021 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1081 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1141 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1201 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1261 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
     1321 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
     1381 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
     1441 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
     1501 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
     1561 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
     1621 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
     1681 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
     1741 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
     1801 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
     1861 taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc
     1921 ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt
     1981 tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta
     2041 gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca
     2101 aatagccttt cattctcagc aggccactta tttccagtta actttctgtt cttagctttt
     2161 tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat
     2221 attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa
     2281 actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta
     2341 tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta
     2401 gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa
     2461 acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt
     2521 tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat
     2581 aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca
     2641 tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc
     2701 ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag
     2761 cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa
     2821 agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg
     2881 aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt
     2941 cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga
     3001 ctgctttttt gaccttcact tgctgttcct cctaaacgac gattaactat tgatcatcta
     3061 tttaacgaag agaaaattca ttctccattt ttccgtcacg taacatgatt tcttttgctg
     3121 cttttttgat atcttctcca ttatacaaaa cattatagat tgtttgagta attggcatct
     3181 caacacctag ctgttgagct agctctactg ctgcttttgt cgttgccaca ccctcaacga
     3241 ccattcccat gttttctaga acttcttcca gcttatgtcc ctgtcctaac aagttaccgg
     3301 cacgccaatt gcgagaatga acacttgtac aagtaacgat caagtccccc acaccactca
     3361 aaccgataaa tgtcagagga ttggcaccca tcgcgacacc aagacggctg atttctgcca
     3421 atcctcgagt catgattgct gcttttgcat catctccgaa tcctaatcca tggatagcgc
     3481 cggcaccaat tgcaatgata tttttcagtg ctgcaccagt ttctacgcca atcacatcat
     3541 tatttgtata aatcctgaaa taatcattca tgaacagttt ttgtacatag gctgctgctt
     3601 tttgatcaat acttgcagct gtaattgttg tgatatcatg tactgccact tcctcagcat
     3661 ggctaggacc agataataca acaattgcct gtcttttttc ttctggaatt tcttcagcca
     3721 aaacttctga gattcgttta tgcgtatctt gttctagtcc tttacttgca tgtataatga
     3781 ttggcttagt attcatttta ccaataagtt cctgtgcgac tgaacgaatc gcttttgttg
     3841 gaacaacaaa taaaatagca tctgcgttat ccactgcttc tgccaattct ttatatccga
     3901 taatcgattc tgggatcgtc aaatcaggta aaaaatgctg atttgtatga tacttattga
     3961 tttcatcaat ctgttcaggt acatttcccc aaatacggac atcatgcccg ttttctgcca
     4021 atgtttgtgc taaagcagtg ccccaagatc caggaccaag aactgcgatt ttttgtttca
     4081 tggatattcc tccaaataaa taattgataa tttccattat agtatacatt tcttaaagtt
     4141 gggactaatt tccattgttt ttaagtgttt gtttacttct tttatcgttt ttacttgaaa
     4201 taatcaatga tatgttacaa tagtctacat tttattttta cactcgttat gctttgcttg
     4261 ttcctaagag gaagcagttt tgacaatgtt actaccagca ttactattag gaggttagga
     4321 aacttctcct agtgagtatt gatttaaaat acataagaga cagattgaac atttctaaat
     4381 ctgtcaggtc ctgcaacgga cagattgtct ctgttgacgg ttgacatagg aacttttgtt
     4441 ttagtcaaca ttctggatac aaaacgctat gtaaactggc gcaactcgtt gtacttggac
     4501 ttttactacg gatccatgca acaactaaat agtactattg cataacaaaa aatggttggc
     4561 acccacatgt cccattaaac agattataag ttgaactgta gataatgcaa aaaatgtgtt
     4621 ttataacgta attgatgcca atattttaaa aaaataaagg gagcagttat cgctgctctc
     4681 tttatttttt atattctttt tatccagtct atcctaataa tcaaactgac gatagtagcg
     4741 gcgtatctct aatggatggc agttcaactg ctccacatga gcatcgatgc gttgtgtcac
     4801 tacatgcatc aataagaaag acaaaacacg tccgcgatat ttttcactga taccttctac
     4861 tggatagtct tttgtgtcta tcaatgtata attgctgcaa atttttggca aaagtttagc
     4921 cacacgttca gataacactc tctgttcatc ttcgcctaaa aataatgtga ctggggttgt
     4981 ttcttctacg atctctaacg tcccgtgaag aaactcggca gcatggatag atttcgatgg
     5041 aagccagctt tgttcttccc agtaacacat ggcatatgaa taaacagctc cccattgatt
     5101 ccctgctcca ataaaataat gcatactatc acgtcgatgt ttttctgcaa atgataagcc
     5161 aaatgcatct gcttttttct ctgcttccac taaccctgtt ggtaaataac gttcgagctg
     5221 ttcatagtat tctgaataag cttcgaattc tccattcaaa tacatcaatc gatctgctgc
     5281 cataaaaaat ttgatttgtt ctgttccagg agctggataa gtgaccacaa aatcgcatag
     5341 ctgagacaac ttactgttcg ctttatcgat aaatccaatc agtgtcgctc ctactttctt
     5401 gatttgttct actgctttca ctacttcttg agttgtaccg gtaacagagg acaaaataac
     5461 gatagaatct tttgttaatc ttttgtttcc agtagtgtaa tattctgcag catgctgtac
     5521 aaagacaggc aagttgcttt tcccattcat ataagtgaca gcttgcatcg aggaagcata
     5581 cgttccaccg attcctagat aatagacagc gtcaaatttt tccgcataaa gtttatcgat
     5641 gatttcttct acttgcggac gtaaagctag tgcgcctttt attgctttga tttgttcttc
     5701 ttcattgaat ttcagcatga tttccacctc ttataattta cttttcaaga ctgtatcaaa
     5761 gtcttcaaaa atctttttgt tcaagtcaat acctttagct actgcggttt tataagcgaa
     5821 tagttgaaac ggaacgacaa gcgctaatac agacagatat tcatcacatt ctattgctaa
     5881 tccaagtgtt ttctcgtttc ttgctttttt tgtggtaatg gtgagagtct ctccaacgta
     5941 ccgactcata tatctttgca acttttgcga acgttcctta tttacacttt ccccttcaat
     6001 aaagaatagc aaatgcgaag catctgcctc caggtatggg ccgtgcatat aagcttctaa
     6061 ctcaaatcct tgtgacggaa cgcggattgt ttcagtcaat ttcgtctctg cttctttagc
     6121 tgttccccag tttggaccat atccaatggt gacgaaacgc tgtgctaaac gaaataaact
     6181 ttgatatcca tcaaaaaatt cttccgtttt ttgaataatc gctggtaaat ggttgattat
     6241 tttgcgcaac tgttgcatgt agtcttgttc tatttttttg ctgattttat ttttacttat
     6301 tccaattcct atccctaaga gtagtaactg taaaacagtc gcagaatagc cttttgtaac
     6361 gaagcctact gattctaccc cggcacctaa atcaataatt tgtgcagctt ctaaagcaag
     6421 tggactttgc acgtcatttg tgatagcaac aacaggtaac ccttgttttt gtatcatttt
     6481 tactgcttca acagtagaag cacttttccc gctttgtgac acagcaatca cagtatcgat
     6541 tgaaggagaa agttttccat aattgaaaaa ataaaaaggt tcttgaatat cgacagtaag
     6601 atcaccataa gattccaatg ctggcttcgc tgctaaacaa gcgttataag atgaacccgt
     6661 tgctaagatc agtaaattca ctgttttttt catatgttct cttgtcgaaa aatcattctt
     6721 tttcaagatc gtacttagta cttccttttc ttcatagata taatcttgca tcgtaaacat
     6781 tttctctgct ccttttttaa aaaatcccta tccacgaacc aaaaattcca aagaaagcta
     6841 gtccgatcaa gatcgttaat ggtttcactt ctttcttcaa tagataaaac actatgccaa
     6901 atgctccaag acataagata tttggaacga tatcatcaaa aatactttga acagtcacag
     6961 cattttctcc tgaaccgatt tttaaaggaa tattgatggt gatcatcgtg gcagtcattg
     7021 ctccaacgac catcagtcca ataatagacg cgcctaatgt caaactttcc atcataccgt
     7081 ttgcctggat ctttttcaaa aaacccgttc ctaagcgata tccccaacct gttgctagat
     7141 agcgaaacag aatgtgcgga atattgaaaa tcaaaatgaa caagataggg cctaaaatat
     7201 ttccttgcat ggctaaagag gtacccacac ctgtggcaat caatcgcaaa gttccccaaa
     7261 agaaagaatc tcctaaccct gcaagaggtc ccatcaaaga tgttttgata ctatcgatcg
     7321 cagtcacatc aaattcagga tcatccttat tttcttcctc cattgcagcc gtgattccta
     7381 aaatcagtgt aacgatatgt ggggtcgtgt taaaaaactc tagatgccgt ttcaaagctg
     7441 ctgccatttc ttctttcgac tgatataact ttttcaaaat cggaatcatt gcataaacat
     7501 aagccatatt catctgacgt tcataattcc aagaaaactc catttgaaaa gaacgccaaa
     7561 atacgtgatt tagctctttt ttcgttattt tgctgttttt gatctcagaa ttcttcatct
     7621 tcttctacct cccctgcagt tgtttgaagt ggtagttgac ggacattcat aatattgact
     7681 accacaactg ctaaaatcgc accaaaaata gctactccgg tcacaggaat ctctaagtaa
     7741 gaagctatag caaaacctaa gaaaaaatat ggtgcaactt gtttgttgat cagcaaacga
     7801 gcaagcatcg caaaaccaag agctggaatc aaccctgtag ctactgacaa ccccttttga
     7861 acgaagtcag gaatcatatt taatagattt ccgatcgtat tgcttcccac catataagaa
     7921 accattacca caacaccaag catcaaggaa agaccgatac cggcaagcaa gtgcatatgc
     7981 tcgactcctt tataatttcc ttcttctgca taattgtctg ctttatggtt cataattgga
     8041 atcaaaagcc ctaagtaaat atttttcaat atcaatgtca acgtagcaat cggcaaacct
     8101 agtaaaagcg cggtttctgt accggctcca gctgtaatag caaaagccgt ccctaaaatc
     8161 ccaccggtca caacatcagg tggaatcgaa gcacctactg agaacgatcc gataaatgct
     8221 aactctaaag ttgcgcccat aatgatccca gtttttacat cacctaacac gattcctgta
     8281 aacagccccg tcacaatcgg acgagaaagc aacgatgtcc ctagtgcata ttctgattgt
     8341 gcgacaaatg caacaattcc taataagact gcttgtacaa tcattgaggt tcttcctttc
     8401 ttacaaaact tctcttacat ccactttttt atctgctggt acttgacgga tttctacttc
     8461 tatttgtttg tcaattaatc gtttcaagcg ttcctcctct tctggtaaaa tatttaccgc
     8521 ttttccgatg ctgcgagtac caggttttgc cttgatccct cctaagttga tggttttgat
     8581 ttctggatac gcttctgcca gcttatatgc atcctcgatc gattccacta cgatgaataa
     8641 cttatattta tctgtcaccc cgcttttcaa cgcggcaatc gagtcatcaa ttgatttgat
     8701 cactagtttg actccttgtg gcttggctaa tttgatcgtt gtctttcgta tctcattcgt
     8761 aggtacatca tcattagcaa tcaaaataca gtctgcgcct attgtttgtg tccatgaaaa
     8821 agctacttgt ccgtgtaata accgatggtc gactcttgtc aatacaatca tttttcttcc
     8881 tcctaaaatt cgtcttcttc tattttctga ttgagccgct cgttacaaaa ctggatcatt
     8941 ttttttgaat cagttagcac ctgctggatc aaaacagttg tctctacttc agcgtccaac
     9001 cgagatacta gctcaatcaa tagtggcaaa ttcaatccgg caacgagata gacatgatcc
     9061 cgattgatat attgtaagaa ttcgttgttg acacttccgc caaaaagatc cgtcagaaca
     9121 atgatttctt tatctttgta cctcataaaa agctgcctta ctgtttccgc taaatcaaaa
     9181 ttctcatcag tatagcaatc gaatacagta atagaatgat ttttcccata gatcagctct
     9241 aatgaatcta atatccctga agcaaatttt ccatgggaag ctaagataat ttctctatcc
     9301 actttttcat ctcctctttt cacttacaca tactaaaaaa gcaagttgca tgccaaaatt
     9361 agtggcaaca acttgcttat gttcaaaatt cttcttctat cgagagaaac tctgtatctt
     9421 cactcaaaat atcatagata taggcaatct cagaattggg gatcatgaca ctataatgct
     9481 cttcaatgac actaaacgca gatttaatag cagataaccc ttctttttga cactgcttcc
     9541 atttctctaa tccttgatag gtttcgattg gcatttgtcg gatcaatcgt tcaatgagac
     9601 agctcacatg gacatacaac gctaattttt tgggattgct gatgcttcga ttcaactgca
     9661 cctctaattc tctcataaaa tgatcgattt ctcccatcac cttctctgta tctagaatag
     9721 tcacggaatc cagtactttt tccaatgaaa aattacgaat gatgcgctga ttgaatattt
     9781 ccttctctga agcattcata gtagtgtcca accattcagc tagtacagac atcttttccc
     9841 ccgagatgag gtcttctaat gaaagatagt cgatattggt gatatcagga tctgtggtgc
     9901 caatcattcc tacgacttcg tatatggaaa ataatgggtc actgtttttc ttatctttta
     9961 attgctgata ttcatatggg atgattttca actgacaagc cgtaggtaag cttttttcta
    10021 gcagttcact taagtggacc gccgtaccga ttccagtgaa gcaagtagta atgagtgctt
    10081 tgggtttgtt ttcctctgga tagagcagtc gccattctgg ctggatctgt cgaacagtct
    10141 cttctgctat ctcttctaat gatacatttt tttgcaggca ttcccctact gtgattgcta
    10201 aaggtgtcgt cacattattc aaaatcaaaa gcggtacgga taactgtttt ttaaaaaact
    10261 gatggatttc tttcagagat cccatgtcta ctaatatgat caatccattt gaaacatcgc
    10321 tgttttcgca ataatcaagg atttctgaag caatctgctg tggtgtgaca tctagtggca
    10381 tatcaaatga ttcaaataga tcctcttgta ataaacgatt tactacattt gccatactgc
    10441 tagcagttgc ataaccgtga gcagcaatca tcgcttttgg cagtcgtttt tcttttgacc
    10501 atttcgcttt ttttagatag atcgtcagta aaatccgatc catacttgtg tcttcaatat
    10561 ctaatttgat cttacttaat tccagaattc gttggacata gtgataacta gatggataag
    10621 actgagaaac ttgttgttct aattctttga ttagttggat aatatcatgt tcttccggta
    10681 accatttgat agtgcttcgc tgatagagat agtaactcaa ggcatagaca ctgtttccat
    10741 cgaatttgat ttgataggca gtttccattt gtcgaaaggt ctctcggata gattgtgtcc
    10801 aataaaccag cagctcgtgt ttttcttgtc tactggtctc aaacagcaaa aaatcaaata
    10861 attgaatcac ttcttgcttt aattttcctt cgcaagaata tagcagacag ttatttttct
    10921 tgaaatcaat taggatttgc tcaaaacttt taacgattcg ctgctgttcg ggctgttgct
    10981 tctccattaa ttgctctaag gtctggtctt gcgtaatata gattccttgc tgtgctgtat
    11041 ttttagtaat aggctgcgca agtagttctt tttgcaaatg atggatcgtg acatggattt
    11101 caggctgttc acgctgttca gcatatgctt ttgctgctga aactttgacg atatttttca
    11161 gctctcctat gtttccggat aatttctgac ttgttaaaag tgacaaagct ggtccactga
    11221 ctgacaaagg gctccccatt tttctttgtt cttcgagaaa aaaagagtaa atcagttcta
    11281 gccgttcatt acgactacgc tgattaagtg caggtagttc gattcggata ggtattcttc
    11341 gaatgaaagt tgttaagaaa tgactagtaa tctcttcagt cgtagcaaaa aagagacgtg
    11401 ttttgatccg gcgtgacaaa ttaggttctc ccatacgata aatcacacct tgatcaagat
    11461 aggtgaacag tttttcctga ccttctggtg ttaagcgatg tacctcgtct aaaaacaaga
    11521 tcccaccatc tgctgcttca aaagcccctt ttttattttc ttctgcacca gtgtacgctc
    11581 ccttaacatg tccaaacaaa ttgctagtca aaagttcagg attatctgca tattgtgcac
    11641 aattaaaagt gatgaatggt gcagaatctt ctagcagatc atgaagcaga caatactgat
    11701 agaccaaatt aactaaataa cttttacctg tgccactttc gcctgtaatc aaaagtggta
    11761 atccgccatc tggatagtaa agagctgttt tgatttgttc gatactttct ttcaaactgg
    11821 cgtcgtatcc gatcataaaa gagaataagt cttttggttg cgcgaaaaaa ggctgttcct
    11881 ctttgagatc atcgatagaa aaataaaaat tcgtgcgtaa cttaaaaaat tgtttttcaa
    11941 aagcttcttt gtggaaataa atcactggtc gagaattgat tttgactagt agttgctcat
    12001 catttaactg gttcaaataa tgactgactg tgtttctttt taccccaaaa tgatcagcta
    12061 acctttgtgc tgtaaagacc tcgttcatat gttctaaatc gaaaaaggct gtctggttct
    12121 ctaagtaaca taaaatttcc ttcttcaaca acttattcac ccctcctcca tcatagaaaa
    12181 cggattcatt ttgtcaataa aatctttaat aaagaattga aaaatatatc ttattataga
    12241 cagcagccta cttatccctt ataattttta ttctaaaaaa cgtacagttc cttccagttt
    12301 ttatactgga atttcaacat atgaaagaat aaggaggcta tgactattca atgaaatcgg
    12361 ataaatataa aaacaggaaa catacttcct ggaaaacttt tcgagaaatc atcggatctt
    12421 atctcgctgt agttgtattt ttgcctttgc tttatatggc tatgacagaa caaactcgtt
    12481 ttattggcgt agatacgcaa tatatagccg aaacaggatt tttattcatc tttatcttga
    12541 tttttgcgtt ctataaatcc ttccatacaa aataagattc acagatttca actttcctgt
    12601 ctgtttttag ctattacaaa tccttatatg tggtataaca acaatttatt ggcataatga
    12661 ggaaaaacga gttatgatgc tttcagagag taaagaggta ctgttgtcac catccttttt
    12721 attgattacg tacaccactc ttagtttttt cccattgatt acgaaaacaa ggtagaaaca
    12781 ttgatgttcc taccttgttt tcttttttta ttttcagatc aaaatctcaa gtagcttttg
    12841 acttgatgtc gggaggttgt cttcgttcca tcaaacctga tagaatctgc gccaaaccat
    12901 agttttctcc actattattt attcctctaa gatagtaccc acaataaacg aataaatatt
    12961 tttctcattt tcatactcct tctttgaaaa tcaattagat ttctctcaac ccactttttt
    13021 acaaaaatta cgtagcaaaa aaga
//
LOCUS       DAJSVF010000071        12982 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSVF010000071 DAJSVF010000000
VERSION     DAJSVF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.074"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(129..2219)
                     /locus_tag="ON550_002061"
     CDS             complement(129..2219)
                     /locus_tag="ON550_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3955138.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVSGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKDGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVECGSNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQDG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSTGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVAAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFSYAVLKFN"
     gene            complement(2216..2476)
                     /locus_tag="ON550_002062"
     CDS             complement(2216..2476)
                     /locus_tag="ON550_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955139.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(2489..3250)
                     /locus_tag="ON550_002063"
     CDS             complement(2489..3250)
                     /locus_tag="ON550_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955140.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTVADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLSTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQA
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(3266..4018)
                     /locus_tag="ON550_002064"
     CDS             complement(3266..4018)
                     /locus_tag="ON550_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3955141.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAAGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAASTDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVSFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(4033..6009)
                     /locus_tag="ON550_002065"
     CDS             complement(4033..6009)
                     /locus_tag="ON550_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3955142.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(6062..6736)
                     /locus_tag="ON550_002066"
     CDS             complement(6062..6736)
                     /locus_tag="ON550_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3955143.1"
                     /translation="MDEKMVIFFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKIPV
                     DQINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIF
                     KGTNQYTLSLGAATYFYEDGEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSN
                     DGVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYV
                     VQGELQ"
     gene            complement(6814..7976)
                     /locus_tag="ON550_002067"
                     /pseudo
     CDS             complement(6814..7976)
                     /locus_tag="ON550_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            complement(7982..8671)
                     /locus_tag="ON550_002068"
     CDS             complement(7982..8671)
                     /locus_tag="ON550_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCR3955144.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(8798..8992)
                     /locus_tag="ON550_002069"
     CDS             complement(8798..8992)
                     /locus_tag="ON550_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955145.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYISDMQITGKEKACLLGSMKNFE
                     QLRRTYVKIRSNCQLLLEVS"
     gene            complement(9102..9341)
                     /locus_tag="ON550_002070"
     CDS             complement(9102..9341)
                     /locus_tag="ON550_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955146.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            complement(9508..9849)
                     /locus_tag="ON550_002071"
                     /pseudo
     CDS             complement(9508..9849)
                     /locus_tag="ON550_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
     gene            complement(10468..10800)
                     /locus_tag="ON550_002072"
     CDS             complement(10468..10800)
                     /locus_tag="ON550_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3955147.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(11621..12661)
                     /locus_tag="ON550_002073"
     CDS             complement(11621..12661)
                     /locus_tag="ON550_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3955148.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT         3790 a         2543 c         1955 g         4694 t
ORIGIN      
        1 atcgttggtt tatcccatgc ttatgaagtt tctgcagacg gatcactttc ttcagacaac
       61 gtatttatta gtgccaatcc aattttcaaa tagaaaaaca aggctgattt tcagccttgt
      121 ttttttgttt aattgaactt cagaactgcg tagctaaaag ttgctaacac aagtaatagt
      181 aagctgaccg ctaaaatcgt tactaaatgt gtagaacctg ttttaggaac aataacttgt
      241 ggcaagttag gatcaacccc aattgtcact acattcgttt tattagaaac attatctact
      301 gccgctacag cttggttatc aatatttttt tctgattgta atggagtttt agctgtaaat
      361 atcagctgaa tttctcgtcc atctaaaaga ttgttactta agaagtcagc ggaagcttcc
      421 cacttcagcg ttcttgaatt gctatcatag gttagtgtgc cttcagaagt aatatctttt
      481 cctgtactct tatctattac ttgcgcagaa ccttctttat acgtcaaacg agcttcaatc
      541 ggatcttgaa tactaaattg cttgtagtga tctgacgcag cgtctacgcc ttttgtatgc
      601 catttttgtg tgactagata ttgtacttca gaccctgcat ttaaaggagc tgtcggtgta
      661 cccttcttac cgtcatttcg tccgtttaca tcttttccat cttgttgcac ttctttcact
      721 ggaattttag ggaaataaac ggtaatgata tttgtatcct tttctacttt attaatgatc
      781 gaagttccct gatttttggc tacactattt tgattggcca attcattgac ttttacattg
      841 atcactaatt cgtaagtttc gccgttatac ttcattcgat tttttaatgt atcagctttc
      901 gctgcatatt ttacctggtg ttttgttttg tcataagtca cttcaccaga tacatcgaat
      961 tttttatttg atccacattc tacgtgtgca tcgacgtaat ctacttcttt aggtaaatta
     1021 tcaattaact caaatcgatc gtaaaccgca agtaaatcca ctcctaaacg attcactttt
     1081 tgtttgattc gatattgaat tgtatctcct acttgcaaca ttttatcgtt cacgttattt
     1141 ccatctttat ctacccctgt tttttcaggt tgatcaggaa caacgctaaa gattgtcgca
     1201 gaagataaag taaaccaggt ttgaacatta gaagaaccaa tcacaaacgt cggatttgct
     1261 tcagatattt taaaggacac cgtcccttga ccaaacttcg gatctcctaa tttatcatgc
     1321 aaattgttga aatcattatc cgcttgtgtc agcttagagc ttcctcctgg gtgaccacct
     1381 actacgttca gattgttgta gaaagattta aattctgaaa gaaccgtatc ttttctgaca
     1441 taccaatctg tcgtattcat tttgtcatag tgggcatatt ctcctttaag atccgtgctt
     1501 tttcctcctg cagggttcaa agagttaaaa ctgataaatg tatcccctga aaattgaacg
     1561 ggactaccgt cctcatatgt ggctactagc tgcacattca tttgtgttga atggtaaaca
     1621 attcctgaga aaagattgtc tgagatccaa aggactgttc tttttttaga tttactcatt
     1681 gcaccattcg tatttttata gccatcgtct cgaaaatgag tttttgtata gttattatct
     1741 agtagattcc acggcaacgt atctgaatat aagtggatat tagatagaac tagcttgata
     1801 ttcacttttt tcccgttata cgtaccgatt tttccgtagt caatattgac tttggtgttg
     1861 gttgttacgc cttctaaaac aaggtttgtt tcaccatatt ctttttggaa agaagggtaa
     1921 cgtatatttt tgtttccctc cactaatcct acgtagtcgt ctgtaaaacc acctttgttg
     1981 gcatcaacat gatatttgta aactttggac gctccgctta cagtaatatt tgtttttgca
     2041 ttaggtacat cttttggaat aataaaatta ttctttacgt tctggattgg atttgacgga
     2101 tcgacaataa ctttttcagc aaagacattg gctgttttta acccaaacaa tgtacctaac
     2161 atcatcatta tgggaaaaac atattttaac cgtcttgtcc acgagtgttt aaaattcatc
     2221 ccttcatccc ctctttttta taaatgacta gtgaaattcc gacaataacg atcaaattga
     2281 tcgtagtaaa taatggattc ctaatgagct tttccataat atctagcggc atggaagcag
     2341 ctggtatgtt taaaatcctc attaggtata gttggtagct tggcaaaata gatagttgag
     2401 aaaataataa accgcagtac aaaaagactg cggttaaaca agaaagattg aataataata
     2461 atttaatcag tttcatctta tttcttcctt aatctctaga aaacaagtga gtaacaaaat
     2521 cccaacaatt ctcaaagaag tttttcttta cgccagtaga aacgattttt tttacttctt
     2581 ctttaggttg cgaagcttct gatttaggat agatccacac atcctttaag aattcaccgt
     2641 ttttatcaga aacaggtgtt aatagcaaaa tatcctcgct attagaaatg ttttcaggac
     2701 ttgattcatt gacaatataa taagcttgtt tctgattagc ctgtacttct accgagaatt
     2761 caaagacacc tgcttgttga tcaattgtct ttgtttctcc attagaaact agcttcaatt
     2821 gatcactgga tagttttttc actcgatctt ttaattggga ttcaatttga tcagttgaga
     2881 gatccttaac atccatcttt tgtaaaatgt ccgatacatc ataaacagaa aacttcgcgc
     2941 catttactcc ttcttgtttt tcaagctttt gcccctggtt gtctaattgt tctgaagaaa
     3001 taatcttttg aacgtgaatg gtctgtttga ctatttgatc gtcttcagca attttaggtt
     3061 cttctgaaga atcaactgtt gacgaatcga cggtttcatc gctgctttga atcgtgctgc
     3121 tatctgacgt ttggattgtt tgttctgtgc tatccgtgct ttcttgactg tctgcaaccg
     3181 tcaactgtgt gtaggcagtg aaagaaaaaa gagcggctgc acaaacaaat agccattttc
     3241 tatttctcat gttggttacc ctcctttatt ttttcttttt ataaacaaat agcgaaccaa
     3301 taatcatgac tgaaagaaaa acagtacttc ctaaaagcca tttccaagat aatttttgtt
     3361 ttttattttc ttcatctttt aattctgatt ttgtaaagga tactcgtttc ccatacacta
     3421 ggaaacggtg tgtgttgatt cccttaggcg tacatgtcat tagcgttact cgatcttcat
     3481 tgggatttgg ttctaagtaa gtgacaaagt ttctttgtag ttcatctttt tgcacttcct
     3541 caatccgatc gatttgataa gcgtgctgct caccatcaac tttgatataa aacttttctc
     3601 ctttttttac tgaaggtaaa tcatcaaaaa gattatcttt ataaagtcca ctatgacctg
     3661 cgattaaagg atttttaccg tttcctcctg taatgtcacc agttccttcc gtaataccta
     3721 caccattttc taaaattttg tcggacgttc cgtcataaat tggtagtttt aaagaaatac
     3781 ttggaattgt aagaatacca acgggatcgc ctaactcaat cttagcaaca tccaaagaag
     3841 aatccgtgct tgctgccgtt cctgttcgat cgattgcggc aatctgctga ttttgctggt
     3901 atttcgccac gctcgcttca gcaggcatca aaagtaagac acctgcagct gaaagtaacc
     3961 agaaagcaat gatccattta cccatgattc gctttttctt aggcttcctg ataatcattg
     4021 gttttgatcc ttctaagctt tcttcttaaa gtaaacaact gcagaaagtg cccctgctcc
     4081 tagcaacaag caagagatca atacatacgt ttggaaaccg ttactaccag tcataggtac
     4141 ggaagctttt tgattgtttt tgatcgtgat tcggttttct tctttgtatg aatctttgtc
     4201 tactttgaaa gagatctctt tcgttaattt ttgatagttt tcaggcgctt ttgtttcttc
     4261 cagtgaatat tcaccttcag ttaaaccagt gatttcaaag tgaccttctt tatctgattt
     4321 taggatcgct gcatttgcta catcgtctac ccatactgct tctttgacac cgtctttatc
     4381 cgtatcaact aatccaccat aatattttcc ttctgcgttt ttagcagcaa attctgcgcc
     4441 ttcaagcggt gttgctttga ttcctgttcc aaatagtccg ctatcgactt ttaagaattt
     4501 tgcaccataa gtcacaacgt cagctttcgt tttttcgtgg tgttcttcct gattaaatgg
     4561 attgttgtta taaacgaaat ccacgctatt tgttaatgga ttggcaacag ctgcgctgtc
     4621 atttacatac atgttataag tcatttgaag atcttgtccg tgtaaacttg ctacagtatc
     4681 acttacttgg ccattattca cgataaaatc gatcacaaag ccattttctg aattttggat
     4741 cttataatct gtattttctt ttaataccac agatccatct ttatttgtta ctttgatatt
     4801 tgctttagca gcgacataag aaagtccttg tcctgctgta tccccccatt taaatacact
     4861 gtaaggagaa gtttcgcttt tcactccgtt tactgtgata ccgtctgctc cgcctgcaat
     4921 gtctgttggg atatgaatcg ttccttgata atttactgcg tcaccaattt ggaaagaatg
     4981 ttcttgtcct gttccatttg ttactgtttt atcgatcact ggtgctttat agtttacgta
     5041 tgaagctttt aagtcacttg tcacgccatc attcgtaatt tcaaaggtta atttattgtg
     5101 cgcatcgttg cgagcgtctg cgcctaataa atattgatct gctgttggtt ctttatctga
     5161 tcccactaca acttctgaag gaacttccac aaaatagtat tttcctaacg tcaaatcagt
     5221 agcagtcagc tttccttgat catccgatac aagatctcct aattttgttc cttttccagg
     5281 ttcgcccttg tataactcaa attttgcccc ttttaaaggt gtttgtttgc tttgagctgt
     5341 gccatcgtca ccaaatttcg ttaattcaaa actcaactta gaaacaatat ttttaggata
     5401 taagtgaatg tcttttaaaa aacctgaacc tgtattatca gtcattggcg caatcaccac
     5461 cattggtttt gctttttgtg tgactaatcc agcagcggat ttactttcat aaaccacata
     5521 tacattccct ttggaattga cataggctgg ttgatcagca aaagtgatct caccattttc
     5581 atctacagct gaagtaattt tgttggttgc attcttgacg tattcactgt tagatccctt
     5641 gtcttctaca tctttaacga tttcgtccac tttcgcgttg gttagatcag aatcttcagt
     5701 cggtaatact tgatccgtaa tatttcctaa cgtaaactca acatcaccat aatctgcctt
     5761 gttgtatttc gttacacctg taggatattg atcgtgcgta taaccatcgt ttttgattcc
     5821 gtcaatatca acattcaatt gcgttccttg atcgtacatt aatttatgaa ccgtgatatt
     5881 gaccttctct ggtggcgttt tttgcgtgac atcttctgca tgtgcttgat acccctgaaa
     5941 gcagcttgca atgattccta acattccaac aagcattcca gcttttttta cataccgatt
     6001 tattgccatt tttttatctc ctcatttcat atttcaacga ctcgcgttga ttaaaacctt
     6061 gttattgtag ctctccctgt accacgtatc tttcttttcc ttgtgacgta taaacacaag
     6121 taaacaacgt taataagggt ttctctcctt tctttggcaa agacaagaaa gagttctctt
     6181 caggaacgcc atcgattaat ttaaagtact caggtacgat aaatttacgc gttaccttgt
     6241 aacgatagac gccatcgtta ctaactaagt ccatcacctc ccctaatgac aacttttgaa
     6301 tatcagaaaa taaaacacct ggcatttcca tgttatgacc tgctaggaca tagtttcctt
     6361 tgcccatttc gccatcttca taaaaataag tagccgctcc aagactcaaa gtgtattgat
     6421 ttgtcccttt aaaaattggc aatgctaaac caagtgcagg aatctgaatt tgtgcaaata
     6481 cttgtttatt gacgcccatt tcgattgctt ctttttgtat ggacaacatt tgacccaaag
     6541 acatatcatt attgacgttc gttgcatcct ccttataact ctttgcgtgt gcattgattt
     6601 gatctacggg tatctttttt tctatatttt gcttaaacac ctctccttca tgtttttggt
     6661 agctaacata gccaaacaca ctgcaaaaga cgattaaagc gactcctaaa aaaaaaataa
     6721 ccattttttc atccatctgc ctcctttcag ataaaacaaa aaacactttt tcacaacgaa
     6781 aaagtgtttt ctcttattac tattctattt catttatctg gtggttttat attggtgtgt
     6841 agccattgct aacattaaga atacaattgg cacccaactg attgtagtta atgtttcttc
     6901 catgaatgat tgacacatga tcgctaaaaa agcccacaag aatgttagtt gtccgtttgt
     6961 cagctcacat tttcttacga gcaaccataa aaaactaagc aaaggaataa tcgatagagc
     7021 aaaaccaatc aatccatagt gattgacgag tgcgtgatac gtattatggt tacctagaat
     7081 ctgatttcct atatggaaaa agaaaggttt catccctaca aatatttgtc ctgttttttc
     7141 gcctaatgtt tcatagaatt tgcgccaaat ttcttctcgt cctgtaaata aatgaacttt
     7201 gtctgacatt gcagcaaaat aagaaatcgg taaagctaga accgaaactg ctgcatacat
     7261 ccaaaaggac aagtgtgtgt ttcgaaaaat cttcttaggt aaaatttcta ggataacgaa
     7321 gataacaagc gcgtataatg ctgctttcga ttggcaaacc caaatcgttg ccccagcaag
     7381 aatcaacgct ggtaatgata gcaatcgcat gtaccatttt acaaatgaac tagcaaatcc
     7441 tgtgataaga agggctaatg tcattaaaga actacctatc gtatttgtat tgatccaaat
     7501 atctgataat ttgttcgatg gattccaaat actttgtcct tctggtactg gtatctcggt
     7561 aaatactcga taaaccgtta cgagtaaaaa gacatagaat actacaagat acaagctacg
     7621 atcaaacaca gtaaattccg tccattttac aatcaaaata atggcaataa ttgtcggaac
     7681 gatcagatca ccatagaaag tatctactcg gtagcttcta aaaaaagaaa agaaaaggta
     7741 aaaaagaatg cctgctgcaa tgaagaataa atcgtttctc tttaccttgt acagattcaa
     7801 aagaaacaaa cagcctacga aaaacgtcac taactgtgca gcattatcat aaataatatc
     7861 gtaggaggat ttaaccccac cgtccatatt aaatatagaa tggataccaa cagtaacaga
     7921 aaaatggcac tcattttatt gatcaatttt tctacaagtt cttgaatgtg actcacttcc
     7981 cttatttatt gattttttct acattgacta attcgcactg gatcacatcg cactttttca
     8041 tattatttgc ttcaaagtct aacagtgtga gtagtggttt tttacctgct tttggcaagg
     8101 ataaaaagga attgttttcc ttagattgaa ctacctggct gtaatcataa atttttcctc
     8161 ttttggagac ttcatactga tacttgtaat gagcatcgat tagatcaatt tcttctcctt
     8221 cagataaatc aatcatatcg gataataaaa cgcttggtgt atctacgtta taaccagcaa
     8281 gaatataatt tccatttccc atttcagcat ctggataata agtagaagct actcctaaac
     8341 taagcgtatc gctattcgct ccttgaaaaa cagggagaat aaatcctgta gaagaaaaaa
     8401 caagttttcc agcaatttca cctttgagat ccttttctaa tgcctgtttt tgattagtaa
     8461 taagttgtct cttagaaata tccgttaaac tggttaaatt atcttgatag gttttatcct
     8521 gttcctgaat atggattgtt tttgttacac catctacttt ttcagcgatt ttgtcctttt
     8581 gatgaccttg ataagcaaaa taactaaaaa ggagaaaaac aatgattacg cccacgtaag
     8641 gattaaataa agttttattg tttttattca tctatctacc ttctatattt gaattaattt
     8701 acctacatct aaccaagata aaccgcgtta aattttacta ttctttttat taaataacaa
     8761 tgccacatca taattgatca ttttcatatc ataatcgtca agaaacttct aataatagtt
     8821 ggcaattaga tcttattttc acataagttc gtctcaactg ttcaaaattt ttcattgatc
     8881 ccaatagaca ggctttctcc tttcctgtta tctgcatgtc actgatatat tgcatgacag
     8941 ataacctttt aaggtgtttt tgtttcatcg tttttagtaa cttttcttcc atttctttcg
     9001 acactttttt ctattttcga tttctttaca attcatgagt tttcctacct tccttgtttt
     9061 gataaaaata aaaaagccta tgatcacata cgctctacac ttcactcttg ggttatcttt
     9121 ttctttcttt taactactgg ccttgctggt ttcaaatcct tgattccatt atagattctc
     9181 caataatcaa actcttttcg taatctttct ttttccatat ctgttccttt ggataaggct
     9241 ttaaatacat ctttaggtga ataactacga gctaacagct gtacatacct tccttttgtc
     9301 aaagtatcca tatccatcgc atctaaaact ggtcgcgcca tctaatttgc ctcctaaata
     9361 aattctttac tcgatgtcta atgataatac tagcttttta ttattcgtcg taatttctgc
     9421 acattcttct tcattatttt ctgcgattaa cgcataatag catggtgatt ttacttcgaa
     9481 aaatttcatg atctattcct ccaattttta ttcttcttta tccgctacaa agtcaatgat
     9541 ttcaacactg acaatataat cactcaaata tgaccaggta tcttctacag acaattcgtc
     9601 tcctttgtcg ttcgtaagcg tcattttaaa ccactcatcg ccttcatcat cttcaggtag
     9661 caagccgaat ccaattggat tatcagtcaa tgtttaatcg tataaatcat cctcgtctaa
     9721 tgctaaaaca tctaatcctt tacaatttcc gcctcttcta aacgtcacaa atcctttgta
     9781 atcccaacgt tggaaagaaa ccttaatagt atgtgtgccc aatcgtatat tcggatcata
     9841 atttttcatt tgtcattcct ccattacgta aatttcattt tctctatgtt ttatatcaat
     9901 tttttcactt tattcaggac ttgttcatat tttatcgttt ttcgtttact taaaaataca
     9961 taaaaaattt cgactcgttt atggcgtccg aaacgagaaa ccctagtaga cagcggtaaa
    10021 tgctcgattg atccaataat caacgtcctc gcaagtatct acatgtactg cagcacacga
    10081 cacaatttct tgtgcgtcct ccccattacg agaagaccta ctccttattc catgagtttg
    10141 cattaaagtc tagatccctt agtagctgct catgattaat ctagacgcta ttctaacttg
    10201 tctaacttct aaattccctc ttgtttactc tgcgcttatt atttacacaa attcatttca
    10261 ccactatcca aacaagcgca tttactcgat tgaccaattt tttacgttac caatcgttaa
    10321 aacctacccc gtcactcccc gaagggaatt gttgatagat caagtctact ccttattttt
    10381 ctagtgactc taagttcctt tagaagtctc gacaatattt tcaagcaagc gaaaaacgat
    10441 aaaatacaag ttatgagaat gatcaattca gctgatcaaa cgccccatcc cttttgcggt
    10501 acaacgcaag actccatttt ttcttcgacc aatcagtaca ccgttttggt cataaacatg
    10561 tttgattgtt ggatatagct cttttaaggg gataggatct tttaaaatta tttgcttttg
    10621 tttcccaatg taataaatca tttcctctga gactttgtct ttctggatta tatatttctt
    10681 acactgcaca acggttttac tttggccagt tgatagtttc ttcgtgattt tctctgtagc
    10741 ggtacaccgt ttaccgtagt gacaaatatc tttgagactc aaaattcctg tatatgacat
    10801 aaaataaacc acccaatttc tttaaatatt tttaacaagt ttttcttgtt atttttcaaa
    10861 aatagagaat tgattttcgt tcatgacaaa caaaaaagag ggaacgcatc caaacatgcg
    10921 ttccctctaa tttcagcgtt cgtcatacgg caaagagcca cacggacatt tttataacat
    10981 tatgcttagg catagattca tcgtaatatc acgtattttt aaccttaaca cagccaaaaa
    11041 aagtggtatc acttttgaat atctgttata ctaaatccgt tgcgggtaat accgtaacac
    11101 tgaaaggtta acgcattaag ggccaaatct taatgccgtg tatgaacgct gacgaagatt
    11161 accagccttc cgagttcata ccgcctttct tttttttgta ttttcaattg tccaaattta
    11221 tcgtccaaaa gtgcagaacg aaaatcaatt catcacaggc atagcagaca attaaattta
    11281 tacaaaaaaa tgtaagcgaa taatattcgt attgcctgaa aagaagtaat atcaagattt
    11341 ttagcttgtt taaataatga aattatctct cttcgcgtat tattataact caaaaaaaag
    11401 gagtatgcaa acatacgttc ccttttctat atttgaaatt aattatttct tttaacactt
    11461 atgacgtaaa aggctacgtt tgtgattttt atcataatat tttaacttat aaattctatt
    11521 aaaatagcta ttattttgta atataatata attagaataa taaaaaattt ttaattttaa
    11581 tgttactcca tgaagcaaca atcactttgt cagtaaagtt tcactctaac cagtctttcg
    11641 taatagtgct tggggagctt tcataattca aacgtttttc acttgatact ttagcagcta
    11701 ttggtttttc gataaaccaa ttttttacat agacaaaaat caggttctgg atattttcga
    11761 ctttttcggt ttttaatttt tgataggctt ttaacagtgt cgtgtataat tcttgttctg
    11821 cattgacata ataactatcg agttcttcaa aaactatcga aataccactt tctatttcag
    11881 cttttttctt tgcattatga atagtctgga cggttttttg agcttcactg taacttgaag
    11941 aaaatgtttt tatcaaattt aatactcgtt cagggacaaa cgtagcaatg ctgtcgtttg
    12001 tcataattgt ctcaaaattg tctaacaaca attgatcttg taggcggtct ttttcagtgt
    12061 ctatattgtg tctattagtg tctaatatat tattattaag ttctggtatg attttcatac
    12121 cctctgattt atcaacgttt tggcacggta tgattttcat accctcttga gccccaaggg
    12181 attcagcgtc tgatttttcg tcgagggtgg gtatgatttt cataccctct gatttatcaa
    12241 cgttttccct attctctcta tcaaattttt cgagtaagta gatatcattt tctgagactt
    12301 ttaattctgc tagatatagt ctattaggat tatttttccc taactgtttg ttaaatccca
    12361 tctgcttttg taataataaa ccaaaggatt ccaactcttt tttgattgct atcgcttttt
    12421 gtttttggca gtttaatacg ctacatagtt catttgtcgt ataagtaaaa taaacattac
    12481 cttcttcatc gatttgccgt ttgctgattg cgatctctaa tcttgctttt aaaagcatat
    12541 aggcaatctt agttgaatct ttcatttttt tgtattcgtc agaaacaata aagacttttg
    12601 gaaattgata aaataattcg ttataaactc tatccgcctt aaaaaaatta aaatcagtca
    12661 ttgtagatcc tctactttca cgttgtttta tttatgtaaa attacctact cttttcgaaa
    12721 atagcaaaga taaataaaaa atgcaaacgc attaaaactg ttttttaatt aaatactacc
    12781 taaaatcaga aataaaatga attatgtact acctttaaag tcattaagaa ctactttaaa
    12841 taaatattat ttataattaa atactaccag aagaacgtat gaataaaatt tagttttaaa
    12901 attaaagata attaagtact actagtgtca aaacaaaaaa taaattaaga actaccttat
    12961 gcatataaaa catactacca tt
//
LOCUS       DAJSVF010000072        12434 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSVF010000072 DAJSVF010000000
VERSION     DAJSVF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.075"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            560..1531
                     /locus_tag="ON550_002074"
     CDS             560..1531
                     /locus_tag="ON550_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3955149.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1609..1845)
                     /locus_tag="ON550_002075"
     CDS             complement(1609..1845)
                     /locus_tag="ON550_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3955150.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1814..1945)
                     /locus_tag="ON550_002076"
     CDS             complement(1814..1945)
                     /locus_tag="ON550_002076"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3955151.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2021..2404)
                     /locus_tag="ON550_002077"
     CDS             complement(2021..2404)
                     /locus_tag="ON550_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3955152.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2437..3222)
                     /locus_tag="ON550_002078"
     CDS             complement(2437..3222)
                     /locus_tag="ON550_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCR3955153.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3400..4500
                     /locus_tag="ON550_002079"
     CDS             3400..4500
                     /locus_tag="ON550_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3955154.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4490..5314
                     /locus_tag="ON550_002080"
     CDS             4490..5314
                     /locus_tag="ON550_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCR3955155.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5311..6126
                     /locus_tag="ON550_002081"
     CDS             5311..6126
                     /locus_tag="ON550_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3955156.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6123..6953
                     /locus_tag="ON550_002082"
     CDS             6123..6953
                     /locus_tag="ON550_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3955157.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6960..7991
                     /locus_tag="ON550_002083"
     CDS             6960..7991
                     /locus_tag="ON550_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955158.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8098..9371)
                     /locus_tag="ON550_002084"
                     /pseudo
     CDS             complement(8098..9371)
                     /locus_tag="ON550_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9648..9857
                     /locus_tag="ON550_002085"
     CDS             9648..9857
                     /locus_tag="ON550_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955159.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9907..10623
                     /locus_tag="ON550_002086"
     CDS             9907..10623
                     /locus_tag="ON550_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCR3955160.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10531..11464)
                     /locus_tag="ON550_002087"
                     /pseudo
     CDS             complement(10531..11464)
                     /locus_tag="ON550_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            11752..12405
                     /locus_tag="ON550_002088"
     CDS             11752..12405
                     /locus_tag="ON550_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3955161.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3600 a         2292 c         2831 g         3711 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaaaaga ggactacctg
      421 tattagtcct cttttttgac cagattttga ccagtctatt ttacatccta gtatatttat
      481 atgagctaat tttatgatta aatggtaagt ttttccttgt gtgttttagt ttaaactagt
      541 gtgtaattca aaaatctata tggaaggaac agtattaaga atagaacagg gttctctaca
      601 tgatggtgct ggtttacgga ccgttgttta tttaaagggc tgcccattac gctgtgcctg
      661 gtgttcgatt cccgaatcac aaagcaagca gattgaaaaa ggatttggcc agaccatgac
      721 cgctgaagag gtaatggatg agattgagaa ggatgctgtt ttttactttc actcggatgg
      781 cggagtgacc attagcggag gcgaggcact ggttcaggcg gattttgcta aagaaatttt
      841 gcaaaaatcg aaatacattg gaatcaatac cgtactggaa accagctttt gtggtgctta
      901 taatgaaatc caaaaggtag ctccctatgt ggatacattg tttgtggatg taaaaatgtt
      961 taccagcaag ctccacaaac aatggaccgg cttagataac caacaaattt tgaagaatat
     1021 tcgtcgcttt ttaattgagt atcctaactg cgaagtgcga attcgagttc ctgtggttcc
     1081 aggtatcaat atgaacctga ccgagctgct gacgattgct tgttttgtgg cagatcttga
     1141 tcggttcgta ccgttagaat tactaccgta ccactgctat ggtatgcacg gttaccaagc
     1201 attgggactg gagtatccgt tggcagacac gccagcgcct tcacctaaag aaatgtttgc
     1261 attggcagat caactggctc gcacggttcc tcatttgctg gtcacagctt taaccaaaac
     1321 atttatttat ttattgaatc caagctttat cttatggata aatgaaagac actcactagc
     1381 tgcagccagc tcaagaatag agcttttgct gaagtcagtg agtgtctttt tacgatgttt
     1441 tttcgaagtt aatttgtcga atgaacactg gctcaatcgg tacatcttca ctgtttgtcg
     1501 gctgagctgc gatcgctgct acgatttcta acccttggta tacctgtcca aaaacagtat
     1561 aacgaccatc cagctccggt agtcctccaa gctccaagta tgcttgcttt acattagcag
     1621 gtcagcctgc gcctttgtgt tttgcacaat aaaaaactgg ttgccgttgg tgtgggggcc
     1681 gtggtcccct aaggccaagg cgccattaaa atgataaagt ccgcgatgca cttcatcagc
     1741 gaaaaagccc tcatagctgg aaacagcttc atcaccagaa ccatccaaag cgccaccttg
     1801 aataacaaat tccttaatga cacgagccga aaggtgtgtt gtcataaacg ccttgctcag
     1861 ctagggagcg ccagttggct acagccaggg gagcttggtc aggaaataag cggagacgaa
     1921 tttcaccagc atcggtgacc agcacacaaa cttcttcctg ctgcggtttt tctaattgaa
     1981 agagaggaag gtttgcaact gaaacgcctt gatcctgcca tcaaatatcg aaaatccgcc
     2041 actcgacctt cgcctgatca ccaaggcgag ccattcccaa taaaacggta taggcggttt
     2101 catccgcttg aaaggtcaat tgcagctgtc ctcgtaaatc gttgtagtca tctggggcgg
     2161 ctacttgata aaaaagatga aactgcgcat gcaaatattc caaaggctga tctttaaagg
     2221 ctgcaaaata agcctgaact ttttcccgct gcaagctgtc agccaccaaa aaattttcca
     2281 gttgttgttt tggaacggct gctaacaagg atcggcaaac cgcctcaatt ttcaacgcat
     2341 cccaatgtcg cgcaattaaa taatggatgg gtgcctcttg attgtagtcc cacaggttgc
     2401 tcattttttt caccactttc ttctattata taggaatcac aaaatcggca gcggcgtcag
     2461 gcggcgttct tttccttgaa tgactagcaa cttttctacc tgacagtctg ccaacagctg
     2521 actcattttt ttaaagccca gctgataatc ttcggcaaca tgagcatccg agcctaaggt
     2581 aaagagcttt ccgccgacac tttgatacaa aggaacggca taacggtaaa ggtcttcgtt
     2641 gccgtatttc aagaagctct tggcatttaa ctccatcgcc aattctcgtt caacaacctt
     2701 tttgaaaatc gttagcagct gagcctcaaa atgctccgcg aattcttccg ctgtgaaggt
     2761 gaagcggcgt aatccataat caaagtgagt caaaatatct ccttctggaa aatccgtcaa
     2821 gacctgcaac atctgatcaa aatattcctt ggtagtttga atgggatctt tggttaaaac
     2881 cacatcgtcc atataatcaa agcggccatt ttgatgaata cttaccagct ttaaatcata
     2941 aggatgctga gcgaggtaat ccacgatccg ctcttcttgt cctggaacaa cgccaatctc
     3001 gatacctcgc agaaatttgg tggaataggt ttcttttaaa gagtccagtt tgtttatata
     3061 ggctggataa tccggaatgt catcttttcc ggtagatgga ttgtttaaat caaagtgatc
     3121 cgtagaaacg aaaaactccg gctgataggc taaatagttt tcaaattttt cctctgaatc
     3181 aaaggaaaaa tacgtgtgga gatgctgatc ataatactgc atagggagcc tccttttttc
     3241 tactatcata ccgcaaaagc tttagaaggt taacgggaat tcgtcgaaat ttacaaacca
     3301 tatacaacct ttacattgtt catgaaacct tttcaagaaa ctgattctct cattacaaag
     3361 ctttgttaga atggaattat cgaaagggga gaagaatgaa tgaaaatgaa tgaaaaaatt
     3421 cgtaacatga ctatcggttt attttgtgca gcaacagtaa gtatcctgct gacaggctgc
     3481 ggctcaggtg aagcaacgaa gcaatcggct gcggcagacg actctttaga tacgattata
     3541 gaaaaagcca aggaagaagg agagattgcc agcttgggaa tgccggatac ttgggcaaat
     3601 tgggtaggca cttggaacga ccttgaaaaa gaatacggct taagtcacgt ggatacggat
     3661 atgtccagtg cagaagaatt ggctaagttt gaatcagaag gaaaaaatgg aaccgccgat
     3721 attggtgatg tcggtatcag cttcggtccg ttggctgaaa gcaaagagtt aaccttacct
     3781 tataagacca actattggga ggaaattccg gattgggcaa aggatgatga tggggattgg
     3841 ttgttaagct atacgggaac catttccttt atcaccgata aagagaatgt gaagcaagca
     3901 ccgactagct ggaaggagct gcaagagggt gactatcaag ttagcgtggg ggatgtgacg
     3961 attgccaacc aagcacaatt tgctgtattg gctgcggcga ttgccaacgg aggtgacgaa
     4021 aaggatattc agccaggtct gaattactac aaaaaaatgg cagaaaaagg ccgtttgtcc
     4081 accgttgatg ccagcgtagc aaatcttgaa aaaggtgaaa tcgatgtggc agttgtttgg
     4141 gacttcaatg gactaaatta tcgtgaccaa attgactccg agcgttttga ggtaactgtg
     4201 ccaacagacg gctccatcat tagcgggtat acaaccttaa tcaataaaaa tgcccctcat
     4261 cccaatgcgg caaagctagc acgggaatat attttatctg atgaagggca aatcaatttg
     4321 gcaaagggct atgcgcgacc tattcgcaag gtagagctgc ctaaagaagt agctgaaaaa
     4381 ttactgccgg aggatgctta tgaagcggcg gtgccagttg aagacaatga agcttggaac
     4441 aagacctctg tagagcttcc agagctttgg caggcggagg tactgaccta tgcaaagtag
     4501 acttgacaaa ctgattgtag ttatactaga cggctgtcgc tacgacacag ccgtcgccca
     4561 gcttgggttc atgaatcatt tagttgagca ccagcaagca agcttattga aggtaaaaag
     4621 tgagctgccc agcaattcac gtccgttata cgaagtactg atgaccggcg ttccagccta
     4681 tgaaaatggc atctatagta attaccatgc tcagcgatcc aaagagtggt cgctgtttga
     4741 attagttaag gaagctggcg gaaaaacagg agccgccgct taccactggt acagcgagtt
     4801 gtacaatcaa gccccctata atcgtttggt tgatcgcatc cagcatcagg aggagcgact
     4861 gattcagcat ggcatctttt atagtgagga cacttaccct gattcgcatt tatttgcgga
     4921 tgcccattat ttagtgaatc agtatcagcc agactttttg gtcgtacatt cgatgaatat
     4981 tgatgatgtc ggtcacaaat ttaccgccga ttctcatgaa tatgcagcag cggttaatca
     5041 ggcggacaga ttattggcta tttaccttcc tgaatggctg caaaaaggtt atcaagtcgt
     5101 ggtgactgcg gatcacggga tggatgcgca cggactgcat ggcggcagct tggctgcaca
     5161 tcgtgaagtt cctttttatt tgatttcaaa taaacgccaa aagctgaagg aacaaactgt
     5221 accacagctg ctggcggctc cattgttttg ctggttatta ggaattgcac cgtcagagaa
     5281 aatgcaggac attcaagaat tgatgagggg atgaaagaga tgaaaaaacg cttggttctg
     5341 gttcctgcct cctttatctt ctgtgcattt ttactgctgc cgctgctttt tatggttctg
     5401 tgcagcttgc acagtgattt aactaaccag tggacattgg ccaattacag tgatattttc
     5461 agcaacaaat attacacaca ggcctttgtt aacagcgtac tgattgctgt cagctccagt
     5521 gtgattgggt taatcgggac gtttatttta tgtttatgct taatgaatct atcgactaaa
     5581 ttgcaggaga aaatcacctt tgtttccaat ttggcagcta attttgctgg gattccgctg
     5641 gcattctctt ttattatttt gttggggaat gtaggtgtgc tgaaaatgat tttgccattt
     5701 ctagcggact tcaatttgta ttcttggtgg ggattgacca tcacgtatac ctatttccaa
     5761 attccgttag gggtcttgtt tttgtatccc agcatgaagg aagtgaaaaa ggaatggctg
     5821 gagtccgcac aattgctggg cgcttcatca gcgtatgctt ggttcaaggt ggctttgcct
     5881 tttttgcgac catcgattgc cagcaccttt gtcatcttat ttgctaatgg catggggacc
     5941 tatgaaacag cgtatgccct gaccagcagt aatgtaaatc ttttaacgat tcgaattgcg
     6001 gctttagtgt cgggagatgt gttcgccaag cctaatttag gcagtgcgtt ggcagtcgct
     6061 ttaggtgtcc tgctggtaac ggcaatgttg attattcaaa agaaaggcaa ggtggtgcag
     6121 acgtgaaaaa gttaataaaa ccgattttgt tctgctttgt cctgtattac ttgctgccga
     6181 ttattggaac gattttgtat gccagctcca ctaaatggtc aaaatcgctt ttacctagtg
     6241 attttacctt gcaatggttc caacagctgc tgacggacag ggaatttatt gctgcggtgg
     6301 gtcgctcact tttattagca ggtgtggtct tggtgacgat tctgttgttg atgattccca
     6361 ccattatttg gattcatttg tatttccctc gtttgaatcg ttggctggaa aagctgctcc
     6421 tgctgccgta tgctttacca ggagtgattt tggtaacggc tttgcttcga acgtatgctg
     6481 aaactggtat cccaatgttt gtagtgttgg taggtgcatt gtttattacg gcgttgccga
     6541 ttgtctatct tagtcttaac aaccagatgc gcttaattaa tctgaaggaa ttagtggatg
     6601 cagcagaaac cttgggcgcc ccgatgtcta cgattattat ccaggtgctg tttccaaaca
     6661 tccgtatcgg tgtgacccta gtttctctga tgattttttc ttcggttttt ggggagtaca
     6721 tgctgacgaa cctgttgatt ggcggtcgat ttcagacggt acgcatctat atgctgcggc
     6781 ggatgaacga aaacggccat ttggctagcg ctgtgatggt tttgtatttg ctgtttatgg
     6841 tgggaattgc tttaactacc tttatgctga cttatcgaca aaagcaacag ctgtccctta
     6901 agcagacgag ggaagagaag cagtcgaaaa agaagccctt aaaggagttg tgacggaaaa
     6961 tgtttttaga aatacatgat ctcaatctgt tttttgatga aaaacagatt ttagctattg
     7021 atgaaattca gattgaaaag ggtgagctgg ttactttact aggtcccagc gggtgcggaa
     7081 agtctacgtt gctgcgctgt atcacgggat tggaacaacc aaagagcggt gagattgtgc
     7141 tggacaatga gaatattgcc gataagccta cgaaggatcg caatatcggc tttgtttttc
     7201 aacagtatgc attgtttcca actatgactg tttttgaaaa tgtagccttt ggtttgaagg
     7261 taaagaagct gagtcctgag gtgattcagg aaaaggtttt tgagatgcta tcattggtgg
     7321 atatgacgga gcaggcagat aaaaatgtcc agtttctttc tggcggtcaa cgacaacggg
     7381 tggcactggc tcgctcattg gtcactgaac cgaaagtcct gctgctggat gagccattaa
     7441 gtgctttgga tgcccgaatt agaaaacagc tgcagcggga tttgcgcgct atccagcaat
     7501 cgctagggat gacgatgatt tttgtgactc atgaccaaga agaggcgatg cgaatcagtg
     7561 atcggatttt tgtgatggaa gctgggcggg tggctcaggt ttccacctcg aaggaattgt
     7621 atcagaatcc tcagagccga tttgttgcgg aatttatcgg caactataat cgtttcgaat
     7681 ggtatgagct gaatcagtat acccacgagt tttccgctaa gggcaatcgc tgcatttatt
     7741 atttaagacc ggagctgatt caatttgaac cggttcctca gggggttaag attccggttc
     7801 tatggaagga atcatttatt ttaggcaaca ttcaacggta tgtttttcaa acgctggagg
     7861 gcaaagaaat tttagtggat cggttgaacg atcgggaaga gaaaattagc gatgccttat
     7921 acattcagct gaaagatatt ctagcaatcc ctgtggaaat tgatactcag cattttgcac
     7981 aacaaagcta gtgtatagtc ggcaaacatt tctggctctt tgtcaaatag gactgataga
     8041 gtgatataag aacatctaag gtagaattga ttttctgctt tagatgtttt tttatgttta
     8101 ataagtgatc agatgattga ttaagaaaat tctggttctc atcctaaccc gtaagctatt
     8161 cgtttcaatg ctttgatgtg ggtatgcaga ttttctattt tgccatttga gtaaggcaac
     8221 tctaaggcta aacgaatgcc ggattcatag tttataaacg ctttttttat atgatgcagt
     8281 tctcttggaa ccgacttggg cattgttttt acaagctgaa agaagatgtc cgcttctttg
     8341 tcataaaaag cttgaagaat ttcttgatag taagcataag ctattcgtag ttcatcagaa
     8401 gtagaaagta gacgttctac gacttcactt tcggttaaat aggcccaacc aaaattacgc
     8461 cattttgtcc atttatcata aacaagcgtg tcctgtcgtt tgatcagaag tcgagataaa
     8521 gccttcactt gtcgatagtg tttggcttgt attggatcgt ttcggtcgaa gcttttcatg
     8581 actcatagtc aaatatcctt aaaactgcga atgaggtgct tgacgatgtg aaagcgatcc
     8641 acaacgagtt tggcatgagg aaagcactct tttatcagca aagcataaga ggcgttcata
     8701 tctgttacga gtatttttac gtttaaacgt gcacgtcgtt caaattgcat aaaataagaa
     8761 atgagatctt tcttcttacg tgaaggaaga atatccagta atttcccagt atcgccatcc
     8821 atacaagaaa aagaataggg accgacttga tttttcaatg aacgaaattc atctaccaat
     8881 gaacagcgag gcaaccaatt tattttcacg tttgtcatgg ggagcagaga agcgagtgtt
     8941 cgcgaaacgg ttgaccaaga aacgccgcaa tgtttagaaa tcgtcgtcat tgcttgtttt
     9001 tcagccaatt cctccaatat tttataatgt acttgtttgg cgataaaaca gtttttttca
     9061 acaatgtagg tattcgccgt aaaataggtg tgacagccac gacagaggta ccgttgcttt
     9121 gccaaagcaa gataagtccg ttgattcgca cacttattga gaaggaccag cgattttttc
     9181 tttccgttct taacgatcag cccatgttga ttcacctgtt tacactgagg acagggctga
     9241 atcgttttaa tgagcgtggc agaaacaacg atactgttga ttgaatgtat ttttttcttc
     9301 tcgacatgag tgatttgaat gtttccatct ttgattccta gtaatagttt gataagatga
     9361 agatcaggca tacagaacgc tcctttgatt gtgttgtggt gattcaattc taacggagct
     9421 tctgtatgtt ttgttttttt atgctcaaaa ggactgatag aatgatttac tatcagtcct
     9481 agaaattata gcgccacatt tctttcacct ttgacttttc agacggcagt ttttatgctg
     9541 atactgtaga gagagcagga cacagctttt gcttgttttg ggtaaggggg aaagaagatg
     9601 accaaagcac ttatttttga ttttgatggg acgatgattg acagtgaatg gatgttttac
     9661 gagagtattc atcactattt actgaagcct atttactgga gcagttcggc tataaaattc
     9721 cttttgcaga ctatcaacaa ggtgttggga cggcagcaga actgtccttt tatgaaggga
     9781 taagtcaagc ggttgggaga ccagtggatg ctgaacattt tgagcagcgg atcagcagca
     9841 ctggaaaatc ggagtagtct caattctgaa ctgcgggagc tagactattt ttttgaagca
     9901 catccgatga ttaaggaaaa aattgattcc tttgtgacga tcgatgatct tcaggtcggc
     9961 aaaccagatc cagaaggtta tttgttgtgc ttgaaacggc tgagggttga acaggctgtt
    10021 gtagttgagg atagtccagt cggtgcccaa gcagcaccgc atatgacagt tgatgtacaa
    10081 gcgttggatg cagatttttt cgcctttagc gggcataaaa tgtgcgggcc gacaggaatc
    10141 ggtgtattat atggcaaacg tcaattgtta gaacaaatgg agccagtcga attcggcggt
    10201 gaaatgatag attttgtcaa tctttacgat agttcatgga aagagctgcc atggaaattt
    10261 gaagctggta ctccgaacat tgcaggagca atcgattact tgaatgagat tggtatggag
    10321 aatattcatc gttatgagca agaactggtt gcctacgttt tacctaaatt acaagcgatc
    10381 ggcggtatca ccatttacgg accgcaagat cctgaacatc atactggcgt ggttgctttt
    10441 aatttggatg gcatccatcc ccgtgatgtg gcgactgctc tggatatgga aggtgtcgct
    10501 gtacgttcag gacaccattg tgcacaacgg ttcatcgacg atttctgttt gactaattgg
    10561 tcgtttaaac agtcgatgat aatgccgttt gagcggctcc aatgtcgtgg aatctttgag
    10621 taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag agtgcgaggc
    10681 tcgaaaacga ttcatgatct ggatacgcaa ttgattgaag ctgcgattga tatgttggac
    10741 aatatgaaag cgatcagtta cgatctccgc atttggaaag accgttttaa gcagctgaca
    10801 atagctggca ttctaggtac ttgaccttta aacgggcttt tctaggaaag tgcaggaagt
    10861 attgagttag tttgtaaagt ctgcgatctt caaggatcgt aaatagctga tgggttgtcc
    10921 catcaacaca aataaaactc atggaactag aaacagactt cattgatttg aactcatcca
    10981 tacacaacac cgtcggaagg aagttcatac gtggctggta atgcttcgtt gcttcacgca
    11041 agatccgtgt caccgtccca tccgagacga agtacttctt agcgattagt ttacgtgatt
    11101 gattttcgta cagttctatg agaatcgcct gcttcagttc ctttgttagt tgatgatgtt
    11161 catcaactaa gtccgttttc gcagaaaagg tctgcccaca atcatagcat agaaaacggg
    11221 tacgttttaa ttctaaaata gtcaactttt ctttgacttt caacatctga gtacgtgtcg
    11281 tataggaacc gttgcggata atacgaatcg atccacagtg ttcacaatgg gaaggtgagt
    11341 catccaatct ccctttaata aactgcgctt gaacgccttt atattctttt tcaatcagcc
    11401 attcttcatc aaaaaataaa tgtttatctg ttaatccgag taattttcta gtatgattat
    11461 ccatagagcg actcctttgg tagggtgatg ttttggtcga ctaaatttta ccaaaagtga
    11521 gtctcttttt gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt cgattttcca
    11581 acaacacaaa atattataga agcctttttt ctaaaggatt tcaaggtgtc tgtttgcatt
    11641 tattaaaaaa aagaaaaagt attggcaaca gaaggcgtca ccaacactag atattataga
    11701 gccataatat gctgtataat atctagtgta taggcttaag tggaggtata tatgaaaata
    11761 ttaactgttg aagatgataa tatgataaga gagggaataa gtgaatatct ttcagaattt
    11821 ggatatactg ttattcaagc taaagatgga agagaagcct tgtcaaaatt taatagcgat
    11881 ataaatttgg ttatcttaga cattcagata ccttttataa atggtttaga agtgttgaaa
    11941 gaaattagaa agaaaagcaa tttaccaatt ctaatcttga ctgcatttag tgatgaagaa
    12001 tataaaattg atgcatttac taatttagta gacggatatg tagaaaagcc attttcattg
    12061 ccggtcttaa aagctagaat agattcttta attaagaaga attatggaca tttagagaag
    12121 tttgaatata aggatctatc ggttaatttt aatagctata cagctaaaat aaatggtgaa
    12181 gaaatagatg taaatgcaaa agaactagaa gtattaaaat gtttattgga taataatgga
    12241 caagtgttaa caagaatgca aattattgat tatgtatgga aagatagcga agaaactcct
    12301 tatgatcgag taatagatgt atatataaaa gaacttagaa aaaaattaca attagactgt
    12361 ataactacta taagaaatgt aggatataaa ttggagagaa aatgaaaaaa ttaaagatat
    12421 ttccaaaaat gttc
//
LOCUS       DAJSVF010000073        12403 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSVF010000073 DAJSVF010000000
VERSION     DAJSVF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.076"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..503)
                     /locus_tag="ON550_002089"
     CDS             complement(<1..503)
                     /locus_tag="ON550_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR3955162.1"
                     /translation="MKLLNPKTVIAGTFAVTLAATLALAGSVDAASTSVMYRLYNPNN
                     HEHFYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEV
                     KWLTKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWK
                     YEGIGWYG"
     gene            complement(647..838)
                     /locus_tag="ON550_002090"
     CDS             complement(647..838)
                     /locus_tag="ON550_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955163.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            1026..1481
                     /locus_tag="ON550_002091"
     CDS             1026..1481
                     /locus_tag="ON550_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955164.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            1478..1720
                     /locus_tag="ON550_002092"
     CDS             1478..1720
                     /locus_tag="ON550_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3955165.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            1738..2040
                     /locus_tag="ON550_002093"
     CDS             1738..2040
                     /locus_tag="ON550_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955166.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            2109..4163
                     /locus_tag="ON550_002094"
     CDS             2109..4163
                     /locus_tag="ON550_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955167.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            4163..6775
                     /locus_tag="ON550_002095"
     CDS             4163..6775
                     /locus_tag="ON550_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3955168.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            6772..9150
                     /locus_tag="ON550_002096"
     CDS             6772..9150
                     /locus_tag="ON550_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955169.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            9211..9753
                     /locus_tag="ON550_002097"
     CDS             9211..9753
                     /locus_tag="ON550_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955170.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            9831..10163
                     /locus_tag="ON550_002098"
     CDS             9831..10163
                     /locus_tag="ON550_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955171.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            10164..10757
                     /locus_tag="ON550_002099"
     CDS             10164..10757
                     /locus_tag="ON550_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955172.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            10779..>12403
                     /locus_tag="ON550_002100"
     CDS             10779..>12403
                     /locus_tag="ON550_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3955173.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLA"
BASE COUNT         4500 a         1957 c         2527 g         3419 t
ORIGIN      
        1 ccataccaac cgataccttc gtatttccag ccatagtctt tggttaacca ttgtacttct
       61 ttccaacttg cagtgtagtg atgtgagcca gattttacac caggaaggaa aaggcggtag
      121 atcggtttat tatttttttc ggcagaacgc caggcaggcc cttcataagt ccagccgtaa
      181 tttttagtca accatttcac ttcatcccaa ctagctgtgt aatggtgatc acgaagacct
      241 ttattgtata aacgatagac aagatgacca ccgctagtcg gtgcttccca agcggggcct
      301 tcataattac cccacttaat tttcactagg tgatcacgtt cttttgtgct agaggtgtag
      361 aaatgttcat ggttatttgg attataaagg cgatacatga cgctagttga agcagcgtca
      421 acagatccag caagagcaag tgtggcagca agtgtgacag caaatgttcc agctataaca
      481 gtttttgggt ttaataattt catgttttca actcctatta aatgattaaa tattttgttt
      541 acattcataa tataccacgg tataccgagg cagtcaacgg tataccgtgg aaattttgaa
      601 ataaaaaagc cgtcctatga aaagtgtaag gatggctgtt tatatgctat gaagaaatgg
      661 attcggcgta tttggttagt ttggcagcat tatcaaatcg cattttttca atcgcacttt
      721 ttttctttat taaatccgca attgttgatt ctgcgactga agaatgtttg ctaatttcat
      781 atgctgtctt gccagtttca aatagtaacc attcgatttt ctctttatca gcgtacattg
      841 caatgctccc tcttgatttt ttctttatta tagctctttt ttgtaataaa tcacaatatc
      901 aagaggaaaa gtttctatag aaaggtggga aattatgagt atccgttccc cgtgtatcaa
      961 aatgaggttt gttgctactt tcgttattta atatactaaa ataaaacaac gaaagaagga
     1021 atagaatgaa aaagttaata atggtattta cttcgttgat tgccatgatt gttattttag
     1081 gtggatgtgg tagtgaaaaa aataagcaag atagtcaaga taatggcgtg ttaaaaccag
     1141 gaacagtgtg gaaagaagaa gtaggcggac ttgttcataa tttaaaaatt atagatgata
     1201 caacatggga atattccgaa agtgtttggc atcctgaaca agtacaaata acagttgaga
     1261 agctaaaaga ctataaagga ttagaaagat ataaggtaat cgattcaggt ggagtagatt
     1321 tattcatagg taaaacagcc ttaatagttc cctataacaa agacggttta gaaacgttta
     1381 aatttattcc aacaaacgaa aaagaacatc gagataaaag tgaaatatta aaagaataca
     1441 gtactaaatc tggatacaag ttacaaaaga ctagcgagtg attgctagtc tttttattta
     1501 tcctgaagga ggaatcgcag tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa
     1561 aatcttagaa cgtcgcggtg tatctgaaac acagtggaaa caagaagtcc ttgaaaaagc
     1621 acaatttgac ctattacggg gaaaagacaa ggacttagaa ggatatatct atcaacgtga
     1681 acgtgaaaag cttgttttcg atgaaattat taaacactaa aaggagcgaa caattttatg
     1741 tttgaatcaa ttacaatgaa ttttttaaca caaccgaaag tagtagctgc ggttgatatt
     1801 tttagagcat tacaaggtgc agcaactagt ttaaaaaaca atctgatgat ttttgcggct
     1861 gtagctgccg ttgcctccat tgttcttggg gggattttct tcttctttgg ccgaagagga
     1921 gctgaaaacg ggaagatgca cgtaggttat gtattgatgg gggtaggagt tatattagct
     1981 gccgcctcta ttgtggtttc gttatttgga atgtttggac aaaccccaca gggactataa
     2041 aaaataaata gaaggagtaa aaagtacgtg gctacttttt actccttttt tatgaaagga
     2101 gaatactcat gtactttacg atttcaggca ataccaaaaa agataaagag ttaataaaaa
     2161 aagcaacgaa gaaagtggga aagcgtactg cctttttacc gaaaacaatt aaatattctg
     2221 tcattgaaac ggaagaacag ctacatgtgt taggacaagt gttagaacaa gagaatcaat
     2281 cttctgttaa attgactctc acgttacatg ataaagaagg aaaaatagaa aaggctggca
     2341 cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttttat cctacattga
     2401 tggatttgat ccgccttgat gtgtttaagg aaacggatga aaccatgagt gtggaagagt
     2461 cttatcgaaa cgaaaaactg ctacaagttt tagaagaaga gttagagagc gaccaaagaa
     2521 aagtgagtca agtgagtgaa gtcgaagtaa atgatgaagt accatgggaa cgaccaatct
     2581 ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa gctgatattc
     2641 cgactcagaa atcgatattt gaggaagaga acgatgtaat gggtcaattg gaggtagatc
     2701 cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat ctagttgaag
     2761 ctaatgaaga gccaacatgg actgaagggg attctcagga atatattgaa ccacttatca
     2821 gtgaagaagt agagaaaaac gaaacagaga tacaaaaggc aactgtattt gattatattt
     2881 tggatcaata tccgccagag catgaatgga tgaaagaaaa aaataaatta tttttaaatg
     2941 agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag agtaaagaag
     3001 aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtt caatcaacaa
     3061 attggcatga gcttgcacaa gaagatttat tagccacttt tgaagaacgt caacaagaaa
     3121 cagatcaaga aataaatgag tatgcgacgg aacagtcatc taattgggag agaacaaaag
     3181 aggaactaaa taaagaagaa caacgagtga ttgaagaaga agtacaaaaa attaaagagc
     3241 gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtat agagaaaatt aataacttta
     3301 ctaaaactaa tcaagaagct ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtataa
     3361 aagaacgaaa agaagagttc taccgaggtt tacgtacaga taagctaaag attagcaaag
     3421 aattaaatga acaactgaat aaactttatc aaacaacgac tgaagttctt atggaaagac
     3481 acgggcagat ccagcaagaa ttacaagcac aaattccaaa atggaaaaaa gagtatgaaa
     3541 ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg acaagaagaa aaagagcgcg
     3601 aacaattaga actaaaaaaa cgtgagcagc atcttgcaga acaaaaagtg gcgatagaaa
     3661 aacaacggtt agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac
     3721 aaatggatac attgcgacaa gcacagttga attatctagc acaacctcaa ccagtagctg
     3781 ctgttcaaac gccagtgaac aaccaagcaa atttaactcc ttcaccacag cgttccttta
     3841 acggatggat ggtgggatgt attgcaagtt taagcttgct tctaggtgga ggaacagtat
     3901 ttgcttttaa tcatatcaat caatcttcac aggcagattc tgtagccaca acggcgtcaa
     3961 tcaaggaaga agcccaggaa caagcaagaa aagagtatga acaacaattg gaagaattat
     4021 tgaatcgtac caatcagtca acagcagagt caagtatgga aaatagcaaa gagagtgatc
     4081 ctagtaaggc taaagaagga tcaagcgatt caactgtaca ctcaaacact tccgaaagtt
     4141 cagaatcaaa tgaggtgaaa taatgcgtat tagagaaaca acgagacgaa aagacaaacg
     4201 attcaattta gaaaagaata ggtatctttc tcatttctat cgctatactc cttcaggaga
     4261 agagctacca gaaaaacatg acaaacgtat ttctcaaaag attttttata gtggcttagc
     4321 ttatcttcct ttaaactatg ttgttacaag aataccagaa gcatttcatc gatttgagat
     4381 tattccagga agtttacaag gagtgaatat ttttcagtta caagaaatgc cgcagttatt
     4441 ccattttgat tcaactggtt tttggaatcc tttgacccca acaacaagtt taggcacacg
     4501 tttagttgtt tctacgattt gtgttgttcc agctgtactt atgggattga aaatggacat
     4561 gtggtttcgt ccgctagcag atgggcaaaa aggagataac cgattaaaaa cgttaaagga
     4621 aatccaacaa atttatccgc aaataccaga atcaaaggga agttttcctg gttatggcgg
     4681 aataccaatt actcattata aaaaatactg gtatattcag acggatacag taaatacttg
     4741 tattgttggt acttcccgct cagggaaagg ccagatagaa gttttagcaa ccattgacaa
     4801 tttgtcgcgt gcagaaagac aaagttcaat ggttgttaat gatccaaaaa cagaattata
     4861 tgttgctagt aaagatgaac tagaagctag agggtatgat gtgtatgcct ttaatatcac
     4921 cgatccactg caatcaatga gtgataatcc attggcactc attgtgaaat actggaagcg
     4981 aggagacata gacaccgcaa cacagttaac caatactttt acgaatacga tttattatga
     5041 tgcaaatgct tcagacaata aatactttaa cgaaaatgcg cagaaagctg tcaatgcgct
     5101 tatttttgcg ttattggaac aagcggatca aacaggggat tactccaaat taacaccaaa
     5161 taatctcgtt gaactacttt cagaaattgg cggttttaat tattatccag atcctgagaa
     5221 tgaattcaag cagctcaatg ccttagatga gttcatgaat cagttacccc ctggaaatat
     5281 agcaaaaaaa caatatgggg caacaaaaat tggatctgat aaagcgaaag ggaatatctt
     5341 gtctacggca attacaggat tgaatccatt taccctaacg aaaattgcga agatgacttc
     5401 tcaatcgacc attgattaca agtccattgg ttttccgaag tatttggatc tcaaattaca
     5461 tgaaagccta ttaaatcaac gcattgtcgt ggaattttat cataagggaa agaaatttca
     5521 cgaggaacga gtcaaagttg gctatcgtgg tttttgtgaa atcaactttg attgtcattt
     5581 agagcaggga gatcatgttt gcttaaaata caatcatcaa ggaaaacaat atgttgcttg
     5641 gtatgagttt tctcaaaaag tattgaaaga cgaagaggga aaaatcgttt atcgaaaaaa
     5701 acgaggagaa actcatttgc cagaactaga aaaagaagcc gttctaacat tagatgagaa
     5761 gaaaagtaac ctattagtag agtctattcg gatgcactat accgataaac caatggctat
     5821 ttttatgtta acgcctgatt atgatccatc taaccatatc attgttagca tctttcttag
     5881 tcagttgtat aaagaattat ctacgcaatg tgttaagact aaaggagata aatgtcaccg
     5941 taggattcat tttattttag atgaatttgg aaatatgcca ccgatggaca acatggaagg
     6001 aatcatgacg gttacggcag gtcgtaatat gttatgggat ctgtttattc aatcttatca
     6061 gcaactttat gcgaagtatg gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac
     6121 acatatttat attatgtcga cagatgataa taccatcgaa gaattttcta aaaaagtggg
     6181 gaataagacg gttgaacaag agaacagtac catcaattca ttaggtatga atactcatat
     6241 caatcgcaat gttgattcag atcgtatctt aactcctgaa agaatttcta cttttttaga
     6301 aggtgaaacg attgtactcg ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg
     6361 gccgtatcct atttttaatc atggggaaac gaatcttcct tatgcttatc aatatattga
     6421 agatttcaat ccatcgtggg atatcaatga tatagacata cattgtacac atgcggactt
     6481 ggatcttaag gctttacaaa ttgattatga ttatttccta ccagatgaat atgctagaaa
     6541 tctctatcat ggactgcatg atcaattaga agaaaaaaac gaagaaaata tgttttataa
     6601 aatcgttcaa tcttttgaga atgagacggc taaaaaattg ttaattcaat tttacgaaga
     6661 acaagcaacg gatcgtcttc aagaatatgt tgaaaagttg atcaaaaatg aagaaatttc
     6721 tgaaatgatt aggaaacggc tgttattgga agctgaaaag gcggtgaaag aatgatcatt
     6781 ggaggaattt tctctggcat agccgactgg tggtcaggtt tattcggaga aggggatgca
     6841 gatcccaaaa ccgtaacgaa agtcttacaa gattatggta attatctgca gtatcaagat
     6901 gtattcagtt ggatttgtaa tactttttta tggggattga tcaaagggtt atataatctc
     6961 aatactttat tagaaaaaac aatttatcaa agctttgatt taaaagactt attgaacgcc
     7021 gctggtgtta atgaactatt tcaaagtttg attagtaagg tattggctct tttttgtgtc
     7081 gtcacattaa tttatttagg cttgaaattt tttatgagta aacacccacc gaaagtcaaa
     7141 aatatcttgg ttaatttatt tatggccatg atcttgattt ttggtggtag ttctttaatc
     7201 gatgaagggt taaatatcag taaaaacttt tatggagacg tcactgcagc aaataaagca
     7261 gataaaacgg gttcacctgc ttttcaaatt attcaaaata atgtgattga tgtcagtaca
     7321 ttactagcga ctgacccgaa taaagtagaa tccttaccaa cagataaaag aaatgcttta
     7381 acagcgaaaa atttcaaaac agcgaatatc aatgggatta ttacacctga tcaagctaag
     7441 aaaatgtctg aatcaagtga tattagtaca gtacaaaaag atcggttaaa ggcattacag
     7501 tatcgactag agttagacga taatgggaaa gaaatccctg ttcaaattag tgatgaaggt
     7561 ttagcaaaat atgtgtatac gtcaggctat cgtcgataca ttagtagtcc agggattatt
     7621 ttggctgggg aatcttcttt gtcggtagcc tatttattta ttttgtttac aatcgttact
     7681 tgcattattg agctagtatt taaaaaattc tatttagtaa ttgccgcgtc aacggacttt
     7741 gagacaggac aacggatgaa aagtgcgata gaagatattt ctcaatcctt cttgttattg
     7801 gcgtttacga ttttagaatt acgaatctat acattaatgc tttcaggtat cggtgatttg
     7861 catgcggtag gaaagatcaa tggattcttg tatgttgttg ctttaattgt gctaactatt
     7921 gcgctattca aaggctctca atcggttacg aagattttcg gtgttgatac gagtctgaaa
     7981 aacggcagca attctcttct tagcatgttt gccttaggaa atattgcgaa aaatgtgggt
     8041 agcggagcta aaaaccttgc aagtgctggt aagagtggtt taagccattt aaatgatatt
     8101 cgtaagaatg gatttggcca tcgaaaagat attacccatg ataacgggga agaacaatca
     8161 gacgataaag ctacaactgt acctaatagt aagaagacag tttccgaaat gttcgggaaa
     8221 gcgaaaaatg gtgtttctaa agccgcagaa ggtcttggtt acgtcaatga acgtgggtta
     8281 aaaggaacag ttgaagatct tacagaaaaa gccaaagaca ccttagaaga gaaattagac
     8341 ggcacgagag ctaaagaatt tgctgaagct gtgaagaatc catccgaagc actagaaaaa
     8401 caaaaagaaa aagcttcagc agtcaaagaa ggactaaaag atgcgtatgg tgaaggacaa
     8461 gtcaatgcag cagtgaaggc aaatacaaca agtgaaaaag aaaatcaaga aggtgcagcg
     8521 attccttcag ccaaagatca ccaagatgag ttacattcta acgaaaattc gatcggagaa
     8581 aaagattcag aagaaggaca acatggtcag ttaccaaaaa ccgatccaga acaatatcgt
     8641 tataaatcta acattaattc tgaaaacgaa aaacgcattt cagatttacc aaaacgaaat
     8701 caagaagtac aaacggatga ggattccatc gatctgccaa atgataatgg catcgaagtt
     8761 ggaactgaaa aatcaacgaa aattggtttg cctaagacac aaaatttacc aatgaaagat
     8821 agtcgtttga atcaattcaa agagaatgat caaagctttg ttcatcaatc acctgcaggt
     8881 actgttcaaa tgaaatcaag tcgtttgcct gaaaatggaa atctgacaac tgtttcttac
     8941 aatttagctg aaagtggtga aacagagaat gcgtccgttc aaaatattgt gacaaaagaa
     9001 gctgcaccta agccaatcaa taaagttcat aatggataca gaccagcaaa cgtagcattt
     9061 actgcaccga caaaaataaa tgtgacaaga gaaactaaaa acaggaacaa tgaattgaaa
     9121 gaacaactat tgaaggatat caaaccataa aaggcctatt taagggcctt tttctttata
     9181 aaacaagttt aaaatggaaa ggagaaaatt gtggaggata ttttaatagc tacgtttata
     9241 actgcactgg gagcattttt aacgatattt gtggtgacta ttaaaggatt taaagatacg
     9301 gatagtttac aaaaagatca tcagtctatt ttagatggtc aaaaaacaca taccgaaagc
     9361 attattgtac acctttcaga aggattcact tatttgaaag atgctatgcc cacattcaca
     9421 gtaaaattaa cggaaatatt tcattctgtt gagcaaatat cgtcagaatt gaaagagcaa
     9481 agacaacaaa aagatcgtca acttggctca cttaatttta ggcaagaaac gatacaagaa
     9541 tcattggaga atgtaaatcg attaaacgat gaaatgcaac gagttcattt ggaaaatgaa
     9601 gaattacgcg cacaaaatta tgatttaatt caaaagaaaa gaaatctgga acaaaaaatt
     9661 gttgaattaa aagaagaaaa tcgtcaattg aattatcaat tacgacgagg aaaaaatcgt
     9721 tctcgtgatt taggaaatga attagagttg taatatttag aaaaacgctg tgagaaatca
     9781 cagtgttttt tatttctaaa aatacaaaat ataaggggta tggttatatt atggataaat
     9841 acattcatat gaaaaatcct ccaagattta gtggggaatg gaagtttttc gcctttttct
     9901 ttttagatct attaacgctt ggtggctgct ttttttgggc gaaaatagtc aataatcttg
     9961 tccacttacc actctttttt gcactcttga attatggatt atggcttgcg atagcgttag
    10021 tgattgtttg gcgaccctat aataaccctg gtcaacgcca aatttttgcg attatcgaat
    10081 ctattttctt tacggataag aaccactatc atgcgttaga tcctaaccga aattatcatg
    10141 gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca aaattcagtt gataagaaat
    10201 ccattttgca gtttggaaaa ccagaagagc gctctatctt ggacttattt ccatataaat
    10261 cctgtacaga agagggagtc ttaattacta gagaagatca ttttcagcgt tattatcgtg
    10321 tcgtttccac tgacgtggag ggattaaatg aacaagagaa actagaacga atgaatcagt
    10381 tgaccaccgt catgcgtacg tatgtgccaa caattaagtt agttagtttg accacagaga
    10441 cggatctaac ggaacaaatc gtgcaaaagc ggcagttatt agataaaaat cgtatggaac
    10501 agtcactagg aagaaatcta cagcgattaa gaaaatatga aaagaaatta gtagaggaga
    10561 tccaggaatt aaaacgggca gaacaagaac gtcctgactt aagcttcttt ttcgttattg
    10621 aaggaaagac aatcaaggat ttacaaacta gaaatcgtca gttaatgcga tctagtggca
    10681 ttttagggtt aaaaccactc tacaaaaaag aattgattaa gattctttat cgaatgaaca
    10741 acatgaatga cgaataaaaa agatggagga aatgattcat gctagatgtg ataaaaacag
    10801 ctttttcaac gcaaagtaat caaaaggtga ttgatccatt aaaacaactt ggtaatcgtg
    10861 aaatagagat tttaaaatca aaaggttatg atttagattt cttaaaacaa gtgatgccac
    10921 aaggtgggtt gaattttgat gaagaatatg tgacctatgg aaatggttat tcacgatgtg
    10981 tgacagttta tcattacaca aaaaatccta ctcttttttg gttggccaat ctcatgaaca
    11041 atcctcatac agtggccaca ctagatgtta gcacagatga aaagcaaaaa gtgattgatc
    11101 aaatcaaccg cgcattagat gaattaaatg atcgatcaga taatgaacga aaagcgactg
    11161 accgaaataa aagtgcggaa gaatacctgg aactagcgga atatgcccga aaattgactt
    11221 ctaatgggga aatttctaaa caagtcaaag tgagagtaca tgtttacgga aatacgatcc
    11281 aagaagtgga tgaacgggcg caggaactga tcgaaaatct aaaagggatg gattatcgtg
    11341 cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc atgttcgatg
    11401 aacaagctga atggtttgga aatatcaaag ggcaatcact tccatcgtta aacattggcg
    11461 gaggattccc gttccaccac caaacgctaa aagatccagg aggcacacaa tatggggaaa
    11521 ccatgacagg cggcccattt gtctggaatg ccacgtataa aaatagtgta cgtttatcta
    11581 ccaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt aaaatgatcg
    11641 cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga aaagacttta
    11701 ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct gacgggatga
    11761 tcaatccttt agagatcttt gccacacgga cgtatgatga agcatccagt ttatatgacg
    11821 atggttcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaacg cacttagaca
    11881 aagtagtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat
    11941 tcaagaccta cttgaatcgt ttttatgaag agtatggaac agtaccacgc ggttttactt
    12001 cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca gaagcttatc
    12061 cgacgttcaa agaattttta gcctttttag gacaactgga actaccaggg gcttcccaag
    12121 agaaaaagaa tcggaaagaa gaaatggagt ctatcgtgga atccttatgt gagacttatg
    12181 gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag ttggtctttt
    12241 ttgacattga ctctattaca ggcctagatc gaggggtaca acaatgtcag atgtacatgg
    12301 ccatgacgtt gatttggaat caggcattga ttcaaggacg aagacaacga cgcttaatta
    12361 aagaaaaaca aatggaagaa caagacttca aacgcttctt ggc
//
LOCUS       DAJSVF010000074        12269 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSVF010000074 DAJSVF010000000
VERSION     DAJSVF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.077"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..572)
                     /locus_tag="ON550_002101"
     CDS             complement(<1..572)
                     /locus_tag="ON550_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3955174.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISDWY"
     gene            complement(572..1996)
                     /gene="istA"
                     /locus_tag="ON550_002102"
     CDS             complement(572..1996)
                     /gene="istA"
                     /locus_tag="ON550_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR3955175.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            complement(2391..3233)
                     /locus_tag="ON550_002103"
     CDS             complement(2391..3233)
                     /locus_tag="ON550_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3955176.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            3558..3974
                     /locus_tag="ON550_002104"
     CDS             3558..3974
                     /locus_tag="ON550_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3955177.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            3971..4441
                     /locus_tag="ON550_002105"
     CDS             3971..4441
                     /locus_tag="ON550_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3955178.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            4455..5228
                     /locus_tag="ON550_002106"
     CDS             4455..5228
                     /locus_tag="ON550_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3955179.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            5242..6054
                     /locus_tag="ON550_002107"
     CDS             5242..6054
                     /locus_tag="ON550_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3955180.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            6108..7073
                     /locus_tag="ON550_002108"
     CDS             6108..7073
                     /locus_tag="ON550_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3955181.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            7210..9933
                     /locus_tag="ON550_002109"
     CDS             7210..9933
                     /locus_tag="ON550_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3955182.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9946..10578
                     /locus_tag="ON550_002110"
     CDS             9946..10578
                     /locus_tag="ON550_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3955183.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            11477..>12269
                     /locus_tag="ON550_002111"
     CDS             11477..>12269
                     /locus_tag="ON550_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955184.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3977 a         2026 c         2378 g         3888 t
ORIGIN      
        1 tcataccaat cgctgatcaa ttggaaaagc agttccgcgc cttctttagt gaaaggcaca
       61 taacccatct catcaagaat gattaatttt actttatcga agcgatgctt gaagacctct
      121 aaggtattgt ttttccaatg gtgttccaat tcgttgacta aatctgatac ccgccaaaag
      181 cgtgtatcga tacctgattt ggctgccagc tgacccaatc cacaagctag atgtgtttta
      241 ccggtaccgg gtgtacccac aaatacagca ttttctttct gatgaataaa cgatagactc
      301 aataattcat ctttagtcgt cgcatttggc aaacatatat gatcatgcca ttcgtaatcc
      361 tctaaggtac gtttatttgg gaactttgct tgttttagga gtcgggcagt tttcaattct
      421 tcacggcaag ccaactcctt ttccagaaga tacatcaacc aatcttcttt ggtttcaaag
      481 ctgtaatcta agtctttgcg aaccaaagtg ggaagaacat gggaaagtct cagcaactta
      541 caggcagttt ctaaactttt tgcttgatcc actagtcaat gacctcccct gttagagaca
      601 agaaagcatc gtattggtgc caatcaatat catacgcatc ggtagtagtc gaggcactaa
      661 acatgtcgta aaaatgtgta tcaatcccta acatatcatg tgtcaccaat aaacctgaga
      721 tttgatttaa acgcttctcc tgttctttcc aatctggatg actgagaaat aacttgattc
      781 gttcgggcaa atacttccaa tagcgagagt aatgcatggc acgcgatttt ctgcgccaat
      841 caggaagaat tgcttgccaa tcgattgctc gttttttcat catataagga cgaagtcctt
      901 ctgaaataat ctcaccatca gtgctgatta gctgatagga atcaaaacga agcagcccat
      961 aaataattga ataattgcgt acacgtggaa cgtggatccg ttcgttatct aatttaattt
     1021 cgttgtactt attgactttg acttcgattt ctttaaatac tgggtaatcg gtgtccggca
     1081 aagctagcaa atctgttttt tctgtgttcc atagatcatt gataatgaca tgtttttcgt
     1141 aatggattcg ctctcgatcg tgtgacagct ccaccgctaa tgcctcgttg aaactggcga
     1201 aatccttcat aatcggtgta gatgaaaaga agttgtaccg tacatagccg actttgtttt
     1261 ccatactacc tttctcgttt ccgctacgtg gattgcacac ctgtgtctca aaaccatagt
     1321 gcatagaaaa tcggaggaaa ccgtcagtca gcaccgcttg ttcagtcttt gtttttgtct
     1381 gagtaacggc cgttgacata ttatcaatcc gtattttccg aggaacgcca cctgattggc
     1441 tgaagagttc tttcatgcct tccaacaaac attcttgatt ctcggcgggg agtacaacgg
     1501 cgaatcctgc attactaaaa gggaaagtca tcactaatac cttcacatct ttaaacgctc
     1561 catcgtgaca aacttccatc gtaccaaaat caagttgcgc ttctgctggc gggtgttcaa
     1621 gacgttcaaa gccttggtct tccagtgttt ccaagtagtg tgtcgctttc caatcttgaa
     1681 tgaatacaca gactgttcgg taggatcctg gaaagccatc ggcttttaaa gcagtagtca
     1741 ttgcttcagc agtcctacgc tttttctttg gcagcttagc atcttctgaa agccataggg
     1801 aaacgatgcc tccccacttt tctgtataca tcataccagt ctttttgcgt gctttaggtt
     1861 gcggcagcaa atcatcatct gcatattttt tagccgtgcg ccaatttaca tctagctcct
     1921 ttgcaatatg attaatggat ttatcatgat tatttcgtaa atttctgata gtattaatat
     1981 cggtcattgt gagcatacct ttcttacctc cagttacagt gtcgagacta taactttaga
     2041 ggaatttcag gttgcccgca atggcttttt atcttaactt aatcgatttg cggagttgtg
     2101 tagtttttcg ttgcacttgt atgtactttt attctgccat aaacagctta atgaatgtcc
     2161 acttgaaaaa gtgaataaac gaaatagatg actcgattta ataaggaagt aaacccacac
     2221 agaaatatag gtgattttcc ttgaatgacc aagatattga aagttagtga aaaaacacaa
     2281 aaaggtagcc ctattcacag aaggtctgcc ttttgtgtta tcgccagcaa acattagagg
     2341 ggatatttca tatagctttt gtcctctcga cagtcctatc agtttctctt ttaacttatc
     2401 tctaagtgct gtccagtttt aatagcttct agcttgtttc ctaactgtgt ttttaatatg
     2461 ttgaaggctt ttgaacctgt gcagtggcaa gtaaggattt tttctactcg tgagtcattc
     2521 aatttatttc caattttcat tacctcttgc tcaggtagac ctaaattatt tatataaggt
     2581 atgccaatca tatggaagcc accgattagg tattttatgg gtttagtcgg aaatgtttca
     2641 agagcaattt ccatcatatc gataatgcca tgatgactac agccagtaaa gacaatcaga
     2701 ccgtcttctt gctcaattac gaccaataat tcatctgaaa aatcatcata aacaaattta
     2761 tcatccatct ttttgtataa attcttggtg gtcgagtttg ctttgcctag ggggatgata
     2821 tggaagctcg gagcaagttc tgttttcgtt tttattgtat gtaaattctc atgcatttta
     2881 aggatttcat ttttaggaat accaacatac agaaacgtgg ttaaaatctt taaataatgg
     2941 ggtaaagtta caccttcagc tgtataaata ggggcgtttc ctgattttaa atatcttaac
     3001 ccgccggtgt ggtcagaatg accgtgtgaa ataatcactg cctccagatc attcaaagtt
     3061 tcccccattt tagtcgcatt atagactaat tttttattag cgccagcatc gaagagatat
     3121 ttcttccctg agtagttaat taagagagat aacccatgtc cacttttaaa gttcaaattt
     3181 attcttgagt tttcaaataa aaccttgatt tccactataa catctccttt cacatgaacg
     3241 tgtattcata tattgtagca cattaagttc tattttttgt tgtaattacc gaaaaaaata
     3301 ttaatggacg ctttttacgc tttatttcgt atgtggaaga aaatttctta ggatgtggcg
     3361 ggggattggg gcaaagctaa attccaatta cagtgatcat ttaattagac acctttaaat
     3421 acccacactt tcatcaacac tataatttag ggaaagaaaa gtgttggcat gatatagaaa
     3481 aacctttaat tgacagcgtt tcctaaaatt ggcacgcatc ttgcttatag aaaagcgtaa
     3541 ggataggagg aatgaaaatg gcaactataa ttcttgccag ccatggggaa tttgctcaag
     3601 gattaaagca aacagcgaca atgattattg gagatagtgt accaattcat gccttgtcag
     3661 catttagaga tgaagatgaa tctattttgg tacaaataaa aaaactttta gccacaatag
     3721 atatagaaga aacctacatt ttaactgata ttctaggtgg tagtgtgaat aatgatatgc
     3781 tcactattat taaagagtat gagggattgc aattaattac tggaatgaac ttacctttag
     3841 ttatttctat tgcaacctat aatggaaaaa ttggggaaag tgagctagaa aagcttatta
     3901 gagagagtca acaatcagtg attgattgta gaaaattatt agtagaacaa cttactggag
     3961 gagatgacct atgattaaat tagtacgtat tgaccatcgt ttgttgcacg gacaggtagt
     4021 attttcttgg agtaaatcat taaatatcaa tcgtattatt attgccaata aagaggcagc
     4081 gaatgatgaa ttcaaaaaaa tgtctttgaa tttatctaaa ccgactggca ttaaattaac
     4141 aattttttca gttgaacagt tactagaaaa aatgcctaaa atcgaaacac ttaacgaaaa
     4201 tatcatgctt atttttggta atacaaaaga aattcgtcaa ttttgtgaag cctatcctaa
     4261 agtacaagaa atcaactatg gagggataat caaaaaagaa ggttccaagc aatttagtaa
     4321 tgccattttc ttgaatgaat ctgagcaaga agatgccaaa aaattaaaag ctcttggcat
     4381 taaacaattt attcaacagg taccaacttc taaaaaagaa gatttgaata caatgttata
     4441 aaggaggaat gaaaatgctc gttcagtcaa ttggtttagg gttaatcggt gttttttgta
     4501 ttttagactc aaggctttta ggaagaatga attttgaacg accgttgatt gtcagtacgc
     4561 ttgttggttt ggtacttgga gacatggaaa aaggattaat tgttggagcc tctttggaat
     4621 tgatgtcctt aggattagta aatatcggag ctgctgcacc acctgatatg aatatggcat
     4681 caattattgc tacagccttt gccattttat caaatgcgaa cgcagaagca gcattaacga
     4741 ttgccattcc aatttcagtg ttaggtcaaa tactaggtat cttaatgcgg acgattttgt
     4801 caaatttgac tcacgttgcg gatcgcttta tcgaacaagg gaaatttagc caagcaagga
     4861 gaattcatat actttatggc tcgggattat atgctctcat gtattttatt cctatttttc
     4921 tagcaattta ttttggaacg aatcttgttt ctcagattgt tgatgcgatt cctgagtggc
     4981 taacaaatgg cttaacggtt gcaagtaaaa ttttaccagc atatgggttt gcgttgttat
     5041 tacaaacgat gttaacgaaa aaaatggtgc catttttatt acttggattt ttaattactg
     5101 cttattctgg attgggaatc attggtattg cagccttcgc atgtattgtt gcgtttgtca
     5161 tggttcaagt tcaaggtaaa aactcaaatg gtaaccaaaa tgagccagat ccattagatg
     5221 acttataagg aggtagatta agtgaaagag aaagatcaaa atgcatggaa atatcggcag
     5281 ttcttatggc gttcatgggc aattcaagcc tcatggaact atgaacgaca aatgaacatg
     5341 ggttttatgt acggaattgc accaattcta gataacattt acaagaatcc agaagatgaa
     5401 gcaatgaaga aagaagctta cgagcggcat atgatgtact acaactgtac ccctcaaaca
     5461 agtgcatttg tgttagggtt aactgcttct atggaggagc aatactacga agaccaagaa
     5521 aactttaatc cggaatcaat cagtgcagtg aagacttctc tgatgggacc gctatctggt
     5581 gttggtgatt ctttctttca aggaacgatt cgggtattag cctttggact aggaattaat
     5641 ttagcccaac aaggtagtat tttaggtcca attctagcaa tgttaatttc ctttgtacct
     5701 tcctttttag ttacttacta tggtgggaaa attggctata acatggggaa taaatacttg
     5761 tctaaactgt atcaagaagg tttgatggat aaagtaatgt atgtttgctc cattgtgggg
     5821 ttgatggtca ttggttcgat gattgccagc atgatcggga ttacaacacc aattcaaatg
     5881 ggtaaagctt ttgtcttaca agatgtatta gatagtatta tgccacaatt tttaccatta
     5941 ggattaacct tcttcatgta ctggttattg cagaaaaagg tcaaaacagg atggatgttg
     6001 gcaatctgtt tagtcggagg aattattttt agtttgttag gtatcttaca ataagggaat
     6061 aaccaataaa tttggttgaa taatataaat aaggaggaat tcaattgatg aattcaaaag
     6121 aaattgttaa agaaatcgta gcgaaaaaag aggtaaaatc aatcgtattt gttggctgtg
     6181 gcgcttcaaa agcggacctt tatcctgcaa agtattttct agaccaaaat gcgaagcagt
     6241 tacggattag tcattacaca gccagcgaat ttaactatgc aacaccagca tctgtagatg
     6301 agtcaactgt cgttatttca gcttctttag gaggtacgac tccagaaaca gttgaagcta
     6361 acgcagtagc taaagaaaaa ggtgctacag ttattagtct aacacgaagc attgactcac
     6421 cgttaacaaa agatgcagat tatgtaattt atcatcgatt tgcagagaac tatgctgcta
     6481 aattagaaaa aacaggctac tgtttagaat tggcagtaga acttttacat caagtagaag
     6541 gttatgacca ttatgaagca atgcaagaag gatttgctac tatctatgat cttgcccaaa
     6601 aaagtgcaga atcagctcgt aaagatgcga agaaatttgc tgaacgcttt aaagatgaaa
     6661 aagttattta tgtaatgagt agtggtgcta cacatgaagt agcatactca acatcaattt
     6721 gcttgatgat ggaaatgcaa tggattaatt ctggtacctt ccattcagga gaatttttcc
     6781 acggaccatt cgaaattgtg gataaagacg taccgtttat attattaatg aatgatggaa
     6841 aaactagaaa tatagatgct cgagcattaa ctttccttga acgctttgat acgaaaacag
     6901 aagttgtaga tgccttggat tatggcttgt cttcacatat tgctaaagaa gtcttagact
     6961 actttaaccc attcgtgatt actgcagtat ttagagttta tgcagaagaa ttgtcagaag
     7021 caagagaaca tccattaact aagagacgtt atatgtggaa actaacttat taaaaaatag
     7081 atcaaactaa ataaggtggc ttgagaaatt aagccacctt gttttcttca atgtgatttt
     7141 atacatatgg taaaataact agtacatatg gtaaaataac tagtacatat tgtgaaagca
     7201 ggtggcaaga tgcagcgatt aaatgactat ctttatcaac taacattgct tttgaaatct
     7261 acgccttcag ataattattc agctcgcagt atttcgcaag ctttacagtt gaatagaagt
     7321 acaatcagta gctacttaaa tgaaggtgtt cgtgaagggc ttttcataaa agtaaaaagt
     7381 tacccggttt tatttttaca tagaacagct cttgaagaac tacatatcac actgaataac
     7441 tccgaaatag aatcaattga aagcttgtta acaatttctc aaaaacccgc attagatcaa
     7501 gtaattggct ctaaaggaag tttgaaagaa gcaattgatc aaattaaaac ggcagtctta
     7561 tatcctggaa aagggttgcc tttattattg atcggcgcta gtggatcagg gaaaaccttt
     7621 ctagcgaata aaatatatga atatgcagta gaagaaaagg tcattcacac atcggcacct
     7681 ttcatagatt ataattgtgc acaatatgtg agcaatccag agttactatc ctctgcttta
     7741 tttggctaca cgaaaggagc atttaccgga gcaagccagg agcatactgg tttgctagaa
     7801 aaagccgatg gaggagtatt gtttttagat gaagtgcatc gcttatctga agaagggcaa
     7861 gagaaattat ttacatttat ggatacgggc gaattctctc ctatggggga taatagtatt
     7921 cggaaaaaag ccgatgttcg attagtcttt gcaacaacag aaaatatata tacgactttc
     7981 ttaccaactt ttcttaggag attgccagtt attgttaatc tacctagatt tcaacaacgt
     8041 ccgtcgtttg aacgtctgtc attgattgat gaattttttg tttctgagag taaaatttta
     8101 gctaaggaac tatctgtttc tgatgcattg attcatttct taatgaatag tgattttgaa
     8161 ggtaacgtgg ggaatattaa aaatattatc aagtatgctt gcggaaacgc atatatccac
     8221 cagaagagtc agcaaacaat tcaagttaaa ttattggata tgcctttaga gtacagttat
     8281 aaattcaagg agcaactaaa taaacccaga aagaaaagag cagatcgaat ctactatcca
     8341 gaagaaacac gacaagtgca actagaaaga aacaatcagc tttttgttca attttttgac
     8401 caattactta ctggattctc tgaagtgatt gaaaataaca aggatattaa actattttta
     8461 gaagaaatgg ttaccaaggt aacacaagta atggatacgc ttatttttca agaggattac
     8521 gaaaaagagc agtctttata cgccattcta aactatcata ttcgtcaaag tattgatttt
     8581 atgcgagaaa gctacgggtt tgaacaagat ggtaaccggg tagtcgcttt ggccaactat
     8641 ctatatgtca aggataatca agtgattttt gaaaatagtc ttcaagaaaa gaaaaccaaa
     8701 gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta tgcaaaaaaa
     8761 atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga cattttcttt
     8821 attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat taagagtatt
     8881 gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa ccgtatgctg
     8941 aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga gaaaattgaa
     9001 gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat tttattggta
     9061 gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa tggaccactg
     9121 ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca agtattaaac
     9181 aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt caaaaagcag
     9241 ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta tacagggatt
     9301 ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc agttagagaa
     9361 ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt tgagactcct
     9421 tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat taatcagatt
     9481 ccatatattg gattagatcg cctgattaat ggggaagcaa ttggcgaatt tgttcattac
     9541 ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat tttcaatttt
     9601 tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt gctatctttt
     9661 gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc taataatcag
     9721 ctgtttttgt tgtaccttca ctgtagttgt atgattgaac gaattgtacg taaagaaagt
     9781 gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat aatggataaa
     9841 attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc agatttagag
     9901 ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac aatgggagta
     9961 atttttgata tggatggggt actcatagac agcgagacat tttattttga gagaagaatg
    10021 aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga ttatgtgggg
    10081 aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt aagagaggaa
    10141 ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc taaagattta
    10201 cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc attggcactg
    10261 gcatcttctt ctccagttgt tgagatagag agtatgctca ttcaatgtga acttactgaa
    10321 tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca tcccgaaata
    10381 tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga ggattcaacg
    10441 attggtatta aatcagcaaa ggcagcaaat atctatacgg tggcactaaa acaaccaatg
    10501 caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact ggaacaaatt
    10561 gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg ctagctattt
    10621 tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg taacaccttt
    10681 tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta ttttgctcgc
    10741 aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca acattctatc
    10801 tatttcagaa cgtaaccttc cccagttcat tgggattcat tagcatacaa ccaacatttc
    10861 aaaaagaaat tgcactacac cattattagc tattttgact aaccccaaaa aacttgctat
    10921 aattcaggtg taggtaaaag gtgctggtat caccttttac gaaaatgctt cggcagtggt
    10981 aatgagtgtg gcttaaagga ctagtccttt aagcttttta ctttgttcgc aatctctaag
    11041 atcaagttaa tgatcgataa gattgctaga atattttgca acattctatt tcagaacgta
    11101 acctttccca ttccactgtg attcattggc aaccctcctc acaaccaacg tttcaaaaga
    11161 aattgtaaag ttgttggggt accacgaccg aacaatacta cacctgaatg atagtaagtt
    11221 gtctcattaa gtatagcata tcctcttttt gggtaaatag aaatcggtta cattttttat
    11281 ccaagaccaa gttttttcat ttggtcttat ttgctatacc caaacaattc cgcagtatag
    11341 tcgacggtat tgattttatc aggtgagtaa tagaaacttg ttttccttcc ccaaaacatt
    11401 acctataaag gagaaaaaat ggtatcgtaa ttatataggt ataggatgaa atttttttct
    11461 aagaggaggg ttgttgatga aaaatttaca gttaaaattt attgttgatc catcgattgt
    11521 acgctggttt cggattatca atcaatttga gcgcaatcaa cttgccacaa aaggagaatt
    11581 agcaaagagt aatcatgtat ctgaacgaac cattcaaaca gatgtcaata aaatgaagga
    11641 ctacttttct gaaagtgttc aatttgtttc aacgaaaagt gggtattctt ttcagaagga
    11701 aagaccggtt ctttttctca agcagaagga gcaactactg gagaacgaaa ttttatttga
    11761 gttgttaggg aatgtctttt atggcgaatt ggaagatatg acggagctaa ttgatcgttt
    11821 tcatttatct gaagcgactt ttcgtcggta tttgaagcag attcaaccag ttttggaaga
    11881 atacggctta gaattgagtc tttatcctct tgatttaaaa ggagaagagg caaatattcg
    11941 caagtttttt aaagacttct attatgaggg ggagatgacg ccccatactc ttgttccacc
    12001 aagggatctt catgaacttg tgcaagctac attctatgat aaatttgagt atttctcgat
    12061 tggaacaggg acttctcctg ctgaatttta ttatagtttg tatattgcga ttgagcgtgt
    12121 gagacaagga aaaacgatag ccattcctca aggtctgatt gagcgagtaa ttgctagtga
    12181 gaattttcgc ttaatgtatt ccttgaaaga agcgattcaa acaaattatc aagttgcttt
    12241 atcagaagag gagttttgtt ggttgtatt
//
LOCUS       DAJSVF010000075        12242 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSVF010000075 DAJSVF010000000
VERSION     DAJSVF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.078"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(131..688)
                     /gene="frr"
                     /locus_tag="ON550_002112"
     CDS             complement(131..688)
                     /gene="frr"
                     /locus_tag="ON550_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR3955185.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(691..1413)
                     /locus_tag="ON550_002113"
     CDS             complement(691..1413)
                     /locus_tag="ON550_002113"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR3955186.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1549..2430)
                     /locus_tag="ON550_002114"
     CDS             complement(1549..2430)
                     /locus_tag="ON550_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR3955187.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2529..3311)
                     /gene="rpsB"
                     /locus_tag="ON550_002115"
     CDS             complement(2529..3311)
                     /gene="rpsB"
                     /locus_tag="ON550_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR3955188.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3670..4149
                     /locus_tag="ON550_002116"
     CDS             3670..4149
                     /locus_tag="ON550_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR3955189.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4366..5457
                     /gene="phnW"
                     /locus_tag="ON550_002117"
     CDS             4366..5457
                     /gene="phnW"
                     /locus_tag="ON550_002117"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR3955190.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5450..5578
                     /locus_tag="ON550_002118"
     CDS             5450..5578
                     /locus_tag="ON550_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3955191.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5582..6127
                     /locus_tag="ON550_002119"
     CDS             5582..6127
                     /locus_tag="ON550_002119"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3955192.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6581..8272)
                     /locus_tag="ON550_002120"
     CDS             complement(6581..8272)
                     /locus_tag="ON550_002120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR3955193.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8692..9639)
                     /gene="arcC"
                     /locus_tag="ON550_002121"
     CDS             complement(8692..9639)
                     /gene="arcC"
                     /locus_tag="ON550_002121"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR3955194.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9754..10773)
                     /gene="argF"
                     /locus_tag="ON550_002122"
     CDS             complement(9754..10773)
                     /gene="argF"
                     /locus_tag="ON550_002122"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3955195.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10864..12093)
                     /gene="arcA"
                     /locus_tag="ON550_002123"
     CDS             complement(10864..12093)
                     /gene="arcA"
                     /locus_tag="ON550_002123"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR3955196.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3356 a         2532 c         2207 g         4147 t
ORIGIN      
        1 tttcctatga aatcattgtt tttttattta aaaactgaat aattttttct ttttttaatt
       61 tttaaataaa agaaatataa ataaaaacag aggaagcgga atgatccgcc cctctgtttt
      121 tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc ttatctactt
      181 ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc aaatcatctt
      241 cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca cgacggatgt
      301 tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct aattcttttc
      361 tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac
      421 tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa
      481 aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg
      541 gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc
      601 ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt
      661 ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt
      721 ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg
      781 aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc
      841 aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc
      901 agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc
      961 tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc
     1021 aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc
     1081 tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa
     1141 cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg
     1201 agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccgcccac
     1261 aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt
     1321 gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc cactcaattt
     1381 taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc
     1441 cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa
     1501 aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac
     1561 ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc
     1621 gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat
     1681 atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa
     1741 gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc
     1801 tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata
     1861 acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt
     1921 tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc
     1981 agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac
     2041 ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga ttttcattgc
     2101 agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc
     2161 ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc
     2221 agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt
     2281 cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc
     2341 tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc
     2401 ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt
     2461 aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc
     2521 attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt
     2581 gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt
     2641 ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg
     2701 tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct
     2761 acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca
     2821 acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca
     2881 agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt
     2941 ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc
     3001 cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt
     3061 gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg
     3121 ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt
     3181 tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga
     3241 ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa
     3301 attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc
     3361 ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat
     3421 gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac
     3481 ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta
     3541 gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg
     3601 aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg
     3661 agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc
     3721 ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg
     3781 caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat
     3841 ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa
     3901 gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt
     3961 ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag
     4021 atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg
     4081 gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca
     4141 cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt
     4201 ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt attcaaattt
     4261 ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc
     4321 ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta
     4381 acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt
     4441 acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt
     4501 gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag ctccttcggg
     4561 gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac
     4621 ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt
     4681 tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag
     4741 aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat
     4801 aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat agtggacgcg
     4861 atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga ttttttgatt
     4921 tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa
     4981 gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct atatgaccag
     5041 taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt
     5101 ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat
     5161 tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat ggggttccag
     5221 cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg
     5281 cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt gatctatccc
     5341 gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa
     5401 gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac
     5461 tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc
     5521 ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa tccgctaacc
     5581 gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa
     5641 acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca
     5701 acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac
     5761 attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca cgaatggatt
     5821 taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt attcaccaga
     5881 cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca
     5941 taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac
     6001 ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg
     6061 tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaagat
     6121 cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat ttgtgtaaac
     6181 aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga atgactttta
     6241 tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac agccaagcaa
     6301 aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
     6361 caccgtttat tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat
     6421 ttgcaaacaa acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac
     6481 tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct
     6541 aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat
     6601 gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta
     6661 agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga
     6721 atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag
     6781 cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt
     6841 cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga
     6901 ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt
     6961 cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg
     7021 ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc
     7081 tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct
     7141 ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggt
     7201 gcatgtcgtc agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat
     7261 gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac
     7321 ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa
     7381 tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt
     7441 tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag
     7501 cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt
     7561 tcatcgattc atcacggaac cattgccata attgaatagc ctctttatcg ccttcttcca
     7621 gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat
     7681 ggaattggac gtataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg
     7741 taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat
     7801 ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga
     7861 taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt
     7921 cgattggtac tgttccttga ttcccgatgt tgctatcccc ataatgttct ttttctttca
     7981 ctacggtttg caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag
     8041 gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag
     8101 cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat
     8161 ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc agtgttaaat
     8221 catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc
     8281 ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt
     8341 atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc
     8401 agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt
     8461 cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca
     8521 agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa
     8581 aaaaacgaaa ggctagtcgt ttcataaaaa aaaaatccag atggcctaaa ggagaagaaa
     8641 taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa attaatcagc
     8701 tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta aagaagtgat
     8761 gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta ctttcggaag
     8821 catgcttcct ggagcgaatt gcttttcatc aatgtattgt ttcatttctg aaacagtaac
     8881 agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt ctactcctgt
     8941 caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag caaaatcttt
     9001 atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag gaatcccacc
     9061 gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga cacgtgcttc
     9121 tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc cagcatcttc
     9181 tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt ccgtgtagaa
     9241 tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca caataacttg
     9301 agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt cgcccatggc
     9361 gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat ctaaaggcat
     9421 tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac cgacttgcgg
     9481 accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca ctaaatactt
     9541 tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag acaagatggc
     9601 attgccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc tttcttcagt
     9661 tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc ctcaatgctg
     9721 actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa acaagttacc
     9781 taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt ggtcaaattg
     9841 acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga tgccaaagcg
     9901 ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca agcagtgcaa
     9961 gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga tttggtaagg
    10021 tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata cccatacatc
    10081 agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa tcatcaattg
    10141 tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt caggttgtaa
    10201 ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga ctaataaaga
    10261 gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt ctactgttcc
    10321 aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc attcatcagt
    10381 caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca ttttttgact
    10441 gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg tatcttctgt
    10501 ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat gtgcacctag
    10561 atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt caaataaaag
    10621 cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca agtctttcaa
    10681 gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt tttctgctaa
    10741 taagcttctt ccttggaata ctgattcttt catattaaac attctccttt agattgtttt
    10801 tatttaagat taaagaggaa atcccgacgg cagctgacag taagactgcc gtcaggaaac
    10861 aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac caccacgacc
    10921 tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac gaagcaattc
    10981 gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg ccaatgtgtt
    11041 tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac cagttgggat
    11101 cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt tatctgaatg
    11161 agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct taccgtcttt
    11221 actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa atacggtatc
    11281 caaatgcatc attgcacggt tgttcggaat tttgattgcc aacacttttt caaatcctga
    11341 attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag ttcgttgaga
    11401 aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt cgatatgatc
    11461 tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg gatgatgttt
    11521 caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca tcttattgac
    11581 tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt ttggcattgg
    11641 gtccatgtaa aatggatagt catcgtctgc tgacagatca tatagactgc tagaacgtac
    11701 ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt gcgtttccat
    11761 tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat gagattcatc
    11821 caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt ctgccgctaa
    11881 tttttccaag taaagagtct cgacgccttc attttgcaat gtcttcgcaa agttgtcatg
    11941 ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat ccataatatc
    12001 tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt aaaactgtct ttaatttccc
    12061 tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc tttttgatta
    12121 catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat tcaaaatgag
    12181 acattcctca cattaatatt cgataattaa ataattaaac gtttttctat ttttaagcgt
    12241 aa
//
LOCUS       DAJSVF010000076        11712 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSVF010000076 DAJSVF010000000
VERSION     DAJSVF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.079"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(175..582)
                     /locus_tag="ON550_002124"
     CDS             complement(175..582)
                     /locus_tag="ON550_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3955197.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(577..1781)
                     /locus_tag="ON550_002125"
                     /pseudo
     CDS             complement(577..1781)
                     /locus_tag="ON550_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1907..2125
                     /locus_tag="ON550_002126"
     CDS             1907..2125
                     /locus_tag="ON550_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955198.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2146..2781
                     /locus_tag="ON550_002127"
     CDS             2146..2781
                     /locus_tag="ON550_002127"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3955199.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2974..3213)
                     /locus_tag="ON550_002128"
     CDS             complement(2974..3213)
                     /locus_tag="ON550_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955200.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3237..4760)
                     /locus_tag="ON550_002129"
     CDS             complement(3237..4760)
                     /locus_tag="ON550_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3955201.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4778..4900)
                     /locus_tag="ON550_002130"
     CDS             complement(4778..4900)
                     /locus_tag="ON550_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3955202.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4930..5913)
                     /locus_tag="ON550_002131"
     CDS             complement(4930..5913)
                     /locus_tag="ON550_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3955203.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5937..6086)
                     /gene="rpmG"
                     /locus_tag="ON550_002132"
     CDS             complement(5937..6086)
                     /gene="rpmG"
                     /locus_tag="ON550_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3955204.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6107..6484)
                     /locus_tag="ON550_002133"
     CDS             complement(6107..6484)
                     /locus_tag="ON550_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955205.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6516..6695)
                     /gene="rpmF"
                     /locus_tag="ON550_002134"
     CDS             complement(6516..6695)
                     /gene="rpmF"
                     /locus_tag="ON550_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3955206.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6710..6979)
                     /gene="rpsN"
                     /locus_tag="ON550_002135"
     CDS             complement(6710..6979)
                     /gene="rpsN"
                     /locus_tag="ON550_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3955207.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7502..9268)
                     /locus_tag="ON550_002136"
     CDS             complement(7502..9268)
                     /locus_tag="ON550_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955208.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9228..10982)
                     /locus_tag="ON550_002137"
     CDS             complement(9228..10982)
                     /locus_tag="ON550_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955209.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11092..11661)
                     /locus_tag="ON550_002138"
     CDS             complement(11092..11661)
                     /locus_tag="ON550_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR3955210.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3692 a         2260 c         1692 g         4068 t
ORIGIN      
        1 aagtaatcta accgactgtt aactaaacca ttgcaggtcc cgttaaagat acttgtgcct
       61 gttgtcaccg cttctttttc ttgtccacaa gcgcttaaca cacttaataa agcccctgct
      121 attattaata ataatatctt tttcttatta aaaacctcct catctatttc cagattagta
      181 aatgtagtaa tctttgcttt cttttttctc aatactaata actaacttat ggggtaaaca
      241 aatactcgtt tgaccgggtt ttgagatcca tccagttcta accgcgattt gatcaggact
      301 attatcttct ttaactcgaa ttcttccatc tttaatctca acaatattat attgtccctt
      361 cgcaggatga taattcacga gtttatgatt gatcttatcc aaatcgaagc gatccatttc
      421 cttgccatca atttgaacga tggcatattt gatagcatct tcagttgttt cattctcctt
      481 ttctatagag aatacaaata agggaataaa agagggaata atcaaaatgc caataattat
      541 gacatcgcct atcttgacac cagcttttct taaaaatttc attaacgata tctggctaaa
      601 catattagaa tttttccatg gaaatggtga aatttttcgg aaattatctg cattaagaga
      661 atcagttaaa caaccctcaa gttgttcctt aatacattgt tgatccatct tcataccaat
      721 aaaaactagt ttattaatgc gttcaccaaa caacggatgc caggcttcct tcaattcggg
      781 tttttttgta taatcatttg acactcaatt ttaggtaaac tagctaccca ataatttaat
      841 gattcaatac ttttatggct acccgatgtt tcaaagatcg tcgaaaaatc actatgttgg
      901 gcaaaccaaa gaaacccttt tgctcttgta atattgccag gaaaattttt atccgtaaga
      961 tgggcaaatt tttgtgcgtc aaggagtaac cgatttcgat agataaaact tgagatacca
     1021 tattcttctg tttcaggaat atgattttga tagccaagct ctaactcttt gaaccagccc
     1081 attgaatagg aagtctcatc tatattaaaa agatttgtat ttagaatttt cgataagtct
     1141 acttcacctt taattgtcct aattattttg gcattggact gtaacttagc aattagaccg
     1201 ttaattttgt aaatcgtctt ggttgacact aagtcactat tatttaaaag taatatgtta
     1261 caacactcga attgatcaat taacaagcca cgaatatcct gttcttcttc aactacatta
     1321 ccttcagctt ttctttcctg aatcttttca cccgtttcga aattatccca aaaacgtttt
     1381 acatccacta ctataataat ggcaccaatc tctaatcgtt gggtcaaatc aatgtctaaa
     1441 gggtttttag ctaatataat tgcttgaata attggaattg gtttgctgat tcctgaagct
     1501 tcaataacaa tttgattcaa gtcagaaaat tgcactaatt gatctagttc aacaatcaaa
     1561 tcttcgcgta atgtacaaca aatgcatcca ttcggcatcg aaacaagttt ttctccagtt
     1621 cttttaaaac ctttcttgtc gactaatacc ccatcaacat ttacttcccc tatgtcattt
     1681 acaatgacag caatttcctt tccgtgatca ccgtacagaa tatgatttag aagtatcgtt
     1741 tttcctgaac caagatagcc agttaagatt gtaataggta ttttttcata gggaacctct
     1801 ttctatacct tgaatttaca ctttaagtgc aacacataaa aatgagtcat aatcgatatc
     1861 caactagtat aagtttgtcg agaacactcc tagaaggaaa ataattatga gccagtattg
     1921 tcatcttact atactcaaac gagaaagaat ctttctgctt cacgaagaag gattttctat
     1981 tagtataatt ggcaacgaca ttcagcatag cccgtttact attttacatg aactgagaag
     2041 aaacaagaaa aaaactaatc accttccaaa gcggaaaaaa attatgctta tcgaagaaaa
     2101 aaatgcggac gttatcacat tctaaatgat ccgtgagttt ggtcaattgt tcgtcattta
     2161 tttgtcgatt ttcagtggtc gccagagaaa atctcgaatc gtttgaagca tgaacatatg
     2221 gacttttcaa ttagttttat cacgatctac cgtgcaattt gcaatgatct tttcgataca
     2281 gaacccctct cacacggcaa taaaggcctg atccgttcat tgcaccatcg aggaaaaaca
     2341 agacatacaa aaaatcatgt cgagtctcgt gggaagattt gccttactca ttcgatccat
     2401 ggccatccaa aatccgcaga tgatagaagt gattttggtc attggaaagc cgatattgta
     2461 gctggcaaaa ctagtggtac ttgtttagtg actttgacag acagaaaatc atgcttcctt
     2521 ttagcaggaa agaaggtccc cagaaagaaa gccgcttatg tgagcgagaa aatgattgaa
     2581 cttttaagca gtattccaaa tgaggcggtc aaaacgatca ctccagatag aggaaaagaa
     2641 ttctccaatc atttcgcagt tacagaagta ttgaataata tgccctttta ttttccagat
     2701 cctcatgccc ttggcaacaa tgaacaaatt aaaatacgaa tagactcatt cgagaatact
     2761 tgccaaaatc gaaagagata gctacggtag ataactcaac gataaatcag atgattgaaa
     2821 aactgaataa cagacctaga aagtgtttag attggagaac accttatgaa gtcttctttg
     2881 ataaagtgtt gcgcttgatt tgacaattca agaaataata aaaagcagat caaatccaaa
     2941 taagggaaaa atgatctact ctggtattat caattaattg gccaagctat tatgccgttt
     3001 tgctaaacgt ctacttcttc cagtattgac cttgataaaa caacaatcag gatcccgttt
     3061 taatactcta tgaactgtgt caatactaac atgtaaagtt gcagcaatat cagctaatgt
     3121 actcccattt aatctcattt gctttgcaaa ttgataacgc cgataagcat caatattctt
     3181 ggaatccata gacaataata cttttcttac catgtatttc ctccaaagat tggattttaa
     3241 tgggccatca tttcttgagc aatctcatgc tcagataatg aagctggata gaaagtaggc
     3301 caattatgca tttcattata aagtttttct tgactttctc caccaaagaa aatatggaaa
     3361 tgctctgcct tacttggtgc aattccatga tcgctaaatt gaatatattt aaaacgacca
     3421 gcacctgcat catcagtttc aaaattaaaa cgaacaccac ggtttccttt ttcataattc
     3481 aaaattttgt atccaacata tttgtatgtg tattgatgat gttcaccgtt gactacaaaa
     3541 tcaatggtgt tgtctttaat gttgatagta tcaatatctg tcttatagcc agtagtataa
     3601 taatctttgt actcggcagc tgtcatgtct ttattgagtt ttgctttgta atcgaaaact
     3661 tcatccaaaa ttccatcttt taacaacgga taaacagatt gccattcgcc tgtataatca
     3721 ctcaattcgc gatccttcac atcggcatct ttaaagtacc cattagcaac tgtcttttca
     3781 tccttcgctt ctttttctgg aactacctct tcaccagcaa ccatagttgt tttctctaac
     3841 gcttttagat tgtcttccat tactgaaaca taatcttcac cattatccat ttgttctttt
     3901 gtaagacttt caagaggatt caatacttcc aattttatcc cagcttcatt ggccaaagtt
     3961 tttgcgattt tatcattagc attcttttca aagtaaatat aattgatctt atttttttta
     4021 acatactctt ttaattcagc taatcgacca gaagatggct cttcttcagg acttagtcca
     4081 gcaataggaa cttgtatcaa tccataatcc aaagctaaat atccaaaggc tgcatgttgt
     4141 gtcacaaaac ttttttgttt cgcttcttta agactagtgg tatagtcagc atccaaccgt
     4201 ttcagtttag tcaaatactt ttcagcattt gtctcaaata cttttgcttt tttcggatac
     4261 aatttaacta actgatcctt gatatttgaa acttccttaa ttgccatttt gggtgaaacc
     4321 cacgtatgag gatctagttc atgatggtga tcttcttctc cgtgatcatg atcttcttct
     4381 tcactacctg gcaataaaat catgtctttt gttccttcta ctacattagg cccagctttc
     4441 ttccaacttt ctttagcttt tggtacccat gattccatgt tttcgttatg atatacaaaa
     4501 acatcagcat catgagcttt tgccatatcc ttagctgagg gctcatagtc atgaggttct
     4561 gatcccgctg gaaccattaa atccacgttc gcttcatcgc cgacgatgtt tttggtgaaa
     4621 tcatacattg gatagaatgt tgttacaatt tctaattttc catctttatc attcgatact
     4681 tttgcatcat tagtgctgga gcaagctccc aataatccta gagctgctat cccaataagt
     4741 cctacactga ttttcttcat tctttttcct tccttaatta tgctaatttc ttcattttat
     4801 tttgtttatg ttctttgata atttttaatg ccctttttct agttttgata ttcgggcttt
     4861 taagtcgtcg atatgcacta gatatatccg ttttctccat tagaaattac ccctaatcaa
     4921 tacttatcat tatttgcttt ccgccagtaa tttatcaaaa ctctgtttga tcctttcttc
     4981 atccaacatt tttccaatta gaataatagt ggtttggtct ttttgtcgag tggtgtattg
     5041 aaaattaacc tgttcgttta ccccttgtat ggcaactata aaatcttgat cttgcaaagc
     5101 aatcaatcct ttaaaacgat acaggctccc ttggttggtt aaaatcagcc ggtcaattaa
     5161 ttggaataaa aaatcttttt ttatgtcttg atctgaagtt aagagaattg atttaaactc
     5221 atgttcttga tgatgttgat gatagtgttt gccattatag tgattttctg atgaatcact
     5281 ttcaaaaact aagggatgat tgaatttttc caaattataa aaatcctttt tttgaatcgt
     5341 ttcactatgt gtgaacattc gaatatcgga taaagggtta atacttttga tttctctctg
     5401 taattttttt agtgcagata aattcaagtt ttgttttcta gacacaaaaa tgcgatctgc
     5461 tatgacaagt tgcttaaggg cttgtggttc tctttcaata agttcaaaac tgtcactatc
     5521 aataacagtc aataaactat cgatatatac tcttcctctt agttgaggcg ctgttaaaag
     5581 tgtttgaatg attggttggg gatctgctat acctgtagtt tcaatgatga tttgtgaaat
     5641 tggttgttga tgttcttcta ttgtttccag taaagaaatc aatattgcta ataaatcact
     5701 gcgaagactg caacacatac atccattatt tagttgtaac acccgttcct cgctatgaac
     5761 taataatttg tgatcaatcc caacttcacc aaactcattt tcaattataa taatttcttc
     5821 cctggggtag ggtgagactt gcaaggcttg attgattagc gttgtttttc cagcccctaa
     5881 aaacccagaa acgattgtaa ctggtatgcc cattttgtca cctttttacc acttagctat
     5941 ttcacttctt tgaaaattgc ccgtctgcgc aattttggcg aatacttttt taattctaac
     6001 cgttcagggt tattccgttt atttttgctg gtgagataca gacgttctcc agtttcgaca
     6061 cattccaaaa taatattttg acgcataaat cttcctcctt caatacttat cgatcttcaa
     6121 gatatgcttg aatttgttgt gcatcttcaa aagttaactc gccagattcc aattgtgaaa
     6181 ctaaattttc tttcatatca acaggtatct cccaataacc atcaatacat tgaaataact
     6241 cctcgtcata ctcttcaaaa attggataga ttcttaaaaa atcatcaaat gataagcctt
     6301 ggtcctcata gtaatctttg actaatccat tttcaattgc ctgttgggtc ggtaaacccg
     6361 tttcagtaat aattcctctc tctaccatcg aacgataaaa agtgcattta tctagcgatt
     6421 gctgggtcgt ttcaagataa tactttaatc cttcttcaac taaaggtggg taaataattt
     6481 ccattttgat tccctcctaa aagttatata ttaaactatt ttgaagctcc tttaattact
     6541 ttctttccat tatagtaccc ttttaaagat acatgatggc tacgtctata atcaccattc
     6601 gcttcgtcaa atgaaatttc tggttttact acagtttgat gagttcttct taaccgtttc
     6661 ttcgccttag atgtttttct tgctggtact gccatgatat ttcctccttt taccaacttg
     6721 ctttttttac tccaggaatc aacccttgat gggcaagttc tctaaactta atacgggaca
     6781 taccgaactt tcgcatatac cctcttggac gaccatccgt ctgatcacga agcctcagac
     6841 gattcggatt tgaatctctt ggcaatttag cgagagcggt gcgatctcct gcggctttta
     6901 attcctgacg tttatctgcg tacttttcaa tcatcgccat ttgtttcttt gctttagcaa
     6961 ttttcgactt ttttgccata tacctttata ctccttttat tttgatttag acatctcaaa
     7021 tcgtaatcgt tacgttttga cactgatact ttaccaacaa gagttgctct tgtcaacaat
     7081 aaataacatg aaataaaact catatgaata gataactatt tgaaaacgaa tttatcggta
     7141 catagagata atcaaatcat ttaaaagaat atgcagttta agctctgaaa aataagaatt
     7201 cacttttatt agtccaatca aacacaaata ccgttttaag tagcttttcc tctacttaaa
     7261 acggcaatat gaaagaaact ataacttttt attcaatctt ttatgtttaa ctggtttatc
     7321 gccaattttt taaaactttg attattcctt atattataag aatagacatt aagtgaacta
     7381 attcaatcat actgattctt tcgtgtattc aaaattagat gctgtttaat aaaattattc
     7441 tttttgaaaa agaattataa ctatatccca aaaattgtag gttaatttaa agcctatttc
     7501 atcatcgtga ttgagcttct ttttcataga cgaatttctc agatagttta gttttcttta
     7561 tgagattttt gtatatttct gaccttcgat acaactcact atgagtacct tgatcaacta
     7621 tttttccttg atccatcact acaattttat ttaccttttc aatagacttc aagcgatgcg
     7681 atataattag tactgtctta tcttgtatta aacgatttaa actttcctga atacgttttt
     7741 cattttccgc atctaaactg gcagtaattt catctaaaat taaaattggc gcatttttca
     7801 aaaaagcccg tgctatggat aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt
     7861 ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg cagttagcta
     7921 aacgagctgc ttttttcact tcctcatctg acgcttgact atttccaata cgtatatttt
     7981 ccaaaatact tgtattaaac aaagttacct cttgaaaaac aatagcgatt ttgctaaaca
     8041 aagaagctgg agatacacgt ttaatatcat acccatcaat taaaatacag ccttcatcat
     8101 aatcatacag tctagacact aatttcaaga tactagtttt cccggaacca ctagcaccta
     8161 ctaacgcagt cacttctcct tgtttagccg taaaagagat atgatctaaa attggggtat
     8221 tgttatcata tgagaaagag acatctcgca actcaatgtc aaaagatttc agaggactat
     8281 ctgatccctc ttggatactg gtttccttca tagctctaat tcgctgtatt ttaggagcta
     8341 aataaaatat ttcaagcact gcttctttca tagaatcgaa ggaatccttt atttttattg
     8401 cggctagtaa gtaaccaacg acgtagagta tagtcacttc acccgttaac agtaagtgaa
     8461 cacctactag aatgactact gctaaactaa catatgagaa aatagaggat aaggccatga
     8521 ctgaaaaagt acttaattct acctttaaat gaatcctttc actctcttcc atttttttga
     8581 aaagtctgtc ttgaactttt ttagatagat taaagctatt aatctcttgc tgcaattcta
     8641 tagtttcttg aaattcttcc gagttttctc tcaaagtatc gtaatattta gtattggctt
     8701 tcgtttggga tttctttgat aacaagatta agacaaaact aaataacgtt ggcaaaataa
     8761 cagctaatcc cattttgaca ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca
     8821 gagccatacc gccggcttta ggtattgcat gactcatcgc atgctcaata ccttcaacgt
     8881 cagacatgat agtttgagat aaatctgata aattatgttt tgaaaaatag gataatggta
     8941 aatttgacaa atcgtctgca atttgcattc ttaaatgcgc actttcttta taagtactat
     9001 tatataagtt ttcatactcc ctatctaaca accaatacat gaaaagcaaa gtaacgaacg
     9061 atactgtaaa ataaagccaa ggcggcttta cattttctaa aactaattcg tctatcagca
     9121 tcattataat gaatgctggt accatattaa tgcagtatac aaaaaagcta gaaatagatg
     9181 ctttaaccaa atcttctgcg ccctgattag ttaaagcgaa tttgttttta taaaaacttt
     9241 tcattgtcaa gcctccaatc atttgcattc tcataattag tgaccaattg tttatacact
     9301 tgatgtttag ccattaattc attatgattt ccacgttcta ctattttccc ttctgacaag
     9361 acaagaattt catctacaga tttaatgctg gataatcgat gagctatcat aataactgtt
     9421 ttttctttca tcagatgttg aaaagccttt tgtagttcat actcattatc tggatcaata
     9481 gatgcactgg cttcatccat aataacaatc tttgcatctt ttaaaattgc tctggcaata
     9541 gctatacgtt gtttttctcc tcctgacaga taaactcctt tactaccaat taaggtatgc
     9601 tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat
     9661 actttacttg ctggcgcttg aggatttgct atagaaacgt tatcgtaaat actctgattg
     9721 aatagtttcg tatcttgaaa gacaaaagca attgtgttaa taattgtgtc ttttgaatag
     9781 tgatttaaag gcttaccacc aattttaatc tgtcccttat ccacattgta gaaacctgaa
     9841 attagtttag ctatcgtact ctttccgctt cctgattttc ctactaatgc ataaattctt
     9901 ttttgttcta aaactaagtt gaaattttct aaaacacact cttctttgta tgaaaagctg
     9961 acattttcaa atgcaatgtc acaattttta atctgtgtct cgttcccata ttttagttgg
    10021 tcttgcttca tttctaaata aagggagtct agtgtttcga ctgcatatct tgcttcaaac
    10081 aagtgcatac ttacatacat tattctcatg aaagaaacaa aaataattcc acttaaaaaa
    10141 aacaacatca acaagtcaac agctaatatc ttgggagtgg taagagaagt tataaaaaag
    10201 gttataggaa ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa
    10261 ggtgttttcc cactcaaaga atacttatat gcaaacttag cataatcgtt aattgcttta
    10321 ttcaaggctt taaatgatgt tactttagca ccaaatatct ttataacttg aattcctcgg
    10381 atatattcta ccgtttcaga gcttaacaca tctaacgatt cttgatattt ttgaataaaa
    10441 tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat ggcgattaga
    10501 accaatccaa ttttaaaatt aatgaaaaag cctaaggcaa ctgataaaac aggtgttact
    10561 atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt agccgcgttg
    10621 tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg aaaacttgct
    10681 tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa cccaaggata
    10741 tgagaaaata agccagaaaa aatataaaat aaagctccta aagttaacca agcaacagtt
    10801 tgaaccgcat aactcttagc taaatattca tctcctacaa taattagaga cttaagaaat
    10861 ttgtaaatac tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg
    10921 gctaaccatc cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc
    10981 attttttcct cccccgcatt caatttcttc tcttattcgt ttacaactgt taatgctttc
    11041 cgtaatagcg taaattagag atgtacaaac agatagaagg taaactacgt tctatttttt
    11101 gtattttttt aagacttttc ttccaatcaa tacaccaatc atagataaac ttgctacaac
    11161 taacactgaa atgattcccc atatacctac cgcatagtgg atcattgcat ccattgttgc
    11221 agcagattgt cctttatcta tccaatcttg acgaaaacga tctgtaaaga accatatagg
    11281 aatccactgt ccggcagcac tgcctaattg cattagagcc caagacgcaa cttgaggaat
    11341 tgctcgatca tatccaaata cagatagaat aatatcagct aagatcgcca tactaatcga
    11401 actgataaac caaggtaagt acgccccgcc cattaacgta aaaaataaca ataaaacaat
    11461 tgtgtaaata gtaaaaattc cttttctagg gtagttatag ctaatataaa caaatatgat
    11521 tccagataat aatccaaaaa tccctgggga tatgctgtga ccaaacgccc caccaatact
    11581 actaaccatt attgaaaaaa agtaaattac tagggaaata actgtagcta aaccaataaa
    11641 aatataatct tttgttttca tatatattct cctcaattct atttcgttat gtcattaaaa
    11701 atctgttcta tc
//
LOCUS       DAJSVF010000077        11685 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSVF010000077 DAJSVF010000000
VERSION     DAJSVF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.080"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..1497)
                     /locus_tag="ON550_002139"
     CDS             complement(<1..1497)
                     /locus_tag="ON550_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR3955211.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(1513..2037)
                     /locus_tag="ON550_002140"
     CDS             complement(1513..2037)
                     /locus_tag="ON550_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR3955212.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            2335..3084
                     /locus_tag="ON550_002141"
     CDS             2335..3084
                     /locus_tag="ON550_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3955213.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            3075..3860
                     /locus_tag="ON550_002142"
     CDS             3075..3860
                     /locus_tag="ON550_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR3955214.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            3829..4545
                     /locus_tag="ON550_002143"
     CDS             3829..4545
                     /locus_tag="ON550_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3955215.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(4647..5681)
                     /locus_tag="ON550_002144"
     CDS             complement(4647..5681)
                     /locus_tag="ON550_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR3955216.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(5685..6743)
                     /locus_tag="ON550_002145"
     CDS             complement(5685..6743)
                     /locus_tag="ON550_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR3955217.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(6964..8112)
                     /gene="nagA"
                     /locus_tag="ON550_002146"
     CDS             complement(6964..8112)
                     /gene="nagA"
                     /locus_tag="ON550_002146"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR3955218.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(8188..9024)
                     /locus_tag="ON550_002147"
     CDS             complement(8188..9024)
                     /locus_tag="ON550_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955219.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(9208..10425)
                     /locus_tag="ON550_002148"
     CDS             complement(9208..10425)
                     /locus_tag="ON550_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3955220.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(10574..10753)
                     /locus_tag="ON550_002149"
     CDS             complement(10574..10753)
                     /locus_tag="ON550_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955221.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            10808..10891
                     /locus_tag="ON550_002150"
     tRNA            10808..10891
                     /locus_tag="ON550_002150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10842..10844,aa:Leu,seq:gag)
     gene            complement(11131..>11685)
                     /locus_tag="ON550_002151"
     CDS             complement(11131..>11685)
                     /locus_tag="ON550_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955222.1"
                     /translation="FFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYV
                     NGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKK
                     TSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQ
                     KNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         3524 a         2416 c         1995 g         3750 t
ORIGIN      
        1 ttcttccttc atcgagtttt gttctgcctc ttttggcgct tcatacatat ttacttggct
       61 agtagtccca cgtgtcgaca aattctctac gatttccttc aagtcaagtc ctgttgtcgc
      121 ttttagtgtt tcttgagtag aagaaagcaa atttgtggca taatttgtca ctcggttcgc
      181 tccactggtt tctcctgaac tgcttcctgt atcgacaaca gagatttttt cgatatttcc
      241 tagcggctgt gctgcttcgc gcatcaattg cggtagcata tcgataacca tactcaagac
      301 agctgcttcg ccatattcct tgaaggcatt ggcgatcttt tgttttgctt cggcttctgc
      361 ttttcctttt gctagtgctg cttcggcttc tgcttgtcca gctaaacgtg tcttattggc
      421 ttccgcttct gccagtgctt ccacacggaa tcgttctgct tcggcttcag tcacttcttt
      481 gactttttga gcttgtgctt cttgttcttt agcgtaccga tcggcatctg cttttttctt
      541 cacttccgaa tcatattgct tttcacgacg tgtgatttct ttttcttcca gttcgatttg
      601 tttttgacgt tctacgattt taatctgcat ttcctgttcg attacttgtt gttgtgctcg
      661 ggcactttct aaattgtagg cttggtctgc ttttgcttta gcaacatctt gttcttgttt
      721 gtaggttgct aatttcagtt ctttttcttt taatgattct gcgatctctg tttgtcgctg
      781 taattcagct gcttgtgatt ctttttctgc ttgagctttt ttgatacgcg tttcttttag
      841 agcttccgct tctgctatat ctgcatctct tttgacttgg gcgattcttg gtttccctaa
      901 agagtcaaga taaccgtttt tgtctctgac ctctttgata gtgaaagaaa cgatgatcaa
      961 ccccattttt gcaaggtcta cagaagcgac ttcttgtacg ctttgactga acttatcgcg
     1021 attttgatag atttcttcta cagtcataga accaagaatc gagcgcaagt gtccttctaa
     1081 tacttctctt gcttcatttt ccagttcttc tcttgtcttg cctaaaaact gttctgctgc
     1141 tgtagcaatc tcttctacag atgaaccgat tttgatgatc gaagtaccat cacacatcac
     1201 cggtacgcct tgttccgtat acacttcagg ggtggagacg tccaatttac tcgataatag
     1261 actgatccga ttcgaacgtt gaaaaactgg caagacgaat gcgccacctc cacgcacaat
     1321 tttgattttg ttattgcttt catcaatgtg cacatttttg cttcctaaat aacttccact
     1381 gatgatcaac gcttcatccg gttttgctgt ctggtacttc gctacaaaga cgatcagcaa
     1441 catcagtaaa atcaaagctg cgattgcgat cgcgataaaa acaactggta tttccattta
     1501 acacacgctc ctttaagctt ttatcatgct ttcataaggc atgacataac agattctttc
     1561 cttgatctca atgatcaata ctttcgttcc ccgctcagct gtctgttctt gggggctgta
     1621 aaaacaagct ggacgactaa tggaaccggt aacacttttc aactgaattt ctcccatacc
     1681 tcgtacaggg atcggtgtga tcacaacagc tacttgtcct tccagagttt tttcagaaga
     1741 cgacatcgtt gattcggcat gtttcatcgg catcaatata tagaaattaa gcaaaaatac
     1801 caacactatc gatatgcctc cactagcaat aaataaaaat aaagatgatt gatctgttaa
     1861 acgctcacct agaaaaccaa ataagcttgt gaaagttaac caagggataa taagcatcgg
     1921 atcaagcggg ccatcaaaat cgaatacatc tccaaaaata atcaatagga ctgccaatcc
     1981 tgcacaaatc agcaatgtat accaataaat cgtctcaagt gcgattcccc ctatcatata
     2041 cacgcctcct tccatttcta cgtctatcat accaatgttt gaaaattctg tatagcttct
     2101 tttaccgact cagtggtgtt ttttatagga aggttgtgtt tatcgttgac aatttgttaa
     2161 atttttgttt tcatgttaaa attttcacaa taaattcttt cgaggtagcc tatgtttcct
     2221 ttttttaaga aaaaaccatt aaagctccag aacacgatca tccttgtcgt ttgtttctcc
     2281 acattcgttt ctttgattgt ttctgccata ttgatcagga attttattct ttcgatgaac
     2341 cgatctagtg tgtcagataa aatctctagc attgctaaaa tcgttgccaa tgataaactc
     2401 atccaaaaaa agctgcagac tggtgacccg acacatgcga ttcaaggaat aacaaatgaa
     2461 atcatgattg catcgaattt agattttatc gttgtcatgg accctgaagg cattcgatat
     2521 tctcacgcag acccaagtta catcggtctt cctttctcaa gtcatgaaga tgtgaaaaaa
     2581 gtgattggag gacaagaatt tttctctgaa aaagaaggaa tgttagggtt cggcttccgc
     2641 tatttcgtcc ctatttatta tgatcaagag attattggtg cagtttgtgt tggcttaaca
     2701 aacaacacga ttgacaatca agtcaaagat ggacaaaaaa tgattgtttt ttcattgatt
     2761 attggactta gcgtaggcat cataggcgcc gtttttcttg cccaaaaaat gaaaaaagta
     2821 ctgcttggac ttgaaccaca agaaatagca gaacagttaa gtgaaaaaac gattatcgaa
     2881 aatgaagtag atgaaggaat cttagccatc tcttctgata aaactatcct cttgatcaat
     2941 acacctgcaa aaaacatttt ctaccaaaca gagcagctat tggatttcga agtaggaaaa
     3001 aaatcagtga taatctatat gacgttttgt ttaaagagac gattgagcat caaacgaccg
     3061 ttcggactca atccatgtat ttaaatggga tagaaactat ttctagcgtt acacctcttt
     3121 ttagcaatga ccgtttttta ggagcagtcg caactttacg aaatcgatca gaagtaatga
     3181 aattaatgga ggagcttggg gggacgaaac aatatataaa ctccatgaga gctcaaaccc
     3241 atgaattcat gaataaactt catgtcattt ctggtttgat tgatttgaag aagtatgaag
     3301 aagtcaccca ttttatccag cagctaaatc aaaattataa aaaagagatt ggccaagtct
     3361 caagtctgat caaagtacct gcaattgccg gttttttatt aggcaaaata aacgaagcca
     3421 aagaacaatc tgttgaaatg ctgttagaaa gcgattcttt tatccctaaa atggagatgg
     3481 atgactccat ccattcactt ttgcaagtgt tagggaactt acttgataat gcaaaagaag
     3541 ccgtgctaca aaaacatgaa agaaacggac aggtccggat tcatttaaac tatgacacag
     3601 aagctgaagt attcatcgtg accgtctgcg atgacggtgt cgggatatca gaagaaatct
     3661 cagaaaaaat atttacgcta ggtttttcga cgaagggaat caatcgtggg tttggattga
     3721 atctcatcca ttcaatcgtc aaacgccacg gtggattttt agaattttcc aaacaaacaa
     3781 atggcggtac aatcgtctat ctagaattac caaaaaagga agatcaaaat gatgacaaat
     3841 gtcctgataa tcgaagatga tccaatggtt gcagaattga atcgtacttt tgtggaaagc
     3901 atccctaata tgaacgtaat cgaaagtttc agaaatgtag aagaaggaaa aaaatatttc
     3961 agacaaaaaa acgcagatct gctgctgctt gatgtataca tgcctggcga aagtggtatc
     4021 cattttttat cttggcttag acagcaagga aatgatatcc cagtgatttt gatcactgct
     4081 gcagatgata tcgaaaccat aaagaatgtg atccgctacg gtgccttaga ttatttgatc
     4141 aaaccgttca ctttgcaacg ttttcagctg gcgatcgaaa agtatcttgc tttctctcag
     4201 acaatccaaa cgactgaaaa agccgatcag aaattgatcg atcaagtatt caaccacacc
     4261 actcccattc acgaaatacc atcattcatc gatttgccta aaggtctgtc aaaactgact
     4321 ttaaaaaaaa taagtgcatt tattctaaaa gaagatacta ttttttcaac aaaagaactg
     4381 gctcaaaaaa gctcactgtc acgtatatcc gtcaagaaat atttgaattt tttagtagaa
     4441 caagatatat tgaacgaaag aatcaaatat ttggaaatcg gccggccgct aacctgttat
     4501 gaattgacaa agaatgggcg gaaaaagctc acagattact gttgagtcat cgaattatcc
     4561 catacaaaat agtccaagta tacagaagta acaactgttt acttggacta tttttaaaca
     4621 ttttattgac gaatatccat gttttcttat cttaattgac gggctttagg aagagatacc
     4681 tcatttttcg tttttccagt ttctattatt tcacgaaagt tctcgaaact tgtcgaaatc
     4741 atatttgata atgcctcatc tgtatttgaa cctacatgcg gggttacaag aactctaggg
     4801 tacatttcta ctaattgctg gatagcaggg tctggaatcg tctcccatgg cttgaaggat
     4861 cggaaaaaaa cctctgtttc atttgcaaaa acatcggttg caaaaccagc taaatgattg
     4921 cttttcaaag ctcttaaaat agcttcatta tcttgaagct ctccacgggc tgtattaatc
     4981 aagatgctac cttttttcat ttgagctaaa aatccttcat tgatcatttg atcgttttca
     5041 cctgggatat gaggtacatg aatactgaca atatcgcttt tggctaataa ctcttcaagc
     5101 gagtggaacg tcaatacttt tttagcagct tctgtctgat gaatatcgta gccgatcact
     5161 ttggctccta gtcccttaaa caaaacagct tccgtcaacc ctatcctgcc tgttcctatg
     5221 atccctactg tgcaatttct gatttcacga ctaaacattt gctcatccac tcgaaaatct
     5281 ttgaaagatg ttcggatcgt cgtatacgca gtatttctca aaagcatcat ggcaaaagtc
     5341 aaagataact ctgctatcgc atttggcgaa tacgatggta cacgggctac tgtcatacca
     5401 aaatcagctg ccgcttgtag atcgatatga ttgactccca cagtccgtgt aaaaacataa
     5461 cgaatcccat attcctgcat ttttgcaaga ttggtctcat ctgctataca atttcccctc
     5521 aacaatacag catcatggtc ctttgctgac acgatattgt catgtgtcaa cagttcttca
     5581 taaagactga gatcataatg gtaaatattc aatttttcaa aaaaatctat ctcgttcgga
     5641 cgaacaccgt aacatgcgat tctcatactt tcttcctcca tttcttatga aaataaacct
     5701 atgccatgaa tcatttgtaa tacaacgatc catacaggtg tcagtaccac agcaaaaata
     5761 gttgacagta aggatgcatt ggaggctaaa agtgcctccc tatcaaaact gatagcatag
     5821 gctgctgcca ctgttgcagc tggtgtagcc atcatgataa cgattgttgc cagtgcttca
     5881 aaattgactg gtagaatatt cgttgccgtc aaaactgcta gaatcacgat attcaatgca
     5941 ggtacaaata ccactttgac tagactgtaa taccatgaag ttttttcaga tgcagctttt
     6001 ttgaagctta cttcacctaa tgttgcgccg attgctagcc acgccaatgg tgaacttaaa
     6061 ccagctaaat aatccatcgg ctgaaatagc cacggtgcag tctggtcaat tcgcaaaaac
     6121 gctacttggc ttatctctcc ttctgcacct gtaactgaca cttgtggcag atacccttgg
     6181 aacagccaaa tgaaaaggcc ggcaaaagta gcgataatga ttggattgac gaacatcgct
     6241 ttgatatttt tagcttccat cttcaagcca ctcattttta tgtatccata agaatacaaa
     6301 aagattcggt agccaatatt gaaaattgag gaatacatca cgccgacagg accataaatc
     6361 gcactgacga taggcgttcc gaaaaaggtc gtagaaccaa agatcgtcag gacacgcaac
     6421 gttgtttgct tatccccttt atagcttaag aaaaaaggtt ttgaaactag aataagaata
     6481 atataaataa ctaatcccca aatcaagaca ttgattcctt gcatcagtaa ttctgtattg
     6541 atatcttgca tgaaagctcg aaatgccagt gctggcaaag ccactgttaa gacgactttc
     6601 gatagaattt ttccaacaga ttcagaaaaa atcccctttt ttctacaaac aaaccctaat
     6661 aatataataa aaactgttga agtgattgca ctgacaatcg acatatctgt caaagtcgat
     6721 cgaaaaatgt caaataagtt catctttctc ctcctaatta tgttcattta ttcacaataa
     6781 aaaaagcttt tattttgcta atacgtatta acatttttaa ttttatcttt acattgttac
     6841 ttattgaaca ataggaaatt aaagaaactt ttgaaactta tgtaaagtga tttttactag
     6901 aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt aacttaaatt
     6961 atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg tcatatcagg
     7021 gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg tatcatcgat
     7081 tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg gtgttgctaa
     7141 tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc cagctaagtt
     7201 gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc ctaaattgta
     7261 atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga catgatcatg
     7321 tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc catcacaaat
     7381 caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg gttcgcgatg
     7441 attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg cttcaacagc
     7501 tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta ctccttcttc
     7561 agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag caattttttt
     7621 gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag gatccccgaa
     7681 ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt caaaatagat
     7741 tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct cagctacatc
     7801 ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca ctccacaaga
     7861 taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta catcatgatt
     7921 catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc cgctttggtc
     7981 aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa gtccatcggt
     8041 aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt cagcatagat
     8101 aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact cttatttaga
     8161 gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt tcacagcttg
     8221 tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt atattaagct
     8281 tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag cccaatagat
     8341 tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata agaaatttcc
     8401 ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg attgatgcag
     8461 cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg ccacaccata
     8521 tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga ttctttcttt
     8581 ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga tattttgacg
     8641 caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag ttgcttgata
     8701 cagattttca gaaatatcag aagattttag tacttgttct aacttccgat aatcacgttt
     8761 ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag tttttcgaga
     8821 gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca tcatcaccaa
     8881 tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg aacaaatatc
     8941 atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa tgatcgttcc
     9001 aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat tcgcattcta
     9061 ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact aaataatata
     9121 ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg tctgtggccc
     9181 tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta ggaattctct
     9241 cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga ttcgaaaatg
     9301 atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat gttcatgtaa
     9361 aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat caatttttgg
     9421 gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat cattgattgc
     9481 attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca gctcatcgac
     9541 actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat tggaacacaa
     9601 acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg acttatctcc
     9661 acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg atagaccgtt
     9721 cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt ctagaccatc
     9781 catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt gtcgagctac
     9841 ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat tattaggatt
     9901 aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat caatatcagg
     9961 ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag ctaatgcaat
    10021 agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc cattgtccag
    10081 caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt agatatcatt
    10141 gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt ttctagcaga
    10201 aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga tcaaatcacg
    10261 aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt tcaattttaa
    10321 aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac cgcgaacatc
    10381 gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt agccaatccc
    10441 tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca atatcatact
    10501 ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa aaacacaaac
    10561 aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc caatccattg
    10621 caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat gcaagtccaa
    10681 tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc tccatcttaa
    10741 taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat tagtgtgtta
    10801 ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat gtgagcgtta
    10861 gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa ttttgcttca
    10921 cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg tatcctttac
    10981 aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc ggaaacaagc
    11041 tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa aatccctcac
    11101 ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta ttataaaggt
    11161 acaaatacca agcacggtta atccaaattg cataaattca tttttttgta cactcatgct
    11221 tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat aaagtatata
    11281 gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta accttgaaca
    11341 aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt caaaacttaa
    11401 ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag ctatccaagt
    11461 acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat tcaggttact
    11521 actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt gacccattgt
    11581 attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata tacgcacata
    11641 atagttttga catttcctat atgttagaag gaaaattaag aaaaa
//
LOCUS       DAJSVF010000078        11635 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSVF010000078 DAJSVF010000000
VERSION     DAJSVF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.081"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            107..310
                     /locus_tag="ON550_002152"
     CDS             107..310
                     /locus_tag="ON550_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3955223.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(411..1073)
                     /locus_tag="ON550_002153"
     CDS             complement(411..1073)
                     /locus_tag="ON550_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955224.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(1086..2588)
                     /locus_tag="ON550_002154"
     CDS             complement(1086..2588)
                     /locus_tag="ON550_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3955225.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3136..4815
                     /locus_tag="ON550_002155"
     CDS             3136..4815
                     /locus_tag="ON550_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3955226.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4942..5421
                     /locus_tag="ON550_002156"
     CDS             4942..5421
                     /locus_tag="ON550_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR3955227.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5640..6320
                     /locus_tag="ON550_002157"
     CDS             5640..6320
                     /locus_tag="ON550_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3955228.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            6328..7659
                     /locus_tag="ON550_002158"
     CDS             6328..7659
                     /locus_tag="ON550_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3955229.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7674..8636
                     /locus_tag="ON550_002159"
     CDS             7674..8636
                     /locus_tag="ON550_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR3955230.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8747..9085
                     /locus_tag="ON550_002160"
     CDS             8747..9085
                     /locus_tag="ON550_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR3955231.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            9164..9871
                     /locus_tag="ON550_002161"
     CDS             9164..9871
                     /locus_tag="ON550_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR3955232.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9974..11455)
                     /locus_tag="ON550_002162"
     CDS             complement(9974..11455)
                     /locus_tag="ON550_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955233.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
BASE COUNT         3717 a         2013 c         2426 g         3479 t
ORIGIN      
        1 cagctctaac tatctttttt agaaaacgag cgttctgttt gtctgtatct gttgttgatg
       61 ttaccctgaa ggtagatcaa taaacaaact aagaggagga agcacaatgg cagatttaaa
      121 aggacgttct aaagatatga aagataaagt agtcggtgaa ggaaaagaag catacggcaa
      181 agcaacagac gacaagagca aagaaacaga agggaaagca caatcactag gtgctgacat
      241 caaagaaaaa gctcgaaatt taggcgacga agtcaaagac aagttttctc gaaaagacga
      301 aaaaaaataa aaaccactta ttactaattg aatcaagcaa ataaaaaatc cgaaccatat
      361 ctgcgagaca gatatggttc ggatttttta tttgatcatt atcctgtttt ttaaacttcg
      421 attttttgaa attttttatt ttttagttca aagacttcat cacagctttt tgctacttca
      481 atctcatgag tcacgatgat aatacagcga ttttgttcat gggcgatttc ttggaaaagt
      541 ttgacgatat cttgtgaagt tgtttcatct agatttccag taggctcatc cgctacgatt
      601 agttgatgtt cacagcagat agcacgaacg atagcgactc gctgctgctg acctcccgaa
      661 agtttggtca cctgtttttt tgctaatatt tcatcaattc ctacacgagc cagtcgatct
      721 aaagcaactt gtcgataatt ctcttgcttg gtcttagtga tttccattgc tgctatgacg
      781 ttgtctagag ccgataaata aggaagcaag ttgtaagctt ggaaaataat cgagacatca
      841 tttttacgat aacttcttaa cccgattttg ttcagtgaac gatcttgata gtataccttt
      901 ccttcttttg gtgcatctag tccagaaata agcgaaagaa aagtcgtttt tccagaacca
      961 cttgctccta aaatagcgta catttttcca gcttcgaaat tcagttctac atctttataa
     1021 agcgcatctt gcggatcttg ataccaaaat cccaattgtt ctgtccgtaa catcaaattc
     1081 ctcctttagt ttaataggat ttttcttggg tttaatctta atatccctat agaagctaaa
     1141 aagacagaaa acagactgat tccaaatgct actccggcta atagtcccaa ttgtgcaggt
     1201 ttgacattga tttccaattc gctgacttga tcagaaacag caaatggatt attcgtggtt
     1261 ccttgttgac catcgcgttg accgccttgt tcttgtctgt tacctggcat ttgtccaccg
     1321 ccaggagctt gctggttttg tgtcgtggtt tctgtctgtt ggtcaagtag ctgctgtccc
     1381 acggcattcg cgacgacgtt tccgctaagc gaagcaactc ctaatgcaag aatcatacaa
     1441 atagccactt cggtaaacaa ttggagaatg attttacttc tggattctcc taaagatagc
     1501 aagacgccta actcatgccg acgctcccga atggtaatca taatgatcag agtaagtatg
     1561 ataataccgg ctacagccac tagtaaaaca acatttttag aaaaacttgc cacgttgttt
     1621 aaaggttcca acattgactg atacatggaa tcatttgatt gtaagctaaa tgtatctgtg
     1681 tcgatcagct tctcagcttc tttgagaaac tcatccattt tatctggatc agatagagtg
     1741 taaatagcag aatctatcgt gttatcgtca gatgtcccat ttagttcatt tgcaaaagtg
     1801 taagaagtgt aaagtgtatt cgacggattc atgaagttga atttcatacc catactgccg
     1861 cttgtttcgc tggattcata aattcctttg atcgtcattt cataagtggt atcttcatct
     1921 tcagtactcg tgattgtgaa agtatctccg actgccaggt catttgtttc tgctagagta
     1981 gaatcaatga ctacattgtt tgtgcctttg tcgctgtcat ctattccttc accatccgtg
     2041 atttttgctg ttccatcaga aaaattacta ttttgtgcag ttgctgacac accactgatt
     2101 tgaaagtctg cttgagtcat ttgcggattc ccgcccattc ctccagcagg ttcaccgcct
     2161 gcatcagtag tttctgaact gtcttcactt gaatcagaac tagtgatggc agttattcca
     2221 tcttttgcca tagctgaggc ggaactttca aaggaataac tctttacatt atctaattca
     2281 gctatttttt ccgcgtccgt taaggaaact ggtgtaagag aaaaactgcc tggatctgga
     2341 cgttcttcag tggtagaacc gcttgaagaa gtttgttcct gttttttaaa tgaagcttct
     2401 cgattggcac ttaaagtcac tgttgctcca acttcttttt gtgcttgatt cgctgcttct
     2461 tctgcagcgc ttcgaatcgt gaggccagct agcacaaaaa taagaatggc tgtgaacacg
     2521 gcaaacaata acaatgaacg tccccatttt acttttgtac ttttccatgc acgtttcata
     2581 aaattcatat tttgcatccc tcctagtaga tgcatttgat aatagacaac ctatcttaaa
     2641 gctagcttaa atcttatgct gtttgcacac catcaacatg aggttctgta tatataaaaa
     2701 gtcactatag ttattgcaaa aaatgtggta aaatgaaaaa agtatgtttc aaaccgacga
     2761 agaagaaatg aaagaggaat agcaaaagaa atcctagatg ctacagaggg atttttacgt
     2821 gcatagttgt tctaacaatt gctgatacta gcgatttagt ttatttgttg tataataatg
     2881 gggaacagct gaatacttat ataagtattc tggctatagc gtttcgcaaa agatccattt
     2941 tgttaatgaa gtatatccaa aataaaatgc ccatccaact gtcagaacgc ggatgtgctg
     3001 gtaattttcc tgctgattct ccataagttg agaaatagtg attttgcata tgcttcattg
     3061 gaatggctca tatatttaga taaagaagaa aatcgacatc tttaaacttg gatgtcataa
     3121 gaagaggtga agtttgtgag taccattaaa attatcccat taggcggcgt tcgtgaaaat
     3181 ggaaaaaaca tgtatgtcgc agaagtgggc gaggatattt ttattttaga ttgtgggcta
     3241 aaatatcctg aaaatgagtt gttggggatc gacgtcgtaa tccctgattt tacgtattta
     3301 gaagaaaaca ttgatcgtgt agctggtgtc tttttaacac atggacatgc agatgcgatc
     3361 ggggcacttc cttacttgtt gtcaaaaatt tctgtaccag tattcgggac agagttgacg
     3421 atcgagttag caaaattgaa tgtcagtcgt aatgaagcag ccaaaggatt taaggatttc
     3481 catgtggtag atgagcatac agaaatagac tttggtgaaa cagtagtgag ctttttccgt
     3541 accactcata cgataccaga ttcagtgggg atcaatctga aaacaaaaga aggtagtatc
     3601 gtttatacgg gagattttaa atttgaccaa acggctatcc caatgtatca aacagattat
     3661 gcacgattgg cggaaatcgg caaagaagga gtcttagcct tactaagtga atcggcaaat
     3721 gcagaaaatc ctcagcctgt agcttctgaa gcccaaatag cagatgaagt atttgatacg
     3781 atcaaatatt gggaagggcg tatcatcgtc gcttgtgtag caagtaattt gcaacgagtg
     3841 caacaagtat tggatgcagc agaaaaagca caacgaaaag ttgtcttgac tggtcaagat
     3901 ttcgaacgaa ttattcgcac agcgatgcgt ttagaaaaac ttcaattacc aagtgaagat
     3961 ctgcttgtta cgttgaaaga catgaaaaac tatgatccag aagaactgat cattttagaa
     4021 actggccgaa tgggtgaacc catcaagtct ctgcaaaaaa tggcaaacgg tacacaccgt
     4081 aatgtcaaga ttgaagaagg agatttggtt tatatcacta ctacgccaag catcgcgatg
     4141 gaaaccatcg tggcgaaaac ggaagatatc atttatcgag ctggtggaac agtgaagttg
     4201 atctctgaga atatgcgcgt ttctggccat gccaatccta atgacttgca attaatgatc
     4261 aatttgatga aaccaacata ttttgtgcca gttcaaggag agtatcgtga attagctgct
     4321 catgcagatt tggctcatgc agtagggatg ccatacaaaa atatctatat tactggacgc
     4381 ggagacgtgc tggaatataa gaacaagcgt atgagtgttg caggaactac aagtgcagaa
     4441 aatgtaatga ttgatggatt aggcataggt gatatcggaa acattgtttt acgtgatcga
     4501 aaaatcttgt cagaagacgg gattttcgta gcggttgtca ctatcagtcg gcgagaaaag
     4561 aaaatcattt caaaacctca aattacttca cggggattcg tttacgtaaa agctagccgt
     4621 gatctgatga aagaaagcag caatatgatt gaaacaatcg ttgagaaaca tttagaaagc
     4681 aatgattttg agtggagcaa attaaagcaa gacatccgag atcaattagg ccgttactta
     4741 tttgaacaaa caaaacgccg cccagtgatc ttgccagtca ttatggaagc tactcaacgt
     4801 cgaaataaaa aataaacaaa aagagtaaga tgaacctctc tatagagtgc tcgtcttgct
     4861 ctttttttgc gaaaaacaaa taatttttga atacagcatc cctcgcttta taataaacga
     4921 tagaataaag ggagtgaatc aatgatgagt caaacaaaag tcgaacgtta tttagaagaa
     4981 caaaagatca gctatcaacc acttgatttt agtcatgtgg tagaagaatc tttcacagaa
     5041 gaactacaaa aaagagggat tgatccagct ttgatatgta aaactctcgt agtgaaagga
     5101 aacaaaactg gggtgatcat cgcattgatt cctttgaacg atcacttaga ttacaaaaaa
     5161 acgagacaag tgacgaaaga tcgtaaagtt ggttttcctg gaatggattt tgtattaatg
     5221 catacaggat atccccacgg agcaaacacc cctattggaa taaaactcgc acatcctgat
     5281 tattgttttt tagcagacag ttctttaaaa gaaaaagaag aagtaattgt gtcaagcggt
     5341 gaaatcggga gaagccttcg gataaaagta gccgatttag aaaagatcgt ccatcctatt
     5401 tacgctgatt taattagtta agggacgaga agagaagcta agcagagcag agacctcaaa
     5461 cttgagatta tatctgcttg gtacttggtg catcctgtat tatctgttac aattgctttg
     5521 tacatactgt actatcaatc caatgaaaat atatttagca gctatatttt ttcattgatg
     5581 tgcaagtgat gaaatcgttt ttaataaaaa agatacagag aaaacgcgag gtgtaaaata
     5641 tgaaaattac gtatcatgga cattcctgtt taacaattga aatggaaaat ggccaacgcg
     5701 tgatgattga cccgtttatt acaggaaatc cattgacaga cttaactgca gaagaagtaa
     5761 agacagattg gttgctgatt actcatggtc atagtgacca cataggagat atgattccta
     5821 ttgcaaaaga aaatcaagcc acaattatca gtattgttga gattgctgat tatgcgaaca
     5881 gcagaggagt agattcattc ggtatgaata tcggtggaaa acatgcgttt ccattcggcg
     5941 tggtaaaatt cgtacatgca cagcatagtt caagctatga agtagacggt atcgttcagt
     6001 atatgggcga gcctagcgga attattattc aagcagaagg aaaaacgatt tatcatgcag
     6061 gggatactgc ttatttcagt gatctaggat tgcttgcaga agaattcgat attgatgtcg
     6121 ctttcttgcc gattggcgat aactatacga tggggccaga agatgcagca aaggctgcgc
     6181 aaacaattcg tgccaagaaa gtagttccaa tccattacaa tactttccct gtcattgaac
     6241 aagatcccga atcttttgta gagttactgg aagatagtga aggaaaaata atgagcgtct
     6301 cggaaacgct ggaagtgtag gcagaaaatg gcgacaaaac atgatttgat tttagaatat
     6361 attgaaagtt tacctgttgg caatcgaatt tctgtaagaa gcatcgcaaa agaattgaat
     6421 gtcagtgaag gaacagcgta ccgtgcaatc aaagatgcag aaaatgtcgg actagtttca
     6481 acaattcaac gagtagggac catccgtatc gaacgcaaat taaaaaaaca tattgaacgt
     6541 ttgacatttg gcgaggtcgt caggatcatt gaaggggacg ttttaggcgg ggcggcagga
     6601 ttagacaagg tcttgaataa attcgtcatc ggcgcgatga ctaaacatgc catggaacgc
     6661 tatattacgc caggatcact aatgatcgtc ggcaaccggg cggaagtcca aaaattagca
     6721 ttggaagatg gcgccgcagt attgatcaca ggaggattca atacgactgc tgagattgca
     6781 aagctagctg atgatcttga aatgccgatc ttacgcacct cttatgatac atttacagtc
     6841 gcaaccatga tcaaccgagc gttaagcgac caattgatca aaaaagatat catgcttgtc
     6901 agtgatatct atatgccagc tgaaaagact cattatttgc agcagacaga tacgatttct
     6961 gattatcagc gtttgtcaga agaaacgcag cattcgcgtt ttccagtcgt caaccgccat
     7021 catcgtttga tggggattgt tacggcaaaa gatgttttag ggaaatcgcc aaaccagatc
     7081 atcgatcggg tgatgacgaa agaaccaatc agtgtaaaga aaacaatgag tattgcctct
     7141 gttagccatc aaatgatctg ggatgggcta gaagtcatgc cggtcgtttc cgatgacttg
     7201 acattggaag gcttgattac ccggcaagat gtaatgaaag cgatgcagct tgtccagcga
     7261 caaccgcaga ttgcagatac gatttcggat cagatatctg gaaatatcca tacgattgat
     7321 actgaccgtg aaggaaatcg tttggaaaac cctaagttca aattcgtagt agcaccacaa
     7381 atggtcaatg gcgttggaac catttctttt ggcgtactta gcgaaatcat ttctgatgtc
     7441 gcacaaaaaa caatggtgat gaaccaaaaa cgaaatatct tgattgaaca agtgaaccta
     7501 cattatttgc ggctgatcca attggaaagc gaattagaca ttcgtccacg tgtattggaa
     7561 atcggcagac gttccgctaa actggatatc gaagtgttta ttgaaaacac catcgtttcc
     7621 aaagcaatcg ttgtttgtca agtaatggaa cgttcttaga aagcaggagt gaaatgacag
     7681 tacagcaaga aatagtagat acaataaaaa aatacgataa aatcattatc cacaggcatt
     7741 tgcgccctga tcctgatgca ttaggatcac aagtaggatt agccgaacta ttacgggaaa
     7801 gtttcccaga aaaagaaata cgtcaagtag gtgaaacgat tgaagggctt cgctttttag
     7861 cagaaatgca agaagttgaa gatgtcttct accaaggagc gctagtcatc gtgactgata
     7921 cagctaattc acccagaatc agtgatcaaa gatatcagtt ggcagaaaaa ctgatcaaaa
     7981 tcgaccatca tcccaatgat gacccttatg gagatcttgt gtgggtaaat acaaaagcaa
     8041 gtagctgtag tgagatgatt gcagaatttg ctttgatgtt tcctgaatct ttaaagatga
     8101 acgttaaggc tgcacggtta ctttatgcag gaatcgtcgg agatacaggg ctctttttgt
     8161 atccctccac gacgtcccgt acattagaga ttgcttcgat attgagaaat tatccatttg
     8221 atgcaagtgc tttgaacaga gagattgaac aaatgccgat gaaagtcgct aaactttccg
     8281 gttatcttta tcaaaatatc caggtggatg aaaatggtgc aggaagaata atccttccgc
     8341 aagaaattct tgataaatat ggaatcgatg attcagagac agcttcgatc gtttcacttc
     8401 ctggagtgat tgacgaagtg ctggcttggg ggatttttgt tcagcagcct tcagggtatt
     8461 accgcgtacg tttacgttct aaggggccga tcatcaatga attggctaaa cgccatcatg
     8521 gaggaggcca tccattagca agtggtgcga atgcaaaaga tttagcagaa gtagaggaaa
     8581 tctataaaga gatccaagat atatgccaat cgtataagag cagaaaaaca gaatgatact
     8641 tcgttcgaaa acgtgccttt tgtagtaaaa atcacaaatg tatcttttca agatcttctt
     8701 tttccgttac aatagtgctc actaaagatt ttaaaggaga aagaagatga cattaccaaa
     8761 ttgcccagaa tgtggctcag agtacacata tgaagatcga ggcttgttga tttgcccaga
     8821 atgcgggaac gaatggaaca aagaagaaca agaaactgtc atagaagacg gattagtggt
     8881 attagatgca aatggaaacc gtctgttaga gggcgatagc gtgacagtga ttaaagactt
     8941 gaaagtaaaa ggtgcgtcag gtgccattaa acaaggaaca aaagtcaaga acatccgttt
     9001 agtagaaggc gatcataata tcgattgcaa ggtagaagga tttggaccga tgaaactgaa
     9061 atcggaattt gtgaaaaaga attaaagaat attcaaggtc ttttcacatt cttttcctac
     9121 tactaatgat aaaataaagt aaacgaaaag gagtgaaacg aaaatgtatg gttatggaat
     9181 gtatggtttt ttagatccga catttctttt ggtcattctt ggattagtta tttccggaat
     9241 tgcgtcagcg tacgtcaata gtactttccg aaaatacgat caagtgagaa gcaaaaacaa
     9301 tgtgactggt acacaagcag cacaatatat attacaaagt caacagataa ataatgtcgg
     9361 tgttcaacaa attgccggag atctcacaga taactacaat tcaggtaata agatgttgag
     9421 tttgtctcaa gcaactgcac agtctacttc agttgctgcg ataggtgtag cagctcatga
     9481 atgcggacat gcggtacaag atgcttcggg ttatgtccca ttgaagattc gtgcagcact
     9541 tgttccagca gctaactttg gttcgatgat ttcgttcccc ttgattatgg taggtgtatt
     9601 gcttagctgg aactctacgt taatcaatat cgggattttc gctttttctt tagcattact
     9661 tttccaattg gtgacattac cagtcgaatt taacgcgtct cggcgagcaa tccaaatttt
     9721 gagtgaaggc ggactactga cggaagaaga agtaccgatg gcaagacacg tattatttgc
     9781 agcagcactg acttatgttg cagcagcgct ttcgacattc ctgcagctgt tgcgcttgat
     9841 cttattgttc ggcggcaacc gaagagatta aaataaagaa gttgtgaaat cagcgctttg
     9901 atctgagcta ataccgagag ttagctgatt gaataccatt tatttggaat aagaggatgt
     9961 gacaagaatt ttgtcacatc ctctttgttt tgttctcata accttttttc atatacagtt
    10021 tatgaaccgc ttttattaac ttgccaaata acactgtatt gtgcgttgta tccagataga
    10081 aagtctccgt ttcatctgaa attttctcat gctgatctgt aatgatcaaa tcagcatgtt
    10141 ctgaatatgc ggtaatttta acagcatctt tgcgaaataa ctgctgcaaa tggctttgaa
    10201 gatgataatt tcctgtaaac tgtttggtta tctgcaaatg gatgagtaac tgctctttcg
    10261 tttctgtttg tagtaagtgt tggatcaatt gactaaggta ttgactgatt tggtttcttt
    10321 ccccattttt tgaaaaaatt gtatcgattt tttctcttaa tttcttttcc tgtataaaag
    10381 aactagaatg attggacgtg gcttctggaa agcaaagtgt agaaaaacta ctgctggatt
    10441 cacccaacag ttcgaaaaaa acaactgcca acacaagaaa ataaaaataa attggtattt
    10501 tattttctaa atggttgaga ggaaactgtt gagttaagta agtcatcaat tgaccgactt
    10561 gtcgacaaat aaggtttttg cttgtggaaa ataaaaaacc aatctcctgt ttttgtcgaa
    10621 aagagatttg atttggaaaa aaacaataga aaaaataatt aaagtaggct ctctctgttt
    10681 ctgtctcttc acaagggcga ctcactgaaa aagaagtaaa gtattctgaa taatcatgtg
    10741 tatgctgcaa taagtccatc aattcagtga tttcagaaga agtttttgga aaatatgcct
    10801 gttgtttgtt tcgtaatatg aataaagtta acagcaagta gatatttttt tcttgttttg
    10861 aaattttttc tatttgctca tctgtactgt tcatttttag tgcatttaat tcaagtgagg
    10921 aaaatggcca atcgttacct ccaaatgtat ctgaaagaaa aaagtaaaga tagagacgga
    10981 tagataactc atttccttca agagtacagc aattttttac agataggatc cgtaaatgga
    11041 atttttttaa ggaatgattg agctgttttg tcagtttttc gagataggag ggcgatatat
    11101 aaagttgttc aagtaaatat ttttttggta cagcagtttg tggataattg attagtaaca
    11161 tcaaatagtt aaaacgattg gattctttta ggtagcataa ttttaactta gaaaaaacag
    11221 ctaacttttt agtttgatcg gtttctttgg tttggatctc tcctttatga ttagaataaa
    11281 taaattcatc aatcaataaa aattgcttga gtgatacatt tatttttcgg atcagttctt
    11341 tggttgtccg ttttggaata tgtaagtcat tggataattc ttgtaaagaa caattatttt
    11401 tttgaatgac catttgtaga acaagtaact ctttttttct tgcttcaatt agcataaata
    11461 cctccttttt attctgtttt tgtatatagt tagacaaaaa ctgtgagtgg aaagtcattt
    11521 cagtctgtgt tattctcaca gagtgtcgcc ttctttttta tataacgtta tttaattttt
    11581 ttgttacctt gtttattcta ttgtttattt cctagggtat tttgtcaaga tattc
//
LOCUS       DAJSVF010000079        11615 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSVF010000079 DAJSVF010000000
VERSION     DAJSVF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.082"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(311..796)
                     /locus_tag="ON550_002163"
     CDS             complement(311..796)
                     /locus_tag="ON550_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR3955234.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    886..1231
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1276..1917
                     /locus_tag="ON550_002164"
     CDS             1276..1917
                     /locus_tag="ON550_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3955235.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1936..2571
                     /locus_tag="ON550_002165"
     CDS             1936..2571
                     /locus_tag="ON550_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3955236.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2589..3404
                     /locus_tag="ON550_002166"
     CDS             2589..3404
                     /locus_tag="ON550_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3955237.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3723..5159
                     /locus_tag="ON550_002167"
     CDS             3723..5159
                     /locus_tag="ON550_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3955238.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5348..7795
                     /locus_tag="ON550_002168"
     CDS             5348..7795
                     /locus_tag="ON550_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3955239.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            8025..9092
                     /gene="rlmN"
                     /locus_tag="ON550_002169"
     CDS             8025..9092
                     /gene="rlmN"
                     /locus_tag="ON550_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3955240.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9285..10388)
                     /locus_tag="ON550_002170"
     CDS             complement(9285..10388)
                     /locus_tag="ON550_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3955241.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10941..11489
                     /gene="aac(6')"
                     /locus_tag="ON550_002171"
     CDS             10941..11489
                     /gene="aac(6')"
                     /locus_tag="ON550_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3955242.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3866 a         1953 c         2497 g         3299 t
ORIGIN      
        1 tgtgctggga tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt
       61 taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt
      121 ttatatatgc ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa
      181 tactcgtatt agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa
      241 tttctgatat attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac
      301 tactctatca ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt
      361 caaagtcgga aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg
      421 tatcttgacg atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca
      481 tcaatgctaa tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt
      541 cagaaacaaa agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt
      601 gcttttcatc aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata
      661 gattttttac ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag
      721 gatgatcgac tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt
      781 cggttgcacg actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag
      841 ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa
      901 agaagagtag ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt
      961 aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag
     1021 cccgttacag ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt
     1081 cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt
     1141 ttgttttcat ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca
     1201 gaggtggaac cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta
     1261 aaggaggaaa agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca
     1321 ctcacaacat tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt
     1381 ttagcattag gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta
     1441 tgggttatgc ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg
     1501 acgattggga tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat
     1561 gcagcttatt ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat
     1621 gaagcagcgc aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca
     1681 caagtgacaa agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat
     1741 tcttccttga tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg
     1801 gaacgcgacg cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact
     1861 ggtattctaa caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg
     1921 ataggaggaa gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa
     1981 ataatcaaag atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct
     2041 tccggtggcg gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc
     2101 ggagaatttt atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa
     2161 gttgtaggag tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac
     2221 attaccttag caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca
     2281 gaagaattag cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt
     2341 tctggcggac aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta
     2401 ttggcttatg atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt
     2461 ttgatcctat cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta
     2521 tttgcagaaa atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga
     2581 agatagagat gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg
     2641 caggctgttc gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa
     2701 aaatcgttat aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga
     2761 agctcacagg atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg
     2821 tagactttca gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg
     2881 atttgatatg gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa
     2941 aaccttatat ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact
     3001 ttggacaaat gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat
     3061 ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg
     3121 atagttttaa tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg
     3181 ataaagtata cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa
     3241 caaaaggttc ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat
     3301 tagtgaaaaa aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa
     3361 tatccgataa atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg
     3421 acaaaagacg tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg
     3481 tgactcggag ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt
     3541 tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
     3601 tactactcga aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg
     3661 gaaggggttt cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat
     3721 gaatggaaca caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg
     3781 cagcgtatca agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc
     3841 gatttgtacg gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc
     3901 actatcatcg ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc
     3961 gcttctctgt agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg
     4021 ggctcgcttt ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt
     4081 tgactttata tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat
     4141 cacgtcagat cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg
     4201 ggaaagtcca ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt
     4261 tgttagcagc acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata
     4321 ttgctaattt agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa
     4381 aaatcggacc aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg
     4441 ttctggctgc tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt
     4501 ggggaaaata tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga
     4561 ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt
     4621 actatcaaac ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga
     4681 atacgacagg aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag
     4741 ttgagaatcc atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa
     4801 ggatcggact gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg
     4861 gcttaggaga gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca
     4921 attacttaca aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc
     4981 taggctattg cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc
     5041 gatatggatt tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata
     5101 agaaagacag ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt
     5161 tcacatactt aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc
     5221 atttagaaat taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt
     5281 tcaaacaaaa ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg
     5341 ataataaatg gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa
     5401 aaagcaaaaa cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt
     5461 atttgctgtc ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt
     5521 tcggatcatt gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt
     5581 ttggattgtc attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg
     5641 catactcttt tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca
     5701 tactggggaa ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca
     5761 aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac
     5821 ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg
     5881 agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg
     5941 agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc
     6001 aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa
     6061 agaagctgca gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag
     6121 ggaaaatact gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt
     6181 tgttccaatc gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc
     6241 agatacacca aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga
     6301 cggtacctca ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc
     6361 gacagttgat ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat
     6421 tgaaaaaaat atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt
     6481 cgtcaaagcc agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt
     6541 aaaagtaagt aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga
     6601 tgtccggatg gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg
     6661 aaagatcagt atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa
     6721 attactagaa gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc
     6781 gaagatgcct cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa
     6841 tgggattatc acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat
     6901 cgatccgaaa atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt
     6961 cgtgacgaac cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga
     7021 acgatatgaa aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt
     7081 tcaacagaaa aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat
     7141 cgtagatgaa ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat
     7201 tcgtttagcg caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc
     7261 aagtgtggat gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc
     7321 ggtatcaagc ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt
     7381 agggcgagga gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg
     7441 agcattcatt tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga
     7501 agctcattat gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga
     7561 acagccacaa gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc
     7621 aagtatttcc cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt
     7681 agatgaactg gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt
     7741 attcattgag caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt
     7801 gtgtgatgaa gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa
     7861 agaaaaaatg gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt
     7921 ttgtgagatt aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca
     7981 tgtatagtat gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca
     8041 tttatggctt gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat
     8101 tccgagctgc ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa
     8161 tgacgaatct atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc
     8221 taaaacagat ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc
     8281 ctgataaaaa catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg
     8341 tgacgacaca agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa
     8401 agaaccgtga tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt
     8461 ttgatgagcg caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc
     8521 catttgataa ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat
     8581 tggcaattgg tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg
     8641 aattcgctaa taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg
     8701 aggtgcgaac atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag
     8761 ctgtggatga gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga
     8821 atcaagtaaa tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga
     8881 aaaaattagc atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt
     8941 ctcgcagtcc aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa
     9001 actgtgtgat acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa
     9061 gcaagcaaat gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc
     9121 ataaaaggag cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct
     9181 atagcgaaag ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc
     9241 ctcaatttga tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta
     9301 tatccttgct ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta
     9361 aatttttctc gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta
     9421 tctaagattg cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga
     9481 agttggttct ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc
     9541 atcaataagg tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt
     9601 tctagaacag tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta
     9661 actaaaacag aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca
     9721 tatgagggac gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga
     9781 tcaatgcaca tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca
     9841 cgagcagaaa aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag
     9901 tatttatcag gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt
     9961 gaaggaaaga agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg
    10021 acgggcgaac agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca
    10081 ccaatgactt ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct
    10141 ttttggacac tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg
    10201 taccagagac tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct
    10261 gcctttgaaa gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg
    10321 attccatttg ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt
    10381 tgactcatat tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc
    10441 gatctgactg cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata
    10501 aggtggcatg aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa
    10561 cattgaaaac gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc
    10621 ttttttgtaa ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat
    10681 ttgtcaattc ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa
    10741 cagagttttt tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt
    10801 ctgtataatt tttttatttt ataagtaaga taaataatga aagaatagag atactgattg
    10861 gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc
    10921 aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga
    10981 aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg
    11041 cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag
    11101 atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc
    11161 atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt
    11221 acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt
    11281 tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg
    11341 cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa
    11401 tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa
    11461 cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct
    11521 tcaagaaata agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt
    11581 atttccgaag aagctacttt tggaacaccg tgtac
//
LOCUS       DAJSVF010000080        11480 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSVF010000080 DAJSVF010000000
VERSION     DAJSVF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.083"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(49..2124)
                     /locus_tag="ON550_002172"
     CDS             complement(49..2124)
                     /locus_tag="ON550_002172"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR3955243.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKITNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            complement(2126..3043)
                     /gene="glyQ"
                     /locus_tag="ON550_002173"
     CDS             complement(2126..3043)
                     /gene="glyQ"
                     /locus_tag="ON550_002173"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR3955244.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3427..4239)
                     /gene="recO"
                     /locus_tag="ON550_002174"
     CDS             complement(3427..4239)
                     /gene="recO"
                     /locus_tag="ON550_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR3955245.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4382..5281)
                     /gene="era"
                     /locus_tag="ON550_002175"
     CDS             complement(4382..5281)
                     /gene="era"
                     /locus_tag="ON550_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR3955246.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5296..5691)
                     /locus_tag="ON550_002176"
     CDS             complement(5296..5691)
                     /locus_tag="ON550_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR3955247.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5675..6151)
                     /gene="ybeY"
                     /locus_tag="ON550_002177"
     CDS             complement(5675..6151)
                     /gene="ybeY"
                     /locus_tag="ON550_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR3955248.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6168..8360)
                     /locus_tag="ON550_002178"
     CDS             complement(6168..8360)
                     /locus_tag="ON550_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR3955249.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8384..9355)
                     /locus_tag="ON550_002179"
     CDS             complement(8384..9355)
                     /locus_tag="ON550_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR3955250.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10061..11440
                     /gene="brnQ"
                     /locus_tag="ON550_002180"
     CDS             10061..11440
                     /gene="brnQ"
                     /locus_tag="ON550_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3955251.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3084 a         2421 c         2107 g         3868 t
ORIGIN      
        1 aaatagccta ttttgtgcct gttctctggt ttatttacat attagaaatt attttgtgat
       61 gatttgatct aaacttgcta gtgacaaagc catggcagca atttgattta attgcgttaa
      121 tcggttctta cgtactgctg gttcttctgc catgaccatc gtctgatcaa aataattttc
      181 gatcagtggg cgaaggctta caagctgatc ataattttcg ttgatcgttt ggtcagcaaa
      241 gcttgttttc attgcctcta ctgctttgtg cagttctttc tccgcttcgt tttcgaacag
      301 ttcagggtct acttctgatt gtttttcaag ctccgctttt ttcgctaagt tcacgacacg
      361 tgtcaaagct tccatcgatg gtttgaagtc ttcatctgca agatgctgct tgaacaattg
      421 tgctgctgca aatacttttg tcagatcttt ttgttctgcg ttcaaaacag cttcgatgac
      481 atcatgacga atattcttcg ttgtcaataa ttggcgcaga cggcctttga taaagtcgat
      541 cacctgttgt tgtcccgctg acaagccaat gccaaaacga tccgtatctt tactgataac
      601 ttcactaatc gtttcttgca gaacagacaa aggaaaggcc cattttttcg attcgatgat
      661 tcgaacaaca ccatatgctt gacggcgcaa agcatatgga tcgtttgagc cacttgggat
      721 catcccaact gcaaagaaag aaaagatact atctaattta tcagcaatcg ctaaaacaga
      781 acctactgta gtctcaggta gatctccttc actggatata ggcatgtaat gttcacggat
      841 cgctgttgct acggctggtt tctcgccttt aagcagagca tatttttctc ccatgatccc
      901 ttgtagttca gggaattctc ctaccatgtt ggttactaga tcaaatttat agatttcagc
      961 agcgcgttgc agatccatca attcttcctc agatagtcct acttgttcgc ctatgatgcg
     1021 agcgatgact tgtacccgtt gcattttctc gtagatgcta ccgatttttt catggaaagt
     1081 aacagttttt aatttttcaa cgtattggtc gattgaagct ttcttgtctt cattataaaa
     1141 gaattccgca tcttctaaac gagcagtcaa tactttttga ttgcctcgta ccacattttc
     1201 caattttacc cgatcgccat tacgagcaga aataaaatga ggaagcaaca taccttgatc
     1261 gttccgtact tcgaaataac gttgatgttc tttcatagaa gtgactagta cttcttcagg
     1321 aacagataga tatttttctt cgaagtcgcc agaaaagaca gttggatatt cgactagatt
     1381 gttgacttct tctagtaaat cttcatctag atcaatgatc cagtgatttt tttctgctaa
     1441 ttcgtttgct tgtgtgatga tcatttcttt tcttttttgc ggtgaagcaa taacaaattg
     1501 ttcatttaat ttttcttcgt attcttttgc atgcgtaaat gtcacatctt cacctaagaa
     1561 acgatgacct cttgaagctc tgtctgtttt gatatccaag atctcaaacg ggattacttc
     1621 gtcatctaac aatgccacga tccagtgaat agggcggata tattcaaaat cgtattctgc
     1681 ccaatgcatc gttactggga aagtgaggct tttaatgaca tctgctaaac ctgtcaatac
     1741 ttcttttgct tctttgccgt gagtgtattt cgttacatag acatattcca cgccttttaa
     1801 ttctctaaaa gtgatagcat ctgtcgtcag accttgtccg cggacaaatc cttcagctgc
     1861 tttcgtccag tttccatctg cgtccaaagc aatttttttg gctggtcctt tgacttcttc
     1921 ttcactgtct gcttgacgtt ctggaatgtt tgttattttg attgctaagc ggcgcggtgt
     1981 agaaaacgtt tcgatcgtat cataagtcaa ttggttttca tctaaaaatt ttgctgtttt
     2041 ttcttctagt tgtttcatgc ttggtgtgac aacatgagca ggcatttctt ccagcccgat
     2101 ttcaagtaat aagtcttttg ccatcttatt cagcctcctt catgttttct ggatttgctg
     2161 tgtttttatt caataaaggg aatcctaatt tttcgcgttc tgccacaaag attttggcta
     2221 cagcacgagc catgttgcgg atacgggaaa gataacctgc acgctctgtt acggatactg
     2281 caccgcgagc atctaataag ttgaatgtat ggctgcattt caaaatataa tcatacgctg
     2341 gatgaacaag attttcatcg atacagcgtt tagcttcttt ttcaaaacgg tcaaaattgc
     2401 ttagtagcaa gtcttggtcg cttacttcaa aagaatattt ggaatgttca tattctggtt
     2461 gtttgaagat ctcgccatat ttcacaccat cagcccattc cagatcatag acactttcaa
     2521 cttcttgaat atatgaagca agacgttcta gtccataagt aatctcagaa gtaactggtt
     2581 tacaaggtag tcctccgact tgctggaagt acgtgaattg tgtgatctcc atcccatcaa
     2641 gccatacttc ccagcccaca ccggcacaac ccatcgatgg gttctcccag ttgtcttcaa
     2701 caaaacgaat atcgtgttct aatggatcaa ttcctaaaag acgcaagctt tctaaataaa
     2761 gctcttggat attttcaggt gaaggtttca ttacgacttg aaattgatga tgctgataca
     2821 aacggtttgg gttttcgcca taacgcccgt cagctggtcg gcgggatggc tctacataag
     2881 cagcattcca tggttccggt ccgattgccc gtaagaatgt atatgggctc atcgtacctg
     2941 ctcctttttc tgtatcataa gcttgcatca gcatgcagcc gtttgatgac caaaattttt
     3001 gtaatgttaa aatcatatct tggacggtca attttttact catctgtttt gcccctttca
     3061 atttttaagc gttgctgtca ataagaatac aggaaagaaa acgacaaaaa gtccctatgt
     3121 ctgattacag acatagggac gaataatttc gcggttccac cctacttccg aataaagatt
     3181 cggcagcttc atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg gtccggcttc
     3241 cactctcccg aactcgctaa accacgtgtt ttttactgat cttggtcaat gtacgtattc
     3301 attttgcagt tttcttgttt atataataga tcctctcagt gaacgatcta tttaatctac
     3361 ggttaataat agacggaagt ccctgtattt gtcaaatagt tttgattttt ttgttgtatc
     3421 tttcatttat tcttcttttt caggtatctc tttttcaggc atatttttct ctggcgtctt
     3481 taatacgtgt gcccaagact tcatttgatc aatgaacttt ttgcttttta gatgaatacc
     3541 gacatattca tcatacaact ggtcaatgag gtcacggatt gcctgcttcg tttcttcttt
     3601 caagtggatt ccactgatct tatcataaga aatagccgaa aacattcgaa cgaaataaac
     3661 agcgcgagga tctgcatgat aacggtgttc gtccattggc cagtgtttct cgcatatcac
     3721 accgccatat ttggaagaat agtcaaattt tccttttgtt tccccgcata cgacacagtg
     3781 cgtccaaatt ggcgcgattc cgaagcgttg cagtatttga acttcaaaga tattcgtgat
     3841 gatttctgca tcatctcctt gatccatccg ctgcagagct tgttgcgtaa attggaataa
     3901 atgcgggtca tattgatgat cttcgattgc tgcatctact agatttagca aataggtacc
     3961 ataaccgctc aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact
     4021 gcttaaaaac gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg
     4081 gagcaaagcc ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat
     4141 tttgccagaa gattccgtaa agattttgac taatttatct ttttctttgt aattacggct
     4201 agataaaatc aagcctttcg attctgcaag ctggctcata ttctccctcc taaaaaataa
     4261 atctggaaaa gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgccgttttt
     4321 ctcaacgtgc ggatttttat ccagtttatt tctatactta acctataaga agttcagaat
     4381 atcagtattc gtcttttcgg tagccaaagt cttgcagata gacttttttg tctcgccagt
     4441 ctttttggac tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat
     4501 cctttcttgc tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc
     4561 ctttttggct atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt
     4621 ttcgcttcat cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa
     4681 acaagacttt ctcacggatc aattcagaaa cgataaagta ttcaggatga tcagtcactt
     4741 gatcatctgg aaaatattgt gggccctctg gcatttcact gactaataca tccattaagc
     4801 gttcgacatt gtttccttct gtagcagaga tcggaatcac ttgtgcaaaa ggcatctgcg
     4861 tcgaataatc ttcgatgatt cccagcaact cgtctggatg gatcttatcg attttattga
     4921 taatcagata aactggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc
     4981 ggccgcgttt ttgatcagca ctgatcataa ataagactgc atccacttct ttcaacgcac
     5041 tgtaagctgc ttctaccata aaatctccta gacgatgctt tggtttatgg attcctggtg
     5101 tatcgataaa aatcagctga gcttctggtg tagtatatac tccttggatt ttatttctag
     5161 ttgtctgtgc cttgtcactc ataatggcaa ttttttgacc cacgattcgg ttcaataatg
     5221 tggattttcc tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca
     5281 tatacgttct cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa
     5341 cccaatgatg acagcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat
     5401 ttttttccca atcggatgaa aatgaaaatc cgtaaacatg tccactacat tttcaaaaac
     5461 agtattgatg atctctacta tccaaacgag aaaaacagct aaaagcagcc ataaccactc
     5521 tatccgatca agctggaaaa tcacgcccat aaataaagca aggataccga acgcaacatg
     5581 tttcctcata ttgcgttctt cctcaaaaac ggttttgact ccttggacag caaattccac
     5641 agacatcagg aaatgtttgt ttttctctac gtttttatct tttgagtcca taggcatcca
     5701 agatctcttt ctgtaaacca aacatttctt tctcatcttc tggtgtcata tggtcatacc
     5761 cgttgatatg caagaatcca tgtaccgcta aaaagccaag ttcccgatca tatgaatggc
     5821 cgtaagcttc tgcttgttca gcagcacgtt caatagaaat catgatgtct cctagtacac
     5881 gaggcaatgg attctctcca tcttcaaaaa tcactgggat ttcgtcttcg ccttcttctt
     5941 ccagagcaaa actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta
     6001 cttggattgc cgcattgtcc atgaaagtaa cagacatttc tgtttcctct ggcagcttta
     6061 aataagatgc tgcaaattgc agcaagtcat cgatttcttt cattttctct tctgagactt
     6121 ggtttgtttc atccataaag gtaatatcca ttatacatcg cttctttcta ttcttcttgt
     6181 tcttccttta tcttctcaac ttctgttttc attttaggat atttgatccg agaatggaag
     6241 gtgctactta aaccgctgat caatgatttt tccacttttc ggatttcttt caaggtcagt
     6301 ccactatcgt ctaattgacc atcggaaata cgctcttcga taagattatg gacaaaactt
     6361 tcgatttttt cgctcgtcgg atgatccatt gcccgtactg cagcctcaca actgtcagca
     6421 atattgacga tccctgcttc acgactttgc ggttttggtc caggataacg gaattgctct
     6481 tctgttactt ccggattgcg ttctttcgct ttgatgtaga aataacgcat tagcgtggtt
     6541 ccatgatgct gtttgcaaat atcgatcacc atttgcggca tattataatc ttccaatatt
     6601 ttggctccat ctgtcacatg tccaaagata atatgtttac tgtcttctgg caataagaaa
     6661 ttgtgcgggt tttccgctcc tgaaggcaag ttctcaacga aaaagctagc atgtttgatc
     6721 ttgccgatat catgataata gcaagctact cgagttagca aagaacgtcc gccgatttct
     6781 gctacagcgt tagcacttag attagctacc atcatactat gatgataagt acctggcgct
     6841 tcttccagca actgtttcaa taaaggatga tttggattgc tgagttcatt taatacaatt
     6901 acactgtcat cattgaccat caattcaata tatgggtgta gaccgatcgt cagcaagaat
     6961 gagaagagtg caccagtgaa cccgcagaca agcattaacc aagtcgttgc atcaccaaag
     7021 ctcattcctt gatagacgat caaaatgaca tccatcatca caggaaaaat aatgacccac
     7081 atggcagcag ataatccttg ttctgagatc cgtttgcgtc tgacaatcgt tgccatgagc
     7141 ccagagaaaa cataagacat caagataatc gttagaaagt tcgtgccaat cgaatcataa
     7201 aaaatgaaaa gtgcagatac tgcttggaaa ataccagcca tgatccctgc tcttcggttc
     7261 aagaaaaagc ttaaaacaag tggtgcaaaa gcagcgggat aaaacagcgg gatataagta
     7321 gcttgttccg tttggaataa ttggaaaaat ttcatcagca ctatgctgac tgacatagct
     7381 gtcacataga aaagcacgta ttttgttctt tctcctgcat tttggaattg aagtgaatta
     7441 taaatcagta cgccgatttg aagcaaaaca gctaaaacca tggcaattag cggaaaaatc
     7501 gaagttgttt gattagtcag tcctagtaat tccaattttt tcatagctga agcatcgatt
     7561 tgattacctt cacgaacaat gatttctcct tggaagatca tcactggttg tacagcttct
     7621 cgtgcggatt gtttcaattc ttctgtcttt ttctcattca atacgtcatt cactacgata
     7681 ccttgatcca ctaagtagcg aatcatttgt tgttgtgctg gtgtgacatt caaatattgg
     7741 atttgctctt ctgcttcttg tttaaaagct tccaattcac tttcacgaac ttgtttgctc
     7801 atctgttcgt caatgagctt cagactctca tccctcacag tatggatctc agtttcagtc
     7861 atttcaaata tcgtcgaata aaaattgttc ggtagttttt gataaaacgc aacattttct
     7921 gcattcacat tttcaaaatt tttcttcaac gcggcgatcc gctcttctgt agtaggcttt
     7981 gggatatttt catcttcctt tgccttatca atcttttcgt cataagattt gttgatggca
     8041 tcattcgttt tatcgatcaa ttcaaatagt tgcttgatcc gattgttctg gtcatctgca
     8101 agatcttttt ggtaagtgta ttctggagtg actgcttctg ctgccagttt tcttttttgt
     8161 tcggtttctt ccgtgttctc gatcgttttg ttcgcacgga tactttcttc tgctacttgg
     8221 ccttctctga aatcattggc cttttgtttg acactactaa atgtaacgcc gcagataatg
     8281 ataaaaaaca agaacagcag gaaaggcaat gctgcctttc caagtttttc ttgtagtttc
     8341 tgcagtgttt tattcaacat gtgcttatcc tcctaagcaa agcttattct tgatggagat
     8401 ctgccttttc ataagctttg atgatttcgg caactacagg gtgccgaaca acgtcacttg
     8461 cttcaaaatt aacaaaatcg attttcttga tttgctttaa tgttcgttct gcatgaacaa
     8521 gaccacttgt tgtccctcga ggcagatcaa tctgactcgt atctccattg acaatcattt
     8581 ttgattgata accaagacgt gtcaggaaca ttttcatctg agcaactgtc gtgttctgtg
     8641 cttcatccaa aataacgaat gcatcatcca atgtccgccc ccgcatgtaa gctaaaggag
     8701 caatttcgat cacgcctcgt tccatcaatc ggttcgtatg atctaatcca aaaatttggt
     8761 ataaagcatc ataaacagga cgcagataag gatctacttt ttcttttaga tccccgggaa
     8821 gaaagcctag attttcccct gcttccacag caggccgcgt caaaatgatt ttttgcactt
     8881 cacctttttt caaggcagcg attgctaata ctactgcaag aaaagttttt cctgtcccgg
     8941 ctggtcctac gccaaatacg atatcatgtt tggctaccga ttcgacatat ttcttttggc
     9001 cgctgttttt tacccgtatt ggttttccat tacggtcttt gacgatctct tcctcataca
     9061 tttcaacaaa ataatctaat gtgccttttt gccccatttt cagtgccgtc acgacatctg
     9121 gtgtacttac atggattcct cgtttgatca attcctgcaa tgtccgaatg actgaggcag
     9181 ccaaggaaat ctgggattgt tcgccaatga tctgaatgat ttctccacgt gtatggatgg
     9241 ttgtttctgt tgtttcttca atcagtttta tatgcttgtc gtgtgcacct aaaagcatgc
     9301 tgacatcatc agcagccgtc aatctaatct ctaaagaact agactcttcc gtcaaaaata
     9361 gacatcccct ttttttcgat tcattctttc ctattttacc atactcagaa gaaactatat
     9421 agcaaataag aaaaggttct aaaagagttg cttaaccata agtaccaaga aacgttaaaa
     9481 aatagctgct tccattctct aaaggtctcg tttgaataaa aaaagctgat catgtaagct
     9541 atgcataaga tagaggacaa gtattccgtc taacttagca agtattctct gggtattttt
     9601 cttgtttcaa aaaaagaaaa caaaatattt gaaatattct tgcatttcaa aaaaaacagg
     9661 tgtattataa cgtcaacaaa taaatataca gcaaagcgtc gaggaaaaca gtaggctatt
     9721 cagcgattta ttcccagtga gtcatgtaag tgagaaatga caataaatcg aatagacgaa
     9781 catcatttcc gagtcaatgt cgaaaacgta gatcattgtg gctgcctcca ttatcaggct
     9841 agcggataac tttatcgaaa agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg
     9901 tggtaccgtt ctagaatcta gagccctttt cccaagtgga aaagggcttt tttttatatt
     9961 ttaaggtggt ggttttcatg gaatctgata ttagtggcat ttctagtttc tgcaacgttt
    10021 attttgttac tcaaaatgat aattattgga ggaaaaattt atggaagaaa caaaaactta
    10081 ttcatggaag caattattga tggtcagttc acttatcttc gggatgttct tcgggtcagg
    10141 taatctgatc tttcctgtcc acttaggtca aatggctgga tcaaactgga tcagcgctgg
    10201 tgtaggattt gccatctcag gcgctctttt cccattatta gctatcttag ctgtagtagt
    10261 gacaaaaagt gatggtctgt atgacttagc taaacctgtc ggaaaatggt atgctgcctt
    10321 gttcttgatc cttgttcatt taacgatcgg accattcttt ggtacaccgc ggacagcagc
    10381 aactgctttt gaaatggctg ctaaaccctt tttgcctgaa aaatatgatt cattaggtat
    10441 gtttttattc tctgctcttt tctttttact tgcttatttc cttactgtac atccaacacg
    10501 tttggtcaaa tatgtcggaa agtatttaaa ctccttgttt cttgcattat tagctatcat
    10561 ttttgtcgtt tctttattcc aacccatggg gaatctaaac caatctgctg cagattctta
    10621 tcagacgaac gctttgacaa atggcgtttt agaaggatac aacacggttg atgcagtagc
    10681 tttacttgct ttaagtgtca cttttgtcca tgcagttcgt gatcttggtt ttaaagataa
    10741 aaaagtcaca gaattaacag caaaagctgg tagcctgagc gttgcgttag aagtcatgat
    10801 ttattttggt ctggtccttc tcggtgcgat gagtttgaat aagatgcctt tatcagaaaa
    10861 tggcggaact gcactttctc agatcactaa tttttattta ggtaacttcg gtgctagctt
    10921 cttaggggta atggtcacat taggtgtctt cactaccgct atgggactcg ttgtttcttt
    10981 tgcacaagat ttccataaac tgttccctaa agtcagttat atgacatggc tgcggttaac
    11041 gacttttgtc tcttttgtcg ttgcgaatgc cggattggat aatatcatcc aatggtcttt
    11101 accagtattg atgcttttgt atccattgtc acttgcgtta atcttgcttt ccttaacagc
    11161 aaaattcttc caaaagagtc catttgtcta tcaagtaacg atgttctttt ctgccgttcc
    11221 agccgtattg gatatgttgg caaattcgcc tgcattagtg agtcagcaaa ctgtcgtagc
    11281 aagcttacta ggattttatc atcatcacgt tccttttgct gcattaggac ttggctggat
    11341 ggtcccaacg ttacttggtt acgctggcag cttactcttt tattacgcct atcgcctatc
    11401 agggtataaa caagaagcaa atgaactacc agaagaataa ccaaatcaaa cacaatttaa
    11461 cataagagat attaaacagg
//
LOCUS       DAJSVF010000081        11241 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSVF010000081 DAJSVF010000000
VERSION     DAJSVF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.084"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(592..936)
                     /locus_tag="ON550_002181"
     CDS             complement(592..936)
                     /locus_tag="ON550_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955252.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1033..1278)
                     /locus_tag="ON550_002182"
     CDS             complement(1033..1278)
                     /locus_tag="ON550_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955253.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1348..1602)
                     /locus_tag="ON550_002183"
     CDS             complement(1348..1602)
                     /locus_tag="ON550_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955254.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1766..2089)
                     /locus_tag="ON550_002184"
     CDS             complement(1766..2089)
                     /locus_tag="ON550_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955255.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2276..2349
                     /locus_tag="ON550_002185"
     tRNA            2276..2349
                     /locus_tag="ON550_002185"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Ile,seq:cat)
     gene            complement(2734..3231)
                     /locus_tag="ON550_002186"
     CDS             complement(2734..3231)
                     /locus_tag="ON550_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3955256.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3271..4080)
                     /locus_tag="ON550_002187"
     CDS             complement(3271..4080)
                     /locus_tag="ON550_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3955257.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4414..5271
                     /locus_tag="ON550_002188"
     CDS             4414..5271
                     /locus_tag="ON550_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3955258.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5264..6166
                     /locus_tag="ON550_002189"
     CDS             5264..6166
                     /locus_tag="ON550_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3955259.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6199..6549
                     /locus_tag="ON550_002190"
     CDS             6199..6549
                     /locus_tag="ON550_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3955260.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6598..7521
                     /locus_tag="ON550_002191"
     CDS             6598..7521
                     /locus_tag="ON550_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3955261.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7599..8492
                     /locus_tag="ON550_002192"
     CDS             7599..8492
                     /locus_tag="ON550_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3955262.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8530..9093
                     /gene="amaP"
                     /locus_tag="ON550_002193"
     CDS             8530..9093
                     /gene="amaP"
                     /locus_tag="ON550_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3955263.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9107..9295
                     /locus_tag="ON550_002194"
     CDS             9107..9295
                     /locus_tag="ON550_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3955264.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9308..10210
                     /locus_tag="ON550_002195"
     CDS             9308..10210
                     /locus_tag="ON550_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3955265.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10242..10484
                     /locus_tag="ON550_002196"
     CDS             10242..10484
                     /locus_tag="ON550_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3955266.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10740..11111)
                     /locus_tag="ON550_002197"
     CDS             complement(10740..11111)
                     /locus_tag="ON550_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955267.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3980 a         1925 c         2129 g         3207 t
ORIGIN      
        1 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
       61 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      121 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      181 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      241 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      301 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      361 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      421 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
      481 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
      541 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
      601 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
      661 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
      721 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
      781 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
      841 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
      901 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcacataga
      961 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1021 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1081 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1141 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1201 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1261 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1321 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1381 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1441 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     1501 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     1561 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     1621 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     1681 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     1741 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     1801 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     1861 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     1921 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     1981 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2041 ctaaatcgaa taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat
     2101 aaaagaataa cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg
     2161 acacaagtaa aagaaataag aataacaata ttattttact tatattttga aacttgtgag
     2221 agtttgtcat tcgtgctata gttgaaaagt aacaaaaata aattttattt atgatgtacc
     2281 tttagctcag ttggttagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta
     2341 caaggtacat aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     2401 aaaaacagct tcttatgttt ttacttggct gttcttatta ctgcagatgc aagcttttga
     2461 ataccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa
     2521 cggaaaaata gttcctcata ttttgccgtt tgttaagcta ataccgaggg tcagctgatt
     2581 gaacaccatt taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc
     2641 aatctacttt gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct ttttttattg
     2701 tgtgttaaat atcgtaaatg aagatagata tttttatgat tcgacgatag agacgttgaa
     2761 atctttcaac agctctaaag cggattctcc gtgttttcta ctggagacta acattggatc
     2821 aacaaaaaag tctgcttcag gaaaagcact ctgcaggcct atcagattag agagtacaca
     2881 caagtttgtt tcaatcccga ctacttcgat ctctttttgt tcttctttgc tttcaaataa
     2941 agtttcctgg atttcagcta aagtttcagg tggaatcgta aagtaatact ttttaatcac
     3001 tcgttcattt tcttgtggag aaagaagagg gatcaattgc aaatcttctc gttcttcttc
     3061 tttgtccttc agttcaactg gtatatcccg tgtgaagaga atatattcat tattttggcg
     3121 tgctttagct aatctttgag caatacgttc tgccaattga tctgactctg gaatataaaa
     3181 ttcgctgttt ggatcaagaa tatgattttg catatcaata ataactaaca ttttctttcc
     3241 tcctacattc atatcgtcat tttattcttt ttaatagaag ttttttaaaa tataagcaac
     3301 accagcttct tcattcgtca gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc
     3361 attccccatc gcaatactca acccgctgta attcaacata tcaatgtcgt tttcttgatc
     3421 gccaaaagcc atgatgtcac tcgattcgat tccaaggtaa tccgccagaa aaacaagtga
     3481 atgagcttta gtgatacctg taggcgtgat ttctaagaaa tgcggatcag agaatactga
     3541 ttggatacca tctgcttgca gatctctttg tagcttctgt agtttttctt cactcttatc
     3601 tgttaatgct aatttaagat agctaagaga aggatcttcc aacatattta aaatttgatc
     3661 aaaagaatgg ttctgtaaat tgaaatgttc ataataaggg tcatctttga ctagatttga
     3721 ttcatcgaat gtcagctgat catcttgggt ttctcggcta gcaatgatgg atattttcgc
     3781 ttcttgagca tagaagaagg cttttctcag tgtatcgcta tctatttttg aacgtctgag
     3841 aactttcgcc atttttccat ccttcatttc agcaatcagt gaaccattgg aagaaatcaa
     3901 atagccaccg attcctaatt cggaaaaaat atctttagca gaaatgctgt tccttccggt
     3961 agcgacggtt acgattccgc cattttcatc aaatttccga agtgcttgga tattttcttc
     4021 tggtatcttt ccatttcgat caagcaaggt accgtctaaa tcaatcgctg ctagtttcat
     4081 tttatcttcc tactttcttt ttacatatct attttcctgt taccttaaga ttaaatgaga
     4141 atttaaaaaa atgaaactaa cttgctttgt cattatgaat tttttcaaaa aacattctta
     4201 agaaaaaggt ggctatatat agtagttttt ggtcaatacc tttgaataaa aagccatgaa
     4261 aaacgaatag ttagcgctta ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat
     4321 gaaaacaaag catatatttt gctttaaaac caaatgagga atattctaga tgtagaacag
     4381 gttagaaaaa gattagaaaa aggagtgatt ttgatggaga agagatacgg cgtagttcct
     4441 gtggtaactg cttcagacga aaattatgct ccttacttga gcgtgatgat cgcaactgca
     4501 ttagaaaatt gcaataaaac aagacgcata aaattttatg tgattgacga tggtctatca
     4561 gaatatagta aagaaggatt ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc
     4621 caattcctga cagtggaaaa agatatctac gaagacttcc tagtcagtga ccatatcaca
     4681 actaccgctt acttaagaat ttctttacca aacttattag ctaaagagga ttataagaaa
     4741 gtcctttact tagattcaga tgtgttagta ctagacgata tcgtaaaact atatgatgag
     4801 ccgttaaatg gaaaaacaat cggcgctatt atcgatccag gccaagtgaa agctttggaa
     4861 agattaggaa tcgattccga tgacttgtac ttcaattcag gagtgatggt gattgacatt
     4921 gatcaatgga ataaaaaaga aattacagaa aaaactatcc attacctcag tgaaaatggc
     4981 gatcgaatca tctatcacga ccaagatgct ttgaatgctg tactttatga agattgggaa
     5041 caacttcatc caaaatggaa catgcagact tcgctgattt ttgaacgtca tccagcacca
     5101 aatgaaaaat atgaacgtca gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt
     5161 acaggacatg ataaaccttg gaatacgttg aaagatcatc cgtataccaa tttatactta
     5221 aaaaaattag cacacagtac tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat
     5281 agcagtagtc gcaagttgta acacaaaatt cgtaccacat ttagctgcat tatttgtctc
     5341 agtattagat aattgcaacc cgtctaaatt tgttcggttc tatgtgattg acgatgatat
     5401 cgattttgaa agtaaacagc ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga
     5461 tgtggaattc ttgaaaatca ataaagaatt ctttacaaat gtggtaatca gcgatcgtat
     5521 tccagaaaca gcttattatc gtattgcgat tccggaattg ttccgcggca cagaagttga
     5581 acgtatctta tatatggatt gcgacatgat cgctttacaa gatatctcta aattatggcg
     5641 tcttgacttc ggtgattcaa tcgtagcagc tgttgaagac gctgggttcc atcaacgact
     5701 ggaaaaaatg aaaatttcag ctaaatcaat gcgttatttc aactcaggtt tgatgctgat
     5761 caatgtgaaa aaatggttag atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca
     5821 taatccagaa aaattacgat tccatgacca agatgcatta aatgctatct tacatgaccg
     5881 ttggttacca cttcatccgc gttggaatgc acaaggctat atcatggcaa aagcaaaaaa
     5941 acatccaaca gcagcaggtg aacgtgaata cgaagaaact cgcaacaatc cttacattat
     6001 ccatttttca ggacatgtga aaccgtggag caaggatttt gaagggccaa cgaagaaata
     6061 ttacgaaaaa tatgcaggga tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc
     6121 aaaatatgca aaagttcaac caaaaacaga tgtagttgtt aaataataga agacaacaac
     6181 tgaagagggg gagtaggaat ggaaattaca atgtctaaaa atgcagtaga gacgttaatt
     6241 gaaaaggttg gggaaaatac aaaaatcgca ctcgcgctta tcaatgattc cgatccattt
     6301 ctaagggaca aaggtgcttt tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg
     6361 gaatttggag aatatgcgac taaaatcgag catccattat tggacatcta tacatctaaa
     6421 ttagagcaaa attatttcgg taaacgattg aacatggact tcaacaaaca gcttgacagc
     6481 ttttcattag aaaatgaaat cgcagtactc gattataata tcaaattaaa gaactgcttc
     6541 ttcagctaat agtttcgaga aagtccaaaa aggaggtata cattgagata ctactttgtg
     6601 gatgaagaaa agaatgtaat agaaacagat gaaaaaaaat ataattggct cgtacttgat
     6661 agtgcaaagc ccgaagagat tgagcaagca atcgaagaat tcgagcttcc tgatgatatt
     6721 ttcgtaggaa ccgaatatcc ggaagaagtt tctcggatgg agcgccttta tgagacgaaa
     6781 ctagagcatc cattttcttt ggtagtgatt aatctagatc agtcagataa tcgtatcgaa
     6841 aaaaaattaa cgccaatatc ttttgtactt tccgataatc tcttgattac ttgtatcgat
     6901 agaaaaaccg aatttgtgga tcggctgctg acaaagcatg gaaatcattt aaactcgttt
     6961 gaaaagatca tcacgtatac attattagat atttacaccc attacgtgaa agaactaaga
     7021 gagatgaaaa agcgaattga cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa
     7081 gaactgtata aacaggcaga tcttgaaaga gacatcgtat atatcgatca taccttaaga
     7141 gatcaacggg aaacaatgga tcaattatgg aattctgaaa ctttcatcaa acgtttagac
     7201 gatgaacagc tactctatga tgtccatttg agacagcgac aaacagaaaa aatgatcgag
     7261 atctatcgag atttattaga gagtattggt gatctattca acgggatgat ggacaataat
     7321 ctaaatcata tcatgaaata tctagattca gctgcattaa tcatttccgt tccggcgttg
     7381 ttcgcaggga tttggggaat gaatacagga ggattaccgg gtaaaagttc tgctctaggc
     7441 tttatactcg tagtctttgg ctctttgata gcagcagtag ttttaggtta ccatttatac
     7501 cggaaagatt atacaaatta aagaatctat ttaaaacagg tatcaaacaa agaaatttgt
     7561 tttggcaata caatttaaaa actaggagga atacagcaat gacagaacca aaattaatcg
     7621 acccgcgaaa cttgtaccat acggagaaat ttcctaaaca agatcaggat acaccagctt
     7681 tacagcagaa tatgattcct gttcctgatt gtggagaaga atcctatgaa ggcaataatc
     7741 aactagaaaa ccgccgtgta ctaatcactg gtggagattc agggatcggt cgtgcagcag
     7801 cgattgcatt tgctagagaa ggggcagata tcgctcttca attcttccca ggagaagaaa
     7861 aagatgcaga ggaagtagca aaatacgtga aagttgcagg acgtaagatt gtcttactac
     7921 cttttgatct tcgtgacgaa caagcaccta aagaaatcat agataaagcc gtgagtgaac
     7981 ttggcgggct agatacgatg gtactaaacg caggacagca aatttcttgt cagtctattg
     8041 ctgagttacc acttgagcaa gtcaaagaca catttatgat aaacatcatc gctatgtttg
     8101 ctttagtgaa agaagctgtt ccgcatatcc cagcaggagg agcaatcgta acaacaactt
     8161 ctgttcaagc attcaatcca agcgaacatt tattagatta cgcagcaaca aaagcttcaa
     8221 tcgctaactt tacgatcggt ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg
     8281 gtgtagcacc tggtccaatc tggacaccat tacaactaga cgaaggacaa ccttctgatt
     8341 cgatccctga atttggtcag caaagtttac tagaacgtgc aggtcagcca ggagaattag
     8401 caccagttta tgttttctta gcatcgaata aagcaagtta tgttacagcc caaatttacg
     8461 gagtaactgg tggagaagcg atcaacttat aagaagttga tcgtttcacc cctatgagga
     8521 ggtgcgaaaa tgaataaagg tgtaaaaacg atcggagtga tcggaacatt acttttgcta
     8581 tctgtgcttt tgtttgcgat actattaagt gcaacctatc cattgccttt tcagctagag
     8641 aagtttcgat tcttttccat cactaattca tacgtgcaac aatatgtatt ttggcttgcc
     8701 gttgcatttg ctattttgac aattatcacg ttacttgttt tcctgtttta tccaaaaaca
     8761 atcggaaact ttctactgaa acatggtgat ggggagttga cgttggataa aaaagcaatt
     8821 gaaggaatgg ttcgttctca cttgcatgaa gatgaattca tccattcacc aaaagtaaat
     8881 gtcaaagcaa cgaaaaacaa gatcgccgtc aatgtaaaag gggagttgaa acgaacttca
     8941 gcactcattg gtaaaacagg aacattgatg aaagaaatcg aaaatgaagt acaacacata
     9001 ttgggaacaa aagagccagt gaaagtatcg gtgaaataca caggctttga agaaccagct
     9061 aaacgaagca atgaccgcaa acgagtagaa taggggggga ccaaaaatga aagaactatt
     9121 ttcgacatac aagctcccta tcatctttgg ggtacttgga ttgatattag ccgttttatg
     9181 gatcaccgtg ggctttttca agaccttatt gatcttgatc atggcagcga tcggcgtcgc
     9241 tatcggtttt tatctgaaac aaacgaaaat cttagatcac tattttaaat cttaggagga
     9301 aacatccatg aataacaata acaaaaataa taacaataaa atgaacaacc aaaaaccagt
     9361 gtctgcacat ccagacccaa cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa
     9421 caaaacagca ccagtatctg cacatccaga tccagtagtc cctaacgttc aacaaggagt
     9481 agaagagaag aaacatgatg cagaaaagca tgcacctcat catccaggaa ctccagaatc
     9541 taaagttcca gaacaacatg atgcaatcga tccttctaaa cctgtggaag agcaaaaaca
     9601 tccttctgac catgcgaaag cacaagaaaa acttagttct gaccacacaa acggacaagg
     9661 aaaacttggt tctgatcata caaataacca aggaaagcat gattctgaac aggaagattc
     9721 aatgcgcggt gagctgaatt atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat
     9781 tgaacaagta gacggtttat tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa
     9841 acttgtgaat acagataatg taacggctgg gatcgaaaca gaagtcggta aaaaacaagt
     9901 agcagttgat atggatgtca ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga
     9961 aatgcaagaa gtaatcggca aagaagtaaa aaatatgaca catttagaag tgatcgaagt
    10021 caacgctaac gtagtggaca tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt
    10081 gcaagacaaa gtaactgacg cagcgaaaaa aaccggtgaa tttgcttcga atcaaacaga
    10141 taaagtaaaa tcagcagctg gaagcggcgc acaaaaagta aaagaaaata cagaaccacg
    10201 ggttcagtaa taaggaagta aaacattagg aggaacgaaa catgggattt atttggtcac
    10261 ttatcgtcgg aggaattatc ggagcaattg caggagcaat cacgaataaa ggttcttcga
    10321 tggggattat tgcaaatgtc gtagcaggtt tagttggatc agcacttggt caagcattat
    10381 taggttcttg gggaccaagt attgcaggaa tggcaatcgt gccatcattg attggtgcca
    10441 tcattttagt actaatcgta tcattctttg ctcgaaaact gtaaaaagac ttttaaaaag
    10501 aggtcgtgaa aaagctgttc ttcatcaagt aataagaaca gccaaacaaa aatagcttct
    10561 catatttttc atttggttgg gcatattatc gaagatgcaa gcttttgaac actgtttata
    10621 cggaataaga ggctgtgaca agacttttgt cacagcctct tatttatgcg ggcaaattcc
    10681 caattagatt tttaaaagtt tttccttaaa tgtgtcttta ttgatacaga gaagcttctt
    10741 taataaaaca atgcctccct aaattttctt gagataggaa aggtgaacat acaaaataat
    10801 acgaccactt tgaaaaccca gatgatccac aaatggatcg tatgatactg atgaagtaaa
    10861 aagcaatgga tcagaaactc aaagagattg aaaaagacaa ggaagaaata atatctcttt
    10921 tttactcttg tctgttttag ccaataatag ctcgtaaagt aatctagaca aacaaataga
    10981 agtaaacaaa gtttcgtaag agcagcaatc gtatcatgtt gctctgctag acgattgacg
    11041 agcaggaaaa atgttggtaa tggaatatag atcacgagta atgaaagaaa aaagtaaaca
    11101 ataaatggca tactattcct cctaattgtg tgagttcgcc ctcattttaa aataacgata
    11161 tttgtttgtg aagctttttc gtgtcgtaac aaatgttatc taatgtattt ttaaataaat
    11221 aatcattatt atcaaaataa t
//
LOCUS       DAJSVF010000082        10798 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSVF010000082 DAJSVF010000000
VERSION     DAJSVF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.085"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            9..527
                     /locus_tag="ON550_002198"
     CDS             9..527
                     /locus_tag="ON550_002198"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3955268.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            655..1338
                     /locus_tag="ON550_002199"
     CDS             655..1338
                     /locus_tag="ON550_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR3955269.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1647..1720
                     /locus_tag="ON550_002200"
     tRNA            1647..1720
                     /locus_tag="ON550_002200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1681..1683,aa:Arg,seq:tct)
     gene            1879..2238
                     /locus_tag="ON550_002201"
     CDS             1879..2238
                     /locus_tag="ON550_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR3955270.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2361..3992
                     /locus_tag="ON550_002202"
     CDS             2361..3992
                     /locus_tag="ON550_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR3955271.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4054..4695)
                     /locus_tag="ON550_002203"
     CDS             complement(4054..4695)
                     /locus_tag="ON550_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3955272.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4881..4957
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4977..5285
                     /gene="rplU"
                     /locus_tag="ON550_002204"
     CDS             4977..5285
                     /gene="rplU"
                     /locus_tag="ON550_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR3955273.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5312..5656
                     /locus_tag="ON550_002205"
     CDS             5312..5656
                     /locus_tag="ON550_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR3955274.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5669..5962
                     /gene="rpmA"
                     /locus_tag="ON550_002206"
     CDS             5669..5962
                     /gene="rpmA"
                     /locus_tag="ON550_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR3955275.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6055..7203)
                     /locus_tag="ON550_002207"
     CDS             complement(6055..7203)
                     /locus_tag="ON550_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3955276.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7215..7526)
                     /locus_tag="ON550_002208"
     CDS             complement(7215..7526)
                     /locus_tag="ON550_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955277.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7579..7974)
                     /locus_tag="ON550_002209"
     CDS             complement(7579..7974)
                     /locus_tag="ON550_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3955278.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8010..8354)
                     /locus_tag="ON550_002210"
     CDS             complement(8010..8354)
                     /locus_tag="ON550_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955279.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8655..8876
                     /locus_tag="ON550_002211"
     CDS             8655..8876
                     /locus_tag="ON550_002211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955280.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9003..9779
                     /locus_tag="ON550_002212"
     CDS             9003..9779
                     /locus_tag="ON550_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3955281.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9782..9970
                     /locus_tag="ON550_002213"
     CDS             9782..9970
                     /locus_tag="ON550_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955282.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            9972..10256
                     /locus_tag="ON550_002214"
     CDS             9972..10256
                     /locus_tag="ON550_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955283.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10277..10465
                     /locus_tag="ON550_002215"
     CDS             10277..10465
                     /locus_tag="ON550_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955284.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
BASE COUNT         3444 a         1777 c         2076 g         3501 t
ORIGIN      
        1 tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg ttgattatat
       61 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
      121 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
      181 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
      241 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
      301 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
      361 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga
      421 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
      481 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
      541 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
      601 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
      661 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
      721 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
      781 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
      841 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
      901 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
      961 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
     1021 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
     1081 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
     1141 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
     1201 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
     1261 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
     1321 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
     1381 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
     1441 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
     1501 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat
     1561 aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta
     1621 taattattga tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct
     1681 tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact
     1741 gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa
     1801 taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc
     1861 agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt
     1921 gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg
     1981 gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac
     2041 caaggagtgt acaatggact attaggagtg ggactacttt atggagctta tctttcttcc
     2101 gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc
     2161 agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg
     2221 ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga
     2281 aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc
     2341 atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt
     2401 caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg
     2461 tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt
     2521 tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa
     2581 tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa
     2641 tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt
     2701 tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag
     2761 gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag
     2821 gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag
     2881 cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg
     2941 cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt
     3001 tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt
     3061 tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca
     3121 ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa
     3181 ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc
     3241 ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat
     3301 ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc
     3361 ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg
     3421 tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt
     3481 ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta
     3541 ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta
     3601 tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc
     3661 tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac
     3721 tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg
     3781 taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc
     3841 aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc
     3901 acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt
     3961 tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt
     4021 aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag
     4081 tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg
     4141 atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc
     4201 tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc
     4261 tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac
     4321 cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt
     4381 ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg
     4441 atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga
     4501 agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat
     4561 ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc
     4621 agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc
     4681 ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca
     4741 atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt
     4801 tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag
     4861 tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca
     4921 tatgctgtca cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt
     4981 acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg
     5041 ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag
     5101 gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg
     5161 ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt
     5221 cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg
     5281 cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga
     5341 cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag
     5401 tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc
     5461 attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac
     5521 cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa
     5581 tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac
     5641 aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc
     5701 ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg
     5761 tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg
     5821 tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa
     5881 agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta
     5941 tccagtagct caagaagctt aataaataaa ctgctctatc tcttgtgtag caagggatgg
     6001 agcttttttg taaattatgg gatttttttt gaccatactt ttgaccatac tttcttagaa
     6061 gttaatataa tttgttagtt tttgtgttgt ttcttctttt ttgctgttag taatgtgtgt
     6121 atatgtgtcc atagtagttt ttatatttgc gtggcccatt ctttcttgga cttctttgtg
     6181 atctgcgcct gcctcatata gcattgatgc atgtgtatgt ctaaaaccat gcagaccgat
     6241 ataaggtaag ccggcacgct cacgatatat attgtatctt tctgccactg actgattaat
     6301 tgtgaaatta ttttcgtcat tggtaaacaa ataattagct ttagtaaatc ctaattgaaa
     6361 taagtacttt ctttgttcga gcttccaaaa ttttagttgc tttatagttt tttcatctaa
     6421 ataaattatt cgatttgatt ttttggtttt aggagtttca gatatataat atttatctga
     6481 acgagctaca gtttttttaa tggccatttc atttgtttta aaattaatat tgtcccaagt
     6541 gagtgctaat atttcgccga ttctacatcc gctgaatgct aatagtctaa agagcgtata
     6601 gtctctgttt ttaaaatatg gattctgttc ttgagatact tcatttagaa ataattccaa
     6661 ctgttctttt gtatataatt ttaatttctt ttctgtttta atttgtggct ttggaataag
     6721 taaatttaat gttggattat cagatgttaa accaatattt atagcatact taaaaacttt
     6781 attcatatag tttatgaaaa gagggtactg ctttgaagtt cctttttcac tccaatcatt
     6841 tactaccttt tgacaatatg ccgtattaat tttagaaagc ttaatatttc caaatttggg
     6901 atatatatgt ttattaaaga ttattttagt ccttgaatat gaactttctt ttacagtttt
     6961 tttgtagctt tctagccata attctgctgc ttctttgaaa gtagtatttg tacttgtggg
     7021 cattcctgtt ttttgtaatt ctagttctaa cctagaaagt gctgtttttg cttctttttg
     7081 tgttctaaat cctcgtttag tagtatattt tttctttcca gttagcggat caataccgag
     7141 ataagtttta aagtaccatg ctttttcgcc atttttcttt ttatattgtt ttatcattgc
     7201 catcaatatt acccctattc aaattctaat tctgccatgc tatcattgaa aaattcgatc
     7261 agtccttgtt cctctataaa ttcaagccat atttccttcc cgttaattaa tggaactcct
     7321 ttttctttac gaattgtttg atacaagtta gtcacgactt gtttccacat ttctaataca
     7381 gctttatcta ttttcttatc atcccagcgt tttttttggg gtctattttt gtttttttca
     7441 atttgataat tagtagcttt gatgataaat gtttcgtatc cggttgtgct tttttctaac
     7501 cattctttga attctgtata tatcattttt gttcaccttt ctatttttga acgtatgttc
     7561 ttttttgctt aaaaaatttt agagagctgc ataacaatag atgcgagctt ttaataattc
     7621 tttcacaagt ggttcgaagc gaacgcttaa attgttatct tctataaatt tcatgtaatt
     7681 gatattgtgt ggttctaatt cagactttgc aatatatcta tctaataatt tttcaatcat
     7741 gaagcgatca gcctcatatt ccatttttga atggagagag aatgctaagt tatataaaaa
     7801 ataattatca tgatgtttag aagcgtgtcc taattcatgt aataaggttc tttgtcttct
     7861 atacttagat aaattagctt ttattactat ggtattcata caagcaacat aatggccatc
     7921 ggcatcaagg ttttcacgct cttctacctt aacgccgagt tcattaatta tcatttcaat
     7981 ttgactgtcc aaatgactca cctacttatt tagttatctt ttttctctaa atatgcttca
     8041 atcatagctg atagtatttc cctatcatta tcggtgagcg gttttccgtc gctactcata
     8101 acagatgcta aagcttcttc cacggttagt tttctttcct ctggtgcaac tcccgcgtta
     8161 ggatcatctg tgcgtcctag caaataatct gttgatacat ggaagtagtc agcaactttt
     8221 tgcaaagttt ctattttagg actttgtgtc ttccatttat aaatagagtt ttttccaaat
     8281 cctattctat tttctaattc agaaatagaa atacctcttt ttttacagag ctctttaacc
     8341 ctttcgaaaa gcatgtcaaa tcaaccttcc tagagcgtac gataataatt tatacaaaaa
     8401 gatagaaagg tagttgacaa atctatccaa ttgtcttata tttaactcgt aagctaattt
     8461 aataagctaa caagcaacaa gaaatacacc taataaaata aacgaattcg aggtcggcaa
     8521 actaagaatg ttaattttta gggtttttct aggcttattt aactgtatat aaatataaga
     8581 cagttggata gaaatgtcaa taaaaattag ctaattttat tagcttacat atttaaagaa
     8641 aaggaggaaa catcatgaaa caaaataaaa aaactccttc cgcaaaagga gctttaataa
     8701 ctttaaattt taaagaccaa aagtttagtg ctgttgttga tgaaattcat ccacaagagc
     8761 atcaaaaaaa ttcttttgca actgacgaga aaaagactca gaaacgctct gagaaagttc
     8821 ttcgaataat tcaatttctg ttggaaaaga ttcgtcattt tggaagtcta ttttgaattc
     8881 tttattaaca gtcttgaata cggtttgttt gaataaggcg tcgacatcca tattaaattc
     8941 ttccatacta ttttcacctc gctttctatt caaaattata tcaaagaaag gaatgagcaa
     9001 aaatgaacac accgcaaatt tttaactttg aacaacatga agtaagaaca gtaacaatac
     9061 atgatgaacc attttttgtt ggaaaagatg tggctaaagt tctagggtac caaaatggta
     9121 gtagagatat taacagacac gtcgatgtag aagatcggca aaactaccaa aacggtactt
     9181 ttgaatcgcc tagagggtta actattatta acgaatcagg tttgtactcc ttaattttag
     9241 gaagcaaaca accaaatgct aagaaattta aacgttgggt gactagcgaa gtgttgccag
     9301 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
     9361 cagatacaat catccaatta gcaactaagt tgaaagaaga acgtactgga agactaatcg
     9421 cagaacaaaa aattgcagaa tacgaaccca aaatttcgta tttggatagt atcttatctt
     9481 ctacagattc agtaactatt agtcaaatcg cggctgatta tgggatgtct ccacaacaga
     9541 tgaacaaact acttcataag ctaggtattc agaaaaaagt aggcaaccaa tggttattat
     9601 gcaaaaaaca catgagacag ggttacacaa aatctcatac aactgagatt ccgaaatctg
     9661 atggcggaac aaaagttgtg atgaatacaa aatggactca aaaagggcgt ctgttcattt
     9721 atgagtcgct gaaaaaagaa ggatatattc cagaaatcga tttattagag gaggaatgac
     9781 gatgtcacaa gaatttattt tgaaagtcag aattcaatta gcaaaatacg gaaagtctca
     9841 gaactggtta gctgatacta tcggaatttc tagaccatat atgtcggaca ttatgaatgg
     9901 gcgtagaaaa ccagataaac aaatcaagcc aattgaggca gcactagcag agttagagaa
     9961 ggagaaataa catgcaaata acaattccag ataatttagt agtttccgaa ttaactacac
    10021 agattacgaa tgctgttctt aattcattgg acgaacgatt acaccttatg aacaaatcag
    10081 tagagcttcc tccatatcca aacaaatcag aggtaaaaaa agttttaggc attggtgatg
    10141 acaaattaac acattggata agcctaggct taaaaacaca gcagtggagc aagttagaca
    10201 tcagaattga acgatcggaa ctccaaagat ttttgaaaga aaactttgag ttctaaaggc
    10261 aaaggagaat gattttatgt cctacacatt gcaacaagaa catcaaattc taggtctgat
    10321 taaacagcgt agaaaacaat tacaagatga tcgtgcagcg cttagaaaat ccgatgagct
    10381 atcagataga caagctgaac taattgcttc tgaacttgag gatttgagaa tgctagaaat
    10441 aaaaaatagg gagattagat tatgaagaag acagacacac tttttatagg attcattttg
    10501 gggttattag tgattgtagc gcaccaaagt attatcggag gaagcttgtt cgcagcattg
    10561 atggttttaa tcaatctgct tgactcaaaa gaaaggagca actatggcac gagaagaagc
    10621 gctaaaaatc ggtaaagtga ttgctgataa ttggtgggca aatagccgtt ctattatttt
    10681 aagcaagcaa catatcgaaa agaaaaaagc atggcaacaa ataaaaaagt gactccgccg
    10741 accaaagcaa tgagtcactc aaaaaatcat aactaaggag attttaacat atggaaaa
//
LOCUS       DAJSVF010000083        10754 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSVF010000083 DAJSVF010000000
VERSION     DAJSVF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.086"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            36..500
                     /locus_tag="ON550_002216"
     CDS             36..500
                     /locus_tag="ON550_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955285.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     RELGGNWYKLKIIDDTTWEYSEEMNPEPVQVSVEKQKDYQGLERYKVVDSAGVSEFIN
                     KPDYLFIVVPYEKDGVQKMILLGSSKDEKRTKEKLIHDGSELNKYKLQKTSN"
     gene            497..739
                     /locus_tag="ON550_002217"
     CDS             497..739
                     /locus_tag="ON550_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3955286.1"
                     /translation="MIAGLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            757..1059
                     /locus_tag="ON550_002218"
     CDS             757..1059
                     /locus_tag="ON550_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955287.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILGAASIITSLFGLFGQTPQGL"
     gene            1129..3183
                     /locus_tag="ON550_002219"
     CDS             1129..3183
                     /locus_tag="ON550_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955288.1"
                     /translation="MYFTIAGNTKTDKEVIKKATKEVGKRTSFLPKTIKYSVIEMEEQ
                     LHVLGKVLEQESQSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELETDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIADEQADISTHKSIFEEENEVMDQLEVDPFEETMEDEETAEVEIDPVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYKLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNGQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYETQQQELKEQEEKQRQEEKEREQLEIKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAIQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKASDSSKTKEGSSDSTVQSNSSESSESNE
                     VK"
     gene            3183..5795
                     /locus_tag="ON550_002220"
     CDS             3183..5795
                     /locus_tag="ON550_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3955289.1"
                     /translation="MRIRETTKRKDKPFNLEKKKYLSHFYSYAPAGERLPGKHDKRVK
                     QKLMYSGLVYLPLDYLVTRIPEAFSRFEIIQGSLQGVNLFQLQEMPQVFHFDSAGFWN
                     PLTPATSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPDAKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDV
                     DTATQLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPTNEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEKGDQVCLKYDHQRKEYVAWYEFSQRELKDEEGNIV
                     YRKKRGETHLPEWEKEAVLTLDESKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIV
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPAMDNMEGIMTVTAGRNMLW
                     DLFIQSYKQLRAKYGEDNADIIKENCQTHVYIMSTDDNTIEEFSKKVGNKTVEQENST
                     VNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRRDLKGNKIRPYPIFNSE
                     KSNSPYAYQYLEDFDTLGRTIHDIDIYCTHADLDLKALQIDYDYFLPDEDARDLYHGL
                     HEQLGEKIEENMFYKIVQSLENETAQKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     GKRLLLESEKVVKE"
     gene            5792..8188
                     /locus_tag="ON550_002221"
     CDS             5792..8188
                     /locus_tag="ON550_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955290.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNNVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMAGNDKSGLSDIEKDRLKALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVTPDNGEEQPDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSKKGQHGQLPKIDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKVGLPKAENLP
                     TKDSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQN
                     IVTREAAPKPIQNVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKQDIKP
                     "
     gene            8240..8572
                     /locus_tag="ON550_002222"
     CDS             8240..8572
                     /locus_tag="ON550_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955291.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFLFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYCLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            8573..9166
                     /locus_tag="ON550_002223"
     CDS             8573..9166
                     /locus_tag="ON550_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955292.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFIIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9191..9769
                     /locus_tag="ON550_002224"
     CDS             9191..9769
                     /locus_tag="ON550_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955293.1"
                     /translation="MIQDYKRIQKVLSDIDVNVTVQQAKDICEYYSKCYMATELPTKD
                     RTLYLKVVHIIKNLKPIGYVDKRPAYFNKIADQIDIHTSKIALEKILFLQNLIYYEIP
                     NYEPSYKRKVYFEYENLYVLGLSILPEERRITFYPQYNGKEMLTEEIIEKILILLERL
                     WLGLKAYVDIDYGEFQEYKIERYYPLSNEQYE"
     gene            9939..>10754
                     /locus_tag="ON550_002225"
     CDS             9939..>10754
                     /locus_tag="ON550_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3955294.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLL"
BASE COUNT         4015 a         1646 c         2173 g         2920 t
ORIGIN      
        1 atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa ctatggtttg
       61 tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat aagaaatcgg
      121 aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaaaga gaactaggtg
      181 gaaattggta taaacttaaa ataattgatg atactacttg ggaatattct gaagaaatga
      241 atccagaacc tgtacaggta agtgtagaaa aacaaaaaga ttatcaaggg ttagagagat
      301 ataaagtagt agattctgca ggtgttagcg aatttatcaa taaaccagat tatctcttta
      361 ttgttgttcc ttatgagaag gatggcgtac aaaaaatgat tttgctcgga tctagtaagg
      421 atgaaaagcg aacgaaagag aaattgattc atgatggatc agagttaaat aaatacaaat
      481 tacaaaagac tagcaattga ttgctggtct ttttatttac cctaaaggag gaattatagt
      541 ggctaaagct aaaacgccat ttgaaaaagc cgcgtctaaa atcttagaac gtcgtggtgt
      601 ttctgaaaca cagtggaaac aagaagtcct tgaaaaagca caatttgacc tattacgggg
      661 aaaagacaag gacttagaag gatatatcta tcaacgtgaa cgtgaaaagc ttgttttcga
      721 tgaaattatt aaacactaaa aggagcgaac aattttatgt ttgaatcaat tacaatgaat
      781 tttttaacac aaccgaaagt agtagctgcg gttgatattt ttagagcatt acaaactgca
      841 gcaactagtt taaaaaataa tttgatgatt tttgctggtg ttgcggccgt tgcttccatt
      901 gttattgggg ggcttttctt cctatttgga cgaagaggag ctgaaaacgg gaagatgcac
      961 gtaggttatg tattgatggg ggtaggtgtt attctagggg ctgcttctat cataacttcg
     1021 ttatttggat tatttgggca aacacctcaa ggattataag aaagtaaaaa agtaggagta
     1081 agaagtagtc gatacttttt actccttttt tatgaaagga gaatattcat gtactttacg
     1141 attgcaggca ataccaaaac agataaagaa gtaataaaaa aagcaacgaa ggaagtagga
     1201 aagcgtactt cttttttacc gaaaacaatt aaatattctg tcattgaaat ggaagaacag
     1261 ctacatgtgt tagggaaagt actggaacaa gaaagtcaat cttctgttaa gttgactctc
     1321 acgttacatg acacagaggg aaaaatagag aaagctggta cagaatacgt ttttgggccg
     1381 ctggattatc aaatttcaga aggtttctat cctaccttga tggatttaat ccgcctggat
     1441 gtgtttaaag aaacggatga aaccatgagt gtggaagagt cttatcgtaa cgagaaactt
     1501 ctacaagttt tagaagaaga gttagagacc gaccaaagaa aagtgaatca agtgagtgaa
     1561 gtcgaagcaa acgaagaagt accatgggaa cgcccaatct ttccaaagga tgaatcaaaa
     1621 gaaaaagaga aaggtattgc tgacgaacaa gctgacattt cgactcataa atcgatattt
     1681 gaggaagaga acgaggtgat ggatcaattg gaggtagatc cctttgaaga aacaatggag
     1741 gatgaagaga cggccgaagt agaaattgat ccagttgaag ctaatgaaga gccaacatgg
     1801 actgaagggg attctcagga atatattgaa ccacttatca gtgaagaagt agagaaaaac
     1861 gaaacagaga tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag
     1921 catgaatgga tgaaagaaaa aaataaatta tttttaaatg agctttattc gaaatataaa
     1981 ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa
     2041 ggcacacttt tagaacagtt agagtctgtc caatcaacaa actggcatga gcttgcacaa
     2101 gaagatttat tagctacttt tgaagaacgt caacaggaaa cagatcaaga aataaatgag
     2161 tatgcgactg aacagtcgtc gaattgggag agagcaaaag aggaactaaa taaagaagaa
     2221 caacgagtga ttgaagaaga agtacaaaaa atcaaagagc ggtacgaaaa gaaacgcgac
     2281 aatgcttatt ccttgtgtgt agaaaagatc aacaatttta ctaagacgaa tcaagaagcg
     2341 ctatcaaaag aaaaagaaaa cttgttaaaa gaacgaatta aagaacgaaa agaagagttc
     2401 taccgaggtt tacgtacaga taagttaaaa attagcaaag aattaaatgg gcaactgaat
     2461 aaactatatc aaacaacgac tgaagttctt atggaaaaac acgagcagat ccagcaagaa
     2521 ttacaagcac aaattccaaa atggaaaaaa gagtatgaaa ctcaacaaca agaattgaaa
     2581 gaacaagaag agaaacaacg ccaagaagaa aaagagcgcg aacaattaga aataaaaaaa
     2641 cgtgagcagg atcttgctga acaaaaaatg gcgatagaaa aacaacggtt agagcaagaa
     2701 aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgtcaa
     2761 gcacagttga attatctagc acaacctcaa ccagtagctg ctattcaaac gccagtgaac
     2821 aaccaagcaa atttaactcc ttcaccacag cgttccttta acggatggat ggtgggatgt
     2881 attgcaagtt taagcttgct tctaggtgga gggacagtat ttgcttttaa tcatatcaat
     2941 caatcttcac aggctgattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa
     3001 caagcaagaa aagagtatga acaacaatta gaagaattat tgaatcgtac caatcagtca
     3061 acaacagagt caagtatgga aaacagcaaa gcgagtgatt ctagtaagac caaagaagga
     3121 tcaagcgatt caactgtaca atcaaactct tccgaaagtt cagaatcaaa tgaggtgaaa
     3181 taatgcggat tcgagaaaca acgaaacgaa aagacaagcc ttttaattta gaaaaaaaga
     3241 aatatctatc acatttttat agctatgcac cagcaggtga acgattacca ggtaagcatg
     3301 ataagcgagt caaacaaaaa ctaatgtata gcggtttagt ctatctccct ttagattatt
     3361 tagtgacaag aatacctgaa gctttcagtc gatttgaaat cattcaagga agcttacaag
     3421 gagtgaatct gtttcaatta caggaaatgc cacaggtctt tcattttgat tcagctggtt
     3481 tttggaatcc tttgacccca gcaacgagtt taggtacacg cttagttgtt tctacgattt
     3541 gtgttgttcc agctgtactt atgggcttga aaatggacat gtggtttcgt ccactagcgg
     3601 atggacaaaa aggggataat cggttaatga cactaaaaga gattcaacaa atctatcctc
     3661 aaataccaga cgcgaaaggt tctttccctg gatatggtgg aattcctatc actcactata
     3721 aaaaatattg gtatatccaa acagataccg ttaatacttg tattgttggt acttctcgat
     3781 cagggaaagg acagattgaa gtattagcaa ctattgataa tttgtctcgt gcagaaaaac
     3841 aaagttcaat ggttgtcaat gatcctaaaa atgagttgta tgtagccagc aaagacgaat
     3901 tagaagccag aggctatgat gtttatgctt ataatctaac tgaaccgtta cagtcaatga
     3961 gtgataatcc tttggcttta attgtgaaat attggcaacg aggagacgtg gataccgcta
     4021 cgcaactagc aaatacattt acaaatacga tttattatga tgccaatgct tcagacaaca
     4081 aatactttaa cgaaaatgcg cagaaagctg tcaatgcgct tatttttgcg ttgttagaac
     4141 aagccgatca aacaggggat tactccaaat taacacccaa taatctcgtc gaacttcttt
     4201 ctgagattgg cggttttaat tatcaagatc ctactaatga atttaagcaa ctcaatgctt
     4261 tagatgaatt tatgaatcag ttaccacctg gaaatctagc gaaaaaacaa tatggggcga
     4321 ccaaaattgg ttctgataaa gcaaaaggca atattctatc aacagcgatc acaggattga
     4381 atccatttac cctaacaaaa attgcgaaaa tgacttctca atcaaccatt gattataaat
     4441 cggttggttt tcccaaatat ttggatttga aattacatga aagtctttta aatcaacgaa
     4501 tcgtagtgga attttatcat caaggaaaga aatttcatga agaacgagta aaagttggtt
     4561 atagaggttt ttgtgaaatc aactttgatt gtcatttaga gaaaggggat caagtttgtt
     4621 taaagtatga tcaccaaaga aaagaatatg ttgcttggta tgagttttct caaagagaat
     4681 tgaaagacga agagggtaat atcgtttatc gaaaaaaaag gggagaaacc catttgccag
     4741 aatgggaaaa agaagcggtt ctcacactag atgaaagtaa aagtaactta ttagtagagt
     4801 ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca cctgattatg
     4861 atccatctaa ccacatcgta gttagtatct ttctgagcca gttatataaa gagttatcca
     4921 cgcaatgtgt taagaccaaa ggtgataaat gtcaccgaag aattcatttc attttagatg
     4981 aatttgggaa tatgccagcg atggataaca tggaaggaat catgacggtt acggcaggtc
     5041 gtaatatgtt atgggatcta tttattcaat catataaaca actacgtgcg aagtatgggg
     5101 aagataatgc agatattatc aaagaaaact gtcaaacaca tgtatatatt atgtctacag
     5161 acgataacac cattgaagaa ttctctaaaa aagtaggaaa taaaacggtt gaacaagaaa
     5221 atagtactgt caattcttta ggcatgaata cacatatcaa tcgtaatgtt gattcagatc
     5281 gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt gtacttgcgc
     5341 cattgaaacg gcgagatcta aaagggaata agataaggcc gtatccaatt tttaattctg
     5401 aaaaatccaa tagtccttat gcttatcaat atcttgaaga tttcgatacc ttaggtcgaa
     5461 ccattcatga tattgatatt tattgtactc acgcagatct cgatttgaag gccttacaga
     5521 ttgattatga ttatttctta ccagatgaag atgctagaga tctctatcat ggactgcatg
     5581 aacaattggg agaaaaaatc gaagaaaaca tgttttataa aattgttcaa tctcttgaaa
     5641 atgagacagc ccaaaaatta ttaattcaat tttacgaaga acaagcaacg gatcgtcttc
     5701 aggaatatgt tgaaaagttg atcaaaaatg aagaaatttc tgaaatgatt gggaaacggt
     5761 tgttattgga atctgaaaag gtggtgaaag aatgatcatt ggaggaattt tttctggcat
     5821 agccgactgg tggtcaggct tattcggaga aggggatgca gatcccaaaa ccgtaacgaa
     5881 agtcttacaa gattatggta attatctgca gtatcaagat gtattcagtt ggatttgtaa
     5941 tactttttta tggggattga tcaaagggtt atataatctc aatactttat tagaaaaaac
     6001 aatttatcaa agctttgatt taaaagactt attgaacgcc gctggtgtta atgaactgtt
     6061 tcaaagtttg attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg
     6121 cttgaaattt tttatgagta aacacccacc aaaagtcaaa aatatcttgg ttaatctatt
     6181 tatggccatg atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag
     6241 taaaaacttt tacggagacg tcactgcagc aaataaagca gataaaactg gttcacctgc
     6301 ttttcaaatt attcaaaata atgtgatcga tgtaagtaca ttactagcga ctgacccgaa
     6361 taatgtagaa tccttaccaa cagataaaag aaatgcttta acagcgaaaa attttaaaac
     6421 tgctaatatt aattcagtga ttattcctaa ggaagccgct aagatggcgg ggaatgataa
     6481 aagtggatta tcagatatag aaaaagatcg actaaaggca ttacaatacc gattggaact
     6541 ggatgataat gggaaagaaa tccctgtgga gattagtgat gaaggattag ccaaatatgt
     6601 atatacttca ggatatcgtc gatacattag tagtccaggt attattttag ctggtgaatc
     6661 ttctttggcg gtggcctatt tatttatcct atttacgatc gttacttgca ttattgagct
     6721 ggtatttaaa aaattctatt tagtgattgc cgcgtcaacg gactttgaga caggacaacg
     6781 gatgaaaagt gctatcgaag atatttctca gtctttcttg ttattggcgt ttacgatttt
     6841 agaattacga atctatacat taatgctttc gggtatcggt gatttgcatg cggcaggaaa
     6901 gatcaatgga ttcttgtatg ttgtcgcttt aattgtacta accattgcgc tattcaaagg
     6961 ctctcaatca gttacgaaaa ttttcggcgt tgatacgagt ctgaaaaacg gcagcaattc
     7021 tcttcttagc atgtttgcct taggaaatat tgcgaaaaat gtgggtagtg gggctaaaaa
     7081 ccttgcaagt gctggtaaga gtggtttaag caatttaaat gatattcgta aaaatggatt
     7141 tggcaatcga aaagatgtta ctcctgataa cggggaagaa caaccagacg ataaagctac
     7201 aacagtacct aatagtaaga aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt
     7261 ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt gggttaaaag gaacagctga
     7321 agatcttaca gaaaaagcca aagacacctt agaagagaaa ttagacggca cgagagctaa
     7381 agaattggct gaagctgtga agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc
     7441 ttcagaagtc aaagaaggac taaaagatgc gtatggtgaa ggacaagtca atgcagcagt
     7501 gaaggcaaat acaacaagtg aaaaagaaaa tcaagaaggt gcagcgattc cttcagccaa
     7561 agatcaccaa gatgagttac attctaacga aaattcgatc ggagaaaaag attcaaaaaa
     7621 aggacaacat ggtcagttac caaaaatcga tccagaacaa tatcgttata aatctaacat
     7681 taattctgaa aacgaaaaac gcatttcaga tttaccaaaa cgaaatcaag aagtacaaac
     7741 ggatgatgat tccatcgatc taccaaatga taatggaatc gaagttggaa cggaaaaatc
     7801 aacgaaagtt ggtttgccta aggcagaaaa cttaccaacg aaagatagtc gtttgaatca
     7861 attcaaagag aatgatcaaa gctttgttca tcaatcacct gcaggtactg ttcaaatgaa
     7921 atcaagtcgt ttgcctgaaa atgggaattt gacaactgtt tcttacaatt tatctgaaag
     7981 tggtgagaca gagaatgcgt ccgttcaaaa tattgtgaca agagaagctg caccaaaacc
     8041 tattcaaaac gttcatacag gttacaagcc agcaaatgtg gcatttactg caccaacaaa
     8101 aataaatgtg acaagagaaa ctaaaaacag gaacaatgaa ttgaaagaac aactattgaa
     8161 ggatattaaa caggatatca aaccataaaa ggcctacgag ttaggccttt tttctatgta
     8221 tgaaaaggag aaatcattta tggataaata tattaacatg aaaaatcctc caagatttag
     8281 tggggaatgg aagtttttcg cctttttatt tttagatcta ttaacgctgg gtggttgctt
     8341 tttttgggcg aaaatagtca ataatcttgt ccacttacca ctcttttttg cactcttgaa
     8401 ttattgctta tggattgcga tagctttagt gattgtttgg cgaccctata ataacccagg
     8461 tcaacgacaa atttttgcga ttatcgaatc tattttcttt acggataaga accactatca
     8521 tgcgttagat cccaaccgaa attatcatgg tacgtataag gaggatgaat aaatgcgagt
     8581 caaaggacaa aattcagttg ataagaaatc tattttgcag tttggaaaac cagaagaacg
     8641 atcgatcttg gatttatttc cttataaatc ctgtacagaa gagggagtct tagttactaa
     8701 agaagatcat tttcaacgtt attatcgtgt agtttctaca gacgtggaag gattaaacga
     8761 acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc atgcgtacgt atgtgccaac
     8821 aattaagtta gttagtttga ccacagaaac ggatttaacg gaacaaatcg tgcaaaagcg
     8881 gcagttatta gataaaaatc gtatggaaca atccttagga agaaatctac agcgattaag
     8941 aaaatatgaa aagaaattag tagaggaaat ccaggaattg aaacgagcag aacaagaacg
     9001 tcctgactta agcttctttt tcatcattga aggaaagaca atcaaggatt tacaaaccag
     9061 aaaccgtcaa ttaatgcgat ccagtggcat tttagggtta aaaccgctct acaaaaaaga
     9121 attgatcaag atcctttatc gaatgaataa catgaatgac gaatagaata tttaaagagg
     9181 gattaaatct atgattcaag attataaacg aatacaaaaa gtattatctg acattgatgt
     9241 aaatgttaca gtacaacaag ctaaagatat ttgcgaatac tattctaaat gttatatggc
     9301 aactgaacta cctacgaaag ataggactct ttatttaaaa gtcgttcaca ttattaaaaa
     9361 tctgaaacca atcggttacg tagacaaacg tccagcttat tttaataaga ttgcagatca
     9421 aatagatatt cacacctcta aaatagcgct ggaaaaaata ctatttttgc aaaatctaat
     9481 ctactatgaa attcctaatt atgaaccatc gtacaaaaga aaggtctatt ttgaatacga
     9541 gaatctctat gtgttaggac tgagtattct accagaagag cggagaatta ctttttatcc
     9601 tcaatataac ggaaaagaaa tgttgactga agaaataatt gaaaaaatct tgattttact
     9661 agaacgtctt tggttaggac ttaaagcata tgtggatata gattatggag aattccaaga
     9721 atacaaaatt gaaagatact atcctttatc gaatgaacaa tatgaatgat gagtaggaat
     9781 ggagaagaga gaatgaaata ttcgttgata tggttagaaa aagatattcc gaaggaagag
     9841 cccactagaa aatttcctca cacgttaagc ctgggtaagt tgggaaaagg gcactctttt
     9901 aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca attaaaacag
     9961 ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta ggaaatcggg
    10021 aaatcgaaat tttaaaatcc aaagggtatg atttagattt tttaaaacaa gtgatgccac
    10081 aaggtggttt aaatttcgat gaagagtatg tgacctatgg aaatggttat tcacggtgtg
    10141 tgacagttta tcactacaca aaaaatccta ctcttttttg gttggccaat ctcatgaaca
    10201 atcctcatac agtggccaca ctcgatgtta gcacagatga aaaacaaaaa gtgattgatc
    10261 aaatcaatcg tgccttagat gaattaaatg atcgatctga taatgaacga aaagcgactg
    10321 accgaaataa aagcgcagaa gaatacctgg aactagcaga atatgctcga aaattgactt
    10381 ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga aatacgattc
    10441 aagaagtaga tgaacgagcg caggaactga ttgaaaattt aaaaggtatg gattatcgtg
    10501 cagtgaccta tctgcacaaa acgaaaagag attggcaatc gatgtttacc atgttcgatg
    10561 aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta aacattggcg
    10621 gaggtttccc gttccaccac caaacgctaa aagatccagg aggcacacaa tatggggaaa
    10681 ccatgacagg cggcccattt gtctggaatg caacgtataa aaatagtgta cggttgtcta
    10741 ctaatacctt gctt
//
LOCUS       DAJSVF010000084        10640 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSVF010000084 DAJSVF010000000
VERSION     DAJSVF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.087"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(252..1463)
                     /locus_tag="ON550_002226"
     CDS             complement(252..1463)
                     /locus_tag="ON550_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3955295.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ON550_002227"
     CDS             complement(1581..2216)
                     /locus_tag="ON550_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR3955296.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2299..2721)
                     /locus_tag="ON550_002228"
     CDS             complement(2299..2721)
                     /locus_tag="ON550_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3955297.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2739..3059)
                     /locus_tag="ON550_002229"
     CDS             complement(2739..3059)
                     /locus_tag="ON550_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955298.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3352..3543
                     /locus_tag="ON550_002230"
     CDS             3352..3543
                     /locus_tag="ON550_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955299.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3557..3808
                     /locus_tag="ON550_002231"
     CDS             3557..3808
                     /locus_tag="ON550_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955300.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            3784..3960
                     /locus_tag="ON550_002232"
     CDS             3784..3960
                     /locus_tag="ON550_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955301.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFIGLDE
                     AEYRAKKRHERQQA"
     gene            4020..4337
                     /locus_tag="ON550_002233"
     CDS             4020..4337
                     /locus_tag="ON550_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955302.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4270..4749
                     /locus_tag="ON550_002234"
     CDS             4270..4749
                     /locus_tag="ON550_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCR3955303.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPMPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            4770..5510
                     /locus_tag="ON550_002235"
     CDS             4770..5510
                     /locus_tag="ON550_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3955304.1"
                     /translation="MVKKIKAEDLSVEKGTYMIYANPGMGKTYSLGFLPGKSLVLDVD
                     GSSSTLAKHPNKENIEVWELDSSDIWQEWLDTISDLVANKSSYEKQFDNICVDNISEL
                     FKAQLEDLGKKGKNSGVPSQADYQRTDFMNLRGLRALNNLDCRIVLTAWETTDTYTEP
                     NGQFFTRSMPDIRSKILNNFLGLCDVVGRLVIKKDDDGNETRGLILKPTSNVYAKNRL
                     DERSGCLVEQLVVRAGGEPNVSTPTVSD"
     gene            5482..7005
                     /locus_tag="ON550_002236"
     CDS             5482..7005
                     /locus_tag="ON550_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3955305.1"
                     /translation="MYQLRPYQIKLVQEARKHLSQGKKGVLIQSPPGSGKSVVIAEIV
                     RLATRKGGTVLFLAHRRELLDNIRETLEQNEVDLSKVIILSAVMAKNRLNKLPKLSLI
                     ITDEGHHGKAKTYMDIYNHFKEIPRLGFTATPYRLNGEGFTDIYEEMVEGPSIQWLID
                     HHNLAPYRWYSIPLIDRSKVDFKNMSREAESSARLFESDATIQGDIVKNYKKYADGQQ
                     AIVYAPTIQVSKMIVKWFNDNGIYAVHADGKTPTKERDDIMANFKSKKITILSNVDLI
                     SEGFNVPDVGVIILCRPTQSIVLHLQQSMRGMRYRENKTSIVLDHVGNGANLGLPTDE
                     FEWSLSARKKKSNGSSSEAPRMTCSNCGQQFLLKSLLKIENKPHCPFCLQEIVSEEKE
                     NSVTFDEAVQMVELNAENAKLARLSRKKFSKKQSLELNYAIAKAKVSFEGKGNPLFKM
                     FGSLTAYQGQHYSIDQLEELSLIKDVSMESILRAYKWALEKLNSKQEEPEWVKNTFY"
     gene            7023..7529
                     /locus_tag="ON550_002237"
     CDS             7023..7529
                     /locus_tag="ON550_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCR3955306.1"
                     /translation="MTAFKVDYNEAQDFGAVPDGDYEVVIFNVTEDASKGGTEFINFD
                     MVIRNDIKQPRQNSHLFHRVWKSKETGKYNRGMIMSLAKSFGLPDGKEYQSFENFLED
                     FAMRPAKVKVKNEQSEYNGKTYDNTNIKKFETTKFPELQHQWKKNNAEKSVNSSPAFD
                     ISDNDLPF"
     gene            7529..9865
                     /locus_tag="ON550_002238"
     CDS             7529..9865
                     /locus_tag="ON550_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3955307.1"
                     /translation="MNNYERIPLELRELNQWGIYKRSWNEQRGKWSKKPYDPFTGELG
                     SSTNESKWSDFKTALSAVSTFNADGLAFYFKPPYIGIDLDDIGDDLERYLQGDVENNL
                     VYVFMNSTKTYSEISMSGKGIHIIGKAAIPGERRRKGNVEMYTEGRFFAITGNFFGNN
                     EEINEIPEIQMNFLYKRYLENETVIKQDFSKSNWSDGNDLSVNEIIQTAVNSSTGNRF
                     RLFMDGGWEKIYDSQSEADLAFANDLAFWTAGDFQKMDEIFRMSSLFRDKYDQKRGKT
                     TYGIGLLNKAISENTNHYTGKKTADDYFLSIPGITVDETKPTKFYSYDDTGNAERYLD
                     LFGSFTKYSYVNKCWYFYNGKNWEQDNIGAVRKWVDQTIEIFKNEPVSIPKDATEDEE
                     KAYIEAKEKHLRRSRNNAGKEAMTRELKHQVAILPDEFDSDDMLFNAQNGYLDLSNGI
                     LYEHDISKMFTRISNAEYTDKSDCPRWKLFLEQIFDNNTELIRYIQKAVGYSMSASIR
                     EQVMFILFGNGRNGKSVFLDIISEIMGSYAMGMQASTLMVKQGGSSGHNEDIARLNGA
                     RLVTSSEPNEGVRMDEGLIKQLTGGDKVSASYKGGHMFDYKPKYKIWLATNHKPIIRG
                     NDDGIWRRLPLIPFTVQIPLDKVDKNLKEKLMRELPGIFNWAVEGCLMWQREGLNPPA
                     DIQRATMEYRKEMDIIGAFIDECCETGPGYSIGATDLFKAYDKWARDMNEHPFSQTQF
                     GKKAADRFEKSKSKGKVVYRGIDLKKEFREFNVLVPGL"
     gene            10180..10497
                     /locus_tag="ON550_002239"
     CDS             10180..10497
                     /locus_tag="ON550_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCR3955308.1"
                     /translation="MTAEIEIQNAIRRELPKYGHFVYRGNVGKVKTIDGRWFDTGLPK
                     GWPDLFGWTKEGKFFAIEVKNEKGKLRPDQVKFGDFLQKQPVLYGVARSVEEALKIVE
                     ESS"
     gene            10494..>10640
                     /locus_tag="ON550_002240"
     CDS             10494..>10640
                     /locus_tag="ON550_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3955309.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIH"
BASE COUNT         3636 a         1686 c         2054 g         3264 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 tcgttattta atggtatttt aaatgaagat aagcgaattt acagcatttt taaatgctta
      181 tataccgcaa attatcccaa gtttttatat atcctacccg agtttatacc cgaaaatata
      241 attttttagt gttattttat atcttttttt aatagttttt caaacgctgt agttgctgta
      301 tctttttctt tgcgtaagac atggccataa acattcattg tagtattaaa atctcggtgt
      361 cctaatcgtt tttgaagctc ttttggagtc atattaggat cgctaagcaa ataggtagct
      421 gatgtatgtc taatgtcgtg aaaacgaatg tgacgcaaat tgtgacgttt ggtgaatcgt
      481 ttgaacttct tatacagata acttccacgt ggtaaagttc cgtcttgttt tgtatcaaat
      541 ataaaatagt gtcttaattt ctttatattt aatttccaac gaattttttt attctctttg
      601 atcaattcat ctaacatatc aaatagataa gcaggaccgg ttactatttt ttcatcatca
      661 tcatttttca aacctggcag caaccgaata tctgattttc catccacttc tgaaatccgc
      721 tgatgaaatc ttatttcttg tttgtcaaag tcaatatctt tttcttctaa agctgcaatt
      781 tctccttgtc ttgctcctgt aataaatgct agaacaatta aagttttaat ttctatgctt
      841 tcctcaaatg ctgcttttag cattgcatga atttcttctt ccgaataagg ttcttcgata
      901 ttctttttta cgcctttttc ttttggtatt tttacatttt ccacaggatt ttctttaatt
      961 gctccgtact catttttagc catcaaaaat aagttgttaa cggcgtataa catacgaagc
     1021 tttgtatttc ttgataaagg tttatcagag tttcttccag gatcttttgt attgattctt
     1081 tgagcattaa caacaatttt ctttatcatg tatggtttta tatcgattag agggataaaa
     1141 tcaaatttat caagaaatct attctcgatc attcgcttat agttatgata agactttggc
     1201 tctaaatttt tttcggcctc tgaaagccat atttttctat aaaattcacc aaaagttata
     1261 ttgaataaat ccaacgaatc atcggtcatt ccttcgaatt gctctaacca gatgttcaat
     1321 tctttatatg ccgctctttt actattcgct tttatagttt tgctttttct ttttgccgag
     1381 ccatcaggtt tgtaccctaa tacagctctt agtctatatg tttcattgtc tacttgttct
     1441 aagtatcctg ttacttctga cataattgaa tcagctctct ttctctgata caataggtac
     1501 tataaagaag cctattgtgt aggtttgttt tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttatga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tgtgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atatccctca cttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggcttttttt actgtgcata agagtatttt ttcttgaaat atgactggca
     2341 aacattaaaa cattctgttc ttaacttatt attgatagag tagaattcca tgaaattttc
     2401 taatttgaac tgagattcat cagtcaattc gttctcaata aagatattta gtaaaatcat
     2461 aattgcgatt ctatcagctt cagcttcgaa ttttgaatga aaagttgtag agttatcgta
     2521 cagtactgaa tattcaaaat gtgaagcaat gaaatgaccg agctcatggg ctaaatgaaa
     2581 agcttcagaa ctgtcttcgt gtagtttttc attcaaaaat actattcttg gttttggata
     2641 ataaaaacct ggttcttcca tttccatata gattaacttt aaattatact cactcagcat
     2701 ttctttcaac tttaaataca tacaacccat cactccaact attcattttc ctctaaagct
     2761 ttagcaattg caatcgcttt acgcattgtc tccttagata tttcttttcc atcaaaagaa
     2821 aatacagtat catcttctga taaatccaca tgtttaggcg tttctttttc ttctctacct
     2881 agaagatagt ctacagagac attgaaatag tcagctacct tttgcaaatc tcttgctttg
     2941 ggttctgttg ttttccatcg atagaaaaga ttttcgctaa atcctaattc taaagcgact
     3001 tctttcatgc ttttatctct ttggctagct aatgatttta tcctttcaaa cagtgtcata
     3061 ataatgttct ccaatcctaa acaaataaaa gtacacaaaa tgtaaattat actgttgaca
     3121 taagtataag tttgtattat tatttgtttg taagttaatt tgatagaaaa aaagcaaact
     3181 aaaaatacac cttatagcat taagtttggc gaccgattgc ataataaagg gtttgttgta
     3241 ggcttattta actatgttta tatagtatac gattgtatac tattagtcaa tagtttatac
     3301 gttttttcta tcattttgac ttacaaatct atatgtaagg ggagtgaata gatgtcaaat
     3361 ctagataacg gccgtgctgc aatcaagaag tttatgcgag aaaacggagt aactatggaa
     3421 gatttggcta ctgcatatgg atacacacgt gttcgaatgc agcaaattat tgatggccat
     3481 tggagtggac caaaagctaa taaaactatc ttagaaatca tccgtgatta cagaattcga
     3541 taggaggaat taatcaatgg aacaactagc tttagttaac ctctcagccc tcaaagcttc
     3601 tttggcagaa tctgagattg caaatgaagt gtgggataca aagcaagctg cggaatattt
     3661 gaaaaccacg acacgcacgc taactaaaga tgccgaatct ggaaagattc cagcagcgaa
     3721 agttggcaga gagtggaggt ttagtagtat cgctttgtat cagtatctaa aaggaggaaa
     3781 aacatgggca aattcaacag agcactagta ttcagcgcac cgctaatcat ctacgcttta
     3841 gggctttggg gaaatagaca agcgttgata ggaacgattg tgtacatggt ttggattttt
     3901 atagggcttg atgaagctga gtacagagcg aaaaaaagac acgaacgcca gcaagcatga
     3961 tcgtgtcaag gaaataaact atctttctat attttaccac agaaaggaat gaaccgtaaa
     4021 tgctaattgc aacggataca ctagaccgca tctttttaaa agacgaatac aaactgcgca
     4081 atatagatgc gtcaggaatt ttagtatttg atctttatga caatggaaaa attggtatct
     4141 atcaagcaag tgatattgaa gaaacaaacc ttgctttcga gcaaattgat gattctgtgg
     4201 aattggattt agatgaggca atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg
     4261 cacaaaagaa tggcaactct ttaccaactc agcgagtcat atatcaaagt cctggaactg
     4321 gcagaacaat tggatgaaga aattcttcgt gatactctcg attcgattaa tgaagcgatc
     4381 gagtataaag cagaaaactt agcaaaaata gttaaagaag tagaagggaa agcagagtta
     4441 atcgatagtg aaattaaacg tctacaggaa cgtaagacat cgcttttgaa caatgctaag
     4501 agtatcaaac actatttaca agaggaaatg gaaaagactg gtaaaacgaa gattaaaggc
     4561 gaattattca acattggaat tcaaaataat ccagtatcgg tcaatgtaat caatgagaac
     4621 ttaattccaa aaggattctt tacccctatg cctcccaaat tggataaaaa gcaattgaag
     4681 gaggaactga agcatggaga tattcctggt gctgaactcg tccaaacaaa aggtttgagg
     4741 ataagatgat gcaagtggag gtatgagtaa tggtaaaaaa aataaaagcc gaagatctat
     4801 cagttgaaaa aggaacttac atgatttacg caaatcctgg tatgggaaaa acttattctt
     4861 taggatttct tccaggtaaa tcattggtat tagatgttga cggatcatca tcgacattgg
     4921 caaagcatcc taacaaagaa aatattgaag tgtgggaatt agattcttct gatatttggc
     4981 aagaatggct tgatactatt tcagacttag tcgcaaataa atcttcctac gaaaagcaat
     5041 tcgacaatat ttgtgtagat aacatttctg aattgttcaa agcgcaattg gaagatttag
     5101 gcaagaaagg aaaaaatagt ggtgttcctt ctcaagccga ctatcaaaga acagatttta
     5161 tgaatttgcg aggtcttaga gcattgaata atttagattg ccgaatagtc ctgactgcat
     5221 gggaaacaac agacacatat acagaaccta atggacaatt ttttacaaga tcaatgccag
     5281 atataagatc aaaaatttta aataattttt taggcttatg tgatgtagtt ggcagacttg
     5341 taataaaaaa agatgacgat ggtaatgaaa caagaggctt aatactaaag cctactagta
     5401 acgtttatgc aaaaaacaga ttagatgaac gtagtgggtg tttggtggaa caattggttg
     5461 ttagagcagg cggtgaaccg aatgtatcaa ctccgaccgt atcagattaa gctagttcaa
     5521 gaagccagaa aacatctatc tcaagggaaa aagggagtat tgatccaatc gccaccagga
     5581 agtggcaaat cagttgtaat tgcagaaatt gttcgattag caacaagaaa aggtggcaca
     5641 gtactatttc ttgcccacag gagagagtta cttgataaca ttcgagaaac tcttgaacaa
     5701 aatgaagtag atttatcaaa agtcataata ttgtcagctg taatggctaa aaacaggtta
     5761 aataaattgc caaagctgtc tctgataatt acggatgaag ggcatcatgg taaagcgaaa
     5821 acttatatgg atatttataa ccattttaaa gaaattccta gactaggttt tactgctacg
     5881 ccatacagac tcaacggaga aggatttaca gatatatacg aagaaatggt agaaggacca
     5941 tccatacagt ggttgattga tcatcacaat ctagcgcctt atcgttggta ctctattcct
     6001 ttgattgatc gttctaaagt agattttaaa aatatgtcac gtgaagctga aagttctgcg
     6061 cgattattcg agtcagatgc cacaattcaa ggtgacattg ttaaaaatta taaaaaatat
     6121 gcggatggtc aacaagcaat cgtttatgct ccaacgattc aggtaagcaa gatgattgtt
     6181 aaatggttta acgacaatgg aatatatgcg gttcatgcag atgggaaaac acctaccaaa
     6241 gaacgtgatg atattatggc aaatttcaaa tcgaaaaaaa tcactatatt atcaaatgtc
     6301 gatttaatca gcgaaggatt taatgtacca gatgttggag tgattatcct ttgcaggcct
     6361 acgcaatcaa tcgtcttaca tttacaacag tctatgcgag gcatgagata tcgagaaaat
     6421 aagacttcga ttgtgttaga tcatgttggg aacggagcta acttaggttt acctactgat
     6481 gaatttgaat ggtcgttaag cgctaggaaa aagaagagta atggaagcag cagcgaagcg
     6541 cctagaatga cttgctcaaa ttgtggacag cagtttcttc tgaagagctt attaaagata
     6601 gaaaataaac cacattgccc gttctgctta caagaaatag taagtgaaga aaaagaaaat
     6661 tccgttactt ttgatgaagc ggttcaaatg gtggaattga atgcagaaaa tgccaagcta
     6721 gcgcgacttt caagaaagaa attttcaaaa aaacaatctt tagaattaaa ttatgcgatt
     6781 gcaaaagcaa aggtaagttt tgaaggtaaa gggaatccgc tttttaaaat gtttggctca
     6841 ctcactgctt atcaaggaca acattactcc atcgatcaat tagaagaatt atcgttgatc
     6901 aaagatgtat caatggaatc aattttaaga gcttataaat gggctttgga aaaactcaat
     6961 tcaaaacaag aagaacccga atgggtaaaa aatacatttt attaaggaag aggtaattaa
     7021 ttatgacagc atttaaagta gattacaatg aagcacaaga ttttggagca gtaccagacg
     7081 gagattatga ggtagttatt ttcaacgtta cggaagatgc ctcaaaaggc ggcacagagt
     7141 ttattaattt tgatatggtc atcagaaatg atattaaaca accacgacaa aacagtcatc
     7201 ttttccatag agtttggaaa agtaaagaaa ctggcaaata taatcgtggg atgatcatgt
     7261 cgctagcaaa atcatttggc cttcctgatg gaaaggaata tcaatcattc gaaaatttcc
     7321 ttgaagattt tgctatgaga ccagcgaaag taaaagtaaa gaacgaacaa tcggagtaca
     7381 acgggaaaac gtatgacaat acaaatatta aaaaattcga aaccactaag tttccagaac
     7441 tccaacatca atggaagaaa aacaacgctg aaaaatctgt aaattcctca ccagcatttg
     7501 atatttcaga taatgatctt ccattctaat gaataattac gagcgtattc ccttagagtt
     7561 gcgtgagtta aatcagtggg ggatttataa acgctcatgg aacgaacaac gagggaaatg
     7621 gagtaagaaa ccttatgatc cgtttactgg agaattaggg agcagtacaa atgagagcaa
     7681 gtggtctgat ttcaagaccg ctctctcagc tgtttcaact tttaatgcag atggcctagc
     7741 tttttatttt aaaccacctt atataggtat tgatttagat gatataggtg atgatttaga
     7801 aaggtatctt caaggcgatg tagaaaataa cttagtttac gtttttatga attctacaaa
     7861 aacatattca gaaatatcaa tgagtggcaa gggaatccat attataggta aagcagcaat
     7921 ccctggagaa agacgtagaa aagggaatgt cgaaatgtat accgaaggta ggttctttgc
     7981 tattacaggt aacttctttg gtaataatga agaaattaat gagatacctg aaatccaaat
     8041 gaatttctta tacaagcgat atttagaaaa tgaaacagta ataaaacaag atttttctaa
     8101 aagtaattgg tcagatggaa atgacttatc tgtcaacgag atcatacaaa cagcggtgaa
     8161 ttcttccaca ggtaatcgtt ttagattatt catggatgga ggatgggaaa aaatatatga
     8221 tagccaatct gaagcagatt tagcatttgc aaatgattta gctttttgga ctgctggaga
     8281 ttttcaaaag atggatgaaa tattccgaat gtcttcgttg tttcgagata aatatgatca
     8341 gaaacgcgga aagacaactt atggaatagg gcttttaaat aaagccatat ctgagaatac
     8401 caatcattat actggcaaaa aaacagctga tgattatttt ctttccatcc caggtatcac
     8461 tgtggacgaa actaaaccga ctaagtttta tagctatgat gatacaggaa atgcagaaag
     8521 atatcttgat ttgtttggat catttacaaa atacagttac gtaaataaat gctggtattt
     8581 ttataacggt aaaaactggg aacaagacaa tatcggtgcc gttcgaaaat gggtagatca
     8641 gactatcgag atattcaaaa acgaacctgt ttcgatacct aaagatgcga ctgaagatga
     8701 agaaaaagcc tatattgaag caaaagagaa acatttaaga aggtctagaa acaatgctgg
     8761 aaaagaagct atgacacgtg agttgaaaca ccaagtagcg atacttcctg atgaatttga
     8821 tagtgatgat atgctgttca acgctcaaaa cggttattta gatctttcta atggcatttt
     8881 atacgaacac gatatttcta aaatgttcac tcgaatttct aacgctgagt atacggataa
     8941 aagtgattgt ccacgttgga agctgttttt ggaacagata tttgacaata acactgaatt
     9001 gattcgctat attcaaaaag ctgttgggta ttctatgagc gcatcgatca gagaacaagt
     9061 catgttcata cttttcggta atggaagaaa tggtaaatct gttttcttag atatcatttc
     9121 tgaaataatg ggaagttatg ccatgggaat gcaggcatca acattgatgg ttaaacaagg
     9181 tggtagcagc ggccataacg aagatatcgc acgattaaac ggcgcacgtc tagtaacttc
     9241 ctcggagcca aatgaaggcg taagaatgga tgaaggttta atcaaacaac taactggcgg
     9301 agataaagtg tcagcatctt ataaaggagg ccacatgttt gactataaac ctaaatataa
     9361 gatttggctt gcaactaatc ataagccaat catcagagga aatgatgatg gtatttggcg
     9421 aagattgcca ttaattcctt tcactgtaca aattccattg gataaagtgg ataagaattt
     9481 gaaagaaaaa ttgatgcgcg aattgccagg aatattcaat tgggcagtag aaggatgttt
     9541 gatgtggcaa agagaagggt tgaatccacc ggccgatatt cagagagcta caatggaata
     9601 tcgaaaagaa atggatatta taggtgcttt tatagatgaa tgttgcgaaa caggacctgg
     9661 ttattcaatc ggggcaacgg atctatttaa agcatacgat aaatgggcaa gagatatgaa
     9721 tgaacatcca ttcagtcaga cccagtttgg taagaaagct gcggacaggt ttgagaaatc
     9781 aaaatcaaaa ggaaaagttg tttatcgagg gattgattta aaaaaagagt ttagagaatt
     9841 taacgtatta gttcctggat tgtgaaacaa aaaagtttca caaatgtgtg taggtagaca
     9901 gttgggtaga tagtaagtag atagtttttt acaaactgtc tacccgataa aacctttggg
     9961 gctgtaaggc tcattactgt taggtagata gtagggatag tttatatata tagtaaataa
    10021 aataataaaa ataaggaaat atataaaaat acagaagcaa cctagaaata actgtctact
    10081 gtctacccga actggttaat acttagagcc acaagggata gagcgattat aaactgtcta
    10141 cccatgtata tatactatct acccgagcgg gaggcgctta tgacagcaga aattgaaata
    10201 caaaatgcca ttcgaagaga attaccaaaa tatggtcatt ttgtttatag aggcaatgtg
    10261 ggcaaagtga agacgattga tggcagatgg tttgatactg gattgcccaa aggatggcca
    10321 gatttatttg gatggactaa agagggaaaa tttttcgcca ttgaagtgaa gaacgaaaaa
    10381 ggaaagttga gaccagacca agtgaagttt ggtgattttt tgcaaaagca accagtccta
    10441 tatggtgttg cacgatcagt ggaagaagca ttgaaaattg tggaggaatc atcatgacaa
    10501 cagaagaagt gattcaaatg cgaattcgaa gcattcaacg tgaaattgac gatctagaac
    10561 gaacaaaggc agtgatggtc aatgaaacgg cgaggaaggc aatcgatttg cacatagaga
    10621 atttaagaag ggaaatccat
//
LOCUS       DAJSVF010000085        10636 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSVF010000085 DAJSVF010000000
VERSION     DAJSVF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.088"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            23..646
                     /locus_tag="ON550_002241"
     CDS             23..646
                     /locus_tag="ON550_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955310.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="ON550_002242"
     CDS             1096..2145
                     /locus_tag="ON550_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3955311.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ON550_002243"
     CDS             2142..3023
                     /locus_tag="ON550_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3955312.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ON550_002244"
     CDS             3026..3796
                     /locus_tag="ON550_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3955313.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ON550_002245"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ON550_002245"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR3955314.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ON550_002246"
     CDS             5107..6270
                     /locus_tag="ON550_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3955315.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ON550_002247"
     CDS             6387..6797
                     /locus_tag="ON550_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3955316.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ON550_002248"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ON550_002248"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3955317.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ON550_002249"
     CDS             9370..9654
                     /locus_tag="ON550_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955318.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ON550_002250"
     CDS             9814..10050
                     /locus_tag="ON550_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3955319.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ON550_002251"
     CDS             10047..10445
                     /locus_tag="ON550_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR3955320.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3713 a         1773 c         2252 g         2898 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggct
//
LOCUS       DAJSVF010000086        10574 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSVF010000086 DAJSVF010000000
VERSION     DAJSVF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.089"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            12..84
                     /locus_tag="ON550_002252"
     tRNA            12..84
                     /locus_tag="ON550_002252"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ON550_002253"
     tRNA            88..160
                     /locus_tag="ON550_002253"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ON550_002254"
     tRNA            168..249
                     /locus_tag="ON550_002254"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ON550_002255"
     tRNA            267..339
                     /locus_tag="ON550_002255"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ON550_002256"
     tRNA            351..422
                     /locus_tag="ON550_002256"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ON550_002257"
     tRNA            439..524
                     /locus_tag="ON550_002257"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ON550_002258"
     tRNA            535..608
                     /locus_tag="ON550_002258"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ON550_002259"
     tRNA            618..691
                     /locus_tag="ON550_002259"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ON550_002260"
     tRNA            711..783
                     /locus_tag="ON550_002260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ON550_002261"
     tRNA            811..884
                     /locus_tag="ON550_002261"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ON550_002262"
     tRNA            898..971
                     /locus_tag="ON550_002262"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ON550_002263"
     tRNA            995..1084
                     /locus_tag="ON550_002263"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ON550_002264"
     tRNA            1097..1170
                     /locus_tag="ON550_002264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ON550_002265"
     tRNA            1189..1264
                     /locus_tag="ON550_002265"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ON550_002266"
     tRNA            1266..1336
                     /locus_tag="ON550_002266"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ON550_002267"
     tRNA            1350..1423
                     /locus_tag="ON550_002267"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ON550_002268"
     tRNA            1448..1535
                     /locus_tag="ON550_002268"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ON550_002269"
     tRNA            1557..1628
                     /locus_tag="ON550_002269"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ON550_002270"
     tRNA            1651..1740
                     /locus_tag="ON550_002270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ON550_002271"
     tRNA            1753..1826
                     /locus_tag="ON550_002271"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ON550_002272"
     tRNA            1845..1917
                     /locus_tag="ON550_002272"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ON550_002273"
     tRNA            1921..1993
                     /locus_tag="ON550_002273"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ON550_002274"
     tRNA            1998..2068
                     /locus_tag="ON550_002274"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ON550_002275"
     tRNA            2081..2154
                     /locus_tag="ON550_002275"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ON550_002276"
     tRNA            2166..2237
                     /locus_tag="ON550_002276"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="ON550_002277"
     CDS             2503..3696
                     /locus_tag="ON550_002277"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR3955321.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ON550_002278"
     CDS             3826..5298
                     /locus_tag="ON550_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3955322.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ON550_002279"
     CDS             5381..6169
                     /locus_tag="ON550_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR3955323.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ON550_002280"
     CDS             complement(6236..6904)
                     /locus_tag="ON550_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR3955324.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ON550_002281"
     CDS             7019..8731
                     /locus_tag="ON550_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955325.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ON550_002282"
     CDS             8734..10506
                     /locus_tag="ON550_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955326.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DAJSVF010000087        10512 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSVF010000087 DAJSVF010000000
VERSION     DAJSVF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.090"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            33..257
                     /locus_tag="ON550_002283"
     CDS             33..257
                     /locus_tag="ON550_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955327.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            272..709
                     /locus_tag="ON550_002284"
     CDS             272..709
                     /locus_tag="ON550_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955328.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            702..1409
                     /locus_tag="ON550_002285"
     CDS             702..1409
                     /locus_tag="ON550_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955329.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            1790..1969
                     /locus_tag="ON550_002286"
     CDS             1790..1969
                     /locus_tag="ON550_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955330.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2062..2247
                     /locus_tag="ON550_002287"
     CDS             2062..2247
                     /locus_tag="ON550_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR3955331.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2281..2712
                     /locus_tag="ON550_002288"
     CDS             2281..2712
                     /locus_tag="ON550_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3955332.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            3649..4923
                     /locus_tag="ON550_002289"
     CDS             3649..4923
                     /locus_tag="ON550_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3955333.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            4977..6404
                     /locus_tag="ON550_002290"
     CDS             4977..6404
                     /locus_tag="ON550_002290"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR3955334.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            6417..7289
                     /locus_tag="ON550_002291"
     CDS             6417..7289
                     /locus_tag="ON550_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3955335.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            7384..8379
                     /locus_tag="ON550_002292"
     CDS             7384..8379
                     /locus_tag="ON550_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3955336.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(8984..9091)
                     /locus_tag="ON550_002293"
                     /pseudo
     CDS             complement(8984..9091)
                     /locus_tag="ON550_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9207..>10512
                     /locus_tag="ON550_002294"
     CDS             9207..>10512
                     /locus_tag="ON550_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3955337.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVGEKLKISFEPTDSF
                     QYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAERTGINVFKNELNESRLKTYDTL
                     LNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDNSSYPKPISDYINYLNNMKNRYE
                     QKNVNNIASYIRDEIIMGKYEIDEKDNSVSFRQKFGKNRYRKERIPFHVVSSSIKSLY
                     GLDYYLENSSKEGDYLIIDEPELSLHPENQVKLAKVITDIVESKIKLITSTHSDLFVR
                     ELTNIVIENKLNSKNNKKNKLSDKNIGIYNFVGNNDINELEKI"
BASE COUNT         3779 a         1463 c         1977 g         3293 t
ORIGIN      
        1 attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgaatg
       61 taaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
      121 tagaaacccc tttagtagag gacgcaccga ctatgctatt tgaagaccta gtgaagattg
      181 caggatatga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
      241 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag aattactatg acgaatttgt
      301 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
      361 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
      421 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacattgg aagaactgaa
      481 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaaacc
      541 aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatggaa aatttatcga
      601 aacgaaaaaa gccaagactg tttcaagtga atggttggac tactttgaaa atgtcaacaa
      661 gtatggcgca tgttacatca tgaaaaagga ggacaacgaa aatgagtaat gtggtttatc
      721 aactaacagt ggaagatgtt tctcactttt taacaaacaa agaacatttg aatgaagtag
      781 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
      841 tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac gaaggtcaca gtcagccata
      901 atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac
      961 gactagaaac agaaaaatac atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa
     1021 actttgtcag ctacacgatt tgtaggaaac actattactc agaatcagaa tattattcaa
     1081 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga
     1141 cggtagacga ttattacctt gtatttacta gatttctgac gtgtgggacg gaatctatcg
     1201 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
     1261 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct
     1321 acacttacga cgatacagaa gaaatccaaa atgagcttga ctgtaaacga atgccatatc
     1381 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
     1441 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
     1501 ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac gaccgataac ctaagagaaa
     1561 gaaggaaatc catatgaata aaatcattcc tctgttgttc gtactgtttg ttggcgagat
     1621 acttatgcgt catgctgcag ctgtacattt aaacttcgta gtattcttag gaatactggt
     1681 attttgtttg atcttttggc gatttgtatt agcccttgtg agtctactat tcaagatgat
     1741 tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
     1801 aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagga
     1861 actcaaacag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
     1921 gattgcacaa gaaaaagcag ggtttgatgt accagcactc aaaaagtgac gtcacttttc
     1981 atttgaagaa acaaaagtga tagcacttta cgcacgtgtc gatacgtgtt atgattattt
     2041 tgtaaggagg tatagtgcac aatgccactt acgggaaaag aaatgcttaa gttattgaaa
     2101 aaaaatggct gggttgagcg aaggcaagag ggatctcatc atcatcttta taaggatggt
     2161 gttagaatca ctgttcctgt tcatgccaat caagatcttg gccgaggact tgagcaaaaa
     2221 attttaaagg atgcgggatt gaaataatct cgcattcgat taaagaaagg attgactgaa
     2281 atgttaagtg agcctttagc aaaaacctat cctgctattt ttagcccaga agaaggtggt
     2341 gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca caggaattaa cgaagacgat
     2401 atatcttatg gaattgctat ggcacaagag gtgttaggta tggttttagc agattatatt
     2461 gagcatgaag atctacttcc agaaccaaca ccaatcaata agatttctgt tgaagatgat
     2521 tcttttacta ctttaattcg tgtagatgtt gccaaatatt tgaaagacac agaacttgtc
     2581 aaaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc tggcataaat
     2641 ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct tcgttctgga
     2701 agaaataatt agaactcaaa agagtcagaa acttaaaaat ctttggcttc tttttttact
     2761 acaaagatgg cttctttaaa ttatttgcct gattgattta aaaaaattgc gcaaagacta
     2821 cgagctaatt aagttgctcg tggtctttct ttatgaaaac tttttagatc tagataaaaa
     2881 cattgagaca ctttgaaaaa tttttttaat agtaatgggc gcttctatgt caagctgatt
     2941 cttctttcgg taaattaatt ttacttagaa tatcactatc ttgataaaaa atggtgtatt
     3001 cacaaattct ggcataaaaa atacctactt tctagttaag gttcctgaat aagtcgcaat
     3061 ccaattattt ccagctgatt tataactttt taaatataat tttccataat accaataacc
     3121 attacttttt tctgtccaat ttcgttattg agaagatcta gaaggccagc caactttatc
     3181 aaaaatcatc attcctgtca ctcgtatcgt tttagaagct gctcgagtaa gtaatggaga
     3241 aactgttaaa taattttaag taacagttaa gtcgttagtt gggttatcaa tggctattcc
     3301 gtccttatct gaacctcgaa acccaatcat agaaaaagtt ttttgaacaa gttttaacag
     3361 ataaatcaag ttatatacta gaaaattcgt aaaatatatt tttaatgtca taatcgtttg
     3421 atttttctgg attgacattt atttataaaa aatagtgcca ttaaaaaaga aaagtgacac
     3481 accaataaaa gtcatatcct gtgacattat atggcattat ccaaatagaa taaccggttg
     3541 acatgtaagc ggattccttt tataatttta ggagtataag tccttttcta aaaatattaa
     3601 ccggttatca tgaaagggga tacaaatata tcgttggagg ataaaaatat gtcaaaggtt
     3661 aacgtattta aaaatcaatc ctatttacaa agttcagcta cattattact attttttgct
     3721 tcttggggtg tttggtggtc attttttcaa ctttggctaa catctgaatc aaatggttta
     3781 gggttatctg gcagtgctgt aggaacagta ttctcggcaa attcgttagt taccttaatt
     3841 ttgatgttta tttatggaac attacaagat aaattgtata ttaaacgaaa tttattaatt
     3901 tttgcttctg tattagcgac acttgttgga ccatttttta tatggatata cgggccattg
     3961 ctagataaca attttaattt aggcattatt atgggagcac tatttttgtc agctggatat
     4021 ttagcttctg taggagtttt tgaagctgtg tcagaaaggt ttagtcgttt atttggcttt
     4081 gaatatggac aagcaagggc gtggggatca tttggttatg ccttggtagc gcttttggca
     4141 ggatttttat ttgtaaaaaa tcctcattta aacttttggg cgggatcttt ctttggttct
     4201 ttactattgt taaatttatt attttggaac cctaaagttg aacgggaagc aaatcaaaat
     4261 tttaatcaag aacaagctga atcaaatagt attccttctt taaaagaaat gtttgatcta
     4321 atgaaactgc ctcaattatg gacgataatc atctttattg tttttacatg gacattttat
     4381 acggtattcg atcaacaaat gtttccggga ttttatactg gtttgttttc aacatcagct
     4441 aatggtgaaa aaatatatgg gacattgaat gctattcaag tattttgtga agcgttaatg
     4501 atgggaattg ttccaatcat tatgagaaaa ttaggggttc gaaatacttt gttattaggt
     4561 gtaaccatta tgtgtgtacg aattggattg tgcgggtttg cctcgacacc attatctgtt
     4621 tcatgcataa aaatgttgca tgctttagaa gtaccattat ttacattacc aatgtttcgc
     4681 tattttacac ttcattttga tacaaagcta tcagcaaccc tctatatgat aggatttcag
     4741 atagctgctc aaattgggca agtgatttta tcaacaccat tgggaatatt aagagacaac
     4801 gttggctatc aaccaacatt taaaattatt tctcttattg tattactagc aggcatatat
     4861 gcattcttta ttcttaaaca agatgataga gatgttcaag gggatccatt tattcgagga
     4921 taatggaaac caacagaata aaaaacaatt ttatctagaa agtgagcgaa tatattatga
     4981 caaaaagagc tatagaatta acaaatacac gttatcgttt gggatatcat gtgtctgcac
     5041 catcaggatg gattaatgat ccaaatggat tttgctattt tgatgggtat tatcatgtgt
     5101 tttatcaaca tcatccatat agtgctgaat ggggaccaat gcactgggca catgctagaa
     5161 gtaaggattt agttcattgg gaatctttac cacttgcttt gactcctgga gatcaagaag
     5221 atgaaggtgg ttgtttttca ggaagcgcta ttgaaaaaaa tggtgtgttg tatctgttct
     5281 atacaggaca tcactattat ggagacaatg atccagatca tttttggcaa aatcaaaaca
     5341 tggcgtatag tacggatggc attcacttta ctaaatatga aaaaaatcct gtaatagcaa
     5401 aagcaccaga agataacaca catcatttca gagatccaaa agtatggaaa cacgatgaaa
     5461 aatattacat ggttttgggt agtcaagaaa aagatggatt aggtcgtgct attgtttatc
     5521 agtcgttaga tttattaaat tgggattaca ttggaccaat ttctaaagcg aaggaagtac
     5581 ttacagaagg atttatgtgg gaatgcccag acttttttga attaaatgga aaatctattc
     5641 ttttattatc ccctcaggga attgaagcaa atggaaaaga ttatttaaat ctatatgaaa
     5701 caggctactt tatcggtgat tatgattatg agacagcaaa attcattaga ggaaattttt
     5761 atgaattaga taagggacac gatttctatg caacacaaac aactttatct ccagatggta
     5821 gacgtattgt tatggcttgg atggatatgt gggaaagccc tatgcctgaa aaagtagacg
     5881 gatgggcagg agcattaacg cttcctcgag aattagagtt aattggagat catttgtata
     5941 tgaaacctgt gagagaatta gaacaacttc gagttggcag cggcgttgaa acaaaagtta
     6001 atttggcagg agaaataata atcgctcaag atgcttcatt caaagaagct ctaattgatg
     6061 tactattgac taaatcaaaa aatcaagaaa tcaaattttc attaaaaaca aaagatgaag
     6121 agttaattaa tattttttat tctaaggata aaaatgaatg taccttaact cgtaacggca
     6181 aaaatgatcc aaggtatgga accattcaga tgtgtgatag attatcactt cgagtgttta
     6241 tcgataaaag ttcggcagag atttttatta atgatggcga gcttgttttt acagagcgtt
     6301 attatacaga aaatcaacca agtatgcatt tgagtgttag tgaagaagct tttgtctcga
     6361 gtacaattta tcatctaaaa aaagatgcaa tttcttacga gtaaggaggt tttatgatgt
     6421 taattggtag catagaagca ggtgggacta aatttgtatg tgccgtggga gacgaggatt
     6481 accgagtaca agaccaaata atttgtccaa cgaccacccc cagggaaaca ttgaaaaaaa
     6541 ctgttgacta ttttaaacag tttgatataa atgcattagg tattgcatca tttggtccaa
     6601 tagaaattcg aaaaaattca ccaaaatcag gatatattac gtctacacca aaggtgggat
     6661 ggcgaaatgt taatgtgtta ggttatttac aagatgagct gaatgttcca atgtcttgga
     6721 caacggatgt gaacggatca gcttatggag agtacatcat gtccacttta tcgaatgaaa
     6781 aaataaattc attagtttat tacaccgttg gtactggcgt tggtggtgga gctgttgttg
     6841 acggaaaact attaggatcg tttggtcatt cagaaatggg ccatacgttt gtaaaacgcc
     6901 atcctgatga tattgatttt aaaggaattt gtccttatca tggtgattgt ttagaaggct
     6961 tagttgcggg accaacgttt gaagcacgtt ttggaaaaaa gggtgaagac gtttctttaa
     7021 cggatcctat ttgggatgta atggcctatt atctagcaca agctgtgatt caaacaacat
     7081 tgattatacg accggataaa atagtatttg gtggaagcgt agtaaatgaa aaattgttag
     7141 aaaaaattcg tgttaatgtt aatgaattat tggatgatta cgtggaaata ccaccgttaa
     7201 gtaaatatat tacgcgtcct ttaattaaaa ataatggatc ggcaacacta ggaaactttg
     7261 ctttgggctt gaatcaatta caagaataaa acatagaaaa aatatcgatt atcaggtata
     7321 gtagttctat agagttcttt ttttcgatag ttaattattg cattatttag gagagacaag
     7381 agaatgcgcc caaaattaga agatgttgcc aaaagagcaa atgtatcaaa aacaactgtt
     7441 tcacgtgtat taaacaatcg tggctattta agtcaaaaaa cgattgataa tgtttataag
     7501 gctatagaag aactcaatta tcaaccgaac gttgtagcgc gtcaattatt tcaaaagaaa
     7561 actaacattg tcgggttact atttcctaca gtagccaatc ctttttttag tgaactcgtt
     7621 gaggcattgg aaaaaaagtt atatgaaatt ggttataagg tattaatagg caattcgatg
     7681 aataataaag aaaaggaaac aaattattta aatcagttat tgtctgatca agtagatggt
     7741 ttaattgttg gtacacataa ccaagggatt caggaatata aatatcagaa tttaccgatt
     7801 gttgcaattg atcgagtgat gaatgaagat attccagttg ttgaatccga taactacaat
     7861 ggtggaaaac ttgcaaccaa gttattaatt gcacaaggtg caaaaaatat tattcataca
     7921 aacggtccaa ttgacttaca gactccggct aatcgtcgga gactagccta tgaagatact
     7981 atgaaagcat atcagttaat tccacgaact gtgacattag atttcaatat ttcttatgta
     8041 aagaaaaaac agattttttt ccaaatgttt gaggattatc ctaaaataga tggtattttt
     8101 gcttcaaatg acatcgatgc ggccctaata ttacaagtag caaaagaaaa ggggttaaat
     8161 gtaccagctg atttattagt agtcggatat gatggtactt taatgactcg gagtatatta
     8221 ccagatctga caacagtaat acaaccaatt aatgatatag cagacacagc tgttgctatt
     8281 ttgatgaaac gaataaataa agaagaaaca aaaaaggaat atattttacc tgtcacatta
     8341 tggatgggga agacatcagt aaagaaaaag gacaattaaa tttataaaga ctatcaatga
     8401 taatttcatt attaattcgt gatataaaaa gttgttaaat ctcttataat agactatgat
     8461 acaaaatcat gcttttaatc tgtgtagatg ttgccaaata tttgaaagac atagaacttg
     8521 tcagaaaaac cttaacaatt cctcaatggg cggataagct aggcaaaagg gctggcataa
     8581 acttttcagt ccttctaaca gaatcaattg cagataaagc agataacatt cttctttctg
     8641 gaagaaataa ttagaactca aaagggtcag aaacttaaaa gtttttgatc ctttattttc
     8701 aagtctgatt tgacagattt tcggcagctc atttatttat ggtcagaaat agttgctgaa
     8761 attttaatct attagcaaat agtccagaac gtatgaagag gattcggata tcatttgtca
     8821 ttaacaccat aaatatttat aaaattcgtg ttcacctaca attaaattta atatataata
     8881 ttcgttgtaa gggcttataa ttttttgtta atgaacaaga tatgtaattg atagtgtcaa
     8941 tagctattca aaaatgtagg cttttggccc ataaataaca aggttactta tatgataact
     9001 ttttttcttt ataatcttta atccgataga gctttccagt agtttgaata atcttagcgt
     9061 ggtgcactaa tcgattgatt agtgcatttg tgttcagtcc attgagacat aatagtggat
     9121 agttttactg agaaaattga taaaaaatag agatataatt caaatacgta tataatgaaa
     9181 ctaaagataa atacggagga aaatatatga ttataaaaaa cttaggaaat gttaaatcaa
     9241 tagatataca cgttaaagat ttgctgattt ttactgggaa aaataatacg ggaaaaacat
     9301 ataccagcta tattttatat ggaattttat cagaactctt tggagcaaga tttttcaatt
     9361 atttttctca tacagaaatt gaaccacatt tacaaaatag tttaaatatt ttattagtta
     9421 gcaaagactc tataaggaaa aaatttaata atgatgcaaa aaaattttta gaagaaaatt
     9481 taaaaaaaat tgcagttcgt aactttaaag taagtgaaaa taacttcaaa aatctttctt
     9541 ttactatttc agattcagaa attagtgagc tcactggtga tatctttaat tatgagatca
     9601 aaagatacag tataagtggc attgttataa caattcaaaa tgtaggagag aaattaaaaa
     9661 ttagctttga gccaactgat tcatttcagt atcttagcaa agatattgaa attgaagata
     9721 tttataaaat tttagctcaa gagctatcta aaaatgttat tagtttgcca aaggtttttt
     9781 attttccagc cgaaagaact ggtataaatg tctttaagaa cgaactaaat gaaagtcgct
     9841 taaaaacata tgataccttg ctgaatactg tgcaatttac taatttaact aaccaaaaaa
     9901 agaaagctgc cctcaagtac gatctgttaa aaaagaatat tgagctgtta atggagacaa
     9961 cggataatag ttcttatcca aagccaatat cggattatat taattatctg aataacatga
    10021 aaaatagata tgaacagaaa aatgtcaata atatagcatc atatattaga gatgagatta
    10081 ttatgggtaa atatgaaatt gacgaaaaag ataattctgt aagttttaga caaaagtttg
    10141 gtaaaaatag atatagaaaa gaacgaattc cttttcacgt tgtgtcttct tcgattaaat
    10201 ctctatatgg attagattat tacttagaaa attcaagtaa agaaggagac tatcttatta
    10261 ttgatgagcc agaattgagt ttacatccag aaaatcaggt taagttagca aaagtaatta
    10321 cagatatagt tgagagtaaa attaagctaa ttacttctac ccatagtgat ttatttgtac
    10381 gtgagcttac aaatattgtt attgaaaata aattaaatag taagaataat aaaaaaaata
    10441 aattgtctga taaaaatata ggcatatata attttgtagg aaataatgat ataaatgaat
    10501 tagaaaaaat tt
//
LOCUS       DAJSVF010000088        10478 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSVF010000088 DAJSVF010000000
VERSION     DAJSVF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.091"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(136..804)
                     /locus_tag="ON550_002295"
     CDS             complement(136..804)
                     /locus_tag="ON550_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3955338.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            938..1225
                     /locus_tag="ON550_002296"
     CDS             938..1225
                     /locus_tag="ON550_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955339.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1487..2446
                     /locus_tag="ON550_002297"
     CDS             1487..2446
                     /locus_tag="ON550_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3955340.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            2715..4253
                     /locus_tag="ON550_002298"
     CDS             2715..4253
                     /locus_tag="ON550_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR3955341.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            4250..5215
                     /locus_tag="ON550_002299"
     CDS             4250..5215
                     /locus_tag="ON550_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955342.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            5202..6524
                     /locus_tag="ON550_002300"
     CDS             5202..6524
                     /locus_tag="ON550_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3955343.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      6587..6691
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6765..7508
                     /locus_tag="ON550_002301"
     CDS             6765..7508
                     /locus_tag="ON550_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3955344.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            7523..8515
                     /locus_tag="ON550_002302"
     CDS             7523..8515
                     /locus_tag="ON550_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3955345.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            8527..9264
                     /locus_tag="ON550_002303"
     CDS             8527..9264
                     /locus_tag="ON550_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955346.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            9254..9975
                     /locus_tag="ON550_002304"
                     /pseudo
     CDS             9254..9975
                     /locus_tag="ON550_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
     gene            complement(10059..>10478)
                     /locus_tag="ON550_002305"
     CDS             complement(10059..>10478)
                     /locus_tag="ON550_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR3955347.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3458 a         1798 c         2175 g         3047 t
ORIGIN      
        1 aaaaaaacaa actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag
       61 attttactga ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt
      121 agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc
      181 tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt
      241 attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc
      301 caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat
      361 cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc
      421 gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt
      481 gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa
      541 aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa
      601 tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa
      661 aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat
      721 attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc
      781 tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact
      841 tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg
      901 gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac
      961 aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca
     1021 aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt
     1081 tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg
     1141 tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga
     1201 gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa
     1261 aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga
     1321 acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta
     1381 ttaacgccga ttgtgccgat tgtaaaatta agctagacaa ctaaaaaagt catttgtgct
     1441 acactcaaaa tagttccgtc caaagaatta taaggagtga gtacaaatga cctataccca
     1501 tcttacaacg gatgaacttg taatgataga gtcttatttc aaaataaatc aatctgttgc
     1561 gaaaactgcg catttcttga atcgttcaag acaaacgatc cataaagtat acctgttctt
     1621 caagcaagga aaatcagcct tagagtacta tcaacagtat aagaaaaaca aatcaaactg
     1681 tggtagacgc ccgcttgttt tacctgagga acaatcagaa tatattcaaa gaaaggttgt
     1741 tcaaggatgg acacccgatg tgattgttgg tcgtgcagcg tttcctattc gttgttctgc
     1801 tcgtaccatt tatcgtatgt tcaaaaaggg gctatttaat ccttctgact taccgatgaa
     1861 aggcaagcgt aaaccaaatg gacatcaaga aagacgtgga aaacaagctt tccgtcgctc
     1921 tattcatgaa cgtgaaaaag attatagcca attctcaaat gagtttggtc accttgaagg
     1981 tgacactatc gtaggtttga aacataaaag tgctgtaatt accttagttg agcgattatc
     2041 aaaagttatc atcacattga aaccgtgtgg tagacaagcg attgatattg aaaacaaatt
     2101 aaatcattgg tttgaatctg taccgaaaaa tctattcaaa tccatcactt ttgattgtgg
     2161 aaaggaattt tcaaattgga aacagatcag taatgccaat gatattgcca tttatttcgc
     2221 tgatccagga acgccgtctc aaagaggcct aaacgagaat tctaacggat tgttacgtag
     2281 agatggttta ttgaaatcta tggatttcaa ttcagtagat gaatctttta ttcaatctgt
     2341 cgcatcgaaa cgaaataata ttcctagaaa atcactgaat tatcgaacac ctttggaagt
     2401 atttttgagt tacgtaagta ttgatgattt gtctaactta atttgacaat taagataaaa
     2461 taaaagttgt gtgtgtaaaa aagatatagc tgattccaag tgacaatttc gtatagattt
     2521 ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg atccgacttt gttgcttgga
     2581 ttcttttttt gtcagcatat ttagaggtat ttttttcttt tttttgttcg tgctgtaagg
     2641 ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg tttatactta aagaataaca
     2701 gaaaggaagg tcgcatgaat aattttatac aaacattaag tgaaagaaaa ggcgagttgc
     2761 tggaagctgc atttcaacat ctgtccattt ctctcatttc tttgctgatt gcagcactga
     2821 ttgctattcc tttagcgatt tgggcagcca atcataaaaa aatggcagaa gtcttattgc
     2881 agatcacggg tgtgctccaa acgattcctt cacttgcctt attaggttta cttatcccat
     2941 ttgtagggat cggaactgtt ccagcattga ttgcacttgt tatttacgct ttgcttccga
     3001 ttttccaaaa cacgtatatt ggtctagctg aaattgaccc gtctattgaa gaagcagcgg
     3061 tagcatttgg tatgtccaga atgagacgct tgctgaaagt agagcttccc atcgcattac
     3121 ctgtcattat ctcaggtatc cgaactgcct tagttttaat catcggtacg gctactttgg
     3181 cagcattgat cggagcaggt ggattaggga catttatttt gttaggtatt gaccgaaata
     3241 cacctgtttt gacattgatt ggtgcgatca gttctgcctt gcttgcaatt atattcagcg
     3301 gactgattcg ctggttgcag cataagaaac ctcgatattc catcattact ttagcagcag
     3361 tggtgtgttt atttggcggc gtttctttat atcagtctgc cttgtttaaa aatgaaacca
     3421 tcacgattgc tggaaaatta ggagcggaac ctgaaatcct gatccaaatg tataaagaat
     3481 tgattgaaga caacacaaaa acagaagttg aattaaaacc gaattttggc aagacgagtt
     3541 ttttgttcaa tgcactggat caaggtcaaa tcgatatcta tcctgagttc acaggtactg
     3601 tcctagaaag cttggtgaag gtacctgaag caatgaaaag tgctgatttg acaaaagaaa
     3661 aaacatatga cgaagcagaa gaattattga ataagcaatt cgatatgaca ttattaaaac
     3721 caatggctta ccaaaatacg tatgccttgg cagtcaagag atcttttgcg aacgaacatc
     3781 aacttaaaaa gatttccgat ttggcagcta tctctgatca gatcaaagcg ggattcacac
     3841 tggaatttat tgatcgttca gatggatata aaggaatcca agaagcatat ggcttgaatt
     3901 tttcaagtgt acaaagcatg gagccaagtt tgcgttacca agcaatcaat aatggagatg
     3961 tcaatattgt ggatgcctat tccacagata gccagctcaa ggagtacgat ctagtgactc
     4021 tagaagatga tcgagggtta ttcccagctt atcaaggcgc accgctgatg aaaaaagatt
     4081 ttgctaaaga acatcctgaa atcgttaaag cactgaataa attaagcgga aaaataacag
     4141 aggatcaaat gattgatatg aattaccaag tcaatgtcga aaagaaaaag ccgacagaag
     4201 tagcacgaat cttcctgaaa aatgaaggat tagtaaagga ggaagatcag tgactaaatt
     4261 aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa
     4321 tctggcaatc aaaaaaggag aaatctttgt tttggttggt ccttccggta gtggaaaaac
     4381 aactactttg aaaatgatca atggattatc taaaccatca gcaggagaca tctacttcaa
     4441 aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg gatatgtgct
     4501 gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa aatattgaag tcatccctga
     4561 aatgttaggt tgggaaaagc agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt
     4621 tggcttatct cctgatattt atcgagatcg tatgcctaga gagttgtctg gaggagaaca
     4681 gcaacggatc gggatcatcc gagctattgc tgcaagccca gatgtgattt tgatggacga
     4741 gccatttagc gcattagatc cgatttcaag aaactcatta caagaattgg ttttgtcctt
     4801 acacgaagaa ttgggcacga ccatcgtttt cgttacgcac aatatggaag aagcaatcaa
     4861 gctaggtgat cgtattgcat ttatgaagga tggagaaatt atccaatgtg acacaccaga
     4921 gcagttattg atgaatccta aaaacgacta cgttcggcat ttctttgatg aaccgaaaca
     4981 gacgaaagag tggcgagtag aagatttagt cgtcaatgga tatttcttaa atgaaatacc
     5041 agaaaatgct cgtcaacaag tttgctttga tacgcctatg aaagaagtat attcgcttct
     5101 ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga agtattgggt cgctgacaag
     5161 taaagaaatc tttgcttttt taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa
     5221 tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa gaagcaggta
     5281 aaacagtact agtggtcgaa gaagatctat ggggcgggac ttgtcctaac cgtggatgtg
     5341 atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg
     5401 gaaaaggttt taacgagatc ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa
     5461 catttacgga tccagtccca gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc
     5521 atctttcagg aaccgctcgt tttttggatg attcatctat tgaagtcaac gaagaagtct
     5581 tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc tacgatctta ccagtggagg
     5641 gaaaggaata cttgaaaact agtgcagact ttttatcatt gcctgtactg cctaaagaaa
     5701 tcatttttat cggtggtggt tatattgctt ttgagctagc tacgattgcc aatgctgcag
     5761 ggagcaaagt aaccattgtc catcataatc aacggccatt aaaagaattt gaggcgtctt
     5821 tggtagagga agccgtccat caaatggaag catctggcat tcagtttgct tttggtgttg
     5881 aaacccaaaa gatcatttca gaaggtactc gctatcgttt agtcggaaaa gagacggaat
     5941 tagtagcaga tatgattttt tgtgcgactg ggagacagcc gaatacagaa tctttagcac
     6001 ttgagcaagc gaatatcgtc tttgataaac acggaattgc agtcaatgat tatctgcaaa
     6061 cgagcaatcc aaaaattttt gcgtgtggag atattgtttc aaggaaaacc ccaaaattga
     6121 caccagtcgc cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg
     6181 aacctatcaa atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag
     6241 taggagttac aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca
     6301 gttggttcac ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc
     6361 agcaaaatta tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg
     6421 atttgacact cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg
     6481 ggtatccgac attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa
     6541 agcgcttgtt gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg
     6601 gatgtcgtag acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa
     6661 tactggtgaa gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt
     6721 caagcgtttt ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt
     6781 atccagcagt cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga
     6841 aaatacctgc ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg
     6901 cacaagtatt actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa
     6961 tagctagcgt tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa
     7021 tgtcttttta tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat
     7081 tacttaccct atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa
     7141 gctattttcc gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa
     7201 tcatcttttt gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac
     7261 cagcgggttt atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg
     7321 gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg
     7381 taaaaaacag ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga
     7441 gtttattgct gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata
     7501 ttttttaaga gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct
     7561 tttatccgct tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa
     7621 aaaagtgaca cttgttttgg attatgtacc gaatactaat catactggca tctacctagc
     7681 aaaagaaaaa ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga
     7741 taattcgaca agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca
     7801 agaagatgtc acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac
     7861 agtgatcaaa cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc
     7921 aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt
     7981 aaaagctgtg atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa
     8041 tggagaaggg ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg
     8101 ggatatgatc aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt
     8161 agatgaacgg ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag
     8221 tgatccagaa ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat
     8281 taaggatcca aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac
     8341 gtttttagaa gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg
     8401 gggattgatg gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt
     8461 gatcaagcaa gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg
     8521 atagatatgt caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaaatatta
     8581 gaagacatcc atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga
     8641 gccggtaagt caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg
     8701 atcaaagtag acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa
     8761 gaagatatgt tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc
     8821 aatcatctaa caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac
     8881 ggctatgaac attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg
     8941 ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta
     9001 gatgctatga ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag
     9061 cggactactt tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt
     9121 cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga
     9181 cggtcacgtg aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg
     9241 gcaggtgaat tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc
     9301 actccttcag caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg
     9361 gaaggaaaac agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc
     9421 ttggaaagca gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat
     9481 catcggtgaa atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa
     9541 gtatttgagc gacagcttgc ttttgcagcc ataaatgaga aacctgttgt actgcacaca
     9601 aaaggatgcg aaaaagagat cttaaattat atccaagctt accctaatca ctatttgatc
     9661 cattggtatt cttctttgga ctatcaacaa gactatattg atttaggctg ttatttcagc
     9721 attggtcttg atttgaaaaa gaatccagca gtctggcaac tagctaaagc agtcccaatc
     9781 gaccacttac ttattgaaac ggatggacta catgcagcag agtgggcttt aggggggatt
     9841 ttttcagaaa aagattatgt ctcactgctt gaagaacagt taaagacaat cgctgagatc
     9901 aaagagctat cagttgaaga agttaaaaaa aatacttatc aaaacttgga acgtttcatt
     9961 agattaggta aataaaaaag agatgtgaca caactagtgt cgcacctctt tttggctttc
    10021 tttgtataaa agtaaactgt ttgattaata aatgaatctt agtaccaacc gtttgcttcc
    10081 cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg ttctgctact
    10141 ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa ttggtaacgt
    10201 ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat
    10261 tcttttgcag agcttgtgct tgctggagta gcagcagttt ctgttacagc aggagcaggt
    10321 tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg tacaacaggt
    10381 tgttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca
    10441 gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DAJSVF010000089        10474 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSVF010000089 DAJSVF010000000
VERSION     DAJSVF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.092"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            111..890
                     /locus_tag="ON550_002306"
     CDS             111..890
                     /locus_tag="ON550_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955348.1"
                     /translation="MEKPFVKSLTAVAVGAGAIVICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVIQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNISY"
     gene            936..1880
                     /locus_tag="ON550_002307"
     CDS             936..1880
                     /locus_tag="ON550_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3955349.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGSDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1896..2675
                     /locus_tag="ON550_002308"
     CDS             1896..2675
                     /locus_tag="ON550_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR3955350.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKRTAPARPKATNDKTAPSRRSRSRV"
     gene            2687..3385
                     /locus_tag="ON550_002309"
     CDS             2687..3385
                     /locus_tag="ON550_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR3955351.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3413..4177
                     /locus_tag="ON550_002310"
     CDS             3413..4177
                     /locus_tag="ON550_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR3955352.1"
                     /translation="MIDLHCHILPGIDDGSENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4191..6008
                     /locus_tag="ON550_002311"
     CDS             4191..6008
                     /locus_tag="ON550_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3955353.1"
                     /translation="MFILTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSIFILLFINRRYS
                     LRLVIFTYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6019..6651
                     /locus_tag="ON550_002312"
     CDS             6019..6651
                     /locus_tag="ON550_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3955354.1"
                     /translation="MKAIIIGAGGFGKEIAFLLQSVSRYELIGFVDDSLKMKNQELLG
                     KPVLGTIDSLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDAQIGNYNSIFPSVNISGHVILGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            6689..7771
                     /locus_tag="ON550_002313"
     CDS             6689..7771
                     /locus_tag="ON550_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCR3955355.1"
                     /translation="MSEEGYEQEYVKEAFDTNWVAPLGANVNGFEKELAKYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPVFVDSDYETWNMK
                     PEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKNELSKLSNIQFMPENNWNEPNYW
                     LSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGDDVAETLFDLGVC
                     LPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            7761..8381
                     /locus_tag="ON550_002314"
     CDS             7761..8381
                     /locus_tag="ON550_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3955356.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNTPEKFKGS"
     gene            8508..9677
                     /locus_tag="ON550_002315"
     CDS             8508..9677
                     /locus_tag="ON550_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3955357.1"
                     /translation="MCRVLNILFQYENIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNISKLIELGCQIIETPIDRRGINPKTDFKLFFNY
                     CNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFL
                     YRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVH
                     FLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYH
                     GYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGY
                     LCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            9723..>10474
                     /locus_tag="ON550_002316"
     CDS             9723..>10474
                     /locus_tag="ON550_002316"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3955358.1"
                     /translation="MSNVSNPLVSVIMGAYNSEKTIGRAIDSIIKQTYLHWEMIICDD
                     GSTDGTVKIIRKYVEKDKRIKLVQNKKNSGLAYSLNHCLKYSSGKYVARMDADDISLP
                     ERFAYQVGFFEKNPEYDVVGCNRIVFDENGDSGIRKSKEIPTINDMYKGSPFAHPTIM
                     MKRSVYTILGGYRVNKDTTRSEDLEMWFRFFNEGFRGYNLQRVLFKYQETSEDFHKRS
                     LRAAYGITKVLLRGYKLIGVSKYKYIYAFKPI"
BASE COUNT         3809 a         1625 c         2112 g         2928 t
ORIGIN      
        1 tttctgtgaa tatattagtt taattatgtt ttttaaaata aaaagctgtg atttttatgt
       61 aagtgaatta tatcttaatt tatctatcat caatacttag gagtgaatct atggaaaagc
      121 cattcgtcaa gtcattgact gctgtggcgg taggtgcagg tgctattgtg atctgtttgt
      181 ttggttacca tatcaataat cagcggcaac atcagcaacg gatcaattat gcagaatctg
      241 ccatcaccaa tcaaaaagac accttgactt ctttatcgaa agaagtagac aagctgtata
      301 gtacaaaaga aaaaattttt ctaaaccctg aaataacaga agaaacaatt acaagtttgt
      361 ccaataagct atcctctgtc aaactttcag cagatgatta cgacatcaaa gaatcagaat
      421 tgcctaaaga agctgttgca atccaagatg agaagaaagc aatcgtgaca cagttagata
      481 gtgcagaatc aaaactgaag atccagacag ctgtcaacga tttatttact aaaaatgtat
      541 caaactggca gcaagctgta gacgatgtca tcatcaaaga aaaggtgaca agtgcagatg
      601 tcgctcgtgt acgtgaaaat atgagctttt ttaaagatag tccttggaaa acggttgtta
      661 tacaatatct aggattcgct gatacccaga tcgcgcaagt cacgcaacta gatcagttat
      721 tcgatacgat gttaaaggat ggtcaagtga ctgccaccgc tacttacgat caatatttga
      781 cggcgctcag tcaaatagag cagatccgga atgaaaaaat aagcgtagca tacgcaacaa
      841 aagcacaaac tgttgctcag caaatgggct atagcaatat aagctattga cagttaaagc
      901 gttgattcaa gcaaggttag caaacggagg aaactatggc aagacgaaga agaaaatcaa
      961 gagggaaaaa aatatttctg attattttag cagttcttat cgtattagta ttagcaattg
     1021 taggagtagg agcaaaatta tatctggatc tctcacattc tattgagaaa acttacgaat
     1081 cagtggaacg aacccaagga gaacgaacga aaccagttga tctggacaaa caagaatctt
     1141 tctctgttct attgatgggg attgatactg gagatttagg tcgggtcgat caaggaagat
     1201 cagatacgat gatggttgcc acagtcagtc ctgaagacaa acagacaacg atcgtcagca
     1261 ttcctcgaga tacgtatgtc gacatcgtag gtagtgacac aaaagacaag atcaaccacg
     1321 cttacgcatt cggcggagct gccatggcga tggataccgt acaaaaatat gtagacatcc
     1381 caatcgatca ctatgtctct atcaatatga aagggctaaa agaacttgtt gatgcagttg
     1441 gtggtatcga agtcaataat gacctcactt tctcacaaga tggctatgac tttacaatcg
     1501 gtaaaattac tttgaacggg gatcaggcat tatcttattc tcgtatgcgt tatgaagatc
     1561 caaacggtga ctatggtaga caagagcgtc aaagaaagat cgtagaaggt gtagcgaaaa
     1621 aagtattgag tctaaacggt gtcagcaact atcaatccgt tttgaatgct ctagagtcga
     1681 atatgaaaac agacatgagc tttgatgaaa tgaaaaaaat tgctttagat tatcgggagg
     1741 catttggtac gatcaagcaa gatcagctgc aaggagaagg cttcatgcaa gataatatct
     1801 cctatcaaaa agtcagtgat caagaacttg atcgtgtgaa aaaagaatta aaagatcaaa
     1861 tgaatctaga gaataaatag gaaagtggag aaaaaatgga agagacaatc agcttgaaag
     1921 aaatttttga aacattacgc aaacacttgg taacaatcat catctcaatg tttgctggct
     1981 tggccatctc aggaatcgta acatttttca tcttgacacc aaaatacagt tcacaagcac
     2041 aattgatcgt gacactgcca caatcagaca cagcaaatgt caatgatgtc aatacgaacc
     2101 tgcaaatgat caatacgtat aaagatatga tcgtcagtga tttagtacta aatgaagtca
     2161 aagatcgttt agaaacagaa gacaatgtga aaatgactgc tggacaaatc aaagatgcga
     2221 tctccgtcaa tcagtcacaa aattcacaaa tgttctcgat ccaggcaata agcacgaatg
     2281 cagtgactgc tcaacagatc gctaatacaa cttcacaagt tttccaagaa aacgcaaaag
     2341 acgtgatgaa tgtcgataaa atctcgatta tttcaggtgc agttgccaat acgacacctg
     2401 tttcgccaaa taataaattg aatctagtga ttggcttagt attaggaatg atggtaggcg
     2461 taggtttggc attcttattc gaattacttg atcggacagt gaaagatgaa aaatatgtga
     2521 cggatacatt aggattccca atcttaggaa cagttccaga aatgtcagcg aaagaattga
     2581 acgctggtat ccgtaaaaaa agaacagctc ctgcacgacc aaaagctaca aacgataaaa
     2641 cagccccatc acgcagaagc cgctcaagag tctaggagga agaaaaatgg cacgaacaca
     2701 gaaacaaaaa gcaacacaaa ctcatgcagt cagtttgatc acattaacaa acccatcttc
     2761 accaatcgcc gaacaatatc gtacgatccg gacgaatatc cagtttgcct ccgcagctgg
     2821 tcagcaaatc aaaacgattg tcgtgacttc atcaggtcct ggagaaggga aatccaccac
     2881 tgcagcaaac attgcagtcg tttttgctaa atccggtcaa cgagttctac tagtcgatgc
     2941 agatttacgt aaacccgtcg tctataagac attccagttg aacaatgcca gtggattaag
     3001 tacagcactt agttcatcag gaagtgtagc agatgtgatc caacgaacgc cagtcgagaa
     3061 cttgtcgatt ttgcctagtg gacctaagcc acccaaccca tcagaattgt taagctcgcc
     3121 aagaatggat cagatcctag ctgaagcaag acagttattt gacgtggtga tattcgatat
     3181 gccaccagta gtagccgtaa cagatgcaca gatcatgtct tcaaaaacag atggcacttt
     3241 attggtcgta cgtgaaaata tgtcacgtaa agaatccttg actaaagcaa aagaactgct
     3301 agagatggta caagcacgag tcttaggtgt cgtatacaac ggagcagaac acagcaaaga
     3361 tgctggttat tactacggaa attaaggaat aagaagagag gagacaagag aaatgatcga
     3421 tctacattgc catatcctac caggaatcga tgacgggtca gaaaacttag aagcaagtat
     3481 tgcaatggct gaaaaagcga ttcagcaagg cattacgcat attttatgca caccacacca
     3541 caataatgga aaatacagca atgaaaaatc acaagtcatt tctctagtcg cttcgttaca
     3601 agccgaatta gagaaaagac agttgccttt gacgttattg gaaggacaag aagtacgtat
     3661 taccggaacg ctcatcgaag acatccaccg agatgaaatt ttatttacag atttagatga
     3721 tacatatctt ctgatcgaat ttccgacact ggaagtgcct ttatatgcag aacggctttt
     3781 ttttgagttg ttagaactag gaaagacacc agtgatcgtc catccagaac gcaacgcgca
     3841 ttttcgagaa gacccaaatc atttgcttcc gttcctagat atgggatgtt tggcccaact
     3901 tactgcgcca agttatgttg gtgtatttgg caaagacatc cagaaaacag ccaaaacaat
     3961 ggtcaaacat aatctcgtgc agatggctgc ttctgatgca cacggggtct ctaagcgcac
     4021 cttttactta aaagagtgct atgagcagat tgccaaggag tttggtaagg aaaaagtagt
     4081 acagatgaaa caagtcgcaa aggatctgat caacggggat gagatcaatt atccaatcta
     4141 tgaagaggta aaaaagaaga agtttggttt attttagggg gagttaaaaa gtgtttatat
     4201 taacaagaag aagaaaaatt gccacattgg ttgttgtgga cagcttgtta ttaatcgctg
     4261 caaatattgc agcagctaaa ttcatgaagc cgtttgtatc tataccaatg gatcttattt
     4321 taatctctat cggtttgtcg attggttttt atttatttta tggtagttta ttcaaagttt
     4381 tcactcggat caatcgctat acaaatttaa gagagatcat tgcaattttt agttcactat
     4441 ctgcaagtgc tgcctcaagt atcttcatac tgctgtttat taatcggcga tacagtttac
     4501 ggttagttat ctttacttat ctgctttcgc tcttattgat catcggtagc cgattgatct
     4561 ggcgtatcta catcgaaaca aaaaatatga gatatgtatc agcagattct gctaaaaata
     4621 cattgattgt tggtgctggc gaaggcgggc gtattttata caacagcttt cttggatcca
     4681 aaacggctca agatatccac gttgtcgggt ttgtagatga tgatccaaat aaaagaaata
     4741 cgtatctttc aggaaaaaaa gtattaggtg cattaaaaga tattccggaa ttgattgaaa
     4801 aatatgacat tcaaatggta acgatcgcta tcccttcctt atcaagaaaa aaattacgac
     4861 gaatcttcga gttagtcgaa tccgctcgtg tgaaagtcaa cacgatgcct tctatagaag
     4921 aacttgcttc agggaaaatt agcgtatcaa aattaaaaac aattgacgta gtggatttac
     4981 tgggaagaga tgaggtaaag ctggatattg aatccatcaa agatcagatc acagacaaag
     5041 tcattcttgt caccggcgca ggaggatcga tcggttcaga aatctgtcgg caaatcattc
     5101 aattcaatcc tgcaaagcta ttgcttttag gacatgggga gaactcgatc tacttgattg
     5161 accgtgagtt acgcacgcat catcaaaatt gtcctacaga aatcgtaccg atcatcgcag
     5221 atatccaaga tcgcgagaaa atcaatgaga tcatggaaca atatcatcca gatatcgtct
     5281 accatgcagc tgctcataaa catgtaccac tgatggaata taatccaaaa gaagcagtca
     5341 aaaacaatat tttcggtaca aaaaatgtag cagaagcagc taaagcagca aaagtgaaaa
     5401 acttcgtcat ggtatcgaca gataaagcta acaatcctcc aaatgtcatg ggttcaacaa
     5461 aacgaatcgc tgaaatgatt gtgactggat tgaatgaaga aggctgtacg aaattctcag
     5521 ctgtcagatt tgggaatgtc cttggttctc gcggtagtgt gattccagta ttcagagaac
     5581 aaattgctca aggtggtcca atcactgtaa cagactttcg tatgactcga tactttatga
     5641 cgattcctga agctagtcgt ttagttattc aatctggcgc attagcaaaa ggtggcgaaa
     5701 tttttattct agatatgagt gaaccagtga agattgtaga cctagcgaaa aacatgatcc
     5761 gattgagcgg gtatagcgaa gacgaaatcg aaatcattga aacaggtatc cgtccagggg
     5821 aaaaactata tgaagaacta ttgttggata aggaaagaaa tgatgaagca gtttatgaga
     5881 aaatctttgt aggaaacatc aaaggctact caatacaaaa agtgatggat tttgttaaga
     5941 gcctgccgca agacgatgaa caattggcta aagacattgt cacttttgca aatgcttcga
     6001 ataagtaggg ggagtaaagt gaaagcaatt attataggag ctggtggatt tggcaaagag
     6061 attgcttttc tattacaaag tgtctctaga tatgaattga ttggttttgt cgatgattcg
     6121 ttaaaaatga aaaatcaaga attattggga aaacctgtat taggaacaat agattcatta
     6181 atagaattag aagaagagac tgctattttt ctgggaattg cttcgccaga tatcaaggag
     6241 aaaatatatc aaaagataaa taaaaataat aaactgattt tccctaattt agttgcacca
     6301 agtgcactag tgggcataaa tgttcagtta gggattggca atattttgat gccctatacg
     6361 acttatactg ctgacgtagt tttaggaaat ttcaatatga tcaatatcgg ctctactatc
     6421 ggacatgatg ctcaaatcgg taattataat tcaatttttc caagtgtaaa tatttcagga
     6481 catgttatat taggagataa aaatgaaatt ggagtaggta ctaaaataat acaaaatcta
     6541 agaattggaa atatgaatat tataggtgca ggaagtgtag taataaaaaa tattaataat
     6601 aatactaaaa atgttggcgt gcctaccaaa gtaatagaaa gatgggatta agttggaaaa
     6661 taaacgaata ttattagcat ctcctcatat gagtgaggaa ggatatgaac aagagtatgt
     6721 aaaagaagca tttgatacaa attgggttgc tcctttaggt gcgaacgtga atggttttga
     6781 gaaagaatta gcaaaatacg tgggagctag tcatgctgcc gcattgtctt ctggaacagc
     6841 agctatacat ttagctttga aagcggctgg ggtcggtaaa gatgacatag tgatttgtca
     6901 gtccttgacg ttctcagcta ctgctaatcc aattctctat caaggagcta tacctgtatt
     6961 tgttgatagt gattacgaaa cttggaatat gaaacctgaa catttgcgaa cagcactaga
     7021 aaaatatccc aacacaaagg ctgtattagt agtacatttg tacggtttat cagctaattt
     7081 agacgaaatt actaaaatat gcaaagaata tgatgttact ctaattgaag atgctgcaga
     7141 atcattagga acaacgtata aaggaaaata tactggtaca tttggcgact atggaattta
     7201 ttcttttaat ggaaataaga taatcacaac ttcaggtggc ggaatgcttg tatctaatga
     7261 caaagaaaag gtggaaaaag tgagattttg ggctacacaa tctcgtgaga aggcacgcca
     7321 ttatcaacac aatgaaattg gctataatta tcgaatgagc aatatcgttg caggtatcgg
     7381 tcgaggacaa ttgaaagttt taaatgaaag aattgagaaa aaacgatata ttcatagttt
     7441 ttataaaaac gaattgtcta aattatcaaa tatacaattc atgccagaaa ataattggaa
     7501 tgaaccaaat tattggttaa gtgtcataac aataaaggga ataaatacga atcatcttat
     7561 tgatgagtta gataaaaata atatagaagc ccgcccagta tggaaaccaa tgcatttaca
     7621 acctgttttc aaaaattatg attatattgg cgatgatgta gcagaaactt tatttgactt
     7681 aggagtatgt ttaccatcag atacaaagat gacagatgaa gatttaaaat atgtagtatc
     7741 tacgattaaa agagtgatca atgatgcgta aaaaaacttt ctatcagctt tttgggaaaa
     7801 gaattttaga tatcctattg tcaggaattg ctctcatcgt tttatcgccg ataatcttaa
     7861 ttgtaggttt tttagtaaga attaaacttg gttctccaat tatcttcaaa caagaacgac
     7921 caggaaaaag tgaaaaaatt ttttccatgt ataaatttcg tacaatgact gacgaaagag
     7981 accacaatgg agaatattta cctgatgaaa ttcgactaac taaatttggg aaaatgctta
     8041 gagcaacaag tctggatgag ctacccgaac tttggaatat tttgaaaggt gatatgtcta
     8101 tcgtaggacc tcgtccatta ttagtggagt atttgccact atatagtgag aagcaacgga
     8161 aaagacacaa cgtaagacca ggattaactg gactggctca ggtcaatgga agaaatgcta
     8221 ttagttggga agaaaagttt gatttagatg tttattatgt tgataaaatt agtttcttta
     8281 atgatcttat tattataatt cagacatgta aaaaagtaat caagaaagag aatattaata
     8341 caatcaataa caatacccca gaaaaattta aagggagtta aatacagcag cttaatgcaa
     8401 gttttgtgac ttgcattata tttaggtata aatcaaagcg agtatattag aattatataa
     8461 catatgttaa aaaaacgcat agctaataaa agtatgattt taaaaatttg tgtagagttt
     8521 taaatatact gtttcaatac gagaacatta taaaaaagta taaggaaaag agagttatgg
     8581 caaagtatta taataaaaaa attcttataa tggcaaacaa cgatgatggt ctttaccgtt
     8641 ttcgaggaat gctcataaaa aacttagtag atcaaggtaa tgaagttatt gtatctcttc
     8701 caaagggcaa gaatatttct aaattaattg aacttggatg tcaaattatt gaaactccaa
     8761 tagatagaag aggaattaat ccaaaaactg actttaaact attttttaac tattgtaata
     8821 ttttgaaaaa aataaagcca gatatggtca ttgcttatac tatcaaacca aatatatacg
     8881 gtggattggc atgtcggtta agaaaggttt cttatgcatt aaatataacc ggattaggaa
     8941 cggcatttca aaaaaagggg gttttgaaaa gtcttattgt atttttgtat agattagcag
     9001 ctaaaaaatc taaggtcata tttttcgaaa attttgaaaa tatgaaaaca tttcagactt
     9061 tacaaattgc taaacaaagc aaatacaagc tattaaacgg tgcaggagta gatgtagaac
     9121 actttagtta tttagaatat cctaaagatg agcaagttca ttttttattt attggaagaa
     9181 ttatgaaaga gaaaggtgta gatgaacttt ttgaagcaat gaaaaactta tatagtgaag
     9241 agaaaaattg tgtacttgat gtccttgggt attttgaaga agattacggc ataaaaattg
     9301 aaaaatacga aaaagaggga tggttaaaat atcatggata tcaatctgat attcgtccct
     9361 ttgtagaaag agcacattgc tttgttttgc ctagttggca cgaaggaatg gcaaatacta
     9421 accttgaatc agcagcatca gggagaccaa ttattacaag tgatatccca gggtgtaaag
     9481 aagcagttat tgaatcgaag tctggttatt tatgtgaaag aaaaaatagt tgtagtctct
     9541 ataattcgat gaataaattt ctaaaattat catatgagca gcgcattaac atgggaaaaa
     9601 tggggagaaa ttatatgatt aaaaaattta ataagaagaa agttgtcgat gaaacaatta
     9661 aatcattatt tagttaaaaa gatatgtttt gcaaagtatg tgatagaaaa gagggattct
     9721 gtttgagtaa cgtaagtaat ccattagtaa gtgtaatcat gggagcctac aattcagaga
     9781 aaacaattgg aagagcaatt gattcaatta taaaacaaac atatttacat tgggaaatga
     9841 taatttgcga tgatggatca actgatggaa ctgtaaaaat aatacggaaa tatgtagaaa
     9901 aggataaacg tattaagctt gttcaaaata aaaaaaatag tggattagca tattcattaa
     9961 atcattgttt gaaatattca tcgggaaaat atgttgcaag gatggatgca gatgatatta
    10021 gtttaccaga aagatttgca taccaagttg gattctttga aaaaaatcca gaatatgacg
    10081 ttgtaggatg taatcgaata gtattcgatg aaaatggtga ttctggaata cgaaaatcaa
    10141 aggaaattcc aactattaat gacatgtata aaggttctcc atttgctcat ccgacaataa
    10201 tgatgaaacg aagtgtttat actattctag gtggttacag ggttaacaaa gatacaacaa
    10261 gatcagaaga tttagaaatg tggtttcgat tcttcaatga aggatttaga ggttataatt
    10321 tacaacgagt cttatttaaa tatcaagaaa caagcgaaga ttttcataaa agaagtttaa
    10381 gagcagctta cggaataaca aaagtattgt tgagaggtta taagttaata ggagttagta
    10441 aatataaata tatttacgca tttaagccga ttat
//
LOCUS       DAJSVF010000090        10420 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSVF010000090 DAJSVF010000000
VERSION     DAJSVF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.093"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            103..1146
                     /locus_tag="ON550_002317"
     CDS             103..1146
                     /locus_tag="ON550_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3955359.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1319..1543
                     /locus_tag="ON550_002318"
     CDS             1319..1543
                     /locus_tag="ON550_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955360.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1546..1803
                     /locus_tag="ON550_002319"
     CDS             1546..1803
                     /locus_tag="ON550_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955361.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2020..2895
                     /locus_tag="ON550_002320"
     CDS             2020..2895
                     /locus_tag="ON550_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955362.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2905..3699
                     /locus_tag="ON550_002321"
     CDS             2905..3699
                     /locus_tag="ON550_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955363.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3728..4846
                     /locus_tag="ON550_002322"
     CDS             3728..4846
                     /locus_tag="ON550_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955364.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4879..5199
                     /locus_tag="ON550_002323"
     CDS             4879..5199
                     /locus_tag="ON550_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955365.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5454..6143
                     /locus_tag="ON550_002324"
     CDS             5454..6143
                     /locus_tag="ON550_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HCR3955366.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6144..6347
                     /locus_tag="ON550_002325"
     CDS             6144..6347
                     /locus_tag="ON550_002325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955367.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            6367..7995
                     /locus_tag="ON550_002326"
     CDS             6367..7995
                     /locus_tag="ON550_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955368.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVIFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8046..8417
                     /locus_tag="ON550_002327"
     CDS             8046..8417
                     /locus_tag="ON550_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955369.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8502..8702
                     /locus_tag="ON550_002328"
     CDS             8502..8702
                     /locus_tag="ON550_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955370.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8836..10062
                     /locus_tag="ON550_002329"
     CDS             8836..10062
                     /locus_tag="ON550_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955371.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
BASE COUNT         4070 a         1263 c         2093 g         2994 t
ORIGIN      
        1 aacaacgatt atgatcttaa aacaatcgtt tatgtaattg gcaatcttac gaataatttt
       61 catgcaataa tgacaataaa aaatgaaaag gggtaaaatc aaatgaaaaa atttttagta
      121 ggaattctat tcacaatggg gctagtatta ttatcacctt cagtttcagc agaaagctct
      181 atttccatgt atcgtatgta taatcccaat agcggagagc atctatacac tagaagcgtt
      241 ggagaaagag acaatctgaa acgttatgga tggcattacg aaggtgtcgc atggcaagct
      301 ccaacaagtg gtactcctgt ttatcgtttg tataatcggt ataacggtga acatttttac
      361 acagttgatg ccggagaaag agataagatt tcacacacag gatggacgta cgaaggtgtt
      421 gcgttctatt cctacaacgg agcaaataga gttcctgtaa cacgtttata taacaaatac
      481 gtgaattggc atcactacac gttagatcaa aatgaaaaga acgtaattag taaacaaggt
      541 tggaagattg aaggtacagg ctggtatgca atgccaaaca gcgtaccaaa tcctacacct
      601 gctccaagca ctgaaaaagt gttgaacgtt cctgttgtat ggcaaggaaa tacaatgtta
      661 tgcgaaggag cttctttatt agaaggttta cactacaaag gtgtagccac aaatcaaagc
      721 ttaaccagtt ttgtaaacta tatgccaaaa gctaatgaca ataatcctta tcatggttac
      781 tcaggagagt ggcgtcataa cgttaatgga acgtatcaag gcatgatggc tgacccagta
      841 gttaaatggg caaaaaacgt tggcggtaat gccgtcaata tcactggttc aggaacgaat
      901 ggaatcaaga atgaaattcg caatggtaat ccggttgtaa cttggattac ttataactat
      961 gctacgccag agttcaaaca aatgccttgg ggtcgtgcag tatggaatgg acatgtagtt
     1021 ctcgtggatg gcttcagaaa tggtgcatac catgttgttg accctgtatt tggcgcaaaa
     1081 tggatcaatg caagcacgta tgagagagca tttaatgtga ctggaatggc ggttgctgta
     1141 cgttaattga tagaattatc aactaaatga aataagagct taattactga aatggtaaat
     1201 aggctcttat ttttttagag gaaatttttg taatacgaat ctataacaaa aaatattgaa
     1261 tgtagtacac aaaagtggta tattaaaatc gtagagatta tgcgaagagg tgattttgat
     1321 gaaccaaaga agaagaaaaa ggttagaaga aataagcgag cgactttccg agctaaagga
     1381 tgagttagaa gtaattcaag aagaagagta caaagaaaat atacctaaaa atctacaggg
     1441 atcagaaaga tatgaaaaag cagaagaatc cgttgatcaa cttgaagaag ctgttgatgg
     1501 tttatccgac atattagaat tgattgaaga tatagctagc tagagatgat taatgaagat
     1561 ttcaggagtg agaataaaat gattgatatt tcactgatag agaagctact tttttcaaat
     1621 aagtacacag ccaaagagtt ggctacaaga tggcaaatat ccgaatcagc tattaaagcc
     1681 tatcgtcaaa ataagggaag cgtaggatgg cgtgattgga aaggaatcag cattggaaaa
     1741 gcagaagaca taatgaaacg tgaaggattt caagctgaca atgattctaa aaaagttgaa
     1801 tagtttaata gtcgccaaga aaagtaccag gacgagggga aaggtagatt acaaaggaaa
     1861 acacttttaa ataaacttcg caaaagcatc aggttatgag tggctagggc tatacgaatg
     1921 tgatgaaaac aaagggcttt aatgtcaaag aaaacgcaat agggggggaa caaaattttt
     1981 aaaaaagata tttatgtttg gtttaatgga aaaaaatata tgactactaa caaaaggtgg
     2041 tcagagctag tacaaaatac agttaaaaaa gtcatgccag atggatatca tttcaactat
     2101 atacaatgct cacaaacaac tgaaagtatt tatttttcaa tattaaatga gaatagactc
     2161 tattatttca gggtaagttc acaccatagg aaaaagagag aagataatgt tacgacattt
     2221 ttatcgggaa aatacaatgg cttcaaagag ttaagtgaag ccattggtga gtatctaaaa
     2281 agtaatagat ttgtcagaat ttctgacaaa catatattga tcttgttact ttatttgaat
     2341 aatagcttca aattgttttg gagagacgac actttctact atattgagaa cggcatggat
     2401 atttattctg gtatcaacgg catattaaaa cattatatta aggcgttact tcgatatggg
     2461 gttctttatc aagaagattt aagtaaccaa attaagatta gtaattctgg aaagtcatta
     2521 ctggcaaaat ataaaaaatt gctttctcct atctacaaag gaatagatat ggaagctgta
     2581 aattctgaaa ctttatttaa tttagttgtt gatgaaaagt ttgcgaatga attttttaaa
     2641 agtaaaaaaa ttgaaagact aaataacctg tttcctgaaa atgcaataaa agcttatgta
     2701 gataataata aatcacgatt atacaactgg tttattccca aacggttgcg aggaaaaatc
     2761 aaaattggcg atcaagtgaa agtaattaat ctaaatcata ctaagatcgt taccgtaacg
     2821 gagttgtata tagctgaaaa agaagatatt acaaaaatga aaccagttat taaaatacta
     2881 gataacaaag agtgagtgga aaatatggta gcaaaaaaga atactctaaa agaagcttct
     2941 gaaaaatatc aagatatgaa aagacgagga ttaataccaa aggacaaaaa aggtatatgg
     3001 agagaatttg ttaaggaagt cagtagtgat cgaagtggcg gtgactttgt tggacgatat
     3061 acagcaaaag gttatagcag gcgcttaagg atcactgttg aatatggaac gaatgaatca
     3121 gctacagagc tgaagcaaag gtacttagat acacgaagag aagcttcaaa aaaatatgat
     3181 gatttcaaga gcagaggtct aattcctaag aagaaaaagg ggatttggcg tgagtttttc
     3241 aaagaagaag atagcaatcg gaataaggga gacttcattg gacgatacac agcaaaaggc
     3301 tacactaaga atttagggat caaggttgaa tacggaactg gtgaaagttc atgggaattg
     3361 agaaatcgct atttaaatac attgttagag aaatataaag cagaattatt ggagttgttc
     3421 gatgttaatg attttatcga gatggctgaa aagtatggtc atgaagaagg ttttatgatc
     3481 aatgacatta tggttattga taattttacg gatttattag cacttgcgaa aaaagcaagg
     3541 gatttaagag aaacgcagat gtatctattc agaaataaaa acggaagaaa ccggagaact
     3601 gaatcgagtg agattgaaga ttactatgat gcctttacac acgatctggg agcctttgta
     3661 gacgagataa gtggtgtaat gaacaaatat agggtctaga ataaaaaagc cggagatgag
     3721 cagataaatg ggaaacaata gcatatacac acctgaaatg cagaataaaa ttaaaagact
     3781 tgtcggatta gggtggatga caagaggaaa attagattct tttagtacga tcaaatatct
     3841 tttgatggac atttaccgtc agaacaagca aacaggaata cattttttgg gctatgataa
     3901 gccggaagat gcacctttct ttgttccaag ttcagcctat agagccagta cctccggtag
     3961 catgtataac ttggatttgt ttgaggaagg agcaaagatg atcgaatatg atttcaacga
     4021 agcctattcg aggatcatga gaaattatcc tttgccgtca aacacttatt tgaaagcaaa
     4081 atataataca gatcaagcta ttttgagagc gataaataga ccaaaagtaa gaccgtatga
     4141 aaatttaaga acgtatatgt tctttttggt taattttgaa ggaacagcca aagaaggaac
     4201 atatgctaat ccaaaatcac agtcgatgtt tgcggattat ggcgaaaatc ccaaaacagc
     4261 taatgagcct atttggttat cggagattga gctaacactt atccttgatt attatgatat
     4321 taaagagatg gaagtcctag atagccacac atttttgacg aaaaaaggat tactcaaaga
     4381 atattttgaa agaatcgagc cattaaaaac tgatcctgat acgttgaagc tttataaaaa
     4441 tcttagaacg aaagtatttg gtgctatcgg acaaaaatca ttaagagaag gagaaacaag
     4501 caccttcgat aaatacagta tgtataatcg agcgtattcc tccatggtag caggcgtatt
     4561 tagagatcgt atggttagat atgaacagaa atacgttcat agcgcatatg ggctattaaa
     4621 aattcgtact gatggattgt atttcagatc cgaagtacca gagtttgaag agctgtataa
     4681 acttggagta gttaagaaaa aagttcatac aatatccaaa gaagaagtcg aaaaagcttt
     4741 agaatttaaa aacaaaggat ttgaaaataa gggctccttt gggaaatcta aagatgaagt
     4801 taagggtgtt gtgatagacg atgatttcga gtttgatttt gactaaatac tctttggaaa
     4861 agatggtagg aggtaaatat gaagaagaaa attgtggaag gaaatgtagg tgttattatt
     4921 ctaaaagatt caactgacta tgctaaatca atggcactaa aagacgaaaa agttgcacgc
     4981 atggataaag atttagttga atttctgatg gatgatacag aaagattaaa aggggatcag
     5041 tttttgagtt taatgaaaga caaaggctta aacacatatg gtttatttga ttataagaaa
     5101 ttagctatag agtgggtaaa agaaggagaa atatttttta ttgaagaatg ggaaggtact
     5161 gagaacttag ttcttcatca gtatcttttg aaagcttaat ctaagtaaaa cagttctgac
     5221 tagttatgct tgtcagaatt tcaaaggaag ttttccataa ccatcatcat tcaaaaataa
     5281 aggatttgaa aataagggtt cctttgggaa atctaaagat gaagttaagg gtgttgtgat
     5341 agacgatgat ttcaagtttg attttgacta gatttttttg caatgtttaa gagtaaacaa
     5401 ggtaagaact aagatcatga agattgagag taaagaagat agaggacgaa tgtatgagaa
     5461 cacactatat ttatttagaa gataaaattg tagatgaaat tccggcaaaa agtcgagaac
     5521 aagctagaaa attatttatt gaaaaataca aagagatcaa tggagatagt gaagttccaa
     5581 aagtggtcgt aacagttaga aaaaggcaaa caaaaaagag aagtacgttt catgtcagcc
     5641 ctgatatata taatcttata gagcgacata gaagtgaaga taatatttct atctctcaat
     5701 atattgctac agttattgat gaattgattt caggaaagta tgactatgct tttgattttg
     5761 agggtgaaaa ggattacaaa agtgttgctg tctttcaatc tgattttgag aagctaaaaa
     5821 actatgctgg caggagaaat gttaattttg caagactact agaagagttc tactccgaag
     5881 agttgggagt aggaaatgaa tacaagagag aaacttatgc gaaattgtct cataagttat
     5941 cccaagcgct taaagaagtg acagaagtta aagcggaact agatgctaaa cttgaagaag
     6001 tgaaaaaata tcaaacagag aggaagaaca acatgaagaa tcaagtggaa tttgacgaac
     6061 agaaagaatt tgaaaaagca agaaaacgct ataacaaaat tatgaatgaa cttagctatg
     6121 aacacttaac agttcatgaa taattgagtg agggtacaga aaaatatacg atcgcagata
     6181 tggttaatga agtggaaact cttttagctt ctttctttga aaatggtcac gtaaacgatg
     6241 aatggcgaaa agaagaacct aaaagggtta acgcttatat tgctcgatta aaaaggttta
     6301 ttgataaata taaggagaat agtgacaaat tttctaaaga taaataagga gagagtgtga
     6361 ttacatttgc cagaaatcga aggaatattt tcgccaaaaa taggtaaaaa acagttagat
     6421 ttcagcagtt ttccgagcgt aaatagagat tataatctag gtgatgaggt acagatacta
     6481 aatattgatt tcaaagaact agacttcgct gttgctgttt ccaacaaacc caaggcattg
     6541 gaacgagtga aaaaaatata ccaagagaat gatcaaaata tcaaaaatat gatcaaaaag
     6601 tatagacgag ataattcaaa agttgaagac gttttattca acggtacggc aaaacatgat
     6661 ttgaatgttt atatggcatt aggagtggct ttgtactgta attcaattaa tcagacagat
     6721 attttggtta atatcgtgag gaatagctat caaaaaatat taaaaataag tagcgaaaca
     6781 aacccttatt atgcaatgaa atatcttggg gataatttat taaatgataa aaaattgatg
     6841 cacaacactg aagagctgtt atctaatttg gctgcgatta actttctatt caaaacaggt
     6901 tctgaagatt attttataga taacgtacta aaaggtgctt acggaacagc attttatccg
     6961 ctttacaata tgaaacagca taaagccgaa caaaaggata acaaacttga aattaaaaag
     7021 atgaaagaac tattaggtat aggatttgta aaacccaatt caaaagtcac tattgcttcg
     7081 ttagcaacta ctaacaatga tgggaaaata gtaaatgggt ttgacgattc agatatagat
     7141 atattgtcaa aatcatattt ttctttgtat ggagattttg cagatttgat gggtattgat
     7201 agttacttag tcaataattt tgagatcaag ggtagcgagc tgaatgatat tgtgtatttc
     7261 gttttaatct ctctatatgc aattgatcag aataccgagt accgacaaat tgactatttg
     7321 ctagtttttt atagtttgtt atatcagttc gttttaactc ggttgtttaa aaatgctaag
     7381 gaagaattga tcctgaattc tgattatgaa atagcaaaac aaattgcaag tattagaaaa
     7441 gaaaacaaat ctgaagtaat caaattaaga aaagaattaa gtattgcgag taaagaaaac
     7501 ggtcggttag aatctttagt caaatcggca aaaaaagaag aggaactttt gaagcaagag
     7561 attaaagaat taaaaggaat cagaaaagaa cttgaacagc tgaggtttga aaataaagta
     7621 ttaaagcaat tgcaggagca agaacataga caatcattaa atggtaaaac tgaagagcct
     7681 tcactgtctg aaatgatagc ttatctaaaa cataaaaaca ttcttttagt gggaggacat
     7741 gtaatttttc aaaataaatt aactgaacta ttaccaaata ttaaaatgtt tgatgtaagc
     7801 gaaaataatg gaaagatact taaaaatttg aatacgtgtg atgcgatttt tctatataca
     7861 gattacttga atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag
     7921 atattttatt tggataatca tgtgaatatg aacttaacaa ttaaacgtat ttacgatctt
     7981 ttatcactta tataaagaga gaagtgataa ataggacaca aaatttggaa agtaggtaag
     8041 tcactatgag agaactaact ttatcttcta aggaattaga aaagaaaact gctttattaa
     8101 acgaaatatt tagtgctgat tgtataatcc aaagtttaaa agaaagttca ttttcaaaac
     8161 taacagatca ggaaaaaatt tcttatatgc taagtttaga taatctagga actaaatatg
     8221 atgaatcagc aagtagcgat aagcttttga ttgaattagt atcaagtctt agtagttttg
     8281 gaaaaatgaa aagatttaat gtgatattag aaatttttgc ttggttaatc ccttcattta
     8341 tttggttttt ttatattttc atagttgaat ctgagttaat agccaaggtc tctcttggac
     8401 tgttaattcc gatataaata ggttcagcaa tttactttaa taacttgtat acacgtattc
     8461 aaataaaaaa gcagttatta catgaaggat taagtggtaa tatgttaaaa agaattgcat
     8521 gggataaaga taataaaaca gatattgagc ttaaagaata taaatacaat tatttctcga
     8581 gatgtataga tagaaagtta ctgtttaaaa aagaaggatt agaatttagt tttgatgaag
     8641 atgaatttga aaaattagtg atggactggc gtaagaattt actggatacg gtacaattct
     8701 aaaagcaatt gaggtcgaaa ttccgatact tgaatcatga tctatattat cgtgttatga
     8761 gtgaaatttc taataaagat aagatatttc atttggacag tcatgtgaat atgagcttaa
     8821 caattaaacg tatttatgat cttttatatg tttataaaca ttaatgaggt gctaaaattg
     8881 acaacaataa ctatgacaaa aaaaatagaa gtatatgttg atggatcaca agaagaaagg
     8941 gattcctttt ttgatttttt ttggaaaaat agtagagaat atgtaaaagc gttgaatctg
     9001 gtatataaac agctaatgtt tgaagagttg ataaaagatg caattaaaga aatggatcaa
     9061 ggttatgctg agagagaaga aagtattcaa aatcggattg aaaagttagc atatactgat
     9121 ccaatagatc aaaaaaaact tgataaagca aaagctgaat tatctaaatt gagaaatgaa
     9181 aaaaataaag aagcgagaga aatattaacg caagcgatag ggttgaagag tcaaacgaga
     9241 acacgagatg tagcaaaaaa attagacttt gactttggag attttgttga ttccgcaaat
     9301 atgaaagcta gtaaagattt tggcaatgat tttatcggaa ttgtgaccgg acaaagaacg
     9361 cctagaatat ataaaaatag aaacaaatat ggattactac cttttagagg tcgagatatt
     9421 aaaatccata aagatgataa gttttatatt tatttaccta aaggctttag gtttaaagtg
     9481 aatttaggta gcaagccaag aaaagcaaga gatgttgctt cagttttaca acacattatg
     9541 gatggcgatt acaaattaaa tcaaagtaat cttatttttg atggaagaaa attatttttt
     9601 aatctaacaa tttcttttga aaaaagctat aatgaaatgg aattcaagaa aggtaatgaa
     9661 ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt taattaatga taaatctgta
     9721 atgtcttttg gtgatagcca atatataaga aacttcaaaa gtaaaatttt agcaatgaaa
     9781 tcaaaagaac aaagccgagg agtcttttct aagggcggtc atggtcgaaa aagaaaatta
     9841 gatcaagatc gatgggaagc aataaagaaa agagaaagta attttgttaa aacttataat
     9901 aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca ataaagctca aattattcgt
     9961 atcattgaac cagataaggc ttatggcgaa tgggcttact atcaattgaa agaacaacta
    10021 gtaaacaaag gaaaaagata tgggatagaa gtcagaagtt agtaaattaa acaatcgttg
    10081 atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc cgtcaagttg
    10141 tgtgaccaga cggaaggacg aatgttgttg aggttttaga accatataaa acagcatgtt
    10201 tctcaaattt ttaggataag ggaaattgct agcagtttgt tttagaacca tataaaacag
    10261 catgtttctc aaatgagtac tttaaattaa ttgatggcgt tcctgcttta gaaccatata
    10321 aaacaacatg gatttaaaac aagaagtaag tcagttctcc ttttacttta gtaaggaaaa
    10381 ataaagtatt ctcaataaaa cttaaaataa gcaaaccttt
//
LOCUS       DAJSVF010000091        10203 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSVF010000091 DAJSVF010000000
VERSION     DAJSVF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.094"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            117..266
                     /locus_tag="ON550_002330"
     CDS             117..266
                     /locus_tag="ON550_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR3955372.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            277..1791
                     /gene="dltA"
                     /locus_tag="ON550_002331"
     CDS             277..1791
                     /gene="dltA"
                     /locus_tag="ON550_002331"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR3955373.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1788..2993
                     /gene="dltB"
                     /locus_tag="ON550_002332"
     CDS             1788..2993
                     /gene="dltB"
                     /locus_tag="ON550_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR3955374.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3038..3271
                     /gene="dltC"
                     /locus_tag="ON550_002333"
     CDS             3038..3271
                     /gene="dltC"
                     /locus_tag="ON550_002333"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR3955375.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3274..4542
                     /gene="dltD"
                     /locus_tag="ON550_002334"
     CDS             3274..4542
                     /gene="dltD"
                     /locus_tag="ON550_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR3955376.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4683..5765
                     /locus_tag="ON550_002335"
     CDS             4683..5765
                     /locus_tag="ON550_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR3955377.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5765..6568
                     /locus_tag="ON550_002336"
     CDS             5765..6568
                     /locus_tag="ON550_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3955378.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6638..7378
                     /locus_tag="ON550_002337"
     CDS             6638..7378
                     /locus_tag="ON550_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3955379.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7389..8393
                     /locus_tag="ON550_002338"
     CDS             7389..8393
                     /locus_tag="ON550_002338"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR3955380.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8438..10135)
                     /locus_tag="ON550_002339"
     CDS             complement(8438..10135)
                     /locus_tag="ON550_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955381.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3273 a         1694 c         2072 g         3164 t
ORIGIN      
        1 ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct agacacatgt
       61 ttctttttgt caaaattagt tacactttgt gtgaggttat atgaggaggg aatccaatga
      121 acaaatggcg ttctaatcca aaattgaatt attggggaaa attcatcatt cgaacaattt
      181 tttatagtgc agtactgctg ttcctgattt atctgtatca ctataaaaat attcaaggag
      241 gaacttttat ctataacgaa ttttaggagg aagagtatgg aaatcaaaac gattattgaa
      301 gcaatcgatg attggggaat aaatgaacct gatcgagttg cttatcaggc ggatgagacg
      361 catacttttg gtgaattgaa acaagcatca gacgcacttg cttattattt agaaaaaaag
      421 gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg agtttgaaat gattgtctca
      481 tttctagggg ttgtcaaatc tggccatgcc tatttgccga ttgaagagca tacacccaag
      541 gagcgtatcc tatcgatctt tcgggtggct aaaccatcta tggtcatcag tatcggggac
      601 tggatagaag atattccttc ggttcctgtc ataacaaaaa aagaatttca agaaatcaag
      661 aagataccag taggatttta tcccagaaat agcgtaaaag gtacagaaaa tttttatatt
      721 atttttactt ctggtactac tggagaacca aaaggcgtac aaatcagtca tgataatcta
      781 gtcagctttg ttcaatggac gatggaagac tttgggatca agcctggtat gcatttctta
      841 gcccaagcgc cattttcttt tgatttatcg gtattttcta tttatcctgc attagtttct
      901 ggcggaatgc tgaagccgtt aaataaagca gttgttcaag actttcgtca attattcgcg
      961 acattgccag ccttgaagtt gaatgtgtgg gtttctactc catcatttat ggatatttgc
     1021 ttgatggaac ctacattcaa tggagaaaat gtgccggaat tggacatgtt tttattttgc
     1081 ggagaagaat tgacgaaaaa aactgcagaa agtctacttg atcgattccc aaatgcacgg
     1141 atatataata cttacggtcc aacagaagca actgtagcta tttcaagtat tcagattgac
     1201 cagcatgtac tagatagtta tgaccgacta ccgattggct acgtaaaaaa agacacgaaa
     1261 gtttctattg tccaagaagg taaacctgtt tcaaaaggag aaacgggtga aatcattatt
     1321 gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg aaaagacagc tgctgcgttc
     1381 ttttgtcaaa atgacttagc aagttaccat acaggggatg caggtagact agatgaatat
     1441 gggcttttat tttatgaagg acgtatggat cagcaggtca aattacatgg ataccggatt
     1501 gaactcgggg atatcgaaca ttatctgctc caagacaacc gaatcaaaca agctgtcgtc
     1561 gtaccaaaat accaaggaac aaaagtacag cagctggttg cttttgtcgt attagaaaac
     1621 aaaacgaaac agccggattt tcagttgact aaatctatca aagaacaatt actagaagtt
     1681 gtaatggatt atatgattcc tcagaagttc cattatgtcg atctgctgcc tcagacagtt
     1741 aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg atgttacctc
     1801 atatgatccc ttatgcagag ccgttttatt tttttctttt agccattgcg cttgtaccga
     1861 tcgttctatc tttattgata tgggaaaaaa gattaccagt ttatcaatca gcagtgacac
     1921 ttttcttttt atttgtcagc tttggaggta attattggca acagggagcg gcgctgattg
     1981 ggtatgtttt gtggcagacg atacttgtat ggggctattt tgtttatcgg caaaggaaaa
     2041 atcagacagg atggtttttt gccgccgtta ttttagcgat aattccactg tttttaacga
     2101 aagtgactcc tttttttgaa catggaaaag catcgttact tggtttttta ggaatctctt
     2161 atttaacctt taaatctgta caaatgatca tggaaataag agatggcatg atcaaaacat
     2221 ataagccatt tcgttatatc cagtttctgc tgttctttcc aacaatctct tctggaccaa
     2281 tcgatcgcta taggagattt gaaaaagatc agctccatcc gcctgttcgt gataaatatg
     2341 ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg ttttttatat aaattcatta
     2401 tcggttatta ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc ttacagcatg
     2461 gtggtatttc atgggcatta gtaggttata tgtacgtgta cagtatgtat ttgttttttg
     2521 attttgctgg ctatagctta tttgcagtcg gtacaagcta catgatggga tatcagacac
     2581 caatcaattt caataaacct tttttatcat ggaatatcaa agaattctgg aatcgttggc
     2641 atatgacgct gtccttttgg ttccgtgatt atgtatatat gcgattgatg ttcttcatga
     2701 tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa tatcggttat tttgctttgt
     2761 ttcttctaat gggtatatgg catggattga cgtggtacta catcgtatat ggactgtatc
     2821 atgcttgtct gatctgtctg acagatgcat ggatccgatt taagaagaag cataaagagc
     2881 agttgccttc taacaaattt acgcatggct tagccgtatt tttgacattt caagcagtat
     2941 gttttagctt cctgattttt tcaggatttt tagataaact tttttttagc taacgaatag
     3001 tgacatatta atttatataa caaaggagaa aaggaaaatg gaagaacaaa ttttatcaat
     3061 cttagaagaa ataacaggaa cagatgaagt aagaaaaaat cgtgatattg atctatttga
     3121 tgaagggttg cttgattctt tagcatctgt tcaattatta gtcgagctag acggagagct
     3181 ggatatccaa gtccctgtat ctgaattcga gcgagaggat tggagcacac cgaataaaat
     3241 cattcaacaa gcaactgcat tgaagggttg agtatgaaaa agaaactttt tgctattttc
     3301 ggcccggttg ttttagctgt tggcttactg tttgtgtttt ttacctcctc ctatcaagta
     3361 gatatctcta atccagaaac gattagacgt gcctcttctt ccatgtcagt gaacgtgttg
     3421 aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac aatatattcc tttttttggc
     3481 tcaagtgaat taagcagaat cagtccattt catccgtctg ttctggcaga aaaataccag
     3541 cgaaactatc gtccattttt gctgggagca cccgggacac aatcgttaag ccaatatatg
     3601 atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat tatttctcct
     3661 cagtggttcg ttaaaaatgg tgtaaaaact gattacttta atacgtatta ttctgaactt
     3721 caaacatacg actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg ctatttagct
     3781 agaagattat taactttctc aaaagtaaaa gagaatgata cgttgacggc gattctgcaa
     3841 acgatcaaaa aagggaagct acctttacct gaatctttga atcagctgcg cagccaatgg
     3901 aacatgctga agagagagga tgaagtattc agcaatattg ggttgaaaga tcgacagcaa
     3961 aaaatcgatc atgagtcaaa acgattacca aaacaatacc aagaaacaga attatccatt
     4021 ttagcaaatc aaattggaga acgagaaacg acgaataatc cattcgggtt gaaaaacgat
     4081 ttttatacgc atcgtatccg agcccacgag ccagagctca aacagtcaca aaaaaattgg
     4141 gattatcgtt tttcgccaga attttctgat tttcagctgg tcttggatca attagcaaaa
     4201 aaccataacg aagtattgtt catcatccca ccggttaacg agaaatggag cgattatacc
     4261 ggactatctc aagaaatgtt acaaggattt gccaaaaaga tcaaatttca attaaatagc
     4321 caaggattca atcgaatagc tgattttgtc aatcaagcgg gtaccaacta ttttatggaa
     4381 gacacgatcc atctaggctg gaaaggctgg ttggctgcag atcaacagat cagaccgttt
     4441 ttagaagaaa atcatattac tgctagtaag tatcatcttg acgatgcttt ttttagcaaa
     4501 tcttggcaac atcaaatacc agataaacta caattaaaat aaagaaaaca ggcgagtgtt
     4561 gctcgcttgt tttctttatt tttgaatatt ctgaataaaa gataaaaata ccaagtttgc
     4621 ctgaacaaaa atcataagca atggtataat cacaatatga atatagaaat gggggaaaac
     4681 atttggatac aaaagaagaa cagctactta ttgatttaac ttctgcaaaa ggagtacctg
     4741 gaaatgaaga agaggtaaga gaagtattca gagaatatgc caaaccattc gctgatgata
     4801 ttttttatga cggtttaggc agtgtgattg ccaaacatgg tgctggcgga ggccccaagg
     4861 tctttatttc tggacatatg gacgaagttg gttttatggt aacaaaaata accgaaaaag
     4921 gattcttgga attccaaact cttggaggtt ggtgggggca agtcatgctt gctcagcaag
     4981 ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt gattggttcc aaaccaccac
     5041 atgttttaac acctcaagta agaaataaac cttatgaaat aaaggatatg ttcattgata
     5101 ttggcgcatc aagccaagaa gaagccaaag agtggggcat acgtcctgga gatatggtca
     5161 ctccatatat tgaatacaaa cgaatgaatg gcagtaaata tttactggca aaagcatggg
     5221 ataatcgtat cgggacagct gtttctctgc gagtattaga gaatctatct aaagaagcac
     5281 acccaaatgt tttattcgca ggaagcgatg tgcaagaaga agtaggcttg cgaggtgctc
     5341 gaacaagtac acaccttgta aatcccgata ttgcttttgc tttagatact ggtacagcag
     5401 gagacactcc aggaatgaca ccaaaagaag cagattcgat tttaggaaaa ggcccacaga
     5461 ttttgatttt tgatgcatct atgatcccgc ataaaaaatt attgaatttt gttattgatg
     5521 tagcagagga aatggatatt ccattccaat atacagtgat ttctggcgga ggaactgatg
     5581 caggacaaat gcatttgaca agagatggtg ttccgtctgt agccattacc gttcctgttc
     5641 gttatctgca ttcccataca tcagtgattc atgaagatga ttatttgaat acagtgaaac
     5701 tagtcacaga attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt cggagttatg
     5761 aataatgaat aatagaatac ctatttctga atatcggaaa aaagcacgag tttctttgga
     5821 aaatcaatgg ggaatcaata cttggcttgt tttcttatct ataatattta cgagcgtcat
     5881 ccagaacttt tttgacggtt tcagtccttt tccggaagaa agcatgcagc agcacttatt
     5941 gagttttcta gtcgataatt tacttttatt cgcattcaac tatgcattgt tttacattgc
     6001 gctagtagtt gtacggggtg gcagaccaaa aagtaacctt ttattttcag ttttccaaag
     6061 aaagtatttt ggttcgatac ttggcatcaa tttgatcaat tcagtcatca actggctact
     6121 caacagttta atctttctgc ccatgttttt aatcagcgga gtgagtactt atatgcagct
     6181 gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca gaagattttc ttgatatcag
     6241 ctttgtttta tcgatgatag ggatgatttt tgtttcagca ctcatcatga gtattgttgg
     6301 cggtttattc caatttgctg catggacaaa gttcgactat cctgatttgt cggttattca
     6361 atgtttgaaa tatggctggt ttttattgaa agatagaatt ggtacgtata ttttgttgca
     6421 gctgtctttt atcggctggt atttgctagg agcaatcgct ttgttcatcg gtcttttgtg
     6481 ggtcattgtt tatgtgaatg tgtcagttgc agaattttat gaacaagcac ggattgaaaa
     6541 aggaggtcca gacgcctatt ttacttagca tgtaagcagt ttattcatca aaaaaataac
     6601 ttttgtggta ggataaatct ttgaggggga atgtacgatg aaattaactg ttttaggatg
     6661 cttaggagcg tatccttata aaaatcaagg gacaacaggt tatttgcttc aatctgacgg
     6721 gttcaatttg ttgatcgatg caggtagtgc tacgctgatc aaattacaag aacaccttga
     6781 tccacttgat ctagatgctg tgatccttag ccattatcat catgatcata ttgctgacct
     6841 tggtgtatta cagtattact ggcaacttca tcctaagaga aatccggaga cattgctttc
     6901 aatttatgga cacacaaaag attccattca ctttgaagaa ttgacgttgg aaaatgtctc
     6961 aaaaggacat ccttattttg aagctgaaga attaaaaatt ggtccttttc tgattacttt
     7021 tatggaaaca atccatccag tagtatgtta cgccatgcgt atcgtagaag aaacaacggg
     7081 gaaagtattc gtatttacag gtgattcagg ttatttggaa agtttcgctg attttgcgaa
     7141 agatgctgat ttgtttctag cagataccta tctttttgaa ggaaatgaac atcatcatgc
     7201 ccatttcact tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa agaaacttgt
     7261 acttacacac ctacctcagt ttggtgattt agaacaattg aaaaatgaag cgcaaaaggc
     7321 agcaggcaaa gggattgata tagaacttgc tgcagttgac aaagtgttta acatttagga
     7381 ggaagaacat gatttttgta ccaaatgaaa accacgaccc aagaatcaat ttagcaattg
     7441 aacaatattt gctgcaagaa atgcctttag atgaacctat tttgctcttt tatatcaatg
     7501 aaccttcaat catcatcggc agaaaccaaa atacgcttga agaaatcaat cgagattatg
     7561 tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg aggcggagct gtctaccatg
     7621 attttggaaa cctgaacttt agttttatca tgcctgatga tggggattca ttccgtgact
     7681 ttgcgaaagt aacacagcca atcatccaag cacttcatga attgggtgtt gctggggcgg
     7741 aattgaaagg ccgaaatgat ctagtcattg atggcatgaa attttctggc aacgcgatgt
     7801 atgctaccaa cggtcggatg tttgctcatg gaacaattat gttcgactct gatatcaatg
     7861 aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga atcaaatcga
     7921 tccgttctcg tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa aaaatgacaa
     7981 cagaagaatt tcgtcaagaa atcttgttga aaattttcgg cgtagagtca gtagaagaag
     8041 taaaaacgta cgaattgact gatgaagatt ggaaaaaaat caatcgaatc tctgaggaat
     8101 attatcgaaa ttgggattgg aattacggga agtctccaga tttcaatttc tctcgccaaa
     8161 aacgtttttc tatcggttcg attgaagtcc atttgaatgt ctcagaagga atgatcaaag
     8221 aagcaaaaat ctttggcgat ttcttcggac taggagatat ctctgacgtg gaaaaaaaat
     8281 tggttggaca gaaatatgat aaagcatctc tatcaaaaat agtcagccaa attgatgtga
     8341 agaaatattt tggtgcgata gaaccagaag aattgcttac cttactttat tgaaaataaa
     8401 aaatagcgga atccacatat atggattccg ctatttttta tacaggattc gggtaatttc
     8461 gagagagttg aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt agtgttcctg
     8521 tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa acgttcctga tcatctaatt
     8581 cttctgtgat gcctcgacta aataggtttg tatcgtaatg tgcactatgt tcgcaacaaa
     8641 tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac agtggatttt
     8701 cgatggcatg agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt tgagccagat
     8761 cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg gaaggttatc tgaatattcc
     8821 gtttggcagc taacttctgg taacgcgcta agataagatt tgtcacctgg tggttgcaaa
     8881 aagaacacag catttcgaaa ttttttgctt cttctaatat cttttccaca tagtttttag
     8941 cttttgtttg gtccgtatgc attaaatctt gcagattctc catctcttgg acaaattcca
     9001 ggcgaagtgt atttacttct tggatatgct gtcgaagctg ctcataatgt tgctccaaca
     9061 aatcattttt gatttttaag aaatctactt gtctccgatc tttgtcggta tttaagatgt
     9121 gatcgattag attgtagctg ataataacga ttagaatgat aatatataaa atcgtagacc
     9181 aattgatcgt tgtttgatag atacctaatg ccgtttggat gtaaatcaag caatggataa
     9241 tggctaccaa gacgatccaa ggtgtacaag ctacataaaa acggttattc ttatatgctt
     9301 cgccaatgca tgcaagtgaa gtgactaaag tgcttataac actagcaata ttagcaatcg
     9361 gcattaattc aggaagttct gcaggagtaa aagttgcata aagcagaacg ccgataaaaa
     9421 tcaaaagttg gaacgtacaa aaaattccgt accatttttt atagtaaaac attcggctcc
     9481 aatagtagca gatgatgaaa gctgacgtta agataataaa taaatgagat aaaaaagaat
     9541 gaacgaatgg ttcaaaaaga ataccactta aaatatcttc tgatacagct tcaaatccta
     9601 atgccaaagc aaagcaactt aagagaataa gtgagtattc aagacgtttt tgtttataca
     9661 tcaagtagat cgtatagaaa aaggtcccta tagcgataaa aacagcgatt atcatcgtca
     9721 atacttgcgg cactgattgt gaataaataa aactaatcaa tggtccagtt gttcctagat
     9781 aaactttcgg tggcagtcca gcatagttct gataaggaga tgagactgta agggtcaatt
     9841 tttttccgat ataatttgct ggtaaatcta tcacagccaa tgagagtccg ggattgcttt
     9901 cttcctgata atctttccgt tggtataaaa tcttttcgcc tagatataca gtaatccatt
     9961 gatggtttcc tttgattagt aaatttgctt cgtccaacgg tttttcaagt gtcgtttcca
    10021 taatgaatgt ttcgtcttta tccacggtag gaatcgtacg agtgtacttg ctgtcaaact
    10081 taaaatcagg atcatctttt gtatagaaaa cccacgaatc ttttatttct ttcatttgat
    10141 tgctgtccgc aggagaaaaa ataaaataaa agaaataagc aattcctgtt aaaaataaca
    10201 atg
//
LOCUS       DAJSVF010000092        10136 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSVF010000092 DAJSVF010000000
VERSION     DAJSVF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.095"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            120..626
                     /locus_tag="ON550_002340"
     CDS             120..626
                     /locus_tag="ON550_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR3955382.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ON550_002341"
     CDS             643..1542
                     /locus_tag="ON550_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR3955383.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ON550_002342"
     CDS             1629..2513
                     /locus_tag="ON550_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3955384.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ON550_002343"
     tRNA            complement(2705..2776)
                     /locus_tag="ON550_002343"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ON550_002344"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ON550_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR3955385.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ON550_002345"
     CDS             4319..5410
                     /locus_tag="ON550_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR3955386.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ON550_002346"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ON550_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3955387.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="ON550_002347"
     CDS             6562..7752
                     /locus_tag="ON550_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR3955388.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="ON550_002348"
     CDS             7856..8611
                     /locus_tag="ON550_002348"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3955389.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ON550_002349"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ON550_002349"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR3955390.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1869 c         2100 g         2619 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc taaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagctg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggtggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcaaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgcggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 atttttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgactcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gatggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaatgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag agtttt
//
LOCUS       DAJSVF010000093        10091 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSVF010000093 DAJSVF010000000
VERSION     DAJSVF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.096"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            6..1214
                     /locus_tag="ON550_002350"
                     /pseudo
     CDS             6..1214
                     /locus_tag="ON550_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1214..1624
                     /locus_tag="ON550_002351"
                     /pseudo
     CDS             1214..1624
                     /locus_tag="ON550_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            1726..2025
                     /locus_tag="ON550_002352"
     CDS             1726..2025
                     /locus_tag="ON550_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR3955391.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            2015..3691
                     /gene="mdcD"
                     /locus_tag="ON550_002353"
     CDS             2015..3691
                     /gene="mdcD"
                     /locus_tag="ON550_002353"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR3955392.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            3695..4288
                     /locus_tag="ON550_002354"
     CDS             3695..4288
                     /locus_tag="ON550_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR3955393.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            4312..5388
                     /locus_tag="ON550_002355"
     CDS             4312..5388
                     /locus_tag="ON550_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR3955394.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            5413..6282
                     /gene="mdcB"
                     /locus_tag="ON550_002356"
     CDS             5413..6282
                     /gene="mdcB"
                     /locus_tag="ON550_002356"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR3955395.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            6393..7157
                     /locus_tag="ON550_002357"
     CDS             6393..7157
                     /locus_tag="ON550_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR3955396.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            7646..9739
                     /locus_tag="ON550_002358"
     CDS             7646..9739
                     /locus_tag="ON550_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3955397.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            9764..10066
                     /locus_tag="ON550_002359"
     CDS             9764..10066
                     /locus_tag="ON550_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3955398.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         3377 a         1728 c         2100 g         2886 t
ORIGIN      
        1 tatatatttg gttattaaat atttgtacga ttctgattag tttttattat ggagaaaagt
       61 tttttaaaaa gatagatgct gcttttttat ttagttgttt atatactttt gcaacttatc
      121 gaacagttga tatctattat cgatccgcaa ttgcagaagc aatcgctctt acgattattc
      181 cagctgtttt atattatgct tatggattga tattcaatag agaaaaaaaa gtcatcgctt
      241 tatctgtcag tataagtcta cttgtgtata ctcatgtctt aactacacta ttgtgtagtg
      301 cattgattgc ttgtatgatt atagttcgtt tgtttttaag caagcaaata aaaaacgata
      361 taaaatttgt ggtcgtgaat ttagtaaagg ctgttttttt gacttgtgca ttaactagct
      421 atttttggta tcctatgttg cagcagatga attatcaaga aatacacagt cccttcaaaa
      481 cagatttgca gctagaagca ctttctattt cagatagtat cattcaagcg ttaaataacg
      541 atattacaac atacactatg gggttattag gattggttgc gttgattttt cctttattca
      601 tactgaaaga aatggagaaa aaagaaaaaa tcatctatgt tggcgtagtt tctacatggg
      661 tatgcgtaac taatttattt ccatggtttt tgtttcaaaa aacaccgata agcttgctcc
      721 agtttccttg gcgtatctta ggtatccaaa tctttttttc ttcaatgatc ataacgcttg
      781 tttttatgaa aaagactatg aataaaaaat attcatggtt aatcattggc gctactgtgg
      841 tgtttctact cgtcacgtct atagcagcaa aagaaaatta cgcaaaagtt atccaatcaa
      901 aacacggtca tattgttatc aatgaagaaa cactcccacg ttatacaaca tcagatatgg
      961 gaggattata cgattacgcc ccaacagaag cgataaaaga aaggcctcac ttagaaaaac
     1021 atgaagtaaa agtaggagaa tcgtgggaaa aagggaagta taaagctgca gataataaca
     1081 ttacttacac agtggcaagc aacaaaaagc aaaaagtgac agtaccagta tatgcttatt
     1141 taggtacaca ggtaaaagta aacggtaaag tcgtaaatga ctcttataaa aaacatggct
     1201 tagtgaatgt ggaatggttg aaacatatgg ttcaaataaa aaaccagtct ttgtcgaaga
     1261 attagatgca ctagctgttc aaaaacaagc aggcttagcc aatgcaccag tgatgattta
     1321 tagcgaagat actacacata tcgtgacaga agaaggaatt gcttatttgt acaaagcaaa
     1381 aacaaaagaa gaacgacaag cagcaattgc agcaattggc ggtgttacac ctcttggaat
     1441 gactagcacg aaagaatcac tagatagctt gcgtgcagcc ggcatcgtga aattaccaga
     1501 agatctaggc atcaaacgta gtgaagcgaa acgttcatta ttagctgcac aaaccatcga
     1561 tgatttgatc gaatggtcag atggcctgta tgaaccacca atgaaattca gaacttggtc
     1621 ataaaattgg cgagaaaaac cttttgtcgc catttaccca gatggaagaa gaaagacgaa
     1681 taagcagaac gtgtatgctc tgttttacaa aaaggggaga gatccatgga acaattagct
     1741 tataaatatc caacgaaaca gccgatcaat aagaaaatcc atgtaggagt ggtcggttca
     1801 ggagatttgg aaatcttgat gtttccaaca gaaaaaacag aatctactgt aaaaatttgt
     1861 acaggaagcg atggcttcgg cgaagtatgg aacaatgtat taacgagatt ttttgatcgt
     1921 tatccaattt cagcagatat cgtgatcaat gacttcggtg ctacaccagg agttgtgtac
     1981 ctacgattga cgcaagcaat ggaggaactt tcagatgaaa aatagttttg tagaattaaa
     2041 cgccagagaa cgagtgaacg ctctacttga caatggacgt gaattgattg atccattcga
     2101 tcaaatcatc gcgccaagtt taccagcaca aggaatcgtc cctgaaagtg atgacgggat
     2161 cgttgtcagc cgtggcacga tcaatggcaa acaagcagta gtaatcgcta tggaaggaaa
     2221 attccaaggt ggcggtatcg gtgaagtttc cggtgcaaaa atcgttgctg cacttgaaca
     2281 tgtgttagaa gaaaataaaa aaggaaatga gatctttcca gtcattgtac tagatacagg
     2341 tggtgttcgt ttgcaagaag caaactacgg actgttatct atttcagaaa tcggaaatgt
     2401 aatcattgct ctaaaacaat atgtaccagt catcggtttg attccaggac gtgtaggatc
     2461 atttggcggt atgtcgatta cttcagcatt aatgagttat ttaatcgcaa caaaaaaagc
     2521 gagagtcggt ttgaatggac cagaagttat tgaacaagaa gcaggcgttc gagaatttga
     2581 ttctagtgac aaagacttga tttggaatac attaggatct cgtcaacggg tagaatcaaa
     2641 aatcattgat gaattagtga ctgatagtgt agaagcaatc aaagctgctg tcatcgcagc
     2701 aatggacgaa cgaaaagaca cgcatcgcag tgaaagctca gcattttatt tatcattact
     2761 tgattcttta gatctttcaa aaccaatggc cattgacgat tacaatgaag caattgctaa
     2821 ccatcaagca aaatcaattg acttaccaga agtagcagaa ggaaatgaac cagctgcagc
     2881 aagtgttggt tacgattggt ttgcggcact aactggcatg aaaaatccaa cttcttctat
     2941 tagtactgtg tattatggta aatcaagtaa attcatagaa gaagccatcg tgacatcaat
     3001 cgtacctgat cctaaaaacc cgatgtaccg tgtacgaaac ggtgaagttg gtttagttga
     3061 aggtttccgc atgggacaaa ttttggatca cgtttacgaa gaagataaag acaaaaaagt
     3121 gaaacggcca atcatcgttg tgatcgatgt accaagccaa gcatacggct ataacgaaga
     3181 attgatcggt atccatgtgg ctttggctaa tagtgctgct gcttatgcaa aactccgtca
     3241 agctggacat ccagttattg gattgattgc aggaaatgct atttcaggtg catttttagc
     3301 acatggatta caatcaagcc gcttgattgc tttaaacaat aaagaaatta ccgtccaagc
     3361 gatgtctaaa gaaagtgctg cacgtgtgac aaaacgttcg gttgctgaaa tcgaagcagc
     3421 tgctgaaaaa gttccagcta tcgcttacga tatcaataat tatacaaaac taggagcgat
     3481 ctatcatttc ctagaagata tcaccgacca agctgcttca gaacaaaatg tcagcaaagt
     3541 catcgaagca atcaatgaag ccattcaaga tgtccgcaat acaaatgaca cgatgctgac
     3601 aaatcgatac acaaacgaaa cagccaaaac attcggacgt gtcgaaacaa ataaagtccg
     3661 tgccaaaatg gacgaagaat gggatgcata aaaaatgatt caagcacatg atattgtcct
     3721 ttttgataaa catgaaatag aagggttcac cttgcctgaa tgggtcgttc taactgaaaa
     3781 gatgttcgga acagttcgtc gtgcgtcgac ggatcacgca gaaatactac cgattggttt
     3841 acgtggacct gcccgcaatc agagatggtc aacagaaatc tcaaaacatg ccgtcaaaca
     3901 agtcattcat ccgtgggaca tcatcgaaca agaatcgtat agacaagaga aaatcatgga
     3961 ttatcctgtt tatcagcaat ttcatgctgc tagaaacata ttatcaggat gtaaatgggg
     4021 aatcggcggc agcttaggat ttgaattatc gactgggatt ccggcagtaa aagaaaccag
     4081 tgattttgat ttgctgctgt acgcaaattc accgattgaa ttgccaattc aggtaattca
     4141 atcgcatcct gctttttttg agcaattcga tacccaagta gtgactcggc aaggcggttt
     4201 ttcactaaaa gagtatttga gagagccaga gaaaaaaatt ctattgaaaa cagtcgaagg
     4261 acctaaatta accaaagaac tttggtaata aagaaaaaga gagagaaaga gatgtttgaa
     4321 aacaaaaaaa tcaaaatcaa cgtaagtgac ttccagaaag tatcagctgt aacggctgtt
     4381 atgatgcaaa cgatattgag ctttgctttg gcaaacagcc acagcgcaac aaatacagct
     4441 tttcttggta ccttttacgt catcgttaaa tacacagcac caatgtttat ttttgcaatt
     4501 gtttataata tggtaaaaac aagtcagcat ttgtcttatt gggaattttt gaaagaaaag
     4561 ttttttgaac tagtcgttcc gtatcttcta tggacgacgg cttatctttg gctatttcca
     4621 gcagtccaac aacaaactcc gtatacggat actgcaagct ttttactgaa atatgtcaca
     4681 ggtgatggag cgcctcattt atggtatacc gttatgatgc tgcagatcca attgttcatg
     4741 ccgttttttg tttggctcgg ttacaaagta tttgctaata aaaaatatgt atggccagta
     4801 ttgattggtt caacgatttt atatgtcgca tggtatctgt tctatcaaac acaagtattg
     4861 actgggcctt atcatgacag ctggtactta ttagatcggt ttgttgctag tttcatgatt
     4921 tatggggtag ccgcatttgt ttatcacgaa aaagtctatc aatatttgga cagagtacgt
     4981 tatcttttct tacctgttgc cttagttatc gcgttctttt ctgtccgttc attgctcgca
     5041 catccaggag acttgagctt tgcgaatgca ccttacttga atacgatcca aagtttatac
     5101 agtttagtca tcatctttgc tgtcttcata ggcgcttcca aaatgattgt aaacgattcg
     5161 ccaaaattgc cattgttcaa atggttgtct gtttatgctt atcgtacgta tttagccaat
     5221 gtatttgttt tccaagtact gttattattg tttaaagata gttggctgca gttaccttca
     5281 ggagtcatga ttcttgtagc ttatctaatg acggcaagtt gtgcatttgc tttaagctgg
     5341 ttacttcata tcatttgggt agctatcaaa aaaggtttca gcaagtaata gtaggaagaa
     5401 ggagtgagtg tcatgataaa tgacgaacga aaaaaagcag tgactcagca actcgctgcg
     5461 ttggtagagg aagcattgat cgcagaagta acattaagtc caaaacctgg attagtggat
     5521 gcactggatg cgggtgcaca ttccgatatg gactatgctt tatttcttaa gtctgctaag
     5581 acgctaactc ctttttttga agaaatggct caaatagcat ggcatcatgc tattgatcag
     5641 gagttaagag aaagaattgc tgagattggg cgaaaagcgg aaaaggcaat gctgattgct
     5701 acaaatggca tcaataccca taaaggagcg atttgggcaa tggggctatt gatcagtgtc
     5761 attgcacaaa aaatcagtat ggaacagcct ttttctttat cgaaaatact tgatgatgtc
     5821 agcaagcttg ctgcttttcc tgatacaaaa tatcgtcata aaaaaaccac tcatggtcat
     5881 agggtgaagc aaaaatatgg cgtaaatggt gcatatgaag aagcgatatt agggtatccg
     5941 catatcaggc ttgcaattca agcagctaag gagctagata atgattcata tgtacagaaa
     6001 caaatgcata tgttgttagt tttgatgagt tcgctagatg acacatgcgt attgcatcgc
     6061 agtgatcaga aaacgttagt ggaaatgcaa aaacttgctc atgctgctag tcaaagtgct
     6121 ttaccaaatc gtccatttac agcactgttg cacttttgtc aggaaaacca aatctctcct
     6181 ggaggaagcg cagatctctt gtctgcaagc ttgttcatgc tgaagataga ggaattatgg
     6241 actgccgaca catacatttg tccgatgtta ctcaatgact aaataagaca aaattgctaa
     6301 aaaaacagtt tatgcatctg gttattgact cttcctttta gcaaggctat cataaaaata
     6361 gttttttgta ataaaagata acgaggaaga caatgaaaaa aatcaattat ttagcaatca
     6421 ttttgttagt gggcctatgt ttgtttgctc tgccgaccca aggattcgct gtcgcgggtg
     6481 gtcatggtgg aggtacatct agcggtcgaa gttcgggcag aggttcattt ggtggtcgtt
     6541 catatggggg cagttatttt ggcagtcgtt cttacggcag agattcttat gggtattatg
     6601 gtccaggtac aggtccttct agttatgagc aaacgggatt tcttgcatta gctgttttgt
     6661 cagtaggagg ggtttctgct ttttctatcg tcaagaataa aagacaaaag aaaaggctga
     6721 aagcggagat attgtcccgc atgtcaggaa cggctaaaga aaagaaagca cagtttctgg
     6781 aaatccaaaa gatattcatg aagatccaat ccgcttggga agaagaaact cctgaaaaag
     6841 ccagaagctg ttatacagac aagctgtttg ccgaacatca gaaagttctg caaaaaaaca
     6901 aagcagacgg tgtaagaaat catactaaaa aaataactat tcaggaagta ggaaattatc
     6961 gacagatcca cgaaaatagt tttgccatcc gaattgattt cagatgctgt gattattcag
     7021 tcgatcgaaa agatgggcga ttgatctcag gaagtaagac acaacgacag tatttttctc
     7081 agatatggta ttttgatttt tctgagaaag aaaaaagatg gcaagccgat tttattcagc
     7141 cgattatgct ggattgacag gaaatattta tgaacggagt tgtgacaaaa gccttgtcgc
     7201 agctcctttt tctgaataaa cggtgtgcca gagatagcct atcggcaata aggcacgatt
     7261 ttgtaaaata tgggaagcga tttttcaaaa tcgcttctta tcgctcaagg ctgaacatct
     7321 ctgtcacaac ctctccacta aatggtgttt aaaagcttgc atctgcgata ataagaacaa
     7381 ccaaacgaaa aataggaagc actattttag tttagttgtt cttattactt gatgcaggac
     7441 agctttttca caacctcttt tttgtataga tgggcaaaat ttttgtaata ataggcgtat
     7501 gtctttttcg ttgaggagat gaaaaagcat atggcttatt ttttgtttgc tttgcttact
     7561 ttctttccac ttcaagtggc agaaatatct actttttatt atgtaagcgg aaccatatac
     7621 ttgaaatcag aaggagatca tttctatgaa aacaagaaca attgaaatac ttcacagaat
     7681 catgacagcc caatatccag taaaagcaaa acatctagca agtgagttca atgtcagtca
     7741 gcggacgatc cgtcaggaag ttcttgaagc gaatatctgg ttgagcagta aaaagcttcc
     7801 ggagatccgt acaattcgca acaaaggttt tttgttgaag ctggaaaagg aaaagcaagc
     7861 tcgattagaa actgaaataa aagatgtgaa agacggcttt ttgaatcggc gcgaacgtat
     7921 ttttgatcta gttttagccg ttgcttacag ccgaatcccg atccatctaa acaaaaaaga
     7981 agaagaattt caaatctcta aaagtacatt agatgaagat atgagacggt tacgagcaac
     8041 actggtcagt tatgggatag agatcgtcag ttatggaaag caaggactgg tttataaagg
     8101 ttcagaacga tctatccgta cgatgatcta tgattttatc aatcagaatc tgggaatgac
     8161 cgacttcttt atgaatcaag atgcacaatt aacaccggct caaaaaatct ttctccatta
     8221 ttttcctttg gtggaaatca aaaaaataca ggagatctat tccaataagt tgttgaaaca
     8281 agaagatgat atttataaaa aacaaatcct gctttttaca ttgatctgga tccagcgaat
     8341 caggaaacat gaatttatct ctgccattaa ttggaaaaat acagaaataa aagacagtag
     8401 cttttctgca tttattgacc acatgatcga ggaatttcaa ttggacaatg tcccgcaagt
     8461 agaaagaaat tatcttcgtt tcactattga gacgttcaat gctagagata tctccaattc
     8521 aatcgagtgg gtacaagctc aattactaac gatccaattg atccaatttg tagagaacga
     8581 gacacacgta cctttccatc taaaggaaga agctttattc gaaaatctct ataagcacat
     8641 ggctgcactg attgtaagaa tcaagaatca tatccaagtc atgaatccat tgaaagaaaa
     8701 catccgaaca aattatttcc caatctaccg cgcagtcact cattttgttc ctacgatcga
     8761 agaagtaatt ggtggcaaga tactggagga cgagatagct tttcttgtca tccacttttc
     8821 gacgatcgcc agtgtaatca agcgagactt gaattatgtt tataaatcag tcgttgtctg
     8881 taaccatggg atggctactg gaaatctgtt agcagagaat ctgaaagaaa agtttcctca
     8941 gatcgagatt actgcagttt taagttcaaa agaaatcgaa ttagtagata aattggatgt
     9001 caatctggtt ttcagcacct ttcaattgaa ttatcgaaat aagccgcttt tagttgtcga
     9061 tccgatttta actgagacga atatcttggt tatagaagac tttttgaaaa gaaatgaaga
     9121 actacagcgg ttagagccta acagtcatga atcgacggag ttattcactc aaataataga
     9181 tgtcatgaaa gaaagtggtg gccgagtaga tcgaatcatc tatggcaagt tagaaaaatt
     9241 attcgaaaca aacaatttag aaatcaacaa aagggagatc cagcctatgt taaaagatat
     9301 tttgacggac agcaatatca tgatcaaaga aaaagccact acttgggagg aatcgattga
     9361 actagctgct agaccattgg ttaaagaaaa cattgtcgaa gagcgctatg tcgaagcgat
     9421 gattcgtgca gtgaatgatt acggaccgta tatcgtgatt ggcaaacaca tggcactagc
     9481 ccatgcccga ccagaagatg gggtcaataa attaggagtc agcgtagcta cgattgagca
     9541 accaatcgat ttcggtaatc ctgaaatgga tccagtaaag attatttttt gtttggcagc
     9601 agtagactcc tactcgcatc tgaacatcat gaaagaacta attgaattga tcaacaacga
     9661 agagaagttg gatcgattga ttgaatgcac aaatatcgaa gatttcaaac aactgctttt
     9721 ttcagtagtt gagaaataat aaatgatacg ggggagaaca aagatgaaca aattgaacat
     9781 tttattcgta tgcggtgcag gtttaggaag tagttttgca gcacagatgt ctgctgagga
     9841 tgttttgaac aaattaggag tagaagccaa actggaccat tgtgatattt cttccgcagt
     9901 ttccatgaaa ccagatgtga tcatcacagc agaaaatttt cgttcgcagt tcgaaaagtt
     9961 cgcagttgat cctaaaacgg cgatgatcta tttgaaaaat attgtttcaa aaaatgaaat
    10021 tgaagaaaaa atcaccccag ttctgcaaga taagggagtt atctaaatac aaaaagaaaa
    10081 gagggtaaaa a
//
LOCUS       DAJSVF010000094        10004 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSVF010000094 DAJSVF010000000
VERSION     DAJSVF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.097"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            39..710
                     /locus_tag="ON550_002360"
     CDS             39..710
                     /locus_tag="ON550_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3955399.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            769..1209
                     /locus_tag="ON550_002361"
     CDS             769..1209
                     /locus_tag="ON550_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955400.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1221..1889
                     /locus_tag="ON550_002362"
     CDS             1221..1889
                     /locus_tag="ON550_002362"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3955401.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1953..2576
                     /locus_tag="ON550_002363"
     CDS             1953..2576
                     /locus_tag="ON550_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR3955402.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2632..3018
                     /locus_tag="ON550_002364"
     CDS             2632..3018
                     /locus_tag="ON550_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR3955403.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3231..4622
                     /locus_tag="ON550_002365"
     CDS             3231..4622
                     /locus_tag="ON550_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR3955404.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5233..5601
                     /locus_tag="ON550_002366"
     CDS             5233..5601
                     /locus_tag="ON550_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR3955405.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5781..6722
                     /locus_tag="ON550_002367"
     CDS             5781..6722
                     /locus_tag="ON550_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR3955406.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6791..7522
                     /locus_tag="ON550_002368"
     CDS             6791..7522
                     /locus_tag="ON550_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR3955407.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7666..9846
                     /locus_tag="ON550_002369"
     CDS             7666..9846
                     /locus_tag="ON550_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR3955408.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9866..>10004
                     /locus_tag="ON550_002370"
     CDS             9866..>10004
                     /locus_tag="ON550_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR3955409.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HL"
BASE COUNT         3335 a         1710 c         2169 g         2790 t
ORIGIN      
        1 tatgctattt taaaaatgat gaaaaaaggg ggagaaaaat gaatttttta gttgttttaa
       61 aagatactct gattgagcga tctacatttc tatatgttca attgcaggaa aataaaacat
      121 tgctgcattt ttcaccagag ttccatctag aggggctaac attaggagaa atctatcaag
      181 cagaagggct gtcggctgtt ttgcactttt ttaaggtaga attggaatta ccagtaaaag
      241 attatatcga gctttctctt gaatcttggg atcgagcagt ggttgaactg tttccagaag
      301 gtttaatgat cgatacaaac gatgggaaga tccatctgac tgcagcagaa ctgcaaaaac
      361 aaatgcgtta tcaaatagat gacgaaaatt ctttttctat ttttagacgt cagcaaaaga
      421 ttctaaaaag ctttttaaaa gtgatcagta aaaaacggaa tctgatcaaa acgacacagc
      481 tgttgaaaaa atatccaggt ctgcttcaca cgtctattca atttcctcaa ttgattacga
      541 tgatccatcg ttttatacgt atgcaacagc taccatctaa gaagctgttt cttccattga
      601 cggacacatt tcgagtagtc aacagagaag ataaaaagaa agtgattgtg attgacttta
      661 ccagaaatcg gaatgtcctt caccaagttg caatggaaaa ggccaattaa ggctttttct
      721 ttttggattt ttttgtcata ataaatatta gatgatggag gagataatat gatgcaaaca
      781 tggttagaaa ggctaatacg atttcagcag aactatcaca aacttatgca gggaagatat
      841 gcgaaatttg atcagttgaa tcgcggtcta ttgatcgctg cgcttgtctt tagtttgcta
      901 agtcgttggt tgccctatag aatcggacaa ttgttgttca tcgtcttttt cggatggatg
      961 atatttcgtt tcctatcaaa aaaaatctac cccagactaa atgagaatca acgttatttg
     1021 aaatatcttg atagagtcaa agaaaaatat catcagataa aaaacaaaaa gcaagtcaaa
     1081 atggatcgaa aaagttatac tttttttgag tgcccgaatt gtcatcaaaa gcagcgtgca
     1141 cctaaaggaa aaggacgaat acgtgtgaca tgcaaaacat gtggtataaa attcgaaacg
     1201 aatgtttaag ggagaaaaga atgaaattag tattattacg tcatggcgaa agcgaagcaa
     1261 attttgaaaa ttactggaca ggatggctag atgtcgcctt aacagaaaaa ggaaaagaac
     1321 aagcagcagt tgccggtcaa aaaatgcttg aagccggact tcaattcgat gccgtttata
     1381 cctctgtttt gaaacgtgcg attcttaccg gacaagtcgc actagaagaa atgggtcagt
     1441 tatggcttcc tataataaaa acatggcgtc taaatgaacg gcattatggg gatcttgtgg
     1501 gtaaaaacaa agataagatg aaagaaatat acggaaaaga tcaagtaaaa acatggcgca
     1561 gaggatatac ggaagttccg ccgttaaccg cagaaaatag atttgaccgc agatatgatc
     1621 acttggatcc gcatttgatc ccatacggag aaagtctgga aatgacagtg aatcggatca
     1681 ttcccttatg gcaagatcat ctagcaccaa aactaaaaga tcggcaagat ttactagttg
     1741 tgggtcatgg taatagcatc cgagcactga caaaatattt agaagatgtt cctgaagatc
     1801 aaatggatac gatcgatatc ccaaatgcac aaccaatcca gtatacatta gcagatgatt
     1861 tgacaattct ggacaaaaaa atactttaaa gaacaccttt atctaaaagt gagagataga
     1921 aaaaagaagc aaaggaatgg aacaaaagga acatgaaaaa ttatatatgg gactttgatg
     1981 gtacattgtt tgatacgtat cccgcaatgg tagatggtgc atggcaagca ctaaaagatt
     2041 ttggtatcag tatggacaaa aaagaaatct acttcaaaat gaaaaagtat tctactagtt
     2101 atttgatcaa tgaatcaaac ttgaatgcca gagagtttaa tgaattattt caccgatatg
     2161 aaaaagaaag tacggaagtt tcccgtccat ttccagaaac gaaacaagta ctggaaatgc
     2221 taaaagacaa cggcggtcgt catttcatcc tgacccaccg tttgacagaa tcaacatggg
     2281 gcctgttaaa agaacatcga ttggcacatc tgattgagga agtagtaggg atcgatcagg
     2341 actttcctcg taaacctgat ccagcatctt taaattattt gatcgatact tttcatttag
     2401 aaagaacaga cacgatgatg atcggcgatc gtcgtttaga tatcgaagca gggaaaaatg
     2461 caggagtcgc tacttgtctt tacgacatcg atcattttct gggtgagatc ccggcagatt
     2521 atgtggtagg aaatctgaat gaaatcctta ccttgcagag catcattaaa aagtagtatc
     2581 ccctttcaaa ggagatatgt tataattttc ataaaggagt gataaaatta tatggaaatc
     2641 agaagacaaa aagaagttgt agaagcattt cattatgata tgagattgcc gaaccaagaa
     2701 gtagaaacag atttgaaagt aggcttctcg cctttaaaag aaacgatcga aaactatccg
     2761 aaagaacata gcatcgtagg agcacgctta gagttccgtt tagtgtttga tacatatgtt
     2821 ttatctggaa gtgtgagtca gatcaatcat atcctcaatc ggaaaattga aggtcaaaat
     2881 gatgtcacac aagaagaagt ggacgagcta gtagcaccgt tattcgatat cgtacgccga
     2941 atgtcttatg aggtgacaga gatcgcctta gatcagcctg gggtaaaatt gaattttcaa
     3001 tcagaaaaac aagaatagac agtaaaaaca tacctgcgat agattaaagc tatcacaggt
     3061 atgttttttt attactaatt gtaagcaaaa agaatgtcat ttcgataaaa atcgtctaaa
     3121 atataaaagt cactgaaaaa aattttttca ggaggatggt tgaagaatca ttctcgggaa
     3181 cctttacttc cttcgatgga cttgtcatta ataatatgag gaggagaaaa atgacaacac
     3241 tcatcacagt tttagtggtt ctagtttttg cggcattatt atatgtgttc tatcgtatgc
     3301 aaaagcgcca tacaaaattt tcaacacgcg tctttacagc attaggagtt ggtatcgtct
     3361 ttggcggtct gcttcaattg atctttggtg tagacgacaa agtcgttact caatcaatgg
     3421 aatggatcgg gattgtaggt caaggatatg tatcattctt gcaaatgcta gttatgcctt
     3481 tagtgtttat ttcgatcatt ggtgcattca caaaaatgaa agtttctgaa aagctaggga
     3541 aaattagtgc gactgtattg acttctttac ttggaacaac agctattgca gcattgatcg
     3601 gaattggttc aacaatgctg ttcggactcc aaggagctaa attcacacaa ggagctgcag
     3661 aaacagcacg aatctctgaa ctggcaacac gtcaggaaac agttgaaaac ttatcgattc
     3721 cacagcagat tttatccttt atccctaaaa acgtttttgc tgatttagca ggtagtcgtg
     3781 caacaagtac gattggagtc gttattttcg cggcatttgt cggaatcgct tatctaggcg
     3841 tgaaaagaaa agcgccaaaa gaagcagcat tcttttcaga tttagtcgac agtctgtata
     3901 aaatcattat gcggatcgtg acgcttgttt tgcgtttgac accttatgga gtatttgctt
     3961 tgatgacaaa cgttttggca acaagtgatt ttgcagctat cgtcaattta ggtaaatttg
     4021 tcgttgcttc ttacgtagca ttgcttgctg ttttgattgt ccactcactg attttgattg
     4081 gtgcaaaagt caatccaatc aactatttca aaaaagcctt tccggtattg agctttgcgt
     4141 ttacttcacg ttcaagtgct ggagctttgc cattgaatat cgagactcag acaaaagctt
     4201 taggagtaga tgatgcaaca gctaatttct cagcaagttt tggtttgtct attggacaaa
     4261 acggctgtgc tggcgtttat ccagctatgt tggcaacgat cgtcgcacca gcagctggag
     4321 tgaatgtctt tagtttggaa tttattttga tgcttgtggc aattgtaaca atcagttctt
     4381 ttggtgtagc tggagtcggt gggggcgcaa catttgcttc actgatcgtt ttaggggcaa
     4441 tgaacttgcc agtagcaatc gttgggttag ttatctctgt tgaaccatta atcgatatgg
     4501 cacgtacagc agtaaacgtc agcggtagca tggtagctgg tatcgtgaca agtgcacgca
     4561 tcaaagattt agatcgcgat gtcatcaatg atgattctaa agtgatcgat gtgaatgcat
     4621 agtcaaaaag aggttgtgat agaagtggtc agctttaaga aataagaaga agtttcctaa
     4681 aaatagtttc tcaaattttt gtgaaatttc ggcttatttc cgagaagcta cttctgaaac
     4741 accgtgaata agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
     4801 aaaatagttc ctcatatttt acgtttggtt gagcttatta ccgcagatgc aagcttttga
     4861 acaccgttta agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt
     4921 tcaaaaaata gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg
     4981 agaacaccgt ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat
     5041 tccatataaa tacacattat ttattccgaa aagtgaaagc gtaaataaca aaaacaacca
     5101 acttatttat tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt
     5161 agattttccc attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt
     5221 aggaggaaac agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa
     5281 aaaaatgcaa cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat
     5341 gggacgctta taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat
     5401 cgagcatttg ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt
     5461 tacagtaaag atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt
     5521 gatatggtac agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac
     5581 tttgcggtta caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa
     5641 gcgaagtctt tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat
     5701 tatacacaag caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa
     5761 aagaaggaag gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa
     5821 tcgaagaacc agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta
     5881 aaggtcgtat tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa
     5941 acacgactgc ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca
     6001 agatcgatcc aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc
     6061 ttgtaagttt aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt
     6121 caccgcaaga atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga
     6181 atgattatga atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt
     6241 ttcgagagct tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag
     6301 tgacctactg tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag
     6361 gattcaaaga tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag
     6421 tccaaggaga attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata
     6481 tcaaccaagt agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac
     6541 gatatatcaa ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg
     6601 aagaaaaaca tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg
     6661 ttcaagacca tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat
     6721 agaaaacaat agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat
     6781 ggtgatccac atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct
     6841 ttttgaaaaa cttattcact attatgatcc aaagatccat cagcttgttt tattagggga
     6901 tctcaatgat cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga
     6961 agaaacagga gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca
     7021 atcaccggag gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag
     7081 cctgattcac ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca
     7141 atcgagatat tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga
     7201 aaattatctt tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag
     7261 tctaagagac tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa
     7321 gacaattgta tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga
     7381 cctctggatt caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt
     7441 gcatggggtt gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga
     7501 aggaccttgg catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg
     7561 ctctttttat ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt
     7621 atgatagaat aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg
     7681 aaccagacga tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg
     7741 actactgtac ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga
     7801 aaagaaatga ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc
     7861 tatcttgaaa acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg
     7921 aaactgactc ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa
     7981 gatttgtatc gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa
     8041 ggacttgaac cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa
     8101 gcagaaaaat atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt
     8161 gctcatgaga ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg
     8221 tcttatacgt ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa
     8281 aaaggcgtat atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat
     8341 cgaatattag ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta
     8401 gatgaagcag caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct
     8461 tcttcttcat tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca
     8521 atcgagcgag aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt
     8581 ttcggcgaaa acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg
     8641 ggatttgatc cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa
     8701 gttttagcaa tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct
     8761 gctggaccag cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga
     8821 aatggtacag ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac
     8881 cataaaacct attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa
     8941 attgcacgaa aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc
     9001 cgtcgcctgc aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta
     9061 ggccagtacc aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg
     9121 gaaacagcgg ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag
     9181 catatatcag gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac
     9241 ggagaattca ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat
     9301 gaacaagcaa tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt
     9361 atccatcccg aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa
     9421 aaagagcttg gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg
     9481 gctgaaagcc tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa
     9541 cctggacgag atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt
     9601 atggaagact tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc
     9661 ggcgcatttg ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc
     9721 aaaaaattcg tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg
     9781 atagaacaag tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa
     9841 gagtaacaat gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga
     9901 gatatcatta aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt
     9961 acgtacaact ggcgggcgct atcatttaaa cgatcatcat ttag
//
LOCUS       DAJSVF010000095         9974 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSVF010000095 DAJSVF010000000
VERSION     DAJSVF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.098"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..807)
                     /locus_tag="ON550_002371"
     CDS             complement(<1..807)
                     /locus_tag="ON550_002371"
                     /inference="COORDINATES: protein motif:HMM:NF013429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3955410.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTYSNCQAVKQLAQKHALDIIAFTPESIIYPYNIAAPETMQWEKSKAYFRNAILA
                     TAELGCSLMTINSGYGLMEEPIEESWKRSREMLRYLAEIAETEGVTIALETLRPEESK
                     IVTKLQEAQRMVHDVAHPSLKLMVDTAAMGVAGEGLQDWFKAFGDDIVHLHFVDGTPE
                     GHLAWGDGNFNLHEFLTILEKYDYQGFLGQEITDSRYLKRPYLADKQVFDK"
     gene            complement(840..2240)
                     /gene="yfcC"
                     /locus_tag="ON550_002372"
     CDS             complement(840..2240)
                     /gene="yfcC"
                     /locus_tag="ON550_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3955411.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFVGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            complement(2288..3787)
                     /locus_tag="ON550_002373"
     CDS             complement(2288..3787)
                     /locus_tag="ON550_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR3955412.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNVSTEDFFNYCRLEESG
                     VTVTSGTDLPLFITDIPESIIRAVFRRFPEGEEVWEKETSFTTTELLKSFTSNSFKVN
                     GLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMAAEVIATYLDGDLVYKK"
     gene            complement(3823..4533)
                     /locus_tag="ON550_002374"
     CDS             complement(3823..4533)
                     /locus_tag="ON550_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3955413.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKQMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(4601..5416)
                     /locus_tag="ON550_002375"
     CDS             complement(4601..5416)
                     /locus_tag="ON550_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR3955414.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKMGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(5427..6395)
                     /locus_tag="ON550_002376"
     CDS             complement(5427..6395)
                     /locus_tag="ON550_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3955415.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            complement(7129..8329)
                     /locus_tag="ON550_002377"
                     /pseudo
     CDS             complement(7129..8329)
                     /locus_tag="ON550_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
     gene            8350..9123
                     /locus_tag="ON550_002378"
                     /pseudo
     CDS             8350..9123
                     /locus_tag="ON550_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9302..>9974
                     /locus_tag="ON550_002379"
     CDS             9302..>9974
                     /locus_tag="ON550_002379"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3955416.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMN"
BASE COUNT         3084 a         2070 c         1613 g         3207 t
ORIGIN      
        1 tttatcaaat acttgtttgt ctgcaagata aggccgtttt agatagcggc tatctgtgat
       61 ttcttgtccc agaaatccct gataatcata tttttctaaa atagtaagaa actcatgtaa
      121 attaaaattt ccgtctcccc aggcaagatg tccttctggt gttccatcaa caaaatgaag
      181 gtggacaata tcatccccga aggctttaaa ccaatcttga agaccttctc cagcgacccc
      241 cattgctgcg gtatcaacca tcagcttcaa agatgggtgt gccacatcat gaaccattcg
      301 ctgagcctcc tgtaatttgg ttacaatttt cgactcttcg ggacgcaacg tttccaatgc
      361 aatcgtgact ccctcagttt ctgctatttc agcgaggtat cgtagcatct ctcttgagcg
      421 cttccagctt tcttcgatag gctcttccat caaaccatat ccggagttga ttgtcatcag
      481 tgagcagcct aactcagccg tagctaatat tgcatttcga aaataggcct tgcttttttc
      541 ccactgcatc gtctctggcg cagcaatgtt ataagggtag ataattgatt ctggagtaaa
      601 agcaatgata tcaagagcat gtttctgtgc tagttgctta acggcttggc aattagaata
      661 agtaaaagca tctatgaaaa aatgcggagc acctccccaa agttcaacag aagaaatacc
      721 aattttctgt tgataagcga aaaattcttt gagagaataa aataaatagt ggatattcat
      781 tcctgctaat tgatttattt taatcataac tattgcctcc tattaatgga tgattaagac
      841 taaaagagtg tagcaatgta taacaaaaca gcagaaacaa gggttaagat ggcaaatatt
      901 ttgaagccaa ccttaaacca tttagagaaa ttaatctctg cgatggctaa tcctgccagt
      961 aatacaccac tagttgggga gataatattc attaaaccat ttccaaactg gttacttaga
     1021 acaacaacat cacgacttac attcattaga tcacccaatg gtgccataac cggcatcgtt
     1081 aatgccgcag ctcctgagga ggatggaaca aaaaaggaaa caattacttg agaaatgagt
     1141 agaatcgtag tgaacaggaa ttttggaaga ccacctaaaa ggttagccaa gctgtttaga
     1201 atagtgtcaa taatcattcc accttccaag attaccaaga ctcctctagc tagcccaatg
     1261 attacagcag cataggcgaa atctgaacag ccttcgacaa aagcttcagc aatttccttt
     1321 tgagaaaatc tagcgatgcc cccagcaaaa agacctaatc ctacgaatag tgcagaaatt
     1381 tcaaccatat accatccctt ggaaatgata ccccaaatca tgattcctaa acctacgata
     1441 aaaccggcga taattagttt atcttgtgtt gaaaactctt tttcagtgtc aaaattttga
     1501 aacttatgct taacctttaa atcactctca taaacaatag aggacagtgg attctttttg
     1561 actctgctag cataaagcat ggtgaaaaca attgaaatcc cagtaatgac aatccaggat
     1621 agcatccgaa accaaagcag gggatttccg taaacgccag ctactccctg tgcgattaaa
     1681 acgttgaagg gattaatcgt cgacccaaca tatcccgctt gtgtacttaa aaataaaatc
     1741 aagatcgcag tcatggaatc atagccaaga gaaaacatca gagaaataaa caccgcaaat
     1801 aagggaatga cttcctcgct catgccaaag gttgatcctc ctaagctgaa tagaatcatc
     1861 gacatcggaa taactaagat ccccttgtct cccagaactt ttgctaaggt aaacagccct
     1921 cgatcgatgg ctcccgtttt taggataatc ccgaaggcac caccaacgat gagaacaaaa
     1981 gcaacaacct caacagctga aataatccct tcagctggtg ccattagtat agaggaaaaa
     2041 ccttgtccat aatctgcctt atcgatttgg tgataggttc ccggaacaac cgttgttttt
     2101 tccatacctg cgacgttttc aacaactgtc tcaaactgac cactaggaat tatccaagtt
     2161 aaaattgcag ccaaaataat taagataaaa atcaatgtat aactatgtgg gaaggtgaat
     2221 tttcttttca ttgtttccat tttttaacct ctctttaatt cgtcttataa cgttatatcg
     2281 aaaagcatta tttcttgtaa actaagtcac catcaagata ggtagcgata acttccgcag
     2341 ccatcacttt ttgccagtca tcagtacaga ggtcctcgct gaaaacagct aagctggcgc
     2401 ttttccctac ttttatttct ccgtactccg ataaaccatt tactttgaag ctgttgcttg
     2461 taaaactttt cagcagctcg gttgtcgtga agcttgtttc tttctcccag acttcttctc
     2521 cttcaggaaa acgacggaat actgctctta taatcgattc aggaatatca gtaataaaca
     2581 gcggtaaatc tgtaccgcta gtgactgtta caccactttc ctctaatcga caataattaa
     2641 agaagtcctc tgtggaaaca ttttttataa cagttgacat gtaagcctct tctaacgttt
     2701 gattcaagcc gagaatttgt ggatatacct ctgctactac ttgtccttcg ttcatttttt
     2761 ctgctgtctt ttgagtaacc atttccaaat cactaatcga attaaaaacg ccttcaggta
     2821 accgctttcc aaattcgaca agaattgacg cagctttata acccgcttta tctccttccg
     2881 ttgtcaaaca gcaaggaatc tctacttcat tgaatgcagc aacttctgct ttaattgcct
     2941 catagtcaat ctcgggtgaa gctactcctg actgataaac accgtaaata tctccacttc
     3001 tatccgcaac aacaccatct accatgatct tcactccacc aaagcggacc ttttcaggga
     3061 aaactttttc cttatactca tatagaagtt catgctgtag aggttcttgg actgcctgca
     3121 catataggtt actaagtatt ttcctctgag gtatattaga aagaaattct gcgtcatcaa
     3181 aaacgatttc tttcattgat ataacacctt ttgaaagcag taatgcttca aattcagcgt
     3241 aaacctctgc taaaagcttt ttgtttgcta gcatttcctt aattaataat actcgacttt
     3301 cagcactggt ttctttttct gtaaaaccat aagtctgctt agcaatagca ttcatccaac
     3361 aatggctgcg atttttgtcg atcgctgtga ttggaccaaa gtggtttatt tcattcaata
     3421 gctcttctga tgggtcagtt ttccacaggg cagcatccca gccatgagca taaattggtt
     3481 catctgcttt tttatggttc attacagctt taatacgctc tacagcctcg ttagctgaag
     3541 atgtatcgct taaatctatg ccagcattca tagccaaata accactgaaa aatacatgat
     3601 tatcatgaat tgctggcata acgaattgat catcataagc aattaattcc gtgttttcat
     3661 ttaatagatc actgtaattc cagttgtttt ccaccttttc aatgatccct tcattgatta
     3721 caatataacc tttgaaaggt ggctgttctc catcaaaaat catttttgac cgcaatattt
     3781 gcttcataaa taaaatctac tccttgaaat caaaacagga aatcataagt aaaaacgata
     3841 tacttctccc aggtaatatt gattacctac atgaattggt atgttgtttt gatcgttgat
     3901 taaagtatcc ataaagaaaa agggcgtccc aacagctact tccattattt tggcaatttg
     3961 gtcatcagct gtgacaattt caagtgcagt ttccccagga ttgatggcta aggtaccttg
     4021 gactttaagt acttcataca aagaatcagt cagctcttcc tccattaaga aactatatct
     4081 ttcataagaa aaataattgt tttctaacat aatggggatg ttatccgcca tttgctttct
     4141 ctggatatat aaaacatcat caccttgagc gatatcgagt ttttttgcca tcccttcagt
     4201 tgctggaatg atcttgcgtt ccaaaacaat gctagtcggt gtaaaaccat tagatttaca
     4261 actctcactg aaacccataa catattcaat tttacgaaaa actttatgcc ggttgacaaa
     4321 agttcctttc ccttgttttt taagtaggta cccttcttct accagttctt ctactgcttt
     4381 tctcactgtg attcggctga cctcataact ttctatcagc tctgattcag cgggaatttt
     4441 cgtattctcg ggatactccc ctgtattgat tttatttttt aatatttctt ttaattgtaa
     4501 gtacaatggt aattgtgcat ttgtatccag cattttttta acctccactt taccgggatt
     4561 tctttcttta ggttatcaaa tgcaggaatt taaaacaagc tcaatcgtaa tgaataccaa
     4621 agccgaacga accgtcaacc agacaagact tcccagcaaa ttcattcccc attttcatcg
     4681 cggtgactat atcgtcccct tcgagaaaat aaagtaggaa agctgttaag aaagaatctc
     4741 ccgctgccat tgtatcaact ggttcttcta gcacggggtt cacttcataa aactttgctc
     4801 catcataacc aatagccgtt ttttctcctc gggttgcaat caatacatca tttcgctcac
     4861 aaaaatgctt catcaaaaac tgctttactt cttcatcgga aaagtcttct atagaaaaga
     4921 agccaaaatc aactttatca atcgcccgct gaacctcttc ttccgtaaag tcgtcagaaa
     4981 aatcataaga aatctttcca gaaacatttt gtaactcttc taatagatga ttgacatggc
     5041 tatataatcc catatgaatc aattcaaact ggtttaaata ttctctgtct tcttcagtaa
     5101 gctgaatcgg gtattcacga gtaacacctc ccgaattgct gccaaggaaa atccggtcgc
     5161 cctcatcaat cttcactctg gcacagccat tctcgccttt ttcaaagcga gagtgactgt
     5221 tgtccagtcc tatttctttt attgctgata aaacatgagc tgcctcctca tctgagccaa
     5281 aaactcccat aaagctactt tcatgcccca gtttttttgc aaaaactgca aaattcaatg
     5341 aatttcctcc aggatacata attcccagat tttcatattt atcgacgacg ttatcaccta
     5401 agcctaatac cttcatttta atcctcctag tactccactt tccccatata gcgacgaaca
     5461 tctaaatcat gattgcgtac atctgctaaa gctgaacgat attctgaaag aatactgtag
     5521 aataaaatgg gattgaagaa ttcagaaact tcatctgata tcatatccaa tcctagttcc
     5581 tttgcatcaa cgatttctac tctttctgca tatttatcaa ggaatcgttt tgcccgttca
     5641 tctaaagcac gagtgcgtcc aatgctcatc aagagaataa aattcgtatc tttgtctgtt
     5701 acttcaaaag gtccatggaa gaattctcca gaatgaacag aagttgaatc aagccattgc
     5761 atttccatca aagagcaaat tgaaaaacca taagcatgtc catatgatgg tccactacct
     5821 aagatataaa acattgtttc atctttgtac ttatcagcaa acactttaat acgatcctgc
     5881 acctgcttaa tgccattttc aattacggta tcgataacct tcatgccttc tctgaattta
     5941 ccgtaatgtt caaatgtttc ggtaacattg accaaagata cagttaaatc aagaatgatg
     6001 gccataggat tattgactat ttttgcatca tctccccatt cgtaaaggaa actatgatca
     6061 gaaaccttta ataaatccgc agttttatta tgagtcagac caacagtcac agctcctcta
     6121 gcttgagcga cttctcctgc ttttaccgtt tctgctgtat ttcctccatg agaacataga
     6181 atgacaacag atttatcatc taattgcttt ggggttacat ggttaaattc gttcgctgta
     6241 tataagcctg taatacactt cgatgcttcg gctttaagaa aataatctga gacatacata
     6301 tctaccagcg aaccgccaca agccaccaaa taaattccag aaatctctcc atcatttttt
     6361 tctacaattg cgttaaccgt ttttgctaca tccatcaaca tttcctcctt aaaattttaa
     6421 aaatgttaat tttatccaaa ataaaagtaa ctgattaaat gtaaagatat tacataacgt
     6481 catataacgt catgtgacat aattgcattg tatccgcttt ctttgtgttt gtcaatagga
     6541 aaaatttatt tctctaaaaa atataaataa tctgcagtga aacttgagta catatactaa
     6601 tttcgaaaaa tgcctatgat ttaaataatt atggtgctgg gatgaaggtt tataagtttg
     6661 tcttgatttg tttagaagaa atcgtttttg cacacagttg aattaggaaa ctactggagt
     6721 ggaaaataaa gtgatttgac gggtagatgg gtgcacttaa atttgaaaat ggcttaaaat
     6781 gctttatttt gaattgcaga atttctttta gtacgcaatt tatggtctta aaactgatta
     6841 aaaagtgata cattgatttt aatacaattt atgatacaaa aaatgacctc ctttcttaaa
     6901 taaaagaggt cattttgttt tgtctcataa tcgattgttt ttgatacagt agttatattc
     6961 agaaaaaata aagactcttc tgagttgata atcctttgga ttatatacgg atacagatgt
     7021 agacataaaa caagctcaat tttcaaaaat ggcattactc acctttttgg tgaattatta
     7081 aactaaaaaa gatgaatttt tttatgtact ataagatgta ggtgtactct agttaaagta
     7141 attctttatg tactctgtaa catcgaatac atcttctaat aaaaatgcac gcaataatct
     7201 gtcaattagc atatttacct tgattggtat taccatttta cctaaatttt tctcgatata
     7261 tccactgaaa tgacctacgt taacacttga ttttaattta tcaaatcgac tatattcatt
     7321 aatgtttacc tcggttaagc gtaaatccaa catctcacgt aataatgcct cattaatatt
     7381 ggttgaatcc actaaatctt ggataaaatc gtcctcttca ctaagctgat attcattgag
     7441 gtaatcagta aattcccatc cttctttgat aactaattcc ccattattta catcatttag
     7501 aatcatctca gcatatggct gtaactccac tggtaattgc gcaaatgtgc gatgtaagtc
     7561 gtttaacaat gattgaatta aattcgaatc acgttcttct tttatattca tgtatttttt
     7621 gaagtttgtg tttaaatact cataattaat acgactacga ctaccttcag gtaattggta
     7681 atcaatgtca tacgctatac tatcatttcc gtctctaggt tctctctatc tcgctgtaac
     7741 gttgtgccaa gatattaaat tcatattctg tgattactac tgttataggt tcataatcct
     7801 ggatttcata agtcgatttt gcccatgtga atccctgtat catcgctgat tcataacaat
     7861 ggattagttg attataaaga tttacaaatt gcttaatttc ccctggaacc tccggtaaat
     7921 gactgaagtt ttctatgcca gaaatattaa atacggactt aatttcttcg aatatatcgt
     7981 tcaacttttc taagttatta tataattttt caacaaataa cccttttgct tgtttgcctg
     8041 agtataactc aatagcctct tcaatgttct gttccatcgt atgtgcttta cgataataac
     8101 gaatggttcc gaacggtttt tcaggaccat taagacgatt tgtgcgtgaa aaagcctgaa
     8161 ttatgttctg atattcaagt gacttatcta agtatagggt gttgacccat tttgaatcgt
     8221 aacccgttaa gagctgatta acaactataa caagatctaa ttgggttgct tcatctactg
     8281 ttttgtaagg attcttatgc gctgtgtttt gcaatagttt tgtacacaag attgcaaaga
     8341 aaagtacaca atgttgccac ttctggttcc atgagtttta tttgtgttga tgggacaacc
     8401 aatcagctat ttacgaccct tgaagatcgc agactttaca aactaactca atacttcctg
     8461 cgctttccta gaaaggcccg tttaaaggtc aagtacctag tgatggacat gaatgccagc
     8521 tattgtcagt tgcttaaaac ggtctttcca aatgcggaga tcgtaactga ccgctttcat
     8581 attgtccaac acatcaatcg cagcttcaat caattacgtg tccagatcat gaatcgtttt
     8641 cgaacctcac actctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc
     8701 ttactcaaag attccacgac atgggaggca ctcaaacggc attatcatcg actgtttaaa
     8761 cgaccaatta gtcaaacaga aatcgttaat gagctgttaa gctacaacga agaattgcga
     8821 actgcgtatc atttttgtca attattacgc tattatttcg ttaaacgaga tacggtaggc
     8881 tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa aaagaaattc
     8941 acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgtc ccactcaaac
     9001 ggcgtcactg aggggctgta taacaagatc aagctgatta aacgaattgc gtttggttat
     9061 cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat ctttgaaaac
     9121 taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta ttggctctat
     9181 aatttctagg actgatggaa attttctatc tgtcctagtt tttttgtaaa aaaacaaaaa
     9241 gacatacaaa atttccggta gaatagagtc tcctacaaca tcattctggg ggaattcttg
     9301 tatgtccatg aatcatttta tcaaacaact ctttactatt aaagactcaa atatccaaat
     9361 tcaatccttt gaagaaactt tttataaaag tagaaaaagt atcgttattg agggaactct
     9421 gatccgtacc tacagacgtt gtccgtgttg taaagattct acaaagcaaa tcgttaaaaa
     9481 tggcaagaag atctctatga ttctcctgaa tcgtagcggt aataaacgga cctatcttcg
     9541 gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa tattttaccg ctcggacgta
     9601 tctcgttacc ccattctgct ttatttctaa gcagattcat tacaagatct tagaagaatt
     9661 gacagagcgt caatctataa aagcaattgg taaacattgc gatgtttctg tgacaactgt
     9721 tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag aatcttgagt atttacctcg
     9781 gtgtctatta ttcgatgagt ttcgttctct ttctacatgg cacggtaaat tttctttttc
     9841 ttgtatggac ggtgagaccg ggaaactgtt tgagatctta cctagccgcc gtaaaaaaga
     9901 tctcgttgct tttttcatgc gtttttctct caaagcgagg ttgggtgtac attacatcgt
     9961 aacggatatg aacg
//
LOCUS       DAJSVF010000096         9931 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSVF010000096 DAJSVF010000000
VERSION     DAJSVF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.099"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            70..456
                     /locus_tag="ON550_002380"
                     /pseudo
     CDS             70..456
                     /locus_tag="ON550_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            470..1159
                     /locus_tag="ON550_002381"
     CDS             470..1159
                     /locus_tag="ON550_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955417.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1284..3635
                     /locus_tag="ON550_002382"
     CDS             1284..3635
                     /locus_tag="ON550_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR3955418.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3715..4110
                     /locus_tag="ON550_002383"
     CDS             3715..4110
                     /locus_tag="ON550_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955419.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4435..5052
                     /locus_tag="ON550_002384"
     CDS             4435..5052
                     /locus_tag="ON550_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955420.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5093..5392
                     /locus_tag="ON550_002385"
     CDS             5093..5392
                     /locus_tag="ON550_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR3955421.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5426..5593
                     /locus_tag="ON550_002386"
     CDS             5426..5593
                     /locus_tag="ON550_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3955422.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5638..6243)
                     /locus_tag="ON550_002387"
     CDS             complement(5638..6243)
                     /locus_tag="ON550_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955423.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6368..9355
                     /locus_tag="ON550_002388"
     CDS             6368..9355
                     /locus_tag="ON550_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR3955424.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9520..9930
                     /locus_tag="ON550_002389"
                     /pseudo
     CDS             9520..9930
                     /locus_tag="ON550_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT         3431 a         1483 c         1901 g         3116 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct
      121 gaacataata ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa
      181 ttgtataaag aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt
      241 agtgactcag ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa
      301 tccgaattag ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc
      361 aacgctattt cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag
      421 cagataaaag aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat
      481 ttttggtgtt tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc
      541 tgagaaagaa gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga
      601 agaagaagat aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat
      661 tgtatctatg gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc
      721 ttcagtttca gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga
      781 aactactcca attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct
      841 tcttgtacta gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg
      901 ttttggaggt atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc
      961 ctacattgac gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc
     1021 tattcaagga atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc
     1081 tggttggaca atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta
     1141 tgttgttcaa aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt
     1201 tttagttgtt gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt
     1261 cggcaagaaa gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa
     1321 acaagaaata cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt
     1381 ggcctacaat ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag
     1441 tagctaattt tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg
     1501 gcaaaaatct acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga
     1561 ttaatccagg tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt
     1621 tgcagactga tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg
     1681 ccaaagcgtt atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta
     1741 gaaaaatgca gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg
     1801 atggtattta tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag
     1861 gatattctcc cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc
     1921 ctgtgtctga aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta
     1981 atggttattt tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag
     2041 tactggcatt ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg
     2101 taataaaagg actattgtct agcgctggag atattagaag atctgcagat ggcgcagata
     2161 cttctacgca attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag
     2221 ttggtagata tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa
     2281 ctacagagga attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag
     2341 atggaggtgc agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg
     2401 ctatagatgc agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg
     2461 atctagacat gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat
     2521 ctgctgtaat gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt
     2581 ctcaaataat caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt
     2641 atagtgggaa tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat
     2701 ttaccatagg aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag
     2761 atagcggatt taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca
     2821 atcagaaggt tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg
     2881 ataatccttg gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat
     2941 gggacatcat ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga
     3001 ataaattaac taatagcgag ggcctgtata attattttat agatcctaat actggttcaa
     3061 taataggttt gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa
     3121 tcaatgatag tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg
     3181 aagttaagaa attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg
     3241 caggtggaac atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata
     3301 ccttagcaaa aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg
     3361 ataagggtgc tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata
     3421 aaatcgcttc agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg
     3481 tagggataat ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta
     3541 cttatcttga ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta
     3601 ttcaatttca agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt
     3661 ccttttcctt tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg
     3721 attatggaag aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt
     3781 ggaattatct ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag
     3841 atagtagatt actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt
     3901 gaaagagtac caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag
     3961 tctccaaatg aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag
     4021 aaagaataca attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa
     4081 ttagaaatat atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga
     4141 agtcaaattc tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat
     4201 aactagttta tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat
     4261 aaatataatc atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc
     4321 ttgattttct ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg
     4381 atttaatctg attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga
     4441 aaattttttt atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc
     4501 gcgaaggaat taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat
     4561 tttaaacgcc ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat
     4621 ctacaatcat tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa
     4681 ttaactaagg aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct
     4741 agttacaagc aactagatgg gttagagacg cttatctctg acttaattct gcaattactc
     4801 agctatatgg ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa
     4861 gctgcgaaac aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt
     4921 cctgcgatct attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa
     4981 ttgggcatga aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac
     5041 cacttttctt gatagatata acagctatta gacgggaagg gagagtttga aaatggactt
     5101 aacgaatcaa agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat
     5161 cggtttggtt cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt
     5221 cctggtttgt ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga
     5281 acaaaccgcg cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga
     5341 attttatgcg ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt
     5401 aaaatttaca aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga
     5461 aataactagt tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc
     5521 aaaaagtgtt tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt
     5581 acatggaaca tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta
     5641 caaatcattc gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc
     5701 aatctctgca actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg
     5761 tttttgcgga tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc
     5821 tttttgagcc aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg
     5881 tgcaatataa cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt
     5941 tccagtatta aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt
     6001 cataaaagca actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt
     6061 aacgatgacg caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc
     6121 agtatcttta ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat
     6181 tgccaattgt cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc
     6241 cataaaaaac tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta
     6301 ctgctatcat agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag
     6361 ggtttaaatg gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc
     6421 tagtacagaa caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga
     6481 gactatacga cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt
     6541 tttaggaact tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc
     6601 aactcaacta aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta
     6661 tcagcacaca aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat
     6721 agaaacatac ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga
     6781 tatgttgttt gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat
     6841 tactactttt tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg
     6901 acaattgtct ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt
     6961 aattggaact ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc
     7021 tgacgatagt cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt
     7081 ctctactgaa aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa
     7141 ttatgcattt aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc
     7201 gttgttagtg gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc
     7261 actatctaat ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg
     7321 tcttcgtacc ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt
     7381 agtaatgtcc agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa
     7441 atattctgag gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga
     7501 tcgtgaacca atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat
     7561 tccggatatt ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga
     7621 actctggacc ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc
     7681 tattaaacat actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac
     7741 taataaatgt gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga
     7801 tatttatatt cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa
     7861 aacttgggtt caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa
     7921 tgaaatgtct ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg
     7981 ttggcctact tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact
     8041 taaaaagaca tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact
     8101 tcctaaaatt gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca
     8161 atcatttaac cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt
     8221 agcagttctt ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt
     8281 tgattcttta aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac
     8341 actttcagga gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat
     8401 atggggtgat gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc
     8461 cctatattct agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa
     8521 ttttgtctct gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga
     8581 ttcgctttac cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat
     8641 catgactgat acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt
     8701 tcaatttagt ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa
     8761 cgctcactac ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta
     8821 tacccattgg gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc
     8881 aacagaatta acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat
     8941 aactgagtat ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat
     9001 atatgcacgg caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg
     9061 taatattttt tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga
     9121 gcagctcaat gcattaggat tagttactaa tgccatcata tattggaact cactgtattt
     9181 agaaaaagta tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg
     9241 caggcaatct ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata
     9301 cgaagctgaa ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg
     9361 ttcgggtttt gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat
     9421 agaaaaagag aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg
     9481 tgctaccgca ctatttcgtt tctatgttcc atgtacccca aaatcggtaa aagagaaggc
     9541 aatcaagaga actttattac gaatatcaaa aaattcattg ttcatttact ggaagctgta
     9601 cctaacgata tagaaaaact aaatttttct gattaccagg aacagaaaga aaaagaagca
     9661 gaaaaaagta ttgtaggaaa atgtcctaag tgtggcaaca atattgtatt aaaaaaatcg
     9721 ttttatggtt gttcaaatta tcctgaatgt aagtttactt tagctgaaca ttttagaaag
     9781 aaaaaactaa ccaaaacgaa tgtaaaagaa ttactggagg gaaaagaaac cctggtaaaa
     9841 ggaatcaaaa acaaagagaa aaagccctac aatgccgttg taaaaattgg ggaaaaggga
     9901 tatattgatt ttatatcttt ctcaaaataa a
//
LOCUS       DAJSVF010000097         9911 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSVF010000097 DAJSVF010000000
VERSION     DAJSVF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.100"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            5..361
                     /locus_tag="ON550_002390"
     CDS             5..361
                     /locus_tag="ON550_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HCR3955425.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            377..727
                     /locus_tag="ON550_002391"
     CDS             377..727
                     /locus_tag="ON550_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HCR3955426.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            756..1535
                     /locus_tag="ON550_002392"
     CDS             756..1535
                     /locus_tag="ON550_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HCR3955427.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1546..2223
                     /locus_tag="ON550_002393"
     CDS             1546..2223
                     /locus_tag="ON550_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HCR3955428.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2242..3348
                     /locus_tag="ON550_002394"
     CDS             2242..3348
                     /locus_tag="ON550_002394"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HCR3955429.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3338..4429
                     /locus_tag="ON550_002395"
     CDS             3338..4429
                     /locus_tag="ON550_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HCR3955430.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4442..5182
                     /locus_tag="ON550_002396"
     CDS             4442..5182
                     /locus_tag="ON550_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HCR3955431.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5470..7410
                     /locus_tag="ON550_002397"
     CDS             5470..7410
                     /locus_tag="ON550_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCR3955432.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7497..9419
                     /locus_tag="ON550_002398"
     CDS             7497..9419
                     /locus_tag="ON550_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR3955433.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9699..>9911)
                     /locus_tag="ON550_002399"
     CDS             complement(9699..>9911)
                     /locus_tag="ON550_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3955434.1"
                     /translation="FTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPSQAVGSEEE
                     ILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3233 a         1643 c         2204 g         2831 t
ORIGIN      
        1 aatcatgatt aaagtagtta ttgcagaccg tatgggtaaa ggacaaaatg tggccaaagg
       61 ggtagaagca gctggtggaa aagcagttgt cgtaccagga atgggggcag atatgcgttt
      121 gggcgatgtg atgcaacaag agcatgcaga tatgggtatc tctttttgtg gtagtggtgg
      181 tgcaggtgca ttaacagcag caacaaaata tggctatcca gaacgacatg gtatgcgttc
      241 gattgaagaa gggatcacag ctatcaagga tggcaaaact gttctaggtt tcggctttat
      301 ggatcaagag gaattaggga aacgtctgac agaagaattt atcaaaaagc atggaaaata
      361 gaaagaggga catgaaatga gcgttcaaaa gacaattcag caaaaagtta tcgtctccgg
      421 taaaggggaa acaaagcaac atgcttttgc atcagcgtta gcagaaattc aaaacaaaat
      481 aatggaacaa tcaaacgtat tattgcgaat cgaaccagtc aatgtggata ttttaaaggc
      541 ggtcgaaaac aaatacacag aacgctttct atttttcttc ttcccacggc aacgtattag
      601 ctatgaagtc gaactctctg ttactgtggc tgtaacagaa ataaaattgg cagacatatc
      661 gttcagccag caaatgactg aagatccaaa tgggattgcc attccatttg taactaaaaa
      721 aatctaattt tgaatcataa gggggaaaga ataacgtgga tttcgtaatc attctagtca
      781 aatcggtcat tatcggggga ttacttggtt ttgcagcatc tgtcggggca gctcgaatgt
      841 ttcatgcacc taatacacaa ggtttaggag cttttagaac attaggcgaa atgaatgcct
      901 gcatgggtga tccagcttct catttttcat ttggtttagg ctttttcttt aatgcatggg
      961 cttcttcagt tggtgctggt gcattcacac aagatgtgac gcatcgaatc attcccaatt
     1021 gggcagcggc agcacttcta gcaaaaaaca aagatgtcac ccagaccatg catgatccga
     1081 aaaaaatggg gatcattgga gcggttatcg gggcagtggt ggtagccttc ttaaacacaa
     1141 cagctgcagc aattccagag gcgttacaag tgacggcggt ttccgttttg gtaccagcag
     1201 cgacgattct gatcaataca gtaatgccag ttattttttg gttggcagct ttagacgcag
     1261 ggaaacgaac tggtttttgg ggcactcttt ttggtggtat cgcgcaattg attatgggaa
     1321 atgcggtacc tggtgttgtt ctaggaatct tgattggtaa aggcgtcgat gagagcggtt
     1381 ggaatcgcgt cacgaagagc atgatgatcg ctattgtttt attatttatt ttcagtggat
     1441 ttttccgtgg atttgatatg gaaatgatca agagcttcca gcttcaagtg cctaattggc
     1501 ttgataactt gcacaacatc tttagtgtga aataggagga gaatcatgga gacaacagaa
     1561 gaacaagcaa aagtattaga agcaaagcgg caaaagagct tttggtttgc agactggtct
     1621 tttccgctaa tcgtaggaat tatgtccgct gctgtatttg caggaacaca catgtatgtg
     1681 atgtatggtg tgggtgcatt caatgaagtt tcgatcgtgg cgatgttaaa agccgggctg
     1741 gatggcggat cctatggcgc agcggcagcg tttggtgcta gtttcttatt tgccagaatt
     1801 ttagaaggct ctttagttgg gatcttggac ttaggcggtt cgatcctaac tgggatcgga
     1861 attggggtac cagcaattct cttaagtatg aaaatagcgg caccaatcga taattttgca
     1921 ctctctttat tgactggttt ggttctaggt ttgattgttg gtggcatcat tgtcttgatt
     1981 cgtaaattta cgatcaatca atcaaattcc acttttggtg ctgacgtcat gatgggggca
     2041 ggaaattcat ctggacgttt tttgggtcca ttaattatat taagcgcgtg tgcagcatct
     2101 attccaatcg gcattggcgc tacattagga gcactaggat tctatgcttg gaaaaaacca
     2161 attgctggag gtgctatttt aggagcaatg attttaggag caatcttccc ggcagtaaat
     2221 taatagaaag agggtcatta aatgtttgat actttgatta aaaatggaag gttagttgat
     2281 ggatctaaaa tagaggtcgc tatagaaaaa ggcatgatta aagcagttgc agcagaaata
     2341 aatcagccgg cgcaagaagt agttgacttg gagggaaagt attatttatc cgctgggtgg
     2401 atcgatgatc atgtccactg ctatgaaaag atgaatcttt attatgacta tcctgatcag
     2461 ataggggtcg aaaaaggtgt gacgacggtc attgatgctg gaactacggg agcagaaaac
     2521 atccgtgaat tttatcaatt gacgaaatcg gtcaagacaa atgtgtatgc gctaatgaat
     2581 atctctaaat ggggcattgt caaacaagat gaacttgctg atctaacaaa gattcaagaa
     2641 gatctagtaa gtactgcgtt agcagaactg ccggaattta ttgtcgggat taaagcgaga
     2701 atgagtaaga cagttgttgg tgaaaatggc attaagccat tagaattagc taaaaaaatc
     2761 cagacagaaa acaatgatct gccattgatg gttcatatcg gttctgcacc tcctgagtta
     2821 gaagaagtat tgtcaagaat ggaaagaggc gatgtattga cccattgttt taatggaaaa
     2881 ccaaacggta ttttagatcc aaagacagat caaattaaaa actttgtctg ggatgcttac
     2941 aataatggtg tcgtctttga tattggacat gggacggata gctttaattt ccatgtggca
     3001 gaaaccgcac taaaagcagg aatgaaagct acctctatta gtacggatat ctacatccgc
     3061 aatcgtacaa atggcccagt ttttgatcta gcaactactt tagaaaaatt gagggtagtc
     3121 ggctattctt gggaagaaat cattgaaaaa gttacagcag ttcctgcacg gaattttcat
     3181 ttgaagaata agggccagtt agctgtcaat tatgatgcag atttgacgat atttgagatt
     3241 gttgaaaatg aaaaggaatt gaaagattcc aacggattta cacgtatagc taaagaacaa
     3301 attttaccga ttaaaacaat tattggaggc gtcatatatg acaattagct atggaaagtt
     3361 tgatttaaaa gaagtcatta atgcatcggg aaaaatgacg atcctagggg tttctaaggt
     3421 atctgaaagt gtcctagcag cacaacgatt tggaggcgag catttttttg agatgagtga
     3481 tcttgccatc aaaaccggcg cttatctttc tcaattactg aaagtggaag atgcgcaaat
     3541 cgtttcttct gcttcagcag ggattgctca atctgttgct gctttaatcg gacagggcga
     3601 catgtaccat gtctatcatc cctatacaga aaaaatcgct agacgggaaa tcatcatacc
     3661 taagggacat aatgtagatt atgggacacc ggttgaagta atggttgctc aaggtggagg
     3721 aaaagtcata gaagctggct atgcaaatat gtgtacaccg gaacatgttt ccatgatgat
     3781 tacggaaaaa actgctgcgc ttctatatat taaaagtcat catgcagttc agaaaagtat
     3841 gctgacggtg gcagaaatgg tagaagttgc caaagctcat cgtttgccat tgatcgttga
     3901 tgcagctgcg gaagaagatt tattcaaata tagcgaaatg ggcgttgact tagtgattta
     3961 ctctggggca aaagcaatcg aaggtccgag tgccggactg gtcataggaa aaaagaagta
     4021 tattcaatgg attcggctac agtctaaagg tattggccga gcaatgaaaa tcggtaaaga
     4081 taatatttta ggctttaccc aggcactaga agattattta aaaaatggga gcgaatccgg
     4141 tgactccatg aagcaacgat tagcgccttt tgttaacgca atcaatcaac ttcctgacct
     4201 ttcagcgaaa atagttcaag atggtgccgg cagagatatt tatcgagcaa gtgtaacgat
     4261 taatggcgat aaaagtgcga aagaagtcat tcaggaatta aaagcacgaa atccagcagt
     4321 ctacacccga gaatatcaag caaataacgg aatcattgag ttcgacattc gttccgtcag
     4381 ccaaaacgaa atggaaaaaa ttataaaaag attgagagaa atcatgtagg aggcttttaa
     4441 attgtcagtt atcccaaatt attataaaaa tcgtgtatgt ttaaatgtat tagctcattc
     4501 agttgaaaat gcaaaagcat gctatgaagc agcggaaggg catgtcgtac taggtgtgct
     4561 atcgaaaaat tatgctagcg atgaagcagc aatcgaagat atgaaaaggt accaagacgc
     4621 aaccaataat gcgttgtcag ttggattagg cgctggagat ccaaaccaaa gtcagatggt
     4681 ttcgcgttta tctggggttt tacaaccaca acatgtcaat caagtattta ctggcgtggg
     4741 gacttctcgt gctttattgg gccaaaatga tacagtcatc aatggattgg tttcaccaac
     4801 aggtaagatc ggctatgtca atattgctac aggtccgtta agttcacaag cgccagcagc
     4861 tgaagtgcct attgaaactg ccattcaaat gctgcaagac atgggaggta cttcaatcaa
     4921 gtatttcccg atgaaaggct tagcacatag ggaagaattt aaagcagttg cggaagcttg
     4981 cgcaaaatat gaattctatt tagagccaac aggaggaatt gatctttcaa actttgaaga
     5041 aattctgaaa attgcaatcg atgcaggtgt aaaaaaagtg attcctcatg tttatagttc
     5101 aattatagat aaagaaacag gagatacgcg tataggagac gtaaagatac tattaagcat
     5161 ggtaaaaaaa attattcact aactcattag aaaataagaa gcttattcca gaatgaaatt
     5221 aaatggaata agctttttat aattaattac ggaaattgtt aatgtagata ataatttaga
     5281 tgtacgtaag cagaagtaca gaaatgtaag attttccaat attattttat tcaacataat
     5341 aaatcgtata tcaatttttt tagcgaaatt tctatttatt ccaagaattt cagttattac
     5401 ggaatcattt tgtgcttaat aaaaagaaac cgtttttata tactataaat gtagaaaggc
     5461 ggtaggaaaa tgaaaaaaat tattgcaatc acatcctgtc cagttggtat tgcccataca
     5521 tacatggcag cagaaaactt agagaaagcg gggaaagcag ttggcgccga ggtaaaagtc
     5581 gaaacacatg gttccatcgg tattgaaaat gaattgactg caagagatat cgaagaagca
     5641 gctggtgtaa tcatcgcagc agatactaaa atagataaat cacgattcgg cggtaaacct
     5701 ttgataaccg taggtgtgca agaaggaatc caccatgcag atgagttggt gagagatatt
     5761 ttagcaggaa aagcgccagt ctataaaagt acggaggcaa ttatttcttc cgaaaataaa
     5821 agtgaatcag aaaatttagg taaaaaaatc tataagagtt tgatgaatgg cgtttcgtac
     5881 atggtacctt tcgtagttac aggagggtta ttgatcgcga tttctttaac attaggagga
     5941 acagctacac ctgaagggat caagatacca gaaggaacga tttgggcaac gatgaatagt
     6001 atcggaagta tcgctatggg gttgatggtt ccaattctat ctgcatttat tgcgcaaagt
     6061 attgcagatc gtccaggact tgttcctgga tttgttggtg ggatgttagc ggcaaacgga
     6121 gcattatatg gtagtgatgc gaatgcggga ttcttaggcg ggatcatcac tggattttta
     6181 gcaggattca ttgctttagg aatcaagaaa gtgcgagtac cgaaagcttt acaatcgatc
     6241 atgccaatta ttgttatccc aattttagga tcattggcag ttggttttgt gtttatctat
     6301 gttattggag aaccagtagc tcttctattt agttcattga ctcacttttt agcagggatg
     6361 caaggaggaa gtgaaattgt tctagctatg atcttaggag cgatgattgc ttttgatatg
     6421 ggggggccgt tcaataaagt agcctttttg ttcggatctg gcttgattgc agaaggcaat
     6481 tattccatta tggggccaat tgctgtagct atctgtatcc ctccattagc attggggctg
     6541 gcatcattga ttttaaaaac caaatttacg aaaacagaac gagaagctgg taaggcgtcc
     6601 ttagcaatgg gactgtttgg gattacagaa ggcgctattc cgtttgcttc aacagatcca
     6661 ctacgggtga ttccaagcat tgtaatcggg gcaatgacag gaagtgtgat cgcgatgctt
     6721 tcaggagtga cagatcatgt agctcatggc ggtccgatcg tagcggtttt aggagcagtg
     6781 gatcacgtag ccatgttttt tgtagcagtg attgctggta ttgctgtaac cgtgctaatg
     6841 ttaagaatcc ttaaaccgac attacaacaa actgcggttg ctgcagcaaa tggtgtgaca
     6901 gtagcagatc tatcagagac tgaaacaagt gatgtttata cgaaaactga aacttttgat
     6961 tctatcacgg atattatgtc agatgaagca atcgttttag gtgaaacagc aacaacaaaa
     7021 gacggagtaa tcgaagattt agtccaacgt ttagaaaaaa cagcggcgat cacagataaa
     7081 accagattta aagaagtcat ctatgataga gaacatgaaa gtacaacggg aatcggtatg
     7141 gggattgcta ttccacatgg aaaatcagct tatgttctta aaccaacagt ggcatttgcc
     7201 agaagtcaat ctggggtaga ttggcattct ttagatggaa aacctgcaca tatgatcttt
     7261 atgattgctg tacctgaaaa cagccaaggt gatatgcact tgaaaatact gcaacgtcta
     7321 tcacgtaaat tgatggatga tgacttccgt caagcactga tggaagcgcc agataaaaca
     7381 acagttcacc aattattaag cgaaatgtaa cataagatga aaaatcgacc gtgatgagac
     7441 ataatagatg tgttatcatg atcgattttt tatttagaag ggagtttctg cacattatga
     7501 atcagcgtca gcaatctttg atctatcaat taagtgagaa caataattat ataacaacaa
     7561 aaaagctagc agagcatttc catgtgtcag aaagaacgat tcatagcgat ttgaatacca
     7621 ttgaaacttg gttgagagac aaacatctgc ctttaatgtt tgaaagaaaa aaaggtacag
     7681 gtatcttgtt gaatggtctg cctcacgaaa gggaacaact gaaacgagcg cttaatgaag
     7741 agactcaaac agaaatggat aaaaaacagc ttcgtcagtt attgattttc catctgctag
     7801 tagcaaaaga cggatttagt cttgaagaac tttcagaaaa actatatgtt ggaaaacgta
     7861 cgattcgtaa agaattgaca gagttgcaaa gcttttttga gtatcaccat ttacagctag
     7921 tatccaaaac aaaactagga acttttctta aaggcgatga acaagaaaaa agacagttgc
     7981 ttgttaagac attacgaaat atgcagaaag aagatccaca aagtccgtcg ttgaaagaat
     8041 tcttcgctaa agatacatta aaagttatcc aacatacatt acaagatgtt ttttatgaaa
     8101 accatttaga acagccgaat ggactggcta gtgtggagat tcacatctac tttatgctcg
     8161 agcgaatgaa acagtaccag aaagtgaaat taagtaaaga cgaaaacgaa gtcgtggaac
     8221 acacccaagc acaaaagtta agtagtcaaa tattagcaaa actagccacc atctatccaa
     8281 ttgaattttc tcctgatgaa atcaattatc tggctttgcg tatcgccaat ctttttacaa
     8341 attcacaagc agccacacgt tttcaaaagg aatctagcgc acttgctgat catttgatcg
     8401 aacaagtgga acagtttcta ggctattcat taaaagaaga tcagctacta aaacaaaatt
     8461 taaggtctca tttatcttca acttattttc gactccatta tggtttgcag atttctaatc
     8521 cattgacgaa aaatgtgttt agtacgtaca cacaactttt cttagtttta cagttaattt
     8581 tagaagatta ttttgaaaag gaaaattttt atgttccaca agaggagaca gcttatttaa
     8641 cgatccattt ccaagcagcg atcgagcgac acaaacatca gaaaagtaga agatatcaaa
     8701 cggttttagt tagtgaatat tcaaaagcta tggctacttt tctcgaagca cggctgaaca
     8761 gagaacttcc agaattaacg attatggatc tagttgaata taagccagaa ttaaagagac
     8821 aagattttcc ctctgttgat tttatcttgg ctacgttacc atttaaacat ataagcattc
     8881 cagtaatcga gatctcacca atgatcacag aaactgatct agcctatttg acaaaatata
     8941 tgctggagca tgtgcctatc aagaagaaaa aaacatttga tctagcaagc tttactcatc
     9001 catttctgat ctttcctcaa cttgaacgga cagacccagc cgatatttta aactttatgg
     9061 gaaacgtctt agtggaacat cattatgtgg agccggaatt catcgattct gtcttggaaa
     9121 gagacagtca ctccagtact agagtagcac catttgtaac aatccctcac ggtaatccgc
     9181 tgtatgtgaa gcattcgatg atttctattg ctacaatgaa agaaccagtc ctatggcacg
     9241 gggaacagat acgtatcgta ctgctattag caataaagaa aaatgatttg aaacatgcag
     9301 agttcaaaaa aatattttct gttattcact atctagaaaa gcagccagaa caaatggctc
     9361 agttgatgca atccaatcat ccgctagaaa tcttaacgtt gttatctagt tatgaataag
     9421 tagcgattaa aaaactgcct ttatatttat aataattcta tctatttatt tggaagtaga
     9481 aaactaaata caaaaaaaga ggatgtggcc aaagttgtgg cacatccttt tattttgagt
     9541 ttcttataat tttttatagt tgcttacgat gtcttgacta ctggtaaacg gtgttcaaga
     9601 gacaatttgt cggcaataag gcacaatttt gtaaaatatg gaaagctatt tttttaacat
     9661 tgtttcttag tgcttgaaaa atcattttga atacttcttt atacgatcga tgtacgttga
     9721 tttaagtcta aaacttttct tttgattcct tctctgactt ttctgaattc cgttaatatt
     9781 tcttcttcgc tcccgacagc ttgtgaagga tcttttaggt cccagtgaag gtgttcgtga
     9841 ttgtttggaa cggcagggca cttgtctttg gcatcaccac aaagtgtaat gatcaagtct
     9901 gcctgagtaa a
//
LOCUS       DAJSVF010000098         9852 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSVF010000098 DAJSVF010000000
VERSION     DAJSVF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.101"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            56..127
                     /locus_tag="ON550_002400"
     tRNA            56..127
                     /locus_tag="ON550_002400"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Gln,seq:ttg)
     gene            145..229
                     /locus_tag="ON550_002401"
     tRNA            145..229
                     /locus_tag="ON550_002401"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:179..181,aa:Leu,seq:caa)
     gene            484..1359
                     /locus_tag="ON550_002402"
     CDS             484..1359
                     /locus_tag="ON550_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3955435.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1463..2227
                     /locus_tag="ON550_002403"
     CDS             1463..2227
                     /locus_tag="ON550_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR3955436.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2214..3656
                     /locus_tag="ON550_002404"
     CDS             2214..3656
                     /locus_tag="ON550_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3955437.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3667..4998
                     /locus_tag="ON550_002405"
     CDS             3667..4998
                     /locus_tag="ON550_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR3955438.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            5011..5469
                     /locus_tag="ON550_002406"
     CDS             5011..5469
                     /locus_tag="ON550_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3955439.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EETLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            5761..7062
                     /locus_tag="ON550_002407"
     CDS             5761..7062
                     /locus_tag="ON550_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3955440.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            7089..7349
                     /locus_tag="ON550_002408"
     CDS             7089..7349
                     /locus_tag="ON550_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3955441.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(8348..9583)
                     /locus_tag="ON550_002409"
     CDS             complement(8348..9583)
                     /locus_tag="ON550_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3955442.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3100 a         1511 c         2057 g         3184 t
ORIGIN      
        1 cggattgtgg ctccggcatt cgtgggttcg attcccatca ttcgcccttt attcttgggg
       61 tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct
      121 accccagtta ttatttttaa atttgccggc gtggcggaat tggcagacgc gctggactca
      181 aaatccagtg tccgttgagg acgtgccggt tcgaccccgg ccgccggtat aattatcaat
      241 atagcaactt tcttatagag agttgctata ttttttttgt ttttatgtaa ttacctccaa
      301 atcaattcga tacaagtatt atcataaaac gagtaaaaaa gatattgata acgcttttat
      361 tatgtgttaa actatttttg taaattatgt agggtgctac tattcaatta ggcacgacct
      421 aagaaagggt tctttcttag gtcgtttttt gtctttaaaa attgtttgga gacaaaaata
      481 tgtatgaagg agtctgcaag ttgttttgtt ttatcatata gaggaggtga attaacggtg
      541 tatgtgatga aaaagctgaa tcataatgct gtattgatcg atgacgatgg aatagaaaaa
      601 attgcgatgg ggaaagggat tggcttttct gctaaagtga gtaagccatt tgatgttgag
      661 gatgctgaca aattatttgt attagattct aaagaaaaga tcaaacgatt cagtgagata
      721 gtctcgcaga ttccaatcga atttatggat tttgcggaag aagtgattga actgataaca
      781 gctaggatct cctctccatt agattcaaat atctatatta cattgacaga tcatatttat
      841 tttgctattc aacgaaaaag agaagataga gatgtcacag caattatgtt gccagaaatg
      901 agattccttt atccaaatga atatgatata gcagtagaag tggtcgagcg aatcaatcat
      961 cgatatcaaa ccgagttagg gtcaaatgaa gtaggattca tcactatgca tatcattaat
     1021 gctgagctag gagagcctaa tagtttaaag agcttaaaaa tagtagaaat gacgaccacg
     1081 attttgtccc atctagaaaa atcctattca aacgtttatg ataaagaatc actgacttat
     1141 catcgcttaa tggttcatat caaattcctg gttaaacgct tgatttatca ggaagaaacg
     1201 ccagtagaaa atattggttt ttttactgaa acatttaaac atagcaaagc gtattgtaca
     1261 gcactaaaga ttaaagatat catcgaacag caatttcaac ttgccattca agaaaatgaa
     1321 atcatctatt tggcaattca tctagcaaga atctattaaa ataagggcgt aactatctat
     1381 aggcgcaacc aaatgacaca tagtaaatgg actatgatta tttggttgcg cctattttag
     1441 tagaaaaaag aaggagaatg aaatgggaaa agtgattttt aattcggatg attttggcta
     1501 tagccatggt gtgaattatg ggattatgga tgcttatcag cgaggaattt tgacatcaac
     1561 tacattgatg gcaaatatgc cgggtttcga gcatgctgtg aaactaagaa aggaaatgcc
     1621 taggctaggt gtaggagtcc atttaacatt gacgtgtggc aaaccactcc tcaaaactgt
     1681 cgatactctt atcgaaagcg gagagttcag atccttatct tattatcagc atccatttaa
     1741 cattgacaaa gatcaactgt atcaggaatg gaatgctcag attcaaaaaa tttatcatgc
     1801 ggggatcata ccaactcatt tagacagtca tcatcatact catactttgg atgaaaatca
     1861 ggaggtagtt gttacattag caaaaaaata tgatttgccg gttcggagca actttgaacg
     1921 aaaagatgaa gttcgtcatg tcgattattt tgagccttgt ttcgatgtcg taggagaaac
     1981 tgatgaaaaa aaacgacaaa tcaatacgag tgtggaagaa tatttggcaa gtttactcat
     2041 gatattgaga gaagataaaa cgatcgaagt catgtgtcac gcagcgtatt tggatcagga
     2101 attgttaaat ggttcaagtt ttgtgtatcc tcgtacccac caagtcgaat ttttaattca
     2161 ttcagcattt gctcaaaaag tgaaaaatga tcctttgatt gagttagtaa catatgagga
     2221 tatttaatag aggaggaata aaaatgaatg ttaaatcaaa tgtgcagaag cttggaagag
     2281 cgatgctact tccagttgca gcgatgccat tagcgggatt gatcatgcgt ttatcagctg
     2341 acgatatgct gaacatccca gtaattggtg cagctggaaa tgccgttttt ggaaacttgg
     2401 atatcttatt tgctatagga gttgcaatcg gttttgtcaa aaataaggac aaaggaatac
     2461 ctgcacttac tggtttttta gcgattgcta ccttgaaaaa aggcttagaa attatggacc
     2521 cagaagtgaa tatggggatt tttggtggaa ttatttccgg tttagtagcg gcatggacgt
     2581 ataatcgctt taaagaacaa aaattaccag tagttttttc ttattttgcg ggcgaaaaat
     2641 tcccattgac tatggtgatg attctacaaa caatcgcagc tgtttttttg ggctttatct
     2701 ggccgctcat ccaaattggg attgataatt ttgcaaaact gctcgttgat atgggagcat
     2761 taggtgtggg gatatttatg tttttgaatc gtttgctgat cccctttgga ctgcaccatg
     2821 tgttgaatac gtatatttat tacgatttag gaagctatac atctcctagc aaggaagttt
     2881 tcagaggaga gatgacacgc tttatcaacg gagatcctca ggctggactg tttttatcag
     2941 gtttttttgt agtaatgatg tttggtatac cagctatatg tctagctatc tataaagcag
     3001 cttataaaga aaataaagag atggtcaaag gaatcatggg aagcggtgca ggaacttcat
     3061 ttattgcaaa tatcactgag cctgttgaat ttagtttcat gtttctttct cctatgttgt
     3121 acgtgatcca tgcacttttt gccggtttag cagggattgt ctgttactta ttcaatatac
     3181 gaattggttt tacattcgga gcatgtattg ttgattatct catcaatttt cgaatcgcta
     3241 ccaatgctat tttgattctt ccaatcggaa ttttcttttt tgccttgtat tacgttactt
     3301 tttattattt gatcaataag agaaacattc agacattagg acgtgaagct aaggcagagt
     3361 ttggcaatga agtaacctta gaagaaacag agttagggtt agcaagtaaa aattatgaat
     3421 atatggcaac aaaaatgcta caagcgtttg gaggaaaagc caatatcctg gatgtctata
     3481 gttgtaatac acgcctacgg gtagaggtag tggatcccac catggtcgag gaacagcgaa
     3541 tcaagcaatt agggataagc gggatcatta aaccaactga aaaaaattat caaattatca
     3601 tcggcttaga agtgacttat gttatggcag aattcaataa attattagaa gaatagaaag
     3661 gaaaagatga tgatgaaaaa acagattatc actatagctg gaggcgggag tacgtataca
     3721 ccaggaatcg ttcaagccat tttgaacaat gaaaaacggt tgccattatc tgaaatacgt
     3781 ttatatgata ttgatgaaga aagaaatatg gatatgtact tgattgttaa atttatgctg
     3841 aaaagaaaag gtttttcaaa tattcgcatt cgtgcaacgg atgatccaaa gcttgctttt
     3901 acaggatgcg attttgtttt ctcacaaatt cgtgttggag gattggagat gcgagaaaag
     3961 gacgaaaaga ttccacttaa acatggatta gtaggacaag aaacgtgtgg actcggagga
     4021 tttgcttatg ggatgcgctc aatcaaaggt ttattggaaa ttgtcgatca tatacaagac
     4081 tatgccccca aagcatggat tttgaattat acaaatccag agtcgatcgt ttctgaagct
     4141 gttcgtcgta aatatccgaa tgtaaaaatg attaatgcct gtgatatgac tatttcaatt
     4201 gaagaaacga ttgcagtgaa ttacggttat gatcgtaaga attggatcgt tacgtattat
     4261 ggtttaaacc actttggctg gtatacatcg atttatgata aagaattgca acgagaaatt
     4321 atgccagaga ttattgaaaa actaataaca aaagaaatgc aagtcgccga ttttaatata
     4381 ggagataaaa cttggcaaaa aacctttcaa atgatgtcag tcatcacgaa aaattttcca
     4441 agtaatatcc cgaataatta cttagagtat tacttatatc ctgatatggt cgttgagcat
     4501 actgacaaag agtatacacg tgctaatatg gtcatggatg gacgagagaa aaataccaaa
     4561 gaaatggctg ataaaatacg ccgcggtatt gtggaagaag tattgaactt taattttggg
     4621 gagcatggac aatatatcgt cgatatagct atttccttgt tgaatgatga tcgtcgtcgc
     4681 tttatgctaa tcgttcctaa tcaaggggcg attccgaatc tacggtcaga tgcagttgtt
     4741 gaaataccgg catatgttgg tgcaactggt gtggagccaa ttacactaag aaaaccaatc
     4801 agtgattttc ataaaggatt gatggaggca caagttgcag tagagaagtt actagtagat
     4861 gcttactttg aaggttctta tcaaaaagct ttacaagcat ttacattgaa tcaaaccgta
     4921 ccaaatgcca gagtcgcaaa aaaaatacta gatgaaatga tcaaagtaaa caaaaaatat
     4981 tggccagcat taaagtagag gtgataaaaa gtgcatttct ttaaaacaag aaacattcta
     5041 aaagctgtag ctagtggtca aattattcca ttaacagcag taaaagacga ggtcttttct
     5101 tcaaaaatga taggagttgg atacgctata acgaatcatg atggatatat ccatgcgcca
     5161 gttaaaggaa taatcgataa tatatttcct acccgacatg ccataacgat cgaaagtgaa
     5221 tctggcagaa aaatactgat ccatatgggt ttagatacag ttgaactcga aggaagtccg
     5281 tttcagatac gggttgaaag taaccaggag gtaaaagaag agacactgtt agctattata
     5341 gatttgaaga agttggagca agcacaaaaa gatacggtga tcattgtagt ttctccaggc
     5401 atttttgaag gaaatttatt aaaacaaaac caagaagtaa agattaaaga tagttgtttt
     5461 gaattttagt gtacgatgaa aaaagttaaa tagggtgata tttatgggtg aatttacaaa
     5521 atttttttga aatgatatgt ttcaatcact agatttaatc attttgattt ttgttaatta
     5581 tcgaattttt ttgttgctcc tatgatttag aaaacttatt ttcaggattc ttaaaaataa
     5641 atttgattat ttaagagaat atattgaaat aaaatttctt aattatataa ttagttatga
     5701 acattctttg cgttgtcatg caatgaatgg ttcatatttt attgaaaagg agtggtttta
     5761 gtggaaaagc tgatgaaatg ggtcgaagag aaacttgtcc caccaatggc aaaaattggt
     5821 acacagcgtc atttattagc aattcgtaat ggggttgttt caacattgtc attgattttg
     5881 attgggacat tttttatggt ctttatcaat ttaccgtttc ctggatggaa tgaatttatt
     5941 gccccttatt cggcaacgat tgttttaccc tttaggatga cgatgggatt gatggctatt
     6001 tatgcaactt ttgttatggg ttcggatcta gctaaatcat atggattaga tagtgtgaca
     6061 ggtggtatat tatctttagg aacgttcttt atgttacaag ttcctgtaaa cgttttaact
     6121 cctgaagagg cacctcttgg ctgggtctta cctatgagtt ctttgggagc atctgggatg
     6181 tttgcgggga tcttatctat gatttttgca gtggaagtat atcgtttctt taaaaatcga
     6241 aatattacta tcaaaatgcc agaacaagtg ccacctgctg ttgcccgtag ttttgaggca
     6301 ttgattccag gtgcagtcat tttaacaatt acttggctga ttcgaagcgt cgcgggcttt
     6361 gatgtaaacg ctgccttatt atcgttattc gagcctttaa ccaatatttt agggaataat
     6421 ttgcttggtg ttttactacc aatgtttttg atccatttgt tgtggtcatt tggtatccat
     6481 ggcatgagta ttgtaggggc tgttgttcgt ccaatgtggc tgatcatgtt ggatgagaat
     6541 gcaaaggcat tggcagatgg tacagctgcc acccaattac cctatatcgc tcctgaacag
     6601 ttttatcaat ggacggtaac aattggtggt gcaggagcaa cgatcgtggt atctgttttg
     6661 tttatattct tctgtaaatc aaaattcttg aaagaggttg gtcgtttttc tattattcct
     6721 ggtatcttca atatcaatga gcccataatt tttggagctc ctatgatgtt gaatccttac
     6781 atgttcatcc cgttcaactt agttccttta gttttgacta ttatcagtta ttttgcagtc
     6841 aaattagaaa tggtcaatag atttactgtt ttgccaacat ggacattacc agctccaatc
     6901 ggcggttatc tttcagcagg aaatgactgg cgtgttgtta tcttgatcat gatcaacaca
     6961 ttgatagcat ttattatcta ttatccattt gtaaaagctt atgataaaaa gatgctgtca
     7021 gatgaacaaa gtcgtggaga aaacgaagtc cagactgtct aagtttggtt tttagaaggt
     7081 gggaaacaat gtattggctg attttatttt ttgtttttat ctttttatta acggctagtc
     7141 accttatatt gaatatgcta gctacatatc atattcaaat caatcgatgg atttgggcac
     7201 tggcatcatt tttgatcgtg attcttccaa agatcattgt accgcatatg aatgttttgt
     7261 tttcatgggg gacatatgta ctttgtggaa tttttgctat caattttatg atcgagcagc
     7321 atagatggtt tgtcacatcg aagctttaaa tgttctaaaa gtggttgtaa cagaagtggt
     7381 tagcttcaag aaataagaag aaatttacga aaattgcttc ttaaattttt gtgaaatgtt
     7441 ggcttatttt tcgtttggtt gaacttatta ccgcagatgc aagcttttga ccaccgttta
     7501 ttcggaatat gggactgtga caaggctttt gtcacagccc ttttttacat aaagattatt
     7561 attgttttaa ctgttttggc agaaagaagg aggaaaaaat ggaaatttta tcagcaatgg
     7621 atttagaatt agtagatcta ctaccaaagt gatcaagtca aatatagatt tgggagctta
     7681 caaccatata gtaggggctg cagtatggtg tatgaatggt aatatgtatg atgtgagtgt
     7741 ttattcaaat catgaaacgt gtatagaaat aattgtgctg ggaatagcaa tcagtcatgg
     7801 tgaacgtgac ttttagtgta tcgttgctga cgacatagaa cattctgaca tgatctactc
     7861 aatatgtggg gattgctggc agtttatata ggagtatgca tcggattgcg agatcattgt
     7921 ctcaacagaa gagggaggtt gattatcaaa gataaagtgg taaatcctac atttgattta
     7981 gtaattgttg aggggactag cggcacaaat ctctacaaag cgatgcttct agatctatat
     8041 gacacatttt aagaaaatgt atttgcctaa ttattatggt ctttcttttg aagaaacgtt
     8101 gagatgatcc aaccagagaa aggtgtcaaa gaatttgact aagaataaat ggcagacgct
     8161 acctatgctc atttggatag cgtcagatct tttattttaa ggatgatttt gtgtttttta
     8221 gacaccgtaa tttaagtaac tctaactttt caatccacta tttacttttt ttgtattttc
     8281 actgtctggg tctcgaatag cgaaattact gatgagtctc tattaaaaaa gctttttcta
     8341 cttgaaacta tattgggtgg ttgctcagga aggtttcgat ttcttgaatt gcttgttgtg
     8401 gaccatttat tggaaaaacg gcttgcagcg tgcgtgcctg ctgacgaatc ttgggtgaag
     8461 aaacttcgcg cagagcaatc tgcagttttc tttcagtcac tggttttgtg agggcgattg
     8521 cgtttccgta ttttacacaa tagtcaatat tacagctttg ctcaaattgc atccccacac
     8581 cgataaacgg tttgccggat tcacaggcag tttggacggt cccttccccg ccgtggataa
     8641 aagaaaaatc aatgaattca tgcaaaagat gactagacag atattctgtt attaaaagat
     8701 cttctgagtc agattgaatg tcatcaagca gatagtcaat aggtgcaata accgctagat
     8761 ccggttgttt ttgtaagaag aacaaaattt tgcgtaacat acgcggattg cctgaactac
     8821 ccattgcgaa atagataata cgttttcttt cttgttgctt tcgctgtata aaagttagca
     8881 cttcttcagg caaggaagta ctgagcttag caaaaattgg accaacgatt cgataatttt
     8941 ccggcagctg ttcttcttcc aaaaatagtt taggcgttgt gatgagattt agatcagcat
     9001 ccatcatttg taaacttgtt tttggtaaag gaagcatttc tttttgtgca gcgtgtttga
     9061 aggcattggg gatccatcgg gtatttacta tacgcttttt tgtccagtgc caaaggggat
     9121 tgcttaaagg gccgaagaaa cgtaactcgt ggggcatcag cgcatgctct gattgaaaaa
     9181 aaggttcact caaagcatag ggtttaacat atactaaagg aatcttttta ttgcgggccg
     9241 ataaaaaaat tgtcacattc gaacctgtca caatcagtcg aggctcaaac ttagcaatca
     9301 gactctgttc actagtgata cgagaggcaa ccatttcttt ggtaaaaggg tttttaagtg
     9361 tacgcaactg gtcaaatttt atgatttgtt cttcttgttt tttcgtaagg atcggcgcta
     9421 gtaaatgata ctcaaagcca ctttctgtga tcaaaggagc aaaagtttta gaaaagccaa
     9481 agaatacaca gtaataacgg tctttcatag ctttggcaac ctcaatcatt cgtgttgtct
     9541 cagctaaatt gaatactgca ggagcaaaaa gaattgtctt catgaaatcc tccttattgg
     9601 aatgagtcta tcccttcgtt tcttactagt atactaaaaa acacagcgtt cgagtagcct
     9661 atgtatattt tgaatattaa actgtctctc gctgttttca caacttctaa atttaaattt
     9721 tgtaatcaag ttgaaactcc aattttcaaa aaaacacgca acaatttttt gatcgattta
     9781 tatgttgtgc cagataaata cgagaagttg tgtcattaaa aataaacatg agaacataaa
     9841 aaaataaaat aa
//
LOCUS       DAJSVF010000099         9809 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSVF010000099 DAJSVF010000000
VERSION     DAJSVF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.102"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(33..2153)
                     /locus_tag="ON550_002410"
     CDS             complement(33..2153)
                     /locus_tag="ON550_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3955443.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="ON550_002411"
     CDS             2383..3561
                     /locus_tag="ON550_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR3955444.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="ON550_002412"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="ON550_002412"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR3955445.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="ON550_002413"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="ON550_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR3955446.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="ON550_002414"
     CDS             complement(4914..6167)
                     /locus_tag="ON550_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3955447.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="ON550_002415"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="ON550_002415"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR3955448.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="ON550_002416"
     CDS             7745..9091
                     /locus_tag="ON550_002416"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR3955449.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="ON550_002417"
     CDS             complement(9399..9785)
                     /locus_tag="ON550_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3955450.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3127 a         1782 c         2009 g         2891 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttttctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttattttta
//
LOCUS       DAJSVF010000100         9788 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSVF010000100 DAJSVF010000000
VERSION     DAJSVF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.103"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            9..329
                     /locus_tag="ON550_002418"
     CDS             9..329
                     /locus_tag="ON550_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HCR3955451.1"
                     /translation="MRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLALDEDDLYD
                     QKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSVEIIEFVA
                     DKEE"
     gene            443..682
                     /locus_tag="ON550_002419"
     CDS             443..682
                     /locus_tag="ON550_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955452.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            749..988
                     /locus_tag="ON550_002420"
     CDS             749..988
                     /locus_tag="ON550_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955453.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            1115..1804
                     /locus_tag="ON550_002421"
     CDS             1115..1804
                     /locus_tag="ON550_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCR3955454.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1810..2976
                     /locus_tag="ON550_002422"
     CDS             1810..2976
                     /locus_tag="ON550_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3955455.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            3058..3729
                     /locus_tag="ON550_002423"
     CDS             3058..3729
                     /locus_tag="ON550_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3955456.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3782..5758
                     /locus_tag="ON550_002424"
     CDS             3782..5758
                     /locus_tag="ON550_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3955457.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5771..6523
                     /locus_tag="ON550_002425"
     CDS             5771..6523
                     /locus_tag="ON550_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3955458.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            6539..7300
                     /locus_tag="ON550_002426"
     CDS             6539..7300
                     /locus_tag="ON550_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955459.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7313..7573
                     /locus_tag="ON550_002427"
     CDS             7313..7573
                     /locus_tag="ON550_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955460.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            7570..9660
                     /locus_tag="ON550_002428"
     CDS             7570..9660
                     /locus_tag="ON550_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3955461.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
BASE COUNT         3505 a         1516 c         1973 g         2794 t
ORIGIN      
        1 atccgaatat acgattgggc acacatacga ttaaggtttc tttccaacgt tgggattaca
       61 aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt ttagcattag
      121 acgaggatga tttatacgat caaaaattga ctgataatcc aattggattc ggcttgctac
      181 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tacggatgat aatggagacg
      241 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc gttgaaatca
      301 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
      361 cttagaagga gtcacgacga ataataaaaa ggtagtatta ccttaggcat tgagtaagga
      421 atttatttag gaggaaaatt agatggcgcg accagtttta gaggcgatgg atatggatac
      481 tttgacaaaa ggaaggtatg tacagctgtt agctcgtggt tattcaccta aagatgtatt
      541 caaatccctg tctaaaggaa cagatatgga aaaagaaaga ttacgaaaag aattcgatta
      601 ttggagaacc cataatggaa tcaaggattt gaaaccagca cggccagtaa ttaaacgaaa
      661 gaaaaagata acccaagact gaagtgtaga gcgtatgtga tcataggctt ttttattttt
      721 gcaaaaaaca agaaaggtag gaaaactcat gaattatgaa gaaatcgaaa acagaaaaaa
      781 agtgtcgaaa gaaatggaag aaaagttgct aaaaatgatg aaacaaaaac accttaaaag
      841 attatctgtc atgcaatata tcaatgatat gaagataaca ggaaaagaga aagcttgttt
      901 gttaggttca atgaaaaact ttgaacagtt gaggcgaact tatgtaaaaa caggttccaa
      961 ttgccaacta ttattagaag tttcttgacg attatgatat gaaaatgatc aattatgatg
     1021 tggcattgtt attgaataaa aagaatagta aaatttaaca tggtttatct tggttagatg
     1081 taggtaaatt aattcatata tagaaggtag atagatgaat aaaaacaata aaactttatt
     1141 taatccttac gtgggcgtaa tcattgtttt tcttcttttt agttattttg cttatcaagg
     1201 ttatcaaaag gacaaaatcg ctgaaaaagt agatggtgta acaaaaacaa tccatattca
     1261 ggaacaggct aaaacctatc aagataattt aaccagttta acggatattt ctaagagaca
     1321 actgattact aatcaaaaac aggcattaga aaaggatctc aaaggtgaaa ttgctggaaa
     1381 acttgttttt tcttctacag gatttattct ccctgttttt caaggagcga atagcgacac
     1441 gcttagttta ggagtagctt ctacttatta tctagatact gaaatgggaa atggaaatta
     1501 tattcttgct ggttataacg tagatacacc aagcgtttta ttatccgata tgactgattt
     1561 atctgaagga gaagaaattg atctaatcga tgctcattac aagtatcagt atgaagtctc
     1621 caaaagagga aaaatttctg attacagcca ggtagttcaa tctaaggaaa acaattcctt
     1681 tttatccttg ccaaaagcag gtaaaaaacc actactcaca ctgttagcct ttcaagcaaa
     1741 taatatgaaa aagtgcgatg tgatccagtg tgaattagtc aatgtagaaa aaatcaataa
     1801 ataagggaag tgagtcacat tcaagaactt gtagaaaaat tgatcaataa aatgagtgcc
     1861 atttttctgt tactgttggt atccattcta tatttaatat ggacggtggg gttaaatcct
     1921 cctacgatat tatttattta tgataatgct gcacagttag tgacgttttt cgtaggctgt
     1981 ttgtttcttt tgaatctgta caaggtaaag agaaacgatc tattcttcat tgcagcaggc
     2041 attctttttt accttttctt ttcttttttt agaagctacc gaggagatac tttctatggt
     2101 gatctgatcg ttccgacaat tattgctatt attttgattg taaaatggac gaaatttact
     2161 gtaattgatc gtagcttgta tcttgtagta ttctatgtct ttttactcgt aacggtttat
     2221 cgagtattta ctgagatacc agtaccagaa ggacaaagta tttggaatcc atcgaacaaa
     2281 ttatcagata tttggatcaa tacaaatacg ataggtagtt ctttaatgac attagccctt
     2341 cttatcacag gatttgctag ttcatttgga aaatggtaca tgcgattgct atcattacca
     2401 gcgttgattc ttgctggggc aacgatttgg gtttgccaat cgaaagcagc attatatgcg
     2461 cttgttatct tcattatctt agaaatttta cctaagaagg tttttcgaaa tacacacttg
     2521 tccttttgga tgtatgcagc agtttcggtt ctagctttac cgatttctta ttttgctgca
     2581 atgtcagaca aagttcactt atttacagga cgagaagaaa tttggcgcaa attctatgaa
     2641 acattaggcg aaaaaacagg acaaatattt gtagggatga aacctttctt tttccataga
     2701 ggaaatcaga ttctaggtaa tcataatacg tatcacgcac tcgtcaatca ctatggattg
     2761 attggttttg ttctatcgat tattcttttg cttagtttct tatggttgct cgtaagaaaa
     2821 cgtgagctga caaatggaca actaacattc ttgtgggctt ttttagcgat catgtgtcaa
     2881 tcgttcatgg aagaaacatt aacgacaatc agttgggtgc caattgtatt cttaatgtta
     2941 gcaatggcta cacaccaata taaaaccacc agataaatga aatagaatag taataagaga
     3001 aaacactttt tcgttgtgaa aaagtgtttt ttgttttgtc tgaaaggagg cagatggatg
     3061 aaaaaatggt tatttggttt tttaggagtc gctttaatcg tcgtttgcag tgtgtttggc
     3121 tatgttagct accaaaaaca tgaaggagag gtgtttaagc aaaatataga aaaaaagatg
     3181 cccgtagatc aaatcaatgc acacgcaaag agttataagg aggatgcaac gaacgtcaat
     3241 aatgatatgt ctttggggca aatgttgtcc atacaaaaag aagcaatcaa aatgggcgtc
     3301 aataaacaag tatttgcaca aattcagatt cctgcacttg gtttagcatt gccaattttt
     3361 aaaggggcaa atcaatacac tttgagtctt ggagcggcta cttattttta tgaagatgcc
     3421 aaaatgggca aaggaaacta tgtcctagca ggtcataaca tggaaatgcc aggtgtttta
     3481 ttttctgata ttcaaaagtt gtcattaggg gaggtgatgg acttagttag taacgatggc
     3541 gtctatcgtt acaaggtaac gcgtaaattt atcgtacctg agtactttaa attaatcgat
     3601 ggcgttcctg aagagaactc tttcttgtct ttaccaaaga aaggagagaa acccttatta
     3661 acgttgttta cttgtgttta tacgtcacaa ggaaaagaaa gatacgtggt acagggagag
     3721 ctacaataac aagaatttaa tcaacgcgag tcgttgaaat atgaaatgag gagagtaaaa
     3781 aatggcaata aatcggtatg taaaaaaagc tggaatgatt gttggaatgt taggaatcat
     3841 tgcaggctgc tttcaggggt atcgagctta tgcagaagat gtcacgcaaa aaacgccacc
     3901 agaaagggtc aatatcacgg ttcataaatt aatgtacgat caaagtacgc aattgaacgt
     3961 tgatattgac ggaatcaaaa atgatggtta tacgcacgat caatatccta caggtgtaac
     4021 gaaatacaac aaggcagatt atggtgatgt tgagtttacg ttaggaaata ttacggatca
     4081 agtattaccg actgaagatt ctgatctaac caacgcgaaa gtggacgaaa tcgttaaaga
     4141 tgtagaagac aagggatcta acagtgagta cgttaagaat gcaaccaata aaattacttc
     4201 agctgtagat gaaaatggtg agatcacttt tgctgatcaa ccagcctatg tcaattccaa
     4261 agggaatgta tatgtggttt atgaaagtaa atccgctgct ggattagtca cacaaaaagc
     4321 aaaaccaatg gttgtgattg cgccaatgac agacaataca ggatcaggat ttttaaaaga
     4381 tattcatttg tatccaaaga acattgtttc taagttgagt tttgaattaa cgaaatttgg
     4441 tgacgatggc acagctcaaa gcaaacaaac acctttaaaa ggggcaaaat ttgagttata
     4501 caagggcgaa cctggaaaag gaacaaaatt aggagagctt gtatcggatg atcaaggaaa
     4561 gctgactgca actgatttga cgttaggaaa atactacttt gtggaagttc cttcagaagt
     4621 tgtagtggga tcagatcaag aaccaacagc agaccaatat ttgttaggcg cagacgctcg
     4681 caacgatgcg cacaataagt taacctttga aattacgaat gacggcgtga caagtgattt
     4741 aaaagcttcc tacgtgaact ataaagcacc agtgatcgat aaaacagtga caaatggaac
     4801 aggagaagaa cattctttcc aaattggtga cgcagtgaat tatcaaggta caattcatat
     4861 ccctacagat atcgcaggtg gaacagatgg gattacagta aatggtgtaa aaagtgaaac
     4921 ttctccttac agtgtattta aatggggcga cacagcaggt aaaggacttt cttatgttgc
     4981 tgaaaaagca gatattaaag taacaaataa tgatggttca gtcgtattaa aagaaaatac
     5041 tgactataaa atcgaaaatt ctgatagtgg ttttgtgatc gattttatcg taaataatgg
     5101 acaagtaagt gatacagttg cgaatttaca tggtcaagat ctaaaaatga cttataacat
     5161 gtttgtgaat gatagtgctg aaatcgcaaa tccattaaca aatagcgtgg atttcgttta
     5221 taacaataat ccatttaatc aggaagaaca ccacgaaaaa acgaaagctg acgttgtgac
     5281 ttatggcgca aaattcttaa aagtcgatag cggcctattt ggaacaggta tcaaagcaac
     5341 accgcttgaa ggcgcagaat ttgcagctaa gaatgcagaa ggtaagtact acggtggact
     5401 tactgataca gataaagatg gtgtcaaaga agcagtttgg gtagacgatg tagcaaatgc
     5461 agcaatccta aaatcagata aagagggtca ttttgaaatc actggtttaa ctgaaggaga
     5521 ttactcactg gaagaaacaa aagctcctga aaactatcaa aaattaacaa aagagatctc
     5581 tttcaaagta gacaaagatt catacaaaga agaaaaccga atcacgatca aaaataatca
     5641 aaaagcttcc gtaccaatga ctggtagcaa cggtttccaa tcttacgtat tgatctcttg
     5701 cttgttgctt ggggcaggcg caatttctgc agttgtttac tttaagaaga aagcctagaa
     5761 ggatcagcta atgattatca gacaccctaa gaaaaagcga atcatgggta aatggatcat
     5821 tgctttctgg ttactttcag ctgttggtgt cttacttttg atgcctgctg aagcgagcgt
     5881 ggcgaaatac caacaaaatc agcagattgc cgcaatcgat cgaacaggaa cggcagcaga
     5941 aacggattct tctttggatg ttgctaagat tgagttaggc gatcctgttg gtattcttac
     6001 aattccaagt atttctttaa aactaccaat ttatgacgga acttccgaca aaattttaga
     6061 gaatggtgta gggattacgg aaggaactgg cgatattaca ggaggaaacg gtaaaaatcc
     6121 tttaatcgca gggcacagtg gactttataa agataatctt ttcgatgatt tgccttcagt
     6181 taaaaaggga gaaaagtttt atatcaaagt tgatggtgag cagcacgctt atcaaatcga
     6241 tcggattgag gaagtgcaaa aagacgaact acaaagaaac tttgtcactt acttagaacc
     6301 aaatcccaat gaagatcgag taacgctaat gacatgtacg cctaagggga tcaacacaca
     6361 ccgtttccta gtgtatggga agcgagtaac ctttacaaaa tcagaattaa aagatgaaga
     6421 aaataagaaa caaaaattat cttggaaatg gcttttagga agtactgttt ttctttcagt
     6481 catgatcatt ggttcgctat ttgtttataa aaagaaaaaa taaaggaggg taaccaacat
     6541 gagaaataga aaatggctat ttgtttgtgc agccgctctt ttttctttca ctgcctacac
     6601 acagttgacg tttgcagaca gacaagaaag cacggatagc acagaacaaa caatccaaac
     6661 gtcagatagc agcacgattc aaagcagcga tgaaaccgtc gattcgtcaa cagttgattc
     6721 ttcagaagaa cctaaaattg ctgaagacga tcaaatagtc aaacagacca ttcacgttca
     6781 aaaaattatt tcttccgaac aattagacaa ccaggggcaa aagcttgaaa aacaagaagg
     6841 agtaaatggc gcgaagtttt ctgtttatga tgtatcggac attttacaaa agatggatgt
     6901 taaggatctc actactgacc aaattgaatc ccaattaaaa gatcgagtga aaaaactatc
     6961 cagtgatcaa ctgaagctaa tttctaatgg agaaacaaag acaattgatc aacaaacagg
     7021 tgtctttgaa ttctcggtag aagtacaggc aaatcagaaa caagcttatt atattgtcaa
     7081 tgaatcaagt cctgaaaaca tttctaatag cgaggatatt ttgctattaa cacctgtttc
     7141 tgataaaaat ggtgaattct taaaggatgt gtggatttat cctaaatcag aagcttcgca
     7201 acctaaagaa gaagtaaaaa aaatcgtatc tactggcgta aagaaaaact tctttgagaa
     7261 ttgttgggat tttgttactc acttgttttc tagagattaa ggaagaaata aaatgaaact
     7321 gatcaaatta ttattattca atttttcttg tttaaccgca gtctttttgt actgcggttt
     7381 attattttct aaactatcta ttttgccaag ctaccaacta tacctaatga ggattttaaa
     7441 cataccagct gcttccatgc cgctagatat tatggaaaag ctcattagga atccattatt
     7501 tactacgatc aatttgatcg ttattgtcgg aatttcacta gtcatttata aaaaagaggg
     7561 gatgaaggga tgaattttaa acactcgtgg acaagacggt taaaatatgt ttttcccata
     7621 atgatgatgt taggtacatt gtttgggtta aaaacagcca atgtctttgc tgaaaaagtt
     7681 attgtcgatc cgtcaaatcc aatccagaac gtaaagaata attttattat tccaaaagat
     7741 gttcctaatg caaaaacaaa tattactgta aacggagcgt caaaagttta caaatatcat
     7801 gttgatgcca acaaaggtgg ttttacagac gactacgtag gattagtgga gggaaacaaa
     7861 aatatacgct atccttcttt ccaaaaagaa tacggtgaaa caaaccttgt tttagaaggc
     7921 gtaacaacca acaccaaagt caatattgac tatggaaaaa tcggtacgta taacgggaaa
     7981 aaagtcaata tcaagctagt tctatctaat attcacttat actcggatac gttgccgtgg
     8041 aatctactag ataacaacta tacaaaaact cattttcgag acgatggcta taaaaatacg
     8101 aatggtgcaa tgagtaaatc taaaaaaaga acagtacttt ggatctcaga caatcttttc
     8161 tcaggaattg tttaccattc aacacaaatg aatgtgcagc tagtagccac atatgaggac
     8221 ggtagccccg ttcaattttc aggagataca tttatcagtt ttaactcttt gaaccctgca
     8281 ggaggaaaaa gcacggatct taaaggagaa tatgcccact atgacaaaat gaatacgaca
     8341 gattggtatg tcagaaaaga tacggttctt tcagaattta aatctttcta caacaatctg
     8401 aacgtggtag gtggccaccc aggaggaagc tctaagctga cacaagcgga taatgatttc
     8461 aataatttgc atgataaatt aggagatccg aagtttggtc aaggtactgt gtcctttaaa
     8521 atatctgaag caaatccgac gtttgtgatt ggttcttcta atgttcaaac ctggtttact
     8581 ttatcttctg cgacaatctt tagtgttgtt cctgatcaac ctgaaaaaac aggggttgat
     8641 aaaaatggaa ataacgtgaa cgataaaatg ttgcaagtag gagacacgat tcaatatcga
     8701 atcaaacaga aggtgaatcg attaggagtg gatttactag cggtttacga tcgatttgag
     8761 ttaatcgata atttaccaaa agaagtaaat tacgtcgatg cacacgttga aagtggtaca
     8821 aataaaaaat tcgatgtatc tggtgaagtg acctatgaca aaacaaaaca ccaggtaaaa
     8881 tatgcagcga aagctgatac attaaaaaat cgaatgaagt ataacggcga aacttacgaa
     8941 ttagtgatca atgtaaaagt caatgaattg gccaatcaaa atagtgtagc caaaaatcag
     9001 ggaacgtcga tcattaataa agtagaaaag gatacaaata tcattaccgt ttatttccct
     9061 aaaattccag tgaaagaagt acaacaaaat ggaaaagatg taaacggacg aaatgacggt
     9121 aagaagggta cgccgacagc tcctttaaac gcaggatctg aagtacaata tctagtcaca
     9181 caaaaatggc acacaaaagg cgtagacgct gcgtcagatc actacaaaca atttagtatt
     9241 caagatccaa ttgaagctcg tttgacatat aaagaaggtt ctgcgcaagt aatagataag
     9301 agtaaaggaa aagatattac ttctgaaggc acactaacct atgatagcaa ttcaagaacg
     9361 ctgaagtggg aagcttccgc tgacttctta aataacaatc ttttagatgg acgagaaatt
     9421 cagctgatat ttacagctaa aactccatta caatcagaaa aaaatattga taaccaagcc
     9481 gtagtagcag tagataatgt ttctaataaa acaaatgttg tgacaattgg ggttgatcct
     9541 aacttgccac aagttattgt tcctaaaaca ggttctacac atttagtaac gattttagcg
     9601 gtcagcttac tattacttgt gttagcaact tttggctacg cagttctgaa gttcaattaa
     9661 acaaaaaaac aaggctgaaa atcagccttg tttttctatt tgaaaatggg attggcacta
     9721 ataaatacgt tgtctgaaga aagtgatccg tctgcagaaa cttcataagc atgggataaa
     9781 ccaacgat
//
LOCUS       DAJSVF010000101         9715 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSVF010000101 DAJSVF010000000
VERSION     DAJSVF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.104"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            283..522
                     /locus_tag="ON550_002429"
     CDS             283..522
                     /locus_tag="ON550_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955462.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ON550_002430"
     CDS             complement(884..1144)
                     /locus_tag="ON550_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955463.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ON550_002431"
     CDS             complement(1329..1826)
                     /locus_tag="ON550_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR3955464.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ON550_002432"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ON550_002432"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR3955465.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ON550_002433"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ON550_002433"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR3955466.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="ON550_002434"
     CDS             complement(4548..5210)
                     /locus_tag="ON550_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3955467.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="ON550_002435"
     CDS             complement(5220..6035)
                     /locus_tag="ON550_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3955468.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="ON550_002436"
     CDS             complement(6297..6740)
                     /locus_tag="ON550_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR3955469.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="ON550_002437"
     CDS             complement(6874..7212)
                     /locus_tag="ON550_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3955470.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="ON550_002438"
     CDS             complement(7200..7577)
                     /locus_tag="ON550_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3955471.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="ON550_002439"
     CDS             7801..8994
                     /locus_tag="ON550_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3955472.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="ON550_002440"
     CDS             complement(9160..9582)
                     /locus_tag="ON550_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955473.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2896 a         1938 c         1592 g         3289 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgat
//
LOCUS       DAJSVF010000102         9658 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSVF010000102 DAJSVF010000000
VERSION     DAJSVF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.105"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(372..1682)
                     /locus_tag="ON550_002441"
     CDS             complement(372..1682)
                     /locus_tag="ON550_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3955474.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIRD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1722..2990)
                     /locus_tag="ON550_002442"
     CDS             complement(1722..2990)
                     /locus_tag="ON550_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3955475.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     LAKNITGPYELDPGFPMLTASDKPASTLQCTGYGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNAGRILPNADWCDTKCRPSMCRSGRRIYAGTHWLPIVYNRWSHPISFSTRKDLYDG
                     KLVTSILLICIRNHFASRLPTESFKNCIYW"
     gene            complement(2994..3404)
                     /locus_tag="ON550_002443"
     CDS             complement(2994..3404)
                     /locus_tag="ON550_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR3955476.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHPTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(3401..4324)
                     /locus_tag="ON550_002444"
     CDS             complement(3401..4324)
                     /locus_tag="ON550_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR3955477.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKLLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTIKFSEHNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(4321..5199)
                     /locus_tag="ON550_002445"
     CDS             complement(4321..5199)
                     /locus_tag="ON550_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR3955478.1"
                     /translation="MQLNDFLGAPSSRRTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVAALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(5186..6523)
                     /locus_tag="ON550_002446"
     CDS             complement(5186..6523)
                     /locus_tag="ON550_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3955479.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETGRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKTAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSKQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(6526..7629)
                     /locus_tag="ON550_002447"
     CDS             complement(6526..7629)
                     /locus_tag="ON550_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3955480.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(7703..8956)
                     /locus_tag="ON550_002448"
     CDS             complement(7703..8956)
                     /locus_tag="ON550_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3955481.1"
                     /translation="MASDELGDIVTLQIAMLKNSSVRNALKSGVFWDVSEYLKDYKNL
                     SQISEDRIEAASIDGALYGVPIQKDYARAGLVIRKDWLDNLGLDVPTTMDELYEVAKQ
                     FTENDPDGNGVDDTVGFGDRAPSDIRYTSFKLFTSYFGAPNGWAVDDNGKFTPEFDTE
                     EYMKALNFSRDLFENGYLAQDFAVTQKTDQQEQFAQGRTGIYTGMVDIKNLKTMGQGI
                     QDDMELVPVNKISDGEGGGYHVWSEGNGIGGLLAFPKSQVKDEAQLKKLLQFVDDLLE
                     EDVYKLMTGGIEGTHYELNDDGSIEFIDMDLWQQEVQPLSSSRPSEVTFSFKDTDPEK
                     EFSNQLIKENEEFAVMDPSVPLDSPTNNEVGSEIEKIVIDATVQYIMGQLDEDGFKKA
                     VEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(8975..9211)
                     /locus_tag="ON550_002449"
     CDS             complement(8975..9211)
                     /locus_tag="ON550_002449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955482.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASK"
     gene            complement(9177..>9658)
                     /locus_tag="ON550_002450"
     CDS             complement(9177..>9658)
                     /locus_tag="ON550_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955483.1"
                     /translation="NYRETLLMSWIYGAIGIVLLVDLSFIHNWYLRIALYIAAFIYVL
                     SALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGIYLMIIQTMPGVLT
                     FIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEKIYEKSNARKIRS"
BASE COUNT         2748 a         2110 c         1618 g         3182 t
ORIGIN      
        1 agagtgtttc ccgattcatt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttgg agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt ttaacgactt agttatctta tcacgtctaa
      241 aaacatgtgt caactctttt ttggaatttt cataaaatgt ttcaatgaag cgcgacaacg
      301 ttgtttaata tatcattaaa aaaaataaag gtcaagctaa aatattattt aagcttgacc
      361 ttattataat ttcaacgatt gtcaaaagta ttgtttgcag ctgtattgcc agtttctata
      421 gataaatcac agcttttgaa acataagttt tgtactgctt cgattcgccc gaattctttt
      481 gcagttattt ggcaatgaga aaagcagaca tcttctatag gcttttcagg aaatgctact
      541 aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg
      601 atgtgtatgt ctttgacttt cgtcatttgc tttgattcag gtatttcttc agccactgct
      661 tgccaacttt ctggcatttg agcaaggtca ttgctatttt ttctattgta ttcattatgc
      721 cagtccatta tccaagaaaa aggaaattgt acatcaatca tattcagatt ttctaccctg
      781 atattttcga tcactccccc tctttctttt gaagatttca ttcgaatacc acagtctgac
      841 tgaaaaaaat cgatatcgcg aatatatacg tcactaactc cagcactaac ttcgctacca
      901 atagttacac cgtatcctga acggatttta catctagcaa cctcgattct tgatgatttt
      961 cgattgactc ttgcaccatt gccatccctg ccagacttaa tagcaataca atcatcacca
     1021 caggccaaat cacattcata gacacgcaca tttgttgaag aatcaatatc aatcccatct
     1081 gtacttggtc catcattgtt gcggatgatt actttttcta ctaatacatc gttagaataa
     1141 gtgatttgca agttccaaaa acctgatttc tgaaaaatca aatcacgaac aacaacatgt
     1201 tctgcatgat acagtaaaca agcgcgtgga cgtttaatag cataatccgc aatccaacga
     1261 agtccctttt gatcgtattc agctcttgtc ccgctttttt gatcttttcc ccaatacagc
     1321 tcccaccaat ggtctccttg accgtcgatt attccttttc cttctataag aatatctttc
     1381 gccgagagaa cattcaaaat cgctgctggc catttcattt ctacgccggc tactcgtgta
     1441 tcgatttctg gaaatgcttc gatatctgtt ctccccttga ggactgcacc ttcttcaaag
     1501 ataagatgac tcttatcttt taaaaaaagg gctccgacta aataaatgcc ttttggaata
     1561 aatacaggta agtgttcttt cgcaccttga tcgattgctt tttgcagtat tattgtatca
     1621 tcttgttgag tgtcgtttgt tttaaagttc aaagcattga taatcccttt ttttgtccgc
     1681 aatacgtttc tccctatctt tcattgattt ttttcaattc ttcaccaata aatacagttt
     1741 ttgaacgatt cagtgggtaa cctactagca aaatgattgc ggatacaaat aagcagaatg
     1801 ctggtaacaa gtttgccatc gtataaatcc tttcttgtac taaaactgat tggatggctc
     1861 cacctgttgt agacgattgg taaccaatga gtgccagcat aaatccgcct accgctcctg
     1921 cacatgcttg gtctacattt tgtatcacac caatcagcat ttggcaaaat gcgtccggca
     1981 ttccagttct tctttagctc tccttcaaat gtatccataa actgatgatc tttttcttgt
     2041 tgtacctctt catttgtctc aatcattatt tcttccatcg gagttgtctg gctatcttta
     2101 agtcttaacc aatcgtcgga cgtccaatac acttcttgaa tagcggtttc tcttcccaaa
     2161 atagccgcac cgtttaatgg tctggtacaa agatatgtca tgtaccatct tcctgacggg
     2221 gtttgtacta agctcccata tcctgtacat tgcaatgtag aagcaggttt gtcactagct
     2281 gtcagcatag gaaaaccagg atcaagctcg taaggaccag taatattctt tgctaggcaa
     2341 actgtaactg aatgtcctct tcctgtaccg ccttctgccg tcatcaaata ataatacccg
     2401 ttacgaaaat acaaatgtgg tgcctccgtt ttcgctaatt ctgtacctgc aaatatttta
     2461 tagatgggac catcaagctt cttcttctct gcatcatatt cttgtaatac gataccgctg
     2521 gatttattcg gtgtgtccag acgataatcc catagttcat tgacaagcca tttttttccc
     2581 gtttcaagat catggaacaa agaaggatca aagccgttac tatttaaata aattggttcg
     2641 gaccaaggac ctgtgatctc agatgcagtg atcaaataat tgtggacatc tttaaacgga
     2701 cggcctgcac ttttgacatc tgtgtaaaca agataaaaca atccatcagc ataactgatc
     2761 tgtggcgccc atatacttcc atttgtagga tttccttgca aagatacttg agtggtcaaa
     2821 atatccgttg tatgtgacca gttgactaaa tttttcgact catatactcg gattcctgga
     2881 agccattcaa aagaggagac aataatgtaa taccgctctt tcactcttaa tatattagga
     2941 tctggattga atcctcttaa aataggattt ttcactttga tagtctgcat gattcaggct
     3001 ccttttctca ttcttactcg atataaactg aatgccccag ctgcgaaata aaaaaatgca
     3061 aaaggaaacg caataaactg agtgccaatc aacaatatga atcctgttat aaaatacaaa
     3121 ccagtggcag ttgttaagta ttttccttta gcagtcaaaa acaatgctac tgctaaaaaa
     3181 gcaagtgaaa taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat
     3241 gtattttcta aagacaaagt tggatgatct acagcaagcg actgataact ttttttaaac
     3301 tctgacgcag acatattact taaagtaact gctaacccgc caaagaaaat caaagataga
     3361 ctgccaccga ccattcctag caatagttcc cattttcttt tcatttctga accccctgct
     3421 tttctaaatg tatccattca ggtcttttga tcgatgtttc ataattgtta ttatgttcag
     3481 agaatttgat agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa
     3541 catgcccacc tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc
     3601 ctaaatagac gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc
     3661 ctgttttttg atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta
     3721 agctatggat atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg
     3781 ttccttcaat ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg
     3841 gtgtacaaaa aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat
     3901 cctgataacc agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag
     3961 caacagcctg tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta
     4021 atacgacatt cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac
     4081 cgtcaagata ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca
     4141 caagtttaaa attggataat ctgctttcta tatattcatc atatcttcct tcttttacta
     4201 tcaagagctt tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag
     4261 tttcaaaatc cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc
     4321 tcataaatgc tcccttatga attgtttcca aatgtttctc atttcttgtg tttccatatg
     4381 accgcctgtt aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt
     4441 gtccagccct tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc
     4501 aactaagcta agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc
     4561 tgtcaataga tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa
     4621 aagtgtttcg atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa
     4681 tgctgccacc caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg
     4741 agtatctgtg tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg
     4801 cattccccat aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat
     4861 acctccgcgt tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt
     4921 cagtacttct gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt
     4981 cccgactgaa aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc
     5041 tttttctgga taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata
     5101 gtattcaagc ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtacg
     5161 tctgctagat ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa
     5221 ccagttcatc catattttct gattgatttg attggaattt ctcaatactg atttctttcg
     5281 tattttctaa tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa
     5341 ccttacctgc aaagccaatg taaaatcctt gcttgctcat ttcctctatc cctgtcatca
     5401 tagcagggac accgggaact gcatcttggg ccattgaaac atcaatattg tcaaatgtga
     5461 tttcttgtat aaactgttct gctaatccat aaataaatcc tgcagaagca tggacattcc
     5521 ttgccgtgat attcgaaaaa tggatccttc tgaatgctgg tgtccgctca tctatcggat
     5581 aagccgtttt ttcccatacg tacggttctt tccctcgcgg accacagaaa taataaagat
     5641 tcagtataaa tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa
     5701 cgatcccgcc tcgacctcgt ctagatttca atcgtattcc tcgaccagtt tcttggaaga
     5761 tacaatttga aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac
     5821 cgccatgtcc atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt
     5881 cgtacgtatc ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc
     5941 aattactaat acgtacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat
     6001 ccgctgggtt caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga
     6061 tcgtccaact tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat
     6121 ggaaactcat caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat
     6181 gccaccattt ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta
     6241 cattttgagc atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat
     6301 gaacaacagg ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat
     6361 gaagttcgac attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa
     6421 ctacggtccc gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat
     6481 tcaattcatc aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac
     6541 ttcaatcaat gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc
     6601 actgatgtag taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg
     6661 acagttttta ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa
     6721 tccttgatgt gatttcttca cttgtggaag ccattcttct ttgttcagca ggcccaaacg
     6781 tattccttta gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc
     6841 tttccgtttt gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg
     6901 aagtaatgca gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc
     6961 atgaagtaat tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca
     7021 aaaatgatgg gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca
     7081 tgcatgaatc agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg
     7141 tctcaaaaca tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa
     7201 aggagaaccc atatataagc catccaacca aatttgataa ggataaattt ctttatgcca
     7261 aaaagctcct tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc
     7321 ttttttatat ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt
     7381 tacatggtcg atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac
     7441 gaaatagtcg atattgtctt ttatgaagtc aaaataaatt ttttcttggg ttcgttccca
     7501 aagtaattgg aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca
     7561 cttttcttca tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca
     7621 gttttccatt atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa
     7681 gtatttgtcc caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt
     7741 gtgtgattcg atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt
     7801 cgtcaagctg tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg
     7861 atcctacttc attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt
     7921 cttcattttc tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag
     7981 agaacgtcac ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat
     8041 ccatatcgat aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac
     8101 cgccagtcat caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt
     8161 tcttcaattg tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac
     8221 catttccttc tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag
     8281 gcactaattc catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca
     8341 ccattccagt gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt
     8401 gggtaactgc aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca
     8461 aagctttcat atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag
     8521 cccagccatt tggtgctcca aaataagaag tgaacagttt aaagctggta taacgaatat
     8581 cactaggcgc acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt
     8641 tttctgtaaa ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc
     8701 ctagattatc caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta
     8761 tcggtacgcc gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt
     8821 gacttaaatt tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta
     8881 acgcattgcg gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta
     8941 gttcatcaga agccaatgca gttgttaatc tttcttattt tgaagcatca ggaatccagt
     9001 tgaatgtcag ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg
     9061 gtgaagcatg atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg
     9121 aagaactgtt acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa
     9181 gacctaattt tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata
     9241 tttttgtttt cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata
     9301 gaatttgctg tccacgtgac tgcggaaaag aagagactac caccgataaa ggttaaaaca
     9361 cctggcattg tttggatgat catcagataa atccctacca tacttaatat caacactaat
     9421 gagtaatgaa gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct
     9481 ttccaatcat aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac
     9541 gcagcaatat acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt
     9601 aaaacaatcc cgatggcgcc atatatccag ctcatcagca aggtttcacg atagtttt
//
LOCUS       DAJSVF010000103         9646 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSVF010000103 DAJSVF010000000
VERSION     DAJSVF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.106"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            185..1183
                     /locus_tag="ON550_002451"
     CDS             185..1183
                     /locus_tag="ON550_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3955484.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ON550_002452"
     CDS             1295..1804
                     /locus_tag="ON550_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR3955485.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ON550_002453"
     CDS             complement(1834..2493)
                     /locus_tag="ON550_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR3955486.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ON550_002454"
     CDS             complement(2495..3259)
                     /locus_tag="ON550_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR3955487.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ON550_002455"
     CDS             complement(3267..3944)
                     /locus_tag="ON550_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR3955488.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ON550_002456"
     CDS             complement(3981..5366)
                     /locus_tag="ON550_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3955489.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ON550_002457"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ON550_002457"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON550_002458"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON550_002458"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR3955490.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ON550_002459"
     CDS             6305..7162
                     /locus_tag="ON550_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955491.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ON550_002460"
     CDS             complement(7243..7578)
                     /locus_tag="ON550_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955492.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ON550_002461"
     CDS             complement(7645..9015)
                     /locus_tag="ON550_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR3955493.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ON550_002462"
     CDS             complement(9093..9629)
                     /locus_tag="ON550_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3955494.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2823 a         1992 c         1619 g         3212 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctctt
//
LOCUS       DAJSVF010000104         9605 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSVF010000104 DAJSVF010000000
VERSION     DAJSVF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.107"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..268
                     /locus_tag="ON550_002463"
                     /pseudo
     CDS             1..268
                     /locus_tag="ON550_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            807..1007
                     /locus_tag="ON550_002464"
                     /pseudo
     CDS             807..1007
                     /locus_tag="ON550_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="ON550_002465"
     CDS             1494..1673
                     /locus_tag="ON550_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955495.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="ON550_002466"
     CDS             complement(1719..2324)
                     /locus_tag="ON550_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955496.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2383..2841
                     /locus_tag="ON550_002467"
                     /pseudo
     CDS             2383..2841
                     /locus_tag="ON550_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3006..3233
                     /locus_tag="ON550_002468"
                     /pseudo
     CDS             3006..3233
                     /locus_tag="ON550_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3359..4330
                     /locus_tag="ON550_002469"
     CDS             3359..4330
                     /locus_tag="ON550_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3955497.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4470..5783
                     /locus_tag="ON550_002470"
     CDS             4470..5783
                     /locus_tag="ON550_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3955498.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5826..6692
                     /locus_tag="ON550_002471"
     CDS             5826..6692
                     /locus_tag="ON550_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3955499.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6689..7537
                     /locus_tag="ON550_002472"
     CDS             6689..7537
                     /locus_tag="ON550_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3955500.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7551..9023
                     /locus_tag="ON550_002473"
     CDS             7551..9023
                     /locus_tag="ON550_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3955501.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9040..>9605
                     /locus_tag="ON550_002474"
     CDS             9040..>9605
                     /locus_tag="ON550_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3955502.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIE"
BASE COUNT         3031 a         1631 c         1985 g         2958 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ctgctatcat agcattttta
     2401 aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa tgaaattaag
     2461 cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca gttaaataaa
     2521 ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg caaactttat
     2581 caaacttatt acgacggtat ggaggaacag ctcaacgcat taggattagt aactaatgct
     2641 atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa aacggaaggg
     2701 ttcgactgtt ccgaagaagt gttacgcaag caatttccat taattagtga tcacattaat
     2761 tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat actacgtccg
     2821 ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc attttcgcac
     2881 ggatgttcca gatactattt ttgaatgtat aaaattgaaa acacaagaat actaataaat
     2941 caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta tgttccatgt
     3001 accccaatct tgaaaaatta attaaagact tagataaagc cattgacgac ttagtcgtgg
     3061 tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt taggctgctg
     3121 gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa ttgacaaagt
     3181 atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa aattttttta
     3241 cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta atttggaatc
     3301 gtttccttct tttggtataa taatacctga aattcttttg agaggagttg ttcgtgaagt
     3361 gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta cggtatctag
     3421 agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg ctgcaattag
     3481 tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata gtagcaatac
     3541 aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg cctactatgt
     3601 agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga acggcagtgc
     3661 ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg gaattatagg
     3721 tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg tcagtataga
     3781 ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag gtgggcaaat
     3841 tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg ggggtgtttc
     3901 accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt gtgaggaaaa
     3961 taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttag aggaacaatt
     4021 gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca atgatttcat
     4081 ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta aggattttca
     4141 aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac tatcaacgat
     4201 tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg cgatgatttt
     4261 aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg gagagacgac
     4321 taaagagtaa aaaaccggtt tataacgatt tttttgagat aggttgacag aaagtgattt
     4381 caatgatatc ttaaaaccaa ttaattggaa acgttaccaa ttaattgaaa aataaaacgg
     4441 aaacgtttca aaaaatagga ggaacatcga tgaaaaagag aatgttagca attgcagcaa
     4501 ttttagcatt aacagctgga ggacttgcag gatgtggcag taaggagaaa agttcaggta
     4561 gcagtgatcc aggagaaatt aagatttggg ttcagttttc tgatgaaacg cctgaaggaa
     4621 aagcttgggc agagattgtg aacaatttta atgatgaata cgagggtgaa tataaagctg
     4681 ttactgagta tattcctcgt agcggaagcg gcggtggata tgaggataaa gtaaatgctg
     4741 caattacaac caataccctt cctgatgtta ttacgttaga tggaccgaat actgcagctt
     4801 atgctcattc aggagtcatt gctccattag atgattattt gaaagatgcg gacatggatg
     4861 atgtattaga cagcattatt cagcaaggaa cctacgatgg caaaatgtat gcgtttggtt
     4921 tctcagagtc cagtgtgggt gtctattaca ataagtcgct gttcaaagat gctggaatta
     4981 cagaagatca attgccgacg ttagaagatc cttggacttg gacggaattt caagcaattt
     5041 gccaaaagct gactgataaa tacaaaacgc ctgcaattga tatgcaaatg gcgtcgggag
     5101 acgaaatgct gacctacgca tatacaccat ttatatggtc gaatggcggc gatgtcgtta
     5161 atgctgatgg aaccaaagcc gaagactatt ttaacggtaa aaaatccgtt gaagcgttgg
     5221 aatttattca agaattggtt gaaaaaggtt acaccacaat cagtcctatt gaaaaaggat
     5281 ttgaaactgg caaatatgcc atgatgctga gcggttcttg gacaatggca gatttagagg
     5341 aaaactatca agagattgat tatggtattt tgccttaccc cgtttcagat gcaactgggg
     5401 agttagtttc accaaccgga agctggcagt tagcaatgac tgagaaaaca gagaaaaagg
     5461 atgcttctgc ggcctttatt aagtttgcaa ccagtacaga atctagtcgt attatgagtt
     5521 tagggaacag tgttctacct atcagaaatt caacagttga tttaattaaa gatgaagtgt
     5581 cggaaggaat gcaggtatta attaagcaaa accaactttc tggacatgcc cgcccagtgg
     5641 ttgtagctta tccgcaagta tctagagcat tccaacaagc agttcaagat attagctact
     5701 tcgaggaaaa cccagatgta caaaaagtag cagatagtcg cgctgctgaa atgcaacagg
     5761 caattgatac ttcactaaaa tagaagtttc taaagggggc caatcaagtg ttaaaaaaac
     5821 cgacaatgaa agacaatttg ataggttacg gctttctagc gccagctttg ctgctgctga
     5881 cgatttttct gatcataccc gtaatcatgg taatttatta tgcctttact gattattatt
     5941 tgttaacgcc agatgctaga caatttatcg gcttagaaaa ttttaagcga ctgttcgctg
     6001 atccaatttt tgtaaaaagt atttggaaca cggctaaatt tgttgtttgg attattccgg
     6061 tccaaatagg ggcagcttta ggaatggcac ttattatcaa caaaaatcgt aaaggaaata
     6121 tcttttttaa agtagccttt tttgcgccag tcgtgatgtc cttagtcgtt atttcgattt
     6181 tatggttgta cttgttaaat cctaatgaag gtctggttaa tacgctattt gcaaagattg
     6241 gaattgcttc gcagcctttt ttgactagtc ccaaacaagc aatgtatacg cttgttttcg
     6301 tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt tttaggggga atgcaaaata
     6361 ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt ttctaagttt cagcaatttc
     6421 gctatatcac catgccatta ttaaagccca cggcgatttt tattttactt accacattga
     6481 tttcggcctt taaactaatc attcagccaa tggtaatgac tcaaggtgga ccaatgaatt
     6541 ctactatgac aatggtttat tacatttacc aaactggatt tacagaccgt atggtaggct
     6601 actccagctc cattgcgtta atctttacca ctatgattgg tttaattaca attgctcaaa
     6661 gaaaattggt aaaggaggat gatccagcat gaccaagaaa tttggaccgc taatgatcgt
     6721 agaatattgt ttgctgttcc ttttagcggc attattcatt ttcccgatgc tgtggatggt
     6781 agtttcctct atgaagcctg aagcagatgt ctatcaaaat ttagcttcct tcaaagcttt
     6841 tttacctagt ttgaatccag ctaattggtt taaaacttat caagaggtta tttcccggtt
     6901 cagtgtggga acttatttat taaacagtat tttttatggt ttaacctttg cctgtgggtc
     6961 aatcattgta aactctttgg cgggttttgc ctttgcgaag gttaattttt caggcaagaa
     7021 aattatcttt ggagctttgt tagctctgct gattgtcccg atggagacgg ttttaattcc
     7081 gcaatttacc atcgttaatt cactaggctt agtgaatact cgcttggcag tgattttacc
     7141 agcgatggcc agcgcgttta atatctatct gtttagaaat ttctttatcg cgattccaga
     7201 agaaatcatt gaatcagcga aattagatgg tgcaaatatt gttaaaattt tctttcacat
     7261 catgctgcca atgtctaaac cagccatagc cacagtaggt gtcttatcct ttatctccag
     7321 ctggaatgat tatatttggc cgttaatggt cttaactgac agcagcaaat tttcaatgca
     7381 agtggcaatt actacgatca atactacaca gccagtttat atcaatcaag tgatggctgt
     7441 tttaaccatt gcaacgattc cactgattat tgtttatatc gttgcacaaa aatatattct
     7501 gcagggactt ggcggttctg gaacgggtat aaaataggag tgaatagtga atgttgcaaa
     7561 aacttttaaa ggccaatgag tttattgaga aaaatgctca aacagtcaat caagaatacc
     7621 ggaataaatt tcatttgatg gctccgattg gctggatgaa tgatccaaat ggttttgttt
     7681 attatcaaga ggagtatcac ttgttttatc aatattatcc ttacgacagc atctggggac
     7741 caatgcattg gggccatgct aaaagcaagg atctaattca ctgggagcat ttgccagtag
     7801 cactggcacc aggtgaaagc tatgacaagg aaggctgttt ttccggcagt gccatagaaa
     7861 aggatggcaa gctgtatttg atttatacag gacacaatgt tgtggaaggg caagttcgac
     7921 aagttcagtg tttggcagtt tcagaagatg gcattcactt tgaaaaatat gccggaaatc
     7981 caatcatcgc agaagatcac ttagcttctg ttgcaacgac cgaagatttc cgcgatccca
     8041 aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc tactaaaact gcagatgctc
     8101 gtggacaaat tgtgatgttc cattccaaag atttactaac ttgggagttt tattccgtat
     8161 tgctggaggg aactccagaa caaggcattg tatgggaatg cccagacttg tttgaactag
     8221 atggcaaaga agtcttgatt ctttctccta ttcaaatgac tccacaaggc aatgcttacc
     8281 aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga ttggcaaaca ggcaaatttc
     8341 tagtagagaa ttttcatgaa atcgatggcg gtttagattt ttatgcaccc cagacctgtg
     8401 aaaacgaagc aggccaaaga ataatgattg cttggatgca aatgtggcag cgtaaaatac
     8461 ctacgaatga tctgggccat ggttggtcag gttccatgac cttgcctaga gagctgaggg
     8521 tggctgataa tcaattgttg caacggccag tttcatcaat ttatcaagta attgatccgc
     8581 agaaaaggat tgaatgggcg aagactgaag agaatcgctt cttactgaag gacttagtat
     8641 atgaccagca ctatttacgc ttggaaatca atttaaaaca ggcaacgaaa ctagaacttc
     8701 actatgcgaa gggacccgac catacattac tgcttaacta tgatgttgaa cgggagtgct
     8761 ttactgcttc cagggaacac atgccgtatg atttatcagg agctgaaaag gaagtgttga
     8821 aaaagcgcag tgtgacgatt cctttggaaa ataattgtct aaccatcgaa attttccgtg
     8881 atacttcagc tattgagctt tttctaaatg atcgtctgac tatgagtaca actttttatg
     8941 agacagaaaa aggacaggat tttcttttgc tggcagacag tgaagtaacc ttttcattgg
     9001 aaagcggctt aattaatcta tagaagggag agctcagtga tgatttatgg ttcaatcgaa
     9061 gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga
     9121 gtgagctttc cgacaacgac acctgaggaa acaatacctt tagtcattga cttcttcaaa
     9181 caataccaag cagatttagc agggattggg attggctcat ttgggcctat cgatatccat
     9241 cgcgattccg ccacttatgg gtacattacc tcgaccccga aagtggcatg gcaaaatttt
     9301 gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc
     9361 aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt
     9421 tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa
     9481 gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt
     9541 gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc
     9601 gaaaa
//
LOCUS       DAJSVF010000105         9348 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSVF010000105 DAJSVF010000000
VERSION     DAJSVF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.108"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            589..969
                     /locus_tag="ON550_002475"
     CDS             589..969
                     /locus_tag="ON550_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR3955503.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            951..1865
                     /locus_tag="ON550_002476"
     CDS             951..1865
                     /locus_tag="ON550_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3955504.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            2105..2794
                     /locus_tag="ON550_002477"
     CDS             2105..2794
                     /locus_tag="ON550_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955505.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GFIFYLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2915..3001)
                     /locus_tag="ON550_002478"
     CDS             complement(2915..3001)
                     /locus_tag="ON550_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3955506.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            3645..4382
                     /locus_tag="ON550_002479"
     CDS             3645..4382
                     /locus_tag="ON550_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3955507.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            4410..4949
                     /locus_tag="ON550_002480"
     CDS             4410..4949
                     /locus_tag="ON550_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3955508.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            4965..5135
                     /locus_tag="ON550_002481"
     CDS             4965..5135
                     /locus_tag="ON550_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955509.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(5449..5910)
                     /locus_tag="ON550_002482"
     CDS             complement(5449..5910)
                     /locus_tag="ON550_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955510.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            6043..6417
                     /locus_tag="ON550_002483"
     CDS             6043..6417
                     /locus_tag="ON550_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCR3955511.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            6471..8597
                     /locus_tag="ON550_002484"
     CDS             6471..8597
                     /locus_tag="ON550_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955512.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLESIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            8594..9214
                     /locus_tag="ON550_002485"
     CDS             8594..9214
                     /locus_tag="ON550_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955513.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
BASE COUNT         3620 a         1415 c         1457 g         2856 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggtaagag tatgaaaaat tttatgatag acttatttat
       61 tctttgcaat tgcccaaaaa attaaaccgt caattacaaa agacttatga aactaaatca
      121 actgaagaat gggagagtta ttgctagatt tttggtagaa gtaacaaaaa gtggacatat
      181 tatcataatg actcgccgaa agtctgataa aaaattggct aaaaatattg ctgaatgttt
      241 aataaaacta tcagttttag ctaaatagtt agtagtttac acaaaaaagc atttatgttt
      301 tttgttgcaa agcaacaatt tcatcttcgg tgaaataagc gaaactttac aaaaagtggc
      361 tagcatttgc tagctttttt tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg
      421 agcttgcgcc cctttgacaa gcggttattt gcaccaaata cggaggtatt tggctagcaa
      481 atatagagct tgccaaaccc ttttagtgag ctttactcat tgtacctttt taggtacaat
      541 ggtattgcga acgatacatc cgtaggacgt actgaaaggg tgggacttat gagcgaacaa
      601 aatcaaaatt tggctagcaa ttattctaaa aaatatacct atcgatccga gccaaaacaa
      661 atcagttttc gagtgagcga atccgaattt gcaaagctga agcaatcggc tgaagctttg
      721 caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa acgcgcgtct ggtgacaccg
      781 aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt tagccaaagc tggagggaat
      841 atcaatcaaa ttgctaagtg gtgcaataca caccaacatg acgttgcttc tggtgacgct
      901 caacgtttat ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat atggcaacaa
      961 ttaaggtaag ccgaaccact tcttgtagtc gtgcaattaa ttatgcagaa ccacgagcaa
     1021 ccattaaaac aggaataaac tgtgacgtcg attatgccaa aagtgaaatg aaacaaattc
     1081 gcatgctgta tggaaaagac gaccgtgtac aggcacactt attgattcaa tcttttcgac
     1141 caggagaaat taccgctgaa aaagctaatc aactaggggc agagtatgca aaaaaaatag
     1201 cccccgatca ccaaatagct atttacacac atacagacaa agaccatatt cataatcaca
     1261 ttgtcattaa ctccgttaat ctggagactg gaaaaaaatt tcaagctcat ggtcaagcat
     1321 ttctaaacaa atgttatgac ctaaatgacg agatttgtct tgcacataac ttaccaatca
     1381 ctgagcgagg aaagaagcca gaaaaacgaa ccatgtctga aataaaatta aaagaaaaaa
     1441 atgaacctgt ttggaaagac gaaattcgga ttgcgatcga tcaaacaatg aaaaacaaaa
     1501 aaaccagaac ctatgaccaa ttttgtaatt ccctcaaaac attggggatt cattgcttca
     1561 atcgcgggaa aaatttcact tacgaattga tccacaaaaa gaaaaaagta cgatccaaca
     1621 gactaggaaa ggactacgaa aaggagacga ttttacatga gctggacaga cgagaacgaa
     1681 cctataacca acagcgaacc aacgagctac gaagagctcg agctagcatt cgcgaaactg
     1741 cagaagaagt acgaagagaa caacgaactc ttacgcctga acaaccggaa attagaccgg
     1801 ctcgaagaaa ccatgaacaa cacgttgaaa gaggaacaga ccaccaacaa gaattatcgc
     1861 tctaaaacgt taaaagacat agaaaacgaa ataaagaaac aacgtgcttt tctcgttcct
     1921 tatacgcaag aaaggataca aactacgatt gatttacaag ttaaaaaact ccatcaagag
     1981 ctattagacc agcgaaataa ggaacagaaa aaggtgctta ttcaacgatc attggatttt
     2041 cttgccccct tatgttgctt agtcctgaca ctgttcgcat tttggttagt atggggaggc
     2101 taaaatggca ttttatttac cctatctact catttttgtt agtatttcag gaagtatttg
     2161 gctcatctat aaaatttttc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa
     2221 gcgatttttc tttcttggct ttatatttta cttgataatc gtttcaagcg gcttattcgg
     2281 cgttttagag ggaaataagc gcgtttctag aagtatttta ttaggaaatg tcactcaaaa
     2341 gtatgaatca gcgcgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggaaga
     2401 gtttaccact tgttatgagg aaatgaacga tatttttgtg aatcaagaaa aacgtttgac
     2461 ggataaaaac atggaaaaac tcactcgtct ttatcaaaac ttgccagaaa aacagcaaaa
     2521 agaggttcaa gacaactatg agcgaaccaa aaaagacgtg caatatgtaa aagatacaaa
     2581 gatagaagaa gcctgtagtg atctatttgg agacacaaac ccttggtttg ccagtgaaga
     2641 agaaaaaaaa gaaaaacaac aatctgtcac atatgaacgc tatgaaatct tgcttcaaca
     2701 agcaactaac atgcaaaatc cgacaaaaaa agaaaccgcc ttaaactatc taaaaagtgt
     2761 caaggaatgg ttagatcagc aacaacaaaa ttgaatttga tttaaaatca tgttaaaatg
     2821 aatgtgcaaa tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg
     2881 gctttttttc aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc
     2941 aataactgaa taaagagaca caaactccta caagaagggg gcaaatgaac agttcaaaca
     3001 ttagatacac ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatatctta
     3061 aaaaattaaa tttgtgtggt gtttttaagt atcacttgtt ttagggttgt tagggcttgc
     3121 cctgaccgcc tgtaaggcgc ttctcgacta gattaaggga attaatcaag aattatctta
     3181 gaacggctta gacaagcttt cagagcctca cacctcctct ttgaacaacg gttttagcaa
     3241 aggagccgga ggcgactgcg cagtataata tgagagcata gcgatcacac ctttttagcg
     3301 accgtaggga gcaagttttc cagtttagaa aaattcgcga acctttttgg aggaaaaagt
     3361 aggagttttt taggcgggaa attcaatgtg cttttaagat atactgttag atatactgtt
     3421 agatatactg ttaataataa ctgttaggga ttagcaacct tatgggagag taagtattga
     3481 cataggtagt gggggataaa ttgtcacact atgggggata aattgtcaca ctatggggga
     3541 taaattgtca cactatgggg gataaattgt cacactatgg gggataaatt gtattttaca
     3601 tcctcttatg tctatgataa cataatttat tgaggtgatt tttattgagt aacgatatag
     3661 ttcgatataa taatggatta aattctgtcc ctttaagaaa atttacccct atagatatgg
     3721 atttattttg gggaatatgt tctaaaatga aaagaaaagg tacaaaagaa gaaattttta
     3781 catttaaaca aataaaagaa ttaattgatt acaatataaa tgcacgtggc gaccgttttg
     3841 ctaatgattt aataaaaatg tcaaaaaaaa tcattacttt gtcttttatt tatgaagacg
     3901 aaaaaacata tgaacaatta aatctgtttc aaaaatttaa aatcgataaa gtgaatgaaa
     3961 cattattaat aaaggtttct gaagaatttg aatatatact taattcaatc ggaactaatt
     4021 ttactcgttt cgaattagaa aatatgacaa acttaaagag tagctacgtt aaagagttat
     4081 atcgccaact tatggctcat aaagacagaa gatctaaaaa aggtcactgg tttgtaaaaa
     4141 tagaagattt tagagctcta ttagcaatac cagaaagtta tcgcatgaga gatattgata
     4201 aacaaatttt tgcccaagcg aaaaaagaat ttttaaacgt taattcaact caccctgcta
     4261 tttttaaaac ttttgaaatc aaaaaaatta aagcctacaa aggaaataag ataggatctc
     4321 tgcaaattta ttttgaagaa aatatacctt caattacctt agataattgg ttagagaaat
     4381 aatacctaac aatttaggag gcaattatca tgcaacccaa agaaaccttt acaataaagg
     4441 aattatctga attatttgaa ataagtaggc aagcaatctc taaacatgtt gataaattag
     4501 acccgtcctt attagctaaa aatgagcgtg gttataaggt tgtattacgt tctggagtat
     4561 tgcaacttgc tagcaactta ggtaaccaaa ggttactata taggttgcat gagtatgaac
     4621 aagaaaacga taaaaacgtt tatatatcaa gcaaaaacgt gattgtaaac gagttgctag
     4681 ataagttgcg tgaagaaaat gaatttttac gagaacaact ttccctcaaa gagcaaaata
     4741 attctaatat gcaaaaatta ctcgaccagc aacaacagct caccctacag gcaaatcaac
     4801 agatcgaaaa actgcaagaa cagttacaat tagcttatac ggaagaaact ccagaagatg
     4861 aatcaactac tattctttct gaagaaacag aatcgataga aaaacacaca aaaaagaaga
     4921 agcaaaaatg gtggcaactt tggaaataac tggagggaac attcatgaca agaaaaaatc
     4981 ttctattcgg ttctatttta ctaggaattc taggattcag tccgatgtta ttgtcagtaa
     5041 tcaataaagg gaataaacat ggtgaattga aattgaatcc aaattattac aacagtccta
     5101 acgaagaatt gtttgtaaaa gagacggata aataataaaa aagactgctc ttccgagcag
     5161 ccagttaaaa aaataatgag agtttattct tgaaaaagca acgcgcttat aaagcgctac
     5221 caaaatttta tcatatttta taaaaaataa caatccagta aaaaatgact ggtaaaaatt
     5281 cacaaagaaa cggaaagaag caactacaga aaatcaagcc ttaaaaagag ctttttacct
     5341 agaccaatta aaaatcacaa agtgagcgtc tctatccatt ggtagagggg caacttggtc
     5401 tagtgtacat tatgtcaact atgcaattaa ataactaact acttcatatt agcttaataa
     5461 aataatcaaa atcactaggg gaaaaaatat tgtataaaaa aatttttcat tttttaaata
     5521 agaatagtaa atataaaaat aatttaagat atctattact attaatccag atagagcaaa
     5581 ccaagtaaat attccgtgtg taaagctaaa aaaattcatt tgaatcaatt caaataaacc
     5641 taatatagta acaaatatat taaaaataat agctgtcaat accagtaaat tattttgttt
     5701 attttttaaa ttatcttcat ttaaaataga atttatgtta gtactataaa attcacaaat
     5761 tttttttagt gtctctatat ctggtgttgt tttgttattt tcccactttg aaatagtttg
     5821 acgtgtaaca ttaaattttc ttgccacata ttcttgagta tacccttttt tcactcttaa
     5881 ttctcttagc ttagatccta aatcctccat tttttctcct atgtaaacga ttatttacat
     5941 aaaattttat cataaaaata ctaacgcgcc aaaaaagttc tttattttat aaaacacact
     6001 aaaataaaat aattctagta aaaaagatag gagaaataag ttatgaaaaa atttttatgt
     6061 ctttctgctt taatgggtgt cttattaact agtggaggaa tagttagtgc tacagaagct
     6121 ttaaatctag atgtacccga agaccatacc gccatttacg gaggaggaat ggaagctaca
     6181 gaagaaggat tttcttctag taaattgaga tatgctgctg gcggcggaga tttcaactgt
     6241 ggggttaatg gatttaaagt ttatgcaaat tactaccatg ctagagctaa acatagtgca
     6301 acagctaaaa atggacgagg cggtcaagta cgatcagttc agaaagctgg agtaagagct
     6361 tatgcaacat gcaatgccac acttactgga aatactggtt ggtggaatgt ttattaaaaa
     6421 agagagttta aactctcttt tttatttaca tttgaaagga aattcaaaat atgttaaaaa
     6481 aaataaacgc tgttttatca ctttttgtct gcattacaat actattcttt tctactaatg
     6541 ttataaatgg tataaagact tctgaagcgc tatataaaaa taccgctgaa attcatatgc
     6601 aatataaaga tgatatggac tttaacagtt atataaacgc tttgtatgaa atatctacac
     6661 aagaaaatat taatatttct caatatagtt ttactgctgt taaccaacta actattcttg
     6721 ctacaaaccc taacgctaac aaacactgga aattttctaa aaaaaatata ttagcaagaa
     6781 acgattctaa agtccattat actaataaca aaaattctaa gtaccaatta aaacttccaa
     6841 ataatttttt aaatataaat atttatcctt tttctcatgt ggaaaatatt ggattaagtg
     6901 aaatattata tgtacaagga aactcaaaca aattaattcc tgtactgaaa aaatatggaa
     6961 cacctactat ttccaatatc aatcaatcag atacttttca aattaatact aatattgttc
     7021 ttgttatctg ctatcttagt atatttattg taattactac aattatattt gctttttcaa
     7081 aaatgaaaga aatcactcta aaaaaaatgt taggctataa ctcttttgat actataaaag
     7141 attctattaa agagttaaac ctcttatttt gttctttgat tataactttc attttatcta
     7201 tttttttgag catatattac agtaacgaaa attttttcct atcatttctt ctatgtagta
     7261 tttttcattt tataattagt tttttattta tcatagttac tctaagtatc tatcatttat
     7321 ctagtataaa aaattatcta aaaaattcac gaccacttaa gttaaatctt tgtatattaa
     7381 atatctgtct attcttaagc atgatccttc tgttaatagc ttctacaaaa gtaataaata
     7441 tccataatga agccgaaaat aattctctta aatattggga aagaactact aatttatata
     7501 aaacaaatat tactaatcaa ttaaatagaa acaacaccgt agaagaaaat aactacttga
     7561 aaaaagctag taaatttgtt tataaaatac agaagaatta taaaactttt attatcgcgc
     7621 catacaatta cgcaactatc caagaaaata ataaagaatt ttttattgga caaaaagttt
     7681 atccaaattt tgaagactat gttagtcaac ctgctggtgc aggaatatgt gttgatttaa
     7741 actatctaaa atattacaat cctatttctt ttgaagaatc tactgactta aatttaataa
     7801 actctgatcc actaactact tacattctag tacctaaaaa atataaagaa tatgcaaaaa
     7861 tgattgaaaa aaattattta gaagacatac agtttagact tgaagactct cctcctcaag
     7921 ctccttataa aactcctaat ttgaaaaatt taaaaataaa actaatattt gttaataata
     7981 accaaaagta ttttagtttt aataccttat atggaaaggc aaaagataac tatacaataa
     8041 ctgatcccat agttagagtt attaatccag aaataacaga atcattattt tggggaaata
     8101 tattgactag tgatggtgga ttattttttg atcagaaaca tacttcaaac caaaactttt
     8161 ttaatcaaat taatccgtat ataaaagaat ttaacctaga aagtatcata aatttttctt
     8221 attctgtttt tcaagaaact ggtgaattat tatctcaaaa aaaagtaaat aaattaaatg
     8281 catatctaca atatctttta atattaggat tatttataat aattaatttt caaatgattt
     8341 ttgttagttt tactataaat tcaaaaaaaa tagctttaaa aaaaatattt ggatattcac
     8401 caataagtca agttttagat ttatgtttac tcccttttac aatagagtta tttgctacta
     8461 tgtgttttca attgatattt tcaaaaaaca cttacttgta ttttataatt tttatgatct
     8521 ttttaataaa tctaattact tctattatta tttataaaaa aaatagtttt aaatatttta
     8581 aggagaatat tttatgatat tatcagttga gaatttcacc ctgtcaatta ataaaaaaaa
     8641 gatcaaacaa aattggaact tttcaatatc agaaaaagat tttattatag taacaggaaa
     8701 aagtggtagt ggaaaaacta ctctgttaaa taatttaagc ctaatagata aacaatatac
     8761 cggtaactta ttctattttg aaaagaaagt ttctaaacat gatatacaac gtctacgaag
     8821 aaatcatatt agctatttat ttcaaaacta tgctcttctt gaaaatcaaa cagtacaata
     8881 caatttcgaa ttagcaaaaa agtttaacag taatatttcc aatgatcata tttataattt
     8941 attaacagat ttttctttgc caaaagatat cttaaaagaa aaaatctttc tcttaagcgg
     9001 tggagaacaa caaagaatag cattaattag aaatatgtta aaaccatttg atatattatt
     9061 tgcagatgaa cctacaggaa atttagataa agaaaactca acttttataa tgaactttct
     9121 gaaaaattta acagaaaaag aaaaaaaagc cgttgtacta gtaacacatg acttaaatgt
     9181 tctaaactat ggaactaata ttatagaatt ataattttta cccgtgatgc tagctgattt
     9241 aaaactagca ccacgggtat ttattttata ttccatctct cctatcccaa aatttaaata
     9301 atcacttaat aaaaaggagg cacccaagtg attttattga aaaaagag
//
LOCUS       DAJSVF010000106         9050 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSVF010000106 DAJSVF010000000
VERSION     DAJSVF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.109"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..289
                     /locus_tag="ON550_002486"
     CDS             <1..289
                     /locus_tag="ON550_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955514.1"
                     /translation="VEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTR
                     SAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(386..820)
                     /locus_tag="ON550_002487"
     CDS             complement(386..820)
                     /locus_tag="ON550_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3955515.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1052..1762)
                     /locus_tag="ON550_002488"
     CDS             complement(1052..1762)
                     /locus_tag="ON550_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3955516.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1752..2612)
                     /locus_tag="ON550_002489"
     CDS             complement(1752..2612)
                     /locus_tag="ON550_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR3955517.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2617..3255)
                     /locus_tag="ON550_002490"
     CDS             complement(2617..3255)
                     /locus_tag="ON550_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR3955518.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3270..3569)
                     /locus_tag="ON550_002491"
     CDS             complement(3270..3569)
                     /locus_tag="ON550_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3955519.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3612..5096)
                     /locus_tag="ON550_002492"
     CDS             complement(3612..5096)
                     /locus_tag="ON550_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3955520.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5116..5577)
                     /locus_tag="ON550_002493"
     CDS             complement(5116..5577)
                     /locus_tag="ON550_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3955521.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="ON550_002494"
     CDS             complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="ON550_002494"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR3955522.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6880..7650
                     /locus_tag="ON550_002495"
     CDS             6880..7650
                     /locus_tag="ON550_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3955523.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7923..8750)
                     /locus_tag="ON550_002496"
     CDS             complement(7923..8750)
                     /locus_tag="ON550_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955524.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2760 a         1892 c         1409 g         2989 t
ORIGIN      
        1 tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa
       61 agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc
      121 agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa
      181 ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata ttagttcgaa
      241 tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg
      301 aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact aagctagaat
      361 tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc ttttttcttc
      421 tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat cctactgatt
      481 cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg actggtccat
      541 aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct tggcgctgat
      601 acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac
      661 gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca gattgttcaa
      721 atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata
      781 attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc ctttcttata
      841 caattctccc tctaataata cagttctttg actatttaat ctatcacata taactctcct
      901 gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat actttttgat
      961 aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat caagtgttat
     1021 cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca tactttccat
     1081 gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata ttggggttga
     1141 tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg ttttctactg
     1201 ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt actccaggga
     1261 cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc cctgtatgaa
     1321 cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga accttaccat
     1381 aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca
     1441 tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa gtttgataca
     1501 aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct attatatttc
     1561 ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg acaccgcttg
     1621 gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc caagtcaatt
     1681 tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc ttcattgctg
     1741 caattttttc ttcacaagac atcatagcca gcctccttca atttagaact taagtagtct
     1801 tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat ttcaatcaga
     1861 aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa atcaacacaa
     1921 ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac agggtaagta
     1981 tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg agccggatta
     2041 ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa aggtgaatga
     2101 atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc atccatgatt
     2161 tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt tattccttcg
     2221 ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta tccagctatt
     2281 tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat tagttgtttc
     2341 gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa
     2401 caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc agctatcttt
     2461 tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga catctcgata
     2521 aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat atctttgggt
     2581 aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac gaatgatctc
     2641 atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac cacgaccagt
     2701 gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc
     2761 agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata aatctttttc
     2821 accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta cctgccggat
     2881 accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga gttctacttg
     2941 caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac aacaaatgac
     3001 tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc cagcgtctgc
     3061 acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac agcgaatcgc
     3121 tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta ctacatcacc
     3181 aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg caatttgcaa
     3241 attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca ctttgtttca
     3301 gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat aactgtaact
     3361 ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa aactacatcg
     3421 tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt ggtaattggt
     3481 acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct tgttcctgat
     3541 ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt gttgtctaat
     3601 cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc tacaatcaaa
     3661 aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa accaatgaca
     3721 actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa tggccaagct
     3781 gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat ccatgtagcg
     3841 aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag gcaagcggct
     3901 ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa gaacaacgga
     3961 atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa
     4021 aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga accaaagcca
     4081 aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt tgagattcct
     4141 tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa gatcgccaga
     4201 tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg cccttcttgc
     4261 atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat cccaaagaaa
     4321 aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa gaatcctggt
     4381 aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt ttcatcccgc
     4441 tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc
     4501 actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa gttagaagct
     4561 actgcccaat aacaacctaa aatcagtccc ataaaaatca atacttgtat cctacctaga
     4621 tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt
     4681 ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa taagatattt
     4741 aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc aaatgtctcc
     4801 attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat ggcatccaaa
     4861 ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa accaccagag
     4921 ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat gcattcatga
     4981 agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag gtatgctggc
     5041 tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc ccccatcttt
     5101 tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat cgatggagtt
     5161 cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata atttttggat
     5221 attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt
     5281 ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg taaatgaaat
     5341 agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta cttgaggtgc
     5401 gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca catacgtttc
     5461 atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt cacgccaatc
     5521 cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa
     5581 actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat cgagaagaca
     5641 tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg atagatgaat
     5701 ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc ctttttgaat
     5761 ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat atcgtagtgg
     5821 acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc aacagaagta
     5881 actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac atcgattttg
     5941 tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc atgataaaga
     6001 ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc ctgaggcatt
     6061 tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg atcaaacgct
     6121 tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt tactgtaatc
     6181 gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg
     6241 aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc tagatgatcg
     6301 gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg atcgattacg
     6361 aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg catcatctga
     6421 tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca ccatagatca
     6481 cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc cagccaccac
     6541 atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa gtatgtgccc
     6601 cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac agttttactc
     6661 atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa tgtgtatcag
     6721 catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct ttcagtaatc
     6781 aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa ctagtcaaat
     6841 aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat
     6901 attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca actatcaact
     6961 ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga tttaaattta
     7021 ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa ttacaaaaac
     7081 cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc cagagaagct
     7141 agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa
     7201 acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt gaaaatcaat
     7261 tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat gcgctttcca
     7321 aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa tgcagacatt
     7381 tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa gatattaaat
     7441 gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat tttagtcgct
     7501 gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc agtctttgat
     7561 tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag aaatacgggg
     7621 ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt aaataggatt
     7681 atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc tcaaattgat
     7741 aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg cttcttttat
     7801 aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag gtatgacaaa
     7861 agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat ttcacaacca
     7921 ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt gaaacgcgct
     7981 attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg cttatggcaa
     8041 gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt cttattatac
     8101 tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt acggtaatag
     8161 gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt ctcactcaat
     8221 attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt tgacaagtag
     8281 atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc
     8341 aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag atgatcaagc
     8401 agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt ttcctgatcg
     8461 atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa tgattgaatc
     8521 tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg
     8581 acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt
     8641 tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac
     8701 ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc
     8761 ttccctttaa aaaattattt ctcctttata tcatacacaa aaaaaagttc taaacaacca
     8821 aaaatgttta gaacagaata aaaattttaa ttttaaaata atattcaaat taaacaactg
     8881 gcgtatctgc tccttgattt ctttttgtgt tatctgaata attatcgttt acagttataa
     8941 cacatagtga aaattgtcat ggcgagaaag tctaagaata tttttaaaca taactatttc
     9001 tttcttacgt gtgtataatg acgacagcac acctatcaat taatataatt
//
LOCUS       DAJSVF010000107         8777 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSVF010000107 DAJSVF010000000
VERSION     DAJSVF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.110"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..341
                     /locus_tag="ON550_002497"
     CDS             <1..341
                     /locus_tag="ON550_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955525.1"
                     /translation="FSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPK
                     SISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKN
                     NNFYFYRNKL"
     gene            complement(467..1144)
                     /gene="nth"
                     /locus_tag="ON550_002498"
     CDS             complement(467..1144)
                     /gene="nth"
                     /locus_tag="ON550_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR3955526.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1151..1849)
                     /locus_tag="ON550_002499"
     CDS             complement(1151..1849)
                     /locus_tag="ON550_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3955527.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2117..3301
                     /locus_tag="ON550_002500"
     CDS             2117..3301
                     /locus_tag="ON550_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR3955528.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3307..3750)
                     /locus_tag="ON550_002501"
     CDS             complement(3307..3750)
                     /locus_tag="ON550_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3955529.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3976..4263)
                     /locus_tag="ON550_002502"
     CDS             complement(3976..4263)
                     /locus_tag="ON550_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955530.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4434..5786)
                     /locus_tag="ON550_002503"
     CDS             complement(4434..5786)
                     /locus_tag="ON550_002503"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR3955531.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6059..6505)
                     /locus_tag="ON550_002504"
     CDS             complement(6059..6505)
                     /locus_tag="ON550_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3955532.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6741..7667)
                     /gene="cysK"
                     /locus_tag="ON550_002505"
     CDS             complement(6741..7667)
                     /gene="cysK"
                     /locus_tag="ON550_002505"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR3955533.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDTVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7807..8694)
                     /locus_tag="ON550_002506"
     CDS             complement(7807..8694)
                     /locus_tag="ON550_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3955534.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2391 a         1790 c         1450 g         3146 t
ORIGIN      
        1 ttttttctgt acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg
       61 caatgattct cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc
      121 tgaaaccgcc caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac
      181 ttatcatttt tattacacct ttagatagat acgtcactta tttatataat cctttggaat
      241 actggactta ttttataggt atattggaag gcattatttt ctacatcggg tattttccca
      301 ataaaaataa caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta
      361 ctgagtatgt acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata
      421 ataacaagat aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt
      481 tcttttttag cgccttttct tttagacgca tccgattttt cccatcttga catattgata
      541 gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa
      601 tcagtgtatg atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt
      661 ctacttccat gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca
      721 cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg
      781 tctttctgcc tacaccagga agagacatca attcttctct tgacgttggc acttgcccat
      841 cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca
      901 aaccaatcgt ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg
      961 gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct
     1021 gggcacttaa gatcactgca atcaataact caaatggatt attgtgcttg agttctccat
     1081 gtgcttcggg aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta
     1141 acatattccg ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt
     1201 gagctgactg ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt
     1261 ccacttgttg ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt
     1321 aattaaaact ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg
     1381 gctgataatg gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg
     1441 ggcgtccaaa ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt
     1501 tttcttcgta ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt
     1561 aaagattata tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc
     1621 catttgttac gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca
     1681 tagcaatcat ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta
     1741 gaacaaataa aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat
     1801 tagatatgac tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc
     1861 cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt
     1921 tacttcactg atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt
     1981 ttgatgcgta tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga
     2041 atgttgacaa ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc
     2101 ataaaggagt ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt
     2161 ctctgtctta ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac
     2221 gctgccttat tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt
     2281 ttatgctcta gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg
     2341 gcgtcatccg atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg
     2401 tttaggaaat tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg
     2461 agaaatctcc accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa
     2521 agtttttgga tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg
     2581 gggtctatta gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt
     2641 cttaaatgcc gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc
     2701 agtagattta agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat
     2761 ttcatccgtt attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct
     2821 tcaatctatc ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg
     2881 tttaagcttc tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg
     2941 attgctcaaa aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct
     3001 cgcctatcta ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg
     3061 gattatctgt tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc
     3121 caattctgtc tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc
     3181 tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct
     3241 tccgacggta ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata
     3301 gtagcttcat ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt
     3361 gtcaaattct tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg
     3421 atggagttgc tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat
     3481 ttctttttta ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt
     3541 tgttagctta ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt
     3601 aggatcaggg attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc
     3661 aaattctttt tccatttctg gtttattacg atgaataatc tcattagcta aatccagata
     3721 ttcttctatt agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt
     3781 gtttttttgt ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa
     3841 ccaagaaaaa aaggggttga caaagcttgt cataacccct ttttctgatt aaaagatagt
     3901 aaagagacag tcatcggtaa ttgtgatgat tttttgtcat cacttaagga tgagcggcct
     3961 tttcaaaaac tgtctttaaa gatcatcatc agtttctagc acatatggct gaccttcata
     4021 tgtgtactct gtattcaatg tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg
     4081 aaaacgatga gaaacattat ctttggcgtc atttgctggc atcgtgtaat attcctcttg
     4141 cgtttcattc aactgagaga gtacttgatc tgccaactca tcaatattga tcgttaattt
     4201 cttcgaagca cttttataat cttctaaaaa tgcatcttgt tggacaaaat attctttatt
     4261 catcttcttt ctcacctttc ttttcaaaat caaataaagc tgcttgttct tttatgtaac
     4321 agctttatct ttgctgctta ttgtgacaaa aaattcgaag aaagacaact aattgtactt
     4381 ttcacaaaaa aagacagagg gtcgtgacaa aattgtgtca caaccctttt tctttattgt
     4441 gcagtttcga tttcgcctag cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg
     4501 tcaaaatgtg gttggaagaa gaaatctgaa agtgcgagat cttctacagt cattttattt
     4561 tgaacagcta aagacatggt atttgctgat tgtgtaatgt cgtatttgga catgaactga
     4621 gcacctacga tacgattcgt tcctttttca tagactagct ccatcattac tttttcagta
     4681 gttggcatga actctggacg ataattatct tcaaatacag tcgcttggac ttctagatcg
     4741 ttcaaagttg cgctttcttt ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa
     4801 agtcctgaag ttccttgagt accacgatac gccattttat gatctgtcaa gttatttccc
     4861 acaagcaaac cttgacgaac ggcatttgtt gccaaaggaa tatagttttg cgtgccactt
     4921 ggattgtagt tgactactgc agagtctcca gctgcaaaaa tgtctggatt acttgtctgc
     4981 atgtattcat tcactttgat tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt
     5041 tttgtatttg gacggaagcc cacacataaa atgaccatat ctgtatcaaa tgtatctgtt
     5101 ggtgtagcta cttgtttcac ttttccttct tcatctgtga taaattcagt aacattttcg
     5161 cctagcgcaa gagtaacacc gcggtctgtc aattcttttt ctaagatatc tgtgaatggt
     5221 ttatctagat atttattcaa gatgcgatct aatccatcaa tcaatgtcac ttgttttcca
     5281 gattcgacaa aagcttctac taattcaata ccgatatatc caccaccaac aattactaca
     5341 cgttttgctt ctttcgcttg tgcaataatt tcatttgctt gattgtagtt tttgcatagg
     5401 agtacatttt tcgcatcgat tccactaatc ggtgggataa taggccatga accagttgtc
     5461 atcactaatt tatcataaga aaccgtttct gtttctcctg ttttcagatc ttttgccgtt
     5521 actgttttat tttctgtatc gacgtctgtt acatcgtgtt ccattttaac tgttgctcct
     5581 aatgaagata attcttcagg atttgagtag aacaagcctg ctgggtcctt tactactccg
     5641 ccgacataaa gtgcgatacc gcaagataag aaagaaatat tgtcatttct ttcatatact
     5701 gtcacttctg cttctggatg attttttaaa atacttttta cagctgcagt acctgcatga
     5761 gtacagccaa ttactactac tttcatttat ggtccctccg attaacattt tgtccttcga
     5821 caaaactact ataacagaaa taaaacggat acaacagttt aatgcttgtg aattcacaaa
     5881 ggtttgtttg tagtttcatt ccttttcaag cataagaacc caatatctac aagattctta
     5941 ttttaataat attcactttt atatctaaga ttgtgaataa aagacaaaaa aataaaaaag
     6001 ctagcgtttt ttttacacag ctagcttttt ttaggtataa cacttgtaga aactgttttt
     6061 acatttgcgc cttttttgct gactctttac aatccggaca gagtccatac aattccaacc
     6121 gatgttcatt aatctcaaaa tcagtcagtc tgcttgcagc catttctata tcttcaagcc
     6181 ctgggtaatg aaaatcaaca atcttaccgc agttttcaca aattacatga taatgacgct
     6241 ttgaactgaa atcaaatcgg ctagaagcat cgccgtactt catttcttga acaaatccaa
     6301 tcgaagtaaa taagcgaagg ttattgtaaa cagtcgcaac actcatattt ggaaaccgat
     6361 gttcaagtgc tttataaatc tcatcagccg ttggatggga atgatgttca atcaaatatt
     6421 ctaaaaccgc gtaccgttga ggtgtgattc gaatgttctc tttttttaac ttttcgataa
     6481 cctcttcgac taacttattt tccatgtaaa caccccacgt tgttattctc gtcttatcat
     6541 actattttta cattttaaaa tccacttaac atgaaaagaa aattcaattt tattgtattt
     6601 taatgaaaaa agacgtaaaa cagtcgcgtt cactattcta cgtctctatt caaataattt
     6661 ttctcatcat aaatttttgt gtgttctttt catctttaaa agttttttac ataaacctat
     6721 ctggagaaaa accaagtgcc ttatatctct tggtaaaggg ctgtagataa ataacgttca
     6781 ccattgtcgg gaaccacagc taataccttc ttgccttttc ctaattcttt tgcgactttt
     6841 agcgcggcag caattgcagc gccagatgag atgcctacta aaatcccttc ttttcgccca
     6901 acttctctag ctgtttccat cgcttcgtct ccactaatag aaaggactgt atcatacaca
     6961 gaagtatcca gtgttttagg aacaaaacct gtaccgattc cttggatttt gtgaggtccc
     7021 gcttctttcc cttctaaaat agcagattct gctggttcta ccccgattaa ttcgattttc
     7081 ggatacacac gttttaattc tcggccagcg ccagtgatcg ttcctcctgt accgatacct
     7141 gcaacaaacg cgtccaagcc atttactcca aatgcttgat gtatctcagg accagtcgtc
     7201 ttctcatgca caagaggatt tgcttcattt tcaaattgta atggtaaaaa gtagccgttt
     7261 tcttttgcca aacgttctgc ttctctgatt gatccagaaa taccatccgc accaggagtc
     7321 aaaatcaact ctgcgccgta ccctttcatc aataaacgtc tttcgatact catcgtctca
     7381 ggcatcacga tgattacttt atatcctttt gcaactccta ccatcgacaa tccgattcct
     7441 gtatttccag aagtaggttc aatgatagta tcacctggtt tcagtagacc atcgtgttct
     7501 gctttttcta tcatacttaa agcaatacga tcttttacac ttcctcctgg attaaaaaac
     7561 tcaagcttta caaaaacatc tgctgaatct tctggtacga ttttatttaa tttgacgatt
     7621 ggcgttttgc caatcaattc ggtgactgag ttatagatag ctgtcatctt caattcctcc
     7681 ttgtctttgc tcttcttact tatcataata caatctgttc atttatggcg agtgattcgt
     7741 ctttgactag caaaaaaaag acaaagatat accatatatg gtaatttctt tgccttttta
     7801 tctaaatcaa acgtttagtt cttgaccaac taataaagaa taactggaca tagacatgcc
     7861 atttttcgcc attagatcat caacagacat gccaaatttt tgtgcaatat cccatagcgt
     7921 atcacctgac acgactgtat atgtctgacc attatttgta tttacatctg ttgttgaaga
     7981 tgatgacgtt gaagtcgtcg tagtagtcgt tgttgttgta cttgtggttg ttgtagccga
     8041 acctgttgat ggcgtatcaa aacgggttaa attataagta ttaatcaatg agttcaattt
     8101 ggccccataa tttggatcgg tagcataacg acctgtcaag tacgctgttg catcataaaa
     8161 acttgttgta ttgcttttcc acacgccagc ataatgataa tcgcccgtgg aaaaactggt
     8221 tgaacgaaga acattcgcat ggtcttggaa ggattcccaa taagaagaat attttcggaa
     8281 tggctcattc atcgttaccc attgtccgtt tacatattct ttggttgcca tataaacgac
     8341 ctgtccgtta tagcttcctt tcacaccgaa tagattataa tttggcgcac tagctaatcc
     8401 ggaagtgcca tatgcacttt ccaacaacgc ttgagcgatc atgactgaag catacaagtc
     8461 attacttgca gcaacttctg acgcagacca gccgatttga tcgataaacg cttgaggatc
     8521 agcgactgtt tgagatcctt gttcatctgc atgaacatgt tgcggtaccg ctgtaaccat
     8581 catcggccca actaaaagac tcgttccaat taaaggagca atcgcttttt tattgatcac
     8641 catttttctt ttaggtctat ggtgccgggc actgcgggtc ttcagctctt gcattcaaat
     8701 tcctcctcaa aataaaaaaa cttaataaaa agttctcttc ttaaaacaaa cttaattaat
     8761 taaattatac ttttaat
//
LOCUS       DAJSVF010000108         8585 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSVF010000108 DAJSVF010000000
VERSION     DAJSVF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.111"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(321..851)
                     /locus_tag="ON550_002507"
     CDS             complement(321..851)
                     /locus_tag="ON550_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3955535.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1033..1689)
                     /locus_tag="ON550_002508"
     CDS             complement(1033..1689)
                     /locus_tag="ON550_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3955536.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1816..2289)
                     /gene="dfrF"
                     /locus_tag="ON550_002509"
     CDS             complement(1816..2289)
                     /gene="dfrF"
                     /locus_tag="ON550_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCR3955537.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2708..2926)
                     /locus_tag="ON550_002510"
                     /pseudo
     CDS             complement(2708..2926)
                     /locus_tag="ON550_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2957..4171)
                     /locus_tag="ON550_002511"
     CDS             complement(2957..4171)
                     /locus_tag="ON550_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3955538.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4512..4754)
                     /locus_tag="ON550_002512"
     CDS             complement(4512..4754)
                     /locus_tag="ON550_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3955539.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4786..5343)
                     /locus_tag="ON550_002513"
     CDS             complement(4786..5343)
                     /locus_tag="ON550_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3955540.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5356..5544)
                     /locus_tag="ON550_002514"
     CDS             complement(5356..5544)
                     /locus_tag="ON550_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3955541.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5557..6123)
                     /gene="amaP"
                     /locus_tag="ON550_002515"
     CDS             complement(5557..6123)
                     /gene="amaP"
                     /locus_tag="ON550_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3955542.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6540..6713
                     /locus_tag="ON550_002516"
     CDS             6540..6713
                     /locus_tag="ON550_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955543.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            7353..7826
                     /locus_tag="ON550_002517"
     CDS             7353..7826
                     /locus_tag="ON550_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR3955544.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7835..8062
                     /locus_tag="ON550_002518"
     CDS             7835..8062
                     /locus_tag="ON550_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955545.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2611 a         1685 c         1273 g         3016 t
ORIGIN      
        1 aatttcttat tttttgaaat caactgtctt tctcatattt actattcaat tttcatagtg
       61 atataggtac aatttatatc actactgtaa aaatggggtt ttgccttata aatacagcgt
      121 tttccgcttg ttagtgtata gtgtttcttg ttttttgtgc cccccttgtt agataacggg
      181 ggctttgaac ggctcgcaag ctcgccgaac cgccgaagcg aaccgccccc ttgcaagcat
      241 ggcggaaaaa tcttaaggat tttcccgcca acgtggtcgg atcgctccgt cggtttaaca
      301 gcttttctgt ttctctctag ttatctacgc atataaatca aatcggttat tccgttataa
      361 gtctgtgttt tacaaagcct taactttatt tccctgtcta tgttgcccaa aagacgaacc
      421 ccatttccta acagagtagg aataacagaa atgtaatatt tatcaatcat atttttgctt
      481 accaactgct gaacaagatt tgctccgcca caaatccaaa tatcctttcc tgcttcttgt
      541 tttagccgtt ccaataataa aacaggattt tcacttgtaa aatgaatttg ttgggaagaa
      601 ttgcactctt tatgtgttaa tacataagtt gtaaatttat tatataccca ttcttgcgga
      661 gaaagttcag taacaacctg atgagaagta ttccacccca ttaagacagt atctatatct
      721 ttggtaaatt ctgtataagt gtcgatgttt tcattgtcat ttccatgacc atttagccaa
      781 gctactccac cattaccgtc cgcaatatat ccgtcaaggc tcatggcaat aaataaaact
      841 acttttctca ttttttctct tttataaata caaacaggaa tttatttctc ttttttactc
      901 ataagggaac aactgattgc tacaaataaa cccattaaaa tccatggaaa tgctgctctt
      961 ataaaatcac taaccatact ttattcctcc ttaaattcca atttccttag ttctacaaac
     1021 tgaaattttt gcctatgaat gtatcttgtt ctttatacgc aaacaggctt ctgtgtgcat
     1081 aatccaatgt cttgaaaacg gcatttgaat tagtccacaa atatctttat tacaccaata
     1141 atcaatcagc caaattgctt tttcattgtc gtttactaca ttcaacgatt ctaagtatcc
     1201 ttttctttct ttcggtattt ttcttttcaa ttcatcataa gataaccgtt caagcatttt
     1261 ctcagtgctt tcccatactt cgaaaatata taaatataat tcttccagat taagctgttt
     1321 tgaaaagtct gcaatctgct gtttcatgag ttcatttcct gttgtaatga taggtgaatt
     1381 gatacgctct tgataattgc ctgcaaaaaa tacttgttca tcttcattta tcactgtatg
     1441 cacaactata tcttcaatgc ggaaaatatg ccaaatagaa taagcaatcg tcttactatg
     1501 ataaccatca gcgtttatga aggggattga gttaaaatct tctctgcata attcttcttt
     1561 gaaagatact aaggtttgta ttaactgact tcgtaaagta agtaaagtat caatacccct
     1621 cttataagta tctttctttt ttatttgtgc ctgcattgtt ttattaagtt cagaccattc
     1681 cttattcata ttcaatcctc tctcaaattc tgattttatc gccccataca aactggaatt
     1741 tgtcttacaa caatttatat atgcataaaa agcaatatat ttatgaaaac accctaaatc
     1801 caaaatttac tcaattcatt ttttccttac ataaaatgtt ctcgtatatt tcacttcctc
     1861 accaagtgtt tcccctatca aaacttcaaa atcattgata tcaaattctg gaaagaatgt
     1921 atctccatct tcaatattta aatctacttc tgtgatatac attttatcta ctatttgcaa
     1981 agcttcctta aatagtccat atccaccaga tatgtataca tctcgtcctt tagccaataa
     2041 taatgcatct tctaatgatt taactgaaac taaattatct ccttgatact ctgttgtggt
     2101 ggaaacaaca atattcattc tattaggcaa cggatgaccg atttcttcat aagactttcg
     2161 ccccataata accacattac ccgttgttaa ctctctaaat tgcttttgtt ctccctttat
     2221 tttccatggt atattaccat tcttgcctat aacattattc tttgacctcg caacaatcaa
     2281 acctatcatc tatttcatcc tccataatat gatgtcgtac ctgtgattcc atcaagtgca
     2341 ccacatctta acaaactcaa aattatctcg aactaaccag ctcgacaaat tccttttttg
     2401 tcttcatctg cattatatca tattgttata agcattgcca tttcttttct gcttttgaaa
     2461 agttacttat cagcctatgt tttgtaaagg gtgatattca gcatttctca cgcaacaagc
     2521 ccttgacaaa ccatagacta ataagtttta aggtaatcaa gcagaaatga aatagtctgc
     2581 tgtttcacgg caacaaaaaa gagataccaa cacacttatg tagtatctct tagtctagta
     2641 gcaatattct tttgtttgca agcaggtaaa catgggctgt ttcttaatat ctttttgttt
     2701 gggaaacacc tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag
     2761 ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg
     2821 cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa
     2881 agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt
     2941 tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt
     3001 cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg
     3061 ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga
     3121 agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa
     3181 tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt
     3241 gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca
     3301 gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa
     3361 cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca
     3421 gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt
     3481 gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc
     3541 ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct
     3601 ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga
     3661 ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc
     3721 agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt
     3781 ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca
     3841 tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga
     3901 agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat
     3961 cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg
     4021 agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa
     4081 aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg
     4141 tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag
     4201 tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat
     4261 aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa
     4321 taaagacaaa aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt
     4381 atttttaaag atattttttc ataaggaata aagaatactt catataaaat atataaaaaa
     4441 gaaccgcagc gagtgtcgct acggttctca aatttgttct tcatttcaac ataagttttt
     4501 cttcaatttg cttataattt acgtgcaaag aatgaaacga ctaatactaa aatgattgca
     4561 ccgatcaatg atggaatgat agccattcct gcaattgttg gtccccaagt tcctagtaaa
     4621 gcttgtccaa ttgctgaacc tactaaacca gcaatgatgt ttgcgatgat ccccattgat
     4681 gatcctttat tcgtgattgc tcctgcaatt gctccaatga taccgccgac gattaatgcc
     4741 caaataaatc ccattttaaa ttcctcctaa ctgacttctt acttcttatt gtacacgtgg
     4801 ttcgttattt tctttgactt tttgtgcacc tttgcctaca gcagattttg ctttatctgt
     4861 ttggcgtgaa gcaaattgtc ctgtggattt tgctgcttct gttactttgt cttgtactgt
     4921 ttcttgatct ttttggtatt cttcttgtgt tttgatgtct gcaacattca cattcacttc
     4981 gataacatct aaatgtgtca tattgtttac ttcagtactg atcaattctt tgatttggtt
     5041 gtaaatatct tcgatgtctt taccgtattc tacgatcaca tctagatcca cagctacttg
     5101 tttcttacct acttctgtat tgatacctgc tgttacgtta tctgtgttga ctaatttttc
     5161 agccatattt gagaaaaatc cgccatctaa tgtcaataga ccatctacat ctttcaatgc
     5221 ataaccgatg attttttgga tgactttgtc ttcataagtc aattcaccat tcatcatgcg
     5281 gttttctcct ttttcatttg ccaaaactga tccatttgtt gtattcatgt ttttgttttc
     5341 catctttgtt tcctcctatg atttaaaaaa ttgatcaact attcctgact gtttcaaata
     5401 aaaaccgatt cctgcgccaa ttgctgtcat gattatgatc aacaatgtct tgaaaaatcc
     5461 aatcgttaac cataaaacag ctagcagtag tccaagaccc ccaaagataa tagggagctt
     5521 gtatgctgaa aatagttctt tcattatttg gccctcctat tccactcgag agtgcttgta
     5581 gtctcttgta tcttcttgtt cttcataacc gctgtatgtg actgccactt tgaccgtttc
     5641 ttttgttccg agtactttag ttacttcttc ttcgatatct tgcatcaata cacctgtttt
     5701 ccctactaat gaagaagttc gtttcaagtc tccgttgaca tggatatgga ttcgattttt
     5761 cgttgaacgt atccgtactt taggtgaatg aatgaactct tcttcgtgta aatgggaacg
     5821 taccaaccct tcgatcgctt ttttgtcgat tgttagtttg ccatcttccc gtttcataac
     5881 gaaagttcct cttgactttg gatagaaaag aacgactagc aagatgataa tagcaagaac
     5941 ggcaaatact acagctgccc agaaaatata ttgttgcatg tagtaattcg ttaaagtaaa
     6001 gaagcgaaat cgttccaatt gttcaggcat aatatacggc gcattgatca acgcaatcgc
     6061 tagaaatact gaaagtagca gtaaactcat gataacaccg attattttta accccttatt
     6121 cattttccac ctcctataaa cttattttta ccctgtaaat aactctaccg acaatcacag
     6181 tatagatagc gaacagttag catagcatca gtaaataaag cgctaccaaa cattgctgga
     6241 tcataacggc gttttattgc cagtctgata ctgaaataac aattgcttac ttattctcta
     6301 tttacttttg attgctggct atacttaaag gatattattc aagattaaaa attgcaaaga
     6361 ttatgctcag ttattattac aagattctat ttatttatta aattttttcg gagaattaaa
     6421 aaatacaata aataacttta gttactttaa tgcgttctta ctttattttt atattatcat
     6481 ttttcttatt cacttctttg gtttatacta ttcttataaa taaaaacgag gtatgtccta
     6541 tgatgaaaag attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc
     6601 taggaaatat ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat
     6661 ctctattctt tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg
     6721 ccatctatct gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt
     6781 tatttttcta cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca
     6841 tctacttgcg cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg
     6901 attttccttt tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca
     6961 aaccattgaa taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga
     7021 atcggttagc tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct
     7081 cttcgctaga taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa
     7141 actagcatta tagaagagac acacatagaa tctaaaataa ctggttttaa atgtttctta
     7201 caatcaaaaa aagctgttaa atgcactcca caccttcata ctctatcatt ttttataaac
     7261 gatatatttt tattgttttt cgataaaaaa tagttgtttt acaataatta ttctgctata
     7321 ttttagctat caaagataaa agagggataa aaatgaaatt taaaacattg ctattaaaat
     7381 ttgtcgtttt aggtataagt gccgtattta ttttgttcgg cgtacttttt tatactcaga
     7441 tcgctacaag cgaaaaagga ttcaattttg attggcagac cacattattt gtttctgtca
     7501 tttactttgc ccttctccta gggatcgtta ttgcttattt cttattgaaa ctattgaaaa
     7561 aaatcgaaga taaccaagct ttttccactc aaagcttaaa catactaaaa aaaatcgaac
     7621 gaaccatttt gtttattggt ttcgcctttt ttggcttact tcctaaattt atgaaagtag
     7681 cggatttaga cgatgctcca ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt
     7741 ttgcagtata cacattaaca agcgtgctgg aaaaactgct tgaacatgcg attttactta
     7801 agacagatca tgatttgaca gtgtgaggtg cgacatggca attatcgtta atttagatgt
     7861 agtattagct caaaaaaaaa tgagtgtgac tgaattgagt gagaaagtag gcattacgat
     7921 ggcaaacatc tctattctaa aaaatggaaa agcaaaagcc atccgttttt ccactctcga
     7981 aaaaatctgc caagttttgg agtgtcaacc aggtgatttg ttgacctata aaaatgactc
     8041 gaatactata ccagaagaat aaaataaagt agaacaaaca gtaaataaaa aataatctga
     8101 atgatagatg tgtgagactt ttagaagtta tttacgtcta ttattcagat tttttatttc
     8161 gattgaatac cgttttatct ataaataaga tatgtactga ctcatacaaa gggggccttt
     8221 tcttctccat gtactatcac ttttcctgat acaactggtt tcctgttacg ttgtagttgg
     8281 atctgctttt agtataatac tttattcatt ccaccagact gatagcagtt tgagcagtct
     8341 aaataatcat gtagtattac aggccattct gtttcatacc ttgaagtgct taaaacttca
     8401 gctaatggcc cattgtgtta actcttactc cattcttttt tagtaagctc ccatatatac
     8461 tgtaaagcct tctatatatt atgtcatgtt gctttttaac atttactatg tagttttgat
     8521 aaaatgattt gaataacagt gatggacaat tcttttttat ttttaacatt attttgataa
     8581 taatg
//
LOCUS       DAJSVF010000109         8493 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSVF010000109 DAJSVF010000000
VERSION     DAJSVF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.112"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..172)
                     /locus_tag="ON550_002519"
     CDS             complement(<1..172)
                     /locus_tag="ON550_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3955546.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(592..1713)
                     /locus_tag="ON550_002520"
     CDS             complement(592..1713)
                     /locus_tag="ON550_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR3955547.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2041..3450
                     /gene="istA"
                     /locus_tag="ON550_002521"
     CDS             2041..3450
                     /gene="istA"
                     /locus_tag="ON550_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR3955548.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3447..4175
                     /gene="istB"
                     /locus_tag="ON550_002522"
     CDS             3447..4175
                     /gene="istB"
                     /locus_tag="ON550_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR3955549.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4303..5214)
                     /locus_tag="ON550_002523"
     CDS             complement(4303..5214)
                     /locus_tag="ON550_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3955550.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5231..6238)
                     /locus_tag="ON550_002524"
     CDS             complement(5231..6238)
                     /locus_tag="ON550_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3955551.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6235..8364)
                     /locus_tag="ON550_002525"
     CDS             complement(6235..8364)
                     /locus_tag="ON550_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3955552.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8369..>8493)
                     /locus_tag="ON550_002526"
     CDS             complement(8369..>8493)
                     /locus_tag="ON550_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3955553.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1726 c         1485 g         2683 t
ORIGIN      
        1 ttaaaaagtt agtaaataat tgccctctaa gttccacggt ctgttcatta ataactttat
       61 atcctcgttt ttcaaaaaat ggctttgcgg taatggaagc gtgggtagtg attttttgca
      121 taccagtttc taaaacatgg ttttccaatt catctgcaat tttagtcgca ataccttttc
      181 cttgatggct atgtttttgt cacaacttca gtattcctgt aacaatacgg atttcagtat
      241 acaacaacag cttttgaaaa gatacttgca aaaggattaa gatatctatg acctgctatg
      301 ctcactgatg ttgaaaagaa tgttttatta ggattgaggt aagcaagata gtgaatatgt
      361 ccggaaagcc tgtaaaataa ggcttttcgg acatatgtga agttataaaa agatagcgaa
      421 aaatctattt agtatcacta atgttgcgaa catatcgtca gaatagtctg acatttattt
      481 cttgctaaaa atttcaaaaa agtcaagtgc tgacgcagcg gatgactatt ttccagaaaa
      541 ttttagtatg tattcaaaaa tcaaacgtga caacagctat taactacatc ctcatccagg
      601 tttgtaacgt ttgaaccact cttctgcggg ctcaaacatt aagtaatgga aggtacgttt
      661 caattgaaac agcgtcgctg tcgtattcaa ctcaattttt atcacataag tgagaagcgt
      721 tgcgattaag gtgaggatga cttgatttat cgccccttgt tcactatgtg aatatagtcg
      781 tttaatggtc agattttgtt ttaagtgttt gaacaacaat tcaatctgcc aacgggcttg
      841 atacatgtcg gccacttcgt ttggcgatac atcaaagaga ttggtcacca ttcgcaggat
      901 tttttgtccc ttggtaagaa ccgttaccaa cctaaaacgc tctgtcacat ggttgacacc
      961 gccgacaaga atcacttgtt ggtcactgat tatcgtccca tcacgggttg ttgtatctgc
     1021 tacttcaatt tgatccaaga tggtaatttt tgtattagat tttgtccgtg taacgaagaa
     1081 atagccgtca cgatttagtt tgtctaacaa cttgtaatca aagtagccgc gatccatcac
     1141 atacgttgct tgcgttttat caacaagaac ttcaaggtga ttacggtcat gctcactcgc
     1201 atttgtcatc gtaaaacttt caggatactg attggtttta tcaagataac agagattcaa
     1261 atgtagcttg atgccagact ttgttttccg aaacttggcc catggaaacc atgctttgtt
     1321 caacggaata gtcgttgaat caatcaattg tagactggtt gtttttgagc ttggctgctg
     1381 agcagaaatt ctggcgacta attgcgtaaa aatttccatc aatacttctt gcttgatttc
     1441 tggcgctcta cgagataacg agctgtaaca cagggaatca atgccaagtt ccttgcagag
     1501 gcgctgatct aaaaaggcgc gatcaatctc gcgatagctt ggcaattctt cataaaccgc
     1561 atgaagtaac actttaagcg tcgtttggaa gtctagtttt ttactgtaga aatcaaagtc
     1621 gctaattaag ctttgagagt tttctgataa accagaaaaa tttattgcag aaaaccattt
     1681 ttgaaatgag gtttttacgt tatacttatc catgcgtgag tcctttttat tggtcttgga
     1741 taagtcatcc gagaatagta taaaggactt ttttgcgttt ggatagcttt gtattaggct
     1801 tttgatgggt taaaaagtct ttgcaacact agtaatttag tatttatgta taaattgaaa
     1861 gatattttac caccacttag tcttcttaaa agtgatccaa cattttgttt gtggcagaat
     1921 ttaactacac actaatgcag ccgaaagtac acaactatgc agcagtttga ctgcgaactg
     1981 gtttccttcc atcataagtt tgaatgactt agtgatggag gtacagaaag gttgttaact
     2041 ttggatgata ttaaaacaat aaaacatttg agaaataatc gtgatcagtc aattgatcag
     2101 ataagaaaag caatgaatat caattggcga acagcgaaaa aatacgccga tagtgatcaa
     2161 ctattcatta cagatcccac tcgaaaaaac aaatccatga tggatgatgg ttggggagaa
     2221 atcatcggta gttggctaga agaagaccta cgtttacgca gaaaacttcg tagaacggcc
     2281 aaagctatcc atgaacaatt gaaacctttg ggtttccctg gctcctatcg aacagtttgt
     2341 gtatacattt ctcaatggaa aaagcagcgt tacgacgaaa cgaattacca agctgtcgat
     2401 cgtcttgatc atccgatcgg agaagcacag gtcgattttg gaacaatgga agttatgcat
     2461 gaagatcaca tgaaagatat acaacttctt atcatgtcat ttccctatag taatgcgacg
     2521 tccgcaagtg ccatgccagc tcaaaaccaa gagtgtttcc tggaagcatt aaaacaacta
     2581 tttaatcaat gcggcggcgt tccaaaaacg atcagaatag ataacttaac tccagcggtg
     2641 aaaaaaacaa aatcaaaatt tgaagaagca caactaaccg atgaattctt acgtttcacc
     2701 attcattatg gttgtgatgt tcaagtctgt aacccacgta gtgggcatga aaaaggacat
     2761 gtcgaaaata aagtgggtta tgtccgatac aatttcttct cgacttcgcc aatcatggac
     2821 tcttttgagt ccctaaataa gcaacttgaa gaacaattaa aagcagatcg aaaaaggacg
     2881 cattattcaa aacatcaatt aatcgaagat ttgtggaaga aagaaaataa agcgttacat
     2941 tctcttccac aagaggatta cccaattttt aaagaagtgg aagctaaact aaatcgttat
     3001 cacgaaatga aattggatca aacggttatt catattccta aagcatatgg ctattctcaa
     3061 ttgactgttg ttttgacttg gaataaattc aaagttctta cgcctcatgg agaaatatta
     3121 atgaacgaat atcgccccta tatgaacaaa aaaagattga tcccatggag tgacatcatg
     3181 aaacaatgga gtaataaatt gaatgtcgtt agttattcga ggtattggaa gtacttgcct
     3241 gcacgccttc aaaattatct agcgattgat aataatcacc tgaaatacag acggataaaa
     3301 caattactaa gtttattagc gacctacgat atccaacaaa ttaatgaaca attttacgaa
     3361 ttagtcgttc aagaagacgc cccctctgac tttgatgtga attggtcaaa ctatgatgca
     3421 ttaacaaaaa caggaggtgt gcaaaaatga ttggacagac gttagaagat tattgtaaaa
     3481 agcttcgatt aagccatatc atcacgaaca taccaagagc ttctttcgaa gctccagaac
     3541 aatttattac cgatttattc cgtttagaac tggagaccag acagcagatc aaattagaac
     3601 ggatggaaaa attagccaaa tttcctgtac caaaatcgct aaagaactat gaatggcacg
     3661 aagatatttt tctaccatct catctagata aggagaccct tgtctctctt gattttctaa
     3721 aaaggcaaga aaacattgtt tgtgttggct ctcctggtac agggaaaaca catctagcga
     3781 tcgcattagg gagaaaagcc tgtagtgaag gtgtttcaac caaatttttc cgagttaaca
     3841 atctcgtaga gcaattagag aatgcattaa aaaaacaaca gctagccagt ttcaagaagc
     3901 gattcgaaac aacagaatta attattcttg atgaaatggg gtacttacca ttgacaaaag
     3961 aaggagctga acttctcttc caactgattt cagattggta tgagcaaaag agcattatta
     4021 ttacctctaa tttagaattc agtcaatgga atcgagtgtt tatggattct cgattaacag
     4081 ctgctctcgt tgatcggcta atccatcacg ctcacattat cagcttcaaa ggcgaaagtt
     4141 tcagattgaa aaattcatta tctaaatcat attgaaggaa aaaactagtt gcagcaaaac
     4201 tctgcacttt cggctgcatt tttttgtaaa aagctattgc acaacacaaa catttgaacg
     4261 acgtaattcc aaatgttgga ttgcttttag ataatctttt atttattcac aattgctaat
     4321 agtccaattt tcatttttgt atagttttaa tctatactga aaaacacttg ttgttttgga
     4381 caactcatca agatactcaa caccaacaat cacttgaatc gctttaccct tttgtttgta
     4441 aataggattc actaattcca caaatttcaa attagtatta attggttgca tcgcgtcttt
     4501 ttcaacataa tgctctagtt ctttttctga ggctgctgga taaagcctga aaaacgtctc
     4561 taaaaactcg gtgatttctt ccgttgtctt tgaatcaatt tttgaatcac tttttacttg
     4621 tcttacttta tgttttgcct tttctggctt accaatcata gtgggtaaac tcgttacaat
     4681 atgattgcct ttttcatctt caaagatcat cagacgatat gcagaagaaa ttgattgtgt
     4741 ctttttccct tctgtaatct tttgatctac cacaaatgaa acgacaaatt cttccgagtg
     4801 tttttcaaca tctagaattt ttactgatac aacctcggaa ctagtgggaa tatccgctcg
     4861 aaccatatct tggctcaaag cgagcccttc ttctgtaagg tactgctcaa gattagtcat
     4921 tctcttttca attacctcct tgttattctt ccatgaaaaa tattctttca caaaatctac
     4981 agtaaaattt tctactccag aggtattcac tattttctct ttcacgacca ctttctcatg
     5041 agttgtatgc tgatcaatgg ccgtgaagtt tttgtagatt ccaaaagcaa ggctacaaga
     5101 caataaaagc caaagggcaa gcaccatttt tcgatgcttg cccacggata ctactcgttc
     5161 tttcttcttt tttggaatct tttccttgtt acttcgctca atttttatct tcattttcca
     5221 ttcctctttt ctattgttta taccgaccag caccaataat gtgctgttgc caatagctat
     5281 cggtaagatt ggtccaaccc accggatctc ctgcatgaaa cattctgttc tctcctgcat
     5341 aaatgcccac atgagtcaca taagtaccgg catcataagt tgaatgaaag aaaatcaaat
     5401 cgcctggttt ggcatctttt aagtcaatat gagtcatagc atcatattga tcctgagcca
     5461 cccgagggag tttcaaacca actttggcat aacaccattg agttaaacca ctacaatcaa
     5521 acccagttcc gggacttgac ccaccaaata cataagccgt tccttcatac ttgaacgcct
     5581 cgtcaaaaat tccttggact gttttatcat caaactgtgt tgtagttagg tactgcttca
     5641 ctaataagca ataaaacata ttgccatagt tataccgcca cccactattt tccttaatcg
     5701 cgatgggatt gggataatcg actttttgac cacccgattt ttcttttgaa aaagattcgg
     5761 ctaaagcaaa actatgcttc tttccgtttt tcgcgacata atccaaatac ccgtcaccat
     5821 agttataagc ctgaatgata ctatcttgat cgcatcctag ttctttggaa cacttaacca
     5881 aagacgaaaa atagagacac acttgtttga tggatgcctc ggtacttaat gaattcggtg
     5941 ataaacctgc tgattcagaa ctttgcatga catcttccat tgtacctcca gattcgactt
     6001 gaataatcgc caaaagaatc gaaacatact ctgttatatc tgattctttg gcatactttt
     6061 ccaccattga acggtgtttc aaaactactt ctgagacact actacctcca ggagaagttc
     6121 cagaactacc acttccctca ccgtcctcac caaacaacaa acccacacac atcaagatgc
     6181 ccacaaaaga gatggtcagg aaggttaacg aaataatact caattttttt aagttcatct
     6241 tttctcacct ggctttggtt ttgggcgagt gttctttttt ctttgtactt gttgattggt
     6301 tcgctttgga aaagtgaatc tctgtttcac ttgttgaaag gaattgttct tgcttagtgg
     6361 ctgagtgctt ccgtttggaa tgacttcttg actagaaagt ttccgtttga tttcaggatt
     6421 cgaatgtact tgtacccttg gttttaaaga cctattttct ggtagttcat ttttctgtgg
     6481 ttttatctcc ttttttatcg gaacattttc ccttcctttc tcatgacgat tccatgcttc
     6541 gttcaatact ttgcgttttt catccatttt tgcgtctctt cgttgtcgtt gtttttctcg
     6601 caactcccct cgatttgctt tttcttcgaa aggccctcgc ttaaagtcct ctggtacttt
     6661 tttcgtcttt tccatgcctt tatgaaggct gtatttcaag ttagttggtg catcggttac
     6721 ttgatttttt gcttgtttcg ctttgtcctt cacgatttct tttgtatcga gtacttttcc
     6781 aacttttcta ccaactaaat ttattcgtga ttctttcttc gtgcccactg gttgttcttg
     6841 ttttttcaga tcagaattta gctcgttaat gcttctcgcg gaatggttct ttctattgga
     6901 cactctattg atttttgaac tacctttccc ttttgtagaa ctggtagcta gtttcccacc
     6961 aactgctgca ccgccaccaa aagcaaaagt tcgacccaca tttcgattaa gctgtcgcaa
     7021 ttttcgattc atcaacattt gcggtttccg catgactcgc cttcccattg actgactatc
     7081 attactttga aggttaaaca tgctcataat gtcacctaat ttgaaataga taccggcaaa
     7141 ggtcacaatt tgtagaaaag ctaccataaa gaagggatag gtggccgaaa tattgaaaaa
     7201 catcgtggag atactgaatg cagtcgtaat gactaaggta actcccgctc gcatcataat
     7261 cgtattgaac aaacgaataa tcgccctttt gcccaaactc tcatacgtcg gcaacatcga
     7321 caataagaaa ctaatgggca gaaacatcgc aaagataatg aaaaggattt gtgagaacaa
     7381 cataatccct gtcagaagaa acacaaaaaa ggaaatccca atattaaaca acactaaaaa
     7441 gaccacggtt cctaaacggt tcatggtctt ggtaatactg agattcgcat tgtcattatc
     7501 ttcaatctct gctttgactg cttcttcccg atccttaccc ttattttcat ccggactcac
     7561 ggataaaatc ttattaaccc gaccctcgcc aatttcttct atattggaat catcgaattg
     7621 taatagtaac cagggttgtt gaacttgaat cgaaaataaa ctgtctcgaa tcaaatccac
     7681 actatctttt ccctggcttt ctgaattagg tacaacaatc ttggttccaa ggctaagagc
     7741 cgcttcacta acgtctgagc taaagtcatt gatttttgta atataggtgg gcgcatacgc
     7801 aatgaaagaa cccgagagta gaaaaatcat cacaaaatta atgacggttc ggactgcttt
     7861 ggtagtttct cgttttaata atccggtata cgccacataa actcctagag ctaaaatcat
     7921 caataatagg aacccaaagt agaatcccga agtctgtaat ccattttcag tgataccggc
     7981 taaagtttgg atattctttc caatactttt agccgtatcc gaaatgaaat ccaacttgta
     8041 agcttcttga accacatagc ccgtggcgtt tgataaatac agacttactg tccaaataaa
     8101 attggtaatg gcataaagac catacatcac gcttttccct aagccatccc cccaattcca
     8161 aggtaaccaa tcccaagaac tatccacaaa gaaatccagt tgataattgt tcagagaata
     8221 tttggaataa aggtttccgg cttcaactgt atcatcaacc agacctgtcg cctcagctac
     8281 cgtaccgact aaactcaata gaactgtcaa taggacgatg ctaataaaaa cgattcccac
     8341 aatccgaaaa agtttctttt tcatcccctc actccattct ctcttgaacc ggcggacgag
     8401 tgtcaaaagc atggaagagt tcttcaaaga ctggatgaac ttgaaccaca cctactcgcc
     8461 catacaagtc ttgcatcaag cattgaccgt ttt
//
LOCUS       DAJSVF010000110         7656 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSVF010000110 DAJSVF010000000
VERSION     DAJSVF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.113"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            22..2349
                     /locus_tag="ON550_002527"
     CDS             22..2349
                     /locus_tag="ON550_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3955554.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2475..2804
                     /locus_tag="ON550_002528"
     CDS             2475..2804
                     /locus_tag="ON550_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3955555.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2844..3848
                     /locus_tag="ON550_002529"
     CDS             2844..3848
                     /locus_tag="ON550_002529"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3955556.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3845..4291
                     /locus_tag="ON550_002530"
     CDS             3845..4291
                     /locus_tag="ON550_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3955557.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4441..5256
                     /locus_tag="ON550_002531"
     CDS             4441..5256
                     /locus_tag="ON550_002531"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3955558.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5462..6634
                     /locus_tag="ON550_002532"
     CDS             5462..6634
                     /locus_tag="ON550_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3955559.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIY
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6796..7290)
                     /locus_tag="ON550_002533"
     CDS             complement(6796..7290)
                     /locus_tag="ON550_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955560.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7287..7481)
                     /locus_tag="ON550_002534"
     CDS             complement(7287..7481)
                     /locus_tag="ON550_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955561.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2574 a         1445 c         1526 g         2111 t
ORIGIN      
        1 gattttttat tgagaggagg gatgaaaatg attaagaaaa ctcaaaataa aatccaacgt
       61 atatgtcaaa aacattttgt ttctagactc aaagctgtat ctaaaagaaa atacttacat
      121 cccattatga tttcgatcat cttgattttt actttcttag ggagcttatc aagtatttat
      181 gtaaacgttt atacagaaac gtcaaatttt gctaaccaaa cgacagagga gacaaatagt
      241 ccaccctatt ctgaaataca gtccatagaa agcaccaatc agtctagcaa caacgagaca
      301 acctcaacca catcctcgac tcaactgatt gatccgacag aaaaagaaat cacagcagca
      361 aaagcagcag gggagaaagc ttattcagaa acaggtagaa gccagacatt tagtactgtt
      421 gcggccactg cgaatttcaa cggacatacc ataccagatg gatatacagt gatatatgcc
      481 aaagatggag tcattgttgc ggttaacgga tcgatgagtg cggataaaac tgcgttacaa
      541 ggcgtaacca aagcaatcgc ttttattgat aaccgtccag atatccataa tctgtttatt
      601 ggctccacag taaatggtac tactatcaca ttcccaaaaa gtatctatat gggggtggct
      661 accgctggaa atacgatcga ttcagttaca cccaatgcgg ataaaagtta ttcttttaca
      721 ccctcaacat ggggagggct tcctgccgga ggaggtatga gtactttttc aatcggtata
      781 cagttagcaa atgatattcc attcacgggg tactcgatca aaaatgggca gatcgttgat
      841 ggtaagggaa atgtttcaaa ttttccaacc tatcaagcag caacggttca ggtcaatgcg
      901 aatcggacag gaactttttc ttatacagtc aatggtggag cagcaaaaat cggaacgtac
      961 gggactacat tatctggaat ccatgtgggt gacaaagtgg ttatcactcc aacagcagta
     1021 acgggctatt cttttgcaca aacaaacggt tctttcacag ccctagaaag taacgtcgat
     1081 acggtgacct atacaggcaa acaaacacca atcactatta agtatcaaga ctattttgga
     1141 cagatgattg ctccttctaa aataatgaat cttacttacg gaagtgctgc acaagacctg
     1201 acgactaata cgcctactat caacggatat acctttacag ctgtttcagc gatagaaaca
     1261 aagaaccaat cagcaatgag tgttttagct agtttagata caaatggaaa tgtaatcgta
     1321 aaggacgcaa atggaaaaca gatttctgaa gtcatcctat attataagac aaatgcaaca
     1381 gtatcaatca atgcaaatgg cagtaagtac tatgatggtc tttctgttgt accgatcgtt
     1441 aaatatgcct ttaatgataa aacagaatca acatcccttt taaacaatga tctcagtatt
     1501 gatcaaatca catggaatcc aactgatttc aaagcgatga atgaaaaggg agttgaagta
     1561 actgcgccta ctgaaattgg aacctatacg tcttggcaat tgacaaaaag cggactagct
     1621 aaactagctg ccagaacaaa ctatttattt actatcgtgc agacatctga tgtctatacg
     1681 attaagcaaa tcagcggtgc ggtagagctc agtggcagta agacatatga tggaaaagca
     1741 ggtgtcccgt caatccatgt caagctagca gagggtgtca ctagttcgct tactccagta
     1801 caagtggcac ttagcagtat ggattacaca gcagatgcac aatcagcaaa aaatatggtt
     1861 aatgctggca gctatacaat taaactaacg aatagcggaa tagacaaagt caaagcagca
     1921 aatcccaagt tgtcatttac tgatatagcc aacacatcag gaacttatac catctataaa
     1981 gctaacgcag tgattactgt cgatgacgct gcctttaatt atgatgccca aagtcactcg
     2041 atttcggttg gaaatctcca cgttaccggt gttgtcttag gcgaatcatt ggattatacc
     2101 ctaacagtta acagccggac agatattgga tcacaaatag taacgattag tttaggcgaa
     2161 ggaagtgtaa acaataatta tacgattatc gcaacagata ccgctaagtt gacgatcaat
     2221 ccgactctct tgcttccttc gacaggtggg ataggtctac tgccctttgt ctttttagga
     2281 ctgatcttta ttttttctgg cttcttttat tttatccatc gtaagaaaaa agcaggtgaa
     2341 cagcgatgaa aaaaacaaaa aatgtacgtg tggatatatt tgctgttctc ttttttatcc
     2401 tactggtctt ttttataggt tctccgactg tgtcagctag agaatcacct attacattta
     2461 ctttgcataa tcgaatgttc gaaagcgata agatgccaag tatcagtcaa aacgacggaa
     2521 taaatatccc ggataacttt caaggagtaa aagggttgaa cggcgttact ttcgctatat
     2581 atgatgtctc ggatgaattt tacaagctgc ggtcagaagg aagtagtgtg gaagacgcac
     2641 aaagaaaact agcacaaaaa agtgatagtg aaaaaatact ggcagaaaat gtgacaaaaa
     2701 cagtagacga ggaagagggc attgcttctt ttagtgcatc ggacaaagat gagcaaggtc
     2761 gtgacgctgt ttaccgattt acggaaataa agacttcaga ttaagtaaaa gaaaaatcag
     2821 caccatttgt cgttgtgctt cctgtgacag attcatctaa tcataaattg acgaccatcc
     2881 acttgtatcc aaagagcgaa caaaaaatac ctgctgcact cacattaaca aaaacagtag
     2941 agaataagca gacagatttt gcagatgggg ataaagtccc ttatctcatc accgtaacaa
     3001 ttcctgagaa catcaacgag ataggaacct atacgatcaa agatacagct gatcctcagt
     3061 tatggctaga accagaaacg atcgattttt tgattggtgg agacaagatt catacatttc
     3121 acacacaaaa aataaaacat ggttttgtct tatcagattc tggaaaagat cttagttcgt
     3181 ttgctggaaa aaaattaacg atccgttacc aaatgacatt gaaagaaaac agtgaaaaaa
     3241 cgacatttga taacgatgta cgtttgacga ctgacaatca aacagtagaa acacatcttg
     3301 ccatccaaac aggaggcaaa caatttgtca aagtggatcg gcaaacgaaa caaaaactag
     3361 cagatgctca atttctagtc aaaaaaaatg accaatactt agcgcaagaa aacgggataa
     3421 atcattgggt agccaaagat gattcaagag caacgatttt tacttccagt aaagatggaa
     3481 cattttctgt ccatggattg tcttttggaa gttacgaact agtggagatt aaaccgccta
     3541 aaggatatat attgagtcaa tcaaccattc tctttgaagt tgaaaaaggc agttatgatc
     3601 cgcttcatcc tataccattg gaaatcatca acgaaccaaa agcaaatccg acgaataatg
     3661 gaaaaacgcc agttcagatg cagacaaatg gtccatcaaa tgaaaaaaat ggaggaagct
     3721 ttccaaaaac aagcgaagag atgcttggag gtttctcaat tttagggtta ttgctagtcc
     3781 tttcaacagg gacagcttgg ttttataaaa aacaacaaaa aggaaacaat aggagggaaa
     3841 taaaatgaaa aacatcaaaa caaaaatatg gacgttttta ggaactgcta tcatgctgct
     3901 tcctttcgtt ctcggcctag gaactgcaga ggtttctgca gccgtatcac ctacaccaga
     3961 aaatgttacg gttaatttgc ataaattgaa attcacttca gcaccagaaa atcaaataaa
     4021 caacggaacg gaattgacat ttccgaatag cgaaccgttg aacggcgttg aattcaacgt
     4081 atatgacatc actgctacgt actatccaag taaagacaca gcagttccag cagacgctac
     4141 acccttcgca agtgtaacga cttctggtga aggattagct aacctgactc ttccaggtaa
     4201 atctgatggg aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac
     4261 gtcgccaaat atcgtgttaa gcttacccta gaccctgaca aacctttaga cacgattcac
     4321 ctatatgcta aaaatgtcat caaagaaagc aatatcaaaa tccagaaagc tgggaacttt
     4381 tcaggggtag ataagttagc aggggcgcaa ttccagatca aaaattcaga gaatcaattc
     4441 gtgaccggtt ttgatgaaaa cactggtgtc gctctctatg atcctgctga gtcagcgttg
     4501 acattcacaa ctgatactga aggatccatc agtgtcaatg gattaataga cggcaattat
     4561 actttagttg aaacaaaagc accagatggt tatcaattaa ccaatccaca aaaagaaact
     4621 acttttatag taaaaaacgg aaaattgcaa caaacaaccg gtatcatcca tggcgcagat
     4681 gaagccaatc ttgggaatcc aaacggtgta gtcaatcggg aaaatacaac tacggataac
     4741 acgattactg tagaaaatgt ccaaaactac ggtgatttcc acttcatcaa acaagatgtg
     4801 aacacgcaag aaaaattagc cgatgcagaa ttttctattc taagagcaaa agatccaact
     4861 gttaaattaa tgaaaaaaat agcaaacctt gcctcaggag aatacccata tgcttggtca
     4921 gatgaagtca caggttctga ctgggaagaa gtgaaattga tttctgattc agaagggaaa
     4981 ttttcaatcg aagggctgaa acaaggggca tactgccttc aagaaacaaa agcaccagaa
     5041 ggctacgcat tacctaagaa tgaagctgcg tacactgaat ttacagtaga tgatgatcca
     5101 acaacttctg atgaaacaac gatcgataac caacctaaag gaatcttgcc ttcaactggt
     5161 ggtatgggga tcatcgtatt catcttggta ggtactgctt tagttggagg agcagtgatc
     5221 tatttcaaaa aacgccatca agaaacagaa gcttaaacta ttggaaacaa aaatcatacg
     5281 ataaataaac gatagtgatt caaatgggga gaaaggttgt gacaagtggt cggtctcttg
     5341 gtattcgctc aacaaacggc aaaataggag gaactatttt gccgtttgct tcgctaatac
     5401 tcaggtcaag gcgctgattt cacaacctct tcatatacat attagaaaat aggtggacat
     5461 gatgaataaa agcagaataa gaatactgga catcttcatg gcaatcattt tagtcgttgg
     5521 gatcgggatc ttttcttatc cttttgtaga agattcactg aatgattttc ttgcccagca
     5581 aatgatcatc tattatcaaa agcaagcatc gaataagaat tcagaggaaa taaaaaaaca
     5641 gcaggaaaaa atgacaaaga aaaaccaaca gttggcagag caaaacgtat caccagggat
     5701 aggcagtttc aaccaagcag ttgatgcaaa agcattaaaa gatttaccta gtaatgcctt
     5761 ttttatggaa catatgcttg gagtcatcga aatcccaaaa atcaatgtca gcttacctat
     5821 attcgatcag acaacggaaa tatttttgca aaaaggaacg tcattattgg aagaaagttc
     5881 ttatccgact ggcgggaaaa acacccatgc cgttctttct gggcatcgtg gactcccaga
     5941 agcaaaatta tttacagatt tgcctaagtt gaaaaaagga gaccagtttt tcatccagat
     6001 caatggaaag acattggcgt atcaagtaga aaaaatccaa gtggtcttac ctgatgaagt
     6061 agattctttg ggtatccaaa aaggcagaga tctcgtgact ttgcttacct gtactcctta
     6121 tatggtcaat acacatcgac tgcttgtgac ggggcatcgt attccttatc aagcaaaaga
     6181 ggcaaaaaaa gcgatccaag gaattgatca atggaaaaaa tggaaatttt ttatttggtt
     6241 tatcggtata ctacttggat caatcggctt agtttggcta ctcatcgctt atttagattc
     6301 tttggccatc gctaagcgaa actatccttt atctttttat gtaaagaaca caaacggacg
     6361 accaatcgaa ggaatggtct tttctgtaaa aacgctcaac ggcaaacact atattactag
     6421 agaaaaagtt ccatttgtga aagcttctga tgaatacgga ctagtgagat tttctgattt
     6481 aaaaggcagg aactatcgct tgcaacatga ggagctcctg cttaaaatcc atgtaaaaca
     6541 taaacattcg aaacagtttt caatgaaatt gaaaaaagga agatataaac tgcgaaaaga
     6601 aaaagaggtg tactatttga ttgagaaaga atgaatgaag atgtccaaaa aagcagaggg
     6661 aacagactaa atgaaaaaag agagaagctg acgaaatgga cgtgtaaact gttatttttg
     6721 gtttactatc tggaacgcca tcttctattt ttttatgttt cagaagaatt ttattattat
     6781 agtataactt ttgaattagt ttccataaca tttggagcaa ggggttaaac cacgagcttg
     6841 agcttcgctt aaagtggcag gggtatacgt accatttcca catttatgtg tatggtattt
     6901 tgaaccggtc ggtgtgacat agacagtttg ttcattattt tgctcaggag ctgcggcttg
     6961 tgcctgctga gaggcctgtt cagctgctga ttgctgtcgc gcctgttctg cttgttgctg
     7021 tgcgattgct tcttgcctcg cctgttctgc ttgttgctgt gcgattgctt cttgttgcgc
     7081 ttgttctgct tgttgtcgtg caatttcttc ttgctttttc cgttcttctt ctgcttcttt
     7141 tgctttgatt gtggaatcaa ttgcagcaag acggcttgtc aactcttgtt tgttttcagg
     7201 tagcgcttgg atagctgctg aagcagttgc atagttttct tttgtaggat ttgcttcagc
     7261 ttgtgacaaa gcagattccg cttctgtcac tttttttcta gtgcagcttt ttccgctttt
     7321 tgtttctctt cttttttctt ttcaatttgt tcttgttctt tcgatgaatc tgccgtactt
     7381 tcggagatcg tggtactgct gctggaagcc tgatctgaac ttgtgctttg agtagtagac
     7441 gtagtagcac cacacccact caacaaactt cctgcgacca aaaacaatgc aactaatttt
     7501 ttcgctaaat ttcctcctta agtaaaaaac aaccgttttc aataaatgtc cttttcaatt
     7561 gtttacttac ttgttataat aattgtgtta tgaatagtga ttgaggaaac ttaatctaat
     7621 cccgtgttga tacacgggat tttttgctat agttct
//
LOCUS       DAJSVF010000111         7630 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSVF010000111 DAJSVF010000000
VERSION     DAJSVF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.114"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(16..708)
                     /locus_tag="ON550_002535"
     CDS             complement(16..708)
                     /locus_tag="ON550_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3955562.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1145..1714)
                     /locus_tag="ON550_002536"
     CDS             complement(1145..1714)
                     /locus_tag="ON550_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3955563.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="ON550_002537"
     CDS             complement(1788..2111)
                     /locus_tag="ON550_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955564.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="ON550_002538"
     CDS             complement(2265..3077)
                     /locus_tag="ON550_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955565.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="ON550_002539"
     CDS             3431..4279
                     /locus_tag="ON550_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR3955566.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="ON550_002540"
     CDS             4424..5365
                     /locus_tag="ON550_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR3955567.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="ON550_002541"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="ON550_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2273 a         1512 c         1372 g         2473 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctttt
//
LOCUS       DAJSVF010000112         7514 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSVF010000112 DAJSVF010000000
VERSION     DAJSVF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.115"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            369..878
                     /locus_tag="ON550_002542"
     CDS             369..878
                     /locus_tag="ON550_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR3955568.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            978..1628
                     /locus_tag="ON550_002543"
     CDS             978..1628
                     /locus_tag="ON550_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955569.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            2079..3017
                     /locus_tag="ON550_002544"
     CDS             2079..3017
                     /locus_tag="ON550_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3955570.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            3030..4082
                     /locus_tag="ON550_002545"
     CDS             3030..4082
                     /locus_tag="ON550_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3955571.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            4348..4983
                     /locus_tag="ON550_002546"
     CDS             4348..4983
                     /locus_tag="ON550_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3955572.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            5216..5620
                     /locus_tag="ON550_002547"
     CDS             5216..5620
                     /locus_tag="ON550_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3955573.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            5753..6796
                     /locus_tag="ON550_002548"
     CDS             5753..6796
                     /locus_tag="ON550_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955574.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            6811..7374
                     /locus_tag="ON550_002549"
     CDS             6811..7374
                     /locus_tag="ON550_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3955575.1"
                     /translation="MITVVHKERQEKSSSDVQLWVYLGSISTKIQYGYTDSAKKKGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
BASE COUNT         2471 a         1149 c         1561 g         2333 t
ORIGIN      
        1 cggcgaaaaa aataggcata ttaagcgggc taggaggaga aatattcaca tatcattttt
       61 tcattccgca agctgcgtca agttatttgg aatttgtaga acagatgaga gaggtagaag
      121 cagcactaca aactactttt caataagtag gaattataga aattttactc aaaaaatctt
      181 cattaaaaaa tctgactaaa taagttgaga aaacagaata tacttcaata tccagtgatt
      241 tataaatatc attcactcat tacccagcgc cttatcataa gtgatgcggt ttttatcatt
      301 gatcctaaaa atcttagcaa gacaaaaagt acgtaagaaa gtaaagacta aatacttcgg
      361 aggaacaaat gaataacaat gataggttat tgcgattgcg ttatgcgatt gatttgaaag
      421 atacggattt gattaaagct tttgagctag gcggtgtacc actgactaaa gaagaagctc
      481 aggcggtact gaccaaagta caggataaga acaaagataa tgcagaaaat aatgtgtatg
      541 aaaaaaccat caacaaccaa gtttttgacg cattcctaaa tggcctgatt ctcttggcgc
      601 gtggtccgca aaaagataag ccgatagtag acacgccgca aaaaagcaag gaaatcaaat
      661 atatcaacaa tgtgttgcta aaaaaattaa aaatcgcctt gtctatgacc actgatgata
      721 ttttagacgt ttttgctgag gcagaaatct atccatcaaa aggcgaaatc ggggcatttt
      781 tgagaaaaga aggccaacga aattttaagc cttgcggaga taaatatatg cgtaattttc
      841 tcaaggggtt aggaatttat aatcggagaa aagtataaag aaatttcatg agaaatcaaa
      901 acaatggttg ttttgatgag agctagtttt tgtatgagat taaaatatta tcttaatcga
      961 ggagctggat tggttttatg aatgatgaat ataagaatga tgaagataag atgctttttg
     1021 aagagataga gaatcgatgt aggctcaatt ttgagcttcg gggaaagatg tctttaatac
     1081 aacaaaaaaa gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa
     1141 tctcggattg gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg
     1201 acatgaaaaa acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag
     1261 ctaaagggca agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg
     1321 taacacatag agctgatgga atggtcatta ctgtggggaa atcttcttct aatgatattt
     1381 ttttagatgg agatcttttt tatcaactca atataaatca cttgtctggc actgaaaata
     1441 taattttaag gacggaatat ggcaatgaaa tatttgcaaa atatgatgag attttaaaaa
     1501 attatttaga ctgggcttgg ataattccgg ttgagtcagg agatgctaag aaactggaga
     1561 gattattggg cgatgaacta attaataaaa aagtaccaat tttaaattat tattcccata
     1621 gacaataaaa ggctattttt catgataata ctagttataa ctgtaaaaat ggaagaaaat
     1681 ctgggaaaga agtttttaaa taagtgtatt gttcgatttt aataaataat aagcaatacc
     1741 atccgtctgt gttgcgcaac atcgtactac aatgaatatt ttttcacaaa aagaaaatca
     1801 tccatcgaat gaatggaagg ttttagtata cacgaggtta atgccccctc ttttataatc
     1861 agaggagtga gattttttct atgaatgttt ctattttctt attgctagtc tagtttctct
     1921 tacgatgaaa ttgggattct attccagttt ttattgattt gctgataaaa taacttgaat
     1981 ggtggatata aacatgataa ataagagaag aataacaaaa cgaggttgtg aaaagattct
     2041 caaatgagta taatagaaag aaaaaggagg aagaatcaat gaaaatagca attgcaggcg
     2101 ctggcgcaat gggaagtcgc tttgggttga tgcttcacca aagtgggaac gaagtattat
     2161 taatcgatgg ctgggcagaa catgtccaac aaatcaaaga acatggatta caagcaaatt
     2221 ttaatggtaa agaggtagaa gcaaaactac caatcgttct tcaatccgaa gtagaaaaag
     2281 aagatcaagt tgatctgatt attctattta ccaaagcgat gcagctggaa aaaatgctgc
     2341 aggatatcca atcattaatc aaaaaagata cagaggtctt atgtctatta aatggtatcg
     2401 ggcatgaaga tattattgag aaatttgtac caatggaaaa tatctatatt ggaaatacca
     2461 tgtggacggc aggtcttgaa ggtcctggtc aggtcaaatt atttggaagt ggttcagtag
     2521 aattacaaaa tttaggtgat ggaaaagaag cagctgcaaa aaaattagca gacaaattgt
     2581 ctgaatcagg attaaatgct catttttctg acaatattca ctattctatt tatcgcaaag
     2641 catgtgtgaa cggaacaatg aacggactat gcacaatctt agacgttaat atggctgagc
     2701 taggaaaaac atcgactgct cataaaatgg tggcgacgat tgtcaatgag tttgccaaag
     2761 tagcagcagt agagaagatt gaactagatg tcccagaagt cattgcacat tgtgaatctt
     2821 gttttgaccc ggaaacaatt gggttacatt atccttcaat gtatcaagac ttgattaaga
     2881 accatcgatt aacagaaatc gattatatca atggcgcaat ttctagaaaa gggaagaaat
     2941 atggtgttgc gacgccttat tgtgatttct tgactgagct tgtccatgca aaagaagata
     3001 gtttgaacgt aaaataaagg agagattgaa tggaaacttc acaaaaaata tcaccgaaaa
     3061 tttttctgaa taaagtcctc gctggtacag caacaggaat cattattgga ttgattccta
     3121 atgcagtatt aggtgccatt ttaaaatatt ttagtgcgta tccctttgcg gtaacgatcg
     3181 cacagttagc agttattttt caactagcaa cacctttgat cattggtggt ttgattgcct
     3241 tacaatttgg atttgatccg atgagaatga tggttgtagc cggagcttct tttgttggtt
     3301 ctggtgtagt caaattcaca cctgatttgg gtgaaacggg tgtttacgtt ggtgctggta
     3361 caggcgatat tattaatacg atgctgacag catcgattgc tgtcgggctg attttactga
     3421 ttggaaatag ttttgggtca gtgtcgattg tgttaacacc gattgttgta ggtgtaggtg
     3481 caggtttact tggtttttat ttattccctt ttgtaacagc aattacttct gcaattggat
     3541 cattgattaa taacttcact accttacaac cgctgttaat gtctatcttg attggttgtt
     3601 cctttgcctt tttgattatc tctcctattt caacagtagc aatcggaatg gcaatccaac
     3661 tagatggtgt ttctgctggt gcggctgcga tgggcgtagc tgcaaccact tttgttttag
     3721 tagtgaactc ttggaaggtt aataagtcag gtgtgacaat tgcgattgct ttaggcgcta
     3781 tgaagatgat gatgcctaat ctatttagaa aacctgtgat tcttattcct tgtttagtta
     3841 gtgcagtgat tacagctatt cctgtcgctc tattttctat ttcaggtacg cctgcttctg
     3901 ctggtttcgg cgtggtcgga cttgttggtc cgttggcttc tttagatgca gggctaaatg
     3961 gctttcttgt tgttctttgt tggttagtca tccctgtttt tgcggcacta attactcaat
     4021 ttttatgcga gaaagtattt aaactgtatg atcgagaaga agtcttcaaa tttttaggct
     4081 agtagatttt actaagaaat tttcaatcaa tggatgcgta agtgagatgt caacaacatc
     4141 tgacttacgt ttttttgcct ttttgagtag gagattgatg ctgacaactg aactcttcag
     4201 tgccattcga ttttttaaaa tgtagaagaa actcaccttt atgttattga ttgttcaaaa
     4261 ggcaactaga ataaaaatat gtgttaaaat gagaaaaaat ttgattgttg gatgtatcga
     4321 ataatcacta aaaagtaggg gaagaaaatg atttttttgc aaaaatatgg tttttatttt
     4381 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
     4441 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
     4501 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
     4561 ttgtatcacc tatttgttgg aacatcaaaa acacttgcaa tacttgttgt tgcaacagtt
     4621 ggtttatatg ggataggtga ttgtattttt actggcttat tcagtatcaa tacgaatgaa
     4681 agctcttgga atctatcgac ttggatccat aatataggtt ctgggctagg ctatgcaggc
     4741 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaagtggtca aggtacgctt
     4801 agccattttt atcttgtttt aacagttatt agtttactaa ttgcaggtct ttacggatta
     4861 gcacggattc cgtcgattag tcagtttgcc ttctttaagc aattgggctt ctttcaacga
     4921 ttaagttttt tctttaacta tttgccgatg atttgtttaa gtttttttca actacgaaag
     4981 tagaaaaagc agtgtgaagc tgattaagtg gttctatgat atttggtgtt gttactcaag
     5041 gcgagtaagg acaccaaata tcatagaacc cttctttttt tgtatagata aactgcttct
     5101 atcactgatc atcgatgttg tctaactgat gtcgccgaat gcaagcatta cctttgtcag
     5161 attaaaaaat actcgttaca cttaatatag aacgacgaaa gaaaggatga tctcaatgaa
     5221 aaaaatgtac gaaacagcaa tgatcaatcg tggaggccgt gatggcgaag ttgaggcacc
     5281 aaacggaagt atgcacatga agatcgaccg cccaggaatc catagtgaag ggacaaatcc
     5341 ggaacaactt tttgcggctg gatacgcatc ttgcttcaac ggcgcggttc aacacatgct
     5401 ggaagaaaat aatcttgagt ctgattctga agtcaaagca cgggtatctt tatttcaatt
     5461 agaagatggg ggctaccaaa tcggggtcgt tttagaagtt tctttgccag gacttgagaa
     5521 ggaccaagca gagaaaatcg cagaagaagc ccacgcattt tgcccatact ctaaagcaac
     5581 aagaggcaat attgatgtgg aagtgactat agtagaataa gggattctta atgttactat
     5641 aaatgagatt atgaaagatc ctataaagaa acgctagtaa aagaaacatg tagttacggt
     5701 aattaatagc ggtatatgga cgattgatcg aatagaacaa ggaggcaaaa aaatgacaca
     5761 gcgggtagca ataatgattt tagtgttgct ggtcattggc ctccttgttt actatgtcct
     5821 aaagtttaag cattggaagc agcaacggat acatcaggat attgaaaaaa agttaaaacg
     5881 gtatcctatt gttcaagcag cttgggaaaa ggctgaagca aaacaatata acattcctgg
     5941 attaacagaa acaagaatgg tggtgccgga aaccggcgaa aatgaggttt gtcaatggat
     6001 gacgccgcaa gggttggctt tttctcaaga ttttgtgttt atttctgcct actgctatga
     6061 ccatcagcat cattctatta ttcatgtact ggatagagaa acaggacagc cgatcaaact
     6121 gctgatttta cctaaacggc cacatgtagg gggcttagta tatgatacaa aacgggaact
     6181 tttatggttg acaatcacag gatctgctac tggacgagta gccgctttac gtttgatcga
     6241 tatcttagca gatacatctg aagagacggg acagccaatc gcttattggc tgacaactga
     6301 tctctcagag attccacaag catcgtattt gactcaaaac aatgatcaac tggtatctgg
     6361 caacttcaca ctaaaaggtg aaggacagtt gactttctat ctattgccga cgatagctga
     6421 aatgaaaaca gcaattcgcc gcaaagacaa aatccaacca accgtgacaa tgagcagaaa
     6481 aacatctgat aagattcaag gtgtggcttt ttatggccat tatctcttag ccagtcgttc
     6541 ttatggtcct tatacctcgg aactcttagt gtccgagcgg gactctccta gccggatcct
     6601 aaaacgaatc aaatttccac cgtatttgga acaaatcgtt gttgtagaag atcgtctagc
     6661 agtattgttt gaaagtggcg ccgcagcgta tcgcgagaaa gccaatcctg tgttagccaa
     6721 tgttttatta ctagacttag caacgttgct tcatgcaaat aagcgtccca aaaagattgc
     6781 tgcaactaaa aaatagtcgg acaactcaag atgatcactg ttgttcataa agaacgacaa
     6841 gaaaaatcat ctagcgatgt tcaactatgg gtttatttag gaagtatttc tactaagatt
     6901 caatatggct atacagattc ggcgaaaaaa aaaggcaatg ttaagttttt aagaatcaca
     6961 gatatacaag agggacgtgt aaattggtcc agtgtgcctt actgtgatat tagtaattct
     7021 aaactagttg atttaagatt ggaagaaaat gacattttga tagcaaggac aggaggaact
     7081 atgggaaaaa gtttcttggt taaggaaata agtgaggaat ctgtttttgc ttcttacttg
     7141 attcgaatta ggttagttga gaaattatta tcagaatacg tagactgctt cttagactcg
     7201 ccattatatt ggaagttatt agaaaaaatt tcttacggaa ctgggcaacc aaatgtaaat
     7261 ggtacaaatt tatctaaact acttatacca ttaccacctt tagaagaaca acaacgcatg
     7321 actactaaaa tagaaatgat aagaagatct attcgtagaa tcaattttga gtaaatataa
     7381 cggacctagc atccatttta taccagatga tgctaggtct attatttata ttttatggaa
     7441 tttaattgat ttgttctagt gttttaatat tattacgaca gtaaaaaaat agtttgattc
     7501 ttctaacatg aata
//
LOCUS       DAJSVF010000113         6937 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSVF010000113 DAJSVF010000000
VERSION     DAJSVF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.118"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(6..266)
                     /locus_tag="ON550_002550"
     CDS             complement(6..266)
                     /locus_tag="ON550_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955576.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            complement(632..1117)
                     /gene="ssb"
                     /locus_tag="ON550_002551"
     CDS             complement(632..1117)
                     /gene="ssb"
                     /locus_tag="ON550_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3955577.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVGADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1120..2019)
                     /locus_tag="ON550_002552"
     CDS             complement(1120..2019)
                     /locus_tag="ON550_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3955578.1"
                     /translation="MTPTQFKAKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALMYEHKLMFENKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            complement(2035..2628)
                     /locus_tag="ON550_002553"
     CDS             complement(2035..2628)
                     /locus_tag="ON550_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3955579.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILIYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRDTNKLRRYMDILKSPKEMTSYMLPLY"
     gene            complement(2702..2851)
                     /locus_tag="ON550_002554"
     CDS             complement(2702..2851)
                     /locus_tag="ON550_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955580.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRVSKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(2867..5071)
                     /gene="topB"
                     /locus_tag="ON550_002555"
     CDS             complement(2867..5071)
                     /gene="topB"
                     /locus_tag="ON550_002555"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3955581.1"
                     /translation="MKTVILAEKPSQAKSYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEYLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFVAKLDPNETQARQEAFEDYLKEKGVQLGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVIKGETVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKELI
                     QNGRTSQTVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            complement(5082..6542)
                     /locus_tag="ON550_002556"
     CDS             complement(5082..6542)
                     /locus_tag="ON550_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3955582.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLLAELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LRIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIGSINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(6603..>6937)
                     /locus_tag="ON550_002557"
     CDS             complement(6603..>6937)
                     /locus_tag="ON550_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955583.1"
                     /translation="QEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDA
                     AFIEDPRHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQ
                     QLSQKERV"
BASE COUNT         1775 a         1445 c         1175 g         2542 t
ORIGIN      
        1 tttatttata tgcccatcga tcgtgtaaaa tgatcgggat ttctaacata catgctagca
       61 ataaccagct gatttcgttt attgaagtga cctgtgacgg caatattgta ttttccatca
      121 tccataatta gcaatttatc gataatttca atatctgcaa ctacacaatt aatcgattca
      181 tgaatcgttt gtaaagtaaa gcgaaccaat aacggttgcg tttttattac tcggatttta
      241 tcaagcaaac caacatattt gttcatccca atcacctcgc taaaattata gcaaaaatca
      301 aatcatttgg atatttcaat tatgctataa tagtcttgct gtctgctacc ccggcctaat
      361 ctggcaaggg aaggaggtta taggtttgaa aattcttttt gattttgttt ttgcaatcat
      421 ggcagaagta gccgcgcact atatctgcaa atggcttgat tgtaaatgtg ccaagaaaga
      481 cagtaagcct acccgttaat tcttataata gaaacgtaaa agaaagccac tagcccgcca
      541 gctagtggtt tttggttata gattgaaaat tctttttgtt gtctaaatta taacataaac
      601 cgtagcaaat gcaaccaata gaaaatgcta cttagaacgg tagatcatca tcgctaatat
      661 cgattgattg tccaccaaat ggattctgtg aagcgttcaa tccattgttg ttaggcgtcg
      721 ctgtatcccg ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgca
      781 tacggctttc tgtcgctgct ttactttcta atagttggaa gttatcggcc ccaacttctg
      841 tcacatacac acgttgtcct tgctggttgt cataagtccg tgtttgaatt cgtccgacaa
      901 cgccgatcaa gctgcctttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa
      961 tcacgcagtt aataaaatcg gcttctcgat tgccgttttg attcgtgaaa ttacggttaa
     1021 cagctagggt aaaagtcgcc actgctgtgc cacttgcggt ataacgtaaa tctggatctt
     1081 tggttaatcg tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt
     1141 catttgttgt gatctcttaa aattttgttt tcgttctgcc ttctcttgct ctacaattga
     1201 ttgcatcagg gaatcaacgg aagttagtgt agattcgaaa gaaatgtctt tggcaaaata
     1261 gttttcttgt tggtatcttt tccacttttc tctcaagccc tcatctttca ccaattgagg
     1321 catttcctga tcatacaaat gcttaaacac cgattgatta tttctttcct tgaatgttcc
     1381 ttgaacactc ttatacaaat ttttctcatc gatacgttct aatttcggaa tcatatacag
     1441 gtcataaaaa tctttcatgc gcgaattatc ttttccaaaa ctaaacgtgg catgtaattt
     1501 ttcagctaga atttgttccg tgggataagc tggaatatga atcgttttat tttcaaacat
     1561 taatttgtgt tcatacatca atgccgctgg gataatagaa tcacctgaag taatatctaa
     1621 tttaaaaggt acacgtgcat tttcaagaaa agcaataaac tttgcttgaa aacctgggta
     1681 ataatcggct tccctcgtct ctgttaatcc ctttaattca aaccgaattc catcttttgt
     1741 aggattagct gttaattcag aaaaaacagt ttctaacttt tcttttgtta gtttcgaatt
     1801 tcgataagtc gtatcaatat caacggttga acggttttct aacccgtatt ttgatccgat
     1861 cagaaatccc ccttttaata caaaatcgtc atgataaggc gattcaacga tacattctaa
     1921 taatttttcc atcatataat atctttggac aatttgtggg tttaaatgtt tctctgctgc
     1981 gaaatttctt gttttcgctt taaattgtgt cggcgtcatt cacttttact cctctcaata
     2041 aagtggcaac atatacgaag tcatttcttt tggtgatttt aaaatatcca tgtatcttct
     2101 cagcttattt gtatctctac tagtacttgt catatattct tttaatgctt cttgttttac
     2161 ttctacactt gctttatatc ttggattcca catatcacat aacgttcgct ctaaatcata
     2221 gattaaaatt ttattgccgt aaaagctttc tgtttctgtg actcccaggt caaaattttc
     2281 tttattagtg taatgcggtt gaatggcata ctgatctaat ttctcctttg atacattgta
     2341 tcctttagga aacatcatat tgatcttttt agggatatat gttcctactt gaaaaagatc
     2401 gagcgcagtc tcatgagaaa aaatccctct tgaatagata ctttgagcga gatacaattc
     2461 atccacgaac gcatctttta aggtatagaa acctggtgct accttttcta ttttatcctt
     2521 ttcggctaat cgatctaacg tcataggcga taatccttca cttctagcat ccttcaagga
     2581 taggttgcca ttatgtttat caaaaataca ttggattttg tctttcatta ctcttcccct
     2641 aaagttaata taaaactaac acaactataa catgttagtt ttatattaac aataaagaaa
     2701 tttatcgatc taattcaatt tctttttgaa tccgttgctg gttcttcaat tgctgcagtc
     2761 ttcgtttgct gacacgcatt ttttcctgtt ctgaacgaat tttggctttt tgattttcga
     2821 tttcttgttg tttgtgtgtt gtattacgca taagattaac cctccattat tttgtagtgc
     2881 gtcgtgttct cttcttgaag ttcgtttttt tcttttcaga aaattcaaag cctgtagtga
     2941 agtcttcttt taaaacaatc gttgcttcaa acgaaccctt ttgtcctttt aaccctttta
     3001 ctgtctggct tgttcggcca ttttgaatga gttccttcag ctgtgtcgct gtgagtttct
     3061 ttttggcaat cgttgtccat aatttaaaat cacaagcttc agacgtacat gcagccacct
     3121 tttgatagag taagacttct tgttcctggc attttggaca aattcctaaa tgacgcgcgg
     3181 cttcttgctt ttcactggct gctttcactt cttgtaacgt cgatccaaaa tcagtggatt
     3241 ggatttgtcc tgggacgact tccaataagt gagagacaaa tttttggata ttggctagaa
     3301 aagtggtttg tgttcctttt cgttctccaa tctttaacaa gtagctttcc catttggcgg
     3361 tcatttcggc gctcgtaagc agcggctctt tttccacggc ttggcaaagc aaaatccctt
     3421 tattcgtcac cacgagctta tttttctcta ccttgatata ttccttttgt ttcaaggttt
     3481 caataatatt cgcccgtgtc gcttctgtac cgattccttc cacttctttc aatatggatt
     3541 gcgcttcttc actatccacg gttttcccag ctgttttcat cgcagtgatc aacgtacctt
     3601 cggtataggg ttgtggtggc ttggtttctt tttcaaggac ttgtagatct accgtcaccg
     3661 tttctccttt tatcacttta ggaagtagcg gttcttcttc cttctctttt ccgtcgctct
     3721 ctttgccaaa taacttcttc caacctagat ctatcggtac ttttccgatt gcctttaact
     3781 gaagctgctg aacttgcgtc aagatcgttg tttcttcata ggtatatttt tccgcaaaca
     3841 tggccaccgt tgtttttaac acttgttcat aaatggcctg ctgcagtgga cttagttgtt
     3901 caaaagccgc tgctgtcata accgtttttg taggaatgat cgcgtaatgt tcctggactt
     3961 tcttgttatt cacataccga ctattcggcg tatgaatagg ggttgggatt gattcggcct
     4021 ttaaaaaatg cttgtattca tctaaatgat ccaacaaata cgcatactcc ccttccgtaa
     4081 tataaggggt atctgttcgc ggatagctta gatatttccc ttcatacaag ccttgcatgg
     4141 cttccaaagt gtctttggca ctagctttca tgagttgatt cattttagac tgaaggctag
     4201 atagagaaaa aagacgtgga cttgccgcac tttttttctc ggtttcgact tcaagaatcg
     4261 tgcctggctg ttttcccaat tgaaccccct tttcttttaa ataatcttca aatgcttctt
     4321 gcctcgcttg ggtttcattc ggatcaagct tcgcaacaaa tgcgccattt tgagcgatta
     4381 cttgggcttt tccttcaaaa taaggctctt tttggaagtt tctaatcttt tcctgcaact
     4441 gataaatcat gtaaagcgtt ggggtttgga ctctacctaa ggaaaaactt cctggtatgc
     4501 ctttttgctg caagagtaaa ctatacagcg ggctgccatt cataccgatt aaccaatcgg
     4561 ctatctgacg tgcttgtgct tcttcaaatt tgggataggt ctcttttgcg tctcgtaatt
     4621 gctgaaaccc ttgataaata gcttcttttt ctaaggagtt gatccaaagt cttttaaagg
     4681 tttttccttg atcggcgcct gcttgttgaa tgatcgtcca agcgatcagt tccccttcac
     4741 gatcgctatc tgtcgcaata ataatggtgt cggccgattg aagttgttgt ttcaccacgt
     4801 tgaattgctt tttcttatct ttaggaacat gatagcgata atgtgtaggg aaaataggca
     4861 aatattctaa tgaccattgc ttccaatttt catcgtacat atctggcgaa tctaactcga
     4921 ctaaatgacc aaaaccatac gtgatcacag tttctcccga aaacaatcga tcttggatct
     4981 caaaataccc gtcttttcgg gtggctttag agaagctatc ggcataagat ttcgcttgtg
     5041 acggcttttc tgctaaaatg acggttttca tcatgacggt ttcactccct ttcttgttgt
     5101 ctgccttgtt gaaacaacat ggcacgtttt tgctttctga tttcttgttg ctggatctca
     5161 tgcttcaatt gatcgttcca atcctttttc acttcaggta caaattcatg ttcatgattt
     5221 ttatagcctt tgtagtattg ttctgcttta taagagaaaa gaatctgcgc attaatatcc
     5281 cctttttctt gataaaaacg atcaaaatcg tacgtaccat ctacgcgttc acaagctttt
     5341 aattcttttg cgacttctct acaaatatct ttcaaattaa tggatcctat tgaatcgtta
     5401 acagaaagct gctggccaaa agacgcattg actttgtgag cggaaaccag ttggttcgtt
     5461 aattgcagat tgttttctgt cttgtgtatg gccataatag cttctggtgt gaccttttct
     5521 tcttttgcga cttcttcata aatgattttg tattcttttg gcacttgcca atcattagcg
     5581 attcctcgtt cacaacgtac tttctgattc gtaaatggat cgactgcgcc cattaattgt
     5641 tccttttcgt agaaaatatg tcctgcacga tcattatcca cacaaatctc aatcgattga
     5701 ggaaaggctt gtttctttct caattcagat actgcctcac caaaataatg actgatcaca
     5761 tggtgtttta acccttccat agagactaac cacgtatcgt ttaactgatc acgattcagt
     5821 gcagcgtagc taagtaaatc aatactactc tcaaaaaatt tcaactgttt aggatcacca
     5881 atccttaggt taaagccatg attcgctgtc gaatttttat ctatatgctt atacgttcct
     5941 ctctcaccaa aacgttcata atcaataaac gttccttgat aactcgcccc cacagtctct
     6001 tttgtctgtg gatctttcca taaaaaggca accgcatgat ctaaacgatt gtctcgaact
     6061 aaaaaatttt gccgttcatg ttttggcttt aattcggcaa gcaatccttc tttttctgca
     6121 taatcaatca gccactctgg cagctttcgt tcttccttca gataattcct tcctttttct
     6181 gtaagtggtt gataatcaac ggggtgatct ttgtactttt ctgtctcaaa atgaagattt
     6241 tctactcgtt ctgttttggc ttcattcttt agaataaaat cagtggctgc tttaaattgt
     6301 tgcttagggt cttggatctc tttgtctata aaaaaaagtt tagctaattc aataatgtcg
     6361 ccactaatat tttgactatt ccaataaaaa cgttgttttc ttcgatcaaa aacaaaagaa
     6421 tcatggtctt ctaagcgata gtcacgtcct ttattgacta cagccattcc attgtttcgt
     6481 gcaaaatcca caatatccat ttgcttgcat cgatcaatga gactcatttt tttctctgtc
     6541 attttcaaac ctcccaaaag aaaaaagact atccattttt cagatagtct ttcttgcttg
     6601 ttttatacgc gttctttttg tgacaattgt tgttcataat agatttcatt ttttcgtttg
     6661 gccacttctg acactgtctc aagtgaaaat cggaatggat ttcctagcgc aaattcttct
     6721 gaaggtaacg ccgtttggat ctcttttcca taactttttc cttttaaaat tacttgatta
     6781 tgccgcggat cttcaataaa cgccgcatcc ttcaaatgta acgttcgacc cgtatcttct
     6841 tccattactt tcccgtcttt cttgtcgatc caaagtgaac caacaggaac aatattcttt
     6901 tgttctgccg tttctacagt atttttatgc tcctgct
//
LOCUS       DAJSVF010000114         6921 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSVF010000114 DAJSVF010000000
VERSION     DAJSVF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.119"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            239..1024
                     /locus_tag="ON550_002558"
     CDS             239..1024
                     /locus_tag="ON550_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCR3955584.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(1097..1744)
                     /locus_tag="ON550_002559"
     CDS             complement(1097..1744)
                     /locus_tag="ON550_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3955585.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1752..2438)
                     /locus_tag="ON550_002560"
     CDS             complement(1752..2438)
                     /locus_tag="ON550_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955586.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2444..3517)
                     /locus_tag="ON550_002561"
     CDS             complement(2444..3517)
                     /locus_tag="ON550_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3955587.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3739..4413
                     /locus_tag="ON550_002562"
     CDS             3739..4413
                     /locus_tag="ON550_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3955588.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4418..5758
                     /locus_tag="ON550_002563"
     CDS             4418..5758
                     /locus_tag="ON550_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3955589.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5907..6893)
                     /locus_tag="ON550_002564"
     CDS             complement(5907..6893)
                     /locus_tag="ON550_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR3955590.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2125 a         1309 c         1208 g         2279 t
ORIGIN      
        1 attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt
       61 gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc
      121 aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt
      181 tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat
      241 gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat
      301 aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag
      361 cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa
      421 agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc
      481 cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt
      541 tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc
      601 acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt
      661 ttattccact ctactgaatc agaatctgat ttattattta tctagctata tcttttggat
      721 gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag
      781 cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt
      841 gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct
      901 tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat
      961 attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt
     1021 atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac
     1081 acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt
     1141 cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta
     1201 cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat
     1261 gattgattct taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc
     1321 tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga
     1381 tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat
     1441 gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt
     1501 tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg
     1561 acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa
     1621 atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag
     1681 tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag cccttcattg
     1741 tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga
     1801 taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt
     1861 tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc
     1921 aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca
     1981 attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac
     2041 tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta
     2101 acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt
     2161 tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca
     2221 gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa
     2281 atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct
     2341 tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat
     2401 gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg
     2461 taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa
     2521 tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc
     2581 ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc
     2641 caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg
     2701 ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga
     2761 ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg
     2821 cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg
     2881 atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt
     2941 cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg
     3001 catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag
     3061 gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat
     3121 tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta
     3181 ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga
     3241 ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc
     3301 ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt
     3361 gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg
     3421 acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta
     3481 atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga
     3541 ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact
     3601 tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat
     3661 gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca
     3721 aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc
     3781 aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt
     3841 gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg
     3901 ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca
     3961 gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt
     4021 gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca
     4081 ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt
     4141 tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa
     4201 gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag
     4261 atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat
     4321 atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta
     4381 cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa
     4441 gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt
     4501 gggattcatt actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt
     4561 agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg
     4621 cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt
     4681 aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga
     4741 gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga
     4801 tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc
     4861 cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat
     4921 ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat
     4981 gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt acttgtctag
     5041 tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc
     5101 ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac
     5161 caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac
     5221 gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg attctgatca
     5281 gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc
     5341 atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt
     5401 agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc
     5461 attaaaatat acgaatccag aagataagat cattttggat tcatctgtta accataaaga
     5521 atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat
     5581 ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct
     5641 tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa
     5701 ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg
     5761 cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata
     5821 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact
     5881 tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca
     5941 tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca
     6001 tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca
     6061 tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt
     6121 ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc
     6181 ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt
     6241 gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct
     6301 agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca
     6361 ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag
     6421 cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc
     6481 attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc
     6541 tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga
     6601 tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta
     6661 tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt
     6721 tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta
     6781 taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat
     6841 gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt
     6901 cctcctagca aatagattta t
//
LOCUS       DAJSVF010000115         6912 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSVF010000115 DAJSVF010000000
VERSION     DAJSVF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.120"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..946
                     /locus_tag="ON550_002565"
     CDS             <1..946
                     /locus_tag="ON550_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3955591.1"
                     /translation="FLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMI
                     KGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIG
                     IILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKF
                     MAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNM
                     IFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLIL
                     HILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            959..1966
                     /locus_tag="ON550_002566"
     CDS             959..1966
                     /locus_tag="ON550_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3955592.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1978..2982
                     /gene="neuB"
                     /locus_tag="ON550_002567"
     CDS             1978..2982
                     /gene="neuB"
                     /locus_tag="ON550_002567"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR3955593.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2979..4127
                     /gene="neuC"
                     /locus_tag="ON550_002568"
     CDS             2979..4127
                     /gene="neuC"
                     /locus_tag="ON550_002568"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR3955594.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            4100..4777
                     /locus_tag="ON550_002569"
     CDS             4100..4777
                     /locus_tag="ON550_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3955595.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            4767..5909
                     /locus_tag="ON550_002570"
     CDS             4767..5909
                     /locus_tag="ON550_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR3955596.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5922..6899
                     /locus_tag="ON550_002571"
     CDS             5922..6899
                     /locus_tag="ON550_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3955597.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         2529 a          872 c         1309 g         2202 t
ORIGIN      
        1 ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg atgataaata
       61 tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag taatttattt
      121 aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat acgctttaaa
      181 aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac aattcgatag
      241 aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct acattagtaa
      301 cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg ttccatggtt
      361 ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta actattattt
      421 agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt tagcaaaatt
      481 tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa ccttaggcta
      541 tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga aaaaaacatc
      601 gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa acatgatttt
      661 catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt attttttact
      721 ttttattttt cattataata tcgctaaaaa aattacgaat aaacaattat ttgacacaaa
      781 aaaaatatgt atcataataa tttttgtatc aattttttct ttgatacttc atattttaat
      841 caatcaaatg ttgattcgtt acgttttgac atcattattg ataattagtt tagttatata
      901 ttttttaaag attaagaatc gaattataga tgactataat tcataggagg agatataagt
      961 gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt
     1021 tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa
     1081 acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga
     1141 tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc
     1201 tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa
     1261 tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt
     1321 aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt
     1381 gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc
     1441 ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa
     1501 acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat
     1561 gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga
     1621 aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa
     1681 aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag
     1741 gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga
     1801 aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga
     1861 ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca
     1921 ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg
     1981 aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta
     2041 gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca
     2101 tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat
     2161 cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat
     2221 tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt
     2281 gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca
     2341 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
     2401 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
     2461 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
     2521 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
     2581 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
     2641 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
     2701 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
     2761 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
     2821 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
     2881 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
     2941 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
     3001 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
     3061 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
     3121 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
     3181 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
     3241 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     3301 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     3361 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     3421 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     3481 tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta cctagtttta
     3541 gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat
     3601 ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt
     3661 caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat gctggtagcg
     3721 aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat attacgaatt
     3781 tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc ggaaattctt
     3841 cttctgggat aattgaagct gcatcattca aaataccagt aataaattta ggaaccagac
     3901 aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg
     3961 aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga
     4021 acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga
     4081 atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga
     4141 tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt
     4201 agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa
     4261 atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa
     4321 atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat
     4381 gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc
     4441 aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg
     4501 agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta
     4561 tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca
     4621 aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg
     4681 cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc
     4741 taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata
     4801 tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat
     4861 taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg
     4921 gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg
     4981 ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta
     5041 ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga
     5101 gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg
     5161 caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg
     5221 caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata
     5281 agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga
     5341 tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt
     5401 caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag
     5461 gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat
     5521 tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg
     5581 gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt
     5641 ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac
     5701 taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat
     5761 ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta
     5821 atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta
     5881 ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg
     5941 gtttaggttc catggggaaa agaagactta gactcattca agaacacttt aatgatattt
     6001 tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa
     6061 taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg
     6121 tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat ttagattgta
     6181 atatctttac ggaaatcaat ttattaaatg aatattatga tgaaataatc gaagtagcta
     6241 aaaagaaaga tagattactt tatctatctt ctacattttt gaagagaaaa gaaattcaat
     6301 atattaagtc taaagtgacg cataataaaa aaatttctta tcaataccat gtaggacagt
     6361 atttacccga ctggcatcct tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa
     6421 ctaatgggtg tagagaaata ttcgctattg aattaccttg gattatagat acatttggga
     6481 aagttaaatc gtttactgta atgacccaga aattatccaa tcttgatatt gattattcgg
     6541 acagtttcac tcttattatt gagcatgaaa ctggtatagt tggaacttta agtgtagatg
     6601 ttgtttcaag agtagctaaa agaaatttaa ctataattgg tgaaaagatt ttaatagaat
     6661 gggaaggtaa cccggattct ctattttcat ggaatgaaga aacttctagt atgacagata
     6721 ttcaattgta cgatgaggtt atgcatgata ataattattc acatactatt attgaagatg
     6781 catatttaga ggagattaaa gaatttttca cgcttttgga aaagaaaatg gaaccccaat
     6841 atacttttga aaaagataag tatattatta acttaattaa cgacattgag gtaaattaat
     6901 gatgaaagta at
//
LOCUS       DAJSVF010000116         6793 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSVF010000116 DAJSVF010000000
VERSION     DAJSVF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.121"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            36..842
                     /locus_tag="ON550_002572"
     CDS             36..842
                     /locus_tag="ON550_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955598.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            865..1095
                     /locus_tag="ON550_002573"
     CDS             865..1095
                     /locus_tag="ON550_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955599.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTADSK"
     gene            1115..2233
                     /locus_tag="ON550_002574"
     CDS             1115..2233
                     /locus_tag="ON550_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955600.1"
                     /translation="MKKVYEKCLWISYITLGIAAFLFSLAVIVGTIFLWFTNEDLSFN
                     WFMSLLSFIMLIFLCLEIREFMPSHWQDKRKEFREEIEMKSADWVEHTWLPLKRNSKR
                     KEFWVLQLWPLGKLFSYLATLMVTLIGYLVLVYMTADYLLSEPNQIAASNIFTIVMTL
                     MCVLTLFIFSYLLVQVARQNLTMGKFFKSLFDLIILGSMLTTLTNRFEQSGRTIEGIK
                     DFLMTIDQFINGYFYLFEVGFILALLSYVFINLITRSIEKEMVIDLIPTLSGYESYVD
                     FGKYEELIHADVVHTSIHEVTVKGIRTTLKDGQETKSIDRRKEVYNFYKVEASLRYGI
                     GKLSLNGRNIPQNEPYVFWRSGALEVSENLPMEEDELQ"
     gene            2230..3627
                     /locus_tag="ON550_002575"
     CDS             2230..3627
                     /locus_tag="ON550_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HCR3955601.1"
                     /translation="MSSINLNHLFEDIHHHFQQFSIEDNEELMRLFQIEKEYDYLTFI
                     CREGRIIDPKESKIISHLRRFKNYISKNKETISEIKFDGTTNLEYLVRVTYQRNRRLI
                     EDDQGVLICAGEEYDSLKFTQLKFESIGRSIYFESRPFYIRYNDFQHLFLQDEPNGLT
                     ILERRGKKQITLSYERPLTFMDGSCESIFQKINPFIKEMMKEKDSLPFTWAEIIEAKN
                     KYELLCKKYPSKKFTKKVNKYPLRHTYALIKLKPRVTRKQFRRIEAAIEQEDIDVFPS
                     ERFDFIRKNSREFVKSLLISYVQNCILPSFATIILSDLMDMSFYLNQKLEFNFKTERG
                     LLRQHNQIVEEYNLKQAKRLKTFKLKQHGKYRLLVKHLQNHPHFNLIKTNKELFMEGS
                     TMHHCVYNYLEKIQRGGSTIWKYERQEHRYTVEIEKRKYGNYEVVQCYGKYDSLPDKQ
                     ELDEIKKVVRAIPYK"
     gene            3849..4136
                     /locus_tag="ON550_002576"
     CDS             3849..4136
                     /locus_tag="ON550_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955602.1"
                     /translation="MEIKEIKVRGINKKYVQEIDRRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILITH"
     gene            4154..4576
                     /locus_tag="ON550_002577"
     CDS             4154..4576
                     /locus_tag="ON550_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955603.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            4596..6188
                     /locus_tag="ON550_002578"
     CDS             4596..6188
                     /locus_tag="ON550_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955604.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            6200..6535
                     /locus_tag="ON550_002579"
     CDS             6200..6535
                     /locus_tag="ON550_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955605.1"
                     /translation="MKRYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISITEC"
BASE COUNT         2631 a          985 c         1319 g         1858 t
ORIGIN      
        1 tgactttagt tatttcactc gttaggagga agaaaatgag taagaaaaaa ggagcaagac
       61 aacgtttcaa aaagcgaaaa agttatctta gatggctggg aatctttatg attctcggct
      121 ttttgattct tgctagtccg atttacacac ataaaaaggt agacatacca gatgaagtaa
      181 gcacgaacgc atttttaaac acgaaggaac tgacgatggt ttcaaaagaa aaaggagcga
      241 accagcaatt agtttttgaa tttgcgatcg acgatgaaga tcaaagctta ttaaaagaac
      301 tggctaatct taattatcgc gtagaagtaa agacagctaa aggggattat caagcaataa
      361 aagccaaagt gatcaaggtg tctgatgatt attttgtagt gaagttagca catgtaccag
      421 aagaatatat cgccatgcgt ttaaccatta ttcctgaaaa aattgatcct aaagtggata
      481 tgcaagaacc acaagatctg atttattacg ttcacgaaga taaggttaaa gatcaagtga
      541 aagatgaaga ttatgaacaa catgcgatca attacaaagt gaaaggatac aagaaagaac
      601 aaaaagcagc acaaaaacaa attgaatcct tccaagcaac cattgatttg aatgaagaat
      661 tagtgaagaa gctaaaagat cagttgcctt atcaggtcgc agacgatcag gaaaatactg
      721 aaaataagat taatagttac cagcaagaaa ttgaaacatc gaaaacgcaa atgaaagatc
      781 aagaagagat cattcaaaaa cttcaagaaa aaattgatcg tatgacaaaa gaaaatattt
      841 agaagaatag gtggagtaaa agaaatgaag cgagcgaact tggtcggcaa agatactttc
      901 gctgagcaga aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt
      961 caagaagtga atttatctaa cacaacaata tctgagttga agaatggtag aaagagttta
     1021 gataccttaa aattcagatc atttgaagct ttgtatttag ctgctaaaaa atactttaca
     1081 gctgatagca aataaaaaca caggaggatt ttatgtgaaa aaagtatatg aaaaatgttt
     1141 atggatcagc tatatcacct taggtatagc tgctttttta tttagtttag ctgttatcgt
     1201 cggtacaatt tttctttggt ttaccaatga agatttatca tttaattggt tcatgagtct
     1261 actgtcgttt attatgttga tctttttatg tttagaaatc cgcgaattca tgccttctca
     1321 ttggcaagat aagaggaaag agtttaggga agaaatcgaa atgaaatcgg ccgactgggt
     1381 tgaacacacc tggcttccgc tgaaaagaaa tagcaaacga aaggaatttt gggtgttgca
     1441 attatggcca ttaggaaaac tttttagtta cttggctact ctaatggtta ctttaatcgg
     1501 ttatttagtg ctggtctata tgacggccga ttacttgtta agtgagccaa atcagatcgc
     1561 agcctcaaat atttttacga tagtcatgac cttaatgtgt gtcctgactt tgtttatttt
     1621 tagttactta cttgtccaag ttgccagaca aaacttaaca atgggcaaat ttttcaaaag
     1681 tttatttgac ttaattatct taggcagtat gttaacgact ctaacaaacc ggttcgaaca
     1741 atcaggaaga acgatcgagg ggataaagga tttcttaatg acgatcgatc aattcataaa
     1801 tggatatttt tatttgtttg aagtgggttt tattctcgct ttgcttagtt atgtattcat
     1861 caatctcatt acacgatcaa tcgaaaaaga aatggtcatt gatctgatcc caacactttc
     1921 tggctatgag agctatgtag attttggaaa gtatgaggag ttgatccacg cggatgttgt
     1981 tcacacgagt atccatgaag taaccgttaa gggcatcaga acgactttaa aagatggcca
     2041 ggagacgaaa tcaatcgatc gaagaaaaga ggtttacaac ttctataaag ttgaagcaag
     2101 cctacgatat ggtatcggaa aattgtcgtt aaatggaaga aatattccac aaaacgaacc
     2161 atacgttttc tggagaagtg gtgctttaga agtatctgaa aatcttccta tggaagaaga
     2221 tgagcttcaa tgagttcgat caatttgaac catttgtttg aagatatcca tcatcatttt
     2281 caacaatttt caattgaaga caatgaggaa ttgatgcgat tattccaaat agaaaaagaa
     2341 tacgactatt tgacatttat ttgtcgtgaa ggaagaatca ttgatcccaa agaatcaaaa
     2401 atcatttccc atttacggcg attcaaaaat tatatcagta aaaataaaga aactatttca
     2461 gaaataaaat ttgatggaac aacaaactta gaatatttag taagagtcac ctatcaaaga
     2521 aaccgccgac tgattgaaga tgatcaggga gttttaatat gcgctggtga agaatacgat
     2581 agtctcaaat ttactcagtt aaaatttgaa agtattggtc gttctattta ttttgagtca
     2641 cgaccatttt atatccgata taatgatttt caacatttgt ttttacaaga tgaaccaaat
     2701 ggattaacca ttctagaacg aagagggaag aaacaaatta cattatccta cgagagaccc
     2761 ctcacattca tggatggttc atgtgaatct atttttcaaa aaatcaatcc gtttattaaa
     2821 gaaatgatga aagaaaaaga ttcattacct ttcacttggg ccgagattat agaagcaaaa
     2881 aataaatatg aattattgtg taagaagtat ccgtcaaaaa aatttactaa gaaggtaaat
     2941 aaatatcctc tacgtcatac ttatgcgctg attaagttaa aaccaagagt aaccagaaaa
     3001 cagtttagaa gaatagaagc tgcgatcgaa caggaagaca ttgatgtttt tccttcagaa
     3061 agatttgatt ttataaggaa gaacagccgt gaattcgtaa aatctttatt aattagttat
     3121 gttcaaaact gtattttacc aagttttgca acgattattc tatctgattt aatggacatg
     3181 agtttttatt taaatcagaa actagaattt aattttaaaa cagaacgagg attattaaga
     3241 cagcataatc aaattgttga agaatataat ttgaaacaag ctaaaagatt aaagacattc
     3301 aaattgaaac aacatggtaa atacagattg cttgtcaaac atttacaaaa tcatcctcac
     3361 tttaatttga ttaaaacaaa taaagaactc tttatggaag gatcaactat gcaccattgt
     3421 gtatataatt acttagaaaa aatacaaaga ggtggttcaa ctatttggaa atatgaacgg
     3481 caggagcatc gttatacagt tgaaatagag aaaagaaagt atggcaatta tgaagtggta
     3541 cagtgctatg gaaaatacga ttcattacca gataaacaag aacttgatga gatcaaaaag
     3601 gttgttagag ctattccgta caaatgatct cacctcaagt tcttcttaat ttttaaagag
     3661 cataaggaaa cgtgggaaca ctttttaaaa aaagtgttcc aataggctgt gcctattacg
     3721 tttctctaaa acaaaagtgg gtacaaaata ttcgatttat ggatagtgta tggatagtaa
     3781 aacagctata catggatagc acatggatag actccataga ctatccatac ttttttcgga
     3841 gggtgaaaat ggaaattaaa gagattaaag taagaggtat taataaaaaa tatgttcaag
     3901 aaattgatcg tagatgcgta gaattaacag agcaaactgg tcaaaaatgg agtcgaaatg
     3961 attatctaaa actattgatt gaaaacgact ttgaacgtcc tttaatggac tacaagaaag
     4021 atcaatttga tcgtttattg gaaaaattta cagatgttca gctgcataat acaaaagttt
     4081 tggaagcata cacgaacgag gtcaagaact taattgaaat attaatcaca cattaggaag
     4141 gagataaggt tctatggtta aagcgataga aaagcatatt acattttatg aagcgcacga
     4201 tgaagtttta aaacatatta tggaaactca acctacggta aaaaattatt ctgaagccat
     4261 tcgattttta tgtatgaatt acgatccaga aattgaaaaa aataataata tgacgaacgc
     4321 aaagttgaat gcaatgggaa aggaaatttc tattttaacc gaaatggttt cagagtttgc
     4381 ggatcttcat ttgaaagata caggaatact aattggtaca aattcaactg tttatcaaga
     4441 agcaaaagag cgtgtggaag ggaaaataaa agcgaatcaa acgaaaaagt attctcctaa
     4501 gatcaagaaa ccaataaatg aatctgttca tgccagagta gacccgtttg atccaacaaa
     4561 gtttttaaaa agataaaaag tagaagggga acatgatgaa taaatcgcct gcagtcattc
     4621 tcacttgtca atttaccttg cccaataaaa aatctttttc aacatacatt gattatatga
     4681 caagagaaaa agcgctggaa gaaatggaac gacgtacgcc tgaagaagaa caggaactgc
     4741 aacgaatcaa gaatgcttta gaggattttt atattcctga aggagaaact ttttcggaga
     4801 gtaagacgga taaagaattc agcgaaacgt cgaatgaagc tcaatctttg atgggaaatg
     4861 gaatcaactt tgacaagtta gaggaacaag attttaccaa atatctttct tatatgaccc
     4921 gtcactatgc gttagaacaa aagaaagaac tgacgcaaag tgaacaaaaa gaataccaac
     4981 gcttgaacca agcaataaaa aaatatcaaa cagagccaca caaaaaagct tcaggaaacc
     5041 ttcctggagt tttttctatg gcttcagacg aagtgaaagg aagtgatctg aaggagatta
     5101 agcaaatctt ccaaagagga caacaaaaag gctcaatcat ttatcaagat gttgtctctc
     5161 atgataattc gtatcttgaa aaaataggat tgtacgatcc caaaacagat actttagatg
     5221 aagaagggtt aaaagcagct ggaagaaaaa tgatggagac attattcgag aaagagcaac
     5281 tgaacgaaac aggctactgg atggcaacga ttcaccgtaa taccaaacat atccatattc
     5341 attttgcagt tgttgaaaaa gaaaatacgc gtaaacctta tacggaggtt gaaaatggtg
     5401 tgagttatat cgtaccgaaa ggtaagagaa gacaagcaac attagacgca atgaagacca
     5461 attatgtaca tgagttagaa cgtttttctt atgaaaagga tcgaatgatt ggtatggaga
     5521 aacgaaattt attgacgcgg aaaagtgatt tgcgtaacac cttaacaaaa gtggtaaaag
     5581 agccagcgcg ttacgatcga cacgcaatga acttattaaa tgaagtatat aaaaccctac
     5641 ctgacaaacg ttcggaatgg aattacgggg atgaaaaacg aaccaaactt tcaccataca
     5701 caagagagaa actcgatgag ttaaccaaat atgtgttaac aaaagaacct gaataccaag
     5761 aatatataca gatttcaaaa gcattgaaag aagaagcaaa aagtctttat ggggaaagca
     5821 agagagaatc aaaggatgca gaaaaaaatg ctctctttga tttaaaaaag cgcacaggaa
     5881 atgccattct aacagaactg aagaaacagg atagtcaaat aaaaccttta atagaaagta
     5941 tggatcagat gcaatctatt tacaatgaac gatcctttcg aacacttaaa cagttgccaa
     6001 cgaatgaata caaatatagt tacgaagaat tcatgaagcg caggaaacaa ttccaacaaa
     6061 gagttgtcac gcagcggagt ttgcgtcaat taaatcgaac gattaatgcc catgaagaaa
     6121 aataccacgc aagtaaagca tacgaagagc aacaacgaag aattcaaatg gaacaagaaa
     6181 gacagtagga ggaagagaaa tgaaacgata tttagatcaa tggaaagtta tagaaggatc
     6241 attaagagaa gaacgaatcg aacaacttcc cgattgttta gaaaaagagc atttgtttca
     6301 gataagagaa atgttgagaa acgaacagtt tgatccaaat caatttttag tcgttgagta
     6361 cccagctact ggtgtgtact gttgtaatca tgtgaagggt gaaaaatatt ttatcattca
     6421 agaatatgag ggcaagttag cgccttatta tactacttgg gaaatgaatg aagaggggat
     6481 caataacttc ccgtgtaaaa gcattgaaga gtctatttca atcacagaat gttagttgtt
     6541 aagtgaataa aagaagtaaa agagtcctga acgtaattgt ccaggactct ttttttcaat
     6601 gaattgtttt ccgccaattc aatgagtggt gcgatttgcg actacattat atcaaaagaa
     6661 agagaaggga ggaagaagaa atggcttatt caacgattgg agttgttgtt gaaaaaagca
     6721 gagacaatct cgtttttgtg acagaaattc aaacaggcag agcttttgta gtcacagaca
     6781 aagctgcaaa agc
//
LOCUS       DAJSVF010000117         6677 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSVF010000117 DAJSVF010000000
VERSION     DAJSVF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.122"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..567
                     /locus_tag="ON550_002580"
     CDS             <1..567
                     /locus_tag="ON550_002580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3955606.1"
                     /translation="FGGVAMTINTIQAYLDIPIDFFVTLNMQGLEQIVDSIGGITLTP
                     TATFTQDSYSFYQGEIINLDGKASLAYSRNRHADDSGDYGRQARQREIIMAIIEKGTK
                     VTTLATKIPSLIKSLQGNMTTNLSVDDMWTIINKYSTALGNIEQVQLKGEGQLLDDGI
                     YYEMINEESLEEVQNILQKQLDYHLNID"
     gene            617..1141
                     /locus_tag="ON550_002581"
     CDS             617..1141
                     /locus_tag="ON550_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955607.1"
                     /translation="MQNDIRGKIKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSP
                     DINNLTLLSSLYEISIDDLLKEKKEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLV
                     LLLISLIGCLISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNI
                     FHWGGSTNVEYIEN"
     gene            complement(1138..1602)
                     /locus_tag="ON550_002582"
     CDS             complement(1138..1602)
                     /locus_tag="ON550_002582"
                     /inference="COORDINATES: protein motif:HMM:NF037267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5626 family protein"
                     /protein_id="HCR3955608.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            2516..4057
                     /gene="istA"
                     /locus_tag="ON550_002583"
     CDS             2516..4057
                     /gene="istA"
                     /locus_tag="ON550_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR3955609.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            4057..4809
                     /gene="istB"
                     /locus_tag="ON550_002584"
     CDS             4057..4809
                     /gene="istB"
                     /locus_tag="ON550_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR3955610.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWHEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(4992..5567)
                     /locus_tag="ON550_002585"
     CDS             complement(4992..5567)
                     /locus_tag="ON550_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955611.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            6355..>6677
                     /locus_tag="ON550_002586"
     CDS             6355..>6677
                     /locus_tag="ON550_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCR3955612.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVN"
BASE COUNT         2300 a         1194 c         1163 g         2020 t
ORIGIN      
        1 tttggtggag tagccatgac tataaataca atacaagcat acttagatat acctattgac
       61 ttttttgtaa cgttaaatat gcagggtcta gaacaaattg ttgattctat tggaggaata
      121 actcttactc caactgccac ttttacacaa gatagttatt cattttatca aggcgaaatt
      181 attaacttag atgggaaggc ttccttagcg tactctcgaa atcgtcatgc agatgattca
      241 ggtgactatg ggagacaagc tcgtcaaagg gaaataataa tggcaattat agagaaagga
      301 actaaagtta ctacactagc aactaagata ccttcactca ttaagagtct tcagggaaat
      361 atgactacta atctgtcagt tgacgatatg tggactatta ttaataaata ttccactgct
      421 ttaggaaata tagagcaagt tcaattaaag ggggaaggac aactacttga tgacggaata
      481 tactatgaaa tgattaacga agaatcttta gaagaagttc agaatatcct tcaaaaacaa
      541 ctagattatc acttgaatat agactaaata atctatttct taatatccaa taaataaaag
      601 tcattaatag gagaggatac agaatgacat taggggaaaa attaaaaaat cacggcatat
      661 aaaaggcatc tctcaaagtg aagtagcaaa atatttaaat atatctagac aatctatttc
      721 taaatgggaa aatgatacta gttccccaga tataaacaac ttaactttat taagtagttt
      781 atatgaaatc tctatcgatg atcttttaaa agaaaaaaaa gaagttaaaa aaaatataaa
      841 agagaatagt atagagataa tagaatctaa aaataaatta atttatttaa aaaataatac
      901 aaaccgagat gaagggttag ttttattgct catttcctta atcggctgtt tgatttctcc
      961 tcttggacta attattgcgc ctgttgttct gaaaagaaat aaagcaacaa acacttttta
     1021 taaaggagtg attgctgctt cagtcatctg ttttacagta agcctgtttg tctcctatgg
     1081 acattttagt aatatctttc attggggcgg ctccactaac gtggaatata ttgagaacta
     1141 atacgtaata ttaatggaat tatctgtcag tgtagcatct agataagagt tactagataa
     1201 aattccagct ttccattcta atgtgtagct agcttttttg ctggagacga gagacagtct
     1261 cgtgctagta aaggatccgg tcactgcgac tgcagaaggg gagtaggccc taataaattg
     1321 gttattcgag actgtcacat agtaatttgc cttccacatt ccaaatttag atccagatat
     1381 tttataattt ccgttgttga cggagaacag ggagagcccg ggaacctctt ccacacgaat
     1441 caagatagga tttccttctt cgtctacctc catgaactct tgcgtgccac cttggattaa
     1501 gttatagtag actccattat ttgccacata tgcttctaca gtgtttactt caaataaaaa
     1561 gaaagataaa cataaagtaa caaccaataa aacttgtctc aaaaaatccc tcctaaaatt
     1621 aatatactca tagtttaata tatacgggtt taattagtaa gagaacattt tgtgaacatt
     1681 cttatattga aacggctctc tctacaaatg gacgcatata taagtgtatc tgggttagta
     1741 aacattcact gttaccaact ctacttacta aacagataaa atatcaacga aataaaaaaa
     1801 ccaaatcaac taatttttta ctaataacgt agataataaa atcaaaaagc aaatttgagc
     1861 ggttgcaact ataaagtgac ctgatgtaaa ccataagatg cttaattaag ctatacttag
     1921 atgagacact atacttagga agaacttaga ttagcatagc ttcctagaac aaaaaaatac
     1981 gagaggaaga ttcttttatg actaataaat atctatctgt atttactttt ttggttggct
     2041 tgttttttgt tattaatagt gtagcaagtt ctgattttga tcttagtaag attactcttt
     2101 taactgtact accaataata ataggtagtg tgttaattct tttttctgtg acttcccttt
     2161 ttagacagcg aggtaattcc aaaaagcatt agcgaaaaag ataaaagcag ttttctcttt
     2221 acccaagaga gaactgcttt ttatcatctc cacatttctt gcttaattaa ttacagcttc
     2281 ataaatacta tcaatatggc taatagggaa accttcagta agggttaccc tagatcctcc
     2341 tctagtgtaa ccagtgatta cggcatcgaa aatgtaccat ctctgcaata atttttacca
     2401 ggctcggcaa agaaatgtac cagtaagtgc aaagcagtgt accacaaata cctcttgtat
     2461 aatgtaggtg cacaccttag ggtgtgacat ttattttaca ggaggtattt ttattatgtt
     2521 tcgttatcga aggatacttg aaatgcacag tgaaggaatt agtattcgca gtattgcagc
     2581 aagcacgggg aactcacgtc agaaaattac tgaggttatc cgaaaagctg aggagaaagg
     2641 gatagcgagg gcgttgtcgg aagaaatgac cgatcaatgg ctaggcgaat ttttgtttcc
     2701 agaaaaacaa ttagaagcca atggacggca cctgcctgac ttcgacaaga ttcacaagga
     2761 attggccaaa ccaaacgtga ccttgacgct tcttcatcag gaatacgcag ttgagtgtcg
     2821 catgagtggg aaaatccctt acgcctatcg tacattttgt gactattaca atgcgttcgc
     2881 gcgaaagcat aaagccacta tgcgcatccg tcggaagcct ggtgaaatta tggaagtgga
     2941 ttgggctgga tcgccagctt atgtcatcga tcgagatacg ggtgaaaaag taaaggctta
     3001 tgtctttgtg gcaaccttac cgtgcagcca attgtcttat gcggaagcat tcctaacgat
     3061 gaaatcggtg gcttggatta cggcacataa ccatgcattt gaatattttg gcggttctac
     3121 cgacataatt gttcccgata atctaaaaac aggtgtaaaa aagaatgaag gcatcgatgt
     3181 catccttaat gaatcatatc gagagctagg ggaacattac gggtccatca ttttacctgc
     3241 ccgagtttct actcccaagg ataagcccag tgttgaaggc agcgtaggcg tcatctccac
     3301 ttggattata gctgccttac gcaatacgca ttgttttaca atcgaagatt taaatcgtga
     3361 aatcagaatg aaattaaatg aattcaatga gcgaccgttc actaagaaac caggatccag
     3421 gatcagagcc ttcgaggaag aagaaaaatt cgcacttaat cctcttccac tggcacctta
     3481 taagatgtct gtctggaaaa aagcaaaggt tcaactaaac taccacgtct cagttgaaag
     3541 catgttttat tccgttccat acgaatacat tcaacaaaat atagatattc gaatgacaga
     3601 gaatctaatt gaactatatt ttaattccat gcgaatcgca acacataaac gtctgtacgg
     3661 gaaatttggg cagtattcga ctgtcagaga tcatatgcca gataatcaca aaatgtatgt
     3721 ggaccatacc ccagaatcct cgatggaatg ggctgaaacc atcggttcct caacagtaaa
     3781 ggttgttcaa cacctcttag accgttatca aaatgaaaaa caagccttaa agtcgatcat
     3841 gtctttgaag agctttggta aaaagtattc taaaaatgaa atagaaacgg cctgttcaac
     3901 agctttaaat ctggcttctt tgccttctgt taaaattatc cagaccattc taaagggcaa
     3961 caagcagaag cgagcaacaa gccaatcaca tattgaagac actaacattg aaaagcatgg
     4021 attcactcgc ggtgcgtcct attttggagg gaaataatga tgttaaatga gcaaacagaa
     4081 gtaaaattag ctgaattgaa actttcaggc atgcttgaat cctatcgtga acagatgaca
     4141 aataaagagt accaatccat gagtttcgaa gaccgcttca acctcatggt tgatctggag
     4201 cattcccggc gaaaaaataa taaactacag cggttgatta aatctgcaaa ctttagaaat
     4261 gcctctgcca gcattgagga tatcgaatac catccggacc gtaatttaga taagaacctc
     4321 attttaaaac tagccagtgg gacttatttg gaagaacacc ataacatcat tttgatgggc
     4381 gcttcaggga atgggaaaac gtatatcgcc aatgccttcg gaatacaggc atgccggcaa
     4441 tactacaacg tgaaatacat ccgactccct gaactactag atgaattaac catctcgaaa
     4501 aatcaagcag atggtagttt tcgaaaaata atcaacagat ataaaaaagt tcaattactc
     4561 atcatcgatg aatggctatt aaccaatctt tcgttagaaa attccacctt tctattggaa
     4621 ataatcgaat cccgtcttaa aacagcatca actattttct gttctcaatt ccatccagaa
     4681 ggatggcacg aaaagctcgg caacccccaa atcgctgatg ccattctaga taggatcata
     4741 catgattctt atcaaattct ggtagacggt aaagtttcta tgcgtgaacg ttacggcatc
     4801 cgtaattaaa tgagtcatta aaataaatta agagccactc catgattttc atgagtggct
     4861 cttagttgac cttgtggtca ttattgtcgc ttttattatt tgaaatggta accgcgtcgc
     4921 acttactggt acatttcttt gcacttagtg gtaaaaaaca atgctctagt ggtacatttt
     4981 cgatgccgta ttcaaccagc ttgaataaag gcactaacac ctgctgctgc atcatcgcaa
     5041 tggccagata aataatcccc tgtaaccatg cctaataata ctcctaacaa tcccccagct
     5101 gtgccaacta aggtacctcc tactttcaaa gcaccagcat tatacttcaa ctcgctacgc
     5161 aatgcattta atcctctgta agaagtagca tatgagttaa ccccaccatt cacaaattta
     5221 gttgtgaaag catcttgtga attaacaaca gctgtcaatt cagctccatc atatttttcg
     5281 actaaagaaa aaaattgctc ttcactaata gaattatctt gactagaagt agataatact
     5341 gattgtgtag aaaataaatc agggactgaa acagttgatt gattggaagc aatcaattca
     5401 gctaactctt cttcaaactg aatgtaaaga tcatttttta caatttcata actaacagaa
     5461 tcttcctgtt tttcagaagc tttggctatt gtaggagtaa ttaaggtcga tgtagtaatt
     5521 ggcaataagg ctaaaatagt aaataaatac ttaaatttat ttttcatttt ttatccttct
     5581 ttctattttt tctactttta ataccacaac ttgaaaaata aaaaatctaa gcaagagtaa
     5641 catctcttac gtaatgattt tgcaatagtt tttcctcctc tcacaatcta ttattatgtt
     5701 tttcgagctg tttcaaaagc acttttctat ttacatctgg gaaactaatc gttgctttta
     5761 taaaaaaaga tgatagcaga aaaaattgaa cttttcttta ctataaggta tttataaaat
     5821 catcttcata ggtcaaagta gtagtattaa tctcaacttt gataagctgg tgtatatcaa
     5881 ttttctcttt aatatgtaca atcattaaaa atagattttt ttactattta tggtttcaat
     5941 taacaaaaga acctccacac taaatcacta taaatttatt atcaccttag ttttttatgt
     6001 gctcctagca tggaaatccc atcaaaaaag taaggtttgt attcaaatgt ttttcgccaa
     6061 cgagggtatc ttctttttta cactctttgc tgtatcaaaa tataactgaa attctaagtc
     6121 tcttctatat cagtgattat gtgattcgaa atatccattc cctcaaaaga agttcatata
     6181 gaaagtgaat agagagtggc tcctgtaaat atgatatagt taaactggca aaataaaata
     6241 tgctttttca aaaagtaata tttttgtttt aattacgaag aactactttt aaatactgta
     6301 agcaccttgg cttgttagtt aactattctt aaaatgagag ggagacaaat atccatgaat
     6361 aaagctatat tttgggatct gttaggtacg ctgggagggg atagtagaac actgattaat
     6421 caaaatttca atttttttaa agacacaata ccggccttaa aaaaagcggc acaaagtcat
     6481 tatctaaata tcatcattac gaatcaaagt catatttcac atggacgtat tactatggca
     6541 gaatatactg atgccttaga gagcttaata aatgagttag agaaaaatga cattcaaata
     6601 acggatgtat acacttgccc tcatgctaga aaagacaatt gtgattgcaa gaagcctcaa
     6661 cctacatttg tcaatga
//
LOCUS       DAJSVF010000118         6485 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSVF010000118 DAJSVF010000000
VERSION     DAJSVF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.123"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            151..2094
                     /locus_tag="ON550_002587"
     CDS             151..2094
                     /locus_tag="ON550_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3955613.1"
                     /translation="MLSKRESQLIELLLEEENFQPASFFSEKLNVSNKTIYQDIQKLG
                     QFLNRFELKIEKKPRMGIYLIGTSTKKNDAKIFITSKEAMSTKVKYSSNYRRVYLFAN
                     LIFKTDKKRYEYFEKKFFVSIATLRKDYEEIIKYCRLAGIRITSNHVIDTQKTPEELI
                     QRTFVNYFEKNIGVGNEKCQYHFVFDQAMVKFVDDFVKNFYQQSKQQPTNYIVHSLKL
                     SLLTLIGRVKHGYHSQTNKNPLFVEIKKMELYMVALDFSNGVKDELNCSFTINDLYFI
                     CSLFMGHGVRPFVNLATHSQQIMETTKQFIQDMSTLVEKDLGQDSQLLQSLFAHIEPM
                     VYRLQMGITIKNPLKEDIIRQYSTMYTLATYCIPKLEKAMGIRVTDDEITFLTIHFQL
                     AFEKIVNTKHVFLICPTGLGTSQLLFQRIRHSSPSTVVYEVIDVQKLQDYNLDSVDLI
                     ISTVKLENEYQTPVIYVRPLPTKEEIALINRHLLTLNQQDNTFLPSIAPDKTILKKYL
                     KDGWMFLNQDFDSRESVLSFITEKYKEEQLVKDNFIEVVLQREKLGATGLSSGVAIPH
                     ANPKYVVKTKISIITLKNKITWGDVDIRVVLFLAIAEEDMIQVKNMIALLYDVFVSPE
                     RIKSIASKETRNEIINEIIGKRK"
     gene            2096..2557
                     /locus_tag="ON550_002588"
     CDS             2096..2557
                     /locus_tag="ON550_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3955614.1"
                     /translation="MTSYFGKKIALFQSEVKSQKEALSIIATCLYQTGLVKDSFAENV
                     LKREEVFPTGLLINDVGIAIPHTDAEHVNDSQIAFMSLKTPVTFYEMGTADKEISVSL
                     IFMLALKEPHAQLEMLQKLITMFQKKEVVQKLRAVNNTEEYYQIIEKEGFV"
     gene            2581..3927
                     /locus_tag="ON550_002589"
     CDS             2581..3927
                     /locus_tag="ON550_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3955615.1"
                     /translation="MLDLLQKFVDLGSIVVLPILIFVFGLILGTKPSKAFISALTVGV
                     GFVGLNLVIDLLTNSLGPAAQAMVERFGLNLTTIDVGWPAAAAISYGTVLGSLAIPVA
                     IVVNVLLIIVGLTKTLNVDIWNIWHAAFVASLVYAVTNDFAMGMLATVIYTMMILLFG
                     DILGPIINKFYGFPNITFPHGTSAPGFVFALPLNWLFDRIPGLKDWHADPETIQKRFG
                     IFGDSTVMGFLIGLVMGILAGYDVAGIGQLAVKTGAVMVLMPKMVALLMEGLTPISES
                     ANSFVQKRFPGRELYIGMDAALSVGHPAVLSSSLILVPITILLAVILPGNTTLPFGDL
                     ATIPFLICLMAAVFGGNIIRTILGGTIYMVTILYITSWVAPFLTKAAQGANFDLQGHS
                     SVTAMAEGGLWTTLLYVGGAKLFGYFALVGIGLIITGLLFYVNRILPKRAAKQTEA"
     gene            3950..4234
                     /locus_tag="ON550_002590"
     CDS             3950..4234
                     /locus_tag="ON550_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3955616.1"
                     /translation="MKKLLIMCGTGVATSTVVTGKVKEWLKSKGLENEVKLYQSKVAD
                     EIGKIDDYDIVVSTTVVPDNIKDKVIMGLPLLTGIGIDGLFDEIEAKIKA"
     gene            4261..5088
                     /locus_tag="ON550_002591"
     CDS             4261..5088
                     /locus_tag="ON550_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR3955617.1"
                     /translation="MLVTTKEMFEKAQKENYAIPAANFFDLDSARTFVEVAEKMQKPL
                     ILAFAQAHIEECPLEEAALIATYLAKKASVPVALHLDHGQDEEIIKQAIELGFTSVMI
                     DASQDEIETNIRRSKAIAEYAHERGVVVEAEIGHVGTGNSYEFKETTDTIYTEVEEAA
                     RFAKETGVDSLAVSIGTAHGQYKGTPKISFERLEEIKNEVSIPLVLHGGSSSGDENLH
                     KCAVSGINKINIFTDFITAAMDVINEEKPEDYFTLKRLANQAIADTLSHYYEVFATK"
     gene            5101..5796
                     /locus_tag="ON550_002592"
     CDS             5101..5796
                     /locus_tag="ON550_002592"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3955618.1"
                     /translation="MRTVRKPFFVVNPKSYLYGEQALKLAKTADQLAAAYDIDIIFTC
                     QHVDVRLIKEQTTQLIVSVQHIDGIVPGRGMGHVLPEGVVAAGAEATFLNHAERPLSV
                     SELCDAVKRAKELGLLTIVCANSLEEAKLVASLKPDVMVCEPTELIGTGQASDISYMI
                     QTNEAVRKMDPEIFVLQAAGISTVADVRKALDSGADATGGTSGIVCAKDPVDTLKAMI
                     EEVSKWKKEQSTK"
     gene            5793..6308
                     /locus_tag="ON550_002593"
     CDS             5793..6308
                     /locus_tag="ON550_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016380999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR3955619.1"
                     /translation="MITYTEQLVLTSNGQRVTYHNITEQVKEIVAKSGIKNGICLVQS
                     PHTTCSVIFEEFMHDLDFNGDEFLQVDLNRILDRIIPRELTENTDYRYPGPKHLDFLL
                     SLDDPDYAGDAGTILNGDAHIRASFFGASENFIVKEEILMTGTVGSIYFIDFDQNRVR
                     NRKCHILVMGE"
BASE COUNT         2152 a          986 c         1374 g         1973 t
ORIGIN      
        1 taaaaaaatt gtcgtgagtt ttactgacat caaaaagtat agggtcataa taatctgttc
       61 cacattaatg tggaacagat tacgtattga tctttagtga gaaaaactct atgatataga
      121 caaggaaaac gttttctgag gtgatgatga atgttatcaa agcgcgagag tcagttaatt
      181 gaattacttt tagaggagga gaattttcaa ccagcaagtt tctttagtga aaaactcaat
      241 gtttcgaata aaacaattta tcaagatatt cagaagctgg gacagttcct gaatcgtttt
      301 gagttaaaaa ttgaaaaaaa acctcgcatg gggatttact taattggaac gtcaactaaa
      361 aaaaatgatg ccaaaatatt tatcactagt aaagaagcta tgtcaacaaa ggtaaagtat
      421 agttcaaatt atcgtagagt gtacttattc gccaatctaa tttttaaaac ggataaaaaa
      481 agatatgagt attttgaaaa aaagtttttt gtcagtattg caactttgcg taaggactat
      541 gaagaaataa tcaaatattg tcggttagct gggattagga ttacttcaaa tcatgtaata
      601 gacacacaaa aaacacctga agagttgatc cagcggacat ttgttaatta ttttgaaaaa
      661 aacatcggtg tagggaatga aaagtgtcaa tatcactttg tttttgatca agcgatggtt
      721 aagtttgtgg atgattttgt aaaaaatttt taccaacagt ctaagcagca accaacaaat
      781 tatattgtcc attcactcaa actttcgttg ttgacgttga ttggtcgagt aaagcatggc
      841 taccattcac aaacaaataa aaatcctttg tttgtcgaaa taaagaaaat ggaattatat
      901 atggtggcac tagatttttc aaatggggtc aaagatgaat tgaattgttc ctttacgata
      961 aatgatcttt actttatttg ttcgttgttt atgggacatg gagtgagacc gtttgtgaat
     1021 ctggcaaccc atagtcaaca aatcatggag accaccaaac aattcattca agatatgtcg
     1081 acactggttg agaaggattt ggggcaagat agtcagctgc ttcaatctct atttgcccat
     1141 attgagccaa tggtttatcg gttgcaaatg gggattacca ttaaaaatcc tttaaaagag
     1201 gatatcattc ggcaatacag cacaatgtat acgctagcaa cttactgtat tccaaaattg
     1261 gaaaaagcaa tggggatcag agtaacggat gatgaaatta cttttttgac tattcatttt
     1321 cagttagctt ttgaaaaaat tgtgaatacg aagcatgttt ttcttatttg tccaacgggg
     1381 ttgggaactt cgcaattatt atttcagcgt attcgtcata gttctcctag taccgtagtc
     1441 tatgaagtga ttgatgttca aaagttgcag gattacaact tagatagtgt cgatttgatt
     1501 atttcaacag tcaaattaga aaatgagtat caaacgccgg ttatttatgt tcgaccatta
     1561 ccaacgaaag aagaaattgc attgattaat cgtcatcttc ttaccttgaa tcaacaagac
     1621 aatacatttt taccatccat tgcaccggat aaaacgattt taaagaaata tttgaaggat
     1681 ggttggatgt ttttgaatca agattttgat agtagagaat ctgtactttc tttcatcact
     1741 gagaaatata aagaagaaca attagtaaag gataatttta ttgaggttgt acttcaaaga
     1801 gaaaaactag gagctaccgg actttcatca ggcgtagcca tcccacatgc aaatcctaaa
     1861 tatgtagtca aaacaaaaat ttctattatc actctaaaaa acaaaattac ttggggagat
     1921 gtggatataa gggttgtttt attcttagcg attgcagaag aagatatgat tcaagtgaaa
     1981 aatatgattg cactacttta tgatgtcttt gtctcacctg agagaataaa aagtattgca
     2041 agtaaagaaa caaggaatga gattattaac gaaattatag gaaagaggaa gtaagatgac
     2101 gagttatttt ggaaagaaaa ttgcattatt tcaatctgag gtaaagagtc agaaggaagc
     2161 tttatcaatt attgcaactt gtctatatca aacaggttta gtgaaggatt cgtttgcaga
     2221 gaatgtactg aaaagagaag aagtgtttcc tactggctta ttaatcaatg atgttgggat
     2281 tgctatacca catacggatg cagaacacgt gaatgattct caaattgcat ttatgtcatt
     2341 gaaaacacca gtaacttttt atgaaatggg aactgcagat aaggaaattt cagtcagctt
     2401 gatcttcatg ttggcgttga aagaaccaca tgcacagtta gaaatgttac agaagttaat
     2461 tacgatgttt caaaaaaaag aggtggttca aaagcttcga gctgtaaata atactgagga
     2521 gtattatcaa attattgaaa aagaaggatt cgtttaataa ccattttgag ggggagcatt
     2581 atgttagatt tattacaaaa atttgttgat ctcggatcaa tcgttgtatt gccaatctta
     2641 atttttgttt ttggtttgat tttaggaaca aaaccgagta aggcttttat ttcagcatta
     2701 acggttggtg ttggatttgt cgggttgaat ttagttatcg atttattgac caatagctta
     2761 ggaccagcag cacaagcgat ggttgaacgt tttggactaa acttaacaac cattgacgtt
     2821 ggttggccgg cagcagctgc gatttcttat ggaaccgttt taggtagttt ggcgattcca
     2881 gtagctatcg tagtcaatgt gttattaatt attgttggct taacaaaaac actgaatgtt
     2941 gatatttgga atatctggca tgcagcattt gttgcttcac ttgtctacgc agtaacgaat
     3001 gactttgcaa tgggcatgtt ggcaacggtt atttatacaa tgatgatttt attgtttggc
     3061 gatattttag gaccaatcat caataaattc tatggatttc caaatattac cttcccacac
     3121 ggaacatcag cacctgggtt tgtttttgcc ttacctttaa attggttatt cgataggatt
     3181 ccaggattga aagattggca tgcagatcca gagacgattc aaaaacggtt tggcatcttt
     3241 ggtgattcaa cagtcatggg ctttttaatt ggtttggtta tgggaatttt agctggctat
     3301 gatgttgcgg gaatcggtca attagcggtg aaaactggtg ctgtaatggt cttaatgcct
     3361 aaaatggtgg cacttttaat ggaaggctta acacctattt cagaatcagc aaattcattt
     3421 gttcaaaaac gtttcccagg aagagaatta tatatcggaa tggatgccgc attgtcagta
     3481 ggacacccag ctgtattgtc ttcttcgtta atcttagtcc caatcacgat tttattagca
     3541 gtgattttac caggtaacac aaccttgcct ttcggtgatt tggcaactat tccattctta
     3601 atttgtttga tggcagctgt ctttggtgga aacattattc gaacaatctt aggtgggacg
     3661 atctacatgg tcaccatttt atacatcact tcatgggtgg caccattttt aacaaaagca
     3721 gcacaagggg caaactttga tttacaagga cattcatcag taactgcgat ggcagaaggt
     3781 ggactgtgga caacacttct atacgtgggt ggtgcaaaac tatttggcta ctttgcccta
     3841 gtcggaattg gtctgattat tacaggctta ctattctacg tgaatcgcat tttacctaaa
     3901 agagcagcta aacaaactga agcttaatac aaaataggag agatgacaga tgaaaaaatt
     3961 attaattatg tgcggaacag gtgtggctac ttcaacagtg gtaacaggaa aagtaaaaga
     4021 atggttgaaa tcaaaaggac tcgaaaatga agtgaaacta tatcaatcaa aagttgctga
     4081 tgagattggc aaaattgatg actacgatat tgtggtcagt acgaccgtgg ttcctgacaa
     4141 cattaaagat aaagtaatta tggggcttcc tttattaacc ggaattggca ttgatgggtt
     4201 gtttgacgaa attgaagcga agattaaagc ctaaaaaata cttgaaaaga aggtaatcaa
     4261 atgttagtta caacgaaaga gatgtttgaa aaagcgcaaa aagaaaacta tgcgattccg
     4321 gcagcaaact tttttgattt agattctgca agaacctttg tagaggttgc agaaaaaatg
     4381 caaaagccat taattttagc ctttgcacaa gcgcatattg aagaatgtcc tcttgaagag
     4441 gcagcgttaa ttgcaacata tttagccaaa aaagcaagtg ttccagtagc acttcatttg
     4501 gatcatggac aagatgagga aatcatcaag caagcaatcg agcttgggtt cacttctgtg
     4561 atgatagatg cttcgcagga tgagattgaa acaaatattc gtcgttcaaa ggcgattgcg
     4621 gaatatgctc atgaaagagg cgttgtggta gaggctgaga ttggacacgt gggaacggga
     4681 aatagttatg aatttaagga aaccacggac accatttata ccgaagtgga ggaagcagca
     4741 cgttttgcga aagaaaccgg cgtcgattca ttggcagttt cgataggtac ggctcatggg
     4801 caatacaaag gaacaccgaa aatcagcttt gagcggttgg aagaaattaa aaatgaagtt
     4861 tcaattccat tagtgttgca tgggggctca tcatctggtg atgaaaatct ccacaaatgt
     4921 gctgtttcag ggatcaataa aattaatatc tttactgatt ttatcacagc agcaatggat
     4981 gtaattaacg aagaaaaacc agaggattat tttacactta aacgattagc gaaccaagcg
     5041 attgcggata cgttatcgca ttattacgaa gtatttgcaa caaaatagag gagggttctc
     5101 atgagaacag taagaaaacc attttttgtg gttaatccta aatcttattt gtatggtgaa
     5161 caggcattga aactagcaaa aacagcggat caattagctg cagcatacga catcgatatc
     5221 atttttactt gtcaacatgt tgatgttcgg ctaattaaag aacagacaac gcagttgatt
     5281 gtgagtgttc aacatatcga tgggatagtc cctggtagag ggatgggaca cgttttacct
     5341 gaaggcgttg tagcggctgg agcagaagcg accttcctta atcacgcaga acgtccttta
     5401 tcggttagtg agctgtgcga tgccgtcaag cgagcaaagg aacttggtct attgactatt
     5461 gtttgtgcaa attctttaga agaggctaag ctcgtagctt ccttgaaacc tgatgtgatg
     5521 gtttgtgagc ctacggaatt aataggtaca ggtcaagcaa gtgacatttc ttatatgatt
     5581 caaaccaatg aagctgtacg caagatggat cctgaaattt ttgttctgca ggcagcaggg
     5641 atttcaacgg tagcagatgt tcgcaaagca cttgattcgg gggctgatgc aacgggtgga
     5701 accagcggga ttgtttgtgc caaagatcct gtggacacat tgaaagcgat gattgaagaa
     5761 gtaagtaaat ggaaaaagga gcaatcgaca aaatgataac ttatactgag caacttgttt
     5821 tgacttcaaa tggacagcga gtgacgtatc ataatattac cgaacaagta aaagaaattg
     5881 tggcaaaaag tgggataaaa aatggcattt gtcttgttca gtcgccacat actacttgtt
     5941 cagttatttt tgaggaattc atgcacgatt tagattttaa tggggatgaa tttttacagg
     6001 ttgatttaaa tcggattctt gatcgaatca ttcctcgaga attaacggaa aataccgatt
     6061 atcgttatcc tggtccgaag catcttgatt tcttgctgtc cttagatgat ccagattatg
     6121 ctggtgatgc tggaacgatt ttaaatggag atgcacatat ccgagcatca ttcttcggag
     6181 caagtgaaaa tttcattgtc aaagaggaaa tactaatgac aggaactgtc ggctccattt
     6241 atttcattga ttttgatcaa aaccgtgtta gaaatcgaaa atgccatatt ttagtaatgg
     6301 gtgaatagaa aagtaataag aaacaaacat ttgggaaact aattgaatga tcacacctat
     6361 aaaaattaat acgaaagata atagagtcaa gtccagactc ctgtgtaaaa tgctatacaa
     6421 tgtttttacc atttctactt atcaaaattg atgtattttc ttgaagaata aatccattca
     6481 tcatg
//
LOCUS       DAJSVF010000119         6378 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSVF010000119 DAJSVF010000000
VERSION     DAJSVF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.124"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(99..1229)
                     /locus_tag="ON550_002594"
     CDS             complement(99..1229)
                     /locus_tag="ON550_002594"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR3955620.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ON550_002595"
     CDS             complement(1878..3080)
                     /locus_tag="ON550_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3955621.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ON550_002596"
     CDS             complement(3140..3343)
                     /locus_tag="ON550_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR3955622.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="ON550_002597"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="ON550_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="ON550_002598"
     CDS             4880..5692
                     /locus_tag="ON550_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955623.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1910 a         1305 c         1006 g         2157 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagact
//
LOCUS       DAJSVF010000120         6320 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSVF010000120 DAJSVF010000000
VERSION     DAJSVF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.125"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1039..1704)
                     /locus_tag="ON550_002599"
     CDS             complement(1039..1704)
                     /locus_tag="ON550_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955624.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1884..3866
                     /locus_tag="ON550_002600"
     CDS             1884..3866
                     /locus_tag="ON550_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR3955625.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3870..4508
                     /locus_tag="ON550_002601"
     CDS             3870..4508
                     /locus_tag="ON550_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3955626.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4536..4964
                     /locus_tag="ON550_002602"
     CDS             4536..4964
                     /locus_tag="ON550_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955627.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5421..>6320
                     /locus_tag="ON550_002603"
     CDS             5421..>6320
                     /locus_tag="ON550_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3955628.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTA"
BASE COUNT         2175 a          965 c         1172 g         2008 t
ORIGIN      
        1 atcaaggttc ctagcctttt tataataaaa tgaagcgaaa ataatttctt ttaaagagcc
       61 gagattatca gctatataag agtgaatttg atgcaaaaaa cagagccgaa acagtcttgg
      121 tgttcgtagc tctgcttata tgtttcgaat ttaagacaaa gagttggcaa aagatgttga
      181 taaggaagat aagataactg tgttatataa actaggtaac taactgtaac aagctaattt
      241 taagagattc ttattagaag acgtttagga atataactaa gaaaatgatt atacaggtaa
      301 ttgtcatttc aataatgttt tctaattttc gcgtctttgt tttaagaaaa aggccagaat
      361 cagttggaaa aaagttcgta cgatagtgaa gaggggcaaa atttcctact aatattccca
      421 ctaacaagag ataaaacggc caggttggtg tgattcctct ataaaaaaat gcccatgcta
      481 ccgaaatccc atagattaac aactgaacaa tttttacaat gaaaaaattg ttctgccatt
      541 actcaaagtc tcttcctcct tgtatcgttt atttgtttca ttttatcatt atgataggcg
      601 cttgaacaat ccattttgta ttttattgag tactcttgga taaactacca gcgattatca
      661 tatttattta tgttactgta agccatcata taaaagtaaa tgagcagatc atagaaatat
      721 aaaaaagaac taaaattgat ctaaattatg tgcagcttga ctacgtacca atctatatta
      781 cttactagat tggtcgttaa tctattcgga tcgaatgagc aaaatatttt tgtgaatgtc
      841 catccttgat tatgattcca aaaagaatca ttgggaggct gtggcgtaaa tattatacta
      901 taagccagaa atacctatca ttctgaactt ttttatttag gaactttcat ctcaaggctt
      961 tttcaattaa tttgtacccc tgttttttgg ataagatcat gtgcattgat tttttctatc
     1021 tctacgttca tctcctactt aatgcctatc tggctcctga gggcttcttt ttaaggcgtc
     1081 gtcatagtct tggctgagga tttcttggta atctgaggga atggcttccc aagtgagcag
     1141 gcgaacgttc atgacgtgtt ttcctttgtg tttgattaca gcatggtttt tctctttttc
     1201 tacctctcca gcgtgaaaag acagccatgt accatggcca aagatatcat tacataccca
     1261 acgttcgatg gaattgtcca cgtatcctac agggagtgtg gtatagccga tttgtggttt
     1321 caaaaaagga ttgaatcgat cgatcgtttc atttttgtat tgataattga taagaaaaag
     1381 atagaccaaa caaaagacga gcagaaagaa agatagtgtc tttttgagtg agaagggttg
     1441 ttttgatgaa taagatttct tgttaacaag aggttcatct ggcaatgttg gttctattga
     1501 ttcggtagct gattcaatag aaggaaaatc aatcggcaga cctaaagtat gatgtaaaag
     1561 tgacagcatt tcaaggtctg gcattgtacg agaattttcc caactagaaa tcgttttatc
     1621 ggaaacacca attttttcgc ctaattcttt ttgcgtaagc ttacaatttc tgcggtaagt
     1681 tcgtattttt tctccggtgt tcatcttctt ctcctttcga ttctacagta tatagattta
     1741 ttctacatga cctatacact tgatagatac gtttttttga tgaaaaacgg tatgatttta
     1801 tcaaagaatc atttaagtaa gggagagtgg tgataatcaa aaaaaagttc acagttttac
     1861 tcacaatcat agtgaccggt atcatgagtg gttttgcctt ggttagtata aataaacagg
     1921 tactctatcg gacaaatgaa tttttccatc aaacagatac ttatactccg attcgattgc
     1981 cacaacattt tattgggcaa gaagaatatg agcagctaat catggtattt gaaaatgtct
     2041 catcagaaac tgagctttct tatatgaaaa aaacagtaaa tgaatccaat gaagtagata
     2101 catcttggaa cttcaagata cagccaaagc ttgaaatgac attgtattat caaggggatg
     2161 tcaaatcttc tcacgagaag gtaccctatt ctaatacctc ctttcgcgta acgtccattg
     2221 aagaatcgat caaaataaaa gattttgaag gtgaattttt cttgatgacg agagaaaaag
     2281 aagcctatga acggttcatt tcactattca ctgatcgatt caatcaagca tttcacactg
     2341 attatgccac taatattttt gaagactttg aaaatagcta ttcgtattcg gttatttcaa
     2401 atacagacgt atataattat cgagtgtatt taactattgg attgattctt ttattccttt
     2461 tgttatcatc ctttttctta ttgatgaata aagaaatcag tttattgttg aataatgact
     2521 tttcattaca tcaagtattg tggtgtttat tggggaaaaa gaaaatcatt attacattca
     2581 taattgcagg aatcattggt ggttttattt ttatgtatta taaggagcaa tttctttatt
     2641 tgttccagat gtacgttttc ttgttcagcg gaatatatgg tatctctttg atttctcttt
     2701 tattagttga aaaaatcgct tcgttaagag aagcgttcaa ctatatccaa tacattcttt
     2761 ttacatttgg attgttgttt tctcttcttt tgaccgttgc caatgtcgaa ttagcaacta
     2821 tcgttttttc ttcttcacag ttagctgttt cccatgaacc accaaaagaa atcgctggtg
     2881 aggactatca tgtattttat ccagtaacca tcgggaaaaa ccacgtggat tttatttatt
     2941 ccaatcgttt tgcatcagca gcagatcagg aattatatga aactttgaat aaaaacggaa
     3001 gtatattagt caatgtatca gattacttga atgaagaaaa tgaacagttt ggcagaggga
     3061 taaaaataaa tctgaattat cttaagaaat ttcctttgat tgatgtctct ggaaggttga
     3121 tccaaataac cgaaaaagaa acgcaccctg ttatattgat ccctgaacga tatagacata
     3181 cagatttaaa aaatgacctg gcgcaaataa cagaatatta tcaagaaatc agtgaaaaag
     3241 aaccgaaatt tctatttata aaaaacaatc aacccattta tacatttatt ccttctatcc
     3301 cctggatcga acattatccg gttgttgaaa tactgacatt gaaaaacagt atgtatttgg
     3361 aaagaaatat tctcagcgga gaaatctatc caccgttaaa gatcaagata ggctctttgt
     3421 cgaaagaaag gttatttgag ctgattgaag aaagtcagtt acgtgataac ttgcagttat
     3481 catttccgta tactcagact gaagagatag ctgtcaaagt gttaagcaga tcatttcatt
     3541 acttgataca gatattttta ctcactttct tttctttctt cttgttgagc tatgtcatgt
     3601 tatgcatata ttttgtctac aattgtcgga aaatcgctat ctttcgttca agtggttatt
     3661 cgctctttga aacgtataaa gatttcttca tgatgaacct catcaaatgg ggaacgacaa
     3721 gtgtaatctt cctttttctt atagaaagag agcctaaata tctttttaat atttttttct
     3781 ttagcttcat tgattttatt ctttcagttg tttttatttt gtctacagaa aagaaaagtc
     3841 agttattgct gatgaatgga ggttaacaat tggagccgtt gatagtgata aaaggtgcga
     3901 aaaaaatcat tcgaaacaaa acgattcttc atttggatcg tttcactgcg gatgctgggg
     3961 atcttgtgac tgttgttgga gaaagtggag caggtaaaag tacgttattg aatatattag
     4021 gattgaatga tcagttcagt tcaggacaat tctatttgtt cgatcaagag acgaaacaat
     4081 taactgggag agagaagcgc ttgataaaaa gaaataccat atcttttctt tttcaagatt
     4141 ttgggttgat tgaatatgaa acaatcgatt ttaatttaga tattggattg aagttttcga
     4201 atttaaatgt ttcagataag cgaacagcaa agtatgaggc attgaaaaaa gtaaatataa
     4261 acaagaagct aagtacgcct atctacacat tatctggtgg tgagaagcaa agaatcgcct
     4321 gtgcgagagt cttattgaag aatagcaaaa tcattttagc cgatgaaccg actgggtcat
     4381 tggataaaag aaataaggat aaagtagttg aaatcttatc gcaacaagct tcccaaggta
     4441 cgttagttat cgtagtcacg catgatccat atctgaccaa aataggaaac aagaagatca
     4501 tgctttaaaa atcaaaaagg aaaggatgtc gttttatgaa aaaagcagtt attatttgtg
     4561 cactagggat aggttttgtt ggaggaaacg ctatgtcatc tgaagtggaa gcaaattcag
     4621 ttcacggtga aatctattac aaatctttaa agccaggaga gattcatttt gatgctaaag
     4681 gtaatattat taagtcaaat ttaccagtag accgggaaat ttctagaaca gcaattaatc
     4741 atagctctgg aagatgggtt tattatagtg atacaacttg tacgagaaaa acaggtcatt
     4801 ctaaccacta tagtcgtgtc tataatcgtc acggtgcaaa agcgaaagtt ggaaactcta
     4861 ttcgatcagc aaatgcaaca aaaggagtat gggcctatgc aagtgcgaca ggaaatagaa
     4921 ctgacacatt ctcatgttgg tacaatccaa ccggttggta ttaagtatcc aaaagtttat
     4981 ataaaataga aaaaacatat aatgaaacag cgttaaaagt tatttttggt agttttaata
     5041 tattcatttt tagttgtata aaaatatatc ataataaaaa caacaaaaaa atgtctggaa
     5101 aatcgaacat aaaagtgtct gatttgaata tcccagcagc ggaagaagaa aaacaaaatc
     5161 gataacattt gttctaagaa ttagttacgt taaatacgga acgcaagata gacttgaaaa
     5221 acggtaacaa aagaagtagt atattaaggt gtgatgtgct acttgacatt tattctgctg
     5281 agcgaacttt ctggattgtg tagccttata tctattcagg acgtatcgag cagattagta
     5341 aaggtgttct tgatgatttt catcttgaac acctttgttt ttagcaattc gcaaaaaacg
     5401 aaaagtagga gaattgataa atgaaaaaaa gatttttagc acttgccatc gtactaggca
     5461 ctggcttatt gtcaggctgt acgaatgccg gtgaaaaaac agccgttagt tataaaggcg
     5521 gtacaatcag cgaacaagaa gtaatggata gcttgaaaaa aatgcaagga gcagattccg
     5581 ctgtgcaaca gttgatcgtt tatcaagtat ttgaagataa atatggagat gacgtatcaa
     5641 ccaaagaaat cgattctcaa tatgatcaaa caaagaaaca attgggtgat tcatttgatt
     5701 cacaattgaa atctgcaggt tacacagaac aaacattcaa agacagtatc aaacaaagtc
     5761 tagcctttca agaaggtcta aagaaacata tcaaattgac cgatgaagat ttaaaaacgg
     5821 cttgggaatc tttccatcca gaagtggaag ctcaaattat ccaagtagca agtgaagatg
     5881 atgcaaaaga tgtgaagaaa gctgctgata agggagacga tttctctaaa ttagccaaag
     5941 acaagtcaac tgatactacg acaaaagaag atggcggaaa agtgaaattt gattccacta
     6001 ctacaacggt tccggcagaa gtcaaagaag cagcctttaa gttgaaagat ggtcaagttt
     6061 ctgatgtgat cacttctacc aatgcttcaa cttatactac ggaatattat gtggttaaaa
     6121 tggtgaaaaa ccaaaacaaa ggcaacgaca tggataaata caaaaaagaa ttgaaagaaa
     6181 ttgcaacaga cacaaaatta agtgacagta ccttccagaa taaagtaatt ggcgaagtat
     6241 tgaaagacgc taatgtaaaa atcaaagaca aagactttga aaatgttttg agtacattca
     6301 cttcagattc tagtacagca
//
LOCUS       DAJSVF010000121         6291 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSVF010000121 DAJSVF010000000
VERSION     DAJSVF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.126"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..348
                     /locus_tag="ON550_002604"
     CDS             <1..348
                     /locus_tag="ON550_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCR3955629.1"
                     /translation="GQPREPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLH
                     KLFVRGYSAIQHPHKPDVTERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRII
                     IQFASSLELVAFK"
     gene            623..859
                     /locus_tag="ON550_002605"
     CDS             623..859
                     /locus_tag="ON550_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955630.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            883..1224
                     /locus_tag="ON550_002606"
     CDS             883..1224
                     /locus_tag="ON550_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955631.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1217..1408)
                     /locus_tag="ON550_002607"
     CDS             complement(1217..1408)
                     /locus_tag="ON550_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR3955632.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            1618..2265
                     /locus_tag="ON550_002608"
     CDS             1618..2265
                     /locus_tag="ON550_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955633.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            2525..2869
                     /locus_tag="ON550_002609"
     CDS             2525..2869
                     /locus_tag="ON550_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3955634.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2874..3155
                     /locus_tag="ON550_002610"
     CDS             2874..3155
                     /locus_tag="ON550_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955635.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3258..3572
                     /locus_tag="ON550_002611"
     CDS             3258..3572
                     /locus_tag="ON550_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR3955636.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3550..5244
                     /locus_tag="ON550_002612"
     CDS             3550..5244
                     /locus_tag="ON550_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3955637.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5264..>6291
                     /locus_tag="ON550_002613"
     CDS             5264..>6291
                     /locus_tag="ON550_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3955638.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGV"
BASE COUNT         2282 a          867 c         1250 g         1892 t
ORIGIN      
        1 gggcaacctc gagaaccaaa agtgacagca tcttattcct tggttccacc ttctacagct
       61 gatcatatat ttgaagcaga aagaatgctg atcgataaag aagaagcaca ggaagaattt
      121 gagtatctgc ataaattgtt tgttcgaggc tattcagcga tacagcatcc gcataagcct
      181 gatgtgactg aaaggcgcaa gaagatattc tatgatcgtt atatcaatgg tctgcccatt
      241 tatgtaactg ctcaaaggaa taatactagc gaagaatcgg ttaaagtaga atcaaacaga
      301 attatcatcc aatttgcttc atcgttagaa ctggttgctt tcaagtagcc agtttttaca
      361 ctttttatac ccttttatta ccaatttggt ttccatttta tacctttttt ataccaatca
      421 cttacctatt caacgttgta ttatgatagt gtcgaaagat taggaaacag gatcgacaaa
      481 ataaaatgta agggaggaaa tctccctcat cgtttaatta agcttcgata ggcagcagcg
      541 gaaatattaa gaataaggat gtgaatttca actccttcta aattgttctt attatctatc
      601 atccgttgct gtctattaat ttatgtattg gagggtatat aaaatgaaga tcgcttccga
      661 aaaaattgat caactaacaa ttttagtaaa ccaaagaatc gaagatatta tcaaagaatt
      721 cgaaggtgaa aagattaaga acataaagct tactttaatg gatcataagt tcgaattaaa
      781 ttcgacaaaa aggaagatcg ttgagcttat agaagaagaa atagctctcc agatcaatga
      841 gcaacaatta gctaattaat ttatgtattg gaggggaaat gaatggaagt cgaacaaata
      901 aagtatcaag gtatggatcg ggaacggttg attgatatta ttgagcaaca acgtttagaa
      961 ttgatccaaa aagaagctgt atgtaaagag tacaaaaaac atttggaaca agtaattgag
     1021 tatcattcag tagaaaagta cagatcagtg gtgcaaaaaa atagagcaac gagtcctgat
     1081 tctcgtcaag atgtatcgat tagtatcaag acgccagtat ttaattctgt taaatcagaa
     1141 aagctagaaa gtatctcaac tgctttggta gaaaaagcag ctgaaatgaa ctccctagca
     1201 aatgaagtta gtcgtcttat ttagtcatat cgataacttg agagtctttg gtgattccgt
     1261 tttttataga attaatggtt tgttcagcgg aatatttagt caagtatgtt tcgctagttg
     1321 ctactacttc attgttattg gacttaataa cgaagtaata ttggccatta gttgcttctc
     1381 ttataacaaa gtacaaattt ttcaccacct ataatttatt tcagcggacc actcgctgat
     1441 aattaaaatt ataagctatg tataaaattt cacaatatca atttgtcgct gtggcggaaa
     1501 gggtagacgc aataaaggat gaatgggaaa agtcaatgag taattatctg aaatttacaa
     1561 gtcccctgtt tagttatcta agattatttg ataatattat ttctgaggtg atagaaaatg
     1621 gtgggcgtag ttaaatttct tgggtttaca tttgatggat atggtacgtt aggcgaatgg
     1681 gtaggtatta ttttgatagt gattacaatt caacaaacat caaagtacta ctggtattct
     1741 aatagttcta aaattgtatc aagaataact gaaggaataa ccaaaaaaga tattactgtc
     1801 gtcggtaaat atgggtttac tataacttta ttaaatgaag ggaaatctga aaccgcaata
     1861 aagttttgcg ggattatagg taaatataac attttccaac gttttatcag aaagcattgg
     1921 tacaaatata tttacaaata ccctgtagtt aaaaagctta ttgaaaaata tgtggaccac
     1981 gaaatatctt atctcaatcc aacagacctt attaatcaag aattccagaa tattggatac
     2041 ttagaaaaag gtctaaaaat accaattgta aatgaaagtt tagttactgc aatggtagct
     2101 aacataaaga acagcaagaa attagaaaag agatataaaa aatgtaaaaa attgaaattt
     2161 tcctttttat tcttagaata taatggcaaa gaatattatc aagatattta tgttttgcga
     2221 ggctcgaact tatataaaga cattaaaaat aagatttcag tatgagattt tttaaaattc
     2281 gtagatcact ctttgagtgg tctttttatt ttgcgtaaag gaggcacgac gatgtataga
     2341 ccacaatact tagaacagaa gcatgaagta atcactgtgc aaaacggtaa cggtgagata
     2401 gtacgaaagt atagaagacc aataaagagc gatacatata aacggaagga aagcaatgaa
     2461 gttattccat tgtatggcaa aagaatagct aagtattaaa taagattgcg aaaggagatg
     2521 ggacatgacc gaggaattct atagatggtt attacagttg ataagagaag atcgtttggt
     2581 taagttctat cagtctccta aatggcgcag gcttagagag aaagcgatga aacgagatca
     2641 ctatgaatgc caagagtgta gaagactagg taagtatcat agagtagaga acgttcatca
     2701 tataaaggaa gtcaaggata gacctgactt agctttagat ttagataatc ttatttgttt
     2761 atgtgttgaa catcataatg aagttcatgg cagatatctt acagcgttag ataaacaaga
     2821 gaagaagata gaaagcttcg ctaacttcga tgcaagtgaa aggtggtaag tgcatgatca
     2881 tcaatgacaa tggcagagag tatgatacag aaaagattga agagtattca tcttatactc
     2941 agggattaat taaacgtttg atatacgttc gctatgtagg tattagggat ctgttatcag
     3001 ataactgttg tagtaaatac aaagtgaatc aagtaagaga agcgttgaat aaagataata
     3061 acgtcgaaag aataaaaaat gtttttggat atagtattga agagattaat tattacattg
     3121 acttcgctga agctttcatt ccgatggtga gataaccccc ccttaaaata aatcgcaaat
     3181 tttttggggg tgatgaaacg gagggggctg tcaggaaaag agattttttc gaactttatc
     3241 atgaaaggag ggctaaaatg tttaaaaacg aattgtctca aaatcgctac agagaaaaat
     3301 tacgccgctc tttaataagc caattggaaa gtcagaaaac aaatattgag ccattcttag
     3361 ataatgttga tcgttatatc agtttatggg aaacggcgat atcactggaa gaagatatat
     3421 ccgagaacgg cattagactg gagaatggta aaaagaatga atcagtagcg ttgcttgttt
     3481 ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc cattactcct gaattggtag
     3541 gtgaagcaaa tgaatcaatt cctgagttat aagcatattg aaaattggtt caaagctata
     3601 gaagaaggca ctatcaaggt atgcaaagag caattattgc taaaaaatta tctagaagaa
     3661 agagtcttta ctagagaaga tatttacttc gataagcaga tggtagagga ttcaatcaat
     3721 ataccagcac aatactttcc attcgaatta attccgtggg aaaaatttct acaatgtttt
     3781 atttatggtg ttcgatggaa aaaagataaa acactagtgt tcaatagata tctttcatta
     3841 atgggacgtg gtaatggtaa aactggtttt gcttcttgga acaacttctt tctactaacc
     3901 gctaaacacg gtattaaaaa ttatgatatt gatatctatg ccaataatga aagccaagca
     3961 aagactagtt ttgatgatgt atttaaagta attaaagatc atcctgattt agataaaaaa
     4021 gtatttaaag ctacgaagga agttattcaa aatatcgcta caaacagcaa acttcgttat
     4081 aacacggcaa atgctagaac aaaagatggg aagcgaccag gtgcaaaccg ctttgatgaa
     4141 attcacgaaa atgaagatta ttcaatgata aatgtggcta cttctggtgg tggtaaaatt
     4201 cgagattata gagaatttta tgatacaact aatggtcatg ttcgtggtgg tccgcttgat
     4261 gacattatag aagaatcaaa aatgattctt tctggagaac ttggaattga caaggatgga
     4321 gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg ataacgataa tgaagttgat
     4381 gatcccgaca tgtgggaaaa agcttgtcca actattaatt acaatgcaga tttaaaacgg
     4441 aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg
     4501 accaaacgaa tgaacagacc tatggaagat acacgatttg ctgttgcttc atatgatgat
     4561 gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat aggaacagtc
     4621 gattttgctg atagacgaga ttttgccagc gttgggttgc taggaaaata cgataaagat
     4681 gtttatttta cacaacatac ttttatccac gaatcagccc ttcgattaca aaacatcaaa
     4741 cgagaggtta tagatatttc tatagatcaa ggaaaatcac agatcgttca tggaaaaaat
     4801 atagaagctg attatattgt aggttggttt cttgaaatga gtaataaata ttatattaaa
     4861 aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt
     4921 tttactgtgg aaattgttcg aagcggatct gttacacatg gtatgttaaa agatctggtt
     4981 gatgaccttt ttattaatca acgtttattt tttggtgacg atgcgattat gcgttggtat
     5041 tgcatgaatg tatatgaaga gcatatttct aatggaaata tacgctatga aaaaatagaa
     5101 cctgaaacta gaaaaacgga tggctttttt tcattccttc atggtttgaa ttttttagat
     5161 gatatttatg attctgctcc tgtaacagtc acaaatagct cagtagaaaa tacaggaact
     5221 ggatttactc ctctagtatt ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa
     5281 ggcggtagga tacttcacaa aaaaagcaac ggttccttta gaagaatact tttgtaaatt
     5341 gcaagttgat tttgtgtatc gaaaatttgc gattgaaact tgtattgatt tgattgcaaa
     5401 tgcgatgagt aaagcggaat tcaagtcata tgaagatgga aaaaataaaa agaatgatct
     5461 ttactatagg ctgaatgtag ctcctaataa gaaaaataat gcaacagaat ttagaaaaaa
     5521 actgatcagg agattaatat tctacaatga agtattgatc gtttctccgt ctaataattc
     5581 tagcgaaata tttattgcgg atagttggga tgtcacagaa tatgcattga aagatgatgt
     5641 gttttctcaa gtgcaaatta acaacatagt ccttgataga gaatttctag aaagtgatgt
     5701 tatctatata aaatacgcag atcaacaaat taggcaacta gtcgatgcgt attatcaagc
     5761 gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag cgctctaacg ctcgtagata
     5821 cgtactgaaa gggaatttat tccgaccgca agacaataca acacaagatc aaatcaataa
     5881 aatgatgaca tcacaattta aggcttttat ggaagctgat aatgcaggtg cggtatttca
     5941 attacaaaat gagtacacat tagaagattt cagcggaaac ttccaaagca attcaagaga
     6001 tataaaaaac ttaatagacg acatctttga gatgacagca gcagcgtttc acgttccgaa
     6061 aaacctacta aagggagaca tgagtgggtt atcggatcaa gtggacgctt ttttaatgtt
     6121 cgaaatcata ccgattgctg aacttattca ggatgcgttt aacgctagtc tctatgaagt
     6181 agaagaatac ttgtcaggga attttgtacg tgtggataca actatgatca agattactag
     6241 cttcaaagat ttggttgacg ctattgatgt aggcattaga aatggagtat t
//
LOCUS       DAJSVF010000122         6111 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSVF010000122 DAJSVF010000000
VERSION     DAJSVF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.127"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(324..809)
                     /locus_tag="ON550_002614"
     CDS             complement(324..809)
                     /locus_tag="ON550_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955639.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(948..1193)
                     /locus_tag="ON550_002615"
     CDS             complement(948..1193)
                     /locus_tag="ON550_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR3955640.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1386..2186
                     /locus_tag="ON550_002616"
     CDS             1386..2186
                     /locus_tag="ON550_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3955641.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2205..4073
                     /locus_tag="ON550_002617"
     CDS             2205..4073
                     /locus_tag="ON550_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3955642.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNIFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4066..4557
                     /locus_tag="ON550_002618"
     CDS             4066..4557
                     /locus_tag="ON550_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR3955643.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4545..5099
                     /gene="srlA"
                     /locus_tag="ON550_002619"
     CDS             4545..5099
                     /gene="srlA"
                     /locus_tag="ON550_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR3955644.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5117..>6111
                     /locus_tag="ON550_002620"
     CDS             5117..>6111
                     /locus_tag="ON550_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR3955645.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2163 a          968 c         1224 g         1756 t
ORIGIN      
        1 aatttgaaaa agggatcatt aaaataataa gaagaaaata tgtcaaaagg taaaaaagta
       61 aattcttcta tccaaatata gaaaaattga atctagaaaa ccgcccgaca ataaatttca
      121 ttaactcagt tgaccgaaaa tgaagtagat accaaaaatt tatcaagaga gcaagtagag
      181 aagtgagtat attcataaga gaaatagctg taacgaatta ttaggttaat gcaaatgctt
      241 atttatactc cgtataactt tctcagaaaa aatgatacca ataatgactt gctagtactg
      301 taaatccgta taatatcatc taatcaaaat ggtgttccat ttgcatctgt catcgtaatc
      361 gatccttcgg gtgacactcg ataaaaacca acgcttccag atcctccttg ggaacggatt
      421 tgtttgctgg aagctgtgaa caaataatct cgatcaattt tttgataaaa gctaataaca
      481 atatcgtcac tttgtaattc ggaacggtgg gcaataagaa atagaagtgc tttgtcagag
      541 ttttctaatg gaagagaagg tacagtgctt tcttgcattt cagatgttga agaaatgcta
      601 aacgactgaa tacttgattg gctatgttgc gaatctacat taccagtaga agtattagta
      661 atggaagtag tagaagtact tttagttgaa ccaacctcag ttgttgaaat ggctgctgaa
      721 gattctttaa cagaattctt tgttttatat ccacaggcag aaaaaagtat tacagttccc
      781 aacaagcctg cgtaaatccc tttatgcatg atagctcctc ctaaaataga ttatctttat
      841 tcagtatatc acttaaacaa agaaaagcag atggtttaaa atgaaaaaac cttcccaata
      901 aagggaaggt tcatgaagat aataactttt ttaataatta tagctttcta atatctttca
      961 tcaacttttc tagtaatcat tgggaaaaat ttcaagcagt agatcgcaca aatacctacg
     1021 acgatataaa tgatcttcgc aaaaatagtt gttgaaccac cagcaatcat agctactaaa
     1081 tcaagttcga acaagcctac caacagccag ttcaaccctc ccacaatcaa taaagttaat
     1141 gcaatagcat ctaagttttc attagaattc tcctttctta aatgataaaa caattatata
     1201 tcaaaaagag aaacattgct tatataacgc tcaggaattt tagcaatcta tgcaaaaaga
     1261 tgaaaatttt ttttgctctt ccattagtac acatttttta gctgaagctg taaatttcat
     1321 gtcttaattt tttttagtga gcttttatac tggatttgta agaaaaaaga aggagaggat
     1381 cacatatgtc ggattggttg gatattgcag gaaaagtagt aattgtaact ggaggttcct
     1441 caggaattgg aagaagtatc gtcgaaaatt tgttgaagca gaatgcacaa gtagcgaatt
     1501 ttgatgtaac tgaatgccgt atacagcatg aaaacctact atctttaaaa gtcgatgtgt
     1561 cttctaaaac ggacatagaa gaagggatat ataaagtaat gaaacatttc aagacgatcg
     1621 atggtctagt caataatgca ggaataaata ttccttctct tctaatagat agaaatcatc
     1681 caaaaagcaa gtatgaatta tcagaacaag tttttgataa gatgatcgct gtcaatcaaa
     1741 aaagtgtata tttgatgagc caagcagttg gaagaatctt ggtgcaaaag ggatcaggag
     1801 tgatcgtgaa tctttcttct gaatcaggat tggaaggatc agaaggacag agctgttatg
     1861 ctgctacaaa agcagcgatg aacagtttta ctcgttcttg ggcaaaagag ttaggaaaga
     1921 ggaatgttcg tgtggtaggt gttgcaccag ggatactgga agaaaccggt ttgagaacac
     1981 aagaatatga agaagctctt tcttatacga gaggtataag cgtcgaacag ctgagaaacg
     2041 gttacagtcg aacaagtacg atccctctag gaaggagtgg taagctgcaa gaagtggcgg
     2101 atctagtctg ctattatctt tcagaaagat caagttatat cactggagtc acgactaata
     2161 tttcaggagg taaaacaaga ggataaagga taggggggac gcacgtgacg ctagtaaaca
     2221 gatggtacca attattgaaa ttactagtca ctcacaagaa agtttcaatc caggaagtgc
     2281 aaaaagaaat caaaaccagt aatcaaacca taaaaaaaga tattggggag ttgaatgaag
     2341 aaattgccgg tattgcaaaa atcatccaaa aaaacaaaca ttttgaactt gaaatttatg
     2401 attttgattt ttttgatgag atcatgcaag gaaaattgaa aacagccagt gatttcaatt
     2461 cggcatccaa aagaatcgct tatttactaa ataggctgat cgaaacagag gatttcttac
     2521 gtatggatga tttgtctgaa gaagtcggtg tcagcagagg aacagtggtc aaggatctaa
     2581 agacattaaa agagatgatc aatgattttg atgtaaagat tacaggcaca ccaaataaag
     2641 gactaagaat aataggagat gagctagaat tacgactact tttattttat tttgtttctc
     2701 cctacttttc agagacgaaa acagaacagt ttctagaaga aaaacaagca atcaaacatt
     2761 ttcagaagaa gacgaatgct agtaaacaag aaatcaccct tttaacgaaa gttatcggta
     2821 tttctttgaa tagaatctct agtaactatc tgttgaagaa gcccatcaat cgttacagtg
     2881 ggtgttttga atatatccaa gaattcaatg aactgatcaa tcagttggaa gaaatctatg
     2941 aactaacttt gagccaatat gaaaaagatt ttatcagttt tcctttgaac atcatcaaca
     3001 caagtataac gatgataagg aaagctaact ctgatttgcg cttatatttc gaccccatga
     3061 tgatgcgtat tcgacgatta gatctcattg atctggatga gtccttctta tatcaagaga
     3121 tgaaacagca cctctcttta ttgataaatc gggtaatttt tcgttatcga ttgactgatt
     3181 tattttatgg tgagatagaa aaaaagtatc ctttttctta ccaaatcgct agcgaatgca
     3241 tacaagcatt agaagctatc ttgcaacata agatctctaa agtagagatc agctacctaa
     3301 cgttgtattt ggagttagca tttcgatatc aaaaagagca tttttcctct aaaaaagtag
     3361 ccattgttca tacgacagga aaaggaacag cattaatgat ccaaagacaa atcaaaagga
     3421 tattggggag ggatatacaa ataacacatg tttcagaaga acaatatgaa aaagtagatc
     3481 taaacaaata tttcgctgtt tttacaacga ttccgttaaa aaatgtaaat gaagagacac
     3541 cggtgatcca tttgactaat atttttgatg actcttggtt aagggcacaa tggcaaaaag
     3601 caaaaaaaaa gcgattagcc gggtttcatt ctactgtttt tttctatcaa atatggaaag
     3661 aaaaaaagac gtatcaagaa tatttaaaca aattagtcgc tcctttgatc agaggctatt
     3721 atgtggatga agaatttgaa aaaaggatct tgaaaagaga atatcagcaa agcacatttg
     3781 ttaacaaaga atttgctttt cctcacacaa ccaaccagct atctcaacac attgttttat
     3841 cagtagggct aataactgat caaggtttag cagaaacagg aggaaatgga gttatttttt
     3901 tatttgccat acctgaagaa atgacggctg aagttgagaa tgaattattt gagctatacg
     3961 atatcctatt tgaactcata aaaatgggaa tagatgagtt tattgaatta gcaccgacac
     4021 aagaatcatt tttaaaacta atcaaaagta aaggagagag acttcatgaa tgatatgtat
     4081 ctattcatag ttttcgcagc ttcagcttat gtgctgcaag cagttatcag ttggaagcaa
     4141 ctaaagcatt tcaatcaaac atatagcgaa ctgcgaagaa aaggaaaagt agcgattggt
     4201 aaacgacctg ggaaaatccg ttcaggcacg attgttatgc tagctataga cgatcatgat
     4261 caagttttag aagtcagaaa aatgcaaggg gttacatctt tggcaaaatt tcattcatta
     4321 cctgtctatg cgggtgaaga tatccattac atcgatcgtt accatccctc agttcgagcg
     4381 gaagatcgtt tgacacaagc agctttagaa aatgccagag aagtatactt acgaatagag
     4441 gcaggaaatg aaaaaagtga acaaccttca ttgcctttta ttgctatgct caataatttt
     4501 cggatatgga aaagtacgat caaagaaaaa aggagtgaca aacgatggat atcataacaa
     4561 aatttgctga gggatttatg aggctgttcc aaacaggtgc agaaacattc ataagctgga
     4621 tgacaggaat cgtaccagtt gtcttgatgc tgatggttgc aatgaatacg ctgattgctt
     4681 ttttaggtga agaccgagta acgaaagtag ccaaattatc agcgaaaaat ccgcttactc
     4741 gttaccttgt gttaccgttc ttatctgctt ttatgttggg aaatcctatg tcatttacaa
     4801 tggctcgttt ccttccagaa tactacaaac caagctatta tgctgctcaa gctcaatttt
     4861 gtcatacgag caatggtgta ttcccccata ttaatcctgg agaattgttt gtatggatgg
     4921 ggattgctca agggatcgaa accttagggc taaattcgat ggagctggct atccgatact
     4981 tgcttgtggg actgctgatg aactttattg gtggttggat aactgatttt actactggat
     5041 ttgtatgcag acagcaagga atcgttctta gcaaaaaagt agagctttca gttgattaga
     5101 gaaaggagag ttttaaatgt atggtgatat taagatcgta agaggaaacg gcgggtatgg
     5161 aggtcctttg attattaggc cgacaaaaga taaacacaag ttcatctatg tcacaggtgg
     5221 aggtgagagg ccaatgatcg tagataaaat agaagaactg acaggtatgg agtctatcaa
     5281 tgggttcaaa acatcaatac ctgatagtga aatcgcgctt gcaatcgttg actgtggagg
     5341 gactcttaga tgtggaattt atcctaaaaa agggatacca accatcaata tcgttcccac
     5401 aggaaaaagt ggcccattag cacaatatat cactgaagat atttatgttt cagcagtagg
     5461 gttagaacaa atacagccac taatggaaac tgatgataac aaagaaacag taaatactgc
     5521 atctccagat aaaaagtaca aatatgatac ttctaaaaag ataaccgagc aaaaagcaga
     5581 gcagcaaaac tggatagcaa aaattggaat gggcgcggga aaagtagttg ctacattcaa
     5641 tcaatcagca agagaagcag tagatacgat gatccggaca gtgatccctt ttatggcttt
     5701 cgtttccctt ttgattggtg tgatacaagg ttctgggatc ggtaatgtat ttgctaacat
     5761 tatggcacct ttagcaggaa atgcagttgg attaacaatc attggcttta tttgttcact
     5821 gccattttta tctcctttgc ttggtccagg ggcagttatt agccaagtga ttggaacatt
     5881 gatcggtgta gagattggga aaggaaatat tcctccccaa ttagcattac cggcactatt
     5941 tgctatcaat actcaaaatg catgtgattt tattccagtc ggtctaggtt tggaagaagc
     6001 aaaagctgag acagtagaag taggtgtacc atcagttctt tattcaagat ttcttaacgg
     6061 catcccacga gttatcattg cttgggtagc aagttttgga ttatatagct g
//
LOCUS       DAJSVF010000123         6068 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSVF010000123 DAJSVF010000000
VERSION     DAJSVF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.129"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            832..1566
                     /locus_tag="ON550_002621"
     CDS             832..1566
                     /locus_tag="ON550_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3955646.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ON550_002622"
     CDS             1582..2751
                     /locus_tag="ON550_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3955647.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ON550_002623"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ON550_002623"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR3955648.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ON550_002624"
     CDS             3841..4767
                     /locus_tag="ON550_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR3955649.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ON550_002625"
     CDS             4786..6036
                     /locus_tag="ON550_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3955650.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAJSVF010000124         6021 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSVF010000124 DAJSVF010000000
VERSION     DAJSVF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.130"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            589..3228
                     /locus_tag="ON550_002626"
     CDS             589..3228
                     /locus_tag="ON550_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3955651.1"
                     /translation="MEEYKQTIEEVRKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESKQLVVGDILILESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFISGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3396..4094)
                     /locus_tag="ON550_002627"
     CDS             complement(3396..4094)
                     /locus_tag="ON550_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3955652.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4312..5457
                     /gene="tgt"
                     /locus_tag="ON550_002628"
     CDS             4312..5457
                     /gene="tgt"
                     /locus_tag="ON550_002628"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR3955653.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5554..5934
                     /gene="yajC"
                     /locus_tag="ON550_002629"
     CDS             5554..5934
                     /gene="yajC"
                     /locus_tag="ON550_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR3955654.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
BASE COUNT         1908 a         1105 c         1314 g         1694 t
ORIGIN      
        1 aaaaaagctt tacaaagatt ttatgacaaa gaaaaagccg agctctcgat aagctcggct
       61 ttcttgtacc tcggaaggat tcaaataaac agaaatgatt gtaactttcc atcatacatg
      121 caaagcacgg tgttgaattt cttcatgtta aacaaaaatt ttgttttcgt tacatcttca
      181 acatttgaaa catctcttgt tcctattcaa ttttcagacg tttacgttat tcaattgtta
      241 cgtatcgcat tacttcttgt ttcggatact tcgtgatcca aacatccata cgcattcact
      301 atttataggt ttgtactcta cacgcatgtc tgggtgaaac ctgtacttct aacaactttg
      361 tttgcttaca ctgttctatc gagaaattct tctgtctatt tgatggtatc ctccttcctt
      421 acatctatat aatagcatcc tttggtagcg tttgcaaata taagcacttt aaattttacg
      481 ctttcttaac aaataatcga taagcccttt atttgaagta aataaaaaaa ggatgttata
      541 gtaaaataag tacctttttt tgtacgaaga ttttaaagga ggaataggat ggaagaatac
      601 aagcagacaa tagaagaagt aaggaaggtt acagaagcag atccagatac tgggttgtct
      661 tcacaagaag tggatgaacg gcgaaaaaag cagggattga ataaattcga tgaggcgccg
      721 aaagaatcga tgatcaaaaa attctttcga agcttgtccg attttacaac gatcatcctt
      781 cttgtagcag cggtgatttc cttttatacc gcatttgcta cagaacatgg ggatttattt
      841 gaaggactgc tgatcatcgc tatcgttgtg atcaactcgg tattggcgat tgtccaagaa
      901 ggaaacgcag aaaaagcact ggaatccttg caagatatga ataaacaaac ggcaactgtc
      961 atacgagacg gcaaagtgac cactgttgag tcaaagcaac tggtagtggg tgatattctt
     1021 attttagagt ccggtgatgc tatttctgcc gatgcccgtt tgatcgaagc atcacaatta
     1081 cgtgtagaag aatcggcgtt gactggagaa agtgaagcag ttgaaaaaga tgcagcattt
     1141 attgctgaag aagatgagtc attaggtgac caacttaata tggtatttaa aggatgtacg
     1201 gttgctgcag gtcgtgggaa agcgatcgtc actgccattg gtatggctac agaaatgggt
     1261 aagatcgcgg atcttttgaa cgaaaatact atgcaaaaaa cacctttaca ggtccgattg
     1321 aatcagctag gcaaacgaat cagtatgatc gctttagcgg ctgcggcttt agtatttgtg
     1381 attggtgaac tgcaaggaga accactcctt gagatgttca tgacttcgat ttccttggct
     1441 gttgcagctg taccggaaac cttgactgtt attgttacgt tgacacttgc atacggtgtg
     1501 cagaagatgg cgaggaaaca tgcgatcatt cgacagttgc cagcagttga aacgctagga
     1561 accgctaatg ttatctgctc agacaaaaca ggaacgctga cacaaaatga aatgcgcgtc
     1621 cgccgggtat ggagcaaaga agatgaagta acgaatatcg aagattcaat gacgaacagc
     1681 gcgatggaaa tattgaaaat ggctgcacta tgtacagatg tgacggtcga gcaagaagat
     1741 gacgaccttg ttatcaaagg taatccaaca gaagtagcca tcgtaagggc tgttgaagag
     1801 aattatcata caaaagcaga attagaagaa aaatatccac gagtaaacga gcttcctttt
     1861 gattctgaac gaaaaatgat gacaacagtt caccaaatgg gcgaaaaata catctcaatc
     1921 accaaaggag cattcgatgt tcttgctccg cgctttattt caggcgatgt agaacaagcg
     1981 gcgatcgtta atgatcgctt tggtaaacgg gcactgagag tgattgctgt tggttatgct
     2041 acttatgacg aagaaccgca agatatttct tctgaagcac tggaaaaaga cttacgtttg
     2101 attggtctga tcggaatgat cgatccacca cgtccagaaa gtaaaggagc aatcaagcga
     2161 gcgaaaaagg ctggcatcaa aacagtcatg atcactggag atcatgtggt aactgccagt
     2221 gccatagcga aagaattagg tatcttgaat gatccgtcag aagcactctc aggatcagaa
     2281 ctgcatcaat tatcagacga agaattagat gcgcgtgtca aagctctctc tgtctatgct
     2341 cgggtaacgc cagaagacaa gatccggatc gtcaaatctt ggcaacgtac gggtgcagtc
     2401 gtagcgatga ctggggatgg tgtgaatgat gcaccagcgt tgaaagcaag taacgttggg
     2461 tgtgccatgg ggatcacagg gacagatgtg gcgcaaagtg ccgcagatat gattttgacg
     2521 gatgacaact ttgcgacgat cgtagacgct gtcagtcaag gacgttcggt ttatcagaat
     2581 attcgtaaag ctatcaactt tttgctaagc tgtaatattt cggagatctt tattgttttg
     2641 attgcgatgc tgttaggctg gggagcgcca ttcacagctg tgcaactact gtttgtcaat
     2701 gtagttgccg atggtcttcc aggatttgct ttgggaagag aaccagcaga acacggcatc
     2761 atggatcagc ctcctattcc gaaaaacgaa gggatctttg ctcgaggctt gttgcaaaaa
     2821 atcgccataa acgcaggagt cttcaccatc gtaacgctgt ttggttacta tttaggtagt
     2881 tatgtcgata ctattagccc atgggtagat gcaagtcagc atgtcggtca aactgttgca
     2941 ttcttgatct tagcttattc atcgattttg catgtgttca atgtacgcag cagccaatcg
     3001 atcttcaaag tcaatttagc aaccaataaa gctttggtag aaatggcgtt actggcatta
     3061 gctattacaa cggctgttgc tttgctgccg tttacgcaag aactattcgg cctagtacac
     3121 atcagtttga atcactggtt cttggtaggg attttgtcta tcgtaccaat cgcggtcaat
     3181 gaactgatca agttccatcg attaccggaa gcagaagaag aggaatgatt aactgtttct
     3241 aagttgattt tctttttact ggaactatac aaaaaggagg tgtgacatga atgttgtcac
     3301 acctcctttt tgctaatggg ctgaatgttt cttttactgc ctctcgataa acggtgttct
     3361 cctgccaacc cctcggtcac aagtcacact ctcttttaaa tatcttttgg caattgttca
     3421 taaaatgctg cttttgttgc agaaatgtat gggctgagcc atagataacc aataccagca
     3481 gttgctaatg ctaagatatg ccagccgata aatgaaagat ctaaccagaa aagttgtcct
     3541 ttataaccat ccatcaattt tcgacttgca gtgatcgtat ccagtacttt aggtgtttgt
     3601 cctgtttctt ggacgatatc gtagtaaata aaatagcttt gagaataaga atagctttta
     3661 atgattccag gaataactaa tagcaaaccc cataatgttg taaagatcga gatcagcaca
     3721 gctaagctga tcactccgcc gataaacaaa cctttgaatc cacgaaatgc atctttgaat
     3781 ggttcgatct gatctttttg tccacgtaaa atatcaagat aagtccaaga aataccgctc
     3841 atgaacaaag cgctaatgat gctggaaata attccgccac cgcctcctga actaccgcct
     3901 cctgaagttg tcgcttctga gacgctcgct gatccatcat atatggtggt ggcaaacaag
     3961 ataataggaa tcataataag aagaatagta gcaataccaa tcaatgtagg gattagattt
     4021 agtaaaacag cttgtcccca acgtcctttt agactctctt ttgcttggcg ttttaattct
     4081 gcagaagatt gcatgatttt tccctccttt tctttaataa caccaatata ccatgtatca
     4141 aaaaatattt ctgtcataat gtttttcttt tttttgttat tcataaaaaa aggataaaaa
     4201 aaagaatatt aaataaagta gaaggaagcc gagggaactt gcaaaaagca cttgaaacga
     4261 ttatgatggt gggagttgtc aagatatttt atatagaaag tagggaaatg aatgacgcaa
     4321 cctgccattc gctatcgttt aatcaagaaa gaaaaacata ctggcgcgcg tttaggggaa
     4381 ttgatcacgc cacacggaac tttccccaca ccaatgttca tgccagtagg aacattagct
     4441 acagtaaaaa caatgtcccc agaagagttg aaagaaatgg gtgctggcgt tattttaagt
     4501 aatacatatc atttatggct acgtcctggt gaagacttag tagaagaagc tggcggactg
     4561 cataaattta tgaactggga tcaaccgatt ttaacagatt caggcggctt tcaagttttt
     4621 tcattaagtg atatgcggaa tatcgaagaa gaaggcgtgc atttcaaaaa tcacttgaac
     4681 ggttcgcgta tgtttttatc accagaaaaa gcaatcaaca tccaaaataa attgggctct
     4741 gatatcatga tgagttttga cgaatgtcca ccattcgatg aaagttacga ctacgtgaaa
     4801 aaatcagtag aacgtacgtc tcgatgggca gaacgaggat tgaaagctca tgctaaccca
     4861 gaccgtcaag gattatttgg aatcatccaa ggagcaggat ttgaagatct tcgtcgtcaa
     4921 agcgcgaaag atctagtcgg gatggatttc cctggttact caatcggcgg gttatctgtc
     4981 ggggaaccta aatcagaaat gaaccgtgta ttagaattca ctacaccgtt gatccccgaa
     5041 aacaaaccac gttacttgat gggtgtcggt gctgctgatt cattgatcga tggtgttatc
     5101 cgtggagtag acatgtttga ctgtgtcttg cctactcgaa ttgctcgaaa tggcacatgt
     5161 atgacttctc aaggtcgtct agtagtgaaa aatgcgaagt atgctcatga tttccgtcca
     5221 attgatgaga aatgtgattg ttatacatgt aaaaactata cacgtgccta tatccgccat
     5281 ttgatcaaat gtgatgaaac atttgggatt cgtttgactt cttaccacaa tttatacttc
     5341 ttgttgaatc taatgaaaca agtacgtcaa gcaatcatgg atgataattt gcttgaattt
     5401 agagaagcct tttttgaaga atacgggttc aataaagaaa acgcaaaaag cttctaaagg
     5461 aaaatctttt aaaaattaag taaaaaacta gtgttagcag catttttctg ctaaagtgtt
     5521 ttttgtagat acagaattag tgaggtgaaa aaaatgggaa cattaccaat gcttgtgatg
     5581 tttatcgcgc tattagcaat gtggtttttt atgtcacgtt cgcaaaaaaa acaacagcaa
     5641 gaacgtcaaa acttgttgga tagcatgaaa ccaggtgatt cagtcgtaac tatcggcgga
     5701 ttacatggag ttgtttcaga aatcgacaat gacaaacgga cagttgtttt agactgcgaa
     5761 gggatcttcc ttgagtacga tcgtgcagca atcaaaacgg tcaaaccagg gactgtcgta
     5821 acaaatgatg caacaactac tgttgaaaca acagaagaac cagctgagac agttgaagta
     5881 aaaactgaaa caacagaaac gctggaagag acagaagaga agaaagaaaa ataagtatca
     5941 aagcttattt aaagacgagg tagggcagaa atatgtccta cctcattttt tattcaaaaa
     6001 agaaagagat aagagtatag g
//
LOCUS       DAJSVF010000125         6019 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSVF010000125 DAJSVF010000000
VERSION     DAJSVF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.131"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            584..637
                     /locus_tag="ON550_002630"
                     /pseudo
     CDS             584..637
                     /locus_tag="ON550_002630"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            641..1240
                     /locus_tag="ON550_002631"
     CDS             641..1240
                     /locus_tag="ON550_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3955655.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            1256..1732
                     /locus_tag="ON550_002632"
     CDS             1256..1732
                     /locus_tag="ON550_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955656.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            1749..2651
                     /locus_tag="ON550_002633"
     CDS             1749..2651
                     /locus_tag="ON550_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955657.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            2724..2915
                     /locus_tag="ON550_002634"
     CDS             2724..2915
                     /locus_tag="ON550_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955658.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            3079..3666
                     /locus_tag="ON550_002635"
     CDS             3079..3666
                     /locus_tag="ON550_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955659.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            3666..4565
                     /locus_tag="ON550_002636"
     CDS             3666..4565
                     /locus_tag="ON550_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR3955660.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            4565..5479
                     /locus_tag="ON550_002637"
     CDS             4565..5479
                     /locus_tag="ON550_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955661.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            5531..5746
                     /locus_tag="ON550_002638"
     CDS             5531..5746
                     /locus_tag="ON550_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955662.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         2305 a          769 c         1210 g         1735 t
ORIGIN      
        1 accttggtca caggcagaaa ttgataaagc agtaaaattg aaccgattat caaaatttaa
       61 cgtattagga aatgcctttt aaaaatcgca atacacaaac agaaaaatac caaaaaatta
      121 gcaatgaacg taggtaagaa catgacaata aataataata accctaaaaa atataatgct
      181 cttgtccaat gctatatcga aaacaaaatt acaatgcaag aagtcgctga tgaattaggc
      241 aaatcaaaga gcctagtatc acacgatttt aagcacaatg tttttccgaa cccgattgag
      301 ctgaggaaaa aatttcaaaa gagagtttat caagaaatag gtaagtggat tgatgaaggg
      361 aatgcaattg accctttaat tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa
      421 aggttaattc gtcaaggcga tattcaagct aaagaacctg ttttgacaac caatgatttg
      481 gctaaaataa atcttcataa acaaattatt gaagactttg aaaaaaatct ttcaacaact
      541 gaaattttaa aaaaatacaa tattccgaga ggaaagctaa catcgactct ttttagagaa
      601 tatggagaat cggcagttac taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa
      661 tagtagtcga tacctagtag cgactgtagt ttttgtttgt gtattgattt tcctgtttga
      721 ttttttgcag attggaaaaa gcataattgg aaatacattg agtgcaaagg aaatggttgc
      781 tttaggtggt ttgtatgaag gacacttgtt cccgaatata ttcatttcta tcgtttctca
      841 tattaacttg taccatttaa tttctaattt attgttctta gtagttcttg aagaagcact
      901 gaatgatcag tatgagtggt taacattatt gatcgtttta atcatatcag gatttctagg
      961 aagcttagtt ccttttctgc tacacagcaa catcgtaagt atggggctta gtggaggtat
     1021 ttttggatgg attgctttat ttgcgattgc atacatccgt tttgatagca agaaacattt
     1081 attactaagt gtttttgtat tattgataat cggcatttta ttggtatcta cttttgaatc
     1141 aggcgaggca aatgtcttgt cacatgtaat cggatttgtc tgtggaatga tcttgggatt
     1201 tattctaaag aacgtttcat tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc
     1261 aaaaagaaaa gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca
     1321 aatactgtta agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat
     1381 gtacgaacaa cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa
     1441 aatctatgaa gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag ctcaacaacg
     1501 gatacttgaa tggcttaata cgaatactga tcaagaaccg ttaagagtga ttagtaatct
     1561 ttttgaattt ggaacgaagc attggtttaa tgctgtagag aaggatgctg acttacaaga
     1621 agcgtttgat acgctaagta aggatagcaa atccatgcga gatttattga ttacttatct
     1681 aaatgagcat gagtttgaac aaacgaaaac caaaaagaaa aataaagaat agaaagttgt
     1741 gatgaatcat gctattaacc ctgttaggtt atttatgttt aattcttgct ttttttgggg
     1801 caatcgctat aattattggt caatttcaaa caaataatcc gattatgcgt aacataggca
     1861 ttggaattgt gattgtaacc tttattgtgt ttaatgccac taagattttt ggaagtaacg
     1921 ataaacaatt tggtatatcg ggagttaata atagttatgc tccagattct ttgggaaggg
     1981 tagtaataga tggaacattt gaaaatgtaa cgagttataa aaatgtttcg ctgacagtga
     2041 aggaagatgg caagaaagca tcagacgacg ttctcgctac tattaagccg tctcaatcac
     2101 aatttgaagt gttttataca gttaaagaga atagccctgt acacaagctg acattggttt
     2161 ttaacgttga tggagaagaa tactataaag atgtaaaaat aaataaaaca tcaacgaaaa
     2221 agattatcga atcagcgaaa aaagaagtag ctacagaaga aagtacaact gaatcaagtt
     2281 ctactgtcga agcggaacta ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt
     2341 ttgaatctgt ttctggttct atggctgatc ggtttaacac tgaagtcttc aaagctatca
     2401 aaactgttaa attggatact gaaaagcaaa tgattaatgt taccttattc aattcagttg
     2461 aatctatgga tcaggataaa aaagttacgg ttgcaagaga actaggaaaa agagtaattg
     2521 gtttgtatcg tggtactgct aaaccagatt cagacggtgc agatttgcca gttcatgtag
     2581 tttatgaaga cggtaaagtt tttggagatt ctacagcaaa cgaagcatta acgattaaat
     2641 tggctaaata gccagaaagt tataataaac aaatggtgtc acaccaataa tatataggtg
     2701 tgacaccttg gagatgatag ctgatggtag taaataaaga aaaagttaga aagtatgtga
     2761 ctttaaaaat agcttctgtt aaatatttag aaaagcgagc taaagaagaa gaaagagcaa
     2821 gaggtaagaa agtaacagtt tcagagttgt tagaagaatt gatcgagaat gatcaaacaa
     2881 taagacgtgc atttactaat aggtctatca gataacaata taattaataa aaaagaaaga
     2941 gtgatttggt cttagactaa atcactcttt ttatataaag tttctttaca ctcattccga
     3001 ataatgttaa aattctttat atgatatata gttccttgtc agaggaagag ataaccagca
     3061 atattggagg gataataagt ggaaaacaat aatgatatta gacattttag cattggggaa
     3121 agagttacaa cagtaagcga attatcaaag gattatagag ataaagaggg aacgattatt
     3181 tctcttaata agaatcgaag aggcgtttgg caatatgttg ttgatttagg tgatggcgtt
     3241 acagttgagt gtaaagatag tcaactgatc agaaataatg gcagtcattc cattgaaaaa
     3301 gcaattcttg tatacccaaa agtatcaagt aagatcatga atgactggtt gcttggaaaa
     3361 tttggtgagt acaataaaaa agaaatcgca gaagtgatcg gcaaatctta tccaacagta
     3421 ttagggtatc taaaaaagat aacgagtaaa gaagcaaaaa ttgaggatgt tttaacagta
     3481 agagagcttc aatcattaat cgagtatcgg aataaacaaa taacaaatcc tgagtacctt
     3541 agaaaagaag atggtctatt gaaacaattg gaacaagtaa ataaaatccg gaagatgcaa
     3601 aaggtagctc ctttaagcct tgataccttt aaaaaaatct atttgaaaga gatcaaaaaa
     3661 gactaatgtg gactgtaact ttaccgttcc aacctgtatc tgcaccaaga ccaagtgcaa
     3721 gaaggaatgc tgctgatgaa actaatgacg tagcagagaa aaggatttca acctactatg
     3781 ataaaaagta tcttgattat ttgaaaaaaa taaagagctt tatcgaagat caagatttat
     3841 tagatgatga attttactcg attgtaaatg atcctatggg cgcaattatg gaagtggact
     3901 tttactacca gataccaaaa agctataaaa aagtctataa cattatgaag acaacagcgc
     3961 cagatttaga taatttagta aaaagtgcta tggatggtat ttttaacatc tcagctatac
     4021 gagatagcca tatcacaggc ttaattgctt ttaaatataa tgtggcgaac gaaggaaaaa
     4081 cagttgtgag gatcaaacga tattcagaat ttgagtggga tacttctgaa ggattgaacg
     4141 gagatatatg ggaagctaca tttgatttta aaccagtagc aacgccaaga ccaaagtcta
     4201 aatttagagg aaatgggatt atcacttact atccaaaaga gtacaacgac tatttggaaa
     4261 atatcaagaa tacgcttaaa gagaaagatt tggtaaatga tcaactgaaa aaggtaatga
     4321 atgctcctat gggggttata gctcagatag attattttta ccaagtgaga aaagataaga
     4381 aaaagcttga ttcactgatg cgtacttcac agccggatat tgataattta gtcaaaggaa
     4441 cattggacgg aatttttaac aaggaaatag gcatcaaaga tagcagggtt gtagggttaa
     4501 ttgcttttaa atacaatact tttgaaaagt cgaaaacaaa cgtgagacta caggagatgg
     4561 gctgatgaat aataatgaaa aaattcttca tgtacttgat agctttgaaa taattcaaga
     4621 agaactaaaa aaatatcggg atgttttgga acaaagatat gattttgtca atcagcaaaa
     4681 gagtaatcac atggatttca ttctcaatat gaatgacttg aagaagaaat tagttgaacg
     4741 aaaagaacaa gaaaagttga tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa
     4801 tgcaatggaa ctgaatgaag agcgcagggt tttagatcag ctgttagaac aattattggt
     4861 gatgtttcaa aaaggacgaa tagatgagga tttaattgaa gaaggattga gaaaatatcc
     4921 ttcaaactca ggtatcggaa tagtgctgaa agcgattgat gaagaagaaa ttgaagcttt
     4981 cataccgcca gaggattttg aatcggcaat ggaatatata aaatattata gccaagggat
     5041 cactgctttt agagagtttg atcctgaaga tgttattcat gacttgaaca acctaaaaga
     5101 atggtgtgaa agttatggag tcgatgattc cggattggac tacttgatca gtattatgga
     5161 aatcgaagaa gagatgccgg ataagcctga tccaacagat attttagaat tgatccatga
     5221 agctagaaat ccaattgcct atatttctag aggatatact gtattggaat actataagcc
     5281 ttatatatca gcgatgaacc atttaagacg tgtattaagg gaaaaacgtg aataccgctc
     5341 agtgttgaat gcaacgaata gattagaaaa agctgtttca gagctagatg cttattatcg
     5401 tgaacattac ctgcaagcag gtggtatgcc aagaaatacg aaagcaaata tcagtaggta
     5461 tataaaaaaa gctgagtagg cggagttagc ccataaaatg gcaaaaggta acctgaaagg
     5521 ggcattttga atgaaaaggg atgtttttga attgtacgta ttagaacgtc tagaagacgg
     5581 catgatggca caagagataa ttgatgaaat tgatgatatg atcagcagat ttggtcaatt
     5641 ggagttgaat gatggaaaca aaaattcggt agtgctaaaa caattttggt tgaacaattt
     5701 ttcgtataag gattttatca aagaaaaggc aaatacattg tactgactaa attcacgagg
     5761 agcagtgatg agagcttatt gctaagagca gttaggctct tttttgtttg tgtaaaatta
     5821 tttgttttat aatacacatt tatacacaaa aaatattgac agatagtaca caaatgtggt
     5881 aggatatatt tgtaagcaag ttagattaac aagttaaagg aagagcagtt atgaaaaaag
     5941 aagaactaat agatatattt caaattgttg aacgagcaaa taatatggga attatgtttt
     6001 ttgatcgtat ttctttaaa
//
LOCUS       DAJSVF010000126         5962 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSVF010000126 DAJSVF010000000
VERSION     DAJSVF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.133"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..446)
                     /locus_tag="ON550_002639"
     CDS             complement(<1..446)
                     /locus_tag="ON550_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3955663.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELIT"
     gene            670..1461
                     /locus_tag="ON550_002640"
     CDS             670..1461
                     /locus_tag="ON550_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3955664.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1427..2380
                     /locus_tag="ON550_002641"
     CDS             1427..2380
                     /locus_tag="ON550_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HCR3955665.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2778..3752)
                     /locus_tag="ON550_002642"
     CDS             complement(2778..3752)
                     /locus_tag="ON550_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3955666.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3867..4301
                     /locus_tag="ON550_002643"
     CDS             3867..4301
                     /locus_tag="ON550_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3955667.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4426..5448)
                     /locus_tag="ON550_002644"
     CDS             complement(4426..5448)
                     /locus_tag="ON550_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR3955668.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5840..>5962
                     /locus_tag="ON550_002645"
     CDS             5840..>5962
                     /locus_tag="ON550_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCR3955669.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETH"
BASE COUNT         1874 a         1125 c         1027 g         1936 t
ORIGIN      
        1 gtaataagtt cgtatatgtt ttgcggtgtt tcttgtttct tgtaattttc cacgaatgtt
       61 tcatccatga ataaattcat aaacgcttga agtaatccta cttgttcttt tggttctttg
      121 attcctaaaa aaaatagatt ttctacagtc atttcctgat tatcccccat ttgataaaaa
      181 taaattgggt tcttcagctg cataacaaaa acaaatggct tttcgacata ttcacattcc
      241 gaatgtggca gcgcgatatt taaatactta gtaatcaatc cagtaggaaa ttctctttct
      301 cttgcaatca gtccattcaa ataattctca ttgacgtacc ccattttttc taattgttgt
      361 gcagcgatta aaaaagcttc ttctttattt tttacttcta ccgctaattg aatcaaacta
      421 ggaacgaaca tctctttata tttcattcca acctcctcct ttttgattaa gtttgttaat
      481 ttattgttct actaagttca tctttgtttt acaagaacat tatatttctg ttagtttgtt
      541 ttgtcaacgc tttcaaaaga aatttatttt gttaaatttg atctacttga aaataaaatc
      601 gtttatctat ataattaaga agaacaaagt cgaacaaaaa ttgactttta tgttaaagga
      661 gtaattcata tgctgaaaaa agaaagacaa gaacaaatca ttacattatt agagaaagat
      721 ttttatttaa ctaattcaga aatcgctaac aaattaggcg tatcagaaat gaccatacgc
      781 cgagatgtca ctgaattagc aaaaaaaaat aaagtaaaaa agctatatgg tggcgttgaa
      841 cgaaaggaac cttataatcg agaactatcg actcaagaaa agattgatac gaatgtagcc
      901 gaaaaaaaat atattggtca agtcatgaac agtatgattc ccgataattc tactatttat
      961 gtcggtgctg gaacaacgat cctatatgca cttcctttca ttcaaaagaa aaatctattt
     1021 gtaattacaa acagtttgat tgctttcaac tatttaaaga atcatacaag ttatcggatt
     1081 ttacttactg gtggcgaatt ttctcctatt acagaagagt tcattgggga agttgcagaa
     1141 agatcttttg aaagtttgaa tgtcgatatc tctttcgctg caacaaacgg catatttgat
     1201 aacaatgtga caaccgctca atttattgag ggaaatattc agaacgcagc gctcaatgca
     1261 gcaaaaatca aatgcattgt tgcagattat tcaaaattaa ataaatcaga cgtctatact
     1321 tttagaaaat taaccgattt tgattatcta ataaccgata atcatttatc agaacaggat
     1381 ttcaatcatt attcaaaata cttaacaata ttaaaggagc ccaaaaatga tactgacaat
     1441 cacgatgaac ccatccattg attccattta ttttacaaaa cactttgaat taggtacgat
     1501 gacacgcttt gattctccgg tacgctatgt aggcggcaag ggaatcaatt gcggacgagc
     1561 ttctgctcta ctaggttcat cagttatttt aacagggttt ttgggcggtt attttggcag
     1621 tatgattcaa gatatgctga aaaatgaaaa tttgtttgat ttgcattttt tacctattgc
     1681 agaagaatca agaggtgccg tcacgatcat gcatgatgat gaaacgcaga cagagctagt
     1741 ggaagctggt cctgaaatag cagatactac tatcaaaaaa ttaatgaatg atatcaaact
     1801 aatcagtaaa aataattacc tttcagtcat tacaataaat ggttctgtta acaatccaga
     1861 tccctttttg tatgctaaat tattacaata tatcaaagaa caaatagatc cttccaccta
     1921 tatattaatg gatatatctg gacaacatct ccgtgaagtc ttagcaagta agtataaacc
     1981 gatgttcatc aaacctaata tccatgagtt ttcagatatc atccatcaaa aaattactca
     2041 aaaaagcgac ataaaacacc atctaaaaac aaatccacta tttaaagata tcgatgttat
     2101 catgatttct tgcggctcgt atggtgcagt agtcaaagta aaagaagaat tttggaatgt
     2161 tgagatccct aagattccta tcattaatac aacaggctct ggggatgcca ctgttggagg
     2221 ttttgctcat tcattagaac agaattatcc tttaaaagaa tgtgtaaggt acgcaatggc
     2281 ttgcggcatg tcgaatgcac agcaatcaga agtcgggaaa gttattccag ctgatgtaaa
     2341 aaatttagca aaaagcatta aaatcacaga aatgaaataa atagtacaaa gtaaataatt
     2401 gtttatatga aatgagacac aaagagactg tgacaaaaaa cgtgtgtacc gacccccaaa
     2461 agttagattt tttggtctaa cttttgggag tcgatacatt gtcacagtcc ttttttcgaa
     2521 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     2581 agctattttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc acgacctctt
     2641 ggtataaacg gtgttcaaaa gcttgcatct acgctaatga gtccaaccaa gtcaaaaatg
     2701 aggagctttt ttggctgtcc ttattacttg atgcagggca gttttttcat aatctctttt
     2761 agattaaatc cttttggtta cacaaggttc tcttcaaaga atgttgccat tttttcaaat
     2821 ggaatgatgt ctgtctggtc gtatagatct gtatgggatg ctcctggaat gatcatgagt
     2881 tccttgttgt caccttttag ttctttgaaa gcgtcctccc caaagtaacg tgaatgagct
     2941 ttttccccgt gaatgactaa gactgcatga cggatttctg gaatataatg caatagtttt
     3001 gcattcataa gtgacaaccc tgatgtccta ttccaaccat cattggagtt taaagaacgg
     3061 acatgatatc cgcggtctgt tttataatac gcatgatagt ctttgacaaa ttgaggagca
     3121 tcttcaggca acggatcaac cacacctcca gcacgtgcgt agtccccttg tttgaaatca
     3181 atgatgcgtt gtgcatttaa ttcttcacgc atttcatatc gggcattttc atccataaca
     3241 tcgaagtagc catttgctgt tactcgagtc atgtcataca ttgtggaaac aactgttgct
     3301 ttgacccgcg tatcgatagc tgctgcattc aatgccatac ctccccatcc acaaatacca
     3361 atgattccga ttctctcagg gtttacattt ttttgtacag ataaaaagtc tactgccgct
     3421 tggaaatctt ccgtattgat atcaggcgaa gcaacatatc gaggtactcc tccgctttct
     3481 cctgtaaacg atggatcaaa cgcaatcgtt aaaaagcctc tttcagccat cgtctgcgca
     3541 tatagaccag acgattgttc cttcactgca ccaaatggcc cgctcacagc gattgccggc
     3601 aatttctttt cacttacttc ttttggttca tataaatcag cgactaatgt gataccaaaa
     3661 cgattcggga agctcacctt tcgatgattg acttgattgc tttttggaaa tactttgtcc
     3721 cattcttgtg tgatgtttaa ttctttattc atattctaga ctctcctttg atcaaaattg
     3781 acatttgttt ttgaatgtat tattatgata aaacttaaac ttgactttag gtcaaggtaa
     3841 aaattttatt ttaggaggat ttttttatgt attccattgg acaagtgtct gaaatgtttg
     3901 gattgcctgt ttctacttta cgttattacg acaaagaggg attatttcct gatatccagc
     3961 gtgtttctgg tattagaaaa ttttcagaga atgagttaga ggctttaaga gtcattgact
     4021 gtttgaaaaa atctggttta gaaataaaag acattaagca attcatggaa tggagcaaag
     4081 aaggcagtaa gacatttgag gttcgtaaag aactatttga aaaacaaaaa gaagtagtag
     4141 aaaaagaaat agcaaaatta gaaagagtac tcgctatgct aaactacaaa agctggtact
     4201 atgaagaagc aataaaagcc ggcaatgaag aagccgtatt gacaatgatt cctgatgatt
     4261 taccccaaca tgtaaaagaa gcttatgtac attctcattg aacaaacgga taactcactc
     4321 tccttctcta tgaacttcat gaaaacatga ataaacacct ctaactgatt gttagaagtg
     4381 tttatttttt cactcaagta tcctgcactt acaaaaatat cctttttacc gccatcctaa
     4441 ttctggagcg atatctttta cgattgtttc aagcaagtgc atgttatatt ctacccccaa
     4501 agtattagga atcgtgacaa gtactgtgtc tgcttctttg attgcttcat cttcagctag
     4561 ctccttgatt aattgatcag gttcaccagc aaaagttttt ccaaaaatcg ttgggtttcg
     4621 gtgataagca agatagccga cagcatcttg ttgcggacta ccgtcttgtc caaataagcg
     4681 tcgatccaaa tcagtggtga ttggctgaat cgagcgagta actaatgttc ttggttcaaa
     4741 atcatgacct gcttgcttcc acgcttcttt atacgcacgt aattggtttg cttgttggat
     4801 atggaatggc tcgtgggatt cgtaattgac taatgtagat gattgcagat tcattccatg
     4861 ttgtgctgcc cagattgccg tttgctgcga accagctccc caccagattc tttgtcgtaa
     4921 tccttctgaa taaggttcta tccgtaattt tcctgatcct tgtgggaaca ttggttgagg
     4981 atttggttct tcaaatggtt ttccttcaag taattccaag aattcaaggg tgcgttcccg
     5041 tgttacctct ttgatttctt cttcactgta atcataacca aagtatttcc agccatctag
     5101 aacttgttca ggtgaaccac gccccactcc tagttgcact cttccttgcg tgatgatatc
     5161 tgtcaatccg gcattttctg ccatataata agggttttca tatcgcatat caataagtcc
     5221 tgtaccgatt tcaatcttac ttgtctttgc gccgattgcc gctagcaaag gaaacggcga
     5281 cccaatctga ggggcaaaat gatgtacgcg gaaaaacgct ccgtccactc cgattttttc
     5341 tgcttccacc gctaaatcaa tcgattgcaa ataagcatct tgagctgatt tcacaagcga
     5401 tccttgctcc atccaatgtc caaagcttaa aaagccgatt tttttcaagg taatgacctc
     5461 ctttaaacct acaaaaatgg aaggtttttt atttttaata tgaactgatt ctactacttt
     5521 ttttcttaaa agacgaataa ttgttctcgc ctcacaatca agaaatgtat cggcttacta
     5581 cacccctact ttcttcatca tctgccgtgt cttaatctct tttaacagtc tgttacatcg
     5641 ttgatttttg actcaaaatg attgtttcta acatgtattt atggtaaaaa agacaagatt
     5701 agttctttgt acacatcgat ttatacgctg aacaaaaaga aatatataaa ctaagaaacc
     5761 cattgttttt tcgctccatc acatagagtt tactttagat aaacagtgac atttactaat
     5821 ccataacgga ggaaatcaaa tgaaaaaaat ctatttctta tgtacgggca attcatgccg
     5881 cagtcaaata gcagaaggct acgcgcgtaa atacttgtct ccttcaatgt ttgagataag
     5941 aagtgcgggg atcgagacac at
//
LOCUS       DAJSVF010000127         5921 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSVF010000127 DAJSVF010000000
VERSION     DAJSVF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.134"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            182..619
                     /locus_tag="ON550_002646"
     CDS             182..619
                     /locus_tag="ON550_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955670.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            714..1598
                     /locus_tag="ON550_002647"
     CDS             714..1598
                     /locus_tag="ON550_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3955671.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1822..4989
                     /locus_tag="ON550_002648"
     CDS             1822..4989
                     /locus_tag="ON550_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955672.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            5009..5554
                     /locus_tag="ON550_002649"
     CDS             5009..5554
                     /locus_tag="ON550_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCR3955673.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5582..>5921
                     /locus_tag="ON550_002650"
     CDS             5582..>5921
                     /locus_tag="ON550_002650"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCR3955674.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDI"
BASE COUNT         1995 a         1006 c         1299 g         1621 t
ORIGIN      
        1 ataaaactgt tcatctaaat ctttttatta gaactaaatc aacagtagtt taattactac
       61 ttgatttagt tctttttatt ttaaaaattt tttttactta gaatccagcg accctagata
      121 tttttctgta taatcttaaa taaagaaaac aatatcaaaa aatgcacgta gaagtagata
      181 aatggagtgg ttttgcaaat ttcttttatt tctagaagtt attcacgtat acggatttgt
      241 ttttctcaca aataaaaagg caggaagtac aatggatcat ttgtatataa aatatattca
      301 tcaattttcc gttttggtgt taggcacact aattatagcg gggattgtcg catttattat
      361 tcaaggccgt gacatattaa cagtacatca agagtttttc agttatgcat ttggtattcc
      421 tctgatcgct ataaggtctg ataacaaaaa gtggaagaaa cgctattaca ttctcttgct
      481 atttttgtgt ttttatctgc cacctaagat ttttacttta ttaggtatac aatgcctatc
      541 attttctttt atttcctata tcattgtttt aattttcctc agtttcatag tatggatgtt
      601 ttatcttgtc aaaaaataaa tgaatgtttc atgaacagta cttcatcaca gcctaatttt
      661 tttctttatg ctatcgttat attaaagaaa tcaatcagaa gggaaagaaa gtgatgaaaa
      721 aagttttatt agtggtaaac cccagttcag gcggtgaaca agcaaaggaa tttgagcaat
      781 tagcgatagc aaaattggaa tcggtgtttg atgaagtggt cgtcttgcat acaaaaaaag
      841 caggggatgc aaaaaacttt actcgcgaag cggctacgga agggtatcat agtgtgtttg
      901 tcatgggcgg agatggaaca gtcaacgaag gaatcagtgg aatcgctgaa caagaacatc
      961 ggccaaattt cggatttttc ccattgggta cggtgaatga cttggctcga gcattaggga
     1021 taccgctaga acctgaggaa gcgatcaatc atttttcgat tgaatcagtc aagccattgg
     1081 atattggaaa aataaatgac gactatttta tgaatgtcgt cgcaattggc tctatccctg
     1141 aatcaatcaa tgatgtagat gcggagaaga agacgaaatt tggaaaacta gcttatttca
     1201 tgtcaggaat caaacagtta gcttccacac aatcctactc gtttcacgta gaagttgatg
     1261 gaaagaagga agaaattgag agcagtacct tgctgatagg actaactaac tcagtcggcg
     1321 gttttgaaac attacttcca aatgcaaaag tggatgatgg taagctccat ttggtttatt
     1381 taaaagattc ttctttgatc gatacgctta aaacattgcc agacctatta aaaggagtag
     1441 atgaatctac gaaaaatcta gcctacctta cttgtgagca attagttgtg tcattagctg
     1501 atgaggaaga attgacgacg aatgttgatg gagatgaagg ggataaatta cctgtgacaa
     1561 tcaaagtatt gccctcacat ttacaggttt attgttaaaa tggaggttgt gaccgaggag
     1621 ttggcgggag aacaccgctt gttcgggata agggactgtg acaagacttt tgtcacagcc
     1681 ttttttttgt acaaaaaaga aatttacacc taatagaaaa atttttccat aaaaatacat
     1741 attttaaata tgaaagtaca attggaaata gattaaaacg ataaaataaa attgtcaaaa
     1801 cgtcagagag gatggtggag aatgaatcaa tgtgtaaaaa agctgattgc gtcgattttg
     1861 tttgtcttcc ctctcttgat ggtgacatcc gtttttgccg atgtagagaa aacaaacgaa
     1921 tcaacgcaat cagagaatca aagttttgac aagactactt tttcagaaga atcgattact
     1981 tctgaaacta cagaatctca aagtgaggct tctactaata aaaggcaaga agaaaataag
     2041 acaagtgaga cctcagaagt agctgaacaa acgtctgata gcagtgaagc ggcactggca
     2101 agagctgttt cagcggatgg gtacagcgaa gctgccacaa cagaagaact ggcgcagctt
     2161 ctcgcagacg aatcagtaac taagatccga ctgattcagc ctttgacttt agacagggaa
     2221 ttagagatca aacgagatat cgttattgat tttggtggat tcgctcacaa tttcggcaca
     2281 caccatattt atatcaatga gatacctcag tcgatcgagt tccagaattt taaaggaaca
     2341 gctgcacatc caggtggatt gacaccaaat atcgatggaa atgccattat cattgcttat
     2401 atgaccgatt tttggggaag cagatatcat tttacgggag aaataaaatt taccgggaca
     2461 cttgatttgt atgatgggtc caaattagga ctgatttacg ctcctcgtgc aactgtcaca
     2521 ttagatggtg tttccggtgt gttggatgtc caaccagtga aggaaggact gaatgccgct
     2581 ccgggtaaag cctatttttg tagaagctat caattgaatg tgatcaatgg tagtcaactt
     2641 tatggaccgt atctagggaa attctacggt tttctagatt caggagatga gactggtagt
     2701 ggaaaaacag cgccaggaat ccatatctta agcggttcaa aggtctctct tgattatgat
     2761 cgggcagatg gcgttagcga tgaaggagag gcagtcgata cgctacctgg taatgtcatg
     2821 tttgaagtca gtggttcggg aagcgaattt tcggtaaata caaagatcaa tattaccaat
     2881 gacaacaatc gcggaatcat ccaaatgaga ggttcaggat cgaaagtcac tgtatcgaat
     2941 ggtgggaaaa tcaccatcaa tacagcaact acgggcggat ttcgcttgca aggagattct
     3001 agtcaaatcc atgtatcaag tggtggacaa atctcggtca aaatggctgg agatgacgat
     3061 caacccggga acaatgggat gcgttttgta ggaaaaggcc tttctttgat cgtagaagga
     3121 gccggatcag ttatcgacat cgataagcag agtgggcgtt ccagcgcagt tcgttttgag
     3181 aatggtacgc aaaagctgac agtaaccaat ggaggctcat tgagggtaag aaatacaggt
     3241 gatggcaaat cttacgtgaa cagactcaat caaggaaatc aagcgatcca attttatgac
     3301 gcttctggtt ttctagaaag tccaggttca gctgatttta cagtcactgg agaaaaatca
     3361 aatatcgata tacgcgcaga ttccggagca actgtagata caactggaga tattgattta
     3421 aattttacgg caggtgaaaa aacttattta gtcatggtcg ggaaaacagg ggaagataag
     3481 accggcatca tcagtgggga tgtgttgaat gtggacttag aatctcctac ctattttgat
     3541 ttccaaaaca aacggattgg aagcgagacc aatggcatag gcggctgggt attccaagga
     3601 aatggagatt cttctttaaa gatcgcccat tcagaaatcg ctttatggcg aaaaacaggc
     3661 gcaacattcg ataactttga aaaagacccg gattattttt ctgatatgac agacttatcg
     3721 atcaatggga ccgacttagg caatttcgta tcaagcccta gtgcaggttt aacaaatgaa
     3781 tttgctcaga caggtgccgg aatgaaaaat tataatcgaa tcagtgccaa taatcagctc
     3841 ccgatgattg aatccttaag agtgccaacg aatgcagata aaaaaatcta tgggcatgtc
     3901 gttgttcccg aaggtgtcga ggcaaagcca agagatgctt ggacaggcga agtagaagtg
     3961 actttacgta ttacttacgc agatacgacg aaatcatctg tagaaattac tgctctcacg
     4021 caaggacaaa cagatgaaaa tgaaggggca ggatataacc catggggaga aggcgaacaa
     4081 ggcggcttgt tcgaagttca agtacccaat gatgagagat taaaaacgga ggatcaagtg
     4141 caagttgttg ctgctaaaaa agtaagaggt ggccaagaag tgacagaact ggactctgcc
     4201 aaaacgacag tcgatgtgat tcctcctgaa cctgctaatt taagtaccac attgatcaat
     4261 gcagcttctc gcacgatcac aggtacaggt gaagcaggag ccttggtttc attgaaaaaa
     4321 aatcaagctg ttttgtcaga aacaactatt gatgatacag gggaatttgt tctttctatc
     4381 ccagaaaatc aactttttgc aggagatgta ttagaaattg tattaaacga ccaagcagga
     4441 gaagctactg ctaagggtgt gcagaacaag ccaagtacca acgatgaaat tgggaatcac
     4501 aatccaagtg atacgccagt tgtttatcat gatgccccgc cttttgcacc tgctacgaag
     4561 atcgtagtag aaggtggtct ctcatttctt gccccttcca aactcgattt tggtcagatc
     4621 aaagcaactg gtttgacaca agaagcattt ggccaaatgg atatacagga aaaattatgg
     4681 atttatgatc agcgagaaac gaaaaatccg tggatcttaa cgataaaatt atctcagcct
     4741 ttcacaaatg atgcaggatt ttctatgcag gatgtcctat atttcaagaa aggagaggag
     4801 tatcaattag tgaatgaaga agaactggaa attgtcagag gacaaaattc aacgaatgtg
     4861 caagcagatt attcagaata tttaaataac ggtcgggcgt ttaaacttgt cttaaccaaa
     4921 gaaaatcaga tagctggtaa ttactcagca gagataacat ggacattagc agatgcgccg
     4981 acagaataaa gatcaaggag gatgattgat gaatcgaata tggaataaaa gtcatcgaaa
     5041 caactgctct ttacttatga tccttctcct tggattcatc ctttggcaga cacctgtcca
     5101 tgcctccgaa ttcaattttg cggtagattt tgaattaccg gctaatcaga ttgataagaa
     5161 caaaacatat ataaatgtaa ggatggagcc tgggaaaaca caggaaatcc cctacacggt
     5221 ctttaaccga acagataaat cagtaacagt tgagacattg atccactccg ctacgacgaa
     5281 ttcaaacggt gtgatcgaat atggagaatc aaaaagtaaa tttgatgaaa cattaagtta
     5341 tgatatgaaa aacttagcaa aaacagtggg agaaataact gttcctcctc aaagtgcggt
     5401 cactgagaca ctttcactga aagcgccaga aaaagagctg gctggtgtgg tcgcaggagg
     5461 gatcacgttt caagaaaaaa aagagaacgt ttctaacaaa agtacagttg gtgtcgtcaa
     5521 tcagttttct caagtgaaag caatcttgat ttgaaatagc gaaaaagagt cttccccaga
     5581 tttgaaatta aaaaaagctt atgcggatca aagcgatgga agaaatgtga ttaaggcgaa
     5641 tcttcaaaat ccaaatgctt cttatatgtt cggcttgact acatcggttg aaatctacaa
     5701 aaaagaaaca aaagagcctt acctttcaat aaaaaaagaa aaaatagatg tagcacccaa
     5761 tacaaatttt gatttacttg tcccattaga aggaaaaaaa ttagctccgg gacattatca
     5821 agcggttgtg aagggtgctt ggaaagaaaa acgctggaat ctccataccg attttgaaat
     5881 aactgatgat gcagcggacc aattgaatga aaaggatatt g
//
LOCUS       DAJSVF010000128         5902 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSVF010000128 DAJSVF010000000
VERSION     DAJSVF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.135"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..169
                     /locus_tag="ON550_002651"
     CDS             <1..169
                     /locus_tag="ON550_002651"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3955675.1"
                     /translation="WGTLKVEMFNLDTFDRPAYLDRKIKAYIAFFNNERVTLDMGLAI
                     PTEEKILQMIA"
     gene            complement(254..3178)
                     /locus_tag="ON550_002652"
     CDS             complement(254..3178)
                     /locus_tag="ON550_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR3955676.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3324..3899
                     /locus_tag="ON550_002653"
     CDS             3324..3899
                     /locus_tag="ON550_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955677.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4113..4808
                     /gene="vanR"
                     /locus_tag="ON550_002654"
     CDS             4113..4808
                     /gene="vanR"
                     /locus_tag="ON550_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3955678.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4786..>5902
                     /locus_tag="ON550_002655"
     CDS             4786..>5902
                     /locus_tag="ON550_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3955679.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVEL"
BASE COUNT         1823 a         1119 c         1122 g         1838 t
ORIGIN      
        1 ttggggtacc ctcaaggtgg agatgtttaa cctggatacg tttgacagac cagcgtactt
       61 agaccggaaa atcaaggcat acatcgcgtt cttcaacaat gaacgcgtta ctttggatat
      121 gggattagca attcctacgg aagagaagat tctacaaatg attgcataaa cgctacaaaa
      181 aaagaccaca catttttaca tgcatggtct aaaatacttt gtttttttac ctgtctactt
      241 gacagggggc agttcactct gagttaaaat ggtattctcc tagtaaatta atatgttccc
      301 aacctaaggg cgacatatgg tgtaacaaat cttcattaaa gctacctgtc cgttttttat
      361 attcaactgc tgttgttagg tggagagtat tccaaatact tatagcattg ataattatgt
      421 ttaaagcact ggctctttgc aattgatgct gtatggtgcg ttctctaagc tcaccttgtt
      481 ttccgaagaa aatagctctt gccaatccat tcatggcttc tcctttattc aatcctcttt
      541 gtatttttct tcttaatgat tcatccgata tataattcaa aataaagatc gttttttcta
      601 ttcggcccat ctcacgtaag gctgtagcta agctgttttg tcttgaatag gaacctagct
      661 tccccataat aagggatgct gaaactgttc cctcccttat agaatgagct aatcgcaaaa
      721 catcctcata attttcttta atgacctttg tatttatttg tccacgtaaa atggcttcta
      781 gttttggata ctcacttgct ttatctatcg taaataattt tgagtccgat aaatccctta
      841 ttcttggggc aaatttaaat cctaataaat gagtcagtcc gaatatttgg tcagtgtaac
      901 cggcagtgtc tgtataatgt tcctctatgt ttagatccgt ctcatgatgt aacaaaccat
      961 ccaaaacatg aatcgcatct cttgaattag tatgaataat ctttgtgtag taagaagaga
     1021 attgatcact tgtaaatcgg tagatggtgg ctccttttcc agttccataa tgtggatttg
     1081 catctgcatg tagtgatgaa acacctagct gcattctcat accatctgac gaagatgttg
     1141 taccgtcgcc ccaatagaaa ggcaattgta atttatgatg aaagtttact aatatggctt
     1201 gggctttatt catggcatct tcatacatgc gccattgaga tacattggct agttgcttat
     1261 atgtaagtcc gggtgtggct tcggccatct tgctcaagcc aatattcatt cccattccta
     1321 aaagggcagc catgataatg attgtttctt ccttatctgg ttttcgatta ttggaagcat
     1381 gagtgaattg ctcatgaaat cctgttatat gggccacatc catgagtaaa tcagttaatt
     1441 ttattcttgg tagcatctga taaaggcttg cactaaattt ttttgcttct tctggaacat
     1501 ctttttctaa gcgtgcaagt gatagctttc ctttttcaag agaaacccca tctaacttat
     1561 tggaattggc agctaaccac tttaaccttt cattaaagct gctggttctc tccgttatat
     1621 aatcttcgaa tgataaacta actgataatc tcgtattccc cttcgattga ttccatgtat
     1681 cttccgaaaa caaatattcc tcaaaatccc tatattgtct gctgccaaca atggaaacat
     1741 ctcctgcccg aacatgctcc cgaagttctg ttaaaacagc catttcatag taatgacgat
     1801 taattgttgt accatcatcc tcgtataaat gtcttttcca tcgttttgaa ataaaatcca
     1861 caggtgagtc atcaggcact tttcgctttc cagattcgtt cattcctcgg ataatctcaa
     1921 cagcttgtaa aagtggctca tttgcctttg tagaatgaaa ttccaatact cttaatagcg
     1981 ttggcgtata ttttcttagt gaataaaacc gtttttgcag taagtctaaa taatcatagt
     2041 cggcaggacg tgcaagttcc tgagcctctt ctactgaaga gacaaaggta ttccattcaa
     2101 taaccgattc taaaacctta aaaacgtcta atttttcctc tcttgcttta attaatgctt
     2161 gtccgatgtt cgtaaagtgt ataactttct catttagctt tttaccgttt tgtttctgga
     2221 tttcctcttg agccttacga ccttttgata acaaactaag tatttgccta tcatgaattt
     2281 caaacgcttt atccgttagc tcctgagtaa gttgtaataa atagatggtt aatatcgaat
     2341 aacgtttatt ttcttgaaag tcacggaatg catacggctc gtatcttgag cctaagcgag
     2401 acagctgcaa caggcggtta cggtgcaaat gactaatttg cactgtttct aaatccattc
     2461 ctcgtatgta ttcgagtcgt tctattattt ttagaaaagt ttcgggtgaa ggatgacccg
     2521 gtggctcttt taaccaaccc aatatcgttt tattggattc ggatggatgc tgcgaggtaa
     2581 taatcccttc aagcttttct ttttgctcat ttgttagaga tttactaacc gtattaaata
     2641 gcttcttttc agccattgcc cttgcttccc acaccattct ttcaagtgta gtgatagcag
     2701 gcagtataat tttgtttttt cttagaaaat ctatgcattc atgcagtaga tgaatggcat
     2761 caccattttc caaagctaat tgatgaaggt acttaaatgt cattcgatat tcactcaggg
     2821 taaaagttac aaagtcgtat tcacttcgaa tttctttcaa atgatcccaa agtgtatttt
     2881 ccctttgagg ataatgatca agcgaggatg gactaacacc aatctgtttc gatatatatt
     2941 gtatgaccga atctgggatg cttttgatat gagtgtatgg ccaaccggga taccgaagaa
     3001 cagctaattg aacagcaaat cctaaacggt tttcttccct ccttcgctta ttaactattt
     3061 ctaaatcccg tttggaaaaa gtgaagtagg tccccagtat ccattcatct tcagggattt
     3121 gcataaaagc ctgtctctgt tccggtgtaa gcaattctct acctctcgca attttcattc
     3181 agtatcattc catttctgta ttttcaattt attagttcaa ttatatatca atagagtgta
     3241 ctctattgat acaaatgtag tagactgata aaatcatagt taagagcgtc tcataagact
     3301 tgtctcaaaa atgaggtgat attttgcgga aaatcggtta tattcgtgtc agttcgacta
     3361 accagaatcc ttcaagacaa tttcagcagt tgaacgagat cggaatggat attatatatg
     3421 aagagaaagt ttcaggagca acaaaggatc gcgagcaact tcaaaaagtg ttagacgatt
     3481 tacaggaaga tgacatcatt tatgttacag acttaactcg aatcactcgt agtacacaag
     3541 atctatttga attaatcgat aacatacgag ataaaaaggc aagtttaaaa tcactaaaag
     3601 atacatggct tgatttatca gaagataatc catacagcca attcttaatt actgtaatgg
     3661 ctggtgttaa ccaattagag cgagatctta ttcggatgag acaacgtgaa gggattgaat
     3721 tggctaagaa agaaggaaag tttaaaggtc gattaaagaa gtatcataaa aatcacgcag
     3781 gaatgaatta tgcggtaaag ctatataaag aaggaaatat gactgtaaat caaatttgtg
     3841 aaattactaa tgtatctagg gcttcattat acaggaaatt atcagaagtg aataattagc
     3901 cattctgtat tccgctaatg ggcaatattt ttaaagaaga aaaggaaact ataaaatatt
     3961 aacagcctcc tagcgatgcc gaaaagccct ttgataaaaa aagaatcatc atcttaagaa
     4021 attcttagtc atttattatg taaatgctta taaattcggc cctataatct gataaattat
     4081 taagggcaaa cttatgtgaa agggtgataa ctatgagcga taaaatactt attgtggatg
     4141 atgaacatga aattgccgat ttggttgaat tatacttaaa aaacgagaat tatacggttt
     4201 tcaaatacta taccgccaaa gaagcattgg aatgtataga caagtctgag attgaccttg
     4261 ccatattgga catcatgctt cccggcacaa gcggccttac tatctgtcaa aaaataaggg
     4321 acaagcacac ctatccgatt atcatgctga ccgggaaaga tacagaggta gataaaatta
     4381 cagggttaac aatcggcgcg gatgattata taacgaagcc ctttcgccca ctggagttaa
     4441 ttgctcgggt aaaggcccag ttgcgccgat acaaaaaatt cagtggagta aaggagcaga
     4501 acgaaaatgt tatcgtccac tccggccttg tcattaatgt taacacccat gagtgttatc
     4561 tgaacgagaa gcagttatcc cttactccca ccgagttttc aatactgcga atcctctgtg
     4621 aaaacaaggg gaatgtggtt agctccgagc tgctatttca tgagatatgg ggcgacgaat
     4681 atttcagcaa gagcaacaac accatcaccg tgcatatccg gcatttgcgc gaaaaaatga
     4741 acgacaccat tgataatccg aaatatataa aaacggtatg gggggttggt tataaaattg
     4801 aaaaataaaa aaaacgacta ttccaaacta gaacgaaaac tttacatgta tatcgttgca
     4861 attgttgtgg tagcaattgt attcgtgttg tatattcgtt caatgatccg agggaaactt
     4921 ggggattgga tcttaagtat tttggaaaac aaatatgact taaatcacct ggacgcgatg
     4981 aaattatatc aatattccat acggaacaat atagatatct ttatttatgt ggcgattgtc
     5041 attagtattc ttattctatg tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag
     5101 ataaataccg gcattgatgt acttattcag aacgaagata aacaaattga gctttctgcg
     5161 gaaatggatg ttatggaaca aaagctcaac acattaaaac ggactctgga aaagcgagag
     5221 caggatgcaa agctggccga acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat
     5281 attaaaacgc cccttacatc cattatcggt tatttgagcc tgcttgacga ggctccagac
     5341 atgccggtag atcaaaaggc aaagtatgtg catatcacgt tggacaaagc gtatcgactc
     5401 gaacagctaa tcgacgagtt ttttgagatt acacggtata acctacaaac gataacgcta
     5461 acaaaaacgc acatagacct atactatatg ctggtgcaga tgaccgatga attttatcct
     5521 cagctttccg cacatggaaa acaggcggtt attcacgccc ccgaggatct gaccgtgtcc
     5581 ggcgaccctg ataaactcgc gagagtcttt aacaacattt tgaaaaacgc cgctgcatac
     5641 agtgaggata acagcatcat tgacattacc gcgggcctct ccggggatgt ggtgtcaatc
     5701 gaattcaaga acactggaag catcccaaaa gataagctag ctgccatatt tgaaaagttc
     5761 tataggctgg acaatgctcg ttcttccgat acgggtggcg cgggacttgg attggcgatt
     5821 gcaaaagaaa ttattgttca gcatggaggg cagatttacg cggaaagcaa tgataactat
     5881 acgacgttta gggtagagct tc
//
LOCUS       DAJSVF010000129         5877 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSVF010000129 DAJSVF010000000
VERSION     DAJSVF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.136"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(505..1314)
                     /locus_tag="ON550_002656"
     CDS             complement(505..1314)
                     /locus_tag="ON550_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3955680.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1314..2060)
                     /locus_tag="ON550_002657"
     CDS             complement(1314..2060)
                     /locus_tag="ON550_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3955681.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2078..2545)
                     /locus_tag="ON550_002658"
     CDS             complement(2078..2545)
                     /locus_tag="ON550_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3955682.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2545..2955)
                     /locus_tag="ON550_002659"
     CDS             complement(2545..2955)
                     /locus_tag="ON550_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3955683.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2966..3979)
                     /locus_tag="ON550_002660"
     CDS             complement(2966..3979)
                     /locus_tag="ON550_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3955684.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4196..4930
                     /locus_tag="ON550_002661"
     CDS             4196..4930
                     /locus_tag="ON550_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3955685.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVRNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4914..5612
                     /locus_tag="ON550_002662"
     CDS             4914..5612
                     /locus_tag="ON550_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3955686.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSHS"
BASE COUNT         1902 a         1232 c          978 g         1765 t
ORIGIN      
        1 cgcaaaacat cagcttaaag cgacaaaaac cacgagcagg tccattcatc ctcaaacctc
       61 ggtgatcttc gcggtttaac accatcagaa gaaatgcaca aaaataataa ggagagtctg
      121 agacataaat aaattcccag acttttagtg agtctaaatc cgaataaacg gtgggtcgaa
      181 agcaactcta gctgcgcgtc gatcgcagca atctctaggt gctatgttcg gataaatggt
      241 ggtcaaaagt cagattttcg gccatttcgt aacagccaac cagacaaaaa gcgtctgttt
      301 tgtcttctac tccaattgct caaatcttag cgacttttgg cttggtctta ctctaaacag
      361 tttgtaagcc taaactttat ttgctgtttg tttctaagat caaagcaaaa ctacttttta
      421 gttggttact tttacaagtt ctttttactg ttgaataaat gtaaatctta agaacaaata
      481 tttacttcta taattccaag tttcttaaaa aattccgcca agtgtaccta aaaagcataa
      541 aacaaaaatt cctagaataa ttgtgatcgt tttatgccct ttggagtaaa gccagacgca
      601 taaaaaggta attcctaatg gcagcaagcc cggaaaaatt tgatcaaaaa tactttgtaa
      661 tgaagtttca gctccaccaa ccgtgaattt tagtgacgta tttaaagtaa ccatcgtgcc
      721 tatcatcgcg ccaattaccg ttaaacctaa aatcgaagca ccatgagata atttgtcaat
      781 catatggttg tcttctgtat catcaataaa tttagttcct aaaacatagc catattttgt
      841 cgccaaccat ttaaaaataa aatgaatggc agtatacaaa gtaaaataaa ggatcatgcc
      901 taaaatattc ccttgttgag caaaagaaat acctatgcca gccgcaataa cccgaaaagt
      961 tccccagaaa aatgaatcgc caatacctga taaaggtccc attaatcctg ttttgatttt
     1021 attaattgcg tctgtatcaa attcaggatt tttagcattt tcttcttcca tggtgacgga
     1081 caagctcata acaaaaggcg caatatgcgg tgtaatgtta aatgtttccg tatggcgctg
     1141 caacgctgta taataatcat catcgttcgg atatatttta cgtaaggaac gttcaatacc
     1201 attcatttgt ccatatgcca tcattttatc gaaggaccaa gaggattgca taaaaaagga
     1261 gcgccaaaat atacttctta attctttttt cccaataaca ctttctttta ccattataaa
     1321 tcttcctcct cttcaaaagt tagcttttga acagccagta acgctaagca aagtccgacg
     1381 acagccacac cgacaacatc cattttcaga tacattacgc cgacaaatcc taagaaaaga
     1441 taaggcatca tcgctttatt cgccatcatt ttaattaata acgctagccc aacacaagaa
     1501 agcacaccgg ccgcaacatc aaagctgtcc aatacaacct gaggaatcaa cttgatcatc
     1561 gcattgattg tagacacacc aaaataagta gctacaaaaa ctaaaatggc cgatggaacc
     1621 ccaacgatta aaggttgagc cacataatga tatttttgtg cttttttgaa atcatgattg
     1681 actaatgcct catccatttt gttgttaaaa tacgtaattg gcattgcata ggcgaagtta
     1741 cgccacatct gaagaaagat ggacaaaggt aaagtcaacg caacggcagt tcctactcct
     1801 tgacccgatt gaatcgcaat tgctgtacct aaaacactgg aagcataaac ttcagggggc
     1861 gtcgcactcc ctaccataat tgagcccatg aacatggatt ccaaggtggc accaataata
     1921 attcctgact ctaaatctcc caaaatcaaa ccgacaaaag gcgctgtaaa tagcggtcta
     1981 cccatcatcg atgcaccaaa tgtgtattca tcaatagacg ccaaaaaagc taccagtgcg
     2041 attaatattg cttgtatcat attcttcccc tcctctttta gtcatgcaaa ggattgattt
     2101 ttttgtccgt tggtacagcc tgacattgaa ctgttatccc gttttcagtc atttttttca
     2161 ccaagtccag ttcttgttca ttcaagtaaa cggaactagt aatcattttc cgattctcgt
     2221 ttttctttaa atttcctaaa ttcacttctt taatctcagg tacgtgctga gtaatttcgt
     2281 aggcatcctc caccgtcccg acaattaaaa atacttggta tttcttgatt ttttctccat
     2341 ttaaaacaga gatggcctct gtcatatcct tcaccacaaa ttttgtccct gtaggaacag
     2401 ccatttttag tgtttttttg cgcagattat tggtagcgat ttcattactt gcaacgaaaa
     2461 tacaatttgc acctaagtta tttgtccagg caaaagcaac ttgcccatga acaaaacgtt
     2521 catctactcg taataattta atcattaaaa atcatcctct tctgttttct ctagcaactc
     2581 gttcaccaaa actatccctt cattcccgct attcatagct ttttggatta gttttttcgt
     2641 gtcctgttca cctgataagc aaagctctaa aagcagcggc agatttacac cgctgattaa
     2701 ataaaaattg tctttaccga tttgttcgga gaaagcgtta gacacgcttc ctcccagtaa
     2761 atctaccgtc acaattaatt cgtcatcagg cgctaagtcg gaaaatagct gctctatttc
     2821 ctggttaatg gagtagtcac tatctatata cgcagttatt gctaaaagtg atttttgagg
     2881 accaataata aattctaaag tatcctttaa gcctttagcc atcatggaat gcgtcgcaat
     2941 tacaattttc ctcatagctc ttaccttaat attccacagt ccgataataa cgacgaatat
     3001 ctagtgaatg atcccgcacc ttttcaaaat gagcactcac tcgttctaag gccgttgcca
     3061 taaccattgg cgctacatac tgacgcatag cgcttggtac acctgctaat tcgaaatccg
     3121 cagtgtcaaa aaccgtcaca acatcgctat atttatttac aaaacgctct acgcgatcga
     3181 ctagcggacg cgtttgatct tcacctttta ataacacaaa actcatagat ttatcagtca
     3241 tttcaatagt cccatggaaa aattcggctg catggatcga cttggtggca atccattgca
     3301 tttcttccag tacgcacata gcatattcat aggcagcccc ataagtattc ccagctccaa
     3361 caaacatatg aaattccgta tttttatgtt tttctgcaaa ttcaagtgct ttctgatcat
     3421 tttgttgccg aacacttagt aatacttccg gcattttttg taatgtttga ataaattcat
     3481 catattcagg aaactcaccg ttatttttca tcaggcgtgc acccaaagca aaaaactgta
     3541 actgaagttc ttctactaat gtatcgttgt cagcatcatt ttcaatcgca taatccacga
     3601 tttccccgag tggtgtagca gcatttccta caaacgcaat cgtcgttgca ccttgactat
     3661 tggcatactt aacaacatca acggtttctt gagtcgtacc ggttaatgaa gatgtgatta
     3721 acactgtatc tttattaatc tttttcggct gtgaacaaac cacctctgcg gctatttcat
     3781 gataataatc aagctctgaa tgagtgctta acagataagc tagcgggctg attgtggcat
     3841 atgttccacc agagcctacc aaataaagat ttttaaatcc cttctcactg attgcatctg
     3901 ccacctgatt gattgtctcg atatttttta ctgcaccatt taatactgat agaaaacgtt
     3961 cttcgctaaa acctaacata gttaaaaacc tccaagtcaa taatttgaaa aatatatttg
     4021 caaatgccgg cacattaaca aaccacttaa tcttttttat ataccacctg ttaacgtttt
     4081 taatgtacca cttatagaaa acgctgtcaa tagtttttaa ccacttttat ttttatttgt
     4141 accaaaatcc ttttttcatg tataataaac aaaaatagac tctggagtgt aacgtatgaa
     4201 tactgaagaa ctaacgaaca aattaataca agacattgaa caagaaaaat ataaatacgc
     4261 gcaagctatt ccttcagaac gtgattttgt tcaaatttat gaaatgaatc gttcagccgt
     4321 aaaaaaagcc attcaacaat tagtaaataa agagtaccta ctaaaaatac atggcaaagg
     4381 aacttttgtt aaaaaaaata tccatgaaca tttgcaaatc aaatttcatg gtttgtctga
     4441 attgttggaa cgggaaggga ttcagcctag cagcttaatt ttagcttcag aaataaaacc
     4501 ggctggacac attgtcggga aagctttaca gctggaacca actgaagaag tatttcattt
     4561 ggttcgttta agaagcggcg acggtgagcc aatttctatt gaagacactt atattccatt
     4621 aaatctcata cctgatcttc aagcttttga tttccaaatt ttttcatttt attccacatt
     4681 ggctgcttat cattatcaaa ttgaaaatat ctatcagaca atttccagct caaaagtgcg
     4741 caataaagaa gcccgtttgt taaaaaccag cttaaatcgt ccggtaatca gtcttgctat
     4801 cactgcggtt acgacaaatg aacaagcggt tgaatacact gaagtagtgg ttttgcctga
     4861 attcgccgcc atctatacta aaaatgttca tcaaggaaag gagatgaaaa tctatgccca
     4921 aattgattaa tctgttggct tatgacctga ttgacaaaat tattgcttat attcatgaac
     4981 atcatttaac ccccggaaca aaactgcctt cggaacggga cctatcagaa atgtttaatg
     5041 ttaatcgaac taccttaaga ggcgcgttac aaaaactggt ggacaacggc accttacggg
     5101 cgcaaaccaa tagcggctat tatttaactg cgccaaaatt cgaaaaaaat gcccatcatt
     5161 tctttacacc gatagaagat tctctgctta gtaagcagcg gctacagcta aaggcaacgg
     5221 ctctttcacc gaaaaaagca gcagctcttt gtccggaact gaatgatttc aaacagcagc
     5281 aaccacaagc ttatttagaa tatattgatg cagaagcaat tagcttgatg ttattaatcc
     5341 ctatggaaac attaaagaaa agtaatttct cggacaatcc tttccaagat tttaaaagtt
     5401 tggcatatac gcgttctcaa gaaatttctt taataactga caatgacgtt gcggcggact
     5461 tgctgggagt tgacaaagaa gaggttctgt tatttgtacg agaggaaata agagccggta
     5521 aggagttggc ggcgattgct ttaggttata ctattagtcg ccgctgcaac atacaggtta
     5581 atgttgctac gaccttgcaa tcccatagtt aggaaaaact attcgctgta agttcaaccg
     5641 tttcaatgca agcgaatcac aaatcaaaaa gtatttaact cgtgtttatt agaaatagtc
     5701 atgaactttt agtctccaag atcaaaccga cagcgttcaa ctctgagaag caaaagtaaa
     5761 tgtacgatca agtatgaggc tgggacagaa gcgctaagcg gttctgttgc aaagttttaa
     5821 atctactatc agataaggta gaataataga aaaagatagc aggaggaatg acgatga
//
LOCUS       DAJSVF010000130         5855 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSVF010000130 DAJSVF010000000
VERSION     DAJSVF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.137"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(934..1107)
                     /locus_tag="ON550_002663"
     CDS             complement(934..1107)
                     /locus_tag="ON550_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955687.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1110..1508)
                     /locus_tag="ON550_002664"
     CDS             complement(1110..1508)
                     /locus_tag="ON550_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955688.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1724..2779
                     /locus_tag="ON550_002665"
     CDS             1724..2779
                     /locus_tag="ON550_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HCR3955689.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2799..3152
                     /locus_tag="ON550_002666"
     CDS             2799..3152
                     /locus_tag="ON550_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955690.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3334..3630)
                     /locus_tag="ON550_002667"
     CDS             complement(3334..3630)
                     /locus_tag="ON550_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955691.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3634..4452)
                     /locus_tag="ON550_002668"
     CDS             complement(3634..4452)
                     /locus_tag="ON550_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3955692.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4678..4899
                     /locus_tag="ON550_002669"
     CDS             4678..4899
                     /locus_tag="ON550_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR3955693.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4901..5167
                     /locus_tag="ON550_002670"
     CDS             4901..5167
                     /locus_tag="ON550_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3955694.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5280..5819
                     /locus_tag="ON550_002671"
     CDS             5280..5819
                     /locus_tag="ON550_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955695.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT         2058 a          972 c          869 g         1956 t
ORIGIN      
        1 cgagtgtaaa atttctttat tcctcccacg ctccatgtta ttttatattt cctttttcat
       61 gttattttta tccagaaacc gatcaagttt ttacctattc ccaaaatcca tttattcgtt
      121 atttttttca ctgattaaga ataattattt gatgcttaaa ttagctaaaa aagcgatttg
      181 ttactgatat aatcatgata ttgcagctga attaggcgga aagataaaat gttcgcaaaa
      241 tcaaagtatc aattatcttg tttttttata aatttgtaag taaaaatcta aaaaagtttt
      301 cttttaattg gtatatgtat taaaatcaca tttaatcacc cataaagtat gcgctttcat
      361 ttttttggat gagattaata tcaataaata acgatgtaaa aatcatttat tatgctcttt
      421 tataaggata ttaattgatc taatttgaaa aaaatcaaaa tttaaatgtt ttaatattac
      481 aaataaaatc atgttattgt aaaatggtat aaaaaagcta aatattctat ttttgttatt
      541 ttattagcag gaaaatttaa atagttttac aagaatccgt agaaatatat tggttttgcc
      601 ttcagattaa tactggatca tattaatctg aatataatat atgaactaat aaatagctta
      661 acctcgctat tttgtttttt ctgatcacta tctatacagc attagtaaat agaattttaa
      721 aaataaaaaa attaagacaa aaaagtatca acataaacat atacatatac acgtttatgt
      781 tgattcactt aatttgtcct ttaaaaagca cttattttaa tataatgtta ttttataatc
      841 ttattacaag ctgattgtta ggcgttgtcc tttcattaca tttattaacg gttattttat
      901 tgtttaattt attaattatt tttgatctaa aacttagctt aacaaataaa taattaacca
      961 accgccagat aaaaatgcga tcattgctaa tacataagca aagataaaaa tatgttcgat
     1021 cagcttaaac cctgatgcga ttaagcagac caagtaaaat agaaattctc ttacagataa
     1081 tggtaatttt ctaactagca tactcataat taacacccca aagtaatatt gtattttgaa
     1141 cattttacat ccaatacagc atcaatcaac cattgccaat caaaaataca aggcttcatt
     1201 gaagtatgtt tttcagatga aaagatataa tataatttct tcggaataat attttcagtt
     1261 gttgggatca agctaaatct aataccgtct aactcatagc taccagcttt ctctccatta
     1321 aaaatttcct tagcactttc ttctaaagaa tcaaaatatt tttcgacagc aatctttgaa
     1381 gcttttacta ttgaagcatt tcccttttcg ataaaatctg cttttgtttt accgacaata
     1441 agtaattctt gatcagtaaa atcttctaaa ataatggtag tgtaattctt atttcttctc
     1501 aatcgcatca ggatcactcc ttaatataat ttatgttttt ataatatcat aataagataa
     1561 atttgcaagc atttatttac acctttttat ttacacttta ttataattaa ttagactttt
     1621 atgattgcat ttttttctta taaacagttg caactaatag taaataggtg taaactattt
     1681 tcgtaccgaa ataccataaa cacaaacaga aagaggtcat accatgaaag aaccattatt
     1741 gttttccgtt gacttaggga ataaatcagt aaaagaaatg agtacacacg tttctccact
     1801 ctcacttccc tctcgtattt tgaacaaaaa tatcattaat tcaggacgtt tttctaatat
     1861 aggttcctca ttaaaacgat cactaaacgt ttctgaatat agcctagtaa acactactga
     1921 aagctattac tttgggaagg atatacacga gctaggaaaa gatgactacg tgatagaatc
     1981 actaggtttt gggatcagca gatacaagca ggaaacctac caaaatatcc tatctttctc
     2041 tttagctaac ctagcacgtt tagaagatca atctgatgac gttttaaccg ctaatctagt
     2101 tttaggtctg ccttcatccg actataccga aacaatcgtc aaatacgtga tgcaacaagc
     2161 cacacgacaa cattcagttg aagttgacgg taaagcttat aatataaacg tagataccgt
     2221 ttgggtactg ccacagccga ttggaacact atataacctc ctacttgctg atgatggtag
     2281 tgtacaagat gaaacattga tcagtcagaa gatcggtttt gttgatattg gtggtggcac
     2341 gaaacttttt gatgttgtcc aaaattttga aattgacgaa aacaaccgtg aacaaaagga
     2401 taccggtatt tttactttat ataatcgtat cgtaaatatg cttgaccttc cgatcagtcc
     2461 taatgctttc caacttgaaa agattatccg agaaggcaaa gaaaaaggcg tatatctatt
     2521 taaaccaagc ggaaatgaaa gttttgatat ttcagaagta gttgaaagag aaataaaaac
     2581 atacaccaat atggttgttg cagaagtttt agctacattc aaaaacttca caaccttaga
     2641 taaagttgtc tttactggtg gtggggcttc tattatcgac aaacaacttg taaaagataa
     2701 aattccgcat accttgtttg ttgaagacag tgaattttct aatgtaaaag gcttttacaa
     2761 gtttggttta actcaataaa acagcgaagg agatcaacat gaataaggga aaatcagagc
     2821 gttatgtagt tataatcaat agagaaaagg ttagcgagtt acctttagca gagtttcttg
     2881 aatctgaata tgaaacacta ggcgttaaat actctcagac gatcaaaaaa gctttgaatc
     2941 tatatcggcg tgtaaagaac aatgaagtta caattattga agcaggcaac gatagaaacg
     3001 atagcaaagt agctgaagta aagaataaac caactgatca taaccctatt caacaagcgc
     3061 atccttcaac ggttacatcc gatcattcag caaagaaaga aaagccaatg ctaaaagggt
     3121 tcaatgcacc aagtaacaaa gacttacttt aaaaataaaa aggcgaggtt acaataccta
     3181 gcctttttat tatgtacaaa tacaaacata aacatatacg tatacatgta tataaacatg
     3241 tatacgtata tatccataaa cacgtatacg aaatttagtt aagctatacc taaccgctta
     3301 ttccaataaa aaaagtgatc agctttaaac tgatcactca ccaaaaactt catcatacaa
     3361 gttatctatc atttcaccaa gtaaagctga atctgatcga attcctaact tttcgctgat
     3421 tttagatagt ttctttcttt gtgatggttg aagcgtgaat tgataatttt tcttcttttc
     3481 agaactatca ctttttagtt tagctaccaa agaacttgtt gtttctttag gtgtttgttt
     3541 aggttcttca actgcttcaa ccacttcagg aacttcttct acttcagctt cagcgaattt
     3601 tttaaatcta ttttctttca cttcaatcac tccttattct tgtgccgtat caattatttt
     3661 gttgtatagc gtaaacgtgc tttcaaagaa atccttattc ttacgataga tattcttatc
     3721 cttagcgaac tcagcaatat tcttaccttc taagctagtt gcattaagca attccttctc
     3781 ttgtacatat ccaagataat tagataacgt tggtaccaat tctaggaact cacgacttga
     3841 attagtatta tgtttgatcc tgctaccgat cgtagcgtat ttagtttcca tcagtgtttc
     3901 accagtctta atatcaatca actgttttgc tagtgcatca atatctttac caagcttaag
     3961 caaagcttca aaaccatctt tagatggatc agaaatgcct acgacaagat cagatacagc
     4021 ccaagcattt tgagctagta gacctgcatc attatgagta tcaatcagaa ttagatcata
     4081 cttctcttta agctgttcat agttaacaac gatccatcta aacagtaggg ttctgttgtt
     4141 aggcatatca cgcatttgca tctcaacttg ttttaaatct tcacctgcta ctaaaatatc
     4201 catgttctca tttatagcta aaggttcagc tgatccatga agatataaat gattgattgt
     4261 attctctctt ttcgctttac tgaaagcatc aggaaatcgt tttgataggt tttgcgactt
     4321 atcttcatca atatacagca ccttcatacc taaattttcg acagcatatc cacctagatt
     4381 gtagattgtt gttgttttac ctgttccacc tttttcaatg tttccagtaa tgattgacaa
     4441 cttcttttcc atgattaaac ctcccttatt aatcttttgt aactccatta tacactataa
     4501 acacgtttat gtatatattt atatacataa acgtgtttat atttatattt gcttttatat
     4561 tctacaacat aaacatatat atgtatatgt ttatgtttat gtatatattc aaatatataa
     4621 ctattgcatt tgtcatacaa tcgtagtaca atgtaattga aaaggagttg ataaactatg
     4681 ccagtaataa gtattcgtgt atctaaagaa gaaaaagatt ttcttgaaaa agttgctgaa
     4741 ttcaacggag ataacatttc tgaatttatg cgtagtaatt ccctgcaaac agcagaaacg
     4801 ttagtagact ttgccaccta caagcaacta atgaaagagc atgaggaaaa cgatcaaagt
     4861 atttcccacg aagatatgct aaaagagtta ggcttataag atgacttacc aagtaagata
     4921 tgaacgtgaa gctcaaaaag cgctaaaaaa aatggataag tttcaggcaa aggttatcct
     4981 aaactggatc gagaaaaatc ttgtaggcac tgatgatcca agaaagcatg gtaaaggtct
     5041 aacagctaat aaatcaggct actggcgata tagggtaggt gcataccgta taatagctga
     5101 tattagcgag catgaagtga caattcttat attgtctatt ggtcacagaa gtgaagttta
     5161 taagtaacag acaagctgat aaggcttgtc tgtttttttg ttaacccaac aaaatcactg
     5221 tttacgatcc cactttacct aaaaaagaac aatatcctct tgatattata cacaccagtg
     5281 tgtataatat aagtgtaaac aagctaatta actttcaaaa ggagcgattt tttatgggat
     5341 ggtatggtga ttattcatca gcaaatgaag tgatcgaaga aaagaaaggg tacgccaaaa
     5401 atggtttgga aatcgtaggt accaaagtaa cgaagaccta tggaattatc cttttcaaag
     5461 atgcagaagg aacctacgat attgacagct tcatctttcg agatgaaatg tacaaaccaa
     5521 tctacttctt aaatgacagt cctgcttact ttaagaaaat tccgaaaaaa tggttgaaat
     5581 tctttgtcgg tgatcaagaa aaaatggtta agctacaact agatagaatt aaagaagaaa
     5641 gcaataaacc aaaacttgat gacttattag ttgcaggtaa aacttactca atctgggatg
     5701 gatcgtatgt cgctgtctat cagtataaac tcaaacgtag ctttattttt cgactacaag
     5761 atggatcact cactcggttc actcgactat cagcaggaga tgttaaggaa gtaacctaaa
     5821 acttaaatac ataaacataa acgtgtatac gttta
//
LOCUS       DAJSVF010000131         5790 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSVF010000131 DAJSVF010000000
VERSION     DAJSVF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.138"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(146..544)
                     /locus_tag="ON550_002672"
     CDS             complement(146..544)
                     /locus_tag="ON550_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR3955696.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(541..1314)
                     /locus_tag="ON550_002673"
     CDS             complement(541..1314)
                     /locus_tag="ON550_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3955697.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMVKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1311..1994)
                     /locus_tag="ON550_002674"
     CDS             complement(1311..1994)
                     /locus_tag="ON550_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3955698.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2161..3048)
                     /locus_tag="ON550_002675"
     CDS             complement(2161..3048)
                     /locus_tag="ON550_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3955699.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAAITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3045..3914)
                     /locus_tag="ON550_002676"
     CDS             complement(3045..3914)
                     /locus_tag="ON550_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3955700.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4051..5337)
                     /locus_tag="ON550_002677"
     CDS             complement(4051..5337)
                     /locus_tag="ON550_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3955701.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTGWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5610..>5790
                     /locus_tag="ON550_002678"
     CDS             5610..>5790
                     /locus_tag="ON550_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3955702.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMP"
BASE COUNT         1797 a         1270 c          967 g         1756 t
ORIGIN      
        1 tttgaaaaga aagtctgatg cttagagtag aatttcataa aaatttacta tttcataagt
       61 attcacgaga aaaatacaaa caaaaaagcg gaaataacgc tcatccgcct tcttgtttgt
      121 gtttatttcc tcttcaaacg cttgtctatg attcagactt ttctctatcg ttattttcat
      181 ctaaaagttc ttgcattccg ataatttctc gatattgatg aatcttagtg ataccaatga
      241 ctttgtctac atcaatacta aaacaaatac cattacctgg aatatcgatg ttgatgcctt
      301 gaccaatcgc atccattact ttattcgtca aactatcggg tactaatgtc aacacaatat
      361 ctttttctgg cgaaatgtcc aaacctaaaa atttccctga atcatgtaca ccagaacctc
      421 ttccatgtaa gatcgttccg cctgctgcac ctgcttcttt tgaaaagtcg ataacatctt
      481 cgcccattcc tccgtcaacg atcgtcacaa tcatgtgaag attcaatgct acaagatctt
      541 tcatttgctt tcccccaatt tttgcaatct ttctttttcg attctgtctt tttcatcttt
      601 tcgtttataa atatatccta gtactaaagt ggataatatt ggtgtcaatg caatcattgc
      661 aaccacacca aacccgtcaa tgatcggatc tcttccttca atttgtgcag cgattgccac
      721 gctcattgat aaaataaatg tcgaacacat cgggcccgtt gctactccac cgttatcaaa
      781 agccattgct aaaaagatct tgtccacttt tcgtcctaaa ataaatgcta gcccgtatcc
      841 tgggatcaag aaataataaa gagagaatcc gacgagcaaa cgccacatag acaatcctac
      901 tgctgcgccg atcccaattg aaattaccat cagcatgatt ttcgatcgga ttctcccctc
      961 gctcaattct tcaacctgtt tgaccattac atgaatcgcc ggctcagcaa accctactaa
     1021 gaaccccatt aaaaagccaa ggggaaccag tatataattg taaggcaatc ccgcaatagc
     1081 actgcctaaa tgctgtccta ccggtacata agccatattg actccgtgca ggaaaaggat
     1141 caacccaata gtcgttatca agaaaccttt cataatgtca aagaaacgct ttttgcgcat
     1201 acgaaaacta atgatattca ttaaaacaaa taggattacg ataggtgcca ctgccaaaat
     1261 gacttccatg gtaacgattt cgattccatc tatcacttgc tgccagaaac tcattggaag
     1321 atcaccccca aaatcatcac ggcaagaatc ggccctaacg aagcaatacc gaccattcca
     1381 aaactgtcat tttcattttt cttttgtttg atcatactag tcatccctcc ggcaagggcc
     1441 aagataaatg gaacagttac tggaccagta gtcactcccc ccgaatcaaa agcaatcggc
     1501 ataaattcgg aactggtgaa aaatgaggcg atcaaaacgc ctatgtagcc tatagccatc
     1561 aattgataat aggatagctt gaatacgacc cgaagcagcg caaaggctag aaatactcca
     1621 gtgccgacgc tgacgatccc aatcagcaac actctgccaa tctctccgtc agatatatca
     1681 tatacttgtt gtcccaaaac ttgcacggaa ggttcagcaa tcgtgatcac gatcccgata
     1741 gcaaatccca aactgaccaa aacagtcaaa cttttctttt tgatcatata tttccctact
     1801 agatctccga cttccatcat agaataatct gctccaaaca aaaagagcgt cattccgatc
     1861 atcatgattg ccgcaccaac cagaaatgaa gcgaggtctt ctccttctaa cggtgcaaaa
     1921 acaaacgtta aaatgacaat caggacagtc atcggcaata tagcagcaat cacttcttta
     1981 aaatttttta gcatgtatcc tcctccttta ctctttaagt ttaccttaaa atgaaagagt
     2041 tttcgagcac aaacttaaaa cgttatcatt taatttttct caacataaaa taacacacgt
     2101 cccaaaagta tctttttact tggaaaaatg actttgaagg aacgcgctta ttgtgttttt
     2161 ttattttaca gagccgatca ttccttgaac gaattgcttt tgcaaaacaa aaaatacgat
     2221 ggcagttggt aaagtagcga tgactaatcc agccatgatc attccatagt ctggtgtata
     2281 ggaagcaccc atagcagata aaacaagcgg aaccgtccgt ttatcaggag attgcaaagc
     2341 tactaagggc cataaataat tgttccagct actcatgaat gtgatggctg cagccgcggc
     2401 ataggtattt ttagccgtcg gcatatacac tctaaaaaaa atacttagtt cttttagacc
     2461 gtctaatcga gcggcttcta ctagatcttt tggaaaagat ttaacatttt gtcgaaagaa
     2521 gaagatcaaa aaagcagtgc taacagatgg caggatgaca accaagaaac tgttgattcc
     2581 caaaaccgtt ccgttcaatt gtgaaaacat tcgatataaa gggatcatca atgccgcaaa
     2641 aggaaccatc atcgatagca gtagaatatt gaaaattagg tcttttcgtt tactttgata
     2701 aatctcaaat ccatatcctg ccattgacga aatcagcaat gcaaacacag tggtcaccaa
     2761 agcgatcatc gctgaattcc aaagggaacg agtaaaattc agctcattgg aaaacaaatt
     2821 ttgaaaattg atgatcaagt tactgccaat ctttattttt cccgatgtga tatccacggg
     2881 tgtattcgtc attcccaaaa ccatccagac aaaagggaaa atggaaataa atgataggat
     2941 gcttaatgct gtatacttta atactgaccc aattgtcgaa cgaagttcta atgcttgttt
     3001 tttgacgttg gggcgttcga ttgtttcttt ttctaccata tttttcatcg actttctcct
     3061 cctactttcg tttggattat cgacaatatc acaataaagg aaacaataac aaaggaaact
     3121 gctgccgcat atccaaaatt aggcgtgaat ttataactca aattatagat gtattgagat
     3181 agggtggtcg ttgcatttgc tggtcccccg ccagtaatat tttgtacttc atcaaaaagc
     3241 tgcaatgtcc cgatcgtcga agtaatggaa gtaaacaaaa tgattggttt taaatttggg
     3301 atcgtcactt tgataaactg ctgccattta gaagcaccgt caatagatgc tgcttcataa
     3361 atctccgaat caatattttg catgcctgaa aggaaaaaaa tcatattgta tccagtccaa
     3421 cgccaagtga tcgaaagaat gatcagaact ctcgcccaaa ttggatgagt caaccagcca
     3481 atcggtgtgt caatcatacc aatgcccaac aacaagttgt ttacaaggcc agattgagaa
     3541 aatagacttt tcatgatcag agaataactg accatcgaag tgatacatgg aagaaagata
     3601 gcggttcgaa acaacccttt atatttcaac tttttatcat ttaaaaggtt ggaaataaaa
     3661 agtgctagaa acaacatgat cggtacttgt acaagtaaat acaggaatgt attaaaaaaa
     3721 gcttttttaa aagttgcatc tgtcaacatc cgctgataat tacctaaccc gttaaatgtc
     3781 atattgacag ggggaccact ttgaaaagac gtgatcaaag ccaaaaccat cggaataaaa
     3841 accatgattg atatcaaaat gactggcaag attaaaaaag catgcccgtt tttgaataaa
     3901 tctttctgct tcattctttt ccctccaatt ctatcattga ttatctacat gaaaagacaa
     3961 gaaagacaat atctgtaagg cagacgtgat cttttttgtc ttttcgtttg tctgatgaaa
     4021 aagcgaagaa attcactttt ttaccaccga tcaattagcc aattgtgctt ctacttgttt
     4081 ttgtgtatct gctagtacct tgtctgtctc ttctccattg atgattcgat gaagagcttc
     4141 agctacgact gcttcaattg cataggtctc atgtccgtaa ttcacttctg gtatttcaga
     4201 tgtccatgtt gaaaaatcgt caaagatctt ttgaccactg taaaattcag aagcttcttg
     4261 ataagcttct tgttcattcg cgctaagcat cgtagatact agtccgattt ctttcgcaag
     4321 agtgcccatc agttcttcat tcgaagcaaa cgttgcttct aggaaatctt tcgctgaatc
     4381 ttctccagct actcctttga tcacatacca gccagcgccg ccaaggttag aagcttgcgc
     4441 tttttgcaga ttttctggaa gtgcaggtat tggtgcaatc ttccattttc cagattggtt
     4501 ttctgctcct tggatcgttg aagaatacca agcgccagtt gggctagaag ctactgctcc
     4561 tgattgtatc gcagttactc cagcattcca gtcagaggtt tgttcgacta gatcttcttt
     4621 caaaagagtg gcaaacaatt caagaccata ctttaaagat gcattgtctt ggatcgtgac
     4681 tgtctcccca tcttcagatg tataccattc tcctgcctgt tgcataatca tccttactcg
     4741 accaaggtca gaaggattga cttcagcgat tttctttccc gtcttttctt tgacatctcg
     4801 cgctacttga atatactcgt cccacttcag attctccata tcctcttctg tataacctgc
     4861 ttctgccatc agatccgtac gatagaagtt tgctgttacg cccgaatcga aaggaacacc
     4921 gtatatttta tctcctactt tgtttaccgc aaatttatac ggtgcaaaat gatcttcctt
     4981 tacaatagca gacaaattag cgaatgcatc tggataagaa gtcaaatagc cttgtatcct
     5041 ataatcttca atcaagacga tattgggcaa tccttctgta ttcccgctcg ctaaagcggt
     5101 gttcagtttc tgaacgatat catcctgaga catcgtaaca acttctatct ctgtttcttc
     5161 gttctcataa actttttttg cttcattcac tgctttgata ttgaatgtct catcccacgc
     5221 ccagccagtg actttttctt ctttgtctgc tgtactacta gcacttccgc atcctgttaa
     5281 gaaacctgat gctgccgcta aacaaacgac tccagcaatt aactttctga tgttcatttt
     5341 tatccgctcc ttttccatcc attagttgtt tacgtttgct acattcatca taccaaaatt
     5401 tgaaaacggt ttctttatta ttcagatact tcaaaaattt ctcctaaaat aataaaaaaa
     5461 tacaaatcaa ctatgacaaa aggaataatc ttagcatcta gaccaaagat cgaaatggtg
     5521 aggtttcttt aaaataaaaa tatccttgca ttttctccta gaaataagta tttttaaatt
     5581 acaagactat tcttaaggcg ggtaaaaaaa tgccaatacg cgtaattctt atcgatgatg
     5641 aacagacgat tttagatgga atgaaacagt tgatcaattg gtctgagcac gggtttcaat
     5701 taatagggac tttttccagt ccgttgaaag cgcttgattt ttctttggta catgcaatcg
     5761 atatcgtggt cacagatgta atgatgcctg
//
LOCUS       DAJSVF010000132         5562 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSVF010000132 DAJSVF010000000
VERSION     DAJSVF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.139"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(29..580)
                     /gene="raiA"
                     /locus_tag="ON550_002679"
     CDS             complement(29..580)
                     /gene="raiA"
                     /locus_tag="ON550_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR3955703.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(708..1427)
                     /locus_tag="ON550_002680"
     CDS             complement(708..1427)
                     /locus_tag="ON550_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR3955704.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1424..2737)
                     /locus_tag="ON550_002681"
     CDS             complement(1424..2737)
                     /locus_tag="ON550_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3955705.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2818..3138)
                     /locus_tag="ON550_002682"
     CDS             complement(2818..3138)
                     /locus_tag="ON550_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3955706.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3246..3533)
                     /locus_tag="ON550_002683"
     CDS             complement(3246..3533)
                     /locus_tag="ON550_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955707.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3660..4304
                     /locus_tag="ON550_002684"
     CDS             3660..4304
                     /locus_tag="ON550_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR3955708.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(4363..5070)
                     /locus_tag="ON550_002685"
     CDS             complement(4363..5070)
                     /locus_tag="ON550_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3955709.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5290..5460)
                     /locus_tag="ON550_002686"
     CDS             complement(5290..5460)
                     /locus_tag="ON550_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955710.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         1605 a         1168 c          957 g         1832 t
ORIGIN      
        1 gttttcgttg ttatttaagt aaggctgatc agtctgtttc aatcagtccg tattttccat
       61 ctttacggcg gtaaacgata cttgtaccat ttgtttctgc atcttcaaaa ataaagaagt
      121 tatgtcccag catgttcatt tgtaagactg cttcttcact atccattggt ttcaatgaaa
      181 ggcgtttcgt acgtacgata tctaattcag aatcattttc gttttcgtcc tcttcattga
      241 ataaagcagc agcatctgca gtattcatgc ccgtttcacg ggatttacga ttaatttttg
      301 ttttgaattt gcggatttgg cgttccaatt tatcaacaac caaatcgata cttgcgtata
      361 aatctggaga agtttcttcg gcacgtaata caagataagg taatgggatc gttacttcca
      421 cttttgctgt tttttctgta taaactttta agttcacatg cgccgttgca tcgggtgcgt
      481 cactgaaata gcgttctagt ttgcctactt ttttctcaac gtagtcgcga atcgcttcag
      541 ttacttcgat attttcgccg cgtacattat atctaaacat aaaaattacc cctttcgtct
      601 accacaatca agaaggagaa ggaccactct ttccctcttc ttaattctat tatatcgaac
      661 tctaggttat ttgcaaatta aatcgctatc aaatttccac atctaattca tctcgccaaa
      721 gaaatggttc ggatgatttt cggctgtgcg acctgcaaac aatctgctgc gtggaaaaga
      781 gtctgtcctg ttgtatagac atcatcaata atcaatattt ttttattttg tatcttagaa
      841 aaggtgactg caagttcaaa aggttgaggc atctccaatc tttcctttcg cgtgtttttt
      901 gcttgagcca gcagatgctt ctctcttttg agtagcatct gaaaaggaat tcttgctgct
      961 ttcaacatct cgcttacttg attgaatcct ctttctgttc gccttttttc agataaaggt
     1021 attggacaat agatgtatgt cgggtattct ttttttatct gcgtccattt atatgcaaag
     1081 gtgcctgcta gtcggatatc tcccataaac ttgtattgat tgagccaggc cttcatattt
     1141 tcatcgtaca caaaaaaggc acggtgctga aaatcatagg tcggatattt cttcttccat
     1201 tcttgacagt cactgcagta aacgggaata gactttactt tctttttcgt actctctaga
     1261 ttgaaatgga ttggtttttg acagccagaa cagcctcttg tttccaataa atgaaattgt
     1321 gcgcgacaaa ctgaacaagt ctgaggtaat ctgtatttcg aaacaccaat aatctcgctg
     1381 aatgttaaat tttggaagat atctttactg cagcaactgc acctcacagc atcttcttcc
     1441 cttcttcatt catttctttg atttcctgac aagcttttcg aatagctgca gttttttctt
     1501 catagaaaaa gtatacttga ctgttcgtat aaggcccttt acgatcggct cttcctgcga
     1561 tttgtaccaa agaagattta gtaaaaaccg gatgatccgc tcctaaaacg ataacggata
     1621 ttctttcaaa tgtgactcca cgttcaagga tcatcgtggt taacagtata tcatattttg
     1681 cttctcgcat tttctggact ttttccgccc gctgattgtc tcttgaagaa acatctgtga
     1741 tctcatatct cggaagagcc tcttgaagtg tggctttcaa acgatgcatc atcgtgatac
     1801 ttggacaaaa taaaaggata tggttttctt taattaattg atgaatgatc cgcaccaaag
     1861 attttatctt cttcttgttc ttgcattttt gtcgccaatt attcatgaaa accaagacgg
     1921 gttcaggaag caaacgccga tgaaagcgta aagcaagttt gtggatgtca aacgtctgct
     1981 tgatttcctt tagtaacttt ttatcaggtg tggcacttaa gtagatgaat cgtccgtctg
     2041 gtttaatcgc tgtttgcaca gcatgagcta atcctgcatc cccgttataa ggaaacgcat
     2101 cgatctcatc gatcactatt aaatcaaagg accgataaaa acggtatagc tgatgaatcg
     2161 tacagataac aaaagaagta aaggtataag ccgttgctgt attcccatgg agcaacgtga
     2221 ttttttcttc tggaaaaaca taacgatagc gtaaaaacaa ttcattacaa acatcgactc
     2281 gcggtgtaca aatcgctact cttcctccag cttttaaagt ttccactaaa ataggataaa
     2341 gcatctctgt tttccctgca cctgttacag cccaaataag atgatgtctt ttttctgaat
     2401 ggcaaagtat tctctttgag atttccgttt gtttttcagt aagctgtcct gtccaaaaaa
     2461 agacgacttc tcgaggtttt gcttcaggct gatcaaacaa atataaatct tgttgagtgt
     2521 cgcatcgccc taaatataga caatgtgtac aaaaataacc gtaatctgca agctgtccat
     2581 caatgacgga cgtcctcatt ccacagcgat gacaaagaat ccagttgccc aaacgttcca
     2641 ctgctgcgat cttctcataa tttggtaaat ttacctcctc acagtctatt gcttcaatat
     2701 ctctcgtcag aagccttcta ccccaaaaat gattcatatt cttcgcccta aaaaaatata
     2761 cgaaaaaaca agcgatctat tttttggatc gcttgttcga tagttatact gttctattta
     2821 ttcacctatg agcgtgattg cttgatctaa cactttttcg ccattcatca taccataatc
     2881 agccatattg atgacatcta aaggaatgcc ttttggttcc agacgtttct caaaatcagc
     2941 tttcataaag cgaacttgtg ggcctaataa caacacatcc acgtctttat ttgcaaggtt
     3001 gttatctgcg tcagaagcgg acacagcgaa aatatccgag tcgattcctt ttgcttcagc
     3061 agctttttgc atttttgtta ctagtaaact tgtgctcata ccagccgaac atacaagcat
     3121 gattgttttt ttagccatac ttttctctcc tttttaaaac aaactatgca aacgttttct
     3181 tcgcgtctgt tactctatta tataaaaaga aagaaatttg tcaacgtttt ctaaacgcgg
     3241 tttatttaat caaatagcca actagatttc actaatttat tgaacacgac atattctttg
     3301 tccaaattga tgacaactac ttcatagcct gcatttatcc agccataggc acctttttca
     3361 gggtgtttat aatcatttgc ccaccaatct gtactttccc ttgctgtttt gggaagtccg
     3421 ttactgggaa acagcagttc gtcaaattgg gtaaaggtca acgttacttg agagccgcca
     3481 ttgttcttca aataatgcgt aattgcatcg tactttttca ttcgttcctg catccccaga
     3541 tccacctgcc tttgtcttta ttatcgcata ctacagcaat tgcgcaaccg ttacagtgtt
     3601 gaaacaacct agaactttat ttatactgaa taaagaaaat gaaaaaaaga ggtgacaaga
     3661 tgatcgagcg ctatcgtaca ataaaagaaa atggacaaag tgagattgag atcaaaaaat
     3721 cacgctttat ctgttctctt agacgaatag aatcagagga ccaagcaaaa agtttcattc
     3781 aagaaataaa aaaagaacat tggaaagcga accataactg cagcgccttc atcattggag
     3841 aaaaaaatga aatccagcga agcagcgatg atggagaacc aagtggaacg gcgggcgtac
     3901 cgatgttgga agtcctcaaa aaaaatcaac tgatcaatgt agctgcagta gtcactcgat
     3961 attttggagg aacgaaactc ggcacaggcg gattaatacg agcatacact catgcggtct
     4021 ctcaagcatt gtctgacatt ggaatcgtag aaggtacctt gcaacaggag atctctgtga
     4081 tgatcacgta tccgcaacta ggaaaattac agaattatct tgaactccaa cattttttta
     4141 taaaagaaat ccaatatact gatcaagtta gcgtcatctg tgctgtagat gaaaaaagtg
     4201 ctgaccactt taaagaatcc atcattgagc tgctgaacgg acaagtccaa tttgaagagg
     4261 gttctgtttc ttatcacgag caactgataa ctgatcatat ataaaaacag gtgattctga
     4321 ttctctttta tttctaataa ggaatcaggg atcacctgtt ttttattttt ccaaataaaa
     4381 ctcaaaacga tttcctacgt attgcgtacg cacatactca aacggcgtac cgtcttcaaa
     4441 ataagaaatc tggcgcaaac gaagaactgc atctcctcgt tttacctcta aatagtcagc
     4501 aattttctca gaagccaaca cagcagtaat cgtttgattc gctggaccga tctttttccc
     4561 gcctttttct tctagcgtgc gatacaacga ttcagtaatc tctgctttgc tgtagccatc
     4621 gatcagcttt tgtggaacac tggcaacttc aaaacaaata gggacatcat ctgcgaaacg
     4681 aatacgttcc attcgcaaga tcgtgtcatc tttactcaag tttagttttt ccatttcact
     4741 tgagcttggc gaagtcaaga aataagagat tgtccgacta gacggtactc gattttgtga
     4801 ttccataatt tctgtaaaac ttgttgcacc agacattttt tcttgtactt tttctcgcgc
     4861 aacatacgta cccgaaccga tctttctttc taagattcct tcatctgcca agttttgaat
     4921 tgcttgtctc aacgtcatac ggctgacatt gaatttcact gctaattctc tttctgaagg
     4981 aagacggtct ccgactttcc atataccctg ttcgatttca ttttttaatt ggtcatgaat
     5041 ctgtatatat acaggtaatt gattcgacat aacgtacacc cgtcctttcg tttcttctat
     5101 tataactggt atatacctga ttcacaactg aaaaagataa aggtttctat ccatcaacag
     5161 aggatttaga cacacatcgt tctccctact cctattgtca gaaacaattt tttagatatg
     5221 ttcaacctag aaaataacaa aagaccctaa aatcgatgaa aatgccgact tatggatctt
     5281 ttgttattat tagcggtttt tagccatcca tttttttgta tgattcgttg ggcgttttga
     5341 ttttacctgg accgcttttc cgcctttttt cggaatattc acttcaatat tgtatttttc
     5401 tgatttgacc aaatcatata acttttcagc tgcttcgtct actttttctt tccatttcat
     5461 gtggatccct ccaattcttg tagttaactt aactctatct taattcagca gaatcttcaa
     5521 ggaacaaggt agcaaagcaa aaaaaagagc tctcgaacta ac
//
LOCUS       DAJSVF010000133         5479 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSVF010000133 DAJSVF010000000
VERSION     DAJSVF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.140"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(41..407)
                     /gene="ssrA"
                     /locus_tag="ON550_002687"
     tmRNA           complement(41..407)
                     /gene="ssrA"
                     /locus_tag="ON550_002687"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(578..1351)
                     /locus_tag="ON550_002688"
     CDS             complement(578..1351)
                     /locus_tag="ON550_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3955711.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1495..1845
                     /locus_tag="ON550_002689"
     CDS             1495..1845
                     /locus_tag="ON550_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR3955712.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1826..2542
                     /locus_tag="ON550_002690"
     CDS             1826..2542
                     /locus_tag="ON550_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3955713.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2542..3990
                     /locus_tag="ON550_002691"
     CDS             2542..3990
                     /locus_tag="ON550_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3955714.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4060..4569)
                     /locus_tag="ON550_002692"
     CDS             complement(4060..4569)
                     /locus_tag="ON550_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955715.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4559..5284)
                     /locus_tag="ON550_002693"
     CDS             complement(4559..5284)
                     /locus_tag="ON550_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955716.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1862 a          955 c         1095 g         1567 t
ORIGIN      
        1 ataaggattt aagcatctag cgaaatctgc tgaaatctca caatggagac ggcgggagtc
       61 gaacccgcgt ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag
      121 gattcacttt acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt
      181 tttcaactta cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag
      241 cacatgggcg atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag
      301 ttgttttcgt ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg
      361 aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag
      421 ggttacgtgt atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa
      481 aagcgaatca tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg
      541 attttcttaa gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca
      601 atgcgtggac aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta
      661 atccgtcata ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat
      721 cattcggacc atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca
      781 gacgtttgac acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac
      841 cattccgata aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag
      901 ataagatcag caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa
      961 catctgaatg aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga
     1021 ttttctgatc gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt
     1081 tttgacattc ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga
     1141 aagaaccatt catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt
     1201 ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca
     1261 tagcttttgc cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat
     1321 caaagaaagt gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag
     1381 tttatcttaa caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt
     1441 ggttcagtca acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt
     1501 tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca
     1561 aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat
     1621 tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca
     1681 ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt
     1741 ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat
     1801 gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt
     1861 ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga
     1921 tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact
     1981 agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc
     2041 aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg
     2101 cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc
     2161 tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagtagt
     2221 ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga
     2281 agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt
     2341 tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg
     2401 ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca
     2461 aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt
     2521 cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttctgcgga
     2581 atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg
     2641 attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg
     2701 caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg
     2761 ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac
     2821 gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa
     2881 tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga
     2941 caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga
     3001 gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg
     3061 aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt
     3121 gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat
     3181 ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat
     3241 gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa
     3301 aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca
     3361 atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac
     3421 gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg
     3481 gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca
     3541 gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca
     3601 ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa
     3661 atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt
     3721 actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg
     3781 tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa
     3841 aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg
     3901 cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt
     3961 ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg
     4021 atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg
     4081 atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt
     4141 ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag
     4201 ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa
     4261 ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg
     4321 agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac
     4381 atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat
     4441 gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt
     4501 atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc
     4561 aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga
     4621 atagactaag aatgagacag catccactga gcccaataag agtttgggca aatcccttaa
     4681 gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca
     4741 tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag
     4801 tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta
     4861 cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa
     4921 taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc aattcctgct
     4981 tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata
     5041 aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg
     5101 tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt
     5161 gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac
     5221 aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt
     5281 tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc
     5341 tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag
     5401 aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag
     5461 gagcagcgac aataatttt
//
LOCUS       DAJSVF010000134         5168 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSVF010000134 DAJSVF010000000
VERSION     DAJSVF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.141"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..134
                     /locus_tag="ON550_002694"
     CDS             <1..134
                     /locus_tag="ON550_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3955717.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(276..2084)
                     /gene="pepF"
                     /locus_tag="ON550_002695"
     CDS             complement(276..2084)
                     /gene="pepF"
                     /locus_tag="ON550_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR3955718.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2260..3258)
                     /locus_tag="ON550_002696"
     CDS             complement(2260..3258)
                     /locus_tag="ON550_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR3955719.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3484..4137)
                     /locus_tag="ON550_002697"
     CDS             complement(3484..4137)
                     /locus_tag="ON550_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR3955720.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4469..4867)
                     /gene="spxA"
                     /locus_tag="ON550_002698"
     CDS             complement(4469..4867)
                     /gene="spxA"
                     /locus_tag="ON550_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3955721.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1558 a         1063 c          788 g         1759 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataatacaaa aaagatgttg gtgaagttca ccaacatcaa
      181 aaagtataga gccttaaaaa aatacgtcga ctcccttaca agagagccga cgtatttttt
      241 tcgtttctat tcgagaaaat caaaaataga aagccttata aacgatcgat caattcttct
      301 aattcattta gacgttgttc aaacatcgac attgcatctt caatatatgc agcctgtgtc
      361 atatcaactc ctgctttttt cattacttcc acaggataat cgctgttacc tgcttttagg
      421 taagccaagt agttctctag tgcctctggt tcctggttta gtattttctt cgccagtgct
      481 gaagcagcag aaaagccagt cgagtattgg aaaacatagt aattataata aaaatgcggt
      541 atccgtgacc attcgaattt aatttctggg tcttcttcga ctgctggacc ataatatttt
      601 gcattcaatt taccataact atcacttagg tattcactag tcaatggcac acctttttca
      661 tcttccgtat gcatgaaatg ctcgaattca gcgaattgtg tctggcggaa aactgttcct
      721 ttaaacccat ccaagtaatg gttgagtaca taagcccgta cacgagggtc tttttctgtt
      781 tccaataaat actccgttag gatattttca ttcgttgtcg aagcaatttc tgctaaaaag
      841 atggagtagt cgccgtacac ataaggctgg ttcgaacgag tgaaataact atgaacacta
      901 tgtcccattt cgtggacaag cgtaaatagc tgatccagcg tatcatgcca attcaataaa
      961 atatatggat ttgtgtcata gcttcccgaa gaataagcac cgcttcgttt ccctttattt
     1021 tcgacaacat cgatccaacg ttcagagaat gctttttcta cgatggccat gtattcttca
     1081 cccattggtt tcagtgcttc taaagctttt tcttttgctt cttcgtacgt aaaggtaatt
     1141 ggagcttccc ctaagaccgg tgtataaaga tcatacatgt gcagtttttc cacttctaat
     1201 aaacgtttcc gtaattccat gtatcgatgt aacaaaggca aatgcttgtt taccacgtct
     1261 accaaagtat cgtatacact ttcaggaata tgattattgc tcaaagacgc ttctctggca
     1321 gagctgtaat tacggacttt cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta
     1381 gaagcaaatg tatttctaaa ttgttcgtaa acactgtaca accccttaaa tgctgcttcg
     1441 cgcacccttc gatccgtgct ttctagcaac tgaccataca cgccatgaga taattggact
     1501 atttcaccat tttctccttc aatcgttgga aaaactagat ctgcattatt caaaacagca
     1561 aatgtatcac ttgaagcatc aaagatttca cctgctccag caagaagaga ttcctgctca
     1621 gcagataaga catgggctcg attatctact atttgctgga tataatggcg atagacttcc
     1681 aattttggtt cttctttgaa atattgccaa atctggtcat ctgacaattg caatatttct
     1741 ggttcaaacc aggaaacggc ttcactaact ttcgaaaaca gactgcttgc tcttgcataa
     1801 agcgcttggt aatctgtatt tccagtgtct tgatcgtttt taaggtgcgc atatacataa
     1861 atgacttcag tttggcgata aaccctcaat acgaattcaa tcgcatttaa aaattgagaa
     1921 gcgccttgat caagtgttcc tttgtgtttt tcagattgtt ttaactcttc tgataattcc
     1981 aaatatttct catcaaactc ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa
     2041 ttttcaggca attcttctcg ttttggcaac tgctttactt ccatgaaatc cactcctttt
     2101 cttcccttag ctctatctat caaaatagat catagtctaa catccacatg tcacagcatc
     2161 taacttttct actcattcat gtgtctgctt ccatcaattt tagcataact aaatcttgtt
     2221 ttacatagaa aaccgattcc tagcctattt ttatacttac tattttttca aagaagacaa
     2281 gtgtatgcat tcgttaaaaa actgctgata gaccatctct tcatcaatca taggaaaaag
     2341 ccattccctt ttatgttctt ttatttggca aagccatgag ttgaaatcag gaactttctg
     2401 gttttccaag gcttcttcgt acaacattcg ataaagaaaa acctgttctt gtaaataaaa
     2461 gaaaaaccga cttttttgat agatccacga tgatagatgc aaaagatgtt tatgacgcaa
     2521 gtagcattgt tcttgtatgg atactgtatt cttttgcttg tgaataagtt ggcgtgtcag
     2581 ccatttcttc ccatcttcac tcactttttc tatttttttc atttggttca ttttaatttc
     2641 gttactagtg aatatttctt taaggtccct actagaaaaa gtccagcagc tactgcaaaa
     2701 ggaaatatgc cctgatacag tctctgtaat atgatgatac aaataaagct tttgtttctt
     2761 gagatcagct ttccagcaat gcactcctct ttttctatta tagtagcagc aacttttttc
     2821 tatcaatgag aattgcgatt gtccaagaaa agaattacca agtatccacc atacttggta
     2881 actgttattc aaataatttt gggttctttc aataaatctc tggtgagaca gtctagaaca
     2941 ttggatttct attgctagat tcgccaacaa aatatccggc ctttgccgca acctcggcaa
     3001 ataggcttcc aaaaatactg gttctactga ctgtcccagc caatccatga agcattcctt
     3061 taactgtaag tgctcttttg attctctttc actaaagacc gaacagttat ctgatttttg
     3121 atgggcaaaa tgagcacatt ttttctttcc ctttttcaaa atcactcttt ttcgacagcc
     3181 acgacaataa tatttctttc ctttttctgc atgtttagca aaacagcact tattatattc
     3241 attcattgca agcagcattt ttctccctct gaacttattt acgtctaaga atttatttac
     3301 acgaaaaaag agggcgtgaa aaagctattc tgcatcaagt aataagaaca gctaaacaaa
     3361 aatagttccc aatatttttc gtttggttgg gcttgttacc gcagatgcaa gcttttgaac
     3421 acatttatac gaaaaaaaga ctgcgacaca atttttgtca cagtctctta ctataatgct
     3481 gatttataca aaataaaatt tcgttcgctc gatagcatta cgcgccatca gacattgtcc
     3541 atattcattc aatgtttctg gtgtacgatc gctcttttca gcaaactcca aaattcgagc
     3601 aatctgattc tctacatccg cttggttgag attttccatc cagaagtgaa cgactaggta
     3661 atagcgctct tcgaataaat acaaatcagt ccaagcagac tctagttcga catctgttgc
     3721 aagttggatc acttgttcaa aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc
     3781 tagggcgctt ccagataatt cttcctccgg atcgctgcca ataatctgct gacgaagaaa
     3841 gtcactcaca tcttctgaat caacagtaga atcttctgat tgagaaattt cttcatccgg
     3901 aagattttta ctgataaaca gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac
     3961 agcttcgctt cctcggaact cctcttcgat atcaacttct tccaaaatac tataaaagaa
     4021 attttcgatt tctttgtgat tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa
     4081 atcttcatga ccaattagta cacggatcgt attttcatta atatgttcca tttccatttt
     4141 gctacacctc actttgccac aatgtttaat atagattcat tgtagcgaaa tcagcttaaa
     4201 tgtaaagaaa aaaaatctcg ctgttaagcg aatttaataa aaccagcgga tttttcaacc
     4261 tttcaagcta tcttatttaa ctataacatt tttggcagac gaatactata tagccgactt
     4321 ttcagctaat tttccctctc tatttttcgt gcaatcatca tcttctacta cctttttata
     4381 catctaatca agaatacttt ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat
     4441 agtcttttcc aagtttcgta ttatccttct ataatccagc catcaattgt gcttgacgta
     4501 attctaattg tcgtacatcg cgcggtagga accgacgaat ttcatcttca ttaaaaccaa
     4561 cttgcagacg tttatcatca atcatgattg gacggcgaag caatccagga ttgttttgca
     4621 caagttccaa taattcttgt aacggcagat catctaaatc catgttcagt ttttggaata
     4681 ctttggaacg tgtcgagatg atttcttctg ttccgtcttc tgtcatttgt aaaatcgctt
     4741 ttaattcaga actgttcaat ggttctgaaa aaatattacg ttcaacaaat ggaatctgat
     4801 gttcttgcaa ccacgcacga gcctttctgc aggaagtaca acttggggaa gtatataatg
     4861 ttaacaacgt gcaccactcc ttttcgtgat aagaatcact tttttgtaac agaagatcat
     4921 aataatctcc catctgtttc tattatacat aatcaacttt taaatagcta cctttttttc
     4981 ataatttttt taaaaaaatg aaagttatct aaggttaata tacagtaaaa ccagcattct
     5041 cgcttttttt atcatttaaa aattagagtt ttcaaatcta attcaaattt tctaaacata
     5101 tgaaacagcg tataaaactg tactattatt ttatctcaaa agctaaatga aaccgatttc
     5161 ttaagatt
//
LOCUS       DAJSVF010000135         5136 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSVF010000135 DAJSVF010000000
VERSION     DAJSVF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.142"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            317..1102
                     /locus_tag="ON550_002699"
     CDS             317..1102
                     /locus_tag="ON550_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3955722.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="ON550_002700"
     CDS             1115..1966
                     /locus_tag="ON550_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR3955723.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="ON550_002701"
     CDS             complement(2090..2542)
                     /locus_tag="ON550_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955724.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="ON550_002702"
     CDS             complement(3007..3375)
                     /locus_tag="ON550_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR3955725.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3525..4007
                     /locus_tag="ON550_002703"
     CDS             3525..4007
                     /locus_tag="ON550_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR3955726.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4044..5054
                     /locus_tag="ON550_002704"
     CDS             4044..5054
                     /locus_tag="ON550_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR3955727.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         1713 a          827 c         1035 g         1561 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaag tttacgacat
     2161 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 attgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatctgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
     3481 atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt gacttggcaa
     3541 agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc atttatacaa
     3601 atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct
     3661 tattcttaaa agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa
     3721 cggatttcaa agcattgggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac
     3781 acatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac
     3841 agattccgat tattattgat gaagagctct tattaaatga aaaacttgtt tttgcaccag
     3901 gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac
     3961 ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag
     4021 tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga aatacaacga
     4081 ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag
     4141 ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc
     4201 aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc
     4261 aagatgaaaa tatccaaatg atctttccgc cttggggagg agaattattg attgagattc
     4321 tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta
     4381 gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat ggaatcaacc
     4441 ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa agtgtactga
     4501 aaacaagaga aggagaatcg attctacagc aaccttctga aaagtatcaa aaagagtggc
     4561 cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag
     4621 tcagcggaga tgcagtaagg atgaagggac gattattagg tggctgtatt gatacgatca
     4681 gtcatttaat cggtacccct tatggagatg tccaatcttt taaaagaaag tataatgatg
     4741 gaaagccgat cttgtggttt ctagaaaatt gtgagctatc gtcagttgat ttaagaagat
     4801 cacttgttca gatgaaactg gcaggatggt ttgatggcag ctcggggatc atgttcggta
     4861 gaagtgcagc agaggatttg ttagattata aaatgaagga tgtatacgaa gatctgtcaa
     4921 aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga
     4981 cattgatcaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc
     5041 aaacgtttat ttaaaaaatt ttttcctgcc ttggagttta tcaaagacca acaaatctaa
     5101 atagaaaaga aaatgaaaga agctgacagt attttg
//
LOCUS       DAJSVF010000136         5051 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSVF010000136 DAJSVF010000000
VERSION     DAJSVF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.143"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="ON550_002705"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="ON550_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR3955728.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="ON550_002706"
     CDS             complement(2886..3191)
                     /locus_tag="ON550_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955729.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="ON550_002707"
     CDS             complement(3336..4541)
                     /locus_tag="ON550_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR3955730.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1620 a          914 c          667 g         1850 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct t
//
LOCUS       DAJSVF010000137         4946 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSVF010000137 DAJSVF010000000
VERSION     DAJSVF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.144"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            44..379
                     /locus_tag="ON550_002708"
     CDS             44..379
                     /locus_tag="ON550_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955731.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(636..2219)
                     /locus_tag="ON550_002709"
     CDS             complement(636..2219)
                     /locus_tag="ON550_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955732.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2341..2925
                     /locus_tag="ON550_002710"
     CDS             2341..2925
                     /locus_tag="ON550_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955733.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2936..3181
                     /locus_tag="ON550_002711"
     CDS             2936..3181
                     /locus_tag="ON550_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955734.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3187..3909
                     /locus_tag="ON550_002712"
     CDS             3187..3909
                     /locus_tag="ON550_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955735.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4040..4783
                     /locus_tag="ON550_002713"
     CDS             4040..4783
                     /locus_tag="ON550_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955736.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4789..>4946
                     /locus_tag="ON550_002714"
     CDS             4789..>4946
                     /locus_tag="ON550_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955737.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDR"
BASE COUNT         1805 a          662 c          818 g         1661 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta
      481 tatcaaaata aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt
      541 atatttatta ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat
      601 atacgctata aaagtgcatc ttgtgttata aaaaattatt ttattttgtg aacataattt
      661 aacaattctt tctcggaatt gatttctttt cttaaatagt cttcagactt attaataata
      721 ttatctatta tgtctagata catatcactt ttcgattcag ctaattcata cagttttttt
      781 aattctttat cctttaattg aaaaccctga tccatagtca ctgcatttgg atatagaagc
      841 ccactataat gtttacgtaa attcacttgg aatttctgat tataaaagtc taacagatta
      901 gaaataaaag gcggaaaatt actgtttttg taaaattgat tatttccatt taggttgtat
      961 agtaatttgt taaattgctc ctgatttaat ttattgtaaa tccatggtaa tacttccata
     1021 tccttatctg ctaacctgaa tgaactagca tcggtaagat gttcgatcat aaaatctatg
     1081 aacatctctt tttcaccaaa atataaatat tgattatata ttgtttctaa tgttgaagat
     1141 ggtagcgaag aaactctcat tttttgctgg agtataggat caactagact atttaagtga
     1201 ttttgtaatt cttcattgtt gtttcctaga actgcaaggg aacgtaaacg gtagtaatat
     1261 tctatttctg ttagtacttg ttgtttcggt ttaaaatcat ttattgatat ttgtaaattt
     1321 tgaattgtct cgtctgaaaa atcatgttgt aattctggat gggtagctat aaaagtaagt
     1381 aaaaagaacc cttttgtata cgtgatctgt tgattttcac caagtgattt attaatttcc
     1441 tctcttgatt cagctgtgaa cctatacttt gcaatttctt tttttagaat atttaaatag
     1501 aattctttat tatcaaaaat tagggcatta agtagtctga aagtactttc cctatctcta
     1561 tctgcttcct catttccata agataattga aaaacaaact taaaggctgt cttacatagg
     1621 ttttctttgg ctttttcgct tagtttattt agatagctat tttttagtgc agagtgaaat
     1681 tcttcaggtg taacagacat agcatgtatg cgacttgcat attgtataat ttcgatcgtt
     1741 actttttttc ctaatattgg attttttagg aatataattt cataggactt ccgtataaca
     1801 tcctgacaag tttcttttgt tatatctctt aattcccaag tattaccgtt gtaagtgact
     1861 acaggatgag ctgaataatt tctttgatcc ttaatatatt ttaaatcctc atattcatag
     1921 ctatcaatta gctcagttga actataagct ttttctagta gttccatttc ccattttgga
     1981 gagtttggat cactttttct tagtctagct atatcttcta aaattttctt agaagtatta
     2041 tcctcgtaaa tatttgctaa atcagataat ttatcaatta aatctaaaat aagcgcactg
     2101 taattgacag ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct
     2161 aataatggaa taattctagg atttttaact ttttttattt catcatctaa tataaacata
     2221 taaatctcct ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga
     2281 catatttatg ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa
     2341 atggatttga aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg
     2401 gctgatattt ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat
     2461 ttaatcgggt acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt
     2521 gctaacaatc aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat
     2581 gtgatccatg aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca
     2641 atgacaacgg atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg
     2701 cgtgatggtc aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa
     2761 atttcttttt gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt
     2821 aaaacagcct ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac
     2881 ccatataatt ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa
     2941 aaaaattata aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat
     3001 gtattttact gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct
     3061 attatttagt ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt
     3121 gttttattgg atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg
     3181 aataaaatga aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataacc
     3241 cttacagcat gtggtacaac taatagtgct aataataaca aagacacaaa gtctagtagt
     3301 acgatggtta ctaaaaacag cactacatcg gcatcaacaa caatgtcttc aatgactgaa
     3361 aataatgctg aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggatgaa
     3421 gtttataaca actttattaa tacagtaaaa aatgatgaaa attatgggaa tgatccattg
     3481 caaaataaaa atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca
     3541 gaatcaaaaa tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat
     3601 gatattacca taaataaacc agcttacgag tattacgcag ttgctgtaga tattactgat
     3661 ggtgtacctg agtgggtaca aaacattcct ggtctggctg atgagaacaa tcaatatcat
     3721 acgccaacag ttgcagtagt aacaccaaag aaagttaacg gcacactata ctgggatgta
     3781 atgggaatga caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg
     3841 gatttgggag catataaatt atatcaacaa tcagagcctg ttatggacga tcaatccaaa
     3901 gttaaataga tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa
     3961 tgatttctga aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga
     4021 ttataaagga gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa
     4081 gaatcaggtt ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact
     4141 taccggttac aataatggtg caaatgggaa gtggttaaag gcaacaagta ttcgtgaact
     4201 tagaagtgaa gtagaaaaaa ttaggcttgg tctcacaagt gaagaaatag ctaaactgaa
     4261 gggaaataat tcttttgtaa ttgaaaaaga aaaggaaaaa catctacttt atcgtgagct
     4321 gaaaattgaa gaatactatg aaattgattt acctgaaaaa ttaagaatta ttgatcaacg
     4381 ttcagaagaa aaatggaaaa ctgaaatata tccaagctta aatgactaca agacatcagg
     4441 aaagcccatg tataactaca caggcttttt aacctacaca caatacaaat atgcctttcg
     4501 caaattaaaa ggcattaaac aagctgaaat tgccctgtat gagatgaaaa aagtggatag
     4561 attgaaattg attcaagatt tagtgcaaaa tacatggaat caagcgattg tcgagctaga
     4621 aaaagaaaat gggaaaattt taactaataa atatctgatc gtaaatgaaa ttgaagaatt
     4681 aattggttta ttttcatgtg aaaaaggtga agtcttcttg aagtcatcac caacctattc
     4741 aattttggac agtccgtatc agtttataca ggcaaggaga tgagttagat gtctagcttg
     4801 aaagatgact atatccaagc aaaacaagag tatgaaaaaa tacagatgtt gaaatctgag
     4861 aaagtcgaat tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct
     4921 aaaactttag aggaatatga tcggca
//
LOCUS       DAJSVF010000138         4937 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSVF010000138 DAJSVF010000000
VERSION     DAJSVF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.145"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(55..684)
                     /locus_tag="ON550_002715"
     CDS             complement(55..684)
                     /locus_tag="ON550_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955738.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(681..2057)
                     /locus_tag="ON550_002716"
     CDS             complement(681..2057)
                     /locus_tag="ON550_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCR3955739.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(2137..3399)
                     /locus_tag="ON550_002717"
     CDS             complement(2137..3399)
                     /locus_tag="ON550_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955740.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(3412..3759)
                     /locus_tag="ON550_002718"
     CDS             complement(3412..3759)
                     /locus_tag="ON550_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955741.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(3779..4648)
                     /locus_tag="ON550_002719"
     CDS             complement(3779..4648)
                     /locus_tag="ON550_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3955742.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
BASE COUNT         1332 a         1004 c          709 g         1892 t
ORIGIN      
        1 aaagcctgtt ctatttgctt caaaaagttc tagcaatctt tttcacctct actgctactg
       61 ccaaacaaat ttagctttat catgccatgt ttcagacatt acttttttac cattttcgtc
      121 aaatatcacc gcttcccact ttgctgttcc atccggctgt ttacaaatat ctgcaaacca
      181 taggatcgtt tttcgatcgt tgaatttcct tactttcttc tcaaatttat tgatctgaac
      241 tctattttga ttgctgttcc aacctactaa taggcggtta tctgattctg taccatgaaa
      301 accaatggga acgatgtggg ttctatcaaa tgcttctttt ccttctggat agagcattgg
      361 tctaagtttc ctacgtttca agtcatctct tgcttgatac cgccatgccg attttggatt
      421 gaatttaata gctatttgat gcttgctttt gtcatggtaa aaatatgcga catcggcttc
      481 agctccaagc aacttttctc ttgcactatt tgattcaaac ggtttattca cttctaaaac
      541 cttcacccct aaaatgccat gatcaagcat ctgtttagga gttagtaccg cttttttatc
      601 tcttttcttc gctcgtttca caactacttc atctgtatat ttatatggag taagccatct
      661 taaaaaagaa ctgacgatga tcatttatgc acctgttttt ttgcttttaa cattttttgc
      721 atgataggtg aagtacttcc atttatttgt ttcttttcag atttatcagt tgtgatcact
      781 acgttttctg agccttttgg atcattagct ttaggtttgg ttttagatgc aatcggctgt
      841 ttattattcg tgttgtttga ggtttgcggt tcaggatcag cttttcctaa tgcccttaaa
      901 actttttcta gtttttctgc ttgcagtttt tctagtgttt tagctccctg ctcgactttt
      961 tcttctttgt tcttttcaag cttaatagca aacgcactct tcttttcaat tgatggctct
     1021 ttagatgatg gatttgctaa aggtgtgttc aaatttcttt tagcagattt tcttacagga
     1081 gctttaccaa ttccaccaaa atcctcgtct tcaccgccct caccacgagt ttcagctacg
     1141 attgctcggt aatcaacata ttctggttct ggcatagtat ttctaaggtg attgaagatt
     1201 gtttcaattt ctttgttact gatgtaagct ccctgcgctc taaccatact tccatcaaca
     1261 agcttaataa gcatatctcc ctttggactt agactttctc caccgccttc atctaataca
     1321 atcctacttt ctaaatcact tgctaccata aggcaaacac gtgacggaaa attggctttg
     1381 ataataccag tgacaactgt tgatcgagga gattgtgttg ctaagataac atgaatacct
     1441 gctgcccttg ccatttgtcc taattgaaca atcaaacctt ctacttcttt atgtgttcca
     1501 accaactgtg aatattcatc aactaataag atcacatagg gaaatctatc taatccattt
     1561 ttttcggcat attcgttata tagcgtgatg ttttgtaacc ctgctttttc catcagtttg
     1621 taacgttcat gcatcaagat cacaagatat tcaaaaaaat cataagcttc atccatatct
     1681 gtaattggat cagccaacat gaacggcaat tttttgtatc ttccaaattc aacccttttt
     1741 gggtctacaa ttcctatttt taattcatca ggcgttgagt gacacattac tgtaacaaga
     1801 atttcattca gtaatactga tttccctgat cctgtcgttc ctgcaaccaa catgtgtggc
     1861 atttcgctga tgcaaaaatg agcaattgtg ccatcaggct taattccaaa aatagcatct
     1921 gttgggtctt tagcctttgt gttcaacata ttgacactga attttccatt aagctttata
     1981 ttaggttctt tatctagcaa aatagattca gctttttcgg ctctcttttt gactttctct
     2041 aataatttca tagccatttc tattcctcac ttttctcggt acttttctgt ttgtgaactg
     2101 gtagtttgta ttttctgttt ctatttgtga tgtcttctaa aacacgctat tgatcattga
     2161 tggtacatca attggaatac gatcattttc tcctaatggt aaagtaattt gaatacgccc
     2221 tgcgttgatc cgcacgatta cacctgatat atgcaagtaa gtttcaattt cttctttcat
     2281 tgagcttagt tgaatttttc tggtacgaga taaagtaaac aaatattcaa tagatgtatt
     2341 cccaacgtgc atatctgtta gacttgcagg tacgtcagca gaagatagat aaagttctaa
     2401 agattgcttt tgagtttcgg catattttct agctttttcg gtttggtttt ggatattttt
     2461 ggaattatcg gcaaagagat tgattggaaa agaaaattgt gaatttgaaa ctgcgattga
     2521 agatgttgct ctttccgttt ctttgttttt ggtacttact ctttccttac gttctctttc
     2581 aatatttact gatgctcgga aaatgaaata atcgcctgaa acttcatctt ctttttcacc
     2641 aaatttggct cgtccgcttg ttgctcttgt caggatcgac cctaaatctt gaatttgtcg
     2701 taaaactgtt tctctaactt tcttcccttt tggttgcggg atttttacga tatattctcg
     2761 aatatcttca ttgacactta gatcacgtct tgtatgaaca gaaattttga tatttttaag
     2821 cgttgatagc acatcggatt ctaattgcgt tttgtttcgt tttctttttt tgttgttcgt
     2881 ttcatctcct ataatcttgt ttggatcata cttcacatcc atagcttcta gagcttctct
     2941 tttcaacctt ctactttcca tatcatcaaa aaatggtctt tgttctccat agtgatgcct
     3001 gaacatcaaa gtaaccaaat atccaacaaa gaataccgtt cctgcttcta atactaaaat
     3061 agagatgtct accaagtgta gctttgcaaa aaagctttgt acattatatg aataacttaa
     3121 ataattggga aaaaacgcat tgagtaactc tttaaacatc tcttttaatc ctaaatcatc
     3181 tgttccgtta acatcattta acgagacaag aatacttact aaggtatcta caagcagttg
     3241 tacaaccttt gttacccaat gcaaaatata tatgccaatt gcaccaacta ccaatgcacg
     3301 tccaaccaga tatactgatt tagaaaaact ggctacagga taatattttt ctccatcgct
     3361 tcgcttatat tcctttaatt caatggattt ttcgctcatc tgaatatctc cttattttct
     3421 ttctgatttt ggttcatcgt cacttatgct gtcctcacac atctgcgtat ctttaggaat
     3481 gattactctg atttcgtcta acatttctct ctcaatgatc atcgcccctt ttgtactaac
     3541 agttttctct gataattcaa cacatttccc tatttcagaa tctggatcaa aaatagcctg
     3601 ttctacgtca aaatgattct tagctacata cacgatactc attgtttcta actctctagc
     3661 aactgaatat ttacccttct tcagaagata gtagccattt ccttttttta ttcgatttgc
     3721 aactgattta tctaatccat tgatgcttat tgtttccaat tcttcacctt ctcaatttct
     3781 aatctcttac ccaagtacca ttttgatagt tgatgttgat tcctttttga ttgttaaata
     3841 cataaacatt aaggtttacc gtttttccat tatcgttaac tgagaaagct tcgacatgag
     3901 aaccacgagg aactaattca ttccctttat aaattggatc aactttatac aatacttttt
     3961 cattcttatt tttatagatt gcatcacgta caatcgtttc tgagtaagcc atccctccat
     4021 tctgatcatt tttcccaaca ttttgcgctc tcgttccaag tgttaaattg tgtacctcca
     4081 tatctcctcc tagcgaccat gctaatgaat gacttttgtt atagagataa cctttatagc
     4141 cttctagctg agtgatttca ttgtttgaat ctttgccgac ccaagtatct ctctcttgat
     4201 caaatactcc atccttgtat tctcctgcaa tatggacaga acttttgaaa gatggacgtt
     4261 ccacattttt agatgagtga gaatttacag catcataggt gatcaacgct tcaacatctc
     4321 ctgctcttcc aagttgatct aaatctgcat aatttaccca aaaggtttga ctgccttttt
     4381 ctgataatgc ttcatggtct tcttttgtca gtgtatattt cccatcatta atgtttacga
     4441 tattatttgg atatttgttt ggatcataac gtatccctaa aaattctttt gattcactgt
     4501 ctgctttatt acctacctga acactacttt tgatctgaaa atcatgttcc tgagtactgt
     4561 tattctgaaa gtaacccttg ccactaagac caaccgcaac taaaaccaaa attcctaaca
     4621 acgatcggct aaacttcttc tttttcatac caccattcct tttattgtgt tatcataata
     4681 agataaatat ataataaact atattgtaaa ttttgtcaaa tgtattttta tttattccca
     4741 ctataaacct tgatatatag ccaatttaaa aaaggatggc aaaaaattat caagttaaaa
     4801 aaataaattt tgaaccaaat tatttttaat aaaatagcta gtttttttat caaaaaaaat
     4861 agagcttttc agctctattc agactgtaga caaactccta aatttggagt gttgtttgca
     4921 gttttttgtt ttatact
//
LOCUS       DAJSVF010000139         4880 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSVF010000139 DAJSVF010000000
VERSION     DAJSVF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.146"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(503..844)
                     /locus_tag="ON550_002720"
     CDS             complement(503..844)
                     /locus_tag="ON550_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3955743.1"
                     /translation="MKPNEIVTVYAAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(834..1115)
                     /locus_tag="ON550_002721"
     CDS             complement(834..1115)
                     /locus_tag="ON550_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR3955744.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDKPVDVLDTDEKLEEWFNEA"
     gene            complement(1222..1806)
                     /locus_tag="ON550_002722"
     CDS             complement(1222..1806)
                     /locus_tag="ON550_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955745.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1823..2062)
                     /locus_tag="ON550_002723"
     CDS             complement(1823..2062)
                     /locus_tag="ON550_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955746.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(2081..2329)
                     /locus_tag="ON550_002724"
     CDS             complement(2081..2329)
                     /locus_tag="ON550_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955747.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2344..2910)
                     /locus_tag="ON550_002725"
     CDS             complement(2344..2910)
                     /locus_tag="ON550_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955748.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2911..3456)
                     /locus_tag="ON550_002726"
     CDS             complement(2911..3456)
                     /locus_tag="ON550_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955749.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3482..4249)
                     /locus_tag="ON550_002727"
     CDS             complement(3482..4249)
                     /locus_tag="ON550_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HCR3955750.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1370 a         1054 c          611 g         1845 t
ORIGIN      
        1 gcgaccgttc gttcgtcttt tcctccgtgg gactcaccca ccgcaagcgg ttggttgagt
       61 tgtggctcct attcaaaatt cactaatatg ttatttttgt tttctattca agtgggtaac
      121 gttcgtttgc aagcaaactc tctctatgta acttgccagt tacatcagtg gtaacgctac
      181 gtcgcaagcg acttgctcta atgaaactac cgtttcattc tgcctgttcg ttcgtagcaa
      241 gctacttcac tcactgtatc cgtctgaatt attgttattt attactattc aatatccgtc
      301 agtcatttta tttatttaca tacattaaat ataaaaatgt atcttataat tcgtatatta
      361 ctaattatca ctatttaaca atacttaata ttttcttaat ttttataatc acgtatatat
      421 ttacgtgtat tttaatatac ataaatacgt ttatataaac gtatatctta atatacatat
      481 aaacaaaaaa agaaaagcct tgttagactt ttctttgtaa tctctcgata aacctattta
      541 gctttttcgt atcaatagct gatagcatac caaccttttt aaatgatatg ttctctcggc
      601 ttttgggaat agcctttaaa gaccctacgt caatccatga ttgcttcttt agacctactt
      661 tttcccattc tgcaatagga aaatacactc tctttatttg gtctgattta ttctcatatt
      721 gacttgtgat tccaaaaaat tctactctat cttctttatc actaaccacc aaaataggtc
      781 ttcttttacc accattttta tctgtaaatg cagcatagac cgttactatc tcattaggct
      841 tcattgaacc actcttctaa cttttcatct gtatctagaa catcaactgg cttatccata
      901 gttagctgtt gcaatctgat tccttgcttt tcctcttcgc ttagctttat ctctactgga
      961 aactcacctc tagcgaccac ttgcttataa aggattgtga ttgctgttgt tggatttaac
     1021 cctagctctt ccaatatcat ttctgcgttt tcttttaaat ccttgtctat tccgacttga
     1081 attctgcttt tttcttttgg attttttacc ttcatttcgt tcacctcttt tgagtataat
     1141 atactcatta ttatatccat taactacatt tatgattata ttatactctt aattagctac
     1201 tttgcaaaca atccaatcaa atcaagattc tactttactt tttatacgtt ctttcatctt
     1261 ctggtatgca acctctactc tttggacatc tatatccaat ccacttacaa ccgtacacat
     1321 accaatctca tcgctatccg acactttatc atattcttct tcggtgattt caataggcat
     1381 atacccatca ttaaagcata tctcatcatc tgaatagttt tcccattcct cttcatcatc
     1441 ttctgaataa gtgacaaccc tatctgcccc ctgttctacc atatcatcgt aactatcagc
     1501 aacatactgc gcttttaatc ctttcagttg taagtcatat aaaccataca acctcttact
     1561 atcttgttca ctgaataaaa ttctttgacg ttctaaaatt aattctggtt catcttccac
     1621 ctcttcatct ggttctgtaa aataccattc gataaaataa tatctaatat cctcattcga
     1681 taaactttca aagtccatat tctcctcatc aaatccatca aaagcgccac tactttgcat
     1741 accattcaac caattaatgg ttctcaatct tgcgtattca cttaatacca tcttattctc
     1801 attcatggtt cttcacccca tattaattat tttcaaattg acaaatcaat ttattttgag
     1861 ttaacaaaac taatagctgt tcactatcaa aaagaacctt acaagattct ttttctattc
     1921 tctcttctgg catgtaatag ccaatctgtt ttaacatctt atctggataa cctttttgtt
     1981 tggcttttct caaaagctct tcatcattcc caacataata ataagaacac cattgcatac
     2041 ctttactttt gaattttgtc atgttgctac cctctttcgt ttattgaccc aaccatgaaa
     2101 aaatatattt tgctaaatct tctttttcta tcccatataa aacattctct aaatccaatt
     2161 tatactcttc atcaacaagg ctttctgtat tgtcatcata aaggacatag actgcataat
     2221 ttctttcgta aaaggcatta gcaagacttc tgctaatacc taccattcct ttccatgtat
     2281 aaccaaaatc aatcaaatct tcatgatcta taaaatccat tccttccatg ctgattcaca
     2341 ctcctattct gctgttgtgt aatatgcaac gcaatcatct gtccctacta accacacaca
     2401 ccaatcttca atttcatcat cgcttctctt actcaattcc tctttggtac aaaaaactac
     2461 ttcaccattt tctgtttcca tctcataatc ttgtagcatg tattcaaacc aacttctttc
     2521 ggttggaaat tctatctcgt ggcaactctg gcaaataaaa tcaccatatt cattgattcg
     2581 gtagcaacct tcatcttcta caaattcatg attacaacaa acacatctag tacctgttag
     2641 gacttcaccc tcaatcgttg ttctaacggt atcttctctt gctacgtgtt cactgctgac
     2701 aagccaatca atgattttct ttgccaattc ttcatcagaa tcggcaaagc ctttaaacgt
     2761 gtttctcttt ccttgaaagt agtatttgca acttgcggta gtttctatgg ttccataact
     2821 tttttggaat aattcaatca tttcaccatc aatcactttt tgacttttcg cttctaaatt
     2881 agggaactgg tcttttcttt tttcaaacac ttatttcacg ctctcttctt ctaggtttat
     2941 tccataattt tctagcaata atctgtatcc cttaataaaa tctttcgtag caaactcttt
     3001 tccgctttca caaaacttgt ttagtcctat ctgttgaccc tcaaaatcaa acaatcgcaa
     3061 tctggtttta tcatctagac taaatccatt ttcatcgaac accatctcta aatgagcata
     3121 gccatcattg ttatctgaaa acttcatcac atcttcaatc gtttcttgat aaggttcata
     3181 gcgcccacca aaccctgttt catcataagc actcactcca gattcatagg tgacaaaatt
     3241 gataactcct gtacttctat caaagattcc ttttgctaat tcttctcttg attcatctcg
     3301 taagtcatat gctttattga ttaaatatat tccttgattc aaaacgtcca tcaacatgaa
     3361 tcgaccatat ccccactgat aatgaattga cacgcttact ttcgttttgc tgttaccata
     3421 tttgactgta attaacgctt gtgttctttc gcccatcttg ttttcctctt ttctctaatt
     3481 tttataaaga accattttct tgaaatgagt aatagcttgt gtttttactt gttacgatga
     3541 tatggtctaa caattcaata ccaattgttt ttcctgcgtt tttcaaattc gtagtaaaac
     3601 ttttgtcatt ttcacttgga aattcatatc cgctcggatg attatgtgat actgcgattt
     3661 ttgctgaatt gtttagcaaa gctagttgaa aaatctctct tggtatcgct actgattgat
     3721 taattccacc tttgaaaact tcgctataag caatgacctc attctttgta ttcagacaca
     3781 tcactagcaa agattcacta gcattgtttc caattaagtt agtagcaaag tttgctagac
     3841 caaccgagct tgtgactcta taaaaacttc tatcttcact gatatatttt tgttcgattt
     3901 tcaggacatt cttaatcaat ccttcacctt cattctcggt cttgttttca acttcaatag
     3961 attcaagact gctatttccc atatcagaac ccaacaaagc atttctgaaa tccaatacct
     4021 tttcaaagtt atcagtcata accatttcat aggtgctatt ccattcattc aaatatttct
     4081 ctttgatgag aatgaacgct ctcggtttac ttctgccaat tctgatattt tgtgtatcgt
     4141 gaaaaatata attatcaagt aaagtaccat cgtacttttc atagttccca ctgatatttg
     4201 ttaacttgtt taccatttct tgattaactt ctaaaattct atctctcatg ttgaacgctt
     4261 ctttctgttt ttgatttttg gtttttcaaa attctttttt gtttggattg ttttagaggg
     4321 aggtggtttt ttcttcatcg ctatttggtt ttgcatttta tgtttcttcc tttcgttcat
     4381 ttatttatct ttttcgcctg tcttgccttg tcgggagtga ttagaatgcg ttcttatctg
     4441 ccggattaat acaagtgttt aagtaaatta ttttcgtaac gcttgcgttt agaaaaaaat
     4501 ttacgccact tgtattaatc caaagatgag ataagctcta aacacttctg acaaggtatg
     4561 acaggcgaaa aagataatgg gcgtaaggaa ttataaatgg caaaaccaaa taaagagcga
     4621 tgaatttcaa tgctttcagg cgtagcttga aagcctaaaa gtcttgaaga atgaaagact
     4681 ttttaccttc aaagcagacc tagtaatctt aaataggcga tggctattta agattacggt
     4741 gttgacccac taaataaaca gaaatggaac cgatcaagca aaatcggact aggcgatggc
     4801 taggacggtt tgcggtggct ttaggaaacg ttcatggtga gatgagcata tctagcaatg
     4861 tcgggctaag ctatgcttag
//
LOCUS       DAJSVF010000140         4872 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSVF010000140 DAJSVF010000000
VERSION     DAJSVF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.148"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..360
                     /locus_tag="ON550_002728"
     CDS             <1..360
                     /locus_tag="ON550_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3955751.1"
                     /translation="IETVNYVTNFQREMRKVEAMTVLATQSPQEMVPENMSSDNFSKL
                     KKVFEFSSTKIFMRMDESILRHIEGLVGKSMTNSELESIPQQNQGDAVINFGSKETIR
                     VHFSPNKRQLKLFDGGK"
     gene            360..1559
                     /locus_tag="ON550_002729"
     CDS             360..1559
                     /locus_tag="ON550_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3955752.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1575..2219
                     /locus_tag="ON550_002730"
     CDS             1575..2219
                     /locus_tag="ON550_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955753.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2230..3036
                     /locus_tag="ON550_002731"
     CDS             2230..3036
                     /locus_tag="ON550_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955754.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            3059..3288
                     /locus_tag="ON550_002732"
                     /pseudo
     CDS             3059..3288
                     /locus_tag="ON550_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3309..4382
                     /locus_tag="ON550_002733"
     CDS             3309..4382
                     /locus_tag="ON550_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HCR3955755.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            4426..4695
                     /locus_tag="ON550_002734"
     CDS             4426..4695
                     /locus_tag="ON550_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955756.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT         1758 a          721 c         1031 g         1362 t
ORIGIN      
        1 attgaaacgg taaattatgt cacaaacttc caacgagaaa tgcgtaaagt agaagcaatg
       61 actgttttag ctacacaatc cccacaagaa atggtgccag aaaatatgtc ttctgataac
      121 ttctcgaaac tgaagaaagt ctttgaattt tcttctacga agatctttat gcgtatggat
      181 gaatctattt tacgtcatat agaaggatta gttggtaaat caatgacgaa ctcagaatta
      241 gaatccattc ctcaacaaaa tcaaggagac gctgtgatca attttggctc aaaagaaacc
      301 attcgtgttc attttagtcc gaacaaacgt caattaaaat tgtttgatgg aggaaaataa
      361 tggaaaacca aaatgaaagt ttaatcaaac agtatgttaa gcgtcgtgca aagcggcgct
      421 tatttttgtg ggtgtttggt acttctgcag gtttgattac tatattgatc actgtgtttg
      481 tgaccctttt tctaatactt gcggcaggtt ctattgataa ttctgattca agtagtggtg
      541 gcgaagcttt tacaggagaa tattcggaag gcttacccat ttataaagaa atcaaaggaa
      601 gaggcccttt ttctgatgag attgcgcaat atgctgtagg tgcagcagtg aagtataaat
      661 tattaccaag tgtgatcctt tcgcaatacg gttatgaatc agcgtttggt acgtcggcgt
      721 ccgcaagaaa tgatctcaat tattttggga ttacgtggtt tgatgggtgt ctgtttccta
      781 aaggaactgc gcgtggaatt ggtggcattg aaggtggctg gtacatgaag tttcccaact
      841 caaaagctgc cttctcctat tacggcttta tggtagccac acaatcaaat tttaatgctt
      901 gtgtcggaaa taaaagccct ggcgcaagct tattgatctt aggacgagga gggtatgccg
      961 ccgcaggaat tactgaagac agtgcctatt ataaaaattg tatgtctatt attaatacaa
     1021 ataaattgat ggagtacgat gagtttgcga ttaaacactg gagagaaggc ggcggaactg
     1081 gtggtacgat tacaggaagc tggggcaatc cattcccagg aagtagctta gataaaaatt
     1141 cgttcagtgg aggacaactt tttgggaaaa atccaggagg agaattccga cctaacgggt
     1201 tccatgatgg actagacttt ggttcagtcg atcatccagg aagtgagatt catgcggttc
     1261 atggcggaaa agtcgtgtac gttggcaatc ctggtatttc aggtttgggt gcgtgtgtga
     1321 tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga atttgcaaat agtacaggaa
     1381 attctcgagt gaaagtaggc gatcaagtga aagttggtca agtaattggg atacgagata
     1441 cagcacactt gcatttagga tttactcgaa tggattggcg tcaagcacaa ggacatgcat
     1501 tcattgatga tggtacatgg atcgatccgt taccattttt gaatagcagc aaaaaataga
     1561 tcaggagaaa aaatatggaa aataaaacct taaaaatcgt cttaacagct gcaattagcg
     1621 ttagtttaat cggcaatatt gcgttgtttt ggcagcttaa agaagaacaa gagacaaata
     1681 cctataaaat tgaagaagtg gctaaaaacg cgtctgataa gctcaaaaaa aattcttctg
     1741 aagcacaggc gaagattgac aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg
     1801 atcagctgaa gcaacaagat ggcacaaaag caaaagagga agtaactgaa gcacaaagaa
     1861 agacggcaga agagttcggt caattggcca ttgataaatc attaagcaga aatgaattaa
     1921 cggaaaaact aaaagatgtc gcaacccaag aagtgatcga caaattaagt cctgtagacg
     1981 atgatcacca acatgatgat tacggaagtt actcttttac attaggtgac gtgcaaactt
     2041 acgctcaaac aagcagtgta aaagaggaac aaaattttgt cgtatttgtt gattatacaa
     2101 tcggcaatcc tcaattcaaa gatgtcaaac cacaaaaaat aaaaggtgga ttaactgtca
     2161 ctgaaaaaca agtagatggt caatggaaag tcacagactt tagttatttc actcgttagg
     2221 aggaagaaaa tgagtaagaa aaaaggagca agacaacgtt ttaaaaaacg aaaaagttat
     2281 cttagatggc tggggatctt tatgattctc ggctttttga ttcttgctag tccgatttac
     2341 acacataaaa aggtagacat accagatgaa gtaagcacga acgcattttt aaacacgaag
     2401 gaactgacga tggtttcaaa agaaaaagga gcgaaccagc aattagtttt tgaatttgcg
     2461 atcgacgatg aagatcagag cttattaaaa gaactagcta atcttaatta tcgtgtagaa
     2521 gtaaagacag ctaaagggga ttatcaagca ataaaagcca aagtgatcaa ggtgtctgat
     2581 gattattttg tagtgaagtt agcacatgta ccagaagaat atattgctat gcgattaact
     2641 attattcctg aaaaaattga tccaaaggtg gatatgcaag aaccgcaaga tctgattttt
     2701 tatattcacg aagataaggt caaagatcac gtgaaagacg aagattatga acaacatgcg
     2761 atcaattaca aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa acaaattgaa
     2821 tccttccaag caaccattga tttgaatgaa gaattagtga agaaactaaa agatcaattg
     2881 ccttatcaag tcgcagaaga tcaggaaaat actgaaaata agattaatag ttaccagcaa
     2941 gaaattgaaa catcgaaaac gcaaatgaaa gatcaagaag agagcattca aaaacttcaa
     3001 gaaaaaattg atcgtatgac aaaagaaaat atttagaaga ataggtggag taaaagaaat
     3061 gaagcgagcg aacttggtcg gcaaagatac tttcgctgag cagaaagtat tgattaaagc
     3121 ctatctcaaa gttgtaaaag ctaaagaaat atgtcaagaa gtgaattatc taacacaaca
     3181 atatctgagt tgaagaatgg tagaaagagt ttagatacct taaaattcag atcatttgaa
     3241 gctctatata tagctgctaa aaaatatttt acagctgata ataaataaaa aattaaggtg
     3301 ggttctatgt gaaaaaaacg tatcaaaagt ttttatggat tagctatatc gtcctattta
     3361 tcgttgggtt tctcgtcagt gtgtccgtga tcgtcggaac gctttttctt ttgttctctg
     3421 acgaagatct ggtacttaag gggttaatga gtacgttatc gtgtgtcatg ttactttatt
     3481 tatgtataca gatcaacgaa attatgcccc ctcattggca agaaaagaag aaagaattta
     3541 aggaagaaat cgaaataaaa tccaatgact ggattgaata cacatggctt cctctgaaaa
     3601 gaaatagcaa acgaaaggaa ttttgggcat tgcaattatg gccaatagga aaacttttta
     3661 tttatatggc ttcacttatg gttactttaa tcggttattt ggtggtggtc tatggggtgg
     3721 cctattactt gttaagtgag ccaaaacaga tcgcggcgtc aaatattttc acggcagtaa
     3781 tgacattaat gtgtgtcatg tgcttttcta tttttagtta cttactcgtc caatttatca
     3841 ggcaaaattt aacaatgagt aaacttttca aaagtttatt ggacttactt atcttaggca
     3901 gtatgttaac gactctaaca gatcggttcg aacaatctga aaaaacggta gaggggataa
     3961 agaacttctt aacgacgatt gatcaattta taaatggata tatgtatttg tttgaagtag
     4021 gctttattct cactttgttt ggttgtgtat ttataaaact cattaaaaga tcaatcgaaa
     4081 aagaaatggt tattgatcta attccgacgc tgagcggcta tgagtattac gttgattttg
     4141 gaaagtatga ggagttgatc catacggata tcgttcacac gagtatccat gaagtaaccg
     4201 ttaagggcat cagatctact ttaaaagatg gacaggagac gaaaacaatc gaccgaagaa
     4261 aagaggtcta caatttttat aaagttgaag caagtctacg atatggcatt gggaaattga
     4321 ggttagatgg gagtaatatt caacaaaaca aaccatatgt tttaggaaaa gtggtgattt
     4381 agaacttcct atgaaagatg aataaataag gagaatcttt tgaaaatgat tcttctttac
     4441 agaattgtgc ttatagttgg cacggagtta aaaatcttaa agaaggagca gttattcaaa
     4501 gtgaaagaac atcataatca gttactatgt attaaaacgg gtacgatgaa cattcaatac
     4561 cgaacagaat atttagaatt aacgaatcaa actgattttt tgtcgattcg agagcaacat
     4621 aagcaagaaa aattaatggc tacttatagc caattgattg atgagtggga acaattcaag
     4681 gaatctaaaa cataaaattc catttaaaca ccttattttt taaagagcat aaggaaacgt
     4741 gtacccactt tcaaaataaa gtgggtagat aggctgtgcc tatcacgttt ctgaaaaaaa
     4801 taaatgaccc caaaattttc aatttatgtg taggtttatg ggtagcaaaa atatgaatgt
     4861 gggcagcaaa aa
//
LOCUS       DAJSVF010000141         4852 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSVF010000141 DAJSVF010000000
VERSION     DAJSVF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.149"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(207..1169)
                     /locus_tag="ON550_002735"
     CDS             complement(207..1169)
                     /locus_tag="ON550_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3955757.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ON550_002736"
     CDS             complement(1171..2610)
                     /locus_tag="ON550_002736"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR3955758.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ON550_002737"
     CDS             2795..4741
                     /locus_tag="ON550_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR3955759.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1550 a          826 c          794 g         1682 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tg
//
LOCUS       DAJSVF010000142         4760 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSVF010000142 DAJSVF010000000
VERSION     DAJSVF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.150"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            315..2159
                     /locus_tag="ON550_002738"
     CDS             315..2159
                     /locus_tag="ON550_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3955760.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSSTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2214..3680
                     /locus_tag="ON550_002739"
     CDS             2214..3680
                     /locus_tag="ON550_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3955761.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3717..4559
                     /locus_tag="ON550_002740"
     CDS             3717..4559
                     /locus_tag="ON550_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3955762.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1555 a          765 c         1022 g         1418 t
ORIGIN      
        1 gttggaagat attcttagaa aaaagacaaa acttttagaa cacgaaaact atattgaacg
       61 aataaaatga aaaaatgatt aaaaaatcat tttattattt acgtttacat tctaatatgt
      121 taaattgaat ataacttaaa caggcatgtg actggtaatg caggcaggac ctaaataagt
      181 tgaataacaa tgtatgcgtt tcaatacacg tttgccttgt tgtcaattta tttagtttct
      241 gcctgttttt tgtgttgagg atggccactt cgactcaaaa gttcatcact caaaaaagaa
      301 aagagggaaa caccatgaat tacaaacaaa cagcagaaga agtattgcag tacatcggtg
      361 gggaagaaaa catatcccat ttggagcatt gctcaactcg tttacgtttt acgttaaaag
      421 acaacagcaa ggtaaatatg agccagttag aaaaagtgga tggcgttatg ggcgttcgcc
      481 aaaatgtgca atgccaagta atcattggaa acgatgttgt tgaagtgtat gatgaagtga
      541 aagcgttatt aggtgacaat gtttcaagta ctgaagaagc accaaaacaa aaacaaaaat
      601 taggttcagt tctattagat tttattattt ctattttcca acctttaatt cctgcgattg
      661 caggtggagg agtattgaag tccttgttgc tactagcttc tatcttaggg ctgattagtg
      721 accaatctca aacctatcag attttaaatt tggtcggcgg ggcaccgctt tatttcttgc
      781 ctattctggt ggcaatgact acggctaata aattgaaagt caatcaattg gttgcagtat
      841 ctgctgtcgg tgcattgatc cttcctgaaa tgacagctat gttggcggaa ggtgctacat
      901 tcttatcttt tggattggat aatatagcgt atgcatctca agtattccca gccatcttaa
      961 ctgttttatt ttatgctcaa cttgaaaaat ggtttacaaa agtttcacca aaaccaattc
     1021 gtattttctt tgtacctatg atgagtttat tgattacagt tcctattgct ttgttgcttc
     1081 taggaccatt aggttataat gtaggaacag ctttctcttc tatcattgtg tctttgtaca
     1141 atcaatttgg atgggttgca accggagtct tagcagctgt actgccatta atggttgtaa
     1201 cgggtatgca taaagcaatg atcccttacg ctgtttcatc catgagtgaa ttgggtgcag
     1261 aacttcttta tctcccggct tcattggcac acaatatttc agaagctggc gcatgctttg
     1321 ctgtcagtat caaaacaaaa gacaaaaaat tgaaatctac ggctgtttct gcgggtatat
     1381 ctgcattatt cggaattact gaaccagctc tttacggggt aacgattctg cacaaacgtg
     1441 ttttatatgc cgttatgttt tctagcctga tcgggggagc atttgcagga atcgttgcac
     1501 ttcaagcctt tgctttagtg ggtcctggga ttgcaagtat cacgatgttc gctgacaaag
     1561 ctgacccaat gaatctggtt tgggcatttg cagtatcggg gatttcattt gtggttgctt
     1621 ttgctgcagc gttggttctt tataaagata aagaagttgt caatgaagtt gaaactaaga
     1681 tggaaacggc cgttgtttca gatgctgttc tttcaagtcc cgtgaatgga aaagtaatct
     1741 cactatcaga agtaaaagac actgtttttt ctagtggtat ggtaggtgat ggagtagcaa
     1801 tcattcctga agaagggata ttagtagctc ctgaagatgg agaaatcaca atggtatttg
     1861 aaacgaagca tgctattggt ttaaaaacgg ctaatggtgc agaacttctt tttcatattg
     1921 gtattgatac tgttcagtta gaaggaaaag ggtttgaggc atttgttcgt tctggtgaaa
     1981 aagtagccaa aggacaaaag ctgattgcat ttgatattga tttaatcaaa gaagcaggat
     2041 tcgaccccac agttatctgt gtggtaacca ataaggataa ttacactatc cgttcattta
     2101 acctagaaaa attggtaacc aatgatagtg atctcttagc tatcagtccg atagcttaaa
     2161 cacaagatag tgaaaaaaat atattgaatg aaggaataga ggagaataga ataatgacaa
     2221 acacaacaag ttttccaaaa ggatttttat ggggcggcgc gatagcagca aatcaagtag
     2281 aaggtgcttg gaatgtagac ggtaaaggtc tttcagtagc ggatatggct acttataaac
     2341 caaatttaga caacaaagat tttgctgctc atatgcatgt caccctgcag tctataaaag
     2401 aagctagtga agacaaatct gataaatggt acccaaaaag aaggggcgtt gatttttatc
     2461 atcactataa agaagattta gctttatttg ccgaaatggg ctttaaaacc ttgcgcctct
     2521 ctattgcttg gactcgcttg ttccctacag gagaagagct ggaaccgaat gaaaaagggg
     2581 ttgcatttta tgaaaacgtc tttaaagaaa tgcaacgttt aggaattgag ccgattgtga
     2641 cactatctca ttatgaaatg ccattagctt tagcagttaa atataatggc tgggtggagc
     2701 gtcgagtaat tgatgatttt gttcgcttcg ctaacgtttg ttttgatcgc ttcggcaaat
     2761 acgtaaaata ttggctgaca tttaatgaga tagacagcat ccaccgtcat ccatttacaa
     2821 cagctggaat tatccaagag aaaagcgcag aaggtcaaga agctcaagat atctatcaag
     2881 cattgcatca tcaatttgtt gcgagtgcgt tagtgacaaa agatgctcat gaaaaaattc
     2941 caggaagcca agttggttgt atgttaacga aattgatgac ttatccatta acttgcgcac
     3001 cagaagacgt tgagctgacc ttgaaaaaga acttagaaaa taatttttat gcagatgttc
     3061 aagttaaggg agaatatcct gctctaatcg aaaaggattt ggaaaatcgg ggaattcaga
     3121 ttaaaatgga agatgaagat cgagaaattt taaagaaaca taccgtagat tttgtttctt
     3181 tcagctatta catgtccatg gcagaatcag ctgatccaaa cgcagaacgc acaccgggaa
     3241 atactgtttt aggggttaag aatcctcatt tgccttcaac agattggggc tggcaaatcg
     3301 atccaaaagg tttgaaaatt tcgttgatcg aactttatga tcgttaccaa gtaccgttga
     3361 tgattgtgga aaatggtatg ggagcagtcg atcaggtagt agatggtaaa atccatgatc
     3421 cgtaccgcat cgactacttt aaagcacact tccaacaaat gaaagaagca atcgatgaag
     3481 gtgtagattt gatagcttat actagctggg gaccaattga tttagttagt gcaggaactt
     3541 ctcaaatgtc taaacgatat ggatttattt atgtggatgc agacgatgaa ggaaatggga
     3601 catataaccg cttgaaaaag gattcgttca actggtacaa agaagtgatc gaaacaaacg
     3661 gtgcatcctt attcgagtag tctcgatctt tttaagcagc gataggggga taatgagtga
     3721 tttcaataaa aaaagtattg aattcaagtg tggtactggt tgaagaaaat ggcaaagaaa
     3781 tgattgcatt aggtaaaggg ataggctttg gacagaaagc tggggatgaa atatcaggag
     3841 ataaagtaga taaaatattt ctgccttttg aagaaaagaa atcttcacaa ctattagagt
     3901 tagttgaaga aatacctatt gaattttttg aaatcactcg tactatcgtt tttgaagcag
     3961 aaaaaggatt agataaaaaa ctgaatcctg gagtctattt aaccttaaca gaccacctgc
     4021 attttgcagt tgaacgcgca caaaaagggg taaatattgc aaaccgtttg tactgggaaa
     4081 taaaaatcta ctatcccaaa gaatttcaaa ttgggaagta cgcactcacc cttttgaacg
     4141 agcaatatgg aattgatctt cctaaagagg aagcctcaaa cattgctttc catttgatca
     4201 atgcacaatc ggaagatcct gatagcggcg ctggattcaa gtatgcaaaa atgatcggcg
     4261 ggatcgtcaa cttggtccgg tattcgttgc agaaagatat cgatactaat tcgatccatt
     4321 atgcccgctt cattacacat gtacgtttct ttgtagaaag atattattct gatgctttgt
     4381 tggatgatgg tgaggaagaa ttctacaatc aaatgtggat cttatacccg tctgccatgg
     4441 aaagcgcagc taagatcaaa gactacattg aaaaagttta tcataaaaca attccgaacg
     4501 atgaagttgt atacctaggt gtccacatca atcggttgat gaaacatgct aatgggtgag
     4561 cgctcagtaa tactattgat gcttttaaaa tctttagaga tggattatag taaaaacaag
     4621 gtgatttcca gctgtttgtt atctataaga gacatttaaa aaggattaac gtaagataat
     4681 aaaaaatctc ggctctttgt caaatgatgt tggtaaagag aaatcttaga ataatttgga
     4741 cagaacagaa agaagaaaac
//
LOCUS       DAJSVF010000143         4693 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSVF010000143 DAJSVF010000000
VERSION     DAJSVF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.151"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            212..1939
                     /locus_tag="ON550_002741"
     CDS             212..1939
                     /locus_tag="ON550_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR3955763.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            1954..2304
                     /locus_tag="ON550_002742"
     CDS             1954..2304
                     /locus_tag="ON550_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3955764.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2396..3256)
                     /locus_tag="ON550_002743"
     CDS             complement(2396..3256)
                     /locus_tag="ON550_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR3955765.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            3348..3776
                     /locus_tag="ON550_002744"
     CDS             3348..3776
                     /locus_tag="ON550_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3955766.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            3938..4114
                     /locus_tag="ON550_002745"
     CDS             3938..4114
                     /locus_tag="ON550_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR3955767.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            4141..4587
                     /locus_tag="ON550_002746"
     CDS             4141..4587
                     /locus_tag="ON550_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR3955768.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         1604 a          810 c          978 g         1301 t
ORIGIN      
        1 ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc gcgtgaaatt
       61 tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat cctttcttga
      121 tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta tacttattaa
      181 gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt atgaacaatg
      241 ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga
      301 tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg caggtatgcg
      361 gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac aagcaacaga
      421 aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag gtgtcgctat
      481 cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca aaacactagc
      541 acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag agctttcttt
      601 tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc acaacccagc
      661 ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc ctgcagacgc
      721 agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc
      781 tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag tggacgctgc
      841 ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa caatggggaa
      901 agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc
      961 gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg tggcagatcc
     1021 agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg aatatgctat
     1081 acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg atgcagaccg
     1141 tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg ggaaccaaat
     1201 cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca ctttaccaga
     1261 aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa ttgcggaaca
     1321 ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg aaaaaatcca
     1381 acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa gttatggtta
     1441 tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt tattagcaga
     1501 agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc aaacgatttt
     1561 tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg gacaagaagg
     1621 ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga cagaatttgc
     1681 cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag accgtgaagg
     1741 aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat tggaagaaaa
     1801 tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt atattggtgt
     1861 aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag cgattatcaa
     1921 aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt
     1981 cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag
     2041 aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat tctgctatat
     2101 ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggagcagtct
     2161 gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag
     2221 ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt gaaccagaca
     2281 ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacgctg gagtgtgaca
     2341 acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa aatgattaat
     2401 cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct aaagcattca
     2461 aaataagcga agccgtttct tcaatagata aagatgccac attgatcact acacatccta
     2521 gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg atatcggaat
     2581 aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata ttatttaaaa
     2641 tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac agctgtttgg
     2701 gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta tttgctaaaa
     2761 acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca tctgcttcta
     2821 aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt gctttgatcc
     2881 gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt ggcttgatac
     2941 cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta aataattgat
     3001 gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat gtatgcagga
     3061 caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt tcatctgtca
     3121 cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata gtagcttgcg
     3181 ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta acgatttctg
     3241 tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta ttataccata
     3301 ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg aaaaaacaga
     3361 cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat actaaaaaaa
     3421 gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct aaagaagttt
     3481 ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac cggaatctcc
     3541 atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa atgaagtttc
     3601 gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt acagtgtgcg
     3661 gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag ctaggtggat
     3721 gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt caataaggat
     3781 ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga cggtcaaaac
     3841 agcattagat ataatattta caggacagtc ttgttttttg agatatattt caataacaat
     3901 tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag tcgttcgcaa
     3961 aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa aagcgggtac
     4021 tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac gcaagaaaaa
     4081 atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg agttggtagt
     4141 gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc gaaagataag
     4201 gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca gattaaagaa
     4261 ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat gaaacagcga
     4321 cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga aaaagtaaaa
     4381 agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga agaaattcga
     4441 caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga atttggcaaa
     4501 gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca agtgaatgcg
     4561 attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca gtgattgtcc
     4621 gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag aataagattc
     4681 tttcttctag ctg
//
LOCUS       DAJSVF010000144         4633 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSVF010000144 DAJSVF010000000
VERSION     DAJSVF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.152"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(246..749)
                     /locus_tag="ON550_002747"
     CDS             complement(246..749)
                     /locus_tag="ON550_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3955769.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1108..2133
                     /locus_tag="ON550_002748"
     CDS             1108..2133
                     /locus_tag="ON550_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3955770.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2480..3358
                     /locus_tag="ON550_002749"
     CDS             2480..3358
                     /locus_tag="ON550_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR3955771.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3834..3950
                     /locus_tag="ON550_002750"
     CDS             3834..3950
                     /locus_tag="ON550_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3955772.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1528 a          737 c          837 g         1531 t
ORIGIN      
        1 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
       61 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
      121 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
      181 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
      241 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
      301 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
      361 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
      421 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
      481 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
      541 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
      601 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
      661 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
      721 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
      781 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
      841 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
      901 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
      961 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     1021 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     1081 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     1141 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
     1201 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
     1261 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
     1321 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
     1381 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
     1441 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
     1501 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
     1561 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
     1621 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
     1681 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
     1741 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
     1801 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
     1861 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
     1921 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
     1981 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
     2041 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
     2101 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
     2161 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
     2221 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
     2281 ctatcaaaaa attttatatt tattctatca aaaaattttg tttatgatgg taactgaata
     2341 aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag
     2401 aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg
     2461 ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa
     2521 aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga
     2581 gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga
     2641 actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga
     2701 ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat
     2761 ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag
     2821 atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat
     2881 ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata
     2941 ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat
     3001 tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg
     3061 tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt
     3121 actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc
     3181 cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg
     3241 acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa
     3301 agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt
     3361 ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca
     3421 cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat
     3481 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
     3541 tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact
     3601 agctataaca atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac
     3661 gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt
     3721 taacaagttg actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt
     3781 cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa
     3841 aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac
     3901 gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa
     3961 acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt
     4021 gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag
     4081 aatctttctt ttcaaattga taccattccc taattccagt tatatagtta actacagaac
     4141 cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac
     4201 ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga
     4261 aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc
     4321 atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa
     4381 gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa
     4441 aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc
     4501 cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat
     4561 aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt
     4621 aataaagtat ttt
//
LOCUS       DAJSVF010000145         4590 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSVF010000145 DAJSVF010000000
VERSION     DAJSVF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.154"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..144)
                     /locus_tag="ON550_002751"
     CDS             complement(<1..144)
                     /locus_tag="ON550_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955773.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDR"
     gene            382..996
                     /locus_tag="ON550_002752"
     CDS             382..996
                     /locus_tag="ON550_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955774.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1161..1985)
                     /locus_tag="ON550_002753"
     CDS             complement(1161..1985)
                     /locus_tag="ON550_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955775.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2009..2164)
                     /locus_tag="ON550_002754"
     CDS             complement(2009..2164)
                     /locus_tag="ON550_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955776.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(2623..2880)
                     /locus_tag="ON550_002755"
     CDS             complement(2623..2880)
                     /locus_tag="ON550_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3955777.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2873..3142)
                     /locus_tag="ON550_002756"
     CDS             complement(2873..3142)
                     /locus_tag="ON550_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3955778.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(3273..3452)
                     /locus_tag="ON550_002757"
     CDS             complement(3273..3452)
                     /locus_tag="ON550_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955779.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(3833..4540)
                     /locus_tag="ON550_002758"
     CDS             complement(3833..4540)
                     /locus_tag="ON550_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955780.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
BASE COUNT         1451 a          843 c          632 g         1664 t
ORIGIN      
        1 cctatctaaa ggagttaatt cttggttatt atctactaca gtagttggtt catttgtact
       61 atcagcaaat gtaggaattg tagttccagt tacactaatt ccaatagtta aaatacctaa
      121 agataaaata aacttatttt tcattgtcta tcacctcgaa atcaaaaata agaaaacgtt
      181 ttcttaataa atcttaacag ataaatcaag ttatatacta gaaaattcat aaaatatatt
      241 tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa aatattgcca
      301 tttaaaaaga aaagtgacat atggcttcct cgtgtatgat tagggtgtat tttaaatagt
      361 tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc gatcgatcaa
      421 aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat tttcatcgag
      481 aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt agattacgtc
      541 cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa ttatgacgag
      601 atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat tactagttta
      661 ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa ggatctgatc
      721 atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg tcgccaggca
      781 caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa attatacagt
      841 gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa
      901 aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca aaccgtgtat
      961 agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct tttattatct
     1021 tctcatcttt aatttcctac atataattac tatctataaa attaagtctt actcagaaca
     1081 gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga gtctgttaat
     1141 ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt atctatattt
     1201 tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta tgtcgacaca
     1261 ttggttatac taattcctat agctagttct tgagactcta cttttctctc tagctccttt
     1321 gacaaagtga tttgagattt atttggttcc gttggagtca attctataga ttcttcataa
     1381 aaatctctat acataaagct atcattaatt ttataaaaaa aattaaatgt ttttagcact
     1441 gccatataac attcaaaaca ttcttctata gttggtttac ttccttgagg agaatgaacc
     1501 aatttattac gtttttctat gaaattttta atttcctttc ttatttcttt cggaaactca
     1561 atattaaagt accctttaaa catgctccct aataatttgt ctacaggggg agattgtaaa
     1621 ttctcaaata ttagagttaa atcaggtttc tcttgttgat aaaattcctt tacccctatt
     1681 tcacaggctg taattatcga aataaaccta tctctagtat ttgatatatt tttacttctt
     1741 ttaaggagta taagagaaaa ggggatactt tcgtgtccat gcatgtaatt aactaattgt
     1801 tgagcttcag aagttatatc tctaattata tagttaacgg aattaggaag tgtctttaac
     1861 tctaatgaaa tttctatcca attactatct tctaatctgt atatgctcga agtataattt
     1921 gattcgtcag caagcatcgc tacatcccaa acactgatca taaaatcttt taaacgttca
     1981 atcatttcag caatattgct aaaatcagct aatttttctt tagaataaaa aaaacattta
     2041 tatcagaatc aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat
     2101 ttatgtttat tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt
     2161 ccattacttc tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa
     2221 taaatcatta atttttagct aaattctatg agaagtttat ttttgttgtt tttgctaatt
     2281 aactaaaact agtacaaaaa atttaaaata gattattaaa cctattgctt attctattca
     2341 gtcatatata taagcgtcat cctttagtaa ctaacatccc acactacaat agtattataa
     2401 catatctatt caacttcaaa ttgttgaaat ttaagccatt tatatttact tttcctcaac
     2461 tttagaaatg attttactaa ccttatatcc gtaatacgcg taacaaacac attcattaat
     2521 aacaatcaac actaagctat attagctttt taaatgacta gggtaaataa aataaggatc
     2581 aaaaactttt aagtttctga ccctttcctt acttccgatt ggttaatagt gatcttttgc
     2641 agaataaata aatatcgttt cattttcaac tctatatatc agtctatgtt catctgtaat
     2701 tcttctggac cattttccag ataaatcatg ctttaatggc tcaggttttc ctaatccagc
     2761 aaagggggaa cgatcgatat cttttattaa cttgttaatc ttttttatat tgcttttgtt
     2821 tccttgctca tgccaataaa gataatcatc ccaagcatca tcagaccaag ccttaatcat
     2881 cagattcaac ctcgattaag tcatgtgttt taaatgcacc tttggagaat tgttcatctc
     2941 ctcgacgaat tttttccatg acgtaattat tagaaagtgt tctcaacgtt tcttgcatag
     3001 aatcataatc tctttttgat aatacaacaa ctgtatcttc tacatcttta cttgttacaa
     3061 taagtgtttc agcatcctca ttaacttgtt tcatataact acgtaaattt tggcggaaat
     3121 ttgaataagc tactgcttcc attcctttca ccccttattt atttctataa aacaattgta
     3181 cattatattg tacaattaag caaatgatta atattctaac ttcttataat taaaagtgct
     3241 atcactttta gtttttagga taaaaagtga cgtcactttt tgagtgctgg tacctcaaat
     3301 cctgcttttt cttgggcaat caataaatct aacgcaatac acataatcct ggattcactt
     3361 ttgccgtact tctgctttag ttgctgcaat tctttttgtc tcttttcatc caacgtcacg
     3421 ggtaaccgtt tatttcctat tgcaattggc attagtactc tctcctttca tcgaaataac
     3481 cctattaaaa atatggctgc aatcaacttg aatattagac tcacaagggt taacacaaag
     3541 cgccaaaaga tcaaacaaaa taccagtatt cctaagaata ctacgaagtt taaatgtaga
     3601 gctgcaccat gacgcataag tatctcgcca acaaacagta cgaacaatag aggaatgatt
     3661 ttattcatat ggatctcctt ctttctctta ggttatcggg ctttgcccgc ttatttcgca
     3721 ccacctaagc ggcgggggcg gttggatcga ttggtgcgaa ataagaacca ttcccttaat
     3781 ttcaaggttt tttgttctta tttcacacat cctcactttt cggtataaat ggttattgac
     3841 cttttttcct ttcaaatcgt tgatatggca ttcgtttacg gtcaagttca ttttggattt
     3901 cttctgtatc gtcgtaagtg taggtgtcta aaaaatcagt taaagtcgtg taccagtcgt
     3961 cgcctgggtt atctacttct tctacatatt ccctcaaaaa tgcttcgggt acagtgagtt
     4021 cataatccat tcccattaac acgatagatt ccgtcccaca cgtcaaaaat ctagtaaata
     4081 caaggtaata atcgtctacc gtcgcatctt tgctgtttag ctcttccatt tcttcttcca
     4141 ttaattccat gctctcaaaa attgaataat attctgattc tgagtaatag tgtttcctgc
     4201 aaatcgtgta gctgacaaag ttttcgacca gctcactcct ttctaggtaa acattcatga
     4261 tatatttttc tgtttctagt cgtagaagct ccgctccttt ttttaatgtg tcaggacgac
     4321 gtttatacac gtctaaacga ttatggctga ctgtaacctt cgtttgactc gtttcacttt
     4381 ccatatacaa ctgttcttcc actctttgaa taaagtactt catttcttcc atacaactaa
     4441 agtctttttg aatccgtctg attacttcat tcaagtgttc tttgtttgtt aaaaattgcg
     4501 aaacatcttc tgttgttagc tgataaacca ccttattcat tatctttctc ctccgattca
     4561 atcgtataaa atgcaccatt tttttcgata
//
LOCUS       DAJSVF010000146         4524 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSVF010000146 DAJSVF010000000
VERSION     DAJSVF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.155"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..238
                     /locus_tag="ON550_002759"
     CDS             <1..238
                     /locus_tag="ON550_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HCR3955781.1"
                     /translation="YDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKY
                     RHLWSETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            431..994
                     /locus_tag="ON550_002760"
     CDS             431..994
                     /locus_tag="ON550_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955782.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1273..1560)
                     /locus_tag="ON550_002761"
     CDS             complement(1273..1560)
                     /locus_tag="ON550_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3955783.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1550..1879)
                     /locus_tag="ON550_002762"
     CDS             complement(1550..1879)
                     /locus_tag="ON550_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3955784.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2184..2513
                     /locus_tag="ON550_002763"
     CDS             2184..2513
                     /locus_tag="ON550_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955785.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2800..3285)
                     /gene="vanZ"
                     /locus_tag="ON550_002764"
     CDS             complement(2800..3285)
                     /gene="vanZ"
                     /locus_tag="ON550_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCR3955786.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3438..4349)
                     /locus_tag="ON550_002765"
     CDS             complement(3438..4349)
                     /locus_tag="ON550_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HCR3955787.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1499 a          768 c          701 g         1556 t
ORIGIN      
        1 atacgattta aatccaagta agtgtttcgt agtaggcgat tccggtaaaa acgatatgat
       61 gttggccaaa aatgctggta taaacagtgt tttactttta acgggagagg gaattgatag
      121 tttaaccaaa tatagacatc tgtggagtga aacaaaacct acttttattg ctgaaaacag
      181 tttagatgct ataaataaaa tacatatgta tgcagatgga gactcttctg attcatgata
      241 ccagaagagt ctccatttat tactaaaaac cgtatcaaaa acaatcgatt atgagacaaa
      301 atgaaatgac ctcttttatt aaataaaaga ggtcatattt tgtatcataa cttgttgcat
      361 taactatgta tctataactc ccttttatga tacaatcaag ccagaattaa agtacgaaag
      421 gaagaaaagc atgagtggat acatttatgg ttatgcccga gtgagtacac gttcacaaga
      481 actaaatcgt caattagact tactcaatga acaaaattgt aatgaaatcc taactgagaa
      541 aatgacggga accaaagcgg ttcgtcccca actaaatcgt ttaaaagata aaattcgtcc
      601 gggagatacg gtaattgtag aaagtttttc tcgattgggt cgtagtacaa aggatttgat
      661 tgaccttgtt acctattttg aagaacacca tgttaagctg gtgagcttga aagaaaactt
      721 cgataccagc acgcctcacg gacgcttaat gatgactgtc ttccaagcat ttagtcagtt
      781 cgaacgtgat ttaattgttg aacgtaccaa agaaggtttg aaaagtgcac gtgcgagagg
      841 tcgtaaaggt ggccgtccac gagtgaacca gaaagatgtt gatcgagcag taaaattgta
      901 taaaagtcag gtttatagcg taaaagaaat tacggaaatg actggtatta gcaaggcaac
      961 actctaccgc tacttaaaag acaataggga ataatctatt ttaatcttta ggaatctata
     1021 atatttatgg gagaattaga gtgatttttt tattattact aaaaaacaat ataaaataac
     1081 gcattttaag ccctctttgt gcataaggat gaagctttta atttgaaaga ggcttaaaat
     1141 gcgttttaat tttttctatt tttcataaat atataggtta gtggtttaaa attatagggt
     1201 attacaactc tcagctgtta aaaattatct tttaaagtgt cgtctatcct ctcaagttga
     1261 aacattgggt tcttacctaa tcttttttaa ttgttcataa aaattttcat gcgttccaca
     1321 tagtaagata ggtatcactg cattatcttt aatttcatat gcaacgcgat atgtcgtacc
     1381 tgcatacttg aatccaatag cccaaattcc tgataaatca cccgtttttt gttctcccga
     1441 atgaggacga actgctatct catcataaat tatcgttaaa aattgttctt ttaatgtttt
     1501 ctcttttaat ttttttagat actttattac gtgtttagaa ggtagaatct cataagccaa
     1561 tttctgctgc caactcctct tttgtcatcg gtttagaatt tgcaacagta tcttcagcaa
     1621 tagatttaaa tgcagaagtt aacgcatttt ttctatttct aaattctttt aagatctctt
     1681 gtttgctata accttctgaa agaatatcag ccaaaatatc ttcactaaaa tcaaaaaatt
     1741 cttttttagg ctctacaaac tcataaagga taccattctc tacaagctta gcttctactt
     1801 caacaccatt gggcacatcg aattctttag ggaccgttaa cataatagaa tttccttgtt
     1861 ttcttgtttt tgtaatcata ttatttcctc cattatttta catttcgtaa ttacttagta
     1921 attacattgt acgctatttt tagtagaaat acaaaataat aaaactcctt tagaacaatt
     1981 ttcaaaggag ttttatttga ttaggaaatt atgtagaata ataatatgtt tttaacttgg
     2041 aatcaaaaac gcacttgcaa gtaagtttac ttcaatacat gaaattgctt ataatagtgt
     2101 attggaatta aggtagattg acaaaagagt gatcaaatag gtatacttca ttcaaataat
     2161 cgtttgaatg tgaggtgaga tgaatgaaaa aagtttgcca agtgactgtc gtacataaag
     2221 aaaaagtaaa tggaataaaa aataaattgg atcaaaaaga tttttcaaac ttacttgtgt
     2281 tagggaaatg tttcagcgat ttttctagaa ttaaaatttt ctatgcttta gaaacctata
     2341 aggaaatgtg cgtctgtgat ttagcagaaa tacttactgc tagtgtcgct acaacgtcac
     2401 accatttacg ttttttaaaa aaacatggaa tggcaaaatc acgtcaagat ggaaaagtag
     2461 tttattactc tttagcgaat gaagacatcc tttccgctat tcgtgttttt taaagttatc
     2521 agaaaatcta tctatgaaat cttagtttgg gggtaccgtc aggaaaatgc ggatttacaa
     2581 cgttaaggaa atttattaaa gagaccaatt cctcagcact aaaattgata attgggcttt
     2641 ctttgttact atagtgttag gtaagctttt tactttatca ccggttttcc cctcacttca
     2701 cacctacggg cgagtttccc cacatcccgt tttccatcta atccaattca ttcaatatct
     2761 ttatgtgtat gaaagatcag attgcttgat ttagacatct tacacgtaat ttattcttaa
     2821 atgggtacgg taaacgagca ataatacgag caaaagtata cctacaaaaa taataactct
     2881 gtctaatttt ttttgattca tatgcttatt gcttaaacca tataatttca gtccaagaaa
     2941 gcctccaaca gtatttgtaa ttacatctgt tatgtctgtc gctccaatag cgaagataaa
     3001 ttgaattatt tcaaaagtaa gacttaaaac cagtacaaaa gcaaacttag gtaaaaatcc
     3061 gatttcttta aaattgacat tcaaaagcaa gccaaatgga ataaagatta taacattatc
     3121 tatcatctct ctgaaattcc cagtagcagt aaatggagtc aagttaagac ttctttgatg
     3181 ataattaaat actgataaaa tattgtattg taatttgaat aacactaacc agattagtgt
     3241 cactaaatat aaagctagca atcctctaga taatattttt cccaatataa tttcctctaa
     3301 ttcttttgtt taagttgtat cattagtata gcaatgtcct agttaacgtg ggttttaaat
     3361 taaaaagcta acgttacccc aaaagtgcta gtacaataat gctgttcatt aatttcgttg
     3421 gtttcattcc gccatcctta cctccttgaa ttagtatgtg ttgatccggg aaacactgtc
     3481 acaattacac cgtctatatt gtttcctgat atctcataac gaagattagt cggcacatga
     3541 atggtggtat ttttagtaac aggataataa aagatctcat atttttcccc atttacacta
     3601 acagaaatgg ttttttcttc ttttaggtaa tccatatatt cctcgagaac gaaattcttt
     3661 tctttcataa tcgcactatg tggtaaacca acatagcgaa tatgccatgg ttcatattga
     3721 attcctgtta actctgtttt gtcctctgga taacgtaaaa tgaacccgta tttccaagca
     3781 ttttcttcta tccactttcc ttcaggggct cgttccattt tcgtcaagct tgatcctaca
     3841 tctagtgata aacctgaatt atgctcacta taacctgctg gtaaggcata ctcagccccc
     3901 atttcttggt aaagcacact ttgctcatca aagtctcgat agccactatt aataataaaa
     3961 tgactaacgc caccctttac agcatcattg accatctctg aaaatttttg tgctatttct
     4021 tttgacatat aaatattact atcaagcaac ccgtatccat ttattaattc gtcatgttta
     4081 gataaattca cgatatctga cttcacactt tcttggcgaa caggatattt actattgatt
     4141 aatagcagat ttccttgata aacctgttct tctgtaattg ttttctcttg ggtattttca
     4201 gaagtcccac tattttctaa atgttctttg ggattttgat cataattttg aaattcgact
     4261 ttttcctgag aaaacagtgc ttcattaacg tagtcataac ctaagtatat taagaataac
     4321 aataacaata aaaaaaacaa cttcttcatt ttcagtctcc tccttaacta tatagttgga
     4381 agtatgaaat atttctagaa tcttcccctt aaacggcaca atctcgtttg agattgtgcc
     4441 gttttgcccc gtaaattaca gtcaattatt tcgccctact ggatttacta ttatcaccaa
     4501 tgtagatgaa ttttcataag tgat
//
LOCUS       DAJSVF010000147         4337 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSVF010000147 DAJSVF010000000
VERSION     DAJSVF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.156"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(693..986)
                     /locus_tag="ON550_002766"
     CDS             complement(693..986)
                     /locus_tag="ON550_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR3955788.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1389..2567)
                     /locus_tag="ON550_002767"
     CDS             complement(1389..2567)
                     /locus_tag="ON550_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3955789.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2671..2985)
                     /locus_tag="ON550_002768"
     CDS             complement(2671..2985)
                     /locus_tag="ON550_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955790.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3092..3307
                     /locus_tag="ON550_002769"
     CDS             3092..3307
                     /locus_tag="ON550_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955791.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3607..3849)
                     /locus_tag="ON550_002770"
     CDS             complement(3607..3849)
                     /locus_tag="ON550_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955792.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3836..4264)
                     /locus_tag="ON550_002771"
     CDS             complement(3836..4264)
                     /locus_tag="ON550_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3955793.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1375 a          827 c          649 g         1486 t
ORIGIN      
        1 tatttttaaa taaaaaatac tcttttttta cgattattga aaaaaaaaga taaaacttga
       61 ttaattgaag tattgcttaa atttggaagc tatgagggaa gaataaaaga gttaggacgg
      121 gattatctac tatttttctt tgttttaaat atttgttatc tttgaacata aaagagaaat
      181 gtgtcgactg cagtgttgtg tccctcatca cgaataaacg gtgttcaact agctgatctt
      241 gtattcactt aaaaaatggt aaagtacgag ttgctattcc ccattttcaa ttttctaacg
      301 ctcaggacaa aatactgttt tcacaacctc attggaataa aagcatatat attgtttcaa
      361 gaacgaaatc ttgttatctt tttaaagggc atctgtccaa aatctaattt taggaggtac
      421 gttttggtat ttattcgtac aaataaaaga gaattatctt tattcatgta acgcatccaa
      481 ttgatttttt catgtacgct cctggaacga ttttactgac gattgcttat ttaactgcga
      541 atcgctcatt agcttttatc atagctatcc atattttgaa taacgttctt ggctttgtat
      601 tatagataca gatgacaaaa aaagttcaag tatgcggtgt agaaccaata tcgcatactt
      661 gaactttttt caatgagtat aaattaaaac ttctaatttt caattcccat catctgatat
      721 gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga ttagataacg tctttgttcg
      781 acataatcgc cactagaaaa tacgttcagc agtatcgaat taacctctgt ttctgtataa
      841 gtcactccat ggtcaaagcg tttcaagact tctttcaata ggatgtattt cttttttgtt
      901 gaacttggta cttgttttac ttttccatca ttcatgaatg tgtctaaaac tttcatcttt
      961 tcttgctccg tataagtata aaccatcttc tttctccttt tgattctaag aatactccat
     1021 ttatgttaga atattctgga ttttttatta aagcggttac tcttaagaaa gatattatca
     1081 tagtgctctt ttttttacta ctacttatag gtagatttat tctaatccat tattctcccc
     1141 taccaataat taaagaaaaa actggatcag ggtgttcaaa ttacatgcta agaaaaaaga
     1201 acccttgaat accaagggtt ctttctcagt ctgataacaa tgattaacga cggatttctt
     1261 tgatacttgt gaaaagtcgt taaagtattg atataaaagg atttttatta cctctgacta
     1321 catattgact aatttgttaa aaaatcgttt ttcaagaatc ctgtaatacc agtgctttca
     1381 ccgttttttt aactctctaa agaactaaaa gcaatcagag gttttacctt ttcatttgac
     1441 gttaagtggc gataaatttt cgaagttgtt tggtcagtat ggcctaagcg atctttcaag
     1501 acttcccagc tacagtgttc cgctactaag cttgccattg tatgacgagc taaatgagta
     1561 tgaaatggct tattaattcc tgagcgattc acgcaccgtt tgacgtgatc atttacatac
     1621 tctcgacgat aaggaacacc acgtttacta gaatcaagat acacaaacaa atatgtttct
     1681 tctataataa acctgtaatg ttgcctcatc tcacccaaag ccttaatcaa ctgttgtagc
     1741 ttttccataa ctagaggagt tactagaact cgtctagcag aactaactgt cttagtagtt
     1801 tcaattcgaa aatccttact cttaccttta actgccgttt gcttatagaa cagaatttca
     1861 tttcttttga aatctatatc tccaggctgt agagcaagcg cctcgctgac tcggcagcca
     1921 gtgtaaaata gaacatggaa aagtacagac atcggtaatt cctcatgaac aatgccataa
     1981 ttcaaaaaag ttctcaattc atcgacagta agaaacttgt cctcaatatt atttatggct
     2041 tcttttaaat cagttaccgt taactgatat tttggcattc tcacagactt cattggattt
     2101 tcacttatca aattctttct ttgacaatat ttaaaaatca tatttagaac ttgaataagt
     2161 gattgtaaac tatttttggc atagccaaca tttgttccat taacgccaca ttcctttaag
     2221 gtaaaaagca attcttgaag gcactctggt gtcaaatcag acaacttttt atcaccaatc
     2281 catcgaacaa taatttttaa ttgttcctct cgcttatcat aggttatttc cttttgttca
     2341 tttaacgaac ggtaatattg gaaccattgc tcacctacaa cactaaactt gtcattgctt
     2401 gcaacaattt ttttcttaag cctttgttgt ttctccattt ccagataagc gagttgcgcc
     2461 tcacgttttg aattaaagcc acgtcttgtt gttcgatatg cttgaaagta ccatgtgatc
     2521 ctaccagtag aactgatttg cttcttgaat cgatctgttt tttccattta ttgattcctc
     2581 ctattttgaa ggaacccaac aagcagaaac cctttggaaa tctttctatc tatgtcatca
     2641 ttgtaccgca tcttagaaag aaaatgtaac ttataaagat cgaagtagtc gcaatttttc
     2701 tcgatagtca ttcgaatcac tctttctcat ccattctaaa acatcatcac gaaaatagtg
     2761 tcctcgaatc ccgtaccaac agggaggaaa ctcaccaagt tttactaaac ggttcagtgt
     2821 actatcactt atctgaaata gtcgctttaa atcagtattt gttaatacca gaggtaaatg
     2881 actgactgca agcaattttt ccgtgtaatc ttccagtttt ttttgtaaaa ttgaatagaa
     2941 ctctatttcc agagaattaa catttgtttt aacttccaac tccatcaccc ttctctctca
     3001 aaatcaaaac gaccaccacg gtacccctta aaattgtaaa aaaacactcc agtactccat
     3061 caaaacggac tagaaaagag gaatttttaa tatgaaacaa ggaattgcag acataaaaat
     3121 aattaaagaa attcttgaaa aatctacggc taatgcaata gcattcggaa caggaatcaa
     3181 cttgagtaca gtaaaaaaac ttaaaagtgg agagagagct gaagaaaaat taaatcttgc
     3241 cgatgctatt aaaatcacag aatttggcat gaaaaatatg ccaaccaaaa tcgaaatatg
     3301 gaaatagtag tttttctgat ctatcagctg ttttgccaaa gcacatagga atctcacctc
     3361 cccccagtaa tggcgggccg atttggttac ggaagtatca ttatcgtagt cgcgattctc
     3421 atattaaaat ttgtcgctta aaagctaacc ttgtcatggc gtaactagag aatcaaaatc
     3481 tacatgtact gatagatttg ctattcattt gtcaaagagc gctaggctat tttcagccta
     3541 tggaaatcct ttattttgac ccgataaaaa acttccatag aatgaaaagg aaagggaaaa
     3601 aatactttaa cgagttgtat caaattcaag tatcttctca atcaattttg tttccagttg
     3661 tctacgcata tattcatcta catacataac cattcctttt tcttcttcat aaactggttt
     3721 catggctaac cgtgaaatat aattttgata ttttgacaga acgatggaca tggcaattga
     3781 atcaccagaa gctgcggctt tgattgtcgt tagtggtatg aatggtatat atcgtttatt
     3841 ttgcttcata tttgacaccc aaatactttc ctagctcttc caaagccatt tgtcgttggt
     3901 accaaacact gccgagactt ttcccaataa tatcgccaat ttcacgatca ttaagaccga
     3961 tgaagaagga cataaaaatg atttcttgtc tttgactagg taatgctgca atcgcatttg
     4021 ccaactcctc attggaaata aagattttca taccaaggac aataaaagta ccactactat
     4081 tcaaatttgg ttccaaataa tagaactctg tacctaaccc tttttgttcc aatatttcca
     4141 ttgatatttg ccgttggcga aatctagcgt acttcttttg aatatttaac gcctcattgc
     4201 agactacttt cttacaaaaa ctgtcaaacg tatgttgaat gtgggtttcc tctgagtcgc
     4261 ccataatatt ctccccctca aaaaattcat tcaactcgtt ttacttctat ccctttccat
     4321 tattactaca atctagg
//
LOCUS       DAJSVF010000148         4309 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSVF010000148 DAJSVF010000000
VERSION     DAJSVF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.157"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..999)
                     /locus_tag="ON550_002772"
     CDS             complement(<1..999)
                     /locus_tag="ON550_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955794.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV"
     gene            complement(1023..3314)
                     /locus_tag="ON550_002773"
     CDS             complement(1023..3314)
                     /locus_tag="ON550_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3955795.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            complement(3324..4061)
                     /locus_tag="ON550_002774"
     CDS             complement(3324..4061)
                     /locus_tag="ON550_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3955796.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            complement(4112..>4309)
                     /locus_tag="ON550_002775"
     CDS             complement(4112..>4309)
                     /locus_tag="ON550_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955797.1"
                     /translation="LQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQ
                     TTQQIIDSITKVQNDNASAWR"
BASE COUNT         1289 a          903 c          637 g         1480 t
ORIGIN      
        1 aacaaagcta atactcatat aaaatcgatc taaatttgtg atttgataat taacgttagc
       61 tgtacctttg attgttattt ctttccctac atcatctgtt gtaatatttg tactatttgc
      121 ttccaataat atcttttctc ctggtgatgt acgatacacc aaacgtaatt tatcaatagc
      181 tcctgtagtt ccttcatcaa atcgaacttt cgcactcaga gtataggttt tcccactagt
      241 aagctgaggt gtattaatac gcgtaaacat aataatgctt cctgtaccat cagaagtaaa
      301 atgtaacttt tcgttatcca aagttagtga cccgtggtaa ccaacgttaa aatcgctcga
      361 ttttagtttg gacattaaat tcggcttacc gctataatca tatcccccga agtcgatgct
      421 gttactgtac atcacttgta agttacctaa cttagaaatt tcttctttca gagcatctaa
      481 cttgtcttgt agcgttttag cttgaccagt taaatcagta atctgttgat ttaagctatc
      541 cactctacct ttgatttcag ccataaaagc atcaaaagtt tcgttgtact ttcgaatcaa
      601 ctcttctaat tgcgaaacat attcatcggc ttggccttgc gaaatgtcag acactcctag
      661 tgagaaaaaa atgatatctt gcgttgttaa aatttgattg tcttttctat attctacgta
      721 gcagtgttta taatatcctg cttcactcat aaatgtgcca tcaagagaaa acgtgacttc
      781 ttcactagtt acactagttg caacattatc tacgtaacgg ttagatggtg ttgttccttt
      841 taaagtaaat gttccgccac tcgtatccat ctgcaagcca tttagaaacg gtttaaccgt
      901 caccgtaatc cctttatcac cttgacgagc cataatagct ttggtgtagt ttaattcttt
      961 gctgaaatct aaagccaaat tatataaact gctagccatt tatatacctc cttgtcttcg
     1021 ttttaaaaac gtttttggtc aagcactgtg ctatcatatg ctgtatcctc ttttaatcta
     1081 atatcttcat accctagacg gtgtgccact aaattccatc taactaatac gtttggctta
     1141 ctagtttcaa tgatgaaatg gtcaatatct tcatgagtaa cagcacacaa aactagttct
     1201 gtaggtgtca catgtgtcat ataccgactt agatttactg tttcagcaaa catggggtca
     1261 atatcaacac gaactttacc atcgtcacct gtaacggctt ccccataatc agcgaaataa
     1321 tattctggag tttcataagc gttcaatagt cgttgtccat aatgttctgt tggtacagtt
     1381 gagtttttag tacctctaac agtaaaatct ttatatactt gtaccgttga ttgttcaaac
     1441 ctagcaagtt tcccatcttc ccatgaacca aaaaaacaac ctggtaacgt tagcatacca
     1501 tcactagtaa atttcatagt cctaccagct accttaaatt cccatgagtt acccgcacta
     1561 ccattaatgc ttaaagaact accgtcgcca gaagttacat aactagcatt gctatacctg
     1621 aaattgggcg caccaaaaga tagaaacggt ctgttattac cattatccca cgtactaaaa
     1681 accaagttac cctgtggatt tctaatcatg aaaccaccac cagttttcat ggtgtatgat
     1741 acaataccgg catcagcact tacataatca cgtgcttcta gctccataat atctttgtta
     1801 actttttttg agtaccaagt cattttgcca ttagcaatac ttgttctata atcagcacca
     1861 tcactaatta atgtagtacc tctaatagta attcctacta tttcaccagc cgtaataaac
     1921 gaggcattga atccgccatc taacgtccat gccgtttcat atgttccatt aatgccagtt
     1981 ttagaaaaac caataccagc attgttgatt tgtaaaacat tccttgcggt attcttatct
     2041 ggtgtgtcca taatcaaaat acgactaggc gcttctttag gatctaataa aacataacca
     2101 ccattttgac cagtaatcat atcagtttga tgatctacaa tatcattgag taaatcactg
     2161 atttcgccac cgtttttcaa ttgatcaatg gcatcattaa tcaaattgct gacattattc
     2221 tctgtgtttt ctaagaagtt tgttttgacg tttcctacaa ctaatttatc gtatgaattg
     2281 gttagaacat taaacgtata ttccacaatt ctcgctgaca tattcacttt taactgtgga
     2341 tgatacacat ctactccgtc acccatcgaa actttttcta gatcaacaaa tttttcatag
     2401 cctctttgat gcctcaatgg tactaattca atcgaaccac tcacttgtgg tttttgttta
     2461 tctatgtttg ttttcaacca gtctttagca gcttccctta atgtggctac atcagtcgct
     2521 ttgtctttaa aatcaacaaa agaaacatat ccagcaggat aatcatccac gtaatccgtg
     2581 aaaataactt cttctggtag agtgatctcg tcttctcctt ctgaagagct gctaatgaat
     2641 ggataaactc caactaaaac actttgagca tcaatctcta agtcaagacc agttaagttt
     2701 ttagtataaa tcgctttgat tttatgatcc gtgcctagcc ttttttcatg acgtaatgtg
     2761 ttattatctt ttagaaattc cccatgaaat cgatctagaa tagatccctc ttttccacca
     2821 aagaattcta aaaaattcgc cttttctatc ttcacattag caagcgtatc tactaatgac
     2881 gagaaagaaa actgtgaagg aataactggt ttcgctaaag tttttgcgtt ttgccatgcc
     2941 tgactagcag tgatcttttc tgttccactg tcatatttat tcaacaccga ttttcttata
     3001 tcattgaaaa taggttcagc ttttacttct atcgtattgc ctattacaga agtctttgca
     3061 taataaatcc gtagacgctg ttttgctcga ttttcatcta cataacactg aataatacgt
     3121 ccttctacaa tcaaatctgc attagttccg cttattggat aagtaccctg aaatatctcg
     3181 gctccgttta atttattgct aacagtagct gttaaccagt ctgacaaagc gcctaaacct
     3241 tgcgtatcat ataaatgttc agctaaatta ttcgcgtcgt ttttatcgta aatagttatt
     3301 aaattatcga tcatctattt cacctacctt aacccattac gataaatttg tattttgctc
     3361 aaaccagtac aattaaaata attaatatcc acttgcaatg tcggatattg catggtcttc
     3421 attttgttgg accgatctaa aatatctccg tccgattgct cttcgtagca aagcatcaaa
     3481 tcactatcaa tgactacgtc agttcctact actaagcctt caaaactaaa cacataatca
     3541 tttaagatga actggcatga agtagctgaa ggagtgatga taatctttgg aaaactttct
     3601 tctaaactat tattcagcaa gttaaatgac tgtggtttat ctacggttat aggtacatct
     3661 tcttgaactc ttgcgaatgg tttcgcagta atatttacat cgaactctcc ccattcaaca
     3721 atatcgtttt ctgcatcccc aatatcgata gtctggataa cataatagac gttgggatca
     3781 tcagagaatt ctaatttctt tgcataattt aaccaatgac gcatgatata aaacgattgc
     3841 ttgaacgctt gatggtcttc cacatcctct aaatagttat agtgcaatgt aaacgacatg
     3901 tcttcaaacg agtaatcttg tactaagcca cctaaccttc ctaaaacaga agtttcaact
     3961 ctctgtcttt ttggagaagg tatggttggt ctttcagcta aagccaattt atgcaaataa
     4021 tcaggaaatc catcgattat agaatgtata caatcagtca ttttttcaca tcctttttaa
     4081 tactaaaaaa acaggagaaa tactctcctg tttaacgcca tgccgaagca ttatcatttt
     4141 gaacttttgt aatgctatca atgatttgtt gagttgtttg cttcatagta acctcatctg
     4201 cgttaccatc aattgtgaaa ttgaattcgt agttattcac aggttgaatc gtttgtgccc
     4261 tagatgaaac tgatgtgcta ctcaagatac gatcaccaat ttcttgcag
//
LOCUS       DAJSVF010000149         4285 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSVF010000149 DAJSVF010000000
VERSION     DAJSVF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.158"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..99)
                     /locus_tag="ON550_002776"
     CDS             complement(<1..99)
                     /locus_tag="ON550_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3955798.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQE"
     gene            complement(297..1724)
                     /locus_tag="ON550_002777"
     CDS             complement(297..1724)
                     /locus_tag="ON550_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HCR3955799.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(1869..2417)
                     /locus_tag="ON550_002778"
     CDS             complement(1869..2417)
                     /locus_tag="ON550_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955800.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2418..3275)
                     /locus_tag="ON550_002779"
     CDS             complement(2418..3275)
                     /locus_tag="ON550_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3955801.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3561..3848)
                     /locus_tag="ON550_002780"
     CDS             complement(3561..3848)
                     /locus_tag="ON550_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3955802.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSVIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHEKFYEQLKKLI"
     gene            complement(3838..4167)
                     /locus_tag="ON550_002781"
     CDS             complement(3838..4167)
                     /locus_tag="ON550_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3955803.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         1284 a          820 c          632 g         1549 t
ORIGIN      
        1 ttcctgaact tcacgatagc ttagattgta acgcaggtag taaccaacag cgacaataat
       61 gacgtctttt ttgaattgtt tgcctttaaa atgattcatt actctgtcct ctctgtcttt
      121 tttctcaatt ttacactaaa atagattttt tggaaaactt tgcaacagaa ccaccgttcc
      181 tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc ctctatttca
      241 cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag ttcttctcag
      301 actgtagaca aaaaggagtg gcttaattgc caactccttt ttgttagtct ttgtagaaat
      361 tttaaataaa aagccatagg cttttttgct ttccatagcc atgtggctag tttttttaaa
      421 ttcatggcag caaacgtaag catcgtgtgc gtggcgactt tttcgagacc tcgatatttc
      481 gtccaacgca taccatgctt ttcttttgca tctgcaaata ttcgttcaat ggtttgtttt
      541 ctctttttat aaatttctct attcaacgcc gtatggcgtt ggtgttcaag ttcatccata
      601 agatccgccc aaacatgtcg agttactatt ttttgatgtt ttgtactcaa agtacagttt
      661 ttaagtaatg gacaactcct acagaatttt ggatttgatt tataggttcg ataaccatca
      721 cgagtgatag ttgaaaataa caacggttga ttattgggac ataaatagca gtcaaaatag
      781 gcatcataaa caaagtcttt tttacgaaac attcctttta ctccatgagg tacagtatat
      841 ggaagaatcg ggtagagttt caagtgtgac agaaaatgaa caaatttagg cgtcttatat
      901 cctgcatcag cgacaacacg ctttatattt ggaaaagctc ttttcgactg tttgaccaga
      961 tcgattgcga cttggctgtc atgtacattt cctggtgtga ttaatgtaga taagataaaa
     1021 ccattatctt cacaggaagt atgcgcggaa taagcaaatt gtttttctcg ttcaccttta
     1081 acatagtaac cactttctgg atctgaatga cttatttttt gaagactcaa ctcactttct
     1141 gtgtcccatg taaaaggacg ttttcctaca gcaatacgtt ctttatttat ttcatcttct
     1201 aattcatctt gataagcttt agcttgaccg taggtcattt cttttgtgaa cttatgttta
     1261 ttggcatttg ccttaatatg agtagaatca atataaaggt tgtcttccgt aacgaagcca
     1321 gcttttacag cttgttctag aatatgagag aaaatgtctt caaataaagt tgtttctctg
     1381 aatcttcgaa catagttttt accaaaagta gagaaatgcg ggataggatc ctcgaatgaa
     1441 taacctaaaa accaacgata agcaacattt gtttcaacgt ctttaatagt ctgtctcatc
     1501 gaacggatac caaataaata ttggataaat actaatttaa ttaagaccac tggatcaata
     1561 ctgggacgac catgggtcga atacgtagat tctacaatgg gataaatgaa atcaaactga
     1621 atcgcagcat caatctttcg aactaagtga tctttaggta caagatcatc aattgttaac
     1681 atagcaaatt gatttctacc ttcaatcgat tgttttctca tcattagaat cactcccaga
     1741 atagtttata caatgagtat aaaacaaaaa aactgcaaac aacactccca atttaggagt
     1801 ttgtctacag tctgagaaga actaagtaac taccgtttaa aggtagttac ttagttcttc
     1861 tttgattatt atctaacttt attattcatt ccccaccctt tgcgcagtga gtcttcggta
     1921 tcttgtttta tctgactggt agttcttatt atttctccac gatcatctaa ttctggaaca
     1981 attggatatt tttctctgaa caagtctagt tctttcacca cgtcattaaa caaatcttgc
     2041 ttatcttctt gcgtaagatt ctgtttaata tactcatcta aagcttttct aagcaattca
     2101 tctcttgtca tattagtctc atacttttct actaaaactc tggaagtcgc atttaacaaa
     2161 gtcgacagtt ttaaaggatg cttggtggaa taaaccttat caaactttct gattggtgcg
     2221 ccaacttttt ttgttcttcc tctactctgt ctttcaacat tggaggaagt ttttatttct
     2281 tgaacttggt gcacttctaa ttgcttattt tcagatggtg taattgcttg ctgctttttc
     2341 tttgcttctg cttctttttt tattttggcg agtcctttat tgttcccaaa aatatcaagt
     2401 cctttgatgt cagccaatta ctccacctcc gcataccaat tctcgtgttc ttccaattct
     2461 ttcaaaatac tgataaataa ttcgtggctt ttgcgatccc attgttttaa gagacctttc
     2521 tttgtcttct ccatcgtaat acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt
     2581 acaacatttt taatcaaatg ttccccataa atctcttggg cttgtttata catttcctga
     2641 tctactgcgt caccgctttc caacatgaaa ggaataacac cagcaacata taaatttgta
     2701 tcaaattctt tcacaaaaaa gtccatatac tcaagatatt gcatcgcatt tctcatcgat
     2761 tttacttggg tctgtaagac gacgattgcc cagtccgcaa tgtacatcgc ggatttacta
     2821 tatacagaaa tagaaggtgg gacatctaaa tagatgacgt caaactcatc aaataatggt
     2881 tctagctttt ctttcatata tttgaattgg gataatgaat cattatattg gtctttgatc
     2941 caatcatcat acatttcaaa ggacatgtct gctggaagta tatagaggtt attgattact
     3001 ttatagacag cctctttagg tggaagattg tttatcaaca cgtcagtcac agtattttcg
     3061 taaacaatcg gttcaccttc tccgcctcct tcttcgttag tgaagttcat atgcgttaag
     3121 ttcatcacat cagaagtatt tccttgcata tccaagtcaa ttatcaaggt tctttttcct
     3181 tcattaactg ctgattcaaa ccctaacatt tgcgcgcacg tacttttacc tacgccacct
     3241 ttaaaattac cgattacata tttcttcgct accacgttcc tacctctttt cttttagcaa
     3301 tattcatttg atactattat aacatattta tttttagtta ctaccaaaaa aaagatatat
     3361 atgtactacc ttataattaa taaggaaaag gtagtacata aataaggaaa agctgaaata
     3421 tattgattta tagctttcct caaagtaatg acgttattta atttatcgag aagcagagaa
     3481 tactcgcgac tttagtcatg tagacgaatc agattttcga taaaaaagaa gccgtccttc
     3541 ttaagaacga caacaaatca ctagattaat tttttcaact gttcataaaa tttttcatgc
     3601 gttccacata acaatactgg aataaccttg ttttctataa tctcgtaagc tactctatat
     3661 gttgtgcccg catatttaaa cccactagac caaataactg ataaatcccc agttttttgt
     3721 tcacccttat atggatcaat cgcaatttca tcataaatta aattcaaaaa tttttctttc
     3781 aatggcttct cttttagctt ttttaaatac ttaatgacat gttttgaagg aacgatttca
     3841 taagccaatt tctgttgcca actcctcttt agtcattact ttagaattag taagtgtatc
     3901 ttcagcaata tctctaaagg aagaatgcat tttgtttttt ctattcttaa attctacaag
     3961 aatctcatct ttatcgtagc cttctgcaat tatatcagac aaaatatctt cgctaaaatc
     4021 ataaaattct ttttgtggtt caacaaattc ataaagaata ccattctcta ccaactttgc
     4081 ttcaacttcg actccattag gaacattaaa atctttaggt actgtcaaca tgattgaatt
     4141 gccttgtttt cttgtttttg taatcattta aatccctcat ttctaacagt atgtaattac
     4201 ttagtaaata cattatacca cgagataatg tatttacaag taattactgt gactcgtaat
     4261 gcgtccttgc tgaataaatt tcaac
//
LOCUS       DAJSVF010000150         4235 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSVF010000150 DAJSVF010000000
VERSION     DAJSVF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.159"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(37..1257)
                     /locus_tag="ON550_002782"
     CDS             complement(37..1257)
                     /locus_tag="ON550_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR3955804.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ON550_002783"
     CDS             complement(1277..1888)
                     /locus_tag="ON550_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3955805.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ON550_002784"
     CDS             complement(1931..2863)
                     /locus_tag="ON550_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3955806.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ON550_002785"
     CDS             complement(3284..4144)
                     /locus_tag="ON550_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955807.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1203 a          855 c          694 g         1483 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acccgtaccg catctgttgc cccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa gatat
//
LOCUS       DAJSVF010000151         4197 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSVF010000151 DAJSVF010000000
VERSION     DAJSVF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.160"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(61..831)
                     /locus_tag="ON550_002786"
     CDS             complement(61..831)
                     /locus_tag="ON550_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR3955808.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
     gene            complement(1315..1476)
                     /locus_tag="ON550_002787"
     CDS             complement(1315..1476)
                     /locus_tag="ON550_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955809.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            complement(1473..2144)
                     /locus_tag="ON550_002788"
     CDS             complement(1473..2144)
                     /locus_tag="ON550_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3955810.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            2667..3257
                     /locus_tag="ON550_002789"
     CDS             2667..3257
                     /locus_tag="ON550_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR3955811.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3447..4160
                     /locus_tag="ON550_002790"
     CDS             3447..4160
                     /locus_tag="ON550_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955812.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1354 a          729 c          618 g         1496 t
ORIGIN      
        1 attaagtcga gcacaacgac gttttgaagg aactttgaga tattctttag gatattaaaa
       61 ttaactagca ccacgggtac gtttatatat ttttgttttt gagaagcaaa actcctcttc
      121 gttaacatat tttgcagcca tttttttatt ataattacta acttttcttg cttcttcctc
      181 taatttagca tcagataaat tacgaatatg tctagtttga ttttcttcct ggttatatat
      241 atctttatct tcaatcaatt gctcttgatt tattaaagct aatggaaatt tatatacttt
      301 tctgctgttg cccgaactat ctttttcatt aacagtatat atttgtcctg ctaaatatac
      361 ttctcccaga tacgtgtgtt cttttggttt aaatgtttca aataaatgaa tctttacaaa
      421 attacttttt gaattggcta aaactttatt ttgattctta tccaatactt gatccccttt
      481 tagccccata ccggtatatt gtaaaatacc atttaaccac ttatctttat aaacattact
      541 tcctgtttga tcactaaaca gtacaagagt attagtttga tgagatcttc tcattccacc
      601 ctgtttgaaa catttaaact tctctgaaat ttctttgtta gtatatactc taccaacttc
      661 taactcacct aatatgtttt tagaccaatt ttctcgccat gtaataatct tatcaagatt
      721 aaacatttct ccctcttttg gcatgccatt tctaattaaa tcagctaata caccagtaga
      781 aatatctaat ttttttagta attctttctt agtaatcaat gttggattca taacaatgca
      841 gctcctaatg atatcaatat aattgcatta gataacttga aaaatcgaaa aaaactcatt
      901 ttgtgtcaag ttatctgtaa tatataaaaa catcattatt atacctcatc tatgtgaatt
      961 tttactattc ttaccttagc atattcttta taggtatatc agagctaatc aaactaaaaa
     1021 ataccccaag tcttcttatg taaaaacacc ggttattact taaatatgac ttaggtaaaa
     1081 caatgagtga tccaattata aaattttatg gcgaataatt tagtaagcgc tacatattga
     1141 atgatttaga aattttgaaa aagtagctag ttccttttat accagaaaag taaacctaat
     1201 aaaaaacaca actaattttt agctgtgttt tttaagttac aataattatc tatttaatag
     1261 tcttaattca ttaaaacaaa aaactcaact ttacaaggat atggtttaaa aaatttattt
     1321 atccaaccca actagttttc gctgcttacg atttgcttca tctgctacgt acttcattac
     1381 tgttttaaat tctttcgctg aggcgttttc tgataaatca gcgatacttt tattatactt
     1441 cttcacgatt tgttgtactt gttctttctc agtcatgtaa ttcctcctcc aactcttttg
     1501 aagtatattg tattgggtag tactaattga atattatctg ctgcataatg cacttcagaa
     1561 atgtcgtttt gataatcctt aatcattatg tgaaggacta cttcaggatg tctatttttg
     1621 actttttcaa tattctctct ggacttaaaa aacgcattga taaacatttc ttttggcaca
     1681 tgacgtcctt cagctagttc acgttttcga gtgaaatccc aggcaacttt gggatcttgg
     1741 taaacataga aaatcgtaac gcgaaagtta tttttaaggg ctcgaataat atttttctct
     1801 gcactaagga tagcaaaagt atcctctagt atgaaagaat agccatgttc cagtacatat
     1861 tttaatgatt gttcaactaa ccaagaagaa gctttctgaa aaacacttga gttctttcca
     1921 ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa tcacaacatg gttgtcataa
     1981 ttttcagcta aacctagtgc aacctctgtt ttacctgctc ctgggcttcc agccatgaat
     2041 accgcatctt tttcatcatc agggattttt cccttgatga ttgtttcaat aaatttatct
     2101 ttattgaccc tagcgtaatc taaatacttg tctttaggtt ccaagattgc accttctttc
     2161 cttcttctat attcatacta attatacgat atttatataa aaaatataac ggtcatttta
     2221 accatattaa catttagtca cagtaatagc agatatgctc gcaaaatgag atatgaatgt
     2281 acgcagaatc cttgttttct acggcattcg ctgttttttc attggactag ttatatattt
     2341 ctagggttgt tttcatgtct gagtgcccga tatgatttat ttttcgtttc tataattact
     2401 ttttgccttc cagtttcttt taagattgta ctcaagcaag tgggaattgg ttcttttgtc
     2461 atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa tgattacctg tcatttttat
     2521 ttagttcgta tttaattaag ttgactgtct gtatactctg tctaaaactt aagaattgtt
     2581 gttttcgctg tcgctatcca aatttaatga atgtctgtat gttaaaactg ttgtccttgt
     2641 catagatagt aaaggagagc ttgaaaatga aaactgcgat tacgtttcta ttagaggaat
     2701 atgccgattg ggaaggtgct tatattacta gcacactaaa ccaaaaagag ggatggcaaa
     2761 acaaaactgc ttctatccaa tcagaggtca cttccattgg aggattaaaa acaaaaatcg
     2821 attacttaat cgaacaaata cctgaaaaag ttgacctttt tattatgatc ggtggcaatt
     2881 catgggacat aaaaaatcat catttatatc aaataatcga taaattgtta aaaacagacg
     2941 taccagtagc tgctatttgt ggggcagttg attatttagc tcagagcgga ttattgcaag
     3001 gttacaaaca caccggaaat gcgcaatttc cttggttaga atcggaaaat tatcagaata
     3061 aagaagattt tatagaacaa caagctgtga aagatcgtaa tttgattact gcgaatggaa
     3121 cagctcctat agaattcaca gacttgattc ttcaagcagt ccattttgaa acagaagaaa
     3181 taagagaaaa aacgattcgt ctatacaaac ttggattcta tgattactat aaaaagtacg
     3241 gagatccttt ctcctaatca ttctcttcaa ttaaatactc cttctcaata gtatgacaag
     3301 taattttact gatggttatt tgtcacactt tttctgacat ttatcaaaga agatatactc
     3361 tatttatatt catagacaag gttttacgga ttcaaaaatt caggattacg ctatactggt
     3421 attaaaaaaa taatgaggta ttttatatga aaaagttagg ttttatttta ttgttggtct
     3481 ctttattcct tgtaggatgt acgtctccta gtaaagaaaa tagtgatcca tctgcgacca
     3541 cagacaaaag ttcaatcaga caaaccagtt cagctgaaaa atcaagtact cagaaatcag
     3601 cttctactga aacgaataca tcttcaacaa gcagtaaagt ctcagagccc aatacttctt
     3661 ctacatctag tagtacagtt gagacaaata tttcttcttc aacaatagac acaacttcct
     3721 cacagtcttc tcagtcaata gatccgttag caggatattc tgatctacaa attgagtacg
     3781 cacgtgtttg gctagctatt agaggtaata cttttagaga tacatcgagt gatttcgaac
     3841 tacatgttat tcattatcct gcaggaacac ctatcgcccc ttatgatgca gggagtgcag
     3901 tctatccgaa agatgtggtt atgctcactg ggaaatacag ttaccaggga gttttagtgt
     3961 attcaagtaa ccatgacggg acaatcacta gttatccagt tcctacacat tggcaaatgc
     4021 ctgcagatca aacatcttat ccagcgttca ttagagaaac aactcaagaa attattgata
     4081 atgcatctgt agtagctatt cctacaggta atccaaatga tgtgaaacag ctgatcaatg
     4141 tgatagttat ggataattaa aatttctaat agatcataca aaaagaagct ggcaaaa
//
LOCUS       DAJSVF010000152         4067 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSVF010000152 DAJSVF010000000
VERSION     DAJSVF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.161"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..981)
                     /locus_tag="ON550_002791"
     CDS             complement(<1..981)
                     /locus_tag="ON550_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955813.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLFVNKLESSDF
                     YMGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     NKLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRF"
     gene            complement(1005..3296)
                     /locus_tag="ON550_002792"
     CDS             complement(1005..3296)
                     /locus_tag="ON550_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3955814.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNMIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVNNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKIA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            complement(3306..4043)
                     /locus_tag="ON550_002793"
     CDS             complement(3306..4043)
                     /locus_tag="ON550_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3955815.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDAEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFSLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
BASE COUNT         1223 a          859 c          588 g         1397 t
ORIGIN      
        1 aaaacgatct aaatttgtga tttgataatt aacgttagct gtgcctttga ttgttatttc
       61 tttccctaca tcatctattg taatagttgt attatttgct tccaataata tattttctcc
      121 tggtgatgta cgatatacca aacgtaattt attaatagct cctgtagttc cttcatcaaa
      181 tcgaactttt gcactcagag tatatgtttt gccactagca agctgaggtg tattaatacg
      241 cgtaaacata tcaatgtccc ctgtaccatc agacataaaa tgtaacattc cgttaacatt
      301 agttagtgac ccatggtaac ccatataaaa atcgctagat tccaatttat ttacaaataa
      361 gttgggattc ccactataat catagtcacc gaagtcgatg ctgttactgt acatcacttg
      421 taagttacct aacttagaaa tttcttcttt cagagcatct aacttgtctt gtagtgtttt
      481 agcttgacca gttaaatcag taatctgttg atttaagcta tccactctac ctttaatttc
      541 agccataaaa gcatcaaaag tttcgttata ctttcgaatc aactcttcca attgcgaaac
      601 gtattcatca gcttggcctt gcgaaatgtc agacactcct agtgagaaaa aaatgatgtc
      661 ttgcgttgtt aaaatttgat taccttttct atattctacg tagcagtgtt tataatatcc
      721 tgcttcactc ataaatgtgc catcaagaga aaatgtgact tcttcactag ttatactagt
      781 tgcaacacta tctacataac ggttagatgg cgttgttcct tttaaagtaa atgttccgcc
      841 actcgtatcc atctgcaagc catttaaaaa tggtttaaca gtcaccgtaa ttcctttgtc
      901 accttgacga gccataatag ctttggtgta gtttaattct ttactgaaat ctagagccaa
      961 attatataaa ctgctagcca tttatatacc tccttgtctt cgttttaaaa acgtttttgg
     1021 tcaagcactg tgctatcata tgctgtatcc tcttttaatc taacatcttc ataccctagt
     1081 cgatgtgcca ctaaattcca tctaaccaat acgtttggct tactagtttc aatgatgaaa
     1141 tggtcaacat cttcatgagt aacagcacac aaaactagtt ctgtaggtgt cacatgtgtc
     1201 atataccgac ttagatttac tgtctcagca aacatgggat caatatcaac acgaacttta
     1261 ccatcatcac ctgtaacggc ttctccataa tcagcgaaat aatattctgg agtttcataa
     1321 gcgttcaata gtcgttgtcc ataatgttct gtcggtacgg ttgagttttt agtacctcta
     1381 acagtaaaat ttttatatac ttgtaccgtt gattgttcaa accttgcaat tttcccatct
     1441 tcccatgaac caaaaaaaca acctggcaac gttagcatac catcactagt aaatttcata
     1501 gtcctgccag ctactttaaa ctcccatgaa ctacccgcac taccattaat gcttaaagaa
     1561 ctaccgtcgc catcagttat ataactagca ttgctatacc tgaaattggg cgcaccaaaa
     1621 gatagaaacg gtttgttatt gccattatcc cacgtactaa aaaccaaatt cccctgtgga
     1681 tttctaatca tgaaaccacc accagttttc atggtgtatg atacaatacc ggcatcagca
     1741 cttacataat cacgtgcttc tagctccata atatctttgt taactttttt tgagtaccaa
     1801 gtcattttgc cattggcaat acttgttcta taatcagtac catcactaat taatgtagta
     1861 cctctaatag taatccctac tatctcacca gctgtaataa acgaggcatt aaatccgcca
     1921 tctaacgtcc atgcggtgtc atacgttcca ttaataccag ttttagagaa accaatacca
     1981 gcattgttga tttgtaaaac attccttgcg gtattcttat ctggcgtgtc cataatcaaa
     2041 atacgactag gtgcttcttt aggatctagt aaaacatacc caccatcttg accagtaatc
     2101 atgtcagttt gatgatctac aatatcattg attaaatcac tgatttcgcc accatttttc
     2161 aattgatcaa tggcatcatt aatcaaattg ttgacattat tctctgtatt ttctaagaag
     2221 tttgttttga cgtttcctac aactaattta tcgtatgaat tggttaaaac attaaacgta
     2281 tattccacaa ttcttgctga catattcact tttaactgtg gatgatacac atctactccg
     2341 tcacccatcg aaactttttc tagatcaaca aatttttcat agcctctttg atgcctcaat
     2401 ggtactaatt caatcgaacc actcacttgt ggtttctgtt tatctatgtt tgttttcaac
     2461 cagtctttag cagcttccct taatgtggcc acatcagtcg ctttgtcttt aaaatcaaca
     2521 aaagaaacat atccagcagg ataatcttcc acgtaatccg tgaaaatgac ttcttctggt
     2581 agagtgatct catcctctcc ttctgacgaa ctgctaatga atggataaac tccaactaaa
     2641 acactttgag catctatctc taagtcgaga ccagttaagt ttttagtata aatcgctttg
     2701 attttatgat ccgtacctag ccttgtttca tgacgtaatg tgttattatc tttaagaaat
     2761 tccccatgaa atcgatctag gatagatcct tcctttccgc caaagaattc taaaaaattc
     2821 gccttttcta tcttcacatt agcaagcgta tctactaacg atgagaaaga aaactgcgaa
     2881 ggaatagctg gttttgctaa aacttttgcg ttttgccatg cctgagtagc agtgatcttt
     2941 tctgttccgc tgtcatattt attcaacacc gattttctta tatcattgaa aataggttca
     3001 gcttttactt ctatcatatt tcctattaca gaagtctttg cataataaat ccgtaaacgt
     3061 tgctttgctc gattttcatc cacatagcac tgaataatac gtccttctat aatcaagtct
     3121 gcattagttc cgcttattgg ataagtaccc tgaaatatct cggctccgtt taatttattg
     3181 ctaacagtgg ctgttaacca gtctgacaaa gcgcctaaac cttgtgtatc atataaatgt
     3241 tcagctaaat tattcgcgtc atttttatcg taaatagtta ttaaattatc gatcatctat
     3301 ttcacctacc ttaacccatt acgataaatt tgtattttgc tcaaaccagt acaattaaaa
     3361 taattaatat ccacttgcaa tgtcggatat tgcatggtct tcattttgtt ggatcgatct
     3421 aaaatatctc cgtccgattg ctcttcgtag caaagcatta aatcactatc aatgactaca
     3481 tcagttccta ctactaagcc ttcaaaacta aacacataat catttaagat gaactggcat
     3541 gaagtagctg aaggagtgat gataatcttt ggaaaacttt cttctaaact attattcagc
     3601 aaactaaatg actgtggttt atctacggtt ataggcacat cttcttgaac tcttgcgaat
     3661 ggtttcgcag taatatttac atcgaactct ccccattcaa caatatcgtt ttctgcatcc
     3721 ccaatatcga tagtctggat aacataatag acgttgggat catcagagaa ttctaatttc
     3781 tttgcatagt ttaaccaatg acgcatgata taaaacgatt gcttgaacgc ttgatggtct
     3841 tccgcatcct ctaaatagtt atagtgcaaa gtaaacgaca tatcttcaaa cgaataatct
     3901 tgtactaagc cacctaaccg tcctaaaaca gaagtttcaa ctctctgtct ttttggagat
     3961 ggtatggttg gtctttcagc taaagccaat ttatgcaaat aatcaggaaa tccatcgatt
     4021 atagaatgta tacaatcagt cattttttca catccttttt aatacta
//
LOCUS       DAJSVF010000153         4058 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSVF010000153 DAJSVF010000000
VERSION     DAJSVF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.162"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            483..1808
                     /locus_tag="ON550_002794"
     CDS             483..1808
                     /locus_tag="ON550_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3955816.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            1801..2148
                     /locus_tag="ON550_002795"
     CDS             1801..2148
                     /locus_tag="ON550_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955817.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2463..3320
                     /locus_tag="ON550_002796"
     CDS             2463..3320
                     /locus_tag="ON550_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3955818.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            3340..3900
                     /locus_tag="ON550_002797"
     CDS             3340..3900
                     /locus_tag="ON550_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HCR3955819.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT         1536 a          547 c          754 g         1221 t
ORIGIN      
        1 gctaatcgaa gtaaaaccca aagccatatg aacccctgaa tcagcgaaag tttatgatga
       61 tttggattac taatagttta ggaattagat ggtaaatcta aattaaacaa tcgttgattt
      121 ggcaacgaaa tagtgaggga aaatccactc actgaagggt aaactccgtc aagttatgtg
      181 accagacgga aggacgaatg ttgttgaggt tttagaacca tataaaacag catagttctc
      241 aaattgttta aaatgattgt caagttaaaa aaatctggga tcatataaaa tagtatagtt
      301 ctcaaatatg attagaaacc ctattaaaga gcaatattaa gccaacttac ttaaatcgtt
      361 taattaagaa caataatctc ttttagtcag acttttagtc tgactttttt gtatttccaa
      421 aatttattct ttacaaaaac gaacatacgt ttgtataatt gcttgtgaat gggggaataa
      481 aaatgtttga ttacagtaga gaaccggtga atgactattt tttaatcgac atgaagtctt
      541 tctatgctag cgtggaatgt attgaacgaa atttagaccc aatgactaca ccgttaattg
      601 tcatgagtag atcggacaac actggttcag gtctgactct agcttcttca cctactgcaa
      661 agaaaaaata tcatattact aatgttagta gagctactga tttaccttat ccattaccaa
      721 aagaacttca tattgttcca ccacgaatga acttatatat aaaaaaaaat atggaagtta
      781 atcagatttt tagaaagtat gtcgcagatg aagatttatt gatttattcg attgatgaat
      841 caattttaaa agtgacaaaa tccttaaatt tatttactag ttcaggaaca agaagcgaga
      901 gaagaagaga attagctaaa ataatcaaag atgagattca gaaagaaaca ggtctaatag
      961 caacagtcgg aataggagat aacccattat tagctaaact tgcactggac aatgaagcga
     1021 agagtagtaa agaatttata gctgagtgga cttatgagac tgtacctgaa aaaatatgga
     1081 aaataaacga gatgactgac ttttggggca ttggatcacg catgaaaaaa agattgaatc
     1141 atatgggaat tatgagtata gaagatttag caaattggaa tccctacaca attaaaagtc
     1201 agttaggtgt aatcggactc cagttatatt tccataccaa tggaattgat cgtacggata
     1261 ttgctattcc accagaacca accaaagaaa agtcttatgg gaattcacaa gttctaccaa
     1321 aagattatac gaggagagaa gaagtagaac tagtagtcaa ggagatggca gagcaagtag
     1381 ccattcgaat tagacaacac aattgcaaaa ctggttgtgt acgcttagca attggtacgt
     1441 caattttgga agaaaaatca ggtttctcgc accagatgaa aataccaatt acagataata
     1501 cgaaagaatt acaaaattat tgtctttttt tattcaataa gttttatgag gggcaggaag
     1561 tacgacatat aggagtaact tatagcagat tagtatacac agattcccta caattagatt
     1621 tatttagtga tccaagaaaa aagattgctg aagaaaattt ggataggatt attgataaga
     1681 ttagacaaaa atatggattt acagctatta tacatgcttc atctatgtta gaaggtgcaa
     1741 gaagtattac gagaagcact ttaataggag gacacgcagg aggattagga ggtttgcata
     1801 atgattgaat ataacgatag aggacgttta aaatggtcgg gattttattt gtctgatcat
     1861 actgaaagaa tcaatgcaga caatgtacaa cgtgctaatc ataatttggc aaaagaacaa
     1921 atgacgactg aagagattgc gaaaatactg agaactgctg tcataaaaaa tagacccgtc
     1981 tgcattcaaa aagaggaacg aaacgctgaa ggttactatc cagatgatgt agtaggaaaa
     2041 attattggat atgacgagct tggtgtattt gtggaagaag aaaaagttca ctatgatgaa
     2101 attaggaatg tttctttctc ggaattatta aaatggagtc agatatgaac attattaaaa
     2161 aatacgaatt cggttatatt acgtatgaac agctaataga ggaattatgg ggatatggtc
     2221 aacaattaat taatgaagtg gggattgatt gcttttgttt ctatattgaa tccggagcag
     2281 gataccataa gaacagatac tatatatttc catatccacc aaaatagtct cttagatatt
     2341 tttaaatttt ctccaaatac tttcaattat tgtgaaatat ttccctttaa ttaatcaaat
     2401 tttttataaa agtaatatat aaaataaatt gcttgaagta ttatatttag atatcataat
     2461 ttatggaaga aaaattaatt aaaaaggatg agattataat ggggttactg gttccgagag
     2521 ctaatactta tactatacct ttaacgaaac gccaattaat tgctaaaaga tatcaaagga
     2581 ttactagagc catcaataga gagttttgga attctgaaag tgatactgct cacagtttat
     2641 acgtaggttc ttatgggaga ggaacggcaa ttagtactag tgatatagat ataatagttg
     2701 aattaccgat ggcagaattt gacagattca aaaattattt atcaaatggc ccgtctaagt
     2761 tattacaagt tataaaaaac gcatttcagg aaatactacc aaatagtgat attagagctg
     2821 atggacaagt tgttaaaata aatttccatg atggaatcaa atttgaaata gttccagctt
     2881 ttaatgaaaa agattattgg ggtgagtcaa aagggtttat ttatccagat tcaaacatgg
     2941 gagggaactg gaaagctaca aatcccaaaa aagagcaaga agccatgaaa ttaaagaata
     3001 caaaaagtaa taatttatta tatgctacgt gtaaacattt tcgacatgta cgagatacag
     3061 aatttacgag ttatcatctt tcaggaatag taattgacag tttcgtatat gaggctatgg
     3121 gaaattggaa attcgttgaa aataacagtg ggggtcaaaa cattagttct gtatcttatg
     3181 agacagcttt attagaatac tataactcac ataaagttat gggcggttta aatttatact
     3241 ctcctggaag caatcaattt gttaattctg atagtagtat catatgttta gaaaaagtat
     3301 taaaaaaaat agctttataa agttttatgg aggaacaaca tgggagacga aaaaaagcgc
     3361 ttggaagttc aattacgtga agcatatgga cgagtaacgt atacttacac cactcattta
     3421 aagcaagccg ataatattat ttctgacaat aaaattttaa ggtgtcttca aattggtttg
     3481 tcagccctta ctactggagg ggtacttagt accattatta ctgataatag tgagttaacc
     3541 tttgttgctg gattatgttc aacaatttct cttggattaa acttatactt taagaatttt
     3601 gatcttgctg aagaagctaa aaatcatcaa attgttgccg ataaattatg gttgatcaga
     3661 gaagaatacg taagcttact aacagattta aatcgtttaa gtgttgaaga tattgtgaaa
     3721 aaaagagatc aattaatgaa cgaaactcat gaagcatata aaaatgcccc taaaacaaat
     3781 agtaaaagct atgaatcagc tcagaaagca ttaaaatctg aagaagagca attttttaca
     3841 gaagaggaat tggataagat gttaccagag catcttagaa aaaagagttc taaatattga
     3901 actctttttt tatgcttaaa attctcttgt aatttttcta ttcatctata tttaagaaga
     3961 aatatgatga tagaaacatc tcctaaagcc accgcaagcc gttctaagca tagcttagcc
     4021 cgacattgct agatatgctc atctcaccat gaacgttt
//
LOCUS       DAJSVF010000154         4039 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSVF010000154 DAJSVF010000000
VERSION     DAJSVF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.163"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            179..775
                     /locus_tag="ON550_002798"
     CDS             179..775
                     /locus_tag="ON550_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3955820.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="ON550_002799"
     CDS             899..2698
                     /locus_tag="ON550_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3955821.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="ON550_002800"
     CDS             complement(2976..3188)
                     /locus_tag="ON550_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955822.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="ON550_002801"
     CDS             3650..3799
                     /locus_tag="ON550_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955823.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1187 a          689 c          712 g         1451 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaata
//
LOCUS       DAJSVF010000155         3992 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSVF010000155 DAJSVF010000000
VERSION     DAJSVF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.164"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..366)
                     /locus_tag="ON550_002802"
     CDS             complement(<1..366)
                     /locus_tag="ON550_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3955824.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKL"
     gene            complement(378..782)
                     /locus_tag="ON550_002803"
     CDS             complement(378..782)
                     /locus_tag="ON550_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955825.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(775..1176)
                     /locus_tag="ON550_002804"
     CDS             complement(775..1176)
                     /locus_tag="ON550_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955826.1"
                     /translation="MVNDNSKEKERLNKQISAIKTSLEEHFKLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(1166..1519)
                     /locus_tag="ON550_002805"
     CDS             complement(1166..1519)
                     /locus_tag="ON550_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3955827.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGE"
     gene            complement(1509..1820)
                     /locus_tag="ON550_002806"
     CDS             complement(1509..1820)
                     /locus_tag="ON550_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955828.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1817..2692)
                     /locus_tag="ON550_002807"
     CDS             complement(1817..2692)
                     /locus_tag="ON550_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955829.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(2702..3862)
                     /locus_tag="ON550_002808"
     CDS             complement(2702..3862)
                     /locus_tag="ON550_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3955830.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3862..>3992)
                     /locus_tag="ON550_002809"
     CDS             complement(3862..>3992)
                     /locus_tag="ON550_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3955831.1"
                     /translation="GTEAKKEQSVEEILNDVKEKRAEKIAAFTAALNKTFGQGDVK"
BASE COUNT         1140 a          816 c          593 g         1443 t
ORIGIN      
        1 taactttcta ttttcttcga agtcatccac gatctctgca gcaagtgaga aatcttcgcc
       61 tacggaatca ggaccgtaag agtaaatgcc tggtttaata cgtccatctt gtttcaaccc
      121 attgaaacgt cgataaactt ccatcggtac atgtgcagta attgttaccg tcatattgat
      181 tggtttagat tttgatttta cttctgtcgc tccacatttt ttaaccaccg tttgcatttc
      241 tgtttcgcca tctaattgtc cattacaatt cgttgcgatt gcatttcctg cgttcttaaa
      301 attaaaagca attcgtttga tacttacgtt atcgaacgtt gttactacag tttttgtttt
      361 agccattgtt gttcccccta tttatttaat ttatcgaatt gacgaatcag aagttctgta
      421 attggatcaa gtgcaagacc taatcctctt ctcataaatt cgtccggctg atttctttta
      481 gaagtaccta tccccaaatc aggatatttt aaatactcaa attttcttgt aggtctaatg
      541 atgaaaccca aattaatgta ttgagtctta agtggacgac tattttttgc gtgttggtgc
      601 cctcttctta aatctgcttc agaaacagga attttttctg taatcctatc cactgcaata
      661 gccgaacctt ttgatttcaa tgcttcgtta atcagtcgtt cgctctcgct tgaatagcgt
      721 tccatccgca caagaagttc atcatgtcca tttattttta gctcccaact atttttagcc
      781 atgacaatca ctcttcaata atcgtctaaa cgtaaatacc aattgatcga tatagcgatc
      841 ttgattttct agttttaaat ggttgggatc cattctctga aaacgaatcg aacgattttg
      901 aatcaatgaa ataatatcta gtgaatctcc tgttaaatct tctctatttt ctgaatagaa
      961 agttagatat agattttgac ccacactata ttttggttca gtgatcattt ctatttctcc
     1021 tgtttcgaga atgaagtaat taaaatcatc aggtagctca tcctcgccaa cagagtcttg
     1081 aaagagtttg agcttaaaat gttcttctaa ggaagttttg atagcagaaa tttgcttatt
     1141 taaacgttct ttttctttag aattatcatt caccatattc acccacactt tcaagataaa
     1201 aatagatata aaaattatcg taatcggcat agataacgtt gtaacgcata ctatcgatta
     1261 cgataaaata ttgatcttta ttaaatttct tggcgattgg atgaaatgga gtctttactt
     1321 tcttagttaa tttcgatccc atcgcatcca tagctgttat atcactatct ctcatggaaa
     1381 gatttctaaa ttttaaagaa gtgatttctg tatcttctac accaatcttt tttcctagtt
     1441 catttctttt ggtagtttgc gtcaaaatct ttaaccaacc atcgttgaat gtttcttcga
     1501 gtctacgatt attcgccatt cacatcacct gcaatatatt cttgtagcgc ataatgttga
     1561 atgaaaccta ataactcact agcgaaattt tgttcaaact catctaaagc acgattccag
     1621 tcgtatctac atctttcgat tagcaatccg tattctaagc tttcaggaga aaaagaaagt
     1681 gttgtactca ctttactttg aagataaaca gcatttttag ctatcatctt tttaattgac
     1741 tcatcttctt cgttccaggt aacgtaaata ttatccttca cagctattag caattcttca
     1801 gtcacttgtt caggcgtcat ctaaccaccg ccttaattgc tttaacatat gcgtaagagc
     1861 attttttctt gtttacaaat gataaatctt catcaaaagg cgtagaagtc acgtatctcc
     1921 ctttgaaaaa taaatcttca tcgtttgttg ttactccagc attgtgtaag atttttactt
     1981 ctttaacttt ttctattgga tcagtagcaa aacaaaagtc taattcctcg tgaactttag
     2041 gaccaatatt gaaatacatc atgttccaaa gctgtgccca catctcggct gtccagattt
     2101 gtatatttgt tttttgccct ctaaggtagc gatatagccg attagaatcc agataaacct
     2161 ttttccaata attcgcttta gggcggttaa taacccactg tgcgcctcct gaattagtgt
     2221 ttatagtttc caaagattct actgtaacat ttacaatgtt tgccatatct tttagaatat
     2281 tttctccgtt ttcacagctt ctaatataat caagacttag ataactacag cagtcgctac
     2341 aataccaaac atcatcttta gaaggcaatt tgcgcaaatt aattctttta ttgaaaatga
     2401 catccgaatc gatatagaaa tatcggtcgt cctcacgcga atgatcttct tctaaatatt
     2461 tccaccataa atatggttta atcgaaggaa tatactcttt gtcgtcccgc agatcatcgt
     2521 acacatgaac ttcaacacca tattccttct caaaaaaaat aggaatctga tcatcgtgtc
     2581 tgctgaaaag caatacgata tctttgattc ctagtttctt cagattagtt aaacaaactt
     2641 caagctccca tttaaaccga ttgattgccg gctgacaaag aatatacttc attctgatca
     2701 cctacgcttg tgttgtagtt gttgtggttg ttggttttgt agttgtagta gtagttccca
     2761 aagcgctaat atctaataca atgaaactat cgttacgttt aggttgaccg tttgcatatt
     2821 gtttagctag ataaatgcgt tcgtcttcaa caaaatggta ttcatctgaa gcttcaattt
     2881 ttagtgtaga tcctacaccc atgaagtaat ctgaggctac cccaataact gcttttcctt
     2941 ctggcacagc cgttgactgc aaatctgaaa ctggtactgg caatacttgt acgtattctc
     3001 cattagcagt tagtacagtc ttagctggga atactttaga ccagtaatca gttggattca
     3061 caattaggac cacatcagaa ggattcacat tacgataaat cggatcattc acaccttcga
     3121 tattgaattt tgatagtcgc gccatcaaac cgcccatagt tacagcatct aaagctgtaa
     3181 taggttctgc ttttttttca gcatattctc cgctagtttg tttgctcatg tcacgcatca
     3241 ttccgactgg catatcttta ccagtaccat caacaattgc ttgttctaat gcaattctca
     3301 atgattctac taaaacagta cggacataac gatctaacca tactggacct aaatcaagca
     3361 ttgccttaca tacaggaata taacctgata gcttgaactg cttcatgtta attacatcaa
     3421 agccattatc taaaactttt ttaacagctt cgcaaagttt accccaccat gctggattga
     3481 ctccacgtga cacaatccat tctgttacac cagttgtgtt aacaaaagta attttttgca
     3541 atagtggatg agattgttct aaatcttcaa atacacgttc aaatacagta gctggcacta
     3601 attcttcgac ccctgcaaaa ccttcgtttt tcactacttc gttatagaat tttgtttctt
     3661 gtgtagttaa tacacgctga ccacggttca ttaatactaa ttgatcttga ttttttgctg
     3721 ttgcttcttc taaaatttta tcctgaattt ccttagataa gcttaccata gctgcgctaa
     3781 aagattcttc gttaccatct ttaaaagctt tcatcaattg gtcgcttgca gctgttacac
     3841 cttttaaatt tttaactgtc attattttac atctccttgt ccaaatgttt tatttaatgc
     3901 tgctgtaaat gcagcaattt tttctgctct tttttcttta acgtcattca aaatttcttc
     3961 aacgctttgt tcttttttag cttcagtacc tg
//
LOCUS       DAJSVF010000156         3976 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSVF010000156 DAJSVF010000000
VERSION     DAJSVF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.165"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(80..640)
                     /locus_tag="ON550_002810"
                     /pseudo
     CDS             complement(80..640)
                     /locus_tag="ON550_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369693.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            874..2166
                     /locus_tag="ON550_002811"
     CDS             874..2166
                     /locus_tag="ON550_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3955832.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCLISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYIRRLWRPGFKAHLTETDIVDRLLKRSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2454..3938)
                     /locus_tag="ON550_002812"
     CDS             complement(2454..3938)
                     /locus_tag="ON550_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HCR3955833.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKDFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISHSIIANRYDSILNDVKEKVYTRDLFQRD"
BASE COUNT         1211 a          709 c          706 g         1350 t
ORIGIN      
        1 agagagtggg acaatagtcc tttctccaaa agaaaacaag tccgattttg atggttaatt
       61 tcaaaatcgg acttgttttt tcttctttta aataaggcaa gatttcttct ttataaaggg
      121 cataatagaa ttcgtttgat tgcttatcca aagcagtttc ataacgattc gtttctttac
      181 gccagaagaa actgaacttg ttggcatctg cttcaatttt tgtaccatca ataaatattg
      241 cttcattatc aattacttaa ttggtgatcg attggcggct aaataagaca aacgcttcag
      301 ctaaaaggtc agcgggtgtt tcctgacttc ggaatcgatt gatggtccga tagctgactt
      361 gttcgtggtt agccaaccaa cacatacgat agctgtcatc taatagaaat tcaattttcc
      421 gaccagaaaa gacacgctgc atataagcat atagaataat ttttaacatc attttggggt
      481 ggtaggccgg tcgtcctagt tattcttata attcaagaaa gagtgtttgt ggcattgcta
      541 cgactaaagc gttgactgca aaagcgacat ctgtttcagg aatatattgt tccatatcta
      601 atggtaaagt tgtttgattg atgggataat ttttaaacat aaagtactcg cttcctttct
      661 gattgggctt ggtcacttta atcttacagg gatgagtgct ttttgtgtac cttaaaatca
      721 aaaaaagtgg ggcaataatc agattttggc tctatacttt ttgatgttgg tggatgacac
      781 cagcatcttt tttattttcc aaaacatata gaaaagacac cttgaaatcc tttagaatta
      841 atttggcgaa ccaacccaaa aggaggaatc aagatgtctt acactcacct tatcaaagaa
      901 acattagata ttctagactt atcagttact tttaacgaaa attgtctcac aaaagaaaaa
      961 tacaaagggc aaatctgtca tatttatcgt ggaaacttga tttatacagc ccaagaatgc
     1021 atccactgta aacaccagat tgcttcagat attgtgcgtt ggggtacaac aaccgtccgg
     1081 ttgttaatga acgatgtttc tgaataccgt acctacctcg aattgaaaaa gcaacgcttc
     1141 aaatgcaaag cttgtcagcg gacctttgtc gcagatactt ctgttgcgaa aaaacattgt
     1201 ctcatcagtg agaaagtccg ctggtctgtc gtgactcgtc tgaaaaaaaa tacctcaatg
     1261 acagagatcg ccgctcagaa aaatctatcg gtctcttctg tttaccgtat tatgaagcga
     1321 ttttatcgtc cgttgaatcc ttttcggaca ccgttaccta aggtactttg ttttgatgaa
     1381 ttcaaatcag ttcggggtgt ttcaggtgct atgagtttca tcatgatgga tggacaaaca
     1441 cagcgcttat tggacattgt cgagaaccga caactgcctt ttttaaaacg ttattttagt
     1501 catttttcac gagaaattcg tgaagcggtc gaatggatcg tgattgatat gtacgctcct
     1561 tatgtatcgc tcgttaaaaa gctgtttcca aaagcacagc tgattattga ccggtttcac
     1621 atcgtccaac atattggtcg tacctttcgc aatcatcgaa tcaaagaaac gaaccaacta
     1681 ttaaaaagca aagaacaaaa acactatcaa ttaggtaagc aattaaaaag atactggaaa
     1741 cttcttcaaa aagatgaacg caagcttgat tatattcgac gcctttggcg acctggcttc
     1801 aaagctcatt taacagagac cgatattgtg gatcgcttac tgaaaaggag ccctgctttg
     1861 cgtgtaggct atcagttata tcaagacttt ctttacgctg taaaggaacg agactacgta
     1921 tcgtttgaag aactacttac caataatatc atgcttcccg aaggctatca gacgaccttg
     1981 agaacgttcc agaaatttct gccacaaatc aaaaatgctt tacaacaaag ttattcaaat
     2041 ggcccacttg aatgtctgaa caaccatatc aaagtgttaa aacgaaatgc atatggcttt
     2101 cgtagctttt ataatttcaa attgcgcatc atgattcgtc atggcaacgc attgattttc
     2161 aactaaaaaa gatcaagagg aactgatcca cttgatctcc aattattcta agatttctct
     2221 ttaccaacat catttgacaa agagcccaga tttttttctg actattgtcc cactcccctt
     2281 gcgttgtaaa aattttgata attagatttc ttaaagttgg taatctttta ttaaaaatct
     2341 tttctaaaga gagacgtaat tcctcctagc gcttaattaa tgggtctaaa tgaaaggata
     2401 aaaattagtc ttgctgttta ttgtaaatta ttttaatgta tctaatcatt cgtttaatcc
     2461 cgttgaaata aatctctagt gtatactttt tcttttacat cattaagaat actatcatat
     2521 cgattagcaa taatactatg acttatgctt ttaaattttt ctaaatcatt tattactttt
     2581 gaaccaaaaa atgtactacc atctttaagt gttggctcgt aaattatcac agtggctccc
     2641 tttgccttga ttcttttcat aataccttga atagaagatt gacggaagtt atccgaatta
     2701 cttttcattg taagcctata gactccaata atcacttctt tttctagaga caaatcgaaa
     2761 gtattgtcat catcgtaaga ataatatcca gttttttgaa gcacgcggtc tgctataaag
     2821 tcttttcttg ttcggttact atcgacaatt gcactcatca tattttgagg tatatcgtta
     2881 tagttagtta gaagttgttt tgtatctttc ggtaaacaat agcctccata tccaaaagat
     2941 ggattattat aatgtgtgcc aattcttgga tctaagcaaa ccccgtgaat gatttcttgt
     3001 gtatttagtc ctttcatttc tgcataagta tccagctcat tgaagtaaga tactcgaagt
     3061 gcaaggtaag tattagcgaa taatttaact gcttcagctt ctgtgaatcc catgaaaaga
     3121 gtttcgatat cttttttgat tgctccttct tggagtaaat ctgcaaaagt gtgagaggct
     3181 tctcgtaaag ttttgtcagt ttggtccgtg ccgacaataa ttcgcgaagg atatagattg
     3241 tcatataatg ccttagattc tctgagaaat tctgggctga atagaatatt tttagtattg
     3301 tattttttac gaatactttc ggtatagcct acgggtaccg tcgacttgat gaccattatt
     3361 gcctttggat tgacttctaa caccaatttg attactgact ctacagcaga agtatcaaaa
     3421 taattttgtt tactatcata atttgttgga gcagcaatga caacaaaatc ggcttctcta
     3481 taggcggctg caccatcagt cgttgcaatt aaattaagat ttttttcagc tagatatttt
     3541 tcaatgtaat cgtcttgtat tggagatttc ttattgttaa tttgttttac tttttcagga
     3601 agaatgtcta ctgctgttac ctgattgtgc tgggcaagaa gagtagcaat ggaaagacct
     3661 acgtaaccag taccggctac agcaatattc ttcgactgat gttttgttct gtttgattta
     3721 tttgattcat ttataaatag atctgttggt tcaaaattta gaacttctgc taatgattct
     3781 aattgttgta ttgacgggat aaaatcgaga gattctactc gactaattaa ggcacggttg
     3841 ataccagtag ctactgcaag ttgttgttgt gtcattttca gagattttct agaatcaata
     3901 atgatttgag ctaaacgttt ttgagataac tttttcatga tgcactcctt tttttgttgc
     3961 taataataac aaacaa
//
LOCUS       DAJSVF010000157         3914 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSVF010000157 DAJSVF010000000
VERSION     DAJSVF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.166"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            112..405
                     /locus_tag="ON550_002813"
     CDS             112..405
                     /locus_tag="ON550_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3955834.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
     gene            631..1449
                     /locus_tag="ON550_002814"
     CDS             631..1449
                     /locus_tag="ON550_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3955835.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1468..1875
                     /locus_tag="ON550_002815"
     CDS             1468..1875
                     /locus_tag="ON550_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955836.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            2103..3188
                     /locus_tag="ON550_002816"
     CDS             2103..3188
                     /locus_tag="ON550_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3955837.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT         1526 a          561 c          631 g         1196 t
ORIGIN      
        1 tatctctatg aatccttttt tgtttttaca aataggagta gatactagaa taatgcgtgt
       61 gttaaactat tattgcaaac gcaataataa aagaaagtag gcttacgcac catgtctatc
      121 aatacatttg tttactcgca cccgatcaat gtttacatta tcaagaattt aggaatcact
      181 gtagaacaat tttgtgagtt atatgcctac ccacaaggaa cggttgcaag ctggataacc
      241 cgacaacgta gaataaaatc attacctgct tcatttgtat atgatctaag tcttgcttct
      301 agtatgaata tgaccgatgt atacgaaaag ttattaatac tagaaaatga atatgatcaa
      361 ttcaattcaa atcagcagcg aaaagtaaag aaacagatag actagaatag ctattttcga
      421 aaagcaatat ttaaggtata agttatacct taaataataa aagtataagt tataccataa
      481 atatatatgg tataacttat acttttatta ttaaaaaccg ctatatccaa acgtttttag
      541 atattattat ttaagttata cttttaatgt tgaccatttc cgatatataa gttatacttt
      601 aattatcaat taaagattgg gaggatttac atggatactt ttcaagggac tacatttaca
      661 gttggcaatt ttaaaggagg agtaggtaaa actaagatag tcagcatgct tgcttatgac
      721 aatgcaatga taagaaatag aaaaacgctt attattgatt tagaccctca agcgaactta
      781 acacaagtca tagcaagaac tttcggtata accgatatca aaaccactat aacaaatggt
      841 gtcgcaaatg gagaattagc aagtagtatt attaaggtaa atgaaaatct tgaccttctg
      901 gcatgtgaca ctagttttcg atcattctcc gcttttgcta ataaacatga aaatgaatta
      961 gaccaagtta ctgtcttgaa aaaattactt cttccaataa agaacaatta tcaagaaata
     1021 tttattgatg taccaccaac cattagcgaa ttttccgata atgctatggc agcttcagac
     1081 tattcgataa tttcatttca aacagctgaa gaatccttag agggagtcaa caaatatgta
     1141 aattatcaaa aattcatggt cgatagatac gatcttgatt tgcaaattat agatattgta
     1201 ccttgtatgg tagaaccaaa tgatgatttt gatgaagaaa tcctagaaga agcaattgaa
     1261 aaatacggaa ccgtggtatc caaaaatctc attcattatc aaaaaagatt gagaaggtat
     1321 tctaaaattg gtatacattt aagaaaatac aaaaatggaa attttgatca atgggattat
     1381 aaagcacacc aagtatttat aaatattttg gcagaactag atgctagaca agagttttta
     1441 aataaataat aaggagttgc ataagtaatg cctaaagatt ttgatattcc taaaatgatg
     1501 gagaatggtg aatttgagcc attattaccc aataacaaac gccaagatgt gaaaataagc
     1561 ggtgaatata actttagcaa tagtgcattg agtaacacaa gaaatattac attgaaaaaa
     1621 agaggccgac cagtttcaat tcctgataaa agaaacaagg tatcagttcc taaaaagatc
     1681 tctccggcat taaatagcaa actctcaatt ttacaagact atatgacgga gttacaaagt
     1741 gaaaaaggga gaatttcttt cgaaaaaatt gttaatacat tagcagactc atatatccag
     1801 catagactag gagtagcaaa agaagaacac ataagagaag aaatacaaga agagtttgaa
     1861 aaattaccaa aataattttt ttcatgcggt attcaattaa agtataagtt atactagata
     1921 aatgaatatt aaagtataag ttatacatta tttgtattta aagtataact tatacttatt
     1981 tttttaatat ataacttata ccttaaatac tgaaagtgtt ttattcaccc ttaatacatg
     2041 gtatagttca cttgttgaaa atcctgaata tatacaagtt tctaagaaga aggagaaatt
     2101 taatgtctac agaaaatcaa tctcaaaaaa tgataattaa aaattgggga tataaagcaa
     2161 cgaatgttta tattgaaaaa tattataaat ttcctagttt tttaattcag aattctaaat
     2221 actctaaaat gaaagacagc tcaaaaatag cctatatgct ttttaaaaat gaatttcgta
     2281 gagccttaca tgaaaaatgg atcgacgatg atggtctatt atatttggaa tttacacaag
     2341 cagacctaat ggacaaatta aattgctacc aaggcaaagt aaaaaccatt ttaaacgagt
     2401 tagaaacata taatttgatt tcagtcgtaa aaggagaatt taacccaaaa acgaaaaaaa
     2461 atgaaaaaaa taaatactat ttacttcaac ctgaaataag tgccgatgat ctttttattc
     2521 aaaattcttt cgatgacgat ttaagcgaaa ctattgataa atcaacctct tctcggaatg
     2581 cggaaatcgc acgtcgagag gaaactgcta aaacccttga taacactgga aatgcggaaa
     2641 tcgcacgtcg agaaaaaaca gctgaaaccc ttgataacac tggaaatgcg gaaatcgcac
     2701 aagattatta tattaataaa tctttagaca ctaaaagaca cttaaaagac actgaaacag
     2761 accaattaca aaatcaagtt ttgctagata acttcgttga aatcatgaaa gaagacagca
     2821 taaacacatt tgtaccagaa aaggtattaa gcttgataaa aacattttct aacacttacg
     2881 aagaagccca acaaaccgtc accacaatac acaacgcaaa acataaagcc gaggaacttg
     2941 agaagacggt agttgtgtat gaagaacttg aatcttacgg aattgatgcg gataaaggat
     3001 tatatatgac gttgctgaaa gcttatcaaa aacaaaagac tgagaaagta aataatttgc
     3061 agaatttgat ttttgtgtat gtaaaaaact ggtttattga aaaagctatt gcggcaaagc
     3121 ttgcaaacga acaaagagaa acatttaacg aattaccaac agttagtacg gaaaattggc
     3181 tagagtagtt gataaaaagc gaacactata aaaatgatca attttaaatt agaacttttt
     3241 tacaatttta attttattac aaatgttaca aataaatagt tattattccg gagtttttag
     3301 attaaaagtt tatgaaaaca atcacaaacg taggttttaa tgtcataatc gttagatatt
     3361 ttagttattt ttcattcaaa aaaacaggga actagtgttt gcatatgtat ttttttgagt
     3421 tataataatt catgaagaga ggtaattcac ttatttaccc aagctgaaaa gcctgatatt
     3481 acttcttttc aggcagtgct gctaatattc aattacactt ttttgtataa atttagttgt
     3541 tttaaattta attgtctgct atgcttgtga tgaattgatt ttcgttctgc acttttggac
     3601 gataaatttg gacaattgaa aatacaaaaa aaaagaaagg ccctgtgaga ctcggaaggc
     3661 taccaacctt cgtcgacgtc cacaggtggc attaagatca ctcccttaat gcgtttacct
     3721 ttcagtgtta cggtattacc cgtaacggat ttagtataac agatattcaa aagtgatacc
     3781 actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag
     3841 cataatgtta taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag
     3901 ggaacgcatg tttg
//
LOCUS       DAJSVF010000158         3892 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSVF010000158 DAJSVF010000000
VERSION     DAJSVF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.167"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ON550_002817"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ON550_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR3955838.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="ON550_002818"
     CDS             complement(3250..3768)
                     /locus_tag="ON550_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR3955839.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1256 a          635 c          589 g         1412 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat ga
//
LOCUS       DAJSVF010000159         3759 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSVF010000159 DAJSVF010000000
VERSION     DAJSVF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.168"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..125)
                     /locus_tag="ON550_002819"
     CDS             complement(<1..125)
                     /locus_tag="ON550_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR3955840.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ON550_002820"
     CDS             complement(143..463)
                     /locus_tag="ON550_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955841.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..1538
                     /locus_tag="ON550_002821"
     CDS             762..1538
                     /locus_tag="ON550_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3955842.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1553..1756
                     /locus_tag="ON550_002822"
     CDS             1553..1756
                     /locus_tag="ON550_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955843.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1772..2110
                     /locus_tag="ON550_002823"
     CDS             1772..2110
                     /locus_tag="ON550_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCR3955844.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2097..2276
                     /locus_tag="ON550_002824"
     CDS             2097..2276
                     /locus_tag="ON550_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955845.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2319..2789)
                     /locus_tag="ON550_002825"
     CDS             complement(2319..2789)
                     /locus_tag="ON550_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955846.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2876..3574
                     /locus_tag="ON550_002826"
     CDS             2876..3574
                     /locus_tag="ON550_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR3955847.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
BASE COUNT         1402 a          572 c          684 g         1101 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
     1021 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
     1081 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
     1141 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
     1201 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
     1261 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
     1321 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
     1381 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
     1441 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
     1501 gatattaccc tcaaatggat ttagaggaaa ttggttagaa aggagtttta gtatgactga
     1561 cattgcagaa atcactcaac gagatagaga aaaaatcaaa gaatatgtcg aaagttcaaa
     1621 gttcttaact tacaccatgc ttgctgaaag atttggaatt agcaaaagct acttatcttt
     1681 aattttaaac ggtaaaaaga cttctgcaga agcaaacaga attatagatt cgattatcac
     1741 tatgtacgaa ttgtaaaggg aggaacagct aatgcaatat ctagaagcaa aaatcccaat
     1801 tccagaaggc tatgtaatta tctcccaagt ggattatgag gagttaaaaa aagctgatga
     1861 tactggtaga tggatgacgt tgccagaagt gctagaacgg attaacagaa aatatgattg
     1921 gtttacttct agagttttaa agaacccaag atatagaaat attatcgata tcgagaaaaa
     1981 caaaaatgga tttgtctatt atccagttga aggaagagac acatatctat ttttaagaag
     2041 taaaacactt gaatttttag aaacaaattt ttcggaaatc ttaaggaggc aagcggatgg
     2101 gaaaatttaa tagagcgcta gtgttcagcg caccgctaat catctacgct ttaggacttt
     2161 ggggaagcag gcaagcgttg ataggaacga ttgtttacat ggtttggatt tttatggggc
     2221 ttgatgaagc tgaagctgag tacagagcga aaaagccaac cgagggggct gactaaaaat
     2281 gaatagaaaa gagaaactag aatgtatatt actattactc agtttaattc tttcactaat
     2341 ttctctattg ggaagtttgt atttttgata tcaacaattt tgtggatttg ggttctattt
     2401 agagtaaata tcatttttgt ttggtgatta aggagtttat taaaattaat tttccctact
     2461 tcaaatgcaa ggtaacaaga tatagcactt ttaggaggaa tatttagcgg tacagcttca
     2521 gagtatattc tattatcatc gactgatcct cctttagcaa ttaatttctt aaatggagta
     2581 gctgaccatc tgcggtttaa gttagcggat gaaagttcta aattaactaa catagctggt
     2641 tctgttgaat agttagaaat gattactttt gtataaatca tatctgcagc gaagtaagaa
     2701 gcattaagtt caacttctag ttgagggcgc attcctttta tttttatccc tgttaaaaac
     2761 gtactcaata taaaacttat tattgacatc cattgaaaaa ttgttaagtg aaaaaaattc
     2821 aaaataaaca ccaccagttt ttaactaaat tataccaaaa aggagagaag aaataatgca
     2881 agaattagta attttgaaaa ataaagaagc tgtgactacg agcttacaag tggcagaaag
     2941 cttcgagaaa aaacatcaac atgttttaag agatattgat gcactaaaaa aagatgtgtc
     3001 caattttgga cagatgtttg tagaaggtaa tgaaccagat tcatatggca gaaatcgacg
     3061 agttttcttc attagtagag atggattttt cttgctggct atggggttta caggaaagaa
     3121 agctatctac ttcaaacaaa aatacattga agcattcaac gaaatggaag atgttattcg
     3181 caagaatact gttcctcaaa caattgaaga tatgatgatc tatcaattag aagaaatgaa
     3241 agatgttaaa aaagatgttt ccatgcttaa agatactatg cgaattagcg gacaacaaga
     3301 gtttgaaatt aagcaaaaag gaaatatgaa agttatggga gttctagggg gaaaagaaag
     3361 ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg
     3421 taccttttca atcccaagat atggcgagtt acctcgtaag agattcgatg atgctgtttc
     3481 atttattgaa atgtggttgc cagaaactgc gatccgtatg gaaattgatc aactgaacag
     3541 acaacagaga ctttttggtg atgacaatga atagagctga agcgctaaga atagggacgg
     3601 caattgctaa ttgctggtgg aaatactata aaccaatcat cctaagccaa caacatattg
     3661 acaagcaaaa agcatggcaa caaataaaaa agtgactccg ccggcaagca aagagtcaca
     3721 aagaaaacac atcataagga gattttagca tatggaaaa
//
LOCUS       DAJSVF010000160         3690 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSVF010000160 DAJSVF010000000
VERSION     DAJSVF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.169"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..467)
                     /locus_tag="ON550_002827"
     CDS             complement(<1..467)
                     /locus_tag="ON550_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3955848.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDP"
     gene            628..1317
                     /locus_tag="ON550_002828"
     CDS             628..1317
                     /locus_tag="ON550_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3955849.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1331..2833
                     /locus_tag="ON550_002829"
     CDS             1331..2833
                     /locus_tag="ON550_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3955850.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2846..3322
                     /locus_tag="ON550_002830"
     CDS             2846..3322
                     /locus_tag="ON550_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3955851.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(3419..3529)
                     /locus_tag="ON550_002831"
                     /pseudo
     CDS             complement(3419..3529)
                     /locus_tag="ON550_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1219 a          517 c          676 g         1278 t
ORIGIN      
        1 gggtctagta cagcttttgc ttgaactcct acgcgaagaa acatattttc taaatcaaga
       61 ctagtatttc cactagatcc cactccaaat atatatatgt tttttgattg agtgatcatt
      121 ttaatggctt cattcaattt ttcttcgtct aaaagcctaa tagtggaata gagagcttct
      181 gttaaactat tagcaatttc atcatagtac tttacactag tcggatgttc ttttttttga
      241 ctaaagtctt cctttgcaat ttcaattttt aaatcagaaa aaccagaaaa accaattttt
      301 tgacaaaagc gaataatagt agcatcgcca acttttgtgt ttttcttaat atcactcatc
      361 gtactataaa taatagtttt cccagaattt aaaataaaat cagcgacttt tttttcagac
      421 ttcgttaaaa atggataatt ttctttaatt aaatagatat aactcataca aactcctcta
      481 caatttcaac ttttctccat tatactccaa aataataaaa aaaactccta aatactccat
      541 ttttatcttt acttttatga aagcggatgc tatattgttt ttggagttgt tatccaaaaa
      601 taataaaaaa ttggagtgtt taggataatg gataaaaaga ggtttttaga tcttatttca
      661 aaaggtttga ttgtttcctg tcaagcatta cctggtgaac cattttacaa agaggaagaa
      721 agtttaatgc cttattttgc tttggctgct caaagggctg gagcagttgg tatacgtgca
      781 aattctgttc gagatattat tgaaattaga aagtgtgtat cattaccaat gattggaatt
      841 attaaaagag attatccacc agaatctcca ttcattactg ccactatgag agaagtagat
      901 gaattagtag aaaccggtgt tgaggtaatc gctcttgatt gcactcttag aaatcgacat
      961 gatggactat ctataaattc ttttattact aaaataaaag aaaaatatcc tcatcagtta
     1021 tttatggcag atatttctac atttgaagaa ggcaaaaatg catataaagc aggaatagat
     1081 tttgtaggaa ctactttaag tggatatact gaggaaagta aaaaacaaga tgggccagat
     1141 atagatttga ttaaaaaatt ggtagaagaa gggatttgtg taattgctga gggaaggatt
     1201 catactccag aacaagcaaa agagataaaa aaaataggag ttgcaggtat tgttgtgggt
     1261 ggtgctatta ctcgaccaca ggaaattgca tcaagatttg ttaaagcatt aagttagggg
     1321 gagaaataaa atgtttaaaa aattttcaca attaggacgc gctttcatgt taccaattgc
     1381 tatattacca gtagccggat tactattggg actgggagga gcgttaacaa atgagagtgc
     1441 tataaatgct tatccatttt tagatcaacc gtggttgcat acaattttaa gtattatgag
     1501 ttatgctgga aatgcagttt ttgcaaacct agcactaatt tttgcaattg gtattgctgt
     1561 aggacttgca aatggagata aaggaacagc tggtttagct ggtggagtag cttatctagt
     1621 atatactgca actattagtg ggtttcttga acttttttca gcaaaagatg caacactaga
     1681 tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt gcatttttac acaatcgtta
     1741 tagaaaaatt gaattaccac aattcttagg tttttttgga ggatcaagat ttattccgat
     1801 tgtttctagt ttagctgcaa tcattattgg ttctttcttt tatttaattt ggccaccaat
     1861 tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa atgggtagtt taggaacttt
     1921 tttatatggt tttttattgc gtttaacagg tgctgttgga ctacaccaca ccatttaccc
     1981 tttgttctgg tatacatctt taggtggtac cgaaactgta gctggatcaa caattgctgg
     2041 agcccaaaat attttctttg cccaactagc tgatccaaac cacactggtc tgtttactta
     2101 tggtactaga ttttttgcag gaagatttgc aacaatgatg tttggattac cagcagcatg
     2161 ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa aagaatggtg ggctttattt
     2221 tagcggggcc cttacatcat ttttaactgg aattactgaa ccagttgaat atatgttttt
     2281 atttgttgct ccttggcttt atgtgattca tgcattctta gatggattat ctttttattt
     2341 tgctgatatc ttaaatattc gtgtagggaa ttccttttca ggtggtttga tcgacttttt
     2401 attattcggt gtacttcaag ggaatgacaa aactaattgg ttaaaattaa ttccttttgg
     2461 tattgcatgg gcatttatat atttctttgt ttttacattc tttatcaaaa aatttaaggt
     2521 agctattcct ggaatggaag aagaatcaga agttcattct gaaaaagtaa taaacaaaaa
     2581 tacatcatta catgaagagt ctcttatcat tattgaagct cttggtggaa aagaaaatat
     2641 tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct gttacgaatg gggagattat
     2701 tcaaaaacaa acaataaaag acactggtgc aactgctgtc ttcgaagtga agggtggcat
     2761 ccaagctgtt tttggaggga aagctgactt attaagtcaa gaaatcaatc aaatattagg
     2821 aaaagataat taggaggttt ttaatatgtt tgaaattttc aaaaagaaaa aacaagaaac
     2881 tttttatgca ccttgttccg gaaaaatcat ttccatcaca gaagtaaatg atcctgtctt
     2941 ttcacaaaaa ttactaggtg atggttttgc cgtcatacct gatggttcaa aaatttatgc
     3001 tccattggct ggtcgagtaa caaatatatt tcctactaaa catgctatta atataaaaag
     3061 caactcaggc attgattatt tgattcatat tggaattgat acagttgaat tacaaggaga
     3121 gcctttttcg atttgtgttc aagaaaatga tgaggttact cctgaaacct cgttggttga
     3181 tgtggatttt gacaaaataa taaaatctgg taaagatact tctgttatag ttgtttttcc
     3241 ggagaaaaat cagttcaatg atttaataat agataatggt cgaattgctc atgggaattc
     3301 atgtggaaaa atcacttttt aaaaaagtgt atataaaaag ctataaaata agctaaatag
     3361 gatatttgtc aaatgaagtt ggtgaacaga aagaactgtt ttctttctgt tcattttttt
     3421 attttattag taatgattcc ccatgccaaa tcatgatccg taatttaaag ttataaaagc
     3481 ttcgaaatcc attggcattt cgttggaata ccttgatatg attattcaaa tataaaatat
     3541 agagagaaga gagagaggga agatatttat aaagggcaaa gttatgttta ctcaacttag
     3601 gaaatagaaa tagatcaatt tatattctct tgtcaatcga atatgaacct tagcactata
     3661 taccaaatat agtaaaatgc tgaaagatgt
//
LOCUS       DAJSVF010000161         3677 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSVF010000161 DAJSVF010000000
VERSION     DAJSVF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.170"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            61..2502
                     /locus_tag="ON550_002832"
     CDS             61..2502
                     /locus_tag="ON550_002832"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR3955852.1"
                     /translation="MKEVVMIDAARTPIGKYRGGLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2832..3536)
                     /locus_tag="ON550_002833"
     CDS             complement(2832..3536)
                     /locus_tag="ON550_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955853.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1173 a          652 c          809 g         1043 t
ORIGIN      
        1 gaacaagagc tatttgtttt tacagcaaag aatggtatat ttttgtagga ggtggcttac
       61 ttgaaagaag tcgttatgat agatgctgca agaacgccga taggaaagta tcgcggaggt
      121 ttaagtccat ttactgcagt tgaattaggc acacttgtga ctaaaggatt attagataaa
      181 acaaaactga aaaaagataa aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt
      241 aatggtcaaa atgttgccag acagattgct ttgaacagcg gtctccctgt agatgtccca
      301 gcaatgacga tcaatgaagt ttgcggatct ggtatgaaag cagtgatctt agcacgccag
      361 ctgatccaat taggtgaagc agaacttgtg atagccggcg gaacagagag tatgtctcaa
      421 gcacctatgc tgaaacagta tcagtcagaa acaaatgaat atggtgaacc aatttccagt
      481 atggtcaacg acggattgac tgacgcattt tcaaatgcac atatgggatt aaccgcagag
      541 aatgttgcaa cacaattttc tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc
      601 cagttgaaag cagcacatgc tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc
      661 aagatttctg atgaagacgt gttatctgag gatgaagcag ttcgtggaaa tagtacattg
      721 gaaaaactgg gcacgttacg tacagtattc tcagaagaag gaactgtaac agcaggaaat
      781 gcttccccgt tgaatgacgg tgcctctgtg gtgatccttg catccaaaga atacgcagaa
      841 aataataatc tgccttattt agcaaccatc aaagaagtag cggaggtcgg tattgaccca
      901 agtatcatgg gaatcgctcc aatcaaagcc attcaaaaat tgacagatag atcaggaatg
      961 aatttatcaa caatcgatct ttttgagatc aatgaagcat ttgcagcttc ttctatcgta
     1021 gttagccaag aactgcagtt agatgaagaa aaggtcaata tatatggagg agcaatcgca
     1081 ttaggccatc caattggtgc aagcggtgca cgtatcttga ctacattagc ttacggattg
     1141 cttcgtgaac agaagagata cggtattgct tcattatgta ttggtggagg tttaggcttg
     1201 gcagtcttgt tagaagcgaa tatggagcaa acacataagg atgtgcaaaa aaaaaagttt
     1261 tatcagctta cgccatctga acggcgcagt caattaatag aaaaaaatgt attgacacaa
     1321 gaaactgcgt tgatttttca ggaacaaaca ctttctgaag agctctccga tcacatgatc
     1381 gaaaaccaag tgagtgaagt ggaaatccca atggggatcg cgcagaattt tcaaatcaat
     1441 ggcaagaaaa aatggattcc gatggcaaca gaggaaccat ctgtcattgc agctgcaagt
     1501 aatggtgcaa aaatctgtgg aaatatctgc gcagaaacac ctcagcggct gatgcgaggg
     1561 caaatcgttc tatcaggaaa atcggagtat caagcagtga tcaatgcagt taatcatcga
     1621 aaagaagagc tgattctttg tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc
     1681 ggtgttcaag atatctctac tcgggaattc atggggagtt tccatgcata cttatctatt
     1741 gattttttag tggatgtaaa agatgcaatg ggcgctaaca tgatcaactc gattcttgag
     1801 agtgtggcaa acaagttacg ggagtggttc cctgaagaag agattctatt cagtatttta
     1861 agtaattttg ctacagaatc attggcttct gcctgttgtg aaatcccatt cgaacgctta
     1921 gggagaaaca aagaaatcgg tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat
     1981 gcgaagcttg acccttaccg tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa
     2041 gctgtagtgg cagctacagg aaatgataca cgtgcggtta gcgcttcgat ccacgcctac
     2101 gcggcaagaa atggattata ccaaggatta acagactggc agatcaaagg ggataaacta
     2161 gttggaaaac taacggtgcc tttggctgtt gccacagtag gcggagcttc caatatcctg
     2221 ccaaaagcaa aagcatcttt agctatgtta gatattgatt cagccaagga actagcacaa
     2281 gtaattgctg cagtagggtt agctcaaaac ctggctgctt tgcgtgcatt agttacagaa
     2341 ggaatccaaa aaggccacat gggacttcaa gctcgttcat tagcgatctc aattggggct
     2401 attggtgagg aaatcgagca agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag
     2461 cagacagcta tacaaatatt ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga
     2521 agaataaagc aagatttaca gttattttat ttactgcaga tcttgctttt ttctctatca
     2581 gaaaatatgt ttattattag aattttttat agttaaggtt aggcatctta agaaatcagg
     2641 caactccaag atatcttcag gtagaagaga agtcgcctaa cattgtagga tcataggtga
     2701 tttgttggac aggtcaagct gatatttgag cctgatgtta gaaggaattt ctttttactt
     2761 gctgtagaaa ttattatatg actaaaaagg ctgggatttt aaatcccagc cttttatgac
     2821 aaatagaatt tttatcgatc agtaatcgct gtcgctgcaa attgttcgat caaaggaatc
     2881 aaagtcaatt tgactttgta tttccgttcg agtgcttttt cgataacgaa ctcattttca
     2941 gtagtcatag aaatagttgc taatttacga ggttcatcag catttttttc attaataact
     3001 acttcatgtt cattttgttc tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa
     3061 tcagcaaaac ttaaagcggc attcgtatca gttgagcgtg tattttcagt tgtgtccgct
     3121 tttggttttt ccgttttttc ttctggcaca ctgactgttt cttctgtaac tggtgtcgca
     3181 acagcttgcg cagttggttc atcgtttttc aatcgttgat cattccagta agacacgatt
     3241 tcaataagaa gattgtcacg gaatcgttcg aaaatcccgt tttctcctga acgaattctc
     3301 tttaatacag tattgaagtt gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt
     3361 ttcccagcta atccgatcgt taagatttga cggtttttgg gatcaagcat gacttgtaac
     3421 ggcaaaccat aacgtcgcaa gctctcttct tctagagaac ggaataattg tcctcttaca
     3481 ccatccaatg aaggacggaa ttgatttaat aatttttgtt tctgtttttt cttcatatgt
     3541 tctctccttc gctcataccc tttcgattat aagaaaaaaa cagccaaatg aaaaggagta
     3601 atgcaaaaaa acttgcgtat acgcaaatta caacagaaaa atttgcgtat tttcccgttt
     3661 tttgaaagaa aaataaa
//
LOCUS       DAJSVF010000162         3558 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSVF010000162 DAJSVF010000000
VERSION     DAJSVF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.171"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..2303)
                     /locus_tag="ON550_002834"
     CDS             complement(<1..2303)
                     /locus_tag="ON550_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955854.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ON550_002835"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ON550_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          995 a          790 c          561 g         1212 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacat ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaat
//
LOCUS       DAJSVF010000163         3505 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSVF010000163 DAJSVF010000000
VERSION     DAJSVF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.172"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(209..469)
                     /locus_tag="ON550_002836"
     CDS             complement(209..469)
                     /locus_tag="ON550_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955855.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            544..747
                     /locus_tag="ON550_002837"
     CDS             544..747
                     /locus_tag="ON550_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3955856.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(888..1373)
                     /gene="ssb"
                     /locus_tag="ON550_002838"
     CDS             complement(888..1373)
                     /gene="ssb"
                     /locus_tag="ON550_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3955857.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1376..2275)
                     /locus_tag="ON550_002839"
     CDS             complement(1376..2275)
                     /locus_tag="ON550_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3955858.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2288..2881)
                     /locus_tag="ON550_002840"
     CDS             complement(2288..2881)
                     /locus_tag="ON550_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3955859.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2957..3106)
                     /locus_tag="ON550_002841"
     CDS             complement(2957..3106)
                     /locus_tag="ON550_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955860.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(3122..>3505)
                     /locus_tag="ON550_002842"
     CDS             complement(3122..>3505)
                     /locus_tag="ON550_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCR3955861.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQN
                     VAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKE
                     DFTTGFEFSEKKKTNYKKRTRRTTK"
BASE COUNT          917 a          682 c          587 g         1319 t
ORIGIN      
        1 tttttctatt ttgtcttgct ttgcaagaca ccatatctta gtgaacgtgc aacgttcact
       61 tcctttcttc tcgaacggcg gtggcggttg gagtgagaga aaggtttttc aattgagaag
      121 gcacttgtgt cttcctaaaa aggtcttttc ctgtttctat gtaaaacaaa aagagctgca
      181 atcctcattt aggactgcag ctcttctttt atatgcccat cgatcgcgta aaatgatcgg
      241 ggtttctaat ttgcatcgat tcaatgacta attgattccg tttattgaaa tgacctgtga
      301 cggcaatatt gtattttcca tcatccataa ttaacaattt atcgataatt tcaatatctg
      361 caactacaca attaatcgat tcatgaattg tttgtaaagt aaagcgaacc aataatggtt
      421 gtgtttttat tactcggatt ttatcaagca aaccaacata tttgttcatc acgatcacct
      481 cgctaaaatt atagcaaaaa cagctaaaat tgagaatttt tattcgattt tagatatgct
      541 ataatgatca agacagtaaa gtttgtgacg gtggctatcc gattgaaagt gaggtggtgc
      601 gtatgtgcgt acttccaaaa tcttacagaa aggagaagcc ttttgcctgt tgaaacggcg
      661 ctaggattaa tgattagttt cggttctttg atcgcaacaa tcatttttgg aatcttagca
      721 gttgtaaaca acaatcaaaa aaaataaccg tcagctttag ccggtcgacg attatttttc
      781 aaaaataata ttaagctggt caccgtctta aacggtgctg ttaggggagt tgttagcgca
      841 actcctcttt tcgtatccat tataacaact ttattgatct tttacaatca gaatggtaaa
      901 tcatcatcac taatatcgat tgattgtccg ccaaatggat tttgtgaagc gttcaatcca
      961 ttgttgttag gcgtagctac atcccgttgt tcaaaggtgg tagggcttgg actgctggac
     1021 gattgatcag cgtgcgcacg gctttctgtc gctgctttac tttctaatag ttggaaatta
     1081 tcggccacaa cttctgtcac atacacacgt tgtccttgtt ggttatcgta ggttcgggtt
     1141 tgaatccgtc caacaacgcc gatcaaactg ccctttttag ctaatgtcgc catcgtttct
     1201 gctggtttgc gccaaatcac acagttaata aaatcagctt ctcgattgcc gttttgattc
     1261 gtgaaatttc ggttgacagc tagggtaaaa gtcgctactg cagtaccact cgcggtataa
     1321 cgtaaatctg gatctttggt taagcgtcct actaaagtta cattattgat catctttatc
     1381 tctccatttc ttgattcatt tgttgtgatc ttttaaactg ccgttttctt tcttgatttt
     1441 caattttgat cacacttttc attaggtgat caatttcgtt gactgtatct gtaaatgaaa
     1501 tggaattcgc aaacgtgtta actgagcgat atttttccca ctgcttttcc aaatagtcat
     1561 ttcctctgat cttatccatt tctctttcat aataaaagga caacggtttt gtatccccac
     1621 gttttgtaaa tgtatttcga acacttttgt ataactcttt cgtatcgata ttttctagtt
     1681 tcggaatggt aaataagtcg tagtaatctt ttgctctcga attatctgtg ccaaagctga
     1741 acgtagcact tagcttttct gcaataattt gttctgttgg ataagctgga atctccactt
     1801 ttttatcttc aaacattaat tggtgatagt gtgtatctac ttcaggataa atagaatctc
     1861 ctgttgtcac gtccatttta aacggtacac gtactttctc taaattagcc accaatttaa
     1921 tttgaaatcc aggataaaaa tcggcttctc ttgtttcctt cattccttga atagagaaaa
     1981 caatgccttc ttttgtgggt tttctagtta aatcattaaa aatgctcttc aatttttctt
     2041 cagttaattt gctatttcta aatgtcgtat cgatatcaat tgttgtcctt ctatcgattc
     2101 catattttga tcctaataaa aagccgcctt tcaaaatgaa gtcatttttg tactttgatt
     2161 cagaaatttg ttcaattaga cgatccatca taaaatgccg ttgcactaac tgtgcattta
     2221 atcctgtact ctttgctaaa tttcttgttt ttgctgcaaa ttgtgtcggt gtcatctttt
     2281 tcctccatca atataagctt tgcatgtggt atttcatttc tttttctaca tgtagttttg
     2341 tcatgtaatc tcttaattta cttggatctc tcaatgggtc ttccatgtac tcttttaatg
     2401 cttctaattt catttcatat tcaaattcat atctcggatt ccaaatatca caaagtgtac
     2461 gctctttatc atacatagta ataggatttc cttgaaagga agttccctct acttttccta
     2521 gttcataaac ttctttcttc acgaaatgaa attgtaactg ctgctctttt gtaattgaac
     2581 gatcaacgtt atagccatgg ggaaaagtta agtgaatgaa tttcggcata ttcgtagaaa
     2641 gctggtaaat atctaaagtc gtttcatgag agaagattcc cctggaaaat cgttgctgaa
     2701 gccaaaataa atcatctatt ccatgaccat cctctacata ataaccacga tcaactttga
     2761 tcaattttcc ttgattcacc aatctttgta cagtggttgc tgcgattcca aatttcctgg
     2821 cttccttcat agataaattt ccatcgcgaa tagccaacaa ttctttcagt ttatcttgca
     2881 tttacttccc ctcccttcgt tacaaataca ctacatcaaa tatatcatag tagtttattt
     2941 gtaacaagat taacgtctat cgatctaatt cgatttcttt ttgaatgcgc tgctggttct
     3001 ttaattgttg cagtcttctt ttatttgcac gtaatttctc ttgttctaaa cgaatttttg
     3061 ctttttgatt ttcgatttct tgttgtttgt gtgttgtatt tcgcatagga taaccccttc
     3121 attattttgt agtgcggcgt gttcttttct tatagttcgt ttttttcttt tcagaaaatt
     3181 caaagcctgt tgtaaagtct tcttttaaaa cgatcgttgc ttcaaacgaa cctttctgac
     3241 cttttagtcc tctgactgat tggcttgttc ggccgttttg aatgatctct ttcagttgtg
     3301 tggctgtgag tttctttttg gcaatcgttg tccataattt aaaatcacag gcttcagacg
     3361 tacatgccgc cacattttga taaagcaaga cttcttgttc ccgacatttt ggacaaacac
     3421 ctaaatgacg cgttgcttcc tgtttttcac tggctgcttt cacctcttgt aacgttgatc
     3481 caaaatctgt tgactggatt tgtcc
//
LOCUS       DAJSVF010000164         3493 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSVF010000164 DAJSVF010000000
VERSION     DAJSVF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.173"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(727..1767)
                     /locus_tag="ON550_002843"
     CDS             complement(727..1767)
                     /locus_tag="ON550_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3955862.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2116..2442)
                     /locus_tag="ON550_002844"
     CDS             complement(2116..2442)
                     /locus_tag="ON550_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955863.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2429..3232)
                     /locus_tag="ON550_002845"
     CDS             complement(2429..3232)
                     /locus_tag="ON550_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3955864.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1070 a          582 c          498 g         1343 t
ORIGIN      
        1 caaacatgcg ttccctctaa tttcagcgtt cgtcatacgg caaagagccc cacggacatt
       61 tttataacat tatgcttagg catagattca tcgtaatatc acgtattttt aaccttaaca
      121 cagccaaaaa aagtggtatc acttttgaat atctgttata ctaaatccgt tacgggtaat
      181 accgtaacac tgaaaggtaa acgcattaag gggtatagac ttaatgccac ctgtggacgt
      241 cgacgaaggt taccagcctt ccgagtctca cagggccttt cttttttttg tattttcaat
      301 tgtccaaatt tatcgtccaa aagtgcagaa cgaaaatcaa ttcatcacaa gcatagcaga
      361 caattaaatt taaaacaact aaaaatatac aaaaaagtgt aattgaatat tagcagcact
      421 gcctgaaaag aagtaatatc aggcttttca gcttggggaa ataagtgaat tacctctctt
      481 catgaattat tataactcaa aaaagcacat atgcaaacac tagttcccta tttttttgag
      541 tgaaaaataa ctaaaatatc taacgattat gaatttaaaa cctacgattg tgattgtttt
      601 cataaacttt taatctaaaa actccggaat actaactatt tatttgtaat atttgtaata
      661 aaattaaaat cataaaaaag ttctaattta aaattgatca tttttatagt aattgctttt
      721 tatcaactac tctaaccaat tttccgtact aactgttggt aattcgttaa atgtttctct
      781 ttgttcgttt gcaagctttg ccgcaatagc tttttcgaca aaccaatttt ttacataaac
      841 gaaaattaga ttctgcaaat tgtttacttt ttcagttttt tgtttttgat aagctttcag
      901 taaagtcata tataatcctt tgtcagcatc aattccataa gattccagtt cttcataaac
      961 gacaatagtt tgctctaatt cttcagcttt tttctttgca ttatgaatag tttggacggt
     1021 ttgttgagct tcactgtaac ttgaagaaaa tgtttttatc aagtttaata cattttctgg
     1081 tacaaatgta tttatactgt cctcttgcat gatgtcgacg aagttatcta gcaaaacttg
     1141 attttgtaac tggtcagttt cagtgtctat tatgtgtcta ttagtgtcta tagaattaga
     1201 atagaataga ttgtctgcga ttttcgcaat tacggttgag tccttgatat gactggattt
     1261 tgccgtattt gcgattttcg caattacggt agaatcattg atttttcgcg gttttgccgt
     1321 atttgcgatt ttcgcaatta cggtggaatc agtattgtct tcctcttcca tgattttaaa
     1381 gacgtcttga ttcgtaactt ctggcttgcc taagtacaat aaattaggca tagattcaat
     1441 cttaccatca cgtttattta ctcttcctct cttctgtttc agaagacctg ctttttctaa
     1501 ttcttttttg atcttagata ctttaccttc gcggcattgt aacaatttca ttaattcttc
     1561 aactgtgaat atgaagaata tattatcatc agaatctacc cagttatttt tgactgaata
     1621 atcaaatcga tctttaagta gcatataagc aatttttgca tcatttgaaa gatccatgta
     1681 tttttcgtta gtaaaaaata ccttaggcat ttgataatat ttttcacgat aaatttcttt
     1741 tgatttgtaa taattaaaat cattcatagc caatcctctt tctgtaggat agcgttttgt
     1801 tcaaaactat gttacaatga attaaacaaa tatatctatc cactatatat ttttctactg
     1861 ctgataagtc gccaaacttt gttaaagcag tagtcttttt ttatcttttt ttagtttacc
     1921 ttaaaaaata aattaaagca aaccgctcta tttcagggtt taagctcatg gtaactatat
     1981 cacgatatta caataacatt atatcacgat atcgtaatat tacaatatga agataagcga
     2041 cttatcagct ttgatactac tgatttttat aaaaaaaata tcacgatatt atgatatcgt
     2101 gatattgaat gtaaattaaa ttttattttg tttctttgct agtttatatc cttcaatcaa
     2161 gtattcttca ggtatggatt ttaatagcgc atcttctata acgtctggtt tacttttgtc
     2221 tgattcagca atagtcgtcc aatatttgag tgctgacatt agaattggat ttatacgaat
     2281 gctatctgct gttcttacac tacttccttg atcttttatg actttactta tatttttctt
     2341 ggttaattcg atttcttcta cttcttttgg tttgtagttc tttttatttt tatttcctag
     2401 tagtctactt actgcagctt gactgcgatt atcttctgcc atcaaaatca ccaacttctt
     2461 ttaaatattc ttctgtaaat gctttatagt catcttcagc tgttgagggt ttattaaaat
     2521 tgattagatc tgatccagca gcttgcgctt cgtcaacggt tattgttcta cgaatataag
     2581 ttttgaaaat ttttgcacca atgactctag aaatttcggt tgctgcagtc gtcatttctt
     2641 tagaaatgtt ataacgctta ttatacttag taaataggac tcctcgtact tcaagcttgt
     2701 cgttcgtata ttcttttacc gtttcaatag tttcacttaa ctttattagt ccttttaatg
     2761 agaaatacgc agcttcaact ggaatgataa tttcatcaga aacagtaaat gcgttgactg
     2821 taagcattcc taaacttggt ggacaatcaa ttataatata atcatagttg tccttgattg
     2881 gttgaagtac tcttttaagc ctgtgttcac ttcctacttg agtaaattcc ctctctgcag
     2941 aagaaagggc tatgtcggct ggcaaaacat caattccttt atagtccaga atagcatctg
     3001 taatgtcagc ctcccctttt aatacttcat aaattgtagg ttggtttgtt ccatctatat
     3061 cgaaacctac attatctgta gcatttccct gaggatcaat gtccactaaa agtacatttt
     3121 taccgtaaac aaatttcaaa catgctgcta aattaacgct agtcgtagtt ttagaaactc
     3181 cacctttttg attggctact gacaatattc ttgccattaa aatatccccc attcatctta
     3241 tattataata taattatatc ataatattgt gatattgcaa tatcgtatat gcattatatt
     3301 gtaatattgt gtgattattt tgttttaaag atatatatcg gttcggatat aaagattatt
     3361 catatgttat tcgaaagtaa aataatcact ttttattact ttaaaatttt ttattaatca
     3421 taagaaatat gttttgcgag gagagtgtaa aatattttgt gtaaatgaaa aaatccatac
     3481 aaaaaaggaa gtc
//
LOCUS       DAJSVF010000165         3470 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSVF010000165 DAJSVF010000000
VERSION     DAJSVF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.174"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..384
                     /locus_tag="ON550_002846"
     CDS             <1..384
                     /locus_tag="ON550_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCR3955865.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYHK
                     AAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKE
                     DFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            400..549
                     /locus_tag="ON550_002847"
     CDS             400..549
                     /locus_tag="ON550_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955866.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            630..1226
                     /locus_tag="ON550_002848"
     CDS             630..1226
                     /locus_tag="ON550_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3955867.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1239..2138
                     /locus_tag="ON550_002849"
     CDS             1239..2138
                     /locus_tag="ON550_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3955868.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2141..2626
                     /gene="ssb"
                     /locus_tag="ON550_002850"
     CDS             2141..2626
                     /gene="ssb"
                     /locus_tag="ON550_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3955869.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2992..3252
                     /locus_tag="ON550_002851"
     CDS             2992..3252
                     /locus_tag="ON550_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955870.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         1256 a          599 c          712 g          903 t
ORIGIN      
        1 ggacaaatcc aatccactga ttttggatcg acgttacaag aggtgaaagc agccagtgaa
       61 aagcaagaag ccgcgcgtca tttagggatt tgtccaaaat gccaggaaca agaagtttta
      121 ctctatcata aggcggccgc atgtacgtct gaagcttgtg attttagatt atggacaacg
      181 attgccaaaa agaaactcac agcgacacag ctgaaggaaa tcattcaaaa tggccgaaca
      241 agccagccag tgaaaggatt aaaaggtcaa aagggttcgt ttgaagcaac gattgttcta
      301 aaagaagact tcacaacgag ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa
      361 agaacacgcc gcactacaaa ataatggagg ggttatctta tgcgaaatac aacacacaaa
      421 caacaagaaa tcgaaaatca aaaagcaaaa attcgttcag aacaagagaa attacgtgcc
      481 agtaaaagaa gacttcagca attaaagaac cagcaacgga ttcaaaaaga aattgaatta
      541 gatcgataaa atcttcctgg ctattgtata cgcataaact tatgttatac tatatttata
      601 agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtttac aagctgctag
      661 aagaatttaa cggaactttg gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa
      721 cgatcaaacg tatggtagat cgtggagagc tggatcgtga gtatccaggt tttttcacct
      781 tacctggtca atttcctgat gaattgttta tggcacaaaa gaaatatgag cgcggcatta
      841 tttcgcatat caccgcgttg gatttatatg atttaacgga tatgatccct agacagatcg
      901 atctaaccgt accttacggt tatcacgttt ctgaaaaggg gttgaaagaa ttcgcagttg
      961 agttacacta ttcaaaagcg gaatggtatg aattaggtaa gatcgaaata aagagtcgtt
     1021 atgggaatcc tgttatagct tatgatccag aaagaacctt gtgtgacatt tggaatccct
     1081 ggtacaacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg gagtcggaca
     1141 gaaaaaatct tagaaaattg aatgagtatc gacgaatttt gcctacagac aaaacgatac
     1201 gctcttatat tatggcttta aattaaagga gaagacacat gacacctacg caattaaaag
     1261 caaaagtgaa gaatatttcc agagaaaaag aagttgatcc gcagcttgtt ttaagacatt
     1321 ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa aattttgtgt
     1381 taaaaggcgg cttcttaatt ggctcaaagt atggcattga aaatcgaaca acaaaagaca
     1441 ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attgacaacg gttctaaacg
     1501 atattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga atgaaagaaa
     1561 caagggaagc tgattattat ccagggttta gcttacgtgt actggcacat ttggaaaaca
     1621 tgcggccaga ttttaaggta gacgtgacga caggtgactc gatctatcct gcaacgatca
     1681 ctcacagtca taaattgatg tttgaaaatc gaacgattcc tttggaatct tatcctacag
     1741 aacaaattat tgccgaaaaa ttaagtgcaa cctttgattt tttaacagat aattcgagag
     1801 gaaaagattt ttatgatctc tatactattc ctaaaatgga aaagatcgat gagaagacct
     1861 tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg ttaaagattt
     1921 attacgaaaa ggatatggat gtactgaaaa atagtcatga gctaaaagat acctggaaga
     1981 aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgaagagaca atggattcga
     2041 ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa atagcttatc
     2101 aaagacacca acagatgaac agagaaatgg agagataaaa atgatcaata atgtaacttt
     2161 agtaggacgg ttaaccaaag atccagattt acgttatacc gcaagtggca cagcagtggc
     2221 tacttttacc ctagccgtta atcgtaattt cacgaatcaa aacggcaatc gagaagctga
     2281 ttttatcaat tgcgtgattt ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa
     2341 aggcatcttg atcggcgttg tcggacggat tcaaacacgg acttatgaca accagcaagg
     2401 acaacgtgtg tatgtgacag aagttgtagc tgataacttc caactattag aaagcaaagc
     2461 agcgacagag agccgtgcgc acgctgatca atcgtccacg agtccaagca ccaccacttt
     2521 tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac agaatccatt
     2581 tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttaagtag cattttctat
     2641 tggttgcatt tgctacggtt tatgttataa tttagtcaac aaaaagaatt ttcaatctat
     2701 aaccaaaaac cactagctgg cgggctagtg gttttctttt acgtttctat tataagaatt
     2761 aacgagtagg cttactgtct ttcttggcaa atttacaatc aagccatttg cagatatagt
     2821 gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc aaacctataa
     2881 cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta tagcataatt
     2941 gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt gatgaacaaa
     3001 tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt ggttcgcttt
     3061 actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga aattatcgat
     3121 aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca cttcaataaa
     3181 cgaaatcagc tggttattgc tagcatgtat gttagaaatc ccgatcattt tacacgatcg
     3241 atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc tctttttgtt
     3301 ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc cttctcaatt
     3361 aaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgct
     3421 gcacgttcac taagatatgg tgtcttgcaa agcaagacaa aataggaaaa
//
LOCUS       DAJSVF010000166         3400 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSVF010000166 DAJSVF010000000
VERSION     DAJSVF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.175"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..278
                     /locus_tag="ON550_002852"
     CDS             <1..278
                     /locus_tag="ON550_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HCR3955871.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="ON550_002853"
     CDS             421..642
                     /locus_tag="ON550_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3955872.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="ON550_002854"
     CDS             658..1500
                     /locus_tag="ON550_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955873.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="ON550_002855"
     CDS             1563..3365
                     /locus_tag="ON550_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR3955874.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1231 a          468 c          702 g          999 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcctat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata
//
LOCUS       DAJSVF010000167         3338 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSVF010000167 DAJSVF010000000
VERSION     DAJSVF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.176"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..237
                     /locus_tag="ON550_002856"
     CDS             <1..237
                     /locus_tag="ON550_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955875.1"
                     /translation="TENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKE
                     KRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            247..450
                     /locus_tag="ON550_002857"
     CDS             247..450
                     /locus_tag="ON550_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955876.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            661..1245
                     /locus_tag="ON550_002858"
     CDS             661..1245
                     /locus_tag="ON550_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955877.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1616..2941
                     /locus_tag="ON550_002859"
     CDS             1616..2941
                     /locus_tag="ON550_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3955878.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2934..3284
                     /locus_tag="ON550_002860"
     CDS             2934..3284
                     /locus_tag="ON550_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955879.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1190 a          495 c          666 g          987 t
ORIGIN      
        1 actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga
       61 gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg
      121 aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat
      181 aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga
      241 ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt
      301 aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca
      361 caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga
      421 gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca
      481 ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac ttcctttttt
      541 tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg
      601 ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt
      661 ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac
      721 agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt
      781 aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt
      841 tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg
      901 aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt
      961 tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg
     1021 atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt
     1081 cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt ggatagcggt
     1141 acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat
     1201 ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat
     1261 gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt
     1321 gtctactgat aacttattat caaatgttgt aaggatttta ttcatttgtt aatattaaag
     1381 aacggaactc caaaaaagtc cgtactctaa tttgcagtct ctcactcttt tagagtgaga
     1441 gtttttatta gattttcaaa ttaattagta aagtgataga ataaattgat catacaaatc
     1501 ataagaagaa gcagggtgat agcataccta tgcttctttt tttatgtatt tagctatttc
     1561 ttctttacaa catcgaacat atgtttgtat aatgtggaag aatggagtgt tatttatgat
     1621 ggattattca aaagagccag taaatgatta ctttttaatc gatatgaaaa gcttttacgc
     1681 aagtgtcgaa tgtatagaaa gaaatttaga tccattaaca acagaacttg ttgttatgag
     1741 tcgatctgat aatactggtt caggattgat attagcttct tctcctgaag caaaaaagcg
     1801 gtatggtatt acaaatgtga gtagaccacg tgacttacca caaccatttc ctaaaacact
     1861 acacgttgtt ccaccacgta tgaacctata tatcaagcga aatatgcagg taaataatat
     1921 tttcagaaga tatgtggctg atgaagatct actgatttac tcgatcgatg aatcgatttt
     1981 aaaagtgaca aaatcactga atctttttac gactgaagaa acacgaagtc agcgaaggaa
     2041 gaagctcgct caaatgatcc aagaacgtat taaagaagag ttaggtttga ttgctacggt
     2101 aggcgtcggc gataatcccc ttttggctaa attagcctta gacaatgaag caaagcataa
     2161 tgaaggattt attgcggaat ggacgtacga aaacgttcct gagaaagtat ggaatattcc
     2221 cgaaatgacg gacttttggg gaattggttc tcgaatgaaa aaaaggctca accaaatggg
     2281 aattttgagc attagagacc ttgctaactg gaatccttat acgattaaga atagattagg
     2341 tgtgattgga ttacagctgt acttccatgc taacggaatc gatcgaacag atatagcaat
     2401 cccgccagaa ccgacaaagg aaaaatctta tggtaattca caagtgttac cacgagatta
     2461 tactagaaga aatgaaatcg agctggtggt aaaagaaatg gcggaacaag tagccattcg
     2521 tatcaggcag cataattgta aaacaggatg tgttcattta aacattggta cgtcgatctt
     2581 agaaacgaga ccaggatttt cacaccagat gaaaatacct atcacagata atacaaagga
     2641 attgcagaat tattgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca
     2701 cgttggaata acctatagca agcttgtgta caccgattcg cttcagctgg atttatttag
     2761 tgatccacaa aagcaaatta acgaagaaaa tttagataaa attattgaca agattcggca
     2821 aaaatatggt tttacgtcga ttgtacatgc ttcttcaatg ttagaaagcg ctagaagcat
     2881 tactagaagc acacttgttg gtggtcatgc gggcggaaat gggggaatta aaaatgattg
     2941 attacaatga tcgtggacgt ttaaagtggg cagggtttta tttatctgat catactgaag
     3001 aaattgatgc cgacaatgaa caacgaacca gtcaaaattt agcaaaagaa caaatgacga
     3061 ctgaagagat tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatctattc
     3121 aaaaagaaga acgaaacgct gaaggatact atccagaaga tattgttggc aagatcttag
     3181 gtagtgatga gctggggatc tacgttggaa aagacaaaat tcactacgac gaaatacgtc
     3241 atatcgaatt ttaccaggat aataaatgga gtcgattaga atgaacatta tccagcaata
     3301 tgagctaaag tatataacct ttgatcagct atcagaag
//
LOCUS       DAJSVF010000168         3218 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSVF010000168 DAJSVF010000000
VERSION     DAJSVF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.177"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1050
                     /locus_tag="ON550_002861"
     CDS             <1..1050
                     /locus_tag="ON550_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955880.1"
                     /translation="KIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSN
                     YLINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVW
                     SKTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMR
                     DSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDT
                     TAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYF
                     VALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRN
                     SSYGSDYTFAITYTI"
     gene            1067..1216
                     /locus_tag="ON550_002862"
     CDS             1067..1216
                     /locus_tag="ON550_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955881.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1213..1659
                     /locus_tag="ON550_002863"
     CDS             1213..1659
                     /locus_tag="ON550_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955882.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1661..1798
                     /locus_tag="ON550_002864"
     CDS             1661..1798
                     /locus_tag="ON550_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3955883.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1836..2129
                     /locus_tag="ON550_002865"
     CDS             1836..2129
                     /locus_tag="ON550_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955884.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2126..2350
                     /locus_tag="ON550_002866"
     CDS             2126..2350
                     /locus_tag="ON550_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3955885.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2347..>3218
                     /locus_tag="ON550_002867"
     CDS             2347..>3218
                     /locus_tag="ON550_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3955886.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSG"
BASE COUNT         1143 a          548 c          667 g          860 t
ORIGIN      
        1 aaaatcgaag aaggctcaac agccacaccg taccagccta atctacttga tgcaccgtat
       61 tatttgagta aggtggcttt gggtgagaat ctaattaaac cagaatcaca acaaccagtt
      121 actaatagta actatcttat taacacctat aacattaaac caatggtaaa aggtaagaag
      181 tataccatca cacttgaagg aactaagcca gcaacacagg tttttagacc atttttcaca
      241 cgagcaacag gagatgcatg ggaggttggt gacttacaac cagtagaagg cttaactaat
      301 gtgtggtcta aaacatttac agcagcagat gactcacacc ctactacccc acaagtgcag
      361 atttatcaag taccaagcac aagtgtagga caatgtacaa ttaagtggtt aaaactagag
      421 gaaggcaata ctcgaacccc gaatattagt gagtataaat atcgtggtac tggtatgcgt
      481 gattcaaaca atccaaaaga ttatgtttgg gatctagcac cagaatatgt cgaagataat
      541 ttggccacag atattaaaat ttctgaaatt actggtaaag caaacaatta taccgatggg
      601 aaagtatcgg agattaattc gcagttgact gcttcaatta atgaagtaga caccacagct
      661 aaggatgctc aaacaaaagc gaatgctaat gcgactgcta tagatgaatt agacaataag
      721 atcgatgaac gcattaatga tacagctact accacattaa cagttacaaa cgggaatacc
      781 ggatcagcaa agctttatcg tgaaggaaaa acagtttcta tatattttgt ggctttaaac
      841 ggaaaacgca gtggtggaaa tgattcaacg atactaacaa ttccagaagg ctatcggcca
      901 ccaattagtt ttgagcaact ggttggctcg atagaccgtt ctactttgaa cagtgctcag
      961 ttatctattg gtgcagatgg agccattaaa tggcgaagaa actcaagtta tggatcggat
     1021 tatacctttg caattactta cacgatttag aaaagcgtga atcgatatga aggcagcata
     1081 tagaccaatt gaaccttacg gattcgagca aatcattgtg aatgatgaag aacatttacc
     1141 ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg
     1201 ggagggaaat aaatgaaaaa catttggaaa tatggacgta ctggcggaga gtacgcagga
     1261 aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc gcttgaagga
     1321 attcgtgctg atggcgaacc gttaacaatc gctgatcaaa tgtttgatcc taaattgaat
     1381 caatggatta ttttagcgaa cgcactagat cacaacgatt taaacaatct caaagcaatg
     1441 tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga tcaatgccaa actcatgcta
     1501 agcgatgtag caattaaaca ggaaaatact gcattgaaag aaaaagcgga tagtttagca
     1561 caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt
     1621 aaagagcaac taaatccagc ttcaaaggga ggtgagtagt atgtttagtt ttagcgatgt
     1681 gaaaatgatg tatgattggg gctgtttcac taacgaacaa gtaatggttt tcgttccgtt
     1741 gtgcattact gaagaaaaag cagataaaat cattagcaaa gaagagagcg catcttaatt
     1801 gatgtgcttt ttattttgat tcaaggagtt gtcacatgat taatttaggg gaatggggag
     1861 cgatagcagg atcaataacc gctatcgttt ctttgatttt attagtaata aaaccaatta
     1921 ctgcatcttt ctcgaagatt actgagactc tttcaaaagt aagtcacaat ttagatttgc
     1981 tgactaaaga tttagaatcg agcaaatcag atcgattgat gattcatgaa gaactaaaga
     2041 aacacgatga aagattagat acacatgcag aaaaattggt agaacacacg caacaaatta
     2101 aaactttatt tagggagaga agaaaatgaa taataaaacg ttcgaagtac taaaatggtt
     2161 cgcactggta attattcccg cactagctac tttcgtgggg ttagttggta aagcgctcaa
     2221 ttggcagtac acagatatct gtgttgtcat cattactggt tttggcgcgt ttttagggag
     2281 tgtgttgggt gtatcaaatc gaacctacaa aatgttctcg gctgaaagcg aagaaggagg
     2341 aaacaaatga aaaagaaaat tactattact gcgatgagcc tgttaacggc tcttttttta
     2401 ttacccatta atacgtttgc ttatactatt aatgacgagt ataatttagc gccgaatcaa
     2461 ggagactcca gattagcaat tcctaacaaa attattttgc acgaaactgg aatagatgca
     2521 ccagcaagaa acgtagccgc caacatgaaa aataattata acggaagcaa ttcttatact
     2581 acagatgtta ttggtgacgg tgggattgtt taccgtgtgg gtgagcaagg atatgtttcg
     2641 tggggagctg gtaacgctaa tccttatgcg cctgtacaga ttgaattaca gcgcacatat
     2701 gataaagcat tgtttgaaaa aaactatcga gcttatattg aatatacaag agatagtgca
     2761 aaaaaatatg gaattccatt gactcttgat caaggaactt ctttatttac aaaaggaatc
     2821 atttctcatt tgtgggtgac aaattatgtt tgggggaacc acacagatcc atatggttac
     2881 ttatcgcaaa tgggagttag caaagaaaag cttgcttatg atttagctca tggatttacc
     2941 gatgaaaatc caacaacttc tgaaaacaag cctgtcattg atccaacacg agctggtgca
     3001 gctaatccta cgctgacaga tggaacaaat cacgcccaca ttgatcagtt tggggaaatc
     3061 gaaaacgcaa acttgcatgt ggctggatgg cacattgcta actataaata cgagtatatt
     3121 ttcattatgg actacaatac tgggaaagaa ttagctcgag taagagctga tggaatttat
     3181 agaccagatg taaaccaagc ttataatact tctggaaa
//
LOCUS       DAJSVF010000169         3213 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSVF010000169 DAJSVF010000000
VERSION     DAJSVF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.178"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..114
                     /locus_tag="ON550_002868"
     CDS             <1..114
                     /locus_tag="ON550_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR3955887.1"
                     /translation="LEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            244..705
                     /locus_tag="ON550_002869"
     CDS             244..705
                     /locus_tag="ON550_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HCR3955888.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            739..1374
                     /locus_tag="ON550_002870"
     CDS             739..1374
                     /locus_tag="ON550_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCR3955889.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            1487..2122
                     /locus_tag="ON550_002871"
     CDS             1487..2122
                     /locus_tag="ON550_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955890.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            2421..>3213
                     /locus_tag="ON550_002872"
     CDS             2421..>3213
                     /locus_tag="ON550_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3955891.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKI"
BASE COUNT         1297 a          467 c          539 g          910 t
ORIGIN      
        1 ctcgaaaaat ttatggagta ttattctatt cagaataagc tcagatacac ttgttatgct
       61 gtctgccaat gctattttaa gaaaaaatat tcttacgcaa ggcaatatgt atgaatacat
      121 caagaattat taaaaaaacc gatttcagca aaacattaaa aaagcccgtg ctgcaacacg
      181 ggctctttcc tcatatatga gcttctacaa aaaaaatcat atcacagaaa tgaggaagaa
      241 aaaatggaaa aagagatttt aataaaatct ccaggaaaaa ccttgattaa agtaactgat
      301 gattctattt caattattag aaaaggattt ataaatttag ttaatcaagg tattaaagga
      361 gaaaaaacta tcccctttaa aaatatatca gccgtacaat taaaaaaacc aggtatgagt
      421 aatggttata tacaatttac cttgctaggc ggaaacgaaa gtcgcggagg tatattagcg
      481 gccacgaaag atgaaaacac cgttatgttt acaaaaaaat attggaatga aatggaaaat
      541 ttaaaaaaat atatagagaa gcaacaatct atattagata ataattccaa agaaacacaa
      601 atttcaagtg cagatgaaat taaaaagtat aaagagttgt tagatgaagg gattattaat
      661 caagaagaat tcgattttaa gaaaaaagaa ttgcttggtt tataatattc aatatcaaaa
      721 gagaaagagg atctattaat ggacggtata tttggtttta ctggtattgt tttatttatt
      781 ataggtttaa tcatgttaat tgtaagattt atcaaaaaaa caaataagaa aacacctatg
      841 attctcctta ttatcgggat tatttttact accgtaggtt tctcgctttc ctcatcaaac
      901 gaggcaaatg acaataacaa acaggaaagc tcatcatctg atactcaaac tactacatct
      961 aacagcagca ataaaagcct aactcaattt gaagaatttt tagagacaaa taatcatgac
     1021 tggggtgctt tcttagatag ttattattct attactccag ctactgagca aaatacagct
     1081 tttactaaat atattagtgg taaaacatat acttttgaag gaactgttat cgaatcaatg
     1141 actactagaa tagccattat agcagacaaa gagtacgatg ataaaagttg gaatgatatt
     1201 tctgcaacac ctaaagtttc atatgtaatt tttgcgaagg acttaaacaa tgctgatacc
     1261 tttacaaagg gcgataaagt aacttttaca ggagaaatta gttcaagagg atcgaacata
     1321 gaaaaatcct atgctcaatg ggatatgatt aatagtaaag taagtaaaaa ataaatgaac
     1381 cacgccccac taagagttag attgtgcaca ataataaaac ctatacaaaa gagtgccatc
     1441 aggagtcaca atgcttgtaa aggaaatgac aaaataggag gtaactatgg ataatagcaa
     1501 tgaaaaacta accaaaaaag aatatcttga attaatagtt gaaggcggta ttggagcaat
     1561 tccttatatt ggtggcgttg tgcagtcact ttattttggg tctaaaaacg agaagcgttt
     1621 caaacgcatc gagaaattct acaaaaattt aaacgaatca ttagaaaaca ttcgcgatca
     1681 aattccagaa gatgttttca acttagagaa caaggaccaa ctcattggaa tattcgaagg
     1741 gataaatgac gaggtagaaa aatcaaaggc acaaaataaa atagatatgt ataaaaatct
     1801 ttataagaat tgtttacttc gaattaacag cgcttcatgg gataacgagg agtattatct
     1861 tcttgtactg aatcaattaa catcgattga gattcaattg ttagcatgct taatgagtcg
     1921 tggaaacgaa aatttcactg gaaatatcag catgtctggc tattcacaag agctaataga
     1981 tggtagctta aatagattat ctgattttgg tctgttagaa aaaacgatta gctccatagt
     2041 attaggtgat gtcggcaaac aaaatatggg ctacaaaata tctcatttag gaattcaatt
     2101 tattaactca actcttactt aagttttctt tctaatatga catctagacc tatcagcaat
     2161 gcaacaataa aaaaccagac ataccaataa tcaacaaata atctaaactc tggaagttca
     2221 gttattcttt taaaaatcac tttaatcata ataaacacct ctgattggat gatctaatac
     2281 acatccaatt ataccattaa aacgacaaat agcacatcct tgcgccggca agcaaaaagg
     2341 atgtgcaaaa aataataaaa cccaaacaat gggcttcttt atagttccta ttgtatcaga
     2401 gaaagagagc tgattcaatt atggcaagat tagtcaaacg tggaaatagt tggcaatacg
     2461 aaatttcata caaaaaagat gacggaaaat acacgaagat aagaaaatca ggatttaaga
     2521 caaaaggaga agcaaaagat gccgccaacg aattagaata taacttgaac aaaggcctta
     2581 aaggggatcg caaaaatcta ttattatcag attactttga ggattggatg caactttata
     2641 aagaaggaac agtatctcct atcacttata gaaaatacga agatacgtta atgaacataa
     2701 agaaatatat gccagcggta ttgatttctg atttagatag agtgggatat caacgctttt
     2761 taaataagta tgctaaagac catgtaaaat ctaccgttat taagtttaat aaccatatta
     2821 gagcgtcgtt gaaagatgcc gtagaagaag gattaattcc gtttgatcca actagaaaag
     2881 cagtaatcaa aggaaaagat tcattgaagc caaaagaaga taaatattta gattatgatc
     2941 aatttaaatc tttaatgaaa ctcgtagaag aaaacctttc tgcgcagtac tcttctccta
     3001 tgctcgtgtt agttgctggt gccactggaa tgcgatttgc tgaacttcta ggattaacat
     3061 gggaagatat cgatttcgaa gatcaaatca tcactattaa taaaacatgg aattataaat
     3121 taaatgaatg gggaaaaaca aaaaacgaaa cttcaaatag gaaaatttcc attgataaac
     3181 atacgattga tctcttaaaa aagtttaaaa tca
//
LOCUS       DAJSVF010000170         3199 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSVF010000170 DAJSVF010000000
VERSION     DAJSVF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.179"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            301..585
                     /locus_tag="ON550_002873"
     CDS             301..585
                     /locus_tag="ON550_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955892.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ON550_002874"
     CDS             588..1004
                     /locus_tag="ON550_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955893.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ON550_002875"
     CDS             complement(1141..1860)
                     /locus_tag="ON550_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3955894.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="ON550_002876"
     CDS             2513..2608
                     /locus_tag="ON550_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3955895.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="ON550_002877"
     CDS             complement(2910..3155)
                     /locus_tag="ON550_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955896.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAJSVF010000171         3190 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSVF010000171 DAJSVF010000000
VERSION     DAJSVF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.180"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            118..1857
                     /gene="yfcC"
                     /locus_tag="ON550_002878"
     CDS             118..1857
                     /gene="yfcC"
                     /locus_tag="ON550_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3955897.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2014..>3190
                     /gene="iadA"
                     /locus_tag="ON550_002879"
     CDS             2014..>3190
                     /gene="iadA"
                     /locus_tag="ON550_002879"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3955898.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
BASE COUNT          977 a          537 c          749 g          927 t
ORIGIN      
        1 aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg tataattcat
       61 ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg
      121 aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat
      181 acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca
      241 gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact
      301 cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg
      361 tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg
      421 gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta
      481 gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat
      541 acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt
      601 ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta
      661 gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga
      721 gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct
      781 ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag
      841 gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc
      901 gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc
      961 ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca
     1021 ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat
     1081 gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc
     1141 cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg
     1201 atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc
     1261 tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt
     1321 gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct
     1381 ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt
     1441 gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc
     1501 gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa
     1561 agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc
     1621 atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag
     1681 cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta
     1741 ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc
     1801 ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct gcagtagtac
     1861 tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat
     1921 tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat
     1981 catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg
     2041 tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct
     2101 gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag
     2161 ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga
     2221 gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt
     2281 gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg
     2341 atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg
     2401 taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat
     2461 aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt
     2521 gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct
     2581 gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc
     2641 gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca
     2701 gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct
     2761 agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg
     2821 aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa
     2881 ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc
     2941 aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc
     3001 gccttacgag ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa
     3061 atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat
     3121 acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact
     3181 ttcgaaaaaa
//
LOCUS       DAJSVF010000172         3080 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSVF010000172 DAJSVF010000000
VERSION     DAJSVF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.181"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            65..337
                     /locus_tag="ON550_002880"
     CDS             65..337
                     /locus_tag="ON550_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3955899.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            339..1202
                     /locus_tag="ON550_002881"
     CDS             339..1202
                     /locus_tag="ON550_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3955900.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELAKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGL"
     gene            1643..1960
                     /locus_tag="ON550_002882"
     CDS             1643..1960
                     /locus_tag="ON550_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955901.1"
                     /translation="MEKTKKLQLEDFTENEFYGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            2494..2991
                     /locus_tag="ON550_002883"
     CDS             2494..2991
                     /locus_tag="ON550_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3955902.1"
                     /translation="MKYIKNVETLEELKKAYKKLALKLHPDCGGNEEEMKILNNEYDE
                     LFSKLKNTHKNKEGETYTKETTETPEQFKDIINKLFNLKMDGVAIEVVGTFIWLTGNT
                     KPYKDDIKALEFRYSPKKYAWYKAPSDYKKRSRKNYDMDTIRGMYGSQKIKEEKEEKK
                     YLQAN"
BASE COUNT         1257 a          511 c          531 g          781 t
ORIGIN      
        1 aaagccttag aagaatatat tcggaaatat ttacctgaca aactttaaaa ggagtgaaaa
       61 tgaaatggca gttacatatg aaaaaacatt tgaaattgaa atcattaatg agttatcggc
      121 aagtgtttat aatcgagtat taaactatgt tttgaaccat gaattaaata aaaatgactc
      181 tcaattattg gaagtcaatt tattaaacca attaaagctt gcaaaacgtg taaatctttt
      241 tgattattct ttagaagaat tacaagccgt tcatgagtat tggcggtcaa tgaatcgtta
      301 ctcaaaacaa gttttgaata aagagaaagt ggcttaatat ggcaaatata gtcaatttta
      361 ctgacaaaca atttgagaat cgcttaaatg ataatttaga agaattgatt caaggaaaaa
      421 aagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca gggaaaacca
      481 gtttgcgatc agcaattctt gaagaaacac aagggaatgt tattgtcatt gataatgata
      541 cctttaaaca acagcaccct aattttgatg aactagcgaa actttatgag aaagacgtag
      601 taaaacacgt caccccttat tctaatcgca tgacagaagc gatcataagc cgtttgagcg
      661 atcaaggcta taatttagtg attgaaggta caggacgaac aacagacgtt cctattcaaa
      721 ccgcaacaat gcttcaagcc aaaggttatg aaacaaaaat gtatgttatg gcagtaccta
      781 aaatcaactc atatttagga acaattgaac gatatgaaac tatgtatgca gatgatccaa
      841 tgacagccag ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca
      901 atttagaaac ccttcataaa acgggcttat ttagcgatat aaggctttac aatagagaag
      961 gagtgaaact ctattcaagc ttagaaacgc cttctattag tccgaaagag accttagaaa
     1021 gagaattaaa tcgtaaagta tcagggaaag aaattcaacc gactttggag agaatagagc
     1081 aaaaaatgct tcaaaatcaa caccaagaaa cccctgaatt taaagcaatt caacaaaaaa
     1141 tggaaagctt gcagccacct acaccaccaa tacccaaaac acctaaactt ccaggacttt
     1201 aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
     1261 aacatttcct catgttttaa aagctgatcg agaaataaag cacacaacgg tattctttct
     1321 tttttcagtt ggtacatttt gcagtctgta acgtgtcatt tactgtttgt taccttaaca
     1381 tatccagtcg aaaacacaag ggtatataaa cgagcaagct cgcccttgtg ttttctttgt
     1441 gtctatgttg cgttctcgtc acagcaaacg acacgaacac actggcgaaa atgaaccaac
     1501 aaaacgaaaa aagaaaggag cgtacactgt aatttatttc tagcttttaa aacatgagga
     1561 aaacagcttc acttcctcaa acgcctaaca aaaatggttg tcaaaaaact aaaaatcaaa
     1621 aaaaagaaaa gaggaaaaga aaatggaaaa aacaaaaaaa ttacaattag aagattttac
     1681 agaaaatgag ttttatggaa cacaagaaca gcaatatcta aaagctcaag taagagaaga
     1741 gctaaaagag caaggcttta tcatagatag cagttttgag ggcgatttta aaacatggat
     1801 aggtgtatac gcaagaccaa aagataaacc gacctactta gaccctcaaa acgataaaga
     1861 agcagaagaa caagagcaat attctattaa cggctttaaa caagatttta gcgaatggtt
     1921 tgagtgggaa ataaaaaatt tgaaaattaa agaaatgtaa aagaaaagcc tagcaaaaaa
     1981 agctaggttt ttcttttaca taaaacacca aaaagaaaaa cctaagaagg ataaaataag
     2041 ttgttccact tgcggtgcag ttgctcctcg ttccaaaccc aacttatttc tttgcaaaga
     2101 tatttatatt tgactaacac ctttcctcga atggtaaaag ctaaccgttc gttactctac
     2161 ctcaaatact ttcttttttc agttggtaca ttttgcagtc tgtaacgtgt catttactgt
     2221 ttgttacctt aacatatcca gtcgaaaaca caagggtata taaacgagca agctcgccct
     2281 tgtgttttct ttgtgtctat gttgcgttct cgccacagca aacgaaacga acacactggc
     2341 gaaaatgaac cacctaaacg aaaaaagaaa ggagcaagat atgtagaaca ctcacagctt
     2401 ttaccattcg aggaaaacag cttcacttcc tcaaacgcct aacaaaaatg gttgtcaaaa
     2461 agccaaaaat caaaaagaaa aggaatgata aaaatgaaat acattaaaaa cgttgaaaca
     2521 ttggaagaat tgaaaaaagc ctacaaaaag ttagctttga agttacaccc cgattgtggc
     2581 gggaatgaag aagaaatgaa aattttgaac aatgaatatg acgaactgtt tagcaaactt
     2641 aaaaacactc ataagaataa agaaggtgaa acttacacca aagaaacaac agaaacaccc
     2701 gaacagttta aggatattat taataaattg ttcaatctaa aaatggacgg cgtagcaatc
     2761 gaagtagtag gaacatttat atggctaaca ggtaatacaa aaccttataa ggacgacata
     2821 aaagccctag aatttcgtta ttcaccaaaa aaatacgcat ggtataaagc accaagcgat
     2881 tataaaaaac gtagtcgaaa aaattatgat atggacacaa taaggggaat gtatggaagc
     2941 caaaaaatta aagaagaaaa agaagaaaaa aaatacttac aagccaatta aggcttgtaa
     3001 gtttatcaga aaggaagaaa ccattatgtt ttataaaata agaataattg aaactttgga
     3061 aaaggaattt gaaattgaag
//
LOCUS       DAJSVF010000173         3080 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSVF010000173 DAJSVF010000000
VERSION     DAJSVF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.182"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..147)
                     /locus_tag="ON550_002884"
     CDS             complement(<1..147)
                     /locus_tag="ON550_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3955903.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIR"
     gene            complement(162..521)
                     /locus_tag="ON550_002885"
     CDS             complement(162..521)
                     /locus_tag="ON550_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCR3955904.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(518..1369)
                     /locus_tag="ON550_002886"
     CDS             complement(518..1369)
                     /locus_tag="ON550_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3955905.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1386..2216)
                     /locus_tag="ON550_002887"
     CDS             complement(1386..2216)
                     /locus_tag="ON550_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR3955906.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2219..2905)
                     /locus_tag="ON550_002888"
     CDS             complement(2219..2905)
                     /locus_tag="ON550_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955907.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(2911..>3080)
                     /locus_tag="ON550_002889"
     CDS             complement(2911..>3080)
                     /locus_tag="ON550_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955908.1"
                     /translation="VANKYEKSIDEAIVALTDAASITADDSKWTKRDLGILKRGVNWL
                     EDQYREETKEK"
BASE COUNT          746 a          661 c          488 g         1185 t
ORIGIN      
        1 acgaatttcc cttcttaagt tctctatgtg caaatcgatt gcctttctcg ccgtttcatt
       61 gaccatcact gcctttgttc gttctagatc gtcaatttca cgctgaatgt ttcgaatacg
      121 catttgaatc acttcttctg ttgtcatgat ggaccacctc gttaaaaacg ctcttccttg
      181 aacgtattcc gatatttttt agctaatatc aacggcgctt gatattgatg acagaacaac
      241 tttgccttga tcttaaagtc ttttgtctgc attcctttga catctacgac tttgacaagt
      301 ttgccgtttt tataaaatgt gaagtcggga atatactcga tcttgcgata cttctttccg
      361 tctagttcaa atttcggcat cagctcaaat ctttcctgaa gttttacttt ccagccattt
      421 gcttcagctt gccacaaggc tagatcgtaa tactctgctt ctgcgataga atcaaacttg
      481 atacctcgat gaacagtttt tttattacgg tatttattca tgcgatacta cctttcactg
      541 gttttatgcg cttgtctgct gtttgttgga atttcagcgc ataaccttct gaattcttaa
      601 atatcctaga aacaattctt tcaccgtagg cttctcttag ttcaggacca gataagtttg
      661 ttgtgatgat cgttgccttg ttctgtctgg cttctaagag cgtgtttaac gtgttgtttg
      721 taaactgcct actatttgat accccactac ctaattcagc tccaatatcg tcaaaaacca
      781 ccaaatcagt tgttttaata tcggctataa gcgatccttc aatttctttt ctcagttcag
      841 tattgttata agaaaacttt atttgctcta ataattcttg atagcttata aaaagtattt
      901 tcttgtcata atttgagcgc tcaagtattt cccaagctgt ggccattgat aagtggcttt
      961 ttccgcttcc tgatttccct gatagaatga aatgtgcagg atggttcagc agaacctcat
     1021 tcgtatagct ttttgctttt tctaaagcga tttttgtttc ttgatccact acgtgataat
     1081 tttccatttt gcatttaaac aaagttttat ctgttaatac cgaaccattt tgaaaaaaac
     1141 tcaacgctcg tgcttttaag ctatcgttat atatccgttc ggtctgtata tcctctttca
     1201 cacgtaacgc tttataacca caactcatgc atgttggttt acaacgttct gaaccatcct
     1261 tatttttagc tcgccaacta tacaaaggtt cgctacattc tggacatttt ccgctttgca
     1321 ctaatactct tcttattagt ttctccatag catttgctag gctttccatg tgatgcatct
     1381 cctttttaaa ttggcaagtc gtcatattca ctaggattgc tgtactgtag tttttgactt
     1441 tgctttttat gattcttctt gtctgctttg atttcgaatt tgagcttctc aaatttttct
     1501 ctcaatttct tagcacttct aatatttcca aaccaaaatt catttgtagg tagccaattg
     1561 atcacatact caatcgcttc tatagatgct ttatctcttt cttccatcaa cctgattgtg
     1621 tctgcccatt tttcgatatc tactttgttc atttcttttg gaaaatcttc agttaaatta
     1681 ctttgcaact ttttagcaag gcgtaagtgt tcgtcagaat acttaccttt cttttcttct
     1741 ttatctatat ctttatcttc ttctatatct ttatctgtac cgtcacgtga cgtcacgcta
     1801 acgtcatttt ccaatttgag acgttcctgt ctctttcttt ccctgtattt acggtttctt
     1861 tcagcatttt ttagccttac tttatccata ccctcgatat tttgatgttt ttcccaatta
     1921 ctgatggcaa ttagtccatc actgcttaga tcaatcatgt tgaaatttgc caatgtagtt
     1981 agcgctaagc gaaccgtatt tacgtttttg ccaaacaatg tagcaagcat ttcttcggta
     2041 taaggcatgt tcctctggat atatatcaga ccatcgtcgt tagtctttcc tgctaaaact
     2101 agtaatcgaa tccatataac gatgatggca tccgactcag gaacagcttg gattaatcgt
     2161 attttttcat cgtcaaacat agtagtttta agtttgatcc aacttatctc agccaaattt
     2221 atcctcctat ccttaacttt ttaattgttt cctggtttaa cttgatccct ttgatttgat
     2281 atttattttt gaaattgatc acacctattt tgtgtttctc tgtgtgatgg attctgcaga
     2341 gtgctgcaaa tgtgtactct gaatgatcaa cttctttgcg ctttcgtctt cccagcgctt
     2401 tgtcaaagtg atcgatatca gctcctgttt tgccacagat gcagcagact ctttttgtaa
     2461 tgcatttgta gaagtaatac tcttgattcg ctggtaaaat ctcatatcct tctttgaaag
     2521 gaatatgatg ttcaaagatg aaatctagga tgatattcgc taagatattg gcatcactca
     2581 cggttgtatt cgattcgtct ttgaggctta tttcgcgccc tgtgacacct tcaaaacgga
     2641 agtagaagaa ttccttccag aagtccgttg gcatgcctgt atcgatgaaa atatcgccta
     2701 tgagcgcata gatgaagttt ctttgctgta ctgtgaaccg tcgtggatca ataaagcgaa
     2761 tttcaatgac tcgatcgcca tcatatccgt catacatcgt tttcagccgt tcgatattta
     2821 cttcttcgtt gattgttgca ccaatgtcgt tacctttaaa tttctttagt accgccgagt
     2881 atgaatctat tagtggttta aacactcata tcacttctct tttgtttctt ctctgtactg
     2941 atcttcaagc caattaacgc ctcgttttag aatgcccaag tctctcttgg tccatttact
     3001 gtcatcagcg gttatggaag ctgcatcagt caatgcaaca attgcttcat caattgattt
     3061 ttcgtacttg ttagcaacca
//
LOCUS       DAJSVF010000174         3076 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSVF010000174 DAJSVF010000000
VERSION     DAJSVF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.183"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            173..787
                     /locus_tag="ON550_002890"
     CDS             173..787
                     /locus_tag="ON550_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3955909.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(811..1161)
                     /locus_tag="ON550_002891"
     CDS             complement(811..1161)
                     /locus_tag="ON550_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3955910.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1155..1418)
                     /locus_tag="ON550_002892"
     CDS             complement(1155..1418)
                     /locus_tag="ON550_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR3955911.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(1759..1938)
                     /locus_tag="ON550_002893"
     CDS             complement(1759..1938)
                     /locus_tag="ON550_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955912.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(2319..3026)
                     /locus_tag="ON550_002894"
     CDS             complement(2319..3026)
                     /locus_tag="ON550_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955913.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
BASE COUNT          941 a          596 c          472 g         1067 t
ORIGIN      
        1 aataagttat cagtagacac ttacaaaaaa ttctcatact atttcctcca tcattagttt
       61 ttgtctatat aaggtatacc ctaactagac acgaccgcga cattgaaaaa tctggttttc
      121 tcgtgtatga ttagggtgtt ttttaaatag ttgcagaaag taggtaaaaa ttttgagagt
      181 tgcatacata cgtgtctcat ccatcgatca aaatgaacaa cggcagattg aagaaatgaa
      241 aaaattcggt gcagagagaa ttttcattga gaaacaatct ggcgcaacga ttactcatcg
      301 acctgttttt caagaagcac tggatttcgt ccgagatcaa gatattttta ttgtggaagc
      361 tattgatcga cttggaagaa attacgacga gatcatcgct tcagtcaatt acttgaagaa
      421 gaaaaatgtg cagctgatca tcacaagtct accgatcatg gccgaagcaa ttggcaatcc
      481 tctcctggat aaattcatta aagatttgat cattcaaatt ttggccatga tcgctgaaca
      541 agaacgtacc gaatctaaac gacgtcaggc acaaggcatt aagattgcaa aagcgaacgg
      601 tgtttataaa ggacgtccta agctatacag tgccaatgca aaagatcccc aacgtcgtct
      661 tgtttataaa aacatcgttg aggatctaaa aaaaggtgtt gcgatcgcta agattgcaaa
      721 agactacaat gttacgagac aaaccgtata tagaataaaa aaagacagca tggtcaatca
      781 cgaatagttg atcatgctgc tgtttctatt ttatttgctt actttttgaa aaactgttct
      841 aactttcatc aataactcct caactaaact atcatgcgct ttttccaaaa aaatacactc
      901 tctggcttca taatcaattg tcgttagttg atctaataat acttttcctg ttgtcttcat
      961 ttcatattct gagtttatat ttacatatag cggatagttt ctagttgtac tagaaattgg
     1021 agcaactgta acaacgttac tataatccgc aataatatca tgacttaaaa caatataagg
     1081 tcttttacct gtctgttcgt gtccagatcg aggtgctgta ttcaataata gaatatcgcc
     1141 ttgtctaggt tttctcacca tttttcatta cctactgctt cacctaaatt ttgaatcgtc
     1201 acatgatttc tttcatcagt gtaattttca aataactgtt caattgttaa ttcattttct
     1261 atattagccg cttcgataat tagccgcttc tgaccgttta cttcttcttg tattatcttc
     1321 actttgtctt ctgtatgaat tccaagatat tccataatat actttggcaa tctaaggcca
     1381 ttactatttc cccatttttt tataggtaac atttccatag tagacacctc tttctgtata
     1441 tacatagtat aacatacata agaaaaaacg tatatactta attatccttt tctattaaat
     1501 tagattaaca ttccatgaat ccataaacta tgttaacatt tatatgaaaa tagtcgaaag
     1561 gaaatcataa tatgtttaag aaaacaaaat ctttagaatc aggtaagaaa aaaattgatc
     1621 taaaaataat ctcttctgga tactttaaag atttcatagt tattgataca aaatggaata
     1681 aaaaggacat agaaaatatt ctaaatagta ggaacaatta agtgctatca cttttgtttc
     1741 ttcaaatgaa aagtgacgtc acttttttag tgctggtata tcaaatcctg ctttttcttg
     1801 cgcgatcaat agatctaacg caatacacat aatcttagat tcactttttc cgtacttctg
     1861 ctttagttgc tgcaattctt tttgtctctt ttcatctaat gtcacgggta accgtttatt
     1921 tcctattgca attggcatta gtactctctc ctttcatcga aataacccta tgaaaaatat
     1981 ggctgcaatc atcttgaata gtagactcac aagggctaat acaaatcgcc aaaagatcaa
     2041 acaaaatacc agtattccta agaatactac gaagtttaaa tgtacagctg cagcatgacg
     2101 cataagtatc tcgccaacaa acagtacgaa caatagagga atgattttat tcatatggat
     2161 ctccttcttt ctcttaggtt atcgggcttt gcccgcttat ttcgcaccac ctaagcggcg
     2221 ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttaatttca aggttttttg
     2281 ttcttatttc acacatcctc acttttcggt ataaatggtt atttaccttt tttcctttca
     2341 aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc tgtatcgtcg
     2401 taagtgtagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc tgggttatct
     2461 acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata atccattccc
     2521 attaacacga tagattccgt cccacacgtc aaaaacctag taaatacaag gtaataatcg
     2581 tctaccgtcg catttttgct gtttagctct tccatttctt cttccattaa ttccatgctc
     2641 tcgaaaattg aataatattc tgattctgag taatagtgtt tcctacaaat cgtgtagctg
     2701 acaaagtttt cgaccagctc actcctttct aggtacacat tcatgatgta tttttctgtt
     2761 tctagtcgta gaagctctgt tccttttttt accgtttcag gacgacgttt atacacgtct
     2821 aaatgattat ggctgactgt gaccttcgtt tgacttgttt cgctttccat atacaactgt
     2881 tcttccactc tttgaataaa gtacttcatt tcttccatac aactaaagtc tttttgaatc
     2941 cgtctgatta cttcattcaa gtgttctttg cttgttaaaa attgcgaaac atcttctgtt
     3001 gttagctgat aaaccacctt attcattatc tttctcctcc gattcaatcg tataaaatgc
     3061 accatttttt tcgata
//
LOCUS       DAJSVF010000175         3071 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSVF010000175 DAJSVF010000000
VERSION     DAJSVF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.184"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            23..982
                     /locus_tag="ON550_002895"
     CDS             23..982
                     /locus_tag="ON550_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3955914.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1115..1321
                     /locus_tag="ON550_002896"
     CDS             1115..1321
                     /locus_tag="ON550_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3955915.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            1332..2351
                     /locus_tag="ON550_002897"
     CDS             1332..2351
                     /locus_tag="ON550_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3955916.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            2540..2613
                     /locus_tag="ON550_002898"
     tRNA            2540..2613
                     /locus_tag="ON550_002898"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2574..2576,aa:Ile,seq:cat)
BASE COUNT         1044 a          510 c          597 g          920 t
ORIGIN      
        1 gaattataag gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat
       61 aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg
      121 ttcaagacaa acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga
      181 atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc
      241 cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat
      301 tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa
      361 aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca
      421 tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta
      481 tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca
      541 taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc
      601 gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc
      661 gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca
      721 gatcagtaat gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag
      781 aggcctaaac gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga
      841 tttcaattca gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc
      901 tagaaaatca ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga
      961 tgatctgtct aacttaattt gacaattaag aaagataaaa ttgaacaact ggacgggcaa
     1021 attgatgcct tgctttcaga aagagaaata ttgatagaag aaaacaagcg attgaaaaag
     1081 aaagtagccc aaccaatgaa gggaggtgga tagtatgaac aatagaacct ttgatatttt
     1141 gaaatggatc gctatagtag ttatccccgc tttagcgact tttgtcggtc ttgtcggaaa
     1201 aggtgttaac tggcggtaca cagatataac ggtgatgatt attacaggtt ttggagcatt
     1261 tctagggagt attttaggtg tatcaaatcg aacgtataag aaaatttcat cagataaata
     1321 ggaggataga tatgaaaaag aaaattacta ttactgcgat gagcctgtta acggctcttt
     1381 ttttattgcc aattaacgga tttgcctata ctatcaacaa tgaatttaat ttgggcgcaa
     1441 atgaaggtag ctcacaagta gcaaataatc agtatatttt actgcatgaa acggctaatg
     1501 aaacagcaac aggacgcaat gaagcgcagt atatgcaacg ttcatggact agcgcttata
     1561 ctgcttatat tgtgggagac ggcggaattg tttatcaagt cggtcaacct ggttatgtac
     1621 agtacggtgc tggttcgtat gctaatgcca acagtcctgt gcagattgag ttacaacaca
     1681 cacatgataa agcaacgttt gagaaaaact acaaggcata cgttgaattg gctagagatt
     1741 cagcaatgaa atatggtatt ccattaacgt tggacactcc ttataaccaa ccgggaatca
     1801 aatcgcattt atgggtaaca caaaacatct ggggcgatca tacagatcct tacggttatc
     1861 tttctgaaat gggcgtaagt aaagaaaaat tagcatatga tttagctcat ggatttaccg
     1921 atgaaaatcc aacaacttct gaaaacaagc ctgtcattga tccaacacga gctggtgcag
     1981 ctaatcctac actgacagat ggaacgaatt acgcccacat tgatcagttt ggagaaatcg
     2041 aaaatgcaaa tttgcatgta gctggatggc acattgctaa ctataaatac gagtatatct
     2101 tcattatgga ttacaatact gggaaagaat tagctcgagt aagagctgat ggaatttata
     2161 gatcagatgt aaatcaagct tataatactt ctggaaatgt tggctatcat gtatctttta
     2221 acatgcgtaa cttccctaat aagaaagtct atgtcatgat gcgggcaacg aatgatccag
     2281 agggaaacac taaaggcggt gcgcaagatt tccatgacaa acgttggtat ttaaatattc
     2341 ctaaacgata aaaatagctc ctcgttgagg agcattacat aactatattg acaactataa
     2401 aaatcattcg ataaaatagt gatgttatcg catatcttca ctatcaccca taatagtcac
     2461 actccaagct atgcgataac aggtttgttg ccacacattc tactggttga ttgtttatgg
     2521 ctttatgtgg caacaaccag tacccttagc tcagttggtc agagcagacg gctcataacc
     2581 gtccggtcgt aggttcgagt cctacagggt acattaacgt agccatttga atcgttctgt
     2641 gttagaattt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     2701 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatatt
     2761 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     2821 tgagatgata ataaagaaga gtttaaagcg caccccaagc cacttcccca taagtgtgtt
     2881 acgctttaaa ctcttttata tttgaagcta ttaaaaggca taccatattt ttgaaaaaaa
     2941 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     3001 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     3061 aaatatctcc t
//
LOCUS       DAJSVF010000176         3020 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSVF010000176 DAJSVF010000000
VERSION     DAJSVF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.185"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(287..649)
                     /locus_tag="ON550_002899"
     CDS             complement(287..649)
                     /locus_tag="ON550_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955917.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ON550_002900"
     CDS             complement(723..1103)
                     /locus_tag="ON550_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR3955918.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="ON550_002901"
     CDS             1167..2360
                     /locus_tag="ON550_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3955919.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..>3020)
                     /locus_tag="ON550_002902"
     CDS             complement(2437..>3020)
                     /locus_tag="ON550_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3955920.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          941 a          578 c          498 g         1003 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca
//
LOCUS       DAJSVF010000177         3011 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSVF010000177 DAJSVF010000000
VERSION     DAJSVF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.186"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            177..359
                     /locus_tag="ON550_002903"
     CDS             177..359
                     /locus_tag="ON550_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR3955921.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="ON550_002904"
     CDS             462..746
                     /locus_tag="ON550_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955922.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="ON550_002905"
     CDS             762..998
                     /locus_tag="ON550_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955923.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="ON550_002906"
     CDS             1020..1274
                     /locus_tag="ON550_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955924.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="ON550_002907"
     CDS             1356..1541
                     /locus_tag="ON550_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR3955925.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="ON550_002908"
     CDS             1608..2003
                     /locus_tag="ON550_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3955926.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="ON550_002909"
     CDS             1993..2370
                     /locus_tag="ON550_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955927.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="ON550_002910"
     CDS             2392..2766
                     /locus_tag="ON550_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955928.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1059 a          507 c          523 g          922 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcatttaaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc t
//
LOCUS       DAJSVF010000178         2999 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSVF010000178 DAJSVF010000000
VERSION     DAJSVF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.187"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(254..589)
                     /locus_tag="ON550_002911"
     CDS             complement(254..589)
                     /locus_tag="ON550_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955929.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            complement(619..2211)
                     /locus_tag="ON550_002912"
     CDS             complement(619..2211)
                     /locus_tag="ON550_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955930.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2231..2653)
                     /locus_tag="ON550_002913"
     CDS             complement(2231..2653)
                     /locus_tag="ON550_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955931.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            complement(2692..2970)
                     /locus_tag="ON550_002914"
     CDS             complement(2692..2970)
                     /locus_tag="ON550_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955932.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
BASE COUNT          767 a          602 c          428 g         1202 t
ORIGIN      
        1 gcttttgccg ctttgtctgt gactacaaaa gctctgcctg tttgaatttc tgtcacaaaa
       61 acaagattgt ctctgctttt ttcaacaaca actccaatcg ttgaataagc catttcttct
      121 tcctcctttc ttttgatata atgtagacgg aaatcgcacc actcaatgaa ttggcggaaa
      181 acaattcagt gaaaaaaaag agtcctggac aatcacgttc aggactcttt tacttctttt
      241 attcacttaa caactaacat tctgtgagtg aaatagactc ttcaatgctt ttacacggga
      301 agttattgat cccctcttca ttcatttccc aagtagtata ataaggcgct aacttgccct
      361 catattcttg aatgataaaa tatttttcac ccttcacatg attacaacag tacacaccag
      421 tagctgggta ctcaacgact aaaaattgat ttggatcaaa ctgttcgttt atcaacattt
      481 ctcttatctg aaacaaatgc tctttttcta aacaatcggg aagttgttcg attcgttctt
      541 ctcttaatga tccttctata actttccatt gatctaaata ttgtttcatt gctattcctc
      601 ctactgtctt tactgtcttt actgtctttc ttgttccatt tggattcttc gttgttgctc
      661 ttcgtatgct ttacttgcgt ggtatttttc ttcatgggca ttaatcgttc gatttaattg
      721 acgcaaactc cgctgcgtga caactctttg ttggaattgt ttcctgcgct tcatgaattc
      781 ttcgtaacta tatttgtatt cattcgttgg caattgttta agcgttcgaa aggatcgttc
      841 attgtaaata gattgcgtct gatcaacact ttctattaat ggttttattt gactatcctg
      901 tctcttcagc tctgttagga tggcatttcc tgtgcgcttt tttaaatcaa agagagcatt
      961 tttctctgca tcctttgatt ctctcttgct ttccccataa agactttttg cttcttcttt
     1021 caatgctttt gaaatctgta tatattcttg gtattcaggt tcttttgtta acacatattg
     1081 ggttaactcg tcgagtttct ctcttgtgta tggtgaaagt ttagttcgat tttcatcccc
     1141 ataattccat tccgaacgtt tgtcaggtaa ggttttatat acttcattta ataagttcat
     1201 tgcgtgtcga tcataacgta ctggctcttt taccactttt gttaagctgt tacgcaaatc
     1261 acttttccgc gttaataaat ttcgtttctc catgcctatc attcgatcct tttcataaga
     1321 aaaacgttct aattcatgca cataattggt cttcattgcg tctaatgttg cttgtcttct
     1381 ctttcctttc ggtacgatat aactcacacc attttcgacc tcagtgtatg gtttacgcgt
     1441 attttctttt tcaacaactg caaaatgaat atgaatatgc ttagtgttac ggtgaatggt
     1501 agccatccaa tatcccgtat cattcagttt ttctttctca aacaacgtct ccatcatttt
     1561 ccttccagca gcttttagtc caacttcatc taaagtatct gttttgggat cgtacaatcc
     1621 tattttttca agatacgaat tatcatgaga aacaacatct tgataaatga ttgagccttt
     1681 ttgttgccct ctttggaaga tctgcttaat ctccttcaga tcacttcctt ttacttcgtc
     1741 tgaagatata gaaaaaactc caggaagagt tcctgaagtt tttttgtgtg gttctgcttg
     1801 atatttttcc attgcttgat tcaaacgttg atattctatt ttttcccttt ccgttagttc
     1861 ctttttttgt tctaacgcat agtgacgcgt catataagaa agatatttgg taaaatcttg
     1921 ttcctctaac ttgtcaaaat tgattccata ttccatcaac gattgcgctt cattcgacgt
     1981 ttcactgaat tctttatccg tcttactctc cgaaaaagtt tctccttcag gaatataaaa
     2041 atcctctaaa gcattcttga ttcgttgcag ttcctgttct tcttcgagcg tgcgccgttc
     2101 catttcttcc agcgcttttt ctcttgtcat ataatcaatg tatgttgaaa aagatttttt
     2161 attgggcaag gtaaattgac aagtgagaat gactgcaggt gatttactca tcacgttctc
     2221 cttctaattt ttatcgtttt aaaaaccttg ttggatcaaa tacttcactt tttgtagtca
     2281 ctgattcgct tattgttttc tttatcttag gagaatattt cttcgtttga tttgctttta
     2341 tcttgccttc cacacgctct ttcgcttctt gataaacagt tgaatttgta ccaattaata
     2401 ttcctgtatc ttttaaatga agatctgcaa actccgaaac catttcagtt aaaatagaaa
     2461 cttcttttcc aattgcattt agtttgattt gactgggact tatcttttct tcagttttgg
     2521 gatcatacat catacacaaa tgtctaactg cttcactata attggatata tcgtcatgag
     2581 tagaaattat ctcatcaagt acttcgatat gcatatcata aagataaata tgttttcttg
     2641 ttgctttcgc caaagttgat ttcctccttt tataaattca caatattatt tttactgttc
     2701 aattaacaag gcgattaatt gattatttgt tgcaatatat tcttctaaaa ttttcgtatt
     2761 ataagtttga atatccacaa atttttctaa caaccgatca aattgatctt ttttataatc
     2821 cagaagagcg tgatcaaaat cattttcgat caaaactttt aggtatcgat ttcggctcca
     2881 tttagtccca gtacgttcac ttaattcaat acatcttcgg tcaattcttt gaatcgtttt
     2941 ttcatcaata tctcttattt ttacttccat aaatatctcc ttttgactat atgttataa
//
LOCUS       DAJSVF010000179         2947 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSVF010000179 DAJSVF010000000
VERSION     DAJSVF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.189"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(449..865)
                     /locus_tag="ON550_002915"
     CDS             complement(449..865)
                     /locus_tag="ON550_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955933.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="ON550_002916"
     CDS             complement(867..1148)
                     /locus_tag="ON550_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955934.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="ON550_002917"
     CDS             1948..2922
                     /locus_tag="ON550_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3955935.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg attgctggta gtagtcgtgt
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAJSVF010000180         2894 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSVF010000180 DAJSVF010000000
VERSION     DAJSVF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.190"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            256..1224
                     /locus_tag="ON550_002918"
     CDS             256..1224
                     /locus_tag="ON550_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3955936.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1217..2248
                     /gene="vanA"
                     /locus_tag="ON550_002919"
     CDS             1217..2248
                     /gene="vanA"
                     /locus_tag="ON550_002919"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3955937.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2254..2862
                     /gene="vanX"
                     /locus_tag="ON550_002920"
     CDS             2254..2862
                     /gene="vanX"
                     /locus_tag="ON550_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HCR3955938.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYAASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          856 a          556 c          706 g          776 t
ORIGIN      
        1 ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat ataatttttt
       61 aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa
      121 acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag
      181 agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa
      241 gggttggtct taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag
      301 gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc
      361 aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat
      421 aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt
      481 tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc
      541 actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt
      601 cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg
      661 ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc
      721 cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat
      781 agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc
      841 gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa
      901 caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta
      961 gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat
     1021 gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat
     1081 caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat
     1141 tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt
     1201 gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga
     1261 ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa
     1321 atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc
     1381 ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat
     1441 gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt
     1501 ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc
     1561 cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt
     1621 gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa
     1681 agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc
     1741 aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga
     1801 atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt
     1861 cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg accaaatcag
     1921 gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa
     1981 cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac
     2041 ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt
     2101 acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata
     2161 cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg
     2221 cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat
     2281 gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga
     2341 aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg
     2401 cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt
     2461 taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac
     2521 ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac
     2581 gcggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta
     2641 gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat
     2701 catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc
     2761 atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac
     2821 gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg
     2881 gacaaactat ataa
//
LOCUS       DAJSVF010000181         2857 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSVF010000181 DAJSVF010000000
VERSION     DAJSVF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.191"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            562..843
                     /locus_tag="ON550_002921"
     CDS             562..843
                     /locus_tag="ON550_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955939.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="ON550_002922"
     CDS             845..1261
                     /locus_tag="ON550_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955940.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="ON550_002923"
     CDS             complement(1422..1688)
                     /locus_tag="ON550_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955941.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="ON550_002924"
     CDS             complement(1646..2620)
                     /locus_tag="ON550_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3955942.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DAJSVF010000182         2841 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSVF010000182 DAJSVF010000000
VERSION     DAJSVF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.192"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..239
                     /locus_tag="ON550_002925"
     CDS             <1..239
                     /locus_tag="ON550_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3955943.1"
                     /translation="GAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLY
                     ICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            469..699
                     /locus_tag="ON550_002926"
     CDS             469..699
                     /locus_tag="ON550_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HCR3955944.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(922..1020)
                     /locus_tag="ON550_002927"
                     /pseudo
     CDS             complement(922..1020)
                     /locus_tag="ON550_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1285..1740)
                     /gene="tnpA"
                     /locus_tag="ON550_002928"
     CDS             complement(1285..1740)
                     /gene="tnpA"
                     /locus_tag="ON550_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR3955945.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1983..2841
                     /locus_tag="ON550_002929"
                     /pseudo
     CDS             1983..2841
                     /locus_tag="ON550_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          917 a          512 c          501 g          911 t
ORIGIN      
        1 atggcgcaaa aattattgct ataactaact atattcattc acctatagga aagtctgcag
       61 acttagtttt acaaacagcc attgaagaat ttcttaatgg tggatcttta gctggaaaaa
      121 tatcacaact ttatatttgt gatctattag ttcatggtta tgaacaaaac aataatattg
      181 attcagttga attaagagaa aaagttttgc gctctattat tgataaaagt attgagtgaa
      241 ctacccccac ttagctaaac ctaacggttt ttaacgattg aagtaggcaa ttcttgggat
      301 gagagcatac ttgatagctt attttttact aatagcggtg gctcttattg accaaatatc
      361 cctgtagttc ctacggttct tgattttatc taaagatatt tttatcgtgg aagctattga
      421 tcaacttgaa agaaaaacgt gtagctcatt atcactagtt tatcgagtat ggatgaagca
      481 atggatcatc ctctcttaaa taaatttatt aaggatctga tccttcaaat tttttccatg
      541 atcgatgagc aggaacctac tgaatctaaa cgacatcggg cacaaggaat tattaagatt
      601 gctaagcgaa tgttttctat aaagggtgtt ctaagctata cagtgttaat tcaaaagatt
      661 cccaatcatc ttgtttataa gaacaacgtt gaagattaaa aaagtgtttc aatgctaaaa
      721 ttgcaaaata cttcaatgtt atgagacaaa ctgtctataa gaatcaattt ccacccattt
      781 catcacttgt taaaagttta tgatcctttt gatttttata ttaatttatc tgtatttaat
      841 acagatttta tttattagtt catctgccta ttgtatttta ttttgttgcc ttaccatctg
      901 aagtaaatat actcttaatt gtcaaattaa gttagacaga tcatcaatac ttacgtaact
      961 caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaagat tatttctgcg
     1021 ccagcatttt ctaatgtttg aatttgtgtt ttgtaatctt ggttgcttgt gctaattctt
     1081 gaatagccgt aaattgccaa aatttcatca ccctttctgg acataagttt ctgacaccag
     1141 ttaaaatcaa gtctatcagt aacccataca cccgtgagga cgggtgtatt tttaatcccc
     1201 ctagaagggg tcgacactcg ctgcccctta aaaggggatt tttaaaagac cgccaactgc
     1261 tcatcttcac tcacttactt ctctttattt tttaaatggt tctacgtact ctcttttact
     1321 tataatatat cgaatgcgat catcagcttc ttgttcacgg atgtattttt catttgtctt
     1381 ttgattcaat ccgactgtac tcacatagta tctcttcgac caaaaggatt tgtttccgta
     1441 tttatatttg agatttgcgt gctgctcgtg gattagtacc gcactacgac ctttcagata
     1501 ccccataaaa ctggatatac atatttttgg tggtactcga acaagcatgt gaatatgatc
     1561 tagcattgca tgtgcttcta tgatttccac atctttcatt tcgcacaact tcctaagtat
     1621 ttttccaaga tcttttcgta attgcccata gattactttt cgttgatatt ttggtgtaaa
     1681 aataatatga tacttacaat tccaacgagt atatgctaaa cttttatcgt catttgacat
     1741 gaaaaaactc ctttcgatct gatgagcggt tggtagccac tcactagtat atcgaaagga
     1801 gtttttatcg ctaaagatac ttcttccacg cgtagaaacg cgcggttttc ttgtttcgca
     1861 cgctttacgc acaaaactga ctgaagtcat taagaaaata aaagggtcta aaacttttaa
     1921 gttttgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg tcggtatttt
     1981 ttatggctaa gtatactttt gaacttaaat taaagatcgt tcatgattac ttggatggta
     2041 aaggcggaag tgattatctg gcaaagaaat attccattaa ggctccatca caagtgaagc
     2101 gctggatcaa tgcctatcaa gaatttggtg aagagggtct agtaagaaag cgacaaaaga
     2161 aaaaatattc tgttcaattt aagctggatg cgatagagtt atatctaaca agtgagctgt
     2221 cctatcgtga agttgccaac acacttaaca tgaacaaccc taatttgatc gctagttgga
     2281 tgcgtcaatt ccgtgagggt gggatcgatg gactctcaaa aacgaaagga tgtccaccta
     2341 tcttgtctaa gaaaaatgaa cccaaaaata agaaaaaatc tacaactaaa gcaacgtcta
     2401 aagaacgtga acgcatcgag gagttggaga aacgagtccg ttcccttcaa attgaaaatg
     2461 cctttttaaa agaattgagg aagctgcgaa aacaggaagc ccaacaacga cgaatgaagc
     2521 aatcgcacga atcatcgcaa gcctccgagg accgtttaaa ttagttgagc ttctagaaac
     2581 tttaaagttc cctaaggcaa tctttatgta ctggcaaaaa cgattggacc gtaaaaatcc
     2641 agatcaagag attgaagcgg aaatgcttaa agttcgggag aagcacaagg attatggctg
     2701 tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa agaaagttca
     2761 gcgcctgatc aagaagctag gaataaaagt aacgacatac acacgtaaat cacggcgcta
     2821 caattcttat cgaggacaag t
//
LOCUS       DAJSVF010000183         2783 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSVF010000183 DAJSVF010000000
VERSION     DAJSVF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.193"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            88..984
                     /locus_tag="ON550_002930"
     CDS             88..984
                     /locus_tag="ON550_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3955946.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1083..1322
                     /locus_tag="ON550_002931"
     CDS             1083..1322
                     /locus_tag="ON550_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR3955947.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            1371..1454
                     /locus_tag="ON550_002932"
     CDS             1371..1454
                     /locus_tag="ON550_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HCR3955948.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            1577..2314
                     /gene="erm(B)"
                     /locus_tag="ON550_002933"
     CDS             1577..2314
                     /gene="erm(B)"
                     /locus_tag="ON550_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3955949.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            2484..2744
                     /locus_tag="ON550_002934"
                     /pseudo
     CDS             2484..2744
                     /locus_tag="ON550_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT         1081 a          418 c          477 g          807 t
ORIGIN      
        1 aaagagggct tttatatatt aatcacaaat cacttatcac aaatcacaag tgattaatca
       61 caaatcactt gtgatttgtg attcttaatg atacaatatt actatacaaa aaaagaatgg
      121 ggcgtagtta tggagaagga aaaactaaaa atacttgaag aattaagacg tattttaaac
      181 aataaaaatg aagcaattat tatcttgaat aattacttta aaggtggtgt tggaaagtcc
      241 aaattatcga ctatgtttgc ttacttgaca gacaaattga atttaaaagt tttaatgatc
      301 gataaggact tacaggcaac attgacaaaa gacttagcaa aaacatttaa ggtagaattg
      361 ccacgtgtca atttttatga aggactgaaa aatggaaact tggcttcttc tattattcat
      421 ttgactgata atttagactt gattcctggc acgtttgatt tgatgttact gccaaaatta
      481 actcgctcat ggacgtttga aaacgaaagt agattgcttg ctactctttt agcaccttta
      541 aaaagtgact atgatctcat tattattgat actgtaccaa cgccaagcgt ttatacaaat
      601 aatgcaatcg tggcaagtga ttacgttatg atccctttac aagcagaaga agaaagtaca
      661 aacaacattc aaaactatat ttcctatttg attgatttac aagaacaatt taaccctgga
      721 ctagatatga tcggttttgt tccttattta gttgatacgg acagcgcaac gataaaatca
      781 aacctggaag aactgtacaa gcaacataaa gaagataact tggttttcca aaatattatc
      841 aagcgaagta ataaagtaag tacctggtct aaaaacggaa ttacagaaca caaaggctat
      901 gacaaaaaag ttttatccat gtatgagaac gtattttttg aaatgcttga gcgaattatt
      961 caattagaaa acgaaaaaga atagaatcac aaatcacaag tgattaatca caaatcacaa
     1021 atcacttgtg atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt
     1081 gagtgattgt gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg
     1141 caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg caccatatca
     1201 taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa ataagactta
     1261 gaagcaaact taagagtgtg ttgatagtgc attatcttaa aattttgtat aataggaatt
     1321 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
     1381 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
     1441 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
     1501 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
     1561 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
     1621 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
     1681 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
     1741 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac
     1801 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
     1861 taaaattgtt gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt
     1921 tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
     1981 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
     2041 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
     2101 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
     2161 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
     2221 gaaacacgcc aaagtaaaca atttaagtac cattacttat gagcaagtat tgtctatttt
     2281 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg
     2341 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     2401 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     2461 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     2521 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     2581 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     2641 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     2701 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacataa aagcccttta
     2761 aatgatgtgc accccaaaag tta
//
LOCUS       DAJSVF010000184         2737 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSVF010000184 DAJSVF010000000
VERSION     DAJSVF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.194"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            734..2611
                     /gene="ltrA"
                     /locus_tag="ON550_002935"
     CDS             734..2611
                     /gene="ltrA"
                     /locus_tag="ON550_002935"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3955950.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
BASE COUNT         1047 a          426 c          603 g          661 t
ORIGIN      
        1 tgcgacctgt tctgcagtga aaagctgtgg gttgcaagta gtctaaaaac tattgtaata
       61 actcttataa cgagaagtca aatgagtggg taacgccaca aagggcttcc actgatactc
      121 cgacatgcaa caagtagttt atgcagacca acgttgtgtg aagctcggtg aagtcggctg
      181 aaggataccg taaggtaaaa gaaatagtga ataccacgaa agttctttag aggtaaaggg
      241 tgtatccgat aagtcgggtg tccaaaaaaa cttaatatgg tgagtatgta caaacgtact
      301 gacgaactag cgaatgtact gtccgaaagg catagctcct agaaatggga gagacagtga
      361 gcaaaaaatc actcattgtg ctaacttgtg gttgagtaaa agtcgttgtt atgaaagacc
      421 atgctgcgtt aaaggcgtag gagaagttag ggggcaaaag ctagcatcta agttaaggca
      481 tgtggataga tataagggaa caggggaagc catcaacatg gagagtgcat actcgatgaa
      541 gtggtagtaa gaaatatgtg aatgacaaat aacttacttt agtgatggtg aaagtagagt
      601 cacgactgat gaaggttctg taatggaact ggaggaacag gctcgattcg tctgtaaaag
      661 tcatgaaacc cgttcaacca tgggattctc gtatgactaa aaaggtcacg agccaattgg
      721 agcgtgataa accatgacaa aagaaacaag tccaaaagat aggaagttgc ggcatgcaga
      781 atattatggc atgacggaaa tcttcgataa gatatactcc aaaagtttga agggagataa
      841 tttcaagaat ctaatgcaga ttatcatttc tgataacaac attgtcctag catatcgtaa
      901 tatcaagaga aatcacggta gtgtgacaag aggagaagat ggagtaacga ttaaagatat
      961 tgaaaatctc aaccgtaatg aattcgtaga aattgttagg aagcgattcg cacattataa
     1021 ccctcggaaa gtaaggcgtg tagaaattcc taaatataat ggtaaattaa gaccgttggg
     1081 tattccatca atatgggata gaatcgcgca gcaatgcatc ttacaagtat tagaaccaat
     1141 atgtgaagca aaattcaata atcacagcta tggtttccgc ccaaatagat cagcagaaca
     1201 cgcaatagca gattgtatgg cccgaatgaa tcaaagtcac atggaatatg tggtagatgt
     1261 agatatcaaa ggtttcttcg acgaagtcaa tcatactaaa ctcatgagac aaatctggtc
     1321 tatgggtata cgagacaaac agcttctctt aatcattagg aaaatgttaa gagcacccgt
     1381 agtacttcca aatgggaagt tgatataccc aacaaaagga acaccacaag gcggaatact
     1441 tagcccatta cttgcgaaca tcaatctcaa cgaatttgac tggtggatag caaatcagtg
     1501 ggaggaaaaa gatgccaaag agctaaaaca tcattttagg aaaaatggag cttgggataa
     1561 aggtaatatc tatcacaagc tcagaaagag tacaactcta aaagaaatat acctagtgag
     1621 atacgcagat gattttaaaa ttttctgtag aaattataat gaagcgaaaa aagttttcta
     1681 tgcaactaaa atgtggttac aggagcgtct aaagttacca atatctgaag aaaagtcaaa
     1741 aatcacaaat ctttcaaaag aagaaagtga atttttaggc tttacgatga aattagtgac
     1801 caaaggtaac aaaaaggttg ttaatacgca tataggtaca aaagcacaaa gcatgattaa
     1861 agtgaaacta aaaaaacaaa tcaaacgaat tcaaaaaagt cctaatagca aagatacctt
     1921 tatctatatt ggagtctata acagtatggt tattgggatt cataattact tcaaaattgc
     1981 gactcattgt agtgtagcaa tgcgaaaaat tgcaagagaa ctcagaccaa gttttgaaat
     2041 acgactacga gaacaaggaa tctcaaaaga aggacaatac ggaaagtacg aaggatacaa
     2101 gccttaccta cggtcaggac aaatacgata tataagaggg tttccaatat taccagtagg
     2161 gtatattcaa cacagacatc cgatgtgtaa aaaaagaaat attaataaat acaccgaaga
     2221 aggtagggaa gccattcata agaagcaaca aaccgtagaa gaatggaaga tatcgtggtt
     2281 gtgtagcaat ccagtgctta gtaagagagc aacgattgaa tttgcggaca atcgaatttc
     2341 aaaattcatc gctcaaaagg gaagatgtgc agtaacaggt gaggagctta tcttatctga
     2401 aatgcattgt catcatatta tcccttatca tgaaagcaaa agtgatagct atgaaaacct
     2461 agtgattgtt actgaggagg ttcatcgggt aatacacgct acacaaagtg aaacgattga
     2521 ggaacttcta aaatatctaa aattaaatcc aaaacaaaaa gaaaaactca atgagttgag
     2581 attaaaagtt ggtaacgaag aaatatctta ataaatattt gtaggacggg aaaacagaat
     2641 gtcttttata gacgagggaa cgccgtatgc ggtgaaagtc gcacgtacgg tgtggactgg
     2701 gggaaaagac ggagataata tcaaactctt acctatc
//
LOCUS       DAJSVF010000185         2730 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSVF010000185 DAJSVF010000000
VERSION     DAJSVF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.195"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..333
                     /locus_tag="ON550_002936"
     CDS             <1..333
                     /locus_tag="ON550_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR3955951.1"
                     /translation="KLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKK
                     TGGLRYAPALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKRED
                     FNEAQKIR"
     gene            349..1110
                     /locus_tag="ON550_002937"
     CDS             349..1110
                     /locus_tag="ON550_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3955952.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            1241..2575
                     /locus_tag="ON550_002938"
     CDS             1241..2575
                     /locus_tag="ON550_002938"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR3955953.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT          926 a          463 c          584 g          757 t
ORIGIN      
        1 aagctatttg aagagtcaga cgaagagacg attgcaggaa tcatcaaaca tgaactttgc
       61 cattatcatc ttcatctagc tggaagaggg tatcagcata aagatatgga tttcaagtat
      121 cttttgaaaa aaacaggagg gttgcgctat gcaccagcct tgacgaagaa acagataaag
      181 atggagtatt atcgatgcca agattgttct gccacgatct gccgcaagag aagaatcgat
      241 gtacgcaaat atcgttgtgg aagatgtcat ggagaaatca aatggattga gacaaaaaga
      301 gaagacttta atgaagcaca aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt
      361 gagcagagga atctaaaaga ctggaatcag ctattaaatt ttttagccgc acctgaagag
      421 ggaaattttg aggaactgac ggcacctaca cttattttag ctggaaactg tcttcctatc
      481 ttagctgata aagcagcaca aatgtattta aatggccaag tagatcaatt gtttttggtc
      541 ggaggggtgg ggcatgcgac tagaatcctt tatgagaatt ttgaaaagca aggatttcat
      601 tttgaagatg gcatgagtga aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat
      661 ttgcccgata aggcttttct gatagaatcg aagtctacaa attctggaga gaacgccact
      721 ttttcactgg agattcttca ttccttagga gctgtaccag aaaaaatctt attgatgaat
      781 gatccgacac tgcaacgcag gactcgagca acttttgaga aagtatggca aaatgaacaa
      841 actattttcg cgaatgctgt gccatttgtg ccggaaatcc ttcacttttc tgaagagatc
      901 atctttacag caaaagaact gaatcatcaa tggcctaagg aatatttcca tgcgctagta
      961 ttaggagaaa tggaacgtct ccatgacgat gagaacggct atggtccgaa aggaaaagat
     1021 tttattcctc acattgatat atcagaggaa gtttggtctt cttacaccag aattaaagag
     1081 tcaataaaaa cggatttttc tcgtacttga ccattttagc aagcgaatcc tcctttccaa
     1141 ataaatttgg ttgaaatttt caatcgttta taagataatg aggagcgtgt tgaatgtaat
     1201 agctaaaaga atggacgcta gataagagaa gaggattcaa atggaaaatc aaaataaatt
     1261 gtatcatttt gttggtatca aaggttcagg aatgagctcc ttggctcttg ttttacatga
     1321 acaaggtcta aatgtccaag gatcagacat tgaaaaatat ttctttacac aaagagattt
     1381 agaaaaagca aatatcacga ttctgccatt taacgcagat aatgtaaaac cagggatgac
     1441 gatcattgca ggaaatgcat ttcctgattc acatgaagaa atccagcgag caaaagaatt
     1501 aggtttggaa gtgattcgct atcacgactt tattggtcat tttatccaaa attatacgag
     1561 tatcgctgta acaggctctc acggaaaaac aagtacgact ggactgctct ctcatgtatt
     1621 gagtggtgtc cgtccaacaa gttatctaat tggagatgga acaggacatg gtgatccgca
     1681 agcggaattc ttttcatttg aggcctgtga atatcgccgt catttccttg cctattcacc
     1741 agactatgca atcatgacca atatcgattt tgatcatccg gattattata caagtatcga
     1801 cgatgtattt acagctttcc aaacaatggc tggacaagtg aaaaaagcta tttttgcgta
     1861 tggtgacgat gcttatttac gcaaattaaa agcaaatgtg ccgatttatt attatggtgt
     1921 cacagaaaac gatgatatcc aagcacgaaa catcgaacgc acaacttctg gttctgcttt
     1981 tgatgtctat catggggatg aatttgttgg gcacttcact gtaccagcat tcggtaaaca
     2041 caatatctta aatgctttag gtgttattgc agtagcttat ttcgaaaaac tggatctgaa
     2101 agaagttgca gaagaaatgc tgactttccc aggagtaaaa cgtcgattca gtgaaaagat
     2161 cgtagcagac atgaccgtag tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac
     2221 gattgatggc gcacgccaaa aatatccaga taaagaaata attgcagttt tccaacccca
     2281 tacgtttacg cgtacgattg cattgatgga tgaatttgca gaagcacttg atttagctga
     2341 taaagtttac ttatgcgata tttttggttc ggctcgggaa gaacaaggaa acgttaagat
     2401 tgaagacctt ggagcgaaaa tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc
     2461 tccattgtta gattatcatg atgcagtagt aatcttcatg ggtgctggag atgttcaaaa
     2521 atttgaacaa gcgtatgaaa aacttttaag cagcacgacg aaaaatgtac tgtaatacaa
     2581 ttactgcagt gtccttataa aaatagaaag agggggagtg gcaaaaatct tgccaagccc
     2641 ccttttttgc aacttatgat aaacggtgtt caaaagcttg catctgcggt aataagccca
     2701 atcaaatcaa aaacatgaga agctgttttt
//
LOCUS       DAJSVF010000186         2560 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSVF010000186 DAJSVF010000000
VERSION     DAJSVF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.196"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..379
                     /locus_tag="ON550_002939"
     CDS             <1..379
                     /locus_tag="ON550_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3955954.1"
                     /translation="YLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQR
                     VAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADS
                     ADIILELSGKKLKKVNKMNLEVE"
     gene            379..1755
                     /locus_tag="ON550_002940"
     CDS             379..1755
                     /locus_tag="ON550_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3955955.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2041..>2560)
                     /locus_tag="ON550_002941"
     CDS             complement(2041..>2560)
                     /locus_tag="ON550_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3955956.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          951 a          355 c          460 g          794 t
ORIGIN      
        1 ttatttatca ccaattgaaa atattagatt agtaaataaa tcagcagatg aaagtatctt
       61 gtttgaacta ggtttagata aaaaacaaat aaaaagaaat gttatgaaat tatctggtgg
      121 acagcaacaa agagtagcta ttgctagagc attggtatca gatgctccaa taatactagc
      181 tgatgagcct actggtaatc tagacagtgt tactgctgga gaaataatta atatattaaa
      241 gacattagct aaagatagaa ataaatgtgt aatagttgta acacatagta aggaagtagc
      301 agattctgcg gatatcattt tagaactaag tggtaaaaag ttgaaaaaag taaataaaat
      361 gaatttggag gttgaataat gataaaaaat gcatttgctt atgtaactag aaaaagttta
      421 aaatcattaa ttatcatatt agttatttta tctatgtcaa ctttaagtct cattagttta
      481 tctattaaag atgctacgga cagagcttca aaagaaacat ttgctaatat aacaaatagt
      541 ttttctatgg agataaatag acaagtaaat ccaggaacac ctagaggtgg gggaaatgta
      601 aaaggtgaag atattaaaaa gatatctcaa acagatagta tagactccta tgtaaaaaga
      661 ataaacagtg ttgcagattt agttgattat gatattattg aaactcaaga gactcttgcg
      721 aatcaatcac ctgaaagagc aaagaatttt aaaagaacag ttatgttaac tggggttaac
      781 gactcaacaa aagaaacaaa atttgtatca gaagcatata aattagtaga aggaaagcat
      841 ttagaaaatg aagataaaaa taaaatctta atgcataaag atttagctga aaaaaataac
      901 cttaaagttg gagataagat aaaaataaaa tctaatttat ttgatgctga taatgagaaa
      961 ggtgcagatg aaacagtaga agtagaaatt aaaggtctat tcgatgggca taatagcggt
     1021 gtagtaagtg cagcacaaga actatacgaa aatacattaa tcactgatat tcatacagct
     1081 gctaaagttt atggtaatac cgaagataca gctgtatacc aagatgcaac attctttgta
     1141 aaaggtgata agaatcttga tagtgtaata aaagatcttg ggaaattaga tataaattgg
     1201 agagaatata atttgattaa aagttcatca aattaccctg cattacaaca atctatatca
     1261 ggtatttact caatttcaaa taaattattt tttggctcat taatatttgc aggagttgta
     1321 gtttcattat tattattctt atggatgaat gctagaaaga aagaaatagc agtattacta
     1381 tcattaggta tatcaaaatt agaaattttt ggtcaattcc taattgagat ggtatttata
     1441 tcaatccccg cattcgttgg ttcctatttc ttagctcaat atacagctga caagcttggg
     1501 aataatatat tgaataaggt tactggtgat atagctaaac aaatagctaa acaatctgca
     1561 tctagccaat taggtggtgg ggcagaagct gaaggattta acaagacatt atcaagttta
     1621 gatatcaatg tattacctaa attcatgatt tatgtagtta tatttatgag tttagtactt
     1681 cttgtatcat tgattatttc ttcattcaat atattaagaa ggaatccaaa agaattatta
     1741 attgacaata attaaaattt tcggtgcttt ttagcaccaa aaatgagagt gtaatataaa
     1801 ccgtgtaagt aaccatcaat cctaggcggg tggttgcttc acggttttta tttttctaaa
     1861 ataagatcaa gaggtgatta cacgatggct agaaagaaaa gaaatcctga tgctgagaag
     1921 ttagctgaat ccattttgaa tacttatcaa cccgaatctg tagaggatat gcaagatgtt
     1981 ttaaaagatg tgtttggtcc cttatttgaa aaaatgcttc aaggagaatt gaataaccat
     2041 ttagggtatg acgcccattc taaagagcca aagaaacatg acaatcgacg aaacggttat
     2101 ggcaataaga cgcttaaaac gagctttggt aaagtaaaca ttgatgttcc cagagaccgg
     2161 gaggcttctt ttgaacctga gttgattcct aatagaaaac gagatgtctc cgacatcgca
     2221 gactgtagac aaaagctgtt ccaaactcgt aactcgagct tggaacagct ttttatctac
     2281 ttcgtttcga atttttgaac aaaaaaataa aggctccttg cctttccata gccaggtggc
     2341 taatttcttt aaattcattg cagcaaacac tagcatcgta tgcgtcgcga ctttttccaa
     2401 gcctcgatac ttggtccaac gcatcccatg cttttctttt gcatccgcaa atattcgttc
     2461 aatcgtttgt ttccgttttt tgtaaattga tttattcaga tctgttaggc gaagatgttc
     2521 cacttcatcc atcagatctc cccaaacatg tctcgtgatg
//
LOCUS       DAJSVF010000187         2544 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSVF010000187 DAJSVF010000000
VERSION     DAJSVF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.197"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..2109
                     /gene="acm"
                     /locus_tag="ON550_002942"
                     /pseudo
     CDS             1..2109
                     /gene="acm"
                     /locus_tag="ON550_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
     gene            complement(2134..>2544)
                     /locus_tag="ON550_002943"
     CDS             complement(2134..>2544)
                     /locus_tag="ON550_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3955957.1"
                     /translation="IAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNY
                     PKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLER
                     FLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          943 a          415 c          494 g          692 t
ORIGIN      
        1 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
       61 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
      121 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
      181 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
      241 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
      301 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
      361 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
      421 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt ttattataaa
      481 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
      541 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
      601 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
      661 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
      721 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
      781 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
      841 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
      901 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
      961 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
     1021 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
     1081 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
     1141 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
     1201 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
     1261 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
     1321 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
     1381 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
     1441 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
     1501 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
     1561 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
     1621 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
     1681 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
     1741 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
     1801 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
     1861 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
     1921 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
     1981 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
     2041 tttgctgtaa tcattggttt attgatagta actatctgtg cgggagcgtc aataattttg
     2101 tgtaaataaa ttgtcctcct gcaaaataat tagttactca gtaaacattg aaactaatgt
     2161 atcggttacc tgttgaaaac ctttatggct tctgtttaga aatttttgat tgtatgtatc
     2221 aaaaatgctg actagaaagc gttctagtga ttcttcattt tgaaactgct cttttctacg
     2281 gctgtatctt ttaatttgct tattgaaaga ctcgattaga ttggttgagt aaatggttct
     2341 acgaatgcta ggtggaaaat cataaaaagt taataagtct tggttttcta tgagtgactg
     2401 cgtcacttta ggatagtttt tcttccattt ctcaatcatg ccggataaga aggtattcgc
     2461 ttcttctttt gagttagctt gataaacagc cttaaagtca tcacagattt cttttcggtc
     2521 tttgacacgt actttatgag cgat
//
LOCUS       DAJSVF010000188         2538 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSVF010000188 DAJSVF010000000
VERSION     DAJSVF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.198"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..65)
                     /locus_tag="ON550_002944"
     CDS             complement(<1..65)
                     /locus_tag="ON550_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCR3955958.1"
                     /translation="MANNRRLEETFNDGWLKILTQT"
     gene            complement(55..366)
                     /locus_tag="ON550_002945"
     CDS             complement(55..366)
                     /locus_tag="ON550_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955959.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPDSLEYGLLIERCRYDWNRALGEFEQNFASELLGFIQHYALQEYIAGDGNG
                     E"
     gene            complement(363..1238)
                     /locus_tag="ON550_002946"
     CDS             complement(363..1238)
                     /locus_tag="ON550_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955960.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGARWIINLPKANYWKKVYLDSNRLYRYLRGQKTNVQIWTAEMWAQLWNMMYFNIGP
                     KVHEELNFCFATDPIEKFKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYAKAIKAVVR"
     gene            complement(1248..2408)
                     /locus_tag="ON550_002947"
     CDS             complement(1248..2408)
                     /locus_tag="ON550_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3955961.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVNNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKTAEPITALDAATMGGLMARLSKFNIEGVDDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(2408..>2538)
                     /locus_tag="ON550_002948"
     CDS             complement(2408..>2538)
                     /locus_tag="ON550_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3955962.1"
                     /translation="GTEAKKEQSVEEILNDVEEKRAEKIAAFTAALNKTFGQGDAK"
BASE COUNT          727 a          535 c          384 g          892 t
ORIGIN      
        1 gtttgcgtta aaatctttaa ccaaccatcg ttgaacgttt cttcgagtct acgattattc
       61 gccattccca tcacctgcaa tatattcttg tagcgcataa tgttgaatga aacctaataa
      121 ctcgctagcg aaattttgtt caaactcacc taaagcacga ttccagtcgt atctacatct
      181 ttcgattagc aatccgtatt ctaagctatc aggagaaaaa gaaagagttg tactcacttt
      241 actttgtaaa taaacagcat ttttagctat catcttttta attgattcat cttcttcgtt
      301 ccaggtaacg taaatattat ccttcacagc tattagcaat tcttcagtca cttgttcagg
      361 cgtcatctaa ccaccgcctt aattgcttta gcatatgcgt aagagcattt tttcttgttt
      421 acaaatgata aatcttcatc aaaaggcgtg gaagtcacgt atctcccttt gaaaaataaa
      481 tcttcatcat ttgttgttac tccagcgtta tgcaagattt ttacttcttt aaatttttct
      541 attggatcag tagcaaaaca aaagtttaat tcctcgtgaa ctttaggacc aatattgaaa
      601 tacatcatgt tccaaagctg tgcccacatc tcggctgtcc aaatctgtac atttgttttt
      661 tgccctctaa ggtagcgata tagccgatta gaatctagat aaaccttttt ccaataattc
      721 gctttaggaa ggttaataat ccaccgtgcg cctcctgaat tagtgtttat agtttccaaa
      781 gattctactg taacatttac aatatttgcc atatctttta gaatattttc tccgttttca
      841 cagcttctaa tataatcaag acttagataa ctacagcagt cgctacaata ccaaacatca
      901 tctttagaag gcaatttgcg caaattaatt cttttattga aaatgacatc cgaatcgata
      961 tagaaatatc ggtcgtcctc acgcgaatga tcttcttcta aatatttcca ccataaatat
     1021 ggtttaatcg aaggaatata ctctttgtcg tcccgcagat catcgtacac atgaacttca
     1081 acgccatatt ccttctcaaa aaaaatagga atctgatcat cgtgtctgct gaaaagcaat
     1141 acgatatctt tgattcctag tttcttcaga ttagttaaac aaacttcaag ctcccattta
     1201 aaccgattga ttgccggctg acaaagaata tacttcattc tgatcaccta cgcttgtgtt
     1261 gtagttgttg tggttgttgg ttttgtagtt gtagtagtag ttcccaaagc gctaatatct
     1321 aatacaatga aactatcgtt acgtttaggt tgaccgtttg catattgttt agctagataa
     1381 atgcgttcgt cttcaacaaa atggtattca tctgaagctt caatttttag tgtagatcct
     1441 acacccataa agtaatctga agctacccca ataactgctt ttccttctgg cacagcagtt
     1501 gactgcaagt ctgaaactgg tactggtaat acttgtacgt attcaccgtt agcagttagt
     1561 acagtcttag ctgggaatac tttagaccag taatcagttg gattcacaat taggaccaca
     1621 tcagaaggat tcacattacg ataaatcgga tcatctacac cttcgatatt gaattttgat
     1681 agtcgtgcca tcaaaccgcc catagttgca gcatctaaag ctgtaatagg ttctgctgtt
     1741 ttttcagcat attctccgct agtttgtttg ctcatgtcgc gcatcattcc gactggcata
     1801 tctttaccag taccatcaac aattgcttgt tctaatgcaa ttctcaatga ttcaactaaa
     1861 acagtacgaa cataacgatc taaccatact ggacctaagt caagcattgc cttacataca
     1921 ggaatataac ctgatagctt gaactgcttc atgttaatta cgtcaaagcc attatctaaa
     1981 acttttttaa cagcttcgca aagtttgccc caccatgctg gattgactcc acgtgacacg
     2041 atccattctg ttacaccagt tgtgttaaca aaagtaattt tttgcaatag tggatgagat
     2101 tgttctagat cttcaaatac gcgttcaaat acagtagctg gtactaattc ttcaactcct
     2161 gcaaaacctt cattattcac tacttcgtta tagaattttg tttcttgtgt agttagtaca
     2221 cgctgcccac ggttcattaa tactaattga tcttgatttt ttgctgttgc ttcttctaaa
     2281 attttatcct gtatttcctt agataaactt accatagctg cgctaaaaga ctcttcgtta
     2341 ccatctttaa aagctttcat caattggtcg cttgcagctg ttacaccttt taaattttta
     2401 actgtcatta ttttgcatct ccttgtccaa atgttttatt taatgctgct gtaaatgcag
     2461 caattttttc tgctcttttt tcttcaacgt cattcaaaat ttcttcgacg ctttgttctt
     2521 tcttagcttc ggtacctg
//
LOCUS       DAJSVF010000189         2486 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSVF010000189 DAJSVF010000000
VERSION     DAJSVF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.199"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1016..1306)
                     /locus_tag="ON550_002949"
     CDS             complement(1016..1306)
                     /locus_tag="ON550_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955963.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2206)
                     /locus_tag="ON550_002950"
     CDS             complement(1514..2206)
                     /locus_tag="ON550_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3955964.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          735 a          459 c          349 g          943 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt tctacatttg
     1681 ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta ttttgtaatt
     1741 ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc agatgattga
     1801 tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt tgtttgcttt
     1861 gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct gctgccgatt
     1921 gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca tctgcctttg
     1981 aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta atcgcttgct
     2041 ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta tcaatatctg
     2101 tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag ccaattagac
     2161 atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc atagctcctt
     2221 aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca agcgttttta
     2281 aattacatat gtaattttaa tataaatact atcaaatact catttaatcc tcctttttta
     2341 tctatttaaa aaatcttttt tatagagaaa aatgcaggtt aatactttct taaataaata
     2401 tttatggtga acaaaaacaa aaaagcaagt tttattaaac gtaacccttt tcatttcata
     2461 gattactgta tttattacaa aaaacg
//
LOCUS       DAJSVF010000190         2477 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSVF010000190 DAJSVF010000000
VERSION     DAJSVF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.200"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..866)
                     /locus_tag="ON550_002951"
     CDS             complement(<1..866)
                     /locus_tag="ON550_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3955965.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGNVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYTFIMDYNTGKELARVRADGIY
                     RPDVNQGYNTSG"
     gene            complement(877..1074)
                     /locus_tag="ON550_002952"
     CDS             complement(877..1074)
                     /locus_tag="ON550_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3955966.1"
                     /translation="MILPDKYYQVIKWTVLTVLPATSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1090..1332)
                     /locus_tag="ON550_002953"
     CDS             complement(1090..1332)
                     /locus_tag="ON550_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCR3955967.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(1389..>2477)
                     /locus_tag="ON550_002954"
     CDS             complement(1389..>2477)
                     /locus_tag="ON550_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955968.1"
                     /translation="GFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTT
                     FPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGL
                     TDVWSLTFTPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRG
                     IGMRDSNNPKYYVWDLEPGYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASIN
                     EVDTTAKDAQTKANANETAIKSTNSRVNEIENIITALTKEETYLLPASIVSGGSLKFT
                     SRGGMTVATGSFKLAKDIAFGEVLVNDLPDSMINIDNNAIGTGAVTGNSVSQIYVESN
                     TRKVVANTAMKKDQWFTVGFSYHSKLSI"
BASE COUNT          657 a          535 c          416 g          869 t
ORIGIN      
        1 tttccagaag tattataacc ttgatttaca tctggtctat aaattccatc agctcttact
       61 cgagctaatt ctttcccagt attgtagtcc ataatgaaag tatactcgta tttatagtta
      121 gcaatgtgcc atccagccac atgcaagttt gcgttttcga tttctccaaa ctgatcaatg
      181 tgggcgtaat ttgttccatc tgttagtgta ggatttgcag cacctgctcg agttggatca
      241 attactggtt tatcctccga agtagttgga ttatcatccg taaaaccatg agccaaatca
      301 taggctagtt tttctttact tacacccatt tcagaaagat aaccgtaagg atctgtatga
      361 tcgccccaaa tattttgtgt tacccataaa tgcgatttga ttcctggttg gttataagga
      421 gtgtctaatg ttaatggaat accatatttc attgctgaat ctctagctaa ttcaacgtat
      481 gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc aatctgcacg
      541 ggactgttgg cattagcata cgaaccagcc ccatactgca cataaccagg ttgaccgact
      601 tgataaacat ttccgccgtc tcccacaatg taagcagtgt aagcactagt ccatgaacgt
      661 tgcatatact gtgcttcatt gcgtcctgtt gctgtttcgt tagctgtttc atgcagtaaa
      721 atatactgat tattcgctac ttgagagcta ccttcgtttg gccctaaatt aaattcattg
      781 ttaatcgtat aggcaaaccc attaattggc aataaaaaaa gagccgttaa taggctcatc
      841 gcagtaatag taattttctt tttcatttgt ttcctcctat tttttcaaat tataagccga
      901 cacaccagtg ataacaccta aaaatgttgc tgctgcattg atagtcagaa ctgccatatc
      961 tgttccattc catccatatg ctttgcctag tgttgcaact aatacagatg tagccggaag
     1021 taccgtaagt accgtccatt taatgacttg ataatactta tcgggtaaaa tcatttcttc
     1081 tcacctcctt tacaatttag tcaagaaata gccaatgatc gtaatgccta aaccgatcat
     1141 gtaaccccac gaccatttat tattggcttt catttctttg atatcttccg cattattaag
     1201 cgcaatagaa tatgcctgat ccgctctatc ttttgcactt tccgcttttt cgcgtaatga
     1261 ttcgtaatta tccaacttcg tttcaatacg cactaaacgt tctaccacgt cttgtattgc
     1321 ttcatctttc aaccaactag cctcctttca ttgcaaaata aaaacgtact cgaatgagta
     1381 cgctaaatct atatagataa tttactgtga tacgaaaatc caactgtaaa ccattgatct
     1441 tttttcatag cggtattcgc aaccaccttt ctcgtattag attcaacata tatttgcgat
     1501 acagaattac ctgtgacagc tccagtccct atagcattgt tatcaatatt aatcattgaa
     1561 tctggcaaat cattaactaa aacttctccg aatgctatgt ccttagccaa cttaaatgat
     1621 cctgtagcta ctgtcattcc accacgactt gtgaatttaa gagatccgcc acttactata
     1681 ctagcaggta gcaaatatgt ttcttctttt gttagggccg taataatgtt ttctatctca
     1741 ttaaccctac tattcgtact ctttatagca gtctcattag cgttcgcttt tgtttgagca
     1801 tccttagctg tggtatctac ttcattaatt gaagctgtca actgcgaatt aatctccgat
     1861 actttcccat cggtataatt gtttgcttta cctgtgattt cagcaacctt gatatctgta
     1921 gctaggttat tctcaacata gccaggttct agatcccata cataatattt tggattgttt
     1981 gagtctcgca tgccgattcc tcggtattta tattgctcaa tattcggggt tcgagtgtca
     2041 cctttttcaa tcttgagcca gtcaattcgg catgcgccgg ccgtttcttt aggcgactga
     2101 aaaatgcgaa ggtctttagg caaacccggt tcaagtttcg ttggtgtgaa tgttagagac
     2161 catacgtctg tcaatccctc aactggtttt agatctccaa aattaacgtt ccaataatta
     2221 tatgctacaa aggtttgact tgcgggtttt gttcctttaa gcgtgatagt atacgtttga
     2281 cctattataa gctcttcttc cgtgttacct ttatatattt cgtacgcgct agatttaatt
     2341 gggaacgtcg tacctttatt tgcaatattc ttacccaacg atgttttacc tacccagtaa
     2401 gggtcatcta gtaagtttgg ctgatacgga gtggctgttg agccttcttc gattttgatg
     2461 tcgtacaatt taaatcc
//
LOCUS       DAJSVF010000191         2471 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSVF010000191 DAJSVF010000000
VERSION     DAJSVF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.201"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(130..495)
                     /locus_tag="ON550_002955"
     CDS             complement(130..495)
                     /locus_tag="ON550_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955969.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            703..1185
                     /locus_tag="ON550_002956"
     CDS             703..1185
                     /locus_tag="ON550_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955970.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1382..2272
                     /locus_tag="ON550_002957"
     CDS             1382..2272
                     /locus_tag="ON550_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCR3955971.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          855 a          397 c          493 g          726 t
ORIGIN      
        1 tttttagtgt ttggagagaa gaaaagaagt aaaaaataga aaaactagtg atagttttgg
       61 tgatataaga aaaacatata tgtataaaaa gaagaaagta gcttctcttt gtacatatat
      121 ggtttgtttt tatggatgcc gagctttttt ataagataag gctaccgccc ataacgaccc
      181 aaggatttgt ggaactaagt aaatacctgc aagaagcaag atcagctggg tcggttttaa
      241 aggatgatag aagtaacgcc attcagtaaa attggcgcaa aataaatagg aagtgacagt
      301 agatataata ttggcaatga ataaagatat aatacgcctg ttcctttgaa aataatatcc
      361 agcaaaaaga gataaaaata tcaatgtgat gaacccgctt acggaatgat aacgaaaatc
      421 aagcagtaag gcgaaatgca cataaggaac aaaagataaa agcaacggaa ggtttttcca
      481 gcaagttttt atcatctttc atcaccacct aacaagtgtt attattttgt tagttttatt
      541 gtaaattgaa tcctgcaata atcaattact ttttcattat ttctagatta aatgaataaa
      601 ctctcatgcc attatctcta aaaaaaaagt ttttccccct aattttttta gatagaaaat
      661 tgtatagtat gatgagagaa aaagtagttt gggggaacgt taatgagaag aaaagcagta
      721 atcgatcggc tttatttatt tttttggttt tttcctatcg ttttgctacc ttttacatgg
      781 gtaaatgctg ttgtacaagg tgcggagata ttaggcctga tccaaggaag cggatttgtg
      841 gtttctagga attttccagc tttgattgtt ttctatttag tgataagtgt aggtctctta
      901 ttaccaaaat ctctgatcaa tcactacaat tggcagctgc tttacttttc ttgcttatta
      961 gtatttacat ctatgttttc tttgctgcct cgcttgatta tcggccctaa tggtttgagc
     1021 gagagtggat tgaaggattt agcttattcc tccatgtatt tcttgacgat caaaccatgg
     1081 ttctatttag taattgcctc atttattctt gcaacatttt tttacttctg gacagaaatc
     1141 aggataaaaa gtgaacgatc tgtatctata aaaaataact attgatggtg gatcatcagt
     1201 aaaaaaacat atgagctgcg taaaatgcat gtttctcata tgcttttttt attattatgt
     1261 aaagaatgcg aaaaacgttt acatgatttt tacataactt aactatattt ttacagcaac
     1321 catacgaggg atttacagtc gactgataaa ctttagttgt aaataagaaa ggagcaataa
     1381 aatgaaaaaa gtattgttta ctctaggtat gatgggaatt cttttagccg gatgcggctc
     1441 aggatcagga aatacagatg gctctgccac acaaaataat tcaagtgact caaatgaaca
     1501 agtaaaaatc gttgcggtag gttcgacagc attacagccg ttagtcgatg cagcacaaga
     1561 aagctttgta caagaaaatc caaattatca aatctctgta caaggaggag gaagcggaac
     1621 tggtttgagc caagtcgaag ctggagcagt tactatcgga aactctgatg tatttgcaga
     1681 agaaagagac ggagttgatg cttcaaaatt agtggatcat aaagtagcag tggtcgggat
     1741 ggcaccaatc gtcaataaag atacagatgt caaagatatc accaaacagg aattgattga
     1801 tatctttaca ggaaaaatca ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa
     1861 cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga
     1921 tggtgctacc cctgtgcagt cccaagaaca agattcatca ggaacagttc ggcagctggt
     1981 aagtcaaaca ccaggagcaa tcagctattt agctttctca tatctcgatg attccacaca
     2041 agcactaagt atcgacggtg tagaaccaaa agaagagaat gtagcagata acagctgggg
     2101 aatctggtca tatgaacata tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt
     2161 cttagactac atgatgacag aagaaatcca agaaggacct gtcaaagaat tgggctattt
     2221 accaatcaca atgatggaag tagaacgtga tcacgaagga aatatcaaat aactagcaga
     2281 taaacatgcc acggtagtcg ttactattaa ggaaaaatca actatgtttc cccacatata
     2341 catttttcct ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac
     2401 cgagggtcag ctgattgaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc
     2461 gtaacttttt t
//
LOCUS       DAJSVF010000192         2456 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSVF010000192 DAJSVF010000000
VERSION     DAJSVF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.202"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            2..1147
                     /locus_tag="ON550_002958"
     CDS             2..1147
                     /locus_tag="ON550_002958"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR3955972.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="ON550_002959"
     CDS             1318..1776
                     /locus_tag="ON550_002959"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR3955973.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="ON550_002960"
     CDS             1935..2405
                     /locus_tag="ON550_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR3955974.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          820 a          460 c          459 g          717 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaataaa aaaagatgaa ctatttctag ttcatctttt tgcctt
//
LOCUS       DAJSVF010000193         2401 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSVF010000193 DAJSVF010000000
VERSION     DAJSVF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.203"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            208..2070
                     /locus_tag="ON550_002961"
     CDS             208..2070
                     /locus_tag="ON550_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3955975.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2401
                     /locus_tag="ON550_002962"
     CDS             2070..>2401
                     /locus_tag="ON550_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3955976.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2401 a
//
LOCUS       DAJSVF010000194         2387 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSVF010000194 DAJSVF010000000
VERSION     DAJSVF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.204"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(4..249)
                     /locus_tag="ON550_002963"
     CDS             complement(4..249)
                     /locus_tag="ON550_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955977.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ON550_002964"
     CDS             complement(266..1117)
                     /locus_tag="ON550_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3955978.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ON550_002965"
     CDS             1283..2287
                     /locus_tag="ON550_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR3955979.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          762 a          403 c          482 g          740 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DAJSVF010000195         2358 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSVF010000195 DAJSVF010000000
VERSION     DAJSVF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.205"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..669)
                     /locus_tag="ON550_002966"
     CDS             complement(<1..669)
                     /locus_tag="ON550_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1062 family transposase"
                     /protein_id="HCR3955980.1"
                     /translation="MTYTHLTSNKLAMIEAYYNNHQSVAKTAVLLNRSRQTIHKVYQF
                     FKTGHNALDYFNQYKKNKTRCGRRPIVLSDKQTEYIQKRVVQGWTPDVIVGRAEFSIS
                     CSMRTLYRMFKQGVFEVTHLPMKGKRKANGHKETRGKQSFRRSLRDRGNDYSKFNQEF
                     GHLEGDTIVGKKHKSAVITLVERLSKVIITLQPEGRRAIDIENRLNQWMQSVPKHLFK
                     SMTFR"
     gene            complement(751..2205)
                     /locus_tag="ON550_002967"
                     /pseudo
     CDS             complement(751..2205)
                     /locus_tag="ON550_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
BASE COUNT          765 a          485 c          371 g          737 t
ORIGIN      
        1 gcgaaaagtc attgatttga atagatgctt aggtactgat tgcatccatt ggttcaaacg
       61 attttcaata tctatagctc gtctgccttc tggctgaaga gtgatgatta ctttagataa
      121 tcgctcaacg agggtaataa cagcactttt gtgcttttta cccacaatag tatccccttc
      181 aaggtggcca aattcttgat tgaatttaga ataatcattt cctctgtcac gaagtgatcg
      241 acgaaaagat tgttttcccc gagtttcttt gtgtccgttg gctttacgtt ttcctttcat
      301 aggtaggtga gtcacttcaa atacgccttg tttaaacata cgataaagtg tacgcataga
      361 acaagaaata gaaaactctg cacggccaac aatcacatca ggtgtccaac cttgaacaac
      421 cctcttttga atatattctg tttgtttatc tgataagaca ataggacgtc tgccacagcg
      481 agttttattc ttcttgtatt gattgaaata atctagagca ttgtgccctg ttttgaaaaa
      541 ttggtaaact ttatgaatcg tttgtctaga tctattcaat agtactgcgg ttttggctac
      601 agattgatga ttattataat acgcctctat cattgcaagt ttatttgatg taagatgggt
      661 ataggtcatt tgtcttcact ccttataatt cttttgcggg aactatcttg agtgtagcat
      721 aaatgacttt tttatttgtc tagcttaatt ttacaatcgg cgattgtcac ttgcaatatt
      781 tactggaatt tcgctcgtat cttcttttac ttcctcagtc tcttcatcaa tacctaaaat
      841 gaatgaagca ataaaaccga caacaaatgc aatcgcacaa ccaatcgctg cgtaaataaa
      901 atttgatagt cctttttccc caaagaaact tggtaacgca agaatgccag gagcaacttg
      961 agcatatcga ccgacaccca tgattccaat aaatagtccc gctgctccac cacctaccat
     1021 tgaagcaatt aacggctttt tgaatcgcat attaattccg tataaagctg gttcagtaat
     1081 tcccataaaa gcagacagac ctgaagaaac agctaattgt ttgacagaaa gttgttttgt
     1141 tctaaaggca actgctaagg cagcaccacc ctgagcaata ttggaaacca tcatacctgg
     1201 accagcaaca gtatcaaatc cagatgttac taacatatta ataccgattg gaattaaacc
     1261 gtaatgcata cctgtcatca caagtaatgg agtaaatgca cccacaagta atggaaccaa
     1321 ccaacttgca tacgtattaa caaattcaat aagtgaccct aacccattac ctaagaatgt
     1381 acccaaagga ccaatcacaa ttaaggtaat tggtgcaaca atcaacaatg ttaacagagg
     1441 cgtcataaat attcgaacag cattgggcat caatttatcg ataatcggct cgatatatga
     1501 cataaatcaa atagctaaaa taatcggaat aacagaagaa ccatagttag ctaacgtcac
     1561 aggtaatccg aataaagcta aggactcacc tgtttctcta gcacccgcaa tcattgctgt
     1621 aaaagtcgga tgtaacaaaa ttgctcctaa ggtaattgca atgtactgat tacatccgaa
     1681 tttttttgca gcagaactag ctaataagac cggtaaaaaa taaaaggctg catcaccaat
     1741 gaaatttaaa aattgatagg tttgcgattc tggtgtcatc cattccaaca tgactaatag
     1801 agctaataat gcttttaaca tccctgcacc tgttaatgcc ggaacaattg gaacaaaaat
     1861 acccgcaatt gtatctaaaa cttttgatac aatcccttta ttgtttgctt ctgaactact
     1921 ttctttagaa gcggaaaaat ctcctagttt atcgatttcg gcaaatacaa attttacatc
     1981 tccaccaata atgacttgaa attgaccacc ctgattaact acacctacaa cacctttaag
     2041 atttctaata gctgattcat ctgctttact gtcatctttt aaattaaatc gaagtcgtgt
     2101 tgcacaatgc gctaaactgc ttacattttc agaaccacca acatttgcta atatgtcttt
     2161 tcctaattct ttatacccca tgctactttc ctccttagag aacattttct cttttgataa
     2221 tttgaatata gcgtgctatt aatagataaa caatacattt gaaaccgttt tacacagcgt
     2281 tgcgaaattc attcagttta cgattatttt ttcgcaacac tgttccaaaa aaataaaatc
     2341 cacttactaa ctaatcgt
//
LOCUS       DAJSVF010000196         2354 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSVF010000196 DAJSVF010000000
VERSION     DAJSVF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.206"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            66..2219
                     /locus_tag="ON550_002968"
     CDS             66..2219
                     /locus_tag="ON550_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR3955981.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
BASE COUNT          805 a          416 c          525 g          608 t
ORIGIN      
        1 tcttttttat taagaagttt ttgggtacaa tgaagcatga atgtaaatag gcggaaggat
       61 aggaaatgat gaaagatttt atgaaaaaaa tattaaatac ttcttggtta gaaaaaataa
      121 gaaaaggtgc acaagaacct aagaacaaaa cgtttcgccg gtcccatgtt ccttttcgct
      181 taaattttct ttttttcatt atatttacgt tatttgttgc gttgattgct agattgggtt
      241 atttgcagat cgtgaacggc gaagagatgg aggcgcgagt gaagtctact tctacgatta
      301 cggtccaaga aagtactcct cgcggaatga tctacgactc cacaggaaaa gctttagtta
      361 cgaataaagc gaatcaggcc atcacatata cgagaggaac acaagttacg gctaaagatt
      421 tgcttgaaat cgcaacgaag ctgaactcgc tgatcgatgt tccggtagat gaaaatctga
      481 cagatagaga taaaaaagat ttttggctgg ctgatcccaa acatttgaaa gaagccacaa
      541 aaagactttc ggcaaaagaa aagcaattag ggaacagcga acaatatagt gcgatggtca
      601 ataaagtcac agctgatgag attaatttaa acgaggagca attaaaaatt gctaccatct
      661 ttaaacggat gaatagtgca tatgaattaa acactgtcta tatcaaaaac tctggtgtta
      721 ctgatcaaga gttagctgtt gtggcagaac gcgcttctga acttccagga gtttcgactg
      781 gcacagactg gacacgagaa tataatgcag caagttcact gaaaagtatt cttggcagtg
      841 tgacgactga aaagcaagga cttccagcag atgaagcaga gaaataccta gcaaaaggtt
      901 attctcggaa tgaccgtgta ggacaaagtt atttggaaaa acaatatgaa gatgtcctgc
      961 aaggaaccaa aacccaatat gaagtttccc ttgataatga aggaaatgtt tcgaatcaaa
     1021 aagaaatctt ctctggggaa aaaggttcta atttaatgtt atctatgaat gcagaattcc
     1081 aatcaaaagt agaagaaatt ttgaaaagga attatcaaac attgatcaac aacggcaaag
     1141 cacagtattc gcctggtgct tatgcagtgg ctatgaatcc tcagacagga gaagtgctag
     1201 caatgactgg tttcagtcat gaacaaggtt ccaaagaaat caccgagaat gctttaggca
     1261 cgattactag cgcctttgct ccagggtctg tagtcaaggc tggtactttg acagcaggct
     1321 gggcaagtaa cgcgatcagc ggaaatcaag ttttgattga tgagccgatt cgtctgcaag
     1381 gtgcttcaga aaaaagctct gtctttaatc gaagcggaca agttgctctt gatgcagtca
     1441 aagcattgga attgtcttct aatacgtata tgatcaaagt ggcgttaaag atgttaggac
     1501 tagactatac acctggaatg ggactaccta gtttagatga agaagctaaa gcttatcagc
     1561 aattgagaga ctctttcaaa gagtttggtc ttggtacaac gactgggatc gatttgccga
     1621 atgaatctcc tgggatctca cgttctgttg attatatgaa gaagttcaat gcggataatg
     1681 gcaaagaatg gtatactcca ggtaacttta ccgatttggc atttggacag tttgacacat
     1741 atacaccaat ccaattagct caatatgctt caacagtcgc taatggcggg aaacgagtac
     1801 agccgcacct agtcaaagcg atctatggca atgacgaaaa tggaaacttg ggtgaagtga
     1861 agaaagaaat tggcacaaca gtagaaaata cggtcaatat ttctgcagaa aatatggcga
     1921 tcctccgtga aggattccac caagttgtcc atggtaccga tccttacaca gcagccaaac
     1981 cattagccag tgcgaaaatg gacctttccg caaaaacagg gactgcagaa acggttgcgg
     2041 aaggtcatcc agatattacg acagtcaata gtaatatcgt ggcatacgga ccaacagaca
     2101 atccggaaat tgccatcagt gttgtcttgc cgaacttatt agacgaacaa gaccacatga
     2161 acttgacgat tgctaaagaa ataatggacg cgtattatga tatgttcatg gcaaaataaa
     2221 aaagaggtcg tgaaaaagcc gcttagtttc gagtatgaag aaaaattttg tgaaaatagc
     2281 ttctcacatt tttgcttaaa tttgacttca taccgagaaa ctggcttttg aacaccgttt
     2341 actcggcaaa aaag
//
LOCUS       DAJSVF010000197         2352 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSVF010000197 DAJSVF010000000
VERSION     DAJSVF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.207"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(123..404)
                     /locus_tag="ON550_002969"
                     /pseudo
     CDS             complement(123..404)
                     /locus_tag="ON550_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            866..2248
                     /locus_tag="ON550_002970"
                     /pseudo
     CDS             866..2248
                     /locus_tag="ON550_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          856 a          401 c          390 g          705 t
ORIGIN      
        1 cgacaataat gacgtctttt ttgaattgtt tgcctttaaa atgattcatt actctgccct
       61 ctctgtcttt tttctcaatt ttacactaaa atagattttt tggaaaactt tgcaacagaa
      121 ccgaaatgag tatagtatga attagtaatt tggttttcat ctaagtttag ctttgattta
      181 ctaaaattga tatctcgata catgttcaat acatctaata caaatcgaga atcttctttc
      241 ggaaatggct cagaaagatc actccaaatt tcatcatcat aatgatactc gtaaccttct
      301 ctcaatattt cgatttttct ttcataataa gatctgtttt ggtcctcaaa tttagccaat
      361 aactcaaatt gatttactag tgacaatctc tgaaaactgt ccattaaatg tttcttccta
      421 tcttgttcat taacaaaaaa ttataagccc ttacaacgaa tattatatat taaatttaat
      481 tgtaggtgaa cacgaatttt ataaatattt atggtgttaa tgacaaatga tatccgactc
      541 ctcttcatac gttctggact atttgctaat agattaaaat ttcagcatct atttctgacc
      601 ataaataaat gagctgccga aaagctgcca aatcagactt gaaaataaag gatcaaaaac
      661 ttttaagttt ctgaccccgt aactcacact tcaaaatctt acttaaaaag gagtctgaaa
      721 gaaaaaaaga gtattcaaaa tttttttcta actagctaca tcacatctat gcattgacac
      781 tcaaagaacc agaataagtc atcagaaagc aagggggtgc ggacataatc ctggacgaaa
      841 aaataaactt aggaggaatg tccgtgtgtt ttcccacaaa gagcgtgtaa gggcaattca
      901 gttattttta aaatacgatt gctcttatgc tgcaaccatt cgagaattag ggtatccgtc
      961 gattggtgca ctacggaaat ggtataaaga gtatttggaa tccggagagc tccatcagga
     1021 gcaccgaaaa aaatcaaagt attctgaaga acaaaaacga atagcggtga atcattattt
     1081 tgaatatggt caatgttatg cacgaaccat tcgcatgctt ggttatccta atagagagtc
     1141 cttgcggcag cggtgtgaag aacttgcacc aggagctaga aaactccgaa aaagtgcggt
     1201 aaagttaaca caagatcaaa aagaagctgt gttgaaaaaa ttctataaac cgcaaactaa
     1261 taggaaaaat ttagccgaat ctggaggaat tagtagagtt actctatatc aatggaaaaa
     1321 cacggttctt ggaaaggatt ttccattgtg tatgacttta aaaaatcagg aaaaacaaaa
     1381 agagctactt ttaaaggaag tagaagactt acaaaaacaa gtacaccaat tacagctcga
     1441 aaaagcatta ctcgaaggag cggctgaact gctaaaaaaa gaaaaaggcg tcaatcttct
     1501 ttgtctatca aatcaagaaa aaacgatatt gattgatgcc ttacgaaatc agttcacctt
     1561 gaaagagctt cttcaacagc tccagtttcc taaaagcagc tatttttatc aaaaacaagc
     1621 acttgagaaa ccagataagt attacaaaga acgtcaaatt aattatcacg attttcaatc
     1681 ataacttctg tgcgtatggt taccgtagaa ttcaccaagc tttaaaaaat atgggtaaaa
     1741 agctttctga aaaagttgta cgtagattga tgactgagga aaatctattt gttaaatttt
     1801 ctagacgaaa aaagtattct tcgtatgctg gggaaatttc tcctgcacac cccaatttat
     1861 taaatcggaa ttttaaagca aagaagccaa atgaaaaatg gttaacagat attactgaat
     1921 ttagaattct cgcagggaaa atctacttat caccacttgt cgattgttac gatggtgcta
     1981 ttgttagttg gactattgga acaaagccaa cttgcttcac tctatgtcta aaaagagtta
     2041 ttcaccagat aattcagcgt gcgaaggttt ctttggacga ttaaaaaacg agatgtttta
     2101 tcaacgtgac tggaagaata caacaatcaa tcagtttata aacaaattgg atgattatat
     2161 tcattggtat aataatcaac gaattaagtt atctttagga aggctaagcc ctcttcaata
     2221 tcggaagaag caaagatata tttgttaaaa aggtccaaaa aaaatatccg cacccccttt
     2281 tttttgtaaa aaactattgc acaatacaag tagcctacgg ctatcgaaat ttccataact
     2341 ttcgagcaag aa
//
LOCUS       DAJSVF010000198         2192 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSVF010000198 DAJSVF010000000
VERSION     DAJSVF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.208"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..425)
                     /locus_tag="ON550_002971"
     CDS             complement(<1..425)
                     /locus_tag="ON550_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3955982.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="ON550_002972"
     CDS             complement(534..1565)
                     /locus_tag="ON550_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3955983.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          692 a          347 c          367 g          786 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttattg
     2041 atggattgga taaattaaac catcacaacg agtggaaatt atatgaaatt tttcataaaa
     2101 attcatatag aaacaaatga cattttatta gaatatgact ttcattaaca taaatgtttt
     2161 caaatttcaa ttaaattgtt ttaatcaaat tc
//
LOCUS       DAJSVF010000199         1981 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSVF010000199 DAJSVF010000000
VERSION     DAJSVF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.209"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..787
                     /locus_tag="ON550_002973"
     CDS             <1..787
                     /locus_tag="ON550_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HCR3955984.1"
                     /translation="KSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKV
                     REENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVI
                     SDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINL
                     AREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEV
                     RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            844..1236
                     /locus_tag="ON550_002974"
     CDS             844..1236
                     /locus_tag="ON550_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3955985.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1237..>1981
                     /gene="aac(6')-Ie"
                     /locus_tag="ON550_002975"
     CDS             1237..>1981
                     /gene="aac(6')-Ie"
                     /locus_tag="ON550_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCR3955986.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLN"
BASE COUNT          771 a          245 c          348 g          617 t
ORIGIN      
        1 taaatccgtc tctaagtcct tcgtttctag cttaacagaa cagctagaac ctatggttaa
       61 cgagtggcag aatcgtttat tatcagaaaa aaattatcct tacttaatga ccgatgtact
      121 ctatataaaa gtacgagaag aaaatcgagt actctcaaaa agctgtcata tagcgattgg
      181 aataaccaaa gatggcgacc gtgaaattat cggcttcatg attcaaagtg gcgaaagcga
      241 agagacctgg acaacatttt ttgaatacct aaaagaacgc ggtttacaag gtacggaact
      301 cgttatttct gatgcgcaca aaggattagt ctctgccatt agaaaatcct tcaccaacgt
      361 aagttggcaa agatgccaag ttcacttcct aagaaatatc tttaccacca ttcctaaaaa
      421 aaattcaaaa tctttcagag aagctgttaa aggaattttt aagttcacag atattaactt
      481 agcgcgtgag gctaaaaatc gattgattca tgattatatc gatcaaccaa aatattcaaa
      541 agcttgcgca tcattggatg atggattcga agacgccttt caatataccg tacaaggaaa
      601 ttcccacaat cgactaaaga gtaccaatct aattgaacga ctgaatcaag aagtacgcag
      661 aagagaaaag attattcgca tcttccccaa tcaaacatca gccaatcgct taattggagc
      721 cgttcttatg gacctacatg atgaatggat ttattcttca agaaaataca tcaattttga
      781 taagtagaaa tggtaaaaac attgtatagc attttacaca ggagtctgga cttgactgag
      841 tttatggaag aagttttaat tgatgataat atggtttttg atattgataa tttaaaagga
      901 tttcttaatg ataccagttc atttgggttt atagctaaag aaaataataa aattatagga
      961 tttgcatatt gctatacact tttaagacct gatggaaaaa caatgtttta tttacactca
     1021 ataggaatgt tacctaacta tcaagacaaa ggttatggtt caaaattatt atcttttatt
     1081 aaggaatatt ctaaagagat tggttgttct gaaatgtttt taataactga taaaggtaat
     1141 cctagagctt gccatgtata tgaaaaatta ggtggtaaaa atgattataa agatgaaata
     1201 gtatatgtat atgattatga aaaaggtgat aaataaatga atatagttga aaatgaaata
     1261 tgtataagaa ctttaataga tgatgatttt cctttgatgt taaaatggtt aactgatgaa
     1321 agagtattag aattttatgg tggtagagat aaaaaatata cattagaatc attaaaaaaa
     1381 cattatacag agccttggga agatgaagtt tttagagtaa ttattgaata taacaatgtt
     1441 cctattggat atggacaaat atataaaatg tatgatgagt tatatactga ttatcattat
     1501 ccaaaaactg atgagatagt ctatggtatg gatcaattta taggagagcc aaattattgg
     1561 agtaaaggaa ttggtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga
     1621 aatgctaatg cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac
     1681 caaaaatctg gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa
     1741 aaagaagatt gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca
     1801 atgaaatatt taattgagca ttactttgat aatttcaaag tagatagtat tgaaataatc
     1861 ggtagtggtt atgatagtgt ggcatattta gttaataatg aatacatttt taaaacaaaa
     1921 tttagtacta ataagaaaaa aggttatgca aaagaaaaag caatatataa ttttttaaat
     1981 a
//
LOCUS       DAJSVF010000200         1970 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSVF010000200 DAJSVF010000000
VERSION     DAJSVF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.211"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(493..1785)
                     /locus_tag="ON550_002976"
     CDS             complement(493..1785)
                     /locus_tag="ON550_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3955987.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          621 a          387 c          322 g          640 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca
//
LOCUS       DAJSVF010000201         1940 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSVF010000201 DAJSVF010000000
VERSION     DAJSVF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.212"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..211
                     /locus_tag="ON550_002977"
     CDS             <1..211
                     /locus_tag="ON550_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3955988.1"
                     /translation="NVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIGK
                     VLGITRQGATNLKHRLYKKIQKMLV"
     gene            309..500
                     /locus_tag="ON550_002978"
     CDS             309..500
                     /locus_tag="ON550_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3955989.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1014..1346
                     /locus_tag="ON550_002979"
     CDS             1014..1346
                     /locus_tag="ON550_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3955990.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1256..1459
                     /locus_tag="ON550_002980"
     CDS             1256..1459
                     /locus_tag="ON550_002980"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3955991.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1643..1798
                     /locus_tag="ON550_002981"
     CDS             1643..1798
                     /locus_tag="ON550_002981"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR3955992.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          712 a          319 c          329 g          580 t
ORIGIN      
        1 taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa tccttccttt
       61 gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata aaactgataa
      121 agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa aacatcgact
      181 ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat tttgtatgct
      241 tctttgctcg tcctcctcct tcccaaaaaa ataaatagat tataagttca tgggaagaag
      301 gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga aatgaagaag
      361 cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc tggagaactg
      421 gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta gctatctcga
      481 aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa aaagatagtt
      541 tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt atcgatttta
      601 cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt aacgtttttt
      661 aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa ttactttatt
      721 tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt caacgaggac
      781 ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta gatttattca
      841 actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat tttggttgtt
      901 aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac taagttgggc
      961 tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact ctattgaaaa
     1021 aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag aaaaaagtaa
     1081 ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc cgatgggttt
     1141 cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga ttacctgata
     1201 aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa gacgagtgtt
     1261 ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt acgatgaagg
     1321 aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt tatgcacaag
     1381 aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag aaatggtaaa
     1441 aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg tgacggatat
     1501 tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg acttagcaac
     1561 tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg tcgtccgtcc
     1621 attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag gtagccagca
     1681 tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt caatgagcca
     1741 aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa tcaaatgaga
     1801 attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa tccaagatta
     1861 tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga ctccttgaca
     1921 atttgaagcg aagaaaataa
//
LOCUS       DAJSVF010000202         1926 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSVF010000202 DAJSVF010000000
VERSION     DAJSVF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.213"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..135
                     /locus_tag="ON550_002982"
     CDS             <1..135
                     /locus_tag="ON550_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3955993.1"
                     /translation="EMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK
                     "
     gene            136..888
                     /locus_tag="ON550_002983"
     CDS             136..888
                     /locus_tag="ON550_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3955994.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            893..1840
                     /locus_tag="ON550_002984"
     CDS             893..1840
                     /locus_tag="ON550_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR3955995.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          668 a          262 c          386 g          610 t
ORIGIN      
        1 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
       61 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
      121 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
      181 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
      241 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
      301 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
      361 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
      421 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
      481 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
      541 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
      601 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
      661 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
      721 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
      781 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
      841 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
      901 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
      961 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
     1021 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
     1081 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
     1141 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
     1201 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
     1261 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
     1321 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
     1381 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
     1441 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
     1501 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
     1561 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
     1621 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
     1681 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
     1741 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
     1801 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
     1861 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
     1921 gataga
//
LOCUS       DAJSVF010000203         1909 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSVF010000203 DAJSVF010000000
VERSION     DAJSVF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.214"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            493..711
                     /locus_tag="ON550_002985"
                     /pseudo
     CDS             493..711
                     /locus_tag="ON550_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            971..1225
                     /locus_tag="ON550_002986"
     CDS             971..1225
                     /locus_tag="ON550_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3955996.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1226..1543
                     /locus_tag="ON550_002987"
     CDS             1226..1543
                     /locus_tag="ON550_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3955997.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          661 a          300 c          354 g          594 t
ORIGIN      
        1 tcattatatc accaaacccc atcagcgtgc aggctgatgg ggttttttta cgtttctata
       61 ttatagaatt aacgggtagg cttactgtct tttttggcaa ttttgcaatc aagccatttg
      121 cagaggtaac tgcacactac atctgcaaat ggcttgattg caaaaacaaa atcaaaaagg
      181 tatttcatta tatctcctcc tccccttgac agattaggcc ggggtagcag acagcaaaac
      241 tattataaca taactgaaat acctaaatga catatacaga tatgataatt tatgtattga
      301 tttttctaac aaaagcacat attttaatat atactaccta ttttaggtag tatatatttt
      361 tagatacaat atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctaaataga
      421 gttactttca atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt
      481 gatgaaattg ccattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat
      541 tggtctaatg gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa
      601 aataaagtta ttccagtatt gctatatgga acacataaca atttttatga attagccgag
      661 aacactcgca actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta
      721 tatcaatcaa ccgaccgaca tatgttaaat ctatatataa taaaaatacc tacttaaatg
      781 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
      841 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      901 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      961 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
     1021 ccttccaagc tctcttggat taaaaagcgg agacactatc caagccaaac aagaaggcaa
     1081 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
     1141 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
     1201 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
     1261 ccagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tgtctccagc
     1321 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
     1381 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
     1441 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
     1501 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggcaaaag
     1561 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
     1621 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
     1681 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
     1741 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1801 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1861 tgacgtaact tttaataaat actacctaaa taaggtagta tttattttt
//
LOCUS       DAJSVF010000204         1738 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSVF010000204 DAJSVF010000000
VERSION     DAJSVF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.215"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1..911)
                     /locus_tag="ON550_002988"
                     /pseudo
     CDS             complement(1..911)
                     /locus_tag="ON550_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1588..1661)
                     /locus_tag="ON550_002989"
     tRNA            complement(1588..1661)
                     /locus_tag="ON550_002989"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1625..1627),aa:Ile,seq:cat)
BASE COUNT          507 a          340 c          311 g          580 t
ORIGIN      
        1 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
       61 atcaaattcc tttccccagt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      121 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      181 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      241 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      301 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      361 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccgactgag
      421 ttgaagccca cgctttgcta aacacttctt caattttcgg gtaccgtaag cctttcggtt
      481 tcgaataaac tcttcctgaa cgatctcttc taagtcggac tcattttcga tcggtttcgc
      541 ttgataatag taggtttgac gagaaatatt tagaattttg cacatcgctg atatggaata
      601 tttatgcttg ttggcatcaa ttacttgtct tttcgtccga atatcagcgc cgcttgcttt
      661 aaaatatcat tctccatttc gagttgctta tttttctttc ttagtgcgat caattctgct
      721 tgttctggtg ttaagttgtc tctttctttg aatgagcccg ttgattgtgc ttgcttcatc
      781 catttatcga aagaagaagg cgtaagctca tactctcgaa taatttctgc gcgaggctta
      841 ccagcgagat agagatcgac gatttgttgt ttgaattctt ttgaataggt tcttcgttga
      901 cgtcttgaca tgaaaaatcc tccagtgtgt tttttattat tctacacacc ttattttttc
      961 tgtctagtct agtgtagccg attcatgact gacgtgccgg acgcacgtag cgatcatcgg
     1021 cgttatgact atataaagta tactttcgtt gatccagaaa ggtataaaga ttaaagtagg
     1081 ctacctaaaa aggtagcccg gaacgaattt tatcaccata cttgtgaaag gagatatttt
     1141 ttaagttagt attaagattg tgtaatatga tgatatctat attttatagt atcagtgcta
     1201 taaaatcaaa aataagtcac taattaacta ccactccaat tgtaagcctt ttttctcact
     1261 ttttttcaaa aatatggtat gctttttaat ggcttcaaat ataaaagagt ttaaagcgta
     1321 acacacttat ggggaagtgg tttggggtgc gctttaaact cttctttatt attatctcac
     1381 aatttaaccc aaatgtcttt ctatttaaaa atcaaagtaa aacttttcaa atatacagaa
     1441 gtataactat gtgaaacatc ctttcattaa tccataaaag gatacataaa aaagccactc
     1501 atttgagtgg caatgaagaa aagctttagc ttgtataata ctcttcaaaa aaattctaac
     1561 acagaacgat tcaaatggct acgttaatgt accctgtagg actcgaacct acgaccggac
     1621 ggttatgagc cgtctgctct gaccaactga gctaagggta ctggttgttg ccacataaag
     1681 ccataaacaa tcaaccagta gaatgtgtgg caacaaacct gttatcgcat agcttgga
//
LOCUS       DAJSVF010000205         1655 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSVF010000205 DAJSVF010000000
VERSION     DAJSVF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.216"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            4..>1655
                     /locus_tag="ON550_002990"
     CDS             4..>1655
                     /locus_tag="ON550_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955998.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGA"
BASE COUNT          681 a          267 c          366 g          341 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgta
//
LOCUS       DAJSVF010000206         1655 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSVF010000206 DAJSVF010000000
VERSION     DAJSVF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.217"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            4..>1655
                     /locus_tag="ON550_002991"
     CDS             4..>1655
                     /locus_tag="ON550_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3955999.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGA"
BASE COUNT          679 a          273 c          362 g          341 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgaa caaatcgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggcgga
      301 ctagatatga cgatcgaaaa tattccaaga atcagagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgt ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt aacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca catcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctctgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggagaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga caaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggatatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatcac tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaactcag ttaaaacctg aagactatcc gaaaatcttc
     1501 ccgaaccgtg tctttgattt tcaattggat caaacagggc agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tatcgggatt caaattcgag atattacgga gagtgaaatc
     1621 tatcaaagag aattaggaca agcacgtggg gcgta
//
LOCUS       DAJSVF010000207         1549 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSVF010000207 DAJSVF010000000
VERSION     DAJSVF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.218"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            36..842
                     /locus_tag="ON550_002992"
     CDS             36..842
                     /locus_tag="ON550_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956000.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKMKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            864..1094
                     /locus_tag="ON550_002993"
     CDS             864..1094
                     /locus_tag="ON550_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956001.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYLAAKKYFTADNK"
BASE COUNT          636 a          225 c          280 g          408 t
ORIGIN      
        1 agactttagt tatttcactc gttaggagga agaaaatgag taagaaaaaa ggagaaagac
       61 aacgtttcaa aaaacgaaaa agttatcttc aatggctggg aatctttatg attctcggct
      121 ttttgattct tgctagtccg atttacacgc atcagaaggt agacatacca gatgaagtaa
      181 gcacgaacgc atttttaaac acgaaggaac taacgatggt ttcaaaagaa aaaggagcga
      241 accagcaatt agtttttgaa tttgcaatcg acgatgaaga tcaaagctta ttaaaagaac
      301 tggctaatct taattatcgc gtagaagtaa agacagctaa aggggattat caagcaataa
      361 aagccaaagt gattaaggtg tctgatgatt attttgcagt gaagctatcg catgtaccag
      421 aagaatatat tgctatgcga ttaaccatta ttcctgaaaa aattgatcca aaggtggata
      481 tgcaagaacc acaagatctg atttattacg ttcacgaaga taaggttaaa gatcaagtga
      541 aagatgaaga ttatgaacaa catgcgatca attacaaaat gaaaggatac aagaaagaac
      601 aaaaagcagc tcaaaaacaa atggaatctt tccaagcaac cattgatttg aatgaagaat
      661 tagtgaagaa gctaaaagat caattgcctt atcaagtcgc agatgatcag gaaaatactg
      721 aaaacaagat taatagctac cagcaagaaa ttgaaacatc gaaaacgcaa atgaaagatc
      781 aagaagagat cattcaaaaa cttcaagaaa aaattgatcg tatgacaaaa gaaaagattt
      841 agaaggacag gtggagtgaa aaaatgaagc gaacaaactt ggctgacaaa gatactttcg
      901 ctgagcagaa agtattgatt aaagcctatc tcaaagttgt aaaagctaaa gaaatatgtc
      961 gagaagtgaa tttatctaat acaacaatat ctgagttgaa gaatggtcga aagaatttag
     1021 atactttaaa attcagatca tttgaagctt tgtatttagc tgctaaaaaa tattttacag
     1081 ctgataacaa ataaaaaaca ggagtatttt atgtgaaaaa agtatataaa aaatgcttat
     1141 gaatcagcta tatcacctta accatttgtt aatgagatgg gagaaaacat taaccacgat
     1201 tctaacgcaa acatcaagca cataaaacat ttaacactcc tcctttctgg ctgaagattt
     1261 caaccaaaca ggaagacagc gtatcaatta tagcatacac tttttcttaa attcattttt
     1321 catatgacaa aatatttcct tctaatcgac aaagttagaa ggatttttta ttgtctaaat
     1381 acacaaataa ccttaattta taaagagcat aaggaaacgt gtacccactt tcaaaataaa
     1441 gtgggtagat aggctgtgcc tatcacgttt ctgaaaaaaa taaatgaccc caaaattttc
     1501 aaattatgtg taggtttatg ggtagcaaaa atatgaatgt gggtagcac
//
LOCUS       DAJSVF010000208         1540 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSVF010000208 DAJSVF010000000
VERSION     DAJSVF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.219"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1490
                     /locus_tag="ON550_002994"
     CDS             <1..1490
                     /locus_tag="ON550_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3956002.1"
                     /translation="QQQTIQNLTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNL
                     FSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGS
                     QMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSL
                     GSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGER
                     LRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPK
                     TFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQ
                     LDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACK
                     LRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAE
                     LNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          520 a          280 c          318 g          422 t
ORIGIN      
        1 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
       61 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      121 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      181 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      241 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      301 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      361 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      421 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      481 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      541 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      601 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      661 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      721 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      781 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      841 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
      901 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
      961 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1021 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1081 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1141 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1201 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1261 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1321 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1381 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1441 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa aaacgccaaa
     1501 cattcccgat tctattctat aggtcgggtt atttgacgta
//
LOCUS       DAJSVF010000209         1532 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSVF010000209 DAJSVF010000000
VERSION     DAJSVF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.220"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..335
                     /locus_tag="ON550_002995"
     CDS             <1..335
                     /locus_tag="ON550_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956003.1"
                     /translation="QEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDA
                     AFIEDPQHNQVILKGKSYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQ
                     QLSQKERV"
     gene            396..>1532
                     /locus_tag="ON550_002996"
     CDS             396..>1532
                     /locus_tag="ON550_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3956004.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQL"
BASE COUNT          574 a          239 c          327 g          392 t
ORIGIN      
        1 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
       61 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
      121 atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt ttaaaaggaa
      181 aaagttatgg aaaagagatt caaacggcgt taccttcaga agaattttcg ctaggaaatc
      241 cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat gaaatctatt
      301 atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga ctatctgaaa
      361 aacggatagt cttttttctt ttgggaggtt tgaaaatgac cgaaaaaaaa atgagtctta
      421 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
      481 tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa
      541 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
      601 tttttttcat agacaaagag atccaagact ctaagcaaca atttaaagca gccacagatt
      661 ttattctaaa gaatgaagac aaaacagaac gagtagaaaa tcttcatttt gagacagaaa
      721 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
      781 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
      841 ccgaactaaa gccaaaacat gaacggcaaa atttcttagt tcgagacgat cgtttagatc
      901 atgcggtcgc ctttttatgg aaagatccac agacaaatga gacagtaggg gcgagttatc
      961 aaggaacgtt cattgattat gaacgttttg gtgagagagg cacatataag catatagata
     1021 aaaattcgac agcgaatcat ggctttaacc taaagatcgg tgaccctaag cagttaaaat
     1081 tttttgagag tagtattgat ttacttagct atgctgcact gaatcgtgat cagttaaacg
     1141 atacgtggtt agtttcgatg gaagggttaa aacaccatgt gatcagtcat tattttggtg
     1201 aggcagtatc tgaattgaga aagaaacaag cctttcctca atccatcgag atttgtgtgg
     1261 ataatgaccg tgcaggacat attttctacg aaaaagaaca attaatgggc gcagttgatc
     1321 catttacgaa tcagaaagta cgttgtgaac gaggaatcgc taatgattgg caagtgccaa
     1381 aagaatacaa agtcatttat gaagaagtcg caaaagaaga aaagatcaca ccagaagcta
     1441 ttatggccat acacaagaca gaaaacaatc tgcaattaac gaaccaattg gtttccgctc
     1501 acaaagtaaa tgcgtctttt ggtcagcagc tt
//
LOCUS       DAJSVF010000210         1528 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSVF010000210 DAJSVF010000000
VERSION     DAJSVF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.221"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            209..1075
                     /locus_tag="ON550_002997"
     CDS             209..1075
                     /locus_tag="ON550_002997"
                     /inference="COORDINATES: protein motif:HMM:NF025663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956005.1"
                     /translation="MNIHNIGKKILCRTFPIIMTKYFYRKILGRKLKLKKPETFNEKI
                     NWYKLYFCPQNDLVIQCTDKFRVREYVEDMYCGDSLNDLIGVWDGVDEINWNSLPQRF
                     VLKCNHGCGMNIICPDKTSLDIEETKNRLNKWMKMDWGKESIEPHYSKIKPKIICEKF
                     IENTLDKESSLPPDIKIHCFNGIPKVIAYYSGRGNDMHGTFYDVNWNKLDFATKEGSV
                     ISKPICLDKMLLYAEKLSNPFPYVRVDFYECNNIPVFGELTFTPASGRSPYMTREADE
                     YMGNLLNLKEME"
     gene            1077..>1528
                     /locus_tag="ON550_002998"
     CDS             1077..>1528
                     /locus_tag="ON550_002998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956006.1"
                     /translation="MRTKKAFYNTTTGIIYQLCSIVCGFIIPRLILGYFGSEYNGMIS
                     SITQFLGWISILTAGVGGVTRAALYKPLNKNDNYKISGIINATDNFMKKIALIFLGGL
                     IIFAIGYPFLLKNKFEWFFVFTMVLVLGINTFAQYFFGMTYQNLLEAD"
BASE COUNT          578 a          166 c          266 g          518 t
ORIGIN      
        1 ttaagtatgg acagatatat caacaatata ttattaatag agagtttaaa gcagcaaaaa
       61 agtttaaaaa tcaatatcat attaatatat tcacatcatt aaaagactta gttatccatg
      121 taataaaaag atactctatt gcgttgaaaa aaagaacatt gtcttatgaa gaaatgttgg
      181 tagaattaga aagaggaatg aattactaat gaatattcat aacataggaa aaaaaatact
      241 ttgcagaact tttcctatta tcatgacaaa gtatttttat agaaaaattc ttggaagaaa
      301 actgaagtta aaaaaacctg aaacatttaa tgaaaaaata aattggtaca agttatactt
      361 ttgtccacaa aatgatttag taatccaatg cactgataaa tttagagtaa gagagtatgt
      421 agaagatatg tattgcggtg attctctaaa tgatttaatt ggagtatggg atggagttga
      481 tgaaattaat tggaactctc taccacaacg ttttgtgtta aaatgtaatc acggatgcgg
      541 aatgaacatc atttgtcctg ataaaactag tttagatata gaagaaacaa agaatagatt
      601 aaataagtgg atgaaaatgg attggggaaa ggagtctata gaacctcatt attcaaaaat
      661 taagccaaaa attatttgtg agaaatttat tgaaaatact cttgataaag aatcttcttt
      721 accgccagat ataaaaatac attgttttaa tgggatacct aaagttattg cgtactactc
      781 aggtagaggt aatgatatgc atggaacatt ttatgatgtt aattggaata aattagattt
      841 tgctactaaa gaaggttcag tcataagtaa gcctatttgt ttagataaaa tgttattgta
      901 tgcagaaaaa ttatctaatc ctttccccta cgttagagta gatttttatg aatgtaataa
      961 tattcccgtt tttggagagt taacttttac acctgcaagt ggaagatctc cgtatatgac
     1021 gagagaggca gatgagtata tggggaattt gctaaattta aaggaaatgg aataaaatga
     1081 gaacgaagaa agctttttat aatacgacaa ctggaatcat atatcagtta tgttctattg
     1141 tatgtggttt tattattcca agactcattt taggatattt tggatccgaa tataatggta
     1201 tgatttcatc tataacccaa tttttaggat ggattagtat tttaactgca ggagttggag
     1261 gagtcactag agctgctctt tacaaaccat tgaataaaaa tgataattat aaaataagcg
     1321 gaataattaa cgcaactgat aattttatga aaaaaattgc tctcatattt ttggggggac
     1381 ttataatttt tgctattggt tatccatttc tcttaaaaaa caaatttgaa tggttttttg
     1441 ttttcacaat ggtattggtt ttgggaatta atacttttgc tcaatatttt tttggaatga
     1501 catatcaaaa tttattagaa gctgatca
//
LOCUS       DAJSVF010000211         1517 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSVF010000211 DAJSVF010000000
VERSION     DAJSVF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.222"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1028
                     /locus_tag="ON550_002999"
     CDS             <1..1028
                     /locus_tag="ON550_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR3956007.1"
                     /translation="IFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTS
                     PNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSF
                     LVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSID
                     RKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGET
                     DFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNI
                     FSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISY
                     LYLKKEN"
     gene            1030..>1517
                     /locus_tag="ON550_003000"
     CDS             1030..>1517
                     /locus_tag="ON550_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956008.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VY"
BASE COUNT          510 a          181 c          245 g          581 t
ORIGIN      
        1 taattttttt agctgcgcta atatctatct atcaatttat ttttttgatt tcttatcatg
       61 tcccaacagg aacgccagga ttacttgcaa gacaaggttt tattgaacat cggttatttg
      121 gtgtctatac aagtccaaat gttggagcta tgtttggata tacttctatt attttaagtt
      181 taattagtgt attaatttca aaaataacaa aaacgattcg tgttttttat atgatgaatt
      241 ttatcgtaca aatattttat attacacttt cttcgtctag aggtgcccag gtagtaattt
      301 gttctttttt agtattattt cttttgttgt ttgccaattc aaaatttaga aagagcatta
      361 gagaagtact acctgtaaaa atcaaatgga tggtaagcat attattagtt ctattggtat
      421 atttttcttt tggtactaat tatgctaagg attttttagc aacaattcct gtatcgattg
      481 atagaaaagt agaagttata gagtcaaatg aatcagataa aactccactt ccagaaaaag
      541 aagtaatatt acagcattct agtgataact ccgaaatttc agctggtcgt tttacgattt
      601 ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt tggttatgga gagaccgatt
      661 tctatgggaa tagcgatgca aaatttttga aaaaagtaga tcttgatact acagatgtga
      721 atgaactaaa aagagcacat ggaaacatgc ataatggatt tcttcaagta cttgtatcaa
      781 gtggtataat tgcttttata tgtatctatg ctttctatgt gttaaatatc tttagtttac
      841 tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct tggtattgtt ttaattttta
      901 tactatctat ttttatagat gaaatggtgg aggctcatgt acttttcaat aagcgcgatg
      961 tcatttcaat tgttttctgg tattatttgg gaagcatttc ttacttgtat ttaaaaaagg
     1021 agaattaata tgaagacagc tttcattgct tggactcctt tacatttgat taatatatta
     1081 aatgtttatc aaaattttta ccgagaatca aaaggagatt tatttattta ttctgaattt
     1141 gaaaacgcta gaagatatag tgagcgtata aaagaattag ggctatttaa tgatgttgtt
     1201 tttgtgaatc accggaaaat cggttcttcc tttaaacgta aaattaactt gttaactaac
     1261 cataatcaat ttttctcgga tgatttatcc gtttatgatg aaatttttat acagggggaa
     1321 aattatttca caaaaatact tttctccaat gtttacaaaa aaaatccaga tgtaaaacta
     1381 aattatatag aagatggttt gggtacttat ttgaagaaga gtatattttc aacaagtaaa
     1441 aacgttcaaa gagtttttaa aatattaaat aaaaatagca tcttcaaaaa aaaaattgac
     1501 gattattttg tctattg
//
LOCUS       DAJSVF010000212         1436 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSVF010000212 DAJSVF010000000
VERSION     DAJSVF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.223"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(942..1355)
                     /locus_tag="ON550_003001"
     CDS             complement(942..1355)
                     /locus_tag="ON550_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR3956009.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          530 a          237 c          211 g          458 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataa
//
LOCUS       DAJSVF010000213         1410 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSVF010000213 DAJSVF010000000
VERSION     DAJSVF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.224"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1410
                     /locus_tag="ON550_003002"
     CDS             <1..>1410
                     /locus_tag="ON550_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3956010.1"
                     /translation="IVAIGGLTAAFGILLPAILAVSYLFGPMMLIIGGIIAVIAGVIV
                     AIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKETWSGFVDWVQDIWQG
                     VSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEVWSIIWADIVGIVQI
                     PWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWN
                     GIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWNSIKNTITSLVQAAA
                     TVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWI
                     SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANI
                     WEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKW
                     HAKGGIFTKPTLLGGMNGVG"
BASE COUNT          432 a          208 c          329 g          441 t
ORIGIN      
        1 cattgttgct ataggtggtc ttacagcagc atttggcata ctgttaccgg caatactagc
       61 cgtgtcatat ttatttggtc cgatgatgct tataattgga ggaattatag ctgttatagc
      121 aggcgttatt gtggcaatta agaactgggg tgcaattact gactggttta gtggtttatg
      181 gaaaaaattt actgattggt tgggtgatac ttgggaaagt ataaaagacg gagcctcatc
      241 agtttgggat ggggttaaag aaacctggtc tggatttgta gattgggttc aagatatttg
      301 gcaaggagtt tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa
      361 catctggcaa ggagtccaag aaacttggca aacattcgtt gattgggttt caaatatttg
      421 gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat
      481 accatggaca ttaataacgt cattgattca agctggtatt aatattatcg tgggtatttt
      541 tgatgtagct ggacagttat tgggcgcagc ttggcaagct gtttggacac ctatttctga
      601 tttccttaaa aatacttggg atactatgac acaatggata agtatcgctt ggaatgggat
      661 tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac
      721 agctattagt actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat
      781 ttggaacagt atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca
      841 aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac
      901 agcatcaaat atctggaata gtgtgactag tagtataagc aatgctataa acgcagctaa
      961 aagtgccatt caaagtgttt ggaatagtat atcttcgtgg attagcggaa tttggaacgg
     1021 tatcaaaaat actgctttga atctttggaa tggaattaca agcactatta gctctaaagt
     1081 aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga
     1141 catattcaat aatgttaaaa gtacaattgc taacatatgg gaaggcatca aaaagactgt
     1201 tagcgctcca attgattgga ttagagacaa aatcagtggc atttttgata atttgaatat
     1261 ttctatacca catattccgt taccggaatt tatcatggaa ggcagcttta acccgctaaa
     1321 aggtcaaatc ccccatttgc gtgtaaaatg gcatgctaaa ggaggtatct ttactaaacc
     1381 aactttacta ggtggaatga acggtgtcgg
//
LOCUS       DAJSVF010000214         1398 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSVF010000214 DAJSVF010000000
VERSION     DAJSVF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.225"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1398
                     /locus_tag="ON550_003003"
     CDS             <1..>1398
                     /locus_tag="ON550_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3956011.1"
                     /translation="AVAIGGLTAAFTLLMPIIVGFMALGGPTTLIIGAVITVIAGVIA
                     IIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKETWSGFVDWVQDIWQG
                     VSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQI
                     PWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWVSIAWN
                     GIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWNSIKNTITSLVQAAA
                     TVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWI
                     SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANI
                     WEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDW
                     YAKGSVFNSPNIIGVG"
BASE COUNT          428 a          206 c          327 g          437 t
ORIGIN      
        1 tgccgtagcg attggcggat tgactgctgc atttacttta ttaatgccaa taatagttgg
       61 cttcatggct ctaggtggtc ctactacatt aataatagga gcagttatta ctgttattgc
      121 tggagttata gcaattataa aaaactgggg cgcaattact gactggttta agggaatatg
      181 gagtaaattc actgattggt tgggtggtac ttgggaaagt ataaaagaag gtgcctcatc
      241 agtttgggat ggagttaaag aaacctggtc tggatttgta gattgggttc aagatatttg
      301 gcaaggagtt tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa
      361 catctggcaa ggagtccaag agacttggca agcattcgtt gattgggttt caaatatttg
      421 gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat
      481 accatggacc ttaataacgt cattgattca agccggtatt aatattatcg tgggtatttt
      541 tgatgtagct ggacagttat taggcgcagc ttggcaagct gtttggacac ctatttctga
      601 tttccttaaa aacacttggg atactatgac acaatgggta agcatcgctt ggaatggaat
      661 tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac
      721 agctattagt actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat
      781 ttggaacagt atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca
      841 aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac
      901 agcatcaaat atctggaata gcgtgactag tagtataagc aatgctataa acgcagctaa
      961 aagtgccatt caaagtgttt ggaatagtat atcttcgtgg atcagcggaa tttggaacgg
     1021 tatcaaaaac actgctttga atctttggaa tggaattaca agcactatta gctctaaagt
     1081 aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga
     1141 catattcaat aatgttaaaa gtacaattgc taacatttgg gaaggtatca aaaagactgt
     1201 tagcgctcca attgattgga tcagagataa aatcagtagt atctttgata atttgaatat
     1261 ttctatacca catattccgt taccacattt taaattgagc ggagaattca atccattgaa
     1321 ggggaaaatc ccaacgttgg gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc
     1381 gaatattatc ggtgtcgg
//
LOCUS       DAJSVF010000215         1370 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSVF010000215 DAJSVF010000000
VERSION     DAJSVF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.226"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ON550_003004"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ON550_003004"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="ON550_003005"
     tRNA            203..276
                     /locus_tag="ON550_003005"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="ON550_003006"
     tRNA            316..405
                     /locus_tag="ON550_003006"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="ON550_003007"
     tRNA            420..491
                     /locus_tag="ON550_003007"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="ON550_003008"
     tRNA            507..579
                     /locus_tag="ON550_003008"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="ON550_003009"
     tRNA            589..661
                     /locus_tag="ON550_003009"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="ON550_003010"
     tRNA            697..772
                     /locus_tag="ON550_003010"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="ON550_003011"
     tRNA            774..854
                     /locus_tag="ON550_003011"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="ON550_003012"
     tRNA            861..934
                     /locus_tag="ON550_003012"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="ON550_003013"
     tRNA            937..1009
                     /locus_tag="ON550_003013"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="ON550_003014"
     tRNA            1021..1092
                     /locus_tag="ON550_003014"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="ON550_003015"
     tRNA            1138..1208
                     /locus_tag="ON550_003015"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="ON550_003016"
     tRNA            1229..1313
                     /locus_tag="ON550_003016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
BASE COUNT          331 a          305 c          354 g          380 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgaggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat
//
LOCUS       DAJSVF010000216         1370 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSVF010000216 DAJSVF010000000
VERSION     DAJSVF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.227"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            35..1336
                     /locus_tag="ON550_003017"
     CDS             35..1336
                     /locus_tag="ON550_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3956012.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          446 a          275 c          253 g          396 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAJSVF010000217         1368 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSVF010000217 DAJSVF010000000
VERSION     DAJSVF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.228"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            82..>1368
                     /locus_tag="ON550_003018"
     CDS             82..>1368
                     /locus_tag="ON550_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR3956013.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAJSVF010000218         1355 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSVF010000218 DAJSVF010000000
VERSION     DAJSVF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.229"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            240..794
                     /locus_tag="ON550_003019"
     CDS             240..794
                     /locus_tag="ON550_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3956014.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1048..>1355
                     /locus_tag="ON550_003020"
     CDS             1048..>1355
                     /locus_tag="ON550_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956015.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          549 a          144 c          237 g          425 t
ORIGIN      
        1 acttctacgg ataactgccc gtctaaaatt taaataagag cttagaatct tcatataagt
       61 aaggttaatt tttttgtaca aaaaagtcgg aatattctga gttactgaaa tgatataaaa
      121 tcataaacta aaccccttga ttttcttcac ttttaacaga gtatatatat aagttcatga
      181 ttatggtaaa atatatatat aatcatgaac tcagaatatt tttaagaagg gaaacaatag
      241 tgagctataa tgtacaaccg ttaaggacaa atgaagagat acaagatttt cagttttggc
      301 taagaagaac aagtaatcca gaaagagata gttttttatt tttgtttgga atcaataatg
      361 ggttacgtat gtcagatatt ataggattaa aagttggaga catacgaggt aaaagtaagc
      421 ctattattgt tgaacataaa acaggtaagc gaaaaccgat ttttatagat aatttgagag
      481 aagagatttt attatatact gaaggtaaag aagaaaatga ttggttattt ccaagtcggc
      541 aacaaggtcg acatataaca agggatcgtg tctatcagat atatgcagat atagcagaaa
      601 aattaggaag agatgatata ggaacacata cattaagaaa aacttttggg tatcactact
      661 acaagaaaac tagagacatt gctaccttaa tgtttatttt taatcatagc tcgcaagcaa
      721 ttacaaaaag gtacattggt ataactgaag atgaaatagg ggcatcgtta cgtggtttta
      781 aattgggagg ctaattgaga tattaggata tattttttgt gtataatgaa aacttagaag
      841 gtgagattaa tgaagataac tgaattttcg gaagtattta aaacgtttag taacatttac
      901 aatagaatac cagagataga acaaaatgtt gtagttgata atgattatta tatagagaat
      961 gattttaata aaccaagaaa atatgacaac ctatacaatt ttatacacaa tagaacaaca
     1021 ataaataaca acagtaacac tcgagttttg attaattcag catattttca tattaaatat
     1081 ttaaagaaat atgagtatga agaagttgta ttagattatg ggctaaatag acagtcattt
     1141 ttaatttatg caataaataa cggaaatttg ccaagcaata aaaagaacat aattattgaa
     1201 acatatgcct atctggaggg gggatctaaa aaactaatta agtcatgtaa tatagagtat
     1261 caattatcag taggagaaat taaatgtgtt ttcgaagaag atttagaact tgacttaaaa
     1321 aaaatttttg ccagcattca tgattatata ggttt
//
LOCUS       DAJSVF010000219         1320 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSVF010000219 DAJSVF010000000
VERSION     DAJSVF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.230"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            197..>1320
                     /locus_tag="ON550_003021"
     CDS             197..>1320
                     /locus_tag="ON550_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3956016.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGL"
BASE COUNT          506 a          210 c          280 g          324 t
ORIGIN      
        1 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
       61 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
      121 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
      181 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaacat tcatagctaa
      241 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
      301 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
      361 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
      421 tagccaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
      481 acttgagaga gaactgatta accaacaaac agcgcaacaa cgtttgtcaa acgaaattga
      541 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
      601 gcaacagttg gatagtgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
      661 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaatccgaga aattagcgaa
      721 agcccaagaa tatgtttctc aacaatctga aaatgcagag aaaacgatag atatcctgag
      781 acgacagtta gaagctacac agtctgagtt tggcgctaca tccacagaag caatgcagat
      841 ggaggcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
      901 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atctaatgga
      961 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
     1021 ggactctgct aatagtgtag gtagttccca gagtaaaata caagctaatt ttggtttgac
     1081 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
     1141 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggcgattt
     1201 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
     1261 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
//
LOCUS       DAJSVF010000220         1320 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSVF010000220 DAJSVF010000000
VERSION     DAJSVF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.231"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            197..>1320
                     /locus_tag="ON550_003022"
     CDS             197..>1320
                     /locus_tag="ON550_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3956017.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGL"
BASE COUNT          507 a          208 c          280 g          325 t
ORIGIN      
        1 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
       61 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
      121 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
      181 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaactt tcatagctaa
      241 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
      301 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
      361 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
      421 tagccaacgt ttagccaatg cgaaaaaata ttatggagaa aattccaccg aagttcagaa
      481 acttgaaaga gaactgatta atcaacaaac agcgcaacaa cgtttgtcaa acgaaattga
      541 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
      601 gcaacagttg gatagcgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
      661 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaagccgaga aattagcgaa
      721 agcccaagaa tatgtttctc aacaatctga aaatgcggag aaaacgatag atatcctaag
      781 acgacagtta gaagctacac agtctgaatt tggcgctaca tccacagaag caatgcagat
      841 ggaagcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
      901 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atttaatgga
      961 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
     1021 ggactctgct aacagtgtag gtagttccca gagtaagata caagctaatt ttggtttgtc
     1081 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
     1141 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggagattt
     1201 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
     1261 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
//
LOCUS       DAJSVF010000221         1316 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSVF010000221 DAJSVF010000000
VERSION     DAJSVF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.232"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1316
                     /locus_tag="ON550_003023"
     CDS             <1..>1316
                     /locus_tag="ON550_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR3956018.1"
                     /translation="KVLNDSKATQKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALY
                     NQVKSTAKGDFTEGSWNNFQTALSNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKP
                     KPSEYAVTVRHMNRQTGTLLKEEHVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQT
                     QTINTNTTFTFYYDEVFLVEVKARPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAP
                     MFTNFVLDPRESSSNTVTLNNVTSNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTV
                     YEGDSFSTSWKNMTDQNYFLCRNDDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSD
                     LNNYVRQKELAWLNSYRQQNGVAPMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQD
                     LLESLGCYGAKGENINSFYLYVDEILSDTGAVNAMVMWKDSAAHNA"
BASE COUNT          489 a          258 c          223 g          346 t
ORIGIN      
        1 aaagtgttaa atgactcaaa agcgactcaa aaacaagtgg atagtgccaa aaacacatta
       61 gattctgctt acaagggatt aaagaaaaaa ccagctccta ctgtgaacaa aacggaatta
      121 caagccttgt acaaccaagt aaaatcaact gctaaaggag attttacaga aggatcttgg
      181 aataattttc agactgcttt atccaatgct aaaaaagtac tagacgattc taaagcttct
      241 caaacccaag taaataatgc taaaaatagt cttgattctg catttaaagg gttacaacac
      301 aaacctaaac cttcagaata tgcggtaact gtccgtcata tgaatcgaca aactggaacg
      361 ctgctaaaag aagaacatgt ctcagtaaaa gctggtacaa gttacactgc aaaagctaga
      421 accgacttga ctaactttga agcaccacag tatcaagtaa atgggaatga aacacaaaca
      481 caaacgatta acacaaatac cacatttact ttttactatg atgaagtctt tctagttgaa
      541 gtgaaagctc gtccaaataa agtctcggat actgatccaa atatccaaga actctataat
      601 tacagtaaga gttatcccgt cgtttatgga caaagcatca caattgaagc acctatgttc
      661 actaattttg ttcttgatcc tagagaaagc tctagcaaca ctgtgacttt aaacaacgtt
      721 acaagtaatc aaggaatcaa ttttgcctat actcatcaat acaacgtcac tgtgaaccat
      781 ataaacgttg acacaaacgc tgttttatct actgaaacac aaacggttta cgagggagat
      841 agtttctcta cttcttggaa aaatatgacc gatcaaaact acttcttgtg tagaaatgac
      901 gattcatctg ttaccgttga taaaaatgga caacgtgtaa tcaataatgt tgaccaaaat
      961 cgaacgatca ctttcaaata caaaaatatc tcactaagtg atttaaacaa ctatgtgcgt
     1021 caaaaagaac ttgcttggtt aaatagctac cgtcaacaaa atggcgttgc cccaatgcaa
     1081 ttcaatgata tcgttcaaca agctgcagat attcgtgcaa aagaattaca agtaagcttc
     1141 agtcattacc gacctggcgg cggtactttc caagatctct tagaaagctt aggctgctac
     1201 ggtgctaaag gagaaaatat caatagtttc tacctttacg ttgacgagat cctttcagat
     1261 acaggagcag taaatgctat ggttatgtgg aaagatagcg ctgctcataa cgcaaa
//
LOCUS       DAJSVF010000222         1265 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSVF010000222 DAJSVF010000000
VERSION     DAJSVF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.234"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            46..>1265
                     /locus_tag="ON550_003024"
     CDS             46..>1265
                     /locus_tag="ON550_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3956019.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNA"
BASE COUNT          392 a          251 c          247 g          375 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct
      361 gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag
      421 aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt
      481 gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta
      541 tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg
      601 gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa
      661 cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat
      721 atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc
      781 gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa
      841 accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag
      901 cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg
      961 cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct
     1021 tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa
     1081 agagatcagt cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc
     1141 tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac
     1201 tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc
     1261 aatgc
//
LOCUS       DAJSVF010000223         1236 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSVF010000223 DAJSVF010000000
VERSION     DAJSVF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.235"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            992..>1236
                     /locus_tag="ON550_003025"
     CDS             992..>1236
                     /locus_tag="ON550_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3956020.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMEN"
BASE COUNT          460 a          200 c          203 g          373 t
ORIGIN      
        1 tataaaatat cccaacgtct catctaaaga aatgttgatc gtcagcaagg agctaaaaaa
       61 tacgaatata tttcttttgt agtaaaatcg gactggtttg catatcattt tcttcgtaaa
      121 tatcaaaatc atgttaacta ctatcaatta ctaataaaaa ctaccacttt aagcgttaag
      181 aactgttaag tttagttaag tgaggatagt tcacaaaatc cattgttaaa cttcacgaaa
      241 gtggtaaaac tcaaaatgct ttatatagga ttacagcatc gctctttctt cgattgcgcg
      301 ttgggtaaaa aatattatga agtaaaaatt gacgatgaca cgttccttac ggctcatcag
      361 atgaaacagc tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct aaaaaagcga
      421 ttgccatatt cacgcctcgc tcgacgaact attaaaggtc aagatttacc tgaagatcat
      481 cctttttagg aaattgaagc tttttatgtt gcacaatgca tttataatac tacaatgatg
      541 ttttcaccag atattcttct agggtgcggt gtaatgaaac agatacactt gcaaaagaaa
      601 attcaccgcg aatttactaa aattatgaat aattatgtta atcttccgaa atttttttcc
      661 aaaattaagg gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa aataacttct
      721 ttttaaacaa ttatagaaaa ccgatactct gttaagttgg ttgacacggt gtcgattttt
      781 atgactttct tattctgaaa cttcactaac ggacagtacc taaaaagtct atagtatgga
      841 cgtttacgag tactatttta tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa
      901 ctacccccac ttagcttgga taggctacaa ttaactagac agaaaaatta aggtgtgtag
      961 actagaagaa aacataccag gaggaatttt tatgtctaag agaacacgaa gaactttttc
     1021 acaagaattc aagcaacaaa tcgtcaatct ttacttagct ggaaagccac gtgtagaaat
     1081 cattcgagaa tatgaactaa cggcttcagc atttgacaaa tgggtaaagc aatctaaaac
     1141 gagtggttca ttcaaagaaa aagataatct tacgcctgaa caaaaagaat tgttagaact
     1201 acgtaaaaga aaccagcaat tagaaatgga aaatga
//
LOCUS       DAJSVF010000224         1199 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSVF010000224 DAJSVF010000000
VERSION     DAJSVF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.236"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            153..695
                     /locus_tag="ON550_003026"
     CDS             153..695
                     /locus_tag="ON550_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956021.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            851..>1199
                     /locus_tag="ON550_003027"
     CDS             851..>1199
                     /locus_tag="ON550_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR3956022.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          489 a          136 c          228 g          346 t
ORIGIN      
        1 gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc tagtctacac
       61 accttaattt ttctgtctag ttaattgtag cctatccagt ttggaaaaat aaaaaattat
      121 atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct ataaaatgct
      181 attgtgtaaa aaataaagga ggaataattt atgaaaaaat agtaccaaga ggagcatgcc
      241 tattacttgc attatgtttg agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca
      301 ataaaataaa agaaggacaa tccataagtt ctgaagagga aggcatgtca attaatgcaa
      361 atggcaaaaa tgaaactttt aagccatcag attatacatt ggaggcaaag aaagaatatg
      421 tttatgaata tttagggctt aaatttaaac tctcagacaa atttagaaat tatatagctg
      481 ataaaaagat agcgatgtta gatgatcaaa gtcctataga taaagaacta aagtatgcta
      541 ttttgacatt tgagaaaatg acagaagaac aaaagaatgc agttatagaa aagatgggcg
      601 atggatataa aaattggcaa aatgagcttg aaagaattgg gactattggt atatttgaaa
      661 aaaatacatc agaagaaaaa aatctaaaat tataaaatgt gatactcata ctaaaatagg
      721 agtttcaagt gatggaaaat acgattgcta tttaagcaca aatagcggag ctgaaagtaa
      781 tcttcttgat gaattaaaaa gaactgagat tcaaattata gataaaaaag aacgtcctaa
      841 aaatggtttt gtgctatcag aaaaaactga tttagaaaat acagaagcat ttaacaaaga
      901 atcggttaaa gatttacgta aactaccaac aaaggacata aatggcaagg attttacaag
      961 taaagatttt gagaagtatg atcttactat ggtaaatgta tttgcaactt ggtgtacagc
     1021 ttgtgtaaag gaaattcctg atttggttga ggttcagaat gaaatgaaaa gcaagggtgt
     1081 aaatatcgtt ggagttgtta cagatcctgt tgatgataac ggtgaaaaca aggaagcaat
     1141 tgaaaagtca aagttaatac atgaaaagac aaaggcttca tatcctttct taatgccag
//
LOCUS       DAJSVF010000225         1195 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSVF010000225 DAJSVF010000000
VERSION     DAJSVF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.237"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1076
                     /locus_tag="ON550_003028"
     CDS             <1..1076
                     /locus_tag="ON550_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3956023.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAJSVF010000226         1170 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSVF010000226 DAJSVF010000000
VERSION     DAJSVF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.238"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..753
                     /locus_tag="ON550_003029"
     CDS             <1..753
                     /locus_tag="ON550_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3956024.1"
                     /translation="IKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEK
                     EQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYA
                     NKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMD
                     RIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEI
                     EKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            825..>1170
                     /locus_tag="ON550_003030"
     CDS             825..>1170
                     /locus_tag="ON550_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956025.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          516 a          133 c          192 g          329 t
ORIGIN      
        1 ataaaaatat ttgataaaat taataattta ttcaagaatg acaatgctga attaaggaag
       61 ggtcttttag agtttaaact tcaagaacta gaacttcatt actcttattt agtgaaaatt
      121 accgaagaaa aagaacaaca aaaagcaata aaagaacaga tgatagaaga ggaaaaagtt
      181 cgtagagaaa ttgatcgtga aaagaaacgt attgataaag aacaacgtca attcaattct
      241 gaaatttcca aattaatcgc atatatgcaa aaagctaatg cagatgtgga aaaagaatta
      301 tatgcaaata aaattcaaga actggaagaa aaattaaaag aattagaagt tatacgtgaa
      361 aatgtattac aaagagaatt aaatactaga gccggttatg tgtatgtaat tagtaatatt
      421 gggtcttttg gtgaagacat atataaaatt ggtatgacta gaagattaga acctatggat
      481 agaataaaag aattgagtag tgcatcggtt ccatttgaat ttgacgttca tgcgatgatt
      541 ttttcagagg acgctccgtc tttggaaact aaattgcata atcattttag aaaacaagaa
      601 gttaataaaa ttaatcaaag aaaagaattt ttcaaagttt cactagacga aatagagaaa
      661 gttgttttag aaaattataa tggaacagta acatttacaa aattagctaa agctgaacaa
      721 tatagacgat ctttagaatt gtcaaaagat taaatatata aaaagccttc gggctttttc
      781 ttttaacaat taataggaac gtatattcga aaggaataga aataatgtgg ttagaaaaaa
      841 taaatgacaa aaaatttaag tacaccgaac gttatacgga tcccttaact gaaaaaaaga
      901 gaaaagtgtc cgttacttta tctagcaatt cacgccaagc ttggaatcaa gctaacttac
      961 ttttgaatga aaaaatcgca gaaaaaataa aacgaaatga agaattacca ctaacttttg
     1021 gagaattaaa aactaaatgg gatgaaaaat acaagccaac tgtaaaagaa agttcctatc
     1081 gaacaactca ggtttatcta tcattaattt caaaatatat aaaagatgat gtacttgtaa
     1141 aaaacgttaa tagtaatctt atacaagata
//
LOCUS       DAJSVF010000227         1142 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSVF010000227 DAJSVF010000000
VERSION     DAJSVF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.239"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..705
                     /locus_tag="ON550_003031"
     CDS             <1..705
                     /locus_tag="ON550_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3956026.1"
                     /translation="DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKV
                     DVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPN
                     AEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSN
                     EKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILL
                     GTLGLLGYKNRKKENE"
     gene            839..>1142
                     /locus_tag="ON550_003032"
     CDS             839..>1142
                     /locus_tag="ON550_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3956027.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFS"
BASE COUNT          462 a          144 c          227 g          309 t
ORIGIN      
        1 gatgccgata aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca
       61 gacgcatctg agggaattaa gaacaaagaa gatctaccaa aaggaactac ttatacttgg
      121 aaagagaaag tagatgttag tacagctgga aacaaaaaag ggattgtagt agtaacatat
      181 ccagatggat ctaaagaaga agtagaggtt actatttctg tagaagataa aaaagcaccg
      241 aataaacctc aagttgatcc tattacagag ggtgaccaaa ttgttactgg taaaactgaa
      301 ccaaatgcag aggtgacagt tacattacca aacgggagtc aataccatgg gacagctgat
      361 aaaaatggta attttacagt taaagttcct aaattagagg caggaacaaa agtgatagta
      421 actgcaactg atgaatctgg taatactagt gaacctacta atgtagttgt ttcttcaaac
      481 gaaaaagaca gtgaaaaagc cgttagtaaa gataataaga ctgacaatca aggtagcaag
      541 caaaacaaaa atagaggcaa aagcagtcct caaaagcagt cctcaaaagc ttatcctaag
      601 acgggagaga ttgatagtaa tatctttact attagtggtg gtttaatcct gttaggaact
      661 ttagggttat tagggtataa aaatcgaaaa aaagagaatg aatagaaaat caatttctta
      721 aatcttctct gaaaagagga agaatctttt tcttcctctt tttgtttttt attttgggag
      781 ataggttgtt agttaaaaat gaagatactt ttcatagaag ggaaaagaat gtgattaagt
      841 gaaaaaaata gtaattattg gatcaaacca ttcggggcta gctgcagctc gatttctaaa
      901 cttatgtgat tcaaattatg aagttattct gatagataaa aaatcagaaa ttagttactt
      961 agaatgtggg acatcgttgt tcataaaaag agaagttagc aatttagaag atctttttta
     1021 tcaaaataga agagaactaa attctaagat aaaaaagttg tttttggaga cagcagttga
     1081 caaaattgat attcaaaata aaaagattat ttgtgtaaaa aaagatggca caagattttc
     1141 cc
//
LOCUS       DAJSVF010000228         1122 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSVF010000228 DAJSVF010000000
VERSION     DAJSVF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.240"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            361..>1122
                     /locus_tag="ON550_003033"
     CDS             361..>1122
                     /locus_tag="ON550_003033"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3956028.1"
                     /translation="MNTMCEVSIIIPSYKGSNEILIAVWGALSQTVTSKEIIVVDDNG
                     IGTEEQIKTKEKLQKFIKENKIKYFAHEKNLNGSAARNTGIKASNGKYIAFLDDDDYI
                     IPSKLEEQINVLEKTPKEVGMSVCEGYYVHKDGKGYLKKIKGNYVALDKYLLDKHYFN
                     TSSIVIKRECLIDMKGFDDSFIRHQDWEFCTRMLSRYKSIVVHKPLFIKYSFNRNNPS
                     NIEKRIENLDFFFEKMEDKLKDNLSLNIVNRIYKFK"
BASE COUNT          437 a          114 c          200 g          371 t
ORIGIN      
        1 caattccaaa tttagcgtat actttcacag aaactatatt gggtttgtct tatggggaga
       61 caaataatta ttatatttta ttaccatctg tattaggaga agctataatg ctctgtggta
      121 agtacttcgc atttacctac gcgtttatct atggtattgt ttcaacttgg tttttgagat
      181 tgcttaaaag aaataagtct ttagaatatt tactaattta ttatttatta gatttcttta
      241 ggcaatttcg tggtgggagc cagtatgtta taactgcatg ggaaacccaa ttggttcctt
      301 taataataat agtattcttt tataaattag tgataaaaaa tggagtagcg aaagagagga
      361 atgaatacaa tgtgtgaagt ttctataata attccaagct acaagggaag caacgaaata
      421 ctaattgctg tatggggagc gttgtcacaa acagtgacat cgaaggagat aattgtcgta
      481 gatgataatg gaattggaac agaagaacag ataaaaacaa aagaaaaatt acaaaaattt
      541 ataaaagaaa ataaaataaa gtattttgcg catgaaaaaa atttaaatgg ttcagctgca
      601 aggaatactg ggataaaagc tagtaatgga aagtatatag cttttttaga tgacgatgat
      661 tatattattc catctaaatt agaagagcaa attaatgtat tagaaaaaac accaaaagaa
      721 gttggtatgt cagtatgtga aggatattat gttcataaag atggcaaagg ttatttaaaa
      781 aaaataaagg gaaattatgt cgcattagat aaatacttat tagataaaca ttattttaat
      841 acgagttcta ttgttataaa aagagagtgt ttgattgaca tgaaaggctt tgatgattct
      901 tttatacggc atcaagattg ggaattttgt acgcgaatgc tatcaagata taaatctatt
      961 gtggttcata aacctttgtt tataaaatat agctttaatc gaaataatcc atctaatata
     1021 gagaaaagaa tagaaaactt agatttcttt ttcgaaaaaa tggaagataa attaaaagat
     1081 aatttatctc taaatatagt taatcgtata tataaattta ag
//
LOCUS       DAJSVF010000229         1121 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSVF010000229 DAJSVF010000000
VERSION     DAJSVF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.241"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            32..862
                     /locus_tag="ON550_003034"
     CDS             32..862
                     /locus_tag="ON550_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956029.1"
                     /translation="MSLLIYTHVLSTFMSVIVIGFLFIGSLFSKRKEKKEMSDLILNM
                     IKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTNLQERALNISDSLIGAVNNDLVTFT
                     MGTIGVVSLILPLFFLKKFTNKEKIIYFCAVTTWFLSTNLFPWEILQKTPIQIIQFPW
                     RILGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAATEANYSHKIQSYK
                     GRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWRMHLIKHIIPK
                     "
BASE COUNT          378 a          164 c          196 g          383 t
ORIGIN      
        1 aaagaactgt gtgattcctt tagctatcag tatgagtttg ctaatctata cccatgttct
       61 ctccactttt atgagtgtga ttgttattgg cttcttgttt atcggctctc ttttctctaa
      121 gcgaaaagag aaaaaagaaa tgtcggatct gatattaaat atgataaaag ctgttggttg
      181 gaccattctt ttaacggctt acgtttggta tccgatgtta caacaagtcc tacatcaaaa
      241 aattaatcgt ccttttcgca caaatcttca agaaagggca ttgaatattt ctgatagttt
      301 aatcggtgca gtaaataatg atttagttac gtttacaatg ggaacaattg gtgtggtttc
      361 attgattctt ccacttttct ttttaaaaaa atttaccaat aaagaaaaga ttatttattt
      421 ttgtgcagtg accacttggt ttttatcaac gaatcttttt ccatgggaaa ttttacaaaa
      481 aacacctatt caaatcattc aatttccgtg gagaatcttg ggttttcaag tgctttttgg
      541 ttcattaatc ttaaccatag tgtttttaaa gtggaaaaca agcaacaaaa aaagtatgtg
      601 gagccttgta ggaatagttc ttattatttt tactgtgact gcagcaacag aagcaaatta
      661 ttcgcataaa attcagtcgt ataaaggtcg tttagttatg accaaaaaag atgttacacg
      721 ttatacatcc tcaagaactg gaggcctata tgactatgca ccattagatg cattgaaata
      781 taaagatcat ttaaagaaac atgaagtaaa agttggtgat cgatggagaa tgcatctcat
      841 aaagcatata attccaaaat agtctataca gtagaagatg ccgatgagca aaatatcaca
      901 ttacctgtat tcaattattg gggaacagtt gtgaaagtaa atgggaaaaa gagcactata
      961 gaaaatgaca atggtttagt gtctattaaa ggtacgggaa aaagaactat agttgaggtt
     1021 tcatctacgt atccaactac gatgattcta tctttattaa ttagttcaat aacttttata
     1081 ttcttattta cacgttattt aataagatgc tgcggttaaa t
//
LOCUS       DAJSVF010000230         1116 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSVF010000230 DAJSVF010000000
VERSION     DAJSVF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.242"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..156
                     /locus_tag="ON550_003035"
     CDS             <1..156
                     /locus_tag="ON550_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR3956030.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>1116
                     /locus_tag="ON550_003036"
     CDS             175..>1116
                     /locus_tag="ON550_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3956031.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERI"
BASE COUNT          475 a           88 c          198 g          355 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatt
//
LOCUS       DAJSVF010000231         1109 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSVF010000231 DAJSVF010000000
VERSION     DAJSVF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.243"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..864
                     /locus_tag="ON550_003037"
     CDS             <1..864
                     /locus_tag="ON550_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3956032.1"
                     /translation="ESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYM
                     KFPNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGC
                     MSIITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNP
                     GGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNM
                     VYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWI
                     DPLPFLNSSKK"
     gene            880..>1109
                     /locus_tag="ON550_003038"
     CDS             880..>1109
                     /locus_tag="ON550_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956033.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEID"
BASE COUNT          354 a          185 c          266 g          304 t
ORIGIN      
        1 gaatcagcgt ttggtacatc ggcgtccgca agaaatgatc tcaactattt tgggattact
       61 tggtttgatg gttgtctgtt tcctaaagga actgcacgag gaattggtgg cattgaaggt
      121 ggctggtaca tgaagtttcc caactcaaaa gctgcatttt cctattacgg ctttatggta
      181 gccacacaat caaattttaa tgcttgtgta ggaaataaaa gccctggcgc aagcttattg
      241 atcttaggac gaggcggtta tgccgctgca ggaattactg aagatagtcc gtattataca
      301 ggttgtatgt caattatcac ttctaataaa ttaaccgaat atgatgaatt cgccatcaaa
      361 cattggggtg aaggtggcaa taacaatggc acaattactg gtgaatggac gaatccattc
      421 cctggcagca gtttagataa aagttccttc agcggtggtc aattatttgg aacaaatcca
      481 ggcggtgaat tccgtccgaa cggcttccat gatggattag attttggttc agttgatcat
      541 ccaggaagtg agattcatgc ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt
      601 atttcaggtt tgggcgcgtg tgtgatcgtg attaattacg atggcttgaa tatggtgtat
      661 caagaatttg caaatagtac aggaaattct cgagtgaaag taggcgatca agtgaaagtt
      721 ggtcaagtaa ttgggatacg agatacagca cacttgcatt taggatttac tcgaatggat
      781 tggcgtcaag cacaaggaca tgcattcatt gatgatggta catggatcga tccgttacca
      841 tttttgaata gcagtaaaaa atagatcagg agaaaaaata tggaaaataa aacgttaaaa
      901 atcgtcttaa cagctgcaat tagcgttagt ttaatcggca atattgcgtt gttttggcag
      961 ctgaaagagg aacaagcagc aaatacctat aaaattgaag aagtggctaa aaacgcgtct
     1021 gagaagctca aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc
     1081 atcaatgaga aaaaccaaga aatcgatca
//
LOCUS       DAJSVF010000232         1109 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSVF010000232 DAJSVF010000000
VERSION     DAJSVF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.244"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..864
                     /locus_tag="ON550_003039"
     CDS             <1..864
                     /locus_tag="ON550_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3956034.1"
                     /translation="ESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYM
                     KFPNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGC
                     MSIITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNP
                     GGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNM
                     VYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWI
                     DPLPFLNSSKK"
     gene            880..>1109
                     /locus_tag="ON550_003040"
     CDS             880..>1109
                     /locus_tag="ON550_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956035.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEID"
BASE COUNT          355 a          193 c          265 g          296 t
ORIGIN      
        1 gaatcagcgt ttggtacgtc ggcgtccgca agaaatgatc tcaactattt tgggattact
       61 tggtttgatg gttgtctgtt tcctaaagga actgcacgag gaattggtgg cattgaaggt
      121 ggctggtaca tgaagtttcc caactcaaaa gctgcattct cctattacgg ctttatggta
      181 gccacacaat caaattttaa tgcatgtgta ggaaataaaa gccctggcgc aagcttattg
      241 atcttaggac gaggcggtta tgccgctgca ggaattacgg aagatagtcc gtattataca
      301 ggatgtatgt ccattatcac ttctaataaa ttaaccgaat atgatgaatt cgccatcaaa
      361 cattggggtg aaggtggcaa taacaacggc acgatcactg gtgaatggac gaatccattc
      421 cctggcagca gtttagataa aagttccttc agcggtggtc aattatttgg aacaaatcca
      481 ggcggtgaat tccgtccgaa tggcttccat gatggattag actttggttc agttgatcat
      541 ccaggaagtg aaattcacgc ggtgcatgga ggaaaagtcg tgtacgttgg caatcctggt
      601 atttcaggtt taggtgcatg tgtgatcgtg attaattacg acggtttgaa tatggtatat
      661 caagaatttg cgaatagtac aggaaattct cgtgtgaagg ttggcgatca agtgaaagtt
      721 ggtcaagtaa tcgggatacg agatacagca cacttgcatt taggtttcac tcgaatggat
      781 tggcgtcaag cacaaggaca cgcattcaca gatgatggta cttggatcga tccattacca
      841 tttttgaata gcagcaaaaa atagatcagg agaaaaaata tggaaaataa aactttaaaa
      901 atcgtcttaa cagctgcaat tagcgttagt ttaatcggca atatcgcgtt gttttggcag
      961 ctgaaagaag aacaagagac aaataccttt aaaattgaag aagtggctaa aaacgcgtct
     1021 gagaagctca aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc
     1081 atcaatgaga aaaaccaaga aatcgatca
//
LOCUS       DAJSVF010000233         1107 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSVF010000233 DAJSVF010000000
VERSION     DAJSVF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.245"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            87..497
                     /gene="spx"
                     /locus_tag="ON550_003041"
     CDS             87..497
                     /gene="spx"
                     /locus_tag="ON550_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3956036.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            511..867
                     /locus_tag="ON550_003042"
     CDS             511..867
                     /locus_tag="ON550_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3956037.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          383 a          161 c          210 g          353 t
ORIGIN      
        1 atttttttta tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg
       61 gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt
      121 cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata
      181 tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag
      241 gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta
      301 acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac
      361 gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt
      421 catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata
      481 acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac
      541 tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt
      601 gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa
      661 gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt
      721 gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc
      781 ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg
      841 atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt
      901 tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac
      961 tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg
     1021 ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt
     1081 gttttaagta gcaaagcaat aaaatag
//
LOCUS       DAJSVF010000234         1094 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSVF010000234 DAJSVF010000000
VERSION     DAJSVF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.246"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..280
                     /locus_tag="ON550_003043"
     CDS             <1..280
                     /locus_tag="ON550_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3956038.1"
                     /translation="KRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLS
                     QGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            623..879
                     /locus_tag="ON550_003044"
                     /pseudo
     CDS             623..879
                     /locus_tag="ON550_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          379 a          164 c          198 g          353 t
ORIGIN      
        1 taaacgtaat cccttcgtat ctgaatatgt taaacgactt gcaaaaggta tttgtcaact
       61 ttgccaagaa aaagggccat ttataaaaga cggtgtacct tatttacatt gtcatcatat
      121 agaatatttg tcgcaaggtg gaaaagatgt gattgaaaac tgtatcgcgt tatgtcccaa
      181 ttgtcatgct agaattcatg aacttgagtt acctgacgat aaagaaaaat tgctgaagat
      241 cgttgaaaaa agagaaaata gcttttctaa aataccgtaa tttattttta taggacaaat
      301 aagctaatgt gagctatgcc ttctatataa gactaagttc tattataagt aacgaagtaa
      361 ttctcgttcc ttcctgtagt aatatttttt aaataaccta taggcttctt caaaagtaca
      421 tcctccagta aattctaagt actggaggat tctttttcta gtgttgcttt actcatgaaa
      481 aagtagttgg ctacttcatc aaaatttctg aactttttta atgtataata aagagctcga
      541 tcatttacta caaggatgct agctcctaca ttttcacagt tttttagcaa gtaaattttt
      601 ctaaaatcac atcaggaaat gcatgagatt ctttaagaaa ggcaattata gggctatgat
      661 tacggttcgt gtaagtgacg ccaaaaaaga atggctttct tatatggctg aattttatgg
      721 gattacttta tctgaacttc taaaaaacta ttcgatggaa caactagaag atgaatacaa
      781 tcgtcaaacg gctgaaattg cccataaaca ttggataaga agtgggaaaa caacagtatt
      841 catggaagaa tccttaatga atttggtggg ctggaatgac ctgccgttta aaattcacgc
      901 ccgaaattca gaagcagctc tgattggcaa atacgtgtat tggtagagat agaagataat
      961 caattcgttg ttaccgctat tcaagtaggg caccgaagaa atgtctatga ttaagagact
     1021 gtttattttt acaaaaaaat aataactgga atctagttaa aactcttgaa gtgagagatt
     1081 tgactagatt tttt
//
LOCUS       DAJSVF010000235         1087 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSVF010000235 DAJSVF010000000
VERSION     DAJSVF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.247"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..265
                     /locus_tag="ON550_003045"
     CDS             <1..265
                     /locus_tag="ON550_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956039.1"
                     /translation="LTKSESWQEDKEIVAEVQKLGKSMWTEKPKRKTPRKIAIWHGDR
                     ILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            265..621
                     /locus_tag="ON550_003046"
     CDS             265..621
                     /locus_tag="ON550_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3956040.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            608..826
                     /locus_tag="ON550_003047"
     CDS             608..826
                     /locus_tag="ON550_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956041.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            847..936
                     /locus_tag="ON550_003048"
                     /pseudo
     CDS             847..936
                     /locus_tag="ON550_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1011..>1087
                     /locus_tag="ON550_003049"
     CDS             1011..>1087
                     /locus_tag="ON550_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCR3956042.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNV"
BASE COUNT          421 a          147 c          256 g          263 t
ORIGIN      
        1 attaaccaag tcagaatctt ggcaggaaga caaagaaata gttgcagaag tccaaaagct
       61 cggtaaatca atgtggactg aaaagcctaa acggaaaacg ccgagaaaaa ttgcaatctg
      121 gcatggtgat cgaattctag taacaggtac agctgaacag ttatctgaaa ttactggatt
      181 aagcaaaaac attatctggg atagagctag gagcttatgg attgattcaa aaggacgaca
      241 gtttaggtat gtggaggaga gataatggat ctcattacac aatacagtga catcatcctc
      301 aagaaaatca tgatgaagat tcagaaagac aaaaaatcaa aagaacgagc tgaattagtt
      361 aagttagaaa tggctgaaac aggagcagga gtgcgaagta gcaggcattg gaaagcagca
      421 gcaaacattg aattttatta caacgaaatt caaaaagggt tcgatcagat gcgtgagctg
      481 gatcggcaaa caaattggag caagaaactt catcaagatc gtttcaaatt tgtagagaag
      541 tataaagaaa tattagaaga gtatttgagg aggacagcaa atgataaaaa aactcgttca
      601 attcagcatg gatttatatg atatcgaatc aggagcaaca ctatctgtgg aatcggacca
      661 tctaatcata aattttggtg gaaagcgcca gattattttg tgggtagttg atgatgtact
      721 gtttccagaa attgttcatg atttcgaaga atcaaaagcg gttgagtttg aaatagtgaa
      781 aaaagtaatg gaattgattg aaaaatacga ggaggacagc gaatgatacc gaagtttaga
      841 gcgtgggata gagaaaaaga cagaatgaag gcagtagctt tggttgaatt tgctgaggcg
      901 tataaatcag gagaactcat ttcacgtaca aaataaaaaa agtcggattc ctccgactat
      961 gaataatatt tccgacataa gtattatatc ataattgggg gaatcagagg atggtacttt
     1021 tcgatgtaaa gaaatatgag acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac
     1081 ataatgt
//
LOCUS       DAJSVF010000236         1074 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSVF010000236 DAJSVF010000000
VERSION     DAJSVF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.248"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(124..197)
                     /locus_tag="ON550_003050"
     tRNA            complement(124..197)
                     /locus_tag="ON550_003050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Ile,seq:cat)
     gene            complement(752..1066)
                     /locus_tag="ON550_003051"
     CDS             complement(752..1066)
                     /locus_tag="ON550_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956043.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          402 a          169 c          166 g          337 t
ORIGIN      
        1 tcattaatcc ataaaaggat acataaaaaa gccactcatt tgagtggcaa tgaagaaaag
       61 ctttagcttg tataatactc ttcaaaaaat tctaacacag aacgattcaa ttggctacat
      121 taatgtaccc tgtaggactc gaacctacga ccggacggtt atgagccgtc tgctctgacc
      181 aactgagcta agggtagtgg ttgttgccac ataaagccat aaacaatcaa ccagtagaat
      241 gcgtggcaac aaacctgtta tcgcatatct tggagtgtga ctatttatga gtgatagtga
      301 agatatgcgt ctccatatat gtatggttga gacagataaa catgtatgaa aaacgttgtt
      361 aaatcaacgt ttttttattt tggtcaagga gagaaaagta gagaaataca aaattttttg
      421 cattgatttt gcattcaaaa aaacattcaa acatttagaa atccccaata cgtattcagg
      481 aaaatctatt ttaaggttta caaaaagaac ttttgttcgt acactctttt tgaggtgaac
      541 ttttatgctg atggaaggaa aaacacaatt atggtttaag catgttcctt caaataagtt
      601 ttttaattat aaactctaga gaaattaatt catcctaaca taaaataatt gttcactcaa
      661 aagtttttct agaacaatta aaacagcagg cttcacattc gctatacatt tgtaagaagt
      721 aataattcct gtactaaaag gcataattgc tttaattatt ttttgacttc tcttcaaaca
      781 caagaagtag ttgtgtaata attgcagaaa taaggattcc aataatagat ataaaatttg
      841 acatggaaac gtctataaca ttaaaagaat aaagtgttga agaaagtaaa ctgattgaaa
      901 taacccctcc gtaaaaaata gtctgtgaat atacccactt tttttcagaa gacgtacttc
      961 gtttggttct aaaacctatc cctgcatttc tactcttatc aaaaaaccaa atatttagaa
     1021 gtaataatat tgctattatt aagataaaat tactgtaaaa atccaaaagc tatc
//
LOCUS       DAJSVF010000237         1050 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSVF010000237 DAJSVF010000000
VERSION     DAJSVF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.249"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1050
                     /locus_tag="ON550_003052"
     CDS             <1..>1050
                     /locus_tag="ON550_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3956044.1"
                     /translation="GSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNL
                     YIKRNMQVNNIFRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQ
                     ENIKEDLGLIATVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDF
                     WGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPE
                     PTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILE
                     TRPGFSHQMKIPVTDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLF
                     SDPQKQIDEENLDKI"
BASE COUNT          372 a          188 c          221 g          269 t
ORIGIN      
        1 tggttcagga ttgatattag cttcttctcc tgaagcaaaa aagcggtatg gtattacaaa
       61 tgtgagtaga ccacgtgact taccacaacc atttcctaaa acactacacg ttgttccacc
      121 acgtatgaac ctatatatca agcgaaatat gcaggtaaat aatattttca gaagatatgt
      181 ggctgatgaa gatctactga tttactcgat cgatgaatca atcttaaaag tgacccgatc
      241 actgaatctt tttacgactg aaggaacacg aagtcaacgg agaaagaagc tggctcaaat
      301 gatccaggag aatattaaag aagatctagg attgattgct acagtaggtg tcggagataa
      361 tccccttttg gctaaactag ccttagacaa cgaagcaaag cataatgaag gatttgttgc
      421 ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat attcctgaaa tgacggactt
      481 ttggggaatt ggctctcgaa tgaaaaaaag actcaatcag atggggatta tgagcattag
      541 agatcttgct aactggaatc cttatacgat taagaataga ctaggtgtga ttggcttaca
      601 gctgtacttc catgctaacg ggatcgatcg aacagatata gcgatcccac cagaaccaac
      661 caaagagaaa tcctatggta attcacaagt tttgccacga gactatacta ggagaaatga
      721 aatcgaacta gtagtaaaag aaatggcgga acaagtagcc attcgtatca gacagcataa
      781 ttgtaaaaca ggatgtgtcc atttacacat tggcacgtcg atcttagaaa cgagaccagg
      841 attctcgcac caaatgaaaa tacctgtcac agacaataca aaggaattgc agaattattg
      901 tcttttctta tttgataagt attatgaagg acaagaagta aggcacgtcg gaataaccta
      961 tagcaagctt gtgtacaccg attcgcttca gctggattta tttagtgatc cgcaaaaaca
     1021 aatcgatgaa gaaaacctgg ataagattat
//
LOCUS       DAJSVF010000238         1035 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSVF010000238 DAJSVF010000000
VERSION     DAJSVF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.250"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>1035)
                     /locus_tag="ON550_003053"
     rRNA            complement(<1..>1035)
                     /locus_tag="ON550_003053"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAJSVF010000239         1006 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSVF010000239 DAJSVF010000000
VERSION     DAJSVF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.251"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            108..971
                     /locus_tag="ON550_003054"
     CDS             108..971
                     /locus_tag="ON550_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3956045.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
BASE COUNT          367 a          167 c          195 g          277 t
ORIGIN      
        1 ttttttcacc tttccaaacc aaggctcagc tattcaagta gataacatta tgttatgata
       61 gcaaagaaat gtattaagaa aacctttcac tatagaggga gaaaaaaatg aacgatttat
      121 tagcaactat atttacaggt attattattg atgaaaatga aaaaaactac tttgtccaaa
      181 aaaacggaca aacctttggc ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag
      241 ttgaaggatt cggttaccaa aatcaaaaac atgaaaatcg gatcactaca caaattccta
      301 aaagccgtat cggtcattac gcttttggaa tcgttactgc tacacgaaaa gatttaggtg
      361 cgtttgtcga tatcggtctt ccagataaag actttgtcgt atctcttgat gaattgccga
      421 caatgacaga gctatggcct aaaaaagggg atcgcttgat gatttccctt agagttgatg
      481 caaaagaccg tatttggggc agtttagcag atgaaaaaat attcaaatct ttaagcaaat
      541 atggaacaga agaaatgaaa aacaaagatg tgataggaac agcataccgc ttgaaattaa
      601 caggtacgta cgttttaacg gaagatttct atcttgggtt tatccatcct tctgaacgat
      661 ataatgaacc tcgtctaggc gagcagataa aaggtcgagt gattggtgtc cgaccagacg
      721 gtgtactgaa tctttcattg aagccaagag catatgaagc tatatcagat gatgcacaaa
      781 tgattttaac cattttagaa cgttctgcag atcagcaaat caactttaca gataaatcgg
      841 atccagaaga aatcatgaaa gcttttggta tcagcaaagg agcatttaag cgcgcattag
      901 gtaatctttt gaaacaagga ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa
      961 aatctaattg agaatccctt gcatgttttt cttaactagc ttataa
//
LOCUS       DAJSVF010000240          982 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSVF010000240 DAJSVF010000000
VERSION     DAJSVF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.252"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            349..982
                     /locus_tag="ON550_003055"
                     /pseudo
     CDS             349..982
                     /locus_tag="ON550_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          333 a          181 c          179 g          289 t
ORIGIN      
        1 atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat tatctaaatc
       61 tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta ctattcatgg
      121 tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt aaagatgtgc
      181 aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt catattacag
      241 acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat aatttatggg
      301 actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat gattcgtaca
      361 ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc aaaagtattg
      421 atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt tatttgtgcg
      481 gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc tcgattaaca
      541 cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac agatatgaac
      601 gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt cattgattgt
      661 tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca agaaatgaaa
      721 agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa ctggcgcttt
      781 cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg cagttttcgt
      841 gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct ggcattttca
      901 acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt tagagacaag
      961 gaatcagact tattttttac ct
//
LOCUS       DAJSVF010000241          981 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSVF010000241 DAJSVF010000000
VERSION     DAJSVF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.253"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(25..255)
                     /locus_tag="ON550_003056"
     CDS             complement(25..255)
                     /locus_tag="ON550_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956046.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(505..705)
                     /locus_tag="ON550_003057"
     CDS             complement(505..705)
                     /locus_tag="ON550_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3956047.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          341 a          156 c          151 g          333 t
ORIGIN      
        1 gcgtctcatt aaaaagcata accattactc cgatctattt ttaaacttag tctctacctc
       61 taatatatat cgagttttta ttttgccttc agagaatact gtttcttttt ttgcagttac
      121 aggttgttta ttttcggaaa aagctaatat agctaaaatt gaaacatcca tctggaattt
      181 gtctttttcg ttgctttgct cataaaaatc tgcatattca tcactaatat tttttctaat
      241 aaattcttcc atcataaaga tcacctcgaa aagagtatac gaacaaacgt tctttttgta
      301 aaggtggaat ttattatact aaataaatag gtgaacaagt acttgtgcca aattatgtgc
      361 caaaaaaaat cgaatttaat aaaacacaaa caaaaagaaa tcctattatg ataacatttc
      421 ttataataac aaacacaaca gaagacgtgt aatagttagt caggaacgta caaataaccc
      481 ctgtatcctt tgcggtacag ggggttattt tgtataagta gacattgtag tttctaaact
      541 gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga
      601 tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga gaaaataggg aaaaattgat
      661 tactaaagaa aaacttacta ggtcgacggt gtagttgatt ttcatacttc aatagattcg
      721 aattaaacga tttcgattgg cttataccag tcctatccaa cgaaagatat atcgttctta
      781 actctagcgg tataacttga agctttacgt aaagccaatg ggaatgaact ggagatgact
      841 ttttattatt tttagtaata gctgatgggt taatacttac aactttcctt ttcaaagtac
      901 catataaaaa aacggtgggg ttgtttagcg aaataaagga tctaagccaa tagcttagat
      961 cctttattct atgctttata t
//
LOCUS       DAJSVF010000242          970 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSVF010000242 DAJSVF010000000
VERSION     DAJSVF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.254"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            65..>970
                     /locus_tag="ON550_003058"
     CDS             65..>970
                     /locus_tag="ON550_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3956048.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          346 a          172 c          168 g          284 t
ORIGIN      
        1 gttggtaaat caacaaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact
       61 acatatgcgt tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga
      121 aattatgaga actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg
      181 aagaaatact gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg
      241 cataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt
      301 tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta
      361 cactatcaag attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg
      421 aattatagat agttttccta gtccattatg taaaccagtt agaaatagac aagcaaaact
      481 attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct
      541 taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc
      601 aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat
      661 cctaggggac aaaggatata ttagccataa aatccatgaa aagcttgccc ttaagggcat
      721 tactatatcc gttccgccac gtaaaaacat ggataaatca gaaaaactag accacagctt
      781 acttggaaaa caaagaaaaa cagttgagac tgttttttct tccttagaaa aattaggttg
      841 tcaaaatttc aactcacgtt ctgttaaagg gctagagagt agatttgaaa gcatattact
      901 ggcttacagt gttctattaa gtcgagcaca acgacgtttt gaaggaactt tgagatattc
      961 tttaggatat
//
LOCUS       DAJSVF010000243          952 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSVF010000243 DAJSVF010000000
VERSION     DAJSVF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.255"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            75..>952
                     /locus_tag="ON550_003059"
     CDS             75..>952
                     /locus_tag="ON550_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3956049.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWE"
BASE COUNT          296 a          207 c          189 g          260 t
ORIGIN      
        1 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
       61 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
      121 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
      181 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
      241 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
      301 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
      361 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
      421 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
      481 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
      541 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
      601 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
      661 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
      721 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
      781 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
      841 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
      901 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa tt
//
LOCUS       DAJSVF010000244          937 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSVF010000244 DAJSVF010000000
VERSION     DAJSVF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.256"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..881
                     /locus_tag="ON550_003060"
     CDS             <1..881
                     /locus_tag="ON550_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3956050.1"
                     /translation="DSLETTIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLV
                     AEDVLAFKERTLEANYSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIA
                     PTESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNI
                     AHKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTF
                     YNFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGR
                     IHKGFKEIQDTLESMI"
BASE COUNT          325 a          155 c          173 g          284 t
ORIGIN      
        1 atgattcatt agaaacgacc attatccaat tatttcaaaa aggcattact atggctgaaa
       61 tctccaaatt aattgaaaaa atgtatggtc accactatac gccacaaaca atctccaaca
      121 tgagtaaatt ggtggctgaa gatgttttag cttttaaaga aagaacgtta gaagccaatt
      181 attccgttat atttatggat gcgacgcata tccctgtgaa gcgacagact gtttcgaagg
      241 aagcagtgta tattacgatt ggtattcgtt tggatggaac caaagaagtt cttgggttta
      301 ctattgcccc aactgagtct gcttatattt ggaaagaagt tcttcaagat cttagaaaac
      361 gtgggttaga agaagtttta ttagtagtga cagacggatt aagctgtatt gaagaaagta
      421 tccatagtgt gtatccgaat gcccaatttc aacaatgttg tgtgcatgta tctagaaata
      481 tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc aaattggttt
      541 accaagcgaa ttcaaaagaa gaggcattgg atcacatcga ctttatgact aggaaatgga
      601 aaaagcagta tccaagagtc gtcaatttac tcttgaatcc tgccctatta accttttata
      661 atttccctca cgccatcaga cgaacaattt attcgacgaa cctgattgaa ggctttaata
      721 agcagctaaa acgatatact cgaagaaaag aacaattccc taatgaagaa tctctagaga
      781 gattcttcat ttctcaattt aatcaatata accaaaaatt tttaggtaga attcacaagg
      841 gatttaaaga aattcaggat acattagagt cgatgattta actgtaatta acagaatgga
      901 ttttccattt acacataatt cttgacgcta cctttaa
//
LOCUS       DAJSVF010000245          926 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSVF010000245 DAJSVF010000000
VERSION     DAJSVF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.257"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            6..>926
                     /gene="bsh"
                     /locus_tag="ON550_003061"
     CDS             6..>926
                     /gene="bsh"
                     /locus_tag="ON550_003061"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3956051.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSH"
BASE COUNT          319 a          123 c          168 g          316 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcat
//
LOCUS       DAJSVF010000246          902 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSVF010000246 DAJSVF010000000
VERSION     DAJSVF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.258"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            167..868
                     /locus_tag="ON550_003062"
     CDS             167..868
                     /locus_tag="ON550_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3956052.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          327 a          158 c          147 g          270 t
ORIGIN      
        1 tcgcttcaaa acaaattatt caataccatt gtatattttt tacataataa caaattatat
       61 ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca aattctttaa
      121 aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc gtgttaataa
      181 aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt tctcggatga
      241 agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag gacagatcct
      301 tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac tcgttcgttt
      361 agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata caaataacct
      421 ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc aagcgatgac
      481 tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc
      541 cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac atgaattgcg
      601 tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt tattcatttt
      661 gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt atccgatcac
      721 cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac aagtaatcaa
      781 acaactacgt gattccgggc tgttagatta tgacaaaaag cgttttactt tctgtaagac
      841 agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga ccctgataaa
      901 ta
//
LOCUS       DAJSVF010000247          856 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSVF010000247 DAJSVF010000000
VERSION     DAJSVF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.259"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(101..301)
                     /locus_tag="ON550_003063"
     CDS             complement(101..301)
                     /locus_tag="ON550_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3956053.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            697..846
                     /locus_tag="ON550_003064"
     CDS             697..846
                     /locus_tag="ON550_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956054.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          281 a          146 c          132 g          297 t
ORIGIN      
        1 gttttataca ctactattca gcccattcaa cgaccaatga attttttcgt acaaaaaaac
       61 cgtcttttct ttcgaaaaga cggttaaaag tcaattttta ttaagcttta acaatgtttg
      121 ttgcttgagg gccacgttga ccatcttcaa catcgtaaga cactgcttgg ccttcttcta
      181 aagttttgaa gccgtcacct tggatagctg aaaaatgtgc aaatacatca tttccatctt
      241 caccagtgat aaagccaaaa cctttgtctg cgttgaacca ttttactgta ccgttattca
      301 taaataaatt cctcctgata cgtatcaatg tacgtttttt cgttgcaata aatatttgat
      361 aggcaaaagg agttttatct atgtagatat atcgctcaaa ttacgtttca aaatagatta
      421 ctcatttaat atatcatgct ttttctaata acacaaggat aatgcaatga aatataaaat
      481 tcattgtctt ctattgtctt cgaatcgaag aaaaagttga ataaccgcga ttgattcact
      541 ttttgttttc ttgtttattt gcttttatct gcaacttttg actttaaaat ttcttaacta
      601 tgaaaacctt tcaattggtg gtatacttat aaatggaatt tcgttatcaa cgcgttcgtt
      661 gcctttcagc aagtatttaa agaaaggaga atggctatga cattctataa caaagatata
      721 caatatcgag taaccttgga tacagatttg aatctatttg tcgtttttga caaagaaaat
      781 accaatcatg tagcgacagg tgttacgatc gaacaagcag tgcaagaatt aaaaaaatct
      841 gcataaggag tggaaa
//
LOCUS       DAJSVF010000248          797 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSVF010000248 DAJSVF010000000
VERSION     DAJSVF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.261"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ON550_003065"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ON550_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3956055.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ON550_003066"
     CDS             complement(481..675)
                     /locus_tag="ON550_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956056.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          241 a          168 c          136 g          252 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacttt ataaaggtcg tccaaaactc
      781 tatagtgcag atacaaa
//
LOCUS       DAJSVF010000249          794 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJSVF010000249 DAJSVF010000000
VERSION     DAJSVF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.262"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..242
                     /locus_tag="ON550_003067"
     CDS             <1..242
                     /locus_tag="ON550_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3956057.1"
                     /translation="NGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPEV
                     KKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            253..>794
                     /locus_tag="ON550_003068"
     CDS             253..>794
                     /locus_tag="ON550_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3956058.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSL"
BASE COUNT          278 a          147 c          173 g          196 t
ORIGIN      
        1 cgaatggtac gtacgatttt gatcgttttt atcaaggaaa aggggatatt aatgctcaga
       61 ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaagcat gaacatgaat
      121 ttgtacctga agtgaaaaag gattggaacg atcaattgaa gcatgagatc cagcaacaag
      181 aaattagaaa gcaaaaacgt gcgatgttgt ttcaacaagg cagacaacaa gaaagggagt
      241 gagatcgtca cgatgaaaac tgtcatttta gcagaaaaac cgtcacaagc gaaggcttat
      301 gccgatagct tttctaaagc cacccgaaaa gacgggtatt ttgagatcca agatcgattg
      361 tttcctggtg aaactgtgat tacgtatggt tttggtcatt tagtcgagtt agattcgccc
      421 gatatgtacg atgaaaattg gaaacaatgg tcattagaac atttacctat tttccctaca
      481 cattatcact atcatgttcc taaagataag aaaaagcaat tcaacgtggt gaaacagcaa
      541 cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgcgaagg ggaactgatc
      601 gcttggacga tcattcaaca agcaggcgcc gatcatggaa agatatttaa aagactttgg
      661 atcaactcct tagaaaaaga agccatttat cagggattcc agcagttacg agatgcagaa
      721 gagacctacc caaaatttga agaagcacaa gcacgccaga tcgccgattg gttgatcggc
      781 atgaacggta gcct
//
LOCUS       DAJSVF010000250          794 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSVF010000250 DAJSVF010000000
VERSION     DAJSVF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.263"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..242
                     /locus_tag="ON550_003069"
     CDS             <1..242
                     /locus_tag="ON550_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3956059.1"
                     /translation="DGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPEV
                     KKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            253..>794
                     /locus_tag="ON550_003070"
     CDS             253..>794
                     /locus_tag="ON550_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3956060.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSP"
BASE COUNT          280 a          141 c          172 g          201 t
ORIGIN      
        1 tggatggtac gtacgatttt gatcgttttt atcaagaaaa aggggatatt aatgctcaga
       61 ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaatcat gaacatgaat
      121 ttgtacctga agtaaaaaag gattggaatg atcaattgaa gcatgaaatc taccaacaag
      181 agatcagaaa gcaaaaacgt gccatgttgt ttcaacaagg tagacagcaa gaaagggagt
      241 gaaatcgtca tgatgaaaac cgttatttta gcagaaaaac cgtcacaagc gaaggcttat
      301 gccgatagct tttctaaagc tacccgaaaa gatgggtatt ttgagatcca agatcgattg
      361 ttttcaggag aaactgtgat cacgtatggt tttggtcatt tagtcgagtt agattcgccc
      421 gatatgtacg atgaaaattg gaaacaatgg tcattagagc atttgcctat tttccctaca
      481 cattatcact atcatgttcc taaagataag aaaaagcaat tcaaggtggt gaaacagcaa
      541 cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgtgaagg ggaactgata
      601 gcttggacga tcattcaaca agcaggcgcc gatcatggaa agatatttaa aagactttgg
      661 atcaactcct tagaaaaaga agccatttat cagggattcc agcagttacg agatgcagaa
      721 gagacctacc caaaatttga agaagcacaa gcacgccaga tcgccgattg gttgatcggc
      781 atgaacggta gccc
//
LOCUS       DAJSVF010000251          791 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJSVF010000251 DAJSVF010000000
VERSION     DAJSVF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.265"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..782
                     /locus_tag="ON550_003071"
     CDS             <1..782
                     /locus_tag="ON550_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3956061.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DAJSVF010000252          789 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSVF010000252 DAJSVF010000000
VERSION     DAJSVF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.266"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..789
                     /locus_tag="ON550_003072"
     rRNA            <1..789
                     /locus_tag="ON550_003072"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          196 a          172 c          251 g          170 t
ORIGIN      
        1 catgtacagg ataggtagga gccgtagaaa tcggaacgct agtttcgatg gaggcgctgg
       61 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
      121 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      181 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      241 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      301 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      361 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      421 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      481 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      541 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      601 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      661 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      721 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      781 gaggactta
//
LOCUS       DAJSVF010000253          783 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJSVF010000253 DAJSVF010000000
VERSION     DAJSVF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.267"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..317)
                     /locus_tag="ON550_003073"
     CDS             complement(<1..317)
                     /locus_tag="ON550_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956062.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTT"
BASE COUNT          228 a          185 c           95 g          275 t
ORIGIN      
        1 tttgttgtaa cctcctcata atcagttagt actataaagg tatctttgtt tttatccaaa
       61 aaatctaata aatcttttgt agaagatatc tcttcatcaa cttcaccata ataaatacct
      121 tcgataattg ttgctatatc tgaaaaacta atttttactg gacatttatc gtctaccgca
      181 ttcaataccg acaaaattgc ttctttttct gcaatctctc gtttgaattt ctttggagct
      241 aatcgcatga cactcaagtc attttcaagc ttttcaatcc atgcagtttt actcattttc
      301 gcaaattttt gaatcattcc tctaccacct ttgctatcta ctaaattagt tttacttcat
      361 aacgaatatc tttatcgttt ttcgttcact caaaaatata taaaataaat cgactcgttt
      421 atggcgtccg aaacgagaaa ccctagtaga cagcggtaaa tgctcgattg atccaatgat
      481 caacgtcctc gcaagtatct acatgtactg cagcacacga cacaatttct tgtgcgtcct
      541 ccccattacg agaagaccta ctccttattc catgagtttg cgtcaaagcc tagatccctt
      601 agtagctgct catgattaat ctaggcgcta ttctaacttg tctaacttct aaattccctc
      661 ttgtttactc tgtgcatatt atttacacaa attcatttca ccactatcca aacaagggca
      721 tttactcgat tgaccaattt tttacgttac caatcgtaaa aacctacccc gtcactcccc
      781 gaa
//
LOCUS       DAJSVF010000254          761 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSVF010000254 DAJSVF010000000
VERSION     DAJSVF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.268"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            511..741
                     /locus_tag="ON550_003074"
     CDS             511..741
                     /locus_tag="ON550_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956063.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          278 a          122 c          119 g          242 t
ORIGIN      
        1 tgaatggata tttactttta atagataaaa tattataact attaaaaata actattttca
       61 ttataattac acacaaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
      121 ctaaacacgt ataaacagcg gaagcagaag caactccttc ggaaataagc tgaaattcac
      181 aaaaatttga aaagcaattt ccgtgaattt cttcttattt cttggagtta aacgcttctg
      241 ttccagcctc tttttatctg tctttcacta atgatcagtt gtagataagc aagttgcttt
      301 ttcttacatc ataccgccca tcaagacaaa tgtttttcac ttgtccacac tggacaaaaa
      361 tcccatcaaa tcaaggaaaa gcaacgttgg caatgtttga attttttagg ctagaacgga
      421 ctagtaagag ttttttgccc cttttttgcc cctcaaatat cgctttacaa caagaacgta
      481 agttcgtata atcgttttga ggtgattttt atgatggaag aatttattag aaaaaatatc
      541 agtgatgaat atgcagactt ttatgatcaa agcagtaaaa aagataaatt ccagatggat
      601 gtttcaatct tagctatatt agctttttcc gaaaataaac aacctgtaac tgcaaaaaaa
      661 gaaacagtat tctctgaagg caaaataaaa actcgatata tattagaggt agaaactaat
      721 tttaaaaaca gatcggagta atggttatgc tttttaatga g
//
LOCUS       DAJSVF010000255          750 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSVF010000255 DAJSVF010000000
VERSION     DAJSVF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.269"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>750
                     /locus_tag="ON550_003075"
     CDS             <1..>750
                     /locus_tag="ON550_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR3956064.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFF"
BASE COUNT          324 a          101 c          121 g          204 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt
//
LOCUS       DAJSVF010000256          746 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSVF010000256 DAJSVF010000000
VERSION     DAJSVF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.270"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>746
                     /locus_tag="ON550_003076"
     rRNA            <1..>746
                     /locus_tag="ON550_003076"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          227 a          152 c          221 g          146 t
ORIGIN      
        1 tcagtcaaag agggtaaaaa ccccgtagac gaaatgtgga agacacctag gaggatcctg
       61 agtacggcgg aacacgagaa attccgtcgg aatccgggag gaccatctcc caaggctaaa
      121 tactccctag tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga
      181 aggggagtga aatagaacct gaaaccgtgt gcctacaaca agtcaaagcc cgttaatggg
      241 tgatggcgtg ccttttgtag aatgaaccgg cgagttacga ttgcatgcga ggttaagttg
      301 aagagacgga gccgcagcga aagcgagtct gaatagggcg tttgagtatg tagtcgtaga
      361 cccgaaacca tgtgatctac ccatgtccag gttgaaggtg cggtaaaacg cactggagga
      421 ccgaacccac gtacgttgaa aagtgcgggg atgaggtgtg ggtagcggag aaattccaaa
      481 cgaacttgga gatagctggt tctctccgaa atagctttag ggctagcctc ggaattgaga
      541 atgatggagg tagagcactg tttggactag gggcccatct cgggttaccg aattcagata
      601 aactccgaat gccattcatt catatccggg agtcagactg tgagtgataa gatccatagt
      661 cgaaagggaa acagcccaga ccaccagcta aggtcccaaa atatatgtta agtggaaaag
      721 gatgtggggt tgcacagaca actagg
//
LOCUS       DAJSVF010000257          735 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSVF010000257 DAJSVF010000000
VERSION     DAJSVF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.271"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            101..631
                     /locus_tag="ON550_003077"
     CDS             101..631
                     /locus_tag="ON550_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956065.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(626..>735)
                     /locus_tag="ON550_003078"
     CDS             complement(626..>735)
                     /locus_tag="ON550_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3956066.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          296 a           86 c          111 g          242 t
ORIGIN      
        1 aaatttctaa catcagttat tatgataatt ttgatgatgt caattatgat ttacaggaaa
       61 gatataatga tttaatcgaa gaattagagg agagaggaga atgatttcag ataataaatg
      121 ggaaataaat ctcaaaagat ttatttcaga atatatatat acgattgaaa ctaaatctag
      181 tttttctctt aatgaagaca gtgatcctct aaaaaaatac actattcaat gtaagaaaaa
      241 taaagatgaa aaattgttcg taataagacc agatcttatg aagagtaata cccactatat
      301 aaaagaaaaa cctaaagatt gtgactacat catcataagt gaagcaaata aaacagtatt
      361 tctgatagaa cttaaaagct cagctcagac ttcccctgca aaagaaatta gtgaacaaat
      421 tgatggtgga tataagtggt tacaacatct tagttttcta acaaaatatg attttactga
      481 attcagttat aaaattatta aggtatgttg taaatttgag gcaagacaag atcgtaaaaa
      541 tctttttaaa cctaatgaat atggtattta taatatgaat ggtccaaaat tagacttaag
      601 agagttgtat agaaagtgta acgatctata aattcaaatc ttttttaatt ctatatattg
      661 tttgtctagt aacgttataa ttctttgcaa tctttgaaat agagacgccc tcattcaagt
      721 ccttaacgat gtttt
//
LOCUS       DAJSVF010000258          696 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJSVF010000258 DAJSVF010000000
VERSION     DAJSVF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.274"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>696)
                     /locus_tag="ON550_003079"
     CDS             complement(<1..>696)
                     /locus_tag="ON550_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3956067.1"
                     /translation="MLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTAR"
BASE COUNT          226 a          122 c           91 g          257 t
ORIGIN      
        1 cgtgctgttt ttaaattcaa acctgctgcg taaaaattgt aattaaatat agaaaagcca
       61 tttgtaaatt gggaaccaga aggtgtaaaa tcttgattgt caatatgttg agttgcaata
      121 taagctgttc ttagagcatt agaaatagaa aaatcaacac tacttctatt aatggacgta
      181 aaaaaagttg tagcacaatc cccaatagca tatatatctt ttatatttgt ttgctggtat
      241 tcattagtta gatatgctcc actaacatct ctcaagagtt cctttttgcc aattggacta
      301 ttagatagaa atcctatagc ttgtacgacc atatccatag agtattgggt accacttgta
      361 gtgactattt tttcaatctt ttttcttcct aaatattttt taggactttc tgatagacaa
      421 agatgtattc cattttttga tagtatttct tcaactttat ctgaaaaact acggtcgtaa
      481 taagtcgaca acactctatc tgcaacatcg aaaagatata cttctttccc tctttttctt
      541 atggcttcag caatttcaac tccaatatat cctgctccaa taattcctac ttttttaata
      601 gtattatcat ctaaagcttt aaaaatctct ttagcatttg gaaaattctt tatcgaaaaa
      661 atattagcta aattattccc cggtacatct aacata
//
LOCUS       DAJSVF010000259          670 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJSVF010000259 DAJSVF010000000
VERSION     DAJSVF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.275"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(51..>670)
                     /locus_tag="ON550_003080"
     CDS             complement(51..>670)
                     /locus_tag="ON550_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HCR3956068.1"
                     /translation="KEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDIS
                     ECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFS
                     CNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDI
                     EKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          224 a          114 c           44 g          288 t
ORIGIN      
        1 aactatttaa tactaatgtc ttttataata gcttttcata tattatataa tcaatcttta
       61 taagtccttt tataaatttc ttttctacca ttttcgataa attcctgttt aatattttta
      121 attccataaa caatagtttc aataggataa tattcttcaa ctatatcttg atattctttt
      181 gctttctcaa tatctatatt tccatacatt cttaatatat cttctccaaa atttgttcct
      241 atttcttctt cactatcttc aagtaagtat ataaaatcac aatattcatc tataattcca
      301 gaatctccaa aatcaattat tccagttaat ctattattgc catctaacaa tagatgatta
      361 caactaaaat cattatggca taaacacttt ttaccctcaa aaactgttgt tgcatttagt
      421 ctttccataa aactttctat ataatctttt tctatatcag ttaaatcatt ataaatagtt
      481 tcacgcaaca atatatactc ttctaataca ttttgtttat tatcaatagt acattcacta
      541 atatctgtat aatctaaacc gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac
      601 aaattttgtt cttcttctga catagtagaa taaatttctg gtgttaaaaa agttccttta
      661 atttctttat
//
LOCUS       DAJSVF010000260          664 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJSVF010000260 DAJSVF010000000
VERSION     DAJSVF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.276"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..525
                     /locus_tag="ON550_003081"
     CDS             <1..525
                     /locus_tag="ON550_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3956069.1"
                     /translation="KMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLH
                     EKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQ
                     YKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASIN
                     ILTEGLRLHSMGLA"
BASE COUNT          246 a          106 c          129 g          183 t
ORIGIN      
        1 aaaatggaaa agaaactaaa gcgtgaacaa cgcaaattgt ctaggcgtgc tttattggct
       61 aaaaataaag gtaccaatct atttgaagct aaaaattatc aaaaacaaaa acgcaaagta
      121 gctagattgc atgaaaaagt aatgaatcaa cgtactgatt ttctgaataa gttgagtaca
      181 gaaatgatca aaaatcacga tattatctgt attgaagact taaacacaaa aggtatgttg
      241 cgaaatcata aattagctaa aagtatttct gatgtttctt ggtcaaagtt tgtgactaaa
      301 ttacaataca aggctgactg gtatggacga aaaatcatca aagtagataa atggtttccg
      361 tctagtcaga tttgttcaga gtgcggacat aaagatggca agaaatctct tgaaattcga
      421 gaatggactt gtcctgtttg tcatactcat catgatcgtg atatcaatgc tagtatcaat
      481 attctgaccg aaggtctaag actacattct atgggattgg cttagatcca atcatcaaga
      541 accgtaggaa ctacggggat agcttggtaa ataagagaca ccgctgttag taaagaaata
      601 cgctatcaag tatgctctat tcccaagaag ctcccacttc aagcgttaag aaccgtcagg
      661 ttta
//
LOCUS       DAJSVF010000261          616 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJSVF010000261 DAJSVF010000000
VERSION     DAJSVF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.278"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            51..>616
                     /locus_tag="ON550_003082"
     CDS             51..>616
                     /locus_tag="ON550_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR3956070.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          223 a          103 c          120 g          170 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccac
//
LOCUS       DAJSVF010000262          613 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJSVF010000262 DAJSVF010000000
VERSION     DAJSVF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.279"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            120..>613
                     /locus_tag="ON550_003083"
     CDS             120..>613
                     /locus_tag="ON550_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR3956071.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYG"
BASE COUNT          186 a          123 c          127 g          177 t
ORIGIN      
        1 ttcatagggc ggctttttta tttcaaaatt tccacggtat accgttgact acctcggtat
       61 accgtggtat attatgaatg taaacaaaat atttaatcat ttaataggga gttgaaaaca
      121 tgaaattatt aaacccaaaa actgttatag ctattggaac atttgctgcc acagttgctc
      181 ttgctggatc tgctgacgct gcttcaacta acgtcatgta tcgcctttat aatccaaata
      241 accatgaaca tttttacacc tctagcacaa aagaacgtga tcacctagtg aaaattaagt
      301 ggggtaatta tgaaggcccc gcttgggaag caccgactag cggtggccac cttgtctatc
      361 gtttatacaa taaaggcctt cgtgatcacc attacacagc tagttgggat gaagtgaaat
      421 ggttgaccaa aaattacggt tggacttatg aagggcctgc ctggcgttct gccgaaaaaa
      481 ataataaacc gatctaccgc cttttccttc caggtgttac ctctggttcg catcactaca
      541 ctgcaagttg gaaagaagta caatggttaa ccaaagacta tggctggaaa tacgaaggta
      601 ttggttggta tgg
//
LOCUS       DAJSVF010000263          604 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJSVF010000263 DAJSVF010000000
VERSION     DAJSVF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.281"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            71..>604
                     /locus_tag="ON550_003084"
     CDS             71..>604
                     /locus_tag="ON550_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3956072.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDE"
BASE COUNT          217 a           90 c          137 g          160 t
ORIGIN      
        1 gataaggcga tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga
       61 ggtgaggcga atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc
      121 gcaaattttt attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa
      181 gggaatcaaa aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa
      241 ttcaaacggt ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa
      301 agcaaaagtt aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat
      361 ggctggcgat actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac
      421 atacggagaa ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga
      481 taaatcggta aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga
      541 acttcttgaa aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga
      601 tgaa
//
LOCUS       DAJSVF010000264          597 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJSVF010000264 DAJSVF010000000
VERSION     DAJSVF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.282"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          227 a           76 c           73 g          221 t
ORIGIN      
        1 aaaagtgaaa agagcaaata taaaatttaa taaaaatata gaactaaata ggaaaacgta
       61 gacgtaattt tctacgtttt ccttttcata tatattaaat attacggaaa attatataaa
      121 ctaatttagt gttttgtttg taattcctaa ttttgctcca cttttagata atttttaggt
      181 ctatttaact catcagacat tctatgctca tttttctcca aatttgtttc aactctttcg
      241 tacttaattt tgcgtatttg aaaattcttc ttttagagta cttttattac gatactagta
      301 tgatatatat ctatagcaaa cagttaaaat cttcctaaaa taagcgaata gcattcctaa
      361 aacctataaa atttttagat gaagcgacaa gtggacaatt aataaactac ttgaacatga
      421 aattatggat cacctttcaa atttagaaaa tggaattctt atacttattg cgcaacattt
      481 attgattgca aaattatgtg acaaaaagaa ctagtagaag aaggaatgat ggcttgataa
      541 taaagtacta cctattaaag gtagtacttt atttaattta gtataaatta acatact
//
LOCUS       DAJSVF010000265          594 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJSVF010000265 DAJSVF010000000
VERSION     DAJSVF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.283"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            168..362
                     /locus_tag="ON550_003085"
     CDS             168..362
                     /locus_tag="ON550_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956073.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          251 a           73 c           93 g          177 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg ttta
//
LOCUS       DAJSVF010000266          586 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJSVF010000266 DAJSVF010000000
VERSION     DAJSVF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.284"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..221
                     /locus_tag="ON550_003086"
     CDS             <1..221
                     /locus_tag="ON550_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3956074.1"
                     /translation="NTGLTFSIENGADEVAALELETKVMQDEFGKFYHEAIVAELEED
                     LTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            241..>586
                     /locus_tag="ON550_003087"
     CDS             241..>586
                     /locus_tag="ON550_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956075.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKE"
BASE COUNT          226 a           82 c          116 g          162 t
ORIGIN      
        1 cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct gcgttagaac
       61 tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca attgttgcag
      121 aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat gtgattaaaa
      181 aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg aggtagcgaa
      241 atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa tttagaacct
      301 aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga cgagtttgca
      361 aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc aaaggctgtg
      421 tatattgctt accgccaagc gaacatgact gattatattt cattcgatga atttatcgat
      481 aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat gttcaaacaa
      541 gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaa
//
LOCUS       DAJSVF010000267          582 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJSVF010000267 DAJSVF010000000
VERSION     DAJSVF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.285"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            380..>582
                     /locus_tag="ON550_003088"
     CDS             380..>582
                     /locus_tag="ON550_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3956076.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT          193 a          122 c          118 g          149 t
ORIGIN      
        1 ggaaaagacc tttttaggaa gacacaagtg ccttctcaat tgaaaaacct ttctctcact
       61 ccaaccgcca ccgccgttcg agaagaaagg aagtgaacgc tgcacgttca ctaagatatg
      121 gtgtcttgca aagcaagaca aaatagacaa aacttcctct caccaaaagg ttcgttcctg
      181 ttcgttatct ctttattgat ttgccagacc ggcaacaagt tgccttttat caccaaaaag
      241 cgacaaggac aagggtaaat ggcacgctgc gcgcccttgt ccttgtcgct tttcgttgat
      301 ttattctggc caacaaatca ataaagaaag aactaacgaa aggaagtaaa acctttccta
      361 agaggaggca gaatcaaaca tgcgaaagaa atttgattat tggggtgttc ccttcagcga
      421 attattccca aactatcatg caccacatac tgttgaatgc gattgtggag aaagagcgaa
      481 atgtatcaaa agctatcgtt tgtatcaatg tccaacatgc gggaaaaaat ataccttgtc
      541 ttatggcgat tacattttga ttgatgagaa aggaaagaaa cg
//
LOCUS       DAJSVF010000268          574 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJSVF010000268 DAJSVF010000000
VERSION     DAJSVF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.286"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            138..>574
                     /locus_tag="ON550_003089"
     CDS             138..>574
                     /locus_tag="ON550_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3956077.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAVRIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVY"
BASE COUNT          196 a          110 c          111 g          157 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cacaacattg aaaaatctgg ttttatcgtg tatgattagg gtgtatttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcagt gagaattttc atcgagaaac
      241 aatcgggcgc aacgattact catcgacctg ttttccaaga agcattggat ttcgtccgag
      301 atcaagacat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattaccta aagaagaaaa acgtacagct aatcatcacc agtctaccga
      421 tcatggctga agcaattggc aaccctctcc tggataaatt cattaaggat ctgatcatcc
      481 aaattttggc catgatcgct gaacaagagc gtaccgaatc taaacgacgc caggcgcaag
      541 gcattaaaat tgcgaaagca aatggagttt ataa
//
LOCUS       DAJSVF010000269          561 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJSVF010000269 DAJSVF010000000
VERSION     DAJSVF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.287"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>561
                     /locus_tag="ON550_003090"
     CDS             <1..>561
                     /locus_tag="ON550_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HCR3956078.1"
                     /translation="MFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGKESDR
                     KEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQS
                     ILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEM
                     TAKWESYLLKIGEQKGTQATFLANIQ"
BASE COUNT          228 a           97 c          130 g          106 t
ORIGIN      
        1 ccatgtttgc ggaaaagtat acctatgaag aaacaacgat cttgacgcaa gttcagcagc
       61 ttcagctaaa gacagtcgga aaagtaccag tagatctagg ttggaaaaag ctgtttggca
      121 aagagagcga cagaaaagaa aaggaagaag aaccgctact tcctaaagtg acaaaaggag
      181 aagcggtgac ggtagatcta caagtccttg aaaaagaaac gaagccacca caaccttata
      241 ccgaaggtac gttgatcaca gcgatgaaaa cagctggaaa aaccgtggat agcgaagaag
      301 cacaatccat attgaaagaa gtagaaggaa tcggtacaga agcaacacga gcaaatatta
      361 ttgaaacttt gaaacaaaag gaatatatca aggtagaaaa aaataagctt gtggtgacaa
      421 ataaagggat tttactttgc caagccgtgg aaaaagagcc gctgctaaca agtgccgaaa
      481 tgaccgccaa atgggaaagt tacttattaa agattggtga gcagaaagga acacaagcta
      541 ctttcttagc taatatccaa a
//
LOCUS       DAJSVF010000270          542 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJSVF010000270 DAJSVF010000000
VERSION     DAJSVF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.288"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(71..157)
                     /locus_tag="ON550_003091"
                     /pseudo
     CDS             complement(71..157)
                     /locus_tag="ON550_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          208 a           87 c           52 g          195 t
ORIGIN      
        1 atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg caaccaatag
       61 aaaaaaatac ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat
      121 tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc
      181 ttctattata aaagaagata tatattattt cttaattacc tactacctta aaccaaaatt
      241 taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat cccaattgtt
      301 atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat
      361 atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc
      421 aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact
      481 acaagcatgt agttttatct gttaattatg tactacctaa ataaattagt ttataattaa
      541 ca
//
LOCUS       DAJSVF010000271          533 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJSVF010000271 DAJSVF010000000
VERSION     DAJSVF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.289"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..432)
                     /locus_tag="ON550_003092"
     CDS             complement(<1..432)
                     /locus_tag="ON550_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3956079.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTI"
BASE COUNT          220 a           78 c           69 g          166 t
ORIGIN      
        1 aatcgtaaaa taagactgga tataactaat tacaaaacta gcaaaacttt gtaataacat
       61 caaattaaat acaagttggt tcatatcaaa aaaagcattt gaaaacaaaa aatatatgtt
      121 ccctattaat aagaacacaa ttaacgaaag tgtagctaga gaaagtaccg atgacataaa
      181 ttcatttatg tcatctttat agtcaaaact tgcattatta attgccgaat ttaggcttag
      241 cccaacaaga atcgaaaaaa ttgatacaaa tgaagtgaaa ttattaatca aaccatattc
      301 tgcttgagta gtaatattta caaaaatagg aatagttatg aatgaaattc ccttaataaa
      361 aaaattccct atagtatacc agaaactgga cttaactaat tttgacttgg aaagtgtgtt
      421 aaataatccc atactctacc aactttctta attctgccca gttttctggg agtaaaattt
      481 tttcttctga tagacggtta taatgttcac agaaaagaac aatatttgtc aat
//
LOCUS       DAJSVF010000272          529 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJSVF010000272 DAJSVF010000000
VERSION     DAJSVF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.290"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            33..>529
                     /locus_tag="ON550_003093"
     CDS             33..>529
                     /locus_tag="ON550_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3956080.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          194 a           98 c           87 g          150 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DAJSVF010000273          515 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJSVF010000273 DAJSVF010000000
VERSION     DAJSVF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.291"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..132)
                     /locus_tag="ON550_003094"
     CDS             complement(<1..132)
                     /locus_tag="ON550_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3956081.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          187 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaa
//
LOCUS       DAJSVF010000274          505 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJSVF010000274 DAJSVF010000000
VERSION     DAJSVF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.293"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>505
                     /locus_tag="ON550_003095"
     rRNA            <1..>505
                     /locus_tag="ON550_003095"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          113 c          144 g           94 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagac
//
LOCUS       DAJSVF010000275          469 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJSVF010000275 DAJSVF010000000
VERSION     DAJSVF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.294"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>469
                     /locus_tag="ON550_003096"
     CDS             <1..>469
                     /locus_tag="ON550_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956082.1"
                     /translation="GEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEA
                     TQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDT
                     KYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          190 a           70 c          112 g           97 t
ORIGIN      
        1 caggggaagg aacaagtatt gaaagtggaa caacggtctt tgcagtgaaa gacggcgtaa
       61 gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca gatgcgaaat
      121 ggccagaaga agcaactcaa ccaattacag cagatgatac agaatttgta tcaagtgcaa
      181 caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa aaagtgacta
      241 cagaattgaa taaggaacca gacgcatctg agggaattaa gaacaaagaa gatttaccaa
      301 aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga aataaaaaag
      361 ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta gatgttacag
      421 tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaagg
//
LOCUS       DAJSVF010000276          462 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJSVF010000276 DAJSVF010000000
VERSION     DAJSVF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.295"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          158 a           63 c           64 g          177 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ct
//
LOCUS       DAJSVF010000277          449 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJSVF010000277 DAJSVF010000000
VERSION     DAJSVF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.296"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          150 a           85 c           53 g          161 t
ORIGIN      
        1 gaatgctaaa tcactctaca ataacggcga aagcacaaaa aataaaaaaa ttattacgtt
       61 attaaaaaaa cagatgaaag atcaggtttt attagatctc attctttcat cacttgaagg
      121 gatttaatga actacttttt cctctttgtc gtttttatat attcgtattc cttcaaacag
      181 taggatagcc tctttcaatg cttgatattt gaactgtccg ttacttaaaa atttagatgt
      241 gccgccctct tcctagaaca tattcttttt tactttttca ttaattgaca gaccaatcga
      301 acgaaactgt aaatttcgat aggataatga ttggtcaaac tcattcattt tcaagacatc
      361 tcgattcata ttcaagatag gaatcttttt atcaacatca ctttattcat attttcctct
      421 accataaaag cttcacgctc cacaaaatt
//
LOCUS       DAJSVF010000278          438 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJSVF010000278 DAJSVF010000000
VERSION     DAJSVF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.298"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            11..>438
                     /locus_tag="ON550_003097"
     CDS             11..>438
                     /locus_tag="ON550_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3956083.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          167 a           57 c           87 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagc
//
LOCUS       DAJSVF010000279          425 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJSVF010000279 DAJSVF010000000
VERSION     DAJSVF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.299"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>425
                     /locus_tag="ON550_003098"
     CDS             <1..>425
                     /locus_tag="ON550_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR3956084.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          134 a           71 c           85 g          135 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacac
//
LOCUS       DAJSVF010000280          416 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJSVF010000280 DAJSVF010000000
VERSION     DAJSVF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.300"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          190 a           54 c           66 g          106 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcg
//
LOCUS       DAJSVF010000281          416 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJSVF010000281 DAJSVF010000000
VERSION     DAJSVF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.301"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            123..>416
                     /locus_tag="ON550_003099"
     CDS             123..>416
                     /locus_tag="ON550_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3956085.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTND"
BASE COUNT          161 a           63 c           68 g          124 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgat
//
LOCUS       DAJSVF010000282          394 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJSVF010000282 DAJSVF010000000
VERSION     DAJSVF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.303"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..285
                     /locus_tag="ON550_003100"
     CDS             <1..285
                     /locus_tag="ON550_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3956086.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DAJSVF010000283          383 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJSVF010000283 DAJSVF010000000
VERSION     DAJSVF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.denovo.304"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..174)
                     /locus_tag="ON550_003101"
     CDS             complement(<1..174)
                     /locus_tag="ON550_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3956087.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQ"
     gene            complement(278..>383)
                     /locus_tag="ON550_003102"
     CDS             complement(278..>383)
                     /locus_tag="ON550_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3956088.1"
                     /translation="SVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          104 a           58 c           64 g          157 t
ORIGIN      
        1 ttgtcggttt tctgttcgtt catattcttt tgcttgaata tattctgttc gttgattttc
       61 cattagttga ttaaataccg ttgttaaaat atttttagaa acgtcatcct ttacagaata
      121 ttcaataatg ctttgaatct cttcgctttt cagtgtaaaa tgtacttggg tcatgtaaaa
      181 gtcctcctgg gtatgttttt gtcgttaaaa acattgtacc gtaaaaggac tgttatatgg
      241 cctttttact tttacacaat tatacggact ttatcaatta agcgagacaa aactttccat
      301 ttacgtaact caaaaaaact tctaatggtg tttggtaatt tagtgatttt ctagggatat
      361 ggtttctttt ggacgcaacg gat
//
LOCUS       DAJSVF010000284        33393 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.025, whole genome shotgun sequence.
ACCESSION   DAJSVF010000284 DAJSVF010000000
VERSION     DAJSVF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.025"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..549
                     /locus_tag="ON550_003103"
     CDS             <1..549
                     /locus_tag="ON550_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCR3956089.1"
                     /translation="DLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVIN
                     FSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENL
                     SKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKR
                     EAKLIIGISSTVTDYISGYLDK"
     gene            616..1110
                     /locus_tag="ON550_003104"
     CDS             616..1110
                     /locus_tag="ON550_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3956090.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1166..1804
                     /locus_tag="ON550_003105"
     CDS             1166..1804
                     /locus_tag="ON550_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956091.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1876..2271
                     /locus_tag="ON550_003106"
     CDS             1876..2271
                     /locus_tag="ON550_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3956092.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2255..4708
                     /locus_tag="ON550_003107"
     CDS             2255..4708
                     /locus_tag="ON550_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3956093.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4705..6732
                     /locus_tag="ON550_003108"
     CDS             4705..6732
                     /locus_tag="ON550_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956094.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6729..7751
                     /locus_tag="ON550_003109"
     CDS             6729..7751
                     /locus_tag="ON550_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3956095.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7768..8697
                     /locus_tag="ON550_003110"
     CDS             7768..8697
                     /locus_tag="ON550_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3956096.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8942..9058
                     /locus_tag="ON550_003111"
     CDS             8942..9058
                     /locus_tag="ON550_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3956097.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9074..9877
                     /locus_tag="ON550_003112"
     CDS             9074..9877
                     /locus_tag="ON550_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3956098.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10112..10348
                     /locus_tag="ON550_003113"
     CDS             10112..10348
                     /locus_tag="ON550_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956099.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10355..12307
                     /locus_tag="ON550_003114"
     CDS             10355..12307
                     /locus_tag="ON550_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR3956100.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12379..12876)
                     /gene="dfrG"
                     /locus_tag="ON550_003115"
     CDS             complement(12379..12876)
                     /gene="dfrG"
                     /locus_tag="ON550_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR3956101.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            13238..14278
                     /locus_tag="ON550_003116"
     CDS             13238..14278
                     /locus_tag="ON550_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3956102.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            14376..14564
                     /locus_tag="ON550_003117"
     CDS             14376..14564
                     /locus_tag="ON550_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3956103.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(14622..14975)
                     /locus_tag="ON550_003118"
     CDS             complement(14622..14975)
                     /locus_tag="ON550_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3956104.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            15506..15928
                     /locus_tag="ON550_003119"
     CDS             15506..15928
                     /locus_tag="ON550_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3956105.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            15925..16155
                     /locus_tag="ON550_003120"
     CDS             15925..16155
                     /locus_tag="ON550_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3956106.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            16653..16853
                     /locus_tag="ON550_003121"
     CDS             16653..16853
                     /locus_tag="ON550_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3956107.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            16880..18073
                     /locus_tag="ON550_003122"
     CDS             16880..18073
                     /locus_tag="ON550_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3956108.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(18141..19706)
                     /gene="guaA"
                     /locus_tag="ON550_003123"
     CDS             complement(18141..19706)
                     /gene="guaA"
                     /locus_tag="ON550_003123"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3956109.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(19765..20688)
                     /locus_tag="ON550_003124"
     CDS             complement(19765..20688)
                     /locus_tag="ON550_003124"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3956110.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            20884..21489
                     /locus_tag="ON550_003125"
     CDS             20884..21489
                     /locus_tag="ON550_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3956111.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            21797..22813
                     /locus_tag="ON550_003126"
     CDS             21797..22813
                     /locus_tag="ON550_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3956112.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            22954..24255
                     /locus_tag="ON550_003127"
     CDS             22954..24255
                     /locus_tag="ON550_003127"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR3956113.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            24301..24963
                     /gene="deoC"
                     /locus_tag="ON550_003128"
     CDS             24301..24963
                     /gene="deoC"
                     /locus_tag="ON550_003128"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3956114.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            24984..25382
                     /locus_tag="ON550_003129"
     CDS             24984..25382
                     /locus_tag="ON550_003129"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3956115.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            25448..26530
                     /locus_tag="ON550_003130"
     CDS             25448..26530
                     /locus_tag="ON550_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3956116.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            26742..28304
                     /locus_tag="ON550_003131"
     CDS             26742..28304
                     /locus_tag="ON550_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3956117.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            28297..29430
                     /locus_tag="ON550_003132"
     CDS             28297..29430
                     /locus_tag="ON550_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3956118.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            29430..30386
                     /locus_tag="ON550_003133"
     CDS             29430..30386
                     /locus_tag="ON550_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3956119.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            30485..31651
                     /gene="deoB"
                     /locus_tag="ON550_003134"
     CDS             30485..31651
                     /gene="deoB"
                     /locus_tag="ON550_003134"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3956120.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            31675..32493
                     /locus_tag="ON550_003135"
     CDS             31675..32493
                     /locus_tag="ON550_003135"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3956121.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            32510..33217
                     /gene="deoD"
                     /locus_tag="ON550_003136"
     CDS             32510..33217
                     /gene="deoD"
                     /locus_tag="ON550_003136"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3956122.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11012 a         5809 c         6878 g         9694 t
ORIGIN      
        1 gatttagact tttttgaaag tcataaagat ttgattaccc agacagcaag ctccatatat
       61 gataaacaag gtgataatgt attagtggat actattttag taccacttcc agagaattac
      121 gaagtaatta actttagtca gttgaaaatt agtgatgtgg tttcacaagc tattgaagac
      181 gctgagtctt ttatgagtaa tggtgaatat caacgtgcct ttgacagagt tcacactgct
      241 tttcatggat atttaattga aatactaaaa aaatatgaaa ttacagttcc tagagacgaa
      301 aatctatcta aattatatag taggatacaa caattaattg aaaaagaaat tcaacctact
      361 gaacttgctg atatagtgaa gacaacgata aggtcatcaa atggaatgat tagctctctt
      421 aatgaagcaa gaaataggca ttcacttgca caccctaata caaatattat tggaaagaga
      481 gaagcaaaac taataatagg tatttcaagt acagttacag attatattag cggatacctt
      541 gataagtagc gtcattaact gattgctact ttttttatga ctatttttat aaaaaggatg
      601 tgataagaat tggaaatgaa agtctatgtg gcaaatttag gacggtataa tgaaggtgaa
      661 ttagtcggag catggtttac tccaccaatt gatgaggaag aaatggcaga aagaatcggt
      721 ttaaatgagg actacgaaga atatgcaata catgattttg agcttccttt tgatgtggac
      781 gaatacacgc cgattagtga aatcaaccgt ctatgtgaag ccattcaaga aatcgaaggt
      841 acacctattt ataatgaact caaagaaatt caaggcatgt ggtttagtag cttggaagaa
      901 ttactggaaa acaaagagga tattcattgt tatagtgatt gtgattcgat ggaagatgtc
      961 gcccgttatt acgtggaaga aacaggacag cttggtgaag tcccttccaa tttacaaaat
     1021 tatattgact accaatcttt gggacgtgat atggaaatag agggtaatta cctagttacc
     1081 tctcatgggg tatttgaata ttgtcagtaa gtttaacaat ccgtgttata attaaattaa
     1141 taaatagcac ggagaggtat aaattatggt aaatgtattt gaaaatctta aactattaaa
     1201 actagacatg aaagataaag gctgggtgat agattccttt tactttcgtt acaaacagca
     1261 aaattatatt gtacttgtta agctatttga aaaagaggaa aaagttccag aatatgctct
     1321 gttaaaacta gaatttttaa aggaaaacga tttttcagat atgctagctg tctatgctaa
     1381 ttcggtaaaa ttatatacag acacgaaaac aataagagaa tattttggta tcgaatacag
     1441 tagtaacttg ggagatgttt tatttcaatt tagccaaact cttgctcgat ttattccgac
     1501 agaagttagt gagaagaaaa atgaagacca aaaagaagcc atgtgtttct cattaagtca
     1561 aagtgattca gaagatccca gaaaaaaata ttgtttttca gttagaagaa atcctttaaa
     1621 gggaaatggt gagctgggaa aacgaagtcc ttttaatgac aataaaacga gattgtatag
     1681 acccagctta tatgaacgat tagggtcaga taccaattta agtttcagat attcaatgaa
     1741 tccagatgat gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa ataaaattga
     1801 atagttaaag cattgaaaga gcaatcacct attttgattg ttctttttct atttttacgg
     1861 aaaggacgtg aagacatgaa aaaaataaga agttacacca gtatttggtc agtagaaaaa
     1921 gtgatttatg cgattaatga tttacaactc cctttcccaa ttacctttac gcaaatgaca
     1981 tggtttatcg tttctttgtt tgtcgtcata ctctttggca gtttaccacc actttctctg
     2041 attgacggag cactactcaa atacttaggg attcccattg ttctcactgg gttcatgtgc
     2101 caaaagacct ttgacggaaa gaaaccctat ggatttctaa agtctatcat tacctatgct
     2161 ctacgcccta aagtaaccta cgctggcaaa caaatacgat taaaaaaaga aacaatgaat
     2221 gaatcgatta cgattgtaag gagtgaacaa ctagatggca tatccgatta aatatatgga
     2281 agataatctc gtatttaatc atgatggcga agtattcgct tattatgaat taattcccta
     2341 taattacagt tttttaagtt ccgatgaaaa aattcaagtt catgataatt tctgtcaatt
     2401 gattgcccaa aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg aatcaagtat
     2461 tcgagccaca caggaacaat cgaaaaaaga aatcactgga cgattaaaag agatagctta
     2521 cgaaaaaata gatgaacaga ccgaagcgtt aatttctatg attggtgaca atcaagtaga
     2581 ctatcgcttt tttattggtt ttaaactcta cctcaatgaa caggaagtat ccgtaaaaaa
     2641 tgttgggaaa gaaattcaaa aagcatttgc ggaattcttt catggtgtga atcacaaatt
     2701 aatgggcgat tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta agatggaaca
     2761 gttgttagaa agtaaattat ccagacgctt tcagattcgt aaattggaga aagatgattt
     2821 tggttatatt attgaacatc tttacgggca aacaggtgtc ccttttgatg actacgacta
     2881 ccatttaccg aaacgatacg aagataaaaa agtgttggta aagaaatacg atattctcaa
     2941 acccactcgc tgtttaatcg aagaaaatca acgctattta cggattgaac aggaagatac
     3001 cactacttat gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg atttccctaa
     3061 tagtgaaatt ttttattacc agcaacagca gtttactttc cccgttgata ccagtatgaa
     3121 tgttgaaatt gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa agaaagaact
     3181 gaaagattta gatagccacg catgggaaag cgacaacgaa accagtacta atgtggtgga
     3241 cgctttagaa tctgtcgatg aattagaaag taatttagac caaaccaaag aatctatgta
     3301 caaactaagc tatgtggtac gggtatcagc aaagaatttg gacgagctga aacaacgttg
     3361 tgatgaagta aaagattttt atgatgatct gtctatcaaa ttagtacgac cttttggcga
     3421 tatgctaggc ttgcataatg aatttattcc ctcaagcaag cgatatatca atgactatat
     3481 tcaatatgtc acgtcggatt ttttggctgg tcttggtttc ggtgctacac aaatgattgg
     3541 tgaaaattct ggtatttacg tcggttacaa tctcgataca ggcagaaata tttatctcca
     3601 acctagcctt gcaagtcaag gaattaaagg ctctgtaaca aatgcattag cgtccgcttt
     3661 tattggttca ttaggtggcg gaaaatcatt ctctaacaat atgctggtct attattcagt
     3721 actctttggc ggacaggcaa ccattgttga cccaaaagct gaacgtggtg aatggaaaga
     3781 aaccttacct gatattgccc atgaaatcaa tattgttaat ctcacgagtg agaacgacaa
     3841 taaagggtta cttgaccctt atgtgatttt aaaactcccg aaagattcag aaagccttgc
     3901 cattgatatt ctcacgttct taacaggtat ttccagccga gatagcgaga agtttcctgt
     3961 cctgcgtaaa gctattcgga gtgtaactca aagcaaacaa cgagggttgt tattggtgat
     4021 tgatgaattg cgaaaagaag atacgcccat cagcaatagt attgctgacc atatcgaaag
     4081 ctttgtcgat tatgattttg cccatctgtt attcagtgat ggcagtgtca cccaatccat
     4141 tagcttggaa aaacaactca atattattca agtggcagat ttggtcttac ccgattccaa
     4201 tagctctttt gaagaataca ctaccatgga attattaagt gtggcaatgc tgattgtcat
     4261 tagtaccttt gctttagact ttatccactc tgaccgttcc atttttaaaa ttgttgacct
     4321 tgatgaggct tggagttttt tacaagtggc acaaggaaaa gctctatcta tgcgattggt
     4381 tcgagcagga cgtgccatga atgcaggggt ctatttcgtt actcaaaacg cggacgattt
     4441 actagatgaa aagatgaaga ataatattgg cttgaaattt gcctttcgtt ccactgacct
     4501 agtggaaatc aaaaagacct tagaattttt tggtgtggac aaggaagatg aaaacaatca
     4561 gaaacgctta cgtgaattag aaaatggaca atgtttagtt tcagacatct atggtcgtgt
     4621 aggggttatg cagtttcacc cgatttttga agagttgctt catgcctttg ataccagacc
     4681 gcctgtgcga aatgaggtgg aagaatgaca caaaaacaaa agaaatttct tcactatagt
     4741 tggataacgc tcctttgtgt gggcgttatt ttacttttat taggcacgct gggcaatgca
     4801 gtacaagcga caggcttagt tgatgaaacc attgatacaa gcaatgaata ctccaagtat
     4861 ggtttgaatc aatatcagct tgattattat gtggacaata gctggggctg gcttccatgg
     4921 aattggagtg atggcatagg acaatcggtg atgtatggat tgtatgccat tactaatttt
     4981 atttggacaa tttctcttta tctatcgaac gccacaggtt atttggtgaa agaagcctat
     5041 gccttagatt tcattagtca aacatcccaa gcaattggcg aaaacatgca gacgattgca
     5101 ggcattacac cgaatggctt ttccagttca gggttttatg tcggtttcct ccttcttttt
     5161 attctcattg ttggaatcta tgttgcctat acaggattgg ttaagcgaga aacgacgaaa
     5221 gtgatacgag ctgttttaaa ctttgtcgtg gtgtttattc tatcggcttc ctttattgcg
     5281 tatgcacccg attatattgc gaaaatcaat gatttttcca aagatgtcag taccgccagt
     5341 ctgaatattg ggacaaaaat tgttcttcct aacacggata gtcaagggaa agacagtgtg
     5401 gacatgatac gtgacagctt attctctgtt caagtaaagc aaccatggct attgcttcaa
     5461 tatggctcca ctgatgtaga agcgttaggg gaagaacgtg tggaaagttt attgtctacc
     5521 agtccagata caaacaatgg tgaagataga gaaaatgtag tgattgaaga aattgaagat
     5581 catgacaata cctatttaac cctaccgaag acgatttcca gattaggaac agtcttcttt
     5641 ttgtttgtat tcaatattgg tatctcgatt tttgttttcc ttctaacagg aatcatgatt
     5701 ttttcgcaag tgctatttat tattttcgct atgtttttgc caatcagttt cctattaagt
     5761 atgttgccga gttttgatgg catgggcagg catgccatta ccaagttatt taatgtgatt
     5821 atgacaaggg ctggtattac cttgattatt accgttgcct ttagtatttc cactatgctt
     5881 tattcgctgt cagcaagttc tccctttttc atgattatgt ttttacaaat tgtgaccttt
     5941 gcagggattt attttaaact gggcgattta atgagcctat tcactttaca aagcaacgac
     6001 tctcaaaaca tgggcagaca agtgtttcgc agacctcgtc aaatgatgaa tcgacaaacc
     6061 agaagatttc aacgcagtat gagtaagatt tttcgtggaa aagcagggtc aacgaagcaa
     6121 aagacaatga caacaaagca gaatcaaacc aataaagcta accatactcg tcaaaatgaa
     6181 cggactacac caaagaaaga aacagcactt tccaatgtga aaaagcaaca aggcggtatc
     6241 gggcaacgat taggtgaaaa gacagcccat gtattggaca cgaaaaatcg tctagtcaat
     6301 aaaagaaagc aggcaaaaca acagatacag gcgacaccaa cccatgtcaa atatgccctt
     6361 catcaaggaa aagagaatgt cactcataat gtaacagact ttaaacaatc tatggcagat
     6421 accaaagctc aacgcaaaca acagcgtatg aaccagcagg agcaaaaacg aaagaacatt
     6481 gctcaaaagc gtttggagct ggataaagca aaagaaaaaa gacgaaaaga aaacccatca
     6541 tctcctgttc agaataacca gcagcatgtt cgcaagcgtc agcaaaccgt cacgcctgtt
     6601 ttgtcacaaa acactaaacg tgaaaatcat cagcgaccat tgaaaaaaga gcaaaaacaa
     6661 aagacaccac cgaagcaatt caatcagagg aagctaaata aaagaacgga taagcgagga
     6721 caacaaacat gaagctaaaa ataacagtga tttgtagtgt ggtcttctct ctcttctttt
     6781 ttctcttatt attcctagtc attttctttg cagatgatac ggacagtggc gaaaacaaca
     6841 aggattcttc tattccacaa ggcggtgtga ccgtttcacc agaagtgttg gcacaccgtc
     6901 cgttgattga aaaatatggt aaggaatatg ggattgaaga ctatgtttct tatatcttag
     6961 ccattatgca agtggaatcg ggcggtacag cagaagatgt tatgcaaagt tccgaatctt
     7021 tgggtttacc gcccaacagt ttgagtaccg aagagtccat caaacaaggg gttaaatatt
     7081 tcagtgaatt attaaccagt gcagaacaac aaggagtgga tatagattct gttatccaat
     7141 cttataatta tggcggtggc tttttaaatt atgtgaggag tcatgggaaa aaatatacct
     7201 atgaattagc cgaacagttt tctaaagaga agtcgggtgg tcaaaaagca gactatccca
     7261 atcctatagc catacctgtg aatggtggtt ggcgatataa ctatggaaat cagttttatg
     7321 ttcagttggt ttcacaatac ctaaccgaca cttccccaac agaatttgat gatgaaaccg
     7381 tgcaagtcat tatggacgaa gccttgaaat acgaagggtt tccctatgtc tttggagggg
     7441 cgtcgcctac cacctctttt gattgtagtg gcttaataca atgggtgtat gacaaagctg
     7501 gcatttctct ccctcgtgtc gcccaagatc agtatgacgc tacgcaagaa atctctatgg
     7561 aagaagccca agcaggcgac cttatcttct ttcattcaac ctataatgca ggcacatatg
     7621 tgactcatgt ggcgatatat ttagagggaa atcgctttta tcatgcaggc gacccaatcg
     7681 gttatggcga cttaagcagt cgttattggc aagaccatct gattggggct agacgtgtta
     7741 ttcataacta agaaaggaat gaatcaaatg attataaaaa tcgagcgaaa agaaaagaag
     7801 gagaaaccaa agaaatctaa aaaaccgagc aagcaaagaa aagttccgat gattaaagtt
     7861 ggcacgcata agaaattaac ctttgtttta tgggttttat tgattggtag tgtcggattt
     7921 ggaatctata aaaattttac tgccattgac actcatactg tccgtgaaac agaaatcatt
     7981 aaacagcaga ttgtcgatac caaccaagta gaaagttttg tgaaatcctt tgcgaaagac
     8041 tacttttcgt ggcaacaatc ccaagaagcc attgataaac gaaatgaaaa attgacccat
     8101 tatttgacgg aagaattgca ggtattaaat gaagaaatga ttcgtaagga tattcctact
     8161 agttctagtg tgaacgatat acaggtgtgg caggtttctc aagtgaatga gaacaccttt
     8221 gaggtactgt tttctgttga acaagttatt accgaagaca aagacaagga aactatatcg
     8281 tctagctttc atgtggttgt ccatatagat gaatcagata atatggttat tatcaaaaat
     8341 ccgacaatga gtaagaaacc tcaaaaatcg gactaccaac ccaaacagct agaaagtgac
     8401 catacagtag atacagaaac gatggacgaa atcactagct ttttagaaac gttcttccag
     8461 ctctatccta ccgccacaga aaaagaattg acttactatg taagtaatca tgtgttacct
     8521 atgattaaca aggaatatgt tttcgaggaa ttggtgaatc ctatctttac cagaaaagat
     8581 aaccaagtaa tcgtgaatgt cgctgtgaaa tatttagatc aagaaacaaa ggcaacccaa
     8641 atctcgcaat ttgagttgat attagaaaaa caagataatt ggaagattgt aaaataacaa
     8701 atattggtac atgattacag atactttgta atcatgtact ctttttgata aaaaattgga
     8761 gattccttta caaatatgct cttacgtgct attatttaag tgactattta aaaggagtta
     8821 ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagcggg aacaaataat
     8881 tagatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca
     8941 tgtgattcta aagtatccag agaatatctg tatgctttgt atacctatgg ttatgcataa
     9001 aaatcccagt gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat
     9061 ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa
     9121 aactacctta acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt
     9181 ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat
     9241 tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc
     9301 aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat
     9361 tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact
     9421 taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa atggaattga
     9481 tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca
     9541 gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga
     9601 tacggtaata gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga
     9661 agcattggaa ctcgaacaag aggaaagcat aagatttcag aattgttctc tgttccctct
     9721 ttatcatgga agtgcaaaaa gtaatatagg gattgataac cttatagaag ttattactaa
     9781 taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat
     9841 tgaatataca aaaaaaagac aacgtcttgc atattaagta gttcagtttt ggagtacaaa
     9901 aagtgaaaat gtaaacctaa atgagtatca aattgcaact gaatttgata ctcgtttttt
     9961 attgttaaat caacgtttgt tgttaattta caccacaaaa tgtaagttaa aatgtatata
    10021 aaaatgcgaa cgtgaaataa ttattgtagg aatgcaaatt ggtgtgttaa aatggttaca
    10081 atttactaag caaaaagggg tgcattttcc tatgcaattt cttgagccaa aaaataagaa
    10141 cgctaagtct gtggattggg aaatatcaga gcaagtaagg gtgatagtta agcaatacgc
    10201 tgaatatgca gagcgtactg aaagcgaagc agtggatgaa ttcttattga acattttaga
    10261 cgataagaag tttattgaat ggattgccaa caagcgaagt aataagcgga ttgttgaaaa
    10321 aatgggtatc aaagatagag tgggttgata gacaatgacc aagcttaaaa gattagccac
    10381 gaaggaagat gaaattgtag atgttaaaat tccgatttca aacgaagaat taaaagatag
    10441 ggctaaacaa tatgatttat taactccaaa acgatttgca acaaggtata acaaaatgtt
    10501 gttcttgcca acgtctttta aatggaacgg ttctgaatat cccattcaat ataattactg
    10561 catcaatccg ttttgttgta attttgggaa agaacagcac aaattcaaag atgtaaaagg
    10621 gaaaccaagt cgatataaaa tgactggtag ttccaaagat aaaggtcata aaggtatgta
    10681 ctgcaatgac aatcctattg gcagaggtgt atcgcaaaac tgtacagtaa cccctttatc
    10741 taattggtct gttgttgaag aaattaaacg ccttatagaa atcaatagca tccaagatgt
    10801 tgagccagat tatcaatttc ataaagaagg ctgctcggaa gaagaatcaa ccccattcaa
    10861 tgagccgaag caattttaca aaagggggaa aagtaggggc aaatctcaac gctatcaatg
    10921 taaggcgtgt aagaagttca ctaatgttct accaaaaaga gaagaaacca ctacttatca
    10981 ccaacaaaag aacaccattc ttccaatgtt cgctaagatg gttgttggaa gggtttcagt
    11041 cagtcgtact tgtgatattt taggaattgg agttggaacc tactatcata agctggagtg
    11101 gctgtatagg cgttgtttgg agtttttaga acgatatgag acacaacccc ttcaaacaaa
    11161 gaaatttaac gagatgtggc ttaatacgga caagatgcac tactacctaa ataacgtccg
    11221 taagaaagga caaggttcaa agaaatatac ggggtttgaa gaccttaata tgcaaacgta
    11281 tatagttgta tctgctgagg tcttatcaag atatgtgttc cgttctgatg ttgcctatga
    11341 ttggaatatt tcaatggatg aattaaatga agatacaaga aaattcaaag aagaccatct
    11401 caatactttt agtcgtaaaa atgataggtt agattggtct tattatccac aagaaccatc
    11461 cgctaacgat agcgaaaata ggaatgctta tcttcacgaa ttagggaaaa ttactaatag
    11521 gagtagattt gtcgatggtt taaatgttga tgctccttat actacaactg ctcactattg
    11581 gttaattaaa caaatggtta atgccgacga atggcgaatg attagtgatg atgacttctc
    11641 tattagaaac gccttttata gagtattcac gaaagaatta aggttgagcg atgcacatca
    11701 ttttatttgc caagttaaca agacgaaaag cagaaaacaa tgcttaaaag aattcggaca
    11761 agcaaaagca gaactgctcg attggggaga tatcagagga ttcaaaacaa agtttctacg
    11821 gactttggca tctcattatc taactgaatt actaacaagt catcaattcc acgaagaggc
    11881 tattagtaaa gatggagaaa gatatcgcaa atatgccgat aaccctatca aacatccatt
    11941 agccactaag gataaaggat tttattcagt tgattgtaga acggatttat ctgctctgga
    12001 accaaacgaa atcgcaaaga tgctattaaa tgttaatgac cattcgacca atagctttat
    12061 tcaacagata agacgttata tatcatctct tgaaagacca ttaacaacag ctcgtggtga
    12121 taagaagagc tatatttacg ctaacttcaa tccgaagtat gctcaatttg cgattacaat
    12181 tctaagaact tactacaact tttgtcgccc ttttaaatca gcggataaaa aggttttgac
    12241 acctgctcaa agattaggga ttacagataa acaatttgat tggaaagata taatttattt
    12301 caaatgacaa aaagagcttg gagtaatccc aagctcttgt gctttatctt tactgttctt
    12361 cttcaattaa cgcacccgtt aactcaataa gttttttctt tcatatacat gaaaatagta
    12421 gttatacgga tttttatcat tctttatccc tttttgggca aatacctcat tccattcctc
    12481 ataattcact tctggaaaaa aagtatctcc ttcgaattca tgatgtattt ttgtgatgta
    12541 cattttctca acataaggga aaaacaaatt ataaatctgt tctcctccga aaataaaaat
    12601 ttcttcttcg tttttacata actcaaaaac atcttctatt gaatgaacaa tttcacaacc
    12661 attaaaggta aaccccttat ctctcgtcag aataatattt cttctgtcag gtaaggctct
    12721 tccgattgat tcaaggttct tcctacctaa tattatcgga tgtccctttg tagtattttt
    12781 aacatattcc cagtccttgg gaatcctcca aggaatgtca ttctctttgc caatcactct
    12841 attcttatcc atcgcagcaa tcaaagaaac tttcatttat aacacagcct ttttaactaa
    12901 tttaccatct aatttcttat ttctatcaaa aaaccttttc tcattatcct gcccgttagt
    12961 tgaataagca attttttcta taagttatat tatcataata ccacaaaatc acaaattttc
    13021 attcaattat cattttcaat tgcattttat aaatcatttt gtatgtaatt tggtatgtaa
    13081 gttaaaatga aaaaagtagt ccattttgaa gtacattttg tcattttgta ctccaaaact
    13141 gaactactta cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt
    13201 tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga
    13261 attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt
    13321 gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa
    13381 tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt
    13441 gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc
    13501 tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc
    13561 actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat
    13621 ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt
    13681 ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga
    13741 gagctcggtt tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat
    13801 acgctatggt tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt
    13861 taagtacggt ttatactata gccctgttag tactccagca gattttcgga tgcttactcc
    13921 tattgtactg gagcaagcct ttagaaaagc tggaacagaa ttgttagagc catatcttag
    13981 ttttaaagtt tatgcaccac aggaatatct ttcacgggca tataacgatg ctcccaaata
    14041 ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag gtcattatta ttggagaaat
    14101 tcctgctcga tgtattcaag attatcgcaa tgatttaact ttttttacaa atgggcttag
    14161 tgtttgttta gcagagctaa aaggatatca ggttaccact ggcgaacctg tttgccagac
    14221 ccgtcgtcta aatagtcgga tagataaagt aagatatatg ttcaataaaa taacttagtg
    14281 cgttttatgt tgttatataa atatggtttc ttattaaata agatgaaata ttctttaata
    14341 tagatttgaa ttaaagtgga aaggaggaga ttgttattat aaactacaag tggatattgt
    14401 gtcctatttg tggaaataaa acaagactac gaatacgagt ggatactata cttaaaaatt
    14461 tccctttata ctgccccaaa tgtaagaacg aaactttaat taatgttcaa aaaatgaata
    14521 taataacaat caaagagcca gacgccaaga cgcagagccg ataatttgag aaatgaaact
    14581 ctcatcttat cggctctttt tgtttatctg aattttactg actagccttc aatatttctt
    14641 gcttcattga taccttttgc tagtgcttcc attaaggata gttctttgtc tgtaaagcta
    14701 tccatgtatt tttctacctg taaccttcga gtgcttttaa tcaaattatc tgtaggtaag
    14761 aaaaattcat caatagatac atggagtaat gatacaagat catacagcac ttgtgtactt
    14821 gggtgttgtc ctttgttttc tatatttgtt aaataacgtg ggtcaatttc aatcattgtt
    14881 cccacttgtt cacgagtgag accccgtttc attcgagcct ctttaatcgc taaaccaacg
    14941 gctctaaaat cgtatttatc ttctttttta cgcatactaa atcacctcta tacattttac
    15001 tgttcctata taaccataaa caggtataga aaaacatgtt atatagttta tgagttccta
    15061 tagaacactg tggaatttat taatgcgatg aatccaattt caaatagaaa ggtcgctgat
    15121 tttatttacc tatacaactt tataaaattt agtcatactc ctagaaagta gataaaattg
    15181 gcgacttaat ggagggaaaa gtcgccatga gaaagataat ctgtcatcta gttctcttaa
    15241 ttaagttctt atcaaaaaaa cttatccacc tgtactggat agatatgacc atcaatgtgg
    15301 ttttgcagat tacatagata gaaaaatcaa agcatatgaa cagagaaaac aatctgttta
    15361 tgtgcttttt tggtcactta ataaatatct tttaggattt ctcttcattt atgcaacaaa
    15421 tcccccgttc gtgtcggggt ttagtatgaa gggagataaa gaagcacatg ccactccctt
    15481 tcacgacaga aagggggtga aaagcatgaa accatcttct tttcaagaaa ccatagaaaa
    15541 tcagtttgac tatatctgca aacaagttat tgaagatgaa cgaaaagatt acttcaaaca
    15601 tctagacaga ctatcagcaa aagaagtctc gttttctgat atagataact accttgtcaa
    15661 ctctttttct actgttgata cgtacgtcac tgatttccaa ctatttaaat tatttggatt
    15721 aagaatcaat gttgaaagtg atttattaag tgaagccctg cgtcatcttt cagagaaaaa
    15781 acggaacatt attttacttc attactttat ggatatgagt gatgtggaga ttgcggagtt
    15841 actaaatttg aatcgttcga ctgtttatcg tcatcgaata agtggactag ccatgattaa
    15901 agaattcatg aaggagtgta aagaatgaaa acagcttatc caagggttcc cttccccctc
    15961 attgtaaagg cgacagatgg cgacgtggaa gcaattaatc agattgttaa acattatcga
    16021 gggtatacct caaaacgttc attacgtcgc atgacagacg aatatggaaa ttctcatatg
    16081 gttattgatg aaaccctacg tggcagaatg gaaacgaaac tcatctctaa gattttatcg
    16141 ttcgaaatta aatagtccca ttttttcgtg gaagcgtgtc gaatcgtatc cacgcttccc
    16201 gatttgggag gtttgttatt ccatcaaagc atattgtgtg ttcagtaggt tttgataggc
    16261 taacaagcca aacgttcttt gacaaccaaa taaaggcaat cgaatacgtt tcggtaagaa
    16321 aagagctgat gaactaacac gccatgacct acaaaattag tgagcgattc atgttagtaa
    16381 ttcgaaagta atctttagca ggattgctgc catgactttc ttatcgtgat aatgatactc
    16441 ccgtatagcc atagtccgag cgttagaagc gtcacaggca atgggtacgg ctacatgaga
    16501 accatgcaga agtgaaattc ctgtgagctt tgctagagct gttcgattgc taagatattc
    16561 ttctcttgtc agtgtattat tataaaacta tttgatataa ttataggtga atattggagt
    16621 tttcctagcc tataattggg aaaggagtaa taatgataac ggtcacaaaa aaggatttag
    16681 ttgagctagg atatggaact tctttttcag cagacatcat aaaaaaagca aaagaattga
    16741 tgattacaaa agggcatatc tactatcaat ctcgaaagct agatagagtc cctcgtgaag
    16801 ctgttgaaga aattctagga attcggtttt caaatgaaaa caggtctgag tagtttttgc
    16861 acagtaagga gtaaaaacta tggttaaaga cccaataaaa aaagcaaaga atggaaccta
    16921 ttattttcgt gcaaatctag gttatgattc taaagggaaa aaaattcaaa aatatcgtag
    16981 tggattcact actaaaaaag aagcaagaga agaatattcg aaactgctat tgatgaaacc
    17041 agaagaatta agtgagaata aggataaaat gacgtttaaa cattatatct ttgaaatttt
    17101 ccttccatgg tataagacac aggtaaagct cagaacatat gaaaatcgct taccaactat
    17161 taagaagcat ttctcttatt ttgataaaat ggctgtttcc gatattgagc ctattgatgt
    17221 gcaaaattgg caactaaaat tatcaaagaa gtgtaagtct tcttatgtaa gagctgttca
    17281 aggactattt tcagttgcta tggatcgagc aattgtcctt gggataacca ccacaaatcc
    17341 atctaagatt attggcaatg tgaaaaagca aaagtctaaa attgaatttt ggacaaaaga
    17401 agaatttgaa aaagtaatct ctctaattta caaagaagat tactatcaac actttttatt
    17461 tatctctctg tggtttttat ttatgactgg aatgcgaatt ggtgaagcta ctgccattca
    17521 atgggaagat attgattttg actcaggagt actaaccatc aacaaaacac tctattacaa
    17581 gaatcaatcg aattatagat ttgttgaacc aaaaacaaag gcgagcgttc gtcatattgt
    17641 attagataaa tgcacattaa cttatttatc cgaatggaag gaagtacaac aaaatctaat
    17701 tcagacagac tttgtaatga gctataacgg tattcctacc caaaaacata cactatcata
    17761 tgctattgaa cgctatgcta agctggctgg tatccatcga atcagaatcc atgggttacg
    17821 gcattctcat gcttccttgt taatcagtat gggagaaaat ccacttatca ttaaagatcg
    17881 tctaggacac gaagatattg agacgacttt aggaacgtat ggacatcttt atcctaacag
    17941 taatttcgag gtagctaaca aattaaatgg agccatttcc tttaaagaag caaaacaaaa
    18001 ttatgatact tcaccaaaga atcaatttac tgtagactac ttgcgaaaca aagaaatgaa
    18061 aaaagtgcaa taaaagtgca gtaaaaagat aagaagagcc agaaaccctt ggtataagag
    18121 gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg
    18181 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
    18241 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
    18301 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
    18361 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
    18421 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
    18481 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
    18541 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
    18601 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
    18661 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
    18721 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
    18781 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
    18841 cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
    18901 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
    18961 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
    19021 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
    19081 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
    19141 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
    19201 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
    19261 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
    19321 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
    19381 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
    19441 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
    19501 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
    19561 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
    19621 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
    19681 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
    19741 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
    19801 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
    19861 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
    19921 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
    19981 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
    20041 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
    20101 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
    20161 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
    20221 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
    20281 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
    20341 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
    20401 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
    20461 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
    20521 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
    20581 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
    20641 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
    20701 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
    20761 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
    20821 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
    20881 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
    20941 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
    21001 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
    21061 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
    21121 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
    21181 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
    21241 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
    21301 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
    21361 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
    21421 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
    21481 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
    21541 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
    21601 ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca
    21661 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
    21721 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
    21781 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
    21841 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
    21901 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
    21961 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
    22021 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
    22081 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
    22141 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
    22201 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
    22261 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
    22321 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
    22381 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
    22441 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
    22501 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
    22561 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
    22621 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
    22681 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
    22741 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
    22801 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
    22861 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
    22921 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
    22981 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
    23041 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
    23101 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
    23161 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
    23221 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
    23281 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
    23341 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
    23401 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
    23461 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
    23521 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
    23581 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
    23641 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
    23701 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
    23761 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
    23821 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
    23881 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
    23941 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
    24001 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
    24061 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
    24121 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
    24181 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
    24241 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
    24301 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
    24361 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
    24421 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
    24481 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
    24541 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
    24601 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
    24661 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
    24721 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
    24781 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
    24841 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
    24901 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
    24961 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
    25021 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
    25081 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
    25141 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
    25201 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
    25261 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
    25321 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
    25381 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
    25441 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
    25501 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
    25561 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
    25621 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
    25681 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
    25741 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
    25801 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
    25861 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
    25921 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
    25981 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
    26041 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
    26101 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
    26161 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
    26221 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
    26281 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
    26341 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
    26401 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
    26461 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
    26521 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
    26581 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
    26641 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
    26701 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
    26761 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
    26821 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
    26881 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
    26941 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
    27001 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
    27061 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
    27121 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
    27181 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
    27241 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
    27301 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
    27361 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
    27421 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
    27481 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
    27541 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
    27601 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
    27661 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
    27721 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
    27781 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
    27841 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
    27901 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
    27961 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
    28021 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
    28081 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    28141 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    28201 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    28261 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    28321 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    28381 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    28441 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    28501 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    28561 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    28621 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    28681 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    28741 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    28801 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    28861 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    28921 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    28981 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    29041 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    29101 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    29161 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    29221 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    29281 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    29341 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    29401 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    29461 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    29521 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    29581 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    29641 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    29701 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    29761 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    29821 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    29881 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    29941 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    30001 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    30061 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    30121 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    30181 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    30241 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    30301 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    30361 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    30421 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    30481 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    30541 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    30601 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    30661 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    30721 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    30781 tgtgtttccg aacggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    30841 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    30901 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    30961 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    31021 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    31081 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    31141 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    31201 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    31261 cgttgaccag ctactgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    31321 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    31381 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    31441 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    31501 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    31561 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    31621 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    31681 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    31741 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    31801 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    31861 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    31921 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    31981 gggctaggag cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    32041 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    32101 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    32161 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    32221 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    32281 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    32341 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    32401 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    32461 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    32521 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    32581 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    32641 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    32701 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    32761 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    32821 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    32881 ttttcctcca attagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    32941 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    33001 gacagagaca ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    33061 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    33121 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    33181 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    33241 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    33301 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    33361 ttaactctga gtcacaagga acaatttcaa aaa
//
LOCUS       DAJSVF010000285        19509 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.050, whole genome shotgun sequence.
ACCESSION   DAJSVF010000285 DAJSVF010000000
VERSION     DAJSVF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.050"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(41..364)
                     /locus_tag="ON550_003137"
     CDS             complement(41..364)
                     /locus_tag="ON550_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3956123.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(351..1058)
                     /locus_tag="ON550_003138"
     CDS             complement(351..1058)
                     /locus_tag="ON550_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3956124.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            1144..1485
                     /locus_tag="ON550_003139"
     CDS             1144..1485
                     /locus_tag="ON550_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HCR3956125.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(1656..2504)
                     /locus_tag="ON550_003140"
     CDS             complement(1656..2504)
                     /locus_tag="ON550_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956126.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            3102..3965
                     /locus_tag="ON550_003141"
     CDS             3102..3965
                     /locus_tag="ON550_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3956127.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            4065..4547
                     /locus_tag="ON550_003142"
     CDS             4065..4547
                     /locus_tag="ON550_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3956128.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LSESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(4810..5277)
                     /locus_tag="ON550_003143"
     CDS             complement(4810..5277)
                     /locus_tag="ON550_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR3956129.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            5400..6251
                     /locus_tag="ON550_003144"
     CDS             5400..6251
                     /locus_tag="ON550_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3956130.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(6502..8460)
                     /locus_tag="ON550_003145"
     CDS             complement(6502..8460)
                     /locus_tag="ON550_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3956131.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSDKA"
     gene            complement(8457..9329)
                     /locus_tag="ON550_003146"
     CDS             complement(8457..9329)
                     /locus_tag="ON550_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3956132.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHTETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(9671..10492)
                     /locus_tag="ON550_003147"
     CDS             complement(9671..10492)
                     /locus_tag="ON550_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3956133.1"
                     /translation="MSRAAKSLHITQPTLSKQLKDLEEELGVTLFTRGSREIQLTDSG
                     RYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAISFISDSLHSLIKAHPDI
                     QFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLPAQDRWGLLIHRNHPLAK
                     QTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVGTYNLLYNASLMVDNGTF
                     GALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSPAARKFLDVLTEKYS"
     gene            10670..11239
                     /locus_tag="ON550_003148"
     CDS             10670..11239
                     /locus_tag="ON550_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3956134.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            11306..12043
                     /locus_tag="ON550_003149"
     CDS             11306..12043
                     /locus_tag="ON550_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3956135.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYSDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            12274..13542
                     /locus_tag="ON550_003150"
     CDS             12274..13542
                     /locus_tag="ON550_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3956136.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            14029..14493
                     /locus_tag="ON550_003151"
     CDS             14029..14493
                     /locus_tag="ON550_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3956137.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            14477..15106
                     /locus_tag="ON550_003152"
     CDS             14477..15106
                     /locus_tag="ON550_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3956138.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            15460..16938
                     /gene="msr(C)"
                     /locus_tag="ON550_003153"
     CDS             15460..16938
                     /gene="msr(C)"
                     /locus_tag="ON550_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3956139.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            16985..17431
                     /locus_tag="ON550_003154"
     CDS             16985..17431
                     /locus_tag="ON550_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3956140.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(17630..18145)
                     /locus_tag="ON550_003155"
     CDS             complement(17630..18145)
                     /locus_tag="ON550_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3956141.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            18262..19463
                     /locus_tag="ON550_003156"
                     /pseudo
     CDS             18262..19463
                     /locus_tag="ON550_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6175 a         3475 c         4007 g         5852 t
ORIGIN      
        1 atgccttttt atttttctaa cttaatttta caattcgcgt ttataataag cgaatcaacg
       61 aaagggaaag aattcctgcc aagacgatca ccaataagct ttttgaaaga atagcagcta
      121 aaagtgtagg aactgaagcc aaagcatttt cgagattgat ctgcggcaga tgtccgatat
      181 tttgggtaaa tagactgctg aaccataaag cagacataat gacgatcggg acaaaactta
      241 aatactcaag caacggttgc ggtaaatcga attttttcag taagacaaat ggtaggaccc
      301 tagaaagcca agttgctaaa gtacaaccta aaatagtcag caatgtaaac tcaaaagaag
      361 gcatgtttga tccacacccc caatccgcac ccaatcagtg ttacaactac aatcactaag
      421 ttgcttggga taaaaatcag tccgacatat accagaatca aagtgagacc aatcaggatc
      481 aactgtaatc gtttagaggt gtttctatca gaaatgactt gaagataaag taacccgata
      541 aacatagcga cgatggcgaa atccaaacca aactttttcg gatcagtaat aaaattccct
      601 aaaagtgcgc caacaagaga agaacccacc cacgtcaaat aagaaatcca attagctgta
      661 ttcatccaac gaaagtttaa cttttgtcca gtatagttca atttattcat tcccagtgcg
      721 aaactttcat ctgtcagcaa tgtgccaatc agcagatttt gaagcagccg atttttcttg
      781 aaataaggag caatcgtcat actcatcaga atcattcggg aattgactag aaaggtcgag
      841 aaaacgatgg aaagcaatgg gctgtgacta gcaagcatgc tgactgtgat aaattgggca
      901 gaaccagcat agaccagcag ggacatcaac gtgacgacta gtggatgaaa tccggcagct
      961 ttcccaacga taccaaatgc aagtccaata ccgatataac cgaaaacggt aggtaacgtg
     1021 tctttgatcg cagtttttat atctaacttt tcatccaata acaaaactcc tttagcacga
     1081 cataaatttt cataattgtc gttaaacttg tgaatattct aaatttttaa tgagcatttg
     1141 tcaatggtgg gattgaaacg taaagaacta gcgggtcgca gatttacacg tattgtttcg
     1201 gtgggaggag gcgcgaaaaa tcttgcatgg ctacaaatcc aagctgatat tttcgatgca
     1261 acggtctatt gtttaagtgt ggaggaagga cctgcattag gcgcaacaat gattgctgca
     1321 ctagggttag ggtggttcga cacacctgaa agttgttgtc agaattttat ccaatataca
     1381 gatgcagtgg aacctctctt acaaaatgta gaaaagtatc ataaaatcta tcaacaatgc
     1441 cggaaaatct atccagcaac gaaggaaatt tgtcatgact tataaaacaa aacgcgtacc
     1501 taatagtcaa atgactaata ttgggtacgc gttaagtctt ctttacttca tcctttgcat
     1561 gattcgtgtt tttaaagcaa ggtctgctgt atttcttgtg gatttaggat ggttgttatc
     1621 gtttacttgc tatttttcat tgaaatcgtc tttctttacc gtttctttag ttttttatct
     1681 atttttttct tttggttaga aaattctttt tcccgaaagt cttctttcag gatttttatg
     1741 gagtagcgtt ttgaataaga ggcgtcagta tgatcgactt ggcagacgaa ccaagagaac
     1801 atttgccttt tttctgcatg aggtgcttct gctaataatt ctttccataa ataataacag
     1861 tcctctatca gaagaagggt tcgttcatcc tcaggctccc aatagaatgt ttcgagagta
     1921 gcaatcactt cattgatcaa ttcgaatgcg ggaagatagg cattttgctg aataagcgat
     1981 tcaactgctt cttggatgaa ctttcgcatc tccatgatga acttcgctgt tttcctgttt
     2041 ttgtttttct tgataatatg atcgtattta tggatgatag ttgttaattt atctgaataa
     2101 tttattgcat gaaatggcat cttaactttg aagcgttcga taaagtgcgg atgttctata
     2161 agaatctctg ttaaaagctc ccgtacaaga gaggaactag cggaggaaac aagggacgag
     2221 agttcggaaa gtgagtagtt ggattttttt attggacgtt cttcgtaagc gaataagaca
     2281 gcagccatgt gtttgcattt ttttccttct atggcatata gacaatcaca agtcatgttg
     2341 gtcttttcat cccaagtgat cactacctta taatcttccg tgccagaaac tgttgcggac
     2401 aagtacttgc ttgtatagtt caaatgacgg acaaaaccgt gcagatgata gctgtaaccg
     2461 cgttccaaaa cgttttcagg aaaatactca agccaattat tcatctagtt ctcctttcat
     2521 gaaacactta tttatattta gtataaagaa acgtttaaga aaaaagtagc tgactttaca
     2581 tatttgataa tcacttttgc tcgaattgtc aaaaattcat tctccttttc ttttcttaga
     2641 gaatgtgaga aaaagtaaaa gaatagcgtg ttttaaagat ggaacttttt ttgtttttta
     2701 tttatctgac aaaaatgaag tgattttgtt agaaataaat aattgttcca attgataaaa
     2761 gaggtatgat gtatcgcaaa gaaaaatcca agcagcgttc tgatcagcag tttgaacaac
     2821 aatttgcttt gtacagtagg tagtaagcgg attaactaag ggaataaaag atatttttac
     2881 cgctttgttt acttagaatc tgtcttcccc aaaatttatt tttctaaagg taaaacatag
     2941 ataggcagat aataccaatt atccgttacg aaacgctgct tgagaatttt ctttagatcc
     3001 atacaacatt ttctacatct gactaaattg gttcctgatt catcaagtaa ttgactttct
     3061 ttgataatat gataaaaagg caagcaagga ggaagataaa tatgcttaaa gatctgaacc
     3121 aattgatcga ttacatcgaa gaacatttga cagaaggact ttcctataaa ataatagccg
     3181 aaaatacagg aatctctgaa tatcaattaa aacgcatctt tacgtttatt gcagggatgt
     3241 cgctaatgga ctatatcaaa aaccgtagat tggctttggc taatcgcgat cttctagatg
     3301 gatcaagagt gacagaaacg gcgtttagat atggatatga aacagtcgaa ggtttttcaa
     3361 gagcttttcg tgattggtca ggatatctgc cttcagaaat cagcagatac ggcatacaaa
     3421 aatcttttcc acgcttgaca ttttttattg atgtcagagg aggaaaatcg atggaatttc
     3481 gtatcgaaaa gaaagaacag ttttatgtgg caggtgtggc gaaaaaagtt cctgtcaaat
     3541 ttgaaggagt caatcaagaa atacaaaagt tagcgcagac gatcacggag actcaaaggg
     3601 aagagatgca taagttagga gatttatatc caaatcaagt aatcaacgtt tcttatggat
     3661 ttgatggaga ccgcttggaa gaaaagggca gtttgatgca catgattggc tttgctacga
     3721 caaaagaaaa tccgtacgct gatttagaaa tgcttgctat agaagaacac acttgggcca
     3781 tttttcctaa taaagggcca tttccccaaa ctttacaaga tacttggggg aaaatctatg
     3841 cagaatggct tccttcttca gattatgagt tagtcgaagc tcccgagatc tcttttacaa
     3901 aatggggaga ggatttcagt aatgtttaca gtgagatttg gattgcagtg aaagcaaaaa
     3961 aatagttaaa aaataaaaat atccctagaa aaaagagcgt aatcgtgtca tttcccttta
     4021 attttatgta agatgaactt aatataatag aaagggtggg agaaatggca aagtatagga
     4081 tcgaagaaaa accagatttt acggtgacgg gtattggtgt ggaattgaag agtagcaata
     4141 cggattttgt cggtttggct gcagagaaac aagcatttga gcaaaaagtt atcggtaatg
     4201 gaacaataga tgcattgaaa caagctgctg aaaatcatta tttctttaca gtcaacgaac
     4261 cctataacgg gaaaatgatg tactatgtcg gtgttcaaac gagtcaggat gttccaaaca
     4321 ctacgcgaat catcccattc ccaaaaggaa cgtatcttgt tgtaagcgct ttatcagaat
     4381 cgcctgaaga attaagaaat gatttgacga atacggcctt tgggcatgtt ttaagagaag
     4441 tagaagatta cgcctatgtt gggggtccaa atgctgtagt cgaaatggga gttaccaacg
     4501 ggaaatatat gggagaaatc tggataccgg tagtcaataa accatgataa tatagaggtc
     4561 gtgaaagtag cgctttgacc tgagcattag agaggaaaaa tgggaaatag ctttccatat
     4621 tttgcatttt tcagaactaa tgccgagggt cagctacttg aacaccgttt atcaagacgt
     4681 catgacttca gcgctttgac ctgagcatta gagaggaaaa aggggcatga caaggctttt
     4741 gtcccccgga gaataaaaga atttatgtag acgattccga tcagaaaaat gcaggcacag
     4801 tagagagaat tataaaaatc tctctacgag gcctgtgttt tctggtcttt tttctttttc
     4861 caaaacggct attttatgta tcagactatc aagtgtgatc ttagccattt cttcttctgc
     4921 ctttttctgc agccgagcgt agctttcttc taaagcttct tggatattcg caccaacgac
     4981 gcaatttgga tttgtttttg gatcgacttg gataagctgt gtattacctt cgatgctgcg
     5041 atagacatct agtaaagaaa tctgatctgg acttttggca agggaaggct gcggttttcc
     5101 ggtttttgta aagatcaaac cggatttttt gagctgactc atgattttac gtacatttgc
     5161 tgcatttgtt tcgatactac tggcaatcat ctcactagaa agtgatcggg tatcttgaaa
     5221 catttcaata taggcaagta catggattgc atcactaaat tgcagagaat acttcatcag
     5281 ttgtcatcct ttcatttttt ttgcttgact tatcttacca cactctttat gataaaggtg
     5341 taatttataa atgtacagct gtaaatctta acaaataaaa agaataggga gtggcaaata
     5401 tgcattattt agtaacagga gcaactggag gttttggtgg ttatgcgctg gaattcttaa
     5461 aaaagatggt tccacaaaat gaaatctatg tactagtacg tagtgaagaa aaaggagaag
     5521 cactgaaaga agctggattt aatatacgta ttggtgatta tgatgattta gactcaatga
     5581 aagaagcgtt gaaaggaata gatcgattgc tttttgtctc tggcgttcca ggaaatcgtc
     5641 aagccgaaca taagaatgta gtgaaggcag cacaagcagc tggggtatct tatatcgctt
     5701 atacaagttt tgcagatgcc gatcactcga tcagtgtatt agcgccagat caccaattca
     5761 ctgaaaaaat gattaaggat tcaggtattt cccatacatt tttacggaat aattggtatg
     5821 tagagaatga attacctatc attgggcagg ctttaaaaac tggtcaattc gtttatgctg
     5881 caggaaatgg aaaaacaggt tgggcattga aacgtgaata tgcagaagct gcagcaaaag
     5941 tactagtaga aagtgactct ccagaaatct tagaattatc cggcaatcta actgactatg
     6001 aagaattagc caaggcatta gagcgtgcga caggtaaaga gttagaaatt attgaagcaa
     6061 gtgatgcagc gtttgtcgaa aatctaaaag aagccggatt ccctcaagaa gcagctgaca
     6121 tgtttttagc gatccaacat gatattaaaa aggatcagtt agctatttcc tctgatgatt
     6181 tagaaaaagt attgggaaaa ccattaactt ctcttgaagc agcattaaaa gaaatattgg
     6241 atcaggaata atgtataaag aggttgtgaa aaagccgatt agttttgagt atgaagaaaa
     6301 attttgtgaa aaagctgttc tgcatcaagt aataagaaga gtcaaataaa aaatagctct
     6361 tcatatgttt cgtttggctg gacttattac cgcggagttg gcaggagaac gccgttttta
     6421 ttcggaaaaa ggagctgaga caagattttt gtctcagctc ctgtttgttt atcgataaaa
     6481 aagtctgggg aagacgatgg ctcaggcttt gtcggaaaca gagccgattt tatcatttgc
     6541 ttttacatca cgtgcgggtt cgatttctaa gctttggact ttgtcgccat ttgtgaatac
     6601 aacgaccata tccgtgtttt tgcctgcaga ttgaatagcg gcaagatcaa ctaaagcaat
     6661 caattgacct cttgcgactt tttgaccttc ttctacatat aaagtgaatg gttcgccttt
     6721 taagtcaaca gtgttgatgc ccatatgaag caagacttcg ataccagcat ctgtttggat
     6781 tccgacagca tgtttcgttg ggaaaatgtt tgtgatcgta ccagcgattg gagagaagat
     6841 ttctccattt tctggtaata cagcatagcc gtcacccatc attttttccg cgaatacatc
     6901 atctgaaact tcagtgattg gaatcagttt cccatttgcg atagcgtaaa tatcttgttc
     6961 gatcgtttca ccggcaaatg tttctgtttt aaccacggtg tttgtttgtg caggtcgtgc
     7021 tgcagcacca ccatgtagtt ttgccatctc atctgcaacg aattgtactt ctgtacctac
     7081 gatgacttgc gcattttttc cgtcgatgat cttcatacca ggtacgccag tggctttgat
     7141 tttattttga tcgatcaggc ctgtatcttt gacttgcaaa cgaaggcgtg tagtacagtt
     7201 atcgatcgaa gttacattgt cggctccgcc taatgcagcg tagattttgc ttgcttgtgc
     7261 cgcatattta ttgtctgaat tagtcgttgc agtatcgtca tctgaaagat ctgcttcctc
     7321 gcggccagga gtcatcagat ggaatttttt gatagcaaag ttaaagccaa agtagtaaat
     7381 cactgctgtt actaatcctt ggacgatcaa catcaatggc tgattcgcaa tcggattttt
     7441 caaagatagg aagtaatcga caaatccggc actaaaggca aatcctgctg tccaatggaa
     7501 gaatgcactc actgctaaag aaatcccagt gaaaattgca tgcaagacat ataatggcca
     7561 agcaacgaac ataaatgaaa attcaagtgg ttctgtcaca ccagtaaaga atgaagcaaa
     7621 agctgctgct agcatcaagg aagctgtttt tgctttgttt tctggacgtg catttctgta
     7681 aatagcatat gctcctgctg gaagcccgaa catcatgatt gggaaaaatc cagcttgata
     7741 cattcctgtt acgccttttg ttccttcaga agccaaaaat ttaccaatat cattgattcc
     7801 tgctacgtca aaccagaaca cggagttcat tgcttgatgc aatcctgtag ggatcagtaa
     7861 acggttgaag aagccataaa gacctgcacc accccagcct aaactcaaaa tgaatttagc
     7921 aaaagaaact aaagcagagt aaaccggagg ccatacgagc agtagcactg cactgatcag
     7981 caacatgaca aaggcagcca agattggcac tagacgcttg ccgctaaaga aagataaagc
     8041 catcggtaat tttgcttcgt ggaaacgatt ataaagagct gccgcaatca atccggcaat
     8101 aataccaata aatacgttac tgatctgagc aaaagatgga tcaactgatt ctggtttgat
     8161 attcaataaa gtagctactg aatcagtttt taaaacattt actggaactt caaatgcgac
     8221 caaacctgcc aaggcagcag cacctgattt gtccactgac atccctagtg ctaacccaac
     8281 ggcaaacagc aaaggtaatt gacctaaaac tgcgttaccg ccagcttgta aaaaatgagc
     8341 gacaatgtcg ttactataag atgcccacca gtttccgatc cctactagta aagcagcagc
     8401 aggtaagacc gcaactggaa gttgaaggga acgtcccatt cgttgtagat atgctttcaa
     8461 tttttttcct cctcatgatt gttaacattt gtttgtacga ttgcgcttct taaacgctcg
     8521 atatggatgg ccaagtaacc cagttcttct ttcgtgatcg cgagctggta ttctttggtc
     8581 agcaacacac ggattttttt agaaatctta tagctttctg ggtattttcg tttgaccatt
     8641 tctacgattt ccgtgtctaa aacagcatat tgttgcgcat gggttcgttg tagaagcagt
     8701 cgtaaatgat tcaccagtct tgaataattc agtgaaaatg gttctgaatg gatatctgtt
     8761 tccaattctg attccactaa atggatgatg tcagatacaa tcgtcacttc acggatactg
     8821 cgatggttat tgttccgacc gcttcgtccg ctgtgaatat ggattgcgat atatcctgct
     8881 tcgtcataag tatatggaat cccaagctct tttgtcagat actctactgc ccattgagca
     8941 atactgaatt catcactgta aagcacttcg atttctctta aaagtttgtt gcggatgacg
     9001 ataccgttat taatgttctc cgcagaaaaa gcaatgtggt cggtcaaagc gatcaataca
     9061 tgttcattca acttttccat caaaacagtt tccgtatgat caatgatcgt ttctgctgca
     9121 aataaaaagc gttcatcaat ctgattaagt aaattttgca acttgatttg accttctggt
     9181 tccatgacaa atatccgttc gacatcacga tcaaaaatca aatcatttct ttttctgtca
     9241 aatccaatcc cttttccaat agctactttt tcctgtccac cgtcatctac caatactgca
     9301 ttttgattaa agatcttttt gattttcatg cgcgcaccct ttttgaagat ttggttttca
     9361 cgtttgctag accagttgat tgcggccaaa caaaaataga cacgaaaaac ctcctaggga
     9421 aaccatgttc ctcagagatt tttcatgcct agtatgatct agtcacatga aacgaattat
     9481 tgttaacgta atcaatttta tatagacagt tatgttttgt caattctttt tttataatta
     9541 tagtaattta ctggaattat cagaaataaa aggaagaaat aaattaagaa acttatgtta
     9601 tttaatcgtt ggatcttttg cttatcttat tcgttgttaa aaagctgaaa gcgaaggaaa
     9661 aaaactctac ttaactgtat ttctccgtta ggacatctaa aaatttacga gcagcaggag
     9721 aaagaggctg gtcttttttc caaatgagat tcagtgaagc ttgtaatggc ggatcgagtg
     9781 ggatgaatat tttttccgtc ccataagtat tgatgatccc atctagagct aatgcaccaa
     9841 aagtcccgtt atcgaccatc aaagaagcat tgtacaataa gttatatgtt ccgacgatcg
     9901 ataactgatc tatgctgaaa cctagccatt cagctagttg actgtccact gacgtttgcc
     9961 gggaaatgat caaaggaaca gttttcagtt cctgcggatg gatgacggtt tgctttgcta
    10021 aaggatgatt tcgatgtatc aaaagtcccc aacgatcttg ggctggcagt cgaaatgact
    10081 ggtatttttg tttgtttgtc ggttcgatta cgagtccaaa atcaagtaat ccgctatcca
    10141 gtttgtctat gatatcatct gcattcccgc tatataaatg aaactggata tcaggatgcg
    10201 ccttgatcaa cgaatgaagg ctatcagaaa tgaaagagat tgctttagtt tcgcctccgc
    10261 caatgtagat ttctccagag atgattgcgt catttgttaa attgttcgcc gttttttcta
    10321 ccaacgacaa gatttctttt ccttttgaat aaagatatcg accagaatcc gtcaactgta
    10381 tctctcggga gccccgagtg aaaagtgtca caccaagttc ttcttctaaa tctttcagtt
    10441 gttttgagag agtaggttgc gtgatatgca gagattttgc tgctcggctg atattttttt
    10501 ttctagctac tgctaaaaat acgtcagtat acgaatatcc attttttccc tcctgttttt
    10561 aaaaaagcat accactaagt atagttaaaa ggaatagtta agtatgtttt ataagtatta
    10621 gacatgataa gccgtccgtt ataaagtaag gataaaagga gggagcaaaa tgacaagcct
    10681 catcgaaaaa caaatcgtca ataaagagat tcgagtaacg gatccgctct ttgaagtgat
    10741 ccatcagatc caagctgaaa acgaagagcc tttagcagcg ctgaatactg ggtaccatga
    10801 accagcagat atccgcaaac ggctagaaaa tatcattagt gacaaagtag atgatacggt
    10861 gactgttctg ctgccttttt atacagattt cggcaaacat attagtatag ggaaaaatgt
    10921 ctttatcaat cgtcaagtga tgttcgttga cttaggtggc atctgtttag aagattcagt
    10981 attgattggt cctcgtgtca acctcatcac ggtgaaccat ctaaccgatc caaaagaacg
    11041 aagaggcttg tcagtaaagc caatccacat caagaaaaat gcttggatcg gagcaggagc
    11101 aactattctg ccaggcgtaa cgattggcga gaatgccatt gttgcagcga atgcaactgt
    11161 tacgaaagat gtcccagaca ataccatcgt tgcaggaatc cctgccaaaa tcgtcaaacc
    11221 tgtcgaacgg attatttgaa cagtaagatg aaataagaga tagtgagtat aagcaagaga
    11281 tttttcaaaa caggaggaga atagaatgaa tgatttatca gaaaaagtga ttgtcatcat
    11341 gggcgcttca agcggaatcg gtgaagcaac aacgaagcta ttagcagaaa aaggggctaa
    11401 actagttata gctgcacgcc gagaagatcg cctaaaagca ataaaagaat ctttaccaga
    11461 agctgagcta tatattcaaa cagcagatgt aagagatttt gcacaagtcc aagcagttat
    11521 cgatttagcg atggaaaagt tcggtcgaat cgatgtattg tacaataatg caggaatcat
    11581 gccaaccgct ccattagttg aaggtcaccg tgatgaatgg caaaatatgc ttgatatcaa
    11641 catcatgggt gtgttaaatg gaatttccgc cgtacttcca atcatggaaa aacaaaaatc
    11701 tgggcatatc atttcaactg attctgtggc gggacatgtt gtttattctg attccgccgt
    11761 ttattgcgga acaaaatttg ctgtccgtgc aatcatggaa ggattacgtc aagagcaacg
    11821 gcaaaataac atcaaatcta cgatcatctc tccaggagct gttcaaactg aattatatca
    11881 aaccatcagc gacaaaaagg ctgctcttga acttcatgaa gcgcaaaaag aatggggatt
    11941 gacctccgaa gatatagctt ctgctgtagc atttgcgata gaaacgccag accggatgtc
    12001 tgtcagtaac atgattatcc gtccgacgac acaggaagta taatccataa cggataatat
    12061 ccaaagagaa ttacttgtta aatagttatt caaacgacca tcaatttaag gcgtgaatgg
    12121 agaagaatat tcttcattta cgtcttttta tgtagaaaaa gcaaatttat caaaaagaaa
    12181 caaatgttat ttaaatatgg acattgactt gcttttactt ttttagttac actgttattt
    12241 aggaaaagtt tcataattca aaggagaaaa aagatgaaag caaagaattt attatttagc
    12301 gttattcttt taggaaatct agcagttgcc aacacaacag tgctagctga agaatcgaca
    12361 gaaactgtcg caactcagac atcgagttct tcagaaacag gaattgaaaa cgatacagag
    12421 agtactttgc agtcagctac tgtctcagaa aatacaattt caaccgaaga accaacaact
    12481 gaaacaacag aacaaacaca aaccgaagac agtacatcag cagaagctca gaacaatcca
    12541 gagaatgcag aagaaatcac gaaccaattg acagatgggc aaacattgac acatacccat
    12601 caagcagatt tgtctgaatt cgaaaactat actggctatg ctttttatgc tcgctcatct
    12661 gctaattctc aacaagcgtt cattgatagt attgcctcaa ctgcacaaag tttggcaagt
    12721 gccaatgact tatatgcatc agtcatgatt gctcaagcca tcgttgaaag tggctggggg
    12781 aacagtactt tggcttctgc ccctaattac aatctatttg ggattaaagg aagctataat
    12841 ggccaatctg ttacgatgcc tacttcggaa tatgtgaatg ggcaatggat tacggtgaat
    12901 gccgctttta gaaaatatcc ttcgtacaaa gaatcattgc aagataacgt aacggtattg
    12961 aagacgactt cttttcagcc aggcgtttac tactattccg gtgcatggaa aagcaatacg
    13021 aactcttaca aagatgctac ggcttggcta acaggtcgtt atgccactgc gcctaattat
    13081 ggttctacat tgaacaatgt aatcgaaacc tataatctga cacagtatga tacagcacca
    13141 acttcaactt ctccgatgta tcgtctgtat aaccgtcata caggagaaca tttatataca
    13201 ttgaatgccg gagaaaaaaa ttatctgcca actgtaggct gggaatatga agggattgct
    13261 tggcaagcac caaacagcgg acaacctgtc taccgtttat acaatcctta cagtggagac
    13321 catcattaca caatggcaca atctgagatc aactctttaa caaaaatcgg ttggcgttat
    13381 gaaggtctaa gtttttattc tggtggttcc aagccaattt atcgactatt taatccaaat
    13441 gaaaaaactg gtacccatca ctataccttg agtgctaagg aaagagattg gctaacaccg
    13501 atgggctgga gatatgaagg aataggattt tatggttatt aaaaagaggt agtgaaaaag
    13561 ctgccctgca tcaagtagta agaacagcca aacaaaaaca gattttcatg ttttttgttt
    13621 ggttgaactt attaccgcag atgcaagttt ttgaacaccg tttactcaaa aaaggttgtg
    13681 acaacatctt gtcataactt ttttattact gtttaagcat actgatccaa tttctaatat
    13741 tcgacgggat tcttgcaaaa ggcgatttcg gaaatttttg gcggtgtaaa attttctact
    13801 gcagaagagc tatcttctat agtttgataa gaatcaataa tcttggtttt agcgttgtgc
    13861 tttctttatc attttttccg ttcattctac aaaaaatttc ttttctctat acttttctat
    13921 tgagagcgtt ttttttatta cacagctgat tctttgactt ttttcttttt ttattctatt
    13981 attgacaagt caatcgttga cgagtcaatt attagaaagg aggaaattat gtctttagaa
    14041 gagattagtc accttttata tcaaatcaaa ttagccgatc aaaaaatcgt ccagatcttt
    14101 gaagaaaata tcggtataag tcttactaga tacgagatca tgatgagact gaaagaagaa
    14161 aatccattgc tacaaagcag tttgcaagaa aagttgcaga ttgatcaagg agcagttact
    14221 cgtcatctga aggtattgga agcaaatggc tatgtttcgc gtcagagaaa tcctcagaac
    14281 aaccgggaag tgtatgtagc gctaacagaa aaagcccaga agaaaatcga gcattgtgaa
    14341 atggaccata aagatacagg ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa
    14401 caatttctaa cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg
    14461 aaagaggtta ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag
    14521 atccgttcgc gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat
    14581 caaagaagcg acgactgcac cttcttctgt caatatgcaa ccatggcgct ttgtggtcgc
    14641 tgaaagcgag gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa
    14701 tgatacttct tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga
    14761 agaaatctat aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca
    14821 attagctgcg atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt
    14881 caaagtcgat tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta
    14941 tgaaacaaat ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga
    15001 taaagaccgt tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta
    15061 cgagtctgtt cgtttagcac cagaaaagat cactacgttc gaataaaagg ttttcagtta
    15121 ctattcagta gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc
    15181 ggaaaaatga ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat
    15241 tcaaaagaca tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac
    15301 agtagaatag gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga
    15361 gtaggaaaag atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc
    15421 gataattgtt gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat
    15481 ataacaaatc tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt
    15541 gtctatcagc aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg
    15601 ctcaaactaa tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc
    15661 ttcaactacc tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta
    15721 gccagtcatt tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa
    15781 gaacgaaaga tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac
    15841 gagccaacaa cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat
    15901 tattatggta cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg
    15961 aaaatctggg aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat
    16021 ctcactcaaa aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa
    16081 gagaaaaaac gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa
    16141 aaagtgtcca gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc ttcctctaaa
    16201 caaaaagaca gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc
    16261 caaaaaatag gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca
    16321 aaatctcttg aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga
    16381 agtgggagaa cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg
    16441 attgtcggtg aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga
    16501 gaaggaatcc ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa
    16561 atgacatctg aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg
    16621 cttgtccgta gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta
    16681 tgtacattaa gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca
    16741 agtaatgtgt tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct
    16801 ttagagaagc taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac
    16861 tttgtcgagc gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta
    16921 cttacgagaa atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta
    16981 cagattgcct acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat
    17041 ggtcttgggg atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt
    17101 gttgattggt ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg
    17161 tgtgctggcg aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat
    17221 gatcttaact atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg
    17281 tgtgatcatg gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa
    17341 aatgaatgtg attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag
    17401 tgtgctaaaa gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt
    17461 ggcatgcttt ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat
    17521 aggttttggc ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga
    17581 agataatttg ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa
    17641 actgctgcat ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa
    17701 ttctgcgctg gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat
    17761 tcaatttttt ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca
    17821 gtccagtagc tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat
    17881 ctttttgcgt aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt
    17941 catcagtcaa gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat
    18001 aaccctgctg gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa
    18061 aaattgtgat cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata
    18121 ataatatata ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat
    18181 atcacagtaa agtttccacg ttttacatgt gcatatttac atgtaaaata aatcatgtat
    18241 gatgctaata gagagaaagg gatggaaata ttggagaatc aaatagatat acaagaacaa
    18301 acaaatgaag aaacaaaggg gaaaagttca gcgctagttg tagcagctac ctgtatgatc
    18361 gcttttatgg gagtgggtct agtagaccca atcttaaaaa cgattgctgc acagttaaac
    18421 gctacgccag ctgaaacgac cctgttattt actagttaca tgttagtcac tggtgtggtg
    18481 atgctattta caggtttctt atctagtcgg atcggtttga aaaaacattg atgacaggat
    18541 tgtttatcat cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga
    18601 ttgggttacg tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa
    18661 cgatcgtcag tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac
    18721 ttggctgcgg aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc
    18781 gttatccatt ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac
    18841 ttttacctga aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt
    18901 taagcaatca taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct
    18961 ttactttact tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt
    19021 ttgtattttt tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc
    19081 ttgagcggaa gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt
    19141 gtttgctgtt attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt
    19201 tagcaggatt tttccaagga attatcaata cactgttgac gacgattgct atggaaatac
    19261 caggtctgca acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag
    19321 ctttagcacc atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt
    19381 attttgcagg tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt
    19441 ttgctacaaa tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag
    19501 tcataagaa
//
LOCUS       DAJSVF010000286        16229 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.058, whole genome shotgun sequence.
ACCESSION   DAJSVF010000286 DAJSVF010000000
VERSION     DAJSVF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.058"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(134..577)
                     /locus_tag="ON550_003157"
     CDS             complement(134..577)
                     /locus_tag="ON550_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3956142.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ON550_003158"
     CDS             complement(795..2135)
                     /locus_tag="ON550_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3956143.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ON550_003159"
     CDS             2404..3300
                     /locus_tag="ON550_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3956144.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ON550_003160"
     CDS             complement(3450..4391)
                     /locus_tag="ON550_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3956145.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHADLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ON550_003161"
     CDS             4517..5638
                     /locus_tag="ON550_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3956146.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON550_003162"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON550_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3956147.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="ON550_003163"
     CDS             7426..7992
                     /locus_tag="ON550_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3956148.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ON550_003164"
     CDS             8203..9264
                     /locus_tag="ON550_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3956149.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ON550_003165"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ON550_003165"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3956150.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ON550_003166"
     CDS             10177..12159
                     /locus_tag="ON550_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3956151.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ON550_003167"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ON550_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3956152.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ON550_003168"
     CDS             12881..14305
                     /locus_tag="ON550_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3956153.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ON550_003169"
     CDS             complement(14486..15259)
                     /locus_tag="ON550_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3956154.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ON550_003170"
     CDS             complement(15256..16161)
                     /locus_tag="ON550_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3956155.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
BASE COUNT         5210 a         2951 c         3377 g         4691 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 taatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccgcgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcat
//
LOCUS       DAJSVF010000287         7225 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.116, whole genome shotgun sequence.
ACCESSION   DAJSVF010000287 DAJSVF010000000
VERSION     DAJSVF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.116"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(472..645)
                     /locus_tag="ON550_003171"
     CDS             complement(472..645)
                     /locus_tag="ON550_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956156.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            1062..1628
                     /gene="amaP"
                     /locus_tag="ON550_003172"
     CDS             1062..1628
                     /gene="amaP"
                     /locus_tag="ON550_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3956157.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            1641..1829
                     /locus_tag="ON550_003173"
     CDS             1641..1829
                     /locus_tag="ON550_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3956158.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1842..2399
                     /locus_tag="ON550_003174"
     CDS             1842..2399
                     /locus_tag="ON550_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3956159.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            2431..2673
                     /locus_tag="ON550_003175"
     CDS             2431..2673
                     /locus_tag="ON550_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3956160.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            3014..4228
                     /locus_tag="ON550_003176"
     CDS             3014..4228
                     /locus_tag="ON550_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3956161.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            4259..4477
                     /locus_tag="ON550_003177"
                     /pseudo
     CDS             4259..4477
                     /locus_tag="ON550_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4896..5369
                     /gene="dfrF"
                     /locus_tag="ON550_003178"
     CDS             4896..5369
                     /gene="dfrF"
                     /locus_tag="ON550_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCR3956162.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            5496..6152
                     /locus_tag="ON550_003179"
     CDS             5496..6152
                     /locus_tag="ON550_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3956163.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            6334..6864
                     /locus_tag="ON550_003180"
     CDS             6334..6864
                     /locus_tag="ON550_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3956164.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         2517 a         1057 c         1479 g         2172 t
ORIGIN      
        1 ccagttattt tagattctat gtgtgtctct tctataatgc tagtttagat gcctgggatt
       61 atattggaca atacagttgg aggatttttt attatctagc gaagagaagt gtaagttaac
      121 agaactgtat gagaaaaaag acaactacgt agtagctaac cgattcatta attttttcaa
      181 gttcatgttt atttacgtat cagaattttt cttattcaat ggtttggtgt aatcaccact
      241 tatttgtcct ttattgctta tacaatgtta tacaaaagga aaatcaagca ttgacgcatg
      301 gggtggtact atctagctga aatagcttaa taagcgcaag tagatgcatt ggattagttt
      361 ataaatcaaa agtgttagag gtattaagaa gaagtagaaa aataaaatgg attgccgaat
      421 aaaaagtacc ataaatccta aaacatgtgc caacagatag atggcacatg tttatttttt
      481 tcgataattt tgtaacaaaa atacgaagat gaaaaagaat agagattccc ataatgtagt
      541 agtccaactg aattctttac tgataccaaa taccatattt cctagatttg atacaatcaa
      601 atagccaatc gttatgatca atgaatagta caatcttttc atcataggac atacctcgtt
      661 tttatttata agaatagtat aaaccaaaga agtgaataag aaaaatgata atataaaaat
      721 aaagtaagaa cgcattaaag taactaaagt tatttattgt attttttaat tctccgaaaa
      781 aatttaataa ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa
      841 tcttgaataa tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc
      901 aattgttatt tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt
      961 agcgctttat ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag
     1021 agttatttac agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa
     1081 taatcggtgt tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca
     1141 atgcgccgta tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt
     1201 actacatgca acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca
     1261 tcttgctagt cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag
     1321 atggcaaact aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg
     1381 aagaagagtt cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata
     1441 tccatgtcaa cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga
     1501 tgcaagatat cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg
     1561 cagtcacata cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag
     1621 tggaatagga gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct
     1681 ttgggggtct tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat
     1741 tgttgatcat aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag
     1801 gaatagttga tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga
     1861 atacaacaaa tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg
     1921 aattgactta tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag
     1981 atggtctatt gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca
     2041 cagataacgt aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc
     2101 tagatgtgat cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat
     2161 tgatcagtac tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg
     2221 ttgcagacat caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag
     2281 taacagaagc agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat
     2341 ctgctgtagg caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag
     2401 aagtaagaag tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc
     2461 ggtatcattg gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc
     2521 gcaaacatca ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg
     2581 ggaccaacaa ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta
     2641 ttagtcgttt cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa
     2701 atgaagaaca aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta
     2761 tatgaagtat tctttattcc ttatgaaaaa atatctttaa aaataaaaaa agacgaaaat
     2821 aaacggtttt ctataaaaag aagaccgttt attttttgtc tttatttgtg tgaaaaacga
     2881 aaaagagcac aacacatcaa gcttttatta attgtctgtc ttttataaat gcaagtatta
     2941 attcaaaaaa acagaagaaa aaaacaacta caccttcttt tatactcgta atcgtagaaa
     3001 ggaggcgaaa aaaatgaata cattacctat caatacagga gatacagcat tcatgatttt
     3061 atgtacagca atggtttgcc tgatgactcc tggtctagct tttttttacg gcggattagc
     3121 acggaagaat aatatactaa cgatcatgtc tcaatctttt ctctccgttg ggataacaac
     3181 gattatgtgg gtatttggtg gattcggatt ggcctttgga cgggatatcg gcggtgttat
     3241 tggaaacccg gcagatttct ttttgatgag acatgttaca agcttcccca acatgtttca
     3301 tggtgcaacg attccatttt taatgttctt tctttaccaa ctgatgttta tcgtgattac
     3361 cgttcctttg atgacaggag cttttgcagg gagattgaat ttaaaaggct atattatact
     3421 tgttattttt tggaatctgc tgatttattt ccctgtttgc cactggatat ggggtggcgg
     3481 atttttggat cagatgggat ttagagattt cgctggtgga gcagtcatcc acacgacagc
     3541 tggattcggc tctttagcat gtatcctcac acttggtcaa agaaagattc ctttaggaga
     3601 aagaagtcag catagcagtc ttatggcagc tgctatcggt acaggactgc tttggtttgg
     3661 ctggttcggt tttaattccg gtggtgcact tcgagcagat gaacaagcag tcaatgcatt
     3721 tggttcaaca ttcatcgctc ttgcatttgc gatgattact tggctgatca ttgcaaaagt
     3781 aaaaggaaat ggctttgatt ttgtcgacgt actaacagga tccgttgctg gattggcagc
     3841 cattacgcct tgtgccggtt atgtagaagc aaaaagtgcc atgctgattg ggatcattgc
     3901 gggtatcgtc tgtcatgctg ctgttgattt tcgtaaaaag aaacaatggg atgatgcact
     3961 tgatgtgtgg ggtgttcatg gcatgggcgg attcacaggt acgattctga tagggatctt
     4021 tgcatctaac agccatttac taacgaatcc aagcagctgg tacttcttag ggattcaagt
     4081 gattggtgtc atcattactg ccgtgtatgc ctatgtgctg acaacagtga ttttaaaatc
     4141 ggcgatgcac tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt
     4201 tttcttcttt ggcaacaaac tcaattgaaa caaattcaaa tagaaaagag gaagattcat
     4261 gcaacaaagc aaaacgaaaa ttcctgcaaa aaaattaacg ttctttggct ttttggctat
     4321 gaccatctca atggtcgttt cattatatga atatcccaca ttcgcgacct caggcttcag
     4381 cttagtattt ttcttgttat taggcggttt gctatggttt atccctgtgg cattgtgcgc
     4441 agcagagatg gcaacggtga aaggatggga aaaaggtgtt tcccaaacaa aaagatatta
     4501 agaaacagcc catgtttacc tgcttgcaaa caaaagaata ttgctactag actaagagat
     4561 actacataag tgtgttggta tctctttttt gttgccgtga aacagcagac tatttcattt
     4621 ctgcttgatt accttaaaac ttattagtct atggtttgtc aagggcttgt tgcgtgagaa
     4681 atgctgaata tcacccttta caaaacatag gctgataagt aacttttcaa aagcagaaaa
     4741 gaaatggcaa tgcttataac aatatgatat aatgcagatg aagacaaaaa aggaatttgt
     4801 cgagctggtt agttcgagat aattttgagt ttgttaagat gtggtgcact tgatggaatc
     4861 acaggtacga catcatatta tggaggatga aatagatgat aggtttgatt gttgcgaggt
     4921 caaagaataa tgttataggc aagaatggta atataccatg gaaaataaag ggagaacaaa
     4981 agcaatttag agagttaaca acgggtaatg tggttattat ggggcgaaag tcttatgaag
     5041 aaatcggtca tccgttgcct aatagaatga atattgttgt ttccaccaca acagagtatc
     5101 aaggagataa tttagtttca gttaaatcat tagaagatgc attattattg gctaaaggac
     5161 gagatgtata catatctggt ggatatggac tatttaagga agctttgcaa atagtagata
     5221 aaatgtatat cacagaagta gatttaaata ttgaagatgg agatacattc tttccagaat
     5281 ttgatatcaa tgattttgaa gttttgatag gggaaacact tggtgaggaa gtgaaatata
     5341 cgagaacatt ttatgtaagg aaaaaatgaa ttgagtaaat tttggattta gggtgttttc
     5401 ataaatatat tgctttttat gcatatataa attgttgtaa gacaaattcc agtttgtatg
     5461 gggcgataaa atcagaattt gagagaggat tgaatatgaa taaggaatgg tctgaactta
     5521 ataaaacaat gcaggcacaa ataaaaaaga aagatactta taagaggggt attgatactt
     5581 tacttacttt acgaagtcag ttaatacaaa ccttagtatc tttcaaagaa gaattatgca
     5641 gagaagattt taactcaatc cccttcataa acgctgatgg ttatcatagt aagacgattg
     5701 cttattctat ttggcatatt ttccgcattg aagatatagt tgtgcataca gtgataaatg
     5761 aagatgaaca agtatttttt gcaggcaatt atcaagagcg tatcaattca cctatcatta
     5821 caacaggaaa tgaactcatg aaacagcaga ttgcagactt ttcaaaacag cttaatctgg
     5881 aagaattata tttatatatt ttcgaagtat gggaaagcac tgagaaaatg cttgaacggt
     5941 tatcttatga tgaattgaaa agaaaaatac cgaaagaaag aaaaggatac ttagaatcgt
     6001 tgaatgtagt aaacgacaat gaaaaagcaa tttggctgat tgattattgg tgtaataaag
     6061 atatttgtgg actaattcaa atgccgtttt caagacattg gattatgcac acagaagcct
     6121 gtttgcgtat aaagaacaag atacattcat aggcaaaaat ttcagtttgt agaactaagg
     6181 aaattggaat ttaaggagga ataaagtatg gttagtgatt ttataagagc agcatttcca
     6241 tggattttaa tgggtttatt tgtagcaatc agttgttccc ttatgagtaa aaaagagaaa
     6301 taaattcctg tttgtattta taaaagagaa aaaatgagaa aagtagtttt atttattgcc
     6361 atgagccttg acggatatat tgcggacggt aatggtggag tagcttggct aaatggtcat
     6421 ggaaatgaca atgaaaacat cgacacttat acagaattta ccaaagatat agatactgtc
     6481 ttaatggggt ggaatacttc tcatcaggtt gttactgaac tttctccgca agaatgggta
     6541 tataataaat ttacaactta tgtattaaca cataaagagt gcaattcttc ccaacaaatt
     6601 cattttacaa gtgaaaatcc tgttttatta ttggaacggc taaaacaaga agcaggaaag
     6661 gatatttgga tttgtggcgg agcaaatctt gttcagcagt tggtaagcaa aaatatgatt
     6721 gataaatatt acatttctgt tattcctact ctgttaggaa atggggttcg tcttttgggc
     6781 aacatagaca gggaaataaa gttaaggctt tgtaaaacac agacttataa cggaataacc
     6841 gatttgattt atatgcgtag ataactagag agaaacagaa aagctgttaa accgacggag
     6901 cgatccgacc acgttggcgg gaaaatcctt aagatttttc cgccatgctt gcaagggggc
     6961 ggttcgcttc ggcggttcgg cgagcttgcg agccgttcaa agcccccgtt atctaacaag
     7021 gggggcacaa aaaacaagaa acactataca ctaacaagcg gaaaacgctg tatttataag
     7081 gcaaaacccc atttttacag tagtgatata aattgtacct atatcactat gaaaattgaa
     7141 tagtaaatat gagaaagaca gttgatttca aaaaataaga aattgaggct ctttgtcaaa
     7201 taggactgat agagtgagat aagaa
//
LOCUS       DAJSVF010000288         7225 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.117, whole genome shotgun sequence.
ACCESSION   DAJSVF010000288 DAJSVF010000000
VERSION     DAJSVF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.117"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(472..645)
                     /locus_tag="ON550_003181"
     CDS             complement(472..645)
                     /locus_tag="ON550_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3956165.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            1062..1628
                     /gene="amaP"
                     /locus_tag="ON550_003182"
     CDS             1062..1628
                     /gene="amaP"
                     /locus_tag="ON550_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3956166.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            1641..1829
                     /locus_tag="ON550_003183"
     CDS             1641..1829
                     /locus_tag="ON550_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3956167.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1842..2399
                     /locus_tag="ON550_003184"
     CDS             1842..2399
                     /locus_tag="ON550_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3956168.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            2431..2673
                     /locus_tag="ON550_003185"
     CDS             2431..2673
                     /locus_tag="ON550_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3956169.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            3014..4228
                     /locus_tag="ON550_003186"
     CDS             3014..4228
                     /locus_tag="ON550_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3956170.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            4259..4477
                     /locus_tag="ON550_003187"
                     /pseudo
     CDS             4259..4477
                     /locus_tag="ON550_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4896..5369
                     /gene="dfrF"
                     /locus_tag="ON550_003188"
                     /pseudo
     CDS             4896..5369
                     /gene="dfrF"
                     /locus_tag="ON550_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
     gene            5496..6152
                     /locus_tag="ON550_003189"
     CDS             5496..6152
                     /locus_tag="ON550_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3956171.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            6334..6864
                     /locus_tag="ON550_003190"
     CDS             6334..6864
                     /locus_tag="ON550_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3956172.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         2517 a         1056 c         1479 g         2173 t
ORIGIN      
        1 ccagttattt tagattctat gtgtgtctct tctataatgc tagtttagat gcctgggatt
       61 atattggaca atacagttgg aggatttttt attatctagc gaagagaagt gtaagttaac
      121 agaactgtat gagaaaaaag acaactacgt agtagctaac cgattcatta attttttcaa
      181 gttcatgttt atttacgtat cagaattttt cttattcaat ggtttggtgt aatcaccact
      241 tatttgtcct ttattgctta tacaatgtta tacaaaagga aaatcaagca ttgacgcatg
      301 gggtggtact atctagctga aatagcttaa taagcgcaag tagatgcatt ggattagttt
      361 ataaatcaaa agtgttagag gtattaagaa gaagtagaaa aataaaatgg attgccgaat
      421 aaaaagtacc ataaatccta aaacatgtgc caacagatag atggcacatg tttatttttt
      481 tcgataattt tgtaacaaaa atacgaagat gaaaaagaat agagattccc ataatgtagt
      541 agtccaactg aattctttac tgataccaaa taccatattt cctagatttg atacaatcaa
      601 atagccaatc gttatgatca atgaatagta caatcttttc atcataggac atacctcgtt
      661 tttatttata agaatagtat aaaccaaaga agtgaataag aaaaatgata atataaaaat
      721 aaagtaagaa cgcattaaag taactaaagt tatttattgt attttttaat tctccgaaaa
      781 aatttaataa ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa
      841 tcttgaataa tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc
      901 aattgttatt tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt
      961 agcgctttat ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag
     1021 agttatttac agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa
     1081 taatcggtgt tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca
     1141 atgcgccgta tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt
     1201 actacatgca acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca
     1261 tcttgctagt cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag
     1321 atggcaaact aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg
     1381 aagaagagtt cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata
     1441 tccatgtcaa cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga
     1501 tgcaagatat cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg
     1561 cagtcacata cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag
     1621 tggaatagga gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct
     1681 ttgggggtct tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat
     1741 tgttgatcat aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag
     1801 gaatagttga tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga
     1861 atacaacaaa tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg
     1921 aattgactta tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag
     1981 atggtctatt gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca
     2041 cagataacgt aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc
     2101 tagatgtgat cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat
     2161 tgatcagtac tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg
     2221 ttgcagacat caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag
     2281 taacagaagc agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat
     2341 ctgctgtagg caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag
     2401 aagtaagaag tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc
     2461 ggtatcattg gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc
     2521 gcaaacatca ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg
     2581 ggaccaacaa ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta
     2641 ttagtcgttt cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa
     2701 atgaagaaca aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta
     2761 tatgaagtat tctttattcc ttatgaaaaa atatctttaa aaataaaaaa agacgaaaat
     2821 aaacggtttt ctataaaaag aagaccgttt attttttgtc tttatttgtg tgaaaaacga
     2881 aaaagagcac aacacatcaa gcttttatta attgtctgtc ttttataaat gcaagtatta
     2941 attcaaaaaa acagaagaaa aaaacaacta caccttcttt tatactcgta atcgtagaaa
     3001 ggaggcgaaa aaaatgaata cattacctat caatacagga gatacagcat tcatgatttt
     3061 atgtacagca atggtttgcc tgatgactcc tggtctagct tttttttacg gcggattagc
     3121 acggaagaat aatatactaa cgatcatgtc tcaatctttt ctctccgttg ggataacaac
     3181 gattatgtgg gtatttggtg gattcggatt ggcctttgga cgggatatcg gcggtgttat
     3241 tggaaacccg gcagatttct ttttgatgag acatgttaca agcttcccca acatgtttca
     3301 tggtgcaacg attccatttt taatgttctt tctttaccaa ctgatgttta tcgtgattac
     3361 cgttcctttg atgacaggag cttttgcagg gagattgaat ttaaaaggct atattatact
     3421 tgttattttt tggaatctgc tgatttattt ccctgtttgc cactggatat ggggtggcgg
     3481 atttttggat cagatgggat ttagagattt cgctggtgga gcagtcatcc acacgacagc
     3541 tggattcggc tctttagcat gtatcctcac acttggtcaa agaaagattc ctttaggaga
     3601 aagaagtcag catagcagtc ttatggcagc tgctatcggt acaggactgc tttggtttgg
     3661 ctggttcggt tttaattccg gtggtgcact tcgagcagat gaacaagcag tcaatgcatt
     3721 tggttcaaca ttcatcgctc ttgcatttgc gatgattact tggctgatca ttgcaaaagt
     3781 aaaaggaaat ggctttgatt ttgtcgacgt actaacagga tccgttgctg gattggcagc
     3841 cattacgcct tgtgccggtt atgtagaagc aaaaagtgcc atgctgattg ggatcattgc
     3901 gggtatcgtc tgtcatgctg ctgttgattt tcgtaaaaag aaacaatggg atgatgcact
     3961 tgatgtgtgg ggtgttcatg gcatgggcgg attcacaggt acgattctga tagggatctt
     4021 tgcatctaac agccatttac taacgaatcc aagcagctgg tacttcttag ggattcaagt
     4081 gattggtgtc atcattactg ccgtgtatgc ctatgtgctg acaacagtga ttttaaaatc
     4141 ggcgatgcac tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt
     4201 tttcttcttt ggcaacaaac tcaattgaaa caaattcaaa tagaaaagag gaagattcat
     4261 gcaacaaagc aaaacgaaaa ttcctgcaaa aaaattaacg ttctttggct ttttggctat
     4321 gaccatctca atggtcgttt cattatatga atatcccaca ttcgcgacct caggcttcag
     4381 cttagtattt ttcttgttat taggcggttt gctatggttt atccctgtgg cattgtgcgc
     4441 agcagagatg gcaacggtga aaggatggga aaaaggtgtt tcccaaacaa aaagatatta
     4501 agaaacagcc catgtttacc tgcttgcaaa caaaagaata ttgctactag actaagagat
     4561 actacataag tgtgttggta tctctttttt gttgccgtga aacagcagac tatttcattt
     4621 ctgcttgatt accttaaaac ttattagtct atggtttgtc aagggcttgt tgcgtgagaa
     4681 atgctgaata tcacccttta caaaacatag gctgataagt aacttttcaa aagcagaaaa
     4741 gaaatggcaa tgcttataac aatatgatat aatgcagatg aagacaaaaa aggaatttgt
     4801 cgagctggtt agttcgagat aattttgagt ttgttaagat gtggtgcact tgatggaatc
     4861 acaggtacga catcatatta tggaggatga aatagatgat aggtttgatt gttgcgaggt
     4921 caaagaataa tgttataggc aagaatggta atataccatg gaaaataaag ggagaacaaa
     4981 agcaatttag agagttaaca acgggtaatg tggttattat ggggcgaaag tcttatgaag
     5041 aaatcggtca tccgttgcct aatagaatga atattgttgt ttccaccaca acagagtatc
     5101 aaggagataa tttagtttca gttaaatcat tagaagatgc attattattg gctaaaggac
     5161 gagatgtata catatctggt ggatatggac tatttaagga agctttgtaa atagtagata
     5221 aaatgtatat cacagaagta gatttaaata ttgaagatgg agatacattc tttccagaat
     5281 ttgatatcaa tgattttgaa gttttgatag gggaaacact tggtgaggaa gtgaaatata
     5341 cgagaacatt ttatgtaagg aaaaaatgaa ttgagtaaat tttggattta gggtgttttc
     5401 ataaatatat tgctttttat gcatatataa attgttgtaa gacaaattcc agtttgtatg
     5461 gggcgataaa atcagaattt gagagaggat tgaatatgaa taaggaatgg tctgaactta
     5521 ataaaacaat gcaggcacaa ataaaaaaga aagatactta taagaggggt attgatactt
     5581 tacttacttt acgaagtcag ttaatacaaa ccttagtatc tttcaaagaa gaattatgca
     5641 gagaagattt taactcaatc cccttcataa acgctgatgg ttatcatagt aagacgattg
     5701 cttattctat ttggcatatt ttccgcattg aagatatagt tgtgcataca gtgataaatg
     5761 aagatgaaca agtatttttt gcaggcaatt atcaagagcg tatcaattca cctatcatta
     5821 caacaggaaa tgaactcatg aaacagcaga ttgcagactt ttcaaaacag cttaatctgg
     5881 aagaattata tttatatatt ttcgaagtat gggaaagcac tgagaaaatg cttgaacggt
     5941 tatcttatga tgaattgaaa agaaaaatac cgaaagaaag aaaaggatac ttagaatcgt
     6001 tgaatgtagt aaacgacaat gaaaaagcaa tttggctgat tgattattgg tgtaataaag
     6061 atatttgtgg actaattcaa atgccgtttt caagacattg gattatgcac acagaagcct
     6121 gtttgcgtat aaagaacaag atacattcat aggcaaaaat ttcagtttgt agaactaagg
     6181 aaattggaat ttaaggagga ataaagtatg gttagtgatt ttataagagc agcatttcca
     6241 tggattttaa tgggtttatt tgtagcaatc agttgttccc ttatgagtaa aaaagagaaa
     6301 taaattcctg tttgtattta taaaagagaa aaaatgagaa aagtagtttt atttattgcc
     6361 atgagccttg acggatatat tgcggacggt aatggtggag tagcttggct aaatggtcat
     6421 ggaaatgaca atgaaaacat cgacacttat acagaattta ccaaagatat agatactgtc
     6481 ttaatggggt ggaatacttc tcatcaggtt gttactgaac tttctccgca agaatgggta
     6541 tataataaat ttacaactta tgtattaaca cataaagagt gcaattcttc ccaacaaatt
     6601 cattttacaa gtgaaaatcc tgttttatta ttggaacggc taaaacaaga agcaggaaag
     6661 gatatttgga tttgtggcgg agcaaatctt gttcagcagt tggtaagcaa aaatatgatt
     6721 gataaatatt acatttctgt tattcctact ctgttaggaa atggggttcg tcttttgggc
     6781 aacatagaca gggaaataaa gttaaggctt tgtaaaacac agacttataa cggaataacc
     6841 gatttgattt atatgcgtag ataactagag agaaacagaa aagctgttaa accgacggag
     6901 cgatccgacc acgttggcgg gaaaatcctt aagatttttc cgccatgctt gcaagggggc
     6961 ggttcgcttc ggcggttcgg cgagcttgcg agccgttcaa agcccccgtt atctaacaag
     7021 gggggcacaa aaaacaagaa acactataca ctaacaagcg gaaaacgctg tatttataag
     7081 gcaaaacccc atttttacag tagtgatata aattgtacct atatcactat gaaaattgaa
     7141 tagtaaatat gagaaagaca gttgatttca aaaaataaga aattgaggct ctttgtcaaa
     7201 taggactgat agagtgagat aagaa
//
LOCUS       DAJSVF010000289         6077 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.128, whole genome shotgun sequence.
ACCESSION   DAJSVF010000289 DAJSVF010000000
VERSION     DAJSVF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.128"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..2318
                     /locus_tag="ON550_003191"
     CDS             <1..2318
                     /locus_tag="ON550_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3956173.1"
                     /translation="LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFS
                     IGKNRRWFGGILFYFGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVG
                     GGMIGAILYHFTYFLIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQEL
                     LEGDPAKQARKQAAKEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTA
                     DEWQEPENTEPEQLSFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGT
                     SLEFEIEAEQENQDYELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKV
                     VKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVP
                     NGKISMVSFREIIEAQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKS
                     VAINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKV
                     VQEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVAS
                     NEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTI
                     IDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMM
                     PTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAH
                     GVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            2548..3615
                     /gene="rlmN"
                     /locus_tag="ON550_003192"
     CDS             2548..3615
                     /gene="rlmN"
                     /locus_tag="ON550_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3956174.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3808..4911)
                     /locus_tag="ON550_003193"
     CDS             complement(3808..4911)
                     /locus_tag="ON550_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3956175.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5464..6012
                     /gene="aac(6')"
                     /locus_tag="ON550_003194"
     CDS             5464..6012
                     /gene="aac(6')"
                     /locus_tag="ON550_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3956176.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2014 a         1051 c         1309 g         1703 t
ORIGIN      
        1 ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa
       61 atacttacca aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga
      121 atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg
      181 gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata
      241 tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata
      301 acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac
      361 agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta
      421 atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat
      481 tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga
      541 aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag
      601 aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg
      661 aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt
      721 ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga
      781 ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat
      841 ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg
      901 attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg
      961 tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag
     1021 gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg
     1081 tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac
     1141 cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt
     1201 cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt
     1261 taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac
     1321 tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta
     1381 tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg
     1441 aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa
     1501 aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg
     1561 cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg
     1621 aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg
     1681 atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg
     1741 ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca
     1801 caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag
     1861 attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc
     1921 tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc
     1981 acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga
     2041 aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc
     2101 tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc
     2161 aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc
     2221 atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag
     2281 aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga
     2341 aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt
     2401 tccatctttt ctaatacggt atgatgtctt gtctttttag tttttgtgag attaaagttc
     2461 tattcgattt atttgtgaag tattgcatta aggaaatgaa tcatgtatag tatgtagcga
     2521 aactttgaga aaaagaggga aaatactttg gaaaaacagt ccatttatgg cttgacgaat
     2581 gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt
     2641 tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa
     2701 caattgatcg aaaaattatc ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc
     2761 caagaagctt cggatggtac agtaaaatat ttgtttgaac tccctgataa aaacatgatt
     2821 gagactgtac tgatgagaca agaatatggc ttgtcagttt gtgtgacgac acaagtagga
     2881 tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg tgatctgact
     2941 gctggagaaa ttgttgcaca gatcatgatg gtccaacatt attttgatga gcgcaatctt
     3001 ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg agccatttga taattacgac
     3061 aatgtaatgg actttttgca tatcatcaat gatgccaaag gattggcaat tggtgctcgg
     3121 cacattacag tttccactag tggattagca cacaaaatca aggaattcgc taataacggc
     3181 ttgcaagtca atctggctat ttccttgcac gcgccgaata atgaggtgcg aacatcgatc
     3241 atgcggatca accgaagttt cccaattgag aaattgatgg aagctgtgga tgagtattta
     3301 gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt aaatgataga
     3361 ccagagcatg cgcaacaatt agcagatcta ttaaaagata agaaaaaatt agcatatgtc
     3421 aacttgattc catataatcc agtcagtgaa catgatcaat attctcgcag tccaaaagct
     3481 gatgtattga gattttatga tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa
     3541 gaacatggta cagatattga tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa
     3601 acagttgcca aataatcgta tcctactcgt tttttcaatt accataaaag gagcagtttt
     3661 cttaattaag gaactgttca acttaaataa gagaaaagat tctatagcga aagctataaa
     3721 gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc
     3781 ctgcttgatg ggggttttag caattattta actgattttc ttatatcctt gctggctttc
     3841 ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat
     3901 ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa
     3961 atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat
     4021 aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac
     4081 accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc
     4141 gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa
     4201 tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc
     4261 ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc
     4321 gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga
     4381 ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc
     4441 ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc
     4501 gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga
     4561 accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt
     4621 atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg
     4681 ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac
     4741 aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt
     4801 cttccctcca atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc
     4861 atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc
     4921 cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt
     4981 agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct
     5041 tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata
     5101 atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta
     5161 aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg
     5221 gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca
     5281 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
     5341 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
     5401 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
     5461 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
     5521 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
     5581 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
     5641 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
     5701 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
     5761 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
     5821 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
     5881 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
     5941 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
     6001 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
     6061 atttacgaaa attactt
//
LOCUS       DAJSVF010000290         5982 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.132, whole genome shotgun sequence.
ACCESSION   DAJSVF010000290 DAJSVF010000000
VERSION     DAJSVF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.132"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            32..208
                     /locus_tag="ON550_003195"
                     /pseudo
     CDS             32..208
                     /locus_tag="ON550_003195"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(293..3217)
                     /locus_tag="ON550_003196"
     CDS             complement(293..3217)
                     /locus_tag="ON550_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR3956177.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3363..3938
                     /locus_tag="ON550_003197"
     CDS             3363..3938
                     /locus_tag="ON550_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3956178.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4152..4847
                     /gene="vanR"
                     /locus_tag="ON550_003198"
     CDS             4152..4847
                     /gene="vanR"
                     /locus_tag="ON550_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3956179.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4825..5976
                     /locus_tag="ON550_003199"
     CDS             4825..5976
                     /locus_tag="ON550_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3956180.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCCKVLNKE"
BASE COUNT         1851 a         1132 c         1132 g         1867 t
ORIGIN      
        1 gattttattc tttatttaaa actttgcaac agaacccttt tggggtaccc tcaaggtgga
       61 gatgtttaac ctggatacgt ttgacagacc agcgtactta gaccggaaaa tcaaggcata
      121 catcgcgttc ttcaacaatg aacgcgttac tttggatatg ggattagcaa ttcctacgga
      181 agagaagatt ctacaaatga ttgcataaac gctacaaaaa aagaccacac atttttacat
      241 gcatggtcta aaatactttg tttttttacc tgtctacttg acagggggca gttcactctg
      301 agttaaaatg gtattctcct agtaaattaa tatgttccca acctaagggc gacatatggt
      361 gtaacaaatc ttcattaaag ctacctgtcc gttttttata ttcaactgct gttgttaggt
      421 ggagagtatt ccaaatactt atagcattga taattatgtt taaagcactg gctctttgca
      481 attgatgctg tatggtgcgt tctctaagct caccttgttt tccgaagaaa atagctcttg
      541 ccaatccatt catggcttct cctttattca atcctctttg tatttttctt cttaatgatt
      601 catccgatat ataattcaaa ataaagatcg ttttttctat tcggcccatc tcacgtaagg
      661 ctgtagctaa gctgttttgt cttgaatagg aacctagctt ccccataata agggatgctg
      721 aaactgttcc ctcccttata gaatgagcta atcgcaaaac atcctcataa ttttctttaa
      781 tgacctttgt atttatttgt ccacgtaaaa tggcttctag ttttggatac tcacttgctt
      841 tatctatcgt aaataatttt gagtccgata aatcccttat tcttggggca aatttaaatc
      901 ctaataaatg agtcagtccg aatatttggt cagtgtaacc ggcagtgtct gtataatgtt
      961 cctctatgtt tagatccgtc tcatgatgta acaaaccatc caaaacatga atcgcatctc
     1021 ttgaattagt atgaataatc tttgtgtagt aagaagagaa ttgatcactt gtaaatcggt
     1081 agatggtggc tccttttcca gttccataat gtggatttgc atctgcatgt agtgatgaaa
     1141 cacctagctg cattctcata ccatctgacg aagatgttgt accgtcgccc caatagaaag
     1201 gcaattgtaa tttatgatga aagtttacta atatggcttg ggctttattc atggcatctt
     1261 catacatgcg ccattgagat acattggcta gttgcttata tgtaagtccg ggtgtggctt
     1321 cggccatctt gctcaagcca atattcattc ccattcctaa aagggcagcc atgataatga
     1381 ttgtttcttc cttatctggt tttcgattat tggaagcatg agtgaattgc tcatgaaatc
     1441 ctgttatatg ggccacatcc atgagtaaat cagttaattt tattcttggt agcatctgat
     1501 aaaggcttgc actaaatttt tttgcttctt ctggaacatc tttttctaag cgtgcaagtg
     1561 atagctttcc tttttcaaga gaaaccccat ctaacttatt ggaattggca gctaaccact
     1621 ttaacctttc attaaagctg ctggttctct ccgttatata atcttcgaat gataaactaa
     1681 ctgataatct cgtattcccc ttcgattgat tccatgtatc ttccgaaaac aaatattcct
     1741 caaaatccct atattgtctg ctgccaacaa tggaaacatc tcctgcccga acatgctccc
     1801 gaagttctgt taaaacagcc atttcatagt aatgacgatt aattgttgta ccatcatcct
     1861 cgtataaatg tcttttccat cgttttgaaa taaaatccac aggtgagtca tcaggcactt
     1921 ttcgctttcc agattcgttc attcctcgga taatctcaac agcttgtaaa agtggctcat
     1981 ttgcctttgt agaatgaaat tccaatactc ttaatagcgt tggcgtatat tttcttagtg
     2041 aataaaaccg tttttgcagt aagtctaaat aatcatagtc ggcaggacgt gcaagttcct
     2101 gagcctcttc tactgaagag acaaaggtat tccattcaat aaccgattct aaaaccttaa
     2161 aaacgtctaa tttttcctct cttgctttaa ttaatgcttg tccgatgttc gtaaagtgta
     2221 taactttctc atttagcttt ttaccgtttt gtttctggat ttcctcttga gccttacgac
     2281 cttttgataa caaactaagt atttgcctat catgaatttc aaacgcttta tccgttagct
     2341 cctgagtaag ttgtaataaa tagatggtta atatcgaata acgtttattt tcttgaaagt
     2401 cacggaatgc atacggctcg tatcttgagc ctaagcgaga cagctgcaac aggcggttac
     2461 ggtgcaaatg actaatttgc actgtttcta aatccattcc tcgtatgtat tcgagtcgtt
     2521 ctattatttt tagaaaagtt tcgggtgaag gatgacccgg tggctctttt aaccaaccca
     2581 atatcgtttt attggattcg gatggatgct gcgaggtaat aatcccttca agcttttctt
     2641 tttgctcatt tgttagagat ttactaaccg tattaaatag cttcttttca gccattgccc
     2701 ttgcttccca caccattctt tcaagtgtag tgatagcagg cagtataatt ttgttttttc
     2761 ttagaaaatc tatgcattca tgcagtagat gaatggcatc accattttcc aaagctaatt
     2821 gatgaaggta cttaaatgtc attcgatatt cactcagggt aaaagttaca aagtcgtatt
     2881 cacttcgaat ttctttcaaa tgatcccaaa gtgtattttc cctttgagga taatgatcaa
     2941 gcgaggatgg actaacacca atctgtttcg atatatattg tatgaccgaa tctgggatgc
     3001 ttttgatatg agtgtatggc caaccgggat accgaagaac agctaattga acagcaaatc
     3061 ctaaacggtt ttcttccctc cttcgcttat taactatttc taaatcccgt ttggaaaaag
     3121 tgaagtaggt ccccagtatc cattcatctt cagggatttg cataaaagcc tgtctctgtt
     3181 ccggtgtaag caattctcta cctctcgcaa ttttcattca gtatcattcc atttctgtat
     3241 tttcaattta ttagttcaat tatatatcaa tagagtgtac tctattgata caaatgtagt
     3301 agactgataa aatcatagtt aagagcgtct cataagactt gtctcaaaaa tgaggtgata
     3361 ttttgcggaa aatcggttat attcgtgtca gttcgactaa ccagaatcct tcaagacaat
     3421 ttcagcagtt gaacgagatc ggaatggata ttatatatga agagaaagtt tcaggagcaa
     3481 caaaggatcg cgagcaactt caaaaagtgt tagacgattt acaggaagat gacatcattt
     3541 atgttacaga cttaactcga atcactcgta gtacacaaga tctatttgaa ttaatcgata
     3601 acatacgaga taaaaaggca agtttaaaat cactaaaaga tacatggctt gatttatcag
     3661 aagataatcc atacagccaa ttcttaatta ctgtaatggc tggtgttaac caattagagc
     3721 gagatcttat tcggatgaga caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt
     3781 ttaaaggtcg attaaagaag tatcataaaa atcacgcagg aatgaattat gcggtaaagc
     3841 tatataaaga aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg
     3901 cttcattata caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg
     3961 gcaatatttt taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg
     4021 aaaagccctt tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt
     4081 aaatgcttat aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa
     4141 gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt
     4201 tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag
     4261 aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc
     4321 ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta
     4381 tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg
     4441 atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt
     4501 tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact
     4561 ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc
     4621 ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta
     4681 gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca
     4741 ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga
     4801 aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat
     4861 tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta
     4921 ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt
     4981 ttggaaaaca aatatgactt aaatcacctg gacgcgatga aattatatca atattccata
     5041 cggaacaata tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt
     5101 cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta
     5161 cttattcaga acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa
     5221 aagctcaaca cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa
     5281 caaagaaaaa atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc
     5341 attatcggtt atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca
     5401 aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt
     5461 tttgagatta cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta
     5521 tactatatgc tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa
     5581 caggcggtta ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg
     5641 agagtcttta acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt
     5701 gacattaccg cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc
     5761 atcccaaaag ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt
     5821 tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag
     5881 catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt
     5941 ccagggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DAJSVF010000291         4879 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.147, whole genome shotgun sequence.
ACCESSION   DAJSVF010000291 DAJSVF010000000
VERSION     DAJSVF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.147"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            32..208
                     /locus_tag="ON550_003200"
                     /pseudo
     CDS             32..208
                     /locus_tag="ON550_003200"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(293..3217)
                     /locus_tag="ON550_003201"
     CDS             complement(293..3217)
                     /locus_tag="ON550_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR3956181.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3363..3938
                     /locus_tag="ON550_003202"
     CDS             3363..3938
                     /locus_tag="ON550_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3956182.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4152..4841
                     /gene="vanR"
                     /locus_tag="ON550_003203"
     CDS             4152..4841
                     /gene="vanR"
                     /locus_tag="ON550_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3956183.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKI"
BASE COUNT         1503 a          919 c          886 g         1571 t
ORIGIN      
        1 gattttattc tttatttaaa actttgcaac agaacccttt tggggtaccc tcaaggtgga
       61 gatgtttaac ctggatacgt ttgacagacc agcgtactta gaccggaaaa tcaaggcata
      121 catcgcgttc ttcaacaatg aacgcgttac tttggatatg ggattagcaa ttcctacgga
      181 agagaagatt ctacaaatga ttgcataaac gctacaaaaa aagaccacac atttttacat
      241 gcatggtcta aaatactttg tttttttacc tgtctacttg acagggggca gttcactctg
      301 agttaaaatg gtattctcct agtaaattaa tatgttccca acctaagggc gacatatggt
      361 gtaacaaatc ttcattaaag ctacctgtcc gttttttata ttcaactgct gttgttaggt
      421 ggagagtatt ccaaatactt atagcattga taattatgtt taaagcactg gctctttgca
      481 attgatgctg tatggtgcgt tctctaagct caccttgttt tccgaagaaa atagctcttg
      541 ccaatccatt catggcttct cctttattca atcctctttg tatttttctt cttaatgatt
      601 catccgatat ataattcaaa ataaagatcg ttttttctat tcggcccatc tcacgtaagg
      661 ctgtagctaa gctgttttgt cttgaatagg aacctagctt ccccataata agggatgctg
      721 aaactgttcc ctcccttata gaatgagcta atcgcaaaac atcctcataa ttttctttaa
      781 tgacctttgt atttatttgt ccacgtaaaa tggcttctag ttttggatac tcacttgctt
      841 tatctatcgt aaataatttt gagtccgata aatcccttat tcttggggca aatttaaatc
      901 ctaataaatg agtcagtccg aatatttggt cagtgtaacc ggcagtgtct gtataatgtt
      961 cctctatgtt tagatccgtc tcatgatgta acaaaccatc caaaacatga atcgcatctc
     1021 ttgaattagt atgaataatc tttgtgtagt aagaagagaa ttgatcactt gtaaatcggt
     1081 agatggtggc tccttttcca gttccataat gtggatttgc atctgcatgt agtgatgaaa
     1141 cacctagctg cattctcata ccatctgacg aagatgttgt accgtcgccc caatagaaag
     1201 gcaattgtaa tttatgatga aagtttacta atatggcttg ggctttattc atggcatctt
     1261 catacatgcg ccattgagat acattggcta gttgcttata tgtaagtccg ggtgtggctt
     1321 cggccatctt gctcaagcca atattcattc ccattcctaa aagggcagcc atgataatga
     1381 ttgtttcttc cttatctggt tttcgattat tggaagcatg agtgaattgc tcatgaaatc
     1441 ctgttatatg ggccacatcc atgagtaaat cagttaattt tattcttggt agcatctgat
     1501 aaaggcttgc actaaatttt tttgcttctt ctggaacatc tttttctaag cgtgcaagtg
     1561 atagctttcc tttttcaaga gaaaccccat ctaacttatt ggaattggca gctaaccact
     1621 ttaacctttc attaaagctg ctggttctct ccgttatata atcttcgaat gataaactaa
     1681 ctgataatct cgtattcccc ttcgattgat tccatgtatc ttccgaaaac aaatattcct
     1741 caaaatccct atattgtctg ctgccaacaa tggaaacatc tcctgcccga acatgctccc
     1801 gaagttctgt taaaacagcc atttcatagt aatgacgatt aattgttgta ccatcatcct
     1861 cgtataaatg tcttttccat cgttttgaaa taaaatccac aggtgagtca tcaggcactt
     1921 ttcgctttcc agattcgttc attcctcgga taatctcaac agcttgtaaa agtggctcat
     1981 ttgcctttgt agaatgaaat tccaatactc ttaatagcgt tggcgtatat tttcttagtg
     2041 aataaaaccg tttttgcagt aagtctaaat aatcatagtc ggcaggacgt gcaagttcct
     2101 gagcctcttc tactgaagag acaaaggtat tccattcaat aaccgattct aaaaccttaa
     2161 aaacgtctaa tttttcctct cttgctttaa ttaatgcttg tccgatgttc gtaaagtgta
     2221 taactttctc atttagcttt ttaccgtttt gtttctggat ttcctcttga gccttacgac
     2281 cttttgataa caaactaagt atttgcctat catgaatttc aaacgcttta tccgttagct
     2341 cctgagtaag ttgtaataaa tagatggtta atatcgaata acgtttattt tcttgaaagt
     2401 cacggaatgc atacggctcg tatcttgagc ctaagcgaga cagctgcaac aggcggttac
     2461 ggtgcaaatg actaatttgc actgtttcta aatccattcc tcgtatgtat tcgagtcgtt
     2521 ctattatttt tagaaaagtt tcgggtgaag gatgacccgg tggctctttt aaccaaccca
     2581 atatcgtttt attggattcg gatggatgct gcgaggtaat aatcccttca agcttttctt
     2641 tttgctcatt tgttagagat ttactaaccg tattaaatag cttcttttca gccattgccc
     2701 ttgcttccca caccattctt tcaagtgtag tgatagcagg cagtataatt ttgttttttc
     2761 ttagaaaatc tatgcattca tgcagtagat gaatggcatc accattttcc aaagctaatt
     2821 gatgaaggta cttaaatgtc attcgatatt cactcagggt aaaagttaca aagtcgtatt
     2881 cacttcgaat ttctttcaaa tgatcccaaa gtgtattttc cctttgagga taatgatcaa
     2941 gcgaggatgg actaacacca atctgtttcg atatatattg tatgaccgaa tctgggatgc
     3001 ttttgatatg agtgtatggc caaccgggat accgaagaac agctaattga acagcaaatc
     3061 ctaaacggtt ttcttccctc cttcgcttat taactatttc taaatcccgt ttggaaaaag
     3121 tgaagtaggt ccccagtatc cattcatctt cagggatttg cataaaagcc tgtctctgtt
     3181 ccggtgtaag caattctcta cctctcgcaa ttttcattca gtatcattcc atttctgtat
     3241 tttcaattta ttagttcaat tatatatcaa tagagtgtac tctattgata caaatgtagt
     3301 agactgataa aatcatagtt aagagcgtct cataagactt gtctcaaaaa tgaggtgata
     3361 ttttgcggaa aatcggttat attcgtgtca gttcgactaa ccagaatcct tcaagacaat
     3421 ttcagcagtt gaacgagatc ggaatggata ttatatatga agagaaagtt tcaggagcaa
     3481 caaaggatcg cgagcaactt caaaaagtgt tagacgattt acaggaagat gacatcattt
     3541 atgttacaga cttaactcga atcactcgta gtacacaaga tctatttgaa ttaatcgata
     3601 acatacgaga taaaaaggca agtttaaaat cactaaaaga tacatggctt gatttatcag
     3661 aagataatcc atacagccaa ttcttaatta ctgtaatggc tggtgttaac caattagagc
     3721 gagatcttat tcggatgaga caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt
     3781 ttaaaggtcg attaaagaag tatcataaaa atcacgcagg aatgaattat gcggtaaagc
     3841 tatataaaga aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg
     3901 cttcattata caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg
     3961 gcaatatttt taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg
     4021 aaaagccctt tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt
     4081 aaatgcttat aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa
     4141 gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt
     4201 tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag
     4261 aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc
     4321 ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta
     4381 tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg
     4441 atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt
     4501 tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact
     4561 ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc
     4621 ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta
     4681 gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca
     4741 ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga
     4801 aatatataaa aacggtatgg ggggttggtt ataaaattta aaaataaaaa aaacgactat
     4861 tccaaactag aacgaaaac
//
LOCUS       DAJSVF010000292         4604 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.153, whole genome shotgun sequence.
ACCESSION   DAJSVF010000292 DAJSVF010000000
VERSION     DAJSVF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.153"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            217..1128
                     /locus_tag="ON550_003204"
     CDS             217..1128
                     /locus_tag="ON550_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HCR3956184.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1281..1766
                     /gene="vanZ"
                     /locus_tag="ON550_003205"
     CDS             1281..1766
                     /gene="vanZ"
                     /locus_tag="ON550_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCR3956185.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2053..2382)
                     /locus_tag="ON550_003206"
     CDS             complement(2053..2382)
                     /locus_tag="ON550_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3956186.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2687..3016
                     /locus_tag="ON550_003207"
     CDS             2687..3016
                     /locus_tag="ON550_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3956187.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3006..3293
                     /locus_tag="ON550_003208"
     CDS             3006..3293
                     /locus_tag="ON550_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3956188.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3572..4135)
                     /locus_tag="ON550_003209"
     CDS             complement(3572..4135)
                     /locus_tag="ON550_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3956189.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(4328..4573)
                     /locus_tag="ON550_003210"
                     /pseudo
     CDS             complement(4328..4573)
                     /locus_tag="ON550_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
BASE COUNT         1581 a          717 c          780 g         1526 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg tatcacttat gaaaattcat
       61 ctacattggt gataatagta aatccagtag ggcgaaataa ttgactgtaa tttacggggc
      121 aaaacggcac aatctcaaac gagattgtgc cgtttaaggg gaagattcta gaaatatttc
      181 atacttccaa ctatatagtt aaggaggaga ctgaaaatga agaagttgtt ttttttattg
      241 ttattgttat tcttaatata cttaggttat gactacgtta atgaagcact gttttctcag
      301 gaaaaagtcg aatttcaaaa ttatgatcaa aatcccaaag aacatttaga aaatagtggg
      361 acttctgaaa atacccaaga gaaaacaatt acagaagaac aggtttatca aggaaatctg
      421 ctattaatca atagtaaata tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat
      481 ttatctaaac atgacgaatt aataaatgga tacgggttgc ttgatagtaa tatttatatg
      541 tcaaaagaaa tagcacaaaa attttcagag atggtcaatg atgctgtaaa gggtggcgtt
      601 agtcatttta ttattaatag tggctatcga gactttgatg agcaaagtgt gctttaccaa
      661 gaaatggggg ctgagtatgc cttaccagca ggttatagtg agcataattc aggtttatca
      721 ctagatgtag gatcaagctt gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa
      781 gaaaatgctt ggaaatacgg gttcatttta cgttatccag aggacaaaac agagttaaca
      841 ggaattcaat atgaaccatg gcatattcgc tatgttggtt taccacatag tgcgattatg
      901 aaagaaaaga atttcgttct cgaggaatat atggattacc taaaagaaga aaaaaccatt
      961 tctgttagtg taaatgggga aaaatatgag atcttttatt atcctgttac taaaaatacc
     1021 accattcatg tgccgactaa tcttcgttat gagatatcag gaaacaatat agacggtgta
     1081 attgtgacag tgtttcccgg atcaacacat actaattcaa ggaggtaagg atggcggaat
     1141 gaaaccaacg aaattaatga acagcattat tgtactagca cttttggggt aacgttagct
     1201 ttttaattta aaacccacgt taactaggac attgctatac taatgataca acttaaacaa
     1261 aagaattaga ggaaattata ttgggaaaaa tattatctag aggattgcta gctttatatt
     1321 tagtgacact aatctggtta gtgttattca aattacaata caatatttta tcagtattta
     1381 attatcatca aagaagtctt aacttgactc catttactgc tactgggaat ttcagagaga
     1441 tgatagataa tgttataatc tttattccat ttggcttgct tttgaatgtc aattttaaag
     1501 aaatcggatt tttacctaag tttgcttttg tactggtttt aagtcttact tttgaaataa
     1561 ttcaatttat cttcgctatt ggagcgacag acataacaga tgtaattaca aatactgttg
     1621 gaggctttct tggactgaaa ttatatggtt taagcaataa gcatatgaat caaaaaaaat
     1681 tagacagagt tattattttt gtaggtatac ttttgctcgt attattgctc gtttaccgta
     1741 cccatttaag aataaattac gtgtaagatg tctaaatcaa gcaatctgat ctttcataca
     1801 cataaagata ttgaatgaat tggattagat ggaaaacggg atgtggggaa actcgcccgt
     1861 aggtgtgaag tgaggggaaa accggtgata aagtaaaaag cttacctaac actatagtaa
     1921 caaagaaagc ccaattatca attttagtgc tgaggaattg gtctctttaa taaatttcct
     1981 taacgttgta aatccgcatt ttcctgacgg tacccccaaa ctaagatttc atagatagat
     2041 tttctgataa ctttaaaaaa cacgaatagc ggaaaggatg tcttcattcg ctaaagagta
     2101 ataaactact tttccatctt gacgtgattt tgccattcca tgttttttta aaaaacgtaa
     2161 atggtgtgac gttgtagcga cactagcagt aagtatttct gctaaatcac agacgcacat
     2221 ttccttatag gtttctaaag catagaaaat tttaattcta gaaaaatcgc tgaaacattt
     2281 ccctaacaca agtaagtttg aaaaatcttt ttgatccaat ttatttttta ttccatttac
     2341 tttttcttta tgtacgacag tcacttggca aacttttttc attcatctca cctcacattc
     2401 aaacgattat ttgaatgaag tatacctatt tgatcactct tttgtcaatc taccttaatt
     2461 ccaatacact attataagca atttcatgta ttgaagtaaa cttacttgca agtgcgtttt
     2521 tgattccaag ttaaaaacat attattattc tacataattt cctaatcaaa taaaactcct
     2581 ttgaaaattg ttctaaagga gttttattat tttgtatttc tactaaaaat agcgtacaat
     2641 gtaattacta agtaattacg aaatgtaaaa taatggagga aataatatga ttacaaaaac
     2701 aagaaaacaa ggaaattcta ttatgttaac ggtccctaaa gaattcgatg tgcccaatgg
     2761 tgttgaagta gaagctaagc ttgtagagaa tggtatcctt tatgagtttg tagagcctaa
     2821 aaaagaattt tttgatttta gtgaagatat tttggctgat attctttcag aaggttatag
     2881 caaacaagag atcttaaaag aatttagaaa tagaaaaaat gcgttaactt ctgcatttaa
     2941 atctattgct gaagatactg ttgcaaattc taaaccgatg acaaaagagg agttggcagc
     3001 agaaattggc ttatgagatt ctaccttcta aacacgtaat aaagtatcta aaaaaattaa
     3061 aagagaaaac attaaaagaa caatttttaa cgataattta tgatgagata gcagttcgtc
     3121 ctcattcggg agaacaaaaa acgggtgatt tatcaggaat ttgggctatt ggattcaagt
     3181 atgcaggtac gacatatcgc gttgcatatg aaattaaaga taatgcagtg atacctatct
     3241 tactatgtgg aacgcatgaa aatttttatg aacaattaaa aaagattagg taagaaccca
     3301 atgtttcaac ttgagaggat agacgacact ttaaaagata atttttaaca gctgagagtt
     3361 gtaataccct ataattttaa accactaacc tatatattta tgaaaaatag aaaaaattaa
     3421 aacgcatttt aagcctcttt caaattaaaa gcttcatcct tatgcacaaa gagggcttaa
     3481 aatgcgttat tttatattgt tttttagtaa taataaaaaa atcactctaa ttctcccata
     3541 aatattatag attcctaaag attaaaatag attattccct attgtctttt aagtagcggt
     3601 agagtgttgc cttgctaata ccagtcattt ccgtaatttc ttttacgcta taaacctgac
     3661 ttttatacaa ttttactgct cgatcaacat ctttctggtt cactcgtgga cggccacctt
     3721 tacgacctct cgcacgtgca cttttcaaac cttctttggt acgttcaaca attaaatcac
     3781 gttcgaactg actaaatgct tggaagacag tcatcattaa gcgtccgtga ggcgtgctgg
     3841 tatcgaagtt ttctttcaag ctcaccagct taacatggtg ttcttcaaaa taggtaacaa
     3901 ggtcaatcaa atcctttgta ctacgaccca atcgagaaaa actttctaca attaccgtat
     3961 ctcccggacg aattttatct tttaaacgat ttagttgggg acgaaccgct ttggttcccg
     4021 tcattttctc agttaggatt tcattacaat tttgttcatt gagtaagtct aattgacgat
     4081 ttagttcttg tgaacgtgta ctcactcggg cataaccata aatgtatcca ctcatgcttt
     4141 tcttcctttc gtactttaat tctggcttga ttgtatcata aaagggagtt atagatacat
     4201 agttaatgca acaagttatg atacaaaata tgacctcttt tatttaataa aagaggtcat
     4261 ttcattttgt ctcataatcg attgtttttg atacggtttt tagtaataaa tggagactct
     4321 tctggtatca tgaatcagaa gagtctccat ctgcatacat atgtatttta tttatagcat
     4381 ctaaactgtt ttcagcaata aaagtaggtt ttgtttcact ccacagatgt ctatatttgg
     4441 ttaaactatc aattccctct cccgttaaaa gtaaaacact gtttatacca gcatttttgg
     4501 ccaacatcat atcgttttta ccggaatcgc ctactacgaa acacttactt ggatttaaat
     4561 cgtatttttt taacagaatt aggcagaagt cgcccatcct ctat
//
LOCUS       DAJSVF010000293         2976 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.188, whole genome shotgun sequence.
ACCESSION   DAJSVF010000293 DAJSVF010000000
VERSION     DAJSVF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.188"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            297..1265
                     /locus_tag="ON550_003211"
     CDS             297..1265
                     /locus_tag="ON550_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3956190.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1258..2289
                     /gene="vanA"
                     /locus_tag="ON550_003212"
     CDS             1258..2289
                     /gene="vanA"
                     /locus_tag="ON550_003212"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3956191.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2295..2903
                     /gene="vanX"
                     /locus_tag="ON550_003213"
     CDS             2295..2903
                     /gene="vanX"
                     /locus_tag="ON550_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HCR3956192.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYAASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          885 a          570 c          719 g          802 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg cttccagcga tgccagactt
       61 ggttgataaa aggaggtcct aagagatgta tataattttt taggaaaatc tcaaggttat
      121 ctttactttt tcttaggaaa ttaacaattt aatattaaga aacggctcgt tcttacacgg
      181 tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt tgacaagatt
      241 accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc ttaattatga
      301 ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca ttccatgctc
      361 tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg gaatccaacg
      421 ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag atttccgcct
      481 ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga agcatcggct
      541 gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac aatgtggcgt
      601 actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca gtacgcaacg
      661 taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc gaccgtggca
      721 aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc aaagcggtta
      781 ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc cgaagtatag
      841 aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt acgcttcatg
      901 tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag agaatgaagc
      961 aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat gagttggtta
     1021 aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa ggagaggaag
     1081 agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta cttaaacttc
     1141 aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag caagcgttgc
     1201 gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga caggagcatg
     1261 aatagaataa aagttgcaat actgtttggg ggttgctcag aggagcatga cgtatcggta
     1321 aaatctgcaa tagagatagc cgctaacatt aataaagaaa aatacgagcc gttatacatt
     1381 ggaattacga aatctggtgt atggaaaatg tgcgaaaaac cttgcgcgga atgggaaaac
     1441 gacaattgct attcagctgt actctcgccg gataaaaaaa tgcacggatt acttgttaaa
     1501 aagaaccatg aatatgaaat caaccatgtt gatgtagcat tttcagcttt gcatggcaag
     1561 tcaggtgaag atggatccat acaaggtctg tttgaattgt ccggtatccc ttttgtaggc
     1621 tgcgatattc aaagctcagc aatttgtatg gacaaatcgt tgacatacat cgttgcgaaa
     1681 aatgctggga tagctactcc cgccttttgg gttattaata aagatgatag gccggtggca
     1741 gctacgttta cctatcctgt ttttgttaag ccggcgcgtt caggctcatc cttcggtgtg
     1801 aaaaaagtca atagcgcgga cgaattggac tacgcaattg aatcggcaag acaatatgac
     1861 agcaaaatct taattgagca ggctgtttcg ggctgtgagg tcggttgtgc ggtattggga
     1921 aacagtgccg cgttagttgt tggcgaggtg gaccaaatca ggctgcagta cggaatcttt
     1981 cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa acgcagttat aaccgttccc
     2041 gcagaccttt cagcagagga gcgaggacgg atacaggaaa cggcaaaaaa aatatataaa
     2101 gcgctcggct gtagaggtct agcccgtgtg gatatgtttt tacaagataa cggccgcatt
     2161 gtactgaacg aagtcaatac tctgcccggt ttcacgtcat acagtcgtta tccccgtatg
     2221 atggccgctg caggtattgc acttcccgaa ctgattgacc gcttgatcgt attagcgtta
     2281 aaggggtgat aagcatggaa ataggattta cttttttaga tgaaatagta cacggtgttc
     2341 gttgggacgc taaatatgcc acttgggata atttcaccgg aaaaccggtt gacggttatg
     2401 aagtaaatcg cattgtaggg acatacgagt tggctgaatc gcttttgaag gcaaaagaac
     2461 tggctgctac ccaagggtac ggattgcttc tatgggacgg ttaccgtcct aagcgtgctg
     2521 taaactgttt tatgcaatgg gctgcacagc cggaaaataa cctgacaaag gaaagttatt
     2581 atcccaatat tgaccgaact gagatgattt caaaaggata cgcggcttca aaatcaagcc
     2641 atagccgcgg cagtgccatt gatcttacgc tttatcgatt agacacgggt gagcttgtac
     2701 caatggggag ccgatttgat tttatggatg aacgctctca tcatgcggca aatggaatat
     2761 catgcaatga agcgcaaaat cgcagacgtt tgcgctccat catggaaaac agtgggtttg
     2821 aagcatatag cctcgaatgg tggcactatg tattaagaga cgaaccatac cccaatagct
     2881 attttgattt ccccgttaaa taaactttta accgttgcac ggacaaacta tataagcggt
     2941 tctgttgcaa agttttaaat aaagaataaa atccct
//
LOCUS       DAJSVF010000294         1970 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.210, whole genome shotgun sequence.
ACCESSION   DAJSVF010000294 DAJSVF010000000
VERSION     DAJSVF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.210"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            47..439
                     /locus_tag="ON550_003214"
     CDS             47..439
                     /locus_tag="ON550_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3956193.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="ON550_003215"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="ON550_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HCR3956194.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          819 a          149 c          337 g          665 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaattc aaaatctttc agagaagctg ttaaaggaat
//
LOCUS       DAJSVF010000295         1315 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052108
            SAMN29549312-rid15782233.guided.233, whole genome shotgun sequence.
ACCESSION   DAJSVF010000295 DAJSVF010000000
VERSION     DAJSVF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549312
            Sequence Read Archive: SRR20032423
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473971.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473971.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:24:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,218
            CDSs (total)                      :: 3,152
            Genes (coding)                    :: 3,078
            CDSs (with protein)               :: 3,078
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 9 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549312-rid15782233.guided.233"
                     /strain="AUSMDU00052108"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..113
                     /locus_tag="ON550_003216"
     CDS             <1..113
                     /locus_tag="ON550_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR3956195.1"
                     /translation="TAKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            162..245
                     /locus_tag="ON550_003217"
     CDS             162..245
                     /locus_tag="ON550_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HCR3956196.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            368..1105
                     /gene="erm(B)"
                     /locus_tag="ON550_003218"
     CDS             368..1105
                     /gene="erm(B)"
                     /locus_tag="ON550_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3956197.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          493 a          210 c          225 g          387 t
ORIGIN      
        1 aaacagcaaa gaatggcgga aacgtaaaag aagttatgga aataagactt agaagcaaac
       61 ttaagagtgt gttgatagtg cattatctta aaattttgta taataggaat tgaagttaaa
      121 ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc
      181 gtaatgtaga taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata
      241 aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa
      301 ctaaaattac aaacaaatcg tttaacttct gtatttattt acagatgtaa tcttcaggag
      361 tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact
      421 caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac
      481 aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga
      541 attagacagt catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac
      601 tttaattcac caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt
      661 tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagccg
      721 tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca
      781 ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc
      841 agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca
      901 taccacagat gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt
      961 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc
     1021 caaagtaaac aatttaagta ccattactta tgagcaagta ttgtctattt ttaatagtta
     1081 tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac
     1141 acgttactaa agggaatgga gataaattat tagatatact actgacagct tccaagaagc
     1201 taaagaggtc cctagcgcct acggggaatt tgtatcgata aggggtacaa attcccacta
     1261 agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg caacaatatt gtatt
//
